LOCUS       DAONVY010000001        15091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_2963_length_15091_cov_4.104283, whole genome shotgun sequence.
ACCESSION   DAONVY010000001 DAONVY010000000
VERSION     DAONVY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15091
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_15091_cov_4.104283"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLI11_00005"
     CDS             complement(<1..281)
                     /locus_tag="PLI11_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51282.1"
                     /translation="MKTLLFLVKNIFTVTFRRKSNILVYLILPIISVLVSLLIYSNTG
                     SSNMIIGYVDNDKTFFSKELLDSIETLDGYILKDVKDEDMNGLLADNKIA"
     gene            complement(278..1399)
                     /locus_tag="PLI11_00010"
     CDS             complement(278..1399)
                     /locus_tag="PLI11_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ51283.1"
                     /translation="MNTLNIIIKELKQLVRDKKSNAIMILLPIVLIFILSKAFSGNFG
                     QTLKLGDVSVLYTMNAEESLEQSFEMFMETINKELGIQFIETNKKKEGLKAVKDVKYA
                     CYIMIEENEIEIYKNERYNFSANLVESMVRSFAKRYDALNEIAKTNPQAIEAILSDEN
                     MNFTEIRSLDGKSSPSSLDYYAVTMLTLILLYSSMTGYMSIKSEQYLRTQYRVFASPV
                     RKHEYLTGKIIGCLSISVLQAMCVFIFGKYLLNANWGSDIPTVLLVILAECIMTITLG
                     ACIAVLVKKAETGIAALNIAIPIMAFLGGGYVPLSQMGGTINKLSVISPLRWTNEAIF
                     RVIYNNDYSSVPIAIGISVGIAAIAIMLTAIKTGKEKSI"
     gene            complement(1412..2350)
                     /locus_tag="PLI11_00015"
     CDS             complement(1412..2350)
                     /locus_tag="PLI11_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51284.1"
                     /translation="MSIVRIENLTKKYGNIVAVNDLSLSINEGEIFGLLGPNGAGKST
                     LINMITSLITIDEGNIYIKDTNTKSNPLLTKSFTGIVPQDIAIYEDLTCLENVKFFAG
                     LYGLRGKELKSKAMEALEFTGLSDKAKDFPKGFSGGMKRRLNIACAIAHNPEIIIMDE
                     PTVGIDPQSRNHILQSVKELNRRGCTVIYTSHYMEEVEELCSRIAIIDYGKVIALGTK
                     QELKSIVKHKNEVWVTVKNNIDKIVQEELKEIPGVTDVEIYENTVMVYSMHESENLES
                     IIAYFTSKGLGILQIESKVPDLETVFLTLTGRKLRD"
     gene            2499..3530
                     /locus_tag="PLI11_00020"
     CDS             2499..3530
                     /locus_tag="PLI11_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51285.1"
                     /translation="MKKQFIIVVLLLVLCIFVSAYTYTTDGSFEDEEVGSSYVDLDKF
                     SYDWGHSVVHGETVIQEEDGNKYLKVTGFSEFYSYDPITAPYTFSLDLKLEAVGDVNV
                     FVRAGRNEIAPFPFYEWDWYEEKGGTNGKSSTGGPGLLVSLLTDSVRIRIKNMQMDSE
                     NEQISSVYHDFPMPETYNYKEFNNIKFVDDGEKIEIFVNNELIATCDMSDKGRYEVDA
                     AHPPVDFEYFKKAVLKDSEGNVVLELDNARLVAEECRVAFGGRSHPFYVDNVAMSYEV
                     EDTPAPTDTPKPEPTDTEVPKETNESTKKPDEEKGEKADWIPFAVVCVVLVLVGVVIA
                     IIARRRAVK"
     gene            3533..5359
                     /locus_tag="PLI11_00025"
     CDS             3533..5359
                     /locus_tag="PLI11_00025"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPZ51286.1"
                     /translation="MRFSGKAFAFALSSFVMLMCTLSGCTKTYDIPDAPEGIVYLVYE
                     GKSNYKIVVGAKASKAEQYAAEELQKYIKKITGFNIPIIKDNKKETELEIVVGRTNRE
                     KDYEVDRDSLGEEGFTILWSNKKLVIAGGGDRGTIYGVYDFLESLGCRFFAEGVEKVP
                     SNKTLELQLHEPKSDKPVFEYRDLFWTNTYDPVFSTKLRLNGCLKSGENGRTIPKNLG
                     SGIFYAGPHFVHTFEHLVPASVYFDEHPEYFSEINGERTAKHLYSQLCLTNPDVLKIC
                     IDKVKEWLRENPEAKIVSVSQNDSFVIESYCTCSECKKVDREERSKSGTLIRFVNKIA
                     EAIKDEFPDVYVDTLAYQYSIEPPRVTKPLDNVIVRVCTGGCSAHPIGNCPNNSGIKG
                     CIERWAKISNRIYIWDYTTNFAQYLCPFPNLDTLQPNMQFFFENNVKGVFEQGNYQEG
                     LNGEFGELRSYILAKLLWDPYTDVEIHINEFLETYYGKASPYVKEYVEFLHEKVSAPS
                     VHFNLVVDAASLYRSLINNEELAHLDSLWEKAKEEAENERVLERVRRSELSYRFYKLT
                     SRRGEFADVFEYDRLEKEFYKDCKELGVLRLSEGANIPLVNP"
     gene            complement(5398..6249)
                     /locus_tag="PLI11_00030"
     CDS             complement(5398..6249)
                     /locus_tag="PLI11_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51287.1"
                     /translation="MIDDTNGHWNDFGLIAKNIEQVFKVANELGENSPQIAIATGGPL
                     RDGNTKIQEKELDVYWKLHNDHPGTFFQYKGKPFLIMYIAGEVNKRHSDDRFTIRHGT
                     GFISWQKKAQDQSIFQTEGNWGWVFDIQNEGSEVIGVQPGYNKAHQGIEIQTIYRENG
                     KHYIQSWLNAIKQNPEMIIIPSYNDHAEETGWEATVPIRPPSNENCDQGTEGEDPYLY
                     EKITEAYLALRYGFIDGFMYREENSKETYLFKNNKLAKVEPKETDLVIIIPDGYFEWE
                     SNKNTKK"
     gene            complement(6328..6636)
                     /locus_tag="PLI11_00035"
     CDS             complement(6328..6636)
                     /locus_tag="PLI11_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51288.1"
                     /translation="MKKLIILVVTFSLFAASCSNTSVTEKERVERVPNPTPDVIKPDN
                     KYVDVPAYDKFGQWPYKDGEEYYSSKVGTWFTVWWTDESKPTHDHWFAEGWTRLKPVD
                     "
     gene            complement(6711..7316)
                     /locus_tag="PLI11_00040"
     CDS             complement(6711..7316)
                     /locus_tag="PLI11_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPZ51289.1"
                     /translation="MSRVLYIKANAKKEGESRTFRIADSFIEAYKSFHPNDEIITLDL
                     YKEGIDFLREDDLNAVLAHKETEDREHPILKYAYQFRDADKYVIAEPLWNLSIPAILK
                     AYLDYVTVSGITFRYTEYGAEGLCKGKKAINITTRGGNYTEKGAAQYEMGDSYLRTLF
                     GFLGIVDFKTLSVDELDVIGTDVEANIKEGIKEAIRLAETF"
     gene            7793..8998
                     /locus_tag="PLI11_00045"
     CDS             7793..8998
                     /locus_tag="PLI11_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51290.1"
                     /translation="MLKKCTVLVIALCMVLALVPLRTQAADPPHSKSMIFKDNQVING
                     SVAASGNNMKSLVSGDVLKLKILGKGDPNIGIDFVENIDTSTYKILAMKVKKEYAGGG
                     LEGEIFYNVKGQGAVGGKSIIFTWAEGTDWQWIYVDLGAANAGDVGFIRFDPYGESPV
                     ENTMITVAGIGFFKTVDDMNAFAATPDGQNLGSNEGDRDFPVEVPFSGTDLNIGIWLN
                     TVSDPSKPGHYWIRFNAAAPFYGINFYLYASKNNAKGHWALYKFDTSVEQTLAGEPVA
                     REDLSLSQDTHLQAAFEPMNPGQYIFYVELEPDQDPDPTVFYYVVACSSIDAPEGLIE
                     YGFNGTKHPDYNGPLVMAGTVMFVETEGVNDYLIPLDAENPTTGDVGIIAYGFAALAA
                     VLVKRRKVK"
     gene            9411..9830
                     /locus_tag="PLI11_00050"
     CDS             9411..9830
                     /locus_tag="PLI11_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51291.1"
                     /translation="MKYAGLAKEIEELLVALSEHYSKPCGGESQEKKVYDKPDMLEVN
                     KVQYNKFKTIISFIQIISDNDNDIIQYQFGKDGLDVNVEWKTPSLYLSSYDRTIELFS
                     RVLLLADSVKVCITGLDEMSVCFKICNVMRKVEKSEE"
     gene            9827..10405
                     /locus_tag="PLI11_00055"
     CDS             9827..10405
                     /locus_tag="PLI11_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPZ51292.1"
                     /translation="MKELEYKFLVDKEFFNKAQETLKRKYKKISVNVIEQANYYYDND
                     NFELRKSGITIRIRHKDGALKLQVKKHLKTDKRYSLCYEEERSVDMVYGSITVEGKRY
                     DLKGVLHTRRTRYVIDDGIRVEFDISSYLGHEDYEIEIEFLMKRIDDVKKMIEMLDLP
                     LNKRGKGKASRFFEALKSMRDKNYDMTKPKEI"
     gene            10595..11215
                     /locus_tag="PLI11_00060"
     CDS             10595..11215
                     /locus_tag="PLI11_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51293.1"
                     /translation="MRSERMTDEELERQLRFILKQINENVNVLEDYIDEIKDDEELAE
                     LFTAVSGKTLEEMIEEQNESDRRRTEESRGYYEKLKQYMDEKNWTYRSIDDDMILVVC
                     FGLSNVVVNVFVIIEPISQTVTINTVLPYEIKDGNEDALKIMLAEFNSTCRFGSFVFD
                     DETGCLAYYYSWCFFRQNFVKEVFENYLYACLIPPDDYAEIINGII"
     gene            11275..11451
                     /locus_tag="PLI11_00065"
     CDS             11275..11451
                     /locus_tag="PLI11_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51294.1"
                     /translation="MFFGMFSGIDELLAQDTSSYQLFHSLPKEMQEKIVEHDFTSFYE
                     LNRFVQKNRHKFKG"
     gene            11626..14271
                     /locus_tag="PLI11_00070"
     CDS             11626..14271
                     /locus_tag="PLI11_00070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPZ51295.1"
                     /translation="MKITKKIVRAVFIPILLMLSAVLILIILLSMHAVNQQRQKQNSA
                     IGNARDFMERAMQHLYHNAVVLSTNGNAYGHVSGGDDNIIEVIEESGADCVCFVSSTN
                     VYERYFDTSVEDLGNDENVKSFMRSEKDTDIKFIRGKPFILAKASYEDNQFDYILLAR
                     EMDKLFLNELDRIIDGKTTVIPSAENDSEKYILELSEAAALKIDMDNGRELQVAIIAL
                     SVFVVLVVPLSVVLLWYVNRYINKPIFALTDKIRGISYSDKGETYEETYCDDEEFLEL
                     IYTIDSLITTNSNILDRLRKGDAELRLILDTVVECILGLDLEGNITYCNKRCLNMLGY
                     DCFTEILGRNIKEITGKDIQFYENNQPNICRFSGKDETLEVELYRYPKIEDNRIVGYV
                     VAIIDITEKRKMEHELTESQRSTEFLLTNIMGMVYRCRHDENWTVLYVSEGCYKLTGY
                     KAESFINNRDVTLNDIIAKPFRERIRQRYEIVLANREVFREEYIIVTADGSEKWVLEQ
                     GQGVYDDEGNVVAIEGLIIDISDQKAREKEIEYLSYHDALTGVYNRIYLDREKKRLME
                     SDMHPISVIVGDIDGLKFINDAFGHAVGDELIKRTANILKSCLREGDVIARVGGDEFN
                     ILLPKTDESEAHRIMKNMQREIKKFSSENMRLYNFTVSLGYSTKYTREDNLNDCIKQA
                     DEYMYKRKLLERHSSHSAVIASMRETMNARGIETEEHCRRLADLSREMGMAMDLSEKE
                     VNELVLLSSLHDIGKVGINDAIVKKATTMTNEEFEEMKKHPMIGYRIAMATHELQSIA
                     PYILTHHERWDGLGYPQGLKGEEIPKASRIFSVLVAYDVLTCGRPYKQPVSKEEALKE
                     IEENAGLQFDPAIAKIFVDWMRKQH"
     gene            complement(14268..>15091)
                     /locus_tag="PLI11_00075"
     CDS             complement(14268..>15091)
                     /locus_tag="PLI11_00075"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPZ51296.1"
                     /translation="FANIAHGCNSVIATKLGLKTADYVITEAGFGADLGAEKFFNIKC
                     RKANLKPSAAVIVATVRALKHHGNGDIEKGLDNLGKHIENIQCFGVPAIVAINHFAND
                     SEDDTRSIVNYCSNLGVDAEICTLHADGGKGGIKLAEKIVELAETNSKFKLLYDDNIS
                     IKEKIETICSKIYGAKGVVYTPKAQEEINTIEELGYSNLPVCMAKTQYSLSDNPKLLT
                     RPKNFEITVSDVRVSAGAGFIVVQTGNILTMPGLPKNPAAAYLDIKEDGTIFGLF"
BASE COUNT         4480 a         2621 c         3387 g         4603 t
ORIGIN      
        1 gcaattttat tatcagcgag caggccattc atatcctcat ctttaacatc tttcaagata
       61 taaccgtcca atgtttctat ggagtcaagc agctcctttg agaagaatgt cttgtcattg
      121 tcaacgtaac ctataatcat gttgctggag ccagtgttac tgtatatcaa aagggaaacc
      181 aatacactta taataggcag gatgaggtat acaagtatgt tggattttct cctgaaagtt
      241 actgtgaata tattcttaac taaaaacaga agggttttca tatgcttttt tcctttccag
      301 tttttattgc agtcagcatt attgcaatgg cagcgatgcc gacactaata ccgatggcaa
      361 tgggtacact gctgtaatca ttgttataaa ttaccctgaa tattgcctcg ttagtccatc
      421 taagaggtga aattacacta agtttgttta ttgtgccacc catctgggac aagggcacgt
      481 atccaccgcc caggaaagcc attattggaa ttgcaatgtt aagagcagca atgcctgttt
      541 cagctttttt tacaagcacc gcaatacatg cacccaaagt gatggtcatg atacattctg
      601 cgagaataac caatagcacg gtgggtatgt cactgcccca gttggcgtta agaaggtatt
      661 ttccaaatat gaagacacac atggcctgca aaacagaaat agacaaacat ccaattattt
      721 ttcctgtcag gtattcatgt ttcctaacgg gcgatgcaaa taccctgtat tgtgttctta
      781 agtactgctc gctcttgatg gacatataac ctgtcatgga cgagtacagg aggattaggg
      841 taagcatggt gactgcgtaa tagtcgaggg aacttggtga actttttccg tctaacgacc
      901 ttatctccgt aaaattcatg ttttcatcgc tcaatatggc ttcaattgcc tgggggtttg
      961 tctttgcaat ttcattcaat gcgtcatacc tcttggcaaa gcttcttacc atggattcaa
     1021 caagatttgc actaaagttg tatctctcgt ttttatatat ctctatttca ttttcctcta
     1081 tcattatata gcaggcgtat tttacatcct tgactgcttt taatccttcc tttttcttgt
     1141 ttgtttcaat aaactgaatg ccaagctcct tgtttatggt ttccataaac atttcaaagc
     1201 tctgctcaag gctttcttcc gcattcatgg tataaagcac actgacatcg ccaagtttca
     1261 gggtttgtcc aaaattgcct gagaaagcct ttgaaagaat gaaaatcagt acaattggaa
     1321 gaaggatcat gatggcattg gattttttat ccctgacaag ctgcttcaac tctttaatta
     1381 tgatatttag agtattcatt acaacctcct gtcagtccct aagttttctg cctgtcagtg
     1441 taaggaacac tgtttcaaga tccgggacct ttgattcaat ctgaagtatt ccaagccctt
     1501 ttgatgtgaa gtatgcgata atggattcca ggttctcgct ttcatgcatg ctgtatacca
     1561 tgaccgtgtt ttcatatatt tcaacatcgg ttacaccggg aatttccttc aattcctctt
     1621 gtactatctt gtctatatta ttctttacag taacccatac ttcattcttg tgctttacga
     1681 tgcttttaag ttcctgcttt gtgcccaaag caattacttt gccatagtcg ataatggcaa
     1741 ttcttgaaca caattcttca acctcttcca tatagtggct ggtatatata acggtgcagc
     1801 cgcgtctgtt caattccttt acggactgaa gaatgtggtt tcttgactgg gggtcaatgc
     1861 ctaccgtagg ttcgtccatt atgattatct caggattgtg ggcaatggca catgcgatgt
     1921 tgagtcttct tttcatgccg cctgagaatc cttttggaaa atccttggcc ttgtcggaaa
     1981 gtcctgtaaa ctcaagggct tccattgcct tgctttttag ttccttgcct cttaaaccat
     2041 aaagtcctgc aaagaatttg acgttttcca aacaagtcag atcctcatat atcgctatat
     2101 cctgagggac tatgccagtg aatgattttg ttaggagagg gtttgatttt gtatttgtat
     2161 ctttaatata tatatttcct tcgtcaattg taatcaagga agtaatcata ttaatcaagg
     2221 tgctttttcc agctccattt ggtccaagaa gaccaaatat ctcaccttca tttattgaaa
     2281 gggacaaatc atttactgcc actatatttc catacttctt ggtcaaattt tcaattctaa
     2341 caatactcaa ttgaataacc tcctgtgtat ctttcgaaac catattatat acttatttta
     2401 cagtgaatgg aatagacaaa tattaacttg ttgaaaaaaa taagaaaatt gtataagata
     2461 ttcctaggat aacgatattt cgttgggagt gaaagagaat gaaaaagcaa ttcattattg
     2521 tagtacttct tctcgtcctc tgcattttcg tcagtgcata tacatataca actgacggca
     2581 gctttgagga tgaagaggtt ggttcgtctt atgtagacct tgacaagttc agctatgact
     2641 ggggtcattc agttgttcat ggcgaaacgg ttattcagga agaggacgga aacaagtatt
     2701 taaaagttac aggattctcg gaattttatt cctacgaccc aataactgca ccttacacat
     2761 ttagtttgga tttgaaactg gaagcggtgg gagatgtgaa tgtttttgtc agggcaggca
     2821 ggaatgaaat tgccccattt cctttctatg agtgggattg gtacgaggaa aagggcggaa
     2881 cgaacggaaa gtcatccact ggaggaccgg gacttttggt ttctcttttg acggattcag
     2941 tacgaatcag gataaagaac atgcagatgg attcggagaa tgaacagatt agttcggtgt
     3001 atcatgattt tccaatgcct gagacgtaca attacaaaga gtttaataat atcaaatttg
     3061 tggatgatgg tgagaaaatt gagatttttg tcaataatga attgattgct acctgtgata
     3121 tgtcagacaa aggcaggtat gaagtcgatg cggcccatcc gccagttgat tttgagtatt
     3181 ttaaaaaggc tgttttgaaa gacagcgagg gtaatgtggt gttggagctg gacaatgcaa
     3241 gacttgtggc tgaagaatgc cgtgtggcct ttggcggaag gagtcatccg ttttatgttg
     3301 acaatgttgc catgagctat gaagtggagg acacaccggc acctacggat actccgaaac
     3361 cagagcctac tgatacggaa gttccaaaag aaacaaatga atcaaccaag aagccggacg
     3421 aagaaaaagg cgagaaggct gattggatac cttttgcggt tgtatgcgtt gtattggtat
     3481 tggtaggtgt tgtaatagca attattgcga gaaggagagc ggtcaagtga atatgagatt
     3541 ttcaggaaaa gcgtttgcat ttgctctaag ttcttttgta atgctaatgt gtactctttc
     3601 aggctgcaca aaaacctatg acattcctga tgctcctgaa ggaatcgtat atcttgttta
     3661 tgaaggaaaa agcaattaca aaatagttgt tggagcaaag gcaagcaagg cagagcagta
     3721 tgcagcggag gaattacaga agtatattaa gaaaattacg gggtttaata ttccgattat
     3781 taaggataat aaaaaggaaa ctgaattgga gattgttgtt ggcaggacca acagggaaaa
     3841 ggattatgaa gtggatcggg actctctggg agaagagggt tttaccatcc tttggagcaa
     3901 caagaagctt gtcatagctg gcggtgggga cagaggaacc atttacggtg tatatgactt
     3961 tttggagtct ttaggttgca ggttctttgc tgaaggtgtg gaaaaagtgc cttcaaacaa
     4021 gaccttggag ctgcagcttc atgaaccaaa gtccgacaag cctgtctttg agtacaggga
     4081 tttgttctgg acaaatacat atgatcctgt atttagtacg aagctcagac tgaacggctg
     4141 tcttaagagc ggagagaatg gaagaaccat acccaagaat ttggggagcg gtatctttta
     4201 tgcaggtcct cactttgtac atacattcga gcatctggtg ccagcatcag tatactttga
     4261 cgagcatcca gagtacttct ctgagataaa cggtgaaagg accgctaagc atctatacag
     4321 tcagctttgt cttaccaatc ccgatgtcct caagatttgt atagacaaag taaaggagtg
     4381 gctcagagag aatcctgaag caaagattgt atctgtttcc cagaatgaca gttttgtgat
     4441 tgaatcgtat tgtacctgca gcgaatgcaa aaaggtggat agggaggagc gttccaagtc
     4501 tggtacattg ataaggtttg taaacaaaat tgcagaggct ataaaagatg agtttcctga
     4561 tgtttatgtg gatactctgg cataccaata ttccatagag ccgcccaggg tgacaaagcc
     4621 tttggacaat gttattgtac gggtgtgcac gggagggtgc tcggctcatc ccattggcaa
     4681 ttgccctaac aattcaggaa taaagggttg tattgaaagg tgggcaaaaa tctcaaacag
     4741 gatatacata tgggactata ctacaaactt tgcccaatac ctctgtccat ttccgaacct
     4801 tgacaccctg cagccaaaca tgcagttctt ctttgagaac aatgttaagg gagtgtttga
     4861 gcagggcaac tatcaggagg gattgaatgg agaatttggc gagcttaggt catatattct
     4921 ggctaaatta ttgtgggatc catatacgga tgtggaaatt catattaacg agtttcttga
     4981 gacgtattac ggaaaggcaa gtccgtatgt gaaagagtat gttgagttcc tgcatgaaaa
     5041 ggtatcagcc ccaagtgttc atttcaacct ggtggttgat gctgcttcct tgtataggtc
     5101 gttgataaac aacgaagagc ttgcacatct tgattctctt tgggaaaagg caaaagagga
     5161 agctgagaat gagagggttt tggaacgtgt aagaagatcg gaactctctt acagattcta
     5221 taaactaact tcaagaagag gagagtttgc ggatgttttc gagtatgaca gacttgagaa
     5281 ggagttttac aaggattgta aagagctggg agttctaaga ttaagcgagg gtgcaaacat
     5341 cccgcttgtg aatccgtaaa ataagccctg caaggtttgg tcctgcaggg cattttgcta
     5401 cttctttgtg tttttgttgc tttcccattc aaaatagccg tccggtataa ttataaccaa
     5461 atcggtttct tttggctcaa ctttggcgag cttgttgttc ttgaacaggt atgtttcttt
     5521 gctgttttcc tccctgtaca tgaaaccatc gataaatccg tatctgagtg caagatacgc
     5581 ctcggtaatt ttctcataca ggtaagggtc ttcaccttcg gttccttggt cgcaattttc
     5641 attgcttggc ggcctgatgg gaaccgtggc ctcccaacct gtttcctctg cgtggtcatt
     5701 gtagcttggg attattatca tttccgggtt ctgttttatt gcattcagcc aggattgtat
     5761 atagtgtttt ccgttctccc tgtaaatggt ttgaatctct attccctgat gagccttgtt
     5821 gtagccaggc tgaacgccaa taacctcgct gccctcattc tgtatgtcaa agacccatcc
     5881 ccagttgcct tctgtttgga atattgactg gtcttgagct ttcttctgcc aggagatgaa
     5941 tcccgtgccg tgtcttatgg taaacctgtc atctgaatga cgtttgttga cttcgccggc
     6001 aatgtacatg ataaggaagg gttttccttt gtactggaag aaggtgccgg ggtgatcgtt
     6061 atgcagtttc caatatacat caagttcctt ttcctgaatt ttcgtattgc catctctcaa
     6121 gggcccgcct gttgctattg caatctgtgg actgttttca ccaagttcgt tggcaacttt
     6181 gaagacttgc tcaatgttct tggcgataag gccgaaatcg ttccagtggc cgtttgtgtc
     6241 gtcaatcatc agatagtcaa cacctatgta tttaagtcgt gaaaggacgc ttgttagata
     6301 atcctcatcg ccacttgaat aatatcctta atcaacgggc tttaaacggg tccagccttc
     6361 agcaaaccag tgatcatggg tcggtttgct ttcgtcagtc caccatactg taaaccaggt
     6421 gcctaccttg gaggaatagt attcctcccc atctttgtaa ggccattgcc caaacttgtc
     6481 atacgctggc acgtctacat atttgttgtc aggttttatg acgtccggtg tagggttggg
     6541 caccctttca accctttctt tttcggtaac acttgtattg ctgcaggatg cggcaaataa
     6601 tgaaaaggtt actacaagta ttatcagttt tttcattttg ctattactcc ttgctaaatt
     6661 ctatttattg tgtaaactgc ccgcccaggc ggcaggcagt ttggtacaag ttaaaatgtc
     6721 tcagccagtc ttattgcttc cttgatgcct tctttaatat tggcctcaac atccgttcca
     6781 ataacatcca gctcatctac ggacaaggtc ttgaaatcca ctattccaag gaatccaaaa
     6841 agtgtcctta gataactgtc tcccatttca tattgcgcag cacctttttc tgtatagttg
     6901 ccgcctcttg ttgtaatatt tatggctttc tttcctttgc acaaaccttc tgcgccatac
     6961 tcggtatatc tgaatgtaat gccacttacc gtaacatagt caagatatgc cttcaaaatg
     7021 gctggaattg aaagattcca caaaggttcg gctattacat atttatcagc gtccctgaac
     7081 tgatatgcgt acttcaggat tgggtgttcc cggtcttctg tctctttgtg ggcaaggact
     7141 gcgttaaggt cgtcttcacg caaaaaatct attccttcct tgtacagatc cagggtgatg
     7201 atttcatcgt ttggatgaaa gctcttgtat gcttcgataa aactgtctgc aattctgaag
     7261 gttctcgatt cgccttcctt ctttgcgttt gccttgatat acaatacacg actcatttat
     7321 attcctcctt ttatgtatat tatgcctttg attaaaaagc atattcagga ctgtgggttt
     7381 ttatacaggt gtggaactgt agtggaataa tacttaaatt atattgtaat gtgaaaaaat
     7441 tatcaacaac attagtctgt gaatataaca caatattctt gtgaggattg tggtatttgg
     7501 ttattcgagg gtgtttgtcg gaaaaagtta ataaaaacca ccgtaatata ccaagcagat
     7561 atttttattt ttgataatat ttacatatgg actaattttt aaagatgtgt ataatataag
     7621 tatgaaaata aacaatgtat tggagatgag aaatagtcat aacaggttgg taataattat
     7681 gaagtgaata ggttttgatt ataaataata taaattaaat atagacatgc tttgcaatat
     7741 atagtaatat atatttacat agtttatcga aaaattaaaa ggaggaaaac aaatgttaaa
     7801 aaaatgtact gtgttggtaa ttgctttgtg catggtatta gcattggttc cgcttaggac
     7861 acaggctgct gatccaccac attcaaagtc catgattttc aaagataatc aggttattaa
     7921 cggctctgtg gctgcgagtg gaaacaatat gaagtcattg gtatccggtg acgtgttgaa
     7981 actcaagata ttgggcaagg gcgaccctaa tattggtatt gattttgtcg agaatattga
     8041 tacctctaca tacaagattc ttgccatgaa ggtcaagaaa gagtatgctg gaggcggttt
     8101 ggaaggcgaa attttctata atgtaaaggg tcaaggtgcc gtaggtggaa aatccattat
     8161 attcacatgg gctgaaggga ccgattggca gtggatttat gttgacctgg gtgctgctaa
     8221 cgcaggggat gttggtttta tcaggttcga cccatacggt gagagtcctg tagaaaatac
     8281 catgataacc gttgctggta ttggattctt caagactgtt gatgacatga atgcatttgc
     8341 tgccacacca gacggacaaa acctcggctc caacgaagga gacagggatt tccctgtaga
     8401 agtacctttt tcaggaactg accttaatat cggtatttgg ctaaatactg tttccgatcc
     8461 ttcgaaacca ggtcactact ggattagatt caatgcagct gctccattct atggaattaa
     8521 cttctacctg tatgcaagca agaacaatgc aaagggtcac tgggcgctgt acaagtttga
     8581 tacatctgtt gaacagactt tggcaggcga gcctgttgca agagaggatt tgtcgctttc
     8641 tcaagacact catttgcaag cagcgttcga gccaatgaat ccaggacagt atatattcta
     8701 tgttgaactg gaaccagacc aggatccaga cccaacagta ttctactatg ttgttgcttg
     8761 ttccagcata gatgcaccag aagggcttat tgagtatggt tttaatggaa caaaacatcc
     8821 agactataat ggtccactgg taatggcagg aactgtaatg tttgttgaaa cagaaggtgt
     8881 taatgactat ctgattccac ttgatgctga aaacccaaca acaggcgatg ttggtatcat
     8941 agcatacgga tttgcagctc ttgcagcagt tctggtaaag agaagaaaag ttaaataatt
     9001 tgtgatgcat aaaaaaggct ggcgtcatgc cagtcttttt ttggtttgcg gggcatgctg
     9061 cccagatgcc actatgtcaa gttgggacga ttggttcgta aaaaaaggaa aaggattctg
     9121 agctataaat cacctttttt agagaaacgt aggttgtgtc ttgtcaatta gtgtaaagta
     9181 tttttctcta tcagtatagc tcttcctaaa cgaattacag aggctctatc caaagaaagc
     9241 aaaattggct gttatgcata gctgcccggt tggataaaat ttctttttat atccttaatg
     9301 gacaatttta cgaatgtata gtaaaatcat tttactaatc aatggaagga ggttatgcta
     9361 cacgtctgta gctgacagta tgcgtagtct tggataagga tatggacaat atgaaatacg
     9421 ctggtttggc aaaagagata gaagagctat tagttgcgtt atcagaacat tacagtaagc
     9481 cctgtggggg agagagtcag gagaagaagg tttatgacaa accggatatg cttgaagtga
     9541 acaaagtcca gtacaacaaa tttaagacta taatttcttt tattcaaatt atttcagaca
     9601 atgataatga tattattcaa taccagtttg gcaaggacgg acttgatgtg aatgtggaat
     9661 ggaaaacccc atcactttac ctgtcaagtt acgaccgaac cattgaactg ttttccagag
     9721 ttcttttgtt ggcagattct gtcaaagttt gtattactgg attggatgag atgtctgtct
     9781 gttttaaaat ttgcaatgta atgcgaaagg tagagaagtc tgaagaatga aagagttgga
     9841 gtacaaattt ttagtagaca aggagttttt caacaaagcg caggaaactc tgaaacggaa
     9901 gtacaagaaa atctctgtta atgtaataga gcaggcaaat tactattatg ataacgacaa
     9961 ctttgagttg cgaaaaagcg gaattactat tagaatacgg cacaaggacg gtgctttgaa
    10021 gttgcaggta aaaaagcatt taaagaccga caagagatat tccctgtgtt acgaagaaga
    10081 gagaagtgtt gatatggttt acggctccat aactgtagag gggaagaggt acgatcttaa
    10141 gggtgtactt cacacaagga ggacaagata tgtcattgac gacggtataa gggttgaatt
    10201 tgacattagt agttatctgg ggcatgagga ttatgaaatt gagattgagt tcttaatgaa
    10261 aagaattgat gatgtcaaga agatgattga aatgcttgac cttccattga ataagagggg
    10321 gaaaggaaaa gcttctcgat tctttgaagc attaaagagc atgagagata agaattatga
    10381 tatgacaaaa ccgaaggaaa tttgaaatcc ttcggttttg ctaatgggca atttgggtta
    10441 aaatggaaat ggttttgagt gttttatctt ttgtatatta attacccgta cattatatat
    10501 ttcgccaatt ctattcaaag aatcggggta gcttgagggc cttttcacga aaaggcttct
    10561 ttttgtatat attatgagta tagtagaata aggtatgaga agtgagagaa tgacggacga
    10621 ggaattggaa aggcagttaa gattcatact aaaacagatt aatgaaaacg taaatgtatt
    10681 ggaagattat atagatgaaa taaaggatga tgaggaactg gcagagttat ttactgccgt
    10741 gagtggaaaa acgctggagg agatgataga ggaacagaat gagtcggaca gaagaagaac
    10801 tgaagagtca agaggttatt atgaaaagtt gaagcagtac atggatgaga aaaactggac
    10861 gtacaggtct attgatgatg atatgattct tgtggtttgc tttgggcttt caaatgttgt
    10921 tgtaaatgtt tttgtaatta tcgagcctat ttcacagacg gtaacaataa atacagtgtt
    10981 gccatatgaa ataaaagatg ggaatgaaga tgcgttgaaa ataatgctgg cagagttcaa
    11041 ctctacttgc aggttcggct cttttgtttt tgatgatgag actggctgtt tggcctacta
    11101 ttactcctgg tgttttttta gacaaaattt tgtaaaggaa gtttttgaga attaccttta
    11161 tgcttgcctg ataccgccgg atgattacgc agaaataatt aatgggatta tttgaaatgt
    11221 ttttgtggta tcaaatagct gttattgaga ataatatatt acggggtgtt ctaaatgttt
    11281 tttggcatgt tttcaggaat tgacgagctg cttgctcagg atacgtcaag ctatcaactt
    11341 tttcattcgc ttccgaaaga gatgcaggag aaaatagttg agcatgactt cacctccttt
    11401 tatgaactaa acaggtttgt tcagaagaac agacacaaat tcaagggtta gcagtcgcat
    11461 gcgcattggt acttgccagt gcgcatgttt ttattgccgt gcatgaatat aagttgtata
    11521 cgagcttctt atagtttctg ataattctat tactaaagtg ttgccatact attaagaatg
    11581 tgatatagtc tataataata tcattaaaaa aacgaaggaa tcaagatgaa aattacgaag
    11641 aagattgtac gggcggtctt tattccgatt ttgttgatgt tgtcagcagt actgatatta
    11701 ataatattgt tatcgatgca cgcagtgaac caacagcgcc agaagcaaaa cagcgccatt
    11761 ggcaatgcaa gggattttat ggagagagca atgcaacact tataccataa tgcggttgtt
    11821 ctttccacaa atggaaatgc ctatggacat gtttcgggtg gtgatgataa tattattgag
    11881 gttattgaag aaagtggagc ggactgtgtc tgttttgtat cgagcacaaa tgtgtatgag
    11941 cgatattttg atactagtgt tgaagatttg ggcaatgatg aaaacgttaa gagtttcatg
    12001 cgatccgaaa aggataccga cataaaattc ataagaggca aaccatttat tcttgcaaag
    12061 gcaagttacg aggataacca atttgattac atattactgg caagagagat ggacaaatta
    12121 ttcttaaatg aacttgacag gataattgat ggaaaaacga cagtaatacc atctgcagaa
    12181 aatgatagtg aaaaatatat actggagctt tctgaggcgg ccgctcttaa aatcgatatg
    12241 gataatggca gggagctgca agttgcaata attgctcttt cagtgtttgt tgtattggtt
    12301 gtgccattaa gtgttgtgct tctttggtat gtaaacaggt acattaataa accgatattt
    12361 gcactgacag acaagataag ggggatttca tattcggata aaggtgaaac ttatgaagag
    12421 acatactgtg atgatgagga gtttctggaa cttatatata caatagacag tctgattaca
    12481 acaaactcta atatattgga caggttgaga aaaggcgatg cggaactgag gcttattctg
    12541 gacactgttg ttgaatgcat cctgggcctt gatttggaag gcaacataac ttattgcaac
    12601 aagcgctgtt tgaacatgct gggatatgac tgttttactg aaatcttggg taggaatata
    12661 aaggagataa caggcaagga tattcaattt tatgaaaaca accagccaaa tatatgccgc
    12721 tttagcggca aagatgagac attggaagtg gaactttacc gttatcctaa aattgaagat
    12781 aacaggattg ttggatacgt tgttgcgatt attgatatta cagaaaagag gaaaatggaa
    12841 cacgagctta ctgaaagcca aagaagcacc gaattccttt tgacgaacat aatggggatg
    12901 gtatatagat gcagacatga tgaaaactgg accgtgctat atgtatcgga agggtgttac
    12961 aagctcacag gatacaaggc tgaaagtttt attaacaaca gagatgtcac tttgaacgat
    13021 attattgcta aacccttccg ggagagaatc aggcagcggt atgaaattgt acttgccaac
    13081 agggaagtgt tccgcgagga gtacataatt gtaacagcgg atggtagtga aaaatgggtg
    13141 ctggaacagg gacagggagt ttatgacgat gaaggcaatg tagttgccat tgagggattg
    13201 ataatagata tatctgatca aaaagcaaga gaaaaggaaa ttgagtatct cagctatcat
    13261 gatgcgttga caggagtgta caaccgtatc tatttggata gggaaaagaa gagattgatg
    13321 gaatcggaca tgcatccgat ttctgtcata gttggggata tagacggtct aaaatttatt
    13381 aatgatgcat ttggacatgc tgtaggagac gagctaatta aacgtacagc aaacatactg
    13441 aaaagctgtc tgagagaagg agatgtcata gcgagggttg gaggagatga attcaacatc
    13501 cttcttccca aaacggatga atctgaagca cacaggataa tgaagaatat gcagagggaa
    13561 ataaagaaat tcagtagtga gaacatgagg ctgtacaatt ttactgtgtc gctgggttat
    13621 tcgaccaagt ataccaggga agataatcta aacgattgca taaagcaggc tgacgagtac
    13681 atgtataaaa gaaaactttt ggagcgccac agctctcaca gtgcagttat tgcatccatg
    13741 agggaaacca tgaatgcaag gggtatagaa acagaagagc actgcagaag gcttgctgat
    13801 ttgtcaagag aaatgggtat ggctatggat ctttctgaaa aggaagtgaa tgagcttgtg
    13861 cttttatcat cattgcatga tattggcaag gtgggtatta atgatgcaat agtaaaaaag
    13921 gcaacaacca tgaccaatga agaatttgaa gagatgaaaa aacatcccat gattggatac
    13981 agaattgcca tggcaaccca cgagctgcag tcgattgcgc catacattct tacacaccac
    14041 gaaagatggg acgggctagg ctatccccag ggacttaaag gcgaagaaat acccaaagcc
    14101 tccagaatat tttcagtact tgtcgcatac gatgtgctga cctgcggaag accttataaa
    14161 cagcctgtct caaaagaaga ggcattgaaa gaaatagaag aaaatgcggg acttcagttc
    14221 gatcccgcaa tagcaaaaat atttgttgat tggatgagaa aacaacatta gaaaagaccg
    14281 aaaatagttc catcttcctt aatatcaaga tatgctgctg cagggttttt aggaagtcct
    14341 ggcattgtca atatgttacc agtttgcaca acaataaaac ctgcaccggc agacactcta
    14401 acatctgaaa ccgttatttc aaaattttta ggtcttgtca acagttttgg attgtccgac
    14461 aatgaatact gggtcttggc catgcataca ggcaggtttg aatagcccag ttcttcaatt
    14521 gtgtttattt cttcttgagc ctttggtgtg tatactacac cttttgctcc atatatttta
    14581 ctgcatatgg tttctatttt ctcttttatt gaaatattat cgtcatacag gagcttgaat
    14641 ttgctattgg tttcagcaag ttctacaatt ttttcagcaa gttttattcc gccttttccg
    14701 ccatctgcat gcaatgtgca tatttctgca tccactccca gattgctgca atagttaaca
    14761 atgcttcttg tgtcatcttc gctgtcgttt gcaaagtgat ttattgcaac aattgctgga
    14821 actccaaagc actgaatgtt ctctatgtgt ttgccgagat tatcaaggcc tttttcaatg
    14881 tcgccgtttc catggtgctt gagtgctctt accgttgcaa ctataactgc ggcggaaggt
    14941 tttaggtttg ctttcctgca cttaatattg aagaatttct cagctccaag atctgctccg
    15001 aaccctgctt cagttataac gtagtctgcg gttttaagac ccagcttggt tgctatgacg
    15061 ctgttgcagc catgggcaat attggcaaaa g
//
LOCUS       DAONVY010000002        14838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_3030_length_14838_cov_3.540283, whole genome shotgun sequence.
ACCESSION   DAONVY010000002 DAONVY010000000
VERSION     DAONVY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3030_length_14838_cov_3.540283"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1002)
                     /locus_tag="PLI11_00080"
     CDS             complement(184..1002)
                     /locus_tag="PLI11_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51297.1"
                     /translation="MEVEYTYLNIGGYCIKADLDMFTPHALAFKSFVCDKKDSDFELE
                     VVFDNNGITIEGDKVVDVTEDGYKIELKNQNVLSASKDWRKWSIYAGKKNDRIDHVIY
                     YELKKPFSYAILPYGGVVFHGVVLEWNGKGIILCAESGGGKTTHARMWRDYENALVIN
                     GDRALCRKVEGVWHAFGQPWCGTSGECVNRKIKIDVFVMLKKSMQNRVTRLNPYDAFL
                     NLAEHVFAPKWNAELMNYALDYIDDMVTNIPMLKLECVKDYTAVETLKKAILDL"
     gene            complement(1008..1322)
                     /locus_tag="PLI11_00085"
     CDS             complement(1008..1322)
                     /locus_tag="PLI11_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51298.1"
                     /translation="MKMYEKPKLRFEEICLGESIADTCFGYKHFNFKGTLKVDNKSYN
                     VSFNSGTLHDCSAAAVFAAIDDFLDRYLNKDITFDPYANGMKDNIGNTKHPGFYFGEL
                     QS"
     gene            complement(1309..2364)
                     /locus_tag="PLI11_00090"
     CDS             complement(1309..2364)
                     /locus_tag="PLI11_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPZ51299.1"
                     /translation="MHEERIPLNGTFELTARCNLKCKMCLVRVDEETISSLGLRELTT
                     KEWIDLAKQARESGTLQLLLTGGEPMLRKDFCKIYTEMYKMGFVIVLYTNATLVSDKV
                     MDVLEKCPPHQIGISVYGSNPSIYEKVCGSGNAFYRMVDGVKKLTTLPSSINIRTTII
                     KDNIGDYKNIYMLAKSFGEDITFSRSRIVCRTTRHGIADVDACRLSPEENIDFIYGAY
                     LERLSKDLIDKYTVEEKKSELFGCDAGIQSFTITWDGKLIGCQLLGEFMTDPLSEGFE
                     KVWETFVETVPELKQIDTCRDCKYKLFCMACPALRYCETKSLFGNPKYLCREAEYSHK
                     ILEKLISTKGSEIYENV"
     gene            complement(2366..2638)
                     /locus_tag="PLI11_00095"
     CDS             complement(2366..2638)
                     /locus_tag="PLI11_00095"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPZ51300.1"
                     /translation="MRVNQNFVLRKIKGANIIVPLGEAIKKANGIMVLNETGAYIFEA
                     LQEEKDFEELVSCIMVEYGVPLDVAKSDTSDFLEELRTYGILMEEE"
     gene            complement(2652..2918)
                     /locus_tag="PLI11_00100"
     CDS             complement(2652..2918)
                     /locus_tag="PLI11_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPZ51301.1"
                     /translation="MKVRGNYIMRKIAGTNVIIPVEDSVADLDGVIAVNNTAAFLFSK
                     LIEEITFDELVDGLINEYDVTKEEASQDVQAFISILKERKMLEE"
     gene            complement(2941..3633)
                     /locus_tag="PLI11_00105"
     CDS             complement(2941..3633)
                     /locus_tag="PLI11_00105"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HPZ51302.1"
                     /translation="MKYSGKEVFNIMEGVMALDIDGKTDITDQVNVIIVTDTSIGKKI
                     IRYSVVGSNGNLATNERRLNLINYTGPSIHVSNDVVITSEDLDDLVNILIERDAIRAD
                     DGFGNDITRTISYNYEAFADSHKCKFTFTVQNVFNDVASKSIFVEVEGSSAGPTLIIA
                     RKTVALKKGDVFNPYEYIKSAYDKKDGDLTEFVVLSGKVDTNKEGQYTVTYTLSNLEG
                     VSVTQRLVVYVG"
     gene            complement(3838..5049)
                     /locus_tag="PLI11_00110"
     CDS             complement(3838..5049)
                     /locus_tag="PLI11_00110"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPZ51303.1"
                     /translation="MNPTCKAFLKLIKSHIRNEEPNMDSDVNLEEVFSLACKHNLLPL
                     VYDAASLLEYEGKERVRNHALYTMIGQTKRTFEFLKVYNFLVHNGLKPLVLKGLVARS
                     LYQSPDLRISSDEDIYIRKEDYALCHDLLMRYGYRVGKTCANKDPKDAQAISYMAPNS
                     DLCIEVHVNLFGLSGVRKELNKYFNESFDRCITLEIEGFEIYTLCHTDHFLFIFLHLY
                     KHLIGYGVGIRQCMDLVLFAKKFFEQICWEQIIAIVRELGCEQLLYSLFDIGINELGI
                     CLDISDIPFDGNIKVSNRMTLIVDMIEGGVFGKSCSERIFSGPVVQMAASSKKRMNVI
                     LGFVKACFPSKEYLKYSYPVLKKHSFLLPFVWVYRIIRYLKRTKEPAVVEGLGIARKR
                     LSLLEEYGLVQ"
     gene            complement(5012..>6754)
                     /locus_tag="PLI11_00115"
     CDS             complement(5012..>6754)
                     /locus_tag="PLI11_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51304.1"
                     /translation="DGSLKEMWMEAKWIYGYIKRYKMQVIFYIFLFSVSTMFGLLNAV
                     ASKFLIDSLANRQLKVIITIAIIYISFGLFEILTGALTNRITSKIDLRIINDIRIDIY
                     DSILNIDSEHLMDFHSGDLLNRINSDARAVAGSVVGMIPSFVTRVLQFSGSFIIILYF
                     DHVMALLALASAPVSMVVSDLLVRKMRKHSKRMREASSKLTSYHEESFVNIAAIKALG
                     LKSVFSNRHKEQLEQYTTTSLDYNKFSIHTHLFMSITGLVVSYLCFGWAVFRLYQGFI
                     TFGTMVLFLQLAGMLRSSFSSLISFIPSAISATTSAGRVMAIIEKPTDACDISNDEQI
                     LKSARKRGVAISLEGVSFSYRRGKKVFEDASILARPGETIALVGPSGEGKTTILKILL
                     GLLKPQKGRLNIYPYNEKHKNIVFDDKARILFSYVPQDNMLFSGTIAENLRLAKPDAT
                     EEEIKEALVMADAYDFVSKLPEGIYSNVEERGKNFSEGQIQRLSIARALIRKAPILLL
                     DEATSSLDVDTERRILMNIKRIKGEVTCILTTHRPSVLGICDRVYRVSGGRVALFGGG
                     ESLEPDLQGVLEAY"
     assembly_gap    6755..6854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6929..8356)
                     /locus_tag="PLI11_00120"
     CDS             complement(6929..8356)
                     /locus_tag="PLI11_00120"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPZ51305.1"
                     /translation="MDNETSIDIMVLLSDLIKGIKKFWHIVLVLASVMSWLFFYVSAR
                     KYTPLYTSKATFTILAAYVDNYAGYSYRYYYNSQTASQMAVTFPYILNSYLLQEIVCE
                     DLGVPYLNGSINAVAIPDTNLFTIMVTSTNPNDAYHILNSVIRNYPKVAEFVIGDTKM
                     NMLSSPKVPTNPTNRPVYSRMTKIGFLAGCVLGCGFIFLYALTRTTIRKVDDVKKILN
                     LECLGVLPVVKFKRRKNDMDHSVNIMNDKVGSAFGESIRSIRTKLLKETSDKKNNVIV
                     VAGSVPKEGKTTLAINLSIALGQMGYSVILVDGDLRNPSVLNMLDIVMPLNGLGEILE
                     GEQTIEQSIHGFGKYNISVLAGQKSYPNPNKLLSHHSLVEVIIMIRNMADFVIIDTPP
                     CHLVPDAVAFVKLADHAVYCIRQDYAKRSLIIDSLTSLSYSQVNVLGAVLTFTETGIG
                     SYGYRYGYLYKYPYYRKLRYYGESV"
     gene            complement(8349..8564)
                     /locus_tag="PLI11_00125"
     CDS             complement(8349..8564)
                     /locus_tag="PLI11_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51306.1"
                     /translation="MVKQDFARVREINDAIDKMDKSKCQLLGIVLNNLKGFPFASSVR
                     SNMYKSYYYRRVPLFKKKEDTKEVSHG"
     gene            complement(8524..9408)
                     /locus_tag="PLI11_00130"
     CDS             complement(8524..9408)
                     /locus_tag="PLI11_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPZ51307.1"
                     /translation="MMEKEFSIEDIDIYTLLRDILLNLWTIALGGAVVAMLAIFYVRY
                     TYVPEYRSQVTFVVSTKDATSSMYANLSTTIEMANIFTQIMDSDILKEKASQIAGYEE
                     LPGKIDAYAIPETNLLVVGVTAKSPEDAFKTLKLIIENYRLVSDYIYGNAHLEDIEDI
                     EMSDWPVNSVDEPRIRKLSFLAGAFLSACAVCALSFFRDTVKNEKQANSKLDAKLLST
                     IRHERKYITLRQVLKRAKRTPLVIDMFASIDYICSYKKLYRLAAAWCGVRRRNPCKHS
                     RFFNTCGKTGLCKSKRNQ"
     gene            complement(9405..10229)
                     /locus_tag="PLI11_00135"
     CDS             complement(9405..10229)
                     /locus_tag="PLI11_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51308.1"
                     /translation="MKIFRAVILLLSIVTGIFYFIQFGVYGVKTNGTYPTITTDRKVI
                     EVSIMDKDKLFEGISARDDQDGDLTGKILIESISKFIEKGVCNITYVVCDSDNNVSKL
                     TRKVKYTDYESPKFIIDHEPQFYIGSTISMDDFIKCYCLIDGDISSKIKYDFGNLTTN
                     VPGVYTVKAMVTNSKGDVVDLSFEVTVRERNVKTPKIKLESYVLYVKTGEEVDPKDYI
                     QSVTGIDGMLLDISLVNIYSDLDTSKPGEYRIYYSVTEGVETYTTSMVCVVEEGNA"
     gene            complement(10226..10957)
                     /locus_tag="PLI11_00140"
     CDS             complement(10226..10957)
                     /locus_tag="PLI11_00140"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51309.1"
                     /translation="MIDIHAHLIPGVDDGSVSFFESVRMLEMACQRGVRALVATPHSN
                     IPIQSNYYGEWFENCFKKLQELTKEIPITLYKGMEIYLTKDVVDLVKDNKLITINNTR
                     YILIELPVFDSTHNILTNIKSIINANLIPIIAHPERYELFHDDTSLLMQLASQGCIFQ
                     INKGSILGQFGATSQKLAKYMLKHGLVHVIASDAHSPYQRTTRLDEAYDYICYRYGTD
                     YAKTLFYINPGRILKDENIDRWGLL"
     gene            complement(11160..11630)
                     /locus_tag="PLI11_00145"
                     /pseudo
     CDS             complement(11160..11630)
                     /locus_tag="PLI11_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014485528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
     gene            complement(11698..12687)
                     /locus_tag="PLI11_00150"
     CDS             complement(11698..12687)
                     /locus_tag="PLI11_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51310.1"
                     /translation="MFVDASVQEWTELYEVAKVTKSLKPWQYVGDDWILALDIPGRDE
                     PCYCIVTGEDSDAIAIAIYPNRTSLAKYSEYLSNKDDTYQVKCEQNYLICYFCDRQEM
                     LSMQLEKLRELGISFRGKNEWIQFVSIRPGYAPYMLDKGEVLFLKGFFEQFNEFIKDY
                     SKGCMNFSIKDGNVLYRNFVGNNNYSYTQQSLEVSPDVNKIMPIHDEDLVNLVKKMPA
                     TDSEMELDLVYIDSTIYDEDLDRPAIGRLVVLGENLSESILGVKVVLPTQNPAKEIYD
                     FITQKITIHGRPKVIYCKRDICYETLAEFCKKTEIKLERISNLVLIDELVKAI"
     gene            complement(12864..13871)
                     /locus_tag="PLI11_00155"
     CDS             complement(12864..13871)
                     /locus_tag="PLI11_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51311.1"
                     /translation="MIKATLDDWAQLYGHAIRLKNLRPWESLSFLDTISIKMPDRDEM
                     YYVSIDENPELISIHILRDSSSYAKFIKTFLKPDYTHEEIFNDICSVDIFTMCIGSRE
                     SVPKSQYNIIKELGLSFRGRMDWVFFLSKRKCYAPADPDKIEVMLLAECIEQICNAVE
                     DYNTNKIDVRFDDGFALCRYFDDDEKRWMTIEKQVPIVNAQSDYYKYENEIQIQRIKK
                     TPYSGESFDIDITFVQSRIRDTQFERDPIVRLLVISNRNSGDILETRIVLPNDDLAEL
                     IFEAVFSAIGKTGRPAKIHYTKDICEELLDDFCSKTGILLAKEERLPSIEQFLHSMMF
                     D"
     gene            complement(13936..14706)
                     /locus_tag="PLI11_00160"
     CDS             complement(13936..14706)
                     /locus_tag="PLI11_00160"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HPZ51312.1"
                     /translation="MFRLDGKVAWVTGGSYGIGFAIASALSEAGATVVFNGIHEENVK
                     KGIKEYEKHGLNVKGYICDVTKEDEVKEFADMLIKEYGHIDILVNNAGIIKRIPAVDM
                     TADEYKEVIDVDLVAPFIVSKAVTPHMIKNGGGKIINICSLMSELGRETVCAYAAAKG
                     GLKMLTRSLASEWGQYNIQVNGIAPGYIATPQTAPLRQDGNPFNDYIKARTPANRWGT
                     PEDLKGPAVFLASKASDFVNGHVLFVDGGLLAYIGKQP"
     gene            complement(14719..>14838)
                     /locus_tag="PLI11_00165"
     CDS             complement(14719..>14838)
                     /locus_tag="PLI11_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HPZ51313.1"
                     /translation="PSWSIHAGCGTGSYSFIWAMAGENREFTDMDHIPTLELK"
BASE COUNT         4025 a         3368 c         2524 g         4821 t
ORIGIN      
        1 caagcttagc cttgcaaaat ataccgagca gaaccttgtc tttgttgtgc cgaataaatg
       61 gcagcatgct gtttccggac acggtaaact taaccctcaa gttttgcctt agcaaatcct
      121 caacaagagg gaacaattct ttacacgaca ctattctctt atacgtcatt gactttttaa
      181 cacctacaaa tcaagaatgg ccttttttag tgtttcaact gccgtgtaat cctttacgca
      241 ttcaagcttc agcatgggta tgttcgttac catgtcgtca atataatcta gggcatagtt
      301 cataagctcc gcgttccact ttggagcaaa tacatgttct gcaaggttaa gaaatgcatc
      361 atagggattt agtctggtta ccctgttttg catgcttttc ttcagcatga caaatacatc
      421 aatctttatc tttcgattca cacattctcc tgatgtcccg caccaaggct gaccgaaggc
      481 atgccataca ccttcgactt tacggcataa tgctctatcc ccgtttatga ccaatgcgtt
      541 ttcataatct ctccacattc ttgcatgggt ggtctttcca ccacctgact ccgcacatag
      601 gatgattcct tttccgttcc attcaagcac aacaccatga aacacaactc cgccataggg
      661 aaggattgcg taagagaagg gcttttttag ttcgtagtat attacatggt cgatcctgtc
      721 atttttcttg cctgcataga tgctccactt tctccaatcc ttacttgcac ttagaacgtt
      781 ctggttctta agctcaattt tgtaaccatc ttctgttaca tcaaccacct tgtcgccttc
      841 aatagtaata ccattattgt caaaaacaac ttcaagttca aaatcgctat ctttcttgtc
      901 acaaacaaaa gatttgaacg caagggcatg aggtgtaaac atatccaaat cagctttgat
      961 gcaatatccg ccaatgttta aatatgtata ctccacttcc atttcaatca gctctgtaac
     1021 tctccaaaat agaaacctgg atgttttgta ttacctatat tatctttcat cccattagca
     1081 taaggatcaa aggttatgtc tttatttaga tatctgtcga gaaagtcatc tatagctgca
     1141 aatactgcgg cagcactaca atcatgcagt gttccgctat taaaactaac attatatgat
     1201 ttattatcaa cttttagagt ccctttaaag ttaaaatgtt tgtagccaaa acacgtatct
     1261 gcaatgcttt cccctaagca aatttcttca aatcttaact ttggcttttc atacattttc
     1321 atatatttca cttcctttcg ttgagattag cttttctaat atcttatgac tgtattcagc
     1381 ctctcggcat agatattttg gattaccaaa taaactcttt gtttcacagt atctcaaggc
     1441 aggacatgcc atgcaaaaca acttatactt gcaatcgcga catgtgtcta tttgcttcaa
     1501 ttcaggcaca gtttccacaa atgtctccca aaccttttca aaaccttcgc tcaatggatc
     1561 tgtcataaac tctccaagca attgacatcc aattaatttt ccatcccaag taattgtaaa
     1621 tgactggata cctgcgtcgc atccaaataa ttcgctcttc ttttcttcta ctgtatactt
     1681 atcaattaaa tccttcgaca atctctccaa atacgctcca taaataaaat caatattctc
     1741 ctcaggtgac aatctgcatg catccacatc agcaatgcca tggcgtgtgg tcctacaaac
     1801 aatcctgctc ctgctaaaag taatgtcctc tccaaaactc tttgccagca tatatatgtt
     1861 tttgtaatct ccaatgttgt ctttaataat cgttgttcta atattaattg atgaaggcaa
     1921 agtcgttaat ttcttcactc catccaccat tctgtaaaaa gcattgccgc taccgcacac
     1981 cttctcataa atggatggat tggagccata aacagatatt ccaatttgat gaggaggaca
     2041 cttttccaac acatccatta ctttgtcgct gacaagagtt gcattggtgt acaggacaat
     2101 aacaaacccc attttataca tttccgtata aatcttgcaa aagtcctttc gcagcatagg
     2161 ctctccccct gtgagcaaaa gctgcaaagt cccgctttct cttgcctgtt ttgcaagatc
     2221 gatccactct ttggttgtaa gttccctaag acccagactg cttattgttt cctcatccac
     2281 tctgacaagg cacattttgc atttcaagtt gcacctggca gtcagttcaa aggtaccatt
     2341 taaaggtatt ctttcttcgt gcattttatt cctcctccat aagaataccg taagttctga
     2401 gttcctccaa aaagtcacta gtatcacttt tggcaacatc caaaggaacc ccatattcca
     2461 ccattataca tgagaccagc tcttcaaaat ccttctcctc ctggagtgct tcgaatatgt
     2521 atgcgcctgt ttcattcaag accataatac cattggcttt ttttattgcc tctccaagag
     2581 gaactattat atttgcacct tttattttcc tcagtacaaa attttgattg acacgcattt
     2641 acagcacccc gctattcttc cagcattttt ctctctttaa gaattgatat gaatgcctgg
     2701 acatcctgac tagcttcttc ttttgtaacg tcatattcat ttatcagacc gtctaccagt
     2761 tcatcaaatg taatctcttc tattagttta ctgaataaaa acgctgctgt attattaacc
     2821 gcaattacac catccaggtc cgctacacta tcttctaccg gaataataac gttagtacct
     2881 gcgatttttc gcatgatata attcccgcga accttcattg atcctcctcc tttcctagta
     2941 ttagccaaca tagaccacaa ggcgctgtgt gacgctcacc ccttccaaat tgcttaatgt
     3001 ataggtgact gtgtactgac cttccttatt cgtatccact ttgcccgata aaacaacaaa
     3061 ctccgtcaaa tcaccatcct tcttgtcata cgcactcttt atatattcat aaggattaaa
     3121 tacatcccct tttttcaaag ccacggtttt tcttgcaatt atcagtgtag gacctgcact
     3181 gcttccttcc acttcaacaa agatggactt gcttgcaaca tcattaaaca cgttttgaac
     3241 agtgaaagta aatttgcatt tgtgagagtc tgcaaaagct tcatagttgt acgaaatggt
     3301 ccgagtaatg tcatttccaa agccgtcatc cgctcgtatg gcatctcttt caataagaat
     3361 gtttaccaaa tcatcaagat cctcactggt tattaccaca tcattagaaa catgtattga
     3421 cggccctgtg tagtttatga ggttgagtct cctttcgttt gttgccagat ttccatttga
     3481 acctaccaca ctgtacctta taattttctt tccaattgag gtgtcagtaa caataattac
     3541 attgacctgg tcggttatat ccgtcttgcc atcgatatcc aaggccatca cgccttccat
     3601 aatgttaaaa acttctttac ccgagtactt catatctcca tgtgaaagcc tgatggttgg
     3661 tttcttgttt gaatcagcac cactattatt tgaaagactc gctttaataa tatccttgtt
     3721 gattgcatac gctatgtcct gatgaaaaaa tacaaaacaa acaaacttac tatcgatact
     3781 acagtaagca caattatcgt tatattttta acaatcttca ataacatcac ccctacccta
     3841 ttgaacaaga ccgtattctt caagaaggga aagcctcttt cttgcaattc caagaccttc
     3901 aaccactgcc ggttcttttg ttctttttag atacctgata attctgtata cccacacaaa
     3961 aggcagtaaa aaggaatgtt tctttagtac cgggtacgaa tatttgagat attcttttga
     4021 aggaaaacat gccttcacaa atcccaaaat gacattcatt ctctttttgc ttgaagctgc
     4081 catctgtaca accggtcctg agaaaattct ctcgctgcag gacttgccaa acactcctcc
     4141 ttcaatcatg tcaacaatca aagtcatgcg gtttgaaact tttatatttc cgtcaaaagg
     4201 aatatctgaa atatcaagac aaattcccag ctcattaatt ccaatgtcaa acaaggagta
     4261 aagcaactgc tcacatccta attctctgac aattgcaatt atttgttccc agcaaatctg
     4321 ttcaaagaac ttctttgcaa aaagcaccaa gtccatacac tgtcttatgc caacaccata
     4381 acctatcaag tgtttgtata aatgaaggaa gataaacaga aagtggtctg tgtgacagag
     4441 cgtataaatt tcaaatcctt ctatttccag tgttatgcat ctgtcaaagg attcattgaa
     4501 gtatttatta agttcctttc ttacacctga caaaccaaac agattaacat gcacttctat
     4561 gcaaaggtct gaattgggtg ccatgtacga aattgcctgg gcatcctttg gatccttgtt
     4621 tgcacatgtt ttacccactc tgtatccata tctcattaaa aggtcatgac atagagcata
     4681 atcctcctta cgtatgtaaa tatcttcgtc ggaagatatc ctaaggtcgg gagactgata
     4741 caagcttctt gcgacaagcc cttttagcac aaggggtttc agcccgttat gtacaagaaa
     4801 attgtacacc tttaggaatt caaaggttct tttggtctgt cctatcatgg tgtataacgc
     4861 atgattacgc acacgctctt ttccttcata ctcaagcaaa cttgccgcat catacacaag
     4921 aggaaggaga ttgtgtttgc aggcaagaga aaacacctcc tcaaggttca catctgaatc
     4981 catattgggc tcttcatttc ttatgtggct tttaataagc ttcaagaacg ccttgcaggt
     5041 cgggttcaag actttcacct ccgccaaata acgcaactct tccgccactt actcggtaca
     5101 ctctgtcgca aataccaagc acgcttggtc tgtgggttgt aagtatgcag gttacttctc
     5161 cctttatcct ctttatattc atcagaatcc ttctctcggt atccacatca aggctgctgg
     5221 tggcctcatc aagaagcagt atgggcgctt tccttatcaa tgccctggca attgaaagtc
     5281 tttgtatctg cccctcggag aaattctttc ctctttcttc aacatttgaa tatattccct
     5341 caggcagttt tgatacaaaa tcatatgcat ccgccattac cagagcttcc tttatttcct
     5401 cctctgttgc atcgggtttt gccagtctca agttttctgc aattgtccct gagaacaaca
     5461 tattgtcctg aggaacatag gaaaaaagaa ttcttgcctt gtcgtcaaac acaatattct
     5521 tgtgcttctc attatatgga tagatattaa gtcttccttt ttgaggtttg agcagtccaa
     5581 gcaatatctt aaggatggtg gtttttcctt ctcctgaagg acctaccagg gcgattgtct
     5641 ctcccggtct tgcaagaatg cttgcatctt caaacacttt cttgcccctt ctgtagctga
     5701 aggaaacgcc ctcaagactg attgccactc ctctcttccg cgcacttttc agtatctgtt
     5761 catcattgga aatatcgcag gcatctgttg gtttttcaat aattgccatg actcttcctg
     5821 ctgatgtggt tgctgatatg gcagagggta taaaagatat cagggatgaa aagcttgatc
     5881 tgagcatgcc tgcaagttgc agaaacaata ccattgttcc aaaggttatg aacccctggt
     5941 acaatctgaa aaccgcccaa ccaaagcaaa gataagagac aaccagtccg gttattgaca
     6001 tgaacagatg agtatgaatt gaaaacttgt tatagtccaa acttgttgtc gtatattgtt
     6061 caagctgttc tttgtgtctg tttgaaaaaa cagacttcag ccccagagct ttgatggctg
     6121 ctatgttcac aaaagattct tcatgatagg acgtgagttt tgaacttgct tccctcattc
     6181 ttttgctgtg cttcctcatt ttccttacca acagatccga tacaaccatg gatacaggcg
     6241 cactggcaag agcaagcaaa gccataacat ggtcaaaata caggatgatt ataaacgagc
     6301 cggaaaactg caataccctt gttacaaaac ttggtatcat gcctacaaca ctgcccgcaa
     6361 cagcccttgc atcagagttg attctattta gaaggtctcc tgagtgaaag tccattaaat
     6421 gttccgagtc aatattcaat atgctgtcat atatgtctat ccttatgtca tttattatcc
     6481 ttaaatcaat ttttgaagta atcctgttgg taagtgctcc tgtcaatatc tcaaacaaac
     6541 caaaagatat gtatattatt gcaatggtga taataacttt taactgcctg tttgcaaggc
     6601 tgtctattaa aaatttggag gcaactgcat ttagaaggcc aaacatggtg cttacggaaa
     6661 agaggaagat gtaaaagata acttgcattt tgtatctttt tatatacccg tatatccatt
     6721 ttgcctccat ccacatttct ttcaaagatc cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnttatat acccgtatat ccattttgcc tccatccaca tttctttcaa
     6901 agatccgtcc tggatcttcc tctttatact aaacagattc accataatac ctcagctttc
     6961 tgtaataagg atatttgtat aaataaccgt acctgtaacc gtaactgcca atacccgttt
     7021 ctgtgaaggt caaaactgct ccgagcacat tgacctggct gtatgacaac gatgtcaaag
     7081 agtcgattat caagctcctt ttggcataat cctgtcttat gcagtaaacc gcatggtccg
     7141 caagttttac aaaggcaaca gcgtcaggaa ccaggtggca gggcggagta tcaattatta
     7201 caaaatcagc catgtttcta atcataatta ttacttccac aagggaatga tgcgacaaca
     7261 gcttgtttgg atttgggtat gatttttgtc ctgccaatac gctgatgttg tacttgccaa
     7321 aaccatgtat gctctgctct atggtttgtt ctccctctag aatctcccca aggccgttta
     7381 gaggcatgac tatatccaac atgttcagta ccgaaggatt tctcaaatcc ccgtccacaa
     7441 ggattaccga gtaacccatc tgtccaaggg caatactcag gtttatggca agggtcgttt
     7501 ttccctcctt gggaacactg ccagctacga caattacatt gttcttttta tctgacgttt
     7561 cctttagcaa ctttgtccta atcgatcgta tggactcgcc aaatgcactt cccactttat
     7621 cattcataat gtttacggaa tggtccatgt cattttttct tctcttgaat ttcactactg
     7681 gcagaacgcc aagacactca agattaagaa tcttctttac atcatccact tttcttattg
     7741 tggttctggt caatgcataa agaaagataa atccacagcc aagaacacat ccagccagga
     7801 atcctatctt tgtcatgcgt gaataaacag gacggtttgt aggatttgta ggcactttgg
     7861 gagatgacag catattcatc tttgtatctc cgattacaaa ctctgctact ttgggatagt
     7921 tccgtattac agagttcaga atgtggtatg cgtcatttgg attggtgctt gttaccataa
     7981 ttgtaaacag gtttgtgtcg ggtattgcta ctgcgtttat gcttccattc aagtaaggga
     8041 caccaagatc ctcgcacacg atttcctgga gaagataact gtttaatatg tatggaaaag
     8101 taactgccat ctgggaggca gtctggctgt tgtagtagta gcggtagctg tatcctgcgt
     8161 aattgtccac atatgctgca agaattgtaa aagtggcttt tgaggtatac aaaggagtat
     8221 actttcttgc agagacataa aagaacagcc aggacataac gcttgcaagt accagcacaa
     8281 tatgccaaaa cttttttata ccctttatca aatcgcttag aagcaccatt atgtctattg
     8341 aggtttcatt atccatgact aacctccttt gtgtcttctt tctttttgaa cagaggaacc
     8401 cttctgtaat agtagctttt gtacatgttg cttcttacag aactggcaaa aggaaaacct
     8461 tttagattat tcaaaacaat accaagcagc tggcatttgc ttttatccat cttgtcaatt
     8521 gcatcattga tttctcttac tcttgcaaag tcctgtttta ccacaagtat tgaagaatct
     8581 gctgtgtttg caaggatttc tccgtcggac accgcaccaa gcggcggcga gtctatacag
     8641 ctttttgtat gagcagatat aatcgatgct ggcaaacatg tcaattacaa ggggcgttct
     8701 ctttgcccgc tttaaaacct gtctcaaagt tatatacttg cgctcatgcc ttatggtcga
     8761 caatagtttt gcatccagct ttgagtttgc ctgtttttca ttctttactg tatctctaaa
     8821 gaatgagaga gcacaaactg cacaagcaga caaaaaagct cctgccaaaa aggacagttt
     8881 ccttattcta ggctcatcca cgctgtttac cggccagtct gacatttcga tatcttcaat
     8941 gtcttcaaga tgtgcattcc catatatgta gtctgacacc agcctatagt tctcaataat
     9001 gagctttagg gtcttgaaag catcttccgg gcttttggcg gttactccaa ccaccaaaag
     9061 atttgtttcg ggtatggcgt aggcatcaat ttttccaggc agctcctcat acccagctat
     9121 ttgagatgcc ttctccttta gaatatcgct gtccattatc tgggtgaaaa tatttgccat
     9181 ttcaatcgtg gtgctgagat ttgcatacat ggagctggtt gcgtcttttg tacttaccac
     9241 aaaagtaacc tggcttctgt attcaggcac atatgtgtac ctgacataga aaatcgcaag
     9301 cattgccaca actgccccac caagtgcaat tgtccacaga ttcaagagaa tatctctcag
     9361 aagcgtgtat atatctatat cttcaatgct aaattctttc tccatcatgc attcccttct
     9421 tcaacaacac ataccatgct tgttgtatag gtttctacac cctctgtcac actatagtag
     9481 atcctatact ctccaggctt tgatgtgtcc agatcggaat agatgttcac aagggaaatg
     9541 tcaagaagca tgccgtcaat tcctgttact gattgtatgt agtcttttgg gtcaacttcc
     9601 tctcccgttt taacatacag cacatagctt tcaagcttta tctttggtgt ctttacattc
     9661 ctttccctga ctgtcacctc aaagctcaaa tcaaccacat caccttttga gtttgtaacc
     9721 atggctttta ctgtgtacac tccaggtaca tttgttgtca gattgccaaa gtcatacttt
     9781 atcttgctgc ttatgtcccc atcaatgaga caatagcatt ttatgaagtc gtccatggat
     9841 atagtactgc cgatataaaa ctgtggttca tggtcgatta tgaactttgg cgattcgtag
     9901 tctgtgtact ttacctttct tgtcagtttg gacacgttgt tgtcgctgtc acagacaaca
     9961 tatgttatgt tgcacacgcc cttttcaatg aattttgata tgctttcaat cagtattttg
    10021 ccggttagat ccccatcctg atcgtcgcgg gcagaaatcc cttcaaacaa cttgtcctta
    10081 tccattatgc tcacttcaat tacttttcga tcagttgtta tggtcgggta cgttccattt
    10141 gtttttactc catacacccc aaactggata aagtaaaaga tacctgtcac aatggacaag
    10201 agtaatatta ctgctctgaa aatcttcaca ataaccccca cctatcaata ttctcgtctt
    10261 ttaaaattct ccctggattt atataaaaca atgtttttgc atagtcagta ccgtagcggt
    10321 aacaaatata gtcgtacgct tcgtccagtc tggtggttct ttggtatggg ctgtgggcgt
    10381 cactggcaat aacgtgtaca agaccgtgtt tgagcatgta tttggccaat ttctgcgatg
    10441 ttgcgccaaa ttgaccaagt atgctccctt tgttgatttg gaagatacaa ccttggcttg
    10501 ccagttgcat tagcagactt gtgtcgtcat ggaatagctc atatctttcg ggatgtgcga
    10561 tgattggtat gagatttgca tttatgatgg atttgatatt tgtaagaatg ttatgagttg
    10621 agtcaaaaac aggtaattcg attaatatgt atctggtgtt gttgattgtt atcagtttgt
    10681 tgtccttcac taggtcaacc acatcttttg taaggtaaat ttccattcct ttgtataagg
    10741 taattggtat ttcttttgtt aattcctgca gtttcttaaa gcaattttcg aaccattctc
    10801 catagtagtt gctctgtatc gggatattgc tgtgcggcgt tgctaccaat gccctaactc
    10861 cgcgctgaca tgccatttcc agcattctga cggattcgaa gaaagacacg gacccatcat
    10921 cgacacctgg tatcaggtgt gcatgaatgt ctatcatttt atcgccttct taagtgaatc
    10981 caagatttcc cgtgttgttt gaaactgtgt atagagtgtt tttccttgct gtgttttggg
    11041 attgcacaca attatatcaa tgcattttgc taaagtaaat acttttaggt aatatctttt
    11101 atttcccaag gaaacaaaag tacatttttt cccaaaacag tttaattcct tttaacaagc
    11161 gcatccttgt ccagtactgc aagtattaca tcaatatcat tctcttgaag aaattcttta
    11221 attgcagtca aggccacttc aagcgcctca tctttcgggt atccataaat accgctcgat
    11281 attaaaggaa acgcaatgct cttacatccg tactccacag ccagttttaa tgaatttgtg
    11341 taagcattgt gtaacagacg cctgttttct tccctgctcc actggctgta tatgggtcca
    11401 gctgtgtgta ttacatactt tgctggcaga ttgaagcttg gagttatgac tgcctcacca
    11461 atttttattg gggcaagctt gtcgcaggct tcttgcagtt tgcttgctcc ggcagcccgg
    11521 aaaatcgctc cacaaacacc acctcccatt ttaagttcgg tgttggcagc gttgacaatt
    11581 gcatccacct ttagttttgt tatgtcctgc tgaacaattt caaatgacat tgcacaacct
    11641 ccatattgaa aaaaaaacca gcaaccctca tgaagttact ggtttgcacc attctagtca
    11701 tatcgctttg acaagctcat ctattaatac cagattgctt attctttcca gttttatttc
    11761 tgtcttcttg cagaactcag ccaaagtctc ataacaaatg tcacgcttgc agtatatcac
    11821 ttttggccgg ccatgaatgg ttattttctg cgttatgaaa tcatagattt cttttgctgg
    11881 attctgtgtt ggcaaaacaa ctttaacacc cagaattgat tcagacaagt tctccccaag
    11941 cactaccaat ctgcctatcg ctggcctgtc cagatcttcg tcataaatag ttgaatctat
    12001 gtacaccaaa tcaagctcca tttccgaatc ggtagctggc atctttttaa caagattgac
    12061 caggtcctca tcatgtattg gcattatttt attcacatcg ggggatactt caagagattg
    12121 ctgcgtgtaa gagtaattgt tgtttccaac aaagtttctg tagagcacgt taccgtcctt
    12181 gattgagaaa ttcatgcatc cttttgaata atctttgata aactcattga actgttcaaa
    12241 aaaacctttt aagaaaagca cttcgccttt gtccagcata tatggtgcat atccaggtct
    12301 gatcgataca aattgtatcc attcattttt gccgcgaaaa gatattccaa gttctctcaa
    12361 cttctcaagc tgcatgctaa gcatttcctg tctgtcacag aaatagcaga ttaaataatt
    12421 ctgttcacat ttgacctggt atgtatcgtc cttgttgctc agatattcag agtattttgc
    12481 cagagatgtt ctgttcggat aaattgcgat tgctattgca tcggagtcct caccagttac
    12541 tatacaataa catggttcat cacgcccggg tatatccagc gccagtatcc aatcatcgcc
    12601 gacatactgc caaggtttca ggctttttgt aactttagcc acttcatata gctctgtcca
    12661 ttcctgaacc gacgcatcca cgaacatttt aaaaattcct ttcaatttat gattatgaat
    12721 ataatataac acattttcac aggtttgtaa atagttaata ttacctcttg gagctggtgt
    12781 caaaatgtaa aaacatattt cacgctgcta acaaaatgga aataatcaag gatgcgtcca
    12841 tattcagttt tatgtagtgc ttgtcaatca aacatcattg aatgcaaaaa ttgttcaata
    12901 ctaggaagcc tttcttcctt ggccagaagt atgcccgttt tggaacaaaa gtcatccagc
    12961 agctcctcgc atatgtcttt agtgtagtgt attttcgcag gtctgcctgt ttttccaata
    13021 gcagaaaaaa ctgcctcaaa aatcaactca gcaagatcat cgtttggcag aactatcctt
    13081 gtttcaagga tatcacctga attcctgttg ctgattacca gaagccttac aatcggatcc
    13141 ctttcaaact gggtgtctct tattctagac tggacaaagg ttatgtcaat atcaaagctt
    13201 tctccactgt atggggtctt cttgattctt tgaatctgta tttcattttc atacttgtag
    13261 taatctgact gtgcattgac tatgggaacc tgcttctcaa tagtcatcca tctcttctca
    13321 tcatcgtcga aatatctgca taatgcaaag ccgtcatcaa accttacatc aatcttgttt
    13381 gtgttataat cttcaactgc attgcaaatc tgctctatgc attcagcaag cagcatgact
    13441 tctatcttgt ccgggtctgc aggagcatag catttccttt tggacaggaa aaacacccag
    13501 tccattcttc ctctgaacga aagccccagc tctttaatta tattatactg gcttttgggt
    13561 acactctccc ggctgccaat gcacatggta aatatgtcaa cggaacaaat gtcgttgaat
    13621 atttcctcat gggtgtaatc gggttttaga aaagtcttga taaattttgc ataggatgaa
    13681 gaatccctaa gtatgtgaat tgatataagt tcgggattct cgtctatgct tacataatac
    13741 atctcgtccc tatcgggcat ctttattgaa attgtgtcta aaaaacttaa tgattcccat
    13801 ggtcttaagt tcttcagcct tattgcatgt ccgtataatt gtgcccaatc atcaagtgtt
    13861 gctttaatca ttgatagtcc ctttcacatt gatttatagt atattatagc acaattatct
    13921 ttttctccaa aattgttatg gctgcttgcc aatatacgca agcagtcctc catcgacaaa
    13981 aaggacatgt ccatttacaa agtcagatgc tttcgacgca agaaagactg caggtccttt
    14041 caaatcttca ggtgttcccc atctgtttgc aggggttctt gccttgatgt aatcattaaa
    14101 aggattgcca tcttgtctta agggcgcggt ttgcggtgtt gcaatatatc ctggagcaat
    14161 accatttacc tgaatgttgt attgtcccca ttcagaggca aggcttcttg taagcatttt
    14221 gagccctccc tttgcagctg catacgcaca taccgtttcc cttcccagtt cactcatcag
    14281 tgaacatata tttatgattt ttcctcctcc gtttttaatc atatggggag ttacagcttt
    14341 tgatactata aagggagcaa ccagatccac gtctatgact tccttgtact cgtcagcagt
    14401 catgtcaacg gcaggtatcc tttttatgat acctgcattg ttcacaagaa tatcaatgtg
    14461 tccatactct ttaatgagca tgtctgcaaa ttcctttact tcgtcctcct tggtgacgtc
    14521 acatatatac ccctttacat taagaccgtg cttctcgtac tcctttattc cctttttgac
    14581 attctcttca tgtatgccgt tgaatacaac agttgctcct gcctcggaca gagctgaagc
    14641 aatagcaaaa cctattccat aggaacctcc agttacccat gcaacttttc catcaagtct
    14701 gaacattttg tgcccccctt atttcaattc caaagtagga atatggtcca tgtcggtaaa
    14761 ttccctgttt tcaccagcca ttgcccatat gaaagaatat gatcccgtgc cgcatcctgc
    14821 atgtattgac caggatgg
//
LOCUS       DAONVY010000003        14028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_3323_length_14028_cov_4.289702, whole genome shotgun sequence.
ACCESSION   DAONVY010000003 DAONVY010000000
VERSION     DAONVY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3323_length_14028_cov_4.289702"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..2192)
                     /locus_tag="PLI11_00170"
     CDS             complement(108..2192)
                     /locus_tag="PLI11_00170"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPZ51314.1"
                     /translation="MHRTFTMELAGRPLIVETGKMAQLANGTCLVRYGETVVLSTVTA
                     SKEPREGIDFFPLSVDYEEKLYSVGKIPGGFIKREGKPSDKAILVARAIDRPIRPLFP
                     KDLRNDVVVVNTVLSVDQDNSPEITAMIGTSVALSISDIPFNGPVGAVSIGLVNDEIV
                     INPKTKERDASDMYVTLAGTKDKICMIEAGANEVPEDVMLKALIKGHGTIKKICAFIE
                     DIVNEVGKPKFEYKSAQIPEEIMEAVKEFCYDEIKVAVLTDDKTVRDARMDDITKRVQ
                     EALAERFPDSASVISEAVTSCEKKIVRDYLLKEHKRVDGRALDEIRQLEAEVAVLPRT
                     HGSGLFRRGLTQVLTVATLGPMSDIQMLDGIDLEEEKRYMHHYNFPSYSVGEARPTRG
                     PGRREIGHGALAERALEPVIPSEEEFPYAIRLVSEVLMSNGSTSQGSVCGSTLALMDA
                     GVPIKRPVAGISAGLVVNEEDENDFVTFIDIQGIEDFFGDMDFKVAGTEKGITAIQVD
                     IKVQGLSYEIIRQALEITRRGRMQIINDVLLKAIPEPRKEVSKYAPKMFTMKIDPDKI
                     REVIGTGGKVINKIIEETDVKIDIQDDGTIYVATPEKENAEKAMKMISGIVGEVEIGA
                     EYEGKVTRIMNFGAFVEFVPGKEGLVHISKMSKKRIKKVEDVLNVGDRVEVKVIDIDK
                     QGRINLALKDIE"
     gene            complement(2383..2646)
                     /gene="rpsO"
                     /locus_tag="PLI11_00175"
     CDS             complement(2383..2646)
                     /gene="rpsO"
                     /locus_tag="PLI11_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPZ51315.1"
                     /translation="MTKERKQEIIKEFAIHEGDTGSPEVQIALLTERINHLNEHLKKH
                     KKDHHSRRGLLMMVGQRRGLLNYLMKVDVNRYRSLIERLELRK"
     gene            2987..3346
                     /locus_tag="PLI11_00180"
     CDS             2987..3346
                     /locus_tag="PLI11_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPZ51316.1"
                     /translation="MRSLIAECRDPEEMLSLLRKRRIERKRRTIRLRVVAFVFIVTSL
                     LLVVTVCAKSIKTKPQTTYKTVVVEQGDTLWDLAKEYYPDMEIRRAVYELKKINNLQD
                     ATIYEGQSLKVPDIDND"
     gene            3394..3894
                     /locus_tag="PLI11_00185"
     CDS             3394..3894
                     /locus_tag="PLI11_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51317.1"
                     /translation="MYFKTEEIRIDNLPYDIEEFKKTAVEKMVDPVNTAVLFIFALNI
                     YAEDADKGKEFLSFLIHDFENAISFSNIAKNNNIAKSYLKGAEPSNKYTPSQPLTVVV
                     KYDEERGRIKHLKTVYIGCGGVDSYRPLTLVRVKRRKLPFKHKHDLWFVYDYPSIILD
                     VKEADQ"
     gene            3891..5057
                     /locus_tag="PLI11_00190"
     CDS             3891..5057
                     /locus_tag="PLI11_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPZ51318.1"
                     /translation="MRFIHLADLHIGKRIKEFSLLEDQAYILERIVEITEKEKADAVI
                     IAGDLYDKSVPPGEAVPVLDDFLTRLSEMDKTVFIISGNHDSSERLSFGSRLLKKNNI
                     HIISTFDGSLSKTVMEDEYGSINVFALPFVRPARVQKFFPDKTISSYEDALVDIINDA
                     KGKGILVPNERNILICHQFVTGTSSPILSDSESTIVGTLENISCEIFEDFDYVALGHL
                     HCPQSVGRETIRYAGSPLKYSFSEVNHKKSVTVVDIDKNKVSIKESPLTPGRDMRKIK
                     GKLKNLISPEVCEEICSDFIHATLTDENDLYEPLATLRAYYPYILHMEHIKESEFSNE
                     NNETSVYDRISKNPIKLFKEFYEETKQKEFHEHKQKLLEKVLKEELEEFCHDTH"
     gene            5044..8160
                     /locus_tag="PLI11_00195"
     CDS             5044..8160
                     /locus_tag="PLI11_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPZ51319.1"
                     /translation="MIPIRLKICGFGPYKDETVLELDKLGRSGLFLITGDTGSGKTTI
                     FDAIAFALYGVASGKNRNDTSGLRSDFATSNTQTYVEFTFLYKGKEYVVRRNPRYTRA
                     KKVGSGTTEENAYATLELDNKVIVDGDRNVTNYIRELFALDWNHFRQVAIIPQGEFMD
                     FLLASSGEKEELFRKIFDTSLYSRIQDILKKKSQDYLKITEEYKNSLVQYVNGIKIPK
                     ESNLYNEFSEYKDNNAVVLINEIMDLVARQNKEDLYKVNENRKQFKEIDDTLTLKKVE
                     YSEAEKLIKYFNDLKEYEKEYNILLEKDEYINRQIDNISRIEKSVNLILPLDKKMQED
                     YADFIKIEFAVKKHRENINDLVVKLEKAEKDLTKEQENEPYRRHLNARIEGIKSKLPE
                     YEKLGQLKDKMSSLQKQTDDAQKKLNKVKGEADKLSQKEKDLSYHLATLKDAESNYYN
                     FKYALDNARKQEEEIKQHIATIKEITDVQTKYSQLEKQYKKHEKEYLHADNIYAVKLS
                     EFLRNQAGILARNLEENTPCPVCGSLEHPNPASYQDTATTKEELELLKNDKETKEKRL
                     NKISIELSQWKERLRLLVDIAANMDKDFAIDSIPYALDKLENKLKALNIGLLEERFFK
                     EEKRLEEYKKMQVELEKTKEDLSNKKQEHDAFFDMFNNLNSDRIKAEAEYNSLRTSFE
                     YKDKTEAENAIRTLSEEYDKSMEKLDLCIQKVNLLKTELEKERVLLSKNESDLASTRE
                     RFENLKKEFHDSLVVHNISEEMYKELVECKDQLESMKEEVETHKEKKLNAKNKINMLK
                     QTLEGKTMPDLQGIQESIKELEEKKEKIQNEYEILFSRYSANKDSLENIRKTNVHLLQ
                     ASQSYVEVEELSSVANGNCKGVRKITFERYVQAMYFEEVIKSANKRLYAMTERYQLAR
                     KEETDMRQGVGLDLEVIDAYTGKSRSVKSLSGGETFIASLALALGLSDMIQKNSGGIH
                     IDTLFIDEGFGTLDSECLELALKTLGALSQNNRLISIISHVPELRERIDRKIVVTKTR
                     LGSNAHIV"
     gene            complement(8216..9577)
                     /locus_tag="PLI11_00200"
     CDS             complement(8216..9577)
                     /locus_tag="PLI11_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPZ51320.1"
                     /translation="MARYLKTFTAVFLVLLFILLSGCSRGDVQSGALPPGYKANVSGH
                     ISVTYFLALDDESKLSLSYFAQAFQDKYKDATVNLDFSTGDNRDARIASGDIGDVFFC
                     WEDEIYRYAVENKVLMPLSAYLEVLDIDISNVYSGIYDLGVAEGQLYMAARDHTHIVL
                     FYNATALQEVGLDTPPADWTWEDFKDYCRKLVREEDDGTISRVGAGWDVSYGPHLIPF
                     LEGWGGKWYDQVNKRVNFVSDQNVLQGFTEQIQAMEEFLLKPEGTSASVAIASRYKVM
                     NEDVIFKAGVFPGLHNTGLAYEMKGLDWNIANWPRFPIHRVGTGATGFAVYNRTKRPD
                     TAAAFALFLFTDEGQRAYNGQTGGSVPLTKNLAQEDFWRQPYDPDEINYDAFVSFPEA
                     DTVGKFHCRVPNAVANIITQRMLNVFVRHFNGSQSYYDSLKEIEELANEKWTELFENE
                     TRP"
     gene            9744..10922
                     /locus_tag="PLI11_00205"
     CDS             9744..10922
                     /locus_tag="PLI11_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HPZ51321.1"
                     /translation="MSEKRSKSRKRIKLRAPSTKALLYIFLFFLVCSAISVALFKISE
                     KNPDIVDEYYNSFVFKAITLPSKIFVSIFPFSVSEIALITVIVFVISYFIRTIVLTVK
                     RIRKKQGKIYMPAVRYILSIGILITGIITMFVVNGGLNYNGITFADRSGLVLVETSTE
                     ELEELCMFLGEQAAKARKLLPENDKGVISPDVSVFELAKKAKDGYKTIEDTYPYLKGF
                     YPKAKPVIFSHFMCYTKITGIYPYIIPEPNINYKTPIMSLPSTINHEMAHQRGISRED
                     EANFIAYLASINNPDPLFQYSGYYLALNYSLNALSAHNGDASRRVYEALDKGILRDMQ
                     AAREFWKQFETPKNIVATVSENVNNKYLETVNVEDGVHSYGRMVDLLLAYRRAEKEAN
                     "
     gene            complement(11048..12211)
                     /locus_tag="PLI11_00210"
     CDS             complement(11048..12211)
                     /locus_tag="PLI11_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPZ51322.1"
                     /translation="MNKKLKEILQSKSFYIFSSIIIGILLWLFVLNYSNPVIERTLEI
                     PLTIENQNYPATKELINTTVTYPTVVTVTVKGREDIVNNLLLSELYSSVDFSEVKEPG
                     EIKLNVSKPTCSRYGIRIEDYYPKQIDFVFDKRMETYLDVRLIYDDSLLKENYKFINV
                     TAEPDSIPVSDLSSLVEKLEEIQVNLSDSIARNSIDGNKTASFIGRYISKRGEDVSHY
                     FNNTVTITVKIEVAKEVPLVYSVVGEPETDFYIDSSSLSSTTVLLQGSAADLSRISSI
                     NLGAYDVTGRSEDYVREINISDFLPERVSVYGNNKVTLTVDIEEYQIKEFEITDTILS
                     KPGMELDVYEYVISPDVFIIKIKGKEKDLKTLQRSSLNPTLDLTDRSVGIYTG"
     gene            complement(12208..13092)
                     /gene="cdaA"
                     /locus_tag="PLI11_00215"
     CDS             complement(12208..13092)
                     /gene="cdaA"
                     /locus_tag="PLI11_00215"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPZ51323.1"
                     /translation="MKIIAANPEIGEIFTYLTEYLGIKDTWSFFRIVIDIGIVSFVVY
                     KLAQLLKDTRALQLIKGILLVLVISLLADFIGLTTVSYLTSLALQILPFIVIITFAPE
                     IRRALEGIGKNKLKDFFRSISYQESQETARMIDEVVNAVEGLVRDKVGALIVFEKDIN
                     LGEIVRTGVIIDSVVSSQLLQLIFIPNTPLHDGAVIIRNNKIHAAACVLPLTGDLHIS
                     KELGTRHRAGIGVTETSDSVSIIVSEETSKVSIARRGTLIRNITPNNLRTILTNEFVR
                     KDDKRKKDIFTFWKGRSK"
     gene            complement(13304..13846)
                     /locus_tag="PLI11_00220"
     CDS             complement(13304..13846)
                     /locus_tag="PLI11_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51324.1"
                     /translation="MAKGNKVFDTEFSGFNKKQVNEYIEKLVSQYQQSLSEKAKECDE
                     LRAKNEQLASKLNELSTAYIQAQEEKTKIADVLINAENTAKNIIAKAQEESAKERERL
                     SIQADEKRMLIVDLNKIIRDMKLEVEEMIENAKSSLDNAVNQIKERMDAEKEQIIRRI
                     EEINAKYAEKEEVEEEAKED"
BASE COUNT         4346 a         2969 c         2617 g         4096 t
ORIGIN      
        1 tttacatttt ttcttctttc ttccttatgc cgatatagag caatttccta taacaaaaaa
       61 aaggtgtgca attgcttgca cacctttttg taccagtcat accaatctta ttcaatatct
      121 ttcaatgcaa ggtttattct tccctgcttg tctatgtcaa ttaccttgac ttccaccctg
      181 tcgccaacat tcagaacgtc ttccactttc ttaatgcgtt tcttcgacat cttggagatg
      241 tggacaagac cttccttgcc tggtacaaat tcaacaaagg ctccaaaatt cataatcctt
      301 gttacctttc cttcgtactc agcaccaatt tcaacttctc caacaattcc agatatcatc
      361 ttcattgcct tttccgcatt ttctttttcc ggtgtggcaa catagatggt tccgtcatcc
      421 tgaatgtcta tcttgacatc ggtttcctca attatcttgt tgataacctt tccacctgta
      481 ccaatgactt ccctaatctt gtcaggatct atcttcattg tgaacatctt tggtgcatac
      541 tttgaaactt ccttccttgg ttcaggaata gctttaagca gtacatcgtt gattatctgc
      601 attctgcctc ttcttgttat ttccaaggct tgtctgatta tctcatagct gagaccttga
      661 accttgatgt ccacctggat tgcggttata cccttctcag ttccagcaac cttgaagtcc
      721 atatcgccaa aaaagtcctc aataccctgg atatctataa atgtaacgaa atcattttcg
      781 tcttcctcat ttacaaccaa acctgcagat atgcctgcca ccggtctctt aataggtacg
      841 cctgcgtcca taagtgcaag agttgaaccg caaacgctgc cttgggatgt ggaaccattg
      901 gacatcagca cctcggaaac cagcctaata gcataaggga actcctcctc tgacggaata
      961 acaggctcca atgcacgttc agccaaagcg ccatgaccaa tttcccttct tccaggacct
     1021 cttgtaggtc ttgcctctcc cacgctgtag gatgggaagt tgtaatggtg catatatctc
     1081 ttttcctctt caagatcaat accatccagc atttggatgt cggacatggg gcccaaggtt
     1141 gccacggtca aaacctgcgt aagtcctcgt ctgaacagac cggagccatg ggttcttgga
     1201 agaacggcta cttctgcttc cagctgcctg atctcgtcaa gcgccctgcc gtcaactctt
     1261 ttatgctctt tcaacaggta atcgcggact atcttctttt cacaggaagt aactgcctca
     1321 ctgattaccg atgcgctgtc aggaaatctt tctgccaagg cttcctgtac cctctttgtt
     1381 atgtcatcca ttcttgcatc tcttacagtc ttgtcgtcag taagaacagc aacctttatt
     1441 tcatcataac aaaactcttt tactgcttcc atgatttcct caggaatctg ggcagacttg
     1501 tactcaaact ttggttttcc aacttcgtta acaatatctt caataaaagc gcatatcttt
     1561 tttatggtgc catggccttt aatcaacgcc ttaagcatta catcctctgg tacctcgttt
     1621 gcgcctgctt caatcataca tattttatcc tttgtgcctg caagagtgac atacatgtca
     1681 ctggcatcac gttctttggt tttgggattt ataactatct catcattgac aagaccgatc
     1741 gacactgctc ctactggtcc gttgaaaggt atgtccgaaa tggacagtgc gaccgatgtg
     1801 ccaatcatgg cggtaatttc aggtgaattg tcctgatcaa ctgataaaac agtatttacc
     1861 acaaccacgt catttctcaa atctttgggg aataaaggtc tgataggtct gtctatcgct
     1921 cttgccacca atattgcctt gtccgaaggt ttaccctctc tcttgataaa tcctccggga
     1981 atttttccta cggagtacag tttttcttca tagtccacgc taagtggaaa gaagtcgata
     2041 ccttcccttg gttccttcga cgctgttaca gttgacaata caactgtttc accatacctg
     2101 acaagacatg ttccgtttgc caattgtgcc atcttccctg tttcaactat taatgggcgt
     2161 ccagctaatt ccattgtaaa tgttctgtgc ataaattctc ctccttttag caacaggacg
     2221 cgtgcggtag ctcgtagatt ctatgcctgc aagtacagac acacaatcta ctctctactg
     2281 cctgcgcccc ttgctatctt aaatttttat tattttaacc aattcacagc gaaaaataaa
     2341 cagaaacaaa gagcggaaaa aaccgctctt gaaaacgaaa gactactttc taagttcaag
     2401 tctttcaatt aatgaacgat atctgtttac atccaccttc atcagatagt taagcagacc
     2461 tcttctctga ccaaccatca taagcagacc tcttcttgaa tgatggtctt tcttatgctt
     2521 tttgagatgt tcattaagat ggttgattct ctctgtaagc agtgcaatct gaacttctgg
     2581 agaacctgtg tctccttcat gaattgcaaa ttccttaatg atttcctgtt ttctttcctt
     2641 tgtcataatt ttctccttta tatataaata ttttgcccgg aatcctagta tatggttgga
     2701 gcttccataa accaggaaac ggtctaaaca attataccac taattatttt ttatgtaaag
     2761 cacgacattc caaccgatcc tctttcccaa ttctcatatg atcttccaga actgctaaat
     2821 gcaaaatggt tttagtaata ttataccctt cactattcat acaaaccaat caaacaaatt
     2881 tttgattttt ttgaattttt ctcgaaattt tctattgaca cgaccttttt actatgctaa
     2941 catatgttcg tacgaacata tgtgcgattt tggggaggtt ggtacaatga gaagtttgat
     3001 agcagagtgc agagatccgg aagaaatgct aagtctattg agaaaaagaa gaattgaaag
     3061 gaaaagaaga actataaggc taagggttgt agcgtttgta ttcattgtca cgtctctatt
     3121 gcttgtagtt acggtttgtg caaagagcat aaaaaccaaa ccccagacaa cgtataagac
     3181 agtagtggtg gagcagggag acactctttg ggacttggca aaagaatatt atcctgacat
     3241 ggagataaga agagcggtat atgaactaaa gaagataaac aacttgcaag acgcaacaat
     3301 atatgaagga caaagcctta aagtgcccga cattgacaat gattgagata aatattatta
     3361 ttgttggtga taaatatttt agggtgttgg gacatgtact ttaaaacaga ggaaatcaga
     3421 atagataatc tgccttatga tattgaagaa ttcaagaaaa cagctgtaga aaaaatggtt
     3481 gatcccgtta atacagctgt tctatttatt tttgccctga atatctacgc agaagacgca
     3541 gacaagggaa aagaatttct ttccttcctt atacacgact ttgagaatgc gataagtttt
     3601 agcaatattg caaaaaacaa caacatagca aaatcatatc tgaaaggtgc cgaaccttcc
     3661 aataaatata ctcccagcca gcctcttact gttgttgtta aatacgatga ggaaagaggc
     3721 agaataaaac atctaaaaac cgtatacatt ggctgtggcg gtgttgatag ttacagacct
     3781 cttacactag tcagagtaaa aagaagaaag ctccccttca aacacaagca cgacctttgg
     3841 ttcgtatatg attacccctc cataatattg gatgtaaagg aggcggatca atgagattta
     3901 tccaccttgc cgacttgcac atcggtaaaa gaataaagga attcagcctg ctcgaagacc
     3961 aggcatacat acttgaaagg atagttgaga ttactgaaaa ggaaaaggcg gatgctgtca
     4021 tcatagcagg cgatttatac gacaaaagcg tccctccagg agaagcagta cctgtgctgg
     4081 atgattttct tacccgtctt tccgaaatgg acaagactgt tttcataatc tccggaaatc
     4141 acgactcaag cgaaagactg tcttttggaa gcagacttct taagaaaaac aatatacata
     4201 taattagtac gtttgatgga agtttgtcaa aaaccgtaat ggaggacgaa tacggcagta
     4261 taaatgtttt tgccctgccc tttgtccgtc ctgcaagagt tcagaaattt tttccggaca
     4321 agacaatatc aagctacgag gatgcactgg ttgatataat aaatgatgca aagggcaaag
     4381 gaattcttgt gccaaatgaa aggaatatcc tcatttgcca ccagtttgtt acaggtactt
     4441 caagccccat actctctgat tcggaatcaa cgatagttgg cacgcttgaa aacataagct
     4501 gtgagatatt tgaggatttt gactatgttg ccctagggca tcttcattgt ccgcagtccg
     4561 ttggcaggga aaccatacgc tacgcaggtt cgcctcttaa atactccttt tcggaagtga
     4621 accataaaaa aagcgtgacg gttgttgaca tcgataaaaa caaggtgagt ataaaagaga
     4681 gtccgctgac tcctggaagg gatatgagaa agataaaggg aaagcttaaa aacctgatat
     4741 ctcccgaggt ttgcgaggaa atttgcagcg acttcataca tgccaccttg acggatgaaa
     4801 atgatcttta cgaacccctt gccactttac gtgcatatta tccctacata ctccatatgg
     4861 aacatataaa agaatcggag ttttcaaatg aaaacaatga aacatctgta tatgacagga
     4921 taagcaaaaa cccaataaag cttttcaagg aattttatga ggaaaccaag cagaaagagt
     4981 ttcatgagca taaacaaaaa ctgcttgaaa aagtgttgaa agaagaatta gaggagtttt
     5041 gccatgatac ccattagact taaaatatgt ggttttggtc catacaagga cgaaacagtt
     5101 ttggagcttg acaaacttgg ccgcagcggc ctttttctca taaccggtga tacaggcagc
     5161 ggcaaaacaa caatttttga cgccattgcc tttgctctat atggtgttgc cagcggaaaa
     5221 aacagaaacg acacttcagg gttaaggagt gattttgcca ccagcaacac ccaaacctat
     5281 gtggagttca cattcctgta caaaggaaaa gagtatgtgg tgcgaagaaa tccaagatac
     5341 acaagagcaa agaaagtagg aagcggaacc accgaagaaa acgcatatgc caccctggaa
     5401 ctggataaca aagtcatcgt cgatggggac agaaatgtaa caaattatat cagggagctt
     5461 tttgctcttg attggaacca tttcagacaa gtagcaataa ttccccaagg cgagttcatg
     5521 gatttcctgc tggcatccag tggtgagaaa gaagagcttt tcagaaaaat tttcgacaca
     5581 tccctgtaca gcagaataca ggatatcctg aaaaagaaaa gccaggatta tcttaagata
     5641 acggaggagt ataaaaacag tcttgtccag tatgttaacg gaataaagat tcccaaagaa
     5701 agcaaccttt acaatgaatt ctccgaatac aaggacaaca atgcggtggt tttgattaat
     5761 gaaatcatgg atttggttgc aaggcaaaac aaggaggatt tatataaagt taatgaaaat
     5821 cgaaagcaat ttaaagaaat tgatgacacc ttgactttga aaaaggttga atacagtgaa
     5881 gcggaaaaac tcataaaata ctttaatgat ttgaaagaat atgaaaagga atacaatatc
     5941 cttcttgaaa aagacgagta cataaacagg caaattgata atatttcacg aattgaaaag
     6001 tcagtcaatc tgattcttcc tcttgataaa aaaatgcagg aagattatgc ggactttatt
     6061 aaaattgagt ttgccgtaaa aaagcacagg gaaaatataa atgaccttgt agtaaagctg
     6121 gaaaaggcag agaaagacct gaccaaggag caggagaacg aaccctatcg cagacatcta
     6181 aacgccagaa tagaaggcat taaatccaaa ctgcccgagt acgagaaatt aggccaatta
     6241 aaggacaaaa tgtcctcact gcaaaaacag acggacgatg cacaaaagaa attgaacaag
     6301 gtaaaaggag aggcagacaa gctttctcag aaggaaaagg atttgtcata tcatcttgct
     6361 accttaaagg atgcagaatc caactactac aactttaaat atgctttgga taatgcaagg
     6421 aaacaagaag aagaaatcaa acaacacatt gcgaccataa aagaaataac tgatgttcaa
     6481 acgaaataca gtcagcttga aaagcagtac aagaaacatg aaaaagaata cttgcatgca
     6541 gacaatatat atgccgtaaa actctctgaa ttcctaagaa accaggcagg catactggca
     6601 agaaatcttg aagaaaacac tccctgtccc gtatgcggct ctttggagca cccaaatcct
     6661 gcttcctatc aggatactgc caccaccaag gaagagttgg aacttttgaa aaacgacaaa
     6721 gagaccaagg aaaaaagact gaataaaata tccatagagc tttctcagtg gaaagaaaga
     6781 ctaaggcttc ttgtggatat tgcagcaaat atggacaagg actttgctat tgatagtatt
     6841 ccatatgcct tggacaagct tgagaacaag cttaaagctt tgaatattgg tttgctcgaa
     6901 gaaaggtttt tcaaagaaga aaaaaggctt gaagaataca aaaaaatgca ggtggagctt
     6961 gaaaaaacaa aggaggattt atcaaacaag aaacaggagc atgatgcatt tttcgatatg
     7021 ttcaacaatt tgaacagtga caggatcaaa gcagaggcgg aatacaactc tttaagaacc
     7081 tcctttgagt acaaagacaa aacagaagcg gaaaatgcca taagaaccct ttcggaagag
     7141 tatgacaaat caatggaaaa acttgatttg tgtattcaaa aggtcaactt actcaaaaca
     7201 gaactggaaa aagaaagggt gctcctttca aaaaatgaat cggatctggc ttcaaccagg
     7261 gaaaggtttg agaatttgaa aaaggaattc cacgattcac ttgttgtcca caatattagt
     7321 gaggaaatgt ataaggagtt ggttgaatgc aaggatcagc ttgaatccat gaaagaagag
     7381 gttgaaaccc acaaggagaa aaagttgaat gcaaagaaca agataaatat gttaaaacaa
     7441 acccttgaag gaaaaaccat gcctgatttg caaggcatac aagaaagcat taaagagctg
     7501 gaagaaaaga aagaaaaaat ccaaaacgaa tacgaaattc ttttttccag gtattctgca
     7561 aacaaagaca gtttggagaa tattagaaag acaaatgtac accttttgca agcaagtcag
     7621 agctatgtgg aagttgaaga actgtcttcc gttgccaacg gaaactgcaa aggagtaagg
     7681 aaaattactt ttgagaggta tgtccaggca atgtactttg aagaagttat caaaagtgca
     7741 aacaagaggc tgtatgctat gactgaaaga taccagctgg caagaaaaga agagactgac
     7801 atgcgtcaag gtgtaggttt ggatcttgaa gttatagacg cttacacagg gaaaagcaga
     7861 agcgtaaagt ccctttccgg tggtgaaacc tttatcgcct cactggctct tgcacttgga
     7921 ctttccgaca tgattcaaaa aaactcagga ggcatccaca ttgacacctt gtttatagat
     7981 gaaggttttg gtactttgga ctctgaatgc ctcgaacttg cactaaagac cttgggtgcc
     8041 ttgtctcaaa acaaccgctt gataagtatt atttcccacg ttcccgagct tagagaacga
     8101 attgacagaa aaatagttgt cacaaaaaca agacttggaa gtaatgcaca tattgtataa
     8161 ataataaaca ccggggttga acccggtgtt tatctattca ttaactattt ttacattatg
     8221 gtctcgtttc attttcaaag agttcagtcc atttttcatt tgccagctcc tctatttctt
     8281 tcaagctgtc atagtagctt tgcgaaccgt tgaaatgcct tacaaacaca ttcagcattc
     8341 tttgagttat aatatttgca acggcattag gaacacgaca gtggaacttt cctaccgtat
     8401 cggcttcagg gaagctgaca aaagcatcat agttaatctc gtctggatca taaggctgtc
     8461 tccagaaatc ctcttgggcc aggttctttg ttagaggtac cgaaccacca gtttgtccat
     8521 tgtatgccct ctgtccttcg tctgtaaaca ggaacagcgc aaatgccgca gcggtatcag
     8581 gtctctttgt tctgttgtat acagcaaatc ctgtagcacc tgttccaact ctgtggattg
     8641 ggaatcttgg ccagttggca atgttccaat ccaatccctt catctcataa gccaaacctg
     8701 tattgtgaag tccggggaac acgcctgctt taaatataac atcttcattc ataaccttgt
     8761 atctggatgc aatcgcaaca cttgccgaag taccttctgg cttcaacaag aactcttcca
     8821 tagcctggat ttgctcggta aatccttgaa gtacattttg gtcacttaca aagtttactc
     8881 tcttgtttac ctgatcgtac cattttcctc cccagccttc aaggaatgga attaaatgcg
     8941 gaccatatga aacgtcccat ccagcaccaa ctcttgaaat cgttccatcg tcttcctcac
     9001 gaaccagctt tctgcagtaa tctttgaaat cttcccatgt ccagtctgca gggggagtat
     9061 ccaagcctac ttcttgtagt gcagttgcat tgtagaacag aactatatgt gtatggtctc
     9121 ttgcagccat gtaaagctgg ccttcagcaa cacccaggtc gtaaataccg gagtatacgt
     9181 tgctgatatc gatgtcaaga acttcaagat aagcactaag cggcatcaaa actttgtttt
     9241 ccactgcgta cctgtaaatc tcatcttccc aacagaagaa gacgtctccg atgtctccgg
     9301 atgctatacg cgcatctctg ttgtcaccgg tcgaaaagtc caggttgaca gtggcatcct
     9361 tgtatttatc ctgaaatgct tgagcgaaat acgacagaga caacttgctt tcatcatcta
     9421 acgcaaggaa atatgtaacg cttatgtgcc ctgatacgtt cgccttatat cctgggggca
     9481 aggcgcctga ttgaacgtcg cctctactac atccactcaa taaaataaag agaagtacca
     9541 gaaaaacggc tgtaaaagtt ttcaaatacc tggccatcat aaacctcctg attcttaata
     9601 acttaattaa atgataacac atagtaaaga aaatttctac atatttttaa tattattttg
     9661 catatttttt ttggaacaat ttatattttg tcagcaagcc caattatgtt ataattatat
     9721 tacatttttg tggaggtact gacatgagtg agaagagatc aaaatcaaga aaaagaatta
     9781 aactacgtgc tcccagtact aaggcactct tgtacatttt cctgtttttt ctggtttgca
     9841 gtgctatatc tgtagcattg ttcaagatat ccgaaaagaa ccctgatata gtggacgaat
     9901 attacaattc attcgttttc aaagcaatca cactgccttc aaaaatattt gtctcgattt
     9961 tccccttctc agtatccgag attgctctga taactgtaat tgtatttgtc atttcatact
    10021 ttatccgcac aatagtgctt acagtaaaaa gaatacgtaa aaaacaaggc aaaatataca
    10081 tgcctgcagt ccgctacata ttatcaatag gaatattgat tacaggaatc ataaccatgt
    10141 ttgttgtaaa cggaggtctc aattacaacg gaataacctt tgccgacagg tccgggcttg
    10201 tgcttgttga aacatctaca gaggagctgg aggaactctg catgtttctc ggagaacaag
    10261 cagccaaggc aagaaaactc ctcccagaga atgacaaagg ggttatctcc cctgacgtat
    10321 cagtatttga acttgcaaaa aaggcaaaag acggctacaa gaccatagaa gatacatacc
    10381 catatcttaa aggattttat ccaaaagcaa aacctgtaat attctcacat tttatgtgct
    10441 acacgaagat aacaggaata tatccgtaca taataccgga accaaatatt aactacaaaa
    10501 ctccgataat gagtctgcct tcaaccataa atcacgagat ggcacatcaa agaggcatat
    10561 cccgtgaaga tgaggcaaac tttatagcgt accttgccag tataaacaat ccggatccct
    10621 tgttccagta ttcaggatac taccttgctt taaactactc acttaacgct ttgtccgcac
    10681 ataatggaga cgcatcaaga agagtatacg aagcactcga caaaggaata ctaagagaca
    10741 tgcaggctgc aagggaattc tggaagcagt ttgaaacccc caaaaacata gtagccacgg
    10801 tatctgaaaa tgttaacaac aaatatctag aaacagtaaa tgtagaagat ggagtacaca
    10861 gctacggcag aatggtagat ctgctgcttg cgtacagaag agcagaaaag gaagccaact
    10921 agcagcttcc tttttttata tccaattcat atccaaaatt tatggttctt ccggttcttc
    10981 cgtctcatcc ggttccagca aggagatgtt tacctcgtag atatattctc ctattagttc
    11041 aaatctttca tccagtgtaa attccaacac ttctatcagt caaatcaagg gtaggattca
    11101 gcgagcttct ctgcagagtt ttcaaatcct tctccttgcc ctttatttta atgataaaca
    11161 catcaggtga tattacatac tcataaacat caagctccat tccaggcttg ctgagtattg
    11221 tatcagttat ttcaaactcc ttgatctgat actcttcaat atccacggtc aatgtaactt
    11281 tgttgtttcc gtaaactgaa accctctcag gcaggaagtc agaaatattt atttctctga
    11341 catagtcttc acttctgcct gttacatcat aagccccaag attaatactg cttattctgc
    11401 tcaaatctgc agcactgccc tggagcagaa ctgtagtaga ggaaagactt gacgagtcga
    11461 tatagaaatc agtttcaggc tctccaacaa cactgtatac caaaggaact tcttttgcca
    11521 cttctatttt cactgttatg gttaccgtgt tgttgaaata gtgggaaaca tcctctcccc
    11581 tttttgaaat atatctgcca ataaagcttg cagtcttgtt gccgtctatc gagtttcttg
    11641 caatactgtc agacagattt acctgaatct cttccagttt ctccaccaat gaagaaaggt
    11701 cgctaacagg tatgctgtca ggctccgcag ttacattgat gaatttgtag ttttctttta
    11761 acaaggaatc gtcatatata agtcttacat ccaggtaagt ctccattcgc ttgtcaaaca
    11821 caaaatctat ctgctttgga tagtagtctt caattctaat gccgtatctt gagcaggtgg
    11881 gtttgcttac gttaagcttt atctctcccg gttccttgac ctctgaaaaa tccaccgaag
    11941 aatacagttc actaagaaga agattgttta caatatcttc cctgcccttt acagtaactg
    12001 ttacaaccgt tggataagta acagtcgtgt taatcaactc ttttgtagca ggataattct
    12061 gattttctat ggtaagaggt atctcaagag ttctttcaat cacaggattg gaatagttga
    12121 ggacaaacag ccacaaaagt attcctatta tgatggagct gaatatgtaa aagcttttgc
    12181 tctgaagtat ctccttaagc tttttattca tttgcttcgc cccttccaaa atgtaaaaat
    12241 gtccttcttc cgtttgtcat ctttcctgac aaattcatta gtcaatatgg ttcttaagtt
    12301 gttaggggtt atgtttctaa tcaaggttcc tcttcttgct attgaaacct tgctggtttc
    12361 ctcggataca attatactga cactatcgga tgtttcagta acgccaatac cagctctgtg
    12421 tcgtgtcccc agttccttgc ttatatgaag atctccggta agaggcagca cgcacgcagc
    12481 tgcatgtatc ttgttgttgc gtataataac ggcgccatcg tgaagtggtg tgtttggtat
    12541 gaatatgagc tgcaggagtt gcgagcttac aacactatct atgattactc cggttcttac
    12601 aatttcccca agatttatat ccttctcaaa aacaatcaat gcgccaacct tgtctcttac
    12661 gagaccttct accgcattta caacttcatc tatcattctt gcggtttcct gggattcctg
    12721 atagctgatg gatcggaaaa agtccttcaa cttgttcttt cctatgccct caagagccct
    12781 tctgatctcc ggcgcaaagg tgataataac gataaacggc aaaatctgca aggcaagact
    12841 cgtcaaatag gacacggtag tcaaccctat aaaatcggcc agcagcgaaa ttacaagcac
    12901 cagaagaatg cctttgataa gctgaagggc tctggtgtct tttagcaact gagccagttt
    12961 atagaccaca aaggatacaa taccaatgtc tattacaatc ctgaaaaaac tccaggtatc
    13021 ttttattccc aagtattcgg taagataagt gaaaatctcg cctatctccg gattagccgc
    13081 aataatcttc aaaaaatcac cttatctata taataaataa aacaatgtta atgctatagt
    13141 ggatgatgcc attgcaattg ccaggatcag acatactatt cttactatct tcttctttag
    13201 ttcctgtttc ctcacataag aattacgcat aatcttgtct cccataaata acgcttaacg
    13261 ttattatgga atcacaaccc cctttccctg tactaaaaac caattagtct tcctttgctt
    13321 cctcctcgac ctcttccttc tcagcatatt ttgcattaat ttcttcaatc cttctgatga
    13381 tctgttcctt ctcggcgtcc atgcgctctt ttatctgatt cactgcatta tccaaactgc
    13441 tttttgcatt ctcgatcatc tcttcaactt ccagcttcat gtctctaatg atcttgttca
    13501 aatccacaat caacatgcgc ttttcatctg cctgaattga aagtctttcc ctttcctttg
    13561 cagattcctc ttgagctttg gcaatgatat tctttgcagt gttctccgca tttattaaaa
    13621 catctgctat ttttgttttt tcttcctggg cttgaatata tgctgtggac agttcattga
    13681 gttttgatgc aagctgttcg ttctttgctc tgagctcgtc gcattctttt gccttttcac
    13741 ttagagactg ctgatattga gatactagct tctctatgta ttcgttaacc tgcttcttgt
    13801 taaaaccgct aaattccgta tcaaatactt tattgccttt tgccattatg ttctcctcct
    13861 gccagctctt aaaattgtta cagttgtgta taattctact tattcagtat attgtattgg
    13921 ctatctttcg tcaagttcaa aatatgccat ttggatgcac taccaccttc ttttgacata
    13981 aggaaaaggc gtctccgaag gcgcaggact ttgagatggc tccggtgt
//
LOCUS       DAONVY010000004        13672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_3432_length_13672_cov_3.934494, whole genome shotgun sequence.
ACCESSION   DAONVY010000004 DAONVY010000000
VERSION     DAONVY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13672
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_13672_cov_3.934494"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLI11_00225"
     CDS             complement(<1..991)
                     /locus_tag="PLI11_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HPZ51325.1"
                     /translation="MVMNKAILHVLDFVSGVFVFSEKEMDVSNEHVFKFLEKHVQRSL
                     QDAGRKEGRFLEESEMWRMIVEYVNGELDFITLSQVVANQMHDNISNSSLPVSTDVLV
                     ADVLLDDRKYLCMLFFENKTAFTHNVISDEYGVRGEIVKHYSILPGATQKLSEYAIVC
                     LDDFSICFAEKKRNIDGKDTFVLSERILMCASSLSTKEAVKMVNKIVAEVTDEFGGDA
                     TKTLTKAKAYIAENLEESETLDPIDLGRNVFSGNETMMEEFESKAKEYGFPEVVKVDR
                     DYVKRSVHVHKIKTDTGIEITIPNTCFNNPEYVEFVNNEDGTISINLKNIGKIT"
     gene            complement(994..1572)
                     /locus_tag="PLI11_00230"
     CDS             complement(994..1572)
                     /locus_tag="PLI11_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ51326.1"
                     /translation="MQLKMWWDNTGKEPVVKLPEGFYIRSYEGEKDVFKWVEITSVDL
                     QNTIITVEDFERDMLNREGVQKEHIYFICFDGEEVGTVTAIMYPEKKQGYVHMVAVKK
                     EFRGRGLGSALTNLALKVFFDNGMENAYLKTDDFRVPAIRSYLKAGFKPMLYHEDMEA
                     RWRSIMEVCNLNSLDCVDEKGIYLKTIKRDEE"
     gene            1740..3659
                     /locus_tag="PLI11_00235"
     CDS             1740..3659
                     /locus_tag="PLI11_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPZ51327.1"
                     /translation="MKSIKTKLVLLLSLFILTSSISIGITSIRASTRTAVKEAEKTLS
                     SIARESAKLTKSRIDSQKMIVEMLAINEDIKSMDWEKQRSVLQKYAKETSVLDLAIVH
                     LDGTAYYPDGSTSQLGDREYVQKAFQGKANVSDLLVSRVTNNVVLMFAAPIINDGKVD
                     AAVIARMDGNTLSDLASDTGFGKDGYGYLVNDKGTVVGHPDRDLVLSQFNPLEESKVD
                     ESYKPLASVVKYIIEKKSGVTSYSHDGMALYASFAPVEDSNWFFVITASEDEVLSSLH
                     SLQLYISIIVTVILLTAMTLAFFLGRTIAKPIIEAVKYSENIANLNLSTDVPTKLQEK
                     KDEIGVLARAIQTVIDNLNKIMQEINVSSENVANGARLVSESSLELSQATTEQASSVE
                     ELSASLKEISVHTKHNANKANEANALASNTRNNALNCNEKMTRMLEAMEEINVSSNNI
                     SSIIKVIDDIAFQTNILALNAAVEAARAQEHGRGFSVVAEEVRNLAQRSAQAAKETSN
                     LIKNSIRKAENGAKIAEETAVELNKIVEDITRVAELVKSIADASNEQSMGIAQISEGI
                     FQVSQVIQSNTATSEEGAAASEHLSNQADFLRNTVARFKLKEMKMDNDAMPKEEIAAK
                     PVNNKTYRNTDFGKY"
     gene            complement(3656..5257)
                     /locus_tag="PLI11_00240"
     CDS             complement(3656..5257)
                     /locus_tag="PLI11_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51328.1"
                     /translation="MKYSIKGYMEFVKGFYKDMSKPRRILFTLLCICLSFYISAVTFL
                     AYSEIHVASLLLFFIYAFVFFNLLVFFCHKLKNKYIKVNAEKSKLNKRTFFFCSLICF
                     VILFVTFLAYFPGGISPDNVSQWKQIQSFEFDNWHPVFHTFLMYLTTRIVNSYPFVIF
                     VQIVFFSIAMGYLLSTLESWGFSKKVLMTVELFVVLNPLVRNLLMYGWKDAALTIFLM
                     FLFSFVTNIYFTDGLWFSRMRNVVLLSVLTAFATLVRHNAFFYTLPLVLFIVLFYFKK
                     SKKTIFLPAAVLAIYFLVVGPFYNMLNVTKPDNVFSESVGIPMTIMGDVMKKNMEVLP
                     EKTRNFLLDIAPAEKWEEVYECGNYNSIKFESMHQEVVNNISAREFFVMTLDTIKRDP
                     KNSFLAVRELTGFVWKVSGEGARINVVTNGEEIGYTYKNTFINRFCMFFVRIVDMAIN
                     IPVMETIFALFGVQMLVLMMVGLFSIQRNGAKTLMFVIPNLAYNLGTMLLLCGKDYRF
                     FHFNLVLTTSACLVMLARQADVIKD"
     gene            complement(5264..5545)
                     /locus_tag="PLI11_00245"
     CDS             complement(5264..5545)
                     /locus_tag="PLI11_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51329.1"
                     /translation="MELHDILETLMIVCFGVSWPMSIIRSVRSKSTKGKSLLFMCFIA
                     LGYLCGIISKLMSHTYNLAFWFYFPNIIMVLTDIALYFRNRRYEKLKGE"
     gene            complement(5563..6939)
                     /gene="fumC"
                     /locus_tag="PLI11_00250"
     CDS             complement(5563..6939)
                     /gene="fumC"
                     /locus_tag="PLI11_00250"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPZ51330.1"
                     /translation="MEYRVEHDSMGEVRVPADKYWGAQTQRSLVNFRIGVGLELMPKE
                     IIYAFAIIKKACAVANHEQNPTRMTQEKMEVICNVCDEIMEGKLDEHFPLVVWQTGSG
                     TQTNMNVNEVIANRGNELCGKKLLHPNDDVNMSQSSNDTFPTAMHIAAVLGIEDRLLP
                     ALNKLIGTFKRLEKENEGIVKTGRTHLQDATPIAFSQEISGWRYSLEKAKEMMENSLT
                     GLCDIALGGTAVGTGLNAPKGFDVEVAKQISEITGKKFRTSQNKFHSLTSKNEIVNAH
                     GAIKALACDLMKIANDVRFLASGPRCGLGEISIPENEPGSSIMPGKVNPTQCEALTMV
                     AAQVMGNDTTITIAASQGNFQLNVYMPVCIYNFLQSVRLLSDAIVSFNDNCASGIKAN
                     KDKMRSNLHNSLMLVTALNPYIGYENAARTARLAYEENISLKEACLKLGFLTEEEFDK
                     VFKPEDMI"
     gene            complement(6995..8131)
                     /locus_tag="PLI11_00255"
     CDS             complement(6995..8131)
                     /locus_tag="PLI11_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPZ51331.1"
                     /translation="MNRYVVAFFLFSFLGWVWESIYCTLRDKKWANRGFLYGPVCPIY
                     GFGSLACFLMHDLTEKGYLPEPSWWVVFLLGFATSMILEYFTSWLLEKLFNARWWDYS
                     TLPLNLKGRSSIPTSVAFGVGAIVLMKGLIPALYLFLEKAPKPMLDAFSLLFVALLSI
                     DTALTVSSLTNFQKQVTAIEEGFQNRMTNIVDKLFGYKENLYNKSVQRIRIFKFPGHR
                     NRIARQLREQRFEELVREYFEHEIVLQMDKYIQHGNTTTLEHCENVAWISYLINQKLN
                     LNADEKALVEAAILHDLFQYDWHEEDSERKLHGFHHAEIACNNAVKYFNISEKERHAI
                     RSHMWPLNITRIPKNKEALIICIADKYCALVETIRLNKRLGLRK"
     gene            complement(8233..8919)
                     /locus_tag="PLI11_00260"
     CDS             complement(8233..8919)
                     /locus_tag="PLI11_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPZ51332.1"
                     /translation="MKLVYKGKTKDVYELEDGNILLKFKDDATGEDGVFDPGANAVGL
                     TIEGAGLAGLRMTKYYFEKLNEMGVPTHYISADLDDVSMKVKKATPFGDGLEVICRYR
                     AVGSFIRRYGKYAKEGQVLDGFVEFTLKDDLRNDPPIGKDALVMLGIMTQEEYDTLKG
                     LTKRISDIIKQDLAERGIELYDIKLEFGRVGEDRHIALIDEISGGNMRAYKDGKYVEP
                     LELAQLVTGK"
     gene            complement(9037..10722)
                     /locus_tag="PLI11_00265"
     CDS             complement(9037..10722)
                     /locus_tag="PLI11_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ51333.1"
                     /translation="MKFKGIMAFVIGLCLLVSLIQYQPSSAESGAKLAFSSVSANYFA
                     GGPNFGTKLSNAYDGDPSTFANYAAHKAGDRYREGTYFLFDLGQVCDVSKITVIFANP
                     VRTHNFELYTSVDGISYEKIFEFTDKEYGANGSEFNIVKNIQARYIKLMALGAYVNES
                     RDLNENYHLAEVAVYGKVADVVTANRIEVADVVAKNFASGPNFGQVLDHLTDGNPTTS
                     ANYAPHKGDGQYKNDPHFLIDLGDLYELEKVALITAHPSLGNKRIICFDIYISQDNEN
                     FEKVYSFFNKTSYGHDGYFNIPVEGVARYIKLSATAAFDNGTTLSDNYQVYEFAAYGT
                     EYVEPATEPTTEPTTEPTPEPTAEPTTEPTTEPTIEPTSEPDNMITIVSGEMHGFSDN
                     SPVEGTKIENAYDGNDATLVKVIGHVGGTGNHLGDNAYFTFDLGGVYELGGIRLNFGT
                     TRAHFYKILVSVDGENYTEAKSHTSAYEKTGEYYIRNEELGGVKARFVRIVAYGTVAS
                     PGNQYYMLSEISVFGTEAADENPQTSDVFLLLPIMVVLSLSVVFFSRKRRVMV"
     gene            10952..11845
                     /locus_tag="PLI11_00270"
     CDS             10952..11845
                     /locus_tag="PLI11_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51334.1"
                     /translation="MKRFIILSIVLISLVFFGCQKDNEKDPSPTPSPTETEKVDKTDA
                     PTPEPTEEKTPEPTKEPLSEKQLGIPQDDAEIIYLDDFVDNYINPEALFRYNNGHEVV
                     EEQLYLSTYKGVPVELVDTYSPDYYCEAKVDGISQVQFHLRFKTSHERAEKDPWMAAI
                     IGVRVYEPMGNSFKPADEDSGIYVAVTQINKILVYHGVPGHWPRGAFSMEIPNGFGEM
                     CELVIVDTGDKLFYYQKLEGSEPTLFMIIELSDQIKCFDADGNEVYSADNNLKEDKGG
                     YFKIFNHFGRTVIDEFSIKVR"
     gene            11851..>13672
                     /locus_tag="PLI11_00275"
     CDS             11851..>13672
                     /locus_tag="PLI11_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51335.1"
                     /translation="MTKNTLIKIVCFFIAITLLITACSKNNNGNNSDNNKTESKEALE
                     LSVYAGVDDLGRVLPLAGDNGVPAYDSNKYVGIFYFVWHGARQVPGPYDVSKIIEKDK
                     DAARSDEAWQAAGGGPVGTNHWWGEPLFGYFRNTDRWVMSKDVQMLTDAGVDFIIFDY
                     SNGNSYTQAVLTMLEILDTFHKQGFNVPKVSFVTKAESVRHMKEIYNDIYKSHPEYEH
                     LWFKLDGKPLMIGSNKSPDLDQEVKDFFTIKYAQWPREPYSDDGFPWMDFQRPQTLYG
                     KESGTTIMSVSVAQHSGTLALSSSAFYGDDTNKTRSYHNGANDKSEDAYLYGYNFAEQ
                     FEYAISQNPDIIFVTGWNEWIATRQNPGSWKYIDGTPNYEPVILVDAADINNSRDIQP
                     MQGGFGDNYYMQLISYIRKFKGLAPYNTNLPEKSTTYGTIDFSNGFADWDNVGQTYID
                     YTQDTEHRDARGYGRIVYKNDTGRNDIFVSKVACDNKNIYFYVETLDPLTPKEDKHWM
                     NLFISTGNEGNKTWHGYDFVVNRVSPSDNKAVLEKCFGGWNFEKVQDIDFMYNNNKLQ
                     IAIPLETLGYESHENLRIEFKWADNYVLNENNEADIFTFYT"
BASE COUNT         4223 a         3067 c         2526 g         3856 t
ORIGIN      
        1 tcgttatctt tccaatattc tttagattta ttgaaatggt tccgtcctca ttgtttacaa
       61 actccacata ctcagggttg ttgaagcaag tgttgggaat ggttatttca atacccgtat
      121 ccgtctttat cttgtgcaca tgcacgcttc tttttacata atccctgtcc actttgacaa
      181 cttcaggaaa gccgtattct ttcgcttttg actcaaattc ctccatcatt gtttcgtttc
      241 cggaaaacac attccgcccc aaatctatag gatcaagagt ttcgctttcc tcaagatttt
      301 ctgcaatata tgctttggct ttagtaaggg ttttagtagc gtctccacca aactcatccg
      361 taacttccgc cactattttg ttgaccattt tcacagcttc tttcgtggac aaagaagatg
      421 cacacataag tatcctttca gacagcacga aagtatcctt tccatctatg tttcttttct
      481 tttccgcaaa acatatggaa aagtcatcaa gacatacaat tgcatactca ctcagcttct
      541 gtgttgcacc aggaagaatt gaatagtgtt ttacaatctc tccccttacc ccgtattcat
      601 cactaattac attatgggta aaagctgtct tgttttcaaa aaacagcatg cagagatatt
      661 ttctgtcatc aagaagcacg tccgccacca ggacgtccgt ggaaacaggg agactggagt
      721 ttgaaatatt gtcatgcatt tggtttgcaa caacttgcga cagtgtaata aagtcaagct
      781 ccccatttac atactcaaca atcattctcc acatttcgct ttcttcaaga aagcgtcctt
      841 cttttcttcc cgcatcctgc agtgaccttt gaacatgttt ttccagaaac ttaaacacat
      901 gttcattcga aacatccatt tccttctctg aaaaaacaaa cactcccgaa acaaagtcaa
      961 gaacatgcaa tatcgcttta ttcataacca tacctattcc tcatcccttt ttatagtttt
     1021 caagtaaatg cccttttcat cgacacaatc caggctgttt aaattacaca cttccattat
     1081 gcttctccac cttgcttcca tatcctcatg gtacagcatg ggcttgaaac ctgcttttaa
     1141 ataacttctt atggcaggca ccctaaagtc atccgttttt agataagcgt tttccatgcc
     1201 gttatcgaaa aatactttca gagcaagatt cgtgagtgca gaaccaagac ctctccccct
     1261 gaactccttc tttacagcca ccatatgtac atacccttgc tttttctccg ggtacataat
     1321 ggcggtaact gtaccgacct cttcaccatc aaagcatatg aaatagatat gctctttttg
     1381 caccccttca cggtttagca tatccctttc aaagtcttcc acagtaataa ttgtgttctg
     1441 caggtcaaca ctggtaatct caacccattt gaaaacatcc ttctcacctt catagcttct
     1501 tatgtaaaag ccttcaggca gttttacaac aggctccttc cctgtattgt cccaccacat
     1561 tttcagctgc ataccaatcc tctctcttca aatcaaaatt attcaaaaga gattatacat
     1621 cacccaacca agttctgcaa aaggcaagtt ccctaaagtt tttcatggat atgtcgataa
     1681 tatacatggt ttattgactt tttaggcaat aactaaattt tgagaggagt atttacttga
     1741 tgaagagcat aaaaaccaaa ctggttctgt tactatcttt atttatccta acctcatcca
     1801 taagtattgg aataacgagc attagggcat caaccagaac cgcagtaaaa gaagcagaga
     1861 aaaccctttc atccattgca agagagtctg caaaactgac aaaaagcaga atagactcac
     1921 agaaaatgat agtggaaatg ctggcaataa atgaggatat aaaaagtatg gactgggaga
     1981 aacaaaggtc agtcctgcag aaatatgcaa aagaaaccag tgtgctggac cttgccattg
     2041 tccacctgga tggaactgca tactaccctg acggaagcac cagtcagctt ggagacaggg
     2101 aatacgtgca aaaggctttc cagggcaagg ccaatgtctc cgaccttctg gtaagcagag
     2161 tcacaaacaa tgtggttctc atgtttgcag caccaattat caatgatggc aaggttgatg
     2221 ctgcagttat tgcacgcatg gatggcaata ccttgagcga tttggccagt gataccggct
     2281 ttggaaaaga cggctatggt tatttggtta atgacaaagg tacagttgta ggtcatccgg
     2341 acagggacct ggtattgagc caattcaacc ctctggaaga aagcaaagta gacgaaagct
     2401 acaaacccct tgcctcggtt gtcaaatata taatcgaaaa gaaaagcgga gtaaccagct
     2461 attcccatga tggaatggct ctttacgcaa gctttgcacc tgtggaagac agcaactggt
     2521 tttttgtaat tacagcaagt gaggatgagg ttttgtccag tcttcactca ctccagctgt
     2581 acatatcaat tattgtgaca gtcattctgc ttactgccat gactttggcg tttttccttg
     2641 gcaggacaat agcaaaacca attattgaag cagtgaaata ctcagagaac attgcaaacc
     2701 ttaacctcag cacggatgta cccacaaagc tccaggagaa gaaggatgaa ataggggtac
     2761 ttgcaagagc aatacagacc gtgattgata atctcaacaa gattatgcag gagattaacg
     2821 tatcttctga aaatgttgcc aatggcgcaa gactggtttc cgaatccagt ctggaactat
     2881 cacaagcaac caccgagcag gcaagttccg tggaggagct ttcagcttca ttgaaggaga
     2941 tctccgtgca cacaaaacac aacgccaaca aggcaaacga ggcaaatgcc cttgcttcaa
     3001 acaccagaaa caatgcatta aactgcaacg agaaaatgac caggatgctg gaagccatgg
     3061 aagagataaa tgtgtcctcc aataacattt caagtattat aaaagtaata gatgatattg
     3121 cgttccagac aaatatcctt gctctcaatg ccgcagtgga ggcagcaaga gcccaggaac
     3181 acggcagagg tttttccgta gttgcagaag aagtcagaaa tcttgcacaa aggtctgcac
     3241 aggctgccaa ggaaacttcc aaccttatca aaaactccat aagaaaagct gagaatggag
     3301 cgaaaattgc cgaagaaacg gcagttgaac tgaacaaaat tgtggaagac ataaccagag
     3361 tggctgaact tgtcaaaagc atagccgatg catcaaacga acagtcaatg ggtattgcgc
     3421 aaatcagcga aggaatcttc caggtgtctc aggttattca gtcaaataca gccacctctg
     3481 aagaaggagc tgcagcaagt gagcacctgt ccaatcaggc ggatttcctg cgcaacactg
     3541 ttgcaagatt caagttaaag gaaatgaaaa tggataacga cgcaatgcca aaggaagaaa
     3601 ttgcagcaaa gcctgttaac aacaaaacat acagaaatac tgatttcgga aaatactaat
     3661 cttttatgac atcagcctgc ctggcaagca ttacaaggca ggctgatgtt gtaagtacaa
     3721 gattgaaatg aaagaacctg taatctttac cgcaaagaag cagcattgtt cctagattgt
     3781 aagcaagatt gggaatcaca aacattaagg tttttgcccc gtttctttgt atggaaaaca
     3841 gaccaaccat cattaaaaca agcatctgaa ctccaaaaag agcaaaaatt gtttccatga
     3901 caggaatatt tattgccata tccactatcc ttacaaaaaa catgcaaaac ctgtttatga
     3961 atgtattttt gtatgtataa cctatctctt ctccattggt tacaacattt attctcgcac
     4021 cctccccgct taccttccac acaaaacccg taagctccct gacagcaaga aaagagtttt
     4081 tgggatccct tttgattgta tcaagggtca tcacgaaaaa ctccctggcg gatatattgt
     4141 taaccacttc ctgatgcatg ctctcaaact tgatagagtt gtaatttcca cattcataaa
     4201 cttcttccca cttttcagca ggtgcaatat ccaaaagaaa gtttcttgtt ttctcaggca
     4261 gcacttccat gttcttcttc atcacatccc ccattatggt catgggtatg ccaaccgatt
     4321 ctgagaatac attgtctggt tttgttacat ttaacatgtt gtaaaaagga ccaacaacaa
     4381 gaaaatatat ggcaagtacc gcagcgggaa gaaaaattgt cttctttgac tttttgaaat
     4441 agaaaaggac aataaacaaa acaagaggca gcgtatagaa aaaggcatta tgtcttacca
     4501 atgttgcaaa tgcagtcaaa acagacagca aaacaacatt tctcatcctg gaaaaccaca
     4561 gcccgtcggt aaaatagatg tttgttacaa aagaaaataa aaacataagg aaaatggtca
     4621 aggctgcatc cttccagcca tacataagaa gatttctaac caaaggattc aaaacaacaa
     4681 acaattcaac agtcatcaga actttctttg aaaaacccca ggactccaga gtcgaaagca
     4741 aatatcccat ggctatactg aaaaacacaa tttgaacaaa tatgacaaag gggtaagaat
     4801 taacaattct tgttgtcagg tacatcaaga atgtatggaa aactggatgc cagttgtcaa
     4861 actcgaagga ctgtatctgc ttccactggg acacattgtc tggcgagatt cctcctggaa
     4921 agtatgccag aaatgtaaca aacaggatga caaaacagat caatgagcaa aaaaagaaag
     4981 ttcttttatt caattttgat ttttctgcat ttaccttgat atatttgttt tttagtttgt
     5041 gacaaaagaa cacaagcaga ttgaagaaga cgaatgcgta aataaaaaaa agcaataaag
     5101 aggctacatg gatttcactg tatgcaagaa aggtaaccgc ggatatgtaa aatgaaagac
     5161 agatgcaaag aagtgtaaac agaatccttc ttggctttga catatccttg taaaaacctt
     5221 tcacaaactc catgtatccc tttattgaat atttcattta aatctattct cccttcagtt
     5281 tctcatatcg tcgattcctg aagtacaatg cgatatccgt caacaccata atgatattgg
     5341 gaaaataaaa ccaaaacgcc agattgtatg tatgcgacat cagctttgat attataccac
     5401 acaaataccc caatgcaata aaacacatga ataaaaggct tttgcctttt gttgattttg
     5461 agcgtactga tctgataatc gacataggcc aggatacacc gaagcagacg atcatcagtg
     5521 tttcaagaat atcgtgcaat tccatttaat ctccaatcct ttctaaatca tatcttcagg
     5581 tttgaacacc ttgtcaaact cctcttctgt aaggaaaccc agcttcaagc atgcttcttt
     5641 tagtgaaata ttctcctcat aggcaagtct ggcagtcctg gctgcattct catatcctat
     5701 ataaggatta agtgcagtaa ccagcattaa agaattatgc agatttgatc tcatcttgtc
     5761 cttgtttgcc tttatgccgg aggcacaatt atcattgaaa gaaacaattg catctgacaa
     5821 aagccttacc gactgtaaaa agttatatat acataccggc atgtaaacat taagctggaa
     5881 gttcccctgg gatgcggcaa tggttattgt ggtgtcgttg cccataactt gtgctgccac
     5941 catggttagt gcttcacact gggtgggatt tacttttcca ggcatgatag atgaacctgg
     6001 ttcattctcc ggaattgaaa tctcgccaag accgcatctt ggaccactgg caaggaaccg
     6061 tacatcgttt gcaattttca tcaaatcaca agcaagagct ttaatggcgc catgggcatt
     6121 gacaatttca tttttggaag ttagggaatg aaacttgttt tggcttgttc taaacttttt
     6181 tcccgtaatc tctgaaattt gctttgccac ctccacatca aatcctttgg gagcattcaa
     6241 acctgttcct accgcagtgc cacccaaagc gatatcacaa agcccggtca aagagttttc
     6301 catcatctct tttgccttct caagactata tctccacccg cttatttcct gggaaaaggc
     6361 aatgggcgtt gcgtcctgca gatgagtcct tcccgtcttg actatgcctt cattctcttt
     6421 ttcaagtctt ttgaacgttc caatcagttt gttcagtgcc ggcaaaagcc tgtcttcgat
     6481 ccccaggaca gcagcaatat gcatagccgt tggaaatgta tcgttggatg actgactcat
     6541 gttgacatca tcatttggat gaagaagctt ctttccacat aactcgtttc ccctgtttgc
     6601 aattacttca tttacattca tatttgtctg ggtgccgctt cctgtctgcc ataccaccag
     6661 cggaaagtgt tcatccagct ttccttccat aatctcatcg caaacattgc agataacttc
     6721 catcttctct tgggtcatcc tcgttgggtt ttgctcatgg tttgccaccg cacaagcctt
     6781 cttgattatg gcaaaagcat atattatttc ttttggcata agttcaagac caacaccaat
     6841 tctaaaattc acaagacttc tctgggtctg ggctccccag tatttatccg ctggaaccct
     6901 tacctctccc atcgaatcat gttccactcg atactccatc cttccctact tcctttctgt
     6961 tttcttccta ccatatagtt atatcaaaac tcaatcattt ccgcaatcca agacgcttgt
     7021 taagccttat cgtctcaaca agagcacagt acttgtccgc aatacagata atcagtgctt
     7081 ccttgttctt cggtatccta gttatattta gaggccacat gtggctcctt atggcgtggc
     7141 gttccttttc ggatatgtta aaatacttca cagcgttgtt gcatgctatc tccgcatgat
     7201 gaaagccatg cagttttcgt tcactgtctt cctcatgcca gtcgtactga aacagatcat
     7261 gaagaatcgc agcctcgaca agcgcctttt catccgcatt caagttcaac ttttggttta
     7321 taagataact gatccatgcc acgttttcgc aatgctcaag agttgtcgtg tttccatgct
     7381 gtatgtattt gtccatctgc agcactattt catgctcaaa gtattccctc acaagctcct
     7441 caaaccgctg ttccctcagc tgtcttgcta tacggttcct gtgaccagga aacttgaaaa
     7501 tccttatacg ctggactgat ttgttgtata gattttcctt gtacccgaac aacttgtcca
     7561 caatgtttgt catgcggttc tgaaaacctt cctcaatggc agtaacctgc ttctgaaagt
     7621 ttgtaagaga tgaaactgta agtgccgtat ctatggataa aagggcaaca aacaaaagag
     7681 aaaaagcatc cagcattggt tttggtgctt tttcaagaaa taaatacagc gcaggaatca
     7741 aacccttcat cagcacaatg gctccgacac caaacgcaac agaagtaggt atactgcttc
     7801 ttcccttcag attcaatgga agagtactgt agtcccacca tctagcattg aacagctttt
     7861 caagaagcca ggaggtgaag tattccaata tcatgcttgt tgcaaaacca agaagaaaaa
     7921 caacccacca gctgggttca ggcagatagc ctttctcagt aagatcatgc ataagaaaac
     7981 atgccagtga accaaatcca taaatcggac aaacaggtcc atataagaac cctctgttgg
     8041 cccacttttt gtccctcaag gtacagtata tgctttccca tacccatcca agaaaactga
     8101 aaagaaaaaa agcgacaaca tacctgttca tcgtaccaac ttcccttcgt tttcacattt
     8161 gcggttaaaa atatatttca gcaagaaatg tttgacaagt cgtcataaag tcaaaagaac
     8221 ctaacaaggg ttctatttgc ctgtcacaag ctgcgcaagt tccaaaggtt caacatattt
     8281 tccatccttg tacgcgcgca tgttgcctcc tgatatttca tctatcaaag caatatgtct
     8341 gtcctctcca actcttccaa attccagctt tatatcgtaa agctcaatac ccctttctgc
     8401 caggtcctgt ttaataatgt cggatattct ttttgtaaga ccttttaaag tatcgtattc
     8461 ctcctgagtc atgataccca gcattacaag ggcatccttt ccaattggag ggtcgtttct
     8521 taaatcatcc ttcaaggtaa actcaacaaa accgtcaagc acttgtcctt ctttggcata
     8581 cttgccatat cgtcttatga agctgcctac cgcacgataa cggcatatga cttcaagacc
     8641 atctccaaaa ggtgttgcct tttttacctt cattgatacg tcatccaagt cagctgatat
     8701 atagtgagtg ggtactccca tctcattcag cttctcgaaa tagtatttag tcatcctaag
     8761 tccggcaaga cctgctcctt caattgtcaa gcctactgca tttgctcccg gatcaaacac
     8821 accatcttca ccagttgcat cgtccttgaa tttcaaaagt atgtttccat cttcaagttc
     8881 gtatacgtct tttgtctttc ccttgtacac cagcttcata acacaaatcc tttctgttaa
     8941 tttgtatacc ctagtatatt atcactaaaa aggaaaaaaa taaatacatg cccaaccaat
     9001 tttgtcaggc atgtatttat caattttcct ataaccttat accattactc ttctttttct
     9061 tgaaaaaaaa acaactgaca aggacaaaac caccataatg ggaagtagta aaaatacatc
     9121 acttgtttga ggattctcat cagcagcttc agttccaaat actgatatct cacttagcat
     9181 gtagtattga ttgcccggag atgcaactgt tccgtatgcc acaatgcgta cgaatcttgc
     9241 cttgacacca ccaagctcct catttcttat gtaatactcc cctgtctttt catatgcact
     9301 ggtgtgactc tttgcttctg tgtaattctc accatccaca ctaacaagaa tcttgtagaa
     9361 atgggctctt gttgtaccaa aattgagcct tataccacca agctcgtaca caccaccaag
     9421 gtcaaaggtg aaatatgcat tatcaccaag atgattgcct gttcctccaa catgaccaat
     9481 aacctttacc agtgttgcgt catttccgtc gtatgcattc tctattttcg ttccttcaac
     9541 aggactgttg tctgagaatc cgtgcatttc cccggaaaca atggttatca tgttgtctgg
     9601 ttccgatgtt ggttctattg taggttcggt cgtaggttct gttgtgggtt ccgccgtcgg
     9661 ctcaggtgta ggttctgtgg ttggctctgt ggttggttct gtggcaggtt ccacatattc
     9721 agtaccgtaa gcagcaaact cataaacctg atagttgtca gacaaggtag ttccattgtc
     9781 gaaggccgca gtggctgaaa gctttatgta tcttgcaact ccctcaacag gtatgttgaa
     9841 atatccatcg tgtccataac tggtcttgtt aaagaagcta taaacctttt caaagttctc
     9901 attgtcctgg cttatgtata tgtcaaaaca aattattcgc ttgttcccaa gagaaggatg
     9961 agctgttatc aaagctacct tttccagctc gtacaaatca ccaaggtcaa ttagaaaatg
    10021 tggatcattc ttgtattgac catctccctt gtgaggagca tagtttgcac tagttgttgg
    10081 atttccatcg gtaagatgat caagaacttg tccaaagtta ggaccgctgg caaagttctt
    10141 cgcaaccaca tctgccactt caatcctgtt ggctgtcacc acatcggcca ctttcccgta
    10201 tacggcaact tctgcaagat gatagttttc gttcaaatct ctactttcat taacataagc
    10261 tcctagagcc atcagcttta tgtatcttgc ctgaatgttc ttaacaatat tgaattcact
    10321 gccatttgca ccgtactcct tgtctgtaaa ttcaaatatc ttctcatatg aaataccatc
    10381 aacacttgta tatagctcaa agttgtgagt tctgacagga tttgcaaata ttacagtaat
    10441 tttgcttaca tcgcagactt gtcccaaatc aaaaagaaaa tacgtacctt ccctatacct
    10501 gtctcctgcc ttgtgagctg catagtttgc aaaggttgaa ggatctccgt cgtatgcatt
    10561 tgacagtttt gttccaaagt ttggaccacc tgcaaaataa tttgcagata ctgaagaaaa
    10621 cgcaagtttt gcaccacttt ccgcactgga tggctgatat tgtataaggc tgaccaaaag
    10681 acacaaccct ataacaaatg ccattatgcc tttaaacttc atataaaccc ctctttcata
    10741 aatggaatct gtttattaat attctaataa cagaaatgtt ccaatcatat aactttattg
    10801 atttcattgg ataggaataa taatatattg atttgaaacg tcaattcaat caaaattatt
    10861 tcaaaaactt gaaaacaaat caacatacta atattatctt cagggcaatc aatataatat
    10921 atattcacaa aggattttgg gaggtaaaat catgaagaga tttattatac tgtctatagt
    10981 tctgatctca ctggtcttct ttggatgcca aaaagataat gaaaaagacc cttcacccac
    11041 tccatctcca actgagacgg aaaaggtaga taaaacagat gcaccaacac ctgaaccaac
    11101 agaagaaaaa acacctgaac caacaaaaga accattgtca gaaaaacaac tgggtatacc
    11161 tcaggatgat gcagaaatca tatatctgga tgattttgtt gacaattaca taaaccccga
    11221 agctcttttc agatacaaca acgggcacga ggttgttgaa gaacaacttt acctgagcac
    11281 atacaaagga gtacccgtag agttggtaga cacatattca cccgactatt actgcgaagc
    11341 aaaagtagac gggatttcac aagtccagtt ccatcttagg tttaagacct ctcacgaaag
    11401 agcggaaaaa gacccttgga tggcagcaat aataggtgta agagtgtatg aacccatggg
    11461 aaacagtttc aaacctgccg acgaagattc aggcatatac gtggctgtta cccagatcaa
    11521 caaaattctt gtttaccacg gtgtacctgg ccactggcca agaggtgcct tcagcatgga
    11581 aataccaaac ggttttggcg agatgtgcga gcttgttatc gtggatacag gagacaagct
    11641 gttctactac cagaagctgg agggctctga accgacattg ttcatgatca ttgaactttc
    11701 cgatcaaatc aaatgcttcg atgcagatgg caatgaagta tactctgccg acaacaacct
    11761 caaggaagac aagggaggat acttcaaaat ctttaatcac tttggaagaa cagtaattga
    11821 cgaattctct ataaaggtgc gttgattgat atgacaaaaa atacactaat taaaattgtc
    11881 tgctttttta tagccataac actactgata accgcctgca gcaaaaacaa caatggcaat
    11941 aacagcgaca ataataaaac agaatcaaag gaggcgcttg aactgtcagt atacgctgga
    12001 gttgacgatt taggaagggt gctacctctt gctggggata atggtgtccc tgcttatgac
    12061 agcaacaaat atgtaggaat cttctatttt gtttggcatg gagcaagaca ggttccagga
    12121 ccctatgatg taagcaaaat tattgaaaag gacaaggatg cggccagatc cgacgaagca
    12181 tggcaagcag ctggtggcgg tcctgttgga acaaaccact ggtggggaga accgttgttt
    12241 ggctacttta ggaacacaga caggtgggta atgtcaaagg acgttcagat gcttaccgat
    12301 gcgggcgttg attttataat ctttgactat tccaacggaa actcatatac ccaagcagta
    12361 cttacaatgc ttgaaatatt ggacaccttc cacaagcaag ggtttaatgt tccaaaagta
    12421 tctttcgtta caaaagccga gtccgttcgt cacatgaagg aaatctacaa cgacatatac
    12481 aagtcccatc cggagtatga gcacctttgg ttcaaactgg acggcaagcc tttaatgata
    12541 ggcagcaaca agtcacccga tctggaccag gaagtaaagg acttttttac cataaagtac
    12601 gcccagtggc caagagagcc atacagtgat gatggtttcc cctggatgga tttccaaaga
    12661 ccacagaccc tgtatggaaa ggaatcggga acaaccataa tgagcgtatc tgtggcacag
    12721 cactcgggaa ctcttgccct aagttccagc gctttctatg gcgatgatac caacaagaca
    12781 agaagctacc acaacggcgc taatgacaaa tccgaagatg catacttgta cggttacaac
    12841 tttgcagaac agtttgaata tgcaataagt cagaatcccg atataatatt tgtcactggc
    12901 tggaatgaat ggatagcaac aaggcagaat ccaggtagtt ggaaatatat cgacggcaca
    12961 ccaaactacg agccagttat tcttgtagat gcagctgaca tcaacaactc aagagatatc
    13021 cagccaatgc agggaggttt tggcgacaac tattacatgc agctcataag ctacataaga
    13081 aagtttaaag ggcttgcgcc atacaataca aatttgcctg aaaagagcac aacctacgga
    13141 acaatagatt tttcaaatgg ttttgcagac tgggataatg ttggtcaaac atatattgac
    13201 tacacccagg atacagaaca cagggatgca agaggctacg gcagaattgt gtacaagaac
    13261 gatacaggca gaaacgacat atttgtaagc aaggtggcct gcgataacaa aaacatatac
    13321 ttctacgtgg aaacattaga cccactgact ccaaaggaag acaaacactg gatgaacctc
    13381 tttatctcca cgggcaacga aggcaataag acctggcatg gttatgattt tgtcgtaaac
    13441 agggtttctc catctgataa caaggcggta ttggaaaaat gttttggcgg atggaatttt
    13501 gaaaaggtac aagatattga tttcatgtac aataacaaca aacttcaaat tgccattccc
    13561 cttgaaactc taggatacga atctcacgag aatctgcgaa tcgagtttaa gtgggctgac
    13621 aattacgtat tgaacgaaaa caatgaagcg gacatcttca ccttctacac tg
//
LOCUS       DAONVY010000005        13206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_3594_length_13206_cov_4.561630, whole genome shotgun sequence.
ACCESSION   DAONVY010000005 DAONVY010000000
VERSION     DAONVY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_13206_cov_4.561630"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLI11_00280"
     CDS             complement(<1..1054)
                     /locus_tag="PLI11_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51336.1"
                     /translation="MENKKGLLIITTISMLILLTVAILFPRKPAEAENIQYINAMEKI
                     SDTQLGKLSPTAQVDMSVFLDTAIPYLTFEHGEDASKVALKHGYVKSIGKEVLRRNDA
                     LDILFKITGVKLPKNADLLDIAKRLEIITDKDKSLHENNKLSASEACYLIVNTKKAWH
                     NTLESDTSKKDIDVLNFYFTASDFIFSEDGRILWYNDEGGALNALNTQDGSFSVITKP
                     EDPELETYPEGNNTKIVNIYDNNGVLRAVQVEEYLKPGYIVDKYILYRANENDEFRKI
                     VEFDLSTNKRYEIIGFSEDNTKLFVKTNFYDNYITIYEIDPGSESRQVVYKNRNADIV
                     DSVIAHSIGFVSTDIFH"
     gene            1262..1981
                     /locus_tag="PLI11_00285"
     CDS             1262..1981
                     /locus_tag="PLI11_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPZ51337.1"
                     /translation="MNFRKTVYKLIGDTIEHRNIGDNIILFKHNKPGTYIRKGTKIRI
                     RKNQSAFIVKDDVLYSVLRPGTYYLLPDVFPELKECFTKGLKSVVDIDLYILNQKPFA
                     SKSWSNKEAVSIKDNNLKHVQLRAFGKFSFRIEDAYKFVSEVFVKKKKYFTCNIMVHL
                     SGYVARAVGESMQEAGLGVADIINSYRSLEGTALEKLKTEGQAIGVDFMDVCIDNLSI
                     PMVVQQFFEDKKWKDIESRYK"
     gene            complement(2195..3406)
                     /locus_tag="PLI11_00290"
     CDS             complement(2195..3406)
                     /locus_tag="PLI11_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51338.1"
                     /translation="MKYPICFWNTNTLENCVPNLVETWHDLGITVAMSPSYKRDDKYK
                     MLKILDEAQDFGIKVILCDYRTHWKVLTSNGQDEYRLLAEEAHADYGHHPAVFGFFIG
                     DEPEAHQIDDALCAMRIHQEISPDLTAYLNLLPWFDWIGERMGTDALAPYLDRVKNEG
                     NAKLLSYDCYTQMWEGERGYDDYFNNLREYYLATKRLNTPYWNIVLATGHYYYRCPSK
                     IDMMWQFNTSVAHGASGVSWFYIHQTDLWDNYRNAAINPLGERTQTFYDLSEVNRMFN
                     AYCGYVITKLKIDKCFHVEKAYGGNELFTPFDNVLSVSSENKTPLIFSSFKDEEGRTY
                     YIVCCNSIKNNTLATIKVKDTVRLEKCLMGGRFVPIRPHTDPIGHREGQSAHSVNIWL
                     APGQLVLLREA"
     gene            complement(3448..4164)
                     /locus_tag="PLI11_00295"
     CDS             complement(3448..4164)
                     /locus_tag="PLI11_00295"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPZ51339.1"
                     /translation="MNNKRDFLAEIIKDSDNIVFFGGAGVSTKSGIPDFRSADGLFSS
                     KYKYPPEVMLSHTFFMNHTEDFYHFYREKMIYRDARPNKAHIKLAELEQEGKLKAVVT
                     QNIDGLHQLAGSKNVLELHGSVHRNYCMKCRKRYSLDYIHDTQGIPRCECKGIIKPDV
                     VLYEESLDNHVLFKSISYISNADVLIVGGTSLNVYPAAGLVDYYNGDKLVLINKSETP
                     MDYKANLIIHDSIDEVLGDI"
     gene            complement(4157..4786)
                     /locus_tag="PLI11_00300"
     CDS             complement(4157..4786)
                     /locus_tag="PLI11_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPZ51340.1"
                     /translation="MEKQVLLQAEERTELKTRASKRLRKEGFVPAIIYGKNIDSVPIK
                     VRSDELRRVVKEHGRNAIITIQLNGDKSYNAIIKNIFFEKMKNDLQHVDFQHVSMDEE
                     IKAVAPIRIVGREALESRRLVLIRQIDEVKIVGLPQDIPEHVEIDVSDMEAGDTVTIG
                     DIKYPDGITPDEDTDGVVLTVSEPKEEAESTDDEVKEPELVDKEDGEDE"
     gene            complement(4895..5482)
                     /locus_tag="PLI11_00305"
     CDS             complement(4895..5482)
                     /locus_tag="PLI11_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51341.1"
                     /translation="MRRAFCLCLGFLCIFILATPKVVEFKNYYTGDAAQLYSPPPDCV
                     LYIIPDISNTGTITERIGGRNETTISIVIGEDNKISWNSNFPVRAIIVQSTKGYYLYE
                     YNQATLNGEFVPSDVDDLKYVAVVYGEPESTLGETLAPTGSSQTPGTSVVPSPTDDIP
                     ITADSRIGFIYTGVILLLGATICAAVLGFVKNRTK"
     gene            complement(5581..5775)
                     /locus_tag="PLI11_00310"
     CDS             complement(5581..5775)
                     /locus_tag="PLI11_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51342.1"
                     /translation="MFFTALIPVCTVPTATPTPTSGESNEMLVFAILLVVFFLLGFAL
                     GYAFKYKTGCRKKRKYNYMT"
     gene            complement(5832..6998)
                     /locus_tag="PLI11_00315"
     CDS             complement(5832..6998)
                     /locus_tag="PLI11_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HPZ51343.1"
                     /translation="MSNEKKILNHLIDKYENSKSFTNTNKVNQRFRVKITRLFPKYAD
                     QSDYETFSSINESISFLERKGYVKAEIKRANVCESVCLNTDMLDEVYEYLGRIPKKDI
                     NNELLLLLEKYKNKNEVLANFCTEQIERIKDNRNVQFFSNDIGEFENVLKAVYALYMV
                     EKETYLRDFSIQVFRDSKTFEQISSKVTSLLFDYGDFPERESILESLNIVKNPTYVNF
                     KGQGVVTINGQTIDFSKLDGDIAISSPLIDSIEDVKVTGKGVMTIENLTSFNTFDDKS
                     FFVIYLGGFHNTTRRNFIKLIHKNNPDTSFYHFGDIDAGGFYILSHLRRMTNILFIPY
                     KMDKHTLIKYAEFTKKLTQNDKERLKRINEQDFKETIEYMLENNCKLEQEAVKD"
     gene            complement(6991..10215)
                     /locus_tag="PLI11_00320"
     CDS             complement(6991..10215)
                     /locus_tag="PLI11_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPZ51344.1"
                     /translation="MKMRKMKKTLCRIRLINWHYFVNETVSVKGSCLITGENTSGKST
                     ILDALQFVLTTSQRNFNKAANEKSNRALKGYVRCKTGDEGSIYLRKGNVISYVALEFF
                     EEKTSKYFVLGAKIESPDEDSNLDIKWFREECRLENLSFINQGSPSTDEEFRRGDKKV
                     NLIRQAKEARARFAQRLGNLDDRFFDIIPKSLAFKPIDRVKDFITNFILPERNIEVDA
                     LRTQIATLDELGKLMKTTKTKIAMLEEILKKYEDITAKEKEIKINNFMINKAQLENYC
                     EDVSKLERDFEINTQKIKIEEEKKSNLEEEIERARSRKTSLEVALGQNETTRLINETE
                     QTIRMLEKDIESEKKRLANLKNALEKLYKALYALANEGVVILSKEQLDGFIKPSVIED
                     DKIHQLTILQEELTKMLEDSRKKRYELQQQKDTLEKKRIDLQKEIEKLENKKLTFPEN
                     TERLMAEIEKEFTHLGIDAEVRVFSDLLEITDHSWQNAVEGYLNTQRFYIIVEPKYYQ
                     TALHVYYRVRNKVHTVGLVNTQKLDTNAEVDVDSLAYVVESKNRYARAYANYLLNRVK
                     RCKNVDELKNHTLAITKECMLYTNYAVRKIDPKIYETPYIGAHAIEVQLRSKKGELDK
                     VLKDLDQCKTELQKTEDLISKLEAVSFETIKENFNAPSMLALHEENLKKEKAELEKAK
                     SDPSYMEIQMQMIEAEKEINRLKEKVNNSISLISDIQFNIKFANQTILDTKLKIKAQE
                     EILERMYEEDATSANEGLAKYNEQRKTKSNETIVQNFSPMKVGLENAKNRFVKELMGL
                     QTEYKNAHQIDLGIGVEQINEYINEHHKLRSSEIVKYEEELREAKEKCELEFRENFLA
                     RLRENIENARIEFKNLNYALKDIYYGEDSYRFVLEPSKEKKHIYDMIMSDHNIAGHNL
                     FSLSFEEQYKEEMEDLFTKLIVSDDMGDKVVKEYTDYRNYLNYDIEVVKKDGSKQKFS
                     KIYGEKSGGETQTPYYVAIAASFAQLYSHGDTVRLLLFDEAFDKMDDNRISSMMDFLN
                     SQDFQIILATPTNKMDVIGEKVKSILLVYREGHNSIIQEFKENE"
     gene            complement(10175..10807)
                     /locus_tag="PLI11_00325"
     CDS             complement(10175..10807)
                     /locus_tag="PLI11_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HPZ51345.1"
                     /translation="MDIFSESTAQKEKFRTCANKLLNSCFILKRKEDTRNDYIYLLQN
                     KDYFIQYFDLLGYQLDINETYGVVSLISTSGTGRIRLKKNESILLLILRLLYIEKAKE
                     LRLVDDVVARIEEIHEKYDMLNINSKQKLDRTSLKSVFSTFKRYNLIQTMDRDITAYD
                     ARIIIYPSILFAITHDSLDAMYNAVMDKLDSYNMRGEDEDEEDEEDIMQD"
     gene            complement(10813..12141)
                     /locus_tag="PLI11_00330"
     CDS             complement(10813..12141)
                     /locus_tag="PLI11_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HPZ51346.1"
                     /translation="MIIYDTYRSELSFGVERDVLVLKLEQYFDDQLEFEQVFEEDEET
                     AVNSKEKANLVLRKLKDCGWIEYETASDYKTMVNLKDYASTMIESFIKITRQDELEYQ
                     SLISQIHATLLNQDAYVKPYEYIIKRVHENTEELIVGLKKLNTNIKQYIEKLTNDKPV
                     NEVLDDLFKYQNEIGSKAFHRIKTSDHISRFRISIRENLSRILNDENIFRKAVKGYME
                     IEMGDKKDELAAEQNLRQMVLGVLRAFENYDDIVKEIEEKHKKYWQSSVARAKFLISN
                     TNNAEGKIITILKYMAQKFNMDEETNMYDEMDEEFHELFRMFPQSWLGHESVYTERIT
                     REVSPPSKLAAGIDFSDEERQEIKNRLVEKQKNKLSRKNINEYVAKLLEDKQSVVASS
                     LPLETKRDFIKLILIYLYGKGPAAKYRSVDMDKRVVVNKFEFKDFLIERR"
     gene            complement(12282..12764)
                     /locus_tag="PLI11_00335"
     CDS             complement(12282..12764)
                     /locus_tag="PLI11_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPZ51347.1"
                     /translation="MDMLDYAINMELEGKEYYEKQSELNKNNRLSVVFRFLAEQERLH
                     AEILMRRKENKKAVPEDNEKLEINSLFSDLDDFKAEVSVIGKQLDVYRFALEIEEKSI
                     KVYEEMYEKADNDEDKKLLAFLIDQEKAHYEMFNQLETLVRKPEEWIESPEFGLREDY
                     "
     gene            12891..>13206
                     /locus_tag="PLI11_00340"
     CDS             12891..>13206
                     /locus_tag="PLI11_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51348.1"
                     /translation="MKRLIASMMLFVVSLSIFVPIRYVFKLDERVDMESKCYSIPNVQ
                     YIGYIESGLKYKFTPFCDVVFDKKYEKQLREKAKVFAEDLELLCGYYIQAAPGTHPGK
                     GNI"
BASE COUNT         3490 a         2712 c         2371 g         4633 t
ORIGIN      
        1 ggtgaaatat gtcggtggag acaaagccta tgctgtgtgc aattacgctg tccactatgt
       61 ctgcattcct gtttttatac acaacctgcc ttgactccga accggggtca atttcataga
      121 tggttatgta gttgtcataa aagtttgttt taacaaaaag cttagtgttg tcttccgaga
      181 agccgataat ctcatatcgt ttgttcgtgc tcaggtcaaa ctcaacaatt tttctaaact
      241 catcattttc attagccctg tagagaatat atttgtccac aatgtagccg ggtttaagat
      301 actcttcaac ctggactgct cttaaaacgc cattgttgtc atatatgttt acaatcttcg
      361 tattgttgcc ttccggatag gtttccagtt ctggatcctc cggtttggtt ataactgaaa
      421 aggaaccgtc ctgagtgttt aatgcattca atgcaccgcc ttcgtcattg taccaaagta
      481 tcctgccgtc ttcggagaat atgaaatcag aagcagtgaa atagaaattt aatacatcta
      541 tatctttctt gcttgtgtcg ctttcaagtg tgttatgcca tgcttttttg gtgttgacta
      601 taagatagca ggcttcgctg gcagacaatt tgttgttttc atgcaacgac ttgtccttgt
      661 cggttattat ctcaagtctt ttggcaatat ccaacaagtc tgcatttttg ggaagtttta
      721 ctcctgttat cttgaagaga atgtccagcg catcgttcct tcttaatact tcttttccaa
      781 tactttttac atatccatgt tttaaagcta ccttgcttgc atcttctccg tgttcaaatg
      841 ttagataggg tattgctgta tccaaaaaaa cagacatgtc cacctgggct gtgggagaaa
      901 gcttgcccaa ctgggtgtcc gatatttttt ccatggcatt tatatattgt atgttttcag
      961 cttcagcagg tttcctggga aataatatgg cgactgtaag cagtatcagc atcgatattg
     1021 tagttattat caagaggccc ttcttattct ccataattca aactaactcc ttaagtaaaa
     1081 atatatcaaa caataatata ttgagaaatg tcattcgtac atagaggtta tgtttaataa
     1141 gtattatatc acatttattt aattttgtat tattttattg attttgtagc gttttatcaa
     1201 gatattgaaa aattttacga attatatata atgaaagaaa aggttcgggg agggcggaaa
     1261 cttgaacttt agaaagaccg tgtacaaact aataggagat actatagagc atagaaatat
     1321 tggagacaac attatcttgt ttaagcacaa caaacccgga acatatatta ggaaaggaac
     1381 caaaattcgt atccggaaaa atcaaagtgc tttcatagtc aaggatgatg tattatattc
     1441 tgtactgaga ccaggtacat attatcttct gccggatgtt ttccctgaat tgaaagagtg
     1501 ttttaccaaa gggctcaaat cggttgtaga tatagacctc tatatactga atcaaaaacc
     1561 ttttgcaagc aagtcctggt ccaacaagga ggcagtttca ataaaggaca ataatttaaa
     1621 gcatgtacaa ttaagagcat ttggcaagtt ctcatttaga atagaggacg catacaagtt
     1681 tgtgtctgaa gtttttgtaa aaaagaaaaa atactttacc tgcaacataa tggtgcattt
     1741 aagcggctat gtggcaaggg cagtgggaga gagcatgcag gaagcaggac tgggtgtggc
     1801 tgatataata aactcataca gatctctgga aggcactgcc ctggaaaagc tcaaaacgga
     1861 aggacaagct attggagtag actttatgga cgtttgcata gataatctgt ccataccaat
     1921 ggtggtccaa cagtttttcg aggacaagaa gtggaaggat attgagtcaa ggtataaatg
     1981 aatggaggca aaaagtgcct ccattggttc aaccttttca aggaaggaaa gtgtgtcact
     2041 ggcatatttg ccatcagtat ttaataagta ctgatcaaaa cctccgttgt tgacttccat
     2101 aatatatgta tctgccatga acaagttttt ctctggctcg ctcaggcaat actggtgttt
     2161 ttttgagtaa cttttttatt ttctccggca ttaattacgc ctcccttaac aaaacaagct
     2221 gacctggtgc cagccagata ttgactgagt gagcgctctg tccttcccta tggcctattg
     2281 gatccgtatg aggtctaatc ggtacaaacc ttcctcccat caaacatttc tccagtctaa
     2341 cagtatcttt gacttttatg gttgctagag tgttgttttt aatagagttg cagcaaacaa
     2401 tatagtaggt tctgccctct tcatccttga agctggagaa aatcagtggg gttttgttct
     2461 cagaagatac cgacaataca ttgtcaaaag gtgtgaacag ctcgttgccg ccataggcct
     2521 tttccacatg aaagcattta tcaattttga gctttgtaat tacatatccg cagtatgcgt
     2581 tgaacattct atttacttct gagagatcat aaaaggtttg tgttctttca ccaaggggat
     2641 ttattgctgc attacggtaa ttatcccaca ggtccgtctg gtgaatatag aaccaggata
     2701 caccagaagc accatgagca actgacgtat tgaactgcca catcatatca atctttgaag
     2761 ggcaacgata gtaataatgg ccggtggcaa gaactatgtt ccaataaggg gtattgagtc
     2821 tttttgttgc caggtagtac tccctcaagt tgttgaaata atcatcgtaa cctctttcgc
     2881 cttcccacat ttgggtgtag cagtcatagg atagaagttt tgcattgcct tcattcttaa
     2941 ctctgtcaag atatggagcc agtgcgtccg ttcccatcct ttcgcctatc cagtcaaacc
     3001 agggaagaag atttagatac gctgtcaaat ccggggaaat ttcctggtga atgcgcattg
     3061 cacataatgc gtcatcaatc tgatgtgcct caggctcgtc tccgataaaa aaaccaaaaa
     3121 cggcagggtg atgaccgtaa tccgcatgag cttcttctgc aagaagtctg tactcatcct
     3181 gtccattgct tgtaaggacc ttccaatggg tcctatagtc gcacaaaata accttgatgc
     3241 caaagtcctg agcctcatca agaattttca gcattttata cttgtcgtct cttttgtagc
     3301 ttggggacat ggcaacggtt atgcctaaat catgccatgt ttcaacaaga tttggcacac
     3361 agttttccag agtgttggtg ttccagaaac aaatagggta tttcataata atacctcccg
     3421 tttttttaca ttgtagcaaa cgggaggtca aatgtcacct aaaacttcat caatactgtc
     3481 gtgtatgata aggtttgctt tataatccat tggagtttct gatttgttta taagtacgag
     3541 tttgtcacca ttataataat ccaccaaacc ggcagcagga tatacattca gagaggttcc
     3601 gcctacaata agtacgtcgg cgttgctaat ataactgatt gatttgaaca aaacatgatt
     3661 atcaaggctt tcctcataaa gaaccacatc cggctttatt atgcccttgc attcgcatct
     3721 tggaatgcct tgtgtatcgt gaatataatc aagcgagtat ctctttctac atttcatgca
     3781 gtaattccta tgaacggaac cgtgcagctc aagcacgttt ttgcttcctg ccagctggtg
     3841 aagaccgtct atgttttgcg taactacagc tttcagcttt ccttcctgct caagctctgc
     3901 cagctttata tgagctttgt tcggtctggc gtcgcgataa atcatttttt ctctgtaaaa
     3961 gtggtaaaag tcttctgtat gattcatgaa aaaggtatgc gacaacatta cttcaggtgg
     4021 atatttgtat tttgaagaaa acaaaccatc ggcacttctg aagtctggta ttccactttt
     4081 agtggaaaca ccagcacctc cgaagaaaac aatattatcg ctatctttaa tgatttcggc
     4141 aagaaaatcc cttttgttat tcatcttctc catcttcttt gtcaaccaat tcgggttcct
     4201 tgacctcatc gtctgtgctt tcagcttctt ccttaggttc gctgacagta agaacgacac
     4261 cgtcagtatc ttcgtcagga gtaattccat ccggatactt tatatccccg atggtgacag
     4321 tatctcctgc ttccatatca gatacatcaa tttccacatg ctctggaata tcttgtggaa
     4381 gacctacgat tttaacttca tcaatttgtc taatcaatac aagtcttctt gattcaagtg
     4441 cttctcttcc aactattctg attggggcaa cggctttgat ttcctcgtcc atggaaacat
     4501 gttgaaagtc tacatgttga agatcgttct tcattttttc gaagaagata ttcttgataa
     4561 ttgcattgta ggacttgtct ccattaagtt gaattgtaat aattgcgttt ctgccatgct
     4621 ctttaacaac tcttctaagt tcgtcactcc ttacctttat gggtactgag tcaatattct
     4681 ttccgtatat gatcgcaggc acaaaacctt cttttctcaa tctcttgctt gctctagttt
     4741 tcaattcagt acgctcttct gcttgaagca gtacttgttt ttccatagta tttcctccct
     4801 aataaaatta aatttgtaag gctagacatc tttatttaaa cacaattcat ccatgcatgt
     4861 caagaaatac ataattcctt gtgttaggaa ggtgctattt cgtcctgttt ttcacaaatc
     4921 ctagaaccgc agcgcaaatc gttgcaccga gcaaaagaat aacccctgtg taaataaaac
     4981 caattctgct atctgcagta atcggtatgt cgtcagtcgg cgaaggaaca accgatgtgc
     5041 ctggcgtttg tgaacttcca gttggtgcca gggtttcacc cagagtgctc tcgggttctc
     5101 cgtacacaac ggcaacatat ttcagatcat cgacgtcaga aggaacaaac tctccgttca
     5161 gggttgcctg gttgtattcg tacagatagt acccttttgt actctggact attattgccc
     5221 ggacaggaaa gttgctgttc catgatattt tattatcttc acctataact atactgatgg
     5281 tggtttcgtt ccttcctcca attctttctg ttatggtgcc tgtattgctg atatccggta
     5341 ttatgtaaag gacacaatct ggtggtggag aatagagctg tgctgcatcg ccggtataat
     5401 agtttttaaa ctccacaacc tttggtgttg ccagtatgaa tatgcatagg aatcccaagc
     5461 acaggcaaaa tgctcttctc atctgcgtca cctgtttaat atactctcac cacagtatat
     5521 gaattgaaaa tcatgctcgt gattggacaa tatgaagtcc cgcattaagc gggactgaat
     5581 ctatgtcata tagttgtatt tcctcttctt tctacaaccc gttttgtatt tgaatgcata
     5641 tcccagcgca aagccaagga ggaaaaacac tacaagcaat attgcaaaaa caagcatctc
     5701 atttgactca ccactggttg gagtgggagt tgcggtagga accgtgcaaa cgggaatgag
     5761 ggctgtaaaa aacaaattaa caccttcttt caaccgtttt agtatataaa tatgtaaaaa
     5821 aagattaact gttaatcttt cactgcctcc tgctcaagtt tgcaattgtt ttcaagcatg
     5881 tattctattg tctccttaaa atcctgctca ttaatccttt tgagtctctc tttatcgttt
     5941 tgagtcaact tctttgtaaa ctccgcatat tttatgagcg tgtgcttgtc cattttatac
     6001 ggtatgaaaa gtatgtttgt catgcgcctg agatgggaga ggatataaaa gccgcctgcg
     6061 tctatatctc caaagtgata gaaggatgta tctggattgt ttttgtgtat cagttttata
     6121 aagttgcgcc ttgtagtgtt gtgaaagcct ccaagataga tgacaaagaa agacttgtca
     6181 tcaaaggtat tgaaacttgt taagttttca atagtcatta cgccttttcc ggttaccttc
     6241 acatcctcta tggaatctat gaggggtgag gagatggcaa tatctccgtc aagctttgaa
     6301 aaatcaattg tctgcccgtt tattgtaaca actccttgtc ctttgaagtt aacatatgta
     6361 gggttcttta caatattcaa gctctccaga atactttccc tttctggaaa atccccgtaa
     6421 tcaaaaagca aactggtgac ttttgaagat atttgctcaa aggtttttga gtctcggaac
     6481 acctgaatgg aaaagtccct taaatacgtt tccttttcca ccatatagag ggcatataca
     6541 gctttcaaga cattttcaaa ctccccaata tcatttgaga aaaactgcac attcctgttg
     6601 tctttgattc tttcgatttg ctcggtacag aaatttgcaa gcacttcatt cttgttcttg
     6661 tacttttcaa gaagcaaaag aagctcatta tttatatcct tttttggtat ccttcccaaa
     6721 tactcataaa cctcatcaag catgtctgtg ttcagacaga ccgattcaca aacattagca
     6781 cgctttattt ctgctttgac ataccctttt ctttcaagaa aagatatgct ttcgttgata
     6841 ctgctgaagg tttcatagtc agactgatcc gcatattttg gaaacagccg ggttattttg
     6901 acacgaaagc gctggtttac cttgttggta ttggtaaagc ttttgctgtt ttcatacttg
     6961 tcaattaaat gattcaggat tttcttctca ttactcattt tctttaaact cctgtatgat
     7021 gctgttgtgt ccttctctgt aaacaagcag aatgctctta accttttccc cgatgacatc
     7081 catcttgttg gttggtgtag ccaatattat ctggaagtcc tgactgttta aaaagtccat
     7141 cattgaactt attctattgt catccatctt gtcaaaagct tcgtcaaaca gcaggagtcg
     7201 gacagtgtcg ccatgactgt acagctgggc aaaggatgca gcaatggcaa cgtaatatgg
     7261 ggtctgagtt tcgccgccac ttttctcccc atatatcttt gaaaactttt gcttgcttcc
     7321 gtctttcttt accacttcta tgtcataatt cagataattt ctgtaatccg tatactcttt
     7381 aacgactttg tctcccatgt catcggatac aatcagtttt gtaaaaaggt cttccatttc
     7441 ttccttgtat tgctcctcaa aactcaaaga aaacagatta tgtcctgcaa tattgtgatc
     7501 agacattatc atatcgtata tatgtttctt ctccttgctc ggttcaagaa caaatctata
     7561 gctgtcttct ccatagtaaa tatccttaag agcatagttg agattcttga attctattct
     7621 tgcattttca atattctctc gcaggcgggc aaggaagttt tcccgaaatt ccagctcgca
     7681 cttttctttt gcctctctaa gttcctcctc atatttcact atttcagatg atctcagctt
     7741 gtgatgctcg tttatatatt catttatctg ctcaacacca attccaagat caatctgatg
     7801 agcatttttg tattccgtct gcagtcccat caactcttta acaaaacgat tctttgcatt
     7861 ttcaagccca actttcattg gcgagaagtt ttgcacaatt gtttcattgc tttttgtttt
     7921 tctctgctca ttgtattttg caagcccttc atttgcagat gttgcatcct cttcatacat
     7981 tcgctcaagg atttcttcct gcgctttgat ttttagtttg gtgtccaaaa tggtttgatt
     8041 tgcaaactta atattaaact gaatatcact aatcaaacta atgctattgt ttaccttttc
     8101 ttttagtcta tttatttctt tttctgcttc aatcatctgc atctggattt ccatgtatga
     8161 aggatcactt tttgcttttt caagctctgc tttttctttt ttgagatttt cctcatggag
     8221 tgcaagcatg gaaggagcat taaaattctc ttttatagtt tcaaaactta cagcttccaa
     8281 cttggatatt agatcttctg tcttttgcag ttcggttttg cattgatcca aatcctttaa
     8341 taccttgtcc agctctcctt ttttactcct tagctgcact tcaatagcgt gtgcacctat
     8401 atatggagtt tcatatatct tcggatctat cttcctgact gcataatttg tgtacagcat
     8461 gcattccttt gttattgcca gagtgtgatt cttaagttca tccacattct tgcagcgctt
     8521 taccctgttt aacagataat tggcatacgc tctggcgtat ctatttttgc tttctaccac
     8581 atatgcaagg gaatcaacat ccacttcagc attggtatcc agcttttgtg tattaacaag
     8641 accaacagtg tgcaccttgt tccttacccg gtaatataca tgcagggctg tttggtagta
     8701 ttttggctcc acaatgatgt aaaaacgctg ggtgtttagg tagccctcta ctgcattctg
     8761 ccaggagtgg tccgttatct ccagcaaatc agaaaagact ctgacctctg catcaattcc
     8821 aagatgagta aactcttttt cgatttccgc cataagtctt tcggtgtttt caggaaatgt
     8881 aagctttttg ttttccagct tttcgatctc cttttgcaaa tcaatccttt ttttctcaag
     8941 agtatctttc tgttgctgca gttcatatct tttctttctg ctgtcctcaa gcattttagt
     9001 taactcttct tgcagtattg tcagttgatg aattttatcg tcttcaatta cacttggttt
     9061 aataaatcca tcaagctgct cttttgagag aataacgaca ccttcattgg ccagggcata
     9121 cagagccttg tacagctttt caagagcatt cttaagattt gcaagccttt tcttttctga
     9181 ttcaatatcc ttttcaagca ttcttatggt ttgttcggtt tcattaataa gtcttgtagt
     9241 ttcattttgt ccaagagcaa cttccaagct tgtttttcta gatcgtgctc tttcaatttc
     9301 ttcctcaaga ttgcttttct tctcttcctc gattttgatt ttctgggtat ttatctcaaa
     9361 atcccgctca agctttgaaa cgtcttcaca atagttttca agttgagctt tgtttatcat
     9421 aaaattattt atcttgattt ctttttcttt tgccgtaata tcctcgtact ttttcaatat
     9481 ttcctcaagc atggcaattt tcgtctttgt tgttttcatc agcttgccaa gttcatccaa
     9541 agtggctatc tgggttctta atgcatccac ctctatgttt ctttcaggta gtatgaaatt
     9601 ggttatgaag tccttgactc tgtctatggg tttgaatgcc agggattttg ggatgatgtc
     9661 aaaaaacctg tcatccaggt ttccaagcct ttgggcaaat cttgctcttg cttcttttgc
     9721 ctgccttatt aaattgacct ttttgtcgcc tctcctaaat tcttcatccg ttgaaggact
     9781 gccttggttt atgaaagaca aattttcaag tctgcactct tccctaaacc attttatatc
     9841 gagattgctg tcttcatcag gggattcaat ttttgcaccc agcacaaagt acttgcttgt
     9901 cttttcttca aagaactcca gagcaacgta ggatattaca ttaccttttc ttagatagat
     9961 ggatccttcg tctccggttt tgcatcttac ataccctttc aatgccctgt tgcttttttc
    10021 atttgctgcc ttgttgaagt ttctctgact tgtagtcagg acaaattgaa gggcatccag
    10081 tatggtagat tttcctgaag tgttctctcc ggttatgagg caggatcctt ttaccgatac
    10141 tgtttcattc acaaagtaat gccagtttat tagtctaatc ctgcataatg tcttcttcat
    10201 cttcctcatc ttcatcttca cccctcatgt tgtatgaatc caatttgtcc attaccgcat
    10261 tgtacattgc atcaaggctg tcatgggtaa ttgcaaacag tatcgatgga tatatgatga
    10321 tccttgcatc gtatgcggtt atgtccctgt ccatcgtttg aataaggttg tatctcttaa
    10381 aagtggaaaa aacgcttttt aatgatgttc tgtcaagttt ctgctttgaa ttaatattca
    10441 gcatgtcata tttttcatga atctcttcta ttcttgccac cacatcgtca acaaggcgca
    10501 gttcctttgc cttttcgata tatagaagtc tcaaaatgag aaggagtatg ctctcgttct
    10561 ttttgagtct gattctgcct gtgccgcttg tacttatcag ggaaacaacg ccatatgttt
    10621 cgtttatgtc cagctgatat cccagcaaat caaagtattg tataaaatag tccttgttct
    10681 gcagaaggta tatatagtcg ttcctggtat cttctttgcg tttgagtata aagcaactgt
    10741 ttagaagctt gttggcgcaa gttctgaatt tttctttttg tgcagtgctt tcgctgaata
    10801 tatccacctg atttatctcc tttcaataag aaaatcttta aattcaaatt tgttgacaac
    10861 aactcgtttg tccatgtcta cagatctgta ttttgctgca ggtcctttcc cgtaaaggta
    10921 aataagaatg agcttgataa aatccctttt tgtctcaaga ggaaggcttg aggcgactac
    10981 agactgcttg tcttcaagaa gctttgccac atattcattg atgttctttc ttgacagctt
    11041 gttcttttgt ttttccacga gtctgttttt tatctcttgt ctttcttcat cagaaaagtc
    11101 aataccggca gccagcttgg aaggtggtga cacttccctt gtaattcttt cagtgtacac
    11161 tgattcgtga ccaagccagc tttggggaaa catcctgaac aactcatgaa actcttcatc
    11221 catttcatcg tacatgttgg tttcttcatc catgttaaac ttttgtgcca tgtacttgag
    11281 gatggtaatt atctttccct ctgcattgtt tgtgttggat atcaggaatt ttgccctggc
    11341 caccgaactc tgccagtatt ttttatgttt ttcctctatc tctttaacaa tgtcatcgta
    11401 gttttcaaaa gctctaagta caccaagaac catctgtctt agattctgct cagcagccaa
    11461 ctcatccttt ttatctccca tttcaatttc catgtagcct ttaactgctt ttcggaagat
    11521 attctcatca ttcagtattc ggcttaggtt ttctcttatg gaaattctaa atcttgagat
    11581 atggtcgctg gtttttattc tgtggaaagc tttggagcct atttcgttct gatatttgaa
    11641 aagatcatca agaacttcgt taactggttt gtcattggtc agtttttcaa tgtattgttt
    11701 aatatttgtg tttagctttt tcaggccgac aataagttct tccgtattct catgaaccct
    11761 tttgataata tattcgtagg gtttaacata ggcatcctgg ttgagaagag ttgcatgaat
    11821 ctgcgatata aggctttgat actcaagttc atcctgcctt gtaattttaa taaaggattc
    11881 aatcatagtg gaggcatagt cctttagatt aaccatggtt ttgtaatcag atgcggtttc
    11941 gtattcaatc cagccgcaat ctttcagttt tctcaacacc aggtttgctt tttccttgga
    12001 gttgactgcg gtttcttcat cttcttcaaa aacttgttca aattcaagtt gatcatcaaa
    12061 atattgctcc agttttaaaa ccagaacatc tctttcgaca ccaaaagata attccgaacg
    12121 atatgtgtcg tatataatca taagacaatc cacatatatg gatttgtatt tgctgtttaa
    12181 aggtttgaaa aaactttctg gtagtatgtt aaaaagattg atacaaaatc accccgcaat
    12241 ctattatacc agacagcggg gtgttattaa acagtataaa atcagtaatc ttcccttaat
    12301 ccaaattccg gagattctat ccattcctca ggctttctta ccaaggtttc caactgattg
    12361 aacatctcgt aatgtgcttt ctcctggtca attaagaaag caagaagctt tttgtcttca
    12421 tcgttgtcag ccttttcata catctcttca tacactttta tgctcttttc ctcaatctca
    12481 agagcaaatc tgtatacgtc aagctgcttt ccgataacag acacttcagc cttgaaatca
    12541 tccaaatcag aaaacagact attaatttcc aatttctcgt tgtcttcagg aaccgccttt
    12601 ttattctctt tccttctcat gagaatttca gcatgaagcc tctcctgttc tgcaaggaat
    12661 ctaaacacaa cactaagtct gttgtttttg ttaagctccg attgcttctc atagtattcc
    12721 ttcccctcaa gttccatatt gattgcataa tccagcatat ccatgtcatc aactcccgtt
    12781 cgcttttgct gtatcttatg ctcctagtat acaatatttg ccttgagtat acaatgattt
    12841 tgtcttgtga taaaattaat gcaataaaat acgctcaggt ggttgttgat atgaaaagat
    12901 taattgcaag catgatgctt tttgttgtat ctttatcaat atttgtacca ataagatatg
    12961 tattcaaatt ggatgaacgt gttgatatgg aaagcaaatg ctattcaatt cctaatgttc
    13021 agtacatagg ctatatcgag tccggattga agtataagtt cactccattt tgtgatgtcg
    13081 tttttgacaa gaaatatgaa aagcagttga gggagaaggc aaaggtattt gcagaagatt
    13141 tggagcttct gtgcggatat tatatacagg cagcaccagg aactcatccg ggcaaaggca
    13201 atatat
//
LOCUS       DAONVY010000006        12184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_4008_length_12184_cov_3.870393, whole genome shotgun sequence.
ACCESSION   DAONVY010000006 DAONVY010000000
VERSION     DAONVY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4008_length_12184_cov_3.870393"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PLI11_00345"
     CDS             <1..1210
                     /locus_tag="PLI11_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol acetyltransferase"
                     /protein_id="HPZ51349.1"
                     /translation="WSNNFRVSITLNEEIDPDRLQKALEMTLPRFPNFSLAMKKGLFW
                     YYLEHREGCPQVSEDAANPLRRFRTNKNRFLFRVRYYERRIAVEMFHVIADGNSSMLF
                     LKTLAANYLKLGGVEIPNAHGVLDINEKPSDDEMEDSYQKYASFRVNKPRKERKAYHP
                     KGVELKYNEIKIITGQINLRKLSEKTKEYKVTITEFLCALLIKCFLDRQEREGARKKK
                     PVKICIPVNLRAFYPSKTFRNFVLYANPGVDPAYGNYTFEEIVTQVHHFMGLNINEKN
                     MNAMMSTNISSERNPFLKILPLFIKNFGMLMAYRMYGESLTTCTFSNIGKMNLPDEMM
                     PYVERFDFILNRQRNNAHAVSAVGFKDKLNITFTSKLIDSDVERDFFTSLVKMGIPVK
                     IESNKNYEGE"
     gene            1213..1920
                     /locus_tag="PLI11_00350"
     CDS             1213..1920
                     /locus_tag="PLI11_00350"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HPZ51350.1"
                     /translation="MSYCVNCGVELRESESHCPLCDTKVVNPRKHYNENAERAYPRRY
                     DIVLPAENRMMTALLATILSFLPALTCVIVDYVISEKATWSLYVAIAMGVLWSFVLPP
                     IVVRKHRDVISLLIGTISLSLGLWLIEKIVPVKGWFMSLALPIVICASFFILIIILAA
                     KYVVFGVLKMMAFFFSFTALMVVVIEIVTDMYMDDNVQLQWSLIVFVPLVLVSLVMLV
                     VERKKRVKDEMRKKFHI"
     gene            2315..3517
                     /gene="srtB"
                     /locus_tag="PLI11_00355"
     CDS             2315..3517
                     /gene="srtB"
                     /locus_tag="PLI11_00355"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HPZ51351.1"
                     /translation="MHDMNNEDVEKTLDDINKMAKIEKIHEGDSYFDKLADEVEIEIR
                     DVLKHEEVLNKQLEEGTSISKSKVFRLKEGQNLMFDFPPHFLVPDRGNLVDFNENIAV
                     KVITPKKKKRKLSAKERERRKLNLLFYKFGRSVALAVMLFALGMIGEQIYGYVADHEH
                     MIRIREQYNIGKGSLIEVTPDREIPEYPMATPPRITYPQVATLQSLLENEARNSDFVM
                     WLYGEGAEIDYYVLQANDNEFYLKRDIDKEHRSSGSLFLDYRNRPERISSSGKDNKIG
                     SNNIIYGHNMRNGNMFGKLKYYQKEEVFFKHQYLYTYTKEGVTVWKIFSAYETTTDDY
                     YIQTYFSTPEAFLKFARGLQKKSVVKTDIVLSSRDDILTLSTCHRYNYNDGRFVVHAV
                     KVGTAPIN"
     gene            3535..4551
                     /locus_tag="PLI11_00360"
     CDS             3535..4551
                     /locus_tag="PLI11_00360"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HPZ51352.1"
                     /translation="MDKPKRIEKTYNPMFEQTPISDLFFSDYLPGLDPLGVKIYVYCV
                     YLSKLNQEYTEDAIAKTLGIDKSVLMEKLIELEAIGLMSVQGGIIAVCDINQKELNRF
                     YRKITSIMPDDVEIEDLNENQARNQVVKLISDRFFGGMMPGSWYSEIELWFHQYGFSP
                     DAMYMLFQHCQTNGVMTKPYIRKVAQEWAKRNIKSVQDVEEYLLRYEKFRELVRKITN
                     KLRLGRNLTEYEEEIVAKWFYTYKYDFDIIEIALKKSTSRPKFSIRIFDAMITEWFKN
                     GLNTVDMINVYEEERRKKYAQDKGRKTKPASYRDDVPQMRNYEERQYDDEFFENLYKR
                     KETK"
     gene            4548..5561
                     /locus_tag="PLI11_00365"
     CDS             4548..5561
                     /locus_tag="PLI11_00365"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPZ51353.1"
                     /translation="MRKRIYKEVNDELEKRRFNAMLLADKRKEEIFSKVPQIAVLDDE
                     IRKLGIRLGLSAINGDYRKDEIKQKLNETIGRKRALISRCGYPADYLDNVHTCMYCKD
                     TGYIKSDSGSSRCSCFKSLLIKSLLKDSNVTRAGGTFKDYDLDLYPAEPDVERYKAKE
                     SPREIQAKNLARCKEFVRNFHTKQADNLIFVGKAGLGKTFMCNCIAIALIEKGIPVVY
                     MSAPDLFDNIASKYSQDEEEREKAIELDELLTNAELLIIDDLGTERQTGSRYAELLDI
                     LNSRELMSRKRICKTIISTNLSLKNIFSAYGERVASRIIGGFELLEFVGDDIRIKHRN
                     LSC"
     gene            5712..7412
                     /gene="rho"
                     /locus_tag="PLI11_00370"
     CDS             5712..7412
                     /gene="rho"
                     /locus_tag="PLI11_00370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPZ51354.1"
                     /translation="MEDIELHQKNIKDLRIIAKKIGVKSPTTYNKTGLIEAIKEIPDY
                     KSKIAAALENMPPEEEAEKTEAAADETAKPRRRGRPPNEKKPERAPLILARQIVNSDD
                     DTKEPEITFKKFEPAVVFRRNGAAQSADTIKEDESKKTEETEKVVTEAVEKEESKEEE
                     KSTQIVEDTQESTNEPVNGRQENNKTKYDPHSDNDVDGILDILPDNYGFLRIGSYVSG
                     SKDVYVSPVYIKRFNLRTGDRIIGKSRFQKDNEKYQALYYINSINGFPPESMSKRKSF
                     DKLTPIYPDSKIVLETDSNELSTRLIDLIAPIGKGQRGIIVSPPKAGKTVLLKKIANA
                     VSVNYPEMELIVLLIDERPEEVTDMKESVKGEVISSTFDEMPERHIKVAEMVLERAQR
                     LVEHGKDVIVLLDSLTRLSRAYNLTITPTGRSLSGGLDPGALFKPKKFFGSARNLREG
                     GSLTIVATALIETGSRLDDVIFEEFKGTGNMELHLDRKLSEKRIFPAIDINKSGTRRE
                     ELLLSESELNAIYTVRKAMANLGTADVTEMLINKLMQTKTNVEFIKMINTSFNDSYKY
                     "
     gene            7539..7736
                     /gene="rpmE"
                     /locus_tag="PLI11_00375"
     CDS             7539..7736
                     /gene="rpmE"
                     /locus_tag="PLI11_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPZ51355.1"
                     /translation="MKKNIHPNLIETVAKCACGESFSTLSTRENISVEICSKCHPFYT
                     GKAKLVDTGGRVEKFNKRYGF"
     gene            complement(7806..9590)
                     /locus_tag="PLI11_00380"
     CDS             complement(7806..9590)
                     /locus_tag="PLI11_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPZ51356.1"
                     /translation="MARKTRIELNDFDEILNDERYIIDKEAKKEKRREKREKRRLIAE
                     YDKDDLIGKAERKHKATIISLIAAGSLVIIFFITTIIISATLNKGEVYAGVYFNNYSL
                     YGMTKKEIEDYINKTYIDPFKNKKLIISYEDKEIVTTLYDVTKTPDPKKVADDAYKVA
                     RNGNIYSRLFKVMNLKSEPVKMKFTLSLDKEILEQLINNLKDELYEAKEDPSYKILPN
                     SVVFRGGKNGSEMNQLKLENDINDAIYELLETKNGTSVEKVVVEISTIKFKPLNVDEI
                     FNEVYVKPVDSTYDRISPKEILITDSTPGKKLDRDALNNIVSRINKGENVIFEELPIL
                     DVEPEITREFLESTLVNYTIATSSNKNNNNDDMTDPARMEDRATNIQKCVDALNRYIL
                     FPGEEFNFWEALGTFNESTGYVYAFDNSAAAEQKVLGGGISQVASALYLAAFTSELEI
                     VEHHNYPYTVNYGPLGFDAHVVKGSKNLIFKNTLEFPIRIVCSYEDETIKVEILGTDT
                     NSNVRIRTSSTTKAISYDTEYIDDPNLPEGTEFITKGGIMGYEVQLFKRIIMNGVELL
                     SEHIGTVVYQPRNQVITRGTGQIEDIEE"
     gene            9699..9989
                     /gene="yabP"
                     /locus_tag="PLI11_00385"
     CDS             9699..9989
                     /gene="yabP"
                     /locus_tag="PLI11_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HPZ51357.1"
                     /translation="MAEERRILANTTGHSVSMDNRERLRISGVVDVYGFDDTYVALET
                     ELGKMLVRGEELKIGKLSLEHHELVVTGFIYSLEYDDKKKGKGKGVFASMFR"
     gene            10022..10429
                     /locus_tag="PLI11_00390"
     CDS             10022..10429
                     /locus_tag="PLI11_00390"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HPZ51358.1"
                     /translation="MNEGLMIGRTLQLFLIMMAVGVVIALLFDFFRAVRKATKTAYKE
                     LTYAVHVEDVLFALLAFSVFIFAVVVFNDGEIRGYMLLGLACGILVYWALISRVINKI
                     LFWVIYILFKIVSFPVKMVARIKRMVKKRKEKN"
     gene            10563..10832
                     /locus_tag="PLI11_00395"
     CDS             10563..10832
                     /locus_tag="PLI11_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPZ51359.1"
                     /translation="MKKLFNLRNIIFIGIIVNLIVILLSQQSILDRNMKNYRDLQEQI
                     QAETEQNEKLKEEQEMIGTYEYIERKAREKLGYVKKDEIVFINEK"
     gene            10853..11332
                     /locus_tag="PLI11_00400"
     CDS             10853..11332
                     /locus_tag="PLI11_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPZ51360.1"
                     /translation="MVNVNDIVEGKVTGITAFGAFVQIQDGTTGLIHISEISTGYVSD
                     INDYLKVGQTVKAVALSVEKDGKLSLSLKRYLELEQGEKPKSIKREYKKQDRKPNKGM
                     QGKGNLPYSPPPVYEPSKENSSFEDKLSKFMKDSDEKLSALRKNTESKRGGGYVRRG"
     gene            11316..12149
                     /locus_tag="PLI11_00405"
     CDS             11316..12149
                     /locus_tag="PLI11_00405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPZ51361.1"
                     /translation="MYDADKAADILKLVSLSLVNNAGYINDKSGNPMLYKRELGAESV
                     VYTINAREVTSDLVIFNLDRLIDNFMHTPQSKKVFFYFLFVGEKSNVDEFKHVLSGKF
                     DECYEKGLLCEAIFVDSDNLSCSTISGRKPMDRKLRKALERCLDNLKNSSIDDTLKKQ
                     EKEYVQMQRELRPIAKVGFFNAAFVLILINIFVFLVDGYIYLSSGRMPLLALGIQDNE
                     LIRNGETWRLVTSMFLHADIAHLAGNMLSLFYGFIYFKACSSFSSPFCRRFMASSLPK
                     R"
BASE COUNT         3882 a         2064 c         2725 g         3513 t
ORIGIN      
        1 gtggtccaac aatttcagag tatcaattac cctgaatgag gaaattgatc ctgacaggct
       61 gcagaaagct cttgagatga cgctgcccag gtttcccaat ttctcccttg ccatgaaaaa
      121 gggacttttc tggtactacc tggagcacag ggagggctgc cctcaggtct cagaggatgc
      181 tgcaaatcct ttaagaaggt ttagaacgaa caagaaccgc tttctgttca gggtcagata
      241 ttatgaaaga cgaatagcag ttgagatgtt ccatgttatt gcggatggaa acagctccat
      301 gctgtttctt aaaactcttg ctgccaatta tctcaaattg ggcggagttg aaataccaaa
      361 tgctcatgga gttctggata taaatgagaa gccttcggat gatgaaatgg aggattcata
      421 tcaaaaatat gcttccttca gggtaaacaa gccgaggaag gaaaggaaag catatcatcc
      481 taaaggtgtc gagcttaagt acaacgagat aaagataatt acgggacaga taaatcttag
      541 aaagctttcc gaaaagacaa aagagtacaa ggttacaata acagagtttt tgtgtgcatt
      601 gcttattaaa tgctttttgg acaggcagga aagggaaggt gcaagaaaaa agaaacctgt
      661 aaagatatgc attcctgtga acttaagagc tttttatcca agcaagacat ttagaaattt
      721 tgtattgtat gcgaatcccg gggtggatcc ggcctacgga aactatacct ttgaggagat
      781 agtaacgcag gtgcatcatt tcatgggatt aaacataaat gagaagaaca tgaatgcaat
      841 gatgagtaca aacatttcaa gcgaaaggaa tcctttcttg aagattctgc ctttgtttat
      901 aaagaatttt ggaatgctta tggcctacag aatgtatgga gaatccctta ccacatgtac
      961 tttcagcaac atcggaaaga tgaaccttcc cgatgaaatg atgccttatg tggaaaggtt
     1021 tgacttcata cttaacaggc agagaaacaa tgcccatgct gtttctgcag tggggttcaa
     1081 agataagttg aacattacat ttaccagcaa gctgattgac agtgatgtgg agagggactt
     1141 ttttacctct cttgtcaaaa tgggcatacc tgtaaaaatt gaaagcaata aaaattatga
     1201 gggtgaataa acatgtcata ctgtgtcaat tgtggtgttg aattaagaga atcagaaagc
     1261 cattgtccat tatgtgacac gaaggttgtc aatcccagaa agcattacaa tgagaatgcg
     1321 gagagggctt acccaagaag atatgatatc gtcctgccgg cagaaaacag gatgatgact
     1381 gcccttcttg cgaccatttt gtcttttctt cctgctttaa cctgtgtgat tgtcgattac
     1441 gtcatcagtg aaaaggcaac ctggtccttg tatgtggcaa ttgcaatggg tgttctatgg
     1501 tcatttgtac tgccgcctat tgtggtgagg aagcacagag atgtaatatc ccttttaata
     1561 ggaacaattt cgctgagttt aggcctctgg cttatagaga aaatagtgcc ggtaaaggga
     1621 tggtttatgt ctcttgcgct gccgattgtt atctgtgcat cgtttttcat actaatcatt
     1681 atactggctg caaagtatgt ggtgtttggg gtattgaaaa tgatggcgtt tttcttcagc
     1741 tttactgctc tcatggtggt ggttattgaa attgttacag acatgtatat ggacgacaat
     1801 gttcaacttc agtggtcact gatagtgttc gtgcctcttg tccttgtatc tttggttatg
     1861 cttgtagttg aaaggaaaaa gagggtaaag gatgaaatga ggaagaagtt ccatatttag
     1921 ttgtgctcaa tagtttgatt tttcctggtt catagtgtat catgatattc agttaataag
     1981 ttggagtgat atggtgaaag ttttcagact taaggaagga cagaatatta ttttttaaac
     2041 atgatgttct acaaattttt ctcttgcaat gataagtgag cagatatatg ggtatgtgct
     2101 gaccatgaac acatgatggc gttgaaacat tggaggagga aggcacagag ctgaattatc
     2161 atgttcttca ggctcaagat gtaccggaaa gaggaaattt tcttacaaaa ccagtatctg
     2221 tatacctaca gcacgttttg gtaactttat tctgtttgat tttttgcctc gttttgtata
     2281 ttatatatat cgaatctata ttggggagat attcatgcac gatatgaata atgaagatgt
     2341 tgagaagacg ttggatgata taaataagat ggcaaagata gagaagatac acgagggaga
     2401 ttcatatttc gacaagctgg cagatgaagt cgagatagag attcgtgatg tccttaaaca
     2461 cgaggaagtt ttgaacaagc aacttgaaga gggaacttcc atcagtaaat caaaggtttt
     2521 caggctgaag gaaggacaaa acctcatgtt tgattttcct cctcattttt tagtgcctga
     2581 cagaggaaat cttgtagatt ttaatgaaaa tattgccgta aaagtaataa ctcccaagaa
     2641 aaagaaaagg aaattgtctg ccaaggaaag agagagaaga aaactgaatc tcttgtttta
     2701 caagtttggc agatccgttg cacttgcggt aatgcttttt gctcttggaa tgattggtga
     2761 gcagatttat ggatatgtgg cggaccatga acatatgatc aggataaggg agcagtataa
     2821 tataggtaag ggcagcttga tagaagttac tcctgacagg gagatccctg agtatccaat
     2881 ggcgactccc ccaagaatta catatccaca ggtggccact ttgcagtcct tgctggagaa
     2941 tgaagcaaga aacagtgatt ttgtaatgtg gctttatggt gaaggggcag agatagatta
     3001 ctatgtcctt caagccaatg acaacgagtt ttatctgaag agagacatag acaaggagca
     3061 tagatcatca ggttcgctgt tccttgacta cagaaacagg cctgaaagga tatcaagttc
     3121 aggcaaggac aataaaattg ggagtaataa tataatatat gggcacaaca tgagaaacgg
     3181 aaacatgttt ggcaagctca agtattatca gaaagaggaa gtatttttca agcaccagta
     3241 cctttacacc tatacaaaag aaggtgtcac cgtttggaaa atattttctg cttacgaaac
     3301 caccacggat gattattaca tccaaacata tttttcaacc cctgaggctt ttcttaaatt
     3361 tgccagagga ttgcagaaga aatcagtggt aaaaaccgac attgttctgt cttcaaggga
     3421 cgacatactg actctttcca cctgtcacag gtacaattac aatgacggac gtttcgttgt
     3481 gcatgcggta aaggttggaa ctgcaccaat taattaacat aaaggagaat tgttttggac
     3541 aaacctaaaa gaattgaaaa aacgtacaat cccatgttcg aacaaacgcc aataagcgat
     3601 ttgttctttt cagactactt gccgggtctt gatcctttgg gcgtcaagat atatgtttac
     3661 tgcgtttact tgtccaaact taaccaggag tatacggaag atgcgatagc aaaaacctta
     3721 ggcatagaca aaagtgtgtt gatggagaaa ttaattgaac tagaagcaat cggacttatg
     3781 agcgttcaag gaggaatcat tgccgtttgc gacataaatc aaaaggagct taacaggttt
     3841 tacaggaaaa taacttccat tatgcctgat gatgtggaga ttgaggattt aaatgaaaat
     3901 caggctagaa accaggttgt aaagctgatt agcgacaggt tttttggcgg catgatgccc
     3961 ggaagctggt attctgaaat agagctttgg tttcatcaat acggcttcag tcctgatgca
     4021 atgtatatgc tgttccagca ttgtcagaca aatggtgtca tgacaaagcc gtacataaga
     4081 aaagttgccc aggagtgggc aaagagaaac attaaatcgg tgcaggatgt ggaagaatac
     4141 ctcttgcgct atgagaagtt tagagagctt gtaaggaaga taacgaacaa gctgaggctt
     4201 ggcagaaatc ttaccgagta cgaggaagaa attgtagcca agtggttcta tacatacaag
     4261 tatgattttg atataattga gattgcttta aagaagtcaa cgagcagacc caagttttca
     4321 attcgtatat tcgatgcaat gataacagag tggttcaaga acggactgaa tacggttgac
     4381 atgataaatg tctatgagga agaacgcaga aagaaatatg ctcaggataa aggcaggaag
     4441 acaaagcctg caagctatag ggatgatgtt cctcaaatga gaaattacga ggaaagacag
     4501 tatgacgatg agttttttga aaacctgtac aaaagaaagg agactaaatg aggaagagaa
     4561 tctacaaaga ggtcaatgac gaactcgaga aaagaagatt taatgcaatg ctccttgctg
     4621 ataagagaaa agaagagata ttttccaagg ttccacagat tgcagtcctt gatgatgaaa
     4681 ttcgcaaact gggaataaga cttggtttga gtgcaataaa cggagattac agaaaagacg
     4741 aaatcaagca aaaactcaat gaaaccatag gtagaaagag agctttaatt tccagatgtg
     4801 gttatcctgc ggattatctt gacaatgttc atacatgcat gtattgtaag gatacaggat
     4861 atattaagag tgattccggt tcttcaaggt gttcttgttt caagagcctt cttataaaaa
     4921 gtcttttgaa ggattcaaat gtcacccgtg caggtggaac ttttaaagat tatgaccttg
     4981 atttgtatcc tgccgaacca gatgtggaaa ggtataaagc gaaagagtct ccgcgtgaaa
     5041 tccaggcaaa aaatcttgca agatgcaagg aatttgtaag gaactttcat acaaaacaag
     5101 cagacaatct tatatttgta gggaaagcag gtcttggaaa gacttttatg tgcaactgca
     5161 ttgcaatagc acttattgaa aagggaattc cagtggtata catgagtgct ccggatttgt
     5221 ttgacaacat agcttccaag tactcgcagg atgaggaaga acgtgaaaag gcaattgagc
     5281 tggatgaact tttgacaaat gcggaacttc tgattataga tgatttggga acggagaggc
     5341 agactggttc gagatatgcc gaactcttgg acattttaaa ttcaagggaa ctaatgtcca
     5401 ggaaaagaat ttgcaaaacc ataatttcaa ctaatctcag tctaaagaat atattctccg
     5461 cttatggtga aagggttgca tcccggataa taggaggatt cgaacttttg gagtttgtag
     5521 gtgatgatat acgtataaag catagaaacc tttcgtgttg acatttttta cattattgtg
     5581 tattatattc atgagcaatt cgctcagttc aaattggtct tcatctgtta gacttgttat
     5641 cccccctaaa ttgattatta tggtagtatg gaaaatatat ggtaaaaaat atgtaataag
     5701 gtggtgtatt gatggaagat atagaacttc atcaaaaaaa cataaaggat cttcgcatta
     5761 ttgcgaaaaa aattggtgtg aaatcaccca caacatacaa taaaacgggt ctcattgaag
     5821 ctattaaaga gattccggat tataaaagca aaatagctgc tgctttggaa aacatgcctc
     5881 ctgaagaaga agcggagaag actgaagctg cagcagatga gactgccaaa ccaagaagaa
     5941 gaggcagacc tccaaatgag aagaaaccag agagagctcc tttgatattg gctagacaga
     6001 ttgtcaacag cgatgatgat acaaaagaac cggagataac cttcaaaaag tttgagcctg
     6061 ctgtagtttt cagaagaaat ggtgctgcac aaagtgccga caccattaaa gaggatgaat
     6121 ccaagaaaac tgaagaaact gagaaggtag taaccgaagc cgtggaaaaa gaagaatcaa
     6181 aggaagaaga aaaatctaca caaatcgttg aagacacgca ggaatcaact aatgaacctg
     6241 taaacggaag acaggaaaac aacaaaacga aatatgatcc acattcagac aatgatgttg
     6301 atggtattct tgacattttg cctgacaatt acggattcct aagaatcggc agttatgtat
     6361 ccggtagcaa ggacgtatac gtttcccctg tttacatcaa aagatttaat ctgagaacag
     6421 gagacaggat tatagggaag agcagattcc agaaggataa cgaaaaatat caggctttgt
     6481 actatatcaa ttcgataaat ggatttccac ctgaatcgat gtcaaaaaga aaatcttttg
     6541 acaagcttac tcccatatat ccggattcaa aaattgtatt ggaaacagat tcgaacgaat
     6601 tgtccacacg tctaatcgac ttaatagcgc caataggcaa agggcagagg ggaataattg
     6661 tttcaccgcc caaggcggga aaaactgttc tgctcaagaa gattgccaat gcggtttctg
     6721 tcaattatcc cgagatggaa cttattgtgc tgcttataga cgaaagacct gaagaagtta
     6781 cggatatgaa agagtctgta aagggtgagg ttatttcctc tacatttgac gaaatgcctg
     6841 aaagacacat aaaagtggcg gaaatggttt tggaacgcgc tcaaaggctt gttgaacacg
     6901 gcaaggatgt aattgttctt cttgacagcc tgacaaggct atcaagggca tacaacctga
     6961 caattacacc tacgggtagg tccctgtcag gtggattgga tcctggagca ttgttcaaac
     7021 caaagaagtt ttttggttct gcgaggaacc tgcgtgaagg aggaagtctg accatagtag
     7081 ctactgcctt gattgaaacg ggcagccgtc ttgacgatgt gattttcgag gagttcaagg
     7141 gaacaggaaa catggagctg cacttggata gaaaactttc tgaaaaacgt attttccctg
     7201 ccattgatat aaacaagtca ggtacacgaa gggaagaact tctgttatca gagagtgaac
     7261 tcaacgcaat ttatacggta aggaaggcaa tggccaacct gggcacggcc gatgttaccg
     7321 agatgttgat aaacaaactt atgcaaacaa agaccaatgt tgagtttata aagatgataa
     7381 atacatcatt caatgacagt tacaagtatt aagaaattcc atttttgctg aaattattta
     7441 aatatcaagt attgcacata acattgcaac atggtataat acactatgtt gtccaaaaat
     7501 atggcatgca gcaatgagta tgaaggaagg tgacaaaaat gaaaaagaac attcatccta
     7561 atctaataga gacagttgcg aaatgtgcat gtggagaatc cttttccact ttatcaacta
     7621 gagaaaatat tagcgttgag atatgctcaa agtgccatcc tttctatacg ggtaaggcga
     7681 aactggtgga tactggtgga cgtgttgaaa aattcaacaa gagatatgga ttctaagaga
     7741 tataatcaag ctcacagcaa gtgggctttt ttatatgtca aaagtaaagg agccgaatta
     7801 ggctcctatt cttcaatatc ttcgatttgt cctgttcctc ttgtgattac ctgatttctt
     7861 ggctggtata cgacagttcc aatatgctcg cttaggagtt ccactccatt cataattatt
     7921 ctcttgaaca gctgaacctc gtagcccatt atgccgcctt ttgtgataaa ctcagtacct
     7981 tcaggcaggt ttggatcatc aatgtattcc gtatcatagc ttattgcctt tgttgtagag
     8041 cttgttctta tgcgtacatt gctgtttgta tcggttccga gtatctctac ctttatcgtt
     8101 tcatcctcat aggagcaaac aatccttatt ggaaactcca gtgtgttttt aaatatcagg
     8161 ttctttgatc cttttacaac atgtgcgtca aaaccaagag gaccatagtt tactgtatac
     8221 ggatagttgt ggtgctccac tatttcaagc tcgcttgtaa aggctgccag gtaaagtgcg
     8281 gaagcaactt gggaaatgcc accgcccaag accttttgtt ccgcagctgc ggagttgtcg
     8341 aaagcatata cgtaacctgt gctttcgttg aaggtgccca aagcttccca gaagttgaac
     8401 tcttcgccag ggaaaaggat gtagcgattc agtgcatcca cacatttttg aatattagta
     8461 gctctgtctt ccattctagc agggtcggtc atgtcgtcat tgttattatt cttgttggaa
     8521 gaggtggcga tagtgtagtt taccagagtg ctttcaagga attccctcgt tatttcaggt
     8581 tcaacatcca gtattggcaa ctcttcaaat atgacgtttt cgcccttgtt tattctgctt
     8641 actatattat taagagcatc tctgtccagt tttttaccag gtgtggaatc agttataagt
     8701 atttcttttg gtgatattct gtcataagtg gaatcaacag gtttaacata aacctcgttg
     8761 aatatttcat caacattcaa tggtttgaat ttgattgttg atatttccac aacaaccttt
     8821 tcaacactgg tgccgttttt tgtttccaat agttcgtaaa tcgcatcgtt tatgtcgttt
     8881 tcaagtttca actgattcat ttcactgccg ttttttccac ccctgaaaac tactgagttt
     8941 ggcagtatct tgtagctggg gtcttctttt gcctcataca gttcgtcttt cagattgttt
     9001 atgagctgtt ccagtatctc cttgtccaaa gaaagagtaa atttcatttt gaccggttcc
     9061 gacttcaagt tcatgacttt gaacaatcgt gaatagatgt ttccgtttct agctacctta
     9121 tatgcgtcat cagcaacctt tttagggtcc ggtgttttgg taacatcgta aagtgttgtt
     9181 acaatttcct tgtcttcgta agagattatg agctttttgt ttttaaatgg atctatatat
     9241 gttttgttaa tatagtcttc aatttctttc tttgtcattc cataaaggct gtagttgttg
     9301 aaatatacgc ctgcataaac ctctcccttg ttaagagtgg ctgagattat tatagtggtt
     9361 ataaagaaga tgataacaag actacctgct gctatcaaac taattatagt cgccttgtgc
     9421 ttgcgttctg ctttgccaat caaatcatcc ttgtcgtatt cggcgataag ccttcttttc
     9481 tccctttttt ctcttctttt ttctttcttg gcttctttat ctatgatata tcgttcatca
     9541 tttagaattt cgtcaaaatc attcagctca attcgtgttt tgcgagccat ctatatacac
     9601 ctctcgtatt attctccgca tgcaaaaaga agtataacac aaaagaataa aagaataaag
     9661 gacaaatttc aagcatatta ttggttgagg tgaggcatat ggcagaggag agaagaatcc
     9721 ttgcaaacac tacaggacac agtgtttcaa tggataacag ggaacgtctc agaatatcag
     9781 gagttgttga tgtttacggt tttgacgaca cctatgtggc cttggaaacc gagcttggaa
     9841 agatgcttgt aaggggagag gagttaaaga taggaaagct cagccttgag catcatgagc
     9901 tggtggtaac cggatttatt tacagtcttg agtatgatga taaaaagaaa ggaaagggca
     9961 agggcgtatt tgcctccatg ttcagatgaa tactttgctt ctggtgagtg caggaaacgc
    10021 tttgaatgaa ggtttgatga ttggcagaac tcttcagctg ttcttaatca tgatggctgt
    10081 tggagttgtt attgcacttc tttttgattt tttcagggcg gttcgaaaag caaccaagac
    10141 agcatacaaa gagctgactt atgcagtcca cgtagaggat gtattgtttg cacttttggc
    10201 gttttcagtt ttcatttttg ccgtggtggt atttaatgac ggtgaaataa gaggatacat
    10261 gctgcttggg cttgcttgcg gcatactggt ttactgggca ctgatatcac gtgtaattaa
    10321 caagattctg ttctgggtaa tctatatatt gtttaaaata gtgtcctttc ctgtgaaaat
    10381 ggtggcaagg ataaaaagga tggtgaaaaa aagaaaagaa aaaaattagc aggtgttgtc
    10441 aattatatcc tgttgtgata ttatgtgcat aaagttgttg attttgatta aaaaggtact
    10501 agaccttgat aataaaacaa tatataatat cggtgtacta aagaaaagat gaggtaatgg
    10561 ttatgaaaaa actgttcaat ctcagaaaca ttatatttat aggtataatt gtcaatctca
    10621 tagtgatact gcttagccag caaagcatat tggataggaa catgaaaaac tatcgtgact
    10681 tgcaggagca aatccaggct gaaacggaac aaaacgaaaa gctgaaggaa gagcaggaga
    10741 tgataggcac ctatgaatat attgaaagga aggccagaga aaagctgggc tacgtaaaga
    10801 aagacgaaat agtatttatt aatgaaaaat agcaggaggt cttatttatt ttatggtaaa
    10861 tgtcaatgac attgtagaag ggaaagtcac gggtatcaca gcatttggtg cctttgttca
    10921 aatccaggat ggaactacag gtctgataca catatctgaa atttcaacag gttacgtaag
    10981 tgacatcaat gactacttaa aagtgggaca gacagtgaaa gctgttgccc tttcagttga
    11041 gaaggacgga aagttaagtt tgtccttgaa acgttatctt gaactggaac agggagagaa
    11101 accaaaaagc ataaagaggg agtacaagaa acaggacaga aagccaaaca aaggcatgca
    11161 gggcaaagga aacttgcctt attctccgcc tcctgtgtat gagccttcca aagaaaacag
    11221 ctcctttgaa gacaagttga gtaaattcat gaaagacagc gacgaaaaac ttagcgccct
    11281 aagaaaaaac acggaaagca aaaggggcgg aggttatgta cgacgcggat aaggctgcag
    11341 acattctaaa gcttgtttcg ctgtcgcttg ttaataatgc tggctatatt aatgacaaat
    11401 cgggaaaccc gatgttatat aaaagagagt tgggtgcgga atctgttgta tatacaataa
    11461 atgcaagaga agtcacatct gatttggtca tttttaatct tgacagatta atcgacaatt
    11521 tcatgcatac accgcaatca aaaaaagttt tcttttactt tctgttcgta ggcgaaaagt
    11581 caaatgtcga cgaatttaaa catgtattgt ccggcaaatt tgacgaatgc tacgaaaagg
    11641 gtcttctgtg tgaagcaatc tttgtggatt cggacaattt gtcctgctcc acaatttccg
    11701 gcagaaagcc aatggacaga aaattaagaa aagcactgga aagatgtctg gacaatctga
    11761 aaaactccag tatagatgat actttgaaaa agcaggaaaa ggaatatgtg caaatgcaga
    11821 gggaactaag gcctattgcg aaggttggtt tttttaatgc ggcatttgtt ctgattctga
    11881 taaacatatt cgttttcctt gttgatgggt acatctacct ttcatcaggc aggatgccgc
    11941 tacttgctct gggcatacag gataatgagt taataaggaa tggagagacg tggaggcttg
    12001 ttacctccat gttcctccat gcggatattg cacatttggc aggaaacatg ctttcacttt
    12061 tctacggctt catttatttc aaagcctgct ccagtttttc cagccccttc tgcaggcgtt
    12121 tcatggcttc ttccttgcct aaaaggtaaa gaacatctat ggcgcctccc ggtgtaacct
    12181 tctg
//
LOCUS       DAONVY010000007        11696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_4239_length_11696_cov_3.385792, whole genome shotgun sequence.
ACCESSION   DAONVY010000007 DAONVY010000000
VERSION     DAONVY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_11696_cov_3.385792"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLI11_00410"
     CDS             <1..391
                     /locus_tag="PLI11_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HPZ51362.1"
                     /translation="HGGGAFSGKDPTKVDRSGAYMARYIAKNIVAAGLADKCEIQIAY
                     AIGVARPVSVRIETFGTERAEVSKLEQAVIDIFDMRPSSIINEFNLRSPLYGKLAAYG
                     HFGRTDLSLPWENTDKVDLLIEKVRNS"
     gene            436..1098
                     /locus_tag="PLI11_00415"
     CDS             436..1098
                     /locus_tag="PLI11_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51363.1"
                     /translation="MKYCEYVAKSTKRIIEPFASFLYFSLLCAEKGFEGDFWFDFNDE
                     ILAHLFGLIIKSPEFVSDGYEKLWSCQFDFDKDNYFNCLKEKYEYVDFTEKAVILSFL
                     IQKSKKDLTEDNATYGRKPKSMRETLKRISFLLKDRTKITVKDIDVFKGRAEDTYFLE
                     NVTCLDKNVQKMIENGAGFIMVLEKDLGEKKRNNLVSKLKSTTNIHFDYINNVICSKG
                     AG"
     gene            1099..2277
                     /locus_tag="PLI11_00420"
     CDS             1099..2277
                     /locus_tag="PLI11_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPZ51364.1"
                     /translation="MSTPKAELEKRILNLYDVMKDKDYDTIIIINRINQYYFTGTMQD
                     GVLVLRKDGTVMFFVRRSYDRAKLECPLDLVYPMKTYKDILDYLPNELGVVYMETQTV
                     TIAIKERLQKYFKFDAVKSIDKEILVLRAVKSDYELEFIKESGRQHNYLMTSIIPSIL
                     REGMSETDFLSELYTHMVKLGYHGISRFNMFQMEMVIGQTGFGDNSTYPTNFDGPGGM
                     KGMSPAVPIIGDRNRYLKKGDIVFVDVGYGVDGYHSDKSQVYSFKAKPDDRALEIHRA
                     CMIAQKTTADMMKEGAIPSEIYEEVMASLPDCLNENFMGMYDPVRFLGHGVGLHIDEL
                     PIIAKGFNMPLKQNMVVSLEPKCAVKNIGTVGVEETYVIGRDGAECITGGACDIIVVD
                     "
     gene            complement(2274..4619)
                     /locus_tag="PLI11_00425"
     CDS             complement(2274..4619)
                     /locus_tag="PLI11_00425"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPZ51365.1"
                     /translation="MDNNNFNFTNCKWLWVNAARDEKYENIYTQYMDSFTITEEPQKI
                     VLHISADSEYVAWVNGKLAGYGQYADFPEYKVYDSLDITSLVNDIGEKNTLCILTYHQ
                     GRSTSVYKRGLPGVIYGVEQDNSTVLYSSGNALCRISPDYMSGTLPLTTPQLSFVFEF
                     DFNGYDNWNQEGYEYAMVGDMAWGKAYAYENTQKLFPRPIKKLELGDRIEPKIVAQGV
                     YKYSAHAEPETTAAKMQFSYLSYRDAHVLYGDAPCTTLPKENGLIFKNNSGDDDGIYI
                     VLDLHREESGVFEIDITAPEGTVINIGYGEHLDDLRVRSYVGTRNFSGKLTASGKRDQ
                     FTHYIKRFGCRYIQLYVEAKEFTLYYAGLRPTNYPIEYKGVFACSDSLHNKIYEISLR
                     TLLLCMHEHYEDCSWREQALYAMDSRNQMLCGYYAFGEYDFARESIRLLGLSQRDDGF
                     LELCAPAEIPITIPCFTLMWIVQLYEHILFTGDLDFAQEMWKHVEQIIRSVWIHSRGS
                     DLVPPYYNDKYWNFYEWSDGLAGDRSLNNMRDKDNPLNFDAPLNAFYYLALRSAVQIA
                     KNLADYTKDANYHDLEAWYDMMAAGVLNNFDKTFWDEEKEVYATYLINGEKHHYSELT
                     NALALYAGLVPESKKEKVAHMLAHGKDLVPITISHSIFKYEALLSVSDEYGKYVFDEI
                     TDKWGYMLYNNATTFWETINGAWDFSNAGSLCHGWSAIPVIFYYKYVLGISPLTVGFT
                     DYKFSPIPTPLYAAEGKIPSGKMDYHVSITPNGFKINKIKH"
     gene            complement(4688..5389)
                     /locus_tag="PLI11_00430"
     CDS             complement(4688..5389)
                     /locus_tag="PLI11_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPZ51366.1"
                     /translation="MNKTVIGVTSNRQPTVWEGSIEGSYCHSIMQSGGLPLLIPPVEN
                     EEHFDWLMKCCDGFLFTGGIDIHPKYFGEDILNDTVEIDEIRDAFELKLIKRAIDEDK
                     PLLAICRGVQVLNVALEGTLYQDLPTQLDINHRDLRPLLEGGHEVTILKDSPLYKIIK
                     KNIIMVNTRHHQSIKDLGKGLSVMAVSNDDVIEAVYMPEKRYVVGVQWHPEMLAHKYK
                     THKVIFDDFIKHCQC"
     gene            5504..6157
                     /locus_tag="PLI11_00435"
                     /pseudo
     CDS             5504..6157
                     /locus_tag="PLI11_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
     gene            complement(6154..6870)
                     /locus_tag="PLI11_00440"
     CDS             complement(6154..6870)
                     /locus_tag="PLI11_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPZ51367.1"
                     /translation="MKEFLTKPIAHRGLHNKKYPENSLSAFKNAIDYGFPIEIDIQLA
                     SDNNIYVFHDANLKRMTGFDKPINESSKEEIRRLRLADTNEHIPTLEEVFVLVDGQVP
                     LLIEIKKCGRPKEIAKNLSEMLETYKGKVAVQSFYPDYLYHFKRYSPKIKRGQLATAS
                     FPDDIPQIQRFVSRNMFGNPIVRPSFITYDFKCLPRNVVSFYKKFGLPVLGYTIRSKE
                     EETQARKYCNNIIFENYVPD"
     gene            complement(6867..7811)
                     /locus_tag="PLI11_00445"
     CDS             complement(6867..7811)
                     /locus_tag="PLI11_00445"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HPZ51368.1"
                     /translation="MRVKRLFYHKAIVFLLAICLIVAFPLSSCTKKNAVSDETNLRGI
                     YQGKDLKADFSLRVNITDTKKSVFTIALIDGVFDISYSSGVYHCTLSSLQARLDSTDA
                     MMDYDSANTYSDEATIVRIAPYSSFLGVTFKATVDATGNITSLSGFNQVEENIKKSLD
                     DYGNTVVEQAIFAAKEYLDESVIKLILGFFIGYIPEKGLDVDKNWEYNGKIKAPFMLN
                     TNVSCTFNKVDEGKFIISTAGTLKTAIDEKNYSGIEYALTGTSESKIHLSKTGKVLRN
                     GSYTESVTGYCYYTIDGSDIKDTITMTRNISFSMREKE"
     gene            complement(7875..8858)
                     /gene="dusB"
                     /locus_tag="PLI11_00450"
     CDS             complement(7875..8858)
                     /gene="dusB"
                     /locus_tag="PLI11_00450"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPZ51369.1"
                     /translation="MMIRENFKIGNVKLNNNVFLAPLAGATDKSFRILSKEMGAGLVV
                     TEMISAKGVFYNNKNTLDLFEHTDMEKPISVQIFGSEPDVMADAAKYFEQSGADIIDI
                     NMGCPTPKITAGKSGSYLMTEPKLAGLIIEKVAKAVSIPVTVKIRRGYKTGTENAPEI
                     ARIAEESGCDAIAVHGRFADQFYSGHSDWEVIKRVKETVNIPVIGNGDIKCAEDAKRM
                     FEETKCDAIMVGRGALGNPWIFKEVLTCLKDGKVLEKPSKDEVLSMIVRQLEMTIEHK
                     GEYTGLREMRKHIAWYLKGFKNSSKMRDLVFRCEDKLKIVELLNIYFNESL"
     gene            9012..10337
                     /gene="glnA"
                     /locus_tag="PLI11_00455"
     CDS             9012..10337
                     /gene="glnA"
                     /locus_tag="PLI11_00455"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPZ51370.1"
                     /translation="MSYTKEDILRKIKEDDIRFIRLQFVDIFGIQKNVAIMSSQAERA
                     LNNSCMFDGSSIEGFVRVEESDMYLWPDLNSYVRFPWCTGGNVARFICDIYKSDRTPF
                     EGDPRYVLKKVVKEAESMGYTFNVGPELEFFLFHKDENGSPTTKTHDDAGYFDLGPVD
                     LGENARRDICLTLEDMGFEIEGSHHEVARGQHEIDFKYADAITAADNIMTFKLVVKTI
                     AQKYGLHATFMPKPIYGIAGSGMHTNMSLLKNGKNAFADENDKLGLSKDAYHFIAGIL
                     KHIKGFTAIANPIINSYKRLVPGYEAPCYITWSAQNRSTLIRIPSSRGEGTRIELRSP
                     DPSANPYLLFAVLLAAGLDGIKKELMPNESNNGCIYDLSDDELENMGIDKLPETLDRA
                     VECMIEDPLIRQTLGDHIFNKYVEAKKAEIYEYRTRVSSWEIDRYLSKY"
     gene            10388..11224
                     /gene="dapF"
                     /locus_tag="PLI11_00460"
     CDS             10388..11224
                     /gene="dapF"
                     /locus_tag="PLI11_00460"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPZ51371.1"
                     /translation="MNFVKMHGLGNDYIYVDLMKEHVEDPVNLARKVSDRHFGVGSDG
                     LILIDKSIVADFRMDIYNADGSRAEMCGNGIRCVGKYLYDKGYAKRKNIKIETLAGIK
                     ELELIIEDGVCTGARVDIGEPELNVRKIPVIWNDDTMIDQEILVGGKPYGVTCVSMGN
                     PHCVVFVDDVENIALEAIGPLFENHHLFPARINTEFAKVLDRKTIRMRVWERGSGETF
                     ACGTGASATVVAGVLNGLCDREATVILNGGELKILWDEASNRVYMEGPAVTICEGKYF
                     LD"
     gene            11268..>11696
                     /locus_tag="PLI11_00465"
     CDS             11268..>11696
                     /locus_tag="PLI11_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51372.1"
                     /translation="MLKRAVIFSFALVALFAIAGCNGDKKPKRGKNEVVVISYNVDAD
                     ESTLINRGPLMTDLINTLYPDSVGFQEARPGWLHLLTKNLKAYSYVGVSADGSNPHPG
                     SFGNYIFYLKDKYEVVLSGNYWHSDTPLIPSIYPGADCNRY"
BASE COUNT         3409 a         2169 c         2525 g         3593 t
ORIGIN      
        1 gcatggtggc ggtgcattct caggaaagga tccgactaaa gttgaccgct ccggtgcata
       61 tatggcaagg tatattgcaa aaaacattgt agccgcagga cttgctgaca aatgcgaaat
      121 acagattgca tatgccatag gtgtggcaag gccagtgtct gtcaggattg aaaccttcgg
      181 aacagaaagg gcagaagttt caaagttaga acaggcggtt attgacatat ttgacatgag
      241 accaagctcg attatcaatg agttcaattt aaggtctcca ctctatggaa aactggctgc
      301 atatggacac ttcggcagaa ctgatctttc tcttccatgg gaaaatacag acaaggtgga
      361 tttacttatt gaaaaggtta ggaattcatg attccatctg taggaagatt aacaaactca
      421 aaaaggaagc agtatttgaa gtattgtgaa tacgtggcaa agagcacaaa gagaataata
      481 gagccctttg cctcttttct atatttttct cttttatgtg ctgaaaaggg atttgaggga
      541 gatttttggt ttgattttaa tgatgaaata ttggctcacc tttttgggtt gattataaag
      601 tctcctgagt ttgtgtctga cggttatgag aaactgtggt cttgtcagtt tgattttgac
      661 aaggacaact attttaattg ccttaaagaa aaatacgaat atgttgattt tactgaaaaa
      721 gcagtgattc tatccttttt gatacaaaaa agcaaaaagg atcttacgga agataatgcc
      781 acatatggta gaaaacctaa aagtatgaga gaaactttaa aaagaatatc atttcttttg
      841 aaagatagga ccaaaataac tgtaaaggat attgatgttt ttaaaggcag ggcagaggat
      901 acatatttcc ttgaaaacgt gacttgtctt gataaaaatg tacaaaaaat gattgaaaat
      961 ggtgcaggat tcataatggt cctggaaaag gacttgggag agaaaaaaag aaacaatctt
     1021 gtttctaagt tgaagagtac aaccaacata cattttgatt atataaacaa tgtaatctgt
     1081 tcaaaggggg ctggttaaat gagcacgcca aaagcggaac ttgaaaaaag gatattgaat
     1141 ctttatgatg tgatgaagga taaggattac gatactataa taatcataaa caggataaat
     1201 caatactatt ttaccggcac aatgcaggat ggagttcttg tattaagaaa agacggaaca
     1261 gtaatgtttt ttgtcaggag aagctatgac agggcaaagc tggaatgtcc tttggacttg
     1321 gtttatccca tgaagacata caaggacatt ttggattatc tgcctaatga gcttggtgtt
     1381 gtgtacatgg aaacccagac ggtaacgata gctatcaagg agagacttca aaaatatttc
     1441 aagtttgatg ctgtaaagtc cattgacaag gaaatactcg tgctgagggc tgtcaaaagt
     1501 gattatgagc tggaatttat taaagagtct ggaagacagc acaattattt aatgaccagc
     1561 atcataccat caatattgag agagggaatg agcgaaacgg actttctttc cgaactttac
     1621 actcacatgg tgaaactggg atatcatggt atatccagat tcaacatgtt ccagatggag
     1681 atggttattg gtcagacagg atttggagac aactcaacgt atcctacaaa ttttgacggt
     1741 ccaggaggca tgaaaggcat gagtcctgct gtccccataa taggggatag gaacagatat
     1801 cttaagaaag gtgatattgt gtttgtggat gttggttatg gtgtggatgg ataccacagt
     1861 gacaaatccc aagtctactc cttcaaggca aaacccgatg acagggcact ggagattcac
     1921 agagcctgta tgatagctca aaagacaaca gcagacatga tgaaagaagg agcaatacca
     1981 tcggagatat atgaggaagt tatggcctct ttgccagatt gtcttaatga gaattttatg
     2041 gggatgtatg atcctgtaag gtttttaggt catggagtag gtttgcatat agatgagtta
     2101 cccatcatcg ccaaaggatt caatatgcct ttgaaacaaa acatggttgt aagtctcgaa
     2161 ccgaaatgtg ctgtaaaaaa cataggcacc gtcggtgtag aggaaaccta tgttataggt
     2221 agggacggtg ccgaatgtat cactggtgga gcttgtgata taattgttgt agattaatgt
     2281 tttattttat tgattttgaa accattcggt gttatggata catgataatc cattttaccg
     2341 gatggtattt ttccttctgc cgcataaaga ggagtgggga ttggactgaa tttgtagtcc
     2401 gtgaatccga ctgtcagagg agatatgcca aggacatatt tgtagtaaaa gattacagga
     2461 atggccgacc agccatggca aagagaacca gcattggaaa agtcccatgc tccatttatt
     2521 gtctcccaga aggtggtcgc attgttgtaa agcatgtatc cccatttgtc tgtaatttca
     2581 tcgaatacgt acttcccata ctcgtcactt actgaaagca gggcttcata cttgaaaatg
     2641 ctgtggctta ttgtaattgg caccaaatct tttccgtggg caagcatatg ggccactttc
     2701 tcctttttac tttcagggac caaacctgca tacaatgcaa gggcatttgt aagttcagaa
     2761 tagtgatgtt tttcgccgtt gataaggtaa gttgcataaa cttctttctc ttcatcccag
     2821 aaggttttgt caaaattatt caacacacct gcagccatca tgtcgtacca ggcttcaagg
     2881 tcgtgataat ttgcgtcctt tgtgtaatct gcaagattct ttgcaatctg cactgcagaa
     2941 cgcagggcaa gataatagaa ggcgttaagg ggtgcatcga aatttagcgg attatccttg
     3001 tcgcgcatgt tgtttaagga cctgtcccca gccaaaccat cggaccactc atagaagttc
     3061 cagtacttgt cgttgtaata cggtggtacc aaatcgcttc ccctggagtg aatccatacg
     3121 gatctgatta tttgctccac atgcttccac atctcctggg caaaatcaag atcgcctgtg
     3181 aaaagtatgt gctcatagag ttgtacaatc cacatcagtg taaagcaggg gattgtaatt
     3241 ggtatttcag caggtgcgca aagctccaaa aagccatcat ctctctggct caatcccaat
     3301 agtctaatgc tttcccttgc aaaatcatac tctccaaaag catagtagcc gcacagcatc
     3361 tggtttcttg agtccatggc gtaaagagcc tgctccctcc aagagcagtc ctcataatgc
     3421 tcatgcatgc aaaggagaag ggttctcaag cttatttcat atattttgtt gtgaagtgaa
     3481 tcggagcatg caaacactcc cttgtattca attgggtagt tcgttggtct aaggcccgca
     3541 tagtacaagg tgaattcctt tgcttccaca tacagttgga tatatctgca gccaaagcgt
     3601 tttatataat gggtgaattg atctcttttc ccgctggcgg tgagtttgcc ggaaaagttt
     3661 ctggttccca cataggatct gaccctcaaa tcatccagat gctctccata tccaatatta
     3721 ataactgttc cttcaggagc tgttatatct atttcaaaaa ctccgctttc ttctctgtgc
     3781 aaatcaagca caatatagat gccatcgtca tcccctgagt tgttcttgaa aataagcccg
     3841 ttttcttttg gaagggtggt gcatggagca tctccataca gaacatgagc atccctgtag
     3901 gagaggtatg aaaactgcat ttttgcagct gttgtttccg gttccgcgtg tgcactgtat
     3961 ttatatacgc cctgagctac aattttaggc tcgattctgt ctccaagttc aagctttttt
     4021 ataggtcttg gaaaaagctt ttgagtattt tcatacgcat aagccttgcc ccatgccatg
     4081 tctcctacca tggcatattc atatccttcc tggttccagt tgtcataacc gttgaaatca
     4141 aactcaaata caaagctcag ctgaggggtt gtaaggggca aagttccact catataatca
     4201 ggacttatcc tgcacagtgc gttgccactg ctgtacaata cggtgctatt atcttgttca
     4261 acaccgtata ttactccggg caatcctctt ttatatacag aggtgctgcg tccctgatga
     4321 taggtgagta tgcataatgt gttcttttct ccaatatcgt ttacaaggct tgtaatgtca
     4381 agactgtcat aaaccttgta ttcggggaaa tccgcatact gtccgtatcc ggcaagtttt
     4441 ccgttgaccc aggcaacgta ttcactgtcg gcactaatgt gtaatacaat tttctgaggt
     4501 tcttctgtta tggtgaacga gtccatatat tgtgtataaa tattctcgta cttttcatcc
     4561 ctggctgcgt ttacccacaa ccatttgcag ttagtaaagt taaaattatt attatccatt
     4621 tcattacctc tcttctacat gttgtcctca atcacgcact gtattatatc ttagataatt
     4681 gaatacttca acattgacag tgtttgataa agtcatcaaa aatgacttta tgagtcttgt
     4741 acttatgtgc cagcatttca ggatgccact gaactcccac tacatatctt ttttcaggca
     4801 tgtaaacagc ttcaatgaca tcatcgttgg aaacagccat tacggacagt cccttgccaa
     4861 ggtcttttat tgactggtgg tgtcttgtgt ttaccattat tatatttttt ttaatgattt
     4921 tatacagtgg tgagtctttt agtattgtta cttcatgtcc tccctcaaga agtggtctta
     4981 aatccctgtg attaatatca agctgggttg gaaggtcctg gtatagagtg ccttcaagag
     5041 ctacattcag aacctgtacc cctctgcata ttgcaaggag tggtttgtct tcatctattg
     5101 ctctttttat gagttttagt tcaaaggcat cccgtatttc atcaatttcc actgtatcgt
     5161 tgagtatatc ttctccaaaa tattttggat gtatgtcaat tcctccagta aacagaaagc
     5221 cgtcacagca tttcatcagc cagtcaaaat gttcttcatt ttcaacagga ggaataagta
     5281 atggaagacc tccggattgc ataatggagt ggcagtatga gccttcgata ctgccttccc
     5341 aaactgttgg ttgtctgttt gatgtaactc cgattactgt cttgttcatt atgtaataac
     5401 tccctttttt acatattata ttccaatatt tttaaaaaga ctagttgggt ttgtgcaaat
     5461 atgatatata ttatattgag aatatattta aggagtggta ggaatggaaa agattgaaag
     5521 aacaatcagg aatcttggaa aacacaacat aaaagggtat tttgtagaat ccaaagagga
     5581 tgtgacaact cttcttgaaa caatggtaaa agagggggag aaggtaacgg ttggaggttc
     5641 tcaaaccctt tttcaatgtg gtgttatcga ttggcttaga aatgggaagt ttgattattt
     5701 agacagatat gatgagaata aaaccaggga agaagtattg gatgtgttca gacaggcatc
     5761 cttttgcgac agctacataa ccagtactaa tgcccttact gaagaagggg aaatgcttaa
     5821 tgtggacgga cactgcaaca ggatttctgc aattgttcat ggtccaaaac aggttgttgt
     5881 gattgtcggg ataaacaaga tagtcaaaga tttggattag gctgttctgc gtgtgaaaga
     5941 gtttgcagct ccattgaatg caaaaagact taagaaaaac acatattgtg cggaacatgg
     6001 aagatgcata tctatagagg atgagaaaac aaaatacaag ctgggagcag gatgcgactc
     6061 cgaagacaga atttgctgta attatgtggt gtcctcctac cagcaggtta agaacaggat
     6121 aaaggttgta attgttggag aaagttttgg ttattaatct ggaacatagt tttcgaatat
     6181 gatattgttg caatactttc ttgcctgcgt ttcttcttcc ttactcctta tggtatatcc
     6241 aagaacaggc aatccaaact ttttgtagaa ggatactacg tttctgggca ggcatttaaa
     6301 atcgtatgta atgaatgatg gccttactat agggtttcca aacatgtttc ttgaaacaaa
     6361 tctttgtatt tgaggaatgt catcggggaa gcttgcggtt gccagttgtc cccgttttat
     6421 ttttggactg tatctcttaa aatgatagag gtaatcaggg taaaaggact gcacagctac
     6481 cttgcctttg tatgtttcga gcatctcgga aaggtttttt gctatctctt ttggtctacc
     6541 gcattttttt atctctatca agagaggaac ctggccatca acaagaacga aaacttcctc
     6601 caaggttgga atatgttcgt ttgtatctgc aagccttagt cttcttatct cttctttact
     6661 gctctcatta ataggcttgt caaaaccggt cattctcttt aagttggcat catggaagac
     6721 gtagatattg ttgtcggaag caagttgaat gtcaatttca ataggaaagc cataatcaat
     6781 ggcattttta aaagcagata atgagttttc agggtatttt ttgttatgta atcccctatg
     6841 ggcaataggt ttcgtcaaaa attccttcat tctttttccc tcatcgaaaa tgatatgttt
     6901 cttgtcattg tgattgtgtc ttttatatca ctgccatcga tagtgtaata gcagtatcct
     6961 gttacacttt cagtatatga tccgtttctc aaaactttcc cggttttcga aagatgaatt
     7021 tttgattcgg aagttccagt aagcgcatat tcaattcccg agtagttttt ctcatctatt
     7081 gcggttttca acgtccctgc agttgatatt atgaatttgc cttcatctac tttgttaaac
     7141 gtgcatgaaa cattcgtgtt cagcatgaaa ggtgccttta tctttccgtt atattcccag
     7201 tttttatcta catcaaggcc tttttccgga atatatccta tgaaaaaacc aagaattaat
     7261 ttaataacgc tttcatcaag atattccttt gcagcaaata ttgcctgttc cacaaccgtg
     7321 tttccataat catcaagtga cttcttgata ttttcctcaa cctggttgaa cccactcaag
     7381 gatgtaatgt tacctgttgc atccacggtt gctttgaagg taacaccaag gaaagaactg
     7441 tatggagcaa tccttacaat ggttgcttcg tcggaatatg tatttgccga atcgtaatcc
     7501 atcattgcgt ctgtggaatc aagtcttgct tgcagggaag acagggtaca atgatagact
     7561 ccggagctgt agcttatgtc aaatactccg tcaatcagtg cgattgtaaa aactgatttc
     7621 tttgtgtcgg tgatatttac tcttagtgaa aagtctgctt ttagatcttt tccctgataa
     7681 atgcctctta ggttggtttc gtcactgaca gcattctttt ttgtgcatga agatagaggg
     7741 aatgcaacaa tcaagcagat agccaacaaa aaaactattg ccttatgata aaacaacctt
     7801 ttaactctca tgattccctc ctagctgaaa ttatatgttt attatatttc tgtaaaatat
     7861 taatgtcaag ttgtttaaag actttcatta aagtaaatat ttaggagctc aacaatctta
     7921 agcttgtcct cgcatctgaa aacaaggtct ctcattttgg agctgttttt aaaacctttc
     7981 agataccagg ctatatgttt tctcatttcc cgaagacctg tatattcacc cttgtgctca
     8041 atagtcattt caagctgcct gacaatcatt gaaagaactt catctttaga aggcttctcc
     8101 aagacctttc cgtctttaag acaagtaaga acttctttga aaatccatgg attgcccaaa
     8161 gcacctcttc ctaccatgat tgcatcgcat tttgtttcct caaacatcct ctttgcatct
     8221 tctgcgcatt ttatatcacc attgccaatc acgggtatgt tgaccgtttc cttcactcgc
     8281 tttatgactt cccagtcgct gtgtcctgag tagaactgat ccgcaaacct tccatggact
     8341 gcgattgcat cgcagccgga ctcttctgca attcttgcaa tttcaggtgc gttctcagta
     8401 cctgttttgt accctctcct tattttcaca gtcacaggaa tagaaacagc tttggccact
     8461 ttttcgataa tcagtcctgc cagtttcggt tccgtcatta agtatgagcc gctttttcct
     8521 gcggtaattt taggggtagg gcagcccatg ttaatgtcta ttatgtctgc accggattgc
     8581 tcgaaatatt ttgctgcatc cgccattaca tcgggttcgc ttccaaaaat ctgcacgctg
     8641 ataggttttt ccatatcagt atgctcaaag agatccagtg tgtttttgtt gttgtagaaa
     8701 actcccttgg cgcttatcat ttcagttacg acaagacctg ctcccatttc cttgctgagt
     8761 atgcgaaagg atttgtcagt agcgcctgca agtggtgcaa gaaaaacatt gttattcagt
     8821 tttacatttc ctattttaaa attttctcgt atcatcataa acatcctaag cattaaatgt
     8881 accgaagaat tttagcagaa aggcatattt catgtcaaat tcaacgcagt agattacaca
     8941 tttacaatca ccatataatt tggtataatt tatgagccct ccaagggtaa aataaggaag
     9001 aggggaatac cgtgagttac actaaggaag atattctaag aaaaatcaag gaagatgaca
     9061 taagattcat cagattacaa ttcgtggaca tctttggaat tcagaaaaat gttgcaataa
     9121 tgtcaagtca ggcggaaaga gcattaaaca attcatgcat gtttgacgga tcctccatag
     9181 aaggttttgt aagggtggaa gagtccgata tgtatttgtg gcctgatttg aattcgtatg
     9241 ttcgttttcc ctggtgtaca ggtggtaatg tggccaggtt catatgcgat atatataaat
     9301 cagatagaac tccgtttgaa ggagacccca gatatgtttt gaaaaaggta gttaaagaag
     9361 ctgaaagcat gggctatact ttcaatgtgg gtcccgaact tgagtttttc ttgttccaca
     9421 aggatgaaaa cggatctcca acaacaaaaa ctcatgatga tgcaggctat tttgacttgg
     9481 gacctgtgga tttgggcgaa aatgcaaggc gggatatatg cctaaccttg gaggatatgg
     9541 gatttgagat tgagggatcg catcatgaag tggctagggg tcagcatgag attgatttca
     9601 agtatgctga tgctattact gctgcagaca acataatgac attcaagctg gtagtcaaaa
     9661 ccatagctca gaagtatgga ctgcacgcaa ctttcatgcc gaaacccata tacggtattg
     9721 caggttctgg catgcatacc aacatgtccc tgctcaaaaa cggcaagaat gcttttgcgg
     9781 acgagaatga caagctgggg ctaagcaagg atgcatatca ttttattgct ggaatactca
     9841 agcacataaa gggctttaca gccattgcaa accctattat caattcatac aagagactag
     9901 tacccggata cgaagcaccc tgctatataa cctggtccgc acaaaacaga agcacgctta
     9961 ttagaatacc ttcatcaaga ggtgaaggca ctagaataga attgagaagt cctgaccctt
    10021 ccgcaaatcc gtatcttttg tttgcggtgc tgctggcagc aggtcttgac ggcattaaaa
    10081 aggaacttat gccgaatgaa agcaacaatg gatgcatcta tgaccttagc gatgacgaac
    10141 ttgagaatat ggggattgat aaacttcccg aaacgcttga cagggcagtt gaatgcatga
    10201 ttgaagatcc attgattaga caaactcttg gcgaccatat attcaacaag tatgttgaag
    10261 ccaaaaaggc ggaaatatat gagtacagga cgagagtgtc ttcgtgggaa attgacaggt
    10321 atctgtcaaa gtattgaccg tatgttcata agatatatta tatctgataa gcaagggggc
    10381 atctgagttg aatttcgtaa aaatgcatgg tcttggaaac gactatattt atgtcgacct
    10441 aatgaaggag cacgtggagg atcctgttaa tcttgccagg aaagtgagtg acaggcattt
    10501 tggagttggt tctgacggtc tgatcctaat agataaatcc atagttgctg attttagaat
    10561 ggatatctac aatgcggatg gttcaagggc tgaaatgtgt ggaaacggta tcaggtgtgt
    10621 gggcaaatac ttgtacgaca agggttatgc caagaggaag aatataaaga ttgagacact
    10681 ggcgggcata aaggagctgg aactcataat tgaggatggt gtttgtacag gtgccagagt
    10741 tgatattggc gagcctgaat tgaacgtaag gaagattcca gttatatgga atgacgatac
    10801 catgatagac caggaaattc ttgtaggagg taaaccttac ggtgttacat gcgtttccat
    10861 gggtaatcct cattgtgttg tttttgtgga cgatgttgaa aatatcgccc ttgaggccat
    10921 tggtccattg tttgaaaacc atcatctttt cccggctaga atcaacacgg agtttgcaaa
    10981 agttttggat agaaaaacaa tcagaatgag ggtttgggaa agaggttcag gtgaaacctt
    11041 tgcctgcgga accggtgcca gtgcaacagt ggtggctgga gtattgaatg gcctttgcga
    11101 cagggaggca actgtaattc ttaacggagg agagctcaag attctttggg atgaagcatc
    11161 aaaccgtgtc tacatggaag gtcctgccgt tacaatatgc gagggtaaat actttttgga
    11221 ttgattgtgg tataatagcc ttagcataag caaaggacgt gttggtgatg cttaaaagag
    11281 cggtaatttt ttcatttgct ttggtggctc tttttgcaat tgccgggtgc aatggcgaca
    11341 aaaagccaaa gagaggaaag aatgaggtag ttgtaatcag ctacaatgtg gatgctgacg
    11401 agtcaaccct gataaataga ggacctttga tgacagattt gataaacacc ctctatcccg
    11461 attcggtagg ttttcaggag gcaaggccgg gttggctgca tcttcttact aaaaatctta
    11521 aagcttattc ttacgtggga gtgtctgcgg atgggtctaa ccctcatccg ggatctttcg
    11581 ggaattatat tttctatttg aaagacaaat atgaagtggt tttatctgga aactactggc
    11641 attccgacac tcctttgatt ccttcaatat atcccggagc ggactgcaac aggtac
//
LOCUS       DAONVY010000008        11464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_4358_length_11464_cov_3.970550, whole genome shotgun sequence.
ACCESSION   DAONVY010000008 DAONVY010000000
VERSION     DAONVY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11464
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4358_length_11464_cov_3.970550"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLI11_00470"
     CDS             complement(<1..688)
                     /locus_tag="PLI11_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPZ51373.1"
                     /translation="MSEQINNRQYRQNVIKELIRELHEGKTVDEVKHKFEQAFAGVSA
                     TEISEAEQALINEGLPVEEVQRLCDVHAAVFKGSIEEIHASIDVSEIPGHPANVLKVE
                     NMYIDVLIDSKLMPVLVNKSDPNFRKNLDEALKELYQVDIHYAKKENLLFPYLEKYGV
                     TAPPQVMWGVDDEIRDEIKDLRREIQSDKSDVEDIVERGMELAEKIKDMIFKEENILL
                     PMLVDMLTEDE"
     gene            complement(888..978)
                     /locus_tag="PLI11_00475"
     tRNA            complement(888..978)
                     /locus_tag="PLI11_00475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(941..943),aa:Ser,seq:tga)
     gene            complement(1051..1551)
                     /locus_tag="PLI11_00480"
     CDS             complement(1051..1551)
                     /locus_tag="PLI11_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HPZ51374.1"
                     /translation="MITGNINMDIIFGVLFLLILINTILVVVLFKKLSHIRKSQKVLN
                     ELFSGESVEELLYKVLEQQEIISNEALDIKKQILQLENKQKYCFDRISLVRYRGTQED
                     GAKLSYSIGISNENEDGLIITGIHFRNGVNLYFKHVKEGMPDIELSEEEKKVLARTKV
                     NEVYSE"
     gene            complement(1589..2461)
                     /locus_tag="PLI11_00485"
     CDS             complement(1589..2461)
                     /locus_tag="PLI11_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPZ51375.1"
                     /translation="MSRRKTGLGKGIDALIPSGHEELSDSVIEIDIHDIEPGDAQPRK
                     SFDEEKIAQLAQSIKEHGIIQPLIVMKEGSVYKIIAGERRWRAARLAGLKKVPAIERS
                     ATPQEVMELALIENIQREDLNPIEEAEAYSRLMTEHNITQERLSEIIGKSRSAIANTI
                     RLLNFNDQIREMIINEEITAGHARAILALESEDERVRVAREVVDKGYNVRQTEDLVKK
                     LLQEKAKKKENKDITDENNTFSQVEIKHIQNMLRSVLGTKVKMDEAKGRGKIIIEYYS
                     PDERERLIDYLTKG"
     gene            complement(2481..3242)
                     /locus_tag="PLI11_00490"
     CDS             complement(2481..3242)
                     /locus_tag="PLI11_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ51376.1"
                     /translation="MAKVIAVANQKGGVGKTTTAINLSSCVAHRGKRVLLIDLDPQGN
                     TTTGLGINKRNLDKSIYDVIINEENIENVLLDTHQNTLNLCPSSMKLAGAEVELVSVI
                     SRETRLKYALEDVEDIFDFIFIDCPPSLGLLTINALTASQTVLVPIQCEYYALEGLTQ
                     LMDTVKLVQKRLNRDLEVEGVVLTMFDARTNLSIEVVDEVRKHFKDKVYGTIIPRNVK
                     LSEAPSYGLPIVLYDEKSKGAQAYLELADEFIALA"
     gene            complement(3410..5338)
                     /gene="gyrB"
                     /locus_tag="PLI11_00495"
     CDS             complement(3410..5338)
                     /gene="gyrB"
                     /locus_tag="PLI11_00495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPZ51377.1"
                     /translation="MAENINTNRYDENDIQVLEGLEAVRKRPSMYIGSTSIRGLHHLV
                     FEIVDNSIDESLAGRCDTIKITINSDNSVTVSDNGAGIPVGIHKQRGIPTVEVVHTVL
                     HAGGKFGGGSYKVSGGLHGVGASVVNALSEFMEVEIKREGHIYYQRYERGKVMCPLKV
                     IGETTETGTTSTFKPDHLIFETIEFDKNILLARFREMAFLNAGIKIILKDNRLEEPYE
                     TVLHYEGGIKSFVEYLNRKETVLNNPPIYISGEKEDAIAEIAIQYNDNYVENIYSYAN
                     NINTQEGGTHLTGFKSALTKVFNDYGRKHNLLKDSEKNLAGEDIREGMTAIISVKLKE
                     PQFEGQTKQKLGNSEIRGFVESIVNEKLAAYLEENPQVAKAIFDKCLTAQRAREAAKR
                     ARELTRRKNALESFSLPGKLADCTEKDPTLCELFIVEGDSAGGSAKQGREREFQAILP
                     LWGKMLNVEKSRIDKVYNNDKLLPVILSIGANVGPDFDVEKVRYHKVIIMADADVDGS
                     HIRMLLLTFFYRYMKPLIEKGYLYIAQPPLYKVSKGKEEFYAFDDKDLEKRISERGWT
                     KNDKNIVIQRFKGLGEMDHDELWETTMNPETRTLLQVDLINAATANEIFSDLMGEKVE
                     PRREFIYENAKYATNLDF"
     gene            complement(5342..5638)
                     /locus_tag="PLI11_00500"
     CDS             complement(5342..5638)
                     /locus_tag="PLI11_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPZ51378.1"
                     /translation="MFIHIGDNIIIPVDEIVGIFDLEGTTITGITRDFLKVAEEEGFV
                     IATSSRMPKSFVITVRNNQNLIYLSSLSVNTLKKRVKKWLDMLNNKTVRLVRGS"
     gene            complement(5725..6840)
                     /gene="recF"
                     /locus_tag="PLI11_00505"
     CDS             complement(5725..6840)
                     /gene="recF"
                     /locus_tag="PLI11_00505"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPZ51379.1"
                     /translation="MKTLIVDSIRLENFRNYKDIELNFCDGINIFQGLNGQGKTNLLE
                     SIYICSVGKSFRTSKDIEMINHEKEYFTVNVGIKNDATDKIFIRYDRQKQKAIAVNGI
                     FLRKSGQLMGALLCVLFCPEDLLILSDGPSRRRRFLDIAISQLNPLYYYNLQQYTKLT
                     AQKNVQLKQIKKNPKQKDVLFVWNEQIAEYGSKIIAERIKFIDVLKDLANESHAYISE
                     YKEQLVLEYENTIELNEYDERHIKETYIKTLEKIMQREIEREVSLIGPHRDDISFRLN
                     DLELKKYGSQGQKRTAVLSLKIAEIEIMKKNTGRIPILLLDDVMSELDKKRQEALLSY
                     VKGVQTFITCVDSESFDQLKDTQNYIFNISDGAVSKL"
     gene            complement(6821..7057)
                     /gene="yaaA"
                     /locus_tag="PLI11_00510"
     CDS             complement(6821..7057)
                     /gene="yaaA"
                     /locus_tag="PLI11_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HPZ51380.1"
                     /translation="MGETKILINTEYIKLDQFLKWCGIADTGGTAREIITGEDVYVNG
                     EIETRRGRKLRPGDRVDIFGRTFVVSASGEDIDS"
     gene            complement(7083..8183)
                     /gene="dnaN"
                     /locus_tag="PLI11_00515"
     CDS             complement(7083..8183)
                     /gene="dnaN"
                     /locus_tag="PLI11_00515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPZ51381.1"
                     /translation="MKFTCNRDNLVEAINVVQRAISGKTTMPILEGILIEVIEELKMT
                     ANDLDLGITYEIPAIIEEVGSVLVSSKTFGDIVRKLPDIYVTIETSEANNLVIITSGK
                     ARFEISMMDIDEYPQVNFIDEEESVTFENEILRSLIKQSIFATADDDSKPMFKGVFID
                     KEGKTVNFVALCNSKMAIKHTSMENEKEFKILVPTRILNEVYKVLQITNENMSMTWSK
                     TQVMFYNSHFKIISRLINSEYLKYRDLIPREDKLRVVVSTKDFLHAIERSALVMQDSS
                     KYPMVLTTDMDEIVISAKAERGHSEERLPAQVNGSSLTIGFNTSNFIDCLKVIDDETI
                     SLHFTTSLGPCIVRGEEYDDYVFLVMPVRLKL"
     gene            complement(8456..9769)
                     /gene="dnaA"
                     /locus_tag="PLI11_00520"
     CDS             complement(8456..9769)
                     /gene="dnaA"
                     /locus_tag="PLI11_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPZ51382.1"
                     /translation="MTDSLESIWKKCLNTLSADIQPTLFNAYIMSITPLEITEDIIAF
                     EVPEAFLLNTIENKFYPQIRNAITLHTGKEYRLHFYLPDQYKKKEQENKQIIPRFNSH
                     LNGRYTFDSFITGNSNRMAYAAAVAISSSPGKAYNPLFIYGQSGLGKTHLMHAIGNEI
                     LNKDPNQKVLYVTSETFTNEFIEALEQKKNDEFRNKYRKIDVLLIDDIQFISRRERTQ
                     EEFFYTFNSLYSDGKQIVVSSDRPINEISILEDRLRTRFAWGLIADIQAPDFETKVAI
                     LKRKADENNIIVEKNILEHIAFQTGDNIRELEGIINHLTISSSQGRKIDLNLAREALR
                     HLQKTETKEITPKVIIDVVSRYYNLTVEDILSKKRNKEIAFPRQIAMYLCRELSNLSF
                     PEIGEAFGGKNHTTVMYAHEQITKKCEESFDLRDTIEEIKKSIVC"
     gene            10234..10368
                     /gene="rpmH"
                     /locus_tag="PLI11_00525"
     CDS             10234..10368
                     /gene="rpmH"
                     /locus_tag="PLI11_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPZ51383.1"
                     /translation="MLRTYQPKKRKRSKLHGFRARMKTRGGRKVLKARRRKGRKILSA
                     "
     gene            10410..10799
                     /gene="rnpA"
                     /locus_tag="PLI11_00530"
     CDS             10410..10799
                     /gene="rnpA"
                     /locus_tag="PLI11_00530"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPZ51384.1"
                     /translation="MKNMVSLKTNREFSRVFKKGKYKAGKHIVVYALENGFSYNRVGI
                     SISKKVGIAVQRNRVKRLIRESYRLSEDSILTGYDIVILVKSTVRAAKTPNNRLKAVS
                     LPSFHDIDSEFKKLGMKLGIFKKEESD"
     gene            10801..11010
                     /gene="yidD"
                     /locus_tag="PLI11_00535"
     CDS             10801..11010
                     /gene="yidD"
                     /locus_tag="PLI11_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPZ51385.1"
                     /translation="MKRVLIWIIGIYKKYLSPLKRPCCRFYPTCSSYAIEAIEKHGAV
                     KGLVMSIWRILRCNPFNKGGYDPVK"
     gene            11066..>11464
                     /locus_tag="PLI11_00540"
     CDS             11066..>11464
                     /locus_tag="PLI11_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPZ51386.1"
                     /translation="MKLIYDTIAFQSYGLTLIYFTIFVRILLFPLNLKQQKSLLRNQE
                     LQPEIEELRRLYKNDKEKLNEEQMKLYQKYNVNPMAGCLPMLIQFPLIISLYRIVTQP
                     LTYISGVSASALTALRNFVAGAQNISNSKTV"
BASE COUNT         3244 a         2350 c         1845 g         4025 t
ORIGIN      
        1 actcatcctc ggtgagcatg tctacgagca ttggaagcag tatattttct tccttgaata
       61 tcatgtcctt tattttttca gcaagttcca ttcctctttc gacaatatct tctacatctg
      121 acttgtctga ctgaatttct cttctcaaat cctttatttc atccctaatt tcatcatcca
      181 caccccacat tacctgcgga ggagctgtta ctccgtactt ttcaagatat gggaaaagca
      241 gattttcctt ctttgcgtaa tgaatatcta cctgatacaa ctcctttagt gcttcatcaa
      301 ggttttttct aaaatttgga tctgacttgt tgactagtac tggcatcaat ttactgtcta
      361 tcaacacgtc aatatacata ttctcaactt taagaacatt ggctggatgg cctggaatct
      421 ctgatacatc aatgcttgca tgaatttctt caattgagcc tttgaataca gccgcatgga
      481 catcacataa tctctgtact tcttctaccg gcaatccttc attgattaat gcctgttccg
      541 cttccgatat ttctgtagcc gaaactcctg caaatgcctg ttcaaacttg tgttttactt
      601 cgtccacagt ttttccttca tgcagttccc ttatcaattc cttgattacg ttctgtctgt
      661 actgtctgtt gttgatttgt tcactcataa tttgatacct cgacttttct tttatttttt
      721 atatactact attattaacc aatatggata aataatatgt gatacaagtc acgcagctag
      781 aacggttttc tcattgatag ccatatttta catcaattta tttgagttgt ctaattttat
      841 ataaaaaaag tctggaaatc tttcttactg actttccaga ctaagtttgg cggaaagagt
      901 gggattcgaa cccacggtag gcttgcacct acgccggttt tcaagaccgg tgccttaaac
      961 cagctcgacc atctttccac tttatttttt tgacttgatt ataatatcat ttatttttta
     1021 aattgtcaag gacttgatgt taaaattcca tcactcggag tacacctcgt tgactttggt
     1081 ccttgctaat actttctttt cttcttctga caattctatg tccggcatgc cttctttaac
     1141 atgtttgaaa tacaagttaa caccatttct gaaatgaatg cctgtaatta taagtccatc
     1201 ttcattttca ttgcttattc ctatggaata acttaacttg gcaccatctt cctgagtacc
     1261 tctgtatcta acaagactga ttctgtcaaa acaatatttt tgtttgtttt caagttgaag
     1321 tatctgtttc tttatatcca gtgcttcatt tgatataatt tcttgctgtt caaggacctt
     1381 atacaaaagc tcctcgactg attcacccga aaacaactca ttaagtactt tctgcgactt
     1441 ccttatgtgc gacagcttct taaaaagcac aaccactagt atagtgttta ttaatataag
     1501 taaaaaaagc actccaaaga ttatgtccat atttatgttt cctgttatca taacttctct
     1561 ccttttctct ttgtttcacg tgaatcattt atccttttgt cagatagtct ataagtcttt
     1621 ctctttcgtc aggtgaatag tattctataa taatcttccc tcttcccttt gcctcatcca
     1681 tttttacctt agtccccaat actgatctaa gcatgttttg gatatgttta atctctacct
     1741 gcgaaaatgt attattttca tccgtgatat ccttattttc cttcttcttc gctttctctt
     1801 gtagtagttt cttaacaagg tcttcagtct gcctcacgtt atatccttta tcaacaactt
     1861 ctcttgcaac tctgactctc tcgtcttcac tctccaaagc tagaattgca cgtgcatgtc
     1921 cagctgtaat ttcctcattt attatcattt cccttatttg atcattaaaa ttcagcaatc
     1981 ttattgtgtt tgcaatggca gatctacttt ttccaattat ctcagataat ctttcttgag
     2041 ttatattgtg ctctgtcata agcctggaat aagcttctgc ttcttcgatt gggttcagat
     2101 cctcgcgctg aatgttttct ataagggcaa gctccataac ttcctgtggt gtagcgcttc
     2161 tttcaatagc aggaaccttt ttaagccctg ctaaccttgc agctctccat cttctttcac
     2221 cggcaataat tttataaacc gaaccttcct tcataacaat aagcggttgt attattccat
     2281 gttctttgat ggactgtgca agttgagcta ttttttcctc atcaaaactt tttcttggtt
     2341 gagcatcacc aggttcaata tcatgtatat ctatttctat aacactgtct gataattctt
     2401 cgtgacctga tggtattaaa gcatcaatcc cttttcccaa tcctgttttc cttcttgaca
     2461 cgtcgttacc tcactttctt ttatgctaaa gctataaatt catcggccag ttccagataa
     2521 gcttgagctc ccttagattt ttcatcgtac aatactattg gcaagccata gctaggagct
     2581 tcgcttagtt taacattacg aggtataatt gttccatata ccttgtcttt gaaatgtttg
     2641 cgcacttcat ccacaacctc tatggataaa tttgttctcg catcaaacat tgtcaaaaca
     2701 acgccttcta cttccaaatc tctgttcagt ctcttctgaa ccagttttac tgtatccatc
     2761 aactgggtaa gaccttcaag ggcatagtat tcgcactgaa taggcaccaa cacagtttgt
     2821 gatgcagtca atgcgtttat tgttaaaaga ccgagcgatg gaggacaatc tataaatata
     2881 aaatcaaaaa tatcctctac atcttccaat gcatatttca accttgtttc tcttgatatt
     2941 acacttacca gctcaacttc ggcacctgcc agtttcatac tggatggaca aagattaaga
     3001 gtgttttgat gggtgtcaag cagtacattt tctatgtttt cttcattaat aattacgtcg
     3061 tagatggact tatccaaatt ccttttatta attcccaaac cagtagtagt gttgccctgg
     3121 ggatccaggt caataagaag gactcttttt cccctgtgtg ccacgcaaga actcaaattg
     3181 attgctgtag ttgtttttcc aactccacct ttttgatttg caacagcgat aacttttgcc
     3241 attttgtact tcctttcttt tcttgtagga aaattatatc atatcacata tacactttca
     3301 atggaattga taaaaatatt ggttaaaatg tttcacgtga aacattttgt aaaacaaaac
     3361 cccctattgt ttcacgtgaa acaatagggg gtgcattcct ttaagttagc tagaagtcaa
     3421 gattggtagc atattttgcg ttttcataaa taaactctct tcttggctca actttttcac
     3481 ccataagatc tgagaaaatc tcattagctg tagcagcatt tatcaaatcc acttgaagaa
     3541 gagttctggt ttcaggattc attgtagttt cccaaagttc atcatggtcc atctcaccaa
     3601 gacccttaaa acgctgtata actatgttct tatcgttttt tgtccatcct ctttctgata
     3661 tacgtttttc aaggtccttg tcatcaaacg catagaactc ttccttgccc ttcgaaacct
     3721 tgtacaaagg tggttgtgct atatacaaat aacctttctc aataagaggc ttcatatatc
     3781 tatagaagaa tgtaagcaaa agcatgcgaa tgtgtgaacc gtccacatca gcgtccgcca
     3841 ttataataac tttatgatat cttacttttt caacatcaaa atcaggacca acatttgctc
     3901 ctattgaaag tattacaggc agaagcttgt cgttgttata tactttatct attctagatt
     3961 tttccacatt taacattttt ccccataaag gaagaattgc ttgaaattct ctttctcttc
     4021 cctgtttggc ggaaccacca gcagagtcac cctccactat gaaaagctcg cataaagtag
     4081 gatccttttc agtacagtct gccagtttgc caggtagtga aaaactctcg agtgcatttt
     4141 tacgtcttgt aagttccctg gccctttttg cagcttctct ggctctttga gctgtaagac
     4201 acttgtcaaa aatagctttc gcaacctgag gattttcttc aagatatgcg gccaattttt
     4261 catttacaat actctcaacg aatcccctta tttcactgtt gccaagcttc tgtttggtct
     4321 gtccctcaaa ttggggttct ttaagcttga cactaataat tgccgtcatg ccttccctta
     4381 tatcctcacc tgccagattc ttttcactgt ccttaagcag gttgtgttta cgtccataat
     4441 cgttgaaaac tttagtcagt gcagatttga agcctgtaag gtgagttcca ccttcctggg
     4501 tgtttatgtt gtttgcatag ctgtatatgt tttctacgta gttatcattg tactgaattg
     4561 ctatttcagc aatagcgtcc tctttctctc cagaaatgta tataggagga ttgttcaaga
     4621 cggtttcttt tctgttaagg tattctacaa aagacttgat acctccctca taatgcaaaa
     4681 ctgtctcata aggctcctca agtctgttgt ctttcagtat tatctttatg cccgcattta
     4741 aaaatgccat ttccctaaaa cgagcaagca aaatattttt atcaaactca attgtttcaa
     4801 atatcaagtg gtcaggttta aatgtggatg ttgtaccagt ctccgttgtc tctccaatta
     4861 cttttaacgg acacattacc ttgcctctct catatctctg gtagtagata tgaccttccc
     4921 tctttatctc tacttccata aactcggata gtgcattaac aactgaagca cctacgccat
     4981 gcaaaccacc tgatactttg tagctgccgc ctccaaactt tccaccagca tgcaatacag
     5041 tatggacaac ttcaacagta ggtatccccc tctgtttgtg aatacctaca ggaataccag
     5101 caccgttgtc tgatactgtt acggaattat ccgaattaat tgttattttt attgtgtcgc
     5161 atctaccagc cagtgactca tctatactgt tgtcaacaat ttcaaatacc agatgatgaa
     5221 gacctctaat tgaagtactt ccaatataca tactgggtct ttttcgaaca gcttcgagac
     5281 cttcaagtac ctgtatgtca ttttcatcat accggtttgt attaatgttt tccgccataa
     5341 actaacttcc ccttactaat cttactgttt tgttattcaa catatccaac catttcttca
     5401 cccttttttt tagtgtattt acagacaagg atgaaagata aatcagattt tggttattcc
     5461 tcactgttat tacaaaagat ttcggcattc tggatgatgt tgctattacg aagccttctt
     5521 cttcagcaac ctttaaaaaa tccctggtaa ttccggttat agttgtacct tccaagtcaa
     5581 agattccaac tatctcatct acagggataa taatgttatc gccgatatga ataaacaaag
     5641 ttccacctct gggtaattaa taaacaaata taaagatatt tgagtatata tattatagca
     5701 tgaatgggac ataattgcaa ttaactaaag ctttgaaacc gctccgtctg atatgttgaa
     5761 aatgtagttt tgggtgtctt tcagctgatc gaaagactca gaatcgacac aagtaatgaa
     5821 tgtctgaact ccttttacat aagacagcaa agcctcctgt ctctttttgt cgagttctga
     5881 cataacgtca tcaagtaaaa gaataggaat tcttcctgtg ttttttttca ttatttcaat
     5941 ctctgcaatc tttaaagaca atacagccgt tctcttctgc ccttgagagc catatttttt
     6001 tagttccagg tcattaagcc tgaaacttat atcatctctg tgagggccaa taaggctaac
     6061 ttccctttct atttccctct gcattatctt ttccagtgtt tttatgtaag tttctttaat
     6121 gtgcctttca tcgtattcat taagctctat tgtattctca tactccaaga caagttgctc
     6181 cttatactca gaaatgtatg catggctttc attggcaagg tctttcaata catctataaa
     6241 ctttattctt tcagcaatta ttttagagcc atattcagca atctgttcat tccagacaaa
     6301 aaggacatcc ttctgttttg ggtttttctt tatctgcttc agctgcacgt tcttttgagc
     6361 tgtcagtttt gtatactgtt gcaggttgta atagtaaaga ggattcaact ggcttatagc
     6421 tatatcaaga aatcttcttc gtcttgaagg gccatctgaa agaatcagca agtcctcagg
     6481 acaaaaaagg acacataaaa gcgcacccat aagctgtcca gattttcgta aaaatatgcc
     6541 attgacagct atagctttct gcttctgtct gtcatatctg ataaaaattt tgtcggttgc
     6601 atcattctta ataccgacat tcacagtaaa atattccttt tcatgattta tcatctcaat
     6661 atctttcgat gttctaaaag atttgccaac ggaacagatg tatatggatt ccagaagatt
     6721 cgtcttaccc tgtccgttca aaccctgaaa gatattaatt ccatcacaaa aattcaattc
     6781 aatatcttta tagtttcgga aattttccaa tcttatagaa tcaactatca atgtcttcac
     6841 cgcttgcgga aacaacaaag gttcgaccaa aaatatcaac cctgtcccca ggtcttagct
     6901 tccttcctct tcttgtttct atttcgccat taacgtaaac atcctcacca gtaataattt
     6961 cacgtgcagt accgcctgta tcggcgatac cacaccattt taaaaactga tctagtttga
     7021 tgtattcggt attaatcagt attttagttt cgcccataaa ttatcaaaac ctcttattct
     7081 aattacaatt tcaatctcac tggcattacc agaaatacat aatcgtcata ctcctcgcct
     7141 cttacaatac atggtcccaa actggttgta aagtgcaggg atatggtttc atcgtcaatt
     7201 accttaaggc agtcaataaa atttgaagtg ttaaacccta ttgttaagct tgacccgttt
     7261 acttgggcag gaagcctttc ttccgagtgc cctctttctg ccttagccga tattactatt
     7321 tcgtccatgt ctgtagtcag caccattgga tacttggaac tgtcctgcat aacaagagcg
     7381 gaacgctcaa ttgcatgaag aaaatctttt gttgatacta caacacgcag tttatcctct
     7441 ctaggaatta aatccctgta cttcaagtac tcgctattta tcaatcggga tattatttta
     7501 aaatggctgt tgtaaaacat cacctgtgtt ttagaccagg tcatggacat gttttcattc
     7561 gtaatctgca gtaccttgta tacctcgttg agtatcctgg ttggcacaag gatcttgaac
     7621 tctttttcgt tttccatgga agtgtgcttt atagccatct tgctgttaca aagagcgaca
     7681 aaatttactg tctttccttc cttgtcaatg aaaacgccct taaacattgg cttggaatca
     7741 tcatccgctg ttgcaaatat actttgcttt atgagacttc taagaatttc attttcaaaa
     7801 gttaccgatt cctcttcgtc aatgaagttg acctgtgggt attcatctat atccatcatg
     7861 gatatttcaa atctagcctt tccactggtt ataatgacaa gattgttagc ctcagaagtt
     7921 tctattgtaa cgtatatatc gggcagtttc cttacaatat ctccaaatgt ctttgaagaa
     7981 acaagtacag aacccacttc ttctattatt gcaggtattt cataagttat accaagatcc
     8041 aggtcattag cagtcatctt aagttcttct ataacttcta tcaatatgcc ttctagtata
     8101 ggcatggttg tttttcctga tatagctctc tgcactacgt taatagcttc taccaaattg
     8161 tctctattgc atgtgaattt catatggcat cctccctgta tgtattatta tatatatttt
     8221 tttttagtag tattagtagt aggggttgtg caaaatgtgg ataagtattt ttcctactat
     8281 tatatatatc atttttttgt ctgtgaaaca actgtcaata acttggggat aactcatgtt
     8341 tttatacaca gcttatcaac agaagttatt tgaatgaatc tatccacaag ttatccacag
     8401 gaagttgtgc acttatcaac agatttctgt tgataacttg tggataaggt tgaaatcagc
     8461 agacaattga cttttttatt tcctctatcg tgtctctaag atcaaaactc tcttcacact
     8521 tttttgttat ctgttcgtga gcatacataa ctgttgtatg gttttttcct ccgaaggctt
     8581 ctcctatttc aggaaaagac agattggaaa gttcacggca caaatacatt gctatctgtc
     8641 ttggaaaagc tatttccttg tttctcttct ttgacagtat gtcctcaaca gtgagattat
     8701 aataccttga aaccacatct ataataacct ttggggtaat ttcctttgtt tcagttttct
     8761 gtaagtgtct taatgcttct cttgccaggt tcaaatctat ttttcttcct tgtgaggagg
     8821 atattgtcag gtggttgatt ataccttcca gttctcttat gttgtctcct gtttggaatg
     8881 caatatgttc caaaatgttt ttctcgacaa taatattgtt ttcatcggct tttcttttca
     8941 aaatggccac ctttgtctca aagtcaggtg cctgtatgtc ggctatgagt ccccaggcaa
     9001 atcttgtacg caaacggtct tcaagtatgc ttatctcatt aatcggtctg tcactcgaca
     9061 caacaatctg tttgccatcg ctgtagagag agttgaatgt atagaaaaac tcttcctgtg
     9121 ttctttcacg tcttgatatg aactgaatat cgtcaatcag aagtacgtca atctttctat
     9181 acttgtttct aaactcatca ttcttttttt gttcgagcgc ctcaataaat tcgtttgtaa
     9241 atgtctcaga tgtgacatac agcacctttt ggttaggatc tttgtttaat atctcgtttc
     9301 caattgcatg cataagatgg gttttgccaa gcccggattg tccatatata aacaatggat
     9361 tatatgcctt gcctggactt gatgatatgg caacggcggc agcataagcc atacggttac
     9421 tgttgcctgt tataaaagaa tcaaaggtat atctgccatt cagatgagag ttgaaccttg
     9481 gtattatttg cttgttttcc tgctcctttt tcttgtattg atcgggcaaa taaaaatgca
     9541 atctatattc tttgcctgtg tgcaaagtaa ttgcatttct aatctgtgga taaaacttgt
     9601 tttctattgt gtttaacagg aatgcttctg gaacttcaaa ggctattatg tcctctgtta
     9661 tttccaatgg agttatactc ataatatacg cattgaacaa tgttggctgt atgtccgcac
     9721 ttaacgtatt caggcatttt ttccatatgc tttctaaaga atctgtcatt aatattcctc
     9781 catcttatgt atcaataatt cattttgagt caaaaaaata cttgtgtact tgtctattga
     9841 agcacacatt aaccttataa cgttctattt ctcattagta atctggtaat tttatcttat
     9901 gtataatcat gaactgttct gataagaacg tataaataat ataacaaata aacataccga
     9961 gtgcaacaga attttaaaag cagaaagaga ctgaatgctg tcataaatat gcatgatttc
    10021 aatgttttgt ggtaaagtgc attattaaat actgtatata gaaacaatca tgaaaataat
    10081 tcacaattaa atttgatacc catatttttg tctctcctac gttctgcgaa ggcaaacagt
    10141 tttattgaca gaattaaaaa ctttatatat aataacgctg atttggatgt atttatggat
    10201 ccaattagaa tacacgttgg aggtgatgta gaaatgctta gaacatatca gccaaagaaa
    10261 agaaagagaa gcaaacttca tggattcaga gcaagaatga aaacaagagg cggaagaaag
    10321 gtcctgaaag ctagaagaag aaagggaaga aaaattctgt ctgcgtaaaa tcaagggttt
    10381 tacgcagcgg tgtttggaga tagatgttta tgaaaaacat ggtttcgctt aaaacaaaca
    10441 gagagttctc aagagtcttt aaaaagggca agtacaaggc aggaaaacat attgtggtgt
    10501 atgcattgga aaacggtttt tcatacaaca gagtgggcat ctccatcagc aagaaagttg
    10561 gcattgctgt acaaagaaac agagtcaaaa gactaattcg cgaaagttac agattgagtg
    10621 aagacagcat attgacagga tacgacatag taattttggt aaagtcgacc gtccgggcag
    10681 caaaaacacc aaacaacaga ctaaaggccg tttctttgcc gtcgtttcat gatatagata
    10741 gcgaattcaa gaagcttggt atgaaactgg gaattttcaa aaaagaagaa agtgattaag
    10801 ctgaaaaggg tattaatttg gatcatcggc atatataaaa aatatttgtc acctttgaaa
    10861 agaccttgct gcaggtttta tcctacatgt tcatcgtatg caattgaagc aatagaaaaa
    10921 catggcgctg taaaaggact tgtgatgtca atttggagaa ttttaaggtg caatcctttt
    10981 aacaaaggag gatatgaccc tgtaaaataa gtaaaaggaa gtgtagcata acttgaattt
    11041 tataatcaag ccgttcggag tactaatgaa actgatatat gacacaattg cctttcaaag
    11101 ctatgggctt actttgatat atttcacaat attcgtaaga atattgcttt ttccccttaa
    11161 cttgaaacag caaaagtcat tgctcagaaa tcaggaactc caaccagaaa tagaagaact
    11221 aagacgactg tacaaaaatg acaaggaaaa attaaatgag gagcagatga agttatacca
    11281 aaagtataac gtaaatccca tggcaggttg cttgcctatg ttgattcaat tcccacttat
    11341 tatttcgctg tatcgcatag taacccagcc attgacctat atctccggtg tttccgcaag
    11401 tgctttaacc gccctaagga actttgtggc tggtgcccag aacatatcaa acagtaaaac
    11461 tgta
//
LOCUS       DAONVY010000009        10745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_4731_length_10745_cov_3.878297, whole genome shotgun sequence.
ACCESSION   DAONVY010000009 DAONVY010000000
VERSION     DAONVY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4731_length_10745_cov_3.878297"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLI11_00545"
     CDS             <1..799
                     /locus_tag="PLI11_00545"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ51387.1"
                     /translation="MSEEFAVSKVTPGELMSTGYSYLNCKMLHEFALILDKEEDAEYF
                     KEVMEKIKEAFLEKWFDKESCIVAAGSHASQAFPLWLGIIPEEYRQKVCVVLRNDLVQ
                     NNYQFTTGNLCTRYMLEVLTQYGYVDDAYEIMTKETYPSIGYMIQNEATTVWERFELK
                     KNPGMNSHNHPMYGSVYYWFYAYVLGIKPSEKAFDVVDIKPYFPSKLLSAHGEVETIK
                     GKISVRWANRYGNVHLYVTVPFGVTANVYLKDDEVVTVNSGCHYFKI"
     gene            complement(842..1948)
                     /locus_tag="PLI11_00550"
     CDS             complement(842..1948)
                     /locus_tag="PLI11_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPZ51388.1"
                     /translation="MSMMSDITKLKNSKTRLITAENVYGEKGKGGMAKLTTEPPEEIV
                     RIGQHWDGPSHAARELGEKWKVRPCITLLSGQETTIMDIDGPGRITHIWITVNPAFYR
                     DIILRMYWDHEEKPSAETPLGDFFLCGWKTPLNILSIPINVNPKGGLNSYFQMPFRKH
                     AKITVESRNPGNVGGFFYAITYEIEDFVAEEEAYFHAKFSRKNPLPYMEDFVILDNVK
                     GKGHYVGTQLSWQQNTQGWWGEGEVKMFIDGDTEYPTYCGTGTEDYFGGAWGFFGNFS
                     APFMGYQDLMTINENTRPTNSVGNRHSMYRFHILDPVRFEKDLKVTIQALGWRSEGRY
                     LPLQDDLSSVAYWYQMEPHNPFEPIGSRDELEII"
     gene            2171..2827
                     /locus_tag="PLI11_00555"
     CDS             2171..2827
                     /locus_tag="PLI11_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51389.1"
                     /translation="MSKKFIALLMVAVLSVAIFSISSFGAFDGYAMRFNTQEFLDKFN
                     LTNTEIELKDGYATATSTAHDPNIEYTFSETDVLDADKYKFAVMKYRTTYEQEGMIGE
                     FYFASDVSGYNHPATHAVFNLNSSGEWQRVVVDLSTFEAWTGNIKSLRIDYIEANNLP
                     AGAAMDIEYIAFFATKAEAEAFTDNFDDNPATSDQSVLFAFVTLLGFAAVVFKRKLAY
                     "
     gene            3018..4096
                     /gene="gcvT"
                     /locus_tag="PLI11_00560"
                     /pseudo
     CDS             3018..4096
                     /gene="gcvT"
                     /locus_tag="PLI11_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574062.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
     gene            4124..4498
                     /gene="gcvH"
                     /locus_tag="PLI11_00565"
     CDS             4124..4498
                     /gene="gcvH"
                     /locus_tag="PLI11_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPZ51390.1"
                     /translation="MKGERFYLKSHEWIEFIDEKTARVGISDFAQQELGDIVFVNLPE
                     VGDEVVAGEGFAEVESVKSISEVYSPVTGVVRSVNEKLQDSPELVNEDAYDAWFVEIG
                     DISAKEELLNEEEYNEFLESEN"
     gene            4502..5821
                     /gene="gcvPA"
                     /locus_tag="PLI11_00570"
     CDS             4502..5821
                     /gene="gcvPA"
                     /locus_tag="PLI11_00570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPZ51391.1"
                     /translation="MGTYVPSTEKERREMLASIGVCDMDDLFRTIPEDVMLKEPLDIP
                     KGMSELEVRKKMKGLANKNKVYSSIFRGCGSYNHYIPSCVGYIASKEEFLTTYTPYQA
                     EISQGILQAMFEYQTMICELTGMELSNASVYDGATAASEAILMCLERKKNKAVVSGAL
                     HPDVLETIKTYFSGSDVEICFVPCVDGKTDYDKMKECIDDSTACVVVGQISFYGQLED
                     IDKVKEVIGDVPLVMYVNPIALGMLKTPGEYGAQIAVGEGQSLGIPMSFGGPCLGFMA
                     CTKEYMRKIPGRIVGKTTDSRGRTAYVLTLQAREQHIRREKARSNICTNQANCALRAA
                     IYMSAMGKEGIKKAALLSMSKAHYLKDVLLATGLFELVYQGEFFNEFVTRSKDMDDRK
                     MLDFLSEKGILGGYPYEGNIIWCATEMNSKEEMDYLVSQIKEVAGCS"
     gene            5812..7242
                     /gene="gcvPB"
                     /locus_tag="PLI11_00575"
     CDS             5812..7242
                     /gene="gcvPB"
                     /locus_tag="PLI11_00575"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPZ51392.1"
                     /translation="MQLIFEKSVEGRKNDYLPECDVPVYEMDKKFMRESDLNLPEIAE
                     ADLSRHYTSLANRTFGVNKGFYPLGSCTMKYNPKINEEMAGLEGFANIHPLQPENTVQ
                     GCLEALHLAEEYLNKITGMDAISFQPAAGAHGEYTGLKLIKAYHDDRGDFKRDKIIVP
                     DSAHGTNPASAAMLGYKIINIKSSEDGFVDLAELEKAVGDDTAGFMLTNPNTLGLFDP
                     NILKITEIVRKAGGLNYYDGANLNAIMGRARPGDMGFDVVHLNLHKTFSTPHGGGGPG
                     AGPVGCKGFLKCFLPGPVVVKEDSIFKFFTPEKSIGRVKSFYGHFLVVVKALTYILQN
                     GCDGLKEVSESAVLNANYMMYKLKETYNIPYKGPCMHEFVISLEEEKAKYGFTALDIA
                     KALVDKGMHPPTMYFPLVVHEALMVEPTETESKETLDKAIEDFIAVYEQAKQDVEKVK
                     NTPVTTPVGRPDEATAARNPIIKYEG"
     gene            7394..8434
                     /locus_tag="PLI11_00580"
     CDS             7394..8434
                     /locus_tag="PLI11_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPZ51393.1"
                     /translation="MQRKRIFVIAILLIQILATEVIAYNYKEQLDSTKSEIYERIEEI
                     VKTGEEFADLTDIYDKGYYSKEELDVIVKKIGVDTQAAIDAFLKDHPEIYWLKFGEGG
                     VGASYNIETIGSFNRVDDVFFQIVTDEKYEGKLEEIDKALWERVNEFEVHGETRYEKV
                     KSIHDSLIEHVEYDGVFLYSHEAVGALVYGKAVCEGYAKAFKLICDREKIPNVLVIGT
                     GVTPDGREEDHMWNYVQMEDGNWYAVDVTWDDQDKLVYDFFLVGSKSNSFGETHIPNG
                     DFSGINKYVFTYPPLEENAYGLASPEPEEDMPRESIISTENKESNHIGPYIALVFGVC
                     LLGIVVVIYKRK"
     gene            8447..10645
                     /locus_tag="PLI11_00585"
     CDS             8447..10645
                     /locus_tag="PLI11_00585"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51394.1"
                     /translation="MEGKWIFTEAKRTEEPMMVCFRKEVLVDRVCDRCTIQISADSRY
                     FLYINSDMVDCGPAKMPAGLRCYDELDITPYIKQGNNEIFVKVLCYPKDSLKALGFSS
                     GPISVITEGVGGLLIHELDTNYGLSTDESWMCAKIDGYGFVKNKFAGYVGFSEHIDYE
                     RVQASLDNNMKNAVVVMDSANSNPYGLASFWQLVKRPIPQMYMEKKDTFKLMRQYDNT
                     YEYDAQVYVFAFPAIKVSGKQGGFAKLTYAESYGRFEETGYVKGVRNNPDRQEIIGEV
                     DYITLQEGETIYIPFYPRAFRFVKVEVSEDVEVEEFFYYETGYPLDVQAEFKADDEVY
                     EKIWDVSLRTLKLCMYETYVDCPYYEQMQYMMDTYLEAIYTMSVSDDSRLVKKAINDF
                     AYSQLPNGLLPCNAPSSFVQVIPGFAIYWLLLLDAYFMYEDDVEFVKGHLGTVDRIID
                     FFTRHINEDGLLGDTGFWQFFDWVDGWERGVPVQKGVNILYNMLFVLGLRTAAKLNLS
                     VGRLSTHNEYLALANSLSYQIIKHSFDKETGLFKNSPNDEKPVSVHAQVFAVVAQVVM
                     CEDNKRALMRTTVENAGKLKPMSYAMRFFLCRALEETDMYDEVYKTFRIWDAYKELLE
                     LDLTTWPEDFVTQRSDCHGWSALPLSEFVRCYLGVRPIEYGFKKVGIYIYDSPFEKYS
                     GTAVAKGKEIFVDIDNVTGNVALRIPKGLDYELKDFRKNVQSPKYLISTY"
BASE COUNT         3271 a         1886 c         2558 g         3030 t
ORIGIN      
        1 catgagcgag gagtttgcag tgtcaaaagt aactcccgga gaacttatgt ccaccggcta
       61 ttcatacctt aactgcaaaa tgctgcatga gtttgcgctg attcttgaca aggaagagga
      121 tgcagagtat ttcaaggaag tcatggagaa gataaaagag gcgtttcttg agaagtggtt
      181 tgacaaggaa tcgtgtattg tggcagcagg ctcgcatgca agtcaggctt ttcctctgtg
      241 gctgggcata attccagagg aatatagaca aaaggtgtgc gtcgtgctga gaaacgacct
      301 ggttcaaaac aactaccagt ttacaacagg aaacttgtgt acaagataca tgctggaagt
      361 acttactcaa tacggttatg tggatgatgc gtatgagatt atgacaaagg agacgtatcc
      421 ttccataggc tatatgatac agaatgaggc tacaaccgtt tgggagaggt ttgagcttaa
      481 gaagaatccg gggatgaact cccacaacca tccgatgtac ggctctgttt attactggtt
      541 ctatgcttat gttctgggaa tcaaaccttc cgagaaggct tttgatgttg tggacataaa
      601 gccatatttt ccatcaaagc tgctttctgc ccatggggaa gttgagacaa tcaaaggaaa
      661 aatttctgta agatgggcaa acagatatgg caacgtacat ctttatgtga cagttccttt
      721 tggagttact gcaaatgttt acctcaagga tgacgaagtt gtaacggtaa actccggctg
      781 tcattatttc aaaatctgaa caaagcaata aattagctgg cagaactgcc agctaattta
      841 tttatattat ttcaagttca tctcttgaac caatgggctc gaatggattg tgaggctcca
      901 tctgatacca gtatgccact gatgaaaggt cgtcctgaag cggcaagtat cttccctctg
      961 atctccagcc taatgcctgt atcgtaacct tcagatcctt ttcaaacctg acagggtcaa
     1021 gtatatggaa tctgtacatc gagtgcctgt ttcccacgct gtttgtaggt cttgtgtttt
     1081 catttatggt catcaaatcc tgatagccca tgaaaggagc tgagaagttt ccaaagaagc
     1141 cccaggctcc gccaaagtag tcttcggttc ccgttccgca atatgtggga tattcagtat
     1201 cgccatctat aaacattttg acttctcctt caccccacca gccttgtgtg ttctgctgcc
     1261 atgatagttg tgttcccaca tagtgaccct tgcctttaac attgtcaaga atcacgaagt
     1321 cttccatgta tggcagtgga ttcttccttg agaactttgc gtgaaagtat gcctcttctt
     1381 cagcaacaaa atcctctatc tcatatgtaa ttgcatagaa gaaaccgcca acattgccag
     1441 ggtttctgct ctcgactgta atttttgcat gttttctgaa aggcatctgg aagtacgaat
     1501 tcaaaccacc tttaggattt acattaatcg gaattgaaag tatgttgagt ggagtcttcc
     1561 atccgcataa gaagaagtct cccaggggag tttccgcgct tggtttttcc tcgtggtccc
     1621 aatacatcct caggataata tcacggtaaa aagcaggatt tacagtaatc cagatatggg
     1681 ttattcggcc tggtccgtca atatccatta tcgttgtttc ctgcccggat aacagggtaa
     1741 tgcaagggcg taccttccat ttctcgccca gctctcttgc tgcatgactt ggtccgtccc
     1801 aatgttgacc aattctaact atctcctctg ggggttcggt tgtcaatttt gccattccgc
     1861 cttttccttt ttcaccatac acattctctg cagtaataag gcgggttttg ctgtttttaa
     1921 gtttggtaat atcgctcatc atgctcatat atgatcgccc tccataatat taagtacaat
     1981 atttaagagt atagtaccat atttgtgtaa ggattataag gtgtgaattt tgcatttatt
     2041 aaaaggatta tagcaattat atattttctt ggtgttttac acccttttgt tgaatattta
     2101 attaaagata ttgagatatt ttcataagta ttgtataata tgtaacgaat aaatctaagg
     2161 gggtaacatt atgtccaaga agtttatagc gttgttaatg gttgctgtgc ttagtgtagc
     2221 cattttcagt attagttctt ttggtgcatt tgatggatat gcaatgcgct tcaatactca
     2281 ggagtttctt gacaagttca atcttacaaa taccgaaata gaattgaaag atggatacgc
     2341 tactgctaca tcaacagcac acgacccaaa tattgaatat acattcagtg agactgatgt
     2401 acttgatgct gacaaataca agtttgcggt tatgaagtac agaactacat acgaacagga
     2461 aggaatgatt ggtgaattct attttgcttc ggatgtatcc ggttacaatc atcctgccac
     2521 ccatgcagta ttcaatctga attccagtgg cgagtggcag agggttgtag ttgatctcag
     2581 cacctttgaa gcatggactg gaaatatcaa gtctcttaga attgactaca tcgaggctaa
     2641 taatcttcct gcaggtgctg ctatggatat tgagtacata gccttttttg caactaaggc
     2701 agaagcagag gctttcacag acaattttga tgacaaccca gctacatctg atcaaagtgt
     2761 tctgtttgca tttgtaactc ttcttggttt tgcggctgtt gtattcaaga gaaagctggc
     2821 ttattaaaat ctttgcataa aaaaagggcg caagcccttt ttttgtgcct taattaaatt
     2881 ttttaatatg gtttttattc tattttattt ggcataatta aaatgtattg acttgattgt
     2941 attctgtatt taattgacaa tacttgtgta taaacatata atcaatctgt ataaataatc
     3001 cgaaaggagc actgatcttg gaaaagaaaa caccgatata tgaacgtcac gtggagttga
     3061 aggggaggat ggtacccttt gcaggatatc tgcttcctgt acaatacgaa accggcgtta
     3121 taaccgagca taatgcggta agaaacaagg ctggcttgtt tgatgtttcc cacatgggac
     3181 aaatagtttt acaggggccg gatgctcttg aaaatataaa tatgcttttg acaaatgatt
     3241 ttaccaacat gtccgatgga agagcaaaat acagccctat gtgcaatcca gagggtggta
     3301 ccatagatga cttgatagtc ttcagacaaa gcaaggataa atatctgttg ggagttaatg
     3361 cggccaacag ggacaaagat tttgagtgga taaaaagcaa tgtaaagggt aatgttaagt
     3421 actcgacata tccgatgaca ttgcacaagt tgcccttcaa ggtcccaatt cttatgatat
     3481 tctcaggaaa ttgacgaata atattcctga aaaatattac tccttccttg aagatgtgga
     3541 tgttaatggt ataatttgtt ttgtttcaaa aaccggatac acaggcgagc tgggttatga
     3601 gataagctgc gacaagacag atggtgtcaa actgtggaac atgctactgg acgcaggtga
     3661 agagtttggt ctcattcctt gtggtttggg tgcaagggac actttaaggc ttgaagcggg
     3721 catgccattg tacggtcatg aattgtccga ggaaatatca ccccttgaag cttcactaaa
     3781 ctttacggtc aaaatggaaa aggaagactt cataggcaag gaaggaataa ggaagagggg
     3841 cacaagcagg aggcgggttg gtctcaagat cactggaaga ggcatagcaa gagaagactg
     3901 tgatgtgttt gtggatggac aaaaggttgg tttgaccaca tcgggaaccc atagccctac
     3961 tcttggatat cctatcgcca tggcattgat ggataaagac tacactgata taggaattga
     4021 agtggttgtt aacgtaaggg gcagggaaat tcctgcagta atttgtgatt taccctttta
     4081 tactcgagta aaataaattt agattaatca tggaggaatt gatatgaaag gcgaaagatt
     4141 ctatttgaaa tcacatgaat ggattgagtt tattgatgaa aagacagcaa gggttggcat
     4201 aagcgatttt gcacagcagg aactgggaga tattgtgttt gtaaatcttc ctgaggttgg
     4261 cgacgaagtt gttgcaggcg aaggttttgc agaggttgag tctgtcaagt caatatctga
     4321 agtatacagc cctgttacag gagttgttag aagtgtaaat gaaaaactcc aagacagccc
     4381 cgagctagtt aatgaagatg catatgatgc atggtttgtt gagattggag atatttcagc
     4441 caaagaggaa ttgttgaacg aagaagaata caacgaattt ttggaaagcg agaattgata
     4501 aatgggtacc tatgttccta gcaccgaaaa agaaagaagg gaaatgcttg catccatagg
     4561 cgtttgcgat atggatgacc ttttcaggac tattcctgaa gatgtcatgc tgaaagagcc
     4621 tttggatatt ccaaagggaa tgagtgagtt ggaagtccgt aaaaaaatga aaggacttgc
     4681 caacaagaac aaggtgtatt catccatttt cagaggctgc ggctcataca accactacat
     4741 ccccagctgt gtcggataca ttgcctcaaa ggaagagttt cttacaacct atacacctta
     4801 ccaggcagaa atttcccagg gcatactcca ggcaatgttt gagtatcaga cgatgatttg
     4861 cgagcttaca ggcatggagc tttcaaatgc ttcagtctat gatggcgcca ccgctgccag
     4921 cgaagcaatc ttaatgtgtc ttgaaaggaa aaagaacaag gctgttgtat caggagcact
     4981 gcatcccgat gttttggaaa ccatcaaaac atatttttca ggctccgatg tggaaatctg
     5041 ttttgtacca tgtgtggatg gtaaaacgga ttatgacaag atgaaagagt gcatcgatga
     5101 cagtactgcc tgcgtggtag taggccagat aagcttttat ggacaactgg aggacattga
     5161 caaggtgaaa gaagttatcg gcgatgttcc tcttgtaatg tacgtaaatc ccattgccct
     5221 tggcatgctt aaaacacctg gggagtacgg tgcgcaaata gcggtaggag aaggtcagag
     5281 ccttggcatt cccatgtcct ttggcggtcc atgccttggc tttatggcct gcacaaagga
     5341 gtatatgaga aagataccgg gaagaattgt aggaaagaca acggattcaa gaggacggac
     5401 tgcttatgtt ttaaccttgc aggcaagaga gcagcatata agaagggaaa aggcaaggag
     5461 caacatatgt acaaaccagg caaactgtgc tcttagagct gccatataca tgagtgcaat
     5521 gggcaaagaa ggtataaaga aggcggcttt actttccatg tcaaaggcac attatctaaa
     5581 agatgtactt cttgcaaccg gattgtttga acttgtgtac cagggcgagt tctttaatga
     5641 gtttgtgaca agaagcaaag atatggatga caggaagatg cttgattttc tttctgaaaa
     5701 gggtattctt ggcggatatc catatgaagg caacatcata tggtgtgcaa ccgagatgaa
     5761 tagtaaagaa gagatggatt atcttgtgtc gcagataaag gaggtggcag gatgcagctg
     5821 atatttgaaa aaagtgttga aggcagaaag aacgactacc tgccagaatg cgacgtgcca
     5881 gtatatgaaa tggacaaaaa gtttatgaga gagagcgatt tgaatcttcc tgagattgcc
     5941 gaagcagatt tgtccaggca ctacacatcc cttgcaaaca gaacttttgg cgtaaacaaa
     6001 gggttttatc ctttgggttc ctgcaccatg aaatataatc cgaagattaa tgaagagatg
     6061 gcaggccttg agggttttgc aaacattcat cctctccagc cggagaatac ggtgcaagga
     6121 tgccttgagg ctttgcatct tgctgaggag tatctgaata aaattaccgg catggatgct
     6181 ataagtttcc aacccgcagc cggcgctcat ggcgaatata caggattaaa actcattaaa
     6241 gcataccatg acgatagagg ggattttaaa agggacaaga taattgttcc ggattccgct
     6301 catggaacga atcctgcaag cgcagccatg ttggggtaca aaataataaa tattaagtcc
     6361 agtgaggatg gatttgttga tcttgcagag ctggaaaagg cagtagggga tgacactgct
     6421 ggtttcatgc tcacaaatcc aaatactctt ggtctttttg acccaaatat attgaaaatt
     6481 acggagattg taaggaaagc gggaggcctc aactattacg acggtgcaaa ccttaatgca
     6541 attatgggta gggcaagacc cggcgatatg ggatttgatg ttgtgcacct taacttgcac
     6601 aagaccttct caacacccca cggcggcgga ggtcctggtg cgggtcctgt tggatgtaaa
     6661 gggttcttga aatgcttcct gccaggtcct gtggttgtaa aagaagatag tatttttaaa
     6721 ttctttacac ctgaaaaaag cattggccgt gtaaagagtt tctacggaca tttcctggtg
     6781 gttgtaaagg ctttaacata tatcctgcag aatggctgcg atggtctgaa ggaagtttca
     6841 gaaagtgcag ttttgaatgc caactacatg atgtataaac taaaagagac ttacaacatt
     6901 ccatacaagg gtccgtgcat gcatgagttt gtaatatcat tggaggagga aaaggcaaag
     6961 tacggtttta ctgctcttga tattgcaaaa gctttagtag acaaaggcat gcatccacct
     7021 acgatgtatt tcccgcttgt ggtgcatgaa gctctgatgg tggaacctac tgaaaccgaa
     7081 tccaaggaaa cccttgacaa ggcaattgaa gacttcatag cggtatacga gcaggcaaaa
     7141 caggatgtgg agaaggttaa gaataccccg gttacaacac cggtgggcag gccggacgag
     7201 gcaaccgctg cacgaaaccc aattattaaa tatgaaggat aaattaaggg gggcttgaaa
     7261 cacagctccc cttgaattat gctcaaaaac cttaaattaa ctatggttta tgggcaataa
     7321 tcttaattaa atacattgaa ataatgggtt ttgacatata tactaatctg gtataaatct
     7381 attggtggta caaatgcaaa gaaaaaggat attcgttata gctatattac tcatacagat
     7441 actcgcaaca gaagtcatag cgtacaatta caaggaacag cttgattcaa caaaatcgga
     7501 gatttacgaa agaatagagg agattgtaaa gacgggggaa gagtttgccg atcttaccga
     7561 tatatatgac aaggggtatt attcaaaaga agagcttgat gtgattgtta agaaaatagg
     7621 ggtggacaca caagctgcaa tagatgcttt tttaaaagac catcctgaaa tatattggct
     7681 taaatttgga gaaggaggag tgggtgccag ttacaatatt gaaaccatag ggtcttttaa
     7741 tagagtagat gacgtgtttt ttcaaattgt taccgatgaa aagtatgaag gcaagcttga
     7801 ggagatcgac aaggcacttt gggaaagagt aaatgaattt gaagtgcatg gggaaactcg
     7861 gtatgagaaa gtaaagagca tacatgatag tttgattgaa catgtggaat acgatggcgt
     7921 ttttttatac tcccatgagg ctgtaggtgc tttggtttat gggaaagcgg tttgcgaagg
     7981 atacgcaaaa gctttcaagc ttatttgcga cagggaaaaa atccccaatg ttttggttat
     8041 agggacgggg gtaacgccgg acggaagaga agaggaccac atgtggaatt acgtacaaat
     8101 ggaagacggc aattggtatg cagtggatgt tacctgggat gatcaggata agttagttta
     8161 cgacttcttt cttgtaggca gcaaatcaaa ttcttttggt gaaacccata tcccaaacgg
     8221 ggacttctcc ggcataaaca aatatgtctt tacttatccg cctttggagg aaaatgcata
     8281 tggattggca agccccgagc ctgaagaaga tatgccaaga gaaagcataa tcagtacgga
     8341 gaataaggaa tcaaaccaca ttggaccata tatagccctg gtctttggtg tatgtttatt
     8401 aggaattgtg gttgttatat ataaaagaaa ataaaggagg catttcatgg aaggaaagtg
     8461 gatttttaca gaagccaaaa gaacggaaga accgatgatg gtgtgtttta ggaaagaagt
     8521 gcttgtagac agggtatgcg atagatgcac catacaaata tctgcagatt caagatactt
     8581 tttgtatata aacagcgaca tggttgattg cggacctgcg aagatgcctg caggtttaag
     8641 atgctatgat gagcttgaca taacaccata tataaaacaa ggaaataacg agatttttgt
     8701 caaggtactg tgctatccaa aggattcttt gaaggctttg ggtttttcct caggccctat
     8761 atcagtaatc actgaagggg tgggaggact tctcatacat gaattggata cgaactatgg
     8821 cctttcaacg gatgaaagct ggatgtgtgc aaaaatagac ggatatggat ttgtcaaaaa
     8881 taaatttgcc ggctatgtcg gattttcaga gcatattgat tatgaaaggg tacaggcaag
     8941 ccttgataat aatatgaaga atgctgtagt cgtcatggac agtgccaatt cgaaccctta
     9001 cggtcttgca tccttctggc agcttgtcaa aagacccatt cctcaaatgt acatggagaa
     9061 gaaggataca ttcaagctga tgaggcagta tgacaacact tatgaatatg atgcgcaggt
     9121 gtatgtattt gcatttccag cgataaaagt ttccggaaag caaggtggct ttgccaaact
     9181 tacgtacgct gaaagctatg gtaggtttga agaaaccgga tatgtaaaag gagtaaggaa
     9241 caatccagac agacaggaga ttattggaga ggtagattat atcaccttgc aggaaggaga
     9301 aacaatttac atcccctttt atccaagagc cttcaggttt gtaaaagttg aagtgtcgga
     9361 agatgttgaa gtagaagaat tcttctacta tgaaaccggc tatcctttgg atgtccaggc
     9421 tgaattcaag gcggatgatg aagtgtatga aaagatatgg gatgtaagct tgaggacgct
     9481 aaagctctgc atgtatgaga cttatgtgga ttgtccgtat tacgagcaga tgcagtacat
     9541 gatggatacc tatcttgaag ccatatacac catgtccgta tcggatgatt caaggcttgt
     9601 gaaaaaggca ataaatgatt ttgcgtattc gcaattgccc aatggccttc ttccatgcaa
     9661 tgccccttca agtttcgtac aggttatccc aggctttgca atctactggc ttcttttgct
     9721 cgacgcatac tttatgtatg aggatgatgt ggagtttgta aaagggcatc ttggtaccgt
     9781 agacaggatt attgatttct ttacaaggca cataaacgaa gatggacttc ttggcgatac
     9841 aggattttgg caattcttcg actgggttga cggctgggag agaggagttc ctgttcagaa
     9901 gggtgttaat attctgtaca acatgctgtt tgtgttaggt ttgagaactg ccgccaaact
     9961 gaatctgtct gtaggcagat tgtcaaccca taacgagtac ttggcattgg caaattcctt
    10021 gtcctatcag attatcaagc attcttttga caaggaaacc ggccttttca agaattctcc
    10081 taatgatgaa aagcctgtaa gcgttcatgc acaggtgttt gcagtggttg cgcaagttgt
    10141 catgtgtgag gataacaaaa gagcgttaat gagaaccact gtggaaaatg cgggaaaact
    10201 aaaacccatg tcctatgcta tgaggttctt tttatgcagg gctttggaag agacagacat
    10261 gtacgacgag gtatataaaa ccttcaggat ttgggatgcg tataaagagc ttcttgaact
    10321 tgaccttacc acatggccgg aggattttgt cactcaaaga agcgactgcc atggctggag
    10381 cgctttgcct ctttctgaat ttgtccgctg ctatttgggt gtaaggccaa ttgagtatgg
    10441 tttcaaaaag gttggcatat atatttacga cagtccgttt gaaaaatact ccggtactgc
    10501 agtagctaaa gggaaagaga tttttgtcga tatagataat gtgacaggaa atgttgcttt
    10561 aagaattcct aaaggtttgg attacgaact gaaggacttc aggaaaaacg tccagtcgcc
    10621 aaagtatttg atttccacat attaatgtgt taaaataaat agtattgtta caagtagaga
    10681 actttgcttg gttctctact ttattgcaat aatgaggtgg taaatatgac caaaacagca
    10741 gttga
//
LOCUS       DAONVY010000010        10743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_4734_length_10743_cov_4.035647, whole genome shotgun sequence.
ACCESSION   DAONVY010000010 DAONVY010000000
VERSION     DAONVY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4734_length_10743_cov_4.035647"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLI11_00590"
     CDS             <1..524
                     /locus_tag="PLI11_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014568276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPZ51395.1"
                     /translation="VRINIIHGAVGAVNDSDVNLAEVSNAVIIGFNVRPAPNVADHAE
                     SVGVDIRLYRVIYDAIEDIETAMKGMLDPEYKEVVQGMVEVRQIFKVSGIGTIAGGYV
                     IQGKITRNSGVRIVREGIVIHEGSLASLKRFKDDVREVVQGYECGLSIEKYNDIKVGD
                     IVESFVMEEIKRN"
     gene            527..901
                     /gene="rbfA"
                     /locus_tag="PLI11_00595"
     CDS             527..901
                     /gene="rbfA"
                     /locus_tag="PLI11_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPZ51396.1"
                     /translation="MDGRTVRIANEMKKVISDILMYDLKDPRIPQMTSVTKVDLTKDL
                     KHAKVYVSIYGSEADKASCMLALKKSAGYIRREIGRRMIIRALPELDFKLDESIEYGA
                     YMSELIDKVVPKEERDDGGSDE"
     gene            879..1871
                     /locus_tag="PLI11_00600"
     CDS             879..1871
                     /locus_tag="PLI11_00600"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPZ51397.1"
                     /translation="MTEAAMNSWSKAVEVINKARSISIFTHYDADGDALGSAFALAIE
                     LKRLGKETCVFLDEEMQKKLVFLSSKGIDTVVYEVGMETRRFDLSIAVDSGDMKRLGK
                     RAELFKSAAVTMKIDHHISKDDFGDYNFVDTKWAATCVAIWELLTELVGEISDEAAIR
                     IYTGIYTDTGGFKHSNTDERVHLITAKILSKLGDLSYISRIFQEKSKGMIKLYNIAVS
                     KMEFFFNERVVYAYLAKEDFEKAGAIMEDSEGLATYLREIEGVDTSVFIRQGDKEDEL
                     KASLRSNEIVDVAEIAASFNGGGHKRAAGFTYYGDEQSMKKMLLERLGRTLNDT"
     gene            1861..2727
                     /gene="truB"
                     /locus_tag="PLI11_00605"
     CDS             1861..2727
                     /gene="truB"
                     /locus_tag="PLI11_00605"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPZ51398.1"
                     /translation="MILNVYKPKGISSFQCVSEVRRRTKAKKAGHAGTLDPNAEGVLP
                     VMLDKSTRVNEFFLDFDKGYECEILFGKETDTCDIWGEVVREEEYSHITDEALLEVIK
                     SFLGHQKQIPPVYSAIKVDGVPMYKLARKGKAVEPKARDIEIYSIDILAFERNIARIH
                     VNCSKGTYIRSLCRDIGVALNSAACMYSLIRTHYGMFDYKDAVRLENINENYMEYAHP
                     VDLVLKHFPSVKINDKQKVDYLNGRSVLINGIFEEEGIRVYHKGELLALARNEKTKDG
                     NTVLKPWKYFGE"
     gene            2738..3700
                     /locus_tag="PLI11_00610"
     CDS             2738..3700
                     /locus_tag="PLI11_00610"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPZ51399.1"
                     /translation="MEVLYRLSNTRIPENSYGLGLGNFDGIHLGHARIIDVLSEECGK
                     LGIPSVIYTFRRHPENILKKDKKTPIILSNEQKASILEKHNIDILYFEEFTEEFSKIK
                     PYDFVKNILVGEFNAKLLVVGYDYTFGIKGLGKANDLIELGKEFNFKTVIVPPVEVEG
                     TAVSSTFLRKLISLGDMEKYTSMTNRQYSIPGEVTVGRKVGSKIGFPTANILPREGYA
                     LPSAGVYATKTKLGDVMYESITNIGNNPTFGCKLTTIETHIFDYEDKLYGQNIEVFFY
                     KKLRDEMVFNSVDELRARIATDIDDAKQYFKMTSRRNGVNGENS"
     gene            3684..4970
                     /locus_tag="PLI11_00615"
     CDS             3684..4970
                     /locus_tag="PLI11_00615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPZ51400.1"
                     /translation="MEKIAEINGIDVYYIRSSKFKTGSINITFCDNLSKERAYKNALI
                     PSILARGCKTHPTQRDISIKLMSLYGAGFSTNVEKKGEIQLIQFVADFVEEVYAKNYK
                     NLVRELVELLFNIICNPVVEGSGFKKEYFQQERVNNNNIIRSLINNKQSYAIVRCQEL
                     MCKDEPFGIKELGSVEDGEHLTETELYKYYSEFFLKKLPVKIFFCGRNKPDDLMEVLN
                     SFKDEFAGEKIKLNLGYREFGTIERNDVVEKMDVTQGKLTIGFRTNVDPDSDDFYALC
                     VANGIFGAGVHSKLFQNVREKNSLAYYAASRIERYKGLMVAYSGIEIANKEKAEELIL
                     KQLQEVKDGNVTENEYESTIRMFKNAFNSFKDTQFAIMDFFIGQTFLNTEILHLDEFI
                     EKVSNVTVEDAVRCAQKIKLDTVYFLTSNNMEVIES"
     assembly_gap    5063..5072
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5073..6241
                     /locus_tag="PLI11_00620"
     CDS             <5073..6241
                     /locus_tag="PLI11_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPZ51401.1"
                     /translation="AIFAVRFGSVDLEFVAPMDNHVTKVPDGVAHFLEHKLFEQEDGN
                     MLDKFVSLGASPNAATSFNWTYYYFTCTDRFEECFGNLLYFVLNPYITEESVEKEKGI
                     ISQEINMYLDAPDFIVSMELLRLMYHNNPIRNDIAGSVESISKITADILLKCHETFYH
                     PSNMVVTVVGDVDPKEIQDVVDRHIPKTGREGDIKRIEKEEPLALKGTESILTMDVSL
                     PLFNIGFKDDPVKLFGSERVRREIAGSILKEMMFGKVSTFYETLYNEGVISNDFYADY
                     ETGANYALFSLGGESQKPQRVREYLEQYIEEIRKNGFDKKYFEMVKNALSGRVLRRFN
                     SPESMGRMFASSYLIGVDAFDYFTAYGTINLDESYHIFEEVFLKPMAVSIVNPK"
     gene            6257..7243
                     /gene="lgt"
                     /locus_tag="PLI11_00625"
     CDS             6257..7243
                     /gene="lgt"
                     /locus_tag="PLI11_00625"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPZ51402.1"
                     /translation="MDNIIEFPKLGLEFHINDVAFDAIGNFEIKWYGIIICFGFLLSI
                     ILAMRVCENFDISKDDLLDYLLFAMPAAIVGARLFYVVFAFDEYKDNLKEIFNIRQGG
                     LAIYGGVIFAVITVLIVSKVKKKSVLNVLDFAAPYIMLGQAIGRWGNFTNQEAFGGAT
                     NLPWGMTGNLIKERLEYYGLSRQQLVHPTFLYESLICFVGFAFMMIYRKRWQKEKGEI
                     LCLYMIIYGIARAIIESLRVDSLMLGNVRVSRLISIVIFVLGITLLIDIRRRHKLKMI
                     ETSEEESSLRKIVEVIETSDEEDTMQEAEALEAARQAELMAYELQDEYTERE"
     gene            7257..8396
                     /locus_tag="PLI11_00630"
     CDS             7257..8396
                     /locus_tag="PLI11_00630"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPZ51403.1"
                     /translation="MRFSGIERSQRIDYFRKFDYLLMGVVLIITAIGLVFLHSAMYDM
                     YADNGASAMTVQIAGVILGIIVALFFCFFDYTNFRNVCFPFYVFNLFVMLLVFTPIGI
                     ENYGSRSWIDLYVTTYQPSELMKIAMIILIAKYLERINENGMTLDSAIIILAGFFIPL
                     GLVLLQKDLGMALVFIFIFLVMIFVGNVKLWQIGIIIGGGLAAVPFVWKFYLNGVKRD
                     RFLSFLNPDKYQDYSLQLRRAMTAIGSGQLFGKGYMQGPLNRGRKIPVKMSDMIFAVI
                     CEEGGFIAGFAIVVLMTIMLLRMLQVSHRARDVFGSCIAAGIFAMFAFNIFENIGMNI
                     GIMPITGLPLPFVSKGGSAMVTNFFSIGLLLSISLRNKKGMFIDD"
     gene            8618..9049
                     /gene="mraZ"
                     /locus_tag="PLI11_00635"
     CDS             8618..9049
                     /gene="mraZ"
                     /locus_tag="PLI11_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPZ51404.1"
                     /translation="MFTGATFNTIDAKGRVVIPQKFRDTLSDTGFVITRGMDGCVYIY
                     PKPRFQELTEKLMQMQSTGVETRKLARFLLAFAEEGDFDAQGRICVSQPLREYAGLKK
                     EILTTGILDRIEIWDRERWNAYSEENLEGPDFNEAVIGFDF"
     gene            9063..9992
                     /gene="rsmH"
                     /locus_tag="PLI11_00640"
     CDS             9063..9992
                     /gene="rsmH"
                     /locus_tag="PLI11_00640"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPZ51405.1"
                     /translation="MDFQHTPVLLEETIRYLDIRQDGVYVDGTVGGAGHSKKILELLS
                     EKGLLICLDQDEKAIEAAGKKLEGAKNVILLKTNFVNLISVCNSLGVSKVDGILFDLG
                     VSSHQFDTPERGFSYRFDSKLDMRMDADSELTAYDVVNGYEEAELRRIIKEYGEERFA
                     SRITGKIVERRKIKPIETTFELVEVIKEAIPKKQRMDGHPAKQTFQAIRIEVNKELEV
                     LEKAIEDAIEMLAPFGRLCIITFHSLEDRIVKTKFNNFENPCTCPPDFPICVCGKKPK
                     GKAVTKKPILPSQEEMERNPRAKSAKLRVFQSN"
     gene            10243..>10743
                     /locus_tag="PLI11_00645"
     CDS             10243..>10743
                     /locus_tag="PLI11_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51406.1"
                     /translation="MNSAGARELHTEDYDYDNAYDYGYDFDYDYEERIEQSPEFAVLR
                     ELKIKRNNAKVKANLVIKVSIIIILGLIIAFRYASITELGYKVNAMQNQLDEIKAENS
                     RLETNIARQMNLAEISEIAINELGMQKPQSYQIVRINVVPTDQTEIHEMKFTKDENNT
                     PWYKNVL"
BASE COUNT         3488 a         1719 c         2568 g         2958 t
ORIGIN      
        1 aggtaagaat aaacattatc catggagctg ttggtgctgt taacgactcc gacgttaatt
       61 tggcggaagt atcaaacgcc gtaattattg gttttaacgt aaggccagct ccaaatgtgg
      121 cagaccatgc tgaatccgtt ggtgttgata tccgtctcta cagggttata tacgacgcta
      181 ttgaggatat cgagactgca atgaagggta tgcttgatcc cgagtacaag gaagtagtac
      241 agggaatggt tgaagtaagg caaatcttca aggtatccgg cattggtaca atagcaggcg
      301 gatatgtaat ccagggcaag attacaagga actcaggtgt taggattgta agggaaggca
      361 ttgtaataca tgaaggctct cttgcatcac tgaagagatt caaggatgac gttcgagagg
      421 ttgttcaagg ttacgaatgc ggtctttcca tagagaagta caatgatata aaagttggcg
      481 atattgtaga aagctttgta atggaagaga taaagaggaa ttaaaaatgg acggaagaac
      541 tgttaggatt gcaaatgaaa tgaaaaaggt catttccgat attcttatgt atgatctcaa
      601 agatcctaga atacctcaaa tgacctccgt gacgaaagtg gatcttacaa aagatctaaa
      661 gcatgcaaag gtttatgtaa gcatatacgg cagcgaggca gacaaggctt cctgcatgct
      721 tgcattgaaa aaaagcgccg ggtatataag aagagagata ggaaggcgta tgataatacg
      781 tgctcttcca gagcttgact tcaagctgga cgagtctatt gagtacggag catacatgtc
      841 cgaactcata gacaaggttg ttccaaagga agagagggat gacggaggca gcgatgaata
      901 gctggtccaa agctgttgag gttataaaca aagcccgaag catttccata tttacgcatt
      961 atgatgcgga tggcgatgct ttgggttctg cttttgcatt ggcaatagag ctaaaaagac
     1021 tgggcaagga gacttgcgtg tttttggatg aggagatgca gaaaaagctg gtgttccttt
     1081 cctccaaagg cattgacact gttgtgtatg aagtgggcat ggaaacacgc aggtttgact
     1141 tgagtattgc tgttgacagt ggcgatatga agaggcttgg aaaaagagct gaattgttta
     1201 aaagtgctgc tgttaccatg aaaattgacc atcacatctc caaggatgac tttggagatt
     1261 acaattttgt agatacaaag tgggctgcca catgtgtcgc catatgggag cttttgactg
     1321 aactcgttgg tgagatatcg gatgaggcag caattagaat ctatacaggg atttataccg
     1381 acacgggcgg attcaagcat tcaaacacgg atgaaagagt gcatttaata acagcaaaga
     1441 ttctatccaa gctgggagat ctgtcttaca taagcaggat tttccaggag aaatcaaaag
     1501 gaatgataaa actgtataat attgcggtct cgaagatgga gttctttttt aatgaaaggg
     1561 ttgtttatgc ataccttgca aaagaggatt ttgaaaaggc gggagccatc atggaagaca
     1621 gtgaagggct tgccacgtat ttaagagaga tagaaggcgt ggatacctct gtgttcataa
     1681 ggcagggaga taaggaagac gaattaaaag caagtcttag atcgaatgag atagttgatg
     1741 tggcggaaat tgccgcttcc tttaatggag gagggcataa aagagcagca ggtttcacat
     1801 attatggaga cgaacaatca atgaagaaaa tgcttttgga aaggttggga agaactctca
     1861 atgatactta atgtctacaa gccaaaggga atatcgtcct tccaatgcgt gagtgaagta
     1921 aggagaagaa caaaggctaa aaaagctgga catgcaggca cattggaccc gaatgcggaa
     1981 ggtgttcttc cagtcatgct tgacaagtcc accagggtaa acgagttttt tttggatttt
     2041 gacaaaggct atgaatgtga aattctgttt ggcaaggaga cggatacctg cgacatatgg
     2101 ggcgaggttg ttagggaaga ggagtactca catataacag atgaagcgct attggaggtc
     2161 ataaaaagct ttcttggcca tcagaaacag attccgccgg tatactctgc aataaaagtt
     2221 gatggagttc ccatgtacaa gctggcaagg aagggtaaag cagtggaacc taaagcaagg
     2281 gatattgaga tttattcaat agacattttg gcttttgaaa gaaacattgc aagaattcat
     2341 gtgaactgtt ccaaaggtac ctacattagg tccttgtgca gggatatagg ggttgcttta
     2401 aacagcgctg catgcatgta ttctcttatt cgtacccatt acggcatgtt tgattataaa
     2461 gatgctgtca ggcttgaaaa tattaatgag aattatatgg agtatgctca tcctgtagac
     2521 ctggtgctta aacactttcc ttccgttaag ataaatgaca agcagaaagt cgattatttg
     2581 aatggcagaa gtgttttgat taatggaatt tttgaagaag agggcataag agtttatcat
     2641 aagggtgaac tattggctct agcaagaaac gagaaaacga aagatggaaa tactgtatta
     2701 aagccatgga aatattttgg tgaatagggg aagagtaatg gaagtactgt acagattatc
     2761 aaacacaaga atccctgaaa attcatacgg actgggtctt ggtaattttg acggaataca
     2821 tttgggtcat gccagaatta ttgacgtgct gtcagaagag tgtggaaaat tagggatccc
     2881 gtccgttatt tatactttca gaagacatcc cgaaaacatt cttaaaaagg acaaaaaaac
     2941 ccctatcatt ctttcaaacg aacaaaaagc atccatactg gaaaaacata atattgatat
     3001 tttatatttc gaagagttta ctgaagaatt ttccaaaata aaaccgtatg attttgtaaa
     3061 aaatattctg gttggagaat tcaatgccaa attgttggtt gtgggttacg actatacttt
     3121 tggaataaaa ggattaggaa aagcaaatga tttaattgaa ctcggaaaag agttcaattt
     3181 taaaacagtt attgtgccgc cggtggaagt tgaaggcact gcagtttcca gtacttttct
     3241 acgaaaactt atttccttgg gagacatgga gaagtataca agtatgacaa acaggcaata
     3301 ttcaattccc ggagaagtta cagtgggcag gaaggttggc tcgaaaattg gttttcctac
     3361 tgcaaatatt ctgccaagag aagggtacgc tctgccaagt gcaggtgttt atgccacaaa
     3421 aactaaactc ggtgatgtta tgtacgaaag cataacgaat atagggaaca atccaacctt
     3481 tggatgcaag cttactacaa tagagacgca tatttttgat tacgaagata aattatatgg
     3541 tcagaacatc gaagtctttt tttataaaaa actgcgggat gaaatggtgt ttaacagcgt
     3601 ggacgagctt agagctagaa ttgcaactga tatagatgat gcaaaacaat atttcaaaat
     3661 gacgagcaga aggaatggtg ttaatggaga aaatagctga gattaacggg atagacgtat
     3721 attacattcg ctcatcaaaa ttcaagacag gcagcataaa cattactttt tgcgacaatt
     3781 tgagcaagga aagggcatac aaaaacgccc ttataccatc aatcctggcg aggggctgca
     3841 aaacacaccc aacccagagg gacatctcca taaagctcat gtctctttat ggtgcgggtt
     3901 tttccaccaa tgtagagaaa aagggtgaga tacagcttat acagttcgtt gctgatttcg
     3961 tggaggaagt ttatgcaaag aactataaga atttggtgag agaactggtt gaactattgt
     4021 tcaatattat ttgcaatcct gttgtagagg gtagcggatt taaaaaagaa tattttcagc
     4081 aggagagagt gaacaataac aatattatta ggtccttgat aaacaacaaa cagagctacg
     4141 caattgtccg ctgccaggag cttatgtgca aggatgaacc ttttggcata aaggaattag
     4201 ggtctgtgga agacggagag catcttacgg aaacggaact ctacaaatat tacagcgaat
     4261 tctttttgaa gaaactgcct gtaaagatat ttttctgcgg caggaacaag cctgatgacc
     4321 taatggaagt attgaattct ttcaaggacg aatttgcagg agaaaagata aagctgaatc
     4381 ttggatacag ggaatttggc accatagaaa gaaacgatgt tgttgaaaag atggacgtga
     4441 cgcaaggcaa gctaaccatt ggattcagga ccaatgtgga tcctgacagt gacgattttt
     4501 atgctctttg tgttgccaat ggaatatttg gtgccggtgt tcactccaag ctgtttcaga
     4561 atgtaaggga gaagaacagc cttgcatatt acgccgcttc aagaattgag agatacaagg
     4621 gtctgatggt agcgtacagt ggtattgaga ttgcaaacaa ggaaaaggcg gaagagctta
     4681 tactgaagca attacaggaa gtaaaggacg gaaatgtaac tgaaaacgaa tatgagtcca
     4741 ccatcaggat gtttaaaaat gctttcaatt ctttcaagga tacccagttc gccataatgg
     4801 acttttttat agggcagaca ttcctgaata cggagatttt gcatcttgac gaatttatag
     4861 agaaggtaag caacgtgaca gtggaggatg ctgtaagatg cgcacagaaa ataaagcttg
     4921 atacggtata ttttttgaca tccaacaata tggaggttat tgaatcatga atttgagaac
     4981 aagagtcgag tttgacgaga aacttataga gtatgatcat gagtcgggtt tgcgcgtaca
     5041 ggtagtacca aagcctggat ttnnnnnnnn nnttgccata tttgctgtaa ggtttgggtc
     5101 tgttgatttg gagtttgttg ctcctatgga taatcatgtc acaaaggtgc ccgatggagt
     5161 tgcgcatttt ctggaacaca aactgtttga gcaggaagat ggaaatatgc tggataagtt
     5221 tgtatccctt ggtgcaagtc ccaatgcggc aaccagtttc aattggacct attattactt
     5281 tacctgcaca gacaggtttg aagagtgttt tggcaacctt ctttactttg tattgaatcc
     5341 atatattact gaagaaagcg tagagaagga aaagggaatt atatcccaag aaataaacat
     5401 gtatctggat gcacctgact ttatagtgtc catggagcta ttgaggctta tgtaccacaa
     5461 caaccctata aggaacgaca tagctgggtc tgttgagagt atttctaaaa ttactgcgga
     5521 tatactttta aaatgccatg aaaccttcta tcatccatca aacatggttg ttacagtagt
     5581 tggcgatgtg gatccgaagg aaattcaaga tgttgtcgac aggcatattc ccaaaactgg
     5641 gagggaaggg gatataaaga ggattgagaa ggaggagccc ctggccttga agggtactga
     5701 aagcatttta accatggatg tgtcattgcc gctattcaat attggattca aggatgatcc
     5761 tgtcaagctt tttggatctg aaagggttag aagagagatt gcaggcagca tcttaaagga
     5821 aatgatgttt ggcaaagtgt ctacttttta cgagacatta tacaacgaag gagtcataag
     5881 caatgacttc tatgcggatt atgagacggg tgcaaattac gctcttttca gccttggtgg
     5941 agaatctcaa aaaccccagc gggtaaggga atatcttgaa cagtatattg aagagattag
     6001 gaaaaacgga tttgacaaaa agtattttga aatggttaag aacgcacttt ctggccgtgt
     6061 gttgagaagg ttcaattcac cggaatccat gggtagaatg tttgcatctt catatcttat
     6121 tggtgttgat gcatttgact attttaccgc atatggtaca ataaatcttg atgagagtta
     6181 ccatatcttt gaggaagttt tcctgaaacc tatggctgtc tccattgtaa atcctaaatg
     6241 attcaggagg tttcgtgtgg ataacataat tgaatttccc aaactaggtt tagagttcca
     6301 cattaatgat gttgcatttg atgcaattgg caattttgag ataaaatggt acggaatcat
     6361 aatatgtttt ggtttcctgt taagcattat tctcgcaatg agggtttgcg agaatttcga
     6421 catctcaaaa gacgatttgc ttgattatct cttgtttgca atgcctgcag ccattgttgg
     6481 agcaagattg ttctatgtgg tttttgcttt tgatgagtac aaggacaacc taaaagaaat
     6541 attcaacata aggcagggcg gccttgccat ttacggcggc gtcatatttg cggtgataac
     6601 cgtgcttatt gtttccaagg taaagaaaaa gtcggtactg aacgtgcttg actttgccgc
     6661 accatacatc atgcttggac aggcaatagg ccgctggggc aactttacca accaggaagc
     6721 tttcggtgga gctacaaatc ttccttgggg catgacggga aatctgataa aagaaaggct
     6781 tgaatactac ggactgtcaa ggcagcagct tgtgcatccg acctttctgt atgagtcctt
     6841 gatttgcttc gttggatttg cattcatgat gatttacaga aagagatggc agaaggaaaa
     6901 gggagaaatc ctctgtctgt atatgattat atatggaatt gcaagagcga ttatcgagtc
     6961 gttgagggtt gacagcctga tgcttggcaa tgtcagagtt tcaagactta tctcaatagt
     7021 tatttttgtt cttggtatta cgcttttgat tgatattaga agaaggcaca aactgaagat
     7081 gattgagact tctgaggaag aaagttcatt aaggaaaata gtcgaggtta ttgaaacatc
     7141 cgatgaagaa gataccatgc aggaagctga agcacttgaa gctgcaagac aggctgaact
     7201 gatggcttat gaactacaag atgaatacac tgaacgggaa taaccaggtg agataattga
     7261 ggtttagcgg gattgaacgc agtcagagaa tagactactt caggaagttc gactatttgc
     7321 tgatgggtgt cgtactaata attactgcaa tagggcttgt attcctacat agtgccatgt
     7381 atgacatgta tgcagacaat ggcgcaagcg caatgacagt acagattgcc ggtgtaatac
     7441 tcggcataat cgttgcgctc tttttctgtt tttttgatta cacaaatttc agaaacgtat
     7501 gcttcccgtt ttatgttttc aatctgtttg tcatgcttct tgttttcacc cctattggta
     7561 ttgaaaacta tggcagcaga agctggatag acctttacgt taccacgtac cagccatcgg
     7621 aattaatgaa gattgcaatg attatactta ttgcaaagta cctggaaaga attaatgaaa
     7681 acggaatgac cttggacagt gcaataatca tacttgccgg attttttatt ccattgggtc
     7741 ttgtattatt gcaaaaggac ctcggcatgg cgcttgtatt cattttcatt ttccttgtaa
     7801 tgatttttgt gggtaacgta aagctttggc agattggaat aattattggt ggcggacttg
     7861 ctgcagtgcc ttttgtatgg aagttttacc ttaatggtgt aaaacgagac aggtttttat
     7921 ctttcttgaa ccctgacaag tatcaggact attcactaca attaagaagg gcgatgacag
     7981 ccataggttc aggccagctt tttggaaagg gatatatgca ggggcctttg aacagaggaa
     8041 gaaagatccc ggtaaaaatg tccgacatga tatttgcagt aatttgtgaa gagggcggat
     8101 ttatcgcagg atttgcaatt gttgtgctga tgacaattat gcttctgaga atgcttcagg
     8161 tatcacacag ggcaagggat gtttttggtt cctgtattgc agcgggcata tttgccatgt
     8221 ttgcttttaa catttttgag aacattggaa tgaatattgg cataatgccg ataaccggac
     8281 tgcctcttcc ttttgtcagc aaaggcggca gtgccatggt tacaaacttt ttttcaattg
     8341 gtttattgct aagtatatcg cttagaaata aaaaaggcat gtttatagac gactaattat
     8401 tgaataaaat atgtatcaag ggccttcctt tcgagttttt tcgattggaa ggtctttttt
     8461 ttcaaaaaaa atttaaaatt tttgaaaaaa gggcttgttt ttttatttta agtggtgtat
     8521 tatatgcatg acgggggaat ttgtggtgat tagtgtcaat cagtggcata agaatcacaa
     8581 aaaagtcccg ccaagtgcat cgaaaggagc atagtggttg tttacgggtg ctacattcaa
     8641 caccattgat gcaaaagggc gtgttgttat accacaaaaa ttccgtgaca cacttagtga
     8701 tacagggttc gttatcacta ggggtatgga tggctgtgtg tatatatacc cgaaaccgcg
     8761 ttttcaggaa cttactgaga agcttatgca gatgcagtca acaggcgtgg agacccgtaa
     8821 gctggcaagg tttctgctcg cattcgccga agaaggtgat tttgatgcac aggggagaat
     8881 ttgtgtatcc caaccacttc gtgaatacgc aggactaaaa aaagaaatac taacaactgg
     8941 aatactggat agaattgaaa tatgggacag ggagagatgg aatgcatact ctgaggaaaa
     9001 tcttgaaggt cctgatttca acgaagcagt aataggattt gatttctaac gaggtggtaa
     9061 ctatggactt tcagcatacg cccgtactgc tcgaagaaac catccgctac cttgacatca
     9121 ggcaagatgg agtatatgtg gacggtacag tgggtggtgc aggccatagt aaaaaaatac
     9181 ttgagttgct aagtgaaaaa ggattgctta tatgccttga ccaggatgaa aaagcaatcg
     9241 aggcggcggg gaagaaactt gaaggagcca aaaacgtaat actcttaaaa acgaactttg
     9301 taaatttaat atctgtttgc aattcattag gagtttccaa ggtggatggc atactgtttg
     9361 atctgggggt ttcttcacac cagtttgata ctcctgagag agggttcagt tacaggttcg
     9421 actccaaact ggatatgaga atggatgccg attctgaatt gacggcctac gatgtggtaa
     9481 acggctacga agaggcggag ctcaggagaa ttataaagga gtatggggaa gaaaggtttg
     9541 catcaaggat tacgggaaag attgtagaaa gacgaaagat aaaacctatt gaaactacct
     9601 ttgaactggt ggaggttata aaagaggcga ttcccaaaaa gcagagaatg gatggtcatc
     9661 ctgcaaaaca gaccttccaa gcaatcagga ttgaagtaaa taaggagctg gaagtgcttg
     9721 aaaaagcaat tgaagatgcc attgaaatgc tagcaccatt cggaagactt tgcataatta
     9781 ctttccattc cttggaggat agaattgtaa agacaaagtt caataatttt gaaaaccctt
     9841 gcacatgccc tccggatttt ccgatttgtg tatgtggaaa gaaaccaaag ggaaaggcgg
     9901 taacaaaaaa accaatactt ccttcccagg aggaaatgga gagaaatcca agggccaaaa
     9961 gtgccaagtt aagagtattt caatcaaact agatgaacaa tgacaaaaga aaaactttta
    10021 aaccttacga aagaaactta cagacataca aaagaaaacc aacagtttac aaaagataat
    10081 tacagtttta caaaagaaaa tttcagtttt accaaagaag aagtcttgat ttacaaaaga
    10141 aaaaaagcaa atgtacaaaa gaaaaataaa caaaaaatac aaaagatgaa ttattaaaat
    10201 tatttctacc aaagacaagc ttaaaaagcg gaggtacttg ctatgaacag tgctggagca
    10261 agggagctac ataccgaaga ttatgattac gacaatgctt atgattacgg ctacgatttc
    10321 gattacgatt atgaagagag aatagagcag tcgcctgagt ttgccgtgct cagagaactt
    10381 aagataaaga gaaacaatgc aaaggtaaag gcgaaccttg ttattaaagt ctcaataatt
    10441 attattcttg gactgataat tgcattcaga tatgcatcca taactgaact gggttacaag
    10501 gtcaatgcaa tgcaaaacca gttggatgaa atcaaggcgg aaaactcaag acttgaaacg
    10561 aatattgcaa gacagatgaa cctggctgaa attagtgaga ttgcaatcaa cgagttgggg
    10621 atgcaaaaac cccagagtta tcagatagtg agaataaatg ttgttcctac ggatcaaact
    10681 gaaattcatg agatgaaatt taccaaagat gaaaacaaca ccccctggta taaaaatgtg
    10741 ctt
//
LOCUS       DAONVY010000011        10476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_4887_length_10476_cov_4.256309, whole genome shotgun sequence.
ACCESSION   DAONVY010000011 DAONVY010000000
VERSION     DAONVY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10476
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_10476_cov_4.256309"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1340)
                     /locus_tag="PLI11_00650"
     CDS             complement(78..1340)
                     /locus_tag="PLI11_00650"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HPZ51407.1"
                     /translation="MYRNKEDVILRNYDHAKAVYKDFDVDVDKAIEKFKTIPISLHCW
                     QGDDVKGFEDLGDVESQNVVTGSYPGAARNGDELRADIEKAFSMSPAKHRVNLHSIYA
                     EPKKPTPRNELTADDFNGWIEWAKEKDYGLDFNVSFFTHPMMVDGFSLASRRKDVRDY
                     WVKAGVGGREISYEIGKRLGTPCYHNIWVPDGLKDIPANRLVYRQNLIESLDRIFEKK
                     LDRKYIRDVLEGKLFGIGIESFTVGSHEFYLAYAVKNGVGVCLDTGHYHPTETAVDKI
                     TAVAPFVDDVLLHVSRGVRWDSDHVLIQGDELDALMLEIKRGDFFDKIAIGLDFFDAS
                     INRVAAWVIGLRSAGKSILKALLEPTHLVEEAEANGDYTSRLALMEEFKNLPFNAVWD
                     YVCHTTGTYVGSTWLDEVKAYESGLNRN"
     gene            1467..2486
                     /locus_tag="PLI11_00655"
     CDS             1467..2486
                     /locus_tag="PLI11_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPZ51408.1"
                     /translation="MENIIYYNIKDEPFGIYGLYNPKEEDVFKRMPDDVAKATSKDVE
                     FHAKETAGGRIRFTTDSPYVAIKAEMPYVANFPHMPVTGTSGFDLYVHEGGKDVYKGT
                     FMPPSDMKNGYESVIHFSDRKTRNITIHFPLYNHVNKVFAGLQKGSFLGSGHRYKYEK
                     PIVYYGSSITQGGCASRPGNCYTNIISATLDCDHINLGFSGSAKAEDPIAEYIASLDF
                     AIFVYDYDHNAPTCEYLEATHERMFLRIREKQPNKPIILMSRPDFDASKESSILRRDI
                     IRKTYTNALARGDKNTYFIDGEKLFMEKYRDACTVDNCHPNDAGFVRMAEVVSSVVEM
                     LLHQF"
     gene            complement(2472..4163)
                     /locus_tag="PLI11_00660"
     CDS             complement(2472..4163)
                     /locus_tag="PLI11_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HPZ51409.1"
                     /translation="MDTKRSYSKTDIKDVKLADEYGVNPLDYVDISLINIGLSIGAIN
                     NLMKEGVHTVCDLLNLTENELYGIKNIGKRSIEIIREALEDIFKNDRKALVRRSFDII
                     VDGKKKTNKMHRREKSALEKYKDAALIAGYEISKEAYINPDKVIPIMNALSGYSDDVT
                     SMEERKKELVLNFEKIPKERHHLEIYPFIEAYSGEPEYKRVLKEIFYKKDRIIDIITK
                     ENIDDEHFYELAKFVVWLCFDISEICSSYLDDFLNDETSRKIINMRVKGKPLKTICEK
                     AEVESPRIYALEKSLLKNLRALLSRHNLVKMIVALNGGNGIVEDETLDNHFGKYKEII
                     LHYLKKINRNAISHDNSFDVYCLDAESTQLLLSIMDTFPKEIEEEELEEIIIDVAGRT
                     GVSEEMLVRMVSNRYQKTGNIYHMGKLSNLAAFSYILKKYYPEGIRINCSEELDEFYD
                     KAKELYGEDNLPKNKRVLANAVAKVGIHSYRGVYIHKDYVSYPKSVVYRIDEYIKRLD
                     RNEISAYELYIEFYEVLVKETNIKNHYMLFSILRYELENQYLFKRNFVIAKLKLM"
     gene            complement(4223..7132)
                     /locus_tag="PLI11_00665"
     CDS             complement(4223..7132)
                     /locus_tag="PLI11_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HPZ51410.1"
                     /translation="MRPNLFIDDYLDKEKDKLFLKYCRENNYQYLHQITDSDYIVFAV
                     KYKLGRQKYKEVQSRIEKIKNDAYQGKTPVNLGSDKVYDTKIMFGEKDSVAKMNLSNR
                     SYNSLLKARVRTIFDLFVKSEKEIKSIRSLGVKSFNEVMKARRMYFPEPGIVYKVVNL
                     MNERVKEEIISLLLNERPPRSNLKKEDNEIAVIEYAKESINNIGIEIARYAYKRPKET
                     LALMEALQNFSKVNLYSKDLLAKFDQIPGERRAKRLSQYTDIINDEKILLELEPLEEE
                     LEQIDKVYELKDIIPDVCADDDKRRALVVLLDYLGQSLRKTCLDDVEKTISVYTTIDI
                     EEVLVRRYNKETLQNISERYDVTREWVRQLESRAKKAIAQSLSRIGFDVFAFMVADLN
                     KDILKCEEINEYLGKDEDGEFLCNILTELVTKTTYCYDTRAGILVPKSMEKQLEKAEK
                     EVLKLEKIIPQEKIEKILTQIHNKHKIDKKILIVYFKKYYKLYGKMYCRGSLTLQTIY
                     EYILKNYYPKGIKLYEDKEIEKFKARIWELFGNIRLPKSAKAISARIVDIGVLYDRGV
                     HIHKSYVHISDELVEKIKKYIDESHKIIVSYAELFNYFKDELKEYNINNRFNLQGLLK
                     PELDKSYIMGKDYVSKEEGWTIDKEIERFVLTNGRVTKQEICDEFLGMKDIVFSTNIK
                     KNGNIIAKEDGVYIHASELDIVNEDYEKLRKLLVEHTEKIPVSARKIHVILKNNYQDF
                     LTRNDINSHSELFDVLRYMFGKEFKFSRPFIAKLGTVDACNLDVAKRILADYDVISID
                     DIVDIFNQNHIIYLSLRSILIQLNDEFLQVSKGLMGRIKEEVMTEKVKKTILNKLHSL
                     IAEKGYIAGRAVDDFKGFPDIGYPWNPYLLRSVTEKYFADKIGMVDVLTSSLTSMNTI
                     FVKKEFSDMSYSEFLEYVIKRRHEKHPFKNSKEVREWVKNERLDLKVKQTLDVLD"
     gene            complement(7357..8691)
                     /gene="mgtE"
                     /locus_tag="PLI11_00670"
     CDS             complement(7357..8691)
                     /gene="mgtE"
                     /locus_tag="PLI11_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPZ51411.1"
                     /translation="MKEHLIGLIEQNKFAQVKNTIVQMNEVDIASFLEELERPKLLMV
                     FRLLPKEIAADVFSYFSYEMQAYVVESITDKETESILDMLFLDDVVDLIEEVPSNVVK
                     KVLKNTDLERRALINQFLQYPEDSAGSIMTVEYVDLKASMTVKEALYHIKKTGVDKAT
                     INTCYVTDSRRMLEGVISIHKLILSDDDEIVGELMDRQFIYAHTLEDRESTAALFKKY
                     DLLSIPVVDNEKRLVGIVTIDDIVDVIDLETTEDMQIMAAMQPSEEAYLKTSVWNLSK
                     HRIMWLMILMFTAIVTGSIIKKYNNLLESALILSTFIPMLMDTGGNSGAQSSTLVIRG
                     LALGEIETKDVLKVVWKELRVGLLVGFALSLANFLRLILISRVGLMIAITVCISLYCT
                     VVLAKLIGGFLPILARKLKLDPAIMAGPLITTIVDMCSLTIFFNIASKLLNF"
     gene            complement(8858..10192)
                     /gene="mgtE"
                     /locus_tag="PLI11_00675"
     CDS             complement(8858..10192)
                     /gene="mgtE"
                     /locus_tag="PLI11_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPZ51412.1"
                     /translation="MKEHLIELIEQNKFAQVKNTIVQMNEVDIATFLEELERPKLLMV
                     FRLLPKEIAADVFSYFSYEMQAYVVESITDKETESILDMLFLDDVVDLIEEVPSNVVK
                     KVLKNTDLERRALINQFLQYPEDSAGSIMTVEYVDLKASMTVKEALYHIKKTGVDKAT
                     INTCYVTDSRRMLEGVISIHKLILSDDDEIVGELMDRQFIYAHTLEDRESTAALFKKY
                     DLLSIPVVDNEKRLVGIVTIDDIVDVIDLETTEDMQIMAAMQPSEEAYLKTSVWNLSK
                     HRIMWLMILMFTAIVTGSIIKKYNNLLESALILSTFIPMLMDTGGNSGAQSSTLVIRG
                     LALGEIETKDVLKVVWKELRVGLLVGFALSVVNFLRLLLISRVDLMIALAVCFSLYCT
                     VVLAKLIGGFLPILARKLKLDPAIMAGPLITTIVDVCSLTIFFNISSKLLNI"
BASE COUNT         2869 a         2372 c         1787 g         3448 t
ORIGIN      
        1 gagcgtcatt atccactacc ttgaacaaac taccgtctat atatagcttg tctatctcca
       61 gcattttgca cctcacctta gtttctattc aagccactct cgtatgcctt gacctcgtca
      121 agccacgtgc ttcccacata cgttccggtg gtgtggcata cgtaatccca aaccgcattg
      181 aaaggaaggt tcttgaattc ctccataaga gcaagtctgg atgtataatc tccgtttgct
      241 tcagcttctt caacaagatg ggtaggctca agcagcgcct tcagaattga tttgcctgct
      301 gaacgaagac caatcaccca tgcggcaacc ctgtttatac ttgcgtcaaa gaagtcaaga
      361 ccgattgcaa tcttgtcaaa gaaatctccg cgttttatct ccagcattaa agcgtcaagc
      421 tcgtcaccct ggatgagcac gtgatctgaa tcccatcgta ctccacggct tacatgaaga
      481 agaacgtcat caacaaaagg cgcaactgca gttatcttgt ccacagccgt ctcggttggg
      541 tgatagtggc ccgtgtcaag acatacacca acaccgttct ttactgcgta tgcaagataa
      601 aactcatggg agccgactgt aaagctttca atgcctatac caaacaattt tccttcaaga
      661 acatctctta tatattttct atctagtttc ttttcaaaga ttctgtccag tgactcaatc
      721 aggttctgac ggtataccag acggtttgca ggaatatcct taagaccatc cggaacccag
      781 atgttgtgat agcagggcgt tcccaatctc ttacctattt catacgaaat ctctctacct
      841 ccaacacctg ccttgaccca gtaatccctt acatccttcc ttcttgaagc aagggagaaa
      901 ccgtccacca tcatcgggtg agtaaagaag gacacattga aatcaagacc gtagtctttt
      961 tcctttgccc attcaatcca tccgttaaag tcgtcagctg taagttcgtt tctgggagta
     1021 ggtttcttgg gttctgcata tatactgtgc aggttaaccc tgtgcttggc aggagacatg
     1081 gaaaaggcct tctctatatc cgctcttagc tcgtctccgt ttctagcagc acctggataa
     1141 ctgcctgtaa ctacgttctg actttccaca tcgcccaaat cttcaaatcc ctttacatca
     1201 tcgccctgcc agcagtgcag tgaaatgggg attgttttaa acttttcaat agccttgtcc
     1261 acatcaacat caaaatcttt gtagactgct tttgcatgat cgtaatttct caagattacg
     1321 tcttccttgt ttctgtacat aaagaaccct cctgaattat caatgttgtg attattatat
     1381 cacataatgg cggttaaaca cacatgcttg caactaatct ttccaaagtg ctatactttt
     1441 ggtatcaaaa tgaaggagca gaatacatgg agaatatcat atattacaac attaaagacg
     1501 agccctttgg tatatatggt ttgtacaacc caaaagaaga agatgttttc aagagaatgc
     1561 ctgatgatgt ggcaaaagcc acaagcaagg atgtggaatt ccacgccaag gaaacagccg
     1621 gtggaagaat cagatttaca accgactcgc catacgtcgc aataaaggca gagatgccgt
     1681 atgtggccaa ttttccgcac atgcctgtaa ccggtacatc gggttttgac ctttatgttc
     1741 atgaaggtgg aaaagacgta tacaaaggaa cattcatgcc gccttccgac atgaaaaacg
     1801 gatatgaatc ggtcatacat ttcagtgaca ggaaaacacg gaacataacc atacactttc
     1861 ctttatacaa tcatgtgaac aaggtttttg caggacttca aaagggctct tttcttggca
     1921 gcggccacag gtacaaatac gaaaaaccta ttgtgtatta cggttcctcc atcacacaag
     1981 gaggctgtgc ttcccgtcca ggcaattgtt atactaatat tatttcagca actcttgact
     2041 gcgatcatat aaatttaggg ttctcaggct ctgcaaaagc agaagaccca atagctgagt
     2101 atattgcatc tcttgatttt gccatctttg tatatgatta tgaccacaac gctcctacct
     2161 gcgagtacct tgaggcaacc catgaaagga tgtttctgag aatacgagaa aaacagccaa
     2221 acaaaccgat tatactgatg tcaagaccgg attttgacgc ctctaaagag tcaagcattt
     2281 tacgcaggga tataatacga aagacataca caaatgcatt ggcaagagga gataaaaaca
     2341 catactttat tgacggagaa aaactcttca tggaaaaata tcgtgatgca tgcacagtgg
     2401 acaactgcca tcctaacgat gcaggatttg tgcgcatggc tgaagttgtt tcttctgttg
     2461 ttgaaatgct tttacatcag ttttagcttt gcaattacaa agtttctctt gaacaggtat
     2521 tggttttcaa gttcgtacct tagtatgctg aataacatgt agtggttctt tatattagtc
     2581 tcctttacaa ggacttcgta aaactcaata tacaactcat atgcacttat ttcattccga
     2641 tccagtcttt ttatatactc atctattcta taaaccacgg atttgggata ggagacataa
     2701 tctttatgta tgtacacacc tctgtagctg tgaataccca ccttcgccac cgcatttgca
     2761 agaacacgct tgttctttgg caagttgtcc tcgccataaa gctcctttgc tttgtcatag
     2821 aattcatcaa gctcttcact gcagtttatt cttatgcctt caggataata ttttttaaga
     2881 atatagctaa aggcggccaa attggaaagc ttccccatat gatagatatt gcccgtcttt
     2941 tggtacctgt tggataccat tcttacaagc atttcttccg aaacacccgt tctgcctgcc
     3001 acatcaataa ttatttcttc cagctcctct tcctctattt ccttcggaaa cgtatccatt
     3061 atactcagta atagctgcgt gctttccgca tcaaggcaat atacatcaaa ggagttgtcg
     3121 tggctaatgg cattcctgtt aatcttctta aggtaatgca gtataatctc cttgtatttt
     3181 ccaaaatggt tatcaagggt ttcatcctcc acaatgccgt ttcctccatt taaagcaaca
     3241 atcattttaa cgagattgtg ccttgaaaga agagctctca agttctttaa cagagacttc
     3301 tccaatgcat atattctagg ggattccacc tccgccttct cacaaatggt tttcaaaggc
     3361 tttcctttaa ctctcatatt tattatcttc cttgatgttt catcgtttaa gaaatcatca
     3421 aggtaggacg aacatatttc acttatatca aaacagagcc agacaacgaa tttggcaagt
     3481 tcataaaagt gttcgtcatc tatattttcc ttagttatga tgtcaattat ccggtccttt
     3541 ttgtagaata tttccttaag aactcgtttg tactcgggtt cgcctgaata ggcctctata
     3601 aaggggtata tttccagatg gtgcctttct ttcggtattt tttcaaaatt caacacaagc
     3661 tcctttttac gttcttccat gcttgtaaca tcatccgaat agccgcttaa agcattcata
     3721 atcggaataa ccttgtccgg atttatatac gcctctttgc tgatttcgta ccctgctatt
     3781 aatgcagcat ccttgtattt ttcaagagca ctcttttctc tcctgtgcat cttgtttgtt
     3841 ttcttctttc catccacaat aatgtcaaaa cttcttctaa ccagcgcttt cctatcattc
     3901 ttaaaaatat cttccaaagc ttccctgatt atctcaatgc tcctcttccc tatattcttg
     3961 atgccgtaca actcgttttc agtgaggtta agcaaatcgc agacggtatg caccccttcc
     4021 ttcataagat tgttgattgc tccaatagac aacccaatgt taatcaagct gatatcaaca
     4081 taatccagag gatttacacc atactcatcc gccagcttaa catctttaat atctgttttt
     4141 gaatacgatc ttttcgtatc catatgccac ctgcttcccg gatacttaca ttcttgcata
     4201 ttttgttttt aagaactgga tgttaatcca gcacatccag tgtttgtttc accttcaaat
     4261 caagacgctc attcttcacc cattccctta cttccttact gttcttgaat ggatgcttct
     4321 catgacgtct tttaataacg tactcaagga attcggaata ggacatatca ctgaattcct
     4381 tttttacaaa tatggtattc atgctggtaa ggcttgaggt aaggacatcc accattccaa
     4441 tcttgtcggc aaaatatttt tccgttacgc ttcttaacag gtacggattc cacggataac
     4501 caatgtcagg aaatcccttg aagtcgtcca ctgcccttcc tgcaatgtat cctttttcag
     4561 caatgaggga atgtagcttg ttaagtattg ttttcttaac cttctccgtc attacctctt
     4621 ccttgattct gcccattaag ccttttgata cctgcaaaaa ctcatcatta agctgaatca
     4681 gtatgcttct taatgaaagg taaattatgt ggttctgatt aaagatatcc acaatgtcat
     4741 ctatgcttat gacatcgtaa tctgcaagaa ttctctttgc aacatccagg ttgcatgcgt
     4801 ccactgttcc cagtttggct atgaatggtc ttgagaactt aaactccttt ccaaacatgt
     4861 atctcaggac atcaaacagc tcactgtggg agtttatatc gttccttgtt aagaagtctt
     4921 gataattgtt tttcagaatt acatgaatct ttcttgcgga aacgggtatc ttttccgtat
     4981 gctccacaag aagttttctt aacttttcat aatcttcatt aacaatgtcc agttctgatg
     5041 cgtgtatgta tacaccgtct tccttggcta ttatgtttcc attcttttta atatttgtag
     5101 aaaatacaat gtccttcatt ccaaggaact catcacaaat ctcctgcttt gtgacccttc
     5161 cgtttgtcag gacgaatctt tctatctcct tgtcaatggt ccagccttcc tctttggata
     5221 cgtagtcttt tcccattatg taactcttgt caagctcagg ctttaaaagt ccctgcaggt
     5281 tgaacctgtt gttaatgtta tactccttca attcatcctt gaagtagttg aagagttccg
     5341 cataggatac tattatcttg tggctctcgt ctatgtattt ctttatcttc tctacaagtt
     5401 cgtcacttat atgaacatag gatttgtgta tatgaactcc cctgtcataa agcaccccaa
     5461 tatccacaat gcgggcagaa atcgccttcg cacttttagg tagtcttatg tttccaaaaa
     5521 gttcccatat tcttgccttg aacttttcaa tttccttgtc ttcatagagt ttaatgccct
     5581 ttgggtagta gtttttcaga atatattcat aaattgtctg cagtgtaaga ctgcccctac
     5641 agtacatctt gccataaagc ttgtaatatt ttttgaaata gactataagt attttcttgt
     5701 caattttatg tttgttatgt atttgggtca gaattttttc tatcttttcc tgtggtatga
     5761 ttttctcaag ttttaatact tccttctcag ccttctcaag ctgtttctcc attgattttg
     5821 gcacaagaat tcctgctctt gtgtcatagc aatatgtggt ttttgtaaca agttcggtta
     5881 gtatgttgca caagaattca ccgtcctcgt ccttgcccag gtactcatta atttcctcac
     5941 atttcaatat atccttattc aaatcagcaa ccatgaatgc aaacacgtca aagccaatcc
     6001 tgctcaaact ttgtgcgatt gccttctttg cacgggactc aagctgcctt acccactccc
     6061 ttgtcacatc gtacctttcc gatatgttct gaagcgtttc cttattgtac ctccttacaa
     6121 gaacctcctc gatatctata gtcgtataca cactgatggt cttctctaca tcgtccaggc
     6181 atgttttcct gagactctga ccaagatagt caagcaacac cacaagcgca cgccttttat
     6241 catcatcagc gcacacatca ggtatgatat ccttcaattc ataaactttg tcaatctgct
     6301 caagttcttc ttcaagaggt tccagttcca gcaaaatctt ctcatcattg attatgtccg
     6361 tatattgaga aagccttttt gcccgtcttt cgcctgggat ctggtcaaac tttgcgagca
     6421 ggtcttttga gtacaagttg acctttgaga agttttgaag ggcttccatt aatgctagcg
     6481 tttcctttgg acgtttgtat gcataacgcg caatttcgat tccaatattg tttattgatt
     6541 ccttggcata ttcaattacg gcaatttcat tgtcttcctt tttgagattg cttcttggtg
     6601 gcctttcatt caacaaaaga cttattatct cttccttgac cctttcattc ataaggttta
     6661 ccaccttgta tacaatcccg ggttcaggaa agtacattct tctcgccttc ataacttcgt
     6721 taaaagattt tacccctaga gaccttatcg attttatttc tttctcgctt tttacgaata
     6781 gatcgaagat ggttcgcact ctcgccttta gaagggagtt gtacgaccgg ttggataggt
     6841 tcatttttgc aacgctgtct ttttcaccga acattatctt tgtatcatac actttgtcag
     6901 aacccaggtt tacaggagtc ttgccttggt atgcatcgtt cttgattttc tcaatgcggc
     6961 tttgcacctc tttatacttc tgtcttccca gcttgtattt gactgcaaac actatgtagt
     7021 ccgagtctgt gatttgatga agatactggt agttgttctc cctgcagtat ttcaaaaaca
     7081 gcttgtcttt ttctttgtcc aaatagtcat ctataaacaa attgggccgc aaaaattatc
     7141 acccgcctgc ttttgttgtt cctttattaa aattttgcat taactagtgc tttacatacc
     7201 tattgtataa caagttgtac tcaattgcaa cacttatttt acaattatac agacatttaa
     7261 gaaaaaatca aatgtgtttc gcaattataa ggcgattttc agcaaatatt ttacaaattg
     7321 acataaaaat aaccggttta atcaaaccgg tcatttttaa aaattcaaaa gtttagatgc
     7381 aatattaaag aatatggtaa gcgaacacat gtcaacaatt gttgtaatga gcggacctgc
     7441 catgattgca ggatcaagtt ttaactttct tgcaagaatc ggcagaaaac ctccaataag
     7501 ctttgccagc accaccgtac aatacaaact tatgcacacg gtgattgcaa tcatcaagcc
     7561 aaccctgcta attaatatta gtcttaggaa atttgcaaga ctcaatgcaa agcccaccaa
     7621 aaggccaact cttaattcct tccaaacaac tttaagtaca tcttttgtct caatctcgcc
     7681 caatgcaaga cctcgaatta ccaacgtaga tgactgcgca cctgaatttc ctcctgtgtc
     7741 catgagcata ggaatgaatg tggacagaat aagcgcagac tcaagtagat tgttgtactt
     7801 tttgataatg ctgccagtta cgattgcggt aaacatgagt atcatgagcc acatgattct
     7861 atgctttgat aaattccaca cactggtttt cagatatgct tcttccgaag gctgcatggc
     7921 cgccattatc tgcatgtcct ccgttgtctc taaatcaata acgtctacaa tgtcgtcaat
     7981 cgttacaatt cctactaacc ttttttcgtt atcaacaaca ggtatggaca aaaggtcgta
     8041 ctttttaaac agtgcagctg tagactccct gtcttcaaga gtgtgtgcgt aaatgaactg
     8101 tctgtccatt agttccccca ctatctcatc atcatcgctg agaattagtt tgtgtatgct
     8161 gataacgcct tcaagcattc tcctggaatc agtgacataa caggtattga ttgtggcttt
     8221 gtcaacgccg gttttcttga tatggtaaag ggcttccttt accgtcatgc tggctttcaa
     8281 atcaacatac tccacggtca tgatgctgcc tgccgagtcc tcgggatact gcagaaactg
     8341 attgataagc gctcttcttt caaggtcagt atttttcaaa acctttttaa cgacattgga
     8401 aggcacttcc tcaatcaaat ccacaacatc gtcaagaaac agcatgtcaa ggatgctttc
     8461 cgtttctttg tctgttatgg attcaactac atatgcttgc atttcatagg agaaatagga
     8521 gaacacatcc gctgcaattt ctttaggcag aagtctgaaa accatcagaa gtttgggtct
     8581 ttcaagttct tccaaaaagg atgcgatatc tacctcattc atttgcacaa tcgtgttttt
     8641 cacctgtgca aacttgtttt gttcaataag tccgatcaga tgttctttca tttttagcgc
     8701 tcctttcaag agagctaaaa acttaaagtg gtagtttcta gccctccaat attattttgt
     8761 tttttaagtt gattgaaact acgttcagga ttaggactgg aatccaaaac taccaccacc
     8821 ttcaactata ttatttttgc caagatcaca aagtgtatta aatattcagt agtttcgatg
     8881 aaatattgaa aaatatggtg agcgagcaaa cgtccacaat cgttgtaatg aggggacctg
     8941 ccatgattgc aggatcaagt tttaactttc ttgcaagaat tggcagaaaa cctccaataa
     9001 gctttgccag caccaccgta cagtacaaac taaaacatac ggccaaagca atcatcaagt
     9061 caaccctgct gattaacaac aatcttaaga aattcacaac acttaacgcg aagcccacca
     9121 aaaggccaac tcttaattcc ttccaaacaa ctttaagtac atcttttgtc tcaatctcgc
     9181 ccaatgcaag acctcgaatt accaacgtag atgactgcgc acctgaattt cctcctgtgt
     9241 ccatgagcat aggaatgaat gtggacagaa taagcgcaga ctcaagtaga ttgttgtact
     9301 ttttgataat gctgccagtt acgattgcgg taaacatgag tatcatgagc cacatgattc
     9361 tatgctttga taaattccac acactggttt tcagatatgc ttcttccgaa ggctgcatgg
     9421 ccgccattat ctgcatgtcc tccgttgtct ctaaatcaat aacgtctaca atgtcgtcaa
     9481 tcgttacaat tcctactaac cttttttcgt tatcaacaac aggtatggac aaaaggtcgt
     9541 actttttaaa cagtgcagct gtagactccc tgtcttcaag agtgtgtgcg taaatgaact
     9601 gtctgtccat tagttccccc actatctcat catcatcgct gagaattagt ttgtgtatgc
     9661 tgataacgcc ttcaagcatt ctcctggaat cagtgacata acaggtattg attgtggctt
     9721 tgtcaacgcc ggttttcttg atatggtaaa gggcttcctt taccgtcatg ctggctttca
     9781 aatcaacata ctccacggtc atgatgctgc ctgccgagtc ctcgggatac tgcagaaact
     9841 gattgataag cgctcttctt tcaaggtcag tatttttcaa aaccttttta acgacattgg
     9901 aaggcacttc ctcaatcaaa tccacaacat cgtcaagaaa cagcatgtca aggatgcttt
     9961 ccgtttcttt gtctgttatg gattcaacta catatgcttg catttcatag gagaaatagg
    10021 agaacacatc cgctgcaatt tctttaggca gaagtctgaa gaccatcagg agtttgggtc
    10081 tttcaagttc ttccaaaaag gttgcgatgt ctacctcatt catttgcaca attgtgtttt
    10141 tcacctgtgc aaacttgttt tgttcaataa gttcaatcag atgttctttc atttttagca
    10201 ctcctttaag agagctaaaa acttatagtg gtagtttcta gccctctaat attattttgt
    10261 ttttcgattt gtttgaaaca cggttcagga taaggactgg aatccaaaac taccaccacc
    10321 ttcatacctc atctttatta ttttattcat tgcattatat tattctacta catttatcga
    10381 taaagtcaat gttatgaagt tcctttttat gtaaacggtc attttaaatg caaaggcttc
    10441 tttgcattta cttcttccac atgttatggt ttagta
//
LOCUS       DAONVY010000012        10343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_4959_length_10343_cov_3.768177, whole genome shotgun sequence.
ACCESSION   DAONVY010000012 DAONVY010000000
VERSION     DAONVY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_10343_cov_3.768177"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLI11_00680"
     CDS             <1..217
                     /locus_tag="PLI11_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ51413.1"
                     /translation="LSTETPELSAAGVAFYEGISGNPQEMEALTFYNSITKGTPLVVE
                     PEQACVVSQILEAIYISAKTGKPYYFS"
     gene            232..606
                     /locus_tag="PLI11_00685"
     CDS             232..606
                     /locus_tag="PLI11_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51414.1"
                     /translation="MDRINLQLYSVREDAGKDFRGTLEKVARTGFTGIEFAGFYGVDA
                     KIMKKWLEELGLEAVSSHSNVFDNLDEEIEYLCTVGAKHIVLPSANFDNRDVVLKLAE
                     DLNRIGEVQATWLESWLSQPQF"
     gene            554..952
                     /locus_tag="PLI11_00690"
     CDS             554..952
                     /locus_tag="PLI11_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51415.1"
                     /translation="MKCKRHGLNLGYHNHSFEFKKDEDGKWLLDVLFENTDPNLVSVQ
                     LDVCWAHVGGVDPVKYLKKYKDRALTVHMKEVKTVSPYEGTAIGLGIVDFAGIYNLLG
                     DECDYIIEQEGIAMDTWEGLSMSVDYLRKI"
     gene            952..1260
                     /locus_tag="PLI11_00695"
     CDS             952..1260
                     /locus_tag="PLI11_00695"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPZ51416.1"
                     /translation="MSFFKSVYEAVKLIPKGRVATYGDIARYVGSPRAARVVGYALHV
                     NPEPNAIPCHRVVNREGRLAPSFAFGGMEVQKALLEAENVEVSDDGYVDLAKYGFKFA
                     "
     gene            1333..3006
                     /locus_tag="PLI11_00700"
     CDS             1333..3006
                     /locus_tag="PLI11_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPZ51417.1"
                     /translation="MGENENKEMENRQGFTNSYKNLIISLVLIIIVFTLGIVAIDLFT
                     ETPHKGDSIINTPNIDATPKPIPDVNDIRQLSNDLLNLEANEKEWAKKDEDPEDEGLY
                     LISENNDKFLVYHIDSENIADGGYTLYDFTYMWINSEDDDFYAVINISGKRVDLSNYY
                     ILVRDETRNYASRAIINCYEATEVILNNTILTGTIIAPNAHVYYEDTYVYGQVHAKSY
                     EGTMKFYREIVFTGYANLMYDLVYADIKVDAVRIAAIEFLEENDTLGTYSHYTSESKL
                     RLADTQVITSLVLDDKDVEEIGSDLAFFENLTHLSINNTNLSRIDLTYAPGLKYLSIN
                     DTSVAELDISHTPYLEYLSAFNTEISSLDLSNTPNLYYMNLDDTNIEELDTRNLQMLL
                     NLSIRNTPIKDIDLSSNTKLINYACAGTILPVPDLGSLKNLEYLDLSNTNLESIEIGS
                     DSLKVLDISRNEKLSSFDFSLFPNLEKLDISSCNISEFNFEKAGKLKVLNASFNKFST
                     IDLSELPTLTNAELYGDTVKTIIAKSYVKIYCDSKVEVRWSDYNGGEEA"
     gene            2987..3439
                     /locus_tag="PLI11_00705"
     CDS             2987..3439
                     /locus_tag="PLI11_00705"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPZ51418.1"
                     /translation="MEAKKPRDSITKMEQVVLASHANGQGRLFGGQLMSWLDVTGAIC
                     ARRHSGCEVVTAKADNLEFHRPAMQNDIVTITAKIIYAGTTSMEVRVCAYVEPKGGKE
                     PQLICSSYFVYVAMDENGNKCRVPKLIPESKEEEEDLRRCEEKRNKLR"
     gene            complement(3431..5221)
                     /locus_tag="PLI11_00710"
     CDS             complement(3431..5221)
                     /locus_tag="PLI11_00710"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPZ51419.1"
                     /translation="MRAYKIALKSIFALIVLLLTVIFLISITGSNGEEVKPEHTYRIV
                     PSYNPGFYPHDLSLSLYILNEDDEVVDDKDLEILYTMTNHNARTPQKGELITKTGEVY
                     MFSGGKEPDYEYMDFAGIRKTYKYTKSIDVINTSNNFPRVVVVRAAIFADGKKHGRTQ
                     TYTYIIGTGIEDFAEHFGAMVVCITTDEKNLYGYEEGIMIKGATYDFTKINNPQRDED
                     WWYPKNYNQRGRQWERKAHVDFFESDGTLVLSQDCGIRTAGGTSRNAFIKSLKLIARR
                     EYTEHTGTFDYEFFDDLRDHYGNRVKRFEKLVLRNSVNDDGGSMIRDQLINDLGAYAG
                     VDHQAGRPCVVYMNGKYYSMMTLKQSLDNDNIETRYHINKDMLAIITIQSDFFQFRYR
                     LESGTEEDFNSYIKDMRKLINTNYANKDISEMEEIIDVDNFIKYMAFQIYIVNPDWPH
                     NNVMAWKYTGPVNESVYGMDGKWRFVLRDLDFGFRDPSRNMFETVMSGGLYNNEPHLG
                     QVLKNLLRNKEFNRRFKEYMTKLCTEILTEEVLTEYILNAKEERVQDMKMYRFYFGGS
                     DLQWNNLINEFLDFAKQRNRHVLNQLKNLP"
     gene            complement(5225..5878)
                     /locus_tag="PLI11_00715"
     CDS             complement(5225..5878)
                     /locus_tag="PLI11_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPZ51420.1"
                     /translation="MRIVFLGDSVTEGCFELYDVEGGFDTVRDYGNVYHNKLKKLWTQ
                     KYGVEPLEVINSGISGDSVDGAVARLSKDVLEHNPDTVVVCFGLNDVCRGRDNLDNYI
                     SKLEHIFTQIQDKGIRVIFMTPNMMNTYVHDETLNSAKEAAKTTAAMQNGGTFDLYIE
                     SAKALCKRMNVTVVDAYSKWKQMHEEGIDTTVLLSNLINHPTREMHNLFANMLIEVL"
     gene            6030..6105
                     /locus_tag="PLI11_00720"
     tRNA            6030..6105
                     /locus_tag="PLI11_00720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6063..6065,aa:Arg,seq:cct)
     gene            6333..6956
                     /locus_tag="PLI11_00725"
     CDS             6333..6956
                     /locus_tag="PLI11_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51421.1"
                     /translation="MVIAYVLHLMDYIPGLKTVLDRGLFDEKYMLWIGNTEIIMMALA
                     GMFGLLCLKVNSLRHVRNVFLILLVLFMLASSSVLFFFTEDKVPHGGNALLSENMHYL
                     MIRYAAIGGVLAIILYVIAVFAKDRIFSIIATIVGVAGLLFSLMAFTLTRISIFENLV
                     EQSKSYRFDSVQYYPMLLFHLGSILVIVSLLHLSRVAIKKGDETEIP"
     gene            complement(6958..7326)
                     /locus_tag="PLI11_00730"
     CDS             complement(6958..7326)
                     /locus_tag="PLI11_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51422.1"
                     /translation="MDIKVYRSDNTSTKENDINIVNILGYIKLNPKNASLKSISKLFN
                     YSPSYMSRKIKETTGLTLTEIISKYRLNNACLLLRNTMLPIDDIVNLTGYPTRELFYR
                     HFKKEMKCTPAQYRKKSLST"
     gene            complement(7341..8009)
                     /locus_tag="PLI11_00735"
     CDS             complement(7341..8009)
                     /locus_tag="PLI11_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HPZ51423.1"
                     /translation="MDIRAAIDASIVFTEDELYNLKYMELKKLVKSSEDIPDVCARFS
                     YPLERFMYYENMQHYTPYYTEEEAFEKTSDNIVVIRHARYMPSCMHSHSFVEIPYVYR
                     GTCINILGGNRIQMYEGDFCIMGPDVKHSIEIDSDSVLMNIIVRKSNFKKLFYSILCS
                     DNIISNYFNDTVEHNNPGIIFCPAGDNIQIRSFLDTLIYQQYTPMNCHHPLRTVFSIS
                     FWRT"
     gene            8201..8860
                     /locus_tag="PLI11_00740"
     CDS             8201..8860
                     /locus_tag="PLI11_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ51424.1"
                     /translation="MCKSRVLILVVFIAAVAVFLGACDSESNSDGNVKPTVNQTPKIT
                     AAPTVTNTPSPTPNIGNNVALYKKTQVSSTVDESYLQWGWGQEFINDGTLELSFTSAV
                     KRNMDSPDVEEWIIIDLEKEYSIHLVILYPRQDNGRGFPEDYQIDVSSDNETWETVYT
                     KTEDKGSEDEDVDPREIELEAVSARYIRLLATKLTDVPDTSDGYLLQMAEMEVYSFDE
                     E"
     gene            8847..10190
                     /locus_tag="PLI11_00745"
     CDS             8847..10190
                     /locus_tag="PLI11_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51425.1"
                     /translation="MMKSKAYYFTKKGLVLLIMLAVICSAGCSSSSRGDLKKIYDEYE
                     GNTLLIGGWVTPRGEFVKTAEEAEARYKELADAGINMMYTFDDTDDPDRLKRVMDAAE
                     KSGIKLVVHLDLNNFNHSLDIVNMTKDHPAVIGYILKDEPPASLFPGLLALRKKVYDI
                     VGDDKIIMINLLPNYASDAQLGVVPKDGKTSYQIYVEQSMKIVNPTLLCTDYYMFRAG
                     KDGDPGVLAGYYRNMFDLRDAANEYEVDLWGFLQNSGWAGMREPNKAELRFSAHVNLA
                     FGVKCLAYFLCWNPHADSIKGDGRYDPTSMISYDGKITDNYYKVQAINKELHAMKGVY
                     LDYKHKGIIMYEKTPFVKSTIPEENLLESFNELVSLDAARNTLVGCFEKEGKTGLYVV
                     NCDYSDNGVNTVKLKLNDKHTYRIWSKDGLIAQKKSDTVSLKLIPGEGVFVQIID"
BASE COUNT         3053 a         1842 c         2200 g         3248 t
ORIGIN      
        1 acttagcacc gagacgccag aattaagcgc tgcaggtgtt gcgttctatg aaggtatttc
       61 cggaaatcca caggagatgg aagcccttac attttacaac agcattacaa aaggcactcc
      121 attggtagta gagcctgaac aagcatgtgt tgtttctcag attcttgagg cgatttacat
      181 ttcagcgaaa acaggcaaac catactactt ttcataagga gataatatac aatggataga
      241 ataaatctgc agctgtactc ggtcagagag gatgcgggaa aggactttag aggcactctg
      301 gagaaagtgg caaggacagg ctttacaggc attgaatttg ctggctttta tggtgtggat
      361 gccaaaatca tgaaaaaatg gctggaagaa ctcggtcttg aggctgtaag cagccactcc
      421 aacgtgtttg acaatcttga tgaggagatc gagtaccttt gtactgtagg tgcaaaacat
      481 atagtattgc catctgccaa ttttgacaac agggatgttg ttctgaagct ggctgaggat
      541 ctcaacagaa taggtgaagt gcaagcgaca tggcttgaat cttggttatc acaaccacag
      601 ttttgagttt aaaaaagatg aggacggcaa atggctttta gatgttttgt tcgagaatac
      661 agaccctaat ttggtgtctg tgcagctgga tgtttgctgg gcgcatgtag gcggtgtaga
      721 cccggtgaag tatcttaaaa aatacaagga cagagcactt actgttcaca tgaaggaagt
      781 aaagacagtg tcaccctacg agggtactgc aataggtctt ggcatagttg actttgcggg
      841 catatacaat ttactgggtg atgaatgtga ctacattatt gaacaggaag gcattgccat
      901 ggatacctgg gaaggtctgt ccatgagcgt tgattattta aggaagattt aatgagtttt
      961 tttaagagtg tttatgaagc tgtcaaacta ataccaaaag gtagggttgc cacttatggg
     1021 gatattgccc gatatgttgg cagccctcgt gctgcaagag ttgtaggcta tgcccttcat
     1081 gtaaatcctg agcccaatgc tattccatgt catagggttg tgaacaggga agggcggctt
     1141 gctccctctt ttgcttttgg cggcatggaa gtgcaaaaag ctttattgga agctgagaat
     1201 gtggaagtaa gtgatgatgg atacgtggac cttgcaaagt atggcttcaa gtttgcctga
     1261 tttgccaaag tcggtttttt tatgctacaa tagcttctac atttattgtt atcagttcgg
     1321 gggtattgcc tgatgggcga aaatgaaaat aaagaaatgg aaaataggca gggatttact
     1381 aatagttata aaaacttgat aatatcgctt gttctgatta ttattgtctt tactttagga
     1441 attgttgcaa ttgacctgtt tacggaaaca ccccataaag gtgatagtat tatcaatacg
     1501 ccgaatattg acgcaacccc aaaacctatt ccagacgtaa atgatatacg ccagttgtct
     1561 aatgatcttc taaatctgga agcaaatgaa aaagagtggg ctaaaaagga tgaagaccct
     1621 gaagatgaag ggttgtacct gatatctgaa aacaatgaca aatttctagt ttatcatatt
     1681 gacagtgaga atatagcgga cggaggatac actctgtacg atttcacata tatgtggata
     1741 aattccgagg atgatgattt ttatgccgta atcaatattt ccggcaaaag agttgacttg
     1801 tccaattatt atattcttgt aagggatgaa acaaggaact atgcttcccg tgcaataatc
     1861 aattgctatg aggccactga agtaattttg aacaacacaa tcctgacagg cacaataatt
     1921 gcaccaaatg ctcatgtata ctatgaagat acttatgtat acggacaggt gcatgccaaa
     1981 agctacgaag gcacaatgaa gttttacagg gaaattgtct ttactggata tgcaaatttg
     2041 atgtatgacc ttgtttatgc agacatcaag gttgacgcag taaggattgc tgcaattgag
     2101 tttttggagg aaaacgatac tttgggtaca tactcccact atacatccga aagcaagttg
     2161 agacttgcgg acacacaggt cattacttct ctggttctgg acgacaagga tgtggaggag
     2221 attggctctg accttgcctt ttttgagaat ctgacccact tgtccataaa caatacaaac
     2281 ctatccagaa tagatctcac atacgcaccg ggactaaagt atttaagcat aaacgataca
     2341 agtgttgccg agcttgatat ttcgcacaca ccataccttg aatacttgag tgcgttcaac
     2401 actgaaatat catcccttga cctgtccaat actccaaatt tgtactacat gaatctggac
     2461 gacaccaaca ttgaagaact tgatacaaga aacttgcaaa tgcttctcaa tttaagcatt
     2521 aggaacactc cgataaagga tattgacctt tcttccaata ctaaactaat aaattacgct
     2581 tgtgcgggaa caatattacc ggttcctgat ttgggttctt taaagaatct tgaatacctg
     2641 gatctaagca ataccaatct tgaaagcata gagattggct cggattcgtt gaaggttttg
     2701 gatattagca ggaatgaaaa gctttcaagc tttgactttt cactgtttcc aaatctggag
     2761 aaactagata tatccagctg caatatttct gagtttaact ttgagaaggc tggcaaacta
     2821 aaagtgttga atgcaagttt caataaattc agtacaatag acttaagtga actgccaaca
     2881 ctaacaaacg ctgagttata tggagatacg gtcaaaacaa taatagcaaa aagttatgta
     2941 aaaatatatt gtgacagcaa agtagaagta agatggagtg attacaatgg aggcgaagaa
     3001 gcctagggat tcaataacca agatggaaca agtggtactg gcttcccatg caaatggtca
     3061 gggaagactt tttggaggac agctcatgtc ctggcttgat gttaccggtg ccatatgtgc
     3121 aagacgacac agcggttgcg aggttgttac tgcaaaagct gataatctgg aatttcatag
     3181 acctgccatg cagaatgaca tagttacaat taccgcaaag ataatttatg ctggcaccac
     3241 atccatggaa gttagagttt gtgcatatgt tgagccaaaa ggaggaaaag agcctcagct
     3301 catatgctca agttattttg tttacgttgc catggatgaa aacggcaaca aatgccgggt
     3361 accaaagctg atacccgaat caaaagagga agaggaagat ttgagaagat gtgaggaaaa
     3421 gagaaacaag ctaaggtagg ttttttaatt ggtttaaaac atgcctgttt ctttgcttgg
     3481 caaaatccaa aaattcattt ataaggttgt tccactgcaa atcgcttccg ccaaagtaga
     3541 acctgtacat tttcatgtcc tgaacccgtt cttctttggc gtttaatata tattcagtca
     3601 agacttcttc agtcagaatc tcagtacaaa gctttgtcat atattcctta aaccttctgt
     3661 tgaactcctt gtttctaaga aggtttttca atacctgtcc caaatggggt tcgttgttgt
     3721 ataatccgcc tgacatgacc gtttcgaaca tgtttcttga tggatcccta aaaccaaagt
     3781 ccaggtctct aagtacaaac ctccattttc catccatgcc atagacactt tcgttgacag
     3841 gacctgtgta tttccatgcc ataacattat tgtgaggcca atcagggttg acgatatata
     3901 tttgaaaagc catgtatttg ataaagttgt ctacatcgat aatctcttcc atctcggata
     3961 tgtccttgtt tgcgtaattg gtattaataa gtttgcgcat gtctttaatg tatgagttaa
     4021 agtcctcttc ggtgcctgat tccagcctgt atctgaactg gaagaaatcc gattgtatgg
     4081 ttattatagc cagcatatcc ttgtttatat gataccttgt ttcaatgttg tcgttgtcaa
     4141 gggattgctt aagtgtcatc atggaataat atttgccatt catgtaaaca acgcagggtc
     4201 ttcctgcctg atgatctact ccagcgtaag cgccaagatc gtttatgagc tggtctctta
     4261 tcatggagcc accatcgtca ttgactgaat ttctaagaac cagtttttca aatcttttta
     4321 ccctgttgcc gtagtgatct ctaaggtcat caaagaactc atagtcaaag gtacctgtat
     4381 gctcggtata ctctcgtctc gcaataagtt tcaatgattt tatgaatgca tttcttgaag
     4441 ttccgccggc ggttcgaatg ccacaatcct gggacagtac caaagttcca tccgattcaa
     4501 aaaagtctac atgggctttt ctttcccatt gtctgcctct ttggttgtag ttttttggat
     4561 accaccagtc ttcatctctc tgaggattat ttatctttgt gaaatcgtaa gtagcgccct
     4621 ttatcataat accttcttcg tatccgtaaa ggtttttttc gtcggtggtt atgcatacca
     4681 ccatggctcc aaagtgttct gcaaagtctt caatacctgt acctattatg tatgtatatg
     4741 tttgtgttct tccgtgcttt ttaccgtctg cgaaaatagc agccctaacc accacaaccc
     4801 ttgggaagtt attggaggta tttataacat cgatgctttt tgtgtacttg taagtttttc
     4861 gtatgcctgc aaagtccata tactcataat caggctcttt gccgccggag aacatgtaga
     4921 cttcgcctgt ctttgttatg agttcgcctt tctgcggagt ccttgcgttg tggttcgtca
     4981 ttgtgtagag aatctctaag tctttatcat ccacaacttc atcgtcttcg tttaatatgt
     5041 aaagtgacag ggagaggtca tggggataga atccaggatt gtaggaagga actatcctgt
     5101 atgtatgctc aggtttaact tcttccccgt tactaccagt tatgcttatt agaaatataa
     5161 ctgtaaggag cagaacaatc agggcgaata tggactttaa agctatttta tatgctctca
     5221 ctttttacaa aacctctatc aacatatttg caaacaagtt gtgcatttct cttgtgggat
     5281 gattgataag attactaagg agaactgttg tgtcgatacc ttcctcatgc atttgtttcc
     5341 attttgaata agcatcaaca acagtaacat tcattctttt gcacagggct tttgcagatt
     5401 caatatataa atcaaaagta ccgccattct gcatggcggc ggtagttttt gcagcttcct
     5461 ttgcagaatt aagagtttca tcatgaacgt atgtgttcat catgttcgga gtcatgaata
     5521 tgaccctaat acctttgtct tgtatttgag taaatatatg ttcaagtttg gatatataat
     5581 tatcaaggtt atctcttcct ctgcacacgt cgttcaggcc aaagcataca acgacagtat
     5641 caggattgtg ttcaagaaca tcttttgaga gtcttgctac tgcaccatcc acactgtcac
     5701 cgcttattcc ggaatttata acttcaagag gttcaacccc atatttttga gtccaaagct
     5761 tttttaattt gttgtggtaa acatttccat aatccctaac tgtgtcaaaa ccgccttcga
     5821 catcatatag ttcaaaacat ccttctgtca cactgtcgcc taaaaatact attctcatac
     5881 acaacctcat ttctttaaca tatgattatt gtaacatttt tattgtgttt tgcaacacac
     5941 attttactcg gtacaatttg tgaaatcgac ctccttttaa aattttttta cttgcaattt
     6001 caactgtttt gtgttaatat aatcccaatg cacttgtagc tcagtggata gagcaaccgc
     6061 ctcctaagcg gtaggtcgga cgttcgactc gtctcaagtg cgccatgagg gctgtcacgt
     6121 ttcgtgacag cccctcttgt tattcaagca atgttttaac catgtttttc tcccagtcaa
     6181 tatcaacgta taaccttgta tttctttttg attaatatct cttgcttgat acattttatg
     6241 catgtataat attaacaaat attgttaatc tggaggatgc gatgaaaaag aacacatagc
     6301 ttgttatact tgccgttgta ggtatatgcc ttttggtaat tgcatatgta cttcatttga
     6361 tggattacat accaggattg aaaacagtat tagatcgtgg tttgttcgat gaaaaatata
     6421 tgctttggat aggcaacact gaaatcatta tgatggcact ggccggcatg tttggattgt
     6481 tgtgtcttaa ggttaattct cttaggcatg tgagaaatgt ttttctgatt cttcttgtgt
     6541 tatttatgct tgcaagttca tcagttttgt ttttcttcac tgaagacaag gttcctcatg
     6601 gtggaaatgc gcttctgtct gaaaacatgc attatcttat gattcgctat gcagccatcg
     6661 gtggagtgct ggctattata ctgtatgtaa ttgctgtttt tgcaaaagac agaatattca
     6721 gtataattgc aacgatagtt ggcgtggctg gacttctgtt ttctcttatg gcgtttacgc
     6781 ttacaagaat aagtatcttt gaaaaccttg ttgagcaaag caagagctac aggtttgatt
     6841 cggtgcagta ttatccaatg ctactttttc atttaggctc tatattggtt attgtttcac
     6901 ttttgcactt gagtagagtc gcaataaaaa aaggagatga aacggagata ccttaattta
     6961 tgtggataat gatttttttc tgtattgtgc aggggtgcat ttcatttcct ttttaaaatg
     7021 tctgtaaaac agctccctgg taggatatcc tgtaagattt acaatatcgt ctatggggag
     7081 cattgtatta cgaagcaata gacatgcgtt gttgagtctg tactttgata ttatttcagt
     7141 cagagtcagt ccagtggttt cctttatttt tctactcatg tatgatggtg aatagttgaa
     7201 gagctttgat atggatttta gtgaagcatt tttgggattt agttttatat atccaagtat
     7261 gttgactatg tttatatcat tttcctttgt gcttgtatta tccgatctgt aaacttttat
     7321 atccaatgaa tgatttttga ttatgtacgc cagaatgata tggagaagac agtccttaac
     7381 ggatgatgac agttcattgg ggtatattgc tgatatataa gggtgtctaa aaaagagcgg
     7441 atctgtatgt tatctcctgc tggacagaat atgattccag gattattgtg ctcaacagta
     7501 tcgttaaaat agttggatat aatgttgtcg ctacaaagta tgctgtaaaa gagtttctta
     7561 aaatttgatt tccttacaat aatattcatt aaaacactgt cgctgtcaat ttcaattgaa
     7621 tgcttgacat ctggccccat tatgcaaaaa tcaccctcat acatttgaat tctgttccct
     7681 ccaagtatat taatgcaggt tcctctatag acatagggga tttcaacaaa ggaatgggag
     7741 tgcatacatg acggcatata ccttgcatgc ctaataacta cgatattgtc agatgttttt
     7801 tcaaatgctt cttcctctgt gtaatatgga gtatagtgtt gcatgttttc ataatacatg
     7861 aatctttcca gagggtagga aaacctggca caaacatctg gtatgtcttc agagcttttt
     7921 acaagtttct ttagctccat atattttaag ttatatagtt catcctcagt aaatactatg
     7981 gatgcatcta ttgctgcacg tatgtccata agacacctcc gagtaaagaa aagatacata
     8041 ttattatata attatacact gcgcaattta aattaattat atataattac aacaacctat
     8101 tcaatgttga taattataaa atgtttggag gagtagttta taagcttgtc cttcagtctt
     8161 tgcaacgatt aagacattgt aaatattgcg gaggtgccat atgtgtaaat cacgtgttct
     8221 gatactggtt gtatttatag cggctgtggc agtgttcctt ggtgcttgtg acagtgagtc
     8281 aaattctgac ggtaatgtta aacccacagt aaatcaaaca ccaaagatta cagcagctcc
     8341 tacagttacc aatactccat ctccaacgcc gaatattggg aataacgtag ctctgtacaa
     8401 gaaaactcag gtttcatcta cggtcgatga gagttatctc cagtggggat ggggtcagga
     8461 gtttattaat gacggaactt tggagttgag ttttacttct gcagtcaaga ggaacatgga
     8521 ttcccctgat gtagaagagt ggattataat cgacctggaa aaagagtatt caattcatct
     8581 ggttatactg tatccccgtc aggataatgg acgtggtttt cctgaggatt atcagataga
     8641 tgtgtcttcc gataacgaga catgggaaac tgtgtataca aaaactgaag acaaaggttc
     8701 tgaagacgag gatgtagatc ccagagaaat tgagcttgaa gcagtttcag caagatacat
     8761 aagattgctg gccacaaaac tcacggatgt tccagataca agtgatggat atctgctgca
     8821 gatggctgaa atggaggtat attcctttga tgaagagtaa agcgtactat tttacaaaga
     8881 aaggtttagt gttgttgatt atgttggctg tgatttgtag tgcaggctgt agctccagca
     8941 gtagagggga tttaaaaaag atatatgatg agtatgaagg caatacttta ttgataggcg
     9001 gttgggtaac gcccagagga gagtttgtaa aaactgctga agaggcagaa gcaagataca
     9061 aggaacttgc agatgcagga atcaacatga tgtacacttt tgacgataca gacgatcctg
     9121 atagattgaa aagagtcatg gacgcagcag aaaaaagtgg aatcaagctt gttgtccatt
     9181 tggacttaaa taattttaat cactcattgg atatagtaaa catgacaaag gatcatccgg
     9241 cagttatagg atacatcctg aaggatgaac cccctgcatc cttgttcccc gggctcttgg
     9301 ctttgaggaa aaaagtatac gatattgtag gggatgacaa aatcattatg ataaatttgt
     9361 tacccaacta tgctagtgac gcacagttgg gagtagtacc taaggatggt aaaacatctt
     9421 atcagatata tgtggagcag agtatgaaaa tcgttaaccc taccttgttg tgtactgatt
     9481 attacatgtt ccgtgccggc aaggatggtg acccaggggt gctggctgga tattatagaa
     9541 acatgtttga tttaagagat gcagcgaatg agtatgaagt agatctatgg gggtttctgc
     9601 agaatagcgg ctgggcaggc atgagggagc cgaacaaggc cgagcttcgt ttttctgctc
     9661 atgtaaatct ggctttcgga gtcaagtgcc ttgcgtactt tctgtgctgg aatccacatg
     9721 cggacagtat aaagggtgat ggcagatatg atcctaccag catgatatct tatgacggga
     9781 agataactga caactattac aaggttcaag ccataaacaa ggagctgcat gcaatgaaag
     9841 gtgtttatct tgattacaaa cataaaggca ttatcatgta tgaaaaaaca ccttttgtaa
     9901 aaagcacaat tcctgaggaa aaccttttgg agtcttttaa tgaactggtg agcttggatg
     9961 cagcaaggaa cacccttgtc ggatgctttg aaaaagaagg caaaacaggt ctatatgtgg
    10021 taaactgtga ttattctgat aatggagtca atactgtcaa gttgaagctt aatgacaagc
    10081 acacgtacag aatatggagc aaggatggat tgattgcaca gaagaaatcg gatacagtca
    10141 gtctcaagct tattccggga gaaggtgtgt ttgtacagat aattgattag aatcaggaag
    10201 cggtgcaaga tttgcaccgc ttatttttgt tcagtaatgg cattttgtct tatccgggtg
    10261 catatttgca actaatgatg aaatcttagg tgaattcata accatagccg ggccttctgg
    10321 aaaaggcaaa tcaactcttt taa
//
LOCUS       DAONVY010000013         9784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_5341_length_9784_cov_3.607976, whole genome shotgun sequence.
ACCESSION   DAONVY010000013 DAONVY010000000
VERSION     DAONVY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5341_length_9784_cov_3.607976"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLI11_00750"
     CDS             <1..988
                     /locus_tag="PLI11_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51426.1"
                     /translation="TGWNEWTAGKQTNPYPNMRTGHTSTPYYHFVDQFNPEFSRDAEP
                     MKVRDGVGFGDNYYYQMVNHIRLFKGIEPIKGVKDTNGISFDGIAEEGEKRLLQVDDK
                     IMSQWDKIEYRYMDSVGDTAFRNFNSMGSQYRYINATGRNDIDYAKVTQDDYFLYFMV
                     KTASGIISVDEENWMNLFVDIDKNHETGWEGYDFVINRSRDGKIVIVEKFVNNSWEFE
                     TVGRADYLMGSDNLTIKVSKELLGISENDFSFDFKWADNSTNIGNVMQFMDLGDCAPN
                     DRFNFRFTTTDIQYKPKALGQLNYVLITLGVVFVILIAIFIYKKVKVKGERK"
     gene            985..1836
                     /locus_tag="PLI11_00755"
     CDS             985..1836
                     /locus_tag="PLI11_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HPZ51427.1"
                     /translation="MRKHILLVMVLVLVLTIVSCNKQDKEPSATQTPQLTQEPTAVPT
                     EGPTAEPKATLKPIITNKGYPQEEAEVLIFEDFDDGEIDENEFVVVLGENADIIDGEM
                     HITREWPALSPEYDYAIGTLHHQYEFSMEYYSTFGRLSAPHVAYWVGARVPPERYAAY
                     PGGFWVAFTHTNIAFVYPGGDNFEGKEHWAEKCFQINVPEEFTTKHKITVVDTGDEIY
                     YYMNTTDEEYYLILKAVLDGADLLLYDKDDNMFWAGENALNDEIGFSIFSHRQASHTD
                     NVTLKGH"
     gene            complement(1878..1970)
                     /locus_tag="PLI11_00760"
     tRNA            complement(1878..1970)
                     /locus_tag="PLI11_00760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1932..1934),aa:Ser,seq:gga)
     gene            2230..2724
                     /locus_tag="PLI11_00765"
     CDS             2230..2724
                     /locus_tag="PLI11_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51428.1"
                     /translation="MKSKLVVLLVIVMIFSSLMVVNAETVLSLDNDEYVVGETVTVTF
                     SNATGNESAWIAIYPEGKDYTEYTAEEKAWKYANNNSDETPGETAATEGTVTISTEGL
                     APGEYRVSYFVDGAYNEGAFANFTLVEASQNPPTSDSTVFMTAFAMLLMAAVVLKHKS
                     VTSF"
     gene            2814..3719
                     /locus_tag="PLI11_00770"
     CDS             2814..3719
                     /locus_tag="PLI11_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51429.1"
                     /translation="MKKKLAFFILFIFLLSFVFVNAEENEYEHDDEYYEKMPYIWMTF
                     DYEGAAADMTSDGNPHGVELIDSKEGIIVKMAGEINDPLSDPYFYFPVSHDRLSDIFC
                     EEVQAIALVIKTDVAIKHPDAVNQPELFFRTVQDPTFARVGHPDWTDYFAGNETWAPG
                     TEGYKATDDWQVLVFDMSQNSYWTSIPDRLRLDLFDVCLDVEYVVKGIAFFLQPEHAL
                     EWDGDFDRVAFEEEYAKANQPTPAPTAEPTEAPEETEAPKQTESTTKDEKDIDNMTWL
                     YVVIPVAIIVVLVVVFIIVRKRKER"
     gene            3725..4672
                     /locus_tag="PLI11_00775"
     CDS             3725..4672
                     /locus_tag="PLI11_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPZ51430.1"
                     /translation="MRIKKALCLMLVLVLGFLMACSKGGQADPTPVPYEGNTTMYQLP
                     ESTPNRLQMMSYVIQTKDNKIIVLDGGNEGDTEYFLDFLKDLTKVDKPHVDHWFLSHA
                     HSDHTDVFTKVIEDDTVTISNVYHHFPPVEDVDRYERVEVHTIKRINAAFELIKDKTE
                     IHEVQVGDRITIGSVEVEVLFVYMPEIKSGFLNNSSTVFRFHVEGTSILFLGDLGYEA
                     GDILLQCYGDDLKSDIVQMAHHGSNGVRYNVYNKIAPKICLWPTPDWLWNNDQGKGYN
                     TGPWETITLHKYMIKLGVETHYVSKDGLISIVFPLDETN"
     gene            complement(4669..6639)
                     /locus_tag="PLI11_00780"
     CDS             complement(4669..6639)
                     /locus_tag="PLI11_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51431.1"
                     /translation="MSEIKYEVYTFDEPNIPYIITINQLGENYDKRETFLSWHENLEI
                     IHILSGTANVISDGESITACTNETVIINTDTLHKIIAASNNLSYMSIVVENEFLSGIF
                     HTDINSLWFNEHVPEEQLVSDIINRIINENKNKHLLYDSVIKSLIIELLVTLFRQHVT
                     YRSVKESSNKMIRQKDTLINALIYIRENFTKDISIDELSSLSGLSRSHFCTSFKELTS
                     MTAINYINTLRCKYAKSLLMTKKYTISEVSYMCGFNNISYFNKTFKKHYGITPSKLYE
                     STDTIKNKYSFIKIENKFGQWPEKIDKPYYKSKVGTTFATWWTEPTSPTHEHWYAKNF
                     NRLKPVEYDYYSCGDPEYLANVFERMKNIGIDFVLLDDNNPHWNDFGYIARNINICFE
                     TARSMKNNAPKLAILTGHPLIEGKMDVLQYSIDMYYYDYVSKFPETYYNWKDKPLLVL
                     NVAREIDKIGSYYDDRFTIRYGTGLVRQQKFIKDLETEKQEFVMSILSQTGNWARVFD
                     APNPNPEIIGVNPGYSKVHSGIDGYYIPRENGKTYMNMWLEAIKSNREVILIGSYNIH
                     PEETGWEAVKPIRKISTDNDDPCKDPFLYEKITEAYLALRYGFIEGFKYREESSTEVY
                     LYCENKLVKHSSNDNDLVIVIPDGYFDWKSKH"
     gene            6820..7770
                     /locus_tag="PLI11_00785"
     CDS             6820..7770
                     /locus_tag="PLI11_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51432.1"
                     /translation="MADIKYRWFNLDICIAKSDSYEDVVKDKDYLIENEIGLVVSVFN
                     HKFSMIQKCDADVLLRHYMFCVENWPQLSDWELKMLLSLVLYETDYGRKVAFWFGDDE
                     AEDAFFKAAGRPHEYAYVTPPDYINVCGACKQGKCITEYVCYTAGLEEAKQIFENVSI
                     KSLARAKNMSPEVLVKDPVNYQGDPPDYFQYVPFTWGNCFFGDRTVLEKILGRVIYEE
                     DLRNFFSPTVKFYFRYDDIAELNDAVLDGYHPVKVRGSVSLSNLLVACVIPQEHKDGL
                     RGFLSRDIKDRLVYADRSTCSTIWDWAETAYIAFVEFLDK"
     gene            7868..8677
                     /locus_tag="PLI11_00790"
     CDS             7868..8677
                     /locus_tag="PLI11_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51433.1"
                     /translation="MNCKNCGAFIKETDNFCETCERPISRIIPMKEKTRSSKNITILL
                     ILLIITFVSIGILLNISRIFGATDLGIEVNRKHYESAMKKFTASLKKHDGYTDIVLTN
                     EEITSLVKYNWPENDVVKDMQVRINKNSSIEAAGLINKEYIAQYLLTEGNGDSIYKVL
                     PMFDAMPKYTDVYAKFRCEIKNNRVNYIAIDKVEIMGIEVPESMYKNANTMYMLADGI
                     NQFIENVTDKTGASFDLVKAQSGKLIVKGLLPEVVYKEDLEMALETFSLKW"
     gene            complement(8719..9762)
                     /locus_tag="PLI11_00795"
     CDS             complement(8719..9762)
                     /locus_tag="PLI11_00795"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPZ51434.1"
                     /translation="MVEVQKFDTLHEEEINLYRISNKTVAYVEILNYGGIIRSICIPD
                     KNGMLVDVVIGFETINDYIKAESGYFGAIVGRCANRIKNASFTLNNKTYRLAKNDGNN
                     HLHGGYNGFDKRIWDVEILAEGNGIKLSLLSKDMDENYPGNLNVSVKYMFDDMNALTI
                     HYEAWSDADTILNLTNHAYFNLGGHNSGHIGQTFLKINADSYLPSDDELIPIGFIDKV
                     EGTVFDFRNYRKIYEGLESKDPQILLARGYDHNFVLDKSQGDFAASCYNEATGIRLDC
                     YTDMPGIQLYTGNFIDIDFYGKESYVYKRQEGFCLETQYFPNSINNPEWIQPILRKNE
                     KYDSTTRYIFSTI"
BASE COUNT         3011 a         1693 c         2091 g         2989 t
ORIGIN      
        1 tacgggatgg aatgaatgga ctgcaggcaa gcagaccaac ccttatccaa atatgagaac
       61 aggccatacc agcactccgt attaccattt tgttgaccag ttcaacccgg agttctcaag
      121 ggatgcagaa ccaatgaagg tgagagatgg tgtaggcttt ggagacaact actactatca
      181 aatggtaaac cacattagac tttttaaagg gatagaacca ataaaagggg taaaggatac
      241 gaatggcata agctttgatg gtattgctga agaaggagag aaacgtttac ttcaggttga
      301 tgataaaatt atgagccaat gggacaaaat agagtacagg tacatggatt cagtcggtga
      361 tacagctttt cgtaacttta actcaatggg ttcccaatac cgttatatca atgcaacggg
      421 aagaaatgat attgattacg caaaggtgac tcaggatgat tatttcttgt atttcatggt
      481 aaagacagca tcggggatta tcagtgtaga tgaggaaaac tggatgaacc tgttcgttga
      541 catagataaa aaccatgaaa ctgggtggga aggttatgac tttgtcataa acagaagtcg
      601 tgacggaaaa atcgttatag ttgagaagtt tgtgaacaat agttgggagt ttgaaactgt
      661 tggcagagct gattacctta tgggaagtga caacctgact attaaagttt caaaggaact
      721 gttaggtata tctgagaatg atttttcatt cgatttcaaa tgggctgaca actccacaaa
      781 cataggcaat gtaatgcaat tcatggattt gggcgactgt gcaccgaatg acagatttaa
      841 cttcagattc acaaccacgg acattcagta caaaccaaag gcattaggac aacttaacta
      901 tgtcctgata actttaggtg tagtatttgt catactaatt gcaatattta tatataaaaa
      961 agtaaaagta aaaggagaga gaaaatgaga aaacacattt tgctggtgat ggtattggtg
     1021 ttggtactca ccatagtgtc atgtaataag caggacaaag aaccaagtgc aacccagaca
     1081 cctcagctta cacaagaacc tacagccgtt cctacagaag ggccaacggc tgaacccaag
     1141 gcaactctta agccgatcat cacaaacaag gggtatcctc aagaggaggc ggaagtactc
     1201 atatttgaag attttgacga tggtgaaata gatgaaaatg aatttgtagt tgtactgggt
     1261 gagaatgcag acataatcga tggtgagatg catattacaa gagagtggcc tgcattgtct
     1321 cctgaatatg attacgccat tggaacactt caccatcaat atgagttctc aatggagtac
     1381 tactcaacat ttggcagatt aagcgcacct cacgtagcat actgggttgg ggcaagggtt
     1441 ccacctgaga ggtatgcagc atatcctggt ggattctggg tggcttttac acatacaaat
     1501 attgcctttg tttatcctgg tggtgataat tttgaaggta aagaacactg ggcagagaag
     1561 tgttttcaaa taaatgtgcc ggaggagttt acgacgaagc ataagattac tgttgttgat
     1621 accggtgatg aaatctatta ctacatgaac actaccgacg aagagtatta ccttatcctt
     1681 aaggcggtgc ttgatggtgc ggatctgctt ttgtatgata aggatgacaa tatgttctgg
     1741 gcaggagaaa atgcactgaa tgatgaaatc ggattctcca tattcagtca tcgtcaggca
     1801 tctcatacag ataatgttac acttaaggga cattaacaga aaagaggctt tgggatttac
     1861 tccaaagcct cgttttttgg cggaaagaga gggattcgaa ccctcggtgg ggtattagcc
     1921 cacacacgat ttccaatcgt gcgccttaag ccagactcag ccatctttcc acaatttttt
     1981 tgacaacaaa ataattatag tacgaaaaag aggatatgtc aatatccaaa acttaagaat
     2041 tggctaatta atttccatcc attcatacat tcgagaagaa caaggtgacg gttgagtaca
     2101 tgactgcagt tgcatttgag atagcacggt aaacaaaata caattttttc agacaatagt
     2161 gcaagtatat tttagtcgat tgtgataata ttagttataa ccaaatattc aagttgtagg
     2221 aggaaaacga tgaagtctaa attagttgta ttattagtga ttgtaatgat attcagttca
     2281 ttaatggttg ttaatgctga aacagtactc tcgctggaca atgacgagta cgttgttggt
     2341 gaaacagtta cagtaacatt tagcaatgca actggtaatg agtcggcatg gatagcaata
     2401 tatcccgaag gcaaggatta caccgaatac actgctgaag aaaaagcatg gaagtatgca
     2461 aacaacaatt cagatgaaac accaggagaa acagcagcta cagaaggtac agtaacaatc
     2521 agcactgaag gacttgctcc tggagagtat agagtttcat actttgtaga cggtgcgtat
     2581 aacgaaggtg cttttgcaaa cttcacattg gtagaagcat cacagaaccc acctacttcc
     2641 gacagtactg tattcatgac agcattcgca atgcttctga tggcagctgt tgtattgaag
     2701 cataagagcg ttacctcatt ttaaatgata gaatcaaacg ttttgttgag gactttgcgt
     2761 aatttacgca aagttctttg tacatataca taataatttc aggaggaatt taagtgaaga
     2821 agaaactagc ttttttcata ctctttatat tcttgttatc cttcgttttt gttaatgcag
     2881 aagaaaatga atatgagcat gatgatgaat attatgaaaa aatgccatac atttggatga
     2941 catttgatta cgaaggtgca gcagccgaca tgacttcaga tggtaatcct cacggtgtgg
     3001 aacttataga tagcaaggaa gggattattg ttaagatggc aggcgaaatc aatgatcctt
     3061 tatccgaccc atatttctac ttccctgtat cgcacgatag gttgtcagat attttctgcg
     3121 aagaagtaca ggcaatagcg ttggttatta agactgatgt tgcaatcaaa catcccgatg
     3181 cggtaaatca gcctgagttg ttctttagaa cggtccagga ccctacattt gcacgggttg
     3241 gccatcctga ctggacagac tatttcgcag gaaatgaaac atgggcaccc ggaacagaag
     3301 gatacaaggc aactgacgac tggcaggttc tagtatttga tatgagccag aatagctact
     3361 ggacctcaat accagataga ctccgacttg atttgtttga tgtttgtttg gatgttgagt
     3421 atgtagtgaa gggaatagcc ttctttttac agcccgaaca tgcattggag tgggatggag
     3481 attttgacag ggttgctttt gaagaggagt acgctaaggc aaatcagcct acaccagcac
     3541 caactgctga gccaactgaa gcgccagagg aaacagaagc tccaaaacag acagaatcaa
     3601 caacaaagga tgagaaggat atagacaata tgacatggct ttatgttgta attccagttg
     3661 ccataattgt tgttctggta gttgttttta ttattgtgcg taaaagaaaa gaaaggtaat
     3721 tagaatgaga attaaaaagg cattatgttt gatgcttgta cttgtgttgg gatttttgat
     3781 ggcttgctca aaaggcgggc aggccgatcc aacacctgta ccatatgaag ggaacactac
     3841 aatgtaccaa cttccagaaa gtacaccgaa tagattacaa atgatgtctt atgttataca
     3901 gacaaaagac aataaaatta tagtattgga cggaggaaac gaaggagaca ctgaatactt
     3961 cctcgatttc ctcaaggatt tgacaaaggt agataaacct catgttgatc actggtttct
     4021 ttcccacgcc cactcggatc atactgacgt ttttaccaaa gtgatagaag atgatacggt
     4081 caccattagc aacgtatacc atcattttcc acctgtggag gatgtggaca ggtatgaaag
     4141 agtagaggta catactatca agcgcattaa tgcagcattt gagctaatta aggataaaac
     4201 cgaaatacat gaggtacagg ttggtgacag gataaccatt ggtagtgttg aggttgaagt
     4261 gctttttgtt tacatgccgg aaataaagag cggttttctt aacaatagta gcaccgtgtt
     4321 taggttccat gttgagggaa catcaatcct tttccttggt gatctgggct acgaggctgg
     4381 tgacatactg cttcaatgtt acggagacga cctgaaaagc gacatcgtcc agatggcaca
     4441 tcatggttca aatggcgtaa ggtacaatgt gtacaacaag attgccccaa agatttgcct
     4501 gtggccaaca cctgattggc tttggaataa cgaccagggc aaaggttaca acaccggtcc
     4561 ttgggaaact attacattgc ataaatacat gataaaactg ggagtggaaa cccactatgt
     4621 ttcaaaagac gggctcattt ccatagtatt ccccttggat gaaaccaact agtgctttga
     4681 tttccagtca aagtaaccat caggaattac aattaccaaa tcattatcat tgcttgaatg
     4741 ctttaccagt ttattttcgc agtacagata aacttctgta ctgctttctt ccctatattt
     4801 aaacccttca ataaagccat atcttaatgc aagatatgct tcagttattt tttcatacag
     4861 aaatggatcc ttgcatgggt cgtcattgtc agtggaaatt tttcgaattg gttttactgc
     4921 ttcccatcct gtttcttcgg ggtggatatt gtatgaacct ataaggatga cctccctgtt
     4981 gctctttatt gcctcaagcc acatattcat ataggttttt ccgttttctc ttggaatata
     5041 gtagccgtcg atgccagaat gtactttgct gtagcctgga ttcacaccaa ttatttcagg
     5101 attagggttt ggcgcatcaa aaactcttgc ccagttgcct gtttgcgaca atatcgacat
     5161 tacaaactcc tgtttttcag tttccagatc ctttatgaac ttttgctgtc ttacaagacc
     5221 tgttccatac cttattgtaa atctgtcatc gtaatatgaa ccaatcttgt caatttcacg
     5281 ggccacattc aatacaagga ggggtttgtc cttccagttg taatacgtct ccggaaactt
     5341 gctcacataa tcataataat acatatcaat ggaatattgg agtacgtcca tttttccctc
     5401 aatcaaggga tgacctgtta atattgcaag tttgggagca ttgtttttca tactgcgtgc
     5461 tgtttcaaag catatattaa tatttcttgc aatatagcca aaatcgttcc agtggggatt
     5521 gttgtcatca agcaagacaa agtctatgcc aatatttttc atcctctcaa aaacattggc
     5581 caggtactct gggtctccac aggagtagta gtcgtactcg actggcttta gtctgttgaa
     5641 gttttttgcg taccagtgct catgagtagg tgatgttggc tctgtccacc aggttgcaaa
     5701 agtggtccca acttttgatt tataatatgg tttgtctatc ttttcaggcc attgaccaaa
     5761 tttgttttct atttttataa atgaatattt gtttttgatt gtatcggtac tctcatacag
     5821 ttttgatggt gttatgccat aatgcttctt gaaagtcttg ttaaaatatg aaatgttgtt
     5881 aaaaccacac atatatgata cttctgaaat tgtgtacttt tttgtcatca gaagagactt
     5941 tgcatacttg catcttaatg tgtttatata attaatggcg gtcatggagg tcagttcctt
     6001 gaatgaagtg cagaagtggc ttctgcttag tcctgaaaga ctgctcagtt cgtctatgga
     6061 tatatccttt gtgaagtttt ctcggatata tataagggcg tttataaggg tgtctttctg
     6121 ccttatcatt ttattgctgc tttcttttac acttctgtag gttacgtgtt gtctgaaaag
     6181 agttaccaaa agctctataa tcagtgattt aataactgaa tcataaagaa gatgtttgtt
     6241 tttgttttca ttaataatac gatttataat atctgacaca agttgttctt ctggaacatg
     6301 ttcgttgaac cacaaagagt ttatgtcagt gtggaaaata cctgataaaa attcattctc
     6361 cactactatg ctcatatagc ttaagttatt gcttgcagca ataatcttgt gaagcgtgtc
     6421 agtgttgata attacagttt cattagtgca tgcggttatg gactcgccat cggagattac
     6481 gttggcagtc cctgataaaa tatgtattat ctcaaggttt tcatgccagc tcaagaatgt
     6541 ttcccttttg tcgtaatttt cacccagttg gtttatagtt ataatatatg gaatgtttgg
     6601 ttcgtcaaaa gtataaacct catactttat ttctgacatt tgtgctgcac ctttctaaaa
     6661 tggttatact cgaaaactac catacaaatc gaattgtgtc aataactagc gcagggttgc
     6721 caatttttac atggaaatta ggtataaata atatggattg ataataaacc taggataaag
     6781 tataatttaa tggaaaagtc tgttgaagtg gggaatacaa tggctgacat aaaataccgc
     6841 tggtttaatc tcgatatctg tattgcaaaa agcgacagct atgaagatgt cgtaaaggac
     6901 aaggattatt taatcgaaaa cgaaatagga ttggtagtgt ctgtatttaa ccacaagttc
     6961 agtatgattc aaaaatgtga tgctgatgta ttactaagac actatatgtt ttgtgtagaa
     7021 aattggcctc agttaagtga ttgggagttg aaaatgcttc tgtcactggt tctatacgaa
     7081 accgattatg gaagaaaggt tgctttctgg tttggtgacg atgaagcaga agacgcattt
     7141 tttaaagcag ctggcagacc tcatgagtat gcttacgtaa cccctccgga ttatattaat
     7201 gtttgcggtg cttgcaagca gggtaaatgc ataaccgaat atgtatgcta cacggcaggt
     7261 ttggaagaag caaaacagat atttgaaaat gttagtatta agtctcttgc aagagcaaaa
     7321 aacatgagtc ctgaagtcct ggtaaaagac cccgtaaact atcaaggcga tccaccagat
     7381 tattttcaat acgttccgtt tacctggggc aactgctttt ttggtgacag gacagtgctg
     7441 gaaaaaatcc ttggcagagt tatatatgag gaagatttaa gaaacttctt tagtcctact
     7501 gtaaaattct actttaggta tgatgatata gctgaattga atgatgcggt attggatgga
     7561 tatcacccag tcaaggtgag gggcagcgtg agtctttcaa atctgttggt ggcttgcgtg
     7621 ataccccagg agcataaaga cggtttaagg ggatttctgt ctcgtgatat aaaagatcgt
     7681 cttgtttatg cagacagaag cacatgttca acaatatggg attgggcgga aactgcgtat
     7741 atcgcatttg tagagttttt ggataaataa atttatattt atttcctctt tttgtttgtt
     7801 ttgctgtatg gaacaggtga attttggcaa agataatagc aaaaaagaat aaagagaggg
     7861 atttaggatg aactgtaaga attgtggtgc atttatcaag gaaacagaca atttctgtga
     7921 aacatgcgaa agacctataa gcaggattat tccaatgaag gaaaagacaa gaagcagcaa
     7981 aaatataaca attttgctga ttttattgat tattactttt gtttccattg gaatcctgct
     8041 taatatttcg agaatatttg gagcaactga tcttggaatt gaggtaaatc gcaaacatta
     8101 tgaaagtgca atgaagaagt tcacagcatc tttaaaaaag catgacggtt atacggatat
     8161 tgtattgacc aacgaagaga ttacttccct tgtcaaatac aactggccgg aaaacgatgt
     8221 tgttaaagac atgcaggtaa gaattaataa aaactcatca attgaagcag caggattgat
     8281 taacaaggaa tacattgcac agtatttgct gacagaagga aacggggatt ctatttacaa
     8341 ggttctcccc atgttcgatg caatgccaaa gtatactgat gtgtatgcga aattcaggtg
     8401 cgagatcaag aacaacagag tgaactatat tgccatagat aaagttgaaa taatggggat
     8461 tgaagtgcct gaaagcatgt ataagaatgc aaataccatg tatatgctgg cagatggtat
     8521 aaatcaattc attgaaaatg tcactgataa gacaggtgcc agctttgatc tggtaaaagc
     8581 ccagtcgggc aagctgattg taaaagggct gcttcctgaa gttgtgtaca aggaagattt
     8641 ggaaatggcg cttgagactt tctcgttaaa gtggtaaaag aaaacctttg gtgaacgaaa
     8701 acaccaaagg tttttttatc atattgttga aaagatataa cgtgtagtgc tgtcgtattt
     8761 ttcatttttc cttagtatgg gctgaatcca ttctggattg tttatactgt ttggaaagta
     8821 ctgggtttca agacagaatc cttcctgacg tttgtataca taggattcct ttccgtaaaa
     8881 atcaatatca ataaagtttc ctgtatagag ctgtattcct ggcatgtctg tataacaatc
     8941 cagccttatg cctgttgctt cgttatagca ggaggcagca aaatcgccct ggcttttgtc
     9001 caaaacaaaa ttgtggtcgt atcctcttgc aagaagtatt tgaggatcct ttgattcaag
     9061 accttcatat atcttcctgt agttcctaaa atcaaataca gttccttcca ccttgtcaat
     9121 gaatccgata ggtatgagtt catcgtcaga gggcaggtaa ctgtctgcgt ttattttaag
     9181 gaacgtttga ccgatatgtc cgctgttgtg cccacccagg ttgaaatatg cgtggtttgt
     9241 aagattaagt attgtatctg catcggacca ggcctcataa tgaatagtga gtgcgttcat
     9301 gtcgtcaaac atatatttta ctgagacatt taaatttccc ggatagtttt catccatgtc
     9361 tttgctgaga aggctgagtt tgattccatt tccttctgca agaatctcga catcccaaat
     9421 tcttttatcg aatccgttgt atcctccatg cagatggttg ttgccgtcat ttttggccag
     9481 tctgtatgtt ttattgttta gtgtaaagga tgcatttttt attctgttgg cacatcttcc
     9541 gacaattgca ccgaaataac cgctttctgc cttgatataa tcgtttattg tctcaaatcc
     9601 gatgactaca tccaccagca tgccgttctt gtccggtatg catatggatc taataatgcc
     9661 tccataattc agtatttcga catatgcgac agttttattg gatattctgt atagattgat
     9721 ttcttcttcg tgtaatgtat caaacttctg cacttccacc atataaagaa aagctcctta
     9781 caaa
//
LOCUS       DAONVY010000014         9699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_5389_length_9699_cov_3.922957, whole genome shotgun sequence.
ACCESSION   DAONVY010000014 DAONVY010000000
VERSION     DAONVY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_9699_cov_3.922957"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLI11_00800"
     CDS             complement(<1..923)
                     /locus_tag="PLI11_00800"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPZ51435.1"
                     /translation="MEVAKKDLKNIETPPVSNSYEVIERIIENIEKCIVGKTEAVKLA
                     LITLISKGHLLIEDVPGVGKTSLISSLASSINATFKRISFTPDVLPSDITGFSVYNRK
                     TGEFEFRPGAVDCNFLLADEINRTSPKTQSSLLEAMQEGSVSVEGVTYKIEQPFMVMA
                     TQNPIEHLGTYPLPEAQLDRFFMKIQMGYPSNQDEAHIVKQQMNSHFIKQLKPVATKK
                     DILRIQEEVSNVHIEDSLALYIVGVANETRNNQDILLGVSPRGSIALGRASQAFAYLA
                     GRNYVLPEDVKSAAIPVLCHRIILNKEAKIRR"
     gene            complement(941..2284)
                     /locus_tag="PLI11_00805"
     CDS             complement(941..2284)
                     /locus_tag="PLI11_00805"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPZ51436.1"
                     /translation="MIRFDTSHALQFVKHNEIENQKEMVVSAHKALHEKTGAGSEFTG
                     WVDLPVNYDKVEFERIKKCSEKIREDSDILIVVGIGGSYLGARAAIEMLAGSFYNLQR
                     MKDKKGPMVLFAGNSISSAYMHELVEFIENYDVSINVISKSGTTTEPAIAFRILKEQM
                     EKKYGREEAARRIYATTDKQKGALRTLAEKEGYETFVIPDDIGGRYSVLTAVGLLPIA
                     VAGMYIDKIMEGARDACLVYLNPDLEKNPCYQYAAYRNALYKKGKVIELMVNYEPSFH
                     YFTEWWKQLYGESEGKDQKGIFPAGADFSTDLHSLGQYIQDGLRVLFETVLLVDKPRH
                     SFTIGHDTENLDGLNYISGMDMDEVNRYAAIGTILAHKDGGVPNLVISIPEINEYNFG
                     WLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEDLGKELKNKLN"
     gene            complement(2281..3504)
                     /locus_tag="PLI11_00810"
     CDS             complement(2281..3504)
                     /locus_tag="PLI11_00810"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPZ51437.1"
                     /translation="MVTDFKRIKMKNPLVEMDGDEMTRVIWEKIKDILLTPYIDLKTD
                     YYDLSIENRDKTEDLVTIDAANATKKYGVAVKCATITANAQRVEEFKLKKMWKSPNAT
                     IRAILDGTVFRAPILVDGISPLVKGWKKPIVIARHAYGDVYGGVETYVEKGSKAVLQI
                     TDKNGNSKYIDVHDFEDSNGIVQSIHNTDSSIESFAISCFEYALDNKLDLWFSSKDTI
                     SKTYDHRFKDIFNDIYEKRYKDKFEEAGIEYFYTLIDDAIARVMKSEGGFVWACKNYD
                     GDVFSDMLATAFGSLAMMTSVLVSPHGYYEYEAAHGTVTRHYHRYLQGEKTSTNPIAT
                     IFAWTGALYKRGELDGLEELKAFASKLEEAVLSTVGQGYMTGDLVSISELPEKTRLDL
                     EEFLYKVKENLEEML"
     gene            3794..4339
                     /locus_tag="PLI11_00815"
     CDS             3794..4339
                     /locus_tag="PLI11_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HPZ51438.1"
                     /translation="MNIKFNTKESKITEHGIETMENKINLRFNRLLSNMSEEELNFHV
                     KVVDKKFQYKVELTLPYMGYTLRAETYDKLSSIAALDKAMDIMERQIVKVKTKLSRVK
                     NQVPEFKGAAPATGEDAEPSNYEVVRVKSYEMKPISVQEAIMNMNLLGHTFYTFLNRE
                     TDKISTVYKRKDGDYGLIEPQ"
     gene            complement(4389..6140)
                     /locus_tag="PLI11_00820"
     CDS             complement(4389..6140)
                     /locus_tag="PLI11_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPZ51439.1"
                     /translation="MKQHIIKAKPVDNFKQLINQSVKLFGDKEAFIAKKKNGDAPEYR
                     HITYIELKNEIDSLGTALANLGLVDVPIALVGENRYEWCLGYLTVTCGGNVVVPIDKE
                     LKAEEIANLIKRSEAKAIIFSGKMTKVIEEFAKQSDIAEYLICMDDMEIDIEGKKCYT
                     ISQLIQEGQKLLDEGDTRYVDVEIDSNKVSVLLFTSGTTGIPKGVMLSQKNICFNIES
                     VTQCLEYDYRDSALSILPLHHTYECTCGFLVMMYNGCRVAFCDGIRYIAKNINEYKPS
                     IIMLVPLILEGVHSKILKKAREKTAKYLAFKALLFISGVLYSLGIDIRKKFFKTVHES
                     LGGNLRLVIAGAAAMSPKISHDIQRMGINVRQGYGLTECSPIVCVNRETDVNNASVGP
                     AMPGVEIKINNPNEDGVGEILVKGKNVMLGYYKDPEATAEVLRDGWFYTGDLGRMDKK
                     GRLYICGRIKNIIVTKTGKNIYPEELEEKIKHIPYVLESIVWGYDNKDDGDTEVHATV
                     VPNYEAIKEAGFDDTTPDAVKALIWNEIKKVNKTVPVHKAIKEVHIREEEFEKTTTRK
                     IKRYVEKFIKSDKGNVG"
     gene            6318..6788
                     /locus_tag="PLI11_00825"
     CDS             6318..6788
                     /locus_tag="PLI11_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51440.1"
                     /translation="MDFKGILNFVKRNRKLLLFLILAFIEIFDLFCLSLIANNRFTTF
                     SESLLGKLRFNTLTPRPVIYDDMLRFTTYVLSFVVGGTLVFVVVKLTGKLAKGKELIN
                     YEALSLIPLALFVIIFLSGSFATNFSTRWLNPLLTYVGGVFLYFLSCIKMKVNY"
     gene            complement(6785..7426)
                     /locus_tag="PLI11_00830"
     CDS             complement(6785..7426)
                     /locus_tag="PLI11_00830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPZ51441.1"
                     /translation="MTKIKLAVFDFDGVLVDSAKDIARSVKATQENFNYKVMDEDEII
                     TYVGFGAKYLIDKTLPGSTEKELEWYTNYYYEHAVVDTTLYPGVKKFLQHISDKGGAM
                     CVLSNKPEPLVKKILKILEVDSYFNTVLGPESLKRMKPDPEGIIICMERNNAKSCETI
                     MIGDTYTDIRTGKACGAHTCGVLYGIGDREKLLEEKADIYVNDLNDILKEYEF"
     gene            complement(7783..8130)
                     /gene="rsfS"
                     /locus_tag="PLI11_00835"
     CDS             complement(7783..8130)
                     /gene="rsfS"
                     /locus_tag="PLI11_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPZ51442.1"
                     /translation="MDSKQLAEIIKDALDNRKGKEIVVIDTSGKTIITDYFIIASGTS
                     TTHLKGLADEVIFKLKEEGRECSHIEGYDTARWILLDYLDVVVHLFLEEEREYYNLER
                     LWKDGARPSNQDI"
     gene            complement(8130..9335)
                     /gene="nadD"
                     /locus_tag="PLI11_00840"
     CDS             complement(8130..9335)
                     /gene="nadD"
                     /locus_tag="PLI11_00840"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPZ51443.1"
                     /translation="MIRVGISGGTFDPIHIGHVKSAEYVYKTLELDEVVFIPNGQPPH
                     KVTSFVTDERHRFEMVKLAIKDYPAFSVSDIEMKKDGYSYTVDTLRALTKEYRQKFGD
                     VKLYYIIGTDVLGEITSWKESNEVFKLCEFVVLRRPGFVLDNAREKAEAGGAKIHVVD
                     APQIDISSTRIRELLEEGKDVKMYIPEKVYTYILSNNLYRERKAMGFEEIKKDLEGRL
                     SEKRYIHTLGVVDECERLGKIYGVDQENCKLAGLLHDCAKDMDIEQYKWLGVSTDATE
                     EELVNGFNVDVLHARAGRIIAAQRYGITDTEVLDAIENHVTGRPGMTLLEQIVFLADY
                     TEVNRRGVVYDKIRNELDKGLVNGIIAACDNTIKYLLDKNVLIRIETIQTRNYYLTEL
                     KKKSEREVT"
     gene            complement(9403..>9699)
                     /locus_tag="PLI11_00845"
     CDS             complement(9403..>9699)
                     /locus_tag="PLI11_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51444.1"
                     /translation="RKKELTDADLQKRSVKLVVMHLKKKVDPKVPGIQNVIEWIDKMD
                     EVLAKDEFDRAEYLEMRRQLYNAIEWVIDVDVRASLRRSWYSFGKAMDKKVPKK"
BASE COUNT         2492 a         2226 c         1661 g         3320 t
ORIGIN      
        1 cgccttattt ttgcctcctt gttcagaatt atcctgtggc aaagaaccgg aattgcagca
       61 ctctttacgt cttcaggcag cacatagttt cttccagcca aatacgcaaa cgcctgggat
      121 gcacgtccta gtgcaatact tcctctcggg cttacaccaa gcaatatgtc ctggttgttt
      181 cttgtttcat tcgctactcc aactatgtac aatgccagac tgtcttctat atgtacattg
      241 ctaacttctt cctgtattct caggatgtcc ttctttgtgg caacaggttt caactgtttt
      301 ataaaatggg aattcatctg ctgttttaca atgtgtgctt catcctggtt tgagggatat
      361 cccatttgta ttttcataaa gaacctgtcc agctgagctt ctggcagcgg gtaggttccc
      421 aaatgctcta tggggttctg ggttgccatt accatgaacg gctgctctat tttatatgta
      481 acgccttcca ctgaaacgct tccttcctgc atcgcttcca gaaggcttga ttgtgtcttt
      541 ggtgatgttc tgttaatttc atccgccaaa aggaagttgc agtcaaccgc ccccggcctg
      601 aattcaaact ctccggtctt tctgttgtac actgaaaaac ctgttatgtc ggacggtagt
      661 acatccggcg tgaaggatat gcgcttgaat gtagcattta tgctggatgc aagggaagat
      721 atgaggcttg tctttccaac acccggcacg tcctcaatca atagatgccc cttgcttatc
      781 agagttatta acgcaagttt gactgcttca gtcttgccga caatgcactt ctcgatgttc
      841 tcaatgatcc tttcaattac ctcgtatgag tttgagactg ggggcgtttc aatgtttttc
      901 aaatccttct ttgcaacttc cacttttttc actctccaaa tcaattaagt ttatttttaa
      961 gctctttacc caggtcttca tagccgggtt ttcctaatag agcaaacatg ttctttttgt
     1021 aagcttcaac tccgggttgg tcaaaaggat tcactcccaa caggtaacca cttataccgc
     1081 acgccttttc aaagaagtat acaagccatc caaagttgta ttcattaatc tcaggtatgg
     1141 agattaccaa attgggcact ccaccgtcct tatgggcgag gattgttcct attgctgcgt
     1201 acctgtttac ctcatccata tccattcctg aaatatagtt gagtccatcc agattctcag
     1261 tatcatgccc tatcgtaaag gaatgtcttg gcttgtccac aaggagtact gtttcgaaca
     1321 ataccctcaa tccatcctga atatactggc caagcgaatg caggtctgtg ctgaagtcag
     1381 cgcctgcagg aaaaattccc ttttggtctt ttccctcgct ctcaccatat aattgcttcc
     1441 accattcggt aaaatagtgg aaggacggct cgtaattcac catcagctct ataaccttgc
     1501 cctttttgta aagcgcattt ctatatgcag catactggta gcaaggattt ttttccagat
     1561 cgggattaag gtacaccaaa cacgcatccc ttgcgccttc catgatcttg tctatataca
     1621 ttcctgcgac ggcaatgggc aaaagcccta ccgctgttaa aacggaatac ctgccgccta
     1681 tgtcatcggg gataacaaag gtttcgtatc cttctttctc tgcaagagtt cttaatgcac
     1741 ctttttgttt gtcagttgtt gcataaattc tccttgcagc ttcttctctg ccatacttct
     1801 tttccatttg ctccttcagt attctgaagg caatagcagg ttctgtggtt gttccggatt
     1861 ttgatatcac atttatcgaa acatcataat tttcaataaa ttctacaagt tcatgcatgt
     1921 acgcagagct tatgctgttt cctgcaaaaa ggaccatagg tcccttttta tccttcattc
     1981 tctgcaagtt gtaaaaagag cctgcaagca tctcgatggc agctcttgct ccaagatacg
     2041 atccacctat tccaactaca attaatatat cggaatcttc ccttattttc tcactgcatt
     2101 tttttattct ttcaaactct actttatcat aatttacagg aaggtcaacc catcctgtaa
     2161 attcactccc cgcccctgtc ttctcatgaa gtgctttatg tgcggataca accatttcct
     2221 tttggttttc gatctcattg tgtttaacaa actgcagcgc atgggatgtg tcaaatctta
     2281 tcataacatc tcctctaagt tctctttcac tttatacaga aattcctcca gatcaagcct
     2341 ggtcttctca ggtagttcgg atattgaaac aaggtctccg gtcatgtacc cctgtcctac
     2401 tgtacttaaa actgcctctt ccagcttgct tgcaaaggct tttagttctt caagtccgtc
     2461 gagttctccc ctcttgtaaa gggcaccggt ccatgcaaat attgttgcaa tggggtttgt
     2521 tgaggttttt tcaccctgca ggtacctgtg atagtgtctt gttacagttc cgtgagccgc
     2581 ctcgtactcg tagtagccat ggggcgacac cagcacagat gtcatcattg ccaggcttcc
     2641 aaatgctgtt gccaacatgt cggagaaaac gtctccatca tagtttttgc atgcccatac
     2701 aaatccacct tcggatttca ttaccctggc tattgcatcg tcaataaggg tgtagaaata
     2761 ttctatgccg gcttcctcaa acttatcctt gtatcttttc tcatatatgt cattaaatat
     2821 gtccttgaac ctgtgatcat atgtttttga tattgtgtcc ttggatgaaa accacaaatc
     2881 caatttatta tccaaggcat actcaaagca ggaaatcgca aaactttcaa tagaactgtc
     2941 cgtattgtgg atgctttgca caatgccgtt tgaatcttca aaatcatgca catctatata
     3001 cttgctgttt ccgtttttgt cggttatctg caaaactgcc ttgctgccct tttccacgta
     3061 cgtctcaacg cctccatata catcgccata agcatgacgg gcaattacta ttggcttttt
     3121 ccatcccttt accagaggac ttattccatc caccagtatt ggtgctctga ataccgttcc
     3181 gtcaagtata gcccttattg tagcattggg gcttttccac atttttttaa gtttgaactc
     3241 ttctaccctt tgagcgtttg cagtaatggt tgcgcattta actgcgacac cgtatttctt
     3301 cgttgcgttt gcagcatcaa tagtgaccag atcttcggtc ttgtccctgt tttctatact
     3361 taaatcatag taatctgttt tcaagtcaat atatggagtt aaaaggatat cctttatctt
     3421 ttcccatata accctggtca tctcgtctcc atccatctca actaatggat ttttcatctt
     3481 tattctttta aaatccgtca ccatatcaac tcccatgtta ttagttttac cagattataa
     3541 tatcaaaaat gtctatgttt tacaagctga aaacaggaca tggcacaatc tatgataacc
     3601 acataatgtt attgtatgat agcaatattc atcaaatatc gagtgaatat atttttcttg
     3661 attaatgtaa atattttgtg tataatataa ataactttag gatagggagt gatgtataat
     3721 atgtagaata atccagctta aattttttgt tgggcataat cttgcactat tgttttagaa
     3781 ggaggaacct aatatgaaca tcaagtttaa cacaaaagaa tcaaaaatta ctgaacacgg
     3841 cattgaaacc atggaaaaca aaatcaacct caggttcaac agacttcttt cgaatatgtc
     3901 tgaagaggag ttgaatttcc atgttaaagt tgtcgacaag aaattccaat acaaggttga
     3961 gttgacgtta ccttatatgg gctacacgtt gagagcggaa acctacgaca agttaagctc
     4021 tattgctgct cttgacaagg caatggatat tatggaacgt cagatagtaa aggtaaagac
     4081 aaagctttca cgtgtaaaaa atcaggttcc ggaatttaag ggtgcggctc ctgccactgg
     4141 ggaagatgcc gagccttcaa attacgaagt tgtcagggtt aaaagttatg aaatgaagcc
     4201 catttctgtt caggaagcca tcatgaatat gaatttgctt gggcacactt tctatacttt
     4261 tttgaataga gaaacagaca aaatatctac agtttacaaa agaaaagacg gagattacgg
     4321 ccttattgaa cctcaataag gttttacata aaaaataaaa gctgcagaca tggtctgcag
     4381 ctttcttttt atcccacatt tcctttgtca ctttttataa acttttcaac gtatctcttg
     4441 atctttcttg tagttgtctt ttcaaattcc tcttccctta tgtgaacttc cttgatagcc
     4501 ttgtgaacag gtactgtctt gttcaccttt ttgatttcat tccagatgag agcttttaca
     4561 gcatcagggg tggtatcatc aaagcctgct tccttaatgg cttcataatt gggaactacg
     4621 gttgcatgta cttcggtgtc tccgtcgtcc ttgttgtcgt atccccaaac tatgctttca
     4681 aggacataag gaatgtgttt gattttttcc tcaagttctt ccggatagat gttctttccg
     4741 gtcttggtaa caattatgtt ctttatgcgc ccgcagatgt acaaccttcc ttttttgtcc
     4801 attctgccca ggtcacctgt gtagaaccat ccgtccctta atacttcggc tgttgcttca
     4861 ggatccttgt aatatccaag cattacattc tttcctttta caagtatctc gcctactccg
     4921 tcctcgttag gattgtttat ctttatttca acgccaggca ttgcaggacc aacggaagca
     4981 ttgtttacat ccgtctctct gttaacacag actattggcg aacattccgt aagaccgtat
     5041 ccctgacgca catttatacc catgcgctga atgtcgtggg atatcttcgg actcattgcc
     5101 gcagcacctg cgattacaag tctcaggttt cctccaagag attcgtgcac tgtcttgaaa
     5161 aactttttcc taatatctat acccagactg tacaaaacgc ccgatatgaa gagaagggct
     5221 ttaaaggcaa ggtacttcgc cgttttctct ctggcctttt tcaatatttt tgaatgaacg
     5281 ccttcaagaa taagaggcac aagcattatt atgctgggtt tgtactcatt tatgttcttc
     5341 gcaatatatc ttattccgtc acagaaagct acacggcaac cgttatacat cattacgagg
     5401 aatccgcatg tacattcgta tgtatgatgc aaaggcaata tggaaagagc cgaatccctg
     5461 tagtcatatt ccagacactg tgtaacagat tctatgttga agcatatgtt cttttgagaa
     5521 agcattactc cttttggtat tcctgttgtt cctgatgtga acagaagaac actgacttta
     5581 ttgctatcta tttccacatc gacgtatcta gtgtcgcctt cgtccagcaa cttttgtcct
     5641 tcctgaatca gctgtgatat ggtgtaacat tttttgcctt caatatctat ctccatatca
     5701 tccatgcata tgagatattc tgcaatatcc gactgctttg cgaattcttc aattaccttc
     5761 gtcatttttc ctgaaaagat tatggctttt gcctcggatc tttttatcag gtttgcaatt
     5821 tcctctgctt taagctcctt gtctattgga actacgacgt ttccaccgca agttactgtc
     5881 agataaccca gacaccattc atatcggttc tcacctacca gggcaatagg tacatccact
     5941 agtcctagat ttgcaagagc ggtgcccaag ctgtcaattt catttttaag ttctatgtat
     6001 gtaatatgcc tgtactcagg tgcgtctcca ttttttttct ttgctatgaa agcttctttg
     6061 tctccaaaaa gcttcacgct ctgatttatc aattgcttaa agttatcaac cggttttgct
     6121 tttattatgt gttgtttcaa aacactcaac cctttcgaat aaatgtttct ttgtattata
     6181 aaccaagcag tttaaaatta caatattaaa aatttgttat atccttgaat ataaataagc
     6241 ttttaaaagc aattcaccta tagtataata ctatagccac tatatattac gcaaaattaa
     6301 aacaggagtg aacacttttg gattttaaag ggattttgaa ctttgttaag agaaacagaa
     6361 agcttttact ttttttaatt ctggccttta ttgaaatatt tgacttgttt tgcttgagtc
     6421 ttattgcaaa taatagattt accacttttt cggaatcact tttgggaaaa ttacgattca
     6481 acacacttac acctagacct gtaatatatg acgacatgct gagatttact acatacgtac
     6541 tatccttcgt ggtaggtgga actcttgtct ttgtcgtggt caagctgacc ggaaaactag
     6601 ccaaaggcaa agaacttata aactacgagg cattgtccct gattcctttg gctctgttcg
     6661 tcatcatatt tttgtcaggt tcttttgcaa cgaattttag caccagatgg ctgaatccac
     6721 tgctgacata cgttggcgga gtttttcttt actttctttc atgtattaaa atgaaagtta
     6781 attattaaaa ctcgtattct ttcagtatat cattcaggtc atttacataa atatctgctt
     6841 tttcttccaa aagcttttcc ctgtctccaa ttccatacaa gactccacaa gtgtgagctc
     6901 cgcatgcctt tcctgtgcgt atatcggtgt atgtatcacc tatcatgata gtttcacagc
     6961 tttttgcatt gttcctttcc atgcagatga taataccttc cggatcaggc ttcatccttt
     7021 tcagagattc agggcccaat accgtgttga agtagctgtc cacttccagt attttaagta
     7081 ttttcttcac caaaggctcg ggcttgtttg atagaacaca catggctccg cctttgtcac
     7141 ttatgtgttg tagaaacttt tttactcccg gataaagagt ggtatcaacg acagcgtgtt
     7201 cgtaatagta gttcgtgtac cattcaagtt ctttttcagt tgaaccgggg agggtcttat
     7261 ctatgagata ttttgctccg aatccaacgt atgttattat ttcatcttca tccatcacct
     7321 tgtaattaaa gttctcttgt gttgctttta cgcttcttgc aatatctttt gcagaatcaa
     7381 ccaatactcc gtcaaaatca aatactgcca gtttaatttt tgtcatctgt taatcctttc
     7441 ctccatctaa aacattttat gctgctccca caatatccct tttgttcgtc atttattccg
     7501 ggagctttaa cgatgcaaat ttcaatttaa ccaatttgtt tatggtattc cagataaaaa
     7561 gttgcatttt gatacaattt gaacatcttg actttaatca tgaaaatcct ctcccactaa
     7621 atttctatgg gcaggatgag tatatgaagg tgtcaatggt agcatccgtt agattctggc
     7681 agacaccagc atgccaatcc ggcagagtca tcaactggtc ctccgttgaa aatcagtatt
     7741 tcgaacacag acccatttgg gtatgttatt ctattcaacc atctatatat cctgattaga
     7801 aggccttgct ccatctttcc acaaacgttc cagattgtaa tattcccttt cctcctctag
     7861 aaacagatgc actacaacat cgaggtaatc aagcaatatc catctggcag tgtcataacc
     7921 ttctatatgg gagcattccc ttccttcttc ctttagtttg aaaattacct catctgcaag
     7981 tcccttcaga tgtgttgtcg acgttccgct tgctatgata aagtagtcag taatgattgt
     8041 ctttcccgat gtgtcgatga caactatttc ctttcccttg cggttgtcaa gcgcatcctt
     8101 tattatttca gccagttgtt ttgaatccat caagtcactt ccctttcgct ttttttcttt
     8161 aattctgtta gataatagtt ccttgtttgt attgtttcta tgcgtatcag tacattcttg
     8221 tccaagaggt actttattgt attatcgcat gcagctatta taccattaac cagtcccttg
     8281 tccaattcat tcctaatctt atcatatacg actcctcgtc tgtttacttc agtatagtca
     8341 gcaagaaaca ctatctgctc tagaagtgtc attcctggtc tgccggttac atggttttct
     8401 atggcatcca acacctctgt atcagttata ccatatcttt gcgctgcaat aatacggcct
     8461 gctctggcat gtagaacatc aacattgaat ccgttgacga gctcctcttc cgtagcgtct
     8521 gtggaaactc ccagccactt gtactgctca atatccatat ccttagcaca gtcatgcaac
     8581 agtcctgcaa gtttgcaatt ttcctggtcc accccatata ttttccccaa acgctcgcat
     8641 tcatccacta ctcccagagt atggatgtat ctcttttcac ttaatcttcc ctcgaggtct
     8701 ttcttaatct cctcaaaacc cattgccttt ctctccctgt agagattgtt cgataaaatg
     8761 taagtatata ccttttcagg gatatacatc tttacatcct ttccctcttc aagcaactcc
     8821 cttatcctgg tggaagatat gtcaatttgg ggtgcgtcca ccacatgaat ttttgctcca
     8881 cccgcttccg ctttctccct tgcattatca aggacaaatc cgggacgtct taacaccaca
     8941 aactcgcaga gcttgaaaac ttcattggat tccttccaag aggttatctc accaagaacg
     9001 tcggttccaa tgatgtaata aagtttgaca tctccaaatt tttgcctgta ttctttcgtt
     9061 aaagccctca gggtatccac ggtgtagcta tacccatcct tcttcatttc aatatcggac
     9121 acggaaaagg cggggtaatc cttaatagcc agtttcacca tctcaaaccg gtgtctttca
     9181 tccgtaacaa aggacgtgac cttgtgggga ggttgtccat tcggaatgaa tacaacctcg
     9241 tccagttcaa gtgttttata tacgtattct gcgcttttaa catgtccaat atgaattgga
     9301 tcaaatgttc ctccacttat tcctactctt atcatactgt ctccttacga aaaaaagcat
     9361 ccattaaatg gatgcttgat ttagtacgtg agcttgttat acctattttt ttggtacctt
     9421 tttatccatg gcttttccaa aactgtacca tgaacgtctt aaactcgctc ttacatcaac
     9481 atcaataacc cattcaattg cattgtacaa ctgtcgtctc atctccaggt attccgccct
     9541 gtcaaactcg tcttttgcaa gcacttcatc cattttgtcg atccattcaa ttacgttttg
     9601 aatgcccggc acttttggat ctactttctt tttcaaatgc ataacgacaa gcttaactga
     9661 tcttttttgt aggtctgcat ctgtaagctc tttttttct
//
LOCUS       DAONVY010000015         9674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_5407_length_9674_cov_3.573137, whole genome shotgun sequence.
ACCESSION   DAONVY010000015 DAONVY010000000
VERSION     DAONVY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_9674_cov_3.573137"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PLI11_00850"
     CDS             complement(<1..1362)
                     /locus_tag="PLI11_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPZ51445.1"
                     /translation="MPKSLFIDPKEVRKSDWIEFGKIPVNQYNKSIEEEKENYTTDDF
                     LRIYTHMKIINEFEGMINSIKTTGEYNGIAYNHPGPAHLSIGQEASAVGQAYLLTVDD
                     FIFGSHRSHGEILAKGLSAIHQLDDNKLMEIMESYFDGAILKIVEKDQKSVKELATDF
                     LIYGTLAEIFARDTGFHRGLGGSMHAFFTPFGIYPNNAIVGGSATIATGCALFKKVNR
                     KNGIVIANIGDASLGRGPVWEAMCFATMDQFTQLWEGDMNGGLPIIFNFFNNQYGMGG
                     QTSGETMGQGLLARFGAGVNPDMMHAERVDGYNPLAVIDAIRRKKRIIEEKRGPVLLD
                     TLTYRVSGHSPSDASSYRSKDEIDAWIEANAIDEYKNKLIDAKVATEDQFNEISEQVT
                     TLITKLTAMSVDEKVSPRMDLVKNPRAIEDLMFSNEHVEKMEDREPEVLMPLEENPRV
                     KQLK"
     gene            complement(1383..2747)
                     /gene="lpdA"
                     /locus_tag="PLI11_00855"
     CDS             complement(1383..2747)
                     /gene="lpdA"
                     /locus_tag="PLI11_00855"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPZ51446.1"
                     /translation="MVYDLIVIGGGPAGYLAAERAGKEGFKVLLVEKRFIGGVCLNEG
                     CIPSKALLYSAKLYDGVANGEKYGVTAKELKLDHDYVIKRKNKVVRTLVGGINSSLKK
                     NKVTVVMKEGTITGRNADGFTVKAGDDVYTGKKLLIATGSVPVVPPIPGVKEGLESGF
                     VLTNREILDLTEVPKELCIVGGGVIGLEMASYFNSAGSNVTVVEMMNKIAGPMDREIS
                     EILLKNYQKKGITFNLGSRVTEVKAGMVIYEKDGKSYEVPCDKVLLSIGRRAFTEGLG
                     LETIGVEVERGRIITDERSRTNIAGVYAAGDVNGKSMLAHTAYRETEVALNDILGKKD
                     IMRYNAIPSVIYTNPEVAAVGETEETAKAKGIDFEVVKLSMMYSGRYVAENEGGDGII
                     KILIDKKYRNVIGVHMIGNYSSEIIYGAGIMIETQMRIDDIKEIVFPHPTVCEVIREG
                     IFEF"
     gene            complement(2762..4105)
                     /locus_tag="PLI11_00860"
     CDS             complement(2762..4105)
                     /locus_tag="PLI11_00860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPZ51447.1"
                     /translation="MPRQGNTVESCIISQWHKKVGDKVNVGDILFTYETDKATFDEEA
                     KVEGTLLAIFFEEGDDVPVLMNVAAIGEEGDDYSDFIPGGVAEVEEEVAAPAPAAAPQ
                     APVKQVAAEEKASVTDTGRIMISPRARNLAEKAGVNPMFVSGKGPYGRIIESDIKDFI
                     AQGGATTYAAMDKATKDMAGTGIGGRVTTSDLTAQPAPQAQVAASTPAPVKEAAPFVE
                     YEDVKLPNIRKVIAKAMHHSLSSMAQLTLNASFDATNIMEFRKQVKANKDKLGLENIT
                     INDIILYAVSRTVLNHRDCNAHFLDDTMRYFNTVNLGVAVDTPRGLMVPTLFNAERKS
                     LNEISKEAKSIAQMAQSGNINPDLLQGGTITITNLGTLGIESFTPVINPPQTCILGVN
                     TLETRVKNVNGEMKMYQAMTLSLTFDHRALDGAPAARFLQELCRNLENFSILLAK"
     gene            complement(4280..4468)
                     /locus_tag="PLI11_00865"
                     /pseudo
     CDS             complement(4280..4468)
                     /locus_tag="PLI11_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
     gene            4729..4995
                     /locus_tag="PLI11_00870"
     CDS             4729..4995
                     /locus_tag="PLI11_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51448.1"
                     /translation="MKFLNIRPKLRLVFLASYLATAVWIMVKKFSFIYIVAGLLFLFG
                     CYISLVKAEVIKDSRADNIDNFSFDFVSFIIILVLVFDIVFSVL"
     gene            5092..5334
                     /locus_tag="PLI11_00875"
     CDS             5092..5334
                     /locus_tag="PLI11_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPZ51449.1"
                     /translation="MKTYMIMLKSIDDVKRFVNAATTLTCEADLSSGRYIVDAKSIMG
                     IFSLDLSRPIKLELHCNEDHCCDGFVDKIKDFIVEE"
     gene            5413..6711
                     /gene="mtaB"
                     /locus_tag="PLI11_00880"
     CDS             5413..6711
                     /gene="mtaB"
                     /locus_tag="PLI11_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPZ51450.1"
                     /translation="MKVATYTIGCKVNQYETEAVLEMFLNKGYTVVPFSEKADVYIIN
                     TCSVTQMSDAKNRQMIRKCHRLNPNAVVAVMGCYSQVAPDEVAQIEGVDIVIGTQNRS
                     KLLEMAEELLKNKKEANEPTVDVNATDFRVELPFEELSISRFEGHTRAFIKIQDGCSQ
                     FCSYCIIPYARGPVRSRTLESLRNEVEKVANMGFLEVVLVGIHLASYGKDLEPRVTLK
                     DAVSAASSVEKIKRVRLGSLDPMYLSDDVIKALSENQKFCRHFHLSLQSGSDSVLKRM
                     NRKYTTDEYMEIVDTIRKYMPDCAITTDVMVGFPGETEEEFLESLEFVRSVGFSRIHV
                     FPFSPRKGTVAYKMKDQIENSVKKERVDRMIALGMDLEQRFLGSMTGKVLDVLCEKDC
                     VGYTGNYVKVVVNEEDFKEGELILVRITGVTGNECIGERI"
     gene            6787..7041
                     /locus_tag="PLI11_00885"
     CDS             6787..7041
                     /locus_tag="PLI11_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HPZ51451.1"
                     /translation="MDNKTVFYKREKEQQNETRVVLTEVYEALKEKGYNPISQLVGYI
                     MSGDPTYITNHKNARALVTRVERDEIVEELFKYYLETPQD"
     gene            7051..7491
                     /gene="ruvX"
                     /locus_tag="PLI11_00890"
     CDS             7051..7491
                     /gene="ruvX"
                     /locus_tag="PLI11_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPZ51452.1"
                     /translation="MRVLGIDYGDRRIGVSVSDAFGWTASSLGFIDVKKYKNEVFDII
                     GQYIKEYQTETIVVGYPLNMNGTKGPRAEITDLFITQLTEKFPDIKTVKWDERLTTVA
                     AGRIMRDLNISSRRKGVKDEIAAVLILQSYLDSIIIKEEKNNGE"
     gene            7481..7780
                     /locus_tag="PLI11_00895"
     CDS             7481..7780
                     /locus_tag="PLI11_00895"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPZ51453.1"
                     /translation="MANEIEAGQIFELTDEEGNTFTFELLDFVAVEEELYAVITTVEE
                     EEKDDDEMNVVIMRVEIENKEPIFTLVEDEEVCKNVLEAFISQFEDEEEEEVESE"
     gene            7801..8697
                     /locus_tag="PLI11_00900"
     CDS             7801..8697
                     /locus_tag="PLI11_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPZ51454.1"
                     /translation="MYHYFIINPVAGRIQHEEVIEILKDVLKTESYAYNVTEHVWHAR
                     QLAFEAAANHADIRIYSIGGDGTLNEVANGIVESGNKEAVLVPIPLGSGNDFVRSLGY
                     KKEKDLRKLIDRAVSCSYIEIDLCKANDRYFLNISSLGFDAQVVANAIAYKKNKFIPK
                     GLAYYLSVFTTFVRMKFYPLTITVDDKETFDNNTTLLAVANGRYYGGGIIPVPGADIQ
                     DGCFDVCLVDKVRRIKVPIFFPMYAKGNHGKLKEVNFFKCRKLHVKCNNGFVSLNIDG
                     ELSKVKEVLFEVVNRKIRVGQI"
     gene            8698..9627
                     /locus_tag="PLI11_00905"
     CDS             8698..9627
                     /locus_tag="PLI11_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HPZ51455.1"
                     /translation="MTRVLTVTLNPSIDKTVYIKGFKLGATNYIENTHIDMGGKGINV
                     SKALFNFGIDNIATGFMTESHVDKIDRIGIRNRFYLVEGALRVNTKINDLSTGTVTEL
                     NEKGFCIHKKDLTAFIELYEELCPKKGIVVLSGSIPKGVPDDIYAILIKIAKKKGSKT
                     LLDAHGRFLVYGLKENPYAIKPNVFELESIVDRKLKDINDIVDAVKEIKDGATLIAVS
                     MGKEGALFFKEGRFLFAETFPIQSISTVGAGDAMTAAMVLSIINGFDLDTTARFATCA
                     GTMTCLKEGSNVCSHEDVLDNINRVSLKYLQEG"
BASE COUNT         2742 a         1816 c         2169 g         2947 t
ORIGIN      
        1 cttaagttgc ttgacacgtg gattttcttc aagaggcatc aatacttccg gctctctgtc
       61 ttccatcttc tcgacatgct cgttggagaa cataaggtct tctatagcgc gtgggttctt
      121 tacaagatcc atgcgtggtg ataccttttc gtccacagac atagcagtga gttttgtaat
      181 caatgtagta acctgttcgc tgatttcgtt gaactggtct tctgtagcaa cctttgcatc
      241 gatcagcttg ttcttgtact cgtcgatagc gtttgcctcg atccaagcgt cgatttcgtc
      301 cttgcttctg tagctggatg catcggaagg tgagtgtccg gatactctgt aggtgagtgt
      361 atctagaagc actggacctc tcttttcttc gattattctc ttctttcttc tgatagcgtc
      421 gattactgcc agtgggttgt atccgtcaac acgctctgcg tgcatcatgt ctggatttac
      481 gccagcgcca aatcttgcaa gtaatccttg tcccatggtt tctccgctgg tctgtcctcc
      541 cataccgtac tggttgttga agaagttgaa tatgatgggc agtccgccgt tcatatcgcc
      601 ttcccaaagc tgggtgaatt ggtccattgt tgcaaagcac attgcttccc aaaccgggcc
      661 tcttccaagg gatgcgtcgc ctatgttcgc aataacaatg ccgttctttc tgtttacctt
      721 cttgaacagg gcacatcctg ttgcaatagt tgcacttcca ccaactattg cgttgtttgg
      781 atagattccg aagggtgtga agaatgcgtg cattgaaccg ccaagacctc tgtggaaacc
      841 ggtatcgcgg gcaaaaattt ctgcgagtgt tccatagata aggaagtctg tagcaagttc
      901 ctttacactc ttctggtctt tctcaactat tttaagtatt gcaccatcaa agtaactttc
      961 catgatttcc atgagcttgt tgtcgtccag ttgatgaatg gctgaaagac cttttgcaag
     1021 aatttcacca tggcttctgt gggaaccgaa gatgaaatcg tcaactgtca aaaggtaagc
     1081 ctgaccaaca gcggaagctt cctgaccaat tgacagatgg gcaggtcctg gatggttgta
     1141 ggcaataccg ttgtattcgc cggtggtttt aatgctgtta atcataccct caaattcatt
     1201 aataattttc atatgggtat agattctcag aaaatcgtcg gtagtatagt tttctttttc
     1261 ttcctcgatt gatttattgt attggtttac aggtatctta ccaaactcaa tccaatcgct
     1321 ttttctaact tcttttggat caataaatag agatttaggc atttcataca ctcctttata
     1381 ctttaaaatt caaatattcc ttctctaatg acttcacaaa ctgttggatg agggaatacg
     1441 atttccttga tgtcgtcaat tctcatctgg gtttcaatca ttattccagc accgtaaatt
     1501 atttctgatg aataattgcc aatcatgtga acgccaataa catttctata cttcttgtca
     1561 atgagtatct tgatgatgcc gtcgccacct tcgttttcag caacgtatct gcctgaatac
     1621 atcattgaaa gcttgacaac ttcaaagtca attcccttgg cttttgcagt ttcttcagtt
     1681 tcgccaaccg cagcgacctc ggggttggtg tagataacgg atgggattgc gttgtagcgc
     1741 atgatatcct tcttgccaag aatgtcattc aaggcaactt cggtttccct gtaagcggtg
     1801 tgtgccagca tggatttgcc gttgacgtcg ccggctgcgt atacgccagc aatatttgtc
     1861 ctgctgcgct catcggttat gattctacct ctttcaacct ctacgcctat ggtttcaaga
     1921 ccaagacctt cggtgaaggc tcttcttccg atgctgagaa gtaccttgtc gcatggaact
     1981 tcgtaggatt tgccatcctt ttcatatatt accatgcctg ccttgacttc agttaccctg
     2041 ctgcccagat tgaaggtgat acctttcttc tggtagttct taagaagaat ttcggatatt
     2101 tccctgtcca tcggacctgc aatcttgttc atcatttcaa ccacggttac attggaacct
     2161 gctgagttga aatatgaggc catttcaagt cctatgactc cgccgccaac aatacacaat
     2221 tcctttggca cttccgtaag atccagaatc tctctgtttg tgaggacaaa tcctgattca
     2281 agaccttcct tgacacctgg aatagggggt acgacaggca cggaacctgt agcaatgagg
     2341 agtttcttgc cggtgtagac atcatcgcca gcctttacgg taaatccgtc agcatttctt
     2401 ccggtgatag taccttcctt catgacaacg gttaccttgt tcttcttaag tgaagaatta
     2461 ataccgccaa caagagtcct tacaaccttg ttctttcttt tgattacata atcatggtcg
     2521 agcttgagtt cctttgcagt aactccgtat ttttctccat ttgcaactcc gtcatagagt
     2581 tttgcagagt agagaagggc ctttgaagga atacatcctt cgttcagaca aactccgccg
     2641 atgaatcttt tttcaacaag cagaacctta aagccttcct tgcctgcgcg ttccgctgcc
     2701 agataaccgg caggtccgcc acctataact atcaaatcat aaaccatata tcacacctca
     2761 tttattttgc tagtaatatt gaaaagtttt caaggttcct gcaaagctcc tgcaagaatc
     2821 ttgcggcagg tgctccgtca agcgccctgt ggtcaaaggt cagtgacaag gtcatggcct
     2881 gatacatctt catttcgcca ttaacattct tgacccttgt ctcgagggtg ttgactccca
     2941 atatgcaagt ctgtggtggg ttgattacag gtgtaaagga ctcgatgccc aaagtaccaa
     3001 gattggttat tgtaattgta ccaccttgaa gcagatcggg attaatattt ccgctttgcg
     3061 ccatctgtgc aattgatttt gcctcttttg aaatctcgtt aagagacttt ctctctgcat
     3121 tgaacagggt tggaaccata agtcctctag gtgtatccac tgcaacaccg agatttacag
     3181 tgttgaagta tctcattgtg tcatcaagga aatgagcatt gcaatccctg tggttgagta
     3241 ctgttctgga cactgcataa agaataatgt cgttgattgt aatgttttcc agaccaagtt
     3301 tgtccttgtt ggccttgacc tgttttctga actccataat gtttgtagca tcgaatgaag
     3361 cattcagtgt aagttgggcc atgctggaca aggagtgatg cattgctttt gcaattacct
     3421 tcctaatatt aggcagcttt acatcttcgt actctacaaa tggagcagct tcctttacag
     3481 gtgcaggtgt gctggcagct acctgtgctt gtggtgccgg ttgtgcagtc aaatcggaag
     3541 tggtaactct tccaccaatg cctgttccgg ccatatcctt tgttgccttg tccattgcag
     3601 cgtatgtggt tgctccacct tgtgcaataa aatcttttat atcgctctct ataattctgc
     3661 cgtaaggtcc cttgccgctg acgaacattg gatttactcc tgctttctct gccagattgc
     3721 gagcgcgtgg ggaaatcatg attctgccgg tatctgtaac acttgctttc tcttccgcag
     3781 caacctgctt tacaggtgct tgtggtgcag ctgcaggtgc tggtgcggcc acttcttctt
     3841 ccacttctgc tacgccgcct ggtatgaaat cggagtagtc gtctccttct tcaccaattg
     3901 cagcaacatt cattaaaact ggtacgtcgt cgccttcttc aaagaagatg gcaagtaaag
     3961 ttccttcgac ttttgcttct tcgtcaaatg tagccttgtc ggtttcataa gtgaaaagta
     4021 tgtcgccaac attaaccttg tcgccaactt ttttgtgcca ctgtgaaata atacagcttt
     4081 caacagtgtt accctgtctt ggcatgataa caggtgttgc attggactgg cctgttgctt
     4141 tttttgcaac tttctcgacc tttacctcca ctttttcttc cgctggcttt atttcttccg
     4201 ctttctgcgt gtcggctcct tcaggtatga attcgttata atcttcgcct tcattgccaa
     4261 taacgcagac atttgttaga accggcacat cgtctccctc ctggaagaag attgcgagca
     4321 aagttccttc gacttttgct tcctcgtcaa aggtagcctt gtcggtttcg taagtgaata
     4381 gaatgtcacc aacattaacc ttgtcgccaa cttttttgtg ccagtttgag attatacaac
     4441 tctcaactgt ctgcccttgt ctaggcatta ttactgctgt agccatagtt gacctccttg
     4501 caaagtttaa aattatcacg gaatgttact ttggaacatt ctgcttgtta aattgtaaca
     4561 tgatagtgtt acagtgtcaa tgaaaagatg ttatcatttg atgaagtttt cctttccaaa
     4621 tcatattatt tgttcatgag aaaaacttta tcctccattg atagaaatgg tataccttta
     4681 cagaagtttt tgattaaatt atacttcaat aaaggagttg tgaagcttat gaaattctta
     4741 aacataagac ccaaattgag acttgtattt ttagcatctt atcttgcaac agccgtatgg
     4801 ataatggtaa agaagttttc ctttatatat attgttgcag gactcttgtt tctttttggc
     4861 tgctatattt ctcttgtgaa ggcggaagtc atcaaggatt cgagagcgga caacatagat
     4921 aatttcagct ttgatttcgt tagttttatc ataatattgg tattggtttt tgatattgtt
     4981 tttagcgttt tataggggca taatgttgtt gacaaaagtg gcagattaaa ttactataag
     5041 actatggttt tattttacca atagtaaaag atgaaaagga agtgtgtttt aatgaagact
     5101 tacatgatca tgttgaaatc gatcgacgat gttaaaagat ttgttaatgc agctacaaca
     5161 ctaacatgtg aagcggatct ttcttcaggt cgctatattg tagacgcaaa gtcaatcatg
     5221 ggcatattct cccttgattt gtcaagacca atcaagctgg aactgcactg caacgaggac
     5281 cactgctgcg atggatttgt tgacaaaatc aaggatttca ttgttgagga gtaatctttc
     5341 ttgcaatgaa atatgcactg taaaatgcgc atggtggata atcatgtgct ttttattaca
     5401 gaaggagtat taatgaaggt agcaacatat acgataggat gcaaagtaaa tcaatacgaa
     5461 acagaagcgg ttttggagat gtttcttaat aaagggtata cggtggtacc ctttagcgaa
     5521 aaagcagatg tatatataat caatacttgc agcgtgactc agatgagtga cgcaaagaac
     5581 agacaaatga taagaaaatg ccacagactg aatccaaatg cggtggttgc cgttatggga
     5641 tgctacagtc aggttgcgcc ggatgaggtt gcacaaattg agggcgtgga tattgtaata
     5701 gggacgcaaa acagatcaaa gcttcttgaa atggcggagg aactcctcaa gaacaagaaa
     5761 gaggcaaatg agcctacggt ggatgtaaat gctacggatt tcagggttga actgccgttt
     5821 gaggagctgt caatttcaag atttgaaggc catacaagag cgtttataaa gattcaggac
     5881 ggctgttccc agttttgctc gtattgcata attccatacg caagaggtcc tgtaagaagc
     5941 aggacattgg aaagtctgag aaatgaggtt gagaaggttg ccaacatggg atttttggaa
     6001 gttgtgcttg taggcatcca tttggcttcc tatggaaagg atcttgaacc cagagttaca
     6061 cttaaggacg cagtaagcgc tgcttcaagt gttgaaaaga taaagagggt gaggcttggt
     6121 tctcttgatc ccatgtattt gagtgatgac gttataaaag ctttgtccga aaaccaaaag
     6181 ttttgcagac attttcattt gtcgttgcaa agcggaagtg attccgtttt gaagaggatg
     6241 aacagaaagt atacaacgga tgaatacatg gaaattgtgg acacgattag aaaatacatg
     6301 ccagactgtg ccataactac cgatgttatg gttggttttc ctggcgaaac ggaagaagag
     6361 tttttggaaa gtcttgagtt tgtaaggagt gttggatttt cacgcataca tgtgtttccg
     6421 ttctctccaa gaaaaggtac tgttgcgtat aaaatgaagg atcagattga aaattctgtg
     6481 aagaaggaaa gagtcgacag aatgattgct cttggaatgg acttggagca aaggtttctt
     6541 ggcagcatga caggtaaggt tctggatgta ctgtgtgaga aggattgtgt ggggtatacc
     6601 ggcaattatg taaaggttgt agtcaatgaa gaggatttta aggaaggcga attaattctt
     6661 gtcaggataa ctggtgttac aggtaatgag tgtataggtg aaagaatata aatatactgt
     6721 attgctatga agatgcaagt gtgataatat tcacttagct tagccaatta aggatgggtg
     6781 gttaaaatgg ataacaagac tgtgttttac aaacgggaaa aagaacagca aaacgagaca
     6841 agggtagtgc ttacggaagt atatgaagcc ttgaaggaaa aaggttacaa tcccataagc
     6901 cagttggtag gttatataat gtctggcgac ccaacatata taacgaatca caagaatgca
     6961 agagctctgg taacgagggt tgaaagggat gaaatagtgg aagagctctt caaatattac
     7021 cttgaaacac ctcaagactg atgatgctct atgagagtgc ttggtataga ttacggcgac
     7081 agaagaatag gtgtcagcgt aagcgatgcg tttgggtgga ctgcttcttc attgggattt
     7141 attgatgtca agaaatacaa aaacgaggtt tttgacatta ttggccagta tatcaaggag
     7201 taccagacag aaactatagt tgtggggtat cctttgaaca tgaacggaac aaaaggaccc
     7261 agggcggaaa taacagattt attcataaca caactgacag aaaagttccc ggatataaaa
     7321 accgtgaagt gggacgaaag acttacaact gttgccgcag gaagaattat gagagactta
     7381 aatattagta gtcgaagaaa aggtgtaaaa gacgagatag cagccgtatt gatactgcag
     7441 tcttatcttg acagcataat aattaaggag gaaaaaaata atggcgaatg aaattgaagc
     7501 tggtcaaatc tttgagttga ctgatgagga aggtaatacg tttacgtttg aattgttgga
     7561 ttttgttgca gttgaagaag agctgtacgc agtaattaca acagtcgaag aggaagagaa
     7621 agacgatgac gagatgaatg ttgttatcat gcgcgtggaa attgaaaaca aggaaccgat
     7681 ttttactctc gtagaggacg aagaggtttg caaaaatgtt ctcgaagcat ttatttcgca
     7741 gtttgaagat gaagaggaag aggaagtaga aagcgaataa taataattga gggttaatag
     7801 atgtaccatt atttcataat aaatcctgta gctgggcgca tacaacatga agaagtaatt
     7861 gagatattga aagatgttct taaaaccgaa tcctatgcat acaacgttac cgagcatgtc
     7921 tggcatgctc ggcaacttgc ctttgaagcg gctgccaatc atgccgatat aagaatatac
     7981 tccattggag gggacggcac gcttaatgaa gttgcgaatg gcatcgtgga aagcggcaac
     8041 aaggaagctg ttttggtgcc tataccactt ggctctggaa acgactttgt aagaagtctt
     8101 ggatacaaaa aagaaaagga cttaagaaag cttatagaca gggcggtaag ctgttcatat
     8161 attgaaatag atctttgcaa ggcaaacgac aggtactttt taaatatttc gtccttggga
     8221 tttgatgcac aggttgttgc aaatgcaatt gcctacaaaa agaataagtt tattcccaaa
     8281 ggactggcat actatttaag cgtgttcaca acttttgtca gaatgaagtt ctatcccttg
     8341 actataacgg ttgacgataa ggaaaccttt gacaacaata caactctgct ggctgtggca
     8401 aatggcagat attacggtgg aggtataatt ccagtacctg gagccgacat tcaagatgga
     8461 tgtttcgacg tgtgtctggt ggacaaggta agacgaatca aagtccctat tttctttccg
     8521 atgtacgcaa aaggtaatca tggcaaattg aaggaagtga atttcttcaa atgcaggaag
     8581 cttcatgtaa aatgtaacaa tggatttgta agtctgaata tagatggcga actttctaaa
     8641 gtaaaggaag tattgtttga ggttgtgaac cggaaaataa gggtggggca gatatagttg
     8701 acaagggttt tgactgttac tttgaatcct tccatagaca aaaccgttta tataaaggga
     8761 ttcaaattgg gagcaacaaa ctacatagaa aatacacaca ttgatatggg tggaaaagga
     8821 atcaatgttt ccaaagcctt gttcaatttt ggaattgaca atattgccac aggctttatg
     8881 acggaaagcc atgttgataa aatagacaga atcggcatac gtaacagatt ttatctggtg
     8941 gagggagcct taagggtaaa tacaaagata aatgatttga gcacaggtac tgtaacagag
     9001 cttaatgaga agggtttttg catacacaag aaagatttga ctgcatttat tgaattatat
     9061 gaagaactat gccccaaaaa gggcattgtt gttttatcag gaagtattcc aaagggtgtt
     9121 cctgatgata tttatgcaat tcttattaag attgcaaaaa agaagggttc aaaaaccctg
     9181 ctggatgctc atggcaggtt tcttgtatat ggtttgaagg aaaatccgta tgcaataaaa
     9241 ccgaatgtat ttgaacttga atccattgtt gatagaaagt tgaaagatat taacgatata
     9301 gttgatgcgg tgaaagaaat aaaagacggt gcaactttga tagctgtctc catgggaaaa
     9361 gaaggagcac tgtttttcaa agagggaaga ttcctatttg ccgagacttt tcccatacaa
     9421 agcataagca ctgttggtgc aggtgatgcc atgacagctg caatggtgtt atcaataatt
     9481 aatggttttg atttggatac aactgcaaga tttgcaactt gcgctggcac aatgacctgt
     9541 ttaaaagaag gaagtaatgt gtgcagccat gaggatgttc ttgataatat caacagagtt
     9601 agtttgaaat atctacagga gggataacca tgtccttttt tattgatgca aagccaatct
     9661 ttttgaagga tctt
//
LOCUS       DAONVY010000016         9385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_5610_length_9385_cov_4.219507, whole genome shotgun sequence.
ACCESSION   DAONVY010000016 DAONVY010000000
VERSION     DAONVY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_9385_cov_4.219507"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..494)
                     /locus_tag="PLI11_00910"
     CDS             complement(72..494)
                     /locus_tag="PLI11_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51456.1"
                     /translation="MSKNYDDIINLSRPPSKRPKMSRQERAFQFSPFAALTGHDSAIM
                     ETARLTDKKIELDDYMKEVINQKIIMIAEWINDHPELIITYFKPDEKKTGGKYVSIRG
                     YVRKIDEYKRTIIMSDNTIISLEDILDIEGQLFNMFCD"
     gene            complement(491..2011)
                     /locus_tag="PLI11_00915"
     CDS             complement(491..2011)
                     /locus_tag="PLI11_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HPZ51457.1"
                     /translation="MENRTYIAIDLKSFYASVECMERGLDPLSTNLVVADESRTEKTI
                     CLAVTPTLKAYGIPGRPRLFEVVQKVSEINKQRLSKAPGGRFAGSSFDDKELKLSPHL
                     SLDYIIAPPRMAHYIDYSTRIYNIYLKHVAAEDIHVYSIDEVFIDATHYLDTYKLTGR
                     EFATRILLDVLGNTGITAAAGIGTNLYLAKIAMDIQAKRTPVDEHGVRIAELDEKSYK
                     KELWSHRPLTDFWRIGKGYAKKLEQHGMYTMGDIARCSLGKPSDYYNEDLLYKLFGVN
                     AELLIDHAWGYEPCTIEDIKAYKPETNSLCSGQVLTEPYTFDKARLVAREMTDLLVLD
                     LVDKRLLTSQIVMTVGYDIENLTNAEIRKTYKGPITTDSYGRTVPKSARGTINLPKQT
                     SSTKMITEAATELFDRIVDKNLLVRRITITFNRVVDEKTNQEEKVCEQLDLLAFYNSL
                     QDGINVEKQEEKEKKIQQAILGIKKKYGKNAVVKGMNLEEGATAMDRNKQIGGHKA"
     gene            complement(2040..2378)
                     /locus_tag="PLI11_00920"
     CDS             complement(2040..2378)
                     /locus_tag="PLI11_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51458.1"
                     /translation="MKKIMFYGMTGEKMCFQHILLNALDLAGSSFEVKIILEGASVKL
                     VSVFEFEKNPLYMRAKEQGLIAGVCLACSKVLGVYEKNLESGLNLLDDMNGHAGMKRY
                     IQDGYYVISI"
     gene            2514..2590
                     /locus_tag="PLI11_00925"
     tRNA            2514..2590
                     /locus_tag="PLI11_00925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2548..2550,aa:Pro,seq:cgg)
     gene            2664..2741
                     /locus_tag="PLI11_00930"
     tRNA            2664..2741
                     /locus_tag="PLI11_00930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2699..2701,aa:Pro,seq:ggg)
     gene            2864..3664
                     /gene="proC"
                     /locus_tag="PLI11_00935"
     CDS             2864..3664
                     /gene="proC"
                     /locus_tag="PLI11_00935"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPZ51459.1"
                     /translation="MRTIAVLGCGNMGGAIIRGIASGREKIANIKVYDKDLSKVEAIC
                     DETGCISCSSEIDAVNGYEVVFIAVKPDMVRKLLGEIKDACKGKIIVSIAAGVNVYTF
                     LDILGHNAKVVRTIPNLPAVVGEGLAGLFFHNVSDEDKVYVERLFSSFGKTVTVYKEE
                     QIDEMIAVTSSSPAYICMFVEALADGAVRAGFNRDDAYKMALQTIYGTAKLMLEKNIH
                     PAVLKDQVCSPGGTTIEGVAVLEKAGFRNAVLEAMTAVSDKVKVLSKG"
     gene            3672..4112
                     /gene="rnhA"
                     /locus_tag="PLI11_00940"
                     /pseudo
     CDS             3672..4112
                     /gene="rnhA"
                     /locus_tag="PLI11_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
     gene            4230..4682
                     /locus_tag="PLI11_00945"
     CDS             4230..4682
                     /locus_tag="PLI11_00945"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPZ51460.1"
                     /translation="MPDDTVARREIVSHPGGAAICAVDDEGYVYTVVQYRIATSGELI
                     EIPAGKVEKGEDPKECAKRELAEETGLIAKEVRHIVSFYATPGYCTEMLHIYLATGLE
                     EGNLNRDEGEFLHVRKYKIDELMRMIQNGEITDGKTIIAAMWAKMNWR"
     gene            4694..5950
                     /locus_tag="PLI11_00950"
     CDS             4694..5950
                     /locus_tag="PLI11_00950"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPZ51461.1"
                     /translation="MYDIIHKKRHGKKLSEDEIKYFVTEYTKGNIPDYQASALLMAIA
                     INSMDKEETSYLTKWMVNSGEVLSLDSIPGKKTDKHSTGGVGDKTTLICAMIVAALDV
                     PVAKMSGRGLGHTGGTIDKLESIPGFRTSLTRQEFIELVKKNNIAICGQSESLAPADK
                     LLYALRDQTATVDSIPLIASSIMSKKIAAGSDCIVLDVKVGKGAFMKTFDEARVLADL
                     MCEIGASMNRKAVSVISDMNEPLGYAIGNSLEVVESIETLKGNGPEDLCSIAIKLAAN
                     MVSLAKDIGYEDAERLCKEVLENGKALGKFREFVKCQDGDTRVVDDYTVFKQPRRVFE
                     YNSEKSGMIKSIDSYKIGNAACILGAGRTVKDGKIDPSAGIVLERKSGERVEKGECLA
                     KLYTDIDNIDNAINILNTAWEIEEGV"
     gene            5955..7169
                     /locus_tag="PLI11_00955"
     CDS             5955..7169
                     /locus_tag="PLI11_00955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPZ51462.1"
                     /translation="MKTKRVFSLLLCMILVFTSYVGIFATGYSQTEANSIYSNADVKD
                     LSAKGFSLLDFDSGTIVYDGNGDKKFPVGNMIKLMTLYLAFEAISNGQTTLDTEFAVV
                     QSAQDKSIGRERVFLDAGKKERITVGQAIEAICIASANDAAYALAHYIGKNEDGFVEM
                     MNKKALELGLKNTFFTDSAGIEDGHYMSANDAAILSYHLIKNYPDVLNYTIKTYGEFE
                     HWSTGQEKTMMVSSNHLIKFYNGSDGLITGYSKEDGYAGVGTISVDEKRVIAVVIGVE
                     TQSHRAAELKKLLEYGLSEFEYRVIDEAGTFVRRVPIKDGVKKQIATATASDFAIVLQ
                     KSKFDKIAREVTVSRNLKAPIKKGEVVGEVVYKLDDEEIGRVDIVADEDMGRVGWFKR
                     LIRKILAWLGLD"
     gene            7241..7426
                     /locus_tag="PLI11_00960"
     CDS             7241..7426
                     /locus_tag="PLI11_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPZ51463.1"
                     /translation="MANNKNKSAFNQMKFEVAKELGIDLKEGYNGDKTARENGAIGGR
                     ITQKVFEQYTGKNYKNQ"
     gene            complement(7475..8125)
                     /locus_tag="PLI11_00965"
     CDS             complement(7475..8125)
                     /locus_tag="PLI11_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPZ51464.1"
                     /translation="MSYLIGEFRNMLLSIPGIIFIFSFKGFFQSFVAGKLGDPTPRNA
                     GRLTMNPAAHIDLIGFIFIILFGIGWAKPVPVRTRFFKKIKRDVAIFYISGPISCIIG
                     GFILFFLSALSESLLPISMVSDIISLMLYLGCTISLSLGMFYLLPLPGLDGYYFITNF
                     VPYKYNNVLYNIEKYSMFIFIGFILLLRSTPLGYYLIHTPVSLMINFFALILSPIF"
     gene            8260..9051
                     /locus_tag="PLI11_00970"
     CDS             8260..9051
                     /locus_tag="PLI11_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPZ51465.1"
                     /translation="MRLFLEPVKIRLDNFEGPYDLLLSLLEKNKLEITEISITEIADQ
                     YIEYISSAGGFDLDIASEFLVMGATLIHMKSKRLLPKPEEEEEELTAEELARRLSLYK
                     SIKQASYTLEKDMEFWSQCLYKKPEELNFPRREEILDLNIFELSNCMTILQQRLKDRR
                     QDTSKKMEQILEREKVSLKDMMVRVVNVITKKTRTRFSELFSLKRHSKTEVVTGFLAI
                     LELDKGKKIKLQQEKLFDEIDIYLNNNGNDNNLEEFLNSYDDIDM"
     gene            9065..>9385
                     /locus_tag="PLI11_00975"
     CDS             9065..>9385
                     /locus_tag="PLI11_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPZ51466.1"
                     /translation="MKNYEAIIEALLFASGHKVEASEMASVLGLDNKELIDIMDRLIK
                     KYDENNGGLMIRKIKDGYQMCSRPEYHDDIKEYFEQPQKQGLTRAAMETLSVVAYNQP
                     ITRAG"
BASE COUNT         2971 a         1538 c         2104 g         2772 t
ORIGIN      
        1 ctaagagatc gcaacaaaac atgtattgcg atctcttaag ttaatgacat tggcaaatag
       61 ccgcttttat atcaatcaca aaacatatta aacaattgac cttcaatatc tagtatgtct
      121 tctaaagaaa ttattgtatt atctgacatt atgattgttc ttttgtactc gtcaattttc
      181 cttacatagc ctcttatgct tacatattta ccacctgttt tcttttcatc aggtttgaaa
      241 tacgttatta tcaattcagg atgatcgttt atccactctg caatcattat gattttttga
      301 tttattactt ccttcatgta gtcgtcaagt tctatttttt tgtctgtaag tcttgcggtt
      361 tccattatag cagagtcgtg acctgtaaga gctgcaaagg gtgagaactg aaaggctctt
      421 tcctgcctgg acatttttgg tctttttgat ggaggacgag atagatttat tatgtcatcg
      481 taattcttac tcatgcctta tgccctccta tttgcttgtt tctgtccata gcagttgctc
      541 cttcttccaa gttcattcct tttacaacag cgttttttcc gtacttcttt tttattccca
      601 atatggcttg ttgtatcttt ttttcttttt cctcttgctt ctccacattt attccatctt
      661 gcaaagagtt ataaaatgct agaagatcca gctgctcaca aaccttttct tcctggtttg
      721 tcttttcgtc aacaacacga ttgaatgtta ttgtaattct ccttacaagc aagtttttgt
      781 ctacaattct atcaaaaagt tcagttgccg cttctgtaat cattttggtg gaggaggttt
      841 gttttggcag gtttattgtg cctcttgcgg actttggaac ggttctgcca tagctgtcag
      901 tggtaattgg ccctttatat gtttttctta tctctgcatt tgtcaggttt tcgatatcat
      961 atcctactgt catgacaatc tggctggtta atagtctttt gtccaccaaa tcaagaacca
     1021 gaaggtcagt catttctctt gcaacgagtc ttgccttatc aaaagtatac ggctctgtca
     1081 atacctgacc ggaacacaga ctgtttgttt cgggtttgta cgctttgata tcttcaattg
     1141 tacatggctc atagccccag gcatgatcaa ttaatagttc agcattaact ccaaacagtt
     1201 tgtaaagcaa atcttcgtta taatagtctg aaggtttacc taatgagcat cttgcgatat
     1261 ctcccatggt atacatgccg tgctgttcca gtttttttgc atatccttta cctatgcgcc
     1321 agaaatctgt caatggtctg tgggaccaca actccttttt gtagcttttt tcatcaagct
     1381 ctgcaatcct gacaccgtgt tcatccaccg gggtgcgctt tgcctgtata tccattgcga
     1441 tttttgcaag atatagattg gtacctattc cagcagctgc ggtaatgccc gtatttccaa
     1501 gtacatcaag gagaattcta gttgcgaatt cacgacctgt gagtttatag gtgtccaagt
     1561 aatgtgttgc atctataaag acttcgtcaa tggaatacac atgaatgtct tcggctgcaa
     1621 catgctttag ataaatattg tatattctag tgctgtaatc gatataatgg gccattctgg
     1681 gaggtgcaat aatataatcc agggaaaggt gaggagatag ttttaattcc ttgtcgtcaa
     1741 aggaggaccc tgcaaatcta ccacctggtg ctttggagag acgctgtttg ttgatctcac
     1801 ttactttttg cacaacctca aaaagcctgg gtctgcccgg aattccatag gctttgagag
     1861 ttggtgtaac tgcaagacat atggttttct cggtgcggct ttcatccgct acaaccaggt
     1921 tagtggaaag aggatccagt ccgcgttcca tacattctac agaagcgtaa aaggacttca
     1981 aatctatcgc aatatatgtt ctgttttcca aagttgttat gtctcctcct tcaaaataat
     2041 tatatactta tcacatagta tccatcctgg atgtacctct tcattcctgc atgcccgttc
     2101 atatcgtcta gtagattaag tcctgattca agatttttct cgtaaacgcc caataccttt
     2161 gagcatgcaa ggcaaacgcc tgcaattaat ccttgttctt tcgcccgcat gtatagtggg
     2221 tttttctcga attcaaatac tgaaacaagt tttacagatg caccttcaag tatgatcttt
     2281 acttcaaaag aacttccagc caaatccaat gcattaagca ggatatgctg aaaacacatc
     2341 ttttcgcctg tcattccata gaacattatt ttcttcattt catgctcctc cttttatgta
     2401 tatatgtaga ttatatcata aaagcagggt atacgatatt agaatcacat atgacctgca
     2461 atttaaatgg tctttacatt cgttttcttt tgtgctagaa tttataattg tttcggggtg
     2521 tagctcagtt tggtggagcg cttgcttcgg gagcaagagg ccgcaagttc gaatcttgtc
     2581 actccgacca ttaaatgtaa ttcccttcca attttacagg gctttacaac caatttcatt
     2641 tatgctataa tacatgtcgg cgtcggggtg tagctcagct tggctagagt gcttgcttgg
     2701 ggtgcaagag gtcgcaagtt caaatcttgt cactccgacc ataaagactg tcaatacaaa
     2761 cgacagtctt tttttatatg ctttgaaaat ggtataaatt aactgttgtt attgaaaata
     2821 tgttgtttta agtgtataat aaataaaaaa aggtgttgga tttatgagaa caatagcagt
     2881 cctaggttgt ggaaatatgg gaggtgcaat tataagagga attgcatcag ggcgtgagaa
     2941 gattgccaat ataaaagttt atgacaaaga cctttcaaag gtcgaggcca tctgtgatga
     3001 aacaggatgt atttcatgca gcagcgaaat tgatgccgtt aatggatacg aagttgtttt
     3061 tattgctgta aaacctgata tggtgcggaa gcttttaggt gaaataaagg atgcatgcaa
     3121 gggcaagata attgtctcaa ttgctgcagg agtaaacgtg tatacttttc ttgatatatt
     3181 gggtcataat gcaaaagttg tccgtacaat tcccaatctg ccggcagtcg ttggagaagg
     3241 acttgcggga ttgttctttc ataatgtaag tgatgaagat aaggtatatg tagagagact
     3301 gttttctagt tttggaaaga ctgttactgt atacaaagaa gagcagattg atgaaatgat
     3361 tgctgtcaca tcaagctcgc cggcatatat ctgcatgttt gttgaagctc ttgcagacgg
     3421 agcagtgcgt gcaggattca atagagatga tgcttacaaa atggctttac aaaccatata
     3481 cggaacagca aaacttatgt tggaaaaaaa cattcatcct gcggtattga aggatcaggt
     3541 atgttcgcct ggcgggacca ctatagaagg agttgccgta ttggaaaaag caggttttag
     3601 aaatgcggtt ctggaagcca tgacggcagt gagtgataaa gtaaaggtat tgagtaaagg
     3661 ataaggtgga aatgaaaaaa gttcagattt atactgacgg cgcatgttcg ggaaacccag
     3721 gtcctggagg gtatgctgcc atactcatat acgacaagca tgaaaaagag atatggggat
     3781 atgaagacaa tacaactaac aacagaatgg agcttatggc accaataaaa gccttgtcga
     3841 tgttgaaaac caaatgtgaa gttgaaattt attcggatag tgcatatgtt gtaaacagtt
     3901 tcaatatggg atggatatat gcctggaaga aaaacggatg gaagaaggac aagggtcagg
     3961 agctaaaaaa tgttgacctg cttcaaaaat tgtatgctct ttgtcaaaag cacaaagtta
     4021 cctggcacaa tgtaaaggga cacagtgaca atgaatacaa caatcgctgc gatgctcttg
     4081 cggtaaatgc aataaaggaa ctgataaaaa aaaagaagga aaacaatgaa aatagtggat
     4141 tttatgaagg agatttgaag gaaactgtca tatcaataga ggaaatattc aaaggcaagg
     4201 ttttcgatgt gaatgtattg caggtaaaaa tgcctgatga cacggtggct cgcagggaga
     4261 ttgtttcaca tccgggagga gcagccatct gtgctgttga tgatgagggt tatgtgtata
     4321 cggttgtcca gtacaggatt gcaacaagcg gtgagctgat agaaatacct gcaggcaagg
     4381 tggagaaggg tgaagatccg aaagaatgcg ccaaaaggga actggctgaa gagacaggtt
     4441 tgattgcaaa agaagtaaga catattgtaa gcttttatgc aacacctgga tactgcacag
     4501 aaatgctcca catatatctt gctacaggtc tagaagaggg taatctcaac agagacgagg
     4561 gcgaatttct tcatgtaaga aaatacaaaa tcgatgaatt aatgagaatg attcaaaatg
     4621 gggaaattac ggatggtaaa acaataattg cagcaatgtg ggcaaaaatg aattggagat
     4681 gattcatttg agaatgtatg acattattca taaaaagaga catgggaaaa aactaagtga
     4741 agatgaaatt aagtattttg tgaccgagta tacaaaaggg aacatacctg attatcaggc
     4801 atctgctctt ttaatggcca ttgcgataaa ctcaatggac aaagaagaaa cttcgtatct
     4861 taccaagtgg atggtgaatt ctggggaggt actgagtctt gattcgattc ccggcaaaaa
     4921 gacagacaaa cacagtacag gaggtgttgg ggacaaaacc acacttatat gtgcaatgat
     4981 tgttgcagcc ttggatgtgc ctgtggcaaa aatgtcagga aggggactgg ggcacacagg
     5041 cggcactatc gataaacttg aatccatccc cggattcaga acaagcctga caagacagga
     5101 atttattgaa cttgtaaaaa agaacaatat tgccatatgt ggtcagtcgg agtcccttgc
     5161 gcctgcggac aaattactat acgcattaag agatcagaca gcgactgttg acagtattcc
     5221 acttattgca tccagtataa tgagcaagaa gattgctgca ggcagcgact gcattgtatt
     5281 ggatgtaaag gttgggaaag gcgcatttat gaaaaccttt gatgaagcaa gggttttggc
     5341 tgaccttatg tgtgagattg gcgcatccat gaatagaaaa gctgttagcg tcatatccga
     5401 tatgaacgaa cctctcggtt atgcaattgg caattctctt gaggttgttg agtcgattga
     5461 aacattgaag ggaaacggac ctgaagatct ttgcagcatt gcaatcaagc tggccgccaa
     5521 tatggtgtct ttggctaaag atattggcta tgaggatgct gagaggttgt gtaaggaagt
     5581 attggaaaac gggaaggctt tgggaaagtt tcgtgagttt gtcaaatgtc aggatggaga
     5641 tacgagagtt gttgacgact atacagtatt caagcagcca agaagagtat ttgaatacaa
     5701 ctctgagaaa agtggcatga ttaagagtat cgattcgtat aagattggaa atgctgcatg
     5761 tatacttgga gcaggcagga ctgtaaaaga tggtaagatt gatccctctg caggcatcgt
     5821 tttggaacga aagtcaggcg aaagagttga aaagggcgaa tgtttggcga aattgtatac
     5881 cgatattgac aatatcgata atgcgataaa tatattgaat actgcctggg aaattgagga
     5941 aggagtgtga ttttatgaaa acaaaaagag tgttttcttt attgctttgc atgattctag
     6001 tttttaccag ttatgttggt atttttgcga caggctattc tcaaactgag gcaaactcaa
     6061 tatattccaa tgctgatgtg aaagacttga gtgcaaaagg tttttcgctt ctggattttg
     6121 attcgggtac cattgtttat gatgggaatg gagataagaa atttcctgtt ggaaacatga
     6181 tcaagttaat gacactctat cttgctttcg aggcaattag caacggtcaa acgacattgg
     6241 atactgaatt tgccgttgta caaagtgctc aagataaatc cataggaaga gaaagagtgt
     6301 ttttggatgc aggtaaaaag gaaagaataa cggttggcca ggcgattgaa gcaatatgca
     6361 tagcgtcagc aaatgatgct gcatatgctc ttgctcatta catagggaaa aacgaagacg
     6421 gtttcgttga aatgatgaac aaaaaggcgc tggagctggg actaaagaat accttcttca
     6481 ccgactctgc aggtattgaa gatggacatt acatgtcagc aaatgatgct gctattctgt
     6541 cgtatcactt gataaagaat tatccagatg ttttgaatta caccataaag acctatggag
     6601 agtttgaaca ttggtcaacg ggtcaggaaa aaacaatgat ggtgtcgtca aatcatctga
     6661 ttaaatttta caatggaagc gatggtttga taacaggtta ttccaaagag gatggatacg
     6721 ctggagttgg aacaatttcg gttgatgaaa agcgagttat tgctgttgta attggtgttg
     6781 aaactcagag tcacagagct gcagaattaa aaaagcttct tgagtatgga ctttccgaat
     6841 ttgagtacag ggttatagat gaagcaggta cttttgtcag aagagtgcca attaaggacg
     6901 gggtaaagaa gcaaatagcg acagctacgg caagtgattt tgcaattgtt cttcaaaaat
     6961 caaagtttga caaaattgca agagaagtca cagtgagcag aaatttgaaa gcacctatta
     7021 aaaaaggtga agttgttggt gaagtggttt acaagctgga tgatgaggaa attggcagag
     7081 tcgacatagt tgcagacgaa gatatgggaa gagttggctg gttcaaaagg cttatcagga
     7141 aaatacttgc atggctcggg cttgattaga aaggtataaa atatatttta gatggcataa
     7201 tattatcgca acacaaaaat taagtaagtg tggtgatatt atggctaaca acaaaaacaa
     7261 atctgcattc aatcagatga agttcgaagt tgctaaagaa cttggtattg atctgaaaga
     7321 aggctacaat ggcgacaaaa cagcacgcga aaacggtgcc attggcggtc gtataactca
     7381 gaaggttttt gagcaatata ctggaaagaa ttacaagaac caataattcc ttagataaaa
     7441 caaggtatcc cgatttttgg gataccttgt ttgactagaa tatgggtgat agaattaagg
     7501 cgaaaaaatt aatcataagt gatacaggtg tgtgtataag gtagtagcca agtggggttg
     7561 aacgcagaag aagtatgaat ccgataaaaa tgaacatact gtatttttct atgttataga
     7621 gtacattgtt gtatttgtat ggcacaaagt tggttatgaa atagtagccg tcaagtcctg
     7681 gaagtggcag taggtaaaac attcccaaag acaaagaaat agtgcaaccc agatataaca
     7741 tcaatgaaat aatatccgat accatgctga taggtagcag ggattctgat aatgcactca
     7801 aaaagaacag aataaaccca cctataatgc aggatatggg tcctgatata tagaaaattg
     7861 ctacatctct tttaatcttc ttgaagaacc tggttctaac tgggacaggt tttgcccatc
     7921 ctataccaaa taaaataatg aaaatgaacc ctatgaggtc aatatgagcc gctggattca
     7981 ttgtaaggcg tcccgcattt cttggagttg gatcaccgag ttttccagca acaaaggatt
     8041 gaaagaatcc tttgaatgaa aatataaaaa tgataccagg gatgcttaac agcatgtttc
     8101 tgaattcgcc tatcaagtaa ctcatgattg tctcctttgt aatatgtgat gtgtttttct
     8161 ttgaaatgat atcatataaa tattggcaaa tcaaatattt ttaatttaca aacatctccg
     8221 ttttatatat aatacctcat gtgaaacgta gaaagaggtg tgagattatt tttggaacct
     8281 gttaaaatac gtttggataa ttttgagggt ccttatgatt tactgttatc cttactggaa
     8341 aaaaacaagc ttgaaataac tgaaatatcg attactgaaa tagctgatca atatattgaa
     8401 tacataagca gcgcaggcgg atttgatctt gatattgcca gcgagtttct tgtaatgggt
     8461 gcaactctta tccatatgaa atcaaagaga ctattaccaa agcctgaaga ggaagaggag
     8521 gagcttactg cagaggagct tgcaaggcgt ctctccttgt acaagagtat taaacaggct
     8581 tcatataccc tggaaaaaga tatggagttc tggtcacagt gtctgtacaa gaaacctgaa
     8641 gaacttaact ttccaagaag ggaagaaata ttggatttga atatatttga gttgtcaaat
     8701 tgcatgacaa ttttacagca acgtttgaag gacagaaggc aggacaccag caagaaaatg
     8761 gagcaaatcc ttgaaaggga gaaggtaagt ctaaaggaca tgatggtaag agtagttaat
     8821 gtaattacca aaaagacaag gactaggttc tccgagctgt tttcattgaa acggcatagt
     8881 aagacggagg ttgttacagg ttttcttgca atattggaac ttgataaggg aaaaaagata
     8941 aaacttcagc aagagaagtt gtttgatgag atagatattt atcttaacaa taatggaaac
     9001 gacaataatt tggaagagtt tctaaattca tatgacgata ttgatatgta aaggatggta
     9061 gtggatgaaa aactacgaag caataattga ggcgttgctt tttgcatcag gccataaggt
     9121 ggaagcgagt gaaatggcgt ccgttttagg gttggataat aaagagttga ttgacataat
     9181 ggacaggctt ataaaaaagt atgatgaaaa caatggcggt ttgatgatta gaaagataaa
     9241 agacggatat caaatgtgtt caagacctga gtatcatgat gatataaagg aatattttga
     9301 acagccacaa aaacaaggcc ttaccagagc cgccatggaa actttgagtg tagtcgcata
     9361 caatcagccc ataaccaggg ctggt
//
LOCUS       DAONVY010000017         9229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_5737_length_9229_cov_3.490735, whole genome shotgun sequence.
ACCESSION   DAONVY010000017 DAONVY010000000
VERSION     DAONVY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5737_length_9229_cov_3.490735"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="PLI11_00980"
     CDS             <1..1715
                     /locus_tag="PLI11_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPZ51467.1"
                     /translation="EKKYHWELVYCELYGGGKYKTNQGINYEFSLGLNGLGACATQYT
                     SEYMDVTVFKDGYKYELHFEKGINIGGLKKEKYDYKHSGTIQKWKPDLDVFTDINIPL
                     EFFQTVLKKQAVVNAGVKFKLYDEESNQHFEYIYPKGIVTYVEEITQGKEFTSIQYYE
                     HQTAGRDRADKPEYKVKMQIAFCFNNHVNLIEYYHNSSYLENGGSPEKAVRNAFVSEI
                     DKYIKSEGKYQKDESKVTFADIQDSLIIVTNSFSTMTSYENQTKKAINNKFIQEAMTE
                     FIKQNLEIYFIENKAEADKIIEQVLVNKRSREMAEKQRINIRKKLSGNIDITNRVKKF
                     VDCRTRDIDKRELYIVEGDSALGSVKLGRDSEFQAIMPIRGKILNCLKADYGRIFKSD
                     VIVDLIKVLGCGVEVKSKFNKDLSTFDLDALRWSKIIICTDADVDGYQIRTLVLAMIY
                     RLMPTLIKEGKVFIAESPLFEINCKNKTYFAYNEREKNEIISKLDGKYTIQRSKGLGE
                     NEPEMMWQTTMNPETRRLIKVVMEDVERTNYYFDMLLGDNLDERKRHIEENGHLFMDH
                     LDLM"
     gene            1728..3923
                     /locus_tag="PLI11_00985"
     CDS             1728..3923
                     /locus_tag="PLI11_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HPZ51468.1"
                     /translation="MADNKLYIEQKISETLESNYMPYAMSVIVSRAIPEIDGFKPSHR
                     KLLYTMYKMGLLNGPRTKSANVVGETMKLNPHGDMAIYETLVRMTKGNEALLHPYVDS
                     KGSFGKVYSRDMAFAAARYTEVKLDKLCTELFGELDKDTVDFVDNYDGTMKEPTLFPV
                     TFPTILVNSNQGLAVGMATNICSFNLVEICEATSALIDNPEENLLKIIKAPDFSTGGI
                     VIYNKKDMNRIIETGKGPIKVRSKYKYDKKNNCIDVYEIPYTTTVEAIIDSVSDLVKT
                     NKIKEVVDIRDETDLKGLKLTIDIKRNTNPDDLMNKLYMMTPLQDTFSCNFNVLIKGK
                     PQLLGVRQLLLEWIEFRIVCMKRKLANEIKTKENRLHLLRGLEKILLDIDKAIKIIRE
                     TETDALVIPNLMWGFGIDEIQANFIAEIKLRNLNKEYILDKVSEIENLINDINDLKDT
                     LGSDKKIKELIKTKLQEVAKKHGKPRRTDLMHEDEVVVVEEEEMIDDYNLKVFLTNDS
                     YFKKIPLVSLRSASDQKLKDEDFIVQEMEWHNKSDIIFVSNKQVVYKCKLHELPDCKA
                     SSLGEYLPNILEMDDDEKIHYMIVTDNYAGFILFAYENGKVSKIPLNSYETKTNRRKL
                     LNAYGSSAPLVDVIFTHEDLDIALVSSNDRALVINSSMIPLKTTRSSQGVQVMRLRKG
                     CVLQSMMKVSETSFKEPKVYRVKNIPAAGPFIKAHDREIQQMSLLNETE"
     gene            complement(3954..5219)
                     /locus_tag="PLI11_00990"
     CDS             complement(3954..5219)
                     /locus_tag="PLI11_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPZ51469.1"
                     /translation="MQELVNNLNHPSRETRLESLRELMKMVEDGKTPKPDMGNDVNNH
                     IHTTYSFSPYSPTKAVWMAYMAGLKTAGIMDHDSLSGAKEFIEAGKIVGMMTTIGVEC
                     RADMSKTRLNGLRINNPDQKSVAYMALHGIPHQNIDTVVEFFKPYTAHRNTRNRKMTE
                     NINKIFAPYEISMDYDKDVLPLSMNHDNGSVTERHLLFALSKKITDKFGKGQKVVDFL
                     KNELNLNLSKKVENYLLQEDNDVYEYDLLGALKSDLVEKFYIDATDECPDVRDVIELG
                     RKVGAISAYAYLGDVGDSVTGDKKTQKFEDDYIDLLFDVIDELDFNAVTYMPSRNTME
                     QLKTVRTYCDKYNLFQISGEDINSPRQSFICEAQRNPEFANLYDATLALINHEKAATE
                     NIENAMFSEKTIKKYPDIKERIQVYKNMN"
     gene            complement(5299..6630)
                     /locus_tag="PLI11_00995"
     CDS             complement(5299..6630)
                     /locus_tag="PLI11_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPZ51470.1"
                     /translation="MKCDVAIIGGGTAGIMASYELNKNIPNLDILIIEQGKPIYERKC
                     PIVQNITRNCISCANCGIMNGFGGAGAFSDGKFNFTTEFGGWLHDYLPREKVMELIEY
                     ADSINVSFGATTERYSTYSDKARELEKKALHNDLHLLMASVKHLGTENNLRILKSMYD
                     YLVEKINIKCNTSVESISVNPDGTFNLQLSDGETVTCKYLIASPGRSGAEWFASQAEK
                     LGIKLINNQVDIGVRVELPAKVFEDITNVVYEAKLVYRTKQYGDNVRTFCMNPYGHVV
                     TENVDGLITVNGHSYSDEKLRSENTNFALLVSNRFTKPFNEPYQYGKRIASLSNMLGG
                     GVLVQRFGDLIKGVRSNEHRMSQSFTRPTLNATPGDLSLALPKRHLDNIIEMLFALDK
                     IAPGTANHDTLLYGVEVKFYSARISLSEMLETPIPNFFACGDGAGVTRGLS"
     assembly_gap    6651..6750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6962..8083
                     /locus_tag="PLI11_01000"
     CDS             6962..8083
                     /locus_tag="PLI11_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPZ51471.1"
                     /translation="MYKKMILVMVAIVMALSVFSGCTGDNDGDGDAVSKLKIGVVYIG
                     NISEPVGFSTEHYKGIMAAAKSLGIKESQILQREEVSDNDESCKEVFESLIANGCNVI
                     IGTSYGYGTYMNDLANDPAYKDIIFMHCSGEYKNDVNFSNFFGRMYQARYLTGIVAGL
                     KTETNKIGYVAAVGIPEVIRGINAFTLGVKSVNPDAEVIVRWTNTWGNPSIEKSTAET
                     LISEGCDVITQHQDTDAAQIAARENKVWAIGYHSDMAVSSKEYNLTSAIWNLEAYYEQ
                     QFTDIINGEWKPGVAYWGGIKEGVVDIAPLSDNVAEGTQAKVDEVLAKFKDGSFDVFA
                     NYEIKDQDGNVKVTADTKLTDEELLSMMWFVEGVVGTID"
     gene            8110..>9229
                     /locus_tag="PLI11_01005"
     CDS             8110..>9229
                     /locus_tag="PLI11_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPZ51472.1"
                     /translation="MQDCILHCFFNFLIICDKGDSVNNVLFELRGITKLFGNVVANKN
                     IDFSVREGEIHALLGENGSGKTTLMNILSGIYTPDSGTIIINGEEVTIKSPEDAIKLG
                     IGMIHQHFKLVDVFSAKENIIMGQSGSIRQNQKNLTREIEELSNRFGLNINLDKKIYQ
                     MSVSEKQTVEIMKVLFRKSKILILDEPTAVLTPQEIDKLFEILRELRDDGCAIVIITH
                     KLNEVMEISDRVTILRKGESVATVETKQTNAKELTELMVGHSVDLSIERPVVEDKTKD
                     VLQVDNLTIFDEDGVKVIDGMSFALDGGEILGVAGVAGSGQKELCEAIAGLHPVAEGS
                     IEFKGRNIVGLNPNEIINLGVSMSFIPEDRLGMGLVANM"
BASE COUNT         2820 a         1699 c         2069 g         2541 t
ORIGIN      
        1 aggaaaagaa ataccactgg gaacttgtct attgcgaact gtacggagga ggaaaataca
       61 aaacaaacca gggcatcaac tacgagttca gtctgggtct taacggtctt ggtgcctgtg
      121 ctacgcaata cacatcagag tacatggatg tgaccgtttt caaggatggt tacaaatacg
      181 agcttcattt tgaaaaaggc atcaacatag gcggtctgaa aaaggaaaaa tacgactaca
      241 aacattcagg taccattcaa aaatggaagc ctgatcttga cgtttttacg gatataaaca
      301 tacctttgga gttttttcag actgtattaa aaaagcaggc cgttgtaaac gcaggtgtca
      361 agttcaaatt gtatgatgaa gaatcaaatc agcattttga atatatatat cccaaaggaa
      421 tagtcacata tgtggaggaa atcactcagg gcaaggaatt cacttccatt caatattacg
      481 agcatcaaac agcaggaagg gacagggcag acaagcccga gtacaaggtt aaaatgcaga
      541 ttgccttctg tttcaacaac catgtgaact tgattgaata ctaccacaac tccagctatc
      601 ttgaaaatgg cggttcgccg gagaaggctg tcaggaatgc ctttgttagt gagatagaca
      661 agtacatcaa aagtgaagga aaatatcaaa aggatgaatc aaaagtaacc tttgcggata
      721 ttcaggacag tttgattatt gtaaccaact ccttttcaac aatgactagt tacgagaacc
      781 aaaccaaaaa agctattaac aacaaattta ttcaggaagc aatgactgaa tttatcaaac
      841 aaaatcttga aatatatttc attgaaaaca aggccgaagc agataaaatt attgaacagg
      901 ttttggttaa caagagaagc cgtgaaatgg ctgaaaagca aaggattaac attaggaaga
      961 aactttcagg aaatattgac ataaccaaca gggtcaaaaa gtttgttgac tgcaggacga
     1021 gagacataga taaaagggag ttatatatag ttgagggtga ttctgctctc ggtagtgtaa
     1081 aactgggaag ggattcggaa tttcaggcaa taatgcccat aagaggtaag atccttaact
     1141 gtttaaaggc ggattacgga agaatattca agagtgacgt cattgtggat ctgataaagg
     1201 ttcttggatg tggcgtcgag gtaaaatcaa agtttaacaa ggatttgtcc acctttgatt
     1261 tggatgcttt aagatggagc aagataatca tatgtaccga tgcggacgtg gatggctatc
     1321 agataagaac actggtcctg gccatgattt acaggcttat gcccactctc ataaaagaag
     1381 gaaaggtgtt catagcagag tcgccgctat ttgagataaa ttgcaaaaac aagacgtatt
     1441 ttgcatacaa tgagcgagaa aagaacgaga taatttcaaa actggatgga aagtacacaa
     1501 tacagcgttc taagggactg ggtgagaatg aacccgaaat gatgtggcag accacaatga
     1561 atcctgagac ccgaaggctc attaaagtgg taatggagga tgtcgagagg acaaactact
     1621 actttgacat gcttcttggt gacaatttgg atgaaagaaa gaggcatatc gaggagaatg
     1681 gacatctgtt tatggatcat ctggatttaa tgtagaggaa ggtttaaatg gcagataata
     1741 agttatatat tgaacaaaag atatcagaaa cattggaatc aaattacatg ccctatgcca
     1801 tgagcgtcat tgtctcaagg gcaattcctg aaatagatgg atttaagcct tctcacagga
     1861 agcttttgta tacaatgtac aagatgggac tacttaacgg tcctaggaca aagtcggcca
     1921 acgttgttgg tgagacaatg aaactgaacc ctcatggcga catggctatt tatgaaactc
     1981 ttgtaaggat gacaaaaggc aatgaggctc tcttgcatcc ttacgttgat tcaaagggat
     2041 cctttggcaa agtttattca agggacatgg cttttgccgc agcaaggtat acggaagtaa
     2101 agcttgacaa gttatgtacc gagttgtttg gcgaattgga caaggatacg gtggactttg
     2161 tggacaacta cgacggcacc atgaaggagc ccacactgtt tcccgtcact tttcccacca
     2221 ttcttgtcaa ctcaaaccag ggacttgcgg ttggaatggc tacaaacata tgcagcttca
     2281 acctggttga aatatgcgag gcaacaagcg ccctgattga caatcctgag gagaatttgt
     2341 tgaagataat aaaggctcct gatttctcaa ctggtggcat tgtgatatat aacaagaagg
     2401 atatgaatag aattattgag acaggcaagg gccccataaa agtcagaagc aagtacaagt
     2461 atgacaagaa gaacaactgc atagacgtgt atgagattcc atacaccaca acagtggaag
     2521 ccataataga ttcagtatcc gatttggtca agaccaacaa gatcaaagaa gttgttgaca
     2581 tacgtgacga aacggatttg aagggtttga agcttaccat tgacataaag agaaacacaa
     2641 accctgacga tttaatgaac aagctgtaca tgatgacgcc actacaggat acgttttcat
     2701 gcaacttcaa cgtacttatt aaaggcaagc cacagctgtt gggagtccgc cagctgctgc
     2761 ttgagtggat tgaattcaga attgtctgca tgaaaaggaa gcttgctaat gaaattaaaa
     2821 caaaggaaaa caggcttcat ttgttaagag gtctcgaaaa gatactgctt gacattgaca
     2881 aggctataaa gattataagg gagacagaga ccgacgccct ggttatacct aaccttatgt
     2941 gggggttcgg tattgacgag attcaggcaa attttatagc cgagataaaa ctcagaaatc
     3001 tgaataagga atatatccta gacaaggtca gcgagattga gaaccttata aatgacataa
     3061 atgatttaaa ggatacacta ggaagcgata agaagattaa ggagcttatt aaaacaaagc
     3121 tccaggaagt tgccaagaaa catggtaaac ccagaagaac cgatttaatg catgaggacg
     3181 aagttgtggt tgtagaagaa gaggaaatga ttgacgatta caacttgaag gttttcctca
     3241 ctaacgacag ctacttcaag aagataccgc ttgtatcttt gcgttccgca tctgatcaga
     3301 agttgaagga tgaagacttc attgtccagg agatggagtg gcacaacaaa tcggacataa
     3361 tatttgtatc aaacaagcag gtggtgtaca agtgcaagct gcatgagttg ccggattgca
     3421 aggccagcag tctcggagag tatctgccaa atattcttga aatggatgat gatgagaaaa
     3481 tccattatat gatagtaacc gacaactatg caggttttat tctgtttgcg tatgagaatg
     3541 gaaaggtatc aaaaatacct ctaaacagct acgagacaaa aacaaacaga agaaagcttc
     3601 tcaacgccta cggttccagt gctcctttgg tggatgtcat attcacccat gaggatctgg
     3661 atatagctct tgtaagttct aatgacaggg cactggttat aaactcaagc atgattccgc
     3721 taaaaacaac aagaagctcc cagggtgttc aggtaatgag gcttaggaaa ggctgtgttc
     3781 tgcagagcat gatgaaggtt tccgagacgt ccttcaagga gccgaaagta tacagagtga
     3841 aaaacattcc tgcagcagga ccttttataa aagctcacga tagggagata cagcagatgt
     3901 ctttgcttaa tgagacagaa taaagagtgg cagggtttaa tccctgccac tttctaattc
     3961 atgttcttgt atacctgaat tctttctttt atatccggat atttcttaat tgtcttttct
     4021 gaaaacattg cattctcaat attctcagta gcagctttct catggttgat caaagcaagc
     4081 gttgcatcgt agagatttgc aaactctgga ttacgctgtg cttcgcatat gaaggactgt
     4141 cttggggagt tgatgtcttc tccgcttatc tggaacagat tgtacttgtc gcagtaagtg
     4201 cgtacagtct taagctgctc cattgtgttt cttgaaggca tgtaagtaac tgcattgaaa
     4261 tcaagctcgt cgataacatc aaaaagcaaa tcgatatagt cgtcttcaaa cttctgtgtc
     4321 tttttgtcac cagttacaga gtctcccacg tcaccaagat acgcataggc tgaaattgcc
     4381 ccaacttttc ttcccaattc aataacatcc cttacatcgg ggcattcatc ggttgcatcg
     4441 atatagaatt tttcaaccaa atcgctcttt aatgctccca agaggtcgta ttcataaaca
     4501 tcattgtctt cctgaagtag ataattctcg actttctttg ataggttcag attgagttca
     4561 ttttttagga aatcaacgac tttctgacct tttccaaact tgtccgtaat ctttttggaa
     4621 agagcaaaca gaaggtgtct ttccgtaacc gaaccattgt catggttcat ggaaaggggg
     4681 agtacatcct tgtcataatc catggatatt tcgtatggcg caaatatctt gtttatgttc
     4741 tctgtcattt ttctgtttct tgtatttctg tgagccgtgt aaggcttgaa gaactcaacg
     4801 acagtgtcta tgttctgatg gggtattcca tgcagtgcca tgtatgccac actcttctga
     4861 tcagggttgt ttattctaag gccgttgagt cgtgtctttg acatgtcggc tctgcattca
     4921 acgccgatgg tggtcatcat tcccacgatt ttgccagctt ctatgaattc ctttgcaccg
     4981 ctcaaggaat catggtccat tattccagct gtctttagac ctgccatata agccatccat
     5041 actgccttgg taggggagta gggtgagaag gaatatgtcg tatgaatatg gttgttgacg
     5101 tcgtttccca tgtctggttt cggagttttc ccatcttcaa ccatcttcat aagttctctt
     5161 aatgattcca gtcttgtttc ccttgatgga tggttcaagt tgttaacaag ttcttgcatt
     5221 ttattacctc cataaaatat atgtttattg aattctatca attatatttc ttgcaacgta
     5281 aataccgctg gctgcagcct aggacagacc tcttgttact cctgcgccgt ctccgcaagc
     5341 aaagaagttt ggtattggcg tttcaagcat ctcactcaat gatattctag cgctgtagaa
     5401 ttttacttct acaccatata acagtgtgtc atgatttgca gtacctggag caattttgtc
     5461 aagggcaaaa agcatctcaa ttatgttgtc aagatgtctt tttggcagag ccagactcaa
     5521 atcgccagga gttgcgttaa gggtcggcct tgtaaagctc tggctcatcc tgtgctcgtt
     5581 tgaacgaaca ccctttatga gatctccaaa tctttggacc agaacacctc cgcccagcat
     5641 gttggacagg gatgcaattc tctttccata ctgataaggc tcattaaaag gttttgtaaa
     5701 cctgttgctg acaagcagtg caaagttggt gttctcgctt ctcagtttct cgtcactgta
     5761 gctgtgtccg tttacagtga taagaccgtc gacgttttca gttactacat gaccgtacgg
     5821 attcatgcag aaggttctta cgttgtcgcc gtactgcttc gttctgtaaa caagctttgc
     5881 ctcgtataca acatttgtta tatcttcgaa aacctttgca ggaagctcaa ctctgacacc
     5941 aatgtcaacc tggttgttga tgagttttat tcccagtttc tctgcctggg aagcaaacca
     6001 ttccgcgcct gatctgccag gggaggcaat caggtacttg caggtaaccg tctcgccatc
     6061 ggacaactga aggttgaaag tgccgtcagg atttacgctg atggattcga cggatgtatt
     6121 gcatttaatg tttatcttct cgacgaggta gtcgtacatg ctcttaagta ttcttaggtt
     6181 gttttcagtg cccagatgct ttaccgaagc cattagcagg tgcaagtcgt tatgcaatgc
     6241 cttcttttct agttctcgtg ccttgtcaga gtatgttgag tatctttcgg tggttgcacc
     6301 gaaggacaca ttgatgctgt ctgcatattc aatgagctcc attacctttt cccttggaag
     6361 gtaatcgtga agccatcctc caaattcagt agtgaaattg aatttaccat ccgaaaaggc
     6421 gccagcacct ccgaagccgt tcatgatgcc gcagtttgca caggaaatgc agtttcttgt
     6481 tatgttctgc acaattgggc attttctttc ataaataggt ttgccttgtt ctataataag
     6541 tatgtcaagg tttggtatgt tcttgtttaa ttcatatgaa gccattattc cagcagttcc
     6601 tccgccgatg attgccacat cacatttcaa tttgttttac ctccgatata nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttcctccgc cgatgattgc cacatcacat
     6781 ttcaatttgt tttacctccg atatattaag atgtatgtta aacataacac cttattatat
     6841 cccataaaaa agatttagta aataccattg caatttacaa cctcgtgtag taaaataatt
     6901 tagtctgaca gagcaatgtc agtattatgt ttatacacat attaatttgg gggttaaatc
     6961 tatgtacaag aaaatgattt tggtaatggt agcaattgtc atggcactgt ctgtcttttc
     7021 cggctgtaca ggcgacaatg atggcgatgg cgacgctgtg agcaagttga aaattggtgt
     7081 tgtatacatt ggaaacattt cagaacctgt tggattttca actgagcact acaagggcat
     7141 catggctgct gccaagtctc ttggcataaa ggaaagccag atacttcaga gagaagaggt
     7201 ctcagacaac gacgagagct gcaaggaagt atttgagagc cttattgcca atggatgcaa
     7261 cgtgattatc ggtacaagct atggatacgg cacgtatatg aatgatctgg ccaacgatcc
     7321 tgcatacaag gatataattt tcatgcactg ctcgggagaa tacaagaatg atgtaaactt
     7381 ctcaaacttc ttcggaagaa tgtatcaggc aagatatctc actggtattg tagcaggttt
     7441 gaagactgag acaaacaaga taggatatgt ggcagccgtg ggaatcccgg aagttattcg
     7501 tggaatcaat gcattcacac tgggggtcaa aagcgtgaat ccagatgcag aagttattgt
     7561 aaggtggaca aatacctggg gtaacccaag cattgaaaaa tccactgcag aaacacttat
     7621 cagtgaagga tgcgacgtta tcacccagca tcaggatacc gacgctgcac agattgcagc
     7681 cagagaaaac aaagtgtggg ctatcggata ccatagcgat atggctgttt cctccaagga
     7741 gtacaacctc accagtgcta tctggaatct tgaagcatat tacgaacaac agttcacgga
     7801 tataatcaat ggcgaatgga agccaggcgt tgcatactgg ggtggaatca aggaaggcgt
     7861 tgttgacatt gcaccacttt cagacaatgt tgcagaagga acacaagcta aagttgacga
     7921 agttcttgca aaattcaaag atggtagctt tgatgtcttt gcaaactacg aaattaagga
     7981 tcaggatgga aacgtcaagg ttacagctga caccaagctt acagacgaag aacttctgag
     8041 catgatgtgg tttgtagaag gtgttgtagg aaccattgat taataaaaag tcaatatgag
     8101 ggacatgcaa tgcaggattg tatcttgcat tgctttttca attttttaat tatatgtgat
     8161 aaaggtgatt cagtgaacaa tgtgctgttt gagctgcgtg gaataacaaa actatttgga
     8221 aatgttgtag caaacaagaa tattgatttt tccgtaagag aaggagaaat acatgctctg
     8281 ctgggagaga acggatctgg aaagacaacc ttgatgaaca tactgtcggg aatatatact
     8341 ccggatagcg gcacaattat tataaatggt gaggaagtaa ccataaaatc tcctgaagat
     8401 gcaatcaagc tcggaatagg aatgatacac cagcacttta agctggttga tgtgttttct
     8461 gccaaagaga acataatcat ggggcagtcc ggttccataa ggcagaatca aaagaatctt
     8521 acaagagaaa tcgaagagct gagcaacagg tttggcctta atataaattt ggacaagaaa
     8581 atttatcaga tgtccgtaag tgaaaagcag actgttgaga taatgaaggt gctgttcagg
     8641 aaatccaaaa ttcttatcct ggatgagcca actgctgttc ttactccaca ggaaattgac
     8701 aaactttttg aaattcttag ggaactcagg gatgatggct gtgcaatcgt cataattacg
     8761 cacaagctga atgaggttat ggagattagc gatagagtaa ccatcctgag aaaaggggag
     8821 agtgttgcta ctgttgagac aaaacaaaca aatgccaagg aacttactga actcatggtg
     8881 ggtcatagtg ttgatttaag tatagaaaga cctgttgttg aggacaagac aaaagatgtt
     8941 ctacaggtgg acaaccttac aattttcgat gaagatggag taaaggtaat tgacggcatg
     9001 tcctttgcgc tggatggcgg tgaaatccta ggtgttgccg gtgttgcagg atcagggcag
     9061 aaggaactat gcgaggcgat tgcaggtctg catcctgttg ccgaaggatc tatagaattc
     9121 aaggggcgta acatagttgg attaaatccg aatgagatta ttaatttggg agtaagtatg
     9181 agctttatcc ctgaggacag acttggcatg gggcttgtgg ccaacatgg
//
LOCUS       DAONVY010000018         9094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_5859_length_9094_cov_3.899989, whole genome shotgun sequence.
ACCESSION   DAONVY010000018 DAONVY010000000
VERSION     DAONVY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5859_length_9094_cov_3.899989"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PLI11_01010"
     CDS             complement(<1..1020)
                     /locus_tag="PLI11_01010"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPZ51473.1"
                     /translation="MKKKIAFFDTKAYDKHFFDMYNNGEFEIQYFESKLNKHTAEMTK
                     GYDAVCAFVNDDINADVIDVLYENGIGLVALRCAGYNNVDIKHAYKRVHVTRVPSYSP
                     NAVAEHAMALLLTLNRKIHRAYNRTREHNFSLEGLTGFDLYGKTVGVIGTGKIGRAFA
                     NICKGFGMKVVAYDLYPAPDADFEYKTLDEIFKEADIISLHCPLNEETRHMINDESLG
                     KMKDGVAIINTSRGQLIDTQALIRAIKCGKVGMAGLDVYEEESEFFYEDHSNNILQDD
                     ALVTLISMPNVIVTSHQAFLTKEALQAIAQTTLKNMRDYFDDKVLVNEICYRCEEYGI
                     CDMGHT"
     gene            complement(1091..1372)
                     /locus_tag="PLI11_01015"
     CDS             complement(1091..1372)
                     /locus_tag="PLI11_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPZ51474.1"
                     /translation="MIIGSVIVKIYVPSVHSLKEKRTIVKSLVTKVKNKFNVSIAEVS
                     ENDTHQTIVLGFACVTISTKHANSIIDTVINFIENNTEGDIVDIVREIL"
     gene            1512..2453
                     /locus_tag="PLI11_01020"
     CDS             1512..2453
                     /locus_tag="PLI11_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPZ51475.1"
                     /translation="MKLLFKENIFEKNTPSCHASTLAKTDDGFVAAWFGGTEEGKDDV
                     RIYVSVRINNKWEKPLCVSLDDGLPHWNPVLFNNGNELLLFYKSGKTIASWMTFITKS
                     KDYGRTWTLPERLCNDDIGGRGPVKNKPIYLSNGWLVAPASTEIGQWKCFVDISKDRG
                     KTWTKGNIVPSPDEKTNLIQPSIWEDSSGLHMLMRSNRQRIFRSNSKDNGLTWCKAYP
                     INIPNNNSGLDLVKIKSDMLVLACNPVEAGRSPLSLLASYDKGLTFKHELTLQDGEGE
                     FSYPSVIAEGNRVYGVYTYKRTLIRFFCVESQALPKI"
     gene            2587..3609
                     /gene="aroF"
                     /locus_tag="PLI11_01025"
     CDS             2587..3609
                     /gene="aroF"
                     /locus_tag="PLI11_01025"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPZ51476.1"
                     /translation="MVLILRKNAEEKQLYNLIEWLKNQGLDVHLSKGRYQTIIGLIGD
                     VSAIDIELLKGLNIVESVMRISEPYKNANRKFHPDDTVLDVCGIKIGGGHFNVIAGPC
                     SVESTEQIIEVAKSVKKSGATLLRGGAFKPRTSPYAFQGHRDKGIKMLLEAKKETGLP
                     IVTEIMDVGSLPLFEDVDIIQVGARNMQNFELLKELGMTRKPILLKRGLANTLNEFLM
                     SAEYIMAGGNNNVILCERGIRTFETSTRNTLDIAAVPLLKQYSHLPVLVDPSHASGIA
                     PIVPPLSLAAIAAGADGIMVEVHNDPEKALSDGAQSLNPAQFDDLMQDVKKMFTIFTD
                     RKLVEI"
     gene            3621..4445
                     /locus_tag="PLI11_01030"
     CDS             3621..4445
                     /locus_tag="PLI11_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPZ51477.1"
                     /translation="MNIGIIGLGLIGASLAGAFNSKTSHAVFGYDLDEKTIKKALKLG
                     FIDKVLDNDLLTECDIVVLALYPKDVVKFVLKNRDSFKRGAIVVDCAGVKRSVCEEIW
                     ANTKDNNFTFVGGHPMAGRQFSGIDYASPTLFNKASMILVPEENTECPKVLAELFEQI
                     GCYITITTKEEHDAMIAYTSQLAHVVSNAYVKSPASEKHKGFSAGSYMDLTRVAYLNP
                     DMWTELFLENADNLTNEIDFLIDNLVKINKAIKERDADTIRQLLKEGADIKKRVDM"
     gene            4446..5498
                     /gene="aroB"
                     /locus_tag="PLI11_01035"
     CDS             4446..5498
                     /gene="aroB"
                     /locus_tag="PLI11_01035"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPZ51478.1"
                     /translation="MAIVTVNASKSYDVVISPYILRNTGDILKDKFGVKTNCIITDET
                     VDSMYSPVVVESLEKSGHKVVKYVFPVGENSKGAENFVNILNFLASNKLTRSDIIIAL
                     GGGIPGDVAGFAAATYLRGVKFVQIPTTLLACVDSSVGGKTAINLDEGKNLAGAFYQP
                     DLVLCDYSTLETLPDHVFSDGVAEVVKYGVIKDEHLFNLAEMNLLKDKMEEVIERCVS
                     IKRDIVNEDERDMGVRQLLNYGHTYGHAIEKASNYTLSHGSAVAIGMCMASKAAYKLG
                     FSDEDITDRLVETLRLYNLPTQCPYDVETLINAALSDKKRQGEKINLVLPKRIGECFL
                     QTIDVKDLEKYFEAGM"
     gene            5500..6795
                     /gene="aroA"
                     /locus_tag="PLI11_01040"
     CDS             5500..6795
                     /gene="aroA"
                     /locus_tag="PLI11_01040"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPZ51479.1"
                     /translation="MDVRIKPGKLSGEIEAISSKSDAHRILIGSLLSKNETNLHVNIM
                     SEDIKATIDCIREAGCTVDYAHNMLKIKPVPIDVDKEYHFDCNESGSTLRFFIPIAAA
                     LGINATFTGRGRLPERPLSPLVEELEAHGVRIKRPDDAYLPLVLEGKLTPGIFKIAGN
                     ISSQFVTGLLFALPLLQEESKLIITPPVESRPYIDMTLNTLRKYGIKVQETRENENTC
                     FYVTGGQKYTSPKHIEAEGDWSNAAFWFVAGAMSEEGMVIKGVNERSLQGDKYIVEHL
                     IRVGAKVEKIDDGYKVTRNNLLARDVDAAKIPDLVPIIATLASVSKGTTRIYNAYRLR
                     IKESDRLSTVAQNLRNLGADITEFEDSLVINGKEMLEGGIADSYNDHRIAMSMAIAAG
                     ACKNDVIIKNAEAVNKSYPTFFDDFKRRKGVFDVIELRE"
     gene            6773..7864
                     /gene="aroC"
                     /locus_tag="PLI11_01045"
     CDS             6773..7864
                     /gene="aroC"
                     /locus_tag="PLI11_01045"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPZ51480.1"
                     /translation="MSSSYGNRIKVQIFGQSHSKAIGVVMDGIPAGIEIDMDKLHGFM
                     ERRAPGRNIYSTQRREKDMPEILSGIVDNVTVGAPICAVIHNTDTRSKDYDNLRDMPR
                     PGHADFPAYVKYQGANDIRGGGHFSGRLTAPLCFAGNICMQMLEKQGITIGAHIARIQ
                     DVMDKPFDPVSIGREELESIKEKEFPVLDDEKGALMRERIESARMSMDSVGGTIECAV
                     VGLPAGIGEPIFDGIENNIAKVTFGIPAVKGIEFGAGFMVSEMKGSENNDEFYYDDEG
                     TIKTYTNNHGGILGGISSGMPIIYRVAIKPTSSIAQKQRSISLSRKENTILEIKGRHD
                     PCIVPRAVPVVEAACAIALLDLLIYERAE"
     gene            7866..9008
                     /locus_tag="PLI11_01050"
     CDS             7866..9008
                     /locus_tag="PLI11_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPZ51481.1"
                     /translation="MDINELRDKIDSIDSEILKLFTQRMKISLEIAKYKKENNLPIQD
                     KKRERELIRKVDENAETGLEIFSRMLFNTLMDLSRSYQYSCVNQATNLAKIIADRSKM
                     TPRQFPEKAVVAVQGDEGSYGQFACDKLFKIPTIMYFNSFEAVFQAVEKGLCHYGVLP
                     LENSTAGSVNQVYDLMGKYKFFIVRSARLRVDHTLLAKRGVKLSDIKEVVSHEQALNQ
                     CSDFLATLKGVKVTKIDNTALAAKYVSESGRDDIAAISSKDCADIYNLAVLSYNIQNN
                     DNNYTRFICISKDLEIYPGADRTSITLTIPHKPGALYHVLARFYALGLNLAKIESRPI
                     PGRDFEFLFYFDIDTSVYTPELFMLISELECGIEQFNYLGSYKEIL"
BASE COUNT         2888 a         1766 c         1969 g         2471 t
ORIGIN      
        1 tgtgtgcccc atgtcgcata ttccgtactc ttcacatctg tagcaaattt cattgacaag
       61 aaccttgtcg tcaaaatagt cgcgcatgtt ctttagcgtg gtttgtgcaa ttgcctgcaa
      121 tgcttccttg gtcaagaacg cctgatggga agtaacaatt acatttggca tggatatgag
      181 agtgaccagt gcgtcatcct gaagtatgtt attggaatgg tcctcataga aaaactctga
      241 ttcttcctcg tatacatcga gaccagccat tcccactttc ccacatttta ttgctcttat
      301 caatgcttgt gtatctataa gttgtcctct tgaagtgttg ataattgcta ctccgtcctt
      361 catcttgccc agagactcat catttatcat atgtcttgtc tcttcgttta gcgggcagtg
      421 aagtgatatg atatccgcct ctttaaatat ttcatccagg gtcttgtact caaaatccgc
      481 atctggagcc ggatacaggt catatgcaac aaccttcatt ccaaacccct tgcatatgtt
      541 ggcaaaggct ctgcctatct tgcctgtacc aataacgcct acagtctttc cgtacaaatc
      601 aaagcctgta agaccttcaa gactgaaatt atgttcccta gtcctgttgt atgccctgtg
      661 tattttgcgg ttcaacgtaa gaagaagcgc catagcatgt tccgcaactg cattaggaga
      721 atatgaaggt acacgtgtta catgtaccct tttgtatgca tgcttaatgt cgacattgtt
      781 gtaccctgca caccttaacg caacaagacc aatgccgttt tcataaagga cgtcgatgac
      841 gtccgcattt atatcatcat tgacaaaggc acataccgca tcgtatccct tcgtcatctc
      901 ggcagtatgc ttgttcagtt tgctttcaaa gtattgtatc tcaaattctc cgttattgta
      961 catatcgaag aaatgtttat cgtatgcttt tgtatcaaaa aatgctattt tctttttcat
     1021 cgcatttcct ccaatcaata aaagttagac tactcttaca ttatatcatt aatataacaa
     1081 aaaataaaat ttataggatt tcacgaacaa tatcgactat atcaccctct gtattgtttt
     1141 caatgaaatt gataacggta tctataatac tgtttgcatg ctttgttgat atagtaacgc
     1201 aggcaaatcc caatacaata gtctggtggg tatcgttttc acttacttca gcaattgaaa
     1261 cgttaaactt gtttttaacc ttggttacga gactctttac aatcgttctt ttctccttca
     1321 gtgaatggac acttggtaca tatattttca caataacact gcctattatc acaaaaatca
     1381 cccttataaa aaacactata ctaatattat atatttattt tccctttcgg tcatctgatt
     1441 taattactcg gaccaaatag ttgtgtttct gtggtatatt attgatatca attataggga
     1501 ggcctctcaa ggtgaaactt ctgtttaagg aaaacatttt tgaaaagaat acaccgtcct
     1561 gccatgcatc aactttggca aaaactgatg atggttttgt ggcagcatgg tttggcggta
     1621 ctgaagaagg aaaagatgat gtaagaatat atgtctcagt taggataaac aacaaatggg
     1681 aaaagccact ttgcgtttct ctagatgatg ggctgccaca ttggaatcct gttttgttca
     1741 acaacggcaa cgagctcctt ttgttttaca aatcaggtaa aacaattgct tcatggatga
     1801 cctttataac caaatcaaaa gattacggga gaacatggac cttgccagaa agactctgca
     1861 atgatgatat tggcgggaga ggtcctgtaa aaaacaagcc catttacctt agcaacggat
     1921 ggctggttgc tcctgcttct acagaaattg gtcagtggaa gtgttttgtt gacatatcca
     1981 aagacagggg aaagacgtgg acaaagggca atattgttcc ttctccagat gaaaagacaa
     2041 atcttataca gccttcaata tgggaagatt ccagcgggct gcacatgctt atgcgttcca
     2101 acaggcaaag aattttcaga agcaattcca aagacaatgg ccttacctgg tgcaaggcat
     2161 atccaattaa cataccgaac aacaacagcg gtttggattt ggtaaaaata aaaagtgata
     2221 tgctggtact tgcatgcaat cctgttgaag caggcagatc tcctttgtca ttgcttgcaa
     2281 gttatgataa agggttaact ttcaagcatg agttaactct gcaggatgga gagggagagt
     2341 tttcctaccc ttcagtcata gccgagggca atagagtgta cggtgtgtat acatacaaaa
     2401 gaacgcttat cagattcttt tgtgtagaaa gtcaagctct tccaaaaata taattgacac
     2461 atgcttttat catgtggtag aatgatattt aaactcaaaa cttccgcaga aatcaaagcg
     2521 gttaatattt ccccaaatct gttcaccaga acagattatt tctcatatag aaaatggtgt
     2581 atgtcaatgg tattaatttt aagaaaaaac gcagaagaaa aacagctata caatttaata
     2641 gagtggctta aaaaccaggg tctcgacgtg catttgtcaa agggtagata ccagacaatc
     2701 ataggcctca ttggtgatgt aagtgcaata gatattgaac ttctaaaggg cctaaatatt
     2761 gtggaaagcg tcatgcgcat ttccgagccc tacaaaaatg ccaacagaaa gtttcatccc
     2821 gacgatactg tcctggatgt atgcggtata aaaattggcg gaggacattt caatgttatt
     2881 gcaggtcctt gctccgttga atcaaccgag cagattattg aagttgcaaa gtcagtaaaa
     2941 aaatcaggag caacactttt gcggggcggt gctttcaaac ctcgcacatc accttatgct
     3001 ttccaaggac acagagacaa gggtataaaa atgctccttg aagcaaaaaa ggaaaccggc
     3061 ctccccatag taactgagat tatggatgta ggaagccttc cattgtttga ggatgtggac
     3121 ataattcagg ttggcgcacg caatatgcag aactttgaac tattgaagga attgggaatg
     3181 acaagaaaac cgattcttct aaaacgaggt cttgcaaaca cactcaacga gtttttaatg
     3241 tccgccgaat acattatggc aggtggaaac aataacgtta tcttgtgtga aagagggatt
     3301 aggacctttg agacgtcaac aagaaacacc cttgatattg cagctgttcc gctactgaaa
     3361 cagtactccc acctacccgt actagtcgac ccgtcccacg catctggaat tgcaccaata
     3421 gtgcctcctc tttctcttgc agcaattgct gcaggcgcag acggaataat ggttgaagtt
     3481 cacaatgatc ctgagaaggc tttaagtgat ggtgctcagt cattaaatcc agcacagttt
     3541 gatgatttga tgcaggacgt aaagaagatg tttacgattt tcactgatag aaaactggta
     3601 gaaatatgag aggaaaagag atgaatatcg ggataatcgg cctcggcttg ataggtgcat
     3661 ccctcgccgg ggcattcaat tccaagacaa gccatgccgt ttttggctat gatttggatg
     3721 agaaaacaat caaaaaagcg ctcaaactgg gcttcatcga caaagtactg gataatgacc
     3781 ttcttaccga atgcgacata gtagtattgg ccttgtatcc gaaagatgtg gtaaagtttg
     3841 tactgaagaa tagggacagc ttcaaaagag gggcaattgt tgtagactgt gcaggtgtta
     3901 aaagatccgt atgcgaggaa atatgggcga acacaaaaga caataacttt acttttgtag
     3961 gtggacatcc tatggcagga agacagttct ctggaataga ttacgcctct cctacactgt
     4021 ttaacaaagc ctccatgata ctggtacctg aagaaaacac agagtgtccg aaagtacttg
     4081 ctgagctttt tgaacaaatt ggctgctata tcacaataac tacaaaagaa gaacatgacg
     4141 caatgattgc atatacatcg cagcttgctc atgtggtgtc aaacgcttat gtaaaaagtc
     4201 ctgcttcgga aaaacacaaa ggtttttcgg caggcagcta catggatttg acaagggttg
     4261 cataccttaa tccggatatg tggacggagc tgtttcttga aaatgctgac aatttgacta
     4321 acgaaatcga ttttttaatc gataaccttg taaagataaa caaggctatc aaggaaagag
     4381 atgccgacac gatcaggcag cttcttaagg aaggagcaga cataaagaaa agggtggata
     4441 tgtaaatggc tattgttaca gtaaatgcat caaaatcata cgatgttgtt atatctccat
     4501 atattttgag aaataccggc gatattctaa aagacaaatt tggagtaaaa accaattgca
     4561 taattacaga cgaaactgtg gatagtatgt attcgcccgt tgttgttgaa tctctggaaa
     4621 agtccggcca caaggtggta aaatatgtat ttccagttgg tgaaaactca aaaggtgcag
     4681 aaaactttgt aaatatactt aactttcttg cctcaaacaa actaacaaga tcagacatta
     4741 tcattgcgct gggaggcgga atcccaggag atgtggctgg ctttgccgca gctacatatc
     4801 tcagaggagt caagtttgtg cagataccca ccaccctcct tgcttgcgta gattcttccg
     4861 tcggaggcaa gaccgcaatt aatctggatg aagggaaaaa cctagcagga gcattttatc
     4921 agccagacct tgtgctttgt gattattcaa ccctggaaac actgcctgac catgtcttct
     4981 cagatggtgt tgctgaggtt gtcaagtatg gagttataaa agatgagcat ctttttaatc
     5041 tggctgaaat gaatttgctt aaggataaaa tggaggaagt tatagaacga tgtgtgtcca
     5101 taaaaagaga tattgtaaat gaagatgaac gtgatatggg agtacggcag cttttaaact
     5161 atggacacac ttacggacac gccatcgaga aggcaagcaa ttacactttg agccacggtt
     5221 ccgccgttgc aataggcatg tgtatggcct ccaaagcggc atacaagtta ggtttctcag
     5281 atgaagacat aactgacaga cttgtcgaaa ctctaagact atacaatctt cctacacaat
     5341 gtccttatga tgttgaaaca ttaatcaatg ctgccttgtc agacaagaaa agacagggag
     5401 aaaagattaa tctggtattg ccgaaaagaa ttggtgaatg ctttttgcag acaatagatg
     5461 tgaaggactt ggaaaaatat tttgaagcag gtatgtagta tggatgtacg gattaaacca
     5521 ggaaaactta gtggtgaaat tgaggcaatt tcatcaaaat cagatgcgca tcgcattctg
     5581 attggttctt tattgtcaaa aaatgagaca aatcttcatg taaacataat gtcggaagat
     5641 attaaggcga ccattgactg cataagggaa gcaggctgca cagtcgatta cgcacacaac
     5701 atgcttaaaa tcaaaccagt gccaattgat gttgacaagg aatatcactt tgactgcaac
     5761 gaaagcggct ctactttgag attctttatt ccgattgctg cagcactggg cattaatgcc
     5821 acctttacag gcagaggaag acttccagaa agaccccttt cccctcttgt ggaggagctt
     5881 gaagcccatg gagtcaggat taagaggcct gacgatgcgt accttcctct tgtattggaa
     5941 gggaaattaa cacctggcat ctttaaaatt gctggaaaca taagctccca gtttgtcaca
     6001 ggtcttttgt ttgcgctccc tcttttacag gaagaaagca agttgataat tacgccgccg
     6061 gtagaatccc ggccctatat tgacatgacc cttaacacgt taagaaaata tggaataaag
     6121 gtgcaggaaa caagagaaaa tgaaaacacc tgcttttatg taactggcgg acaaaaatac
     6181 acttcaccga aacatattga agctgaaggc gattggtcca atgcagcatt ctggtttgtg
     6241 gcaggagcca tgagtgagga aggcatggtt attaaagggg tcaacgaacg atctcttcag
     6301 ggagacaaat atattgtgga gcatctaatc cgtgttggtg caaaggtcga aaagattgat
     6361 gatggttaca aggttacaag aaacaacctg cttgcaaggg atgtagatgc tgcaaaaatt
     6421 ccagacttgg taccaattat tgcaacactt gcctctgttt ccaaaggcac aaccagaata
     6481 tataacgcat acagacttag aataaaggaa agcgatagat tgagtactgt tgcacaaaac
     6541 ctaagaaatc tgggtgcaga cattacagaa tttgaagaca gtttagttat taatggcaaa
     6601 gaaatgcttg aaggcggtat tgcagacagt tataatgatc ataggattgc catgtctatg
     6661 gcaattgctg caggtgcatg caaaaatgat gtgattatta aaaacgcaga agcggtaaac
     6721 aaatcatatc ctacattctt tgatgatttc aagagaagaa agggtgtttt cgatgtcatc
     6781 gagctacggg aatagaataa aagttcagat atttggacag tcccattcaa aagccattgg
     6841 tgttgtcatg gacggcatac cagcaggcat tgaaatagac atggataaat tgcatggatt
     6901 tatggaaaga cgagctcctg gcagaaatat ttacagtact cagagaagag aaaaggacat
     6961 gcctgaaata ctctctggaa ttgtagataa tgttacagtg ggtgctccaa tttgtgcagt
     7021 tatccacaat accgacacaa ggtccaagga ttacgataac ttaagggaca tgccaagacc
     7081 tggccatgca gacttccctg catatgtaaa ataccaggga gccaacgaca taagaggagg
     7141 aggccatttc tcaggcaggc tgactgcgcc tctttgcttt gcggggaaca tatgcatgca
     7201 gatgttggag aaacagggca taacaatagg tgctcatatt gccagaattc aggatgtaat
     7261 ggacaaacct tttgaccctg tcagtattgg cagggaggaa cttgaaagca taaaggaaaa
     7321 ggagtttccc gtactggatg atgaaaaagg cgcgcttatg agagaaagga tagaaagtgc
     7381 cagaatgtcc atggattcag taggcggaac aattgaatgt gctgtagtgg gattgcctgc
     7441 aggtattgga gaacctatat ttgacggaat agaaaacaac atagcgaaag taacctttgg
     7501 aattccagcc gtcaaaggca ttgagtttgg tgcaggtttc atggtttctg aaatgaaagg
     7561 ctccgaaaac aacgatgaat tctattacga cgatgaaggc acaatcaaaa catatacaaa
     7621 caaccatggg ggtattcttg gaggaatatc ttccggcatg ccaattatat acagagtcgc
     7681 aatcaagcct acctcctcca ttgcacagaa gcaaaggtcg ataagtttat ccagaaagga
     7741 aaacaccatt cttgaaataa aaggacgtca cgatccttgt attgtaccaa gagcggtgcc
     7801 tgttgttgaa gcggcatgtg cgatagcctt attggattta ttaatatatg aaagggcgga
     7861 atgaaatgga tattaatgaa ttaagagaca agattgatag tatagacagc gagattttga
     7921 aactgtttac ccagaggatg aagatttccc tggaaattgc caaatacaaa aaggaaaaca
     7981 accttcccat acaggacaag aaaagagaaa gagaactaat acgcaaagtg gatgaaaatg
     8041 ccgaaactgg ccttgagata ttcagcagaa tgctgttcaa cacccttatg gacctaagcc
     8101 gctcatatca gtactcctgt gtaaaccaag ctaccaatct ggcaaaaata attgcggaca
     8161 gatcaaagat gactccacgt cagttccctg aaaaggctgt tgttgcagtc cagggcgatg
     8221 aaggctcata tggacagttt gcctgcgaca agctgtttaa aataccaact attatgtatt
     8281 tcaacagctt tgaagcggta tttcaagctg tcgagaaagg tctttgccat tatggagttc
     8341 tgccacttga aaacagtacc gcaggctcgg tcaatcaggt ctatgacctt atgggcaagt
     8401 acaaattctt tatcgttaga agcgcccgcc tcagggttga tcatacactt ctggctaaaa
     8461 gaggagtaaa attgtcagat atcaaggagg ttgtgtccca tgaacaggcg ctgaaccagt
     8521 gcagcgattt tcttgctacc ttgaagggag taaaggtaac gaagatagat aataccgccc
     8581 ttgctgcaaa gtatgtatcc gagtctggaa gggatgacat agctgcaatt tcatcaaagg
     8641 attgtgctga catatacaat cttgctgttt tgtcctacaa catacagaac aatgacaaca
     8701 actatacaag attcatatgc atatccaagg atcttgagat atatcccgga gcagacagaa
     8761 ccagcataac ccttacaata ccccacaaac caggtgctct ctaccatgtg ctggcaaggt
     8821 tttacgccct tggcctgaat cttgcaaaaa ttgagtcaag gccaataccg ggcagagact
     8881 ttgagttcct gttctacttc gacatagaca catcagtata cacaccggag ttgttcatgc
     8941 tcataagcga gcttgagtgc ggtatagagc agttcaacta tcttggaagc tacaaagaga
     9001 tattatagca caaaaagggg ctgatgcaga ttgcaacagg ccctttattc aacacatgta
     9061 atttcctcaa tcaaaattgt cctctgccct tttt
//
LOCUS       DAONVY010000019         8937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_5976_length_8937_cov_4.013173, whole genome shotgun sequence.
ACCESSION   DAONVY010000019 DAONVY010000000
VERSION     DAONVY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5976_length_8937_cov_4.013173"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PLI11_01055"
     CDS             complement(<1..1110)
                     /locus_tag="PLI11_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPZ51482.1"
                     /translation="MNPILPRPFFIADGEAHVMPDGRLYLYGSTDISGKKEYCGHEYH
                     VFSTEDETLTNWTYHGISFKNTKEEPGIYWSKGTPLYAPDAIHKDGKYYLYVCGQDNF
                     EAVAVSDKPYGPFGDAKPVKGADGDSIDPAVFVDDDGQAYYLWGQFNLRGAKLNDDMS
                     TLDMDSLQTAILTEQEHGFHEGACMRKRNGIYYIVYTDISRGKATCLSYAMSDKPLGP
                     YKKGGVIIDNMYCDPQTWNNHGSIEEFKGQWYIFYHRSSQNSFTSRRMCVEKIYFNED
                     GTINEVEMTSMGAGGPIDAFKEIDASIACRVKGNIYIAPYFDSKEILVNCGGGNWIQD
                     WAEYKYLDFKDGVRRFCIKTKGKGRILVKTNDNLLL"
     gene            complement(1144..2310)
                     /locus_tag="PLI11_01060"
     CDS             complement(1144..2310)
                     /locus_tag="PLI11_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPZ51483.1"
                     /translation="MFSLLLVIIYIAFISLGLPDSLLGSAWPVMYHELDVPMSYAGIV
                     TMIIAGGTIVSSLMSDRVTRRFGTYFVTSVSVLMTAVALFGFSITKSFILLCIFAIPY
                     GLGAGAVDAALNNYVALYYNSRHMNHLHCFWGVGATISPYIMGFCLTRGFGYSKGYLT
                     VSVIQIVIASFLFTSFPLWRKNGQAMKDKAANGRHITIKEAVGIKGVKLILVGFFSYI
                     ALESTAGLWASSYLVECRNVNAETAATFASMFFLGITIGRFISGFVSNRVGDRLMIRS
                     GIIVCMFGVLMIALPFSSVLSLVGLVVAGLGCAPIYPAIIHSTPSNFGKENSQAIIGI
                     QMASAYVGSTFMPPLFGLIADYVNIGFYPFYLAVFAVLMLLMTEKLNKVVNNLV"
     gene            complement(2360..3052)
                     /locus_tag="PLI11_01065"
     CDS             complement(2360..3052)
                     /locus_tag="PLI11_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51484.1"
                     /translation="MDNEFDENVVIGEETEENEIKTGIKESRYTWVISIFVLLVFLCV
                     FLLFKNEDDNEILSNSPTQSTNQTTPEPSFKNIITERYLLAMDEYSSLIMKGEYDYYN
                     DYARLEIYFDNITSQPLVLWTPKWMYYEKPYSAIFHKDECYVLTRGAYADYEEMMRTF
                     RPSVKEPIYLWRLNELGNWEGVVLDFGIDTLEKCSYFNFETLRGSVSDKVVISLFDKY
                     GKRINSIPIPRI"
     gene            complement(3165..3728)
                     /locus_tag="PLI11_01070"
     CDS             complement(3165..3728)
                     /locus_tag="PLI11_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPZ51485.1"
                     /translation="MEKEKRMFQKTKHVPNMLTFSNMAVGVLVILTMMRSNSVTGTRL
                     ACLLIYLAVIFDSLDGFLARHLNATSELGRQLDSFADFVTFGVAPISIFMSRIEEPSW
                     YVLFVLVMYPLAGAFRLARYNLQDDYRFFKGLPITAAGFITASTLFIHSFVSQEFSNM
                     FVLVFVLQTILLSFLMVGSFRVKRILH"
     gene            complement(3682..4677)
                     /locus_tag="PLI11_01075"
     CDS             complement(3682..4677)
                     /locus_tag="PLI11_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPZ51486.1"
                     /translation="MRVVRRTGYVVLLFLFVILILTFDLKSFVDSIRKVPVLGIIGLL
                     LIQVVSQLLVNYQWCRIGKVMNKENDFFKMLYINARGMIMESITPGVKLGGEVTRAYL
                     LKSEMNYSYQEAATLVTIQKMVSFFSFFVINLFAFAHLSGKVAIFQGFVRVVVFLFLL
                     TFLGLLVIMFVGTNILENKVKKRKPKRKASIALHKYMMVLLRNIKVLKSIKGEMYKQF
                     LLSFAIWIIYPLKMILLVNLFTAEYNWVFLTEITFISYMVGMIPLMPGGLGGFETAMT
                     SLLVLMQIEKNEALAITLIFRFITFWFVILLSLLYIGGWKIGERKKNVPKNQTCS"
     gene            complement(4691..5251)
                     /locus_tag="PLI11_01080"
     CDS             complement(4691..5251)
                     /locus_tag="PLI11_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51487.1"
                     /translation="MAGLIQRTTKKLECLAARNKVVYNVLALYYKRIVNKEIRLADIN
                     ENDRILCVGGGPCPFSGILLHEHTKAHVTIIDNDECCVEVSKQLVRKLGYEDSIKIYH
                     GDGSDICPEDYTVIHMAVQVSPLDKVFSCMKQKCKLGSKILVRIPKKHLSHTYNVHDL
                     TVFNAFNSRANHLFRNIDSTALFIVY"
     gene            complement(5265..7046)
                     /locus_tag="PLI11_01085"
     CDS             complement(5265..7046)
                     /locus_tag="PLI11_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPZ51488.1"
                     /translation="MASCHDINEDVNVYNKPNKILLMGNPNVGKSVFFSHLTGINVVS
                     SNYAGTTVTYTEGTIKLDDETYTLIDVPGTYSLEATSEAEAVAVGFMCSNPVAVICVL
                     DATNLERNIKLGLELKKYNVPIVYALNLLDVAKRHGTEVNVNLLSQELGAPVVPTVAV
                     KGEGIDKLIDELKKVIKDPEKAFDCGGCEVCPNCIGESLNEWDRAKEITSKVRTKTNG
                     KPSFLDRLGDNMIKPWPGIPIALIVIVASLGAIVGGGKALRAVALLPLVNNVLVPLFH
                     KLFSAFVPEGILLNVLVGDFGIFKIAFEWIIALILPYVFLFYVVFSFLEDSGVLPRLS
                     VLFDNIMRKMGIQGGSMITILLGYGCAVPAIIGSRTATTKKERLIISTVVCFAVPCIS
                     QTGALISLFANFSPILLILMLLLSFFIMFVVSFILSRTLKGKVDPMVIEIPNLLMPNG
                     KAFGKKLMIRMKGFLVDAEVPMLISIVIAALLKETGLLDFIAVYLEPIVSGWLGLPKD
                     AVIALILGIVRREMAVAPLLAIEGLTALQAFVGGTVALLYLPCLSVFGILTKEFGPKV
                     AITIGLSTVVSAFTVAGLINQVAQLII"
     gene            complement(7052..7318)
                     /locus_tag="PLI11_01090"
     CDS             complement(7052..7318)
                     /locus_tag="PLI11_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPZ51489.1"
                     /translation="MRNRFRRRHGHGQKPDLQIQSIYHAPDNYQYTITKAPGLAILKS
                     LGIVENASVYKVMTHRLGGPVLIKVGSSDIALGKDIAERIMVRR"
     gene            7532..8920
                     /locus_tag="PLI11_01095"
     CDS             7532..8920
                     /locus_tag="PLI11_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51490.1"
                     /translation="MKTKALACMALALMYLTLLFSGINAAVLDIDGEEATINISSKNL
                     KPLKEETISITIPNYSDKLTVTVTDAVGVEYVNEEVLSENGVYTLKFLPRGKAGTHTI
                     TITKTNGASASYTIKMLADTTVETENDYFNKLFEHYKTTITQGQSRHNLQKMNFKVNN
                     IWLRDHIHMMKAAKYFDNDVQSGLDFFIMNQTEEGFYYEIIVNSQDIHTTFVKGDCLK
                     PINASTTLIRLEIEADIEYLMAEAIYQAWKATDDLEWLKEVLPSLEKALNYYLVSDKR
                     FDPGTGLIKRGVSIDTWDWIYGQKGLDRNRMIEEDSPMAIFHGDNTGFYQACIMISEM
                     YRAAGDESNAEKWENTAKDIRKKLMDIALNGNFFSHMVHIRPTVEEVTDALWKVDFEG
                     DKDRLSLSNAYALNRGILTQQEASKIIETYLELRNNPPPTETNPDKKYYAEWVTLYPS
                     YLKLCVAKASRF"
BASE COUNT         2849 a         2022 c         1565 g         2501 t
ORIGIN      
        1 aagcaacagg ttatcattcg tctttacgag aatccttccc tttccttttg tttttatgca
       61 aaaacgtctt acaccatcct tgaaatccag atacttgtac tccgcccagt cctgtatcca
      121 atttccgcca ccgcagttca caagtatttc ctttgaatca aagtatggtg caatatatat
      181 gttgcctttt accctgcatg caattgatgc atcaatctct ttgaatgcat caatgggacc
      241 gccggcaccc atcgatgtca tttcaacctc attgatggtt ccatcttcat tgaaatatat
      301 tttctcaaca cacattcttc ttgaagtaaa gctgttttgc gaggatcgat gatagaaaat
      361 ataccactgt cccttgaatt cttcaataga accgtggttg ttccaggttt gtgggtcaca
      421 gtacatattg tcaataatca cgccgccctt tttgtacgga cccaaaggct tgtcagacat
      481 tgcataagaa agacacgtag ccttccctct tgatatatcc gtatagacga tataatatat
      541 tccgtttctt ttgcgcatgc atgccccttc atggaatcca tgttcctgct ctgttagaat
      601 cgctgtctga agactgtcca tatccaaagt agacatgtca tcattcaact ttgctcccct
      661 taagttgaac tggccccata aatagtatgc ctgtccgtca tcatcaacaa aaactgctgg
      721 gtcaatactg tctccatcag caccttttac tggttttgcg tccccaaaag gcccgtaggg
      781 cttgtcggat actgcaacag cttcaaagtt gtcctgcccg caaacatata gataatattt
      841 tccgtcctta tgaattgcat cgggagcgta caaaggagta cctttgctcc aatatatgcc
      901 aggctcttct ttggtattct tgaatgatat accgtggtat gtccagtttg tcaaagtttc
      961 gtcctccgtt gagaagacat gatactcatg accgcagtat tcctttttgc cggatatgtc
     1021 ggtggagccg tagagataca accttccatc cggcatgaca tgggcttcac catctgcaat
     1081 aaagaacggt cttggcaata tgggattcat tttaattcct ccagtttttc atattcgccc
     1141 ctattatacc aaattgttta ccactttgtt gagcttttcc gtcatcaaaa gcatcaaaac
     1201 tgcaaaaaca gcaagataaa aaggataaaa tccaatattg acataatctg cgataagacc
     1261 aaacaaagga ggcatgaatg tactgcccac gtatgcgcta gccatctgta ttcctatgat
     1321 tgcctgagaa ttttcctttc cgaagttgga aggcgttgag tgtattatgg cagggtaaat
     1381 aggagcacat ccaagacctg caacaacaag acctactaaa gaaagcacac tggagaaagg
     1441 aagagcaatc atcaaaaccc caaacatgca aacaattatc ccacttctaa tcataagcct
     1501 gtctccaacc ctgttggata caaacccgct tataaaccta cctattgtaa ttccaagaaa
     1561 gaacatggac gcaaaggttg cagcagtctc tgcattcaca tttctgcact caacaagata
     1621 actgctggcc catagtcctg ctgttgattc aagagcgatg taactgaaga agcctaccag
     1681 tataagtttt acacccttta tccccacagc ttccttaatg gtgatatggc ggccatttgc
     1741 tgctttgtcc ttcattgcct gaccgttctt tctccaaaga ggaaaacttg taaacagaaa
     1801 cgaggctatg actatctgta tgacagatac agtcagatat cctttcgagt atccgaaacc
     1861 tcttgtaagg cagaaaccca ttatatatgg actaatggtt gctcccacac cccagaaaca
     1921 atgcagatgg ttcatatgtc ttgagttgta gtaaagagcc acatagttgt tcaaggcagc
     1981 gtccacggca cccgccccaa gaccatatgg tattgcaaag atgcagagaa gtatgaatga
     2041 tttcgttatg gaaaacccaa aaagtgcaac tgccgtcatc aggacactga cagaggttac
     2101 aaaataggtt ccaaaccttc ttgtgactct gtcagacata aggcttgaca caatagtgcc
     2161 gcctgcaata atcattgtca caatacctgc atatgacatg ggtacgtcaa gttcatgata
     2221 cataacaggc catgctgacc caagcagtga atcaggcagg ccaaggctaa taaaagcaat
     2281 gtatattata accaacaata gtgaaaacat aagaactcct gattaaaaat aataacctgt
     2341 atattctacc ataaaaacat tatattcgcg gaataggaat tgagtttatt ctttttccgt
     2401 atttgtcaaa aagagatatt acaactttat cggatacact tcctcttaaa gtttcgaaat
     2461 taaagtatga gcatttctca agagtatcta taccaaaatc aagcaccaca ccttcccagt
     2521 ttcccaactc gttcaaacgc cacagataaa tgggctcctt gacggatgga cgaaatgttc
     2581 tcatcatctc ttcataatcc gcatatgcac cccttgttaa gacatagcat tcatccttat
     2641 ggaaaatggc actataaggt ttttcataat acatccattt aggcgtccac aaaaccaaag
     2701 gctggctagt gatgttatca aaatatattt caagtctggc gtaatcatta taataatcat
     2761 attcaccctt cataattaag gacgaatact catccatggc aagaaggtac ctctccgtaa
     2821 tgatattttt aaatgaaggt tctggtgttg tttggtttgt tgactgcgta ggactatttg
     2881 agagaatctc gttgtcatct tcattcttaa ataacaaaaa gacacacaaa aacacaagta
     2941 atacaaaaat tgatattacc catgtatatc tggattcctt tataccagtc tttatttcat
     3001 tttcctcagt ctcttcgccg attacaacat tctcatcaaa ctcgttgtcc atgcaaatcc
     3061 ttctctttct ttatttcctg cgatgcaaca ccatccggtt tctttactac atcatccata
     3121 tcaagacata caccctgcat acatatttta tcacagtttt cttatcaatg cagtattctt
     3181 ttcacacgaa aactgccaac catcaaaaaa gacaacaaaa ttgtctgcaa tacaaataca
     3241 aggacaaaca tatttgaaaa ttcctgtgaa acaaaactat gtatgaaaag cgtgcttgca
     3301 gttatgaaac ctgcagctgt aatcgggagc cctttgaaga acctgtaatc atcctgaagg
     3361 ttgtatcttg caagacgaaa agcacctgcc agaggataca tgacaagtac aaaaaggaca
     3421 taccaggaag gttcctctat acgcgacatg aatattgaaa ttggcgcaac accaaaagtt
     3481 acaaagtcag caaaactatc aagctgcctt cccaattccg atgtggcatt caaatgtctg
     3541 gcaagaaaac catccaagga atcaaaaatt actgccaaat aaatcaaaag acaggcaagt
     3601 cttgtgcctg taacagaatt actgcgcatc atagtaagta tgacaagtac acccactgcc
     3661 atattcgaaa atgtaagcat gttaggaaca tgtttggttt tttggaacat tcttttttct
     3721 ttctccaatt ttccatcctc caatatacag aagactcagc aatataacga accagaaggt
     3781 gataaacctg aaaataagcg taattgccaa agcttcgttt ttctcaattt gcattaatac
     3841 aagcaggctt gtcattgcag tttcaaaccc acccaatcct cccggcatca gcggtatcat
     3901 tcctaccatg taggaaatga aagtaatctc ggttaggaat acccaattat attccgctgt
     3961 aaataaattt acaagcaata tcattttcaa agggtaaata atccatattg caaaggaaag
     4021 aagaaactgt ttatacatct ccccctttat gctttttaaa accttgatat tcctcaaaag
     4081 aaccatcata tatttatgca gtgcaatgct tgcttttctc tttggttttc ttttcttaac
     4141 cttgttttcc agtatattgg ttccaacaaa cataattaca agaagtccaa ggaatgttaa
     4201 caagaagaga aacacgacaa ctctgacaaa accttgaaat attgccactt tgcctgacaa
     4261 atgggcaaat gcaaaaaggt ttatcacaaa gaaactgaaa aagctgacca ttttctgaat
     4321 tgtaaccagt gttgcggctt cctgataaga atagttcatc tcgctcttca aaagataagc
     4381 ccttgtaact tctcctccaa gctttactcc aggagtaata ctctccatta tcatgcctct
     4441 tgcattaata taaagcatct taaagaagtc gttttcctta ttcatcacct ttccaatcct
     4501 gcaccactgg taattaacaa gaagctgact aacaacctga attaaaagaa gaccaataat
     4561 tcctaaaaca ggtactttcc taatactatc tacaaaactt tttaaatcaa atgtcagaat
     4621 caatatgaca aataaaaaaa ggagtacgac ataccctgtt cttcttacta ccctcatcca
     4681 aatactctcc ttaatagacg atgaaaagcg ccgtactgtc aatatttcta aaaagatgat
     4741 ttgccctgct gttaaaggca ttgaacaccg tcaggtcatg cacattgtaa gtatgggata
     4801 aatgtttttt aggtatgcgt accagtatct ttgagccaag tttgcatttt tgtttcatgc
     4861 agctgaacac cttgtcaaga ggacttacct gcaccgccat gtgaataaca gtgtagtctt
     4921 caggacagat gtcgctacca tcgccatgat aaatcttaat ggaatcctca tatcccagtt
     4981 tcctgacaag ctgtttcgaa acttctacac agcactcgtc attatctatg attgttacat
     5041 gagcttttgt atgctcatgc aacaatattc cgctaaaagg acaaggtcct cctccaacac
     5101 acaaaatcct gtcattctca tttatatcag caagccttat ctccttgttc acaatccttt
     5161 tgtaatacaa ggcaaggaca ttgtatacaa ctttgtttct tgcagccagg cattcaagtt
     5221 tctttgtggt tctttgtatc aagcctgcca taacaatccc tcatttatat aatcagttgt
     5281 gctacttgat tgataagtcc ggcgactgta aatgcactta caactgtaga cagccctatg
     5341 gttatagcca cctttggacc aaactctttt gtcaggattc caaacaccga caagcagggc
     5401 aggtacaata gcgcaaccgt tccgcctaca aaggcttgaa gtgctgtcaa accttcaata
     5461 gccagcagtg gtgcaacagc catttccctg cggactatac caaggatcag tgcaataaca
     5521 gcatcctttg gaagaccaag ccatccactt acaattggct ctagatatac cgctataaaa
     5581 tcaagaagtc ctgtttcctt aagcagcgct gcaataacta tggatatgag catggggact
     5641 tccgcatcta caaggaagcc tttcattctt atcatgagtt tctttccaaa agccttgccg
     5701 tttggcataa gcaggttcgg tatctcaatt accatcggat ctacttttcc tttaagcgtg
     5761 cggcttaaaa taaaggatac tacaaacata ataaaaaacg acaaaaggag catgagtata
     5821 agcagtatgg gcgaaaagtt cgcaaacaga ctgattagcg ctccggtttg ggagatgcat
     5881 ggtactgcaa aacacacaac agtggaaatt ataaggcgtt ccttcttcgt tgtggcggtt
     5941 cttgaaccga taattgcagg caccgcacac ccatatccaa gaaggattgt aatcatgctg
     6001 ccaccctgta tgcccatctt cctcataata ttgtcaaaga gaacactgag tcttggcaat
     6061 acacccgaat cctcaagaaa agaaaacacc acataaaaca aaaagacata aggaagaatc
     6121 agggcaataa tccactcaaa ggcaatcttg aatattccaa agtctccaac cagcacattt
     6181 agaagtattc cttcaggcac aaaagccgag aagagtttgt gaaacaaggg aacaagcaca
     6241 ttgtttacca aaggaagcaa agctactgcc ctaagagctt ttcctccacc aacaatggct
     6301 cccaaagacg caacaattac aatcagcgca atgggtatac ccggccaggg ctttatcatg
     6361 ttgtctccca gtctgtcaag aaagcttggt ttgccgtttg ttttcgtcct aactttcgaa
     6421 gttatttcct ttgccctgtc ccattcattc agactttcac ctatgcaatt tggacaaact
     6481 tcacaacctc cgcaatcaaa agccttttcg ggatccttta taaccttctt caactcatct
     6541 ataagtttgt caataccttc acccttaaca gcaaccgtag ggactacggg agccccaagc
     6601 tcctgggaca aaagattcac attaacttca gtaccatggc gttttgccac atccaaaagg
     6661 tttagcgcat atactatggg tacattgtat tttttaagct caagacccag tttaatattc
     6721 ctctcaaggt tcgtagcatc aagaacacaa atgacagcaa caggattgct gcacataaaa
     6781 cctacagcta cagcttccgc ttcgcttgtt gcttccaatg aatatgttcc tggtacgtct
     6841 atcagggtat aagtttcatc atcaagtttg attgttcctt cagtatatgt aaccgttgtg
     6901 ccggcatagt ttgaacttac tacattgatt ccagtgagat gtgaaaagaa tacgctcttt
     6961 cctacattag ggttgcccat taaaagaatt ttattgggct tgttatacac atttacatcc
     7021 tcattaatat catgacaact tgccaagtaa actacctcct taccattatt ctctctgcta
     7081 tgtccttgcc aagggcaata tcgctggagc ccaccttgat aagtaccggg cctccaagtc
     7141 tgtgagtcat aactttgtaa accgatgcat tctcaactat acccaagctt tttagaatag
     7201 caagcccggg tgccttcgta atggtatatt ggtaattatc cggggcatgg taaatacttt
     7261 gaatctgcaa atccggcttc tgtccatgtc catgtctgcg cctaaacctg tttctcattc
     7321 gtcatcttcc cttcacatta gcacattaaa aaagttaggt tcttctaaca tgtatattat
     7381 tagcacagag cgtataagat gtcaatatga aaaagggcat aattcacaat atttttagat
     7441 ttgtctaaca aatcttctca aaagaacaca atagtatttt tcacaaaaat ataatataat
     7501 tgacatacat tacaggaagg aaggtgaaac aatgaaaact aaagcattgg catgcatggc
     7561 tcttgcatta atgtacctga ccctactttt ttcaggtatc aatgctgcag ttttagatat
     7621 tgatggtgaa gaggctacca ttaacatatc gagtaaaaat ctgaaaccgc ttaaagaaga
     7681 aacaatctca ataacgatcc caaactattc cgacaaactt acagtcaccg ttaccgacgc
     7741 agtaggcgta gagtacgtaa acgaagaagt tctgtcagag aatggagttt acacattgaa
     7801 atttttgcca agaggcaagg caggaacaca tactatcact attacaaaga caaatggagc
     7861 atccgccagc tatactataa aaatgctggc tgacacaact gtcgagacag aaaatgatta
     7921 cttcaacaaa ttgtttgaac actataaaac gacaataaca caaggccagt ccagacataa
     7981 tttgcagaaa atgaatttca aagtaaacaa catatggtta agagaccaca ttcatatgat
     8041 gaaagctgcc aagtattttg acaatgatgt acaatctgga cttgacttct tcatcatgaa
     8101 ccagaccgag gaaggcttct actacgagat tattgtaaac agtcaggata tacacaccac
     8161 ttttgtaaaa ggtgactgcc tcaaaccaat aaatgcatca accacgttga taaggcttga
     8221 gattgaagcg gatattgaat acttgatggc cgaagccatt taccaggcat ggaaagcaac
     8281 cgatgatctt gaatggctga aagaagtttt gccaagcctt gaaaaagcac ttaattacta
     8341 tcttgtaagc gacaagcgat ttgacccagg cacaggcctt atcaaacgag gagtatccat
     8401 tgatacatgg gactggattt acggccaaaa ggggctggac cgcaacagaa tgatagaaga
     8461 agactcacca atggcaatat tccacggaga caataccgga ttctatcagg catgcatcat
     8521 gatttcggaa atgtaccgtg cagcaggcga tgaatcaaat gcagaaaagt gggaaaacac
     8581 cgcaaaagac atacgcaaaa agctcatgga cattgcattg aacggaaact tcttttccca
     8641 tatggtacat atacggccga cagtggaaga ggtaaccgat gctttgtgga aagtagactt
     8701 tgaaggagat aaagacaggc tcagcctaag caatgcttat gcactaaaca gaggaatttt
     8761 gacacagcag gaagcaagca agataattga gacatacctt gaactaagga acaaccctcc
     8821 tccaacggaa acaaatccag acaagaagta ttatgctgaa tgggttactc tctacccttc
     8881 ctacctgaaa ctctgcgtgg ctaaagccag caggttctaa cgtactttcc aacttct
//
LOCUS       DAONVY010000020         8713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6182_length_8713_cov_4.033033, whole genome shotgun sequence.
ACCESSION   DAONVY010000020 DAONVY010000000
VERSION     DAONVY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6182_length_8713_cov_4.033033"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /gene="cysS"
                     /locus_tag="PLI11_01100"
     CDS             <1..1213
                     /gene="cysS"
                     /locus_tag="PLI11_01100"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPZ51491.1"
                     /translation="MNITDVGHLVSDADEGEDKMAKAAKAQQKTPEEIAEYYTNVFFE
                     DIKSLNIETPEIVVKATDHIMSMIEFVVSLVEKGYAYETSDGIYFDIEKFPEYGKLSR
                     INLEDQLAGARVSVNDEKRHPADFALWKKAPKEHIMQWNSPWGMGYPGWHIECSAMAR
                     DYLGEEFDIHTGGVDHIPIHHENEIAQSEALTGKQTVKYWMHAEFLLVDGGKMSKSLG
                     NTYTIKDLKHRGFEPLAYRYFCLNGHYRNKLNFTFDALTAASKALDRFYEALSQHKEG
                     KDNVSDELIEDFEKEFLDAVNDDLNIPQALGVVWKALRNTEKSRKIYDFVIKTDNVLG
                     LDMENQVAKREEAKRNAESKVSDEVLELIRIRAEAKKNKDYAKADAIRDQLKEMGIKI
                     IDTPEGTKIVD"
     gene            1219..1788
                     /gene="sigH"
                     /locus_tag="PLI11_01105"
     CDS             1219..1788
                     /gene="sigH"
                     /locus_tag="PLI11_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HPZ51492.1"
                     /translation="MNDEKLVMLAQQGSDQHMELLLVKYKGLVRTVTRAYFLTGADGE
                     DLLQEGMIGLYKAILSFAPGKNVNFGKYAKICIKRQVISAVKSANRLKHLPLNSYVSI
                     YKENEDDCEMDCLTTSELSNPEEIAISKESAEIFKKKIDTLLSEFEKKVLVMFLNGDS
                     YESIANTLNKETKAIDNALQRVKRKLKNA"
     gene            complement(1785..2753)
                     /locus_tag="PLI11_01110"
     CDS             complement(1785..2753)
                     /locus_tag="PLI11_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51493.1"
                     /translation="MKLQKITSGIAIFIAAIIYVIFAPQTVYSDTVGFDTAKLLSELG
                     IFYIDKDMSDKDLSDFLKEESTKAIAYNLAYGLTGAVRDPYTGLIDTSFSLRFFKSEN
                     LEVYTNMLNTMEYITSTELSPADCYRVFLDVLGYESMQEKGDEEVLLAAQNAGFGYFQ
                     TVENKETITNGELVNITFEALYLCGVNTKIPTIHLLSQINPNFKSKAVKLGMIDLFPD
                     EIPLFTEGPYKVQSFSELIGTDVTTNIKKHEWSALYLNVNMEGYQNYKSKLVESDWIF
                     EVEFYKESNDTEDLCVLYYKEIDGIEHYVTLVFNEESKTVELWYAY"
     gene            3102..3692
                     /locus_tag="PLI11_01115"
     CDS             3102..3692
                     /locus_tag="PLI11_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51494.1"
                     /translation="MLFSAADMKFLVNDIYYFILYFLTSACYITSLLLIIAFCILLLN
                     MLYRKCCLIASWGLMSATMIMRNVTYCMKYNQRPFCSDNLVISMLAFCLLLSLFVVFQ
                     DFENVFFAHILGWISITLSTIEYITSGIMLTPVAGYFILLSFTARDISSMMNVFYTEI
                     LESKLKTLRKKYDWRKISEDEYKERYDRLIDKYQIG"
     gene            3760..4725
                     /locus_tag="PLI11_01120"
     CDS             3760..4725
                     /locus_tag="PLI11_01120"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPZ51495.1"
                     /translation="MRITVVGCGRWGSCIAWYCQAIKKHDVLLYGLKGSRDYLRFVET
                     RRNDYITLPDDIELTDDLEYAMSYSKVIVVSIGAQQFRSFLKDLVGRVELNDKIFILC
                     MKGLESETGKRLTQIFHEEVGPSNKVAIWVGPGHVQDFYNGRPSCMIIGSDDIEVTKF
                     LVSELNSTLIRFYYGQDLIGCEVGAAAKNVMGIAAGMLDGFDWPGLKGALMARGAREI
                     SRLVRAMGGNELTVYGLSHLGDYQATLFSEYSHNRLFGENYIKGVPMNKLAEGVDTAK
                     ALVRLSELYNVELPISKAVYSVLFEGKDAKEMLDSMFIRETKFEF"
     gene            4728..5201
                     /locus_tag="PLI11_01125"
     CDS             4728..5201
                     /locus_tag="PLI11_01125"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPZ51496.1"
                     /translation="MKRKSLIFFIIFLIFVLSSCGQGGVNLTPVPTDTLLPTHTLTPS
                     PSPTLEPTEEPEVEQTIRISFVGDCMFGMQNEFDLPDLFPQVYERSNSVTYPFDYVLD
                     YFKSDDITVINYEGTLTNHTKQADKPWRFRGKPEYANILKESSVEIALLANNHFL"
     gene            5179..5880
                     /locus_tag="PLI11_01130"
     CDS             5179..5880
                     /locus_tag="PLI11_01130"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPZ51497.1"
                     /translation="MPTIISYDYLEEGYIETRNAFKSHGVEPLGHNEVYKVSVKDVEI
                     VFIAANFILVEDTNVMAERYEDVLPLIRENKKEDNIVIVNLHWGKELEKVPPKEQRDV
                     AHLYIDEGADLIVGHHPHLLQGIENYKGKYIVYSLGNFAFGGSRSATNPDTIIYVHEW
                     TVKGGKILGDDSFAVPCYITSTDELNFKGVLANNFRPIPLEDERKQACINLILERSKP
                     LDHGIEAVSVLELSL"
     gene            complement(5869..7407)
                     /locus_tag="PLI11_01135"
     CDS             complement(5869..7407)
                     /locus_tag="PLI11_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPZ51498.1"
                     /translation="MKRKIVILILLVIAILTTSCATSVKLTEEDKEFITRYYTEYESD
                     LFDAENIVLTFAAMSDTHIGGKNQPEKLAKAIKLLNKRAKYRLDALLFAGDLTDVTGY
                     KVQEDEISIFKKVIEENKDKNTSILYILGNHDVNDEVSMSPSFYRIFGEAFYNANVLE
                     KEEILSGKHHWIVNGYHFLGADFDWSADDYVGKNLEWIDTKLKEITEKEPNKPVFVTV
                     HAPRTNVDRVLSKYPQVILLTGHTHLPLNSDRAIKQTTYTHLHCGGMYYYRQYTVDNK
                     LMDDLGEIHNFMQGYLIDVDKNHNVRVIRMDFYHEKAYEPVWIIPAPIEDKSHLLYTE
                     ELKDKSKRPYFEDTEIDLSDSMTYTFVGRFKAAKTESTNPIELYEIKLWQTDKVDTKY
                     LSTYYAITEKEDFPDEYEFRFIRVDADIHAVSISAYDCHGSSSNNILYNKGLYFVDIR
                     SNVPGNANITAYRPGENVRIKITPPSGYRIESISAEGVELDDSMSFVMPENNVIVNVT
                     YSKR"
     gene            complement(7538..8116)
                     /locus_tag="PLI11_01140"
     CDS             complement(7538..8116)
                     /locus_tag="PLI11_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPZ51499.1"
                     /translation="MFNLIYKELRLSAHPNLFIFTLLGILVIVPAYPYEMVFIFGCLG
                     PYITTVYGRETNDIYYTALLPVRKTDIVKAKCLVFVLAQMTQILISIPFAFLRVVVLP
                     AGNPAGIEANVAYYGFGFIIYAVFNMIFLVTFFKTAYKAGKAVLQAIIPASLIMILME
                     VLVHFPQFKWLDSTRANDLPKQFPILLFGIVF"
     gene            complement(8271..8573)
                     /locus_tag="PLI11_01145"
     CDS             complement(8271..8573)
                     /locus_tag="PLI11_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ51500.1"
                     /translation="MTAVTKRFYKNWDEDAYQNYLKVFSLDPNKRVKELSSGMKVKYM
                     LALALSHNARLLILDEPTSGLDPVARDDLLTLFKLLVEKKNITILYSTHIITDLEK"
     gene            complement(8495..>8713)
                     /locus_tag="PLI11_01150"
     CDS             complement(8495..>8713)
                     /locus_tag="PLI11_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51501.1"
                     /translation="MLNLVKRDGGSVEMFGMDFFKHEKEIKQHLGVVLGGIDFYNHKS
                     FQTLPLSQNAFIKTGTKTHIKIILRSSH"
BASE COUNT         2626 a         1528 c         1880 g         2679 t
ORIGIN      
        1 aatgaatatt accgatgtag gccatcttgt gtccgatgcg gatgaaggcg aggacaaaat
       61 ggcgaaggct gcaaaagcac agcaaaaaac accagaagaa attgccgagt attacactaa
      121 tgtatttttt gaagatatta aaagcttgaa tattgaaact cctgagattg ttgtaaaagc
      181 cactgaccac ataatgagta tgattgagtt tgtcgtatcc ttggtggaaa agggttatgc
      241 ttatgaaacc agtgatggaa tatattttga tattgaaaag tttccagagt acggcaagct
      301 ttctagaata aacctggagg accagctggc aggagcaagg gtttccgtaa acgacgagaa
      361 gagacacccg gctgattttg cattgtggaa aaaggctcca aaagagcaca ttatgcagtg
      421 gaacagtcct tggggaatgg gttatccggg atggcatatc gaatgctctg ccatggcaag
      481 agattatctt ggtgaagagt ttgacatcca tactggcgga gttgaccaca tacccataca
      541 tcacgagaat gagattgccc agtctgaagc tcttacagga aagcagactg taaagtactg
      601 gatgcatgca gagtttcttt tggttgacgg cggcaaaatg tcaaaaagtc ttggcaacac
      661 atacacaata aaggacctta aacatagggg atttgagcct ttggcctaca gatatttctg
      721 cttgaacggt cactacagga acaagctgaa ctttactttt gatgcattga ctgcagcgtc
      781 caaggctttg gataggttct atgaggcatt aagccagcac aaggaaggca aggataatgt
      841 atctgatgag ttgattgaag actttgaaaa ggagtttctt gatgcagtca atgatgattt
      901 gaatatccct caggctttgg gtgttgtgtg gaaggctcta agaaataccg agaagagcag
      961 gaagatatat gattttgtaa tcaagactga caatgtattg ggtcttgata tggaaaatca
     1021 ggttgccaag agggaagagg caaaaagaaa tgcagaaagc aaggtcagtg atgaggtcct
     1081 tgagcttata cgcataaggg cggaggccaa gaaaaacaag gattatgcca aagctgatgc
     1141 tatcagggat cagctgaaag agatgggaat caagataatt gatactcctg aaggaacgaa
     1201 aattgtggac tgatatttat gaatgacgaa aaattggtaa tgcttgcaca acagggcagt
     1261 gaccagcata tggaattatt gctggtaaag tacaaaggtc ttgttagaac agtaactaga
     1321 gcctattttc ttaccggtgc tgatggcgag gatttgcttc aggaaggaat gataggctta
     1381 tataaggcta ttctgtcgtt tgctcctggc aagaatgtca atttcggaaa atatgcgaaa
     1441 atatgcataa agagacaggt gataagtgct gttaaatctg ccaacagact taaacacctg
     1501 cctcttaatt cttatgtatc catatacaag gaaaatgagg acgattgtga gatggattgt
     1561 ctcaccacat cagaattgtc aaaccctgaa gagattgcca ttagcaaaga atctgctgaa
     1621 attttcaaga aaaagataga tacgcttctt agtgagtttg aaaaaaaagt ccttgttatg
     1681 tttcttaatg gagattcgta tgaaagtatt gcaaatacgt tgaacaagga aaccaaggct
     1741 attgacaatg ctctgcaaag agtaaaaaga aaactcaaga atgcttaata tgcataccag
     1801 agttccacgg tttttgattc ttcattgaat acaagagtta catagtgttc tataccatca
     1861 atttctttat agtataatac gcagaggtct tcggtatcgt tcgattcttt atagaattca
     1921 acttcaaaaa tccaatctga ttctaccagt ttgcttttgt agttttggta gccttccatg
     1981 tttacattca aatagagggc agaccattca tgttttttta tgttggtggt gacatccgta
     2041 cctataagtt ccgaaaagga ttggactttg tatggtcctt cagtaaacaa gggaatttca
     2101 tcaggaaaca aatctatcat gccaagcttt actgctttgg atttaaaatt cggattaatc
     2161 tgtgacagca aatgtattgt tgggatcttt gtattcacac cgcagagata caatgcttca
     2221 aaggtaatat ttacaagctc gccgttggtt atcgtttctt tgttttcgac agtctgaaaa
     2281 tacccaaagc ctgcattttg tgcagctaga agaacttctt catcgccttt ttcctgcatt
     2341 gattcataac ccagtacatc aagaaatacc ctgtaacagt ctgcaggact taattcagtg
     2401 gatgtaatgt attccattgt attaagcatg ttggtataaa cttccaggtt ctcgcttttg
     2461 aaaaagcgca gcgaaaaact tgtgtcaatt aatccagtat agggatctct gacagcaccg
     2521 gtcagaccat aagccagatt gtatgcaatt gcctttgtgg attcttcttt gaggaagtct
     2581 gaaagatcct tgtcgctcat gtctttgtct atgtaaaaga tgccaagctc gctcaataat
     2641 ttggcggtat cgaatcccac cgtatccgaa taaaccgtct gtggagcgaa tattacataa
     2701 attattgctg cgataaaaat tgcaattcct gatgttatct tttgtaattt catttatacc
     2761 ctccaggatc ctttagtagg catcggttat attttaacag caatacagcc atattgcaag
     2821 acatacaatt ttctaatact tgactatttt tcttttttat atataatata atgtaactaa
     2881 aagaaacggc atttatggag gtacaaatgt tgaacaaaat attggattga cattaggtgt
     2941 ttttatccac tgaagattta ggaattttgc atttgatgct tagatgaaag tgcaaacagt
     3001 cgaatcacaa acggaggatg aatgtgacaa ccaatttgaa atacaaacta ttcctacaga
     3061 tttttattta tctccttgct attttgtgga gtttgcccac attgcttttc agtgcggcgg
     3121 atatgaaatt tcttgttaac gacatctatt atttcattct atatttttta acaagcgcat
     3181 gttacattac aagtctttta ctcattattg cattttgcat actgctattg aacatgcttt
     3241 acagaaagtg ctgtcttata gcttcgtggg ggcttatgag tgccaccatg attatgcgta
     3301 atgtgacata ctgtatgaag tacaatcaaa gacctttttg ctctgacaac cttgttatct
     3361 caatgctggc tttttgcttg ttgttgtctt tattcgtagt atttcaggat tttgaaaacg
     3421 tattttttgc acatatactg ggatggatat ccataacatt atccaccatt gaatatatta
     3481 caagtggaat aatgctgact cctgttgccg ggtatttcat actgctatcc tttacagcaa
     3541 gagatatctc aagtatgatg aatgtctttt atactgaaat tcttgaaagc aagttaaaga
     3601 ctcttagaaa gaaatatgac tggcgaaaaa tatctgaaga tgagtacaaa gaaagatatg
     3661 acagactgat tgacaaatat cagattggtt agttgcattt ctgcatgaaa atggtatact
     3721 aaatagtgta gaaaaaagaa taaaggattg attttcagaa tgagaataac tgttgttggt
     3781 tgtggcaggt ggggcagctg cattgcttgg tactgtcaag cgataaaaaa acacgatgta
     3841 ctgctctacg gtcttaaagg ttcacgtgat tatttaaggt ttgtggaaac aagaagaaac
     3901 gattacatta ctcttcctga tgatatagaa ttgacagatg atttggaata tgcaatgagt
     3961 tattcaaaag tgatagttgt gtcgattggc gcacaacagt tcagatcctt tttgaaggac
     4021 ttggttggcc gtgttgagct taacgacaaa atcttcatat tatgtatgaa gggtcttgaa
     4081 agtgaaacag gaaaaaggct gacacagata ttccatgagg aggttggccc tagcaacaag
     4141 gttgccatat gggtaggtcc tggtcatgtt caggattttt acaacggaag acccagctgc
     4201 atgatcattg gatctgatga cattgaggtt acaaagtttt tggtatcaga gcttaacagc
     4261 actcttatac gattctatta cggtcaggat ctaatagggt gtgaggtagg tgctgctgcc
     4321 aaaaacgtca tgggtattgc tgcaggtatg cttgatggtt ttgactggcc aggattgaaa
     4381 ggggctctta tggcaagagg agcaagagag atttcaagac tggtaagggc aatgggaggc
     4441 aatgaactta cagtttatgg tctttcgcat cttggagatt accaggccac tctgttttca
     4501 gaatacagtc acaacagatt gtttggtgaa aactatataa aaggtgttcc gatgaacaag
     4561 ctggcagaag gtgttgatac agcaaaggca ttggtaaggc tttcggagtt gtacaatgtg
     4621 gaactgccaa tatcaaaggc ggtttacagt gttctttttg aaggcaagga tgcaaaagaa
     4681 atgctggaca gcatgtttat aagagaaacg aagtttgagt tctgatcatg aaaagaaaat
     4741 cattaatttt ctttattata ttcctgatat ttgtactgtc atcctgtggg caaggaggag
     4801 taaacttaac tcctgtgccc acagatactt tgctgcctac acatacatta acaccttcgc
     4861 catctcccac tcttgagccg acagaagagc ctgaagtcga acagacaatc agaataagtt
     4921 ttgtcgggga ctgcatgttt ggaatgcaaa atgaatttga cttgcctgat ttgtttcctc
     4981 aggtctatga aagatccaat agcgtgacat atccttttga ttatgttctt gattatttca
     5041 agagcgacga tataacagta atcaattatg agggaaccct tacaaatcat acgaagcagg
     5101 cagacaagcc ctggcgtttt agagggaagc ctgagtatgc caacatatta aaggaatcaa
     5161 gcgtggagat tgcactcctt gccaacaatc atttcttatg attacctgga ggaagggtat
     5221 atcgaaacac gtaatgcgtt taaaagtcat ggtgtggaac ctttagggca taacgaggtg
     5281 tacaaggtta gtgtaaaaga cgttgagatt gtatttattg ctgcaaactt tattctggtt
     5341 gaggacacca atgtaatggc agaaagatac gaggatgttc ttccattgat aagggagaac
     5401 aagaaagagg acaatattgt aatagtcaac ctgcactggg gcaaggagct tgaaaaagtg
     5461 ccgccaaaag aacaaaggga tgtagcccat ttgtatatag atgaaggggc ggatctgata
     5521 gtaggacacc accctcatct tcttcaagga attgagaact acaaggggaa atatatagta
     5581 tacagtcttg gaaactttgc ttttggcggg tctagaagcg caacaaaccc cgataccatt
     5641 atttatgtac atgaatggac ggtaaagggt ggtaaaattc ttggcgatga cagttttgcc
     5701 gttccatgtt acataacatc aacagatgaa cttaatttca aaggggtttt ggcaaacaat
     5761 ttcagaccca ttcctttgga agatgaaaga aaacaagcat gcataaattt aatactcgag
     5821 aggagcaagc ctctcgatca tggaattgaa gcagtaagcg ttttggaatt atctctttga
     5881 ataagtcaca ttaacaatca cattgttttc cggcataacg aaggacattg aatcatcaag
     5941 ctccacacct tctgcagaaa tactttcaat acggtaccca gaaggaggag ttatttttat
     6001 ccttacattt tctcctggcc tgtatgcagt tatatttgca ttgccaggta cattggatcg
     6061 aatatcaaca aagtacaggc ctttgttgta aaggatgttg tttgacgagc tgccgtggca
     6121 gtcgtaagcg gagatgctta cagcatggat gtctgcatca acccggataa acctgaattc
     6181 atactcatca ggaaaatcct ccttctcggt aatggcgtaa taagtggaga gatatttggt
     6241 atcaaccttg tcagtctgcc atagtttaat ttcgtaaagc tcaattggat ttgtgctttc
     6301 tgtctttgca gccttgaatc tgccaacaaa tgtatatgtc atggagtcgc tgagatcaat
     6361 ttccgtatct tcgaagtatg gtctctttga tttgtcctta agttcttctg tatacagcag
     6421 atgggatttg tcttctattg gtgctggaat aatccacaca ggctcatatg ctttttcgtg
     6481 ataaaaatcc attcgtataa ctctgacatt atggttcttg tcgacatcaa taaggtagcc
     6541 ttgcatgaaa ttatgtattt ctccgagatc atccataagt ttgttgtcaa ctgtatattg
     6601 gcggtaataa tacatgccgc cacagtgcag gtgtgtgtaa gttgtctgct ttatcgccct
     6661 gtctgagttg agggggagat gggtatgtcc tgtcaaaaga attacctgag gatatttgga
     6721 aagtactctg tccacattgg tcctcggagc gtgaactgtg acgaaaacag gtttgttggg
     6781 ctctttttct gttatttcct taagttttgt gtcaatccat tcaagattct tgccaacata
     6841 gtcatcagca ctccagtcaa aatcagcccc gagaaaatga tatccattta caatccagtg
     6901 atgtttgcct gaaaggattt cttctttctc aagaacattt gcattataaa aagcttcacc
     6961 aaagatcctg tagaagctgg ggctcataga tacttcgtca ttaacatcat ggttgccaag
     7021 gatgtacaat attgatgtgt ttttgtcctt gttttcttca attacttttt taaatatgct
     7081 tatttcatcc tcttgtacct tgtatccggt aacgtctgtc agatctcctg caaacaatag
     7141 tgcgtccagc ctatattttg ctcttttgtt aagcaatttt atggcctttg caagtttctc
     7201 gggctggttc ttgcccccta tatgggtatc actcatggcg gcaaatgtaa ggactatgtt
     7261 ttcagcatca aaaagatcgc tttcgtactc ggtgtagtat ctggttataa attccttgtc
     7321 ttcctccgtt agcttgacac ttgttgcaca tgaggtagtt agtattgcaa taacgagcag
     7381 aataagaata actatttttc ttttcatata tacacctctg gttttcgcct ggtttgaaac
     7441 caatattaca ttagaaagtg cataccgtca accactaaag atcaactttt gaaaacctgt
     7501 ttgcagatac cttgtaagtc agcgacatga ttatggttta gaacacaatg ccaaatagaa
     7561 gtatggggaa ttgttttggc aaatcgtttg ctcttgtgct gtcaagccac ttgaattgtg
     7621 gaaagtggac aagtacttcc ataagaatca tgatcaagga tgctggtatt attgcctgca
     7681 ggacggcttt gcctgccttg tatgcggttt taaagaaagt aacgaggaat atcatgttga
     7741 agactgcata aattatgaaa ccaaaaccgt aatatgccac attggcttca atacctgcgg
     7801 gattgccagc aggcagcaca acaactcgca gaaaggcaaa gggaatggat attagtattt
     7861 gggtcatttg agcaagaaca aatacaaggc attttgcctt tacaatatct gtttttctta
     7921 caggaagcag ggcggtgtag taaatatcat tggtttctct gccatatacc gtagttatat
     7981 atggaccgag gcatccgaag atgaatacca tttcataggg ataagcaggt acaataacca
     8041 gtatgccaag cagtgtaaat atgaaaaggt ttgggtgggc ggaaagtctt aattctttat
     8101 atatcagatt aaacattgta ttctctcctt tccattctta tcattatttc ttccaaggaa
     8161 ggatgtgctt tctcatttgg ttttctcaaa tactgaaagg aatctatgaa ttctttcttt
     8221 tcagcgcttt taacaagttt cccgtctttt atataggtaa tacagtctgc tcatttttca
     8281 aggtctgtga ttatatgggt tgagtaaaga atagtaatat tctttttctc cacaagcaat
     8341 ttaaataggg taagcaggtc atcccttgct acagggtcga gtccgctcgt aggttcatca
     8401 agaatcagca gtcttgcatt atgggaaagg gcaagagcca gcatgtattt taccttcatg
     8461 ccacttgaca gttctttgac tcttttgtta ggatctaatg agaagacctt aagataattt
     8521 tgatatgcgt cttcgtccca gtttttataa aagcgttttg tgacagcggt aatgtctgaa
     8581 agcttttatg gttgtaaaag tcaatgcctc caagcaccac acccagatgc tgttttattt
     8641 ctttttcatg cttgaaaaaa tccatgccaa acatctcgac tgaaccgcca tcacgtttca
     8701 ccaggtttag cat
//
LOCUS       DAONVY010000021         8690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6212_length_8690_cov_3.186103, whole genome shotgun sequence.
ACCESSION   DAONVY010000021 DAONVY010000000
VERSION     DAONVY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_8690_cov_3.186103"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="hemW"
                     /locus_tag="PLI11_01155"
     CDS             complement(<1..981)
                     /gene="hemW"
                     /locus_tag="PLI11_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPZ51502.1"
                     /translation="MHAEDFGIYVHIPFCERKCIYCDFLSFGINTIGNESVEKYFDTL
                     YLEMNKKKESVNKTITSIFIGGGTPSIVEPKLIHSLIEKLRKNFVIAKDAEITIEANP
                     GTVKKDDFLMYKDAGINRISFGIQSLNDDLAKFLGRIHTKEDSINAIRNAQKAGFENI
                     NADVLVGVPNQSVDVFMSTLIELANLNVPHISAYSLILEEETSLYDMIDKGIVKLPKD
                     ELDRQMYHKCLEYLMSKEYIHYEISNFALNGYECRHNLLYWHCNEYLGLGLGASSYIN
                     SIRFRNTSNFRDYLSLDFNVFNEEVLSDTDKMNEFMLLGFRLTKGPDPKMF"
     gene            complement(983..2788)
                     /gene="lepA"
                     /locus_tag="PLI11_01160"
     CDS             complement(983..2788)
                     /gene="lepA"
                     /locus_tag="PLI11_01160"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPZ51503.1"
                     /translation="MNNQDKIRNFCIIAHIDHGKSTLADRILEQTGELSEREMEEQVL
                     DNMDLERERGITIKARSVRLNYLADDGVEYIYNLIDTPGHVDFTYEVSRTLAACEGAI
                     LVVDAAQGIEAQTLANVYMAMDHNLEIIPVINKIDLPAAQPEVVKKEIEEVIGIPASD
                     APLISAKAGIGIKDVLEEIATRIPSPSGDKEAPLQALIFDSVYDTYRGVIIYVKVVNG
                     RLKKGDTIKLMSTEKTFIITELGWFKPGTFYPCDSLETGEVGYIVANIKNIQDANVGD
                     TVTLVSNPAKNPLPGYKKAKPMVFCGVYPVDGSDFEGLRDALAKLQLNDASLAFEPET
                     SKALGFGFRCGFLGLLHMEIILERIQREFDIDIITTTPSVIYRIYKTNGEVLELDNPT
                     NMPSVNMIDHIEEPMVKASILTPAEFVGNIMELTIDRRGTFLNMDYLERDRVLLTYEI
                     PLNEVIYDYFDALKSRTKGYASLDYEMTGYKVSKLVRLDIYVNGEVVDALSFIVHEEK
                     AYQRGRKVAEKLKEAIPRHQFEVPIQAYLGGKIIARETVKAYRKDVTSKCYGGDISRK
                     KKLLEKQKEGKKRMRQFGSVEIPQEAFLTVLKLDD"
     gene            complement(2809..4068)
                     /locus_tag="PLI11_01165"
     CDS             complement(2809..4068)
                     /locus_tag="PLI11_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HPZ51504.1"
                     /translation="MNRRIYDSSHLYLIENNRLQKKKRIKKYMLLFIIIEILVISILV
                     VCALVTVDNKGKIALKELLENMDEAKTHNYNGRYLSNRIYGVASPLVKRIHTHEKLVD
                     LYLNGTSPSVSKPPKPNNTPMPVEENIKYYMFSLYDNSYKYSVFYDTEISVETGIKDD
                     IEKELVAGYISELRYGSLKVVNDYVRDPINIREILQAPLVIKKPEKGKSSILIFHTHT
                     SEGYCLTEEDKYNNENFTTDTEMNVVKAGSIIEKILNEKYNINTLHDKTVHDQGYNHN
                     IAYDLSKKTVESIKEEHDSIQLTIDIHRDGAFDGSKKFDPTVEHNGKMFAQVMFVVGC
                     DYDVNNPNPNWRDNFKLAMLMLEMLEEKVPGITRGISMRKDPYNTNLADRGILIEIGF
                     NGNLISEVVNTSEIVADVLGEIYSYEE"
     gene            complement(4069..8304)
                     /locus_tag="PLI11_01170"
     CDS             complement(4069..8304)
                     /locus_tag="PLI11_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPZ51505.1"
                     /translation="MRDIVHIGLDVGSTTVKIVILDREKELLYHKYQRHYSDIRRTIY
                     DMLKEALIKFKDYHATIKLTGSGGMLVAEWLGLPFVQEVVASSEAVRRLIPQTNVAIE
                     LGGEDAKITYFDDPIEQRMNGTCAGGTGAFIDQMAILLKTDAAGLNEYAKNHTIIYPI
                     AARCGVFAKTDIQPLLNEGALKEDIAASVFQSVVNQTISGLACGRPIRGNVAFLGGPL
                     NYLSELRKRFIETLVLKDEQIVFPKNSHLFVAMGAAFSSEDMEPVAIKDILDKLEHVL
                     NIQADEITRLRPLFTDENELNDFYERHNKEKAKRGDLSTYEGLCYFGIDAGSTTTKAV
                     LIGEDDEILYTYYASNFGSPFNSALNILKDLYSKMPPTAKIANSCVTGYGEGLIKTAL
                     NIDMGEIETIAHYKAAEKFLPGVEFILDIGGQDMKCLKVSDGVIHNIMLNEACSSGCG
                     SFIEAFATSLGMSVSEFAKEALSAQKPVDLGSRCTVFMNSRVKQAQKEGASVADISAG
                     LSYSVIKNALTKVIKIRSPEQMGKKIITQGGTFLNDAILRAFEIVSEREAVRPDIAGL
                     MGAYGCALIAKEHYIEGRESSILSVEDAEKIEVDIRKARCGKCPNNCLLTINRFSNGT
                     RFISGNRCERGLGASASSSTSLPNLYKYKYERLFQYEPIKDAKRGRVGIPRVLNMYEN
                     YPFWFTFFTCLGYEVVISDHSSKELYESGMETMPSESVCYPGKIVHGHIMNLIDKGVK
                     FIFYPCIPYEHKEDEKANNHYNCPIVTSYPEVIKANMDILSEKGITFMKPFLPIYDQK
                     RMGDRLYEELSPLGIEKDEIRNALEKAYEEDECFKNDVRKKGEETLEYCRKNKITPIV
                     LAGRPYHVDPEINHGIPELINSFGFAVVTEDSISHLAEIETPIRVVNQWTYHSRLYRA
                     AHYVRENNDVELVQLNSFGCGLDAVTTDQVEEILTSNGNIYTCLKIDEGTNLGAIRIR
                     IRSLKAALEERNRHGFEIKKNVSYAQKKVLFTKEMKKNHTILSPQMSPIHFDILEEAF
                     KANGYNLEVLPSNDRVAVDEGLKYVNNDACYPSILVVGQIAEALKSGKYDLDNTSVII
                     TQTGGGCRATNYIAFIRKALKDMGYGHVPVISLSASGLEKNPGFNITLPLINSAMMAV
                     CYGDLFMRLLYRTRPYEKVPGSANRLHQKWNEKVKKSVHNGRIREYIDNIKRIIHDFD
                     NLELLDIKKPRVGLVGEILVKFHPTANNFVVDLVESEGAEAVMPDLMDFLLYCAYNQI
                     AKRELLTGSRSSVFLAKTAIGVIEKYRKPMRKYLSRSKRFTPPSDIYEMAEAANKILS
                     VGNCTGEGWFLTAEMIELIEAGVPNIVCMQPFACLPNHVTGKGMIKALRREYPESNIV
                     AVDYDPGASEVNQLNRIRLMLSAAFRNLNLGEA"
     gene            complement(8329..>8690)
                     /locus_tag="PLI11_01175"
     CDS             complement(8329..>8690)
                     /locus_tag="PLI11_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HPZ51506.1"
                     /translation="RVRAKAYDIVLNGYEIGGGSIRIYDNELQKRMFKALGFSEEEAY
                     ERFGFLLDAFRYGTPPHGGMAYGLDRLVMIMANCDNIRDVIAFPKVQNASCLMTGAPG
                     MVEEKQLKELNIKIEEE"
BASE COUNT         2315 a         1951 c         1536 g         2888 t
ORIGIN      
        1 aaacatctta ggatcaggtc cttttgttaa tctaaagcca aggagcataa attcattcat
       61 cttatcggta tcacttaata cttcttcatt aaatacgtta aaatcaagac taaggtagtc
      121 tctaaaatta ctcgtatttc taaatcttat actgtttatg tatgaagatg caccaagacc
      181 aagacccaaa tattcattgc agtgccaata aagaagattg tgcctgcatt catatccgtt
      241 taatgcaaaa ttggatatct catagtgaat atattctttg gacatcaaat attccaaaca
      301 tttatggtac atttgcctgt ccaattcatc ctttggcaac ttaacaattc ccttatcaat
      361 catatcatac aaactagtct cttcctccag aatcaagctg tacgcagata tgtgaggaac
      421 atttagattt gccagctcta ttaatgtgct catgaataca tcaacgcttt gattgggcac
      481 accaactaaa acatcagcgt taatgttttc aaatccagcc ttttgagcat ttctgatggc
      541 gttgatggaa tcttcttttg tatgtattct gcctagaaat ttcgcaagat catcattcaa
      601 ggactgaatg ccgaaactaa ttctgttaat gccagcatcc ttatacatta aaaaatcatc
      661 tttttttaca gtgccagggt ttgcttcaat cgttatttca gcatcctttg caattacaaa
      721 gttttttcta agtttttcaa taagtgaatg aattagtttc ggttcaacaa tggaaggagt
      781 gcctccccca atgaatatgc tggtaatagt tttattgaca ctttctttct ttttattcat
      841 ttcaagatag agagtgtcaa aatacttttc tacgctttca ttgccaatag tattaatacc
      901 aaatgaaaga aagtcgcaat atatgcactt tctttcacaa aatggtatat ggacatatat
      961 gccaaaatct tcagcatgca tgttaatcat ccaactttag tacggtaaga aaagcctctt
     1021 gaggtatttc cacactgcca aactgcctca tacgcttttt accttccttc tgtttttcaa
     1081 gcaatttctt ctttcttgaa atgtcgccac catagcattt ggacgtaacg tcttttctgt
     1141 atgccttaac agtttccctt gctatgatct tgccgccaag gtatgcctga ataggtactt
     1201 caaactggtg tctgggaatt gcttccttaa gcttttcagc cacctttctt cccctttggt
     1261 atgctttctc ttcatgaacg ataaaggaaa gagcgtccac tacttcacca tttacgtata
     1321 tgtcaagcct tacaagcttt gaaaccttgt agccagtcat ctcataatct agtgacgcat
     1381 agccttttgt tctagatttt agtgcgtcaa agtagtcgta aatgacctcg tttagaggta
     1441 tttcgtaagt aagcagtacc ctgtcacgct caagataatc catgttaagg aacgtacctc
     1501 ttctgtcaat ggtaagctcc atgatgttgc cgacaaattc cgctggagtc agtatggatg
     1561 ctttcaccat tggttcttct atatggtcta tcatgtttac agaaggcatg tttgttgggt
     1621 tgtccagttc cagaacctca ccatttgttt tgtaaatcct gtatataacg cttggtgttg
     1681 tggtaatgat gtcgatatca aactctctct gaattctttc aagtataatc tccatatgaa
     1741 gaagtcccaa gaatccgcat ctgaaaccaa atcccaatgc ttttgatgtt tcaggttcga
     1801 aagccagcga tgcgtcatta agctgaagtt tagctaaagc atcccttaat ccttcaaaat
     1861 ctgaaccgtc gacaggataa actccgcaaa aaaccattgg ttttgctttc ttgtatcctg
     1921 gcaaaggatt cttggcagga ttgcttacta gggtaacagt atcaccaaca ttggcatcct
     1981 gtatgttttt aatgtttgcc acaatatacc caacttcccc tgtttccaag gagtcacatg
     2041 ggtagaaagt gcctggcttg aaccacccta gttcagtaat aatgaaggtt ttctctgttg
     2101 acatgagttt gattgtatcg ccttttttga gtctgccgtt tacaaccttt acatatatga
     2161 ttacacctct gtaggtgtca taaacggagt cgaaaatcag tgcctgaaga ggagcttcct
     2221 tgtcacctga tggtgatggg attctggttg ctatttcttc aaggacatcc ttaatgccta
     2281 tacctgcctt tgcagagata agtggagcat cggatgcagg tatgccaatt acctcctcga
     2341 tttccttctt tacaacttca ggttgcgctg caggcaaatc tattttgttt ataaccggga
     2401 taatttccaa gttgtgatcc atggccatat aaacatttgc cagagtttgt gcttcaattc
     2461 cctgagctgc gtccaccacc aggattgcac cttcgcatgc agccaatgtt cttgatactt
     2521 cataagtaaa gtcaacatga cctggggtgt caataagatt ataaatatac tcaactccat
     2581 catcggcaag ataattcaat ctgacggaac gcgccttaat agtaattcct ctttcccttt
     2641 ccaggtccat gttgtccaga acctgttcct ccatttccct ttcggaaagc tctccagtct
     2701 gttctaatat cctgtcagcc aatgtagatt ttccatggtc aatatgagca attatgcaaa
     2761 aattcctaat tttatcctga ttgttcatat gctactccct atctttattt attcttcgta
     2821 tgaataaatt tcacccagta catccgctac aatttcagat gtgttaacaa cctcagaaat
     2881 caaatttcca ttgaatccta tttcaataag tattcccctg tctgccaggt ttgtattata
     2941 tggatctttt ctcatggaaa ttcctctagt tatacctgga accttttctt ccagcatttc
     3001 caacatcagc atggcaagtt tgaaattgtc cctccagttt ggattaggat tattaacatc
     3061 ataatcgcat ccaacaacaa acatcacctg ggcaaacatt tttccgttat gttcaacggt
     3121 gggatcaaac tttttgcttc catcaaaagc gccatctcga tgtatatcga tagtcagttg
     3181 aatggaatca tgttcttcct taattgattc tacagtcttt ttagacaaat cataagcaat
     3241 attatgatta tacccttgat catgaactgt cttgtcatgc aaggtattaa tattatactt
     3301 ttcatttaga attttttcaa ttatgcttcc tgctttcaca acattcatct cagtgtctgt
     3361 tgtaaagttt tcattattat acttatcttc ttcagtcaaa caataccctt cacttgtatg
     3421 agtgtggaat atgaggatgg aggatttgcc tttttcaggc tttttaataa ccagaggcgc
     3481 ttgaagaatc tccctaatat taattggatc tcgaacatag tcgttaacca ccttcaagga
     3541 gccatacctg agttcactta tgtagcctgc caccagttct ttttcaatat catccttgat
     3601 tcccgtttcc acggagattt cagtatcgta gaatactgaa tatttgtatg aattgtcata
     3661 taaactgaac atatagtatt ttatgttctc ttcaacgggc ataggggtat tatttggttt
     3721 tggtggttta cttacacttg ggcttgtccc gttcaaatac aagtccacca gcttctcatg
     3781 tgtatgaatt ctttttacaa ggggcgaggc aaccccatat attctgttgg acagatacct
     3841 tccgttatag ttatgggtct ttgcctcgtc catgttttca agaagttcct tcaaagcaat
     3901 tttcccttta ttatcaacgg ttaccaatgc acatacaaca agaatagaga taaccagaat
     3961 ttcgatgata ataaagagga gcatgtattt ttttattctt ttcttctttt gtaatctgtt
     4021 attctctatt aaatacaaat gcgagctatc gtatattctt ctattcactt atgcttcacc
     4081 caaatttaga tttctaaatg cagctgacaa catcagtctg attctgttca gctggttaac
     4141 ctcactggca ccaggatcgt aatccactgc cacaatgttg ctctcaggat attcccttct
     4201 caaagccttt atcatgcctt ttcctgttac atgattgggc aggcatgcaa aaggttgcat
     4261 gcaaacaatg ttgggaactc cagcttctat aagttctatc atttcagccg tcaagaacca
     4321 tccttcgcca gtgcaattac ctactgacag tatcttgttg gcagcttccg ccatctcata
     4381 tatatcgctg ggcggagtaa agcgcttgct tcttgacaga tattttctca tgggctttct
     4441 gtacttctca attacaccaa ttgcagtttt cgcaagaaat acggatgatc ttgagcctgt
     4501 aagaagctct ctttttgcaa tttggttgta agcgcaatac aaaagaaagt ccataaggtc
     4561 gggcattacc gcctctgctc cttcactttc aactaggtcc accacaaagt tgttggccgt
     4621 tggatggaat ttgaccagta tttccccaac caggccgact cttggctttt taatatccaa
     4681 aagttccagg ttgtcaaaat cgtggattat ccttttaata ttgtctatgt actctcgtat
     4741 tcttccatta tgtacagact tcttaacctt ttcattccac ttctgatgca acctgttggc
     4801 ggaacctgga accttttcat agggccttgt tctgtaaagc aacctcatga acaagtctcc
     4861 atagcaaact gccatcatgg cagaatttat aagcggcagt gtgatgttga atcctgggtt
     4921 cttctcaagt cctgaagcag atagtgaaat gacaggaaca tggccatagc ccatgtcttt
     4981 caatgctttt ctaataaagg ctatgtagtt ggttgccctg catccgccac cagtctgagt
     5041 aataatgacg gaagtattgt caaggtcata cttccctgat ttcagagctt cggctatctg
     5101 acctactacg aggattgaag ggtagcatgc atcattatta acatacttga gtccttcgtc
     5161 caccgctact ctatcgttgg atggcagtac ttcaagattg taaccgtttg ccttgaatgc
     5221 ctcttcaagt atatcgaaat gtatcggaga catttgaggc gataatatgg tatggttctt
     5281 cttcatttct ttcgtaaaga gaaccttttt ttgggcatat gagacattct tcttaatctc
     5341 aaaaccatgc ctgtttcttt cctcaagagc cgccttgagt gacctgatcc ttattctaat
     5401 tgcgccgagg ttggtaccct cgtcaatctt caggcaggta tatatgttac cattggatgt
     5461 aaggatctct tccacctggt ccgtggtgac ggcatcaagt ccacagccaa aggagttaag
     5521 ctgtacgagt tccacatcat tgttttctct cacatagtgg gctgccctgt atagtctcga
     5581 gtgatatgtc cattggttta caacacggat tggtgtttca atttcggcca aatgcgaaat
     5641 tgagtcctcc gtcactactg caaatccaaa ggagtttatg agctccggga taccgtggtt
     5701 gatttcagga tccacatggt aaggccttcc agccagaact atgggagtta ttttgttttt
     5761 acgacagtat tcgagagtct cttcgccttt tttccttaca tcattcttaa aacattcatc
     5821 ttcttcatat gctttttcaa gagcatttct tatctcgtct ttttcaatgc ccaatgggct
     5881 caattcttcg taaagcctgt cgcccattcg tttttgatca taaattggca ggaatggctt
     5941 catgaatgta atgccttttt cacttaatat gtccatgttc gctttaatta cttccggata
     6001 agaagtgacg attggacaat tgtagtgatt gttagccttc tcgtcttcct tgtgttcgta
     6061 aggtatgcaa ggatagaata tgaacttcac acctttgtct atcaggttca ttatgtgtcc
     6121 gtgcacaatt ttaccaggat agcatacgga ttcggaaggc atggtttcca ttccggattc
     6181 ataaagctct tttgagctgt gatccgatat gaccacttca taccccaggc atgtgaagaa
     6241 agtgaaccag aaggggtagt tttcatacat gttaagtacc ctgggtatgc ctaccctgcc
     6301 tctttttgca tcctttattg gttcgtattg gaacaggcgt tcgtatttat acttatagag
     6361 gtttggaagg ctcgtagatg aacttgcaga tgctccaagt cctctttcac atctgttgcc
     6421 tgatatgaac cgggtaccgt ttgaaaacct gtttatggta agcaggcagt tgtttggaca
     6481 ttttccacat ctggctttcc ttatgtccac ctcgattttc tcagcgtcct ccacactaag
     6541 gatgctggac tccctgcctt ctatgtaatg ttccttggct atcaatgcac agccataagc
     6601 acccatcaga ccagctatat ccggtcttac cgcttccctt tcagacacaa tctcaaacgc
     6661 cctgaggata gcatcattaa gaaatgtacc tccctgagtt attatcttct tacccatttg
     6721 ctcgggagat ctaattttaa taacttttgt cagtgcgttt tttataaccg aataggacag
     6781 tcctgcggaa atatccgcaa ctgaagcacc ttccttttga gcttgcttaa ctcttgagtt
     6841 cataaataca gtacatcttg agcccaggtc cacaggtttc tgggccgaca gtgcttcttt
     6901 tgcaaattcc gagacactca ttcctaaaga agttgcaaaa gcttcaataa atgaaccaca
     6961 accagaggaa caagcttcat tcaacataat attatgtatg acaccgtcac tgaccttgag
     7021 acatttcatg tcctggccac ctatgtcaag aataaattca actccgggaa gaaatttctc
     7081 tgctgccttg taatgggcaa ttgtctcaat ttcccccata tcaatattca gtgcggtttt
     7141 tatcaggcct tcaccatagc cagttacaca ggaattggct attttcgcag taggaggcat
     7201 tttactgtat agatccttca atatgttcag cgctgaatta aatggactgc caaagttaga
     7261 tgcatagtat gtatacagta tctcatcatc ttctccaatc aaaacagcct tggttgtcgt
     7321 ggaaccagca tctatgccaa agtagcacaa accttcatat gtacttaaat caccacgttt
     7381 tgctttttcc ttgttgtgtc tttcataaaa atcgttgagt tcattttcat ctgtaaacag
     7441 aggtctgagc cttgtaatct catcggcctg gatattcaaa acatgttcaa gtttgtcaag
     7501 aatatccttt attgcgacag gttccatgtc ttctgaggaa aaagcagctc ccatagccac
     7561 aaacagatgg gaattctttg gaaaaacgat ctgttcatct ttgagaacca aggtttctat
     7621 gaagcgcttt cttagctcgg acaaatagtt aagtggtcca ccaagaaatg cgacatttcc
     7681 cctgataggt cttccacagg caagaccgct tatggtttga tttacaactg actgaaatac
     7741 ggatgctgcg atgtcttcct tcaatgctcc ttcattcaac aaaggttgta tgtcggtttt
     7801 tgcaaacact ccgcatctgg ccgcaattgg ataaattata gtatgattct ttgcatactc
     7861 gttgagccct gcagcgtcag tctttaaaag tattgccatt tgatctataa aggctccggt
     7921 tccccctgca caggtaccgt tcattctttg ctctatagga tcatcaaaat acgttatttt
     7981 tgcgtcttct ccacctaact cgatagcaac attcgtttgt ggaatcaagc gccttactgc
     8041 ttcggatgaa gcaacaactt cctgcacaaa aggcaaacca agccactcag ccaccaacat
     8101 accgccggaa ccggtcagct tgattgtggc atgatagtcc ttaaacttaa tcaaagcctc
     8161 tttcagcata tcgtaaattg tacggcgaat atcagagtag tgcctctgat acttgtggta
     8221 caataattct ttttctctat ctaaaatgac aattttcaca gtagtagagc ctacatccag
     8281 ccctatatgc acaatgtccc tcatgaattc aaatcatcct aatcaatttt attcttcttc
     8341 tattttaatg tttagctcct taagttgctt ctcttcaacc atacctggag ctccagtcat
     8401 aagacaagat gcattctgca cctttgggaa tgcaatgaca tcccttatat tatcacaatt
     8461 tgccataatc ataaccagcc tgtcgagtcc ataggccatt ccaccatgag gtggtgttcc
     8521 atatctgaag gcatccagca ggaatccgaa tctttcgtaa gcttcttcct cgctgaatcc
     8581 cagagccttg aacattcttt tttgcaactc attatcgtat atccttatac tgcctccgcc
     8641 tatttcataa ccattcaaga caatatcata ggctttagct ctgacccttt
//
LOCUS       DAONVY010000022         8560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6311_length_8560_cov_4.032687, whole genome shotgun sequence.
ACCESSION   DAONVY010000022 DAONVY010000000
VERSION     DAONVY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6311_length_8560_cov_4.032687"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PLI11_01180"
     CDS             complement(<1..1037)
                     /locus_tag="PLI11_01180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPZ51507.1"
                     /translation="MAKIFLHSDLNNFFASVECLYDPSLRDKPVAVCGNPEARHGIVL
                     AKNMLAKKKGVKTGEALWQAKEKCRDIVFVKPHYDRYLKFSELAQEIYKEYTDLIYPY
                     GIDEAWLDVTSSTGLFGSGEKMANEIRERIKYELGITVSVGVSYNKVFAKLGSDMKKP
                     DATTVITKENFMKKVWPLPVSDLLFVGSSTKRKLNLYSIKTIGDLATCDTKLLLSLLG
                     KNGIMLQNFANGHEYADDVPHLQEPPIRSIGNSTTTPRDLITDDDVRIVLYVLCESVA
                     GRMRDHDFACRTVQIYIRNKDLRSCLRQGPLDFPTQNSYMLFDKAFSLYKQHHNGDPI
                     RSIGVRACNLFV"
     gene            complement(1131..2192)
                     /locus_tag="PLI11_01185"
     CDS             complement(1131..2192)
                     /locus_tag="PLI11_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51508.1"
                     /translation="MLNFLEMKLNLLQMNSEERMYKEYYMAQNDPERLREFLESFKDD
                     SQEHLNLWIPELNPNPIECYNEEDCMSDSRQNISVKKHNRYMPLFKHRHAFFEMVYVL
                     DGIAKITINDEKVNMKTGDICIIAPEATHTVSVFNDDSIVLNILIRKSTFKETFTSQI
                     SEDSVLSQFFMRIFYSSDKNSYILFHTEDDDMLNHLLDYLIWEGLNPSEYSTNIMDNL
                     LKTIFCYLLKNHKDNSQVSKTLTTSSTDVITILNYIQQNYATISLQSLADHFNYSTSH
                     LSRLIKKHTGTTFAKILQDIKLKKGCSLLENSNLPIKDISDLIGYESVEYFTRIFKKT
                     YNMTPSEYRQSKALSNISN"
     gene            2385..3776
                     /locus_tag="PLI11_01190"
     CDS             2385..3776
                     /locus_tag="PLI11_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPZ51509.1"
                     /translation="MKLKPNILLIMCDQLRYDCLTSNIVKTPNFDRLRNRGVSFTNAY
                     SQTPVCIPARHSLISGQNAFEIGLNENSLNRKEIRYPLPRIIRDLGYYTCAVGKMHFV
                     PVREHFGFDHMYLSEEIPSHIEDDEYLKYLRDEGYGHVIEPHGKRSKTYYVPQISELP
                     KEVHTTAWTANKTIDVIEKNKNRPFFIFSSFIKPHPPFDPCKPYHEMYDVENMPLPIQ
                     VDEIIDDAILVQNGYKVDGIDKLSDYDKKKIKAYYYASVTQVDEYIGKILDCLEENNL
                     WENTLVVLTADHGEMLGDHNGYGKRTYYEESAKIPFVLSYPKVFKQGVEVNSLAILPD
                     IYATIIKAAGGEVPEISCGVDLTDVCTMENTYTRDCLYAQYGFGRRFKAMIRWDKYKY
                     IYMANGRREFLFDLEKDPLELNPIKDEEVFSEKRKELCKYFEKYGLEVPGYEYDPPVV
                     GSFLDQTPRWHLR"
     gene            complement(3768..4427)
                     /locus_tag="PLI11_01195"
     CDS             complement(3768..4427)
                     /locus_tag="PLI11_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPZ51510.1"
                     /translation="MYKINDRIIKVAIFDLDGTLLDSLGVWSDIDIKFLSKRNLEVPS
                     DYAKNISDLSFKQVAEYTVKYFNLKENVEDVMKEWFEMAVEEYSTNVLLKPYAKDYLR
                     YVKDEGVRLCVATNLPASLSVPALKNNDVMDFFDAIYQPEDVGKSKNHPDLFLYCAKE
                     LNTNPESCIVFEDIPSNIQGAKKANMLTCGVYDSYSLYYQEDLKKLSDIYITSYKELL
                     A"
     gene            4540..5820
                     /locus_tag="PLI11_01200"
     CDS             4540..5820
                     /locus_tag="PLI11_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ51511.1"
                     /translation="MTFDYGKKIEVRYDVDVCVIGGGPAGVAAGVTAARQGARVLMID
                     SNGFFGGAATAALVPAFMRFANGDVFLSGGIGREIYDACEIEKYGTGIRVEKLKRIYD
                     NMVIESGCDFLFHTNLIDVVVENGFVKYAIVSAKSGIYAIKAKVFVDATGDGDLCAMA
                     GNPYEMGDEDGKCMPATLCSIWVDIDWDNVQKPDSRELARAFADGVFTQEDYHLSGMW
                     RTGEHLGGGNIGHCYNVVATDERSLTQAMVTGRKILPEFEKYYREYLGGGYANTSVAI
                     SGSYLGVRESRRIVGEYVLNEEDYVKRASFDDEIGRYYYPIDIHPDPSKDSYDKFLQE
                     FHGLRYQPGESYGIPYRSLVPVKLANVLVGGRCISTDKKMQSSIRVMPGCYITGQACG
                     MASVLAANKDGETRNVNIRELQDKLRSIGGYLPK"
     gene            5817..6767
                     /locus_tag="PLI11_01205"
     CDS             5817..6767
                     /locus_tag="PLI11_01205"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HPZ51512.1"
                     /translation="MNLPERLVDAIRREIEGYPLSEIAETARNVSNNYRGMKGFVSGE
                     DEAKAYVAYRMPATFASLYYVFNKIVSKMPHFKPETLLDVGAGPATSVWASSLLFDSL
                     TDFKLFEHEGSMIQVGKRMLEFLDGKYNIEWHGEDITRDVDFDKSDIVVSSYMLNEID
                     KDKRESLIKRLWESTKGVLVLVEPGSRLGFGIIDELRSLLVESGGKIIGPCTHNDTCP
                     MPEEEWCHFAQRVNRPDFLRYAKGGTLPYEDEKFSYICVSKIEFESEREGVIVFPPKL
                     RKGHVILDVCTKNGIEKRTYAKKEKEVYKAAKKLNLGDEI"
     gene            complement(6812..7858)
                     /locus_tag="PLI11_01210"
     CDS             complement(6812..7858)
                     /locus_tag="PLI11_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51513.1"
                     /translation="MTTDERLNINVNDINDNIETQKVKEKCEVCELTDCSNTINFKNL
                     LVVPKEFCPVPPGCWDSLNISVKPNLHCQLSICSVLDAKLVYTDPCGKSHKIENYSIR
                     VKKARVVGCIDYIISASYALTDVDCPPASVSGFYTIHVDRVIEFLDDLDVCHCSVYIK
                     DTKVCFDNPPEFMYGNDHALKIKGEFELGLGYIRNGSFERNLAGWKLDVPSGAETRTV
                     SSFDTYEPIEGNYFALMKTNGPGAYNEMEQSFYAEPGDKICGWSFFLTNDYFPYDDNC
                     EVSLYKGNDKIATLFFASVTTVGDYGRTPWTYWEHKITEKGKYKIKAKIANVGDSSVD
                     SYMGLDAIKLTSKK"
     gene            8161..>8560
                     /locus_tag="PLI11_01215"
     CDS             8161..>8560
                     /locus_tag="PLI11_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HPZ51514.1"
                     /translation="MSFKRFARFILFILLFSIVLNITAFSEGSIEESYVFPIVENIIK
                     TKNKAFIAQDEEAVKALYDMTRTSGKWACEHEIKRIRYLKNWSDKQGVVFTDINSNLV
                     IRWTRMKDNGNMVANMLASTSYYYAYEDRPE"
BASE COUNT         2532 a         1520 c         1907 g         2601 t
ORIGIN      
        1 acaaacagat tgcatgccct cacgccaatg gatctgatgg ggtctccgtt gtgatgctgt
       61 ttgtaaaggc tgaaagcctt gtcaaaaagc atgtaggagt tttgcgtggg gaagtccaat
      121 ggtccctgac gcaaacaact ccttaaatcc ttgtttcgaa tatatatctg tacggtcctg
      181 catgcaaagt cgtgatcccg cattctgcct gcaacgcttt cgcataggac ataaagaaca
      241 attctgacat catcgtccgt tatcaaatcc cttggagtag ttgtgctgtt tcctatgctt
      301 ctaatagggg gttcctgtaa atgtggaaca tcgtctgcgt attcatgacc gtttgcaaag
      361 ttttgaagca tgatgccgtt ctttccgaga aggctcaata aaagctttgt atcacaggtt
      421 gcaaggtcac ctattgtttt tattgaatac agatttaatt ttcgcttcgt tgaagagcct
      481 acaaacaaaa gatccgaaac agggagaggc cacacctttt tcataaaatt ttcttttgta
      541 ataactgttg tggcatcagg ctttttcata tcagaaccca gttttgcaaa caccttgttg
      601 tatgatactc cgacagagac ggtaatcccg agttcatact ttattctttc ccttatttca
      661 ttcgccatct tttcaccgct tccaaacaat ccggtactgg aggtgacatc cagccaggct
      721 tcatcaattc cataagggta tataagatcc gtatactctt tatatatctc ttgggccagt
      781 tctgaaaact tcagatacct gtcatagtgg ggctttacaa acactatatc ccggcatttt
      841 tcttttgcct gccacaaagc ttctcctgtt ttgacccctt ttttctttgc cagcatgttt
      901 ttggcaagaa caataccgtg gcgagcttcg ggatttccgc ataccgccac gggtttatct
      961 ctaagtgaag ggtcataaag acactccact gaagcaaaga agttattcaa atccgagtga
     1021 agaaaaattt ttgccattga tgttcacctt aaatcttact gtcattttct ctacgtataa
     1081 tatactaata caaacaattg ttcgtgtcaa cagtaagatt ttagcgaacg ttagtttgat
     1141 atatttgaaa gagcttttga ttgcctgtat tcacttggcg tcatattata ggttttctta
     1201 aaaattcttg taaaatattc aaccgactca tatccaatca aatcacttat gtcttttatg
     1261 ggcagattgg aattctcaag aagactgcag cctttcttaa gctttatatc ctgcaagatc
     1321 tttgcaaaag ttgttcctgt gtgttttttt attagtctgc tcaagtggga agtggaataa
     1381 ttgaaatggt cagcaagact ctgcagagaa atagttgcat agttctgctg aatatagttg
     1441 agaatggtta tgacatccgt ggaggatgtg gttagtgttt tcgatacctg cgaattgtcc
     1501 ttgtggtttt tgagcaggta gcagaatatg gtcttgagca ggttgtccat tatatttgta
     1561 ctgtattccg aaggattcaa accttcccag atgagatagt caagaagatg attcaacatg
     1621 tcatcgtctt ccgtatggaa gagaatatag ctgtttttat cgcttgagta gaagatgcgc
     1681 ataaagaatt gtgaaagtac cgaatcctcg gaaatctggc ttgtgaaggt ttccttgaaa
     1741 gtacttttcc ttataagtat gttcagcaca atactgtcgt cattgaaaac gcttactgta
     1801 tgggtagctt caggggcaat tatacatatg tctcccgttt tcatattgac tttctcatca
     1861 tttattgtaa tttttgcaat gccgtccagc acataaacca tttcaaaaaa tgcatgcctg
     1921 tgcttgaaca gaggcatgta tctgttgtgt tttttaacag aaatgttttg ccttgaatcg
     1981 ctcatgcagt cctcttcatt atagcattca attggatttg gattaagttc aggtatccaa
     2041 aggtttaagt gttcctgtga gtcatccttg aagctctcca ggaattccct gagtctttca
     2101 ggatcgtttt gagccatata atattctttg tacattcgtt cttcgctgtt catttgcaac
     2161 aaattcagct tcatttctaa aaaattgagc atactatccc tgccatttta acttttgtat
     2221 ttatatcacg tattatatat tttactgtca tatcaaaaaa agtcaagttt gtgcattttc
     2281 tagcagataa attaatgtta taatatggga aaacatatcg aaatggaggc aattaaactg
     2341 tctgttaaga aaataaccaa ggtaatatag ccatggagga ggatatgaaa ttgaaaccaa
     2401 acatacttct aattatgtgt gaccagttga ggtacgactg ccttacaagc aacatagtaa
     2461 aaaccccgaa cttcgacaga cttagaaaca ggggagtaag ttttacaaat gcatacagtc
     2521 agacacctgt atgcatacca gcaaggcatt cgctaatatc cgggcagaat gcttttgaga
     2581 taggattgaa tgaaaactca ttaaacagga aagagataag atacccattg cccaggatta
     2641 tcagggattt gggatattac acatgcgctg ttggaaagat gcactttgtt cctgtaaggg
     2701 agcattttgg atttgatcat atgtatctgt cggaagaaat tccttcacat attgaagatg
     2761 atgagtactt gaaatatcta agggatgaag gctacgggca tgttatagag ccgcatggaa
     2821 agagaagcaa gacatattat gttcctcaaa ttagcgagct tccaaaagaa gtacatacca
     2881 cagcctggac tgcaaacaag acaattgatg ttattgaaaa gaacaagaac agacctttct
     2941 ttatcttttc ctcgtttata aagccgcatc cgccgtttga tccttgcaag ccataccatg
     3001 agatgtatga tgttgaaaac atgcctcttc ccatacaggt ggatgagata atagatgatg
     3061 caattcttgt acagaatgga tacaaggtgg acggtattga taaactaagt gattatgata
     3121 agaaaaagat aaaggcgtat tactacgcct ctgttaccca agtggatgaa tatattggaa
     3181 agatactcga ctgtcttgaa gagaacaatc tgtgggaaaa tacacttgta gttttgactg
     3241 cggaccatgg tgagatgctg ggcgaccata acgggtatgg caaaaggacg tattatgaag
     3301 aatcggcaaa gattcctttt gtgttaagtt atccaaaagt gttcaaacaa ggcgttgaag
     3361 taaactcttt ggcaatattg cctgacatct atgcaaccat aattaaggca gcaggtggag
     3421 aggtgccgga gatttcctgc ggtgtggatt taacggatgt ctgcactatg gagaacacat
     3481 atacaagaga ctgtctttat gcccaatacg gatttggaag aagattcaaa gccatgatta
     3541 ggtgggacaa atacaagtac atatacatgg caaacggcag gagggagttt ttgttcgatt
     3601 tggaaaagga tcctttggag ctaaacccca taaaggatga agaggtcttt tctgaaaaga
     3661 gaaaagaatt gtgcaaatat tttgagaaat atggattgga agtgcctggt tatgaatacg
     3721 accccccagt ggtcggttcg tttttggacc agactcccag gtggcattta cgctagcagt
     3781 tctttgtagc ttgtgatgta gatgtctgaa agtttcttca agtcttcctg atagtataaa
     3841 gaatatgaat cataaacccc gcaggtaagc atattggctt ttttggcgcc ttgaatgttt
     3901 gaaggaatgt cttcaaagac aatacaactc tcggggtttg tatttaactc ctttgcacag
     3961 taaagaaaca agtcagggtg atttttgctt tttcccacgt cctcaggctg gtaaatggcg
     4021 tcaaaaaagt ccataacatc attgtttttt agtgctggta cggataggga tgcgggaagg
     4081 tttgttgcaa cacacaatct tactccttcg tctttaacat agcgcaggta atccttggca
     4141 tagggcttta gcaaaacgtt ggtagagtac tcttccacgg ccatttcaaa ccattccttc
     4201 ataacatcct caacgttttc tttcagattg aaatatttga ctgtgtattc tgcaacctgt
     4261 ttgaaactga ggtctgaaat gtttttcgca taatctgaag gaacttccag attccttttt
     4321 gaaagaaact taatatcaat atcggaccag actcccaaag agtccaaaag tgttccatca
     4381 agatcaaata tggcaacttt tattattctg tcattaattt tgtacatggt gctccttttt
     4441 ttgtggtatt ataccaaaaa ggcattaata gtcaagcttg ttaaaacaat gccttagtga
     4501 tatcatacca agatgaaatc tagtacaagg agcatgaata tgacatttga ttatggaaag
     4561 aaaattgaag taagatatga tgtagatgta tgtgtgattg gtggaggacc tgctggtgtt
     4621 gccgcaggtg taactgctgc aaggcagggc gcaagagttt tgatgattga ctcaaatggc
     4681 ttttttggcg gagctgcaac agcggctttg gtacctgcat ttatgagatt tgcaaatgga
     4741 gatgtctttc tttcaggagg aataggcaga gaaatatatg atgcctgtga aatcgaaaag
     4801 tatggtacag gcataagggt ggagaagctg aaaagaatat atgacaacat ggtcatcgaa
     4861 tcgggatgtg atttcctttt ccacacaaat ctgattgatg tagttgtaga gaatggattt
     4921 gtaaaatatg cgatagtgtc tgccaaaagc gggatatatg caataaaggc aaaagtcttt
     4981 gtggatgcca caggagatgg agatttgtgt gccatggcag gaaaccccta tgagatgggg
     5041 gatgaagacg gaaaatgcat gcctgcaacc ttgtgctcta tctgggtgga tattgactgg
     5101 gataatgtgc aaaaaccaga cagtagggaa ctggcaagag cgtttgcaga cggagtattc
     5161 acccaggaag attatcattt gtctggaatg tggagaactg gagaacactt gggtggtgga
     5221 aacattggcc actgttacaa tgttgttgca acggatgaaa ggtctcttac acaggcaatg
     5281 gttacaggta gaaaaatact tcccgagttt gagaaatatt acagggagta tctgggtgga
     5341 ggatatgcaa acaccagtgt tgcaatatcg ggctcgtatc ttggcgtcag ggaatcaagg
     5401 agaatagttg gagaatatgt tttgaatgaa gaagattatg tcaagcgggc aagttttgat
     5461 gacgaaattg gaagatacta ttatccaatc gatattcatc ctgatccgtc aaaggattct
     5521 tatgacaagt tcttgcagga gttccacgga cttaggtatc agcctggaga gagctacgga
     5581 attccgtaca ggtcgttggt tcccgtaaag cttgccaacg ttctggtggg cggcagatgc
     5641 ataagtaccg acaagaagat gcagtcttca ataagggtta tgcccggctg ctacattaca
     5701 ggtcaggctt gcggtatggc ttcagtgctt gctgcgaaca aagatggcga aaccaggaat
     5761 gtaaatatta gagagcttca ggacaagttg agaagcattg gaggatactt gcccaaatga
     5821 atctgccgga aagattagtt gatgcaatac gcagggaaat tgaagggtat cctttgtcgg
     5881 aaattgccga gacggcaaga aacgtttcaa acaactacag agggatgaaa ggttttgtat
     5941 ccggagagga tgaggccaaa gcctatgttg cctacaggat gcctgcaact tttgcatccc
     6001 tatactatgt ttttaacaag attgtgagca agatgcccca tttcaaaccc gaaacactat
     6061 tggacgtggg ggcgggccct gccacgtctg tatgggcgtc ttctcttttg tttgattctt
     6121 tgactgactt taagcttttt gaacatgaag gaagcatgat acaggtagga aaaagaatgc
     6181 ttgagttttt ggacggaaaa tacaatatag agtggcatgg ggaagacatt accagggatg
     6241 tggattttga caagagtgat attgttgttt catcatatat gctgaatgag atagacaagg
     6301 ataagaggga aagtcttatt aaaagattat gggagagtac aaaaggtgtg cttgtcttgg
     6361 ttgagcctgg ttcaagacta ggttttggga ttattgatga attaagaagc ttgcttgttg
     6421 aaagcggggg caaaatcatt ggaccgtgta cacataatga tacctgtccc atgcctgagg
     6481 aagaatggtg ccattttgca caaagagtaa accgtcctga ttttctcagg tatgcaaaag
     6541 gcggaacgct tccttatgaa gatgagaagt tttcgtatat atgtgtgtcc aaaatagagt
     6601 ttgagagtga aagggaggga gttatagttt ttcctccaaa gcttagaaag ggccatgtaa
     6661 tattggatgt ttgtacaaag aacggtattg aaaaaaggac atatgcaaag aaggagaagg
     6721 aagtctacaa ggcggcgaaa aaactgaacc ttggtgatga gatatgaaac accgctcatt
     6781 gagagcggtg ttcctgaaca tataggagga gttatttttt ggaagtcaat ttaatggcat
     6841 ccagtcccat ataactgtct actgacgagt caccaacatt tgcaattttg gctttgatct
     6901 tgtattttcc tttttctgtt atcttgtgct cccaataggt ccaaggtgtg cggccgtagt
     6961 cgcctactgt ggtaacactt gcaaagaaca atgtagcaat tttatcattg cctttataca
     7021 gggatacttc gcagttgtcg tcgtatggga agtagtcgtt tgttagaaag aaagaccagc
     7081 cacaaatctt gtcgcctggt tctgcgtaaa aggactgttc catctcgttg taagctccgg
     7141 gaccgtttgt tttcataagt gcaaagtaat tgccttctat gggctcatat gtgtcaaagg
     7201 agcttactgt tctggtctcg gcgcctgatg gtacatccaa tttccaaccg gccaggtttc
     7261 tttcaaagct tccatttctt atgtaaccta atcctaattc gaactctccc tttattttca
     7321 atgcatggtc gttgccatac atgaactcag gaggattgtc aaaacaaact ttggtgtctt
     7381 taatgtaaac ggagcaatga caaacatcca aatcatccag gaactcaatt actctatcta
     7441 catgaattgt gtaaaagccg gaaaccgatg ctgggggaca atcaacgtca gtcaatgcat
     7501 aactggcact aattatatag tctatgcatc ctaccactct tgcttttttt actctaatag
     7561 agtagttctc tattttgtgg cttttaccgc atggatccgt ataaaccagt tttgcatcca
     7621 gcacgctgca gatactcagc tggcagtgca gatttggttt tacagatatg ttgagactat
     7681 cccagcatcc cggggggacg gggcaaaact cttttggtac gaccaacaga ttcttgaagt
     7741 ttatagtgtt gctacagtct gtcagttcgc atacctcaca tttttctttg actttttgtg
     7801 tctcaatatt atcattgata tcatttacgt ttatgtttaa gcgttcgtca gttgtcataa
     7861 tgacctccaa tcgtgattat attacattat atgaagggca atatggcggc ggttacaaaa
     7921 attttctata aaattatcaa aaaaatttgc acttgtttga ttcttcaggt atattatata
     7981 tgtataaagg atttcgcatg atgttgtaat gaacgaagaa caaaagcacc ttaaacgtag
     8041 atgactttta gtacgaatca tcacctgaat gcggagtcct ttattctttt atttttttac
     8101 agcataaatc aaaatcaaat gcacatatta acctctgtga ttatgattgt ggaggttgaa
     8161 atgtctttca agagatttgc aagattcatc ctttttattc tcttgttctc aattgtttta
     8221 aacatcactg ctttttctga aggaagcatt gaggagtctt atgtttttcc aattgtagaa
     8281 aatataatta agacaaaaaa caaagctttt attgcacagg atgaggaagc tgtaaaggct
     8341 ttgtacgata tgacgagaac tagtggaaaa tgggcatgcg agcatgaaat aaagagaatc
     8401 aggtatttaa aaaactggtc cgataaacag ggtgttgtgt ttacggatat caattcgaat
     8461 cttgtcataa ggtggacaag gatgaaagac aacgggaaca tggttgcaaa catgcttgcg
     8521 tccacatcct actactatgc gtatgaggat agaccggaag
//
LOCUS       DAONVY010000023         8392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6448_length_8392_cov_3.793211, whole genome shotgun sequence.
ACCESSION   DAONVY010000023 DAONVY010000000
VERSION     DAONVY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6448_length_8392_cov_3.793211"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..839)
                     /locus_tag="PLI11_01220"
     CDS             complement(105..839)
                     /locus_tag="PLI11_01220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPZ51515.1"
                     /translation="MKVTLKDKVVVVTGAGGGIGSAICEGVAKEGARIALCGGNNLEK
                     LNASADIIREYGVDPYVLPGDLANMDFLTTCIDKIAQHFGRIDILINNAGIAHNAPFE
                     ETKMEDFDRIMNINVKVPFVLCQKVLPYLRESNHATIINIASVVAHKGYVNQAIYTAS
                     KHALHGFTKSLANEVYAENIRVHTVCPGGVFTDMVKVTRPDLTGEDMIMPEEIADIVL
                     FLLTRRGNAVIDDICVRRAAKNPFDV"
     gene            complement(886..1536)
                     /locus_tag="PLI11_01225"
     CDS             complement(886..1536)
                     /locus_tag="PLI11_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51516.1"
                     /translation="MGEIIKRFESKKNSVFLVSVEGIMQVVKVHESEEKAYFESEVLK
                     RLYLYANVPKVIKVESNRIYMEYIEGKLAVDYFLECDTEEVKELARQMLSFCRVYSDQ
                     FDNFRLSDTNFRNFIVVEMDEEIELYGVDFEEREEGTLIKSAAELCAFSFLYDVDAYK
                     KKTFYNTIASNCSEQEVDFLNDYIFNSLNKLIKRRRLNLNVKSVFEELVIGNGHMV"
     gene            complement(1536..2276)
                     /gene="mobB"
                     /locus_tag="PLI11_01230"
     CDS             complement(1536..2276)
                     /gene="mobB"
                     /locus_tag="PLI11_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HPZ51517.1"
                     /translation="MKVLTVTGLSGSGKTTVIENLIKELKRRGYSIGSVKEIHYEAFA
                     IDTPGKNTYRHRQAGAEIVTARAHNETDILYPGHLPIYEVLSHYNQDYVLLEGVRDCV
                     APEIAVCKEDAEPEITPLTFAVSGRFANTGVTEYKGLPVISAVDDIGRLADLVEEKVP
                     SLMHDIEYECCGECGTDCRGFLARLLKGEVVSSDCVLSQREVKLIIDGQDIVMVPFVQ
                     RILKNAVVGVVSELNGYKEGKKIVIEIE"
     gene            complement(2279..2986)
                     /locus_tag="PLI11_01235"
                     /pseudo
     CDS             complement(2279..2986)
                     /locus_tag="PLI11_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(3006..3659)
                     /locus_tag="PLI11_01240"
     CDS             complement(3006..3659)
                     /locus_tag="PLI11_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51518.1"
                     /translation="MIELIDVSFAYNKNNMVLRNITYTFKEGVLYVLGGKNGSGKTTM
                     GKLMCGLVKTKQGIVKVSGTNIYKKTVGEISEHVGYLYQNPDLQLFAPTVYEELVFPY
                     ELNGTLTYEIKERAEKLLQVFKLEDKKDTFPLLMSLGERQRLALATIMMRDTKFIIFD
                     EPTSSIDAECKAMIHEFILSFVENGGGAFIISHDEDFNLNGVNGMNLRMEGGMLYEK"
     gene            complement(3643..4308)
                     /locus_tag="PLI11_01245"
     CDS             complement(3643..4308)
                     /locus_tag="PLI11_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51519.1"
                     /translation="MKLTVENLTVMFKNRIILDNVSLQVEAGEIVAVVGPNGSGKSTL
                     CTAIAGVSQNYGLSTVGRIRIGDKELSQLSIHERCALLGVIFQNSDNFLFSQYVEDEL
                     RFAPENLCVPRAEIETRIKEALDFTGISYLRHRKIQELSGGEKKLVSIASVLTMMSKF
                     LIADEITSGVDEDRKVQIREILRKLAKDNIGILMISHNRNDIEIADRIYVLKDGKLHD
                     RTD"
     gene            complement(4305..4853)
                     /locus_tag="PLI11_01250"
     CDS             complement(4305..4853)
                     /locus_tag="PLI11_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51520.1"
                     /translation="MKNRNVLIALVILVVLSILFGVMLFVFMNENKKNVEQYEKREIA
                     ITINGEREVYTFEELMEISQPVDFKAIYKPSNKEPIEKTYTGIELRDLLEKLGADLEN
                     MKQITFTAKDGFQKVYRAEDVRKETNVFITHLVNGKPFNEGIDVMAYEKEQEDGGPFV
                     VIKAEDKVSQNRVKMLVEIVVE"
     gene            complement(4843..6288)
                     /locus_tag="PLI11_01255"
     CDS             complement(4843..6288)
                     /locus_tag="PLI11_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPZ51521.1"
                     /translation="MKRALLILTVVLLVIFAGCTNKESEPGKYITVNGDIEGVSVEVN
                     LSDISKEEIAIKDTLYKGVRLSKVLEKVSLLAPVDKSYVMITAIDGVSALIDAETANL
                     CHVVEVEGVLNIKAPEHPRVVGIKHVLEITIVAHEDVEISDENSLKIVDQEGDRRISF
                     GNCKLLMFEKSGESRQNGNLAVKYVKKAPLKAEEFIGDDFVLYFDNYDMLKVDRDYAG
                     VFVWENGRIYYETDERYDSPVTGIVTGVDTVIFDAFDMMKILLDKNEMVMFILPDGLS
                     YEQVEYYKDNLTILSGNYKKAASVNPAISNVALAAIITGQSPFATGITKREVKAPNVK
                     DIFEYVVSKGKTASYIEGNSVLVVTSEKPKLNVANSEGYTDKGVFETAMYELGKNPDF
                     LFIHFHGIDDVNHEYSPLSEEAKQKIYEIEGYIKELVKNFYGHVIIVPDHGAVTIEDT
                     DGTKRGKHSLFKYGDMYVPFYYFERGSLIEE"
     gene            complement(6285..6851)
                     /locus_tag="PLI11_01260"
     CDS             complement(6285..6851)
                     /locus_tag="PLI11_01260"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPZ51522.1"
                     /translation="MRKVIKSYSVLDLVLIAMLAALATGFKTVANTLVKLITGPLGIP
                     GGALAGGFYMMWLAIGHSVVRKFGVSFMISLVQALVLFTTSAPGSHGIWTFITYLIPG
                     LCVDIVFLFARKNNANVLHFMIAVMMANVLGTLGSFLLWFRLSWLWLAFSLTAAALSG
                     AIGGLVAYSVDKGIRRIGLIFKEDGADQ"
     gene            complement(6848..8044)
                     /locus_tag="PLI11_01265"
     CDS             complement(6848..8044)
                     /locus_tag="PLI11_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51523.1"
                     /translation="MKKHLLILAIFVLVLALAAGCANNNGQNTTPTPTDAPQSYEDDD
                     YILEIVGIGDETIKVTKGQIRELAAGGKLVEYGEDQPAYASDKTDDDGNKIPHTLKGV
                     YFDDILEVYADDAKTSDFTSMSIYAEDGYETILSADVFNVEQGGVKMIVAFEYDGIEL
                     NPNEKSGALRVVFPDKPANTWAKQVSKIVLASEEDEETVPETESVYFSESTGDKYDGS
                     YEVDGTTYYGTSFKKIFDDGILKSGDKAQMFVYSWDGTEYTSLKTKEYYEDAFLVYAS
                     KDADSDEFAQEKDAPVFNGANILKGMKVKHTMYVTVENTSFAVLDKVFEKLDSKGKGS
                     ILVSELLDEINMKEASIYTITGVDGTEKQAKKEDLKDIRIESTDEGYVVSVDGSEFVI
                     LSIQAK"
     regulatory      complement(8135..8257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT         2310 a         2020 c         1338 g         2724 t
ORIGIN      
        1 aacgatccga ttaaagatgg cgacaggata aaaatatttc ctgtcataat tgcgggataa
       61 aagcaaagag gaaaaaactc cattatggtt ttttcctctt tttttcatac atcaaatgga
      121 ttttttgcag ctctccgcac gcagatatcg tcaatgacag cattgcctct tcttgtaagg
      181 aggaatagga caatatccgc aatctcttca ggcataatca tatcctcacc agtcagatcc
      241 ggacgtgtta ccttaaccat atcggtaaat actccacccg gacaaacagt atgaactcta
      301 atattttcag catagacctc gtttgcaagc gattttgtaa acccgtgcag ggcatgcttg
      361 gatgcagtgt atattgcctg atttacatac cctttgtggg caacaacaga tgcaatgttg
      421 ataattgtag catgattcga ttcacgcaaa tatggaagca ccttctggca taaaacaaaa
      481 ggcaccttta cgtttatatt cattatcctg tcaaaatcct ccatttttgt ttcttcaaat
      541 ggagcattgt gtgcaattcc ggcattgtta ataagtatgt caattctgcc aaaatgctgt
      601 gctattttgt caatgcaagt tgtcagaaaa tccatgttgg caagatcacc tggaagaaca
      661 tatgggtcaa caccgtattc cctaataatg tccgctgagg cattcagctt ttccaggttg
      721 ttgccgccgc aaagtgcaat cctcgcaccc tctttagcca ccccctcaca gatggcgctg
      781 ccgatgccgc ctcccgcacc agttacaact acaaccttgt ccttgagtgt taccttcatg
      841 ccatctcact cccttttcgc atattcaaac tctatggatt atatattata ccatatgccc
      901 gttgccaatt accaattctt caaagacact tttaacattt aaattcaatc tgcgtctttt
      961 aataagctta ttcaagctat taaaaatata gtcgtttaaa aagtcaactt cctgctcgga
     1021 gcaatttgat gcaattgtat tgtaaaaggt tttcttctta taagcatcaa catcatacaa
     1081 aaacgagaaa gcacacagtt cagctgcact ttttatcagt gtgccttctt ccctttcctc
     1141 aaaatcaaca ccgtacaatt caatttcttc gtccatttca accacgataa aattccggaa
     1201 gtttgtatcc gacaaacgga agttatcaaa ttggtctgag taaacccggc aaaaggatag
     1261 catctgtctt gccagttctt ttacttcttc agtatcgcat tcgagaaagt agtcaacagc
     1321 aagttttccc tctatgtatt ccatgtagat cctgtttgat tctaccttaa taacctttgg
     1381 tacgttagca tacaagtaaa gcctttttaa aacctctgat tcaaagtagg ctttttcctc
     1441 tgattcatgc acctttacaa cttgcatgat tccttccaca cttactagaa aaacactgtt
     1501 tttcttgctc tcgaaccgct taataatttc gcccatcatt ctatctctat tactatcttc
     1561 tttccttcct tgtacccatt aagttccgat acaactccca ccactgcgtt tttcagtatc
     1621 ctttgaacaa aaggcaccat tacaatatcc tgaccgtcga taatcaactt gacttctctt
     1681 tgactcagta cgcaatcgga actgacaacc tctcccttca gaagccttgc aagaaatcct
     1741 ctacaatccg taccgcattc accacagcat tcatactcta tatcatgcat taaactcgga
     1801 accttttcct cgacaaggtc tgccagtctt cctatgtcat caactgctga tattacagga
     1861 agacccttgt actccgtaac accggtgttt gcaaaccttc cacttactgc aaacgtaaga
     1921 ggagttattt caggctcagc atcttccttg caaacagcaa tctccggagc cacacagtcc
     1981 cttactcctt caagcaaaac atagtcctga ttatagtggg agagcacttc gtaaatcggc
     2041 aggtgtccgg gatacaatat gtctgtttca ttgtgagctc ttgctgttac aatttctgct
     2101 cctgcttgtc tgtgtctgta ggtgttcttt ccaggagtat caatggcaaa cgcctcataa
     2161 tgaatctcct ttacacttcc tatggaataa cctcttcttt ttagttcttt tatcaaattc
     2221 tctattacgg tggtctttcc gcttccgcta agccccgtta ccgttaatac tttcataact
     2281 acctccttgc aaacataaag tatatgatta gtaaaaatat tgatgatgca attatcattg
     2341 cgatatagtc tgctaatttt agtttcaata ctcttaaatt ggttctatca ggatacgccc
     2401 tgaatcccct tgtctccatg gatgaggcaa tatccctgct cttgataatg ctgccggaaa
     2461 ttgcaggcac caaaatgtgt gaatagattt ttatcttttt tgacagcttg agtttcttaa
     2521 tatttattcc tctcaaactt acggctttta ttctatcggc aaactcctaa ccaaacagtg
     2581 gaataaactt gagtgctatt cctgtcataa aggccagctc ataaggaatc ttgagttttg
     2641 caagtccgtc tatcatatca cgactgtcat gttttgtacc gagcatgacg gccagcatta
     2701 tgttgcccat cctgagtaca aaagacaagc tgtccacaag tcctttggaa gttacaatat
     2761 ttgcaccgag caaagtaata attggtctcc ctcccgaagt tgtgaggctt tggaaaaagg
     2821 ctatgaagat tatcattgac acaagccttc tcattctgta cagagtcttc aatggattta
     2881 atctgaatac aatgtttaac aacagggtca ataaaaagac cagcagcagc caatatacat
     2941 ttgaggcaag gacggacagt gtggatgtca gtacaataaa caacatggtg attcttggat
     3001 ccagtctact tttcatacag cattccacct tccatcctca gattcatgcc gtttactccg
     3061 ttcaaattga aatcctcatc atgtgatatt ataaaagctc ctcctccatt ttcaacaaag
     3121 cttaatataa attcatgtat cattgctttg cattccgcat caatggagga tgtcggttca
     3181 tcaaatataa tgaatttggt gtccctcatc attattgtgg caagagcaag cctctgtctt
     3241 tctcccaagg acatgagcag aggaaaggtg tcctttttgt cttcaagctt gaagacctgg
     3301 agcagctttt ccgccctctc ttttatttca taagtcagtg ttccattcag ttcataagga
     3361 aaaacaagtt cttcataaac ggtcggtgca aataattgca aatcaggatt ctggtacaaa
     3421 taccctacat gttctgatat ctcgccaaca gtctttttat atatattggt tccgcttacc
     3481 ttgacaatac cttgttttgt ctttacaagg ccgcacatta gttttcccat tgtggttttg
     3541 cctgagccgt tttttccgcc caaaacatac agtacacctt ctttgaatgt atatgtaata
     3601 tttctcagaa ccatgttgtt cttgttatac gcaaaggata cgtcaatcag ttctatcatg
     3661 caatttgcca tcctttaata catatattct gtcagcaatt tcaatatcgt ttctgttgtg
     3721 ggatatcatt agtattccaa tattgtcttt ggctagtttt cttagaatct cccgtatctg
     3781 aaccttcctg tcctcgtcta ctccggaagt tatttcatcc gctattagga atttgctcat
     3841 catggttaaa acagacgcaa ttgaaaccag cttcttctcc cctcccgaaa gctcctgaat
     3901 ttttctatgt cttaaatagc taataccggt aaaatccaaa gcttctttta ttcttgtttc
     3961 aatttccgcc ctgggaacac atagattttc aggagcaaac cgaagctcgt cctcaacata
     4021 ttgagaaaaa agaaagttat cagaattctg gaatatgaca ccaagcaagg cgcatctttc
     4081 atgaatactg agttgggaca actctttgtc acctatcctt attctgccaa cagtactcag
     4141 tccataattt tgagacacac ctgcaattgc agtacaaagt gtggattttc cactgccatt
     4201 tggtccgact actgcaacaa tttctcctgc ttcaacttgt aatgatacat tatcaagtat
     4261 aattctgttt ttaaacatta ctgttagatt ttctactgta agtttcattc caccacaatc
     4321 tctacaagca tttttactct gttctggctt actttgtctt ccgcctttat taccacaaaa
     4381 ggtccgccat cttcctgttc cttttcatat gccatcacat caatgccttc gttgaaaggc
     4441 tttccattca ccaaatgcgt tataaataca ttggtttcct tccttacatc ctctgcccgg
     4501 tacactttct gaaaaccgtc ttttgcagta aatgtaatct gtttcatgtt ctccaagtct
     4561 gcccccaact tctcaagcag gtccctcaat tctattccgg tataggtctt ttctatgggt
     4621 tccttgttgc taggcttgta tattgcctta aaatcaaccg gctggcttat ctccatcaat
     4681 tcttcaaagg tatatacttc cctctctcca ttgatggtaa ttgcaatttc ccttttttca
     4741 tactgctcca cgtttttctt gttttcattc ataaagacaa acagcataac accaaacaga
     4801 atagaaagta caaccaatat aaccagtgca ataagcacat tcctattctt caatcaaact
     4861 acccctttca aaatagtaga agggcacata catgtctcca tacttgaaca agctgtgttt
     4921 tcctcttttt gtcccatccg tgtcttctat agtcactgct ccatggtcag gcacaattat
     4981 cacatgccca taaaagtttt taaccagctc tttaatatat ccttctattt catatatctt
     5041 ctgcttggct tcctctgaca aaggagagta ctcgtgattt acatcatcaa tgccgtggaa
     5101 atgtatgaat aaaaaatcag gatttttgcc cagttcatac atggctgttt caaatacgcc
     5161 tttatcagta tatccttccg agttggctac attcagtttt ggcttttccg aggttacaac
     5221 caatactgaa ttcccttcaa tatatgatgc cgttttccct ttactgacaa cgtattcaaa
     5281 aatgtccttt acgttaggag cttttacttc cctctttgtt atgcctgttg caaaaggtga
     5341 ttgtccggta attattgctg ccaaagccac atttgatatg gctgggttca cgctggctgc
     5401 ttttttgtaa ttgcctgata atattgtaag gttatccttg taatattcca cctgctcgta
     5461 agacaacccg tcaggcagga taaacataac catttcattc ttatccaata atatcttcat
     5521 catgtcaaag gcatcgaata taacggtgtc gacgcctgtc acaatacctg taacaggact
     5581 atcgtacctc tcgtccgttt cataatatat tctgccattt tcccaaacaa aaacacctgc
     5641 ataatcccta tccactttca gcatgtcata gttgtcgaag taaagtacga aatcatcgcc
     5701 gatgaattct tccgccttta atggcgcttt ctttacatac ttcacagcga gatttccgtt
     5761 ctgtctactt tcacctgatt tctcaaacat aaggagcttg cagtttccaa aacttatacg
     5821 tctgtcaccc tcttgatcca caatttttag agaattctca tcagaaattt ccacatcctc
     5881 atgggcaact attgtaatct caagaacatg cttgatacca acaaccctcg gatgttccgg
     5941 agcctttatg ttcaaaactc cttctacttc gacaacatga cacaagttgg cagtctccgc
     6001 atcaattagt gcagacactc cgtctatggc tgttatcatt acatagctct tatcaacagg
     6061 tgccagtagt gaaaccttct caagaacctt tgacaaacgc acccctttgt aaagtgtatc
     6121 ctttattgct atctcttctt ttgaaatatc actcaagttc acttctacag agacgccttc
     6181 tatatccccg tttacggtta tgtatttgcc tggttcactt tccttgttcg tgcatcctgc
     6241 aaaaatcacc aacaagacaa ctgtcagtat caatagtgct cttttcattg atctgcccca
     6301 tcctctttga aaattaaacc aattctccta attcccttat caacagaata tgccacaagc
     6361 cctccaattg ctccggataa agccgctgca gtcaggctaa acgcaagcca caaccaggat
     6421 aacctgaacc atagcaaaaa gctccctagt gtgcccagta catttgccat catcacggca
     6481 atcatgaaat gcagaacatt ggcattgttt tttcttgcaa agagaaacac aatgtcaacg
     6541 caaagtccgg gaatcaaata cgttatgaaa gtccagatgc cgtggctgcc tggtgcgctg
     6601 gttgtaaata gtacgagagc ctggacaagg gatatcatga aagatacccc aaatttacga
     6661 actaccgaat gcccaatggc aagccacatc atataaaatc cgcctgccaa agcccctcct
     6721 gggattccca aaggacctgt tattaacttg accagtgtgt ttgcaacagt cttgaagcct
     6781 gtagcaaggg ctgctagcat cgctattaat accagatcca aaacagaata tgatttaata
     6841 accttcctca tttcgcctgt atgctcaata ttacaaactc actgccatca actgaaacaa
     6901 cgtatccttc atctgttgac tcaatcctta tatcctttaa atcttctttc tttgcctgtt
     6961 tttcagtgcc gtcaaccccg gttatggtat agattgacgc ttctttcata tttatttcat
     7021 caagcagctc actaacaaga atactgcctt tgcctttgga atcaagcttt tcaaaaactt
     7081 tgtcaagtac tgcaaaagaa gtgttttcaa cagtcacata catggtgtgt tttaccttca
     7141 tgccttttaa aatgtttgcc ccattgaata ccggtgcgtc cttctcttga gcaaactcgt
     7201 cactgtcagc atcctttgat gcataaacca agaacgcatc ctcgtaatac tcttttgtct
     7261 tcaagcttgt atactcggtt ccatcccacg aatatacgaa catctgggcc ttatcaccgc
     7321 tcttcaatat tccgtcgtca aatatttttt tgaagcttgt tccataatat gtagtgccgt
     7381 ctacctcata actaccgtca tatttgtctc cggttgattc tgagaagtat acgctttcag
     7441 tttccggaac tgtttcctca tcttcctcac ttgccaaaac tatcttggaa acctgttttg
     7501 cccaggtatt tgccggcttg tcaggaaaaa caactctcaa agctccgctc ttctcatttg
     7561 gattaagctc aatgccgtca tactcaaagg caacaatcat tttaactccg ccttgttcta
     7621 cgttgaagac atcagctgaa agaattgtct cgtaaccgtc ttcggcataa atagacatgc
     7681 ttgtaaaatc ggaagttttt gcgtcatctg cataaacttc aagtatatcg tcgaaatata
     7741 ctcctttcaa tgtgtgtgga attttgttgc catcatcgtc agtcttgtcg gatgcgtatg
     7801 caggctggtc ttcgccgtac tcaacaagtt tgccacccgc ggcaagttcc ctgatttgcc
     7861 cttttgtcac tttgatggtt tcatccccga ttcctacaat ttcaagaata tagtcgtcat
     7921 cctcatagga ctggggtgca tcagtaggtg ttggtgtggt gttctgaccg ttattatttg
     7981 cacacccagc agccagtgct aaaactaata caaatatcgc caagattaat agatgctttt
     8041 tcattttatt cttctccttt taatttcttt atcaaaaaaa attctcttga ttccgtccgg
     8101 aacaagagaa cataaagaaa ttaccaccca taagggtgat ttctttcctg ttccttttca
     8161 actccggaag gctgaaaaat ttctctttca accctatcgg tgtttcccca tggccatttt
     8221 ggctttaggt agaaaacact cggaaccagt atttttttca ctttaatatt gttaaaaaaa
     8281 tatcaaagca tttagatttt atactaaccg attaccattg tcaatatatc aggctaaaat
     8341 tacatgaaag aataacgttt ttcaggacaa aatacaactg tacggtaatt cc
//
LOCUS       DAONVY010000024         8297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6542_length_8297_cov_3.419922, whole genome shotgun sequence.
ACCESSION   DAONVY010000024 DAONVY010000000
VERSION     DAONVY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6542_length_8297_cov_3.419922"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..675)
                     /locus_tag="PLI11_01270"
     CDS             complement(85..675)
                     /locus_tag="PLI11_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPZ51524.1"
                     /translation="MIFKFNVEDFKASYVLKDITKKFNGNVVLDNISLEMPEKGILVI
                     SGYSGIGKTTFLRIMANLIAPDSGERIGFDNKRIAYLFQEDRLLPWFDLLTNITCVLQ
                     GEDKEQKAKYWLDQVGLANQYAKYPSELSGGMKRRAALARMFAFGGDLYILDEPFKGL
                     DKELKDRIIEITKNACKGKMLVVVTHDKQEEDLLLK"
     gene            complement(672..1454)
                     /locus_tag="PLI11_01275"
     CDS             complement(672..1454)
                     /locus_tag="PLI11_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPZ51525.1"
                     /translation="MKIFTTRDSKINKFLRTVLVLTVYFLIWALLAGMVDEEILIPKP
                     ASVFKSLLALSRTVLFWRSILLSIGRILIGFFLALVVGTIFAILTVKSKLVMDLFYPL
                     ISSVKATPVASFIILALFWLTTGRVPIFICFLMVLPTVWTNIREGILNVDEDLVEMSR
                     IYNFTPYARFRYIYVPTIIPFFRAAFASGLGMAWKAGVAAEVIGRPDLSIGKELYNAK
                     IYLDMPKMFAWTFVVILISIILEKLLIFLMNHIGRRYGGYEV"
     gene            complement(1429..2388)
                     /locus_tag="PLI11_01280"
     CDS             complement(1429..2388)
                     /locus_tag="PLI11_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ51526.1"
                     /translation="MIKKIVPLVIVLAMLMTACTKADKQDKTTINVAALKGPTGMGMI
                     KLIDDADKGNTKNEYKITLAGSPDEITGKIVSGEIQIAALPINLAANLYNKTNGKVQL
                     LALNTLGVLYVLEKGEEINSVSDLEGKKIFATGQGATPEYILDYILKENNVNADVEFK
                     TEHSELAALALSGNADIVMLPEPFVTNVLSKEAGFRVALDLTEEFERVSDGTVLSMGC
                     IVVNKEFAEKNKQAVKDFLDEYKASTEFVNNNISEASDLIAKYGILASKELAEKAIPN
                     CNIVFIEGGEMEQKIKGFYELLNEYNANSIGGKLPGEDFYYKG"
     gene            2593..3627
                     /locus_tag="PLI11_01285"
     CDS             2593..3627
                     /locus_tag="PLI11_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPZ51527.1"
                     /translation="MRFRPRNKADKKIIKKALQDRSRTYESNGITKFPVNSDRKKDLN
                     LLFSDIFPQKSAAYAVVSSAVIISGFLFYISDLKMSVDELNKKNQMLIEKNSQLNNNL
                     ENITNSIVKLDQETKDHMEIIQKQKEELSEIEKNFEDISSEKDAALQEILDMIKNLDI
                     FSGLITRSGNMYTASNQIQEAKLIIKQTLGDSEEAQDLLAKLDKEKDKIEDYRRRYPD
                     YRPVSGRLTSRFGYRRDPFTGETRFHNGIDIGAITGTPIYSAAYGTVIYTGFNSSSGN
                     HIIIDHGNGYKTYYKHLSKIEVKTGTVVNKGQRIGQVGSTGRSTGPHLHFEIHLYGTI
                     KNPLEYVYYN"
     gene            complement(3667..3807)
                     /locus_tag="PLI11_01290"
     CDS             complement(3667..3807)
                     /locus_tag="PLI11_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51528.1"
                     /translation="MSSKLNRIAYRRYKREITRPYIKTVARIILIPAISLLTLYILYI
                     ID"
     gene            3977..6118
                     /locus_tag="PLI11_01295"
     CDS             3977..6118
                     /locus_tag="PLI11_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPZ51529.1"
                     /translation="MQKKSKKVNKNEKKTPKVAKAFLKTLSVAIKTTIAMALVIACVL
                     GGLLIGVVAGCIITTDTLDEEDLYITGFTSYIYDADGNVIAELKGSDNINRSEVDIEV
                     VPKHLLNAFIAIEDERFYTHPGVDVKRTVGAFLGYFIPGMSSHGGSTITQQMVKTITG
                     DDARSVPRKIREQWRAYQLEKDLSKDEILELYINIIYMGHDLYGVKTAAKAYFEKDIT
                     ELNLAECAFLAGITNNPGKYNPLTTLGRENAYKRQVIILDQMLKLDFITDEEYIEAIQ
                     TELVFNEDYRKEASANSRHSYFVDTVITDIRNDLIAMGYTRKQATNIIYNTGVHIYTT
                     QNTKIQKIVEEEYCKIENFPINETILDPEAQAQSAIVVMDPRAGHVIAIYGGYGEKNK
                     NLTFNRATSAYRQPGSAIKPLLIYGPQIDKHIITTGTVLEDKEVFLDPQRPDVPWPTN
                     SGGWHAGLITARKALVRSSNICAILLYKDNVTLGLNYLKKLGIDRTEETQLAAGLGGF
                     SRGVTPLQMAAAYSAFANSGMYIEPITYTKVVDRNGNVIINKTQKSHIVFEDDKTPYI
                     MTSILNHVITDYQGTGGRAQVYNNKGEKIPTAGKTGTTNKNYDYWFCGYTPYYTAAVW
                     YGYDNLKSITSSEYGSAARLFGTVMSKIHSDLEAIPFETSPDLVYMDICNMSGGIPHS
                     GCHAANRIVANEMFIRGTEPTEECAYHEPLE"
     gene            complement(6159..7424)
                     /locus_tag="PLI11_01300"
     CDS             complement(6159..7424)
                     /locus_tag="PLI11_01300"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPZ51530.1"
                     /translation="MNVQEYVKDYGRRAKSASRVLAVTDTVKKNRALELAAEKIKENR
                     DYLIKENEKDIEYAINNGISSAMVDRLRLTDKRIDDMATGLIQLANLPDPIGEVIHGT
                     VRPNGLEILKKRVPLGVIGIIFESRPNVTADAAGLCVKSGNACILRGGSEAVNSNIAI
                     AKLFREALKDAGLPEDCVNLIDNTDRAIVNALMKLNKYVDVLIPRGGKGLINNVVMNS
                     TVPVIQTGEGICHLYVDCNADLDMALNVTMNAKIQRPSVCNAIETLLVSKTVADKFLP
                     VVVKELMEKNVEVRVCSISKEILGNMEGVKDADDSDWDTEYNDLVLSIKTVAGIEEAI
                     EHINSHGTMHSESIITTSYDKARKFQSEVDASSVYVNCSTRFTDGFEFGLGAEIGISN
                     QKLHARGPMGLYELTTVKYLINGKGQIRE"
     gene            complement(7402..8241)
                     /gene="proB"
                     /locus_tag="PLI11_01305"
     CDS             complement(7402..8241)
                     /gene="proB"
                     /locus_tag="PLI11_01305"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPZ51531.1"
                     /translation="MNIDCREARNSLKNATRIVIKIGTSSLVHSNGRINFRRIGILAR
                     VISDLMNQDKEVVLVSSGAIGIGVSKLKLKERPKTIAEKQAVAAVGQSSLMQVYSQIF
                     SEYGHAVAQILLTKDVIDRSCMKANAINTFEQLAKMKILPIVNENDTVAIDEIKFGDN
                     DNLSYLVAKLIKADLLIILTDIDGYYNKNPHENPDAILYHNITDLSEKIEEAAGGAGE
                     HGTGGMLTKVHACRLAAESGINAIIANGNNPEIIYRLLEGEDLGTLFVRKEVCDNECS
                     GVC"
BASE COUNT         2562 a         1805 c         1476 g         2454 t
ORIGIN      
        1 tttgtggttc aaaagttttc tccaaattat tccctccaaa atccaattta ttttatttta
       61 aataagtatt tgtccagtgt caacttactt aagcagtaaa tcctcttcct gtttgtcatg
      121 agtgacaaca acaagcattt tacccttgca tgcattcttg gtaatttcaa taattctatc
      181 cttcaattcc ttatcaagtc ccttgaaagg ttcatccagg atatacaagt ctcctccaaa
      241 agcaaacatt ctggcaagag cggctcttcg tttcattcca ccgctaagtt ccgacggata
      301 ttttgcatat tgatttgcaa gaccaacctg gtcaagccag tattttgcct tttgctcttt
      361 gtcttctcct tgcagaacac aggttatatt tgtcaataga tcaaaccagg gtagcagcct
      421 gtcctcctga aaaagataag caattctttt gttatcgaaa ccaatccttt caccggaatc
      481 tggtgcaata aggttagcca ttattcttaa gaaagtggtc ttgccaatac cagaataccc
      541 acttattaca agtattccct tttcaggcat ctccaaactt atattgtcaa gaacaacatt
      601 tccattaaac tttttggtaa tgtcttttaa tacatatgag gctttaaaat cctcaacatt
      661 gaatttgaaa atcatacttc atagcctcca tatctcctgc caatgtggtt catgagaaag
      721 attaacaatt tctcaaggat aatgcttatg agaataacaa cgaaagtcca ggcaaacatt
      781 tttggcatat ccagataaat ctttgcattg taaagttcct ttccaatgga taaatcaggt
      841 ctgccgatta cttctgcagc aactcctgcc ttccatgcca ttccaagtcc tgaagcaaac
      901 gcagccctga aaaaaggaat aatagtagga acatatatgt atctaaacct ggcatacgga
      961 gtgaagttat atattcttga catttcgaca aggtcttcgt ccacatttag tatgccttct
     1021 cgtatattcg tccatactgt tggcagcacc attaggaaac atatgaagat tggtactctg
     1081 ccagttgtaa gccaaaacag tgcaaggatt atgaaggagg cgacgggtgt cgccttcaca
     1141 ctgctgatta aaggataaaa caagtccata accagcttgc tctttaccgt taatatggca
     1201 aatatggtgc caactacaag ggcaagaaag aatcctatca gtatacgtcc aatggataat
     1261 aaaatggaac gccagaataa tacggttctt gaaagagcca gcaagctttt aaaaacactt
     1321 gcaggtttcg gtatcagtat ttcctcatca accattccgg ccagcaaagc ccatattaga
     1381 aaatatacag tcaagaccag gacagttcgc aaaaatttat ttatcttgct atcccttgta
     1441 gtaaaaatct tcaccaggca gttttcctcc aattgagttt gcattgtatt cgttcaacag
     1501 ttcataaaat ccttttattt tctgttccat ttcgccgcct tcaataaaca ctatattaca
     1561 attaggtata gctttctctg caagctcctt gcttgcaaga ataccatact ttgcaatcaa
     1621 atccgatgct tcggatatat tgttgtttac aaactcggtt gacgccttgt attcatcaag
     1681 aaaatctttt actgcctgtt tgttcttctc ggcaaattcc ttgttcacta cgatgcaccc
     1741 cattgaaaga actgttccat cacttaccct ttcaaactcc tcagtcaaat ccaaagctac
     1801 tctaaagccc gcttcctttg aaagaacatt ggttacaaag ggttcaggaa gcattactat
     1861 gtctgcatta ccagacaatg caagtgctgc aagttcggaa tgctctgttt tgaattccac
     1921 atctgcattt acattgttct ctttgagaat ataatcaaga atatactccg gtgtggcacc
     1981 ctgtcctgta gcaaatatct ttttgccttc caaatcggaa acagaattaa tctcttcgcc
     2041 tttttcaagt acatacaaga cacccagggt gttcaaagcc aatagctgta cttttccgtt
     2101 tgttttgtta taaaggtttg ctgcaagatt tataggcagt gcagctattt gaatctcgcc
     2161 acttacaatc ttacctgtaa tctcatcagg tgaacctgca agggtaattt tatactcatt
     2221 cttggtgttg cccttatcag cgtcatcaat taacttgatc atgcccattc cggtaggtcc
     2281 cttcagtgca gcgacattta ttgttgtttt atcttgttta tcagctttcg tacaagctgt
     2341 catcaacatt gccagtacta taactaatgg tacgattttt ttaatcattt cctaaccttc
     2401 cttcattcta tactatacct tactattata cctcttttgt catggttttc aagttgacac
     2461 acatccagtc attatgggaa atattatgaa aaatttatta atatttttat aattacccct
     2521 ttacaaagac aaaaaaacgt gatatgatac ttctggtttt tagatactta cattttgaga
     2581 ggattaatac ttatgcgctt tagacctaga aataaagcag acaaaaagat tattaagaaa
     2641 gctcttcagg acagaagtcg tacatatgaa tcaaacggga ttacgaagtt ccctgttaat
     2701 agtgaccgca aaaaagacct caatttattg ttctcagata tatttcccca gaaatcagct
     2761 gcatatgccg tagtatcgtc agcagtaatt atatcggggt ttctttttta catatctgac
     2821 ctaaaaatga gtgtcgacga actaaacaag aagaatcaga tgttgattga aaagaacagc
     2881 caattgaata acaacctaga aaacattacg aattcaattg tcaaacttga tcaagaaaca
     2941 aaagatcata tggaaataat tcaaaagcaa aaagaagaat tatctgaaat tgaaaagaat
     3001 tttgaagata taagctctga aaaagacgct gcactgcaag agattcttga catgataaaa
     3061 aatctcgata tctttagcgg acttattacc cgttccggaa atatgtacac tgcatcaaat
     3121 cagatacagg aagccaaatt gattattaaa caaacattgg gtgattccga agaggcacag
     3181 gacttacttg caaaacttga caaggaaaag gataaaatag aagactatag aaggagatat
     3241 cctgattaca gacctgtttc cggtagacta acctcaagat ttggttaccg tagagatcct
     3301 tttacaggag aaacaagatt ccacaatgga attgacatag gagccataac aggcacaccg
     3361 atttacagtg cggcttatgg aacagttatc tatacaggat tcaattcctc ttccggcaac
     3421 catataatca tagatcatgg caacggttac aaaacatatt acaagcactt gtctaaaatt
     3481 gaagtcaaaa ccggtaccgt ggtcaacaag ggacaaagaa ttggacaggt tggaagcacg
     3541 ggccgttcaa cagggccgca tctccatttt gagattcatc tttatggaac aataaagaat
     3601 ccacttgaat acgtgtatta caattaagat caaaaaaccc acgcttaatg cgtgggatta
     3661 ttattgtcaa tcaataatgt aaaggatata aagagtaagc aatgaaatag caggaataag
     3721 aattatcctt gcaacagtct taatatacgg tcttgttatt tcccttttat atcgtctata
     3781 tgcaattctg tttagttttg atgacatatt aacaccccgt atatcatact aatattatga
     3841 tatacatttg ggcgaaattg tgtgaatact gtatgtaata agtaattatc tttctgttaa
     3901 ttgctttttt atggtttttt gctataatta tatagcagaa acaagtctgc tattaactcg
     3961 aaagaggatg catgttatgc aaaagaaaag caagaaagta aataaaaatg aaaagaagac
     4021 accgaaagtt gccaaggcat tcttgaagac tttgtcagtg gctataaaga ctacgatagc
     4081 aatggcgctt gtcatagcat gcgtgctggg tggtcttttg attggcgttg ttgcaggttg
     4141 cataattaca accgacactt tggacgaaga agatttatat attaccggct tcacatcata
     4201 tatctatgat gcagatggca atgtaattgc ggaacttaag ggttctgaca acataaacag
     4261 atccgaagtg gatattgagg ttgtaccaaa gcacctgctg aatgccttta tagccataga
     4321 agatgaaagg ttctacaccc atcctggcgt tgacgtgaaa agaacagtag gtgcgttcct
     4381 tggatatttc ataccaggca tgtccagtca tggaggcagt acgataaccc agcagatggt
     4441 caaaaccatt acaggtgatg acgccagaag cgttccaaga aaaatacgcg agcaatggcg
     4501 tgcataccag cttgaaaagg atctcagcaa ggacgagata cttgagctgt atataaacat
     4561 catttacatg ggacacgatc tgtatggtgt taaaaccgct gccaaagcat atttcgaaaa
     4621 agacattacc gagcttaatc tggctgaatg tgcgtttctt gccggaataa caaacaaccc
     4681 aggcaaatac aacccactta ctacactggg ccgcgaaaac gcatacaagc gacaggttat
     4741 tattctcgat cagatgctca agctggattt tatcactgac gaggaatata ttgaagcaat
     4801 tcagacagag cttgtattta atgaagacta cagaaaggag gccagcgcca actccaggca
     4861 ctcctatttt gtagacaccg ttattacaga catcaggaat gacctgattg ccatgggcta
     4921 taccagaaaa caagccacaa atattattta caataccgga gtccatattt acaccactca
     4981 gaatacaaag atacaaaaaa ttgttgagga agaatactgt aagattgaga acttccccat
     5041 caacgaaacc atattggacc cggaagccca ggcccaatct gccattgttg taatggatcc
     5101 aagagctggc catgtcattg ccatttatgg cggatacggt gaaaagaaca agaatctcac
     5161 cttcaacagg gctactagtg catataggca acctggttcc gctataaaac ccttacttat
     5221 atatggacct cagattgata aacatataat tactaccggc acagtccttg aggacaagga
     5281 agtattcctt gacccacaaa ggcctgacgt tccctggccc accaactcag gcggctggca
     5341 cgcaggtctg attacggcaa gaaaagccct tgtaaggtcc tcaaacatat gtgcaatact
     5401 tttgtacaaa gacaatgtta cactggggtt aaactatctg aagaagcttg gcatagacag
     5461 aacagaagaa acccagctgg ctgcaggtct tggcggtttc tcacgaggag ttactccact
     5521 gcagatggct gccgcatatt ccgcctttgc caacagcggc atgtatattg aaccaataac
     5581 atatacaaag gttgtagata gaaacggcaa tgtgattatt aacaagacgc agaaatcgca
     5641 tatagtattt gaagatgaca agaccccata tatcatgaca tcgattctta atcatgtcat
     5701 aaccgactat caaggtaccg gcggtcgtgc acaagtttac aataacaagg gagaaaagat
     5761 tcctaccgca ggcaaaaccg gcacaaccaa caaaaactac gactactggt tctgcggata
     5821 tacaccatac tacacagccg cagtatggta cggatacgac aacttgaagt ctattacctc
     5881 ttccgaatat ggaagtgctg ccagactgtt cggtactgtc atgtcaaaaa tacattcgga
     5941 ccttgaagca attcctttcg aaacctcacc agaccttgtg tatatggaca tatgcaatat
     6001 gtcaggtgga ataccgcaca gcggatgtca tgcggcaaat agaattgttg caaatgaaat
     6061 gttcatacga ggaacagagc ctactgaaga atgcgcttat cacgagccat tagagtaaat
     6121 aaaaacggac gttgacttga atcagcgtcc gttttgtatt attccctaat ctgtcctttt
     6181 ccgttaatta gatatttcac cgtggtaagc tcatacaatc ccataggacc tcttgcatga
     6241 agcttttgat tacttatgcc tatctcggca cccaaaccaa actcaaaacc gtctgtaaat
     6301 cttgtagagc agtttacata gacgctggat gcatccactt cactctggaa tttgcgtgcc
     6361 ttgtcatagg atgttgtaat gattgactca gaatgcatgg taccatgact gttgatatgc
     6421 tcaatagcct cttctatacc agctactgtc ttaatagaca gtacaaggtc attatactct
     6481 gtatcccaat cgctgtcatc agcgtctttt actccttcca tattgccaag tatttctttg
     6541 gatatactgc atactcttac ttcaacattc ttttccataa gttcttttac aaccacaggc
     6601 aggaacttgt ctgcaacggt ttttgaaaca agcagtgtct caatcgcatt gcaaaccgat
     6661 ggtctttgta tcttggcatt catcgtgacg ttcaaagcca tatccaaatc agcgttgcaa
     6721 tccacataaa gatggcatat gccctcacct gtctggatta ctggtactgt ggagttcata
     6781 acaacattgt taatcagacc ttttccacct cttggtataa ggacatcaac atatttattc
     6841 aacttcatta gagcattaac tattgccctg tccgtattat ctatcaggtt tacacaatct
     6901 tcaggaagac cggcatcctt taatgcttcc ctaaaaagct ttgcaatggc aatgtttgag
     6961 ttgacagcct cactgccacc cctcaaaata catgcgttgc cagacttgac acacaaacct
     7021 gcagcatctg cagttacatt tggcctcgac tcaaaaatta ttccaataac gcctagaggc
     7081 actctctttt tgagtatttc aagtccgttc ggcctgacag tgccatggat cacttctcca
     7141 attggatccg gcagattggc aagctgaata agtcctgtag ccatatcatc aattctcttg
     7201 tcggtaagtc tcaacctgtc taccatggct gatgatatgc cgttgttaat tgcatattca
     7261 atatcctttt cattttcctt tataagataa tccctgttct ctttaatctt ttctgcagcc
     7321 agctccaatg ccctgttctt ctttacggta tcggtcactg caagaacacg ggatgcgctt
     7381 tttgcgcgtc taccataatc cttaacatac tcctgaacat tcattatcgc aaacctcctt
     7441 cctaacaaac aaggtaccta aatcctcacc ctccaataac ctgtagatta tctcggggtt
     7501 gtttccattt gcaattattg cattaattcc gctttcagct gcaagtctgc atgcatgtac
     7561 ctttgttagc atgcctcctg ttccgtgctc gccggcacct cctgcagcct cttcaatttt
     7621 ctcagaaaga tccgtgatgt tatgataaag aatcgcatcc gggttctcgt gcggattctt
     7681 gttgtaatat ccatctatat ccgtaaggat aataagaaga tcagctttta taagtttggc
     7741 aaccaaataa gataaattgt cattgtcgcc aaacttaatt tcgtcaatgg caacggtgtc
     7801 attctcattt acaataggca gaatcttcat tttcgccaat tgttcaaatg tatttatagc
     7861 atttgctttc atacatgatc tatcaataac atcctttgtc agcagaatct gagcaacagc
     7921 atgcccatac tcagaaaata tctgactgta cacctgcata aggctggatt gacctactgc
     7981 agcaactgcc tgcttttctg caatggtttt tggtctttcc ttcaacttca acttgcttac
     8041 accaatacct attgcacctg aagacaccag aacaacttcc ttgtcctggt tcatgaggtc
     8101 tgaaattact cttgcaagta tgccaattct tctaaagtta atcctgccat ttgaatgtac
     8161 caaagatgag gtcccaattt ttataacaat tctggttgca ttttttaaac tattccttgc
     8221 ttccctgcaa tcaatattca tctccacacc acctataaaa aggcggaggg acacaagttc
     8281 cctccgtcaa taatcag
//
LOCUS       DAONVY010000025         8273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6565_length_8273_cov_3.597226, whole genome shotgun sequence.
ACCESSION   DAONVY010000025 DAONVY010000000
VERSION     DAONVY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6565_length_8273_cov_3.597226"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLI11_01310"
     CDS             complement(<1..563)
                     /locus_tag="PLI11_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ51532.1"
                     /translation="MLVRFSVENFMSYDKKQTFSMIAGRTRNHPEHLYSVENVELLKV
                     SAIYGANASGKSKLVEAMRFACHVINNEIPVSASNQFCKLKEANKHKPSTFEFEFCVN
                     EMFYAYGFSAILSQRTVVAEWLYRLYPDKDKQECIFERVKDKSGSYSLSLNKLYLNLS
                     DEEANSLQVINDFLDKDRVLFLKTINE"
     gene            complement(787..1062)
                     /locus_tag="PLI11_01315"
     CDS             complement(787..1062)
                     /locus_tag="PLI11_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51533.1"
                     /translation="MFYNIPGGSATGNQVIPGTYEDTTDWQLILLDLVSGFGSIGYFR
                     YDTFTSSKACDVLEIESIRIFKTKEDALKAYKNKYCKKKIYICMTVP"
     gene            complement(1069..1692)
                     /locus_tag="PLI11_01320"
     CDS             complement(1069..1692)
                     /locus_tag="PLI11_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51534.1"
                     /translation="MRKSSRSQILCGYLIDSPNPQNVKVVGAVAVRFTVPDGYELEEF
                     VFLECPTWDQTDSCVDIHLYDTTVEGTIFGTYHAGEYLVVMDASSATNSIGPWTNKDI
                     DGQETYVNGALLDGFDARTAIIISENVDTNDFPAESTVKLDFKNPYTMSKVSAGVGTA
                     VSYKNGVLKVTHQDSNDPDIHINFDPVNSDIYKCAVVKVRRSATEIR"
     gene            complement(1870..2133)
                     /locus_tag="PLI11_01325"
     CDS             complement(1870..2133)
                     /locus_tag="PLI11_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51535.1"
                     /translation="MKELFVALMCFVVLFAMSFELMNVFADTGSGSLVPINDTMSKVE
                     VYSQESSSSRPKSLTIIQIILLVIFVLMLSSVIIIMIKRSKKK"
     gene            complement(2225..3262)
                     /locus_tag="PLI11_01330"
     CDS             complement(2225..3262)
                     /locus_tag="PLI11_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51536.1"
                     /translation="MRGDNFQEQWDVAIGLDPEIVFITGWNEWTALKLTADWAGSTPF
                     WVDTFNTEFSRDIEMTKYPTYVVGENGEYIQEGYGDNFYLQMISNIRRFKGIPLSEGN
                     RYVHVSKTIDITGDLSQWDDIKNRYLNLSTDKIERDHYGYVNVKAYHYTQPAPKNFIT
                     DIKVTHDKENIYLLVNTKGKITDFDANSTNWMNLFIGVKGSKASSWEGFQYVVNRKPV
                     SNTKTTLEKVAENGKHVFTESSRIDYVVKDNTMQIKIPRKDIGIKKDDFTLYFKVADS
                     IEKEDDIMDYYVSGDSVPMGRLVFTYSNGTEGKALKLSMLELVLLLASILLIGACAIV
                     FASHLARRKKG"
     gene            complement(3259..4176)
                     /locus_tag="PLI11_01335"
     CDS             complement(3259..4176)
                     /locus_tag="PLI11_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51537.1"
                     /translation="MKRWSVVLLLIMAVNMTFVFAEGMGGNNMSDHDFTLMNIAATDA
                     LGRKLPEVAGFRKDKYVGIFYFTWLGQQAEPQTEVYDITKLLREDMESLFDIRSRKAP
                     SGFNYFFNEPLFGYYNSADPWVIRKHIEMFVAAGIDFLALDFTNAIVYTKVLPVLLDL
                     LLEYQEAGWKVPQVTFFTNTQSGYVVRTLYNSVYKEEKYASLWFRGNKGKPYIIAWEN
                     ELTNEMKEFFHVRPPQWPEAEYNELGFPYVEKVRPQRVFTDLVSVSVAQHTGDAFSFS
                     LRGVDGGKKESWGRGYTSKNPRNGDVEAS"
     gene            4370..5332
                     /locus_tag="PLI11_01340"
     CDS             4370..5332
                     /locus_tag="PLI11_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPZ51538.1"
                     /translation="MDKLTLSRIAKMHKTSTTTVYNALYSRTPVNTKLKKEILNTAES
                     IGYFAQNKVPVCDVAVILPSNPVYFWSEAEKGILNEIRNSNISIKLFTYSNMDDEEDF
                     VLCMESAKRSNSKVYIITPVNTEKTIRIFNELDKNIVFLNEYVKIDKAGFVGADAYKD
                     GQMLAQACYDFLKKHPRILIINATAKNCKAEERKLGFCESLPESARITGEIKLPFNRT
                     LAASIAREIEIRYQGKFDCIFVAQGWFQQVSMALKKLDLVIPCIGCENQKVSPKYAEN
                     GKLHAALVQHIRKQGEQSIKYAMKLIEGVELPGRNITVESTLMI"
     gene            5347..>8273
                     /locus_tag="PLI11_01345"
     CDS             5347..>8273
                     /locus_tag="PLI11_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HPZ51539.1"
                     /translation="MTRKATLSVLAAVLLLLCITSLKAASSFDNWDLGSWTVKTIDGE
                     SVVCAPDNNYHSHIETRQASDKNQIRLTAKVDSSTATIDGNFGVLFKCNDGTLYFFEY
                     NIVYDILRVRRLGTDGSDHHLASPVPFSIPIGQWFDYEITVSNTNIQWYINGDLKYDI
                     DLNTNELSQGKFRVQGYFCTPFVKNIELRKVENMKKTLDLTFSTPESIDFFKVNNGSL
                     SYENGALKYTFNESSSLVSEQIDVNAGHKYSALLRLRNTILMRIKNASSASKVKISFI
                     TSEDMTYDEAKSKVFDLKPGNDYLPYYFNLSDLPGAKGYLKGFKIEPIGGTGYLLIDT
                     IQFEREKPLIDYAGTITSCIANEKDIVIKGTLKPEYANKKVYIYETNIINYNNSLNGL
                     KVVGETVSQGCDFTVKIPFKNGNMTRLSSHFLAVVEDKLISDKFNIENYRDFTDNPYE
                     FELPDRVVHVTDAPFNAKGDHFTDDTDAIQAAIDYISSQGGGRVVIPGDLTTPEGRRY
                     VVTCIKLKSNVELHIEKGATLWQSQNEDDYKYEVAYGHDVSIPGVNWTHAGLCHNYPL
                     ILVSGCENVKITGGGTLRGMDPGSECEDGTNPNTLWIGCPNKIHVILLGMYDSKNVEI
                     SDLELIRSNVYHISTYACENVYFGNVTMHEVTCASGDGIGIGIGSKNIVVDRCFLRSN
                     DDGVVLCSSYNEPRGLVWWFPKPDADNCISNIRLSHSCIFGGHGLTFIPWGTDNPDLS
                     KQEISDVVAYDNILGGGYSVGTWCDNPYYGEQPFTNTEEDDYSPVKNVRIYDNLYLAK
                     ADLLTIKATNIITDCGLNSASDFQHGDFERKNGQSNWIAGLSNWSYEKNSSPNSVGVT
                     KNNGGYCGYISDLDKGNARLYQGLYLTRGKHTINVDLKTSGVTARFFVQNVRTNEIVK
                     SVEFSNTEFKTYEIVFETQRSDTYYIGIDAGSAKSGSAYIDNAKVTTETLPVPKYFRT
                     NMD"
BASE COUNT         2625 a         1746 c         1544 g         2358 t
ORIGIN      
        1 ctctcattta tggtcttcaa gaacaatact ctgtctttat ctaaaaagtc attgatgact
       61 tgtagcgaat ttgcttcctc atccgatagg ttcaagtaga gtttgttcag actcaaacta
      121 tatgaacctg acttgtcttt cactctctca aaaatgcact cctgtttgtc tttatctgga
      181 tatagtctgt aaagccactc agcaacaact gtgcgctgtg acaaaatggc agaaaaaccg
      241 tatgcataaa acatttcatt aacacaaaac tcaaactcaa aagttgacgg cttgtgttta
      301 ttcgcttctt ttagcttgca aaactggttg gaagcactaa ctggaatctc attattaatc
      361 acatgacaag caaacctcat tgcttcaacc aacttgctct ttcctgatgc attggcacca
      421 taaatggcac ttactttaag taattcaaca ttttcaacac tatataaatg ttcaggatga
      481 tttcttgttc tgccggcaat catcgagaaa gtttgcttct tatcatatga cataaaattc
      541 tctacagaaa atctaacaag catggtttca ccccttaatg agaaattttc tctgtttata
      601 tatattatta ccacaatatt tgttttaaca caagattatt cttgttttaa tagagatttt
      661 ttcgtattta tgcaaccttc tctttatatt tctacttctt taaaaattgg caaaactaat
      721 tgatgataaa gaaggagagc actcagacgc cttctccttc tttttacgta cctactctgt
      781 cgtttatcaa ggtactgtca tgcaaatata aattttcttt ttacaatatt tatttttgta
      841 tgcttttaat gcatcctctt ttgtcttgaa gatgcggatg gattcaatct ccagaacatc
      901 acaagccttg cttgacgtaa aagtatcata gcggaaatac cctatagaac caaatccaga
      961 cacgagatcc aaaagaatca gctgccaatc tgtagtatct tcatatgtgc ctggtatgac
     1021 ttgattgcct gtggcactac ctccaggaat gttataaaac atgtcaaatt atctgatttc
     1081 agtagcactc cttctgactt ttacaaccgc acatttgtat atatcactgt taaccggatc
     1141 aaaatttata tgtatgtctg gatcgtttga atcctgatgg gtaactttaa gaaccccgtt
     1201 tttataagat acggctgtac ctactcctgc agaaaccttg ctcattgtat aagggttttt
     1261 aaagtccagc ttgacagttg actctgctgg aaaatcattg gtatcaacat tctctgaaat
     1321 aattatggcg gttcttgcat caaatccgtc aagcaaagcg ccatttacat aagtttcctg
     1381 gccatcaata tccttgtttg tccatggtcc tattgagttt gttgcagaac ttgcatccat
     1441 aacaacaagg tactcacccg catgatatgt accaaaaatt gtaccttcga ctgtagtgtc
     1501 gtacaaatgt atatcgacac agctatccgt ctgatcccag gttggacact caagaaatac
     1561 aaactcctca agttcatatc cgtcaggaac agtaaacctt actgctacag ccccaaccac
     1621 ctttacattt tgaggatttg gagaatcaat cagataacca cacagtattt gtgaccttga
     1681 tgatttccgc acttgccgga atcattaaag ataaagcaca agcctataca agcatgaaaa
     1741 cgatcaaacg ctgttgcatt gtgccgataa tattacacgg ataatgtaaa gtcaggaaat
     1801 aaatcaatac atttagaata agcaggagtg gatttcgaag agtgcatcat tatacttgca
     1861 ctgactgttt tacttctttt tgctcctctt tatcatgata ataataacgg aactgagcat
     1921 cagtacaaag atgacaagga gtattatttg aattatggta agcgatttag gtcgtgaaga
     1981 cgaactctcc tgtgaataaa cctccacttt gctcatggtg tcgtttattg gcacaagact
     2041 gccactgcct gtatctgcaa atacattcat gagctcaaaa gacatagcga ataatactac
     2101 aaaacacatt agcgccacaa ataattcttt catttcaacc ccctcttgct accattgaaa
     2161 ttacgtattt tgtcttgaaa actaaaacat actataatct gttttaccaa aacttacaat
     2221 acttctatcc ctttttcctt cttgccaagt gactggcaaa cactattgcg catgcaccaa
     2281 tcaaaagtat tgatgccaaa agcaatacca gttcaagcat agaaagcttt aaggcttttc
     2341 cttcagtacc attactgtat gtaaacacca gcctgcccat gggcactgag tcacccgaca
     2401 cataataatc cattatgtcg tcttcctttt caatactgtc cgccaccttg aaatacaagg
     2461 taaaatcatc tttctttata ccaatatctt ttcttggtat cttaatctgc atggtgttat
     2521 cttttacaac ataatctatc cgggacgatt cggtaaaaac atgcttgcca ttctccgcta
     2581 ctttttccaa tgtggttttg gtattggata caggctttct gtttactaca tattgaaaac
     2641 cttcccagga agatgccttg cttcctttta cacctataaa cagattcatc cagttggttg
     2701 agttggcatc aaaatccgtt attttgccct tggtgtttac aagtaagtag atgttttcct
     2761 tgtcatgggt taccttgatg tcagttatga agtttttagg tgcaggttgt gtataatggt
     2821 acgcctttac atttacataa ccgtaatggt ccctttctat tttatctgta gacagattca
     2881 aatacctgtt cttgatatca tcccactggg acaaatcacc agttatatct attgtctttg
     2941 aaacatgcac atacctatta ccttcactta gtggtatgcc tttgaatctt cggatgtttg
     3001 aaatcatctg aagataaaag ttgtctccat atccttcctg tatgtattcc ccgttctctc
     3061 caacaacata tgttggatat ttcgtcattt caatatctct ggaaaactca gtattgaaag
     3121 tgtccaccca gaaaggagtt gaacccgccc agtccgcagt cagtttcaaa gcagtccatt
     3181 cgttccagcc cgttataaac acgatctcag gatccaaccc tattgccaca tcccactgct
     3241 cctggaagtt gtctcctctc aagatgcctc cacatctcca tttcttggat tcttggaggt
     3301 atatcccctt ccccagcttt ctttttttcc accgtcaacg cctcttaagg aaaagctgaa
     3361 ggcatcgcct gtatgctgtg ccaccgacac actgacaaga tcggtaaaca ctctttgagg
     3421 acgcaccttc tccacatacg gaaaaccaag ctcattgtat tcagcctccg gccattgggg
     3481 cggcctgaca tggaaaaact ctttcatttc atttgtcaat tcgttttccc atgcaattat
     3541 gtaaggtttt cctttgtttc ctctaaacca caaactggcg tatttctcct ctttgtatac
     3601 actgttgtac agagtcctta caacataacc tgactgggtg tttgtaaaga aggttacctg
     3661 cggaactttc cagccagcct cttgatactc aagaagcaag tccagtaaaa caggcaaaac
     3721 tttagtgtac actatcgcat ttgtgaaatc gagggccaga aaatctatgc ctgctgcaac
     3781 aaacatttct atatgtttgc ggattaccca agggtctgca ctgttgtagt atccaaaaag
     3841 aggttcattg aaaaagtagt tgaatccaga aggagctttt ctgcttctta tgtcaaacag
     3901 actttccatg tcctctctaa gaagcttggt tatgtcatat acttcagtct ggggttctgc
     3961 ctgctgacca agccatgtaa agtagaagat gcctacatat ttgtcttttc tgaatccagc
     4021 aacctccggc agttttctgc ccaaggcatc cgttgcagca atattcatca aagtaaagtc
     4081 atggtcgctc atattattcc cacccattcc ctctgcaaac acaaaagtca tattaacagc
     4141 cattattaaa agcaaaacaa cagaccatct tttcatgcaa atccctctgt acttgttatt
     4201 ccaatacact ttactctaat aaattgaaat ttacaacatt ttcacggcaa gagttttcgc
     4261 ttttatgcaa acttaacaaa acacatactc cacttttgtt aaccctacac attatattta
     4321 aaatccccta cctatataat aaacacagtg cacaagcgag gtgaacacaa tggacaaatt
     4381 gacattaagc aggattgcca aaatgcataa aacatccacc acaactgtat acaatgccct
     4441 atacagccgt acacctgtca acacaaaact gaaaaaagaa atacttaaca ctgctgaatc
     4501 gattggatat tttgcacaaa acaaagtgcc cgtttgtgat gtggctgtga ttttgccatc
     4561 caaccctgtt tacttttggt ccgaagctga aaaaggaata ctgaatgaaa taaggaacag
     4621 caatatttca attaaattgt ttacctacag caacatggat gacgaggaag actttgtgtt
     4681 gtgtatggaa agtgccaaga ggtcgaattc caaagtctac ataatcaccc ctgtaaacac
     4741 agaaaagaca ataaggattt ttaatgaact tgacaagaac atagtttttc taaacgagta
     4801 tgtgaaaata gacaaagcag gctttgttgg agcagatgca tataaagacg ggcaaatgct
     4861 ggcacaagcc tgctatgatt ttctcaaaaa gcatcccaga atattgataa taaatgcaac
     4921 agcaaagaat tgcaaagccg aggaaagaaa gcttggcttc tgcgagtctt tgcctgaaag
     4981 tgctcggata acaggtgaaa taaagctgcc atttaacaga acccttgccg ccagtattgc
     5041 aagagaaata gaaatcagat atcaaggcaa attcgactgt atttttgttg cccaaggatg
     5101 gtttcaacaa gtgtcaatgg cacttaagaa actggacttg gtaataccat gtatcggatg
     5161 tgaaaaccaa aaagtaagtc ccaaatacgc tgaaaacgga aaactccatg cagcactggt
     5221 gcagcacata aggaagcagg gcgagcaaag catcaagtat gcaatgaaac ttatagaagg
     5281 agttgagttg cctggtcgga acataacagt tgaatcaact ttaatgatat aaggggtgtg
     5341 tataaaatga caaggaaagc cacattaagt gttctggcag cagttttgct gctattatgt
     5401 ataaccagtc tgaaagctgc ttcaagcttt gataactggg atttaggttc ctggactgtc
     5461 aagaccattg atggggaaag cgtggtctgt gctccggaca acaactatca cagccacatt
     5521 gaaaccagac aggcatctga caagaaccag atacgactga ctgccaaagt tgacagcagt
     5581 actgcaacaa tagatggcaa ctttggtgtg ttgttcaaat gcaacgatgg aactttgtac
     5641 ttctttgagt acaatattgt atatgacata ctaagggtaa gaagactggg aacagacgga
     5701 agcgaccatc atcttgcaag cccggtgcct ttttctattc ctattggaca gtggtttgac
     5761 tatgaaataa ctgtcagcaa tacaaatata cagtggtaca taaacggaga tttgaaatat
     5821 gatatagatt taaataccaa tgagctgtcg caaggaaagt ttagggtaca agggtacttc
     5881 tgtactccat ttgttaagaa tatcgaatta agaaaggttg agaatatgaa gaaaacactg
     5941 gatttaacct tcagtacgcc tgaaagcatt gacttcttta aagtaaacaa cggaagctta
     6001 tcctatgaga acggagcatt gaaatacact tttaatgagt cctcatctct tgtatcggaa
     6061 cagatagatg tcaatgcagg acacaaatac agtgcacttt tgagactcag aaacactata
     6121 ctgatgagaa ttaagaatgc aagcagtgcc agcaaggtga aaataagctt tatcacatca
     6181 gaggacatga cgtacgatga agcaaaatca aaggtatttg acctaaaacc agggaatgat
     6241 tatctgcctt actacttcaa tctatccgac ctgcctggag caaaaggtta tcttaaagga
     6301 ttcaagattg agcccattgg cggaacaggc tacctcctta tagacacaat acagtttgag
     6361 agagaaaaac ctttaattga ttacgcaggt acaattacaa gctgcattgc aaatgagaag
     6421 gacatagtta tcaaaggtac actaaaacct gaatacgcaa acaagaaagt ctacatttac
     6481 gaaacaaata taataaacta caacaacagc ctaaatggtc tcaaggttgt tggtgaaaca
     6541 gtttcacaag gatgtgactt tactgtcaag attccgttca agaacggaaa catgacccgc
     6601 ttgtcctcgc acttcctggc tgtagttgag gataaactca taagcgataa attcaatatt
     6661 gaaaactaca gagactttac tgacaatcca tacgaatttg aacttcctga cagggttgtc
     6721 catgtaacag atgctccctt caatgcaaaa ggagaccatt ttacagatga caccgatgcg
     6781 attcaggcag caatagacta cataagcagc cagggtggag gtcgtgtggt tatccccggc
     6841 gacttgacaa cacctgaagg aagaaggtat gtggtcacct gtataaagct aaagagcaat
     6901 gtggagcttc atattgaaaa gggagccact ttgtggcagt cacaaaatga agacgactac
     6961 aagtacgagg ttgcatacgg tcatgatgtg tccatcccag gagtcaactg gacacatgca
     7021 ggactttgcc acaactaccc attgatactt gtatccggtt gtgaaaatgt aaagattact
     7081 ggcggcggta cgctaagagg aatggaccca ggcagtgaat gcgaggatgg taccaatcca
     7141 aatactttat ggataggctg cccgaacaag atacatgtaa tacttttagg catgtacgac
     7201 agcaagaatg ttgaaatatc ggatctggaa ctaatccgct caaatgtata ccacatatca
     7261 acttacgcat gtgaaaacgt atactttgga aatgtgacaa tgcacgaagt cacctgcgca
     7321 agtggagacg gcataggtat cggcattggt tcaaaaaaca ttgtagtaga cagatgcttc
     7381 ctaagatcca acgatgacgg cgtcgttctt tgctcttcat acaatgagcc cagaggcctt
     7441 gtatggtggt tcccaaaacc agacgcagac aactgcataa gcaacataag attgtcacac
     7501 agctgcatct ttggcggaca tggtttgaca ttcataccct ggggtactga caatccggat
     7561 ttgtcaaaac aggaaatctc agacgttgta gcttacgaca acatcctagg cggcggttac
     7621 tcagtaggta catggtgcga caacccatat tacggtgaac aaccctttac caatacggaa
     7681 gaggatgact actcacctgt aaagaatgta agaatctatg acaatttgta tcttgccaaa
     7741 gctgatcttc tgaccataaa agcaacaaac ataattacgg attgcggtct taacagtgca
     7801 tcggatttcc aacatggaga ctttgagaga aagaatggac aaagcaactg gattgccggt
     7861 ctttccaact ggtcatatga gaaaaattca tcaccaaact ctgtcggagt aacgaaaaac
     7921 aacggcggct attgtggata cataagcgac cttgacaaag gtaatgcaag gctctaccag
     7981 ggactctacc tgacaagagg caagcacaca ataaatgttg acctgaaaac cagcggagtt
     8041 acagcaaggt tctttgtgca gaatgtaagg acgaatgaga ttgtaaagag tgttgagttt
     8101 tcgaatactg agttcaagac ttatgaaata gtatttgaga cacaaagatc agatacctat
     8161 tatattggta ttgatgcagg cagcgcaaag agcggctcag cttacataga caacgcgaaa
     8221 gttaccaccg aaacccttcc tgttccaaaa tattttagaa caaacatgga tga
//
LOCUS       DAONVY010000026         8131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6721_length_8131_cov_3.959757, whole genome shotgun sequence.
ACCESSION   DAONVY010000026 DAONVY010000000
VERSION     DAONVY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_8131_cov_3.959757"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLI11_01350"
     CDS             <1..448
                     /locus_tag="PLI11_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ51540.1"
                     /translation="PADGFFLRAESFYNVATEIDKLDEEYPYPLLESYGGKSLHGQSH
                     GESFISLVQNRFFGNGLYILDEPEAALSPVRQMSLLVYIHELVKLNSQFIISTHSPIL
                     LSYPNADIYELTENDIRLTHYKDTEHYVLTKQFLNNPEGMLKYLLD"
     gene            521..1942
                     /locus_tag="PLI11_01355"
     CDS             521..1942
                     /locus_tag="PLI11_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPZ51541.1"
                     /translation="MNFNGLKSNVVYFRGLKRHNIPYVLVWVIFYAWIVAFASWWTAS
                     PNVKNVFGSVDRILIQSALLTSSLVFMFFIKKKWFVKVSRIIAAFSLFGLALFFLATS
                     VIPRVIGLVVFGISLGCVSLLVFIPMVFRLNNTEKLYASIISNICVSILLIIQNMHNG
                     KILPPASDLILFLPVVIIAIATIIFINRKTVVLYEEGRDDNIPKVGFNIYSTLFFNAS
                     FVIICIGIGVGVLNTSIKKFGSNVAVWYFVGGVIGCVIFYTIYALFPKAILIVGNISF
                     GFVSLGFLCSAFGAQASEVIVLFALLLGIGNGAGMIVMFYVIGIVGKKINSLKYLKLA
                     ILFVGIIGGVGGMVLGNLLQYVNSTQVSMFASIISALFLITFMIMSPLLAQEHIYDGW
                     AKDTEIIDIDNDKMYLFEKYQLSKREIEVCRLLLKGHTLRQISAMLSISYSTVNTYCT
                     NCYRKLNINSRTELLILFKDYIE"
     gene            2125..2853
                     /locus_tag="PLI11_01360"
     CDS             2125..2853
                     /locus_tag="PLI11_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51542.1"
                     /translation="MRLSKIILFILLSIVLALLVTSCFWDGSIKLGDKDGEIQIGKTK
                     WDKKEMFGLDAPKAKLTSSVSTEEGAMYFFSEMKEKDAEAYIEMLQEKGFTFNKLVFE
                     EYSYTGTNKDGETISFWYDKESGDGTITASKGEKPTGNEDDEVIIGGNRKWDSSKMAG
                     LPDPGTKVVNYWSINKQTAYTLEPLADYLDYVEEIKACGFVVDIQTAEINDIYIFSAS
                     NENGDKVEFAASPESLSVVVNLAE"
     gene            2882..3310
                     /locus_tag="PLI11_01365"
     CDS             2882..3310
                     /locus_tag="PLI11_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPZ51543.1"
                     /translation="MYCSNCGAENNEEAKFCNKCGSTVAGNTPAVKEVEDNKVIFILA
                     YLGILFFLPLVACPNSKVGRFHANQGLLLFILSVAGQIVLGILSLIAGAIYWLFGAFI
                     SFISWIWWILILVLAIMCMISANKGEQKPLPVIGKIKIIK"
     gene            3323..3853
                     /locus_tag="PLI11_01370"
     CDS             3323..3853
                     /locus_tag="PLI11_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51544.1"
                     /translation="MFKKFVIWCVLIFVAFSLTACISNIDDNNGENKTPEKTETNQTD
                     ATPTPASTDKKGDPVPTYNEDWPTEMLPDGFPNLGKVTKVSDKRSYGNVITIYWNMIT
                     EDQIDAFVDKLNDYLDNDHAWQGSFYSDGVKYKPGTEDEFIRVVIRHMDFASGEVDSD
                     FEPQFYLEISGEGLKK"
     gene            complement(3924..5630)
                     /locus_tag="PLI11_01375"
     CDS             complement(3924..5630)
                     /locus_tag="PLI11_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51545.1"
                     /translation="MLEKKYIDHFVDGEYVYLLMSGSTKDNVIWVFTNGTFETASTFE
                     APESSKLLYVEGDTLYIGTFDTIYEYDKNTYEQKKKHTINVMTNNMLVYNGIIYISAV
                     RTGGQHIIAIPPEGPGFSARVDNTEGMCVNKDEGILYVAGKSKLYAFKLDNLELIKEI
                     DLPHNQEKKMVFYDSGKVYTAGCVYDSSNLDNFIALIEINPAKEKETYYADSNYVITE
                     EGVFDSQTLDIIMPLERKLHRCFITSSGNVFIIPSQSGTIIDIQNINDYLDDIKSKTF
                     YTIVEKDASLAWNGKPKITSYLLNDEVIDMAGTDKICYLATSNQNKLIAFKVDTMEVV
                     FSFLLPGVPGRLVIYDSEAMISFPDLKVIKCYDRTTGKYLRDIPLENQVYSFAVHGSF
                     LYYTIKGSSYIYKHNLGDNTSISKSVSSDLDFVINAEDEVIYAANDRNLYVLDMHELN
                     IFSIFSSDSYAGKSTRTVFYIDYKIYALGHAFDKNDTTKPIMNYSTSKSDMKVLFADN
                     EYVVCIDGIYSRKSGERLITIDLNLLNAWIEKGYFVGFVKGYTKSGSKLYVGSMSSMV
                     NG"
     gene            5990..6292
                     /locus_tag="PLI11_01380"
     CDS             5990..6292
                     /locus_tag="PLI11_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HPZ51546.1"
                     /translation="MIDVKPINLDGYVFIAVSVQLPKTTLLVVSSDKGYIMCGALDVS
                     LLNEKLKDRKIIAGRAVGVRTIEELLEAPLESVTNEAEKLGITKGMPGKKALLKMV"
     gene            6346..6540
                     /locus_tag="PLI11_01385"
     CDS             6346..6540
                     /locus_tag="PLI11_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51547.1"
                     /translation="MEKNLTNGEVPIGFAMALAQNMNSMQYFSSLSPDKQRSVIGRTR
                     GIKSKAEMQAFVDSISSIGI"
     gene            complement(6945..>8131)
                     /locus_tag="PLI11_01390"
     CDS             complement(6945..>8131)
                     /locus_tag="PLI11_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPZ51548.1"
                     /translation="TVFLADYPPEYSYFDNRFTFELQNIVSQKTVLALEKFSYGTAYE
                     LLKQGGNVNYSPLSLYFALSLCAQGAEGETFEELMNVLGLNNMSKEFLMDETRKLYNR
                     LYIDHEFGKLKIANSVWLSNLNGVTYKQTFIDNARQHFYATLFSVDFTDEDTSKLMKK
                     WIFDNTEGTLAPDFPHDPTTIFKIINTIYYRNQWNEEFEEENNYLDKFYGCTETTDME
                     FMKRTDVQAEYYKGEDYACTTLRVKGGKMVFLLPDEGVSIEDFLTSPGKLEAAIKSPT
                     IYAPLFDGYMVTLHLPKFGFESKLDLLDMVKNLGLNDAFTDVADFSAITDIDLFLTDI
                     KQDTYIQVDEKGITASAVTNISGGMGSPEWMEVEVNLNRPFIYCIMHEEKMPIFLGVF
                     AN"
BASE COUNT         2322 a         1441 c         1672 g         2696 t
ORIGIN      
        1 acctgcggac ggttttttcc tacgagccga aagcttttac aatgtagcaa ctgagattga
       61 caaactcgac gaagaatacc cttatccatt gctggagtcc tatggaggca aatccttgca
      121 tggacaatcc catggtgaaa gttttatatc tctcgttcaa aatcgcttct ttggtaatgg
      181 tctgtacata ctggatgagc cggaggcggc tctgtcacca gtaaggcaaa tgagtctttt
      241 ggtttacatc catgaactgg tgaagttaaa ctctcagttt attatctcta cgcattctcc
      301 tattttgctt tcatacccta atgcggatat ttacgagcta actgaaaacg acatcaggct
      361 tactcattac aaggatacag aacattatgt tctgacaaaa cagtttttga acaatccgga
      421 aggcatgctc aaataccttc ttgattgagt ttatgttttt tagtcgaaat ttattgattt
      481 gaagagcata agattatgga gaaaaagagg tgctcttatt atgaacttta atggattaaa
      541 gtccaatgtc gtttatttca gagggttgaa aaggcataac atcccttatg tcttggtgtg
      601 ggttatattt tacgcatgga ttgttgcctt tgcttcttgg tggacagcat ctcccaatgt
      661 gaaaaatgtc tttggttctg ttgacagaat cctcattcaa tccgctttat tgacatcttc
      721 tcttgttttc atgtttttta taaaaaaaaa gtggtttgta aaagtgtcac gaatcattgc
      781 ggccttttcc ctttttggtt tggcactatt cttccttgca acatcagtta ttccccgggt
      841 tattggcctt gttgtatttg gaatttcgtt ggggtgtgtc agcctgttag tgtttatacc
      901 tatggtcttt cgtttgaata atactgaaaa gctttatgcc agcattatct caaatatatg
      961 tgtgtccata cttttgataa ttcagaacat gcacaatggg aagatactcc ctcctgcgag
     1021 tgatctgatt ttgttcctcc cggttgttat tattgctata gcaacaataa ttttcataaa
     1081 caggaaaaca gttgtgttat atgaagaggg aagggatgac aacattccga aagttggatt
     1141 taatatttat tcaacactgt ttttcaacgc ttcctttgta attatatgca taggtatcgg
     1201 agttggggtc ctgaatactt ccataaaaaa gtttggaagc aatgttgcgg tttggtattt
     1261 tgtaggaggg gtgattggct gcgttatctt ttatactata tatgcattgt ttccaaaagc
     1321 aatccttatt gtagggaaca tatcttttgg atttgtttct cttggctttc tctgcagtgc
     1381 ctttggtgcc caagcttccg aagttattgt cttgtttgcc ttgctccttg gaatagggaa
     1441 tggggcaggc atgattgtaa tgttttatgt tattgggatt gttggaaaga agattaacag
     1501 cttgaaatat ttgaaactag ccatcctttt tgtaggaatt ataggtggtg ttggaggcat
     1561 ggttctgggg aatcttcttc agtatgttaa cagcacccag gtctcaatgt ttgcgtcaat
     1621 catatccgca ttgtttttaa taacctttat gataatgtca ccattgcttg cacaagaaca
     1681 tatctatgat ggctgggcaa aggatacgga gataatagat attgataatg acaaaatgta
     1741 tttgtttgaa aaatatcagt taagcaaaag agaaattgaa gtgtgcagac ttttgctgaa
     1801 aggacataca cttaggcaga tttcagccat gctttccata tcctactcga cggttaatac
     1861 gtattgcaca aactgttaca gaaagttgaa tatcaacagc aggacggaat tgttaattct
     1921 ttttaaggac tacatagaat aaatgcaatt atatttgtca aaaaagcgcc tcattagaac
     1981 taatgaggtg ctttttttat gccttcatgt cttttaacta attgccataa tcgctcttaa
     2041 atcataagct gattaaggaa acgtggccaa tgttcgaaaa tggtatgttt gccatgtaaa
     2101 actaataaag aatggagtgt ttgcatgaga ttgtcaaaaa tcattttatt tattctgctg
     2161 agcatagtcc ttgctctttt agttaccagc tgtttttggg acggtagtat caaactaggg
     2221 gataaggatg gagaaattca aatcggaaag acaaaatggg acaaaaagga aatgtttggt
     2281 cttgatgcgc cgaaagcaaa actgaccagc agtgtcagca ctgaagaagg agcaatgtac
     2341 tttttctctg aaatgaaaga aaaggatgcg gaagcataca tagaaatgct tcaggagaaa
     2401 ggatttacat tcaacaaact tgtgtttgaa gagtacagtt acactggcac caacaaggat
     2461 ggagaaacaa tatcgttttg gtatgacaag gaaagtggtg acggcaccat tactgcaagc
     2521 aagggtgaaa agcctaccgg taatgaggat gatgaagtaa ttattggtgg taatagaaag
     2581 tgggatagta gcaagatggc cggactgcct gatcctggta caaaagttgt aaactactgg
     2641 tccattaata aacaaacagc ttatacactg gaacctttgg cggattattt ggattacgtg
     2701 gaagaaatca aggcgtgcgg atttgtagta gacattcaaa ctgctgaaat caatgatata
     2761 tacatatttt cggcgagcaa cgagaatggt gataaggttg agtttgcagc atcgcctgag
     2821 tccttgtccg ttgttgtaaa tcttgcagaa tgaatgataa aagtgtgaga agggggatat
     2881 tatgtactgt tcgaactgtg gagcggaaaa taatgaggag gccaagtttt gtaacaaatg
     2941 cggctcaact gtagctggaa atacgccagc tgtaaaagaa gtggaagaca acaaagtaat
     3001 atttatactg gcatatctgg gcatattgtt tttcctgcca ttggtcgcct gtccaaactc
     3061 taaagttggc aggtttcatg caaaccaagg tttactgcta tttattttgt cagttgcagg
     3121 gcagattgtt ctaggtatat tgtcgcttat agctggtgct atttattggt tatttggagc
     3181 ttttatatct tttatttcct ggatatggtg gatactgatt cttgtgcttg ccattatgtg
     3241 catgataagt gcaaataagg gggaacagaa gcctcttccc gttattggca agataaaaat
     3301 aattaaataa ggagttgatt gaatgtttaa aaaatttgtc atatggtgcg ttctcatttt
     3361 tgtggctttt tctttgacgg cttgtatatc taatattgat gacaataacg gtgaaaacaa
     3421 gacacctgaa aaaacagaaa cgaatcaaac ggatgcaacg cctactcctg cttctacaga
     3481 caagaaggga gatccggttc caacatataa tgaggattgg ccaactgaaa tgttgccgga
     3541 tggttttccg aatctgggga aagttacaaa agtgagcgac aaaaggtcct atggcaacgt
     3601 tataactatt tactggaaca tgataactga ggaccaaatt gatgcatttg ttgataaact
     3661 aaatgattat cttgataatg accatgcatg gcaaggttct ttttacagtg acggtgtaaa
     3721 gtataaaccg ggaaccgaag atgaatttat cagagttgtt ataaggcata tggattttgc
     3781 ttcaggagag gttgattcag attttgagcc tcagttttac ctggagatta gcggtgaagg
     3841 cttgaaaaag tgaatacctt tgataaaaag cttactcata actatgcaag gcggcaagtt
     3901 gtatgaactt gccgtcttgt attctatcca tttaccattg aagacatgga gccaacataa
     3961 agtttgcttc cggattttgt gtatcctttt acaaagccta caaagtatcc cttttcgatc
     4021 caggcattta gcaaattcag gtctatcgtt attagccgct cgcctgattt tcttgagtag
     4081 atgccgtcaa tgcatacaac atattcatta tctgcgaaaa gtaccttcat gtcgcttttt
     4141 gatgtactat aattcattat tggttttgtt gtgtcgtttt tatcaaaagc atggcctaat
     4201 gcatatatct tgtagtctat atagaatacg gttcttgtgc ttttgccagc atagctgtca
     4261 ctactgaata ttgagaaaat attaagttca tgcatatcca gaacgtacag attacgatca
     4321 tttgctgcat atattacttc atcctccgca tttataacga aatccaaatc agaactgacg
     4381 gatttggaaa ttgatgtgtt gtcaccaagg ttgtgtttgt agatgtatga tgagccttta
     4441 atggtatagt aaagaaagct gccatgtaca gcaaacgagt atacctggtt ttcaaggggt
     4501 atatccctta agtacttgcc cgttgtccta tcatagcatt ttataacttt tagatcaggg
     4561 aaggaaatca ttgcctcact atcatatata accaacctgc cgggaacgcc aggcagcagg
     4621 aaagagaaaa caacttccat ggtatccacc ttgaaggcaa tcagcttgtt ctgattggat
     4681 gttgccaagt agcatatttt gtctgttcct gccatgtcaa tcacttcatc attcagcaag
     4741 tagcttgtaa ttttgggctt tccattccag gcaagggatg catccttttc tacaattgtg
     4801 tagaaagttt tgctttttat gtcgtccagg taatcattta tgttttgaat atctataatg
     4861 gttcctgatt ggctcgggat aataaaaacg tttcctgagc tggttataaa gcagcggtgc
     4921 agttttcgtt ccaatggcat tattatgtca agagtctggc tgtcgaagac tccttcctct
     4981 gtaataacat agtttgagtc tgcataatat gtttcttttt ctttggctgg atttatttcg
     5041 atcaacgcta taaaattatc cagattgctg ctgtcatata cgcagcctgc cgtataaact
     5101 tttccactgt cgtagaagac catcttcttt tcctgattgt gagggaggtc tatctctttt
     5161 atgagttcca ggttatccag cttgaaagca taaagctttg attttcctgc tacataaaga
     5221 atgccttcat ccttattaac acacatgcct tctgtattat ccacccttgc agaaaagcct
     5281 ggaccttctg gtggtatggc gattatgtgc tgcccacctg ttctaacagc ggaaatatat
     5341 ataatcccgt tatataccag catgttgttc gtcatgacat taatcgtatg tttctttttt
     5401 tgctcataag tgtttttgtc atattcgtag attgtgtcga atgttcctat gtacaaggta
     5461 tctccttcca catacaggag ttttgagctt tctggtgctt caaaggtgct cgctgtctca
     5521 aaagtgccgt ttgtgaaaac ccaaatcaca ttgtctttgg ttgaaccact cattaacaga
     5581 tagacatact caccatcaac aaaatgatca atatatttct tttcaagcat aattgaaaat
     5641 tcccttttta tttttgattt tgacgtatct gttctatcca ttattgttac aaagtctggt
     5701 gacgtattaa caggtgtagg atatggaggg ataattgaat tgccatatgt tttctcaggg
     5761 gttttctcag cggttttttc gggggttttt ttttgggatt ctgatgtctt ctgtaactat
     5821 tccgcaggat gttgttacta atgcaataac aagtattaaa attaatacca gctttctcat
     5881 ttgaatcacg caccttttta ttctgtgtta attgcaatta tatcagaatt ttccaaagtg
     5941 gcaatattgt ttttaaacgc ataatctata aacgaaaggg gatgtgcgga tgattgatgt
     6001 aaaaccaatt aatctggatg gttatgtatt tattgctgtt tctgtacagc ttcctaaaac
     6061 caccctgctt gttgtttcat cagataaagg atatatcatg tgcggtgctt tggatgtttc
     6121 tttattaaat gaaaaattga aagacaggaa aattattgcg ggaagagcag ttggagttag
     6181 aactatcgag gaacttcttg aagctccttt ggagtctgtt actaatgaag ctgaaaaact
     6241 tggaataaca aaagggatgc caggtaaaaa agctctctta aaaatggttt gatttttcat
     6301 ggatataata tttttttgtg tggaaaataa tcaggggtgg ctggaatgga gaaaaatttg
     6361 acaaatggag aagtccccat tggttttgcc atggctcttg cacaaaatat gaattccatg
     6421 cagtacttct cttcattgag tcctgacaag caaagaagtg ttattggcag aaccagggga
     6481 atcaaatcaa aggcggaaat gcaggcattt gtcgacagta tttccagtat tggtatttaa
     6541 agcaaaatgg ggctgcctga cagccccatt aaagatttaa ttctttatta aagtgaatgt
     6601 acattccaag gttgcgcttt cataatcgtc tgcttcctcg acatagtttc ctttctctat
     6661 agtgatggat gcgggtaaaa tcacttgtgt aaatgtctac aaagtaaaac gaaacattgg
     6721 cgctgtattc cacggagaaa tcctcttctt aaatcaaggt gggtaattgt ccggcaacat
     6781 cctctaaaaa atatgctact gacatgctgc tgacttgaag ccaatcgcct tctgcaaggt
     6841 tactccatgt ttttatactt acaatattta tatgcggata gatggattcg ccattggatc
     6901 tcacaagtgt ttttatccct gtagcatcga acgtggtagg cgtattaatt tgcaaacacg
     6961 cccaggaaaa tgggcatttt ttcttcatgc atgatgcagt aaataaaagg tctgttcaga
     7021 tttacttcaa cttccatcca ttctggagaa cccataccac cgctgatatt ggttaccgct
     7081 gatgcggtta tgcctttttc atcaacctga atatatgtgt cttgtttaat atcggtcaag
     7141 aacaagtcga tatcggtaat ggctgagaaa tctgcgacat ctgtaaaggc atcattcagc
     7201 cctaggttct tgaccatgtc aagcaggtca agtttgcttt caaatccaaa cttgggtaaa
     7261 tgcaaagtaa ccatataccc atcaaatagt ggtgcataaa tagtggggga tttgattgct
     7321 gcttccagtt ttcccggact ggtcaggaag tcttctatac tgactccttc atcaggcagc
     7381 aagaacacca tctttccgcc tttaaccctc agggttgtac atgcatagtc ctcgccttta
     7441 tagtattctg cttgcacatc cgttcttttc atgaactcca tatctgttgt ttcagtgcat
     7501 ccataaaact tgtcgagata gttgttttct tcttcaaact cttcgttcca ttgatttcta
     7561 taatatattg tgttgattat tttaaatatg gttgtagggt catgcggaaa atctggtgca
     7621 agtgttccct cggtgttgtc aaatatccat tttttcatta gtttgcttgt atcttcatct
     7681 gtaaaatcca cactgaacaa ggttgcatag aaatgctgtc ttgcgttgtc aataaaggtt
     7741 tgtttatagg taacgccatt aagattggac agccagaccg agtttgctat ctttaacttt
     7801 ccaaactcat gatcaatgta aagtctgttg tacaatttcc ttgtctcatc catcaggaac
     7861 tcttttgaca tattgttcag tcccaacaca ttcatgagtt cctcaaaagt ttccccttcc
     7921 gcaccttgag cgcacaggct cagtgcaaag tacagactta atggactgta gtttacattc
     7981 cctccttgtt ttaacaactc gtatgcagtg ccatacgaaa atttctcaag agccaatact
     8041 gttttttgac tcacaatatt ttgaagctca aacgtaaacc ggttatcaaa ataactgtat
     8101 tctggtggat aatctgccag aaatactgtt g
//
LOCUS       DAONVY010000027         7910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6970_length_7910_cov_4.253087, whole genome shotgun sequence.
ACCESSION   DAONVY010000027 DAONVY010000000
VERSION     DAONVY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_7910_cov_4.253087"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..763)
                     /gene="atpB"
                     /locus_tag="PLI11_01395"
     CDS             complement(86..763)
                     /gene="atpB"
                     /locus_tag="PLI11_01395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPZ51549.1"
                     /translation="MPLNVDNIWILDIFGIEVWITETIVNSWIIMAILIALAIFIRIK
                     MRKFKEIPTGFQNLVEAGVEMFHNFAQNTLGEKLLYISPWFFSIFVFIFSSSLFSIFG
                     LRAPTADFATTGAFALATFILMIVLGTRHKKGEYIKSYFEPHFIFFPLNLIGELAKPV
                     SLSFRLFGNMLSGTIILTLYYSLTPIFVQIGIPAFFHAFFDVFFGAIQTYIFVIISQM
                     YVKTAAD"
     gene            complement(782..1153)
                     /locus_tag="PLI11_01400"
     CDS             complement(782..1153)
                     /locus_tag="PLI11_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPZ51550.1"
                     /translation="MKLSDLAKRMIITILVISLICVIGSVIYYRSLKFVPFLLGVLLG
                     SAVSVIKVFLLERAIDKALKMESMLAKNYVSLQHVLRLLLTGVALFLGAVVDQINLWG
                     VVCGVFAFQIATYNLKFTSKN"
     gene            complement(1174..1410)
                     /locus_tag="PLI11_01405"
     CDS             complement(1174..1410)
                     /locus_tag="PLI11_01405"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPZ51551.1"
                     /translation="MGNNNEDNNKPKISETLRALGQFTHIGITMAACVLAGVLLGKFL
                     DKLLHSSPWMVLIFSLLGAGAAIKAVFNISEKNK"
     gene            1752..5759
                     /locus_tag="PLI11_01410"
     CDS             1752..5759
                     /locus_tag="PLI11_01410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPZ51552.1"
                     /translation="MDNKAFLKTMLNLLFVGALFFLSIIFWLETGTRTNTPLSVVLLV
                     LFAFILAANIILGKRMYIKYINERITGRLKESDISIDRLERKIKISLAVIVITHIAMI
                     YFAITTMHFLNGLPNMGKTAFIPAVLFAFTVVVIGAQLWGLFDIVLNRKKKKPLQNTQ
                     ELFKKTYPNLFKIAKEAADTAKIEKDFRIFLDSSSGVKIFKSRKQYMIVVGALPMNIL
                     SMEELKQVLIHQFIRVQIKTHRLERFIDKASSIYQVETRNILVNLTRFLIKAPSVLFN
                     KVAFTCLTQVNRHAEDTADAQFKQIGSNQVFVNALAKLEAFSLFEKMPCPERDIYLYE
                     SKKPIADYASKYLDIYYKYLDENKSYYMKILDTSPSFKTRREFFGIQQYMLFTKETDT
                     EYIKEQSKILALENDILKKNMTYTYTKDRDRNYVKRFAAISGYEKNPEQDIQTLLHTA
                     LCYEGVDTEKSKEILNSILEKDSKNAGANFHLGRILIQSFDKSGIEYIKKALKTNPYL
                     AKAGLNIVEDFCTKFSLFKELDDLKTSLPETQVALGKNEEFSPHDLADDYVEEITSYI
                     TQIAEDNIEKIFLVKKADLYLFLLVPTRDADLDKLSDVHDKIMNYLLMQNTNYILLIV
                     PDRSTTRRLKKKVKNSYVWSIYGLHKKKIKDFVSQEDNWYLRQQKRRGFGFFISLYTL
                     AGLTSFGVYIDLPYHKHIAVAIGTLLLSSFAINLLLGKRLWERFSNTRIAEQRKIMGQ
                     IRDEAEKDLKRIEKRMKLILNTCWFFHIFLILLCVLGFSFSFGFVKWESWPAGLTITA
                     FLLVYLLSSFMLAGLVDMLACGGSGPLPENELLEKDYPKLFSIARGAADITGAGKNIR
                     IFLGDNNVGVFKHGKYDCILIGEMPMNLLTMEEMKQTLIHEFAHVYNGNTKLSRKLIF
                     AAGIWQTETDNKFINFLRSIMIIPAFIFQKEYFLYTVCAKRVQEIEADNIVKKLGDRQ
                     VYINALAKHAALSLFQSAPCPEWSIYIYESEEPLKNYASIYLDLYYKYLEKNEAHWRR
                     IMDNELTPFIDSHPTFKARKESMGVENYDLHTKETDKEYKKEQKKMLKKKNKILVEGI
                     ADTYDEIRDYVYVKRFDAIKDYEQNSDKSIQELITIALYYEGVDTEKAKEILNTILEK
                     DKDNAYAYYHLGRILLDEFDKQGIDYIYKAIERNDGFAVEGFDLIGTYCIKAGLQEEL
                     EEYRRNIVDMGQKAVDKSRAIAELNKEDKVVPHDLDKKTLDEILQYMTTEGKGKLAKI
                     MLVKKIAGELYTYVFLIKFANYEDEDLPEIFNKIYTYLDLREEQFTLLAIFDESKLKW
                     LHKRVENCVVWSDMQ"
     gene            5778..7835
                     /locus_tag="PLI11_01415"
     CDS             5778..7835
                     /locus_tag="PLI11_01415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPZ51553.1"
                     /translation="MNPKTKQFVTFALTIILILASMFFAVVVKPPESAIVVAILFVLL
                     MAVIAADLVLSTRLIKRFNNTKLVKRREILEETWDKAGIDIKAMEKRIKRYLNTCRLL
                     HVLFVLLCMASIYCSVIFLDTDIFVLMIMIRILFLLSSFALWGLVSLFVFKNKQELPK
                     YELSPKEYPILFQIAKEAAQLAGAGGNIRIFAGEHNICVFRHGRYDCIQIGEIIGNLL
                     TKEEMKQVLVHEFAHVYNNHTRLMNKLGNTAYNFYLHEEEDMTCLPPMRTVAFLFNRE
                     YYYYSICTRQVQEAEADNIVKKVGDRQSFVNAIAKSAAFSLFCELPCPHLSIHVFESE
                     KPIDNYGEVYLDIYLKYLEKNENLYRKILDNELPGFLRTHPIFRERKTFMEVEDFDFH
                     TIETDEEYKKEQKKLLGILSKAFDELDNYSEIRHEYYVKRFGAIKNYEENANQEVHTL
                     LETALYFEGVDNEKAKKILKTVLEKDDDNSNALFSLGRILLYEFDKRGIEYIYKAIRL
                     NEKLAQPGLQLTGRFCEMTGLEEELEEHRSKFLELGQEAMDKMEVMSGISGKDTLLPH
                     DLDKDTLDKVVQFITDTADGKLGSLLLFKKVAGELQSHVFVLVFPPNIKKKDLDDICI
                     KVHNYLDLRIEHFILLRMFQTDNVRWIYRKVKNCEVWSRKKKKVTKNIVKILTCFWVF
                     GIV"
BASE COUNT         2684 a         1519 c         1541 g         2166 t
ORIGIN      
        1 tttgctaatg cttccattat aaattcctcc attcattttc tatctatata tattatttat
       61 attttggcat atttaattaa acactttaat ccgctgcggt tttaacatac atctgactta
      121 taatcacaaa aatgtatgtt tggatggcac caaaaaatac atcaaaaaac gcatggaaaa
      181 aagcaggtat tcctatctgc acaaagatag gtgtaaggga ataatacaag gttaaaataa
      241 tggttcctga gagcatgtta ccaaaaagcc tgaaactcaa agataccggc tttgccagct
      301 ctccaattag attcaatggg aagaatatga aatgaggttc aaaataactt ttaatgtatt
      361 ctcccttttt atgtctggtt cccagtacta tcatgagaat aaatgtcgca agagcaaatg
      421 ctcctgtggt tgcaaagtca gctgttggag ctctaagtcc aaatatgctg aacagactgg
      481 aagagaaaat aaatacaaat attgaaaaga accacgggga tatgtataac agtttttccc
      541 ccagagtatt ctgggcaaaa ttatgaaaca tttcaacccc tgcttccacc aggttctgaa
      601 aaccggtggg tatctccttg aatttacgca ttttgattcg gataaaaatt gcaagtgcaa
      661 taaggatagc cattataatc caggagttga ctatcgtctc tgttatccaa acctctatac
      721 caaatatgtc caatatccat atgttgtcaa cattcaatgg cacttgccta caccaccttg
      781 tctagttctt tgatgtgaac tttaaattgt atgtcgcaat ctgaaaggcg aacactccgc
      841 aaacaacacc ccacagatta atctgatcaa ccactgcgcc taaaaacaga gcgacacctg
      901 ttaaaagcag tcttaatacg tgctgaagac ttacgtagtt tttcgcaagc atggattcca
      961 ttttcaaagc cttgtctatt gcgcgctcca aaagaaatac ctttattacg cttacagcgc
     1021 tacccagtaa aacaccaagc aaaaagggga caaacttaag tgaccgatag taaattaccg
     1081 aacctatcac acagattaga gatataacca gtattgtaat tatcattctt tttgccagat
     1141 cagacaactt catataatct ttctcattcc ttgctactta ttcttctccg aaatattgaa
     1201 tacggctttg attgctgctc cagcacccaa caacgaaaaa atcaaaacca tccacggcga
     1261 cgagtggagc agtttgtcta gaaacttgcc aagcagaaca cccgcaagta cacatgccgc
     1321 catcgtaatc ccaatatgtg taaactgccc caaagcccgc agtgtttcac ttattttggg
     1381 cttgttgttg tcctcattgt tattacccaa tttgttcccc tcactttact tatggtaccc
     1441 ctgtaatata cttttagaca gtataaatat taccaaaata ttgatgaggt atcaagttat
     1501 cgtgcctgta agtgtatgta cataaggagg ggcatttgca gccatatcag attctgggca
     1561 taagaatatt ataccataat ttatgattaa aataaaagaa aaatgtaaac tgtgaataaa
     1621 ttttcttcga attatacaat tttataatta cattattctg ctgacgtact tcggtatgtt
     1681 ttaccatgtt gtataaacac cctttgtatg ctagaatatt ttccatactt atacaatttg
     1741 tggaggaata catggataat aaggcgtttt taaaaacaat gctaaatctc ttgttcgtag
     1801 gagctctttt tttcttaagt atcatattct ggttagaaac aggaaccaga acaaacacac
     1861 cactgtctgt agttttgctc gtgttgtttg cattcatact tgcagcaaac attattcttg
     1921 gaaaacgcat gtatataaaa tacataaacg aaagaataac agggcgttta aaagaaagcg
     1981 atatcagtat tgacaggctt gaaagaaaaa taaaaatatc ccttgcagtt attgtaataa
     2041 ctcatattgc aatgatatat tttgccatta ccaccatgca ttttctgaac ggactgccaa
     2101 acatgggtaa aacagccttc attccagctg tactttttgc ttttaccgtg gttgttatcg
     2161 gcgctcaatt atgggggctt ttcgacattg ttctaaatag aaagaaaaag aagcctttac
     2221 agaacacaca agaactattt aaaaaaacct atccaaatct gtttaaaata gcaaaggaag
     2281 ctgcagacac tgcaaaaatt gaaaaggact ttcgcatttt tcttgatagc agcagcggtg
     2341 taaagatttt taaatcccgc aaacaataca tgatcgtcgt tggcgctctt ccaatgaaca
     2401 ttctatccat ggaagagctg aaacaagttc ttatacacca attcatccgt gtacaaataa
     2461 aaacccaccg ccttgaaaga tttatcgaca aggcttcttc catctatcag gtggaaacac
     2521 gaaacattct ggtaaacctg accagatttc taattaaagc accctctgtt ctttttaaca
     2581 aagtagcatt cacatgcttg acacaagtaa acagacatgc cgaagataca gcggacgctc
     2641 aattcaagca aattggcagc aatcaagtat tcgtcaacgc ccttgcaaag cttgaagctt
     2701 tctctctgtt tgaaaaaatg ccctgccctg aaagagacat atacctgtac gaaagtaaaa
     2761 agcccattgc cgactatgca agcaaatacc tcgacatata ttataaatac cttgatgaaa
     2821 ataaaagtta ctacatgaaa attctggaca cctctccatc tttcaaaacg agaagagagt
     2881 ttttcggaat tcaacaatac atgcttttca caaaagaaac tgatactgaa tacataaagg
     2941 agcaaagcaa gattcttgcc cttgaaaacg atattttaaa gaaaaacatg acatacacat
     3001 acacgaaaga cagagaccgc aattatgtga aaaggtttgc agccatatcc gggtatgaga
     3061 aaaatcccga gcaggatata caaacacttt tacacaccgc cctgtgctat gagggtgttg
     3121 ataccgaaaa gtcaaaggag atattgaatt ccatacttga aaaagacagt aaaaatgcag
     3181 gcgcaaactt ccatcttgga agaattttaa ttcaaagttt tgacaaaagc ggaattgaat
     3241 atatcaaaaa agctttgaag acaaacccct accttgcaaa ggcagggctt aatatcgttg
     3301 aagatttctg cacaaagttc tcccttttca aagaacttga cgatttaaaa accagccttc
     3361 cagaaacaca ggtcgcactc ggcaaaaacg aagagttttc accccacgac ctggctgatg
     3421 attacgttga agaaatcaca agctacatca cgcagattgc tgaagacaat attgaaaaga
     3481 tattccttgt aaaaaaagcg gatttgtatt tgttcctatt agtgccaaca agggatgctg
     3541 atcttgacaa gctatcagat gttcatgata aaatcatgaa ttatcttctt atgcaaaaca
     3601 caaattacat acttttaatc gttcctgaca gaagcacaac aagacgatta aagaagaaag
     3661 taaagaacag ctatgtatgg tcaatttacg gtctacacaa gaagaaaatc aaggattttg
     3721 ttagtcagga agacaactgg tatttgaggc agcagaaaag gagaggtttt ggttttttta
     3781 tttccctcta cactttggca ggtttaacat cgtttggggt atatatagac ctgccatatc
     3841 ataagcacat cgcagtcgca attggtacac tgcttctgtc ttccttcgct ataaatctgc
     3901 ttctgggaaa acgcctctgg gaaagatttt caaacacaag aattgcggaa cagaggaaga
     3961 taatgggaca gatcagggat gaggcagaaa aagatctgaa aagaatagaa aaaagaatga
     4021 aactcatcct gaatacctgc tggtttttcc acatattttt aatactgctg tgcgtcctgg
     4081 gattttcctt ctcctttgga tttgtaaagt gggagagttg gcctgctggt ttaactataa
     4141 ctgcattttt gttagtatat ttactgtcct ctttcatgtt ggcaggcctt gtcgacatgc
     4201 tggcatgcgg tggttcaggt ccacttcctg aaaatgaact tttggaaaaa gactatccaa
     4261 aactcttttc tattgcaaga ggagcagcag acattaccgg tgctggcaaa aacatacgta
     4321 tttttcttgg cgacaacaac gtgggagttt tcaagcacgg gaagtatgac tgcatattga
     4381 ttggagaaat gcccatgaac ctcctaacca tggaagaaat gaagcagact ctcatccatg
     4441 agtttgccca tgtttacaat ggaaacacaa agctttcaag aaaacttata tttgcagcag
     4501 gaatttggca gacggaaacg gataacaaat ttataaactt tttaagatcc attatgataa
     4561 tacctgcgtt catcttccaa aaggaatact tcctctatac cgtttgtgca aagagagtcc
     4621 aggaaattga agctgataat attgttaaga aactgggcga cagacaggta tacataaacg
     4681 cccttgcaaa gcacgccgct ttgagcttgt ttcaaagtgc accctgcccg gaatggtcaa
     4741 tatacatata tgagagcgag gagcctctga agaactatgc aagtatctat ctcgacctct
     4801 actacaaata ccttgagaaa aacgaagccc attggcggcg cataatggac aacgaactta
     4861 ctcctttcat tgattcccac cccactttca aggccagaaa agaatcaatg ggtgttgaga
     4921 actacgactt gcacacaaag gaaacagaca aggaatacaa aaaagaacag aaaaagatgc
     4981 tgaaaaaaaa gaacaaaatc ctggtagaag gcattgcaga tacatacgat gagattcggg
     5041 attatgtata cgtaaagaga tttgatgcaa tcaaggatta tgaacagaat tctgacaaga
     5101 gcatacagga gctcataacc attgccctgt actacgaagg agtggacacg gaaaaggcaa
     5161 aggaaatact aaacacaatc cttgaaaaag acaaggacaa tgcctatgcg tattaccacc
     5221 tgggcaggat cctgcttgat gagtttgaca agcagggtat tgactacatc tacaaagcca
     5281 ttgaaaggaa cgatggtttt gcagttgaag gctttgacct gatcggcacc tactgcataa
     5341 aggcaggact ccaggaagag ctggaggaat acagaagaaa catagttgac atgggtcaga
     5401 aggccgtaga caaaagcagg gcaattgcag agttgaataa ggaagacaag gttgtacccc
     5461 atgacttgga caaaaaaacc cttgatgaga ttcttcagta catgacaacc gagggcaagg
     5521 gaaaactggc aaaaatcatg ctggtaaaga aaattgcggg tgagctgtac acctatgttt
     5581 tccttatcaa atttgcaaac tatgaagatg aggatctgcc ggagatattt aataaaatat
     5641 atacgtatct tgatttgagg gaagaacagt ttaccctgct ggccattttc gatgaatcaa
     5701 aactgaagtg gctgcacaaa agggttgaaa actgtgttgt ttggtcggat atgcaataga
     5761 ataacaaggt gattgtattg aacccaaaaa ctaaacagtt tgtcacattt gcattaacta
     5821 taattcttat acttgcttcc atgttttttg cagttgtggt taaacctcct gaatctgcaa
     5881 ttgttgtggc catactcttt gttctgctta tggcagtcat tgcagcggat ttagttttga
     5941 gcaccaggct gataaaacgg tttaacaaca caaaactggt aaaaagaaga gaaatccttg
     6001 aagagacatg ggataaagcc ggaatagata ttaaagccat ggaaaaaagg ataaaaaggt
     6061 atctcaatac atgcaggctt ttacatgttc tttttgtcct tttatgcatg gcctccatat
     6121 actgctcagt tatattcctg gatacagaca tctttgttct catgattatg atacgcatcc
     6181 tttttttgct cagcagtttt gcattatggg gactggtttc cctgttcgta ttcaaaaaca
     6241 agcaggaatt acctaaatat gagttgtctc caaaagaata tccgatactt ttccagatag
     6301 caaaagaggc tgcacaattg gccggcgcgg gtggaaatat aaggatattt gcaggagagc
     6361 acaacatttg tgtgttcagg catggaagat atgactgcat acagatcgga gaaattattg
     6421 gaaacctgct taccaaagaa gaaatgaagc aagtccttgt tcatgagttt gcccatgtgt
     6481 ataacaacca cacaagactt atgaacaagc ttggcaatac cgcatacaac ttctacttgc
     6541 atgaagaaga agacatgaca tgcctgcccc caatgaggac agttgcgttt ttattcaacc
     6601 gggaatatta ttattactca atctgcacaa gacaggtgca ggaagcagaa gcggacaata
     6661 ttgtaaaaaa ggtgggtgac aggcagtctt tcgtaaacgc catagcaaaa tccgcagcat
     6721 tctcattatt ctgtgaactt ccttgtccac acctttcaat tcatgtcttc gaaagcgaga
     6781 aaccgattga caattatgga gaggtgtatc ttgatatata tctaaagtat cttgagaaga
     6841 acgaaaacct ttacagaaag atactggata atgaattgcc tggcttttta aggacccacc
     6901 caatctttcg ggaaagaaag actttcatgg aagttgagga ctttgacttt catactatag
     6961 agaccgacga ggaatacaaa aaggaacaga aaaagctgct tggaatattg agtaaagcct
     7021 ttgatgaact tgacaactac agcgagataa ggcatgagta ttatgtcaaa aggtttggag
     7081 ccataaaaaa ctacgaagaa aatgcaaacc aggaagttca tactcttttg gagaccgccc
     7141 tctatttcga aggtgttgac aatgaaaaag ccaagaaaat tctgaaaacc gtacttgaaa
     7201 aggacgatga caactccaac gccctctttt ccttgggaag aattcttctt tatgagtttg
     7261 ataaaagagg tattgagtac atatacaagg caattagatt aaatgagaaa cttgctcaac
     7321 ccgggctcca gctgacaggc cgtttctgcg aaatgacggg attggaagaa gaactcgagg
     7381 aacacagaag caagttttta gagcttggac aggaggccat ggacaaaatg gaagtgatgt
     7441 ccggtatatc gggtaaagac actctccttc cccacgacct tgacaaggat accttggata
     7501 aagttgtgca attcataacc gacaccgcag atggcaagct gggcagcctt cttcttttca
     7561 aaaaagtggc gggagagttg caaagccatg tttttgttct tgtattccca ccaaatatca
     7621 agaagaagga cttggatgat atttgtataa aagtgcataa ttaccttgat ctaagaattg
     7681 agcattttat tctccttcga atgtttcaga ccgataatgt gagatggata taccgtaaag
     7741 taaaaaactg cgaggtttgg tcaagaaaaa agaaaaaagt tacgaaaaat atcgttaaaa
     7801 ttttaacttg cttttgggtt ttcggtatag tataatactg agcgcaggca atctgcgagg
     7861 attttccttt cagaaagcct gaaaagaaaa aagacaattg tactgacatg
//
LOCUS       DAONVY010000028         7907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_6975_length_7907_cov_3.570937, whole genome shotgun sequence.
ACCESSION   DAONVY010000028 DAONVY010000000
VERSION     DAONVY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6975_length_7907_cov_3.570937"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLI11_01420"
     CDS             <1..292
                     /locus_tag="PLI11_01420"
                     /inference="COORDINATES: protein motif:HMM:NF018401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HPZ51554.1"
                     /translation="TIMGCIMIFGILTDRLASVINSLSGIYGSANLSGEEAVTLMKIM
                     GIAYVTEFASSTCKDAEQTAIAGKIELAGRIFIVWLALPLAVKLLSTLKTLF"
     gene            235..1323
                     /locus_tag="PLI11_01425"
     CDS             235..1323
                     /locus_tag="PLI11_01425"
                     /inference="COORDINATES: protein motif:HMM:NF021088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51555.1"
                     /translation="MAGFATGCKTFVHAEDSVLRAGDIMDNTVINELKDKLDTAGSLW
                     EGLLDPVAIMENAMEGRMNLSFGEILLKVMGELFKNLAGNVDTVLYIIGITYLTVFIF
                     SVSAPDKASWSETVLIVAAAILAVPAVADFVKVVGLANEAMTKMSAITVASIPILSAV
                     SLNSQGAVFLIIAQSVTLVIKSILLPLAVIFGALGFCEVISDKFSVTGIRTIIRSTFN
                     WILGITMVVFACSASVSGIVANSFTSISTRTLKYAGSMVPVVGRYLAESADLVFSGAG
                     ILKDAGGVGVMAAVFVACLSPFIKILSIVILYRLAVVFVSPVAHEKISKVITLVADAL
                     SMIMSITALLGVMYIINIAVMVSLGRSL"
     gene            1320..1955
                     /locus_tag="PLI11_01430"
     CDS             1320..1955
                     /locus_tag="PLI11_01430"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HPZ51556.1"
                     /translation="MKEWLFTIIVSSFLLTMLLQLVPDGNIKKTCKVAFGFVFLAVII
                     SPLTDLADGFFKNINNNKIQVNLAYSELLAGVKDAEEYNMYTDEVMNQYVNRLEKEVN
                     DALMGIGVVSDTVIVVNRDIESDSFGFVASVSCNVRLEDGKKEKKSIVKPIDKIDKIV
                     IGTDGIKVKKHKDEEGEKKISEEKKEKEKEIRYVVSLLLGVDEKNISIDWV"
     gene            1971..2570
                     /locus_tag="PLI11_01435"
     CDS             1971..2570
                     /locus_tag="PLI11_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51557.1"
                     /translation="MDLKNNIEVIKGFVTKYKKIIIMVVVGCLLIILGNMLLDVSGGK
                     ESPKMFLPQATKTPGISEIESGMELMGPDEYANYLQKQMEDLLSTIEGVGKVKVLVYV
                     DESCELVPAYDSEKNESNLEEQDSEGGKRKQNDYRERKDVFDNNGTVVLKVVYPKVKG
                     IVVSAQGARSNVVKERIMNALVALYGIEPHRVEILSMKS"
     gene            2626..3156
                     /locus_tag="PLI11_01440"
     CDS             2626..3156
                     /locus_tag="PLI11_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HPZ51558.1"
                     /translation="MMSLKTKRLIALGICLVLLAAAIFQNIRNNKDKAAGKDDDKKGL
                     IVNDPDDYLDETILDAEEFFAGARLERESKRSLLVAECAAVIDDTESSEEEKVQAMEL
                     KDAIEMMIELESDMETAIKGRGYEEVIVELDEDGYIYVTVMAEELTEQEVMVLAGIAI
                     DTTGATLDQIVVKNIS"
     gene            3313..3672
                     /locus_tag="PLI11_01445"
     CDS             3313..3672
                     /locus_tag="PLI11_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPZ51559.1"
                     /translation="MMNGYPEENDKEDILITDEVIAVIAGIACSEVEGVASMSPGIAG
                     GLVEAFGGENPAKGVKVETKNDETTIDLYVIVKYGYRIPDLAWKIQEKVKDTVEALTS
                     ISVPVVNIHVQGVDFGE"
     gene            3665..4210
                     /gene="amaP"
                     /locus_tag="PLI11_01450"
     CDS             3665..4210
                     /gene="amaP"
                     /locus_tag="PLI11_01450"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HPZ51560.1"
                     /translation="MSNYLFRVLAVIYAFLASVASAVIMISPFGDKILMEYIIEYTNA
                     NFFQSNRYDIMLFIIGLVFLCLNVFFLTSGIKNTRSSKYLVTENESGVVRISTTAIEN
                     VANSLSRRFQGVKDAKAKAYFKKDKVNIIVKLSVMPDVHVPNLCKSIQDRIKGSVEVT
                     MDLEVQNISVSVDSVYTATQS"
     gene            4220..4447
                     /locus_tag="PLI11_01455"
     CDS             4220..4447
                     /locus_tag="PLI11_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HPZ51561.1"
                     /translation="MFKRIYKFYRRNFHSINGTIIGLLIGLSFVFLGIFRTLFLIFCM
                     LVGNYVGKKIQKDKDFIKNLLDKILPPGSYR"
     gene            4469..4936
                     /gene="nusB"
                     /locus_tag="PLI11_01460"
     CDS             4469..4936
                     /gene="nusB"
                     /locus_tag="PLI11_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPZ51562.1"
                     /translation="MGRRESRDAAVKMIFQQGFQPDEEIMTEAMISTFIDNAQYFDDD
                     DTEKENDLNYTKELDMPYLKNVLDGVFDNLDRLDTMINKHAKGWTISRMARIDVAILR
                     VAVYEILYREDIPFSVSVNEAVELAKKYSHDGAGSFINGILASIISEIEGANG"
     gene            4929..6128
                     /gene="xseA"
                     /locus_tag="PLI11_01465"
     CDS             4929..6128
                     /gene="xseA"
                     /locus_tag="PLI11_01465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPZ51563.1"
                     /translation="MDKYFTVSQINSYIKTLLQNDRNLTNIWIVGEISNYKLHTTGHS
                     YFTLKDPEGLIRCVMFRSRNLRLKFQPENGMKVLAYGSVSVYERDGSYQFYVDTMEPF
                     GLGALHIAYEQLKEKLQKEGLFDSERKKKIPFLPSCIGVVTSKSGSVIKDIMNVTYRR
                     FPTMKIVLFPVQVQGEMASYQVENALKLAERLGICDVLIVARGGGSMEELWAFNEERT
                     VRAISECSIPVISAVGHETDFTLSDFAADLRAPTPSAAAELAVPELRHLKYTLETYFN
                     KIQDIPLGNILLKRKELDRVKHSAVFRRPLDIIQRENMNLGVMADKLNSLFSYGFEMK
                     KQRFNSLNDVLYQLDPYKILERGYSVARKKDGKVIDSVSDISEGEVFEIHVKDGSFDA
                     RRENNNE"
     gene            6121..6348
                     /gene="xseB"
                     /locus_tag="PLI11_01470"
     CDS             6121..6348
                     /gene="xseB"
                     /locus_tag="PLI11_01470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPZ51564.1"
                     /translation="MNEEMSFEEALAKIEEIIKTMESGKLPLEETIAKYQEGVKLINY
                     CQAKLDSYEKIVTAITENNGVVTEEEVFSDI"
     gene            6338..7228
                     /locus_tag="PLI11_01475"
     CDS             6338..7228
                     /locus_tag="PLI11_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPZ51565.1"
                     /translation="MTFEKKFDEFVDEINENLDKLVVEKDNLQKDIYTAMRYSLLAGG
                     KRIRPVILLACAEILDIDKDTVMPFACAIEMIHTYSLIHDDLPALDNDELRRGRNTCH
                     IEFSEALAILAGDALLNKAFEVMSKKAIDMEHPTRGLRMLHQVSVMSGTEGMIGGQII
                     DIQSEGKEVSKEVLETMYKGKTGCLLKAPVLVALEAANCMEDENAEILINYVETIGVA
                     FQIKDDILDSIGDEKTLGKNTGRDVEKNKTTFVTIYGLEYCEELLEELTHKAIGYAEK
                     LGEKGKFLKELAVYLLKREN"
     gene            7244..>7907
                     /locus_tag="PLI11_01480"
     CDS             7244..>7907
                     /locus_tag="PLI11_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HPZ51566.1"
                     /translation="MGSLLDKLNLPEDLPKLDIETLDELAEEIRQRLVDVVSKNGGHL
                     ASNLGIVELTLGMYTVWNPYKDRIVWDVGHQCYVHKILTGRNKELATLRQLDGISGFP
                     KREENPADAFNTGHSSTSISAALGIMRGLGLQKGKSKCIAVIGDGALTGGMAFEALND
                     AARVFNDFVIILNDNGMSISRNVGGMSRYLGKIRTGRSYIRIKKGLKNTLYKIPFIGE
                     KLV"
BASE COUNT         2427 a         1269 c         2066 g         2145 t
ORIGIN      
        1 gactataatg ggctgcatta tgatatttgg aattcttaca gacaggcttg cgtccgttat
       61 aaattcactt tcaggcatct atggcagtgc aaacctttca ggagaagaag cagttactct
      121 aatgaagatt atgggaatag catacgtaac agagtttgcc tcgtccacct gcaaggacgc
      181 agagcaaaca gcaatagcag gcaagataga gcttgcgggc aggattttca ttgtatggct
      241 ggctttgcca ctggctgtaa aacttttgtc cacgctgaag actctgtttt aagggcaggt
      301 gacattatgg ataacacggt tattaacgaa cttaaggaca agttggatac ggcgggaagt
      361 ttatgggaag ggcttcttga ccctgttgcg attatggaga atgccatgga gggaaggatg
      421 aatctgtcct ttggcgagat actgttgaag gttatgggtg agttgtttaa gaatcttgca
      481 gggaatgtgg atacggtatt atacattatc gggattacat atcttactgt atttattttc
      541 tccgtatctg ctccggacaa ggcctcctgg tctgaaacgg tgctgattgt ggcagctgca
      601 atactggcag tgcctgcagt agctgatttt gtcaaagttg taggtcttgc caacgaagcc
      661 atgaccaaga tgtcagctat tacggtggcg tccattccaa ttctgtctgc tgtttcacta
      721 aacagccagg gagcggtgtt tctgattatt gcccagtcag tgactttggt aattaaaagc
      781 atactgctgc ctctggccgt tatattcgga gctctgggat tctgcgaggt catatcggac
      841 aagttcagtg taaccggaat aagaacaata ataagaagca ccttcaactg gatattgggt
      901 attacgatgg tggtgtttgc ctgcagcgcc tctgtgtccg gcatagtggc aaatagtttt
      961 accagcataa gtacgagaac attaaaatat gcgggaagca tggtgcctgt ggtgggaaga
     1021 tacctggccg agtctgcgga cctggtgttt tcaggagcag gtatacttaa ggatgcaggg
     1081 ggcgtggggg tcatggcagc ggtatttgtg gcgtgtcttt ctccgtttat taagattttg
     1141 tcaattgtaa tattgtacag gctggctgtc gtttttgtat caccggttgc ccatgaaaag
     1201 attagcaagg tgattacact ggttgctgat gcattgtcca tgataatgtc aatcacagca
     1261 ctgcttggtg taatgtatat tataaatatt gccgtcatgg tttctcttgg gaggtcgtta
     1321 tgaaagagtg gttgtttaca ataattgtat cctcatttct tcttaccatg cttttgcagc
     1381 tggtgccgga tggaaatatc aagaaaacat gcaaggtagc ctttgggttt gtttttctcg
     1441 cagtgattat ttctcctctg acagatctgg cggacggttt tttcaagaac atcaataaca
     1501 acaagataca agtcaatctg gcatattcgg aactgcttgc aggtgtgaag gatgctgagg
     1561 aatacaatat gtatacggat gaggttatga atcaatacgt caacagactt gaaaaagagg
     1621 ttaacgatgc cttgatggga attggagttg tgagcgatac agtgattgtg gttaacaggg
     1681 atattgaatc ggacagtttt ggatttgttg catcagtttc atgtaatgtg aggctggagg
     1741 atggcaaaaa ggagaaaaag agtattgtaa agccaattga taagattgac aagattgtta
     1801 taggtacgga tggaatcaaa gttaagaaac acaaggatga ggaaggtgaa aagaagataa
     1861 gtgaggagaa gaaggaaaag gagaaggaga taagatatgt tgtgtctctt cttcttggag
     1921 ttgatgagaa gaatatatca atagactggg tttgaaacgg ggtgtggaaa atggatctta
     1981 aaaacaatat tgaagtgata aaagggtttg tcaccaaata caagaagata attataatgg
     2041 tggtggttgg gtgtttgttg attattctgg ggaacatgtt gcttgacgtt tcggggggca
     2101 aagagtcgcc taaaatgttt ttgccccagg cgaccaaaac accaggcata tcagaaattg
     2161 aaagcggcat ggagcttatg gggcctgatg aatacgcaaa ctatcttcaa aagcagatgg
     2221 aggatttgct ttcaaccatt gaaggtgttg ggaaagtgaa agttctggta tacgtggatg
     2281 agtcctgtga gctggttcct gcttatgaca gcgaaaagaa cgaatcaaac cttgaggagc
     2341 aggattccga gggaggcaag aggaagcaga atgattacag ggagagaaag gatgtctttg
     2401 acaacaacgg cactgtggtt cttaaggttg tgtatccgaa ggtgaaagga atagtggtat
     2461 ctgcccaagg cgccaggagt aatgtggtta aggagcgcat tatgaatgcc ttggtagcct
     2521 tatatggaat tgagcctcac agggttgaaa ttcttagcat gaagagctga cacgcatcat
     2581 atatttatgt ataattagaa gtttattgag cttgatggag gggttatgat gagtcttaaa
     2641 acaaaacggt tgatagcgct tggcatatgt ctggtacttc tggcagctgc catatttcag
     2701 aatatcagga acaacaagga caaggcggca ggcaaggatg acgacaagaa gggtctcata
     2761 gtgaatgatc ctgacgatta tctcgatgag accattctgg atgcggagga gttctttgct
     2821 ggagcaagat tggaaagaga gagcaaaaga agtcttttgg tagcggagtg tgctgcagtt
     2881 attgatgata cggaatcatc ggaggaagaa aaggtgcagg ccatggagct taaggatgcc
     2941 attgagatga tgatcgaact tgaatccgac atggagactg ccattaaagg aagaggttat
     3001 gaggaagtca ttgtagagct tgatgaagat ggatatattt atgtgacagt catggctgaa
     3061 gagcttaccg agcaggaagt gatggtactt gcaggcatag caattgacac cacaggagca
     3121 actcttgatc agatcgtagt caagaacata agttgaagaa tgtaagacat atatgtagag
     3181 gtggcgcagt gtagtttgca ttgcgccatt tttacttttt attatgactg ttttgtcata
     3241 atgtgttata attatctgcg taaacagata ctagggggat taaacttgga caagtccttt
     3301 gatgatgaca atatgatgaa tggttatccc gaagaaaatg acaaggaaga catactcata
     3361 acggacgagg ttatagccgt tatagcgggg attgcctgtt ccgaagtgga gggggttgct
     3421 tccatgagtc ctggaatagc cggtggtctg gtagaagcct ttggagggga gaatcctgcc
     3481 aaaggtgtga aggttgaaac aaagaatgat gaaacaacga tagatttgta tgtaattgtg
     3541 aagtatggat acaggatacc tgatcttgcc tggaagatac aagagaaggt aaaggatact
     3601 gtggaggcgc ttacaagcat ctcggttccg gtagtcaata tacatgtaca aggagtggat
     3661 tttggtgagt aattatctgt ttagggtttt ggcggtaatt tatgcatttt tagcgtcggt
     3721 tgcaagcgct gttattatga tttcgccttt tggcgacaag atattgatgg aatacattat
     3781 cgagtacact aacgcaaact tttttcaatc aaacaggtat gacattatgt tgtttattat
     3841 cggtcttgtt ttcttgtgtc tgaatgtgtt cttcctgacc tccggaatca aaaacaccag
     3901 atcgtcaaaa tatctggtaa ccgagaatga gtcgggagtt gtcaggattt ccactactgc
     3961 cattgaaaat gttgcaaact ccctgtccag aagattccag ggtgtaaagg atgccaaagc
     4021 gaaagcatat ttcaagaagg ataaagtaaa tataatagtg aaactgtcgg taatgccaga
     4081 cgtgcacgtg ccgaacctct gcaaaagcat tcaagataga atcaagggct ctgttgaagt
     4141 taccatggat cttgaggtgc agaacataag tgttagtgtg gacagcgttt atacggccac
     4201 tcagagttag gagggtgtca tgttcaaacg gatttataaa ttctaccgga gaaatttcca
     4261 cagcataaac ggcaccatta taggtctgtt gataggactt agttttgtgt ttttagggat
     4321 attcagaacc ctgtttttaa tattctgcat gcttgttgga aattatgttg gaaagaagat
     4381 acagaaagac aaggatttca taaagaattt gttggataaa atactaccac ccggatcata
     4441 cagataatat aatggaggaa gttgtttaat gggaagacga gagtcaagag atgcagcagt
     4501 aaaaatgata tttcaacaag gttttcagcc tgatgaagag ataatgacgg aggcaatgat
     4561 ctccacattt attgataatg cccaatactt tgacgatgac gatactgaga aagagaatga
     4621 tttgaattat acaaaagaac tggacatgcc ctatttgaaa aatgttttgg atggtgtgtt
     4681 tgataatctg gatagattgg acaccatgat aaacaagcac gcaaaaggct ggaccatttc
     4741 aagaatggcc cgcattgatg tggcaatttt aagggtggct gtctacgaga tactatacag
     4801 agaggacata cccttcagtg tttcggtaaa cgaggctgta gagcttgcga aaaagtacag
     4861 tcatgacggt gcaggctcgt ttataaacgg catacttgcc agcattatca gcgagataga
     4921 gggtgcaaat ggataaatat tttacagtca gccaaataaa ctcatatatc aaaacacttc
     4981 tgcagaatga cagaaatctc accaatatct ggattgttgg agaaatatca aactacaagc
     5041 tccatacaac cggccattca tattttaccc ttaaagaccc tgaaggcctc atccgctgcg
     5101 tcatgtttag aagtagaaac cttcgcctga agttccagcc cgagaatggg atgaaagtgc
     5161 tggcttacgg cagcgtttca gtatatgaaa gggatgggag ctatcagttt tatgtagata
     5221 caatggaacc ctttggtttg ggagccttac atatcgcata cgagcaattg aaggagaaac
     5281 ttcaaaagga agggttgttt gattccgaaa gaaagaagaa aatacccttt cttccgtcat
     5341 gtataggtgt agttacatca aaaagcggct cagttattaa agacataatg aatgtaacct
     5401 acagaagatt tccaacaatg aaaattgtac tgtttccagt acaggtccag ggagagatgg
     5461 cctcctatca agttgagaat gccttgaaac ttgcagaaag gcttggaata tgcgatgttt
     5521 tgattgtggc aagaggtgga ggttccatgg aggaactgtg ggcttttaat gaagaaagga
     5581 cagtacgagc aataagtgaa tgttcaattc ctgtaatatc agccgttgga catgaaacgg
     5641 atttcacact ttcggatttt gcagcggact tgagagcccc tacgccttct gctgccgcag
     5701 aacttgcagt gccggagctt aggcatctta agtatacttt ggagacctac tttaacaaga
     5761 tacaggatat tccgctgggg aatatcctgc tgaaaagaaa ggagttggac agagtaaagc
     5821 actctgcggt cttcagaagg ccattggata taattcagag ggagaatatg aatcttggtg
     5881 ttatggcgga caagctaaac agtttgtttt cttatggttt tgaaatgaaa aagcaaagat
     5941 tcaacagttt gaatgatgta ctgtatcaac tggatccgta caagattttg gaaagaggat
     6001 acagtgtagc caggaaaaaa gacggcaagg taattgattc tgtttcagat atttccgagg
     6061 gtgaagtttt tgagattcat gtaaaggacg gaagctttga tgcaaggagg gagaataata
     6121 atgaatgagg aaatgagttt tgaagaggct cttgccaaaa ttgaggagat tataaaaacg
     6181 atggaaagtg gcaaattacc tcttgaggag actattgcaa aatatcagga gggcgtgaaa
     6241 ctaattaatt actgtcaagc caaactggat tcttatgaga aaatagttac cgcaataaca
     6301 gaaaacaatg gagtagttac cgaggaggaa gtttttagtg acatttgaaa aaaagtttga
     6361 tgaatttgtt gatgagataa atgaaaatct ggacaagctg gttgttgaaa aagacaacct
     6421 gcaaaaagat atatacacgg cgatgagata cagcctgctt gctggaggga aaaggataag
     6481 gcctgttatt ctgcttgcat gtgcagaaat acttgatata gacaaggaca ctgtgatgcc
     6541 gtttgcatgt gccattgaga tgatacatac gtattctctc atacatgacg acctgccagc
     6601 ccttgacaat gacgaactta gaaggggaag gaacacttgc catatagagt tttcggaggc
     6661 tcttgcaatt cttgcaggag atgctctttt aaacaaggct tttgaggtaa tgagtaaaaa
     6721 ggcaatagat atggagcatc caacaagggg gttgagaatg ctgcatcagg taagtgtcat
     6781 gtctggaacg gaaggaatga ttggaggaca gattattgac atccagtccg aaggcaaaga
     6841 ggtttcaaaa gaggtcctgg aaaccatgta caagggcaag acagggtgtc ttttgaaggc
     6901 gcctgttttg gttgcacttg aggctgctaa ctgcatggag gatgaaaatg ccgaaatcct
     6961 gataaactat gtggagacaa taggagtggc atttcaaata aaagatgata tacttgacag
     7021 tataggagat gaaaagactc ttgggaagaa taccggcagg gacgtggaga agaacaagac
     7081 cacgtttgtg acgatttacg gacttgagta ttgcgaggaa cttctcgaag aactgacaca
     7141 caaggcaatt ggttatgctg aaaaactggg agaaaagggc aagtttttaa aggagcttgc
     7201 tgtttatctt ttgaagcgag aaaattaaaa ggaagggaat ttaatgggga gtcttcttga
     7261 caaacttaat ctacctgaag atttgccaaa gcttgacata gaaacccttg atgagctggc
     7321 cgaggagata agacagaggc tggtggatgt ggtaagcaag aatggcggac atcttgcatc
     7381 caatttgggt attgttgagc tgaccctcgg catgtatacg gtctggaacc cttacaagga
     7441 cagaatcgta tgggatgtgg gtcaccaatg ttatgttcac aagatactta caggtagaaa
     7501 caaggagctt gccaccttgc ggcagcttga cggcattagc ggttttccca aaagagagga
     7561 aaatcctgca gatgctttca atactggaca cagttccaca tccatatcgg cagcgcttgg
     7621 cataatgcgt ggacttggtc tgcaaaaagg aaagtcaaaa tgcatagccg tcataggtga
     7681 cggagcactt actggcggaa tggcatttga ggctttgaat gacgctgcca gggtttttaa
     7741 tgattttgtc attattctca atgacaatgg aatgagcata tcaagaaacg taggcggcat
     7801 gtcgcgttat ttgggaaaaa tcagaaccgg acgctcatat attcgcatca aaaagggttt
     7861 gaagaacacc ttgtacaaga ttccttttat aggcgaaaag cttgtaa
//
LOCUS       DAONVY010000029         7792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7096_length_7792_cov_4.006721, whole genome shotgun sequence.
ACCESSION   DAONVY010000029 DAONVY010000000
VERSION     DAONVY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7096_length_7792_cov_4.006721"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..1218
                     /locus_tag="PLI11_01485"
     CDS             511..1218
                     /locus_tag="PLI11_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ51567.1"
                     /translation="MKKIVFLSFILVIALASQLSVMAANNIDLTTDTTYQKEGTNLLS
                     LNKTVSYSSTYTAASGAWKADALVDGKISNTNPLGWTSDPNDPQQAEDAPVWVQIDLG
                     DVYGIERIVLWPRQDKDPVMGYPVDFTLQLSIDGDNWTTVATSTGNTNVDYDAKIFDI
                     SKTNARYVRMHCTKRAKDPGSNQWLAQLSEMAVYGGEPVTSSPETSDSAVSLLLISFV
                     IAFSACVLKVHKKKAEG"
     gene            1337..1582
                     /locus_tag="PLI11_01490"
     CDS             1337..1582
                     /locus_tag="PLI11_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51568.1"
                     /translation="MDITNLEKLAKIRLTEEERKEIEKDLAYFTTMLEDPFKADTSNV
                     QGTTIVSHTENVYRDDLVEDFEDLLFDGEIIVPRIVE"
     gene            1584..3014
                     /gene="gatA"
                     /locus_tag="PLI11_01495"
     CDS             1584..3014
                     /gene="gatA"
                     /locus_tag="PLI11_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPZ51569.1"
                     /translation="MPNRLSISECIKLLDNREIKVTELVNAYLREIENRDEEINAFIT
                     VTKDEALEKAEELDRKGKRISPLSGIPGGIKDNICTKNINTTCASKMLKDFVPKYSAT
                     VYERLLDNDFVMLGKLNMDEFAIGGGGEYSYFKPVKNPYNISHMAGGSSSGSAASVAA
                     FECAFSLGTDTGGSILKPSSYCGVVGMKPTFGTVSRYGVVGFAPSLEQTGPITRTVLD
                     NAIVLSKIAGHDEKDPCSIKRDLDFLQDIDKGLKGLKIAVPKELMDMDVIQRDVRKCY
                     EKSFEIFKELGCQVDVVSMPSLQYSLSAYYAISSSESASTLARYDGIRYGHRTEEYSN
                     IEELYEKTRGEGFGIDVKKRILFGTFLLVEKEKNYLDKARKARTILMNEYERLFKEYS
                     IVLTPASPITAPAFGEKQSNALEKYYEDIFTTPINLCGIPALSMPVCKDSNGLPVGII
                     LSARHYDEKTLYRFGHAFEAARGILK"
     gene            3011..4408
                     /gene="gatB"
                     /locus_tag="PLI11_01500"
     CDS             3011..4408
                     /gene="gatB"
                     /locus_tag="PLI11_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPZ51570.1"
                     /translation="MKYEAVIGLEVHVELKTNTKLFCGCRNEFGKKPNTLVCPVCAGM
                     PGTLPRLNKEAVNLAIKAGIGLNCKINKFSRFDRKNYYYPDLPKGYQTTQYEMPLCTD
                     GYIDIGGRKIGIERIHLEEDAGKLIHEEDKTFVDFNRSGVPLIEIVSRPDIRSGEEAA
                     LYLKKLRGILLFTGVSDCKMNEGSMRVDVNVSVRPKGLSFFGAKTEIKNINSFQYTQE
                     AIGYEINRHIELYENGKTPENETRRYDEKSKKTYTMRAKEDSSLYMYLPDTELVPLDI
                     SEEEIDEIRKKMPLLPEEREKMYVKEHGLSDFDCENILQEVHFAEYFEEAAKLSKDAK
                     NSANLLITQIFSLLESDKVIKIKPLRLAEVSNLMTDEVINSSTAKKLLEKLWEEDFDV
                     ENYVKENKLFQINEKDVLSRYIDEAMEENQKIVDDYRKGKKKALKALTGIVMAKTRGR
                     ANPRLLNTLLNEKLG"
     gene            complement(4376..5713)
                     /locus_tag="PLI11_01505"
     CDS             complement(4376..5713)
                     /locus_tag="PLI11_01505"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPZ51571.1"
                     /translation="MIKLSKETQLIIEKLNSCGFKAYIVGGCVRDYLMGLTPSDIDIT
                     TNAKPEETKEVFSDFRVIETGIKHGTVSVLVNGRQFEVTTFRIDSEYSDNRHPDSVSF
                     TSNIEEDLARRDFTMNAIAYNEQEGFIDPFGGMEDIKNRIIRCVGKPDKRFNEDALRI
                     LRALRFSSVTGFDIEENTKKALYGNKELLKNISVERIFQELIKLLCGDNVRKVLLEYV
                     DVLGVVIPELMPMKGFEQKNPYHAYDVLAHTAIVVESIEKKPYLRLAALFHDLGKPHT
                     FSLGEDNIGHFYGHAKLSTQMAIDILKRLKADNTTIAKVERFVRYHDTPIENDDVIIK
                     KRLNKFGKEDFFDLIKLMRADNRGQSPELSYRQKEYDLLEEKAHEILARQECFSLKDL
                     KINGHDIMALGVKEGREVGRILSALLGLVIANKVENSKEELVRSAKLLIEKGI"
     gene            complement(5834..6268)
                     /locus_tag="PLI11_01510"
     CDS             complement(5834..6268)
                     /locus_tag="PLI11_01510"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPZ51572.1"
                     /translation="MADTLFNAKATLKEGLQVECTARDFSIILDEPVSLGGTDTGMNP
                     VEALLSALGGCKCIVARAFAKKHKINLKNVEIELEGTLDPDGFLGRNPDAKIGFSRIV
                     TKFKIEADNTKEEIEKYVEFIESNCPVQDTLTNAAEFETVIV"
     gene            complement(6337..7269)
                     /locus_tag="PLI11_01515"
     CDS             complement(6337..7269)
                     /locus_tag="PLI11_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51573.1"
                     /translation="MPERLRLVNIFIATIVITLLISVASFGQEPLDLEIKYGTDKTVV
                     VKVLDDFSESGPPDKVWRDGFVEMRDGKIYCEGITIADCYLVEVPKANEHKSMYADAE
                     YIGFEIQNKCDGDIYYCFQGVIDGQDAFTSPEGEKNIVLVDKNGLLHEVEFSETAAIY
                     SRYGFIIPEGFDGYVFIPTERITRLGSWDTSIWHSDVTIEGVGVHVSTGQNPYPEVDA
                     SYVEFWVDNFFVFSGDFPEYEAPETPVPTNTPTPAPTNTPKDTPTPQKTAEATEKSND
                     TETDDKFPIVPVIIGAAVLVIVVVVVNIIAKKKK"
     gene            complement(7389..>7792)
                     /locus_tag="PLI11_01520"
     CDS             complement(7389..>7792)
                     /locus_tag="PLI11_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51574.1"
                     /translation="ESSTIINQMLTSLLSTYLTQQPDAGLLRCPDWILEAKEYIDENY
                     AEKVNVKEIAQMYHVSEAYFIRSFKKYFGKTPKTYIINKRLDEGKNLLLTSNDSINEI
                     AYKVGFPSHSLFTQFFKKMYGCTPSDFRNNS"
BASE COUNT         2473 a         1544 c         1470 g         2305 t
ORIGIN      
        1 ttctcgcaaa cattcattca tctcacaagg tgtattctat ttgacgctta cgtttattct
       61 aatatgtata cgcatgaata tgatgctttt tccttaataa aaggcggagg ttaaaagttt
      121 gccaattcaa atattaaaag aggaagtgac ataaagtttt atgtctgtta gtaatatacc
      181 aaaacacaca acaaaagttg catgtcataa aaaacctccg cctttgtgaa caaacaacat
      241 ctctttctct tatttgtcag tatattaata tttattggat aaaaaaacaa taggaataat
      301 aggttaagtc taaacaaaaa tatgtaaaaa caatcttaat gtatgccgtt tcaagattaa
      361 tgaatatatc catataatat gtgtttatct tgttatttga cagaggttaa atgtatttat
      421 tttacatatt tttatgccat atttacatat ttttatttgt gtcaaaacat gtaaaattgt
      481 taataataaa tactcattcg aggtgaaaat atgaaaaaga tcgtttttct ttcatttatt
      541 cttgtcattg cgctggcatc acagctttca gtaatggcag cgaataatat tgatttgact
      601 actgatacca cttatcaaaa agaaggcacc aatctgcttt ctttaaataa gactgtatcc
      661 tacagctcaa cttataccgc tgcatcagga gcatggaaag ccgatgccct tgtggacgga
      721 aaaatcagca ataccaatcc tttggggtgg acatcagacc cgaacgaccc tcaacaagcc
      781 gaagatgcac cagtgtgggt gcagattgat ttgggcgatg tgtatggcat tgaaagaata
      841 gttctctggc caagacagga caaggaccct gtaatgggat atcctgttga ctttacactt
      901 cagctatcaa ttgatggcga taattggaca actgtggcaa catcgacagg aaacaccaac
      961 gtcgattacg atgccaagat atttgatatc agtaaaacaa atgcaaggta tgtaagaatg
     1021 cactgcacca agcgtgcaaa agaccctgga tccaaccaat ggcttgcaca gctttctgaa
     1081 atggctgttt acggagggga gcctgtaact tcatctcctg aaacttccga tagtgctgtt
     1141 tctcttcttc ttatatcctt tgtaatagcg ttttctgctt gcgttttgaa agtacataaa
     1201 aagaaggctg aagggtaaaa tattatacaa aaagttttaa ttgcgcatcc ggttggatgc
     1261 gctttttttg ataaattttg cattaaaaca tatttatgat atatttttaa cgactaaggt
     1321 tttggaagtg ataattttgg atataacaaa tcttgagaaa cttgcaaaga taagattgac
     1381 tgaagaagaa agaaaagaaa ttgaaaaaga ccttgcatac tttacaacca tgctagaaga
     1441 ccctttcaaa gcagacacaa gcaatgtgca gggtacaacc attgtttccc atacagagaa
     1501 tgtgtatagg gatgatttgg tggaagattt tgaggatctg ctttttgatg gtgaaataat
     1561 tgtaccgagg atagtggagt agcatgccaa acagattaag tatttcagaa tgtattaaac
     1621 tacttgataa tagggaaata aaagtaaccg aacttgtgaa tgcatacctt cgtgagatcg
     1681 aaaatagaga cgaagaaata aatgcattta taacggttac aaaagacgaa gctttggaaa
     1741 aggccgagga actagacagg aaagggaaaa ggatttctcc cctttcaggc atccccggcg
     1801 gaattaaaga caacatatgt acgaaaaaca ttaacaccac ctgcgcatcc aaaatgctga
     1861 aggatttcgt accgaaatac agcgccaccg tgtatgaaag acttcttgat aatgattttg
     1921 tcatgcttgg aaaactcaac atggacgagt ttgccatagg aggcggcggt gaatattcat
     1981 acttcaaacc tgttaaaaac ccatataaca tatcccacat ggcaggaggt agctcctcag
     2041 gaagcgcagc ttccgtggct gcttttgaat gcgctttttc tttgggaacc gacactggcg
     2101 gatccattct aaaaccttct tcctactgcg gtgttgtggg catgaaacct accttcggaa
     2161 cagtatccag gtatggtgtt gttggctttg caccttccct tgaacagaca ggtcccataa
     2221 caagaacggt tttggacaac gcaattgttt tgagtaaaat tgcaggtcat gacgaaaagg
     2281 atccatgttc cataaaaaga gatttagact ttttgcaaga tattgacaaa ggattaaaag
     2341 gccttaaaat agcggttcca aaggaactta tggatatgga tgtaattcaa agggatgtaa
     2401 gaaagtgcta tgagaaatca tttgagatat tcaaagagct gggatgtcaa gtagatgtag
     2461 tatccatgcc cagtcttcaa tattcccttt ccgcatacta tgccatcagc agcagtgaaa
     2521 gcgcatcaac tttggcaaga tatgacggaa tcagatatgg tcacaggaca gaagaatact
     2581 caaacatcga agagctttat gaaaaaacca gaggagaggg atttggaatt gatgttaaga
     2641 aaagaattct gtttggaaca tttctgctgg ttgaaaagga aaagaattat cttgacaagg
     2701 caagaaaagc aaggacaatt ctaatgaacg aatacgaaag attgtttaag gagtattcca
     2761 ttgtccttac cccagcttca ccaataaccg cccctgcttt tggcgaaaag caaagcaacg
     2821 ctttggagaa atactacgaa gatatattta ccacaccaat taacttatgc ggaatacctg
     2881 ccttgtccat gcctgtgtgc aaagactcaa acggattgcc tgtcggtata attctaagcg
     2941 caaggcatta tgatgaaaag acactgtata gattcggaca tgcttttgag gcagcaaggg
     3001 ggattttaaa atgaagtatg aagcggtaat agggctagaa gtgcatgtag agttgaaaac
     3061 aaatacaaaa cttttctgcg gctgtagaaa tgagtttgga aaaaagccaa acacactcgt
     3121 atgcccggtt tgtgcaggca tgccggggac tttgccaaga ctgaacaagg aagctgtaaa
     3181 ccttgcaata aaagcgggga ttggcttgaa ctgcaagata aacaaattct ccagattcga
     3241 taggaaaaac tactattatc ccgatttgcc aaagggatat cagaccaccc agtatgaaat
     3301 gcctctttgc acagatggct atattgatat tggaggcaga aaaataggga tagaacgaat
     3361 tcatctggaa gaggatgcag gaaagctaat ccatgaagag gacaagacat ttgtggactt
     3421 taacagaagc ggtgtgccac ttattgaaat tgtgtcaagg ccggatatta gaagcggaga
     3481 ggaagccgcc ttgtatttga agaaactaag aggcatcctt cttttcacag gtgtatcaga
     3541 ctgcaaaatg aatgaaggct ccatgagggt ggatgtaaat gtttccgtaa ggccaaaagg
     3601 cttatctttc tttggtgcaa agacggagat aaagaatatt aattcttttc agtacactca
     3661 ggaagcaatt gggtatgaaa taaacaggca tatagagctt tacgagaacg gaaaaacacc
     3721 tgaaaacgaa acaaggagat atgacgaaaa aagtaaaaag acatacacca tgcgtgcaaa
     3781 ggaagattca agtttgtaca tgtatcttcc tgacacggaa cttgtgcccc ttgacatatc
     3841 tgaagaggaa atagatgaaa taagaaagaa aatgcctctt ctccctgagg aaagggagaa
     3901 aatgtatgta aaagagcatg gactttccga ctttgattgt gagaatattc tgcaggaggt
     3961 gcattttgca gagtactttg aggaagctgc aaaactgtca aaagatgcaa agaactctgc
     4021 aaatctttta atcacacaaa tattttctct tcttgaaagc gacaaagtaa ttaaaattaa
     4081 acctttgaga cttgctgaag tatcaaacct catgacggat gaggtcataa acagcagtac
     4141 cgctaaaaag ctacttgaaa agctctggga agaagacttt gatgtggaaa attacgttaa
     4201 ggaaaacaag ttgtttcaga ttaacgaaaa agatgtgtta tcaagataca ttgacgaggc
     4261 aatggaagaa aaccaaaaga tagttgacga ttacagaaag ggcaaaaaga aggccttaaa
     4321 agccctgact ggtattgtca tggcaaaaac aagaggcagg gcaaatccaa ggcttttaaa
     4381 tacccttctc aatgagaagc ttggctgatc ttacaagctc ctcttttgag ttttccactt
     4441 tgtttgcaat gacaagtcca agcaaagcgg aaaggattct tcctacttcc cttccctcct
     4501 ttacccccaa cgccataatg tcatgtccat ttattttcaa atccttcaag gaaaagcact
     4561 cctgcctggc aagtatctca tgagcctttt cctccaaaag atcatattcc ttctgcctgt
     4621 atgaaagctc aggactttga cccctgttgt ccgcccgcat aagttttatt aagtcaaaga
     4681 aatcttcttt cccaaacttg ttaagtcttt ttttgataat cacatcatcg ttttcaatgg
     4741 gcgtgtcgtg gtatctgaca aacctttcca cctttgcaat cgtagtgttg tccgccttca
     4801 gccttttgag tatgtcaatt gccatctgtg ttgaaagttt ggcatgacca tagaaatgcc
     4861 cgatattgtc ctcccccagg gaaaaggtat ggggttttcc caaatcatga aaaagagccg
     4921 ccagtcttag atatggtttt ttctctatgc tctccactac tattgcagtg tgggcaagca
     4981 catcataggc gtgatatgga tttttttgct caaatccttt cataggcata agttcaggaa
     5041 taaccacacc caaaacatcc acatattcaa gaagcacttt tcttacattg tctccgcata
     5101 acagcttgat tagttcctga aatattcttt caacagatat attctttaaa agctccttgt
     5161 tcccatataa tgcttttttt gtattctcct ctatatcaaa ccctgtaaca gaagaaaacc
     5221 tcaaagctct cagaattctt aatgcatctt cattaaacct tttgtccggc tttcccacac
     5281 acctgattat cctgttttta atatcctcca taccgccaaa aggatcaata aaaccttcct
     5341 gttcgttata cgcaatggca ttcatggtaa agtcccgcct tgccagatcc tcttcaatat
     5401 ttgatgtaaa ggaaacagag tcagggtgcc tgttgtccga atattccgaa tctattctaa
     5461 acgtagttac ctcaaattgc ctgccattaa ccaatacaga caccgttccg tgctttattc
     5521 ctgtctcaat tactctgaaa tccgagaaca cttcctttgt ttcctcaggc tttgcatttg
     5581 ttgtaatgtc aatgtcggaa ggagtcaaac ccattaaata atcccttaca catccaccta
     5641 cgatatatgc tttgaaacca cagctattca gtttttcaat aatcaattgt gtctctttgc
     5701 ttaacttaat cataattcac cattcttcca atacatttat ctctataatt aaattatacc
     5761 attactgctg cataaaaaaa gaaaaagccg gagatttatt ctccagcctc atatacttaa
     5821 ccatttgtat ttattataca atcaccgtct caaactccgc tgcgtttgtc aaagtatctt
     5881 gaacaggaca gttgctttca atgaattcaa cgtatttctc tatctcttcc ttcgtattgt
     5941 ctgcttcaat cttaaacttc gttacaatac gagaaaaacc aatctttgca tcaggattcc
     6001 ttccaagaaa accatccgga tccaacgttc cttctagctc aatttcaaca ttcttgaggt
     6061 ttatcttatg ctttttcgca aatgctcttg caacgatgca cttgcatcca cctaaagcag
     6121 acagaagagc ttcaacaggg ttcatgccag tgtcggttcc acccaatgat accggctcat
     6181 caagaataat gctaaagtcc ctagcagtgc attctacctg cagaccttcc ttgagtgttg
     6241 cttttgcatt aaatagtgta tcagccataa ttaccaatcc tttcttattt atgcattcag
     6301 tttaccacaa tctgtttctt tgtcaaaaaa cttatgtcat ttctttttct tagctattat
     6361 gttaaccaca accacaacta tcacaagaac cgctgcacca ataattactg gaacaattgg
     6421 aaacttgtca tcagtttcag tgtcgttgga cttttctgta gcctcggctg ttttttgtgg
     6481 tgttggcgta tcctttggag tattggttgg agccggagta ggagtatttg ttggcactgg
     6541 agtttcagga gcttcgtatt cagggaaatc accactgaaa acaaagaaat tgtcaaccca
     6601 aaattctaca tatgatgcat ccacctcagg atatggattc tgtcctgtac ttacatgaac
     6661 acccacacct tcgatagtta cgtcagaatg ccatatcgaa gtatcccagg aacctagtct
     6721 ggtaattctt tctgttggaa taaagacata accgtcaaaa ccttcaggga ttatgaaacc
     6781 atagcgactg tatatggctg ctgtctctga aaattcaact tcatgtaaaa gaccgttttt
     6841 atcaacaaga acaatattct tttctccttc aggcgaggta aaagcatcct gtccgtctat
     6901 tactccttgg aagcagtagt atatgtcacc atcacacttg ttctgtatct caaatcctat
     6961 gtattccgcg tccgcataca tgcttttatg ctcatttgcc ttgggaactt ccaccagata
     7021 acagtctgca atcgttattc cttcgcagta gattttgccg tctctcatct ctacaaaacc
     7081 atctctccaa accttgtcag gcggacccga ttcagagaag tcatcaagta cttttacaac
     7141 caccgtttta tctgttccat acttgatttc caaatcgagc ggttcctgcc caaaggatgc
     7201 aacagatatc aataaggtta taacaatggt tgctataaaa atattaacca aacgcaatct
     7261 ttctggcatt gcttttcctc ctcatataaa attgtacgta tttattatcc ttattgatat
     7321 tctaatttat ttgtgtcagt cagactatat ataaattgac gttaattata tttaaatcaa
     7381 catcggagct atgagttgtt tctgaaatcc gaaggagtac atccatacat ctttttgaag
     7441 aactgggtaa acagactgtg agaaggaaaa cccaccttgt atgcaatctc attaattgag
     7501 tcattggaag tcaataaaag attctttcct tcatcaagtc ttttattgat tatgtatgtt
     7561 ttaggcgtct ttccaaaata ctttttgaaa ctgcggataa agtacgcctc acttacatga
     7621 tacatctggg caatttcttt aacattaacc ttctccgcat agttttcatc aatatattcc
     7681 tttgcttcaa gaatccagtc agggcatctg agcagtcctg catctggctg ctgggtaaga
     7741 taagtggaca aaagacttgt aagcatctga tttatgatag tgcttgattc aa
//
LOCUS       DAONVY010000030         7770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7120_length_7770_cov_3.679715, whole genome shotgun sequence.
ACCESSION   DAONVY010000030 DAONVY010000000
VERSION     DAONVY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7120_length_7770_cov_3.679715"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLI11_01525"
     CDS             <1..502
                     /locus_tag="PLI11_01525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPZ51575.1"
                     /translation="DPYFLGFTIKSKYKASAKSFLKRDAGEKAGVFCYKDGRPYVIEY
                     TEIPKEYAELKDEKGDYVYGDTNVLNYIFDIDVAEDIASVGLPYHTQIKSVTAYINGE
                     YVESKKPDSYKFETFLFDSFSMLDDMGIFRIEREEEFAPVKNKTGIDSAETAREMYLA
                     VYGEDS"
     gene            complement(484..2415)
                     /locus_tag="PLI11_01530"
     CDS             complement(484..2415)
                     /locus_tag="PLI11_01530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPZ51576.1"
                     /translation="MYIRRSNLNVAVQYLFVITFILAFVFVPNKEENIKATNLVLVLD
                     PGHGGSDPGAVYYYDGIPIKEAELDMKIALAVRQELLKYEGVSVFLTREDNSKYLTLD
                     ERVNIASSLNADAIISIHNNASTNQQAHGAEVIVPSGNDGSGLHLISKALGDSILDEL
                     TNLGIHRRGLMQRISSTYKYKNGKPADYYGIIRHSMNNSLPGIIVECAFLSNENDYRT
                     FLNTDEKLEKIGQAVGRGIANYYGLIEGDNQMNSLGDLNQDTFISAYDAYLLNKQIGL
                     GQVGDAAISDVNSDGVVNILDVKEILEFSAGLRNDFSGGVTRAILKPGQVAAGIIMTQ
                     EEDPYLNVRTGPGVSYPAIGAYNKGTYIYITSGLGDKWCYARGICVFSGRIIEGYSSM
                     AYIDVIEKEVEDTDFEVQISGPSISLSNDEVTSGCIAIQFNSDHIEIESIETDMEYVT
                     TFGESGVLKIYFINSSNLSDTINICFASMDSSVDKADVTLSFAELTDIEGKELTTEPE
                     TITLTFAEPMPFDLEIKDSYVYGFECGFTVGEILEKCKVVEEVFLENGENAQEDTLLA
                     TGMKVSMDASVYIVVIFGDVNGDGIVDICDIESIQKHLLEASVLDGAYYNAADINCDS
                     AINALDIGACQKFIYESSP"
     gene            2641..3546
                     /locus_tag="PLI11_01535"
     CDS             2641..3546
                     /locus_tag="PLI11_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51577.1"
                     /translation="MRKLLTVLMVLSLILSMAVMSVHAEAPAYKAKAGAATIDGKISE
                     GEYGTKYEFNKDNTVAFSSSYDVDPLPFSFYINWTEEGLYIAYELKVEEGDWGDNKLF
                     PQFDMNPWNVLGDDQEGLFFSFFFDAKDKIRFTIHNHGTVGNSEASGSQADLEDIDYA
                     IEVEDDGSTVLEFMIPVKYLQVKDGDIDASDKKLEAGQTWGLSAFLIRDNKGWAQIGA
                     PDNFVSDVENPFHIGSLKLGTITLEEETTPEPEETPAPTDKDEDKDDDTDKGGVGAPV
                     IAAIVIGVIIVAAVVVYFVTRKKDQ"
     gene            3684..4619
                     /locus_tag="PLI11_01540"
     CDS             3684..4619
                     /locus_tag="PLI11_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPZ51578.1"
                     /translation="MTLEVKNVYKSFGTKGVLKDVSFSASSGSALGLLGRNGAGKTTI
                     MRIIMRFFNADEGSILLDNKTFYPENYSIGYLPEERGLYPKKPIGSQLIYLARLKGLS
                     TKDAKANTVKWLERLGLEDQFSKKLNTLSKGNQQKIQLAASLVNDPDIVILDEPFSGL
                     DPVNAVLLKNIVKELVDDGKIVIFSSHQMNYVEEFCDRIALLNRGTIVLHDSIFNIKK
                     SYPNDNVFVSFTKPQNIESIKNFVDDHVMSFEAQKAGANTEKEAYSFIMKLKDDVSKD
                     AMLRMFVDANYEIDDFHVIEPTLEEIFVDKAGDAI"
     gene            4616..5884
                     /locus_tag="PLI11_01545"
     CDS             4616..5884
                     /locus_tag="PLI11_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ51579.1"
                     /translation="MIKKRDWEQFKTIFSYEYMGILKNKVYLITTIIIMVVIAVVLTT
                     PTVIGMFKSEDIPGDEPGTDPVGTDKLKVAVINNSGVHIEEVLNLSLPNYDFIAEDSN
                     LDELKDKVNKEEYYAALVMSKPLEFDLYVKDYVITNTLTQQISSVIEYNYKITELQNQ
                     GVSLEEIQRILASTVKSNLIELGKSSEQTFLYAYILLMFLYIAVNLYGQLVATSVATE
                     KSSRAMELLISSAKPLNFMFGKILGSGTAGLTQLVAIMGVSFGFYKINESTWSKYPLV
                     TSLFDMPADVLFYTILFFLLGFFLYAFIYGAVGSLVSRIEDVNTSILPVTFLYMAGFL
                     VSMVNLVNPKGIVVRVFSFIPFFSPMCMFLRIIMTSVPMWEIALSIAILIATILLTGY
                     IAAKIYRVGVLMYGKPPKIKELIKVLRTKE"
     gene            5889..6818
                     /locus_tag="PLI11_01550"
     CDS             5889..6818
                     /locus_tag="PLI11_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51580.1"
                     /translation="MEYAIEINNVTKKFGDVTAVDNLSVKLPKGTICGFVGPNGAGKT
                     TTIKMLMGLIKPTSGELFVNGVKVKSGLERKLHVGYLPDVPGFYDWMTAEQYLHFCGK
                     LLQMDKEDIRKRTAELLERFGLSGVKTKIKGYSRGMKQRLGIAQALIGDPSVVLLDEP
                     ASALDPIGRKAIFDLILSLKGKVTVLFSTHIIADVERVCDTVLIMDKGVLKVNTEINE
                     LTAKQAEHIINMSVKGEDESINTLVSTLENKEYIKSVGRNQNGIIQIVALDDMLAGRE
                     IPALINEHSLALMNYNVQTRRLESIFLEVVSHE"
     gene            6811..7566
                     /locus_tag="PLI11_01555"
     CDS             6811..7566
                     /locus_tag="PLI11_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPZ51581.1"
                     /translation="MNNFIVFLKKELKEFVKTWKLLILLALFIVNSFMSPILYYYMPK
                     LLELSGLPGAAEQFDMSLASYYTQYYGNQFQSGFLALIFITCLAVVREKKKGTAILTL
                     TKNISRPTFVLAKYISYVIWYTVVYVVSSAAFAAMIKVLINESINKHTIASFGLFYLI
                     GIMFAAGAIFASCIGKNTIVSCLIGFGVYIAVFILAAIPYVNKFTPAMLLDNSITALS
                     GNYENLLIPLLTCIGCTVLFLVLGIVSFEKQEL"
BASE COUNT         2355 a         1479 c         1577 g         2359 t
ORIGIN      
        1 ggatccatat tttctcggat tcacaattaa aagcaaatac aaggcatcgg caaaatcatt
       61 cttgaaaaga gatgcaggcg aaaaggcagg ggtattttgt tacaaagacg gcagaccata
      121 cgttattgag tataccgaga tacctaagga atatgccgaa ctcaaggatg agaaaggtga
      181 ttatgtgtat ggtgacacga atgtgttaaa ttacattttt gacattgacg ttgcagaaga
      241 catagcaagt gtaggtcttc cctaccacac acaaataaaa tccgttactg cctacattaa
      301 tggagaatat gttgagtcga aaaaacctga cagttacaag ttcgaaacat tcttattcga
      361 ttctttcagc atgctggatg atatgggcat tttcagaatc gaaagagagg aagagtttgc
      421 accagtgaaa aacaaaacag gcattgactc tgcagaaacc gcaagagaaa tgtatcttgc
      481 agtttacggt gaggattcgt agatgaactt ttgacatgca cctatgtcca aggcattgat
      541 agcactgtcg caatttatat cagctgcatt gtaatacgct ccatcaagaa cacttgcttc
      601 aagcaggtgt ttttgaatgc tttcaatgtc gcatatgtct actattccat caccatttac
      661 atctccaaaa attactacaa tataaacaga ggcgtccata gaaactttca tcccggtagc
      721 aagcaacgta tcctcttgtg cattctcacc gttttccaga aatacttctt ctacaacttt
      781 gcatttttca agtatttcac cgacagtaaa accacattca aagccataca catagctatc
      841 ttttatctct agatcaaagg gcatgggttc tgcaaaagtc agcgttattg tctctggctc
      901 tgtcgtcagc tctttccctt caatatcagt caattcagca aaacttaaag tcacatccgc
      961 cttatccaca cttgaatcca tgcttgcaaa gcatatgtta attgtatcgc ttaagttgct
     1021 tgaattaatg aaatatatct tcaaaacacc actttcgccg aatgttgtaa catattccat
     1081 atctgtttcg atactctcaa tctcaatatg gtccgagtta aactgtattg caatgcaacc
     1141 ggaggtaact tcatcattgc ttaatgaaat gctcggtcca cttatttgca cttcaaaatc
     1201 agtgtcttct acttcctttt caataacatc aatgtatgcc atgctgctgt agccttcaat
     1261 tattcttcct gagaaaacac atatgcctct tgcatagcac cacttgtcac ccagtccgct
     1321 ggtaatgtag atatatgtgc ctttgttgta tgctccgatt gcaggatagg aaacaccagg
     1381 accagtcctt acattcagat acggatcttc ctcctgggtc atgattattc ctgctgccac
     1441 ttgtccaggc tttaatatgg cccgggtaac accacctgag aaatcatttc taaggccagc
     1501 agagaactca agaatctcct ttacatcaag aatattcact actccgtcag agttcacgtc
     1561 tgatatggca gcatctccta cctggccaag acctatttgc ttgtttagca aatatgcatc
     1621 ataagcggaa atgaaggtgt cctgatttag atcgcccaat gagttcattt ggttgtctcc
     1681 ttctatgaga ccgtaataat tggcgatacc cctgcccact gcttgtccaa tcttttcaag
     1741 tttctcatcg gtattaagaa acgttctata gtcgttttca ttgctaagaa atgcacattc
     1801 gacaatgatt cctggaaggg agttattcat gctgtgcctt attatcccat agtaatcagc
     1861 aggttttcca tttttatatt tatatgtaga ggatattctt tgcatcagcc ctcttctatg
     1921 tattcccaga tttgtcaatt catcaagtat tgaatcaccc agggcttttg atattaagtg
     1981 aaggccggat ccgtcgttac ccgaggggac aattacctct gcaccatgag cttgctggtt
     2041 tgttgatgcg ttgttatgta tgcttattat ggcatcggca ttcaatgatg atgcgatatt
     2101 gacgcgttcg tccaatgtaa ggtatttgga gttgtcttct ctcgtcaaga atacggaaac
     2161 tccttcatat ttcaaaagct cctgtcttac tgccagtgca attttcatat ccaattcggc
     2221 ttctttaatg gggataccgt cgtagtagta tactgctccc gggtcgctac caccatgacc
     2281 tggatccaaa acaagcacca agttagtagc ctttatgttc tcttccttgt ttggaacaaa
     2341 cacaaaagcc agtataaaag ttataacgaa taaatattgg acagccacat tcaaattgct
     2401 tcgtcttatg tacatttggc agacaacctc ttccgcaaat attgctctaa accctgcttg
     2461 attcaagctg tttttagtat ataatggcta gtaactccat tgcttgaatt ttatcaattg
     2521 tttatttttt ctatatcata tcataatgtt tttttaagtt caataaatta tattttattt
     2581 gacatgtata tgtcgctgtg ttaatattag accgaatatt agttacagga ggtaaattaa
     2641 atgagaaaat tattgacagt tttaatggtt ttgtcattga ttctttcgat ggctgtaatg
     2701 tctgtgcatg ctgaagctcc agcatacaag gcaaaagcag gtgctgctac tattgatggc
     2761 aagatttctg aaggtgaata cggaacaaag tatgaattca acaaggacaa caccgtcgca
     2821 tttagttcat catacgatgt agacccactt ccattcagct tctacataaa ctggactgaa
     2881 gaaggtctgt atattgctta cgagcttaag gttgaagaag gcgactgggg tgacaacaag
     2941 ttattcccac agttcgacat gaatccttgg aatgttctgg gggacgacca ggaaggtttg
     3001 ttcttctcat tcttcttcga tgcaaaggat aaaataagat ttacaatcca caaccatggc
     3061 acagtgggca actcagaagc cagcggttcg caagcagatc ttgaagatat tgattatgcg
     3121 atcgaagtcg aagatgacgg ctctactgtt ctcgagttca tgatacccgt aaaatatctg
     3181 caagtaaaag atggagacat tgatgcttcc gataagaaac ttgaagctgg acaaacctgg
     3241 ggtctctccg cattccttat ccgtgacaac aagggatggg cacaaatcgg agctccagat
     3301 aattttgtat ctgatgtaga aaatccattc catatcggca gcttgaagct aggcactatc
     3361 actcttgagg aggaaactac cccagagcca gaagaaactc ctgctccaac cgacaaggac
     3421 gaagacaagg atgacgacac tgacaagggt ggcgttggcg caccagtaat tgccgcaatc
     3481 gttataggtg ttatcatcgt tgcagctgtt gttgtttact ttgtaacaag aaaaaaggac
     3541 caataaatac ctacctgaaa aaaatgtaat aaactcaacc gaaaggtagc ctgattggct
     3601 gcctttcttt ttgcttctat tatattatgg acaattttcc acttattgtg taaaatattc
     3661 taaaacacag aaaggagcct ctcatgacat tagaagttaa gaacgtatat aagagctttg
     3721 gaaccaaagg agtcttgaaa gatgtgagct tttctgcaag cagtgggtct gcattagggc
     3781 tactaggcag gaacggagcc gggaaaacca ccattatgag aattataatg aggttcttca
     3841 acgctgacga aggcagtatc cttcttgaca acaagacatt ttatccagag aattacagca
     3901 ttggctatct gccggaggaa agaggtcttt accccaaaaa gccaattgga agccagctta
     3961 tttatctggc aagactaaaa ggtctcagta cgaaggatgc aaaggctaat acagtaaagt
     4021 ggcttgaacg acttggtttg gaggatcagt ttagcaaaaa gttaaatacc ctttcaaagg
     4081 gaaaccagca gaaaatacag ctggcagcta gtcttgttaa tgatccggat attgtgattc
     4141 ttgatgagcc ttttagtgga cttgatccgg taaatgccgt attgctaaaa aacattgtta
     4201 aagagcttgt tgatgacggc aaaattgtga tcttctcaag ccatcagatg aattatgtag
     4261 aggaattctg tgacaggata gctcttttga accgtggtac gattgtattg cacgacagca
     4321 ttttcaatat taagaagagt tatccgaacg acaatgtttt tgtatccttt accaaacctc
     4381 aaaatattga gagtattaag aactttgtag acgaccatgt gatgtcattc gaggctcaaa
     4441 aagctggcgc aaatacagag aaggaagctt attcatttat tatgaagcta aaggacgatg
     4501 tctccaagga tgcaatgctt agaatgtttg ttgatgcaaa ctacgaaata gatgattttc
     4561 atgttattga gcccaccttg gaggagatat ttgtggataa ggcaggtgac gccatatgat
     4621 aaaaaaacgg gattgggaac agttcaagac catattttcc tatgagtata tgggaatact
     4681 taagaataaa gtgtatttaa tcaccacaat cataatcatg gtggttatag cggttgtttt
     4741 aacaactccg acagttattg gtatgttcaa aagcgaagac atacccggcg atgagcctgg
     4801 tacggatcct gttggtactg ataaattgaa ggttgctgtt attaataatt caggggtgca
     4861 tatagaagaa gttctgaact tgagcctgcc taattatgac tttattgccg aagacagcaa
     4921 tctggatgaa cttaaagaca aggttaacaa agaggaatat tacgcagctt tggttatgtc
     4981 aaaaccactt gagtttgacc tttatgtaaa ggattatgtg attaccaaca ccttgacgca
     5041 gcagatttca agcgttattg agtataatta caagataacc gaactgcaga atcaaggagt
     5101 gtccttggaa gaaatccaaa gaattctggc cagtaccgta aagtccaatt tgattgaact
     5161 tggaaaaagc agtgagcaaa cttttcttta cgcatacatc ctgcttatgt tcctgtatat
     5221 tgcggtgaat ctttacggac agcttgtagc tacttctgtt gccaccgaga agagctcaag
     5281 ggcaatggag ctgctaatat catcagccaa acctctcaac ttcatgtttg gcaagattct
     5341 tggctcaggt actgcaggtc ttacgcaact ggttgccata atgggtgttt cctttggatt
     5401 ctataaaata aatgagagta catggagcaa gtacccattg gtaacatcat tgtttgacat
     5461 gcccgctgat gtactgtttt acacaatact gtttttcctg ctcggcttct tcctgtatgc
     5521 ctttatctat ggcgctgtag gctcgcttgt aagcaggatt gaagatgtta acacaagtat
     5581 tttgcctgta acattcctgt atatggcagg ttttcttgtg agtatggtaa accttgttaa
     5641 tccaaaaggc atcgttgtca gagtattctc tttcatacct ttcttctcac ctatgtgtat
     5701 gtttcttaga attattatga catcggtacc gatgtgggaa atagctcttt caattgccat
     5761 cctaattgca acaattcttc ttacaggtta tattgctgct aaaatttaca gggttggtgt
     5821 cttaatgtac ggcaagccac caaagataaa agaacttatc aaagttttaa gaacaaagga
     5881 gtgagtttat ggaatatgca attgagataa acaacgtgac aaaaaaattt ggcgatgtga
     5941 ctgctgttga caacttgtca gtaaagttgc caaaaggaac gatttgcggt tttgtaggac
     6001 caaacggtgc tggcaaaact acaacaataa aaatgctaat gggacttatc aagcctacgt
     6061 caggtgagct ttttgtaaat ggagtgaagg ttaaaagcgg acttgaaaga aagctgcatg
     6121 taggatattt gcctgacgtg ccagggtttt atgattggat gacagcagaa cagtatctac
     6181 atttttgcgg caaactgtta caaatggaca aagaagacat aaggaaaaga actgctgaat
     6241 tacttgaacg ttttgggctg agtggtgtta agacaaagat aaagggttat tcaagaggta
     6301 tgaagcaacg gcttggtatt gcccaggctc ttattggaga tccatccgtt gtgctgcttg
     6361 acgaacccgc atccgcattg gaccctatag gaagaaaagc gatttttgat ttgattctat
     6421 cattgaaggg taaagttact gttttattct caacacatat catagcagac gttgaaaggg
     6481 tttgtgatac tgttttaata atggataaag gagtgctgaa agtaaacaca gaaataaacg
     6541 aactgactgc gaaacaagca gagcatatta taaacatgtc agtcaaggga gaagatgaat
     6601 ctattaatac actcgtctcc acccttgaaa acaaagagta tataaagtct gtagggagaa
     6661 atcagaacgg aattattcag attgttgcat tggacgacat gttggcaggc cgtgaaatac
     6721 cagcattaat caatgagcac tcattggcgc ttatgaatta caacgtgcag acaagaagac
     6781 ttgaatcaat attcctggag gttgtgtcac atgaataact ttattgtttt cctgaaaaaa
     6841 gaactgaagg aattcgtaaa aacatggaag ctattgatat tgcttgccct gtttattgtc
     6901 aacagtttta tgtcccctat tctttactac tatatgccaa agctccttga gttgtctggt
     6961 ttgcctggtg ctgctgaaca gtttgacatg tcattagcgt catactatac acagtactac
     7021 ggaaatcaat ttcaatcagg gtttctggca ttaattttca ttacgtgcct tgcagttgta
     7081 agggaaaaga agaaagggac tgcgattctt acactgacca agaacatatc aagaccaact
     7141 tttgttcttg caaaatacat ctcatatgta atttggtaca ctgttgtata tgttgtttcc
     7201 tctgcagcct ttgctgcaat gattaaagtt ttgattaacg aaagcataaa caaacataca
     7261 atagcatcat ttggtttgtt ttatcttata ggtattatgt ttgccgccgg agcaattttt
     7321 gcaagttgta ttggaaaaaa cactattgtc tcctgtttga ttggttttgg agtatatata
     7381 gccgtattta ttctggctgc cataccatat gtaaacaagt ttacaccagc aatgcttttg
     7441 gacaattcaa ttacggcact ctccggaaat tatgaaaatc ttctcatccc tctgcttaca
     7501 tgtattggat gtactgtatt atttcttgtc cttggtattg tctcctttga aaaacaggaa
     7561 ctgtaaacct acagacaatc cagatgcaac accatggtaa ccatggtgtt gctttttttg
     7621 tattagaaaa tttacaacct agtaaggaca gtattttaaa aaaagttatt gacacaagct
     7681 tattttaggt gtataattta ttttgctgtt tgaacagcac atgcaccttt agctcagatg
     7741 gtagagcaac tgactcttaa tcagtgggtc
//
LOCUS       DAONVY010000031         7746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7148_length_7746_cov_3.746717, whole genome shotgun sequence.
ACCESSION   DAONVY010000031 DAONVY010000000
VERSION     DAONVY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7148_length_7746_cov_3.746717"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLI11_01560"
     CDS             <1..412
                     /locus_tag="PLI11_01560"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ51582.1"
                     /translation="PEKISESLKKEFSKEYGSWETEEIYGMYDDIPEELKKLYEKYSS
                     WEFRYGETPSFDMAFENRFTWGGIDINLSVEKGVITKAYVYSDAMDTAVAETLADLLP
                     GIPLDRDEILDKLNEAGINLTELKDVKNWLAEVL"
     gene            complement(409..906)
                     /locus_tag="PLI11_01565"
     CDS             complement(409..906)
                     /locus_tag="PLI11_01565"
                     /inference="COORDINATES: protein motif:HMM:NF004088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPZ51583.1"
                     /translation="MSLLEKWKDNLQNLRGNALNEYIKGYYKKEENAYARILGEKNPE
                     ISGIVSELAEKFGMEDYEFAAFMDGIAESVDPSIDAEQLGPETEVCATVDWARLYKNM
                     HKAKADWLYNLPEWEGIFDEEKRRDLLFEYRREGQAVRENTVGRNDPCTCGSGKKYKK
                     CCGKS"
     gene            complement(908..1708)
                     /locus_tag="PLI11_01570"
     CDS             complement(908..1708)
                     /locus_tag="PLI11_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPZ51584.1"
                     /translation="MITSDQNTKIKFVRSLSKRKFREKHNCFYIEGHRAVTEGILLGK
                     RPQMLLVSESYTKQDSYVELLDICFAQGIDAKQVLLIANDKVFSQLSDTKTPQGIGGV
                     FQIPVINKSALSNMRNIVLLENLQDPGNMGTIIRTADAAGFDAVVCSKGTVDVYNSKV
                     LRSTVGSIFRIDIVMLDEDTKDFAVKLKERGFKLYCAHPRGDLSSFETEFGNHNCIVI
                     GNEANGVSDKMLSVCDYKIYIPMPGRAESLNASVAAALMIYEVVRKSD"
     gene            complement(1710..2060)
                     /gene="rplT"
                     /locus_tag="PLI11_01575"
     CDS             complement(1710..2060)
                     /gene="rplT"
                     /locus_tag="PLI11_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPZ51585.1"
                     /translation="MARVKNSVQTRARRKKVLKLAKGYFGRKSKVFRTANQAVIKSAN
                     YAYRDRRRKKRDFRRLWITRINAAARMNGMSYSKFMHGLKEKNIHLNRKVLADMAVND
                     MEAFAKLVETVKAK"
     gene            complement(2076..2273)
                     /gene="rpmI"
                     /locus_tag="PLI11_01580"
     CDS             complement(2076..2273)
                     /gene="rpmI"
                     /locus_tag="PLI11_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPZ51586.1"
                     /translation="MPKLKTHSSSKKRFKISAGGKVKMSHANRRHRLVSKSKSAKKRH
                     KMPTFAAKSNSAVVKKLLPYS"
     gene            complement(2332..2823)
                     /gene="infC"
                     /locus_tag="PLI11_01585"
     CDS             complement(2332..2823)
                     /gene="infC"
                     /locus_tag="PLI11_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPZ51587.1"
                     /translation="MINEEIRDREVRLVDETGKALGIVSSKEALKIAYEKNLDLVKIA
                     PNANPPVCKIMDYGKYMFDLAKKEKEVRKNQKIVNVKEVRIGLDIGEHDFNVKVKQAI
                     RFLKSEDKVKLTVRFVGREMNFTDRGHELINRFVECVSEVGTMEKKPKLEGRRMSVII
                     NPK"
     misc_feature    complement(2852..2990)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3089..3517
                     /locus_tag="PLI11_01590"
     CDS             3089..3517
                     /locus_tag="PLI11_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51588.1"
                     /translation="MKKDADLLNNIYQNSQMGVNTMKQLIEITEDENFKKQLEEQYKE
                     YKEIRKLAEQKLNERGYDEKGINTFDKIKTYLMINFQTLTDKSTSHIAEMLMIGSMMG
                     IINAVRNLKEHKDCENDILDLMERLLKFEEHNIQKLKEYL"
     gene            complement(3680..4525)
                     /locus_tag="PLI11_01595"
     CDS             complement(3680..4525)
                     /locus_tag="PLI11_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51589.1"
                     /translation="MCLVLLFAASACEEAEVEVEESYLTVYNCRVHDKDLHPDKVVLD
                     KLSGKRMEYEINGVTYELQYEYTRPKGVSFGYDVDVYSVLNAEPYFNGSINKPMIFFV
                     SGTDKLHRIHNLKVTEARSIKNKQDLAEIANEFLSKYVDLGDFKVDEVNMPRRNANSF
                     SDIGVYSYSMEFDGYKAGATMPEVSVTAQGEIVGVFLPNMEYLQEFKNREFNIDVKEC
                     EKAMVKSISDYFKTECWIKVENIYVLISGDGKSCAYFNTTVFSKVRGNNLSEPMPFVV
                     ELEQS"
     gene            complement(4640..6379)
                     /locus_tag="PLI11_01600"
     CDS             complement(4640..6379)
                     /locus_tag="PLI11_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51590.1"
                     /translation="MKKKKHTVIRLLQYMKDFKWHLVLALVLTIIGNAFNLIGPMMSG
                     KAISYIEPGTGSVNFQKVFQIAVLMLVVYLLSSILTYLLSLLMVYISRKVVYNMRKDV
                     FEKITTLPVGFFDVNPTGDIISRMSYDIDTINTSLSSDLVQISASFITVIGSLVMMLV
                     ISPYLVLVFAFTVPTSIFLTRFITKRTSPLFKARSARLGEMNGFVEEMVTGQKTLRAY
                     NSEDSTIDKFVDYNEKTATAYYKSEYYGSMTGPSINFVNNLSLSLISVFGAIMYLYRT
                     ISLGDIASFVLYSRKFSGPINEMANIATELQSALAAAERVFRLLDEEPEKEDSKDAVV
                     LDGVEGDVKLEDVNFGYTKDKLVIKNLNLIAPKGSLVAIVGKTGAGKTTLINLLMRFY
                     DVDDGIILVDGVPVEDITRASLRKSYAMVLQDTWLFSGTVFENIAYGKENASREEVEE
                     AAKAAMIHSFIEKLPKGYDTVLSDDAANISKGQKQLLTIARAMLIDAKILILDEATSN
                     VDTNTEIKIQKAMRNLMKDKTCFVIAHRLSTIRNSDVILVVDEGNIVEMGTHEDLLKK
                     KGMYYEMYYSQFE"
     gene            complement(6376..>7746)
                     /locus_tag="PLI11_01605"
     CDS             complement(6376..>7746)
                     /locus_tag="PLI11_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51591.1"
                     /translation="RMTSDTYNVHRMVGMMQRIGIRAPILLIGGLLVTLTLDPVLTLV
                     LVVVLVFLVFITYTVSSKGIPLFRTVQESTEDMIRVLRENITGVRVIKALSKSDYEKD
                     RFDKVNKKVMNDENKANMVLNTIKPAMNFLLQGGLVGVILLGAFRVNAGVSEVGKIVA
                     FLTYFTIILNAMLSITRIFTVFSRSIASANRIQEVLEVKPSDTTSFENILEKDDEDFI
                     TFENVSFSYNKVKDDVSDISFGIKKGETLGIIGATGSGKSTLLKLLMRFYDPDKGSIR
                     IDGKDVRSYDLKDLRRRFGVVFQNDALFRDTVAENIKLGREISIEDISRSIEHAQASD
                     FVKGVGLDFMLSIKGANFSGGQKQRLLIARALAGRPEILILDDASSALDYKTDASLRR
                     ELKKHYKGTTTIIIAQRISSIKHAERIIVLDEGRILGYGTHDELIKSNELYQEIASTQ
                     MGEMLV"
BASE COUNT         2236 a         1805 c         1280 g         2425 t
ORIGIN      
        1 tcctgaaaag atatccgaat cattaaaaaa ggaattttca aaagagtacg gttcttggga
       61 aaccgaagaa atatacggca tgtatgacga tatccccgag gaactaaaga aactgtacga
      121 gaagtactca tcatgggaat tcagatacgg tgaaacccct tcctttgaca tggcatttga
      181 aaacaggttc acatggggtg gaatagacat taacttgtcg gttgaaaaag gcgttataac
      241 gaaagcgtat gtttactctg atgcaatgga cacagctgtt gcagaaacac tagcggattt
      301 gcttcctgga atccctcttg acagggatga gatacttgat aaattaaatg aagcaggtat
      361 aaatcttact gaacttaaag atgtgaaaaa ctggcttgcg gaagtccttt aggacttacc
      421 gcaacatttt ttatacttct ttccactgcc gcaagtacag ggatcgtttc ttccaacagt
      481 attttctctt actgcctgtc cttcccttct gtactcaaac aaaagatcgc gtcttttttc
      541 ttcatcgaag atgccttccc attcaggtag attgtaaagc cagtcagcct ttgccttgtg
      601 catgttcttg tacagccttg cccagtcaac agttgcacaa acctctgttt caggtcccaa
      661 ctgctccgca tcaatggaag ggtcaactga ctcagcgatg ccgtccataa acgcagcaaa
      721 ctcgtaatct tccattccaa atttctctgc aagttcggaa actattcccg atatctcagg
      781 attcttttct ccgagaattc ttgcatatgc attttcctct tttttgtaat accctttaat
      841 atactcgttt agtgcattac ctcttaaatt ttgcaaatta tctttccact tttcaagcaa
      901 actcaattca atcactcttt cttacaactt catatatcat aagtgcggca gctaccgacg
      961 catttaacga ttccgctctt cccggcatgg gaatatatat cttgtaatcg caaactgaaa
     1021 gcattttatc gctaacacca tttgcctcgt ttccaatcac aatgcagttg tgatttccaa
     1081 actctgtttc aaaagaactc aagtctcccc tgggatgagc acagtacagt ttaaaacctc
     1141 tttctttaag tttcacagca aaatcttttg tatcctcatc cagcatgaca atatctatgc
     1201 gaaagatgga tcccactgtc gacctgagca ctttcgaatt gtacacatct accgtacctt
     1261 tagaacacac aaccgcatca aaacccgccg catctgctgt tctaataatt gtacccatgt
     1321 tgcccgggtc ctgcagattt tccaaaagca caatattcct catattggat aaagcacttt
     1381 tattaataac tggaatttga aaaactcccc caataccttg aggagttttc gtatcactca
     1441 actgtgaaaa caccttgtca tttgctatca ataaaacttg cttcgcatca attccttgtg
     1501 cgaagcaaat atccaaaagt tccacatatg agtcttgttt tgtgtaggat tccgaaacca
     1561 gcagcatttg cggtctttta cctaacagaa tgccttctgt tacggctctg tgaccttcga
     1621 tgtaaaagca gttgtgcttt tccctgaatt ttcgcttgct cagactcctt acaaatttaa
     1681 tcttcgtgtt ttgatcactg gtaatcatgt tactttgctt ttacagtctc aaccaacttt
     1741 gcaaaagctt ccatatcatt tacagccatg tcagcaagaa cttttctgtt caggtggatg
     1801 tttttctcct taagtccatg catgaacttg ctgtaggaca tgccgttcat tcttgcagcg
     1861 gcattgattc ttgtaatcca gagtcttctg aagtctctct tcttgcgtct tctatctcta
     1921 tatgcataat tagcagattt gataacagcc tggtttgcgg ttctgaacac tttgctcttt
     1981 ctgccaaaat aaccttttgc cagcttcagg acttttttac gtcttgctct tgtttgtact
     2041 gaattcttaa ctcttgccat tttcacattc ctcccttacg aatatggaag cagtttctta
     2101 acaactgctg aatttgattt tgctgcaaaa gttggcatct tgtgtctttt ctttgcactc
     2161 tttgacttac ttaccagtct gtgacgtcta ttcgcatgac tcatttttac tttaccacca
     2221 gcagagatct tgaatctctt cttagaagaa ctgtgggttt tcaattttgg cataataatt
     2281 cctccatatc gcttcataat attataataa caacacttgt tgggtaatct gctattttgg
     2341 attgatgata acagacatgc gtcttccttc gagttttggt ttcttttcca tagtccctac
     2401 ttcagacacg cactcaacaa agcggttgat taattcgtgt cctctgtctg taaaattcat
     2461 ttctcgtcca acaaacctaa cggtcagctt gaccttgtcc tcactcttca ggaaccggat
     2521 tgcttgtttg accttaacat taaaatcatg ttcgcctatg tcaagaccaa ttctaacctc
     2581 tttgacattg acaatctttt gattctttct gacctctttt tccttcttgg ccaaatcgaa
     2641 catatacttt ccatagtcca ttatcttgca cactggcgga ttcgcattcg gtgcaatttt
     2701 gaccaaatcc aggtttttct cataggcaat tttcagcgct tccttcgaag aaacaattcc
     2761 aagggctttt ccggtctcat cgacaagcct aacttccctg tctctgattt cttcgtttat
     2821 catcagttca ttcttgctaa tagcagacac ctccaaaaag atatagcgga ttagaataaa
     2881 gtctaatccg cttgaactca aacaaaacaa gatacattta atatcctttg tcagtaacct
     2941 gggctgccaa tggcaccaag gtgagaagcg gaagacttct tcttaaatcc tttagtattc
     3001 tatcatcaat atgcaattgt gtcaaacact ttttttgcat aaaattatgc aaaatcattc
     3061 atactattaa aaaatcccgg aggaaatcat gaaaaaagac gcagaccttc taaacaatat
     3121 ataccaaaac tcccagatgg gagtcaatac gatgaaacag ctaattgaaa taacagagga
     3181 tgaaaatttc aaaaagcagc tggaagagca gtacaaggaa tacaaagaga taagaaagct
     3241 tgcagagcaa aaattaaatg aaagaggata tgacgaaaaa ggaatcaata ccttcgacaa
     3301 gattaagaca taccttatga taaactttca aacattaacc gacaaatcca catcccatat
     3361 tgcagagatg cttatgattg gcagcatgat gggaatcatt aatgctgtaa ggaatctaaa
     3421 ggagcataaa gattgtgaaa acgacatcct cgaccttatg gaacgtctat taaaattcga
     3481 agaacacaat atccaaaaac tgaaagaata cttgtaatcc tgaatgcatg acgcattcag
     3541 gatttaacct tcattactca tacacctttc aactcttccg tcctaactaa gttttctaca
     3601 gtacatccac ccgaattcca ttttagaaat aaaaggtact cttcaccgaa atccataaaa
     3661 gaatcaccac ttaaggcatt caagattgtt caagttcaac aacaaacggc ataggttcag
     3721 acaaattatt tcctctaacc tttgaaaata ctgtggtgtt aaaataggca cagcttttac
     3781 catccccgga tattaaaaca tatatgtttt ccaccttaat ccagcattcc gttttgaagt
     3841 aatcactaat actttttacc attgccttct cgcactcttt tacatcaata ttgaactctc
     3901 tgtttttaaa ctcttgcagg tattccatgt ttggaagaaa aactcctaca atctcgccct
     3961 gggcggtgac cgatacctct ggcatggttg caccggcctt gtaaccgtca aactccatag
     4021 aatagctgta aactccaatg tcactaaaac tgtttgcatt gcggcgcggc atgttaactt
     4081 catccacctt gaaatctccc aaatcaacat acttgctcag aaactcattt gctatctctg
     4141 ccagatcctg tttatttttt atagacctcg cctctgtcac ttttaaatta tgaattctgt
     4201 gcagtttatc tgttcctgaa acgaaaaaga tcatcggctt gttgatgctt ccgttaaaat
     4261 acggttcggc attcaacact gaataaacat cgacatcata accaaagctt accccttttg
     4321 gcctggtata ttcgtactga agctcatatg taacaccatt gatttcatat tccattcgtt
     4381 ttcccgacaa cttatccaaa acaaccttat ccggatgcaa atccttatca tgcacacggc
     4441 aattgtagac tgttaaataa ctttcttcaa cttccacttc agcttcttca caggctgatg
     4501 cggcaaacaa taaaacaaga cacaagacaa aaacatgcac cgcttaatca ttacagcctc
     4561 ctgtaattct tttacgttat tgctattatc agtatagcaa tttttggttt ttgccgtgtg
     4621 tcctttatgt aaatattcct tattcaaact gtgaatagta catctcgtag tacatgccct
     4681 ttttcttcaa aaggtcctca tgtgttccca tttccacaat gttgccttca tccaccacaa
     4741 gtattacgtc agaattcctt atggtggaca acctgtgggc aataacaaag caggttttgt
     4801 ccttcatcag gtttctcatg gctttttgaa tcttaatctc ggtattcgta tccacattgg
     4861 atgtggcttc gtcaagaatc agtatttttg catctatcag catggctcgt gcaatagtaa
     4921 gaagctgttt ctgccctttg gatatgttgg cagcatcatc actgaggact gtatcatatc
     4981 cctttggcaa tttttcaata aaggagtgta tcattgcagc ttttgcagct tcctcgactt
     5041 cctctcttga tgcattttcc ttcccatacg caatgttttc aaatactgtt ccgctgaaaa
     5101 gccaggtatc ctgaagcacc atggcataag actttctcaa actcgccctg gttatgtctt
     5161 caacaggaac accatcaaca agtattattc catcatccac atcgtaaaat ctcataagaa
     5221 gattaatgag ggttgttttt cccgcaccag tcttccccac aattgcaacc aaacttcctt
     5281 tgggagcaat caaatttaaa ttctttataa ccaacttatc ctttgtatac ccaaagttta
     5341 catcctcaag ctttacatct ccttccacac catcaagaac aactgcatcc ttggagtctt
     5401 ccttctcagg ttcctcgtct agcaatcgaa agaccctttc agcagcagcc agtgctgact
     5461 gcaattctgt tgcaatattg gccatctcgt taatcggtcc cgagaacttc ctggagtaca
     5521 atacaaagga tgcgatatct ccaagactta tcgttctgta taggtacatg atggcaccaa
     5581 acacactgat aagcgagagc gacaggttgt tgacaaagtt tatggaaggg cctgtcatgc
     5641 tgccgtaata ttctgactta tagtaagcgg tggcagtctt ttcgttatag tccacaaact
     5701 tgtcaattgt agaatcttca ctattgtatg ccctcaaggt tttttgtccg gttaccattt
     5761 cctcgacaaa gccattcatt tcgcccagtc ttgcagacct tgccttgaaa agtggactcg
     5821 ttctttttgt tatgaaccta gttaaaaata tggatgtggg tactgtaaat gcaaacacaa
     5881 gcaccagata tggagatatc accagcatca tgacaagaga tcctatgacg gttataaagc
     5941 tggcggatat ctgtacaagg tccgatgaca gcgaagtatt tatagtatca atgtcatacg
     6001 acatcctgct gataatgtct cccgtaggat tcacatcgaa aaatcctacc ggaagcgttg
     6061 ttatcttctc aaacacatct ttcctcatgt tgtaaacaac ctttcggctt atgtaaacca
     6121 tgagcaagga taacagatac gtaagaatcg aggacaaaag gtagacaaca agcatcaaaa
     6181 cagctatttg aaaaactttt tgaaaattga cactacccgt tcccggctca atatagctga
     6241 tggctttacc agacatcatg ggaccaatga ggttgaaggc attgccgatt atggtaagca
     6301 ccaatgccag aacgagatgc catttgaaat ccttcatgta ttgcaaaagc ctaataaccg
     6361 tatgcttttt tttcttcata ccagcatctc tcccatctgc gtgcttgcaa tctcctgata
     6421 cagctcgttg gatttaatca gttcatcatg cgtgccatac cccagtatcc ttccttcatc
     6481 caaaacaata attcgttctg catgttttat ggaacttatc ctctgagcaa taataattgt
     6541 ggtagtacct ttgtagtgtt tcttgagttc ccttcgcagc gaagcatccg tcttgtagtc
     6601 aagagcactg gatgcatcgt ccagtatcaa tatttcaggc cttcccgcta gtgcccttgc
     6661 aattaaaagt ctttgcttct gtccacctga gaaattggct cccttgatgg acagcatgaa
     6721 atcaagacct acacctttta caaaatcaga cgcctgggca tgctctatcg acctgctgat
     6781 atcctcaata ctgatttccc ttccaagctt aatattctca gctactgtat ctctgaaaag
     6841 agcatcgttt tggaatacaa cgccaaacct tcttctaaga tccttaaggt catagcttct
     6901 tacatccttg ccatcaattc tgatggagcc tttgtcgggg tcgtagaatc tcatcaacag
     6961 ttttaacaaa gtgcttttac cagaacctgt cgccccgata attcccaaag tctcaccctt
     7021 tttaatccca aaggaaatat ccgaaacatc atccttcact ttgttgtatg aaaatgacac
     7081 gttttcaaag gtaatgaaat cctcatcgtc tttttccagg atgttttcaa aagatgttgt
     7141 atcagatggc ttaacttcca gaacttcctg aattctattg gcagaagcaa tggaccttga
     7201 gaataccgta aatattcttg taatggaaag catggcattt agaataatag tgaagtatgt
     7261 aaggaaagca acaatctttc caacctctga aacacctgca tttactctaa aggctcccaa
     7321 caatattact cccacaagac cgccctgaag gagaaagttc atggcaggct ttattgtatt
     7381 gagaaccatg tttgccttgt tctcatcatt cattaccttt ttattcacct tgtcaaacct
     7441 atctttttca taatccgact tggataatgc tttaataacg cgtacaccgg taatgttttc
     7501 tctaagaacc cttatcatat cctccgtgga ttcctgcaca gttctaaaca gaggaatgcc
     7561 cttggaagac actgtgtatg taataaaaac tagaaataca agtacaacca ccaatacaag
     7621 ggtaagaacc ggatcaagag ttaacgtgac caaaaggccc cctattaata aaattggagc
     7681 ccttatgcca attctctgca tcattcccac cattcgatgc acgttgtatg tatccgatgt
     7741 cattct
//
LOCUS       DAONVY010000032         7684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7225_length_7684_cov_3.343557, whole genome shotgun sequence.
ACCESSION   DAONVY010000032 DAONVY010000000
VERSION     DAONVY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7225_length_7684_cov_3.343557"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..2868
                     /locus_tag="PLI11_01610"
     CDS             193..2868
                     /locus_tag="PLI11_01610"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HPZ51592.1"
                     /translation="MNVSEKQQWHQISVVETEKLLATSMELGLDDNEVQSRLEKYGPN
                     EMAKGKKKSILSMFAAQFKDFMIVVLIVAAIISFVVTRDVAESIIILLIVILNAVIGV
                     VQEFNAEKSLAALQKLSSPSTKVLRNGQSMVLPSTELVPGDIVILETGDLIPADLRLI
                     ESFNLKVQEAALTGESVPVDKDATVVFNEAQDGKETPLGDRINMAYASCLVTYGRGTG
                     IVTGTGMETEVGKIASMLMNVESRETPLQRKLNQLGRYLGIAALIICVVMFGIGVLND
                     KGLMEMFMVSVSLAVAAIPEGLPAISTIVLAMGVKKMVERNAIMRNLPSVETLGSTSI
                     ICSDKTGTLTQNKMTVVKLWANGKTMDAENTEELLSDDTFKQVILTSILCNDSKISNN
                     GEGLNLIGDPTETALIDLGLKVGLDKRNLDNEYQRIYEVPFDSDRKLMSVVCKSDNGG
                     LRVYTKGAVDVLLAKCNLSEEEKERIYQANEELASMALRVLALACKDIDVAPESDEGI
                     EKNLVFIGLLGMIDPPRPEAKEAVKKCKTAGIKPIMITGDHKTTAVAIAKDLEILNEG
                     EMAIEGSELNTISDEELQEKVENISVYSRVAPEHKVRIVNAWKEKGHIVAMTGDGVND
                     APALKSADIGCAMGITGTEVAKEAADMVLTDDNFATIVAAVEEGRRIFDNILRAVYFL
                     LSCNIGEIVALFTATIFLKAFGGEQPLIAIQILWVNLVTDSLPALALGIEPAEKGIMK
                     RKPLPFNSPIFTKASFARLLYQGVSVGMLTLVAYQIGYRTTGLTQVGQTMSFAVLAFS
                     QLFHVFNLKSIKDSIFKTNLVNNPKLLGAVAISAISMLVVLLVEPIQKIFKVTSLNLS
                     QWLIVLGLSASIIFIVEIVKFFTNKLEKLKGKAYD"
     gene            2861..3646
                     /locus_tag="PLI11_01615"
     CDS             2861..3646
                     /locus_tag="PLI11_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPZ51593.1"
                     /translation="MIDTHIHSRFSTDSETNPLSFILKAIDLGLDAITFTDHYDLEYQ
                     DGIIMHFDIDEYFNELSELKEKYKDKIKILIGIEVGIQSNISDKIKNAIKVYPFDYII
                     GSTHLIRGIDPYYRQLYNENTKKQDALDEYFDIIYENITSFNIFDTMGHIDYLKRYTP
                     FEDNGIYYHENKEKIDRILNYLVSKDKAFEINTSGIDVHPFDFDILKRFKELGGRYVT
                     LGSDAHNQEYLCRHFNSTIEKIKLCGFDKITYYENRNPVQINI"
     gene            complement(3715..7017)
                     /locus_tag="PLI11_01620"
     CDS             complement(3715..7017)
                     /locus_tag="PLI11_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ51594.1"
                     /translation="MTKKVLTILIIICIFSLQLSVGVFAERDHMADTWVGNDALGRRL
                     PTNEQVGDRKEEKYVGIFYFLFMNAEQGRILDHSKVFAEDGVEAVWDVLVQDGMHMWG
                     GPYFGYYKNTDEWIYRKHAQMLVDAGIDFVFLDTTNDGGLFPHAWQILLDTWAEIREE
                     GGMTPYVVFHCGVNAPTMKSHMPTIFKAYQNPKYNDLWFRWEGKPLILGDYTMLEQKY
                     FDFFTLRESWAFNGWTGDGKGKWPWIAEVPQEPGRSFDGVIEQIVVCAGYHSNSSKGR
                     SFSKEKQPLTGRLDFGFSLDDTDKGIAFDEQWKQVFKVDPKVVMITGWNEWTMGRWEN
                     AGLGQKISGTYILTENDLMFMNNYVDCFNVEYSRDIEPMNGFHGDNYYYQMASYIRKF
                     KGARPIPMGTGSKVINDLKDLSVWDSIGPEFYDTKNDIAHRDHPSVCGLFHYENQTGR
                     NDIEYIKVSKEAGKTYFMIKCVDDIIVDMEGENWMNLFIDSDRYYGTGWNGYDYVINR
                     SRDGKTMTVERFVNNDWKFEKAGTAEYALNGKTLVIEVDDSLIGLSDRDDFDFKCADN
                     STITGEAMEFMDLGDAAPNGRFNFRYMKEGGRIKGDGTGDATSSSSLNNTTIGVIILA
                     SCVLVSVFGVIVAIRIMQKRNIKPKNLVVALVFVIYMSSTMFYNQSHALFSSKDLVLV
                     VKKDISEYGNHKLQKEVINEYVEAFNKINPKIKVKVKYVNDFNGNLNDGDVLLINATT
                     ASDFMAAKKYDKTDNSSKLLDLTKYLDNTENMLEPALRLGQLDGRQYWIPVNYDRTVV
                     YIDKSVFDMRGKGIPSKDWTYDEFKQLVHDLTFSEKGNRYTGVYLNHHQMNVWRLFAE
                     GFGGSMYNSDAKMIELSKGKAVEGLSEMFLFLEDGYAKGLGFNTKGSDRSNSAMSIAF
                     AFQPQRNGRYLLSDEEFRSNPATKSLVDKDNIVILPLPEFPEGRIGTTNADFCIGFAV
                     NKSAKNKDNAARLAKFALTEQGQKILNKYYGGIPANKSTWNDDYWKKGLQGDFVENIL
                     YGIENDKRNDFFELYVETEDPYYYALRTRVFYTCYMNRDYERIQKRPSGNFKQYLERF
                     EKDINKMIGQIT"
     gene            complement(7105..>7684)
                     /locus_tag="PLI11_01625"
     CDS             complement(7105..>7684)
                     /locus_tag="PLI11_01625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPZ51595.1"
                     /translation="WVQKHSLGPTRRKVVEVIKRLEVGDSFNLLDVGCGTGQFIDEIK
                     YEFENVKTFGMDYSEGMLQEARNKNNDTSYFRGNICASIPKEFIEPESIDIVTCMHSF
                     PYYPDKAGALMNIKAILKTGGYAIFCSASVNCLYEKFVMWFVGKTASDAEYLSKNDMR
                     ELLAKEFVLIDEFNIKRRFYMPTICGFLVRKI"
BASE COUNT         2371 a         1552 c         1427 g         2334 t
ORIGIN      
        1 caaaagcgga aggtagacga cttataaaac aaggcggcgt ttatgtaaac gatgaaaagg
       61 tgccaaattt tgattatgca atacctaccg aacagcttga aggcgatgga gtattgatta
      121 gaaagggcaa aaagacatat catcgcgttt acgtaaacta aaaaacttaa tataaaggag
      181 aatctatcga taatgaatgt ttctgaaaaa cagcaatggc atcagatttc cgtggttgaa
      241 acagaaaaac ttcttgccac ttccatggaa ctcggtcttg atgacaatga agtacaaagc
      301 agacttgaga aatatggacc aaacgaaatg gcaaagggga agaaaaagag tattctttcc
      361 atgttcgcag ctcaattcaa ggacttcatg atagtggtgc ttatagttgc agcaatcatt
      421 tcgtttgttg taacaagaga tgtggcggaa tcgataataa tactcctgat tgtaatattg
      481 aatgcggtta taggagtagt acaggagttc aatgcggaaa aatcacttgc tgcattgcaa
      541 aagctttcat cgccatcaac gaaagttttg agaaatggac agagcatggt tctgccgtca
      601 acggaacttg tccctggtga tatcgtaata ctcgaaaccg gtgatttgat tcctgcggat
      661 ttgagactaa ttgaatcctt caaccttaaa gtgcaggaag cggcattgac tggtgaaagt
      721 gttcctgttg acaaggatgc aactgttgtt ttcaacgaag cacaagatgg caaggaaaca
      781 ccgcttggtg acagaataaa catggcttat gccagttgtc ttgttactta tggtagaggc
      841 actggcattg ttaccggcac tggaatggag acagaggttg gcaagattgc aagcatgctg
      901 atgaatgtcg aaagcaggga aacaccactg cagagaaaac ttaatcagct tggaagatac
      961 ctgggcatag cagcattgat tatctgtgta gttatgtttg gtattggagt attgaatgac
     1021 aagggcttaa tggaaatgtt catggtttca gtaagccttg ctgtagcagc catacctgaa
     1081 ggacttccag ccatctccac aatagtgctt gccatgggcg ttaaaaagat ggttgagaga
     1141 aacgcaatca tgcgaaatct tccctctgtt gagactttgg gttcaaccag tataatatgc
     1201 tcagacaaga ccggaaccct tactcagaac aaaatgacag tagtcaagct ctgggccaac
     1261 ggaaaaacca tggatgcgga gaacacggaa gaactgcttt ctgatgatac tttcaaacaa
     1321 gtaattctca catctattct ttgcaacgac tccaagattt caaacaatgg cgaagggctc
     1381 aatcttatag gggatcccac tgaaacagca ttgattgatt tgggtctcaa ggtaggactt
     1441 gacaaacgta accttgacaa cgagtatcaa cgcatttacg aagtgccttt tgattcagac
     1501 agaaaactta tgtccgttgt atgtaaatct gataatggag gccttagggt atatacaaaa
     1561 ggcgcagttg acgttttgct tgcaaaatgc aatctttccg aagaagaaaa ggaaagaatc
     1621 tatcaggcaa acgaggaact tgcttccatg gctttaagag tacttgctct tgcttgcaaa
     1681 gatattgatg ttgcacctga aagtgatgaa ggtattgaga agaaccttgt attcataggc
     1741 ctgttaggta tgatagatcc tccaagacca gaagcaaagg aagctgtaaa gaaatgcaaa
     1801 acagcaggca taaagcctat tatgataacc ggagatcaca agactactgc agtcgccatt
     1861 gcaaaggatc ttgaaatttt gaatgagggc gaaatggcca ttgaaggctc cgaactgaat
     1921 accataagtg atgaagagct tcaagaaaag gttgagaata taagtgtata ttcaagagta
     1981 gctcctgaac acaaggttag aattgtcaat gcgtggaaag aaaagggcca tattgtcgcc
     2041 atgacgggag acggggtaaa tgatgctcct gcattgaaat ccgccgacat tggttgtgca
     2101 atgggtatta ccggtactga agttgccaag gaagccgcag acatggttct taccgatgac
     2161 aattttgcaa caatagttgc agcagttgaa gaaggcagac gcatattcga caacatatta
     2221 agagcagtat acttcttgtt gtcgtgtaat ataggagaga ttgttgcact gtttaccgct
     2281 acaatcttct taaaagcatt tggcggagaa cagccactta ttgcaattca gatattgtgg
     2341 gtaaaccttg taacggacag cctgcctgct ttggctttgg gaattgagcc ggcagagaag
     2401 gggattatga agagaaaacc tcttcctttt aacagtccaa tctttactaa agcttctttc
     2461 gccagattgc tctatcaggg tgtttctgta ggaatgctta cgctagtggc ttaccagatt
     2521 ggatacagaa caactggatt aacacaagta ggacagacaa tgtcatttgc agtattggca
     2581 ttctcacagc tgttccatgt attcaacctt aagagtataa aggattctat tttcaaaacc
     2641 aatctggtta acaatccaaa actcttggga gcagttgcaa tttcagccat aagcatgtta
     2701 gtagtactgc ttgtagaacc tatacagaaa atattcaagg taacatcctt gaacctgagc
     2761 cagtggttga ttgtgttagg actttcagca tcaattatat ttattgtaga gatagttaaa
     2821 tttttcacaa ataaactaga aaaattgaaa ggtaaagcat atgattgata cacacataca
     2881 ctcgagattt tctacggatt cggaaacgaa tccgctctct tttattttaa aagcaattga
     2941 tttggggcta gatgccatca catttacaga ccattatgat ctagaatatc aggatggcat
     3001 cataatgcat tttgatattg atgagtattt caacgagctg tcagagttaa aagagaaata
     3061 caaggacaaa ataaaaatcc ttattggcat tgaagttggc atacagtcca acatatccga
     3121 caagattaag aatgcgatta aggtttatcc ctttgactat attatcggtt ctacacacct
     3181 cataagaggt atcgatccat attacagaca gctgtataac gaaaacacaa aaaagcagga
     3241 tgccctggat gaatactttg acatcatata tgaaaacatt acttcattta atatatttga
     3301 taccatgggt catatagact atttaaaaag atacacacca tttgaagata acggaatata
     3361 ttatcatgaa aacaaagaaa agatagacag aatactaaac tatttagtta gcaaggataa
     3421 ggcttttgag attaatacat caggtataga tgtacatcca tttgattttg atattctaaa
     3481 aagatttaaa gaactgggtg gaaggtatgt gacacttgga tccgatgctc acaaccagga
     3541 gtatttgtgt cgccatttta attctacaat tgaaaagatt aaactttgtg gttttgataa
     3601 aattacatat tacgaaaaca ggaatcctgt acagattaat atctgatagg ttagagatag
     3661 cacattgaac aaaataataa ttttgttcaa tgtgtgctct aaaaaggcca aattttaagt
     3721 aatttggcca atcatcttgt ttatatcctt ttcaaacctt tcaaggtatt gtttgaagtt
     3781 tcctgaaggt ctcttttgaa ttctctcgta atctctattc atataacatg tataaaaaac
     3841 tcttgtgcgc aatgcgtagt aatatggatc ttcagtttct acatacaact caaagaaatc
     3901 atttctcttg tcattctcaa ttccgtatag aatattttca acaaaatctc cttgcaaacc
     3961 tttcttccag taatcatcat tccatgtact cttgtttgca ggaattccac catagtattt
     4021 attcagtatt ttttgtcctt gctctgtcaa tgcaaatttt gcaagcctcg ctgcattatc
     4081 tttgttcttt gcagatttgt ttacagcaaa gccaatacag aaatccgcat tggtcgtacc
     4141 aattcttcct tctggaaatt caggcaaagg caaaattact atattatcct tgtcaactag
     4201 tgattttgtt gcaggatttg acctgaactc ttcatctgat agtaaatatc ttccatttct
     4261 ttgtggttga aatgcaaatg caatggacat ggcactgttt gacctgtcgg agcctttggt
     4321 attaaaccca agacctttgg cgtatccatc ctcaagaaac aagaacattt cagaaagtcc
     4381 ttctactgct ttgccttttg aaagctcaat cattttagca tcgctgttat acattgaacc
     4441 tccaaagcct tctgcaaaca gtctccatac attcatctga tggtgattga ggtaaacacc
     4501 tgtgtatcta ttacctttct cagagaaagt aaggtcatgt accagctgtt tgaattcgtc
     4561 ataagtccag tctttggatg ggattccttt acccctcata tcaaacacac tcttgtcaat
     4621 atatacaact gtacggtcat agtttactgg tatccaatac tgtctgccat ccaattggcc
     4681 aagtcttaaa gctggttcaa gcatattctc tgtgttgtcc agatactttg tcaaatcaag
     4741 aagcttgctt gaattatcag ttttgtcgta tttctttgcg gccatgaagt ccgatgcagt
     4801 ggtggcgttt ataagtagga catctccatc attaaggttg ccgttaaagt cgttcacata
     4861 cttgactttt acctttatct tcggatttat cttgttaaat gcttctacat actcatttat
     4921 gacttctttc tgcaatttat gatttccata ctcggatata tcctttttta ccacaagaac
     4981 caaatccttg cttgagaaca atgcatgcga ttggttgtaa aacattgtgg aggacatgta
     5041 tatgacaaat actaatgcaa ctaccaggtt cttaggcttg atgtttcttt tttgcataat
     5101 tctaatagcc acaataacac caaaaacaga aacaagcaca caagaagcca atataataac
     5161 tccaattgtt gtattgttta agctggagga agaagttgca tctcccgtac cgtcaccctt
     5221 tattctgccg ccttctttca tgtatctgaa gttgaacctg ccattaggag cggcatctcc
     5281 taaatccatg aactccatgg cttcaccagt aattgttgaa ttgtcggcac atttgaagtc
     5341 aaagtcatcc ctgtcagaaa gtccaatcaa agaatcatcg acctcaataa caagggtctt
     5401 tccattcaaa gcatattcag cagtgccagc tttttcaaac ttccagtcat tattgacaaa
     5461 tctttcaaca gtcatggttt tgccatccct ggaccgattt attacatagt cataaccatt
     5521 ccatccggtg ccataatatc tgtcgctgtc aataaacagg ttcatccagt tttcgccttc
     5581 catgtcaact atgatatcgt cgacacattt tatcatgaaa tatgtttttc cagcctcctt
     5641 ggatacttta atatattcaa tatcgtttct tcctgtctgg ttctcataat ggaacaaacc
     5701 acaaacggat ggatgatctc tgtgtgctat gtcatttttt gtgtcataaa actcaggacc
     5761 aatggaatcc cacacagata aatcctttag gtcattaata actttgcttc ctgtccccat
     5821 gggtattggt cttgctcctt tgaactttct tatgtatgaa gccatttgat agtaatagtt
     5881 atcgccatga aaaccattca taggctctat gtctcttgaa tactccacat tgaaacagtc
     5941 cacatagttg ttcatgaaca ttaggtcatt ctcagttaga atgtatgtac cggagatttt
     6001 ctggccaagg cccgcatttt cccatctgcc catggtccat tcattccagc cagtaatcat
     6061 gacaaccttg ggatccacct tgaatacctg tttccactgt tcatcaaaag caattccttt
     6121 atccgtatca tctaaagaga atccaaaatc caatctgcca gttaaaggct gtttttcttt
     6181 ggaaaaactt ctgccttttg atgaatttga atgatatccc gcacaaacaa caatctgctc
     6241 aatcacacca tcaaaacttc taccaggctc ttgaggaact tccgctatcc atggccattt
     6301 tcctttacca tcaccagtcc agccattgaa tgcccaggat tctctgagag taaagaaatc
     6361 aaagtacttt tgttctagca ttgtatagtc tcctagaatc aagggttttc cttcccacct
     6421 gaaccacaag tcattgtatt tgggattctg gtatgctttg aatatggtag gcatgtggga
     6481 tttcatggtt ggcgcattta cgccacaatg gaataccaca taaggagtca ttccgccttc
     6541 ttcacggatt tccgcccagg tatccaatag gatttgccat gcatgaggaa atagtccgcc
     6601 gtcgtttgta gtgtctagaa atacaaaatc tatgcctgca tcaacaagca tctgcgcatg
     6661 ttttctgtaa atccattcat ctgtattctt gtagtagcca aaataagggc cgccccacat
     6721 gtgcataccg tcttgtacca ggacatccca tacagcttca acgccatctt ctgcaaagac
     6781 cttcgagtgg tccagaattc tgccttgttc agcattcatg aacaggaaat agaagattcc
     6841 cacatatttc tcttcctttc tgtcaccaac ctgctcattt gttggaagcc ttctgccgag
     6901 agcatcattt ccaacccatg tatccgccat atggtctctt tctgcaaata cacctacaga
     6961 caactgtaaa ctaaatatgc agataataat aaggatagtt aaaacctttt tggtcataga
     7021 atcacatccc gtctacacag tcattaacac ttgtttatat tatacaacaa aaccttgcta
     7081 cagtataacg ctaaaccaac attttcatat ctttctgact aaaaaaccac aaattgtagg
     7141 catatagaat cttctcttaa tattaaactc atcaattagg acaaattctt ttgccaaaag
     7201 ctctctcata tcattttttg ataaatattc ggcatcggag gcggttttcc caacaaacca
     7261 cataacaaac ttttcataca aacagtttac agaggctgaa caaaagattg catatccgcc
     7321 agttttcaat atggctttta tattcatcaa tgcacccgcc ttgtctggat aatatggaaa
     7381 tgaatgcatg catgttacaa tgtcaatgct ttcaggttct ataaactctt ttggtattga
     7441 tgcacaaata ttccctctaa aataacttgt atcattgttt ttgttccttg cttcctgaag
     7501 cattccttct gaataatcca tgccaaaggt tttgacattc tcaaactcat actttatttc
     7561 atcaataaac tgtcctgttc cgcagccaac atcaagcaga ttaaaagaat ccccaacctc
     7621 aagtcttttt atcacttcaa caacctttct tctagttggt cccaaagagt gtttttgtac
     7681 ccac
//
LOCUS       DAONVY010000033         7487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7472_length_7487_cov_3.598493, whole genome shotgun sequence.
ACCESSION   DAONVY010000033 DAONVY010000000
VERSION     DAONVY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7472_length_7487_cov_3.598493"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..834
                     /locus_tag="PLI11_01630"
     CDS             76..834
                     /locus_tag="PLI11_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HPZ51596.1"
                     /translation="MSRTRKRKSLIDSIAELIWPHVRGWLGETSVNLRLKLLLPEEYQ
                     VVNNVILPTETGTTQIDHIVVSPYGIFVIETKNYKGWIYGNDQSREWTQTLYKNKYKF
                     MNPIHQNYAHVKAIESRLSNYPNIPIIPIIAFSKDCRLKVKTRQNVVYFNQVKSVIRK
                     FKSKVMSIQEMMEILALLQHGNEQFSISSKEHIENVNYKKTLIKYASAGDKCPRCNTG
                     FLSVKQGKHGKFLGCGNYPKCRFTKQLANRVDGQ"
     gene            891..1682
                     /locus_tag="PLI11_01635"
     CDS             891..1682
                     /locus_tag="PLI11_01635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPZ51597.1"
                     /translation="MNIKDYYASSDEKPLERMPADGGFTGIFRTICCIGDSMSSGEFE
                     SIDDNSNKEYHDMFEYSWGQYLGRLVGAKVLNFSRGGMTAIEYCKTFADANNFWDTDK
                     LCQCYIIALGINDLVGLKMPVGSIEDINYEDYTKNNEETFAGWYAQIIQRMQALQPKA
                     RFFLVTMIREDSEEHNKIVQAHAQLLHDIAERFDNTYVIDFAQYGPVIDAEFRRNFYL
                     GGHLNPAGYLLVSRMVASYIDYIIRNNPEGFYEVPFIGTPYSFSK"
     gene            1864..3042
                     /locus_tag="PLI11_01640"
     CDS             1864..3042
                     /locus_tag="PLI11_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51598.1"
                     /translation="MKKIIFGCAIILLFVFGIGSYMVYSKPIDYISIDINPSVELGVN
                     AFNKVVSARAVNKDGKKLLGDAKVVNLDVSDAVATLLKVAADKEYIAEDGSSVVAITA
                     ESKNQKKAERLQRICGEAVEYAEKKMNTRIAVYKNACGPSLRQEANELGVTPGKLKLI
                     KTLQVLEPSATVEEYKDAKVADIICRIKELIAENEEVNIDEEILERIEETCPDDKRSE
                     LEELRENTKEELCDIKEQIGEVNREAVEKVNEIRTQITEILASLMDMTEEEKEDTLRM
                     LEELKKQAEAIKKEAKKAIDELKELADSKKGEIDKQLEEIKELIEEHKKDLEGKREDD
                     DKKCEEKQKKAQEKYEEALKRAEEKREKAMKKYLEELKKAQEKLEKARKKQQNNCKGK
                     "
     gene            complement(3099..4919)
                     /locus_tag="PLI11_01645"
     CDS             complement(3099..4919)
                     /locus_tag="PLI11_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51599.1"
                     /translation="MSRKKLLILLSFLCLSMIVVLTACTGGSNEDEKTPEPTEDPRIF
                     RDELPRVEPREDQHITFKTIDDGFDVFAPLAGHWGYRYGPSILFYPDGTMDAWFATPG
                     TSGEWDWFTYKHSDDRGATWSNEIVVLQPTPDSMDHYSVCDPGLIYFDGWYYLGYTST
                     IVSTNGGINNNIFVARSKNPDGPYEKWNGSGWGGDPAPIIYFDEADSAWGAGEISFVE
                     LDGTLYCYYTWRCPDGDFTKVSIADSTSENWPETLEYKGIAYKYGPGQDSCDVFYVED
                     IGKFIALSTIHRFTTDSGIAIMESNDGINFTHIDTIRTGISKFCHNMGVSKRPNGHVQ
                     LKDELIIGYAYSDGGQENWGKWATRFQKIKLEVYDGEAKQTDRDGKNVLRGDYFWEEL
                     PKEELWPIAIGTLPQKIEVHLKDGTYKVPAKWYDTYLRSHDITDEDKVKFTDYDKEII
                     DFDGLTMKMKKVGKTNVTMHYEGRQITFKVYVRPDDFQIGMENPEIVSFTPVEEHITV
                     YKKDYGVRHKKQIRGFVVFEDETWGEAYNDSTKAHPDYPPKVPREKYEMKFEVADTSV
                     ITVNNRGIITAKNVGTTTVKVTITGGHSFTVTVTVEEELP"
     gene            5035..5508
                     /locus_tag="PLI11_01650"
     CDS             5035..5508
                     /locus_tag="PLI11_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51600.1"
                     /translation="MKDIKENSGKENWCLRILVTVKSTSRKRNYITGKEIIVSDNNRT
                     MKDLLCDLACQLHDAYISKSIDADIVAALTEEELSDGQESGKVGFGRRYGEGVVSREK
                     AIETMLLVFEDGLFRVFLNNEEVVDINEAINIKDGDNLILVRFVMLSGSCFSYVF"
     gene            5518..6030
                     /locus_tag="PLI11_01655"
     CDS             5518..6030
                     /locus_tag="PLI11_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51601.1"
                     /translation="MFLTSYREKEAQFYKEKERRLPKSNDYYSQLHNAILYQMYYHYN
                     HETWTEDKVFDLIGEKHESLYGLFDGEGFAILEFLFENKASMIRDIWKRTFDFGYQSG
                     FERRPFRTKHSIGLHYRRMISFLSGFAYLSVLNFNLEEYLKDPNKSENTYFTYNGRKA
                     ISYTMAHEID"
     gene            6207..>7487
                     /locus_tag="PLI11_01660"
     CDS             6207..>7487
                     /locus_tag="PLI11_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51602.1"
                     /translation="MIDEGDADNFIYLLKVILDNKMERFSSVVRAFDVWTGLPVSAEK
                     SKTARYYLEVIYDCVANRHEEKYLASPNPVENHIALMCIGFYELQDVYKVLDDFLGNN
                     DPAKRIAGWYLLSQTADPQAQLEYFIKYNDERTPDVLAYMADSLVAKLKYGHELMFEK
                     LLKMINETPIKTKTVSYPGMQELTRKYSQEFMYNGLVDLAKSAGAGLKDSMFDTLCDY
                     YERIPVDTRIVFVENLSNNMTTKKHRQTILNAACDKSKFVRIIAASKLKEMKLSEEEY
                     MELESMLRHKTESLRKNIMVLLLEQSPEDCLESVRRLHNSQDANCKLAARQMVMDMQS
                     DSRFAKVLSKAKEIVSMEEVVAESDENVYGLVDYGKLTKLAVPKKKPSYKFKDALPDL
                     MELEKKVHKLTEVVKRHVNEVYYSENEEIVYGSER"
BASE COUNT         2380 a         1335 c         1669 g         2103 t
ORIGIN      
        1 attgacaaca ttgtggtgat ggtagaggag caagtatccc acagaaggat aatgccatga
       61 aaggagtttt taataatgtc tcgtacaagg aaacgtaaat ctttaattga ttctatcgct
      121 gaattaatct ggccccatgt tagaggttgg ctgggagaaa catctgtaaa cttgcggttg
      181 aaactcctct tgccggaaga atatcaggtt gtcaataatg ttattctacc aacggaaact
      241 ggaacaacgc aaatagacca tatagtggta tctccatatg ggatatttgt tattgaaacc
      301 aagaactata aaggttggat ttatgggaat gaccagtcca gggaatggac ccagactctt
      361 tacaaaaaca agtacaaatt tatgaatccc attcaccaga attatgctca tgtaaaagcc
      421 attgaatcaa gactttcaaa ttatcctaat atccccataa tcccgataat tgctttctca
      481 aaggattgca gattgaaagt aaagactaga caaaatgttg tgtatttcaa ccaggttaag
      541 tcagtgatac gaaagttcaa aagcaaagtt atgagtattc aggagatgat ggagattctg
      601 gctttgttgc agcatgggaa tgagcaattt tcaatctctt ccaaggagca tatagaaaat
      661 gtaaactaca agaaaacact cattaaatat gcttctgcgg gtgacaagtg tcctaggtgc
      721 aacacaggat ttctgtcagt taaacaggga aaacacggta agtttttggg gtgtggcaat
      781 tacccaaaat gcagattcac aaaacaactt gcgaatagag ttgacggaca ataattgaag
      841 tagtagaatc tgagcgtatc agattgcttt gaaaggatgg aattagcaaa atgaatatca
      901 aagattacta tgcaagtagt gacgaaaaac cacttgaaag aatgcctgcg gatggaggtt
      961 ttacaggtat tttccgtacc atttgttgta ttggagacag catgtcttct ggagaatttg
     1021 aatcaattga tgataatagc aacaaggaat atcacgatat gttcgaatac tcatggggac
     1081 agtatttggg acgccttgtt ggtgcaaagg ttcttaattt ctctcgtggt ggcatgactg
     1141 ccattgagta ctgcaaaacc tttgcagatg caaacaattt ctgggatacg gacaagctct
     1201 gccaatgcta tattattgct ctaggcatta atgatcttgt agggcttaag atgcctgtag
     1261 gttccataga agatatcaac tatgaagact acacaaaaaa caatgaagaa acttttgccg
     1321 gatggtatgc gcaaataatt caaagaatgc aggctctgca gcccaaggct cgtttctttc
     1381 ttgtaacaat gatacgagag gacagtgaag agcacaacaa aattgttcag gcgcatgcac
     1441 aacttcttca tgacattgca gagcgttttg ataacaccta tgtgattgat tttgctcaat
     1501 acggtcctgt gatagatgct gaattcaggc gtaatttcta tttgggaggc catcttaatc
     1561 ctgctggtta tctgcttgtt tccagaatgg ttgcttccta cattgactac attatccgca
     1621 acaatccgga aggcttctat gaggttcctt tcataggcac cccttatagc tttagtaaat
     1681 agctttttgt tgtacatttt acttagactg gagcaagaat cttttaactc aggaagtcaa
     1741 gccaaatacg aaaaaagaaa cagaatgcaa ttctgcgctc tgtttctttt ttgctttacc
     1801 ttgaaatgtt agaagtttgt tacgaaagta tttactgaaa acaaaaaaca taggggtgaa
     1861 agaatgaaaa agataatatt tggttgtgca attatactgc tcttcgtatt tggtattggc
     1921 agttatatgg tttacagcaa acctattgac tacataagta ttgatatcaa tccaagcgtt
     1981 gagctgggcg tcaatgcttt taacaaggtt gtaagtgcaa gagcggtgaa caaggatggg
     2041 aagaaactgc tcggagatgc aaaggttgtc aatcttgatg tatcagatgc tgttgcaact
     2101 ttgctaaaag ttgcggctga taaggagtat attgcagaag atggttcttc agtcgttgca
     2161 ataactgctg aatcaaaaaa tcaaaaaaag gcagaaagac tgcagcgtat atgcggtgaa
     2221 gcggtagagt atgctgagaa gaagatgaac actagaattg ccgtatataa gaacgcatgt
     2281 ggtccatcgt taagacaaga ggcaaatgaa ctgggtgtta caccaggaaa actgaagctc
     2341 ataaagacac ttcaggttct tgaaccaagt gcaactgtag aggagtacaa ggacgcgaaa
     2401 gtggctgata tcatttgcag gataaaggaa ctgattgccg agaatgaaga agtgaacata
     2461 gacgaggaaa tcctggagag gatagaggaa acatgccctg acgataaaag atctgaactt
     2521 gaggaattga gagaaaatac caaggaagag ctttgcgata tcaaggagca gataggtgaa
     2581 gtaaacaggg aagctgttga aaaggtgaat gagattcgaa cacagatcac tgagatattg
     2641 gcaagtctga tggacatgac agaagaggaa aaggaagata cgcttcgtat gctggaagaa
     2701 cttaaaaagc aggcggaggc tataaaaaaa gaggcaaaga aagctattga cgaacttaaa
     2761 gaactggcag attccaagaa gggcgagatt gacaagcagc ttgaagaaat caaagagctg
     2821 attgaggaac ataagaagga cctggaaggt aagagggaag atgacgataa gaaatgcgaa
     2881 gaaaaacaga aaaaagctca ggagaaatat gaggaagcct taaaacgggc tgaagaaaaa
     2941 agagaaaagg ctatgaaaaa atatctagag gaattaaaga aagcacaaga gaaactggaa
     3001 aaagccagaa agaagcaaca gaataattgc aagggaaagt agaaaggcat caaaaaagct
     3061 ccgtaagaaa tttacggagc ttttgatatt tgcatgattt atggcagttc ctcttccacc
     3121 gttacggtga ctgtgaagct gtgaccgcct gtaattgtaa ccttgacggt tgtagtacct
     3181 acattcttgg cggtgatgat acctctatta ttaactgtga taacagacgt atcggcaacc
     3241 tcaaacttca tctcatattt ctctctgggc acctttggtg ggtaatctgg atgagctttt
     3301 gttgagtcgt tatacgcctc gccccaagtc tcgtcttcaa atacaacaaa gccacgaatc
     3361 tgttttttgt gtcttacacc gtaatccttc ttgtagacgg taatgtgttc ctctactggt
     3421 gtgaaggata caatttcagg attctccatt ccgatttgga aatcatcggg tcgaacatag
     3481 actttgaagg tgatttgacg tccttcgtaa tgcattgtaa cgttagtctt tccgaccttc
     3541 ttcatcttca ttgtcaagcc gtcaaaatcg attatttcct tgtcataatc ggtaaattta
     3601 accttgtctt catcagttat atcatggctt ctaaggtatg tgtcatacca ttttgctggt
     3661 actttgtaag ttccatcttt gagatgaact tcaatctttt gtggaagtgt tccgatagct
     3721 attggccaca gttcttcttt tggcagctct tcccagaagt agtctccacg aagaacgttt
     3781 ttgccatccc tatcagtttg ctttgcttcg ccatcataaa cttccagttt aatcttctgg
     3841 aaacgtgtag cccatttacc ccagttttcc tggccgccgt cggagtatgc atagccaata
     3901 atcagttcgt ctttaagctg tacatggccg tttggtctct ttgaaacacc catgttgtgg
     3961 cagaactttg atattccggt tctgatggta tcgatatgtg taaagttgat accgtcattg
     4021 gattccatta tggctattcc actatctgtt gtaaatctgt ggatggttga aagagcaata
     4081 aacttgccta tatcttcaac gtagaataca tcgcaggagt cctgtccagg gccgtatttg
     4141 tatgctatac ccttgtattc caatgtttca ggccagttct cgctggttga atcggcaatg
     4201 gatacctttg taaagtctcc atccggacat ctccatgtat agtagcagta cagtgtgccg
     4261 tccaattcaa caaagctgat ttctccagca ccccaggctg aatctgcttc gtcaaagtaa
     4321 attattggag ctgggtctcc gccccatcct gaaccgttcc acttttcata tggtccgtca
     4381 gggttctttg aacgggcaac aaaaatgttg ttgtttatac cgccgttggt gctgactatt
     4441 gttgatgtat aaccaaggta ataccatccg tcaaaataga ttagaccagg gtcacatact
     4501 gaatagtgat ccattgaatc cggtgttggt tgtagaacaa caatttcatt ggaccatgta
     4561 gctccgcgat cgtcggagtg tttgtatgta aaccagtccc attctccact ggtaccaggg
     4621 gttgcaaacc atgcgtccat tgttccatct gggtagaaca atatactggg accatagcga
     4681 tatccccagt gtccagctag aggagcgaaa acgtcaaaac catcatcgat agtcttgaat
     4741 gttatatgct ggtcttctct tggttcaact ctaggcaatt catccctgaa aatacgggga
     4801 tcttccgtag gttcaggggt tttctcgtct tcgtttgaac cacctgtgca tgccgtaagt
     4861 actactatca tgcttagaca taaaaatgat aaaagaatta gtaatttttt cctactcata
     4921 gaacctctca cctcttcctg tttgctatca ttatactaaa aatggactga ttgtcaattg
     4981 atttttaatt ttaacgttcc atatttacaa actactaatg taggtataga atatatgaaa
     5041 gatattaagg aaaattcagg aaaggaaaat tggtgcttga gaatacttgt aacggtcaaa
     5101 tctacaagca gaaaaaggaa ctatataaca ggcaaggaaa ttattgtatc tgacaataac
     5161 agaacaatga aggatctttt gtgtgattta gcctgtcaat tgcacgatgc ctacataagc
     5221 aaaagcattg acgctgatat tgttgcagct cttacagaag aggagctaag tgacggacag
     5281 gagagcggca aggttggttt tggcagaagg tatggcgaag gagtcgtctc acgggaaaaa
     5341 gcgattgaaa ccatgcttct tgtttttgag gatggtcttt tcagagtgtt tttaaacaat
     5401 gaggaagttg ttgatattaa cgaggcgata aacattaagg acggggacaa tttgatactc
     5461 gtccgttttg tcatgctttc aggaagttgc ttttcgtatg ttttctaagg aggatttatg
     5521 tttttgacat cgtatcgtga aaaagaagct cagttctaca aggaaaaaga aaggagactc
     5581 cccaaatcca acgattatta ctcacagctt cataatgcca tactctatca aatgtattat
     5641 cactacaatc atgaaacatg gactgaagat aaggttttcg atttgatagg agaaaaacat
     5701 gaaagtttgt atggtctctt tgacggcgaa ggttttgcaa tactggagtt tttgtttgaa
     5761 aacaaggctt caatgattcg cgacatttgg aagagaacgt ttgattttgg ctaccagtca
     5821 ggttttgaga ggcgtccttt cagaacaaag catagtatcg gtctgcatta tcgcagaatg
     5881 atatcttttc tatcaggttt tgcatacctg tcagttctga attttaatct tgaagagtat
     5941 ttgaaagatc ctaataaaag tgaaaacaca tattttacat ataacggcag aaaagccatc
     6001 tcctacacta tggctcatga aatagactaa ggtaacaata acgtaatgaa gatgattgag
     6061 catatagtat atgaaaacac aggaagcttg aaccatacag ttctaaaagc gcttgcaatg
     6121 tcaaagtccg gatgggcaca tgagatcctt gaaaagctat tgcattctgc acaacttcag
     6181 gaaggtctca ggcagtcaat ttggaattga ttgacgaggg tgatgccgac aactttattt
     6241 atcttctcaa agtcatactg gacaacaaaa tggagaggtt ttcatcggtt gtcagggcgt
     6301 ttgatgtatg gaccggtttg cccgtatcag ccgaaaagtc caaaaccgca agatactatc
     6361 tggaagttat atatgactgt gttgcaaacc ggcacgaaga aaaatatctt gccagcccaa
     6421 atcctgtcga aaaccatatt gcacttatgt gcattggttt ttatgagctg caggatgtgt
     6481 acaaggtcct ggatgatttc ctgggtaata atgatccggc aaaacgcatt gcaggttggt
     6541 accttttgtc ccagaccgcc gatcctcagg cacaacttga gtattttatc aaatacaatg
     6601 atgaacggac tcctgatgta ctggcatata tggcagattc tttggtagcg aaacttaaat
     6661 acggtcatga attgatgttt gaaaagctat tgaaaatgat aaacgaaaca ccaataaaga
     6721 caaaaacagt ctcttatccc ggcatgcagg aacttaccag aaagtactcc caggaattca
     6781 tgtacaatgg ccttgttgat ctggcaaagt cagctggtgc cgggcttaaa gatagcatgt
     6841 ttgataccct gtgtgactat tatgaaagaa tacctgttga tacaaggatt gtatttgttg
     6901 agaatctttc caataacatg acgacaaaaa aacacaggca gacaatacta aatgctgcat
     6961 gcgacaagag taagtttgtg agaattattg cggcatcaaa gttgaaagaa atgaagcttt
     7021 ccgaggaaga atatatggag cttgaatcca tgcttcgtca taagacggag tctttacgaa
     7081 aaaacatcat ggtattgctg cttgaacaaa gtcccgagga ctgtctggag tctgtgagaa
     7141 gattgcacaa ttctcaagac gcaaactgca aactggcagc caggcagatg gttatggaca
     7201 tgcagtctga ttccagattt gcaaaggtat taagcaaggc caaagaaatt gtcagcatgg
     7261 aggaggttgt cgcggaaagc gatgagaatg tatatggttt ggttgattat ggcaagctaa
     7321 ccaaacttgc tgtgcccaaa aagaaaccat catacaaatt caaagatgca ttacctgacc
     7381 tgatggaact tgagaaaaag gtacataaat taactgaagt cgttaaaagg catgtaaatg
     7441 aggtatatta cagcgaaaat gaggagattg tctacggtag cgagaga
//
LOCUS       DAONVY010000034         7440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7514_length_7440_cov_3.119025, whole genome shotgun sequence.
ACCESSION   DAONVY010000034 DAONVY010000000
VERSION     DAONVY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_7440_cov_3.119025"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1321
                     /locus_tag="PLI11_01665"
     CDS             77..1321
                     /locus_tag="PLI11_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPZ51603.1"
                     /translation="MSRFFKRVTTTFLGLVLAIFMVLNTTPYSLKVEATDLMEGIESN
                     NVEGKTVDGRFVEFTSNFSIEMFKRSFDDENALVSPLSIMLALCMTANGADGGTLAQI
                     EKVLGDGTKIGELNRYLLSYAKSLPNEPKSKFKISNSIWFKEGFHVERDFLQTNADYY
                     NANCYRSPFDEQTLKDINNWVKHKTDGMIDSILDRISEDAVMYLINAIVFDAEWETVY
                     KESDIIKDDFTNLDGRKTRVDFMHSFEARFLKDDSATGFVKSYKNGGYSFVALLPNED
                     VSIAEYINGLTGEYFINTLENVKNQSVSAYIPKFSYEYEVGLNDILKDMGMPDAFDES
                     KADFSRMNPDVRLVIADVLHKTFINVDEKGTKAGAVTKVEMAPTSMPQGETVMLDRPF
                     VYAIVDNSTNLPLFIGVVTNLD"
     gene            complement(1349..4486)
                     /locus_tag="PLI11_01670"
     CDS             complement(1349..4486)
                     /locus_tag="PLI11_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ51604.1"
                     /translation="MLFKKTRRILVLCIFALLAGTAIVGCKSKYEDPSVTSNSKKHVY
                     TDLLDNIIITAFHPISYPELLTQEQFDLAADAGIDVMEYCGAMRVTDPASMKLALQFA
                     ANAGILCNVYDDRIQNNALSMSREELFAIFNEYKGRDGLGGYYILDEAVSPNDYAKLI
                     NEVIEFDPNSTPFFNNYPDIWNDGYYQDRLSDLAHLIINKDNFVLSFDNYPFGPAPNS
                     VDEYNLFKHFEAIRQAGNENNVRTGFYAQVISYGYRKLNRSETLYHVNVGLAYGCTEI
                     NYFAWGTPNEVLCPGFGDGVIDRNGNPTDLYYAIKEINRYAHTIGPTLVKLRAAEVYH
                     SGANSTNPVYQRIPQNFFVQSDSYIILSLMEHKITGRNYLMVVNKYMNAPQTIGLRFT
                     DVGSLSEVNQTTGNLTPIGNEYKNGVFTRTLDAGAAVLLALPEGVSFVTPKKIKESAN
                     LFENAYVLASDSRGSSGWFVNRVNDGITISEEHFMGWQAIRNEKEWLQFDLGEVKEFN
                     RLDLYPAGNGSASGVYFPSGVKISVSNDGKTWTTVKNIENIEQPTITVPTFRFNKASA
                     RYVRLDFVKRGNICELAEAKLYNDDGSIPMPDPTDYVDEEVAPGENIAKGKPVVDYSS
                     NYEHPEWNNSIHFVTDGNYKTNWASDVLLTNTQDSIEYLTIDLQRIYDIEKIILHPMY
                     DANTDGYTTIAFPIDFRIDISTDGTTFTTVAEYKGGITDTEPLTITFTPTKGRYVRVY
                     CTKLQQHPGALNAYMVQFSEIEVFTYMDELTGAKRTLQKAITEAKLIDLNFYVNSSRI
                     LLNEAISEAEQVLSTQSDNLEALLNAVAELNDAISKMIAIPLGDNIALGKPVTATSDY
                     VAPEGIFKASYLTDGINPVNLVPYQTHNGWSINVYDDIKRDTPVDIIIDLEQLETIAS
                     IMLQPANYDAKFYPTSYQIMTSPDGNNYQIVAVVENIEGVKPGDVLRYKVDNVLARYI
                     KIHITLHSPYTAGTPAYISQFGEIEVYSAVEYTEPDTNPTTNDSTNPFIWVVLVFGFC
                     CMFSIRNMQKRLLNK"
     gene            4667..5551
                     /locus_tag="PLI11_01675"
     CDS             4667..5551
                     /locus_tag="PLI11_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51605.1"
                     /translation="MKADKSKWYCKEVFSDNPYDYVIGFTETIFSHGIHIHDFFELNI
                     VLSGSAIHRFNANDFRIHIGDVYIIPPRVPHGYLETKDLKVFHILVHNLFLDKYSAEL
                     ANFFSYYMLFTIEPLLNEGSSEPYLLQLDKDQFDEIKYFLSKLEHFYLKFDTQSCIIR
                     DTLGLLVILRLCQFYQEKTIDGFDLSNIDRAYIEGVRYMFNNYNKKITVNQLAELVHL
                     SKNAFTIKFKKMFNETPGEFLISYRLTKAKNLIQYTDWPLSTIAEQTGFYDVSHFVKT
                     FKAREGITPDDYRRNCNN"
     gene            complement(5579..>7440)
                     /locus_tag="PLI11_01680"
     CDS             complement(5579..>7440)
                     /locus_tag="PLI11_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51606.1"
                     /translation="IITPESVKEIPGFLITLISDFFKRLFYIFRIVWETRKWILFLMV
                     FMALFNGVMPVAGAYISANLLNALAKAFLQQVDFKLVTFLLGLQFGYFILISIVNNIY
                     SMLNNIAGQLVVNHIKLKIMYKAKEVDMASFDSPEFYKKLENANREAGHRPIQVINST
                     FNIISTLITMISFITILWVISPFAPFIIILVSIPSAIINFIYRRKNFMYMRRRSKDRR
                     QMNYYSGLVVDKDLVKEVRMFNLSDFFIGCYKETFDKYFAGLKKLIRQEGFWHIGLSI
                     LSSVVNFFLFVFIARQVIAGKLQVGDYALYTGALNSISSGVAALIATTAGIFEGTLFI
                     DNMIEFMNEKRTIVPSTQEPGNPVRHVGHVIQFENVSFRYPGTERDVLKNINLTINAG
                     DTVVLVGLNGAGKTTLIKLLTRLYDPTEGRILLDGKDIREYDVDEYYKIFGVIFQDFG
                     KYAFKVNENISFGQIDKGFDSEGIYEAALKSDADSFIQKLPDKYETPLMRIFEENGIE
                     LSIGMWQKLAIARAFYSDSDILILDEPTASLDPMAEKAVYEQFDELRKGKTSIFVSHR
                     LSSTTLATKIVVLEYGEIIEEGSHRELMELKGRYYELFSTQASRYVESYTELD"
BASE COUNT         2087 a         1455 c         1542 g         2356 t
ORIGIN      
        1 tatccaatag agattttgaa ggtttaattg agctgattgg cagtatggag agtgacggat
       61 aaaaggagga atgcatatgt ctagattttt taaaagggtt acaaccactt tcctgggact
      121 tgttcttgcc atattcatgg tgctgaacac aacaccctac agtctcaagg tggaagctac
      181 agatttgatg gaaggtatag agtcgaataa tgtggaaggc aaaacagtgg atggaaggtt
      241 tgttgagttt acatcaaact tttcaattga gatgttcaaa aggtcttttg atgatgagaa
      301 tgcgctggtt tcgcctctct ccatcatgct ggcactttgc atgactgcaa acggtgctga
      361 cggaggtaca ctggcgcaga ttgaaaaagt tttgggtgat ggtacgaaaa taggagagtt
      421 gaacagatat ctcttaagct atgcaaagag tcttcccaac gagcccaaat ccaaatttaa
      481 aatttccaat tccatctggt tcaaggaagg ttttcatgta gaaagagatt ttctccaaac
      541 aaatgcagat tactataatg ccaactgcta cagatctcct tttgatgagc agaccctgaa
      601 ggatattaat aactgggtga agcacaagac tgatggcatg atagacagta tccttgacag
      661 aatttcagag gatgcagtca tgtatctcat taatgccatt gtgtttgatg cggaatggga
      721 aacggtttat aaggaaagtg acataatcaa ggatgacttt accaaccttg acggaaggaa
      781 gacaagagtt gattttatgc attcctttga ggcaaggttt ctaaaagatg atagcgcaac
      841 aggatttgtg aagtcttaca aaaacggggg atacagtttt gtggcacttc ttccaaacga
      901 agatgtgagc atagcggaat atatcaacgg attgacggga gaatatttca ttaacactct
      961 ggaaaatgtc aaaaatcaaa gtgtcagcgc atatatccca aaattcagct atgagtatga
     1021 ggttgggttg aatgatattt taaaggatat gggcatgcca gatgcttttg acgaatcaaa
     1081 agcggatttt agtaggatga acccggatgt aagacttgtt atagctgatg tcttgcataa
     1141 gacctttatc aatgtggatg aaaagggtac gaaagcaggt gcggtaacca aggtggaaat
     1201 ggctccaact tccatgcctc aaggtgaaac ggtaatgctt gaccgtccct ttgtatatgc
     1261 gattgttgac aacagcacca atttgccgtt attcattgga gtggttacaa atttggatta
     1321 aagaaaaggc accaggtggt gcctttaatc atttgtttaa caaccttttt tgcatattac
     1381 gtatagagaa catgcagcaa aaaccaaaga ccaacactac ccaaatgaaa ggattggtgc
     1441 tgtcgttggt tgttgggttt gtatcaggtt ccgtatactc aaccgcacta tagacttcaa
     1501 tttctccaaa ttgagagata tatgcagggg tgccagctgt ataagggctg tgaagagtta
     1561 tgtgaatctt tatatatctt gcaagtacat tatccacttt gtatctcagc acatctcctg
     1621 gctttactcc ttcaatgttc tcaaccactg caactatctg atagttgttg ccgtcaggcg
     1681 aagtcatgat ttggtagctt gtagggtaga acttcgcatc gtagttggca ggctgcagca
     1741 ttatggatgc tatggtttca agttgttcca ggtcaataat aatatccaca ggtgtatccc
     1801 tttttatatc atcataaaca ttaatgctcc atccattgtg tgtttgatag ggaaccaaat
     1861 taacgggatt tataccgtct gtaagatacg atgctttaaa tatgccttca ggcgctacat
     1921 agtcggatgt ggcagtgact ggtttgccaa gggcgatatt gtctcccaga ggaattgcaa
     1981 tcatcttgct tattgcatca ttcaattctg caacagcgtt aagaagggct tccagattgt
     2041 cgctctgtgt gctgagtact tgttctgctt cagaaatggc ctcgtttagt aaaatcctgc
     2101 tgctgttcac atagaagttc aggtcgatca gctttgcttc tgtaatagcc ttttgaaggg
     2161 ttctttttgc acctgtcagc tcatccatat atgtaaatac ttcaatttca gaaaactgca
     2221 ccatgtatgc attaagagct cctggatgct gctggagttt ggtacaataa acacgtacgt
     2281 atcgtccctt tgttggagta aatgtaatgg taaggggttc ggtgtctgta atgcctcctt
     2341 tatactctgc gacggttgta aatgtggtac cgtctgtgga tatgtcaatc ctgaaatcta
     2401 tggggaatgc aatggtggtg tatccatctg tgttggcgtc atacatagga tgaaggataa
     2461 tcttttctat gtcgtagatt ctctgcaggt caatggtaag atactctatg gagtcctgag
     2521 tatttgttag gagaacgtct gatgcccagt ttgttttgta gtttccatcg gttacgaaat
     2581 gtatgctgtt gttccactcg ggatgttcat agttgctgga ataatctaca acgggttttc
     2641 cttttgcgat attttcgcca ggtgcaactt cttcatcgac atagtctgtt ggatcaggca
     2701 tcggtatgct gccatcgtca ttatatagtt ttgcttcagc caattcgcag atattgcctc
     2761 tttttacaaa gtcaagacgc acatatcttg cgcttgcctt gttaaaacga aaagtgggaa
     2821 ctgttatggt aggctgctcg atattctcga tgtttttaac agtagtccat gtttttccgt
     2881 catttgatac tgatatttta actcctgagg ggaagtatac tccggaagca gatccattgc
     2941 ccgcaggata aaggtctaga cggttgaatt cctttacctc accaaggtca aactgcagcc
     3001 attctttttc gtttctgatt gcctgccagc ccatgaaatg ttcttccgag atggttattc
     3061 cgtcatttac gcgatttaca aaccaaccac tgctgcctcg tgaatcagaa gcaagaacat
     3121 atgcattttc aaacagattt gcactttctt tgatcttttt gggagtaaca aaagaaactc
     3181 cttctggcag tgccagcaat acggcagcac cggcatccaa agtacgggtg aatacaccat
     3241 ttttatactc gttccctatt ggtgtcaaat tgcctgtagt ctgatttacc tcactcagcg
     3301 agccaacatc agtaaatctt aatccaatgg tttgaggagc gttcatgtac ttgtttacta
     3361 ccattagata gtttcttcct gtgattttgt gctccattag cgacagtatt atgtagctgt
     3421 ctgactgtac gaagaaattc tggggaatgc gctgatacac tggatttgtc gaatttgcac
     3481 cgctgtggta aacttcagca gctctcagtt tgaccagtgt tggccctatt gtatgagcat
     3541 acctgttgat ttcttttatg gcatagtaca ggtcggtggg attgccattt ctatcaataa
     3601 ctccatcccc aaaaccagga cacagtactt cgttgggtgt accccatgcg aaatagttga
     3661 tttccgtgca tccgtaagcc aagcccacat ttacatggta aagagtctcg cttctattca
     3721 gttttcgata accataggaa ataacttggg cgtaaaaacc ggttctgaca ttgttttcat
     3781 tgccagcctg tctgatggct tcgaaatgct tgaaaaggtt gtactcatcc acagaatttg
     3841 gtgcaggacc gaaaggatag ttgtcaaagg aaaggacaaa gttgtctttg tttatgatta
     3901 agtgggcaag atccgatagg cggtcctggt aatatccatc gttccaaata tctggatagt
     3961 tgttgaaaaa tggggtggag ttcggatcaa actcaatcac ttcatttatt agttttgcat
     4021 aatcattagg agatacagcc tcatccagga tatagtagcc gccaagacca tccctccctt
     4081 tatactcatt aaaaatggca aaaagctctt cccggctcat gctcaaggcg ttgttctgta
     4141 tacgatcgtc ataaacattg cagagaatgc ccgcatttgc tgcaaattga agggccaatt
     4201 tcattgatgc cggatctgta acccgcattg cgccgcagta ttccataacg tctatacctg
     4261 catcagcagc caaatcaaac tgttcctgag ttaataattc aggatatgag atgggatgaa
     4321 atgcggtaat gataatgttg tccaacaagt cggtatagac atgcttcttt gagtttgaag
     4381 tcactgaagg gtcttcatat ttgcttttgc agccaacaat tgcagtgcct gccagcaagg
     4441 caaatatgca taaaaccagt attcgtcgtg tttttttgaa taacatacat attccctcct
     4501 ttgtagtcat catattcatt ctagtttctg gttttgcctc attccatgta tatataacac
     4561 ttttttgatg taatattgaa cattatctgt ttgcttttgg tgttcttgga tagtaaaata
     4621 aatgtgatat aggtgtgatg ttggaaagga gaatattgat gcaattatga aggctgacaa
     4681 atcaaaatgg tattgcaaag aagtatttag tgataatcca tatgattatg taatagggtt
     4741 tactgaaacg attttttcac atggaattca tattcatgat ttctttgaac tcaatattgt
     4801 tctgtccggg agtgcaattc atcgtttcaa tgccaatgat ttcaggatac acatagggga
     4861 cgtttatatc attcctccca gagtccctca tggctacctt gaaacgaaag atttgaaagt
     4921 attccacatt cttgtacata acctgtttct tgacaaatac tctgccgaac ttgccaactt
     4981 cttcagctat tatatgttat tcaccataga accgctactt aacgaaggaa gcagcgagcc
     5041 atatcttctc cagttagaca aggaccaatt cgatgaaatc aaatactttc taagcaaatt
     5101 ggaacatttc tacctcaaat tcgacacaca aagctgtata atcagagaca ccctgggttt
     5161 gctcgtgatt cttaggctgt gtcagtttta tcaggaaaag acgatagatg gttttgattt
     5221 gtcaaacata gacagggcat atattgaagg tgttcggtac atgtttaaca attataacaa
     5281 aaaaataact gttaaccagc ttgctgaact cgttcatctt tccaaaaatg cctttacaat
     5341 caaattcaaa aaaatgttta atgaaacccc aggtgaattc cttatttcct acagactcac
     5401 caaggcgaaa aatcttatcc agtatacaga ttggccgcta tccacaatcg cagagcaaac
     5461 cggtttctac gatgtatctc actttgtaaa gactttcaaa gctcgtgaag gaatcactcc
     5521 cgatgattat aggcgtaatt gcaataattg acaatagtac caaccttcct ttttattgtt
     5581 aatctaattc tgtataactt tccacatatc tgctggcttg tgttgaaaac agctcataat
     5641 atctgccttt gagttccatg agctctctgt gggaaccttc ctctatgatt tctccatact
     5701 ctagcacgac gatctttgta gcaagggtgg tgcttgataa tctgtgggaa acaaagatgg
     5761 aggttttgcc ttttctgagt tcatcaaact gttcatagac tgccttttcc gccatggggt
     5821 caagagaagc ggtgggctcg tccagtatca gaatatctga gtcgctgtaa aaagctctag
     5881 cgatcgcaag tttctgccac attccaattg aaagttcaat gccgttctcc tcaaatatcc
     5941 tcatcaaagg cgtttcatac ttgtctggta gtttttgaat gaaagaatcc gcatcactct
     6001 ttaatgccgc ttcatatatt ccctcggaat caaagccttt gtctatttgt ccaaaagata
     6061 tgttttcatt tactttgaaa gcatattttc caaagtcctg aaatatgacg ccaaagatct
     6121 tgtagtactc gtccacgtca tactctctta tgtcttttcc atcaagaaga attcttcctt
     6181 ctgtcgggtc atacagtctt gtcagaagtt ttataagggt ggttttgcca gcaccattca
     6241 atcctaccaa aacaaccgta tcgcctgcgt ttattgtaag gttgatgttc tttaatacat
     6301 ccctttctgt accgggatac ctgaagctta cattttcaaa ttgaattaca tgtcctacat
     6361 gtcttacagg atttccaggt tcttgtgttg atggcacgat ggtcctcttt tcattcatga
     6421 actcaatcat gttgtctatg aacaaggtcc cttcaaaaat acctgcagtg gtggctataa
     6481 gggcggcgac acctgaagat attgagttta gtgctcctgt gtacagggca tagtcaccaa
     6541 cctgaagttt tcctgctatg acttgccttg caatgaacac aaacagaaag aagtttacta
     6601 cgctggagag tattgaaagt cctatgtgcc aaaacccttc ctgtctgatt agctttttta
     6661 gtcctgcaaa gtatttgtcg aaggtttcct tatagcatcc tatgaaaaaa tccgacaggt
     6721 taaacattct tacttccttg accagatcct tgtcaactac aagacctgaa taatagttca
     6781 tctgccttct gtcttttgac cttcttctca tatacatgaa gttcttcctt ctgtaaataa
     6841 agttaatgat tgcagatgga attgatacaa ggataattat gaatggggca aaggggctga
     6901 tgacccacag tatggttatg aaggatatca tggtgattaa tgtactgatt atgttgaatg
     6961 ttgaattaat aacctgtatg ggtctgtgac cggcttctct gttggcattt tccagcttct
     7021 tgtagaattc gggtgaatca aagcttgcca tgtctacttc ttttgccttg tacataatct
     7081 tgagttttat gtgattcaca accaattggc ctgcaatgtt gttcagcatt gaatatatat
     7141 tgttgactat gcttataagt ataaagtagc cgaactgcag tcccagcagg aaggtgacaa
     7201 gtttgaaatc cacctgctgc aaaaatgcct ttgcaagtgc atttaataga tttgcgctta
     7261 tatatgcgcc tgcaacgggc atgacaccat tgaacaaggc catgaatacc attaagaaca
     7321 gtatccattt tcttgtctcc cagacaattc tgaatatgta aaaaagcctt ttaaagaaat
     7381 cgctgattag tgttattaga aatcctggaa tctcttttac ggattctggt gtaattatat
//
LOCUS       DAONVY010000035         7400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7556_length_7400_cov_3.615929, whole genome shotgun sequence.
ACCESSION   DAONVY010000035 DAONVY010000000
VERSION     DAONVY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7556_length_7400_cov_3.615929"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLI11_01685"
     CDS             complement(<1..675)
                     /locus_tag="PLI11_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPZ51607.1"
                     /translation="MSKQVAVIDIGTTGIRILVAKINETGIPHIIAKSQVSSNARKKF
                     EIEDESLLTDSIKSALKKIRDQTGIIIKTAYISISGDCLKFLSNSDSIDISDNEGDIT
                     SAHVGQVLDKTASVEIYDDELLVDIIPLTYYVDGKPENDPLGLVGNKLSVEAKVVLGS
                     KEYIEKLTACVNNAGLQVDGYVPFFMAMGELLSESEVDEEEQSTLLVDVGGQVTEYVI
                     YYKGKPF"
     gene            complement(802..1146)
                     /locus_tag="PLI11_01690"
     CDS             complement(802..1146)
                     /locus_tag="PLI11_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HPZ51608.1"
                     /translation="MIPILGLILGLIIGILMPFSIPSEYSTYVAVGILAVFDTVFGGV
                     VAVLQGRFNIKVFLSGFFGNSLLAMALAYIGDQMGLPLYMAAIFAFGNRLFDNFATIR
                     RLTLDKYTKKGK"
     gene            complement(1224..2000)
                     /locus_tag="PLI11_01695"
     CDS             complement(1224..2000)
                     /locus_tag="PLI11_01695"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPZ51609.1"
                     /translation="MNRKGVRIIVILILFISVVIAFFFSPLFNIKEIEIMPMEYYDEK
                     EVYVHLAGLIGKNGFSEILKHKSLSQSESILSFQLDELEDKLVFECPYLRDVSISYRF
                     PNKVVVNTKERIPIFLLKERSVYLYIDSEGYLLSTFTEEDEKPNLPIVQGIELDSYKV
                     GQPITKSEDKRVDTAIKICNLLKQLSIHEGRIDIIDVSDSDDIWFYTHPSLSVKFGDM
                     EEPGLKLSYLKEILNAGFNGESDGIIDFSGGGYPVFRHNE"
     gene            complement(2073..3020)
                     /locus_tag="PLI11_01700"
                     /pseudo
     CDS             complement(2073..3020)
                     /locus_tag="PLI11_01700"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
     gene            complement(3046..4158)
                     /locus_tag="PLI11_01705"
     CDS             complement(3046..4158)
                     /locus_tag="PLI11_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51610.1"
                     /translation="MSFGSRRNIFLGANTRYGFKSLYETLLTDYDNYNLTVYILKGGP
                     GTGKSTFVKKVIGLLDSLGCNMDIVHCSGDPQSLDGVISTEGGIAVVDGTSPHMIDPK
                     CPGAREEIINLGSFWDTSFLIRYRDDIIQFMKDKSDYYKRAYHYLQAAGIIYDDTVNI
                     MEESFEKNKAYLFASNLADEILGDFEPSLSLGKDKKLFATAITPAGLMGSVNEIFRDD
                     KVYSIDTTMGMSTSSILEVIKVRANLCGFNTESFLCGFDTKLEHIRIPELKTAVVSSN
                     PYHEASGSDMYYEYPLEGFLSEYNVLRAKDDIKYNSIRFDELLNKAVRMMSKSKREHA
                     KLEEIYISSMDFDELNSYLEDVIKEIEGVIQMHLSN"
     gene            complement(4207..5823)
                     /locus_tag="PLI11_01710"
     CDS             complement(4207..5823)
                     /locus_tag="PLI11_01710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPZ51611.1"
                     /translation="MRLSDSQKKAVYHREGPCLVLAGPGSGKTTVITRRIGFLTDGIT
                     GSDWLLSVTFTRAAGREMAVRYARLYPGRDLPHFGTVHSLCNRIIYDYENITGNRFHR
                     IGGDAKVKQKILEDIWLKVNKGVNMPESDEVFSLISRRTNRPEYSSDGFIRNFDKIYD
                     MYNAYKRQNNLVDFDDMVLFAKELLENDERIRRRWSKKYEYIQVDEGQDLSRAQFEVM
                     KYLAGHGNIFVVADDDQGIYAFRGADPGCVLEFEKHYSNCVKYYLEENYRSCKRIVQC
                     ASSVIALNRNRYEKNLFTNNRLGEKIEFIHLRNLSEQARFVCEKISKINKRGSVCVIY
                     RNNLSAVMFKLYLNHFNLPYNVLGGNIDIASDFMVKKVLARIRDAEKEARFLIPRPKK
                     VFERMLLSGLYDDMSNYYERHGKSRMFAEYVYEFLKTLCSINGSYEELIKCLEFRADE
                     DANITLSTAHSAKGLEFDTVFIVDLVNGEFPKRERMSGGMLEEERRLFYVAMTRAKNK
                     LYLVYPEMRGSNQEEASVFYVETLKHACNK"
     gene            5943..6386
                     /locus_tag="PLI11_01715"
     CDS             5943..6386
                     /locus_tag="PLI11_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51612.1"
                     /translation="MKKRFFAVLITVALVTVCLFSQVSAQTKFSEYDFSGWTEIKSYF
                     EDYQSFVFPDWRHPFKETDTDLIPEGWKYEKPAGAADSEIVNESGDIGFFPSPNSELY
                     TKDAISNPYVWVMRTRLSAGKVAQYIVRATHEKINNVRFTDPDGT"
     gene            6417..6938
                     /locus_tag="PLI11_01720"
     CDS             6417..6938
                     /locus_tag="PLI11_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51613.1"
                     /translation="MEKDVLGSSGISFRPHGTSVYIFIKQYDPSAPKKLNNKVVKIDD
                     VANFIFPRGEYQDVAVVDDGKSVTIYFGDELVAKISFSEKKKYDISNEEFYSKATVTG
                     KNGSVLANVDNALISTKSVLCIAANDIIGVGEIGYTEYKIESNPQTGDTAAFIGLVAL
                     LGMAVLKKRPIEE"
     gene            7071..7156
                     /locus_tag="PLI11_01725"
     tRNA            7071..7156
                     /locus_tag="PLI11_01725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7105..7107,aa:Leu,seq:gag)
BASE COUNT         2170 a         1686 c         1242 g         2302 t
ORIGIN      
        1 aaacggtttt cccttgtagt aaatgacgta ttcagtaacc tgcccaccta cgtctaccaa
       61 tagagtgctt tgttcttcct catctacttc agactcggac aacagctctc ccatggccat
      121 aaaaaatgga acataaccat ccacctgaag accagcattg ttaacacagg cagtgagttt
      181 ttcaatatat tcctttgaac caagaacaac ttttgcctct acacttaatt tgtttcccac
      241 aagtcccaat gggtcgtttt caggctttcc atctacataa tatgttaacg ggatgatatc
      301 gacgagcagc tcatcatcgt aaatttccac acttgcggtt ttgtcaagaa cctgtccaac
      361 atgagcagat gtaatatcgc cttcattatc tgaaatatct attgagtctg aattacttag
      421 gaatttaaga caatcacctg aaatacttat atatgcggtt ttaataatta ttcctgtttg
      481 atccctgatt ttcttcaacg ctgatttaat tgaatcagta agtagacttt catcttcgat
      541 ttcaaacttt tttcttgcgt ttgacgaaac ttgggatttt gcaattatat gtggtattcc
      601 tgtttcatta attttggcaa caagtattct tatgcctgtg gtaccaatat ctatgacagc
      661 tacttgcttg ctcatcgtat caacactcct taacaatcac aacaattata acctgtattg
      721 gaagagacag tttacgacaa ttgaatgctc agcgataatt atacataatt taaacacttt
      781 tttcaacaat caatacaact attatttgcc ttttttcgta tatttatcta atgtcagcct
      841 ccttattgtg gcaaagttgt caaacaacct gtttccgaat gcaaaaattg cagccatgta
      901 taaaggaagt cccatctgat cacctatata tgcaagggcc attgccagaa gagagttgcc
      961 aaagaagcct gacaaaaaca ccttgatatt gaacctgccc tgcagcaccg caacaacccc
     1021 cccaaataca gtatcgaata ctgccagaat acctactgct acgtatgttg aatattcaga
     1081 cggtatgctg aacggcatga gtattccaat tataaggccc aatataagtc ctaagatcgg
     1141 tatcattttt tctcctctca tcccatggaa aatatatatt ttctttatta tacagaatta
     1201 tataaataaa ataaacccct ttttcattca ttatgcctaa aaactggata acctccaccc
     1261 gaaaagtcaa ttataccgtc cgattcacca ttaaaaccag cattaagtat ctctttaaga
     1321 taagaaagct tcaaacctgg ctcctccatg tccccaaact taacagaaag agacggatgg
     1381 gtataaaacc agatatcgtc cgaatcactt acatcaataa tatctatcct gccttcgtga
     1441 atagacagct gtttaagcag gttgcatatc tttatagcgg tatcaactct tttgtcctcc
     1501 gattttgtaa tgggctgccc tactttgtaa ctatcaagct ctatcccctg cactatgggc
     1561 agatttggtt tctcatcctc ttcagtaaaa gttgaaagca aatatccttc cgaatcaata
     1621 taaaggtaca cgctgcgttc ttttaggaga aatatgggga ttctttcctt tgtattcacc
     1681 acaaccttgt ttggaaaacg ataacttata ctcacatctc ttaaataagg gcattcaaaa
     1741 acaagcttgt cttcaagctc gtccaattgg aaggataaaa tactttccga ctgggacaaa
     1801 gacttgtgct tgagaatctc cgaaaatccg ttcttaccaa ttaaaccagc aagatgcacg
     1861 taaacctcct tctcgtcgta gtattccatt ggcatgattt caatctcctt tatattaaac
     1921 aaaggcgaaa agaagaaagc aataactacg gatatgaata atatgaggat tacaataatc
     1981 ctgacacctt tgcgattcaa aaagctaccg cctttcccaa aaaaatttat cgccaatatt
     2041 atatcacaaa ttccatagta ttgcttttga tttcacacgg ttcttgctgc acaaagtttt
     2101 acagtcttca ctctgccgcc cagcatctga aacttctttt cgatgtcggc atatcctctt
     2161 tctataaagt ggtcgtcatc aatatatgtg tcgccaactg cgcaaagtcc tgctatcaga
     2221 agtgacgctc cacatcttaa gtcagatgat accaaatcgc aaccaacaag acgttcaact
     2281 ccgttgatga gtatgctttt cttgccaata tactcaatat cggcacccat tttaaccagt
     2341 tcaggcacat gtttgaatct gttttcaaaa attgtttcct caacaacact agctccagac
     2401 gttacactca aaagcgaagt gagaggcgct tgcaaatccg ttggaaaccc gggatacggc
     2461 agcgtcctga cactgattcc acctaatgta tcaagttttc tttttgtaga ttcgacatag
     2521 actgcgttgt ggtaaatttc aattttacat ccaatattgt agagtaactc gataagggaa
     2581 aaaacatgag acggtacaat gtttcgtaca aacactttgc cgcctgtcgc cgcactggct
     2641 atcatatatg tgccagcttc gattctgtca ggcataatgc tgtgctcttc acagtcatgc
     2701 aattccttta cgccttcgac aactatttcg cctgtacctg cgcctttgac ttttccacca
     2761 attgcgttaa ggtacttttg caaatcttca atttctggtt ctcttgcact atttttaata
     2821 actgtcgtac cctctgccag cactgcagcc agcatggcgt tttcagtagc ccctacactg
     2881 ggaaaaggca gttttatttc attgccttta agttttgcag ccctggcata tattatatcg
     2941 ccctcttcaa tcacagcacc cagagctctt agcgcattca aatgcatatc tataggcctt
     3001 tctcctatag tgcagcctcc tgggtgcgtt accccattga attattcaat tagagagatg
     3061 catctgtatg acgccttcga tttcttttat aacatcttca agataagaat taagttcgtc
     3121 aaaatccatt gaagatatat agatttcttc gagttttgcg tgttcacgct tggatttgga
     3181 catcattctt actgctttgt tgaggagttc gtcaaaccta atgctgttgt atttgatgtc
     3241 atcctttgca cggagcacgt tatattcgct caaaaatccc tcaaggggat attcatagta
     3301 catgtcgctt ccagaggctt cgtggtatgg atttgacgac actactgccg tcttcagctc
     3361 gggtatccta atatgctcaa gctttgtgtc aaaaccgcaa aggaaactct ccgtgttgaa
     3421 accgcagaga ttcgccctaa ccttgatgac ttccaatatt gaggaagtac tcatacccat
     3481 cgtggtatct attgagtata ccttgtcgtc cctgaaaatt tcattgacag agcccatcaa
     3541 acctgcaggc gttattgcag ttgcaaagag ttttttgtcc tttccaaggc ttaatgacgg
     3601 ttcaaagtct cctagtatct catctgccag gtttgatgca aagaggtatg ccttgttctt
     3661 ttcgaaagac tcttccatta tattgactgt atcatcatat atgatgccag ctgcttgaag
     3721 ataatggtag gcacgtttgt aatagtcgct tttgtccttc atgaactgaa taatgtcatc
     3781 cctgtaccgg ataaggaagg atgtatccca aaaagacccc aaattaataa tctcttccct
     3841 tgcaccagga catttgggat caatcatatg agggcttgtg ccatccacaa ctgcaatccc
     3901 gccttcagta ctgataaccc cgtccaggct ttgggggtca ccggaacaat gaacaatgtc
     3961 catgttgcag ccaagactat caagaagacc aatgactttt tttacaaagg tggacttgcc
     4021 cgtaccaggt cctcctttaa gaatgtacac tgttagattg taattgtcgt agtccgtcaa
     4081 caatgtttcg tacaaacttt taaacccata tcttgtgttt gctcctagaa atatgttcct
     4141 tctactgcca aaactcatat tactgctcct tgtaatcctt acacattata ttatgacctc
     4201 cctatactat ttgttacatg catgctttaa cgtttccaca taaaacacac ttgcctcttc
     4261 ctgattgctt ccccgcattt caggataaac caaatacaat ttgttttttg cccttgtcat
     4321 ggctacataa aacaaccttc tttcttcttc caacatgccg ccactcattc tttccctctt
     4381 gggaaactcg ccattcacca aatcaacaat gaaaaccgta tcaaattcca gtccttttgc
     4441 agaatgggca gttgacaaag taatattggc atcttcgtca gccctaaact ccagacactt
     4501 gattagttcc tcatacgaac cattaatact acataaagtt ttcaaaaatt cataaacata
     4561 ttcagcaaac atgcgacttt ttccatgtct ttcgtaataa ttcgacatat catcatataa
     4621 tcctgaaaga agcatcctct caaaaacctt tttgggtcta ggtatcaaaa accttgcttc
     4681 tttttccgca tcccttattc ttgccaaaac ttttttaacc ataaaatcag acgcaatatc
     4741 aatatttcca ccaagtacgt tgtatgggag attgaaatga ttcaaataca atttgaacat
     4801 tactgcagac aaattgtttc tgtaaatgac gcagacactc cccctcttat tgatctttga
     4861 aatcttctca catacgaacc ttgcctgctc acttaaattt cttaagtgta tgaactcaat
     4921 cttttccccc aacctattat tcgtaaacaa gttcttctca tacctgtttc tattcaaagc
     4981 aatcacactg ctggcacact gtactatcct tttgcatgaa cggtagtttt cttccaggta
     5041 gtacttcacg caattggaat aatgtttctc aaactccaaa acacacccgg ggtccgctcc
     5101 cctgaatgca tatattccct ggtcgtcgtc cgctacaaca aatatgtttc catgtcctgc
     5161 aagatatttc attacctcaa actgagccct tgacaaatcc tgtccttcat ccacctgtat
     5221 gtactcatac ttttttgacc atcttctccg tattctttca tcattttcga gaagctcttt
     5281 tgcaaacaaa accatgtcgt caaaatcaac taggttgttt tgtcttttgt atgcattata
     5341 catatcatat atcttgtcaa aattcctgat aaaaccgtca gaggaatact caggcctgtt
     5401 tgtccttctg ctgattaaag agaacacctc atctgattct ggcatgttta ctcctttgtt
     5461 tacctttaac caaatatcct ccagaatctt ttgtttgacc tttgcatctc cacctatcct
     5521 gtggaaccta ttccctgtta tattctcata atcatatatt atcctgttgc acaaactgtg
     5581 caccgtgcca aagtgcggca aatctctgcc aggataaagc cttgcatacc ttactgccat
     5641 ttctctccct gccgctcttg taaatgttac ggacaaaagc cagtcagaac cggtaattcc
     5701 atccgtcaaa aacccaatcc ttctagttat aaccgtggtt ttgccgctcc ctggtcctgc
     5761 cagtaccaga caaggccctt ccctatgata cacagctttt ttctgagaat cgcttaatct
     5821 catgttactc ctttttctat atagtctacc gcttttttca tgtgggttca actcattttt
     5881 tatgcttgac aatttgaaaa gattaatata tttacaatat tatacaacag gaggaagtac
     5941 ttatgaagaa aagatttttt gccgtcctaa ttacagttgc acttgtaact gtttgtctgt
     6001 tttctcaagt aagtgcacag accaaattct ctgaatatga cttttcaggc tggactgaaa
     6061 taaagtcata tttcgaagac tatcaatctt ttgtgtttcc ggattggagg catcctttca
     6121 aggaaacaga cactgatcta atcccagaag gctggaaata tgaaaaacca gccggagcag
     6181 cagatagtga aatcgtaaat gaaagcggag atatcgggtt cttcccatct cccaactcag
     6241 aactctatac aaaagatgca atatcaaatc catacgtatg ggttatgaga acaagactct
     6301 ctgcaggcaa ggttgctcag tatattgtaa gagcaacaca cgaaaaaata aacaatgtaa
     6361 ggtttacaga tcctgacggt acatagatta cctataagga cgacgatggc aatgatgtgg
     6421 aaaaggatgt gcttggttca agcggaatat ccttcagacc acacggcact tcagtctaca
     6481 tattcatcaa gcagtatgac ccaagtgctc caaagaaact gaacaacaag gttgtaaaaa
     6541 ttgacgatgt ggccaacttc atattcccaa gaggagagta ccaggacgtt gcggtagttg
     6601 acgacggaaa atccgtcacc atctactttg gagacgaact tgttgctaaa atatccttct
     6661 ccgaaaagaa gaagtatgat atcagcaatg aggaattcta ttcaaaggca actgttactg
     6721 gaaaaaatgg ctccgttctt gccaatgtgg acaatgcgtt aattagcaca aagagtgttc
     6781 tttgcattgc tgcaaatgat atcataggtg ttggcgagat tggatacact gagtacaaaa
     6841 ttgaatcaaa cccacaaact ggcgacactg cagcctttat tggacttgta gcactgctgg
     6901 gaatggcggt tcttaagaaa agacctatag aagaataaca agtgatacac aaccgccggt
     6961 ctagccggcg gtttttgaaa atatgtcata agataaacat ataaaaaatt cagaaatggt
     7021 attgacaatt cgaaatcaac ttagtataat aatgattgtg ccaaggacat gcgattgtgg
     7081 cggaactggc agacgcgcac gtttgagggg cgtgtgggca acccccgtac gagttcaagt
     7141 ctcgtcaatc gcaccagata aatagaagtc agctgtgttg cagttgactt ctttttttat
     7201 gcattatata ctatatacat atatttcttc cgacgaggag actttgacat gtcgcaggaa
     7261 tatgttttcg aattttacgg aacaaaagaa gactttttga gttgtttgga ccgtttatcc
     7321 aacaattatt atcaaagttc caaaatctat tatatcaaca actatattgt gaagtttgta
     7381 gataatgaaa ttcacttcgg
//
LOCUS       DAONVY010000036         7371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7599_length_7371_cov_3.736468, whole genome shotgun sequence.
ACCESSION   DAONVY010000036 DAONVY010000000
VERSION     DAONVY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7599_length_7371_cov_3.736468"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..501
                     /locus_tag="PLI11_01730"
     CDS             76..501
                     /locus_tag="PLI11_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51614.1"
                     /translation="MGRERERENRSYTYLIVGLIVAILTFAFLLAAFGIVLNGNISVR
                     NLISYIVTAISFGVISGTFHYFRLFVAFWLFILGILVGFADMFRVFLVNDFGWNDLVG
                     LMAFFSYVVIGFFAGLFFQAIHYLYKLYKAKRQRPPEIK"
     gene            complement(546..2255)
                     /locus_tag="PLI11_01735"
     CDS             complement(546..2255)
                     /locus_tag="PLI11_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ51615.1"
                     /translation="MQMDIVPYGIRKKEIFKKTEEDKRVRRFMGVQKVLWKSEGDKCL
                     VTGEKVLLEDRDPQINLGQTEACVLENNGETASILLDFGVEFHGYVKLFVWSVSPEKV
                     RLRIRFGESAMEAMSDLGGEKNATNDHINRDQIIETGFLSMPEIGPSGFRFCRIDLID
                     PNTKISFKTVKGIFTYRELEYKGSFRCNDERLNEIWGVGAYTVHLNMQEYVWDGIKRD
                     RLVWIGDMHPETSTIQAVFGYDESVPKSLDLARDETPLPGFMSGISSYSLWWVLIHHG
                     WYMQNGDKEYLEEQRDYLKSLLIQFSQYIDEEGREQLPPTRFLDWPSHGNDEAFHAGL
                     HSLLRLSLLRGAELLEELGENETAAMCRERADILLKHVPDPNHNKQAAALMALADIAP
                     AEEMNDKVIKVGGARNLSTFFGYYILQAQALANDMVGALDNIRTYWGGMLDRGATTFW
                     EDFNIEWLEGSGRIDELVPEGVKDLHGDFGNFCYKGFRHSLCHGWASGPTAFMSGYVL
                     GIQVLKPGCEEIRIKPDLGDLEWAEGTYPTPMGIIWVRHEKNQDGTIKTEYKVPEGVK
                     VVE"
     gene            complement(2393..3343)
                     /locus_tag="PLI11_01740"
     CDS             complement(2393..3343)
                     /locus_tag="PLI11_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51616.1"
                     /translation="MYKRLFAFCVIFVMIFSLMAANAAKLSDLDMSKIELVKESKIDV
                     ESMSVGAEFGNPPAGWTYQFVLGEQPKIAEADGNKYIASNGFWQMFHEPVEEKYYMFS
                     IDVKVSGKQNFGIFARAGKEKFQYELNMSDFFEQDRSGEHDGLEALGGSGMYVRVEGK
                     KVTLYIKLYDESQKCDINNKLYAFETNYDFSTGFHTITLADDGKNVYVAVDDEIIARV
                     KFSDPKTYPIGEEQYYSKVVVYGGDGSELGTHDNALFAVNSVVAIGNRNSPTNFDNIS
                     LKTYTDKDDNPQTSDSGVALFALTVAFGMAVVVLKKRVFA"
     gene            3553..4857
                     /locus_tag="PLI11_01745"
     CDS             3553..4857
                     /locus_tag="PLI11_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPZ51617.1"
                     /translation="MQRNRSNWTIKKIIFLFIILSLIGKLIFDFSGFWLNFAFVFQYI
                     GYILGYLLFGFIIAYILNAYIDFWDKKLFKDLLNNNKKLKKALCMIIAYMTFFGILSF
                     LIFTLVPALTTTLKDFAVKIPTLIDKVVAYYNNLLDGTKYELSEDVLKSVTNALDSLL
                     KSATDAININLITSIVSNATSLLFNFIMGIMVSVYMLIEKEHTLVAINRVIDGLFRKR
                     TGERLRWIGHEVNQIFRKYFTGKLLQALICTILAYVAFSIARLPYAILFAVTFGITNM
                     IPYIGPWIGAIPIIIISLAYDFWLGVAAAICILIIQAVDNWFISPKIVGGKMGISPLL
                     VLAGLGIGGRLFGLPGLIFGDVLAAIFKLFFYDVYIRKRIEAKQLISPVNEIQEEKED
                     AESEVVEEEVVTETEETVSIKTKMETRWVSIKEKLRKVFKRK"
     gene            complement(4858..5667)
                     /locus_tag="PLI11_01750"
     CDS             complement(4858..5667)
                     /locus_tag="PLI11_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPZ51618.1"
                     /translation="MDVPIKGINSEEVKLARDLSTGKKVDNEQLFICEGLWAVEKIIK
                     KRIKVEMFFFCPDMIKTEQDKKKTNLMIEYCKDSYQISQKACRKISDRDGADGFFIIC
                     HMPRYDIEKLPLKDDMLVMVLDGLEQPGNVGATIRSLDAAGGDFAIMTNRRVRLTHSR
                     VIRASLGAAFMMPVCVCDMAEVVKWLVKNKFKIIVTDLTAKKSYYDIDYSGRVCIVAG
                     NEYLGISPDWRHVEGAIPCIIPMLGSVESLNVGFASTIVAYEARRYKSFIN"
     gene            complement(5738..6289)
                     /locus_tag="PLI11_01755"
     CDS             complement(5738..6289)
                     /locus_tag="PLI11_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPZ51619.1"
                     /translation="MKILVLYNTVDGNTQAVAEAIANALDADLVKIVRVKGVRAYGFF
                     TKLKHRMEVVRKKKPDIFPLRKDPRDYDLVFIGTPVWYDSYNPVYNTLFDVIKIYDRK
                     IALFSTGEEEDTKALDKLADYLVDSVLLGKFHCVEPIWCDTDEETQEILDKAVAWAKN
                     IAQEAQQMKEKEELERRKTIYKY"
     gene            complement(6406..>7371)
                     /locus_tag="PLI11_01760"
     CDS             complement(6406..>7371)
                     /locus_tag="PLI11_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HPZ51620.1"
                     /translation="PVKQLEPEMIWASSMPKVEFRETGKGLVEIEGHNFVYRFDVEKG
                     QFDSLELDGVELLSCPTAFNIWRAPTDNDRHVRNAWQWEEMHNAKEKLYDLKISSINP
                     TFISITASYSLAGPSRLPVVKYSVMWTVYGSGEIGVSVAAEVRKNIKSLPRFGMMLVM
                     PKGFEKVTYFGRGPVSSYADMKNASKLGLFNSTVTDEYTKYIFPQETGNHIDCRFAAV
                     YDLEGRGLLFKGMPKFNFSSLHYTPKMLDNTPHDKDLVPLEETVIHIDYKQNGIGSHS
                     CGPELNRKYEFNEKEFVYSFVIKPIIVYGDNIVRESRKLPSMYNF"
BASE COUNT         2098 a         1660 c         1298 g         2315 t
ORIGIN      
        1 tgcattattg cagccctttt tttgttggaa taaacaacgc attacggggg aaaatatcac
       61 aaaaagaggt gtgttatggg aagggaaaga gaaagagaaa atcggagtta tacctacctt
      121 attgttggcc ttatagttgc aatacttaca tttgcatttc ttcttgcagc ttttggtatt
      181 gtgcttaatg gaaacataag tgtaagaaat ctgatttcat atatagttac agcaatatcc
      241 ttcggcgtta tttcaggcac cttccattat ttcaggctgt ttgttgcctt ctggttgttc
      301 attctaggca tattggtggg ttttgcagac atgttcagag tctttctcgt aaatgacttt
      361 ggctggaatg accttgttgg tcttatggcc tttttctcct atgtggtaat agggttcttc
      421 gccggtcttt tcttccaggc cattcactat ctatacaaac tgtacaaggc aaaaagacaa
      481 agacctcctg aaattaaata aaaatataga gctgtgcatt tacaacacac agctcgttaa
      541 atcatttact caactacctt gactccttca ggaaccttgt attcagtctt tatggtgcca
      601 tcctgattct tttcatgtct tacccagata atacccattg gtgtaggata ggttccttcc
      661 gcccattcca aatcaccaag gtcaggcttt attctaattt cctcgcatcc aggttttaat
      721 acttgtattc caagtacata acctgacatg aaagcagtag gacctgaagc ccatccatga
      781 cataaactat gcctgaatcc cttgtagcag aagtttccaa aatcaccatg caggtctttt
      841 actccttccg gaacaagttc gtcaattctt cccgaaccct caagccactc aatattgaag
      901 tcttcccaaa atgttgtagc gcctctgtca agcattcctc cccaataagt acgaatattg
      961 tcaagagcgc caaccatgtc atttgccaac gcctgtgcct gtaggatata gtagccaaag
     1021 aaagtggaca gatttctagc tcctccaacc ttgataactt tatcattcat ctcttctgca
     1081 ggcgctatgt ccgctagagc catcaaagct gcagcctgct tgttgtggtt tggatctgga
     1141 acatgtttca aaagtatgtc agctctttcc ctgcacatag ctgcggtctc gttttcacca
     1201 agctcttcaa gaagctcggc tcctcttaac aggctcaagc gcaatagcga atgaagtcct
     1261 gcatggaaag cctcatcatt tccatgggat ggccagtcaa gaaatcttgt tgggggcagt
     1321 tgttctctgc cttcttcatc aatatattgg ctgaactgaa tcaaaagact ctttaaataa
     1381 tccctctgtt cttcaagata ctctttatct ccattctgca tgtaccagcc atggtgaatc
     1441 agaacccacc acaaagagta tgaagatatg ccactcataa atccgggtag gggagtttca
     1501 tctcttgcaa ggtcaaggct ctttggtaca gattcatcat atccaaaaac agcctgaatt
     1561 gtggatgttt cgggatgcat atctccaatc cataccagtc tatctctctt gattccatcc
     1621 catacatatt cctgcatgtt gagatgaacg gtgtatgctc caacacccca gatttcattc
     1681 agtctttcat cgttacatct gaacgagcct ttgtactcca gttctctata agtaaaaata
     1741 ccttttactg tcttaaatga tattttggta ttaggatcaa tcaaatcaat tctgcaaaat
     1801 ctgaatccag aaggtccaat ttcaggcatg ctcaaaaatc cagtttcaat tatctgatcc
     1861 ctgttaatat ggtcgttggt ggcattcttt tctccaccta ggtcactcat agcctccatt
     1921 gcggattcac caaaacgaat acgaagcctt accttttcag gtgagacaga ccatacaaac
     1981 aactttacat atccatgaaa ctctacgcca aagtccagaa gtatgctcgc tgtttctcca
     2041 ttgttctcaa gcacacatgc ctctgtctga ccaagattaa tctgcggatc cctgtcttcc
     2101 agcaatactt tttcgcctgt tacaagacat ttgtctcctt ctgacttcca cagaaccttt
     2161 tgaacaccca taaagcgtct tactctcttg tcttcttccg tttttttaaa aatctccttc
     2221 ttacgtatac cataaggcac aatatccatc tgcataaaaa acctccaaat gcttgtttcc
     2281 gttagtatac taatggtttg gaataaagtc aatacggtgt aaagacacaa aaaaagaagg
     2341 aagctcttta ttcttccttc tttgtatgtt atgtcctttt atcatctcgg gcttatgcaa
     2401 aaaccctctt ctttaaaaca accactgcca taccaaaggc aaccgtcaaa gcaaataaag
     2461 caacaccgga atcagatgtc tgtggattgt cgtctttgtc tgtatatgtc ttaagagaaa
     2521 tattgtcaaa attagtcgga ctgtttctgt taccaatagc tactacgctg ttgacggcaa
     2581 acaatgcatt gtcatgagtg ccaagctcac tgccatcgcc accataaaca actaccttgg
     2641 aatagtattg ctcttcacca attggataag tcttcggatc tgagaacttg actcttgcaa
     2701 taatttcatc atctacagca acatatacgt ttttgccatc atcagccaat gtaatggtat
     2761 ggaatccggt tgaaaaatca taatttgtct caaaggcata aagcttgttg ttaatgtcgc
     2821 atttctgaga ctcatcataa agctttatgt acagagtaac ttttttgcct tcaaccctta
     2881 catacattcc acttcctcca agtgcttcaa ggccatcatg ctcaccggat ctgtcctgtt
     2941 caaagaagtc tgacatgtta agctcgtatt ggaacttctc tttaccggct cttgcaaaaa
     3001 taccaaagtt ttgtttccct gataccttga catcaataga gaacatgtag tacttttctt
     3061 ctactggttc atgaaacatc tgccagaagc catttgaagc aatatacttg tttccatccg
     3121 cttctgcaat ctttggctgt tcaccaagca caaactggta tgtccatcct gctggtggat
     3181 taccgaattc cgcacctacc gacatgcttt ccacatcaat tttagattct ttcacgagct
     3241 ctatcttcga catatcaagg tctgaaagct ttgcggcgtt tgcagccatt agtgaaaaaa
     3301 tcattacaaa gataacgcaa aacgcaaata acctcttgta catatttaaa acctccatta
     3361 acaattgtta agataattat attcatacaa cacattttgt caatatatta attatgtttc
     3421 ctgtttgttc acattggctt tactgcatac gaaagacaag gctttttgaa tataaagcag
     3481 aggactaata ttgtttgcgg tataaaatta tggtaaactt aagtcattat aaaaaattgg
     3541 aaggtgccgc cgatgcaaag aaaccgatca aattggacta ttaagaagat tatttttctt
     3601 ttcattatcc tgagtctgat aggaaaactc atatttgact tttcaggttt ttggctgaac
     3661 tttgcttttg tgtttcaata tataggatat attttaggct accttttatt tgggttcatt
     3721 atcgcatata tactcaacgc atatatagat ttctgggaca agaaactgtt caaggatttg
     3781 ctgaacaaca acaaaaaact taaaaaggca ttatgcatga ttattgcata catgaccttc
     3841 tttggaatac taagcttttt gatattcaca ctggtaccgg ctcttacaac cacacttaag
     3901 gatttcgcag ttaagattcc tactttaatt gacaaggttg tggcatatta caacaaccta
     3961 ttggacggta ccaaatatga attatccgaa gatgtcctga aaagcgtcac aaatgctctg
     4021 gattctcttc tcaagtcagc aacggatgct atcaacataa atctaattac ctccattgta
     4081 tcaaatgcaa caagcctgct ttttaatttt ataatgggca ttatggtttc agtttatatg
     4141 cttatagaga aggaacacac cttggttgcc ataaacaggg taattgatgg tctcttcaga
     4201 aaaagaaccg gagaacgctt gagatggata gggcatgagg tcaaccagat attcagaaag
     4261 tattttacag gaaaactgct tcaagcgctg atttgcacaa ttcttgcata tgttgcgttc
     4321 agtattgcaa ggctgcctta tgcaatactt tttgcagtca cattcggtat taccaacatg
     4381 attccgtata tcggtccctg gataggagca ataccgatta taattattag tcttgcgtat
     4441 gacttctggc ttggagttgc cgcagcaatc tgcatactta taatccaagc tgtggataac
     4501 tggtttatat cacccaagat tgttggcggc aaaatgggga ttagtccctt gctagtactt
     4561 gccggtcttg gtattggggg aaggctcttt ggcttgcccg gccttatatt cggcgacgtt
     4621 cttgcagcca tattcaagct gttcttctat gatgtttata tcagaaagag aattgaagcc
     4681 aaacaattga tatcgccggt taatgaaatc caagaagaaa aagaagatgc tgaaagcgaa
     4741 gttgttgagg aagaagttgt gactgaaacc gaggagacag tcagcataaa aacaaaaatg
     4801 gaaaccagat gggtctccat aaaagaaaag ttaagaaagg tatttaagag gaaatagcta
     4861 gttaatgaag cttttgtatc tcctcgcctc ataggcgaca atagtggagg caaaccccac
     4921 attgagggat tcaacacttc ccagcatcgg aatgatacag ggaattgcgc cttcgacatg
     4981 ccgccaatca ggagatattc ccagatattc attacctgca acaatacaaa ccctgccgct
     5041 gtaatctatg tcgtagtagg atttttttgc tgtcaaatca gtaacgatta tcttgaactt
     5101 gtttttgaca agccacttta caacctcggc catgtcgcaa acacatacag gcatcatgaa
     5161 agcagcacca agacttgctc ttataaccct gctgtgagtc agacgaactc ttctgttcgt
     5221 catgatggca aaatctccgc ctgctgcatc aagggatctt attgtagcac caacattgcc
     5281 tggttgttca agaccatcca gcaccataac aagcatgtcg tccttaagtg gaagtttttc
     5341 tatatcatac cttggcatgt ggcatattat gaagaagccg tccgcaccgt ctctatcact
     5401 tattttcctg caggcctttt gtgaaatctg gtatgaatcc ttgcagtact caatcatcag
     5461 atttgtcttt ttcttgtctt gttccgtctt tatcatgtca ggacagaaga aaaacatttc
     5521 cacctttatc ctctttttta taatcttctc aaccgcccaa agaccttcgc atatgaacaa
     5581 ctgttcattg tccaccttct tcccagtgga caaatccctt gcaagtttaa cttcttcgga
     5641 gttaatcccc tttatgggca catccataat ttaacctccc ttttcacaca aaaagggctc
     5701 ctccaagaag ccccctgtag taagtatctt tttgttatta atatttgtag attgtcttgc
     5761 ggcgctctaa ctcttctttc tctttcatct gctgagcctc ttgtgctata ttctttgccc
     5821 aggcaacagc cttgtcaaga atttcttgtg tttcttcatc cgtgtcacac caaataggct
     5881 caacacagtg gaacttgcca agaagaaccg agtcaacaag atagtccgcc agtttgtcaa
     5941 gagccttggt gtcttcttct tcaccagttg agaaaagcgc aatctttcta tcgtagatct
     6001 taattacatc aaaaagggta ttgtatactg ggttatagga atcgtaccag acaggagttc
     6061 ctataaatac aagatcgtag tctcttgggt ccttgcgcag agggaaaatg tcaggcttct
     6121 tctttctaac tacttccatt ctatgtttca actttgtaaa gaaaccgtat gcccttacac
     6181 ctttaactct tactatctta acaagatcgg catccaaagc gttagctatg gcttctgcca
     6241 ctgcctgtgt atttccgtcg acggtgttgt ataaaacaag tattttcatg aaaaaatgaa
     6301 ctcctttcat catagtcgta acgagtacaa caattatact ttacagcttt ttaaatcctt
     6361 tgtcaatgta atatttgtca tggcaacaaa gcttttataa acattttaaa aattatacat
     6421 tgatggcaac tttcttgact ccctaacaat attatcacca tacacaatta ttggttttat
     6481 gacaaaagag tacacaaact ccttctcatt aaactcgtat ttccggttca gttccggacc
     6541 acagctgtgg gatcctattc cattttgttt atagtcaatg tgtattactg tttcctccaa
     6601 cggaacaagg tccttgtcat ggggagtatt atccaacatc ttcggagtgt agtgaagact
     6661 gctgaaatta aacttgggca ttcccttgaa tagcagccct cttccttcca aatcataaac
     6721 tgccgcaaat ctgcaatcga tatggttacc tgtttcctgc gggaagatgt attttgtata
     6781 ctcatcagtt acagtcgagt taaacaatcc cagtttggag gcattcttca tatctgcata
     6841 ggaagagaca ggacctctac caaagtacgt taccttctca aaacccttgg gcattacaag
     6901 catcatgcca aacctcggca acgattttat attcttcctc acctctgcag caacagagac
     6961 tccaatctca ccacttccat aaaccgtcca cataacggaa tatttgacta caggcagtct
     7021 tgacggacct gccagtgaat atgatgctgt tatgctgata aaagtaggat taatggatga
     7081 aattttcaaa tcatacagtt tttcctttgc attgtgcatc tcctcccact gccatgcatt
     7141 ccttacatgt ctatcattat cggtaggagc cctccatata ttgaaagcag tagggcagga
     7201 cagaagttca acaccatcca actccaggct gtcgaactgc cctttttcca catcaaatct
     7261 gtaaacgaag ttgtgtcctt caatttctac aagacctttg ccagtttccc taaactcaac
     7321 ttttggcata ctgcttgccc atatcatttc aggctccagt tgtttaacag g
//
LOCUS       DAONVY010000037         7234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7806_length_7234_cov_3.106143, whole genome shotgun sequence.
ACCESSION   DAONVY010000037 DAONVY010000000
VERSION     DAONVY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7806_length_7234_cov_3.106143"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..228
                     /locus_tag="PLI11_01765"
     tRNA            153..228
                     /locus_tag="PLI11_01765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:186..188,aa:Lys,seq:ttt)
     gene            340..1671
                     /gene="hisS"
                     /locus_tag="PLI11_01770"
     CDS             340..1671
                     /gene="hisS"
                     /locus_tag="PLI11_01770"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPZ51621.1"
                     /translation="MNQNKDTIVKPSILSGFLELLPEDQIIFNNMLDTIRSTFESYGF
                     IPIDTPVIEKAEMLLAKSGGETEKQVYRFTKGDNDLALRFDLTVPLARFVAQHYGVLT
                     FPFRRYHIGKVYRGERPQKGRFREFYQCDIDIVGDEKLSVINDAEIVSVIHSTFKKLG
                     LDEFTIMINNRKLLNGFFMHYGIKDNVEVLRIIDKLDKIGADNVRKELVASGISENVA
                     DEILEFTNLCGDPDDVISALRQIDIDNDDFVSGLEELETVWSYIKAFGLDSSEIEINL
                     KIARGLDYYTGTVYETILNHHKELGSVCSGGRYDNLASNYTKKKLPGVGISIGLSRLF
                     YQLREANVISVSGVKTTTKVLFVPLGDTSAYAIKTANYIRRKGVNTEVYLNDGKLAKK
                     LNYANKLGIPYVAIIGEDEVKKNTVCIKDMNSGEQSVYTIDELTEFVKNIM"
     gene            1690..2142
                     /locus_tag="PLI11_01775"
     CDS             1690..2142
                     /locus_tag="PLI11_01775"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF014694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HPZ51622.1"
                     /translation="MVKSFQVDHTNMKAPDIRIAEEIPVGRQNKIIKYDIRFKRPYKD
                     DPMYPATMHSIEHMMATSIREISQATIIDFSPMGCRTGFYLTVLNPPENFISNDIKGA
                     MIISLDMDEVPGADKKSCGNCYYHDIDDAKRELLDFLEFYYGIRLILR"
     gene            2208..2699
                     /locus_tag="PLI11_01780"
     CDS             2208..2699
                     /locus_tag="PLI11_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51623.1"
                     /translation="MKHRNLLTNIFSVVLIVSLIAFLFVTIRGMEGGLGDTIRYISLP
                     LAIISVACIDIVFPILDNKQRLRENNNLKILTIVKIVLFIASFVVIGLYVTNIILNDD
                     TEMVAVAVFVVLYFAQFFINIDPKQPRVSEVSDDEEYDVEEYDEDDDTDAEYEETGSE
                     QDE"
     gene            complement(2778..5390)
                     /locus_tag="PLI11_01785"
     CDS             complement(2778..5390)
                     /locus_tag="PLI11_01785"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPZ51624.1"
                     /translation="MKSKDNFNKPYGLLINLSPCPLGVDLNPKFSWILDSSKKNDHQT
                     AYRIIISDNKQKINSNHGNIWDSGIVASSESSNIFYAGDELLPDKTYYWKVCTFDKDS
                     EQSEFSEPQKFTTLIKEWKAKPIWCDNNADMVFIRKEFEISKEVESAIASVFASSTET
                     ARQYVFDFRVNGKFISQGPVKPHKNKHFYNTYDITDELTKGMNCIGSLLFTRKDKVYM
                     CQLTITYTDGTVERIGTDKTWLAMDAKDVFGMTRSIGTVLYFYPSEDINANLYPFGFD
                     KAGFHNESFTHAIEKEFDHQLYPVYTTKVDRFYKHAQSVVKLGENEYFIDIGKEVIGG
                     LKLCIEVPKAYANQTIRIIFGEELEEENQVRYKMRTTNTYEEFWTLKEGYQEIENFGM
                     KAFRYVNIQNCPMQLTTKNIMGVAYRQHYDANDSFFESSNSLLNDIYDFVKYSITVTS
                     QDLYVDSQTRERKNYEGDAYINQLSQYALMRNFTLPRFSIEDLVYCPTWPFEYKQMTI
                     MMALNDYLHTGDKGLLEAHYDMLKTKVVDGNNDPDNYFDETLGLVFNTTKNNSENANL
                     VDWPMSERDNYDFSNAYYNTVTNAFHYKAYGDLAQIAEVLGIYEDASKYSKYAERIKE
                     SMYKHLIDEDIGLFVDGLDKDGIRLTHMSQHANIFPLALGIVKDYETIERIVKFIDER
                     GMCTSVYGAHFVLAACYQANAGQTALNLMISRKLRSWYHLIYELNATVCPEAWDPANK
                     PNMTYSHPWSSAPANALAYGMFGIKPTKPGFKTFDIKLQPGNLEYAKIKVPTIKGHIE
                     VEYNLNIFTDSETEVCVNAKIPVNTTAKVFIPCKKENPEVLLNGIETGFEYENGYVAV
                     DNVGSGKHYIVVKS"
     gene            5498..6466
                     /locus_tag="PLI11_01790"
     CDS             5498..6466
                     /locus_tag="PLI11_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPZ51625.1"
                     /translation="MNYSAERINNYIRENRFGELIEISEQRHRKQFEELREMIRNANK
                     DVQHEKVTEAVLITGPSSSGKTTFAHLVAEMLSEDGYDCTVISFDNYYKDRAEIQKMH
                     LESGFVPTEENDYDYETLDAFDVEFFKKQMNEYLAGNEIVLPKYDFTTGTRVPGDIVL
                     KPTEKDMLIIEGIHALNPALRTGVDFMFSFNVYICPFDFFGADQNEQVIRPQELRFMR
                     RAVRDSVKRGATLRRTMEMWPNVRRGEEKFIKPMKAYTDFFFNSSLEYEIAYLKKKIF
                     EMRDELDEEGKQMLEKFVPVRTLEYFDGIDGFEIPDKSIFNEFYIK"
     gene            6553..>7234
                     /locus_tag="PLI11_01795"
     CDS             6553..>7234
                     /locus_tag="PLI11_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPZ51626.1"
                     /translation="MKTKAVRLYGKKDLRLEEFELPALKDDEILARVISDSICMSTYK
                     AAIQGADHKRVPDNVAENPIIVGHEFCGEILEVGAKWQSKFKVGQKFTIQPALNYKGS
                     LDAPGYSYPYIGGSATYIVIPNEVMETDNLLVYNGEGFFAGSAAEPMSCVVGTFHAMY
                     HTTSGSYEHKMGVVEGGNMAILAGVGPMGLCAIDYAIHRDRRPGLLVVTDINQERLDR
                     AASIYTVEE"
BASE COUNT         2134 a         1258 c         1581 g         2261 t
ORIGIN      
        1 agaaagaagc ttttgacagt atggagcttg tcgttgtaga gtagaatata tggtgatgca
       61 aggcttgtcg cacttttcat gcattttttt agaaaaaggt gttgacaagc cggttgaaat
      121 gtagtattat aatcaggcgc caaaaaataa aagatccatt agctcagtcg gtagagcacc
      181 tggcttttaa tcagggtgtc cggagttcga gtctccgatg gatcaccaac cgtgcaactc
      241 caacgaaaca catgtttcct tggagttttt atatgcgtaa tgattgacaa gatctcttct
      301 ttcaattata ctttacgcga acatatttgg gaggatctta tgaatcaaaa taaagataca
      361 atagttaaac catctatttt atccggtttt cttgagttgc ttccagaaga ccagataata
      421 tttaacaata tgctcgatac catcaggagt actttcgaga gctacggttt tatacccata
      481 gacactccgg taattgaaaa agccgagatg ctgcttgcaa aaagcggtgg agaaactgag
      541 aaacaggtat acagattcac caagggagat aatgatctgg ctcttagatt cgatctgacg
      601 gttcctcttg caaggtttgt tgcacagcat tatggagttc ttacgttccc ctttagaaga
      661 tatcatatag gcaaggtata tcgtggagaa cgtcctcaaa aaggccgttt cagagagttt
      721 tatcaatgtg atattgacat tgtcggagac gaaaaactca gtgttattaa tgatgctgaa
      781 attgtaagtg ttattcattc tacattcaag aagctcggtt tggacgaatt tacaatcatg
      841 ataaacaacc gcaagctatt gaatggattt ttcatgcatt acggtattaa ggacaatgtg
      901 gaagtattaa gaatcataga caaactggac aagataggtg ctgacaatgt caggaaggaa
      961 cttgttgcga gtggtatatc tgaaaatgtt gccgatgaaa tacttgaatt tacaaatcta
     1021 tgtggcgatc cggatgatgt gatatcagct cttagacaga ttgatataga taatgatgat
     1081 tttgtttccg gtttggaaga gttagaaacc gtatggtcct acataaaggc gttcggtttg
     1141 gattcgtcag agattgaaat caatttgaag atagcaagag gtctcgacta ctataccggc
     1201 actgtttatg agaccatact caaccatcac aaagaacttg gaagtgtgtg ctctggtgga
     1261 agatacgaca accttgcatc aaattataca aaaaagaaac taccaggtgt aggaatatca
     1321 ataggattgt caagattgtt ttatcaactt agagaagcca atgttattag cgttagtggc
     1381 gtgaaaacaa ctaccaaggt tctgtttgtt ccgctaggag atactagtgc atatgccatt
     1441 aagacagcta actatatcag gcggaagggt gttaatacag aagtttattt aaatgacggt
     1501 aaattagcaa agaaattgaa ttatgcaaac aagctgggaa tcccttatgt tgcgattata
     1561 ggggaggatg aagtcaagaa aaatacggtt tgcataaagg atatgaatag cggagaacaa
     1621 tcagtttata caattgatga attaacggag tttgtaaaga atataatgta acaggagagg
     1681 aagaaggata tggtaaagtc ttttcaagtt gatcatacca acatgaaagc acctgatatc
     1741 aggattgcag aagaaatccc cgtaggaaga cagaataaaa taatcaaata cgatattcgt
     1801 ttcaaaagac catacaagga tgaccccatg tatcctgcaa ccatgcacag tatagagcat
     1861 atgatggcga catcgataag agaaataagt caggctacaa ttattgattt ttctcctatg
     1921 ggatgccgta ctggttttta ccttacagtg cttaatccgc cggaaaactt tatttccaat
     1981 gacatcaaag gcgcaatgat tatttccttg gacatggatg aggtcccagg tgcggataaa
     2041 aagtcctgtg gcaactgcta ttaccatgac attgatgatg caaagcgcga gctgcttgat
     2101 ttccttgagt tttactatgg aatcaggctt attttaaggt aatgattagt tgacatattc
     2161 aacgtataaa tatacaatgt aggtataatt ttcaaggggg taataaaatg aaacatagga
     2221 atcttctgac aaatatattt tcagtggtgt tgattgtttc gcttatagca tttctattcg
     2281 taacgattag aggcatggaa ggtgggttgg gtgacactat aagatacata agcctgccgc
     2341 ttgcaatcat ttccgttgca tgcattgata ttgtctttcc aattttggat aacaaacaaa
     2401 gattgcgcga gaataataat ttgaaaatat taaccattgt gaaaatagtt ttgtttatag
     2461 cttcctttgt agttatcggc ttgtatgtta caaatattat tctcaatgac gatactgaga
     2521 tggttgctgt cgcagtgttc gttgtcttat actttgcgca gttctttatc aatatcgatc
     2581 ccaagcagcc aagagtgtct gaagtttcag atgatgaaga atatgatgtc gaagagtatg
     2641 atgaggatga cgatacagac gctgagtacg aggaaacggg ttcagaacag gatgaataag
     2701 tgaaaataaa agatgccccc ttttaggtga catctttttt ttggtcttat gtcggtatta
     2761 ggctaagacc tttgttttta gcttttgact acaatgtaat gttttcctga acctacattg
     2821 tcaacagcaa cataaccatt ttcatattca aagccggttt caattccgtt cagcaggact
     2881 tcgggatttt ccttcttgca gggtataaaa accttggctg ttgtatttac cggtatttta
     2941 gcattgacac aaacttcagt ttcgctatcg gtgaaaatat taaggttgta ctcaacttca
     3001 atatgtccct taattgtagg gacttttatt tttgcatact ccagattgcc cggttgcagc
     3061 ttaatatcaa atgttttgaa tccaggtttg gttggtttga ttccaaacat accatatgca
     3121 agagcatttg caggagcaga gctccagggg tgggagtatg tcatgtttgg tttgtttgcc
     3181 ggatcccaag cttcaggaca tactgtagca ttcagctcat atatcagatg ataccagctt
     3241 cttaactttc tgcttatcat aagattcaat gcagtttgac ctgcatttgc ctgatagcat
     3301 gccgcaagga cgaaatgtgc accgtaaacg gatgtgcaca tgcctctttc atcaatgaat
     3361 ttaactattc tttctattgt ttcatagtcc ttgacaatac ccagtgcaag agggaagata
     3421 tttgcgtgct gggacatgtg ggtaagtctg atgccgtcct tgtcaagtcc gtcgacaaac
     3481 agaccgatat cctcatctat aaggtgtttg tacatgcttt cttttattct ctctgcatat
     3541 ttgctatact tcgatgcatc ttcataaatg cccagaactt cagcaatctg cgccaggtct
     3601 ccatatgctt tgtagtgaaa agcatttgtc actgtgttgt aatatgcatt ggagaagtcg
     3661 taattgtctc tttctgacat tggccagtca accaggtttg cgttttcgct gttgttctta
     3721 gtagtgttga agacaagccc taaagtttcg tcgaaataat tgtcagggtc attgtttcca
     3781 tccaccactt ttgttttaag catatcgtaa tgcgcttcaa gtagtccttt gtcgcctgtg
     3841 tgcaggtagt cgtttaaggc catcattatg gtcatttgct tgtattcaaa tggccatgtc
     3901 gggcagtata caagatcttc gatggagaaa cggggaaggg tgaaattgcg cattaatgca
     3961 tactgggaaa gctggtttat gtatgcatcc ccttcgtagt tttttctttc ccttgtttga
     4021 ctgtctacat acaaatcctg gcttgttact gtgattgaat atttaacaaa atcatatatg
     4081 tcgtttaaaa ggctgttcga gctttcaaag aaactgtcat ttgcgtcata atgctggcgg
     4141 taagccacac ccattatgtt ttttgttgtt agctgcatgg gacaattctg gatgttcaca
     4201 tatctgaagg ctttcattcc aaagttttcg atttcctgat atccttcctt cagcgtccag
     4261 aattcctcat atgtatttgt tgtcctcatc ttatagcgta cctggttttc ttcttccagt
     4321 tcttcgccaa aaattattct tatggtttga ttcgcatatg cttttggaac ttcaatacaa
     4381 agcttcaaac ctccaatcac ttcttttcca atgtctatga agtattcatt ttcaccgagt
     4441 ttgacaacgg attgggcatg tttatagaat ctgtctactt ttgttgtata tacagggtaa
     4501 agctggtggt cgaactcttt ttcaattgca tgtgtaaagg actcgttatg aaaccctgcc
     4561 ttgtcaaaac cgaaaggata aagatttgca ttaatatctt ccgaagggta gaagtacaga
     4621 actgtgccta tgcttcttgt cattccaaat acatctttgg catccattgc aagccatgtt
     4681 ttatcagtac cgattctttc gacagtgcca tccgtatatg tgatggtcag ttgacacatg
     4741 tatactttgt cttttctggt aaacaggagg ctgccgatgc agttcatacc ttttgttagt
     4801 tcatcggtta tgtcatatgt attgtagaag tgtttgtttt tgtggggttt cacgggtccc
     4861 tgacttatga attttccgtt taccctaaag tcaaatacat actgccttgc cgtttcagtg
     4921 gaggaggcaa aaacagaagc gattgcagat tccacttctt tggaaatttc aaattccttt
     4981 cttatgaaaa ccatgtctgc attattatca caccatattg gttttgcctt ccattcttta
     5041 attaatgttg tgaatttttg aggctcggag aattcgcttt gttcgctgtc tttgtcaaaa
     5101 gtgcagactt tccagtagta agttttgtca ggcaaaagtt catctcctgc ataaaatata
     5161 tttgaggatt cgcttgaggc aacaataccc gaatcccata tgttgccatg attgctattt
     5221 attttttgct tattatctga tataattatc ctgtaggccg tttgatgatc gttctttttg
     5281 gaactgtcaa gaatccatga aaactttggg ttcaaatcga ctcccaatgg gcatggactt
     5341 agattaatca gcaaaccgta gggtttgtta aaattatctt ttgatttcat tgcctgtatc
     5401 ctttcttgta ttgttaatgg atgaatttta gcatactatt gttagtattt caacaatatg
     5461 ctatactatt tggaaaatta agacggagga gtgtttgatg aattactcag ctgaaagaat
     5521 aaacaattat atacgtgaaa atagatttgg tgaactaatt gaaatatccg aacaaaggca
     5581 caggaagcag tttgaagaac ttcgagagat gataagaaat gccaacaagg atgtgcagca
     5641 tgaaaaggta accgaagctg tattgataac gggaccgtca tcctcgggaa agactacatt
     5701 tgcccatttg gtggcagaaa tgcttagtga ggatggttat gactgtacgg taatatcatt
     5761 cgataattac tacaaggata gagctgaaat acagaaaatg catcttgaaa gcgggtttgt
     5821 tcctaccgaa gagaacgact atgattatga aactctggat gcctttgacg ttgagttttt
     5881 caagaagcag atgaacgagt atctggctgg caatgagata gtgctgccaa agtatgattt
     5941 tacaacaggt acaagagtgc ccggtgatat tgttctgaag cccaccgaaa aggacatgtt
     6001 gattattgaa ggcatacatg cgctgaatcc tgccttgagg actggtgtgg atttcatgtt
     6061 cagtttcaac gtgtacattt gtccattcga cttctttggt gctgaccaga acgagcaggt
     6121 tatcaggcct caggaactga ggttcatgag aagggctgta agagacagtg tgaaaagggg
     6181 agccaccttg agaagaacca tggaaatgtg gccgaatgta aggaggggtg aagagaagtt
     6241 tatcaagccg atgaaggcat ataccgactt ctttttcaat tcgtctctcg agtatgagat
     6301 cgcgtatctg aagaagaaaa tattcgagat gagggatgaa ctggacgaag aaggaaagca
     6361 aatgctggag aagtttgtgc ctgtcaggac ccttgaatat tttgatggga tagacggctt
     6421 tgaaatcccc gataaatcaa tattcaacga gttctatatc aagtaaactc ggttgacata
     6481 acatgcaatt taatgtaaca ttatcatagg cttaatagtt attaagttag tataattact
     6541 aggaggaatg tcatgaagac taaagcagta aggttatacg gtaagaagga cttgagactt
     6601 gaggagttcg aattaccagc attaaaagac gacgaaatat tggcccgtgt tatatctgac
     6661 agtatttgta tgtcaacata caaggcagct atacaagggg cagatcataa acgtgtgccc
     6721 gacaatgttg ctgaaaatcc aattattgtt ggacatgaat tttgcggaga aattttagag
     6781 gtaggagcaa aatggcagag caagttcaag gttggtcaga aatttacaat acagcctgct
     6841 ttgaactaca aaggctcatt ggatgctcca ggatactcat atccatatat aggcggttct
     6901 gcaacctata tcgtaatccc aaacgaggtt atggaaaccg ataatctgtt ggtatacaac
     6961 ggagaaggat tctttgcagg aagtgcggct gagccaatgt cctgtgttgt tggtacattc
     7021 catgccatgt atcataccac cagtggaagc tatgagcata agatgggtgt tgtcgagggt
     7081 ggaaacatgg ctatccttgc aggtgtggga ccgatgggtc tttgtgccat agactatgca
     7141 atacatcgtg acagaaggcc aggacttctt gtcgttaccg atataaatca agaacgtctc
     7201 gatagagctg cttcaattta cactgttgaa gaag
//
LOCUS       DAONVY010000038         7197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7868_length_7197_cov_3.492019, whole genome shotgun sequence.
ACCESSION   DAONVY010000038 DAONVY010000000
VERSION     DAONVY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7868_length_7197_cov_3.492019"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..981
                     /gene="plsX"
                     /locus_tag="PLI11_01800"
     CDS             127..981
                     /gene="plsX"
                     /locus_tag="PLI11_01800"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPZ51627.1"
                     /translation="MDKDYDKERLTIKHASEVITNNEVPTKAIKKKTDSSMVVGFNML
                     KNREGDVFVSAGSSGALLAGSVFILRRIRGISRPALGSIIPTKRGRIILLDSGLNSVI
                     RPQNYLQFAYLGAAYMKSMFGVEDPRVGLVNVGTEEEKGTEAVKEANTLLRQSTLNYI
                     GFIEGKDVFEGIADVVVTDGFTGNVMLKLIEGTATYFFREVKGVLLRNWKSRIGAAFL
                     KKGFEDFKKSMDPDVNGGAPILGVDGLVIKSHGSSNARTIKHVILKAVTLAESDFISD
                     IKKEIKNM"
     gene            1045..1275
                     /locus_tag="PLI11_01805"
     CDS             1045..1275
                     /locus_tag="PLI11_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPZ51628.1"
                     /translation="MKMFDKIKKIVVEQLGVDESEVTPEASFIDDLGADSLDIVELIM
                     AMEDEFGIEIPDDVAEKITTVGDAVEYIKTIQ"
     gene            1356..2036
                     /gene="rnc"
                     /locus_tag="PLI11_01810"
     CDS             1356..2036
                     /gene="rnc"
                     /locus_tag="PLI11_01810"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPZ51629.1"
                     /translation="MNKKKLNDIEKILGYSFTDKSLLTTALTHSSYANEQTPKVPHNQ
                     RLEFLGDAVLELIISEHIYKKNPGMHEGELTKLRAALVCEDTLYKVASDLCINEYILL
                     GAGESQREVRMSIMADCFESIVAAIFLDGGYKAAKNFINRNLEKIFKYGSASGQKINY
                     KTELQEKYARTHSIVYTITDVEGPEHDCVYTARAEVGDKLVAYGTGRSKKEAEQDAAR
                     RILEMDLG"
     gene            2098..2817
                     /locus_tag="PLI11_01815"
     CDS             2098..2817
                     /locus_tag="PLI11_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPZ51630.1"
                     /translation="MIKVTVWNEFIHENVNEHVRSIYPEGIHNEIAKFLSKNEDIQVR
                     TATLREPNHGLDDEILDDTDVLIWWGHAAHHEVSDEIVAKVHECVLKGMGFIALHSAH
                     YSKPFIKLMGTSCSLRWRDDDRERLWCVNPGHPIARGVPEYFEIPNEEMYGEFFDIPT
                     PDELVFLGWFRGGEVFRSGCVFNRGYGKVFYFQPGHETHPIYKDENIQRIITNAVYYL
                     NPVKRRERISADCTKAPEEDL"
     gene            2787..3803
                     /locus_tag="PLI11_01820"
     CDS             2787..3803
                     /locus_tag="PLI11_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPZ51631.1"
                     /translation="MHKSTRRRLVIPIFVPHVGCPRLCVFCNQRSISGNINIPSRQET
                     TDTIEQYLEVANRYDVVEVAFFGGSFTAIHPALQEDFLSCVYPYIGKGVDYVRISTRP
                     DCINDEILMRLKKYRVHVIELGAQSMDDKVLEASLRGHTSRHTIEASELIKREGFVLG
                     LQTMPGLPMSDRNSDIETAKKIAALGPDLVRIYPTIIIKKTHLETMYRQGKYEPQTLE
                     YMIDLCAELVEIYEKEGIDIARIGLQSSENMTEEKEIVAGPYHPAFGQLVHSRRALQG
                     IVDRVREMRIGGGKVLIRVPEKELSTYIGQRRSNVLKLKEMFNFEDVRFIPDPLMDNG
                     FVLE"
     gene            3834..5105
                     /locus_tag="PLI11_01825"
     CDS             3834..5105
                     /locus_tag="PLI11_01825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPZ51632.1"
                     /translation="MDKDRISTSYIGVLDGVRAFSVFIVAFFHFWQQSWYNKVIYYSW
                     FDKIGMKEINLEWLWRTGYVFVDMLILLSGFLLFLPHARHMVEGRKLDSTVTFYRKRI
                     ARIVPSYYLSVLFLLFFSVIPKQQFSSIEHLKKDLFAQLTFTQMFDPMTKNTMFNGVL
                     WTVAVEVSFYIIFPLVAYFFKKKPVITYICMTLTSFVYIQQRVLKAKDLGFMINQMPT
                     FLGVFANGMMGALIFVHIANRYKREKYLSAMFTLVSILSLYMIRLILKESLLRAQSNQ
                     VWQVEYRYILSLVFLMFIISLSLSAKWLRFIFSNRFVRYLATISYNFYIWHQIIAVKF
                     KFGMGKNNQIRIPHWEGDRSPNFTGDKAWQIKYTLIILFASIIVATILTYFFERPMAN
                     LILGKKKSKKVKEQLIEEYVVDRENLTDNAI"
     gene            5140..5763
                     /locus_tag="PLI11_01830"
     CDS             5140..5763
                     /locus_tag="PLI11_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPZ51633.1"
                     /translation="MRIRMFGRVVYMPPAFVATVVLIIILALAIIGYNLSENDRDAIT
                     NHTPTPPLTQSEIQVTDPPLISVHVIGCVKEKKVVKIPVGSTVEDAVVAAGGFTEEAD
                     TDAVNLAYRLSDGMQIRIPSIDDEDKTWIVSHGDNGGNGGNNEKISINKASVAELATL
                     PGIGQTLALRIVDYREKNGGFKTLEEIMNVPGIKEAKFESIKDYITL"
     gene            complement(5876..6646)
                     /locus_tag="PLI11_01835"
     CDS             complement(5876..6646)
                     /locus_tag="PLI11_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HPZ51634.1"
                     /translation="MKIEKVSEDVIRITLTAEDLKKRNLDAQKFKYDTPQYERLLLDV
                     ISHAEIEFGFDISECRVVHEPASNKQEDHIITVTRTSNAKNIDNSTFDFNPAYLLKIL
                     AYLKELLPIDENFDFDGEDKDFSDELHHNDYFLNSILLYDENEKAKEKRYSDESCNEN
                     YEVLVFNDFENLISMVLTIPQSRNVPASLYKYNDNYYISYRLSKRNAGVIEKIRDIAS
                     EFDGKYMPASITRPILEEHANKIIKRGAFSVLLNNFAK"
     gene            6905..7174
                     /locus_tag="PLI11_01840"
     CDS             6905..7174
                     /locus_tag="PLI11_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HPZ51635.1"
                     /translation="MRRNKREALVLIAFMFIGSIIGGIVGKLLSYIPHLEFIDKIGKS
                     NILSVSFNPLFDINVIRFGFDLSLGVNIGSIVGIILAIAIWSRRY"
BASE COUNT         2288 a         1238 c         1631 g         2040 t
ORIGIN      
        1 cggactctgg gcggcgtttg ttatctttac atttccttta atcttttagc atctaataga
       61 atttttagga ttctgcaacg gtttaagaga atacttcaat aaacatgtcg ttgtgacctt
      121 atttttattg acaaggatta tgacaaggaa agactaacca taaagcatgc ttcagaagta
      181 ataaccaaca acgaagtgcc caccaaggcc atcaagaaga aaacggattc ttcaatggtt
      241 gtcggtttca atatgcttaa gaacagggaa ggagacgttt tcgtatctgc tggaagcagt
      301 ggagcattgc ttgcaggaag cgtattcatt ttaagaagaa taagaggtat ttcaaggcct
      361 gctcttggct cgataatacc tactaaaagg ggcagaatca tactccttga ttcagggttg
      421 aactctgtca ttaggccgca gaactatctg cagtttgcat accttggtgc agcctatatg
      481 aagagtatgt tcggtgtcga ggatcccaga gtagggcttg taaacgtggg aaccgaggag
      541 gaaaaaggaa ccgaggcggt aaaggaggca aatacacttc ttagacagtc taccttgaac
      601 tatatcggtt ttatcgaagg caaagatgtg tttgagggga ttgcagacgt tgttgtaacg
      661 gacggattta caggaaatgt catgctcaaa cttatagaag gtactgcaac atactttttc
      721 agggaagtaa aaggtgtatt gttgcgcaac tggaaatcaa gaatcggagc agcgttttta
      781 aagaagggtt ttgaggactt caaaaaatcc atggatccgg atgttaatgg aggagctcca
      841 atacttggag tcgatggcct ggtcataaaa agccatggaa gctccaatgc tcgcacgata
      901 aagcacgtaa tattgaaagc agtaacgctg gctgaaagtg actttattag cgacataaag
      961 aaggaaataa aaaacatgta atattatggt gtatataact aaattaaaat agacaataat
     1021 aataaacaaa atttgaaagg agaaatgaaa atgtttgata aaatcaagaa gattgttgtt
     1081 gaacagcttg gtgtagacga aagtgaggtt acaccagagg cgtcattcat cgatgacttg
     1141 ggagcggatt ctcttgatat agtggaactc atcatggcta tggaagatga attcggtatc
     1201 gagattcccg acgatgtggc ggaaaaaatc acaaccgtag gagatgcggt agagtacatc
     1261 aagacaatac agtaataata aatactgtta tatatcataa ggtgtaattt gcgtaattac
     1321 accttgtgat atttttatac caaaggatga gtaggatgaa caagaaaaag ttaaacgata
     1381 ttgaaaaaat attagggtat tcgtttacag ataaatcatt gctgacaact gctttaaccc
     1441 acagctccta tgcaaatgaa cagaccccga aagtacccca caaccaaagg cttgagttct
     1501 tgggagatgc ggttctggaa ctcataataa gcgagcatat ttataaaaag aatccaggca
     1561 tgcatgaagg ggagcttaca aagctaagag ctgcgctcgt ttgcgaggac acattataca
     1621 aggttgccag tgatttatgt ataaacgaat acattctatt gggagcagga gagtctcaaa
     1681 gagaggttag aatgtcaatt atggccgact gttttgaaag cattgtggct gccatttttc
     1741 ttgatggtgg ctataaggct gccaagaact tcataaaccg caatctcgaa aaaatattta
     1801 agtacggcag cgcaagcggc cagaagataa actacaagac ggaacttcag gagaagtatg
     1861 caaggacaca cagcatagta tatacaatta cggatgtgga aggtcctgaa cacgattgcg
     1921 tttatacagc aagagctgaa gttggagaca agttggtggc atacgggaca ggcagatcaa
     1981 aaaaggaagc agaacaagat gctgcaagaa ggattcttga aatggacctt ggataagctt
     2041 tataattgtc ctcttatgtg ttagaataat tttaaaaaga tgataggaga aggttttatg
     2101 ataaaggtta ctgtttggaa tgaatttata catgaaaatg taaacgagca tgtaaggagt
     2161 atttatcctg aaggcattca taacgaaata gcaaagtttt taagcaaaaa cgaagacata
     2221 caggtaagaa ccgctacatt gagagagcct aatcatgggc ttgacgacga gattcttgac
     2281 gacactgacg tccttatttg gtggggacac gcggcccatc acgaggtatc tgatgaaata
     2341 gtggcaaaag tacacgaatg cgttctgaaa ggcatgggct tcatagcgct tcattcagcc
     2401 cactactcaa aacctttcat aaagcttatg ggcaccagtt gttctctgag atggcgtgat
     2461 gacgacaggg aaaggctttg gtgtgtaaat ccaggacatc caatagccag aggagttcct
     2521 gagtattttg aaataccgaa tgaggaaatg tatggagaat tctttgatat accgacaccg
     2581 gatgaactgg ttttcttagg ctggtttaga ggcggagaag tattcagatc aggttgcgta
     2641 ttcaacagag gatacggaaa agtattctac ttccagccag gtcatgaaac tcatccaatt
     2701 tacaaggatg aaaacataca gaggatcatt acaaacgcag tatactatct aaatccggtt
     2761 aaaagaagag aaaggatatc agctgattgc acaaaagcac cagaagaaga cttgtaatac
     2821 caatattcgt acctcatgta ggatgcccca gactttgcgt attctgcaac cagaggtcaa
     2881 ttagtggaaa tattaacata ccttcaaggc aggaaacgac cgacaccata gagcagtatc
     2941 tggaagttgc aaacagatat gatgtggtgg aggttgcttt ctttggaggc agttttaccg
     3001 ccatacatcc tgccttgcag gaagatttct tatcctgtgt gtatccatac attggcaagg
     3061 gtgttgatta cgtaaggata tcaacaaggc cggattgcat aaatgatgaa atattgatga
     3121 gattgaaaaa gtacagggtt catgtaattg aacttggtgc tcaatccatg gatgacaagg
     3181 tactggaagc gtcattgcgc ggacacacgt caaggcatac cattgaagcc tcggaattga
     3241 taaaaagaga aggtttcgtt cttggccttc agacaatgcc gggtctgcct atgtcggata
     3301 gaaatagcga cattgaaaca gcaaagaaaa ttgcagcact tgggcctgat ttggtcagaa
     3361 tctatcccac aataatcatt aaaaaaacac accttgaaac aatgtacaga caaggaaaat
     3421 atgagccgca gacccttgaa tacatgattg acttatgtgc cgaacttgtg gaaatctatg
     3481 aaaaagaggg aattgatatt gccaggatag gtctacagtc atctgaaaac atgactgaag
     3541 aaaaggaaat agtggcagga ccatatcacc ctgcgtttgg ccagttggtg cattcaagaa
     3601 gggctttgca aggaatagtg gatagagtga gggaaatgag gattgggggc ggtaaggttt
     3661 taatcagagt tcctgaaaag gaattgtcga cgtatatagg gcagagaaga agtaatgtat
     3721 taaagcttaa ggaaatgttc aattttgagg atgtaaggtt tattccggat ccgctcatgg
     3781 ataacggatt tgttttagag taatcgtcat catgggaaag gtagtaggtt aaagtggaca
     3841 aggataggat aagtacatca tatataggtg ttctggatgg agttagggca ttctccgttt
     3901 tcattgtggc cttctttcac ttttggcagc agtcctggta caacaaggtc atatattatt
     3961 cctggtttga caagattgga atgaaggaaa tcaacctgga atggttgtgg agaacggggt
     4021 atgtgtttgt cgacatgctg atacttctaa gcggctttct cctatttctg ccgcatgcaa
     4081 ggcacatggt ggaaggaaga aagcttgaca gtactgtaac cttttataga aaaaggattg
     4141 cacgaatagt tccctcatat tatctgagtg ttctttttct gttgtttttc tcagtaattc
     4201 ccaaacagca gttttcatct atagagcacc tgaaaaagga cttgttcgcc cagcttacat
     4261 ttacacaaat gtttgatccc atgacaaaaa acaccatgtt caacggtgtt ttatggaccg
     4321 tggcggtgga agtctcgttt tacataatat tccctctcgt tgcctacttc tttaaaaaga
     4381 aaccagtgat tacatacata tgcatgacgt tgacaagttt tgtatacata cagcaaagag
     4441 tacttaaagc aaaagacctg ggatttatga taaatcaaat gcccacattt ctgggcgttt
     4501 ttgcaaacgg catgatgggt gccttgatat ttgtacatat agcaaacagg tacaaaagag
     4561 agaaatatct gtctgccatg ttcacccttg tgtcgattct atccctttac atgattaggc
     4621 tcattcttaa agaaagtctg ttaagagcac aaagcaatca ggtatggcag gtagagtaca
     4681 gatacatttt gtcccttgta ttcctaatgt tcattatctc attaagccta tctgcgaaat
     4741 ggctgagatt catattctca aacaggtttg tgaggtatct ggcgacgata tcctacaact
     4801 tctacatatg gcatcaaata attgcggtaa agtttaaatt tggcatgggt aagaacaacc
     4861 agattaggat tccgcattgg gaaggcgaca gatcgccgaa ctttacagga gacaaagcct
     4921 ggcagattaa atacacatta atcatactgt ttgcatcaat tatcgttgca accatactta
     4981 catacttctt tgaaagacca atggcaaacc taatattagg aaaaaagaaa tcaaaaaagg
     5041 taaaagagca actaatagag gaatacgtag ttgatagaga gaatttgacc gataatgcga
     5101 tttaaaatat aatggtaaaa aaatcaggat ggtggtgcga tgaggattag aatgtttggc
     5161 agagtcgtgt acatgcctcc agcgtttgtt gcaactgttg ttttaatcat cattttggca
     5221 ttggctatca ttgggtacaa cctgtctgaa aatgacaggg atgcaattac gaaccacacg
     5281 ccaacacccc cgcttaccca atcggaaatt caagtaacag atcctcccct gatatcggta
     5341 catgtaattg gctgtgtcaa agagaaaaaa gttgttaaaa tacccgtagg aagcacagtg
     5401 gaagatgctg ttgttgcagc aggcggtttt accgaggagg ctgacacgga tgcggtaaat
     5461 ctggcttaca ggttgtccga cggaatgcaa ataaggatcc cctcaataga tgacgaggat
     5521 aaaacctgga ttgtttccca tggagataac ggtggtaacg gcggcaacaa tgaaaagatt
     5581 agcattaata aagcgagtgt tgctgaactg gccacattgc cgggcattgg acagactctt
     5641 gccctgagaa tagtggatta cagggagaaa aacggtggat tcaagactct tgaagaaata
     5701 atgaacgttc ctggtataaa ggaagctaaa tttgaaagca taaaagatta cataacttta
     5761 taaatgcaat gaggtgcggc aattgtaaga ccgcacctca tcgtgtggca atatgtaccg
     5821 gttgtgttga catgtattaa aaagccgtgc attaaaatac accggcttgt gaccgctatt
     5881 ttgcaaaatt gttcagtaaa acagagaatg cacctctctt gatgatcttg ttggcatgtt
     5941 cctccaatat agggcgtgta atgcttgcag gcatgtattt cccatcgaat tcggaggcta
     6001 tgtctctaat tttttcaata acgcccgcat tccttttgct taacctgtag gaaatgtaat
     6061 agttgtcatt gtacttgtac aaggatgcag gtacgtttct gctctgtggt atggtaagaa
     6121 ccatggaaat cagattttca aaatcattga aaaccaatac ctcgtaattt tcattgcagc
     6181 tttcgtcact gtaacgcttt tcctttgctt tttcattctc atcgtagaga aggatggaat
     6241 ttaggaaata gtcgttatga tgcaactcgt cagaaaagtc cttgtcttcg ccatcaaaat
     6301 cgaagttttc atcgattggg agaagttctt ttagataagc aagaattttc agtaagtatg
     6361 cgggattaaa atcaaaggtg ctgttgtcta tattctttgc attactggtt cttgtaacag
     6421 taatgatgtg gtcttcctgt ttgtttgatg cgggttcatg aacaactcta cattctgata
     6481 tgtcaaaacc aaactctatt tcagcgtggc tgattacatc aagcaaaagt ctttcgtatt
     6541 gaggagtgtc atacttgaac ttctgggcgt caagatttct ctttttcaaa tcttcagccg
     6601 tcaaagttat tcttattacg tcttcgctga ctttttcaat tttcataggt tggtcctcct
     6661 tttactagca cttttacata cagagctcgc tgttctttat gtacactact attatacttt
     6721 aatttaaaaa aaaagagaag caaaatgatt aacgttatac atattttcta ttaacattat
     6781 gctctcaatt gtcaaataat atgcgattat ctcaacttta cccttgtttt tttctcaaat
     6841 tcatatatac tgtaaatatg ctcccgaaca tcgggtttac ttgaacttag gtaggaggat
     6901 acatttgagg agaaacaaaa gagaggcctt ggtcttgatt gcatttatgt tcataggcag
     6961 cattattgga ggcatagttg gaaaactatt gtcgtatatc ccacatttgg agttcatcga
     7021 taaaattgga aagtcaaaca ttttatccgt aagtttcaat cctctgtttg atattaatgt
     7081 aatacggttt ggttttgatc tgagtttggg tgttaacatc ggtagtatcg tgggcattat
     7141 actggctatt gctatttgga gcaggagata ttgattgaag ttttctgttg cccgacc
//
LOCUS       DAONVY010000039         7166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7909_length_7166_cov_3.727183, whole genome shotgun sequence.
ACCESSION   DAONVY010000039 DAONVY010000000
VERSION     DAONVY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7909_length_7166_cov_3.727183"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /gene="rplF"
                     /locus_tag="PLI11_01845"
     CDS             complement(<1..393)
                     /gene="rplF"
                     /locus_tag="PLI11_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPZ51636.1"
                     /translation="MSRIGKKPVAIPDGVNVTLSNGNVVQVKGPKGSLENKFHPNISI
                     KIEDNNIIVERSSDEKFDRSLHGLTRTLIFNMIEGVTKGFEKTLEINGVGYRAAKQGN
                     KLVLNLGYSHPIEMEEPEGITVEVPAANR"
     gene            complement(450..848)
                     /gene="rpsH"
                     /locus_tag="PLI11_01850"
     CDS             complement(450..848)
                     /gene="rpsH"
                     /locus_tag="PLI11_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPZ51637.1"
                     /translation="MQVTDVIADMLTRIRNAGNAKHETVDIPSSKVKASIADILLEEG
                     YIREVVKIDDGRQGILRITLKYTPTKKHVISGIKRISKPGLRVYAKKDELPRVLGGLG
                     IAIVSTSKGIMTDKKARKLGVGGEVLAYVW"
     gene            complement(873..1058)
                     /locus_tag="PLI11_01855"
     CDS             complement(873..1058)
                     /locus_tag="PLI11_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPZ51638.1"
                     /translation="MAKESMKIKQKREPKYSTRAYNRCQLCGRPRAYLRKFGICRICF
                     RELAYKGQIPGVKKASW"
     gene            complement(1074..1613)
                     /gene="rplE"
                     /locus_tag="PLI11_01860"
     CDS             complement(1074..1613)
                     /gene="rplE"
                     /locus_tag="PLI11_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPZ51639.1"
                     /translation="MLRLKEKYQNEIIPAMMEKFNYKSVMQVPKVEKIVVNMGLGEAR
                     ENPKAIDSAIEDLSLITGQRPVVTKAKKSIAAFKLREGMNVGIKVTLRGARMYAFLDK
                     LVNIVLPRIRDFHGVSPNSFDGRGNYALGIKEQIIFPEIDYDKIDKVRGMDVIIVTTA
                     KTDEEAKELLGLMGMPYRQ"
     gene            complement(1635..1979)
                     /gene="rplX"
                     /locus_tag="PLI11_01865"
     CDS             complement(1635..1979)
                     /gene="rplX"
                     /locus_tag="PLI11_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPZ51640.1"
                     /translation="MHIKKGDTVIVITGKSKGEKGKVIKALPAQNRVVVEGVNLVTKH
                     KKPRGQYDTGGIVTQEAPIHVSNVMLVCPECGKPTRTGNKQIMVDGKAVKVRVCKKKD
                     CGADVFTVDTSK"
     gene            complement(1994..2362)
                     /gene="rplN"
                     /locus_tag="PLI11_01870"
     CDS             complement(1994..2362)
                     /gene="rplN"
                     /locus_tag="PLI11_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPZ51641.1"
                     /translation="MIQTQTRLRVADNTGAKELMCIKVLGGSKKRYANIGDIIVCSVK
                     SAVPGGVVSKGEVVKAVVVRTKKGIRRPDGTHIRFDENAAVIIKDDMNPRGTRIFGPV
                     ARELRSSDFMKIISLAPEVL"
     gene            complement(2380..2637)
                     /gene="rpsQ"
                     /locus_tag="PLI11_01875"
     CDS             complement(2380..2637)
                     /gene="rpsQ"
                     /locus_tag="PLI11_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPZ51642.1"
                     /translation="MAERNIRKTRVGKVISDKMDRTIVVAVETRKTHPLYKKTIKTTY
                     KLKAHDENNEAKIDDVVEVMETRPLSKEKRWRLVSIIEKAQ"
     gene            complement(2661..2876)
                     /gene="rpmC"
                     /locus_tag="PLI11_01880"
     CDS             complement(2661..2876)
                     /gene="rpmC"
                     /locus_tag="PLI11_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPZ51643.1"
                     /translation="MKAKDYKESLRAKSLEDLKKEEMELKEQLFKLRFKHATSHVDNP
                     HEITVIKRNIARVKTIIREKELAALEQ"
     gene            complement(2866..3306)
                     /gene="rplP"
                     /locus_tag="PLI11_01885"
     CDS             complement(2866..3306)
                     /gene="rplP"
                     /locus_tag="PLI11_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPZ51644.1"
                     /translation="MLLPKRVKYRRVHRGRMKGVASRGNTLAHGEFGIQAMECGWITS
                     SQIEAARIALTRHARRGGKVWIKIFPDKPVTKKPAETRMGSGKGSPEYWVAVVKPGRV
                     MFEIAGVPEEVAREALRLAIHKLPIKCKIIARSDAEMEGEANEG"
     gene            complement(3306..3971)
                     /gene="rpsC"
                     /locus_tag="PLI11_01890"
     CDS             complement(3306..3971)
                     /gene="rpsC"
                     /locus_tag="PLI11_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPZ51645.1"
                     /translation="MGQKVNPHGLRVGIIKDWDSKWYANKKNFAEYLVEDTKVREHIK
                     KSLYSAGISRIRIERTANKLIINIDVAKPGIVIGKGGATKDELKAQIEKMTNRNVSIN
                     VFEVKSPDLDAQLVAENIASQLERRISFRRAMKQTMSRSMRAGALGIKTQVSGRLGGA
                     EIARTEHYHEGSIPLQTIRADIDYGFAEADTTYGKIGVKVWIYKGEVLPASSNKSNEG
                     GEA"
     gene            complement(3985..4464)
                     /gene="rplV"
                     /locus_tag="PLI11_01895"
     CDS             complement(3985..4464)
                     /gene="rplV"
                     /locus_tag="PLI11_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPZ51646.1"
                     /translation="MERKRMSTDELLERKDELVESYYAKTPKNKKPLVLMKKERKVLG
                     IGRDRGVAKLNYARISPRKVKIVLDLIKGKDLKEAYAILRYTPKAASEILYKLLKSAE
                     ANAVNNFELDENLLYVADANVSQGPTLKRMRAAARGRGARILKRTSNITIVVKEREE"
     gene            complement(4481..4765)
                     /gene="rpsS"
                     /locus_tag="PLI11_01900"
     CDS             complement(4481..4765)
                     /gene="rpsS"
                     /locus_tag="PLI11_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPZ51647.1"
                     /translation="MSRSVKKGPYVDEKLLKRIIEMNEKNVKQVIKTWSRASTIFPQM
                     VGHTIAVHDGRKHVPVYITEEMVGHKLGEFAPTRTFRGHANKEKSSGLKR"
     gene            complement(4778..5614)
                     /gene="rplB"
                     /locus_tag="PLI11_01905"
     CDS             complement(4778..5614)
                     /gene="rplB"
                     /locus_tag="PLI11_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPZ51648.1"
                     /translation="MAIKTYNPTTPSRRGMTGYTFEEITGKAQVKSLIAPKKRTAGRN
                     SYGRITVRHRGGGAKKHYRIIDFKRDKDGIPATVAAIEYDPNRTANIALLHYADGEKR
                     YILHPAELNVGDKVISGPDADIKVGNALPLERIPVGTVIHNIELKPGKGGQLIRSAGT
                     SAQLVAKEGKEAQIRLSSGEVRIVPITCKATIGQVGNVEHENMKIGKAGRSRWMGKRP
                     HVRGLAMNPSDHPHGGGEGRSPIGMPSPVTPWGKPTIGYKTRKKNKKSDELIVRKRAR
                     RK"
     gene            complement(5629..5994)
                     /gene="rplW"
                     /locus_tag="PLI11_01910"
     CDS             complement(5629..5994)
                     /gene="rplW"
                     /locus_tag="PLI11_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPZ51649.1"
                     /translation="MTDYDIIIRPHVTEKSTMLAAEGKYTFVVDIRATKIDIKRAVER
                     LFNVKVLKVNTVRYDGKKRTRRDASGLNVGYTSRWKKAIVQIDTDPSDVIEYLAEDGK
                     TVTVTKKYKTEIEDFGFNN"
     gene            complement(5994..6626)
                     /gene="rplD"
                     /locus_tag="PLI11_01915"
     CDS             complement(5994..6626)
                     /gene="rplD"
                     /locus_tag="PLI11_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPZ51650.1"
                     /translation="MPKVKVYDMSGNVVEEIELDSNIFGVEINEPAMHMSVKSYLANQ
                     RQGTHATKDRSEVNGGGRKPYRQKGTGRARHGSIRSPLWVGGGVVFGPSPRDYSIKLP
                     KKLKRLALKSSLSTKAQNDDIIVIDKLELEEIKTKRMVQLLKDLDVDETALIVLNGKD
                     ENNDKVVLSSRNIEGVSTAYVNTINVYDILRHSKFIITKDALSKVSEVYA"
     gene            complement(6647..>7166)
                     /gene="rplC"
                     /locus_tag="PLI11_01920"
     CDS             complement(6647..>7166)
                     /gene="rplC"
                     /locus_tag="PLI11_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPZ51651.1"
                     /translation="KTVETDGYDAVRVGYEKIEKRKLNKPDRGLFDKLDMDAYRYLKE
                     FRTEKIDDFEVGQEISIDKMFADGDSVDVSGISKGKGFQGSIKRHGQSRGPMSHGSKY
                     HRGVGSLGSTSDPGRVFKGRKLPGQMGRERVTVQNLKIVKVDAERGLLLIKGAVPGPR
                     GGLLEIRESVKA"
BASE COUNT         1663 a         1804 c         1188 g         2511 t
ORIGIN      
        1 tctgttggca gctggcactt ccactgtaat accttctggt tcttccattt caataggatg
       61 tgaataacct agattcaata cgagtttgtt gccttgcttt gcggctctat atccaacacc
      121 gtttatctcc aatgtttttt caaatccctt tgttactcct tcaatcatat tgaaaataag
      181 agttctggtc aatccatgaa gtgaacggtc aaacttttca tccgatgaac gctcaacaat
      241 aatattgttg tcctcaatct ttatagaaat gttaggatga aacttgtttt ccaatgaccc
      301 tttgggtcct ttaacctgaa ctacatttcc attggataga gttacattta ctccgtccgg
      361 aattgcaaca ggtttctttc ctattctcga cattttcttt cctccgatct taaaattatg
      421 aacaatccaa ttgtcctttt cagattttct taccaaacgt aagccagaac ttctccgcct
      481 acgccaagct ttcttgcttt cttatccgtc ataataccct tcgatgttga aactattgcg
      541 attccaagtc cacccaaaac tctgggcaat tcgtccttct tagcatacac tcttaagccg
      601 ggtttactta ttctctttat tccggagata acatgctttt tcgtaggagt atacttcaga
      661 gttattctta gaattccctg tctcccatca tcgattttaa ctacttccct tatatatcct
      721 tcttccaaca aaatgtcggc aattgaagcc ttaaccttag acgaagggat atctacggtt
      781 tcgtgttttg cattgcctgc atttctgata cgtgtcagca tatcagctat aacatctgtc
      841 acttgcatat ttgctgcctc cttctttata gcctaccagc ttgctttctt tacaccgggt
      901 atctggcctt tatacgctaa ctctctaaag catatacggc atatgccgaa cttccttaaa
      961 tacgcacgag gacgtccgca aagttgacac ctgttgtatg ctctggttga atacttgggt
     1021 tctctttttt gttttatttt cattgattct tttgccacaa tcaatacctc ccgttactgt
     1081 cgataaggca ttcccatgag ccctaacaac tcttttgcct cttcatcggt tttagcagtg
     1141 gttacgatga taacatccat acccctgacc ttgtcaatct tatcatagtc tatctctgga
     1201 aatatgattt gctcctttat tcccagggca tagtttcctc ttccgtcaaa ggaatttgga
     1261 gaaacgccgt ggaagtctct aatacggggc aaaactatgt tcaccaactt atccagaaaa
     1321 gcatacatgc gggctcctct gagagtgacc ttgattccta cattcatacc ttccctgagc
     1381 ttgaaagcag caatagattt ctttgccttg gttacaacag gtctctgtcc cgttatcaat
     1441 gaaagatctt ctattgcaga atcaatggcc tttgggtttt ctcttgcttc tccgagaccc
     1501 atattgacaa caatcttctc aactttagga acctgcataa cactcttata gttgaatttt
     1561 tccatcattg caggaattat ttcgttttga tatttttcct taagccttaa cattttttag
     1621 cctccttgag ttcctcactt gctggtgtcc acggtaaaga cgtctgcacc acaatccttc
     1681 ttcttgcata ctcgcacctt tactgcctta ccgtccacca ttatctgctt gtttccagtt
     1741 ctggtgggtt tgccacattc aggacaaaca agcatgacat ttgatacatg tatgggagcc
     1801 tcttgtgtta caataccacc tgtgtcatac tgacctctag gcttcttgtg ctttgttaca
     1861 aggttaaccc cctcaacaac cactctattc tgagcaggga gagcttttat tactttacct
     1921 ttttcacctt tgctttttcc agtgattacg ataactgtat cacctttttt gatatgcata
     1981 caactacctc cccttatagt acttctggag ccagtgatat gattttcatg aaatcactgc
     2041 ttcttagctc acgagcaaca ggtccaaaaa tacgggttcc tcttggattc atgtcatctt
     2101 ttatgataac tgctgcgttc tcatcaaatc taatgtgtgt tccgtcaggt cttctgatac
     2161 ccttcttggt acgaacaaca acagctttga caacttcacc ctttgaaacg actccgcctg
     2221 gaaccgcact cttaactgaa caaactatta tatcgcctat gttggcgtaa cgcttcttgc
     2281 ttccaccaag aacctttatg cacataagtt ccttggctcc agtattatca gcgactctta
     2341 gtcttgtttg tgtttgtatc atttacaaaa cctcctacac tactgtgcct tttcaataat
     2401 cgaaaccaat ctccagcgct tttccttgct cagaggtctg gtttccataa cttcgacaac
     2461 atcatcaatc tttgcttcgt tgttctcatc gtgtgcttta agtttgtatg ttgtctttat
     2521 ggtcttcttg taaagcggat gtgttttcct ggtctcaacc gctactacta ttgttctatc
     2581 cattttatcg cttatgacct tgccaactct ggtctttctt atatttctct cagccaaaac
     2641 aaactacctc cttccaagga ctactgctca agggctgcca gttctttttc ccttataatg
     2701 gtcttaactc tggcaatatt gcgctttatt actgttattt catgtggatt atcgacatgt
     2761 gatgtcgcat gcttgaagcg aagcttgaac aattgctcct tcaattccat ttcttctttt
     2821 ttaaggtcct ccagactctt tgctctcaaa ctctctttat aatccttagc cttcatttgc
     2881 ttcaccttcc atttcagcgt cactacgcgc aataatcttg cactttatag gaagcttgtg
     2941 tattgccaat ctcaatgctt ctctagcaac ctcttcgggt acgccagcaa tttcaaacat
     3001 tactcttcct ggtttaacaa cagcaaccca gtactctggc gaaccttttc ctgaacccat
     3061 tcttgtttca gctggcttct ttgttacagg tttgtcgggg aatattttta tccacacctt
     3121 gccgcctctt cttgcatgac gtgtaagagc aatacgggca gcttcaatct gactgcttgt
     3181 gatccaacca cattccatag cctgtattcc aaattcacca tgagcaagtg tattgccacg
     3241 ggaggctaca cccttcattc tgcctctatg aactcttctg tattttactc ttttaggtag
     3301 caacattacg cctcgcctcc ctcattactc ttgttgcttg aagcgggtaa aacctcgcct
     3361 ttgtaaatcc aaaccttaac gccgattttg ccataagttg tatcagcttc agcaaaacca
     3421 tagtctatgt cagccctgat tgtctgtaga ggaattgaac cttcatgata gtgttcggtt
     3481 ctcgctattt cagcaccgcc aagacggccg cttacctgtg tctttattcc aagagcacct
     3541 gcacgcatgg acctggacat tgtctgcttc attgctcttc taaaggaaat tcttctctcg
     3601 agctggcttg caatgttttc agcaaccaac tgtgcatcca aatcaggtga ctttacttca
     3661 aaaacattga tgcttacgtt acgattggtc atcttctcaa tctgagcctt gagctcatct
     3721 ttggtcgctc cgcctttgcc tataacaata ccaggttttg caacatctat gttgattata
     3781 agtttgttgg cagtgcgttc tattctgatt ctggaaatac cagcagaata caaggacttc
     3841 ttgatatgct ctctaacctt tgtatcttca accaggtatt cagcaaaatt ctttttgttt
     3901 gcgtaccatt ttgaatccca atctttgatt attccaactc tcagtccgtg aggattaact
     3961 ttctgtccca ttgtttgacc tcctctattc ttctctttct ttaacaacaa ttgttatgtt
     4021 gctggtacgt ttcaatattc tcgcacctct acctcttgca gcggctctca ttctctttaa
     4081 agtgggacct tgagatacgt ttgcatcggc aacatacaac aaattttcat caagctcgaa
     4141 gttgttcact gcgttggctt cagcagactt caagagtttg tatagaatct ctgatgccgc
     4201 ttttggcgta tatctcaaaa ttgcatatgc ttctttcaag tctttgcctt ttatcaaatc
     4261 aagaacaatc ttcacttttc ttggagagat tcttgcatag ttaagtttcg ctacaccacg
     4321 atctctgccg ataccaagta ccttgcgctc tttcttcatc aaaacaagag gtttcttatt
     4381 ctttggtgtc tttgcgtagt agctttcgac aagctcatct tttctttcta gcaactcatc
     4441 agttgacatt ctttttcttt ccattgtcta aacctccact ctatcttttg agaccacttg
     4501 acttctcttt gttagcatga cctctgaatg tcctagtggg cgcaaactca cctagcttgt
     4561 ggcctaccat ttcctcggtt atatacacag gtacatgttt ccttccgtca tgtacagcta
     4621 ttgtatgtcc taccatctgt ggaaatattg tagaggccct ggaccaggtc tttattacct
     4681 gcttaacatt tttctcattc atttcaatta ttcttttcaa gagtttctca tcaacatatg
     4741 gacccttttt aactgatctg ctcataacct gacctcctta cttccttctt gcgcgttttc
     4801 tgacaatcaa ctcatcagac ttcttgttct tcttacgagt cttgtaacca attgtaggtt
     4861 tgccccatgg agtaacagga cttggcatac cgattggtga cctaccttca ccaccaccat
     4921 gtgggtgatc gcttgggttc attgccaaac cacgtacatg aggtctctta cccatccatc
     4981 ttgaacgacc agccttgcca atcttcatgt tctcgtgctc tacgttgcca acctggccaa
     5041 tggtagcctt gcatgtgatt ggtacgattc ttacttctcc ggaagaaagt ctgatttgcg
     5101 cctctttgcc ttcctttgca acaagctgag cgcttgttcc agcacttcta attagctgtc
     5161 caccttttcc aggcttgagc tcaatgttgt gaataacagt tccaacaggt attctctcca
     5221 gaggaagtgc atttcctacc ttgatatctg cgtcaggtcc tgatattacc ttgtctccta
     5281 cattcaattc tgcaggatga aggatgtatc ttttctcacc atcagcgtag tgaaggagag
     5341 ctatattcgc agttctgttt ggatcatact caattgcagc aacagtagct ggaataccgt
     5401 ctttgtctct tttgaaatct ataatcctgt aatgcttctt tgcaccaccg ccgcgatgac
     5461 ggacagttat tcttccataa gaattgcgac ctgcagttct tttctttggt gcaatcaagg
     5521 atttaacctg ggctttgcca gtaatctctt cgaatgtata tccagtcatt cctcttctcg
     5581 atggagtagt cggattataa gttttaatcg ccattattat tctcctcctt agttgttgaa
     5641 gccgaaatct tcaatctctg tcttatactt ctttgttacg gtaacagtct taccgtcttc
     5701 agcaaggtat tcgataacat cggaaggatc tgtatcaatc tgtactattg ccttcttcca
     5761 tcttgatgtg tatcctacat tgagtcctga agcatctcgg cgagttctct tcttgccatc
     5821 gtagcgtacg gtgttaacct tcaatacctt aacgttaaaa agtctttcca ccgctctctt
     5881 aatatctatc tttgtcgccc ttatatcaac tacaaaagtg tacttgcctt cagctgcaag
     5941 cattgtactt ttttctgtta cgtgaggacg gataatgata tcgtaatctg tcattatgca
     6001 tacacctccg ataccttgct taatgcatcc ttggttatta tgaatttgga atgacgaagg
     6061 atgtcatata cgttgattgt atttacataa gctgttgata cgccctcaat gttgcgtgat
     6121 gacagaacaa ccttgtcatt gttttcatcc ttcccattaa gaacgattaa agctgtctca
     6181 tctacatcca aatccttcaa caattgaacc attctctttg tcttaatctc ttccaattca
     6241 agcttgtcaa taacgataat gtcgtcattc tgggcttttg tactgagtga ggacttaagc
     6301 gcaagtctct tcaacttctt aggaagcttt attgagtaat ctcttggtga tggaccaaat
     6361 acaactccac cgcctaccca caaaggtgat ctgatgcttc catgtcttgc acgaccggta
     6421 cccttttgtc tgtatggctt cctgcctcca ccattgactt cgcttctgtc cttggtggca
     6481 tgggttccct gacgctggtt tgcaagatag ctttttaccg acatgtgcat tgccggctca
     6541 tttatttcca ctccaaatat attgctgtca agctctatct cttctacgac attaccggac
     6601 atgtcatata cttttacctt tggcatgtta aaaatcctcc cttacattat gctttgacag
     6661 attctctgat ttcgagtaat ccgcctctag gccctgggac tgcacctttt ataagcaaaa
     6721 ggcctctttc tgcatccact ttcacaatct tgagattctg aacagtgact ctttctctac
     6781 ccatctgacc tggcagcttt ctacccttga acactctacc aggatccgag gttgaaccaa
     6841 gtgaaccgac acctctgtga tattttgaac cgtgtgacat tggtcctctg ctctgtccat
     6901 gtctttttat tgaaccctgg aatcctttac ccttggaaat tccactaaca tccacagagt
     6961 ctccatcggc aaacatctta tcaatactga tttcctgacc tacttcgaaa tcatcgattt
     7021 tctctgttct gaactctttc agatatctgt aggcatccat gtcaagctta tcgaacaggc
     7081 ctctgtcagg cttgtttagt ttgcgttttt caatcttttc gtaacctact cttacagcat
     7141 cgtatccgtc ggtctcaact gtcttt
//
LOCUS       DAONVY010000040         7140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7943_length_7140_cov_4.111503, whole genome shotgun sequence.
ACCESSION   DAONVY010000040 DAONVY010000000
VERSION     DAONVY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7943_length_7140_cov_4.111503"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLI11_01925"
     CDS             complement(<1..327)
                     /locus_tag="PLI11_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPZ51652.1"
                     /translation="MNFSYLVSIFGQLSDGLKFTVGLFFVTIIFSVPLGLVLSFIRVG
                     SHKIIQRILGGYIWLMRGTPLILQLYFFYYGLSLVTSIQLSRFNAAIVAFILNYAAYF
                     CEIFRGG"
     gene            complement(327..1160)
                     /locus_tag="PLI11_01930"
     CDS             complement(327..1160)
                     /locus_tag="PLI11_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPZ51653.1"
                     /translation="MKKTLLIVTVLIIVLASACTPNIQKNFVVLEDYFAEEEYGIGFR
                     NTDIALGLEVQKHLDEMYEDGTLIEISKKWFGKDTYLKDSDFIEESQAPEGDDSLDKI
                     LDKGKLIVGLDDSFPPMGFRDDKNNIVGYDIDLAKEVAKRMDVELVLQPIDWDSKELE
                     LQSGKIDCIWNGMTITEERVEAMYFVKPYIANRQIVIVGENSDIKTKADLNGKKIGLQ
                     KGSSALNAVKADDVYSKIGEIIEYQNNVDAFNDLKVGRIDALVVDEVAGRYMIEKDKD
                     K"
     gene            1323..1730
                     /locus_tag="PLI11_01935"
     CDS             1323..1730
                     /locus_tag="PLI11_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HPZ51654.1"
                     /translation="MKIVVIDGQGGRMGKMLVEKIKPIALDHEIMAIGTNSIATSAML
                     KAGADYGATGENPIVWNCKDADIILGPIGIIIANALHGEISPKMAQAVGECKAHKILV
                     PVNKCNLKVMGVSDMSLSDYIDLAVDEVKTLLK"
     gene            complement(1711..2286)
                     /locus_tag="PLI11_01940"
     CDS             complement(1711..2286)
                     /locus_tag="PLI11_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ51655.1"
                     /translation="MTNLEKSILRQIEKKGIVLIAIDGPSGSGKSTFAKRLAEKYDAN
                     VFHMDDFFLRPEQRTKERYEEIGGNIDYERFHEEVLMNINRGFPFHYRPYSCSLGILV
                     DKKEVTFKNVNIIEGVYSMHPMLLSYYDIKVFLTAPKEVCIERIRKRSGDVLLKRFVN
                     EWIPMEQNYFSNFKIKENCDFIINGVTLEES"
     gene            complement(2283..2831)
                     /locus_tag="PLI11_01945"
     CDS             complement(2283..2831)
                     /locus_tag="PLI11_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPZ51656.1"
                     /translation="MKRRDVYNLVLAAMFLALAFVLPQIMKSIPFFVSANLGTKILPM
                     HIPVLLCGFICGYYYGLSVGFIAPLLASLITTMPPMYPVAISMAFELAAYGLVAGLMY
                     KATSKLKINYVLKIYIDLITAMLAGRVVMGVVNMLLLTNAKYGLNVFLADAFVNALPG
                     IIIQLILIPAILIILRKAKYIK"
     gene            complement(2950..5040)
                     /locus_tag="PLI11_01950"
     CDS             complement(2950..5040)
                     /locus_tag="PLI11_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51657.1"
                     /translation="MYHNRKVLSFIAAILYIIVLCSSGIIVAGQNDNPSATLPPRPVL
                     DENHPVNTMDVRPDTWVATDGLGRTLSGYNEVGPKREDRFVGLFYWTWHGSHAKSTVP
                     RNVQQLLDSLTPEEAEKAKNDYKHPVWERRTGYHFWNEPIFGYYSTNDKYVLRKHAEM
                     LADAGVDVVIFDNTNGSFLWQDSYTALMETWSEARQQGVNTPQVTFMLNFGPLETTAE
                     MLVNIYKDIYQQEKFKDLWFYWDGKPFIMAHEKALDARNPLQAEILEFFTFRNNDPSY
                     FTKTENAKPNSWGWLSTYPQCKYNVDKNGNVEQMTVGVAQNATTTKLTAMNGKNVLGR
                     SYGHDGYSYSYTYRGKEIVVNSDIPDSKLYGINLQQQFDYAIENDPKFIFVTGWNEWV
                     AIRFDEWSGVPNGMADQFNDEYSRDLEPSKGDLKDHYYYQLVENIRRYKGVSEPEEID
                     VYKKIDIFGDLSQWDDIASFNHYIKNTWERNDRGYLTKTYKNDTMRNDIRRAKVAFDE
                     NNIYFYVETVDNLTPYTDPAWMRLFIDTMPATEDSKDWEEIEFVVNRQNPTDSIAYLE
                     RSKGGWDWEVVQEVRYSVMDNVLQIEIPRKALGLDDTDAPLFNFKWADNMQEDGDILD
                     FYVNGDVAPGGRFMFHFNTKPVKNADIADKETKKGGIQPVVIVSIISASAVVAVAVAA
                     VLIVKKSNASKVSS"
     gene            5242..6606
                     /locus_tag="PLI11_01955"
     CDS             5242..6606
                     /locus_tag="PLI11_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPZ51658.1"
                     /translation="MSLELTPESAVKIQNLSDLCINNGSIDPSLYTKYNVKRGLRDLQ
                     GKGVLAGLTNISEIISYEVVDGKEVPCDGKLYYRGIAVEDIVSGCVDDKRFGYEEVTY
                     LLIFGKLPNKQELEDFNNLLSEFRTLPTSFVRDIIMKAPSSDMMNTLARSVLTLYAYD
                     DKADDISIPNVLRQCLELVSLFPLLSIYGYHAYNHYVNKESLFIHPPQPELSTAENIL
                     YTLRKDSKYSVLEARILDVALILHADHGGGNNSTFTTHVVSSSGTDTYSVVAASLGSL
                     KGPRHGGANIKVVQMFEDLKNNVKNWEDEEEISRYLMALLNKQAFDKSGLIYGMGHAV
                     YSKSDPRAKVLKSFVKSLSEEKGLDEEYKLYTLVERLAPEIISKERKIYKGVSANIDF
                     YSGFVYKMLDLPIELYTPIFAISRIAGWSAHRIEEIINAGKIIRPAYKNVSDKIQYVK
                     LNDR"
     gene            complement(6636..>7140)
                     /locus_tag="PLI11_01960"
     CDS             complement(6636..>7140)
                     /locus_tag="PLI11_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPZ51659.1"
                     /translation="NEDSFEYGNLVDNIPEWNPTGERKKVLKAEESEPDSVTLSTGVL
                     KKNELEALLNALPFDITFVDKNGLVKYFSQSAERIFARTTTVLGRHVTNCHPPTSVHV
                     VTQIVEDLESGKKDHEDFWIRMGDKYVFIRYFAVRDNDNNFLGVLEVTQNIAPIQEIT
                     GEKRLMS"
BASE COUNT         2171 a         1455 c         1203 g         2311 t
ORIGIN      
        1 accacctctg aatatttcgc aaaaatatgc agcatagttt aggataaaag caactatcgc
       61 tgcattaaac ctgctcagct gaatggacgt taccaatgat agaccgtaat aaaagaaata
      121 cagctgaagt ataagggggg taccccgcat aagccagata tatcccccta aaatacgctg
      181 gataatttta tgactaccga ctctaataaa cgacaataca agtcccagcg gtactgaaaa
      241 tattatggtt acgaaaaata gtccaacagt aaacttcaaa ccatccgata gctggccgaa
      301 gatgctgacc aagtaagaaa aattcattat ttatctttat ccttttctat catatatctt
      361 cctgcaactt catccactac aagtgcgtca attctaccta ccttcaaatc attaaatgca
      421 tcaacattgt tctgatattc aatgatttca ccaatctttg aatatacatc gtcagcttta
      481 acagcattca aagcagaaga acctttttgg aggccaatct tcttgccatt taagtctgcc
      541 tttgtcttta tatctgagtt ctcaccaaca attacaatct gtctgtttgc tatgtatggt
      601 tttacaaaat acattgcttc aactctttct tcggtgattg tcattccatt ccagatacag
      661 tcaatcttgc cgctctgaag ctcaagttcc ttagaatccc agtctatagg ctgcaaaaca
      721 agctccacat ccattctctt tgcaacttct tttgcaaggt caatatcata acccacaata
      781 ttattcttgt catctctgaa gcccattggt ggaaatgagt cgtcaagacc aacaatcaac
      841 tttcctttat ccaatatctt atctagagaa tcgtctcctt caggtgcctg gctttcttca
      901 ataaaatcag aatctttcaa atatgtatcc ttaccaaacc atttctttga gatttcgatc
      961 agtgtgccgt cttcatacat ctcatcaagg tgcttttgaa cctcaaggcc caaggcaatg
     1021 tctgtgtttc taaatccaat gccgtattct tcttctgcaa aatagtcttc aagaacaaca
     1081 aagtttttct gaatatttgg agtacatgca gaagccaata caattatcaa tacagttaca
     1141 ataagcaatg ttttcttcat gattataaca acccttccac tttagtatgt taattcgcta
     1201 atttattaaa ccagcttaaa tagtatatca tattatccag aattgtaaat gagtaaaatt
     1261 aaatatttga taaaaaggct attgtatgag attatatata aaaaattaca ggagtgatga
     1321 atttgaaaat tgttgttata gatggtcagg gaggaagaat ggggaaaatg ctggtggaaa
     1381 aaataaaacc aatagctctt gatcatgaaa tcatggctat tggtacaaac agtattgcca
     1441 catccgccat gctcaaggca ggagcggact acggtgcaac cggtgaaaac ccaattgttt
     1501 ggaactgcaa ggatgcggat ataattcttg gaccgattgg aatcattatt gccaatgctc
     1561 tccacggaga aatatctcca aagatggcac aggcagttgg tgaatgcaaa gcacataaaa
     1621 tacttgttcc agtaaacaag tgcaatctta aagttatggg agtcagtgac atgtccctga
     1681 gcgattacat tgatcttgct gtggatgagg tcaagactct tctaaagtaa ctccattgat
     1741 tataaaatca caattctcct ttattttaaa attgctaaaa taattctgtt ccatgggaat
     1801 ccattcattt acgaatcttt taagcagaac atctccactt cttttcctaa ttctttcaat
     1861 gcaaacttct ttaggagctg tcaaaaatac ttttatatcg taatatgata aaagcatggg
     1921 gtgcatgcta tacacccctt caattatatt cacattctta aaagtaactt ctttcttgtc
     1981 cacaagtatt cctaaagaac agctgtaggg cctgtagtgg aaaggaaacc ctctatttat
     2041 attcatcaat acttcctcgt gaaacctctc ataatcgata ttccctccaa tttcttcata
     2101 tctttccttt gttctttgtt caggtctcag aaagaagtca tccatatgaa atacatttgc
     2161 atcatatttt tccgctaacc ttttagcaaa ggtcgacttt ccacttccac ttggtccgtc
     2221 aattgcaatt aaaactattc cttttttctc aatctgtctt agaatacttt tttcaagatt
     2281 agtcatttga tatattttgc cttcctcaat attattaata ttgcaggtat taatattaac
     2341 tggataatta taccaggcaa tgcatttaca aatgcatccg ccaggaatac atttaaccca
     2401 tactttgcat ttgttaatag taacatatta acaacaccca ttacaaccct gcctgcaagc
     2461 attgctgtta tcaggtctat gtagattttt aaaacataat ttatctttag ttttgatgtt
     2521 gccttgtaca taagtcctgc tacaagtccg taggcagcca gctcaaacgc catggaaatt
     2581 gccacggggt acataggagg cattgttgtt atgagacttg ccagcaaagg agctataaag
     2641 ccgacagaaa gcccatagta atatccacag ataaatccac ataaaagaac cggaatatgc
     2701 atgggaagta tttttgtacc cagatttgct gacacaaaaa acggtatgga tttcataatc
     2761 tgcggtagaa cgaatgccag agccaaaaac atggcagcaa gaaccaaatt gtatacatct
     2821 cttcttttca taaaacacta tcacctctga aaaatataaa aagataccat gaagtatcac
     2881 tttccttggg aaagcatata ccttcatggt atcattgcag acaatattat atcactcaaa
     2941 gattaaaaat caagaggata ccttacttgc gtttgatttt ttaacaatta acaccgcagc
     3001 aactgcaacg gccacaacag ccgatgcgga tattattgat actataacta ccggctgtat
     3061 gcctcctttt ttggtttcct tatcagcaat atcagcattc tttacaggct ttgtgttaaa
     3121 gtggaacatg aatcttccac ctggagccac atcaccattt acatagaaat caagaatgtc
     3181 tccatcttcc tgcatgttgt cagcccattt gaagttgaat aaaggtgcat ctgtatcatc
     3241 aagtcctaga gcctttctgg gtatttcaat ctgtagtaca ttatccatta cagaatatct
     3301 gacttcctgt acaacctccc aatcccatcc gcctttgctt ctttctagat atgcaataga
     3361 atcggttggg ttctgcctgt ttaccacaaa ctcgatttct tcccaatcct ttgaatcttc
     3421 tgtagcaggc attgtatcaa taaaaagtct catccatgca ggatcggtat atggcgtaag
     3481 gttatcaaca gtttccacat agaaatatat gttgttttca tcaaaagcta cctttgctct
     3541 tctaatatcg tttctcatag tatcattctt gtatgtcttt gtaaggtagc ctctatcatt
     3601 acgttcccat gtgtttttga tgtagtgatt gaaagatgcg atatcatccc actgtgacaa
     3661 atcaccgaag atatctattt tcttgtatac atctatttcc tctggctcgc ttactccctt
     3721 gtacctacga atattctcta caagttggta gtaatagtga tctttcaaat ctcctttgga
     3781 aggttcaaga tcccttgagt actcatcatt gaactggtct gccattccat taggaacacc
     3841 gctccattca tcaaatcgaa tagcaaccca ttcattccat ccagttacaa atatgaactt
     3901 tggatcgttc tctattgcat aatcaaactg ttgctgcaag tttataccgt aaagctttga
     3961 atcgggtatg tcggaattaa ccactatctc cttacctcta taagtatagc tgtaagaata
     4021 tccatcatga ccgtagcttc ttccaagcac attcttgcca ttcatggcag tcagctttgt
     4081 ggtagttgca ttctgggcaa caccaacagt catttgttcc acattgccgt ttttatccac
     4141 gttatacttg cactgcggat aagtggacaa ccagccccag ctgttgggct ttgcattctc
     4201 tgttttagta aaatatgatg gatcattatt tctgaatgtg aaaaactcca aaatttcagc
     4261 ctgtagcgga tttcttgcgt caagtgcttt ctcatgcgcc ataatgaatg gttttccgtc
     4321 ccaatagaac cacaaatctt taaatttttc ttgttgatat atatctttat atatatttac
     4381 cagcatctct gcggttgttt ctagtggacc aaagttaagc atgaaggtaa cttgaggtgt
     4441 gttaactccc tgctgtcttg cttcagacca ggtttccatt aacgcagtat aggaatcctg
     4501 ccatagaaat gaaccattgg tgttgtcgaa tataactacg tcaacgcctg cgtccgcaag
     4561 catttcagca tgttttctta atacatactt gtcatttgtt gaatagtaac cgaaaattgg
     4621 ctcattccag aaatgatatc ccgttctacg ctcccataca ggatgtttgt aatcattctt
     4681 ggctttctct gcttcttcgg gtgttagtga atcaagtagc tgctggacat tccttggcac
     4741 agtgctcttt gcatgtgatc cgtgccatgt ccagtaaaat aatccaacaa atctatcttc
     4801 tcttttggga ccaacctcgt tatatcccga cagtgttctt cccaaaccat ctgtagccac
     4861 ccaagtgtcc ggccttacat ccattgtgtt taccggatga ttttcatcca gtactggcct
     4921 tggaggaagt gtagctgatg gattatcatt ttgtcctgca acaatgatac cagaactgca
     4981 cagtacaatt atgtataaaa ttgctgcaat gaaagacaaa acttttctgt tatgatacat
     5041 tttcagaacc tcccgatttt ttctgtatta tattatttat tacatagtta agtcaaccct
     5101 tacttcttta tgccgagttt gacttttcat ttttttcttt actcactttt ggttttatgg
     5161 taatatttaa atgtttactt ttttttattc atctctctta tcccaaaaat aacttttaaa
     5221 acaaaagaaa gtggtgatat tatgtcatta gaacttactc ctgaatctgc tgttaaaata
     5281 caaaatttat ctgacttatg cattaacaac ggatcaatag atccaagttt atacaccaag
     5341 tataacgtta agagaggatt aagagatttg cagggcaaag gagttcttgc aggactgaca
     5401 aacataagcg aaattatttc atatgaagtg gtagacggaa aagaagtacc ctgtgatgga
     5461 aagttgtatt atcgaggcat agctgttgaa gatatcgtat caggttgtgt tgatgataaa
     5521 cgatttggtt atgaggaggt aacttattta ctcatattcg gcaagcttcc aaacaaacaa
     5581 gaattggaag attttaacaa tttgttatct gaattcagaa cattgcctac ttcttttgta
     5641 agggatatca ttatgaaggc tccaagcagc gacatgatga atacccttgc cagaagcgtt
     5701 ttaactttgt atgcgtatga cgacaaggca gatgacatat caataccgaa tgtattgagg
     5761 cagtgtcttg agcttgtctc actgtttcct ctattatcga tatatggata ccatgcttac
     5821 aaccattatg taaacaagga aagtttgttt attcatcctc cacagcctga attatcaact
     5881 gctgaaaaca ttctctatac attgagaaaa gacagcaagt attccgtgct tgaagcaaga
     5941 attcttgacg tagcactgat actacatgcg gaccacggcg gaggtaacaa ctctacattt
     6001 acaactcatg ttgtatcatc ttcaggaact gatacttatt cagtagttgc tgcctctctt
     6061 ggttccttga aaggtcctag acatggcggt gcaaatataa aagtcgtgca aatgtttgaa
     6121 gaccttaaga ataacgtaaa gaattgggaa gacgaagaag aaataagcag atatctaatg
     6181 gcgctattaa acaagcaagc ctttgataaa tcaggattga tttatggtat gggacacgca
     6241 gtatactcca aatctgatcc aagagcgaaa gtcctgaaga gttttgtaaa gagtttgtcc
     6301 gaggaaaaag gacttgacga agaatacaag ctatacacgc tggtagaaag acttgctcct
     6361 gagattatta gcaaagaaag aaagatttac aaaggtgtaa gtgcaaatat tgatttctac
     6421 agcggctttg tttacaaaat gcttgacttg cctatagaac tttacacacc tatttttgca
     6481 atatcaagaa ttgcaggctg gtccgcacat aggattgaag aaattattaa tgcaggaaag
     6541 attatcaggc ctgcatacaa gaatgtatcg gataagatac aatacgttaa acttaacgac
     6601 agataaagaa agacctttgt aaaggtcttt cttttttatg acattaatct tttctctcct
     6661 gttatttctt gtattggagc tatgttctgc gttacctcaa gtactccaag gaagttgtta
     6721 tcattatctc gaactgcaaa gtacctgata aatacatatt tgtcccccat tcttatccaa
     6781 aaatcctcat gatctttctt tcctgattcc aaatcttcaa caatttgagt gactacatgg
     6841 acggatgtag gtggatggca gttagtaaca tgtcttccca gtacagtggt tgttcttgca
     6901 aagattctct ctgcactctg tgagaagtac tttacaagac catttttgtc aacaaatgtg
     6961 atatcaaatg ggagtgcatt taataatgct tcaagttcat tctttttaag aacaccagtt
     7021 gaaagtgtca ctgaatctgg ttccgattcc tctgctttca atactttttt tcgttcccca
     7081 gtaggattcc attcaggaat attatcaacc agatttccat attcaaagct gtcctcattt
//
LOCUS       DAONVY010000041         7139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7945_length_7139_cov_3.751553, whole genome shotgun sequence.
ACCESSION   DAONVY010000041 DAONVY010000000
VERSION     DAONVY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7945_length_7139_cov_3.751553"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLI11_01965"
     CDS             <1..244
                     /locus_tag="PLI11_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51660.1"
                     /translation="AAIKQKKDPLLGLKENVIIGKLIPAGTGMSRYKDIEIMVERLDD
                     GVEDTIPTKQIADEEDIFEEVLVDDSGMPDVDDFDE"
     gene            365..778
                     /gene="rpsL"
                     /locus_tag="PLI11_01970"
     CDS             365..778
                     /gene="rpsL"
                     /locus_tag="PLI11_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPZ51661.1"
                     /translation="MPTFNQLVRKGRKTFQQKSHAPALQKGYNILNKRSTDVSSPQKR
                     GVCTVVRTTSPKKPNSALRKIARVRLTNGMEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTLDTAGVANRKQGRSKYGAKAPKK"
     gene            915..1385
                     /gene="rpsG"
                     /locus_tag="PLI11_01975"
     CDS             915..1385
                     /gene="rpsG"
                     /locus_tag="PLI11_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPZ51662.1"
                     /translation="MPRKGYIAKREVLPDARYNEKLVTKLINNIMLDGKKGTAEKICY
                     KAFDIVQEKTGRDALEVFKEALNNIMPQLEVKARRVGGATYQVPVEVRPERRQTLGLR
                     WLVVYARNRGERTMAERLANEILDALNQTGGAFKRKEEMHRMAEANRAFAHYRW"
     gene            1467..3539
                     /gene="fusA"
                     /locus_tag="PLI11_01980"
     CDS             1467..3539
                     /gene="fusA"
                     /locus_tag="PLI11_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPZ51663.1"
                     /translation="MPRSYSLENTRNIGIMAHIDAGKTTTTERILYYTGKTHKMGETH
                     EGDATMDFMVQEQERGITISSAATTAIWKNYRVNIIDTPGHVDFTVEVERSLRVLDGS
                     VTLFCAKGGVEPQSETVWRQADKYKVPRIAYVNKMDITGANFFRVLDMIRTRLGANPV
                     AIQLPIGSEDTFIGIIDLVKMDALQYKGDKGEIVEEIDIPADMMDLAVEYRGKMIEAL
                     ADFDENIMMKYLEGEEITEEEIKAAIRNATLAVQIIPVMCGSSYRNKGVQQLLDAVID
                     YLPSPLDIPAIKGVNMDGEEDERHPSDDEPFSALAFKIVTDPYVGKLCFFRVYSGVLE
                     SGSYVMNMTKGSKERIGRILLMHANHREEVDKVYAGDIAAAVGLKDTSTGDTLCSDKA
                     PIILEKMEFPEPVISVAVEPKTKAGQDKMGVALQKLAEEDPTFKVHTNVETGQTIISG
                     MGELHLEIIVDRLLREFKVEANVGNPQVAYKEGIRKAVKVEGKFVRQSGGRGQYGHCV
                     IEVEPKERGTGYEFVNKIVGGVIPKEYIPAVDAGIKEALQSGILGGYPVVDIKVALVD
                     GSYHEVDSSEMAFKIAGSMAIKEALKNAEPVLLEPIMKVSINTPEDYLGDVISSVSAR
                     RGRIEGMEDNAGSKDIQAVAPLAELFGYTTSLRSMTQGRGSFTMQPSHYEEVSKNIQE
                     DLLSRKSK"
     gene            3673..4869
                     /gene="tuf"
                     /locus_tag="PLI11_01985"
     CDS             3673..4869
                     /gene="tuf"
                     /locus_tag="PLI11_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPZ51664.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKYLALIGNAEV
                     KAYDQIDNAPEEKQRGITINTSHVEYQTENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILLVSGADGPQAQTREHILLARQVNVPYIVVFMNKCDMVDDPELLDLVEMEVRELLSK
                     YDFPGDDVPVIRGSALMALEAGDDPTDSAYAPIKELMEAVDTYIPEPERETDKPYLMP
                     VEDVFTITGRGTVATGRLERGTIKVGDQAEVVGLKDETMSTVITGIEMFRKQLDEAYA
                     GDNIGALLRGITREDIERGQVIAKPGTIKSYTKFKGQVYVLTQNEGGRHKPFFSNYRP
                     QFYFRTDVTGIIQLPEGVDMCLPGDHVEITVELITPIAMEEGLRFAIREGGKTVGSGT
                     VTAIID"
     gene            5036..5332
                     /locus_tag="PLI11_01990"
     CDS             5036..5332
                     /locus_tag="PLI11_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51665.1"
                     /translation="MKAKYILVFLLAIMMIFSFAACRNGGLLDPSPGITISPNVTDDG
                     DGDMSPDLSPDLSPDLSPDVSPGAGGSPGAGGPGTSPEGSPGGNGGANGASKQV"
     gene            5485..6183
                     /locus_tag="PLI11_01995"
     CDS             5485..6183
                     /locus_tag="PLI11_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPZ51666.1"
                     /translation="MIKVLICDDNRQITSILEEYCKREGYKVKVAYDGEEALEFFSSC
                     MPDIILLDVMMPKLDGFYVCKEIRKKSNVPVIMVTARGEDFEKIMGLEIGADDYIVKP
                     FSGGEVMARIRAILRRCSYEGRTEGHELKIANLYINMDSYEVMIDDEEVSLTKKETEI
                     LWLLASNSGKVFSRENLLDKLWGYDYYGDMRTVDSHIKRLRAKIDAFPHESWDVTTIW
                     GVGYRFTEYKEGKG"
     gene            6186..>7139
                     /locus_tag="PLI11_02000"
     CDS             6186..>7139
                     /locus_tag="PLI11_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPZ51667.1"
                     /translation="MRKKLVFKLTFYFTIVLLILSIGIGIVFTILFRNHTVDLYKRNL
                     VNSAVGVSETLSEFTFRMQYSRFPLFDFPNRNNFYFSNDAQVYISFLNSITMANVWII
                     DRDYNFITTGTSYYDGSRVYTFSELPHNAEKVIQKVFEGETTFSEVFGNFFETPTLTV
                     GVPIVSQNGEVIGVVLLHSPIDYMQKAIEQGIKTLVISILLALVLSVILSFSFAWAFT
                     KPMDKIRNVTLELAKGNYEVKTDVSQDDEIGDVAKAVDILSERLNEASREREKLDKMR
                     KDFVANISHELRTPVTVIRGSVEALHDKVVTEPKQVEEYYSH"
BASE COUNT         2271 a         1279 c         1754 g         1835 t
ORIGIN      
        1 agcagccatc aagcagaaga aggatccatt gcttggtctg aaggagaacg taatcatcgg
       61 aaaactcatt cctgctggaa ccggcatgag cagatacaag gatattgaga ttatggtgga
      121 aagactggat gatggtgtgg aggatactat ccccacaaag cagattgccg acgaagagga
      181 tatttttgag gaagttctcg tagacgattc gggcatgcct gatgtcgatg attttgatga
      241 ataaagaaaa taacttgaca acataatcat attggtgtag tattagttgg ctgtgcaggt
      301 ggcttttttg acacctgcaa agcttaatta ataaaggata taaaactggg aggtgaaagc
      361 caagatgcca acatttaatc agctggttag gaaaggtaga aaaacatttc agcaaaagtc
      421 acatgcgcct gcattgcaga agggctacaa cattcttaac aagcgcagta cggacgtaag
      481 cagtcctcag aagaggggtg tttgcactgt tgttaggaca acttctccaa agaagcccaa
      541 ctccgctctg cgtaagattg cgagggtgcg tcttacaaat ggaatggaag ttacagcata
      601 cattccagga attggacaca acctgcagga acatagcgtt gttctgataa gaggcggaag
      661 agtaaaagat ttgccaggtg taagatatca cattattaga ggtacattgg ataccgcagg
      721 tgttgcaaac cgtaaacagg gaagatcaaa atacggcgcc aaagcaccta agaaataaca
      781 gaagaaatga aacgggacat ctcagaaggt gttaggtgtc ttttatatat atataattga
      841 atagatacca gcactgacat cattcattga tgagtaccga tgaattttta tctcatgaaa
      901 aggagggaag gaaagtgcct agaaaaggat acattgctaa aagggaagtt ttacccgacg
      961 caagatacaa tgaaaaactc gttacaaaac ttatcaacaa catcatgctt gacggaaaaa
     1021 aaggtacggc agagaagatt tgctataaag cttttgacat agttcaggag aagactggaa
     1081 gagacgctct tgaagtattt aaagaggctt taaataatat tatgccacaa ttggaagtaa
     1141 aggccagaag agttggtggt gcgacttatc aggttccagt agaagtaagg cctgaaagaa
     1201 ggcagactct tggtctcaga tggcttgttg tttatgcaag aaatcgtggt gaaagaacca
     1261 tggctgaaag acttgcgaat gaaatacttg atgcattgaa tcaaacaggt ggtgcattca
     1321 agcgtaagga agaaatgcac agaatggcag aggcgaaccg tgcatttgct cactatcgct
     1381 ggtaattttg tgctaataaa tttattgaat caaggttatc atacaattag tatttttgaa
     1441 attggaattt taagaaggag gctccaatgc caagaagtta ttcacttgag aatacaagaa
     1501 atataggcat catggcacac attgatgcag gaaaaacaac cacaaccgaa cgaatactct
     1561 actacacggg aaagacacac aaaatgggtg agacccacga aggtgacgca accatggact
     1621 tcatggttca ggagcaggaa agaggtatca ccatatcttc cgctgctact acagccattt
     1681 ggaagaatta cagagtaaac ataattgaca cacctggtca cgtggacttc accgtggaag
     1741 tagaaagatc cttgagagta ttggacggta gtgttacctt attctgtgca aaaggaggtg
     1801 tagaacctca gtccgaaact gtctggagac aggctgacaa gtacaaggta ccaagaattg
     1861 cctatgtaaa caagatggac ataaccggtg caaacttttt cagggttttg gacatgatta
     1921 ggactagact tggtgcgaat cctgttgcga tccagttgcc aattggcagc gaagatacct
     1981 ttataggtat aatcgacctc gttaaaatgg acgctcttca atacaagggc gacaagggtg
     2041 aaatcgttga agaaatcgat atcccagcgg acatgatgga cctggctgtt gaatacaggg
     2101 gcaaaatgat tgaagcactt gcggatttcg atgaaaatat aatgatgaag tacctggagg
     2161 gcgaggaaat taccgaagag gaaatcaagg ctgctataag aaatgcgaca ctggctgtac
     2221 agattattcc tgtaatgtgt ggctcctcct acagaaacaa gggtgttcag caactgctcg
     2281 atgccgtcat tgactacctg ccatcaccgt tggatatccc tgctatcaag ggagtcaata
     2341 tggatggcga ggaagatgaa agacatccta gcgatgacga acccttctca gcccttgcgt
     2401 tcaagatcgt gactgaccca tacgtaggaa agctctgctt cttcagagtt tattcaggtg
     2461 ttcttgaatc cggttcctat gtcatgaaca tgaccaaggg aagcaaggag cgtatcggaa
     2521 gaattcttct gatgcatgcg aatcacaggg aagaggttga caaggtctat gccggtgata
     2581 tagccgctgc agttggtctg aaggatacat ccacaggcga cacgttgtgc tcagacaaag
     2641 cgccgattat ccttgagaag atggagttcc ctgagccggt tatctctgtt gctgttgagc
     2701 ctaagacaaa ggctggacaa gacaagatgg gagttgcgct gcagaagctc gctgaagagg
     2761 acccaacatt caaggttcat actaatgtgg aaaccggtca gacaatcatt tcgggtatgg
     2821 gtgaactgca ccttgagatt attgttgaca gactgcttcg ggagtttaag gttgaagcaa
     2881 acgtaggaaa tccccaggtt gcatacaaag agggtattag aaaagctgtc aaggttgaag
     2941 gcaagtttgt ccgtcaatcc ggcggtagag gccagtacgg tcactgcgtt atcgaggttg
     3001 aacccaagga aagaggaact ggttacgagt ttgttaacaa gattgttggc ggtgtaattc
     3061 cgaaagaata tatcccggca gtggatgcag gaatcaagga agctttgcaa agtggtatac
     3121 tgggtgggta tccggtagtc gacataaagg ttgcactggt tgacggttca taccatgagg
     3181 ttgactcatc ggagatggcc ttcaagattg caggttccat ggcaatcaag gaagctttga
     3241 aaaatgcgga accagttttg cttgaaccta ttatgaaggt tagcatcaac acacctgagg
     3301 attaccttgg tgatgtcata agctcagtgt cagcccgcag aggccgcatt gagggcatgg
     3361 aagacaacgc tggctccaag gatatacagg cagtagctcc tcttgcggaa ctgtttggct
     3421 ataccacatc actgcgttca atgacacaag gccgtggctc atttacaatg cagccaagcc
     3481 actacgagga agtgtcaaag aacatacagg aagatttgct ttcaagaaag tcaaagtaat
     3541 ataatttatg tataaaattg aaggaaatag ggaaatataa aatagtaaca aaaaaaactt
     3601 gcctattttc taaatattat gtaaaataat aaaaacttta ataattatga gaattttaag
     3661 gaggaaataa aaatggcaaa agctaagttt gaaagaacaa agccacatgt aaacattggt
     3721 acaattggac acgttgacca tggtaaaaca acattgactg ctgcaattac aaaatatttg
     3781 gctcttatag gtaatgcgga agtaaaggca tatgaccaaa ttgacaatgc tccagaggag
     3841 aaacaaagag gtatcacaat aaatacttcc cacgttgagt atcagacaga aaacagacac
     3901 tatgcgcacg ttgactgccc cggtcacgct gactatgtta agaacatgat tactggtgct
     3961 gctcaaatgg atggtgcgat tttgctggta tccggcgctg acggcccaca ggctcagaca
     4021 agagaacaca ttctgcttgc tcgtcaggtt aacgtaccat acatagttgt tttcatgaac
     4081 aagtgtgata tggtagacga cccagagctt cttgaccttg ttgaaatgga agttcgtgag
     4141 cttctcagca agtatgactt cccaggagac gacgtgccag ttatcagagg ttctgctttg
     4201 atggctctcg aagctggaga cgacccaaca gattctgctt acgctccaat caaagagctt
     4261 atggaagcag tagacactta cattccagag ccagagcgtg aaactgacaa gccttacctt
     4321 atgcctgttg aggacgtgtt cacaatcaca ggtcgtggaa cagttgcaac tggcagactt
     4381 gagagaggaa ctatcaaggt tggcgatcag gctgaagtcg ttggtctgaa agatgagacc
     4441 atgagcacag ttattactgg tatcgagatg ttcagaaagc agcttgatga agcttatgct
     4501 ggtgacaaca tcggtgctct cttgagaggt atcacaagag aagatatcga gcgtggacaa
     4561 gttatagcca agcctggtac aatcaagtca tacacaaagt ttaagggcca ggtttacgtt
     4621 cttactcaga acgagggtgg aagacacaag cctttcttca gcaactacag acctcagttc
     4681 tacttcagaa ctgacgttac cggtattatc cagcttccag aaggcgtaga catgtgcttg
     4741 cctggcgacc acgtagaaat tactgttgaa cttattactc caatcgctat ggaagaaggt
     4801 ctccgcttcg ctattcgtga aggtggaaag actgttggtt ccggtacagt tacagccatt
     4861 atcgactaat tggaatcaga tgatttttgg cggacggtgc aaactgtctg ccttttttta
     4921 ttcccagatt ctataaataa aattggaaaa aaacaaaagt attaaaatcg ccaatattaa
     4981 gaataatatg aaagaaagag tttatctttt tcaatagagt ttgggaggat atcaaatgaa
     5041 agcaaaatat atcttagtat tcttacttgc aattatgatg atattttcat ttgctgcatg
     5101 tagaaatgga ggactgttgg atccatcacc aggtataaca atcagtccta acgtgactga
     5161 tgatggtgat ggtgacatga gtcctgacct gagcccagac ttgagtcctg accttagtcc
     5221 tgatgtaagt ccgggtgcag gaggcagtcc aggtgccgga ggtccaggta catctccgga
     5281 aggctcacct ggtggtaacg gtggagccaa tggtgcctcg aaacaagtct agtcaggttg
     5341 tgaatggtat atatagatac aggccttcga tctttttcag gtcgaaggtt tctttttata
     5401 cggtttttgt attaaaatat attcacaggg tttgccataa aaaagacaca gaaatgatgt
     5461 agtatagcta agaggtgttg tttaatgatt aaagtgctta tatgcgatga caatagacag
     5521 attacgtcca tattggaaga gtattgcaag agagaagggt ataaagtgaa agttgcttat
     5581 gatggcgaag aggctcttga gttcttttca tcctgtatgc ccgatattat tcttcttgat
     5641 gtgatgatgc caaagcttga cggcttttat gtttgcaagg aaatcagaaa gaagtccaat
     5701 gttccagtta tcatggttac agcaagaggg gaagattttg aaaagataat gggtcttgaa
     5761 ataggggcgg atgactatat tgtaaagcct ttttctggtg gagaagtaat ggcgagaata
     5821 agggcgatat taagaagatg ctcttatgaa ggaagaacgg aaggccatga attgaaaatt
     5881 gcaaaccttt acataaatat ggatagctat gaggtcatga ttgacgatga agaggtgtct
     5941 ttgactaaaa aggagaccga gatactttgg cttcttgcat ctaacagcgg aaaagtcttt
     6001 tcaagagaga atcttcttga caagctctgg ggttacgatt attacggaga catgaggact
     6061 gtggattctc acataaagag gctgagagca aaaattgatg cgtttccgca tgaatcgtgg
     6121 gatgttacca cgatatgggg agttggatac agatttaccg aatacaagga aggcaaggga
     6181 taaacatgcg aaaaaagctt gtcttcaaac taactttcta ctttacaatt gtgcttttaa
     6241 tactgtcaat aggtataggg attgtgttta caatcctttt tagaaaccat actgttgacc
     6301 tatacaaaag aaatcttgta aacagcgcag taggtgtttc agagacacta tcggaattca
     6361 ctttcagaat gcagtattca agatttccct tgtttgactt tccaaacaga aacaactttt
     6421 atttctccaa tgatgcccaa gtgtatataa gttttttgaa cagtatcaca atggcgaatg
     6481 tatggataat tgacagggac tacaacttta tcactacagg cacatcatat tacgatggaa
     6541 gccgagtgta cacattcagc gagctgccgc ataatgcgga gaaagtaata caaaaggtgt
     6601 tcgaaggtga aaccaccttc agcgaagtgt ttggcaactt cttcgaaaca ccaaccctca
     6661 ccgtgggtgt tcctattgtt tctcagaatg gagaagtaat tggtgttgtt ctgcttcaca
     6721 gtcccataga ttatatgcag aaggcgatag agcagggtat caagacgctg gttataagta
     6781 tactgctggc actagttttg tctgtgattc tgtctttttc ctttgcctgg gcttttacaa
     6841 aaccaatgga caagatacgg aatgttacac tggaactggc aaaagggaat tatgaggtta
     6901 aaacggatgt atcccaggat gatgaaatag gggatgttgc aaaggctgta gatatattaa
     6961 gtgaaaggct taatgaggca agcagggaaa gagaaaagct tgacaagatg aggaaggatt
     7021 ttgtagccaa catatcacat gaacttagga cccctgttac agtgataaga ggttcggttg
     7081 aagcgttgca tgacaaggtg gttacggaac caaagcaggt ggaagaatac tactctcat
//
LOCUS       DAONVY010000042         7138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7947_length_7138_cov_2.818580, whole genome shotgun sequence.
ACCESSION   DAONVY010000042 DAONVY010000000
VERSION     DAONVY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_7138_cov_2.818580"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLI11_02005"
     CDS             complement(<1..999)
                     /locus_tag="PLI11_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51668.1"
                     /translation="MLKTKKENTEENSEKQPFGLQFMQSCKELNNIVKKYYKKLLPWI
                     ATYFAIAIVSLLMTEYGNNMLSSSFPKTLLLLFPRLFFLFVLTLTSFISDKKYVYFSL
                     LSGVFLLWTGILAKVSPAIYISLFIICAGFALYFHYRNKRNGMEHDPYKAIFKRIHFW
                     TFSFLLLTLAIELVHQFNPIRAIPRIVTNPDMFMLNVAIVMGIGMFVFICKKRNFAFA
                     MYAAFWVILSYISYLKYSNVNEPVLLLDVFQINEGVAAAFKVLDWIDLALIVVILVGL
                     VIGIRAFSKKAKKVAFDMKNLVIILLLYALLPGPIIGAGSVGSMKTHERFLKDIFFH"
     gene            1139..2824
                     /locus_tag="PLI11_02010"
     CDS             1139..2824
                     /locus_tag="PLI11_02010"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPZ51669.1"
                     /translation="MILAINGKTEYRIEGEYEFAIKEFCDLFNKLTGAVLERAQEDSD
                     KLIKLGGVKPKDKGEDAYVIRTEGDNLILSGNTNRGILYAIYTFFERYAGCRWFTSDC
                     EYVPRKDEFTIPEINIDESPFFWYRETFYKDAFEPSFALRNKLNGHHMQLEREHGGKF
                     EYGLGFVHTFHTIIPQEEYFKTHPEYFPMIDGERTTGPHHQICLSNPDVLKITIEKVK
                     KAFDENPNVKIVSVSQNDTFEEGNQCRCDQCSAIEEEEGSPAANVIRFVNSVADAIKD
                     EYPDKYIDTLAYRYTRKAPLKTKPRDNVIVRLCTIECCFSHPITTCDQVNDKGVHRNG
                     SFIEDIVAWSKIANNIFIWDYIVNFLNYLTPYPNLYALQKNMQFFAEHNVKGVFSQGC
                     GEIKFSEYAHLRAYVIAKLLEDPYCDVNLIIDEFMYGYYQNAAAPLRKYIDVLNKKAE
                     EENFHFGLYDDPNRDFLTKEFVDYADSLFDMAERMADDETILYRVRRTRMSLRYAKLF
                     LMDGSLEERRRELEKFFEDLRYFKIESLLERGNLDSSYERMRQIVTPEDVERK"
     gene            2827..4041
                     /gene="pepT"
                     /locus_tag="PLI11_02015"
     CDS             2827..4041
                     /gene="pepT"
                     /locus_tag="PLI11_02015"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPZ51670.1"
                     /translation="MKAYERFLEYVKINTQADPKSGSVPSTEGQTVLAKMLVEELKSL
                     GVENAYLDDSGVVYGKIKGSANKACKTVGFIAHLDTSPDMSGENVRSRIVKNYDGGRI
                     ELSDHAHMDPSTFPNLENKVGHDLIVTDGTTLLGADNKAGIAIIMSFVERIMKGDILH
                     GDISIAFTPDEEIGRGADNFDVEAFGADIAYTVDGGDISVFEYENFNAASANVVVNGS
                     SIHPGYAKGKMKNAILLAMEFQSLLPAFMNPAYTEKYEGFYHITDINGMCEKAEMEYI
                     IRNHDDKLFNEQKEFFMKAAEFMNAKYGENTFEVSVKDSYYNMRKMIEKDMTVVDIAL
                     EAMREIGITPQIEPIRGGTDGARLTYMGLPCPNIGTGGYNFHGKYEYLSINEMDKSVE
                     LITRITGKLISN"
     gene            complement(4028..4609)
                     /locus_tag="PLI11_02020"
     CDS             complement(4028..4609)
                     /locus_tag="PLI11_02020"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPZ51671.1"
                     /translation="MKEYNISKNAWLDIDIYPWGCDYRPKTQAKIQKGSNGFEITMKS
                     YEKNIRMEKTERNSNVHTDSCMEFFFNAEPEKSKTYMNFEINPKGIMYIGFSKTGDRS
                     GTGPIEAPDNEYFNMKSRITDEYWEISYTVPYEFIKQYFPTFDENKTEYIKANFYKCG
                     DLTKHPHFGVWNNIETEEPDFHRPEFFGVINLR"
     gene            4745..5032
                     /locus_tag="PLI11_02025"
     CDS             4745..5032
                     /locus_tag="PLI11_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HPZ51672.1"
                     /translation="MAEIRYKIIKRLGVINSSNNGWAKEVNIVSWNDGAAKLDIRDWE
                     QSSNKMRKGITLFRDEAEKLREILNSMDMDDLEQRYSKDEFALLDEYVDVR"
     gene            5180..>5836
                     /locus_tag="PLI11_02030"
     CDS             5180..>5836
                     /locus_tag="PLI11_02030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HPZ51673.1"
                     /translation="MYEYYNNDYRSRKKSITLSVKSMIAVCILCGFIGGGMSLVGFNV
                     FNKDVDSGNAFLGATETHDKSQSQNTKNITNISVESVTSYANAVAKKVLPSIVGINVT
                     TKVVNKGWFGDTVTEKVGEGSGIIYKEDGYIITNYHVVQDSIITAGEMNPDSQIKVHL
                     YEDPETGIDARVIGYDIASDLAVIKIEKDNLTAIEIGDSDKVEVGDIVLALGNPGGLK
                     F"
     assembly_gap    5837..5846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5847..6408
                     /locus_tag="PLI11_02035"
     CDS             <5847..6408
                     /locus_tag="PLI11_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPZ51674.1"
                     /translation="GSVSQGIISGLNRSVEVDGVYKDLTLIQTDAAINPGNSGGALVD
                     VEGRLIGVNSVKLAATGYEGMGFAIPVNDVVRIIESLIENGNAVYTYLGITEDTRYTA
                     ELLETNGYPGGVVVGSVANGSPAEKAGIKTYDIIVEFNGIKVRTMQELQNIKSSLRKG
                     DVVNIKVFRQTSLFRGDYITFDVTLA"
     gene            6460..>7138
                     /locus_tag="PLI11_02040"
     CDS             6460..>7138
                     /locus_tag="PLI11_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPZ51675.1"
                     /translation="MQITFVQEIKDLMVLDKERIALESHIDIDDEENFEELCKLVDEA
                     YEVAVPKVCITKMKVRMGEKDDEVYLNDILFKSRILAANINESVDTYGIVCTCGVEIE
                     KWAQKYRNDPLLAYFADAIMVEVLGNSVVYAMKYLSSLSDMSISHASPGSTRDFPLSN
                     QVKLFEAIGNVTEKTGVYLTPEYLMKPLKSVSGVYFANDSDYSDCKLCTRMDCPRRRE
                     PYDDSMFS"
BASE COUNT         2303 a         1141 c         1629 g         2055 t
ORIGIN      
        1 atgaaagaaa atgtctttta agaatctttc atgggttttc atagaaccaa cactaccagc
       61 acctataatg ggtcctggaa gaagtgcata caaaagaagt atgatcacca ggtttttcat
      121 gtcaaaagca actttctttg cttttttgct gaatgccctt attcctatga caagaccaac
      181 gagtatcact acaattagcg ccagatctat ccagtcaagt accttgaaag cagcggcaac
      241 accctcgttg atttggaata cgtccagtaa aagaacaggt tcgtttacat tggaatactt
      301 cagataactt atatatgata gaataaccca gaatgccgca tacatggcaa aagcaaagtt
      361 tctttttttg caaataaaga caaacatacc tatgcccatt actattgcaa cattgagcat
      421 gaacatgtcc ggatttgtaa ctattctggg gattgccctt attggattaa actggtggac
      481 aagctcaatt gccagtgtta ggagcaaaaa tgaaaatgtc caaaaatgta ttcttttgaa
      541 aatggctttg tatggatcat gttccatgcc atttctttta tttcgataat gaaagtacag
      601 cgcaaagcct gcgcatatga taaataagga tatatatatt gctggggaaa cttttgcaag
      661 aattcccgtc cataatagaa aaaccccgct caacagggag aagtatacgt atttcttgtc
      721 ggatatgaaa cttgtcagtg taagtacaaa caaaaagaat aaccttggaa acaaaagtaa
      781 tagagttttg gggaaactgg aacttaacat attgtttccg tattctgtca ttaatagtga
      841 aactatggca atagcaaaat atgtagctat ccaaggtaat agcttcttgt aatatttttt
      901 tacaatatta tttaattcct tgcaggattg cataaactgc aatccaaaag gttgcttttc
      961 actgttttct tctgtatttt ctttcttagt ctttaacata ataccttgaa tgacctttcc
     1021 tgttgtttat atattttttc aaaccttaaa tgattttagc acaaaatgcg tcttgtatca
     1081 attttttctt tctcatggta taatgtaggc aaaagtcaat cattggaggt aatttatcat
     1141 gattcttgca ataaacggaa aaaccgagta tagaatagaa ggagaatatg aatttgcaat
     1201 aaaggaattc tgtgacctgt ttaataagct cactggtgct gttttggaaa gagctcagga
     1261 ggatagcgat aaattgatta aactgggcgg agtgaagccc aaggacaagg gtgaggatgc
     1321 atatgtgata agaacggaag gtgacaatct gattctgtca ggcaatacaa atagaggtat
     1381 tctgtatgcg atatacacct ttttcgaaag gtacgcaggt tgcagatggt ttacatccga
     1441 ttgtgagtat gtgccaagga aagatgaatt tacaatacct gaaatcaata ttgatgaaag
     1501 tccattcttc tggtacaggg aaaccttcta caaggatgcc ttcgaaccat cctttgcgct
     1561 tcgaaacaag ttgaatggac accacatgca gttggaaagg gaacatggag gaaagttcga
     1621 atacgggctt ggttttgtgc atacttttca taccatcatt ccacaagagg aatatttcaa
     1681 gacccatcca gaatatttcc ctatgattga tggagaaaga accacaggac cccatcacca
     1741 gatttgtctt tccaatcctg atgtattaaa gattacaata gagaaggtta agaaggcttt
     1801 tgatgaaaat ccaaatgtga agattgtatc agtttcgcag aatgatacct ttgaggaagg
     1861 aaaccagtgc cgatgcgacc agtgcagtgc aatagaggag gaagaaggct ctcctgcggc
     1921 taatgttatc cgttttgtaa acagtgtggc tgatgcaata aaggacgaat accctgacaa
     1981 atacatcgat acattagcct acaggtatac aagaaaagcg cctctgaaaa caaaaccgcg
     2041 tgataatgtt atagtaaggc tgtgtacaat agagtgctgt ttcagccatc ccattactac
     2101 atgcgatcag gttaacgata agggtgtgca cagaaatggc tcctttattg aagatattgt
     2161 ggcatggagc aaaatagcca acaatatctt tatctgggac tatattgtca acttcctaaa
     2221 ctatcttact ccttatccga atctgtatgc ccttcagaag aacatgcagt tctttgctga
     2281 gcacaatgtc aaaggagtgt tttcacaagg ttgtggagag attaaattca gtgagtatgc
     2341 tcatctgcgt gcatatgtaa ttgcaaaact tttggaagat ccgtattgcg atgttaactt
     2401 gataattgat gagttcatgt atggatatta ccaaaacgca gcagctcctt tgagaaagta
     2461 tatagatgta ttgaacaaga aggctgaaga ggaaaacttt cattttggat tatacgacga
     2521 tccgaacagg gattttctta ccaaggaatt tgtagattat gcggacagcc tttttgatat
     2581 ggctgaaaga atggcggatg atgagacaat actctacagg gtaagaagaa cccgcatgtc
     2641 attaagatat gcaaaactgt tcctaatgga tggcagtctt gaagaaagaa gaagggaact
     2701 tgagaagttc tttgaggatt tgagatattt caaaatagaa tcccttctgg aaagaggaaa
     2761 tttggattcc agttatgaga gaatgaggca aattgtgaca cctgaggatg tggagaggaa
     2821 ataggaatga aagcgtatga aagattttta gagtatgtga agataaatac ccaggcggac
     2881 cccaaaagcg gaagtgtgcc cagtacagaa ggacagacgg ttcttgcgaa aatgttggtg
     2941 gaagagctca aatctctggg tgttgaaaac gcatatcttg atgattcggg tgtagtgtat
     3001 gggaagataa aggggagtgc gaacaaggcg tgcaaaactg taggtttcat agctcatctt
     3061 gacacgtccc cggatatgtc cggagaaaat gtcagatcaa ggattgtcaa gaattacgat
     3121 ggcggtagaa tagaattaag tgaccatgca cacatggatc cgtctacatt tccgaacttg
     3181 gagaacaagg ttggacatga tttgatagta acggatggaa cgacactgct tggtgccgac
     3241 aacaaggcag gcattgcgat tataatgagt tttgttgaga gaatcatgaa gggggatatt
     3301 ctgcatggtg atatttccat tgcttttacc cccgatgagg aaattggaag aggtgcggac
     3361 aactttgacg ttgaagcttt tggtgcagat attgcgtata ctgtagatgg tggggacata
     3421 agcgtgtttg aatacgaaaa cttcaatgca gccagtgcaa atgtggttgt aaatggaagc
     3481 agtattcacc cgggttatgc aaagggtaaa atgaagaatg ccattttgct tgcaatggag
     3541 tttcaaagtc ttctgcctgc gttcatgaac ccagcatata cggaaaaata tgaaggtttt
     3601 tatcatatta ccgacattaa tggtatgtgt gaaaaagctg aaatggagta tattatcaga
     3661 aatcatgatg acaagttgtt taacgaacaa aaagagtttt tcatgaaagc cgctgagttc
     3721 atgaatgcca aatatggaga gaataccttc gaagtttcag taaaggattc atattacaac
     3781 atgcgcaaga tgatagaaaa ggacatgaca gttgtagata ttgcattgga agctatgagg
     3841 gaaataggaa taaccccaca aattgaacca attcgtgggg gaactgacgg tgcaaggttg
     3901 acgtatatgg ggcttccgtg tcccaatatc ggtacagggg gctacaattt ccacggcaaa
     3961 tatgagtatt tgtccataaa cgagatggac aagtccgtag aattgattac cagaataact
     4021 ggcaaactta tctcaaatta attactccaa agaattcggg cctgtgaaaa tcaggctctt
     4081 ctgtttctat attgttccac actccaaagt ggggatgttt tgtcaaatct ccgcatttat
     4141 aaaagtttgc ttttatatat tccgtcttgt tttcatcaaa ggttggaaag tactgcttta
     4201 taaactcata cggtacagta tagcttatct cccaatactc atcagttatt cgtgacttca
     4261 tgttgaaata ttcattgtcg ggagcttcaa tggggcctgt tccgcttctg tctcctgttt
     4321 tgctgaatcc aatgtacatg attccttttg gattaatctc aaagttcatg tatgtttttg
     4381 atttttcagg ttctgcattg aagaagaatt ccatgcatga atcagtatga acattcgaat
     4441 tcctttcagt cttctccatc ctaatattct tctcatacga tttcatggta atttcaaaac
     4501 catttgatcc tttttgaatt ttggcttgtg ttttcggcct gtagtcgcaa ccccatggat
     4561 atatgtctat gtccagccat gcattttttg agatattata ttccttcatg ccatcacctt
     4621 caatttatgt tttgagtaat tatatcatgg aaagaaaaaa acaacactgt tttattgccc
     4681 aggttatgaa aatcagcaaa tatattagta taattgggat gtaaagttaa caggaggtga
     4741 aaagatggcg gaaatcaggt ataagattat taaaaggctg ggagtaatca atagctcaaa
     4801 taacggatgg gcaaaagaag ttaatattgt cagctggaat gatggtgctg ccaagcttga
     4861 catacgcgac tgggagcaat catcaaataa aatgaggaag ggcataacat tatttaggga
     4921 cgaggctgaa aaactcagag agatattgaa cagtatggac atggatgatt tggagcaaag
     4981 atattcaaaa gatgagtttg cgctgctgga tgaatatgtt gatgtaagat aagtttgacg
     5041 gtttaaccgt caaatttttt tggcatttat ctctttcttt tggtgataat atgatagatg
     5101 tttttgtcac ataaaaaaca aagaattgta aatataatgt caaaaatatc aggtaacata
     5161 tactcaagag gtgatgccaa tgtatgaata ttacaataat gattatagaa gcaggaaaaa
     5221 gagtataaca ttgagtgtta aatcaatgat tgctgtttgc atattatgtg gattcatagg
     5281 tggaggcatg tccttagtcg ggtttaatgt gtttaacaaa gatgttgaca gcggaaatgc
     5341 ttttttgggt gcgacggaga cacatgacaa aagtcagtcg cagaacacaa agaacattac
     5401 gaatatttct gttgagagtg taacatccta tgccaatgca gttgcaaaga aggttttgcc
     5461 ttcgattgtc ggtattaatg taacaaccaa ggtagtaaac aagggatggt ttggagatac
     5521 cgttactgaa aaagtgggag aaggcagcgg tatcatatac aaggaagatg ggtatataat
     5581 tacaaactat catgtggtac aggactccat tataacagcc ggagaaatga atcctgactc
     5641 ccagataaag gtgcatctgt atgaagaccc tgaaacagga attgatgcta gagtcattgg
     5701 gtatgatatt gcatcggatc tggcggttat aaaaatcgaa aaggacaatc tgactgctat
     5761 agaaattgga gattctgata aagttgaggt aggagacata gttcttgcct tgggaaatcc
     5821 cggtggcctt aaatttnnnn nnnnnntggt tccgtgagtc aggggataat tagcggtctt
     5881 aacagaagcg tagaagttga tggtgtttac aaagacctta ctctgataca aacggacgct
     5941 gcaataaatc caggcaattc cggtggagct cttgtggatg ttgaaggaag gttgattggt
     6001 gtaaacagtg taaaacttgc cgcaacagga tatgaaggaa tggggtttgc catacctgtg
     6061 aatgatgttg tcaggataat cgagagcttg attgaaaacg gaaatgcagt ttacacatac
     6121 cttggaatta ctgaggatac aaggtatact gcagagcttc ttgagacgaa cggttatcct
     6181 ggaggggttg tcgtaggtag cgtggctaat ggaagtcctg ctgaaaaggc gggcataaag
     6241 acttacgaca taattgttga attcaatggt atcaaggtaa ggaccatgca ggagcttcag
     6301 aatataaaaa gcagtttgag aaaaggcgat gtggtgaata ttaaggtgtt cagacaaaca
     6361 tcgttgttca gaggagacta tatcactttc gatgtgactc ttgcatagga cttttgcctc
     6421 ctgtataatg aacttaagta caacatttgg aggtaaaaga tgcaaataac gtttgttcaa
     6481 gagataaagg atttaatggt gctggataaa gaaagaatag cactggaatc acatattgat
     6541 attgatgatg aggagaactt tgaagaactt tgcaaacttg ttgatgaagc ttatgaagtg
     6601 gcagtgccaa aagtatgcat tacaaagatg aaggtgagga tgggagaaaa ggatgacgag
     6661 gtgtatttga atgacatatt gttcaaatca aggattctgg ccgccaacat taacgaaagt
     6721 gtggatacat acggaattgt ctgtacttgt ggagttgaaa tagagaaatg ggcacagaaa
     6781 tacagaaatg accctcttct tgcgtatttt gccgatgcta ttatggtgga ggtgcttgga
     6841 aacagtgttg tttatgcaat gaaatatcta agctcgttga gcgatatgag catatcacat
     6901 gcaagtccgg gctcaacaag ggattttcct ctttcaaacc aggtgaaact gtttgaggca
     6961 attggcaatg ttacagaaaa aaccggagtg tatctgactc cggaatatct gatgaagcct
     7021 ctgaaaagcg tttcgggcgt atactttgca aacgattcgg attattccga ttgcaagctg
     7081 tgcacaagga tggattgccc aagaagaaga gagccttatg acgacagcat gttcagta
//
LOCUS       DAONVY010000043         7116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_7974_length_7116_cov_2.953123, whole genome shotgun sequence.
ACCESSION   DAONVY010000043 DAONVY010000000
VERSION     DAONVY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7974_length_7116_cov_2.953123"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLI11_02045"
     CDS             complement(<1..405)
                     /locus_tag="PLI11_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51676.1"
                     /translation="MKNSNFLRIYTEIVNEKLPENNLECILNALWPAGRDFDNSLKLG
                     ICISGSGVLYSDYGSQNFREDNAMIIFPNQKYKLYAYEPHTKWVCMETDVYRLFGESS
                     LINAEYAYKLLKESYDNSIIISGSKYNHIKDII"
     gene            complement(430..1431)
                     /locus_tag="PLI11_02050"
     CDS             complement(430..1431)
                     /locus_tag="PLI11_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPZ51677.1"
                     /translation="MTPQELHMAIARDRVYPLSFNELNDFNTQKEKIKKTLYGLLGMP
                     QKTTNPKPVIEFEKTDHEDYDEIRFHVESEPGFFVPAHLILPKERGVKLPTVICLQGH
                     SSGMHRSLGRVKYTNDVEGDVGDRAFALQAVRRGYACVIMEQRGFGELKGIEQDGGTN
                     CLHMSLQAIMLGRTLIGERCFDVSRLIDALEYFEEVDLNRLGIMGNSGGGTTSFYAAC
                     VDPRIKVCMPSCAFCPYYDSIISIHHCSCNYIPQMLEYMEMHDLSILIAPRPLIIVAG
                     KDDEIFPIEGVKRGFETVKKIYKAAGAEDNCQLIIGDGGHRFYADVSWPVFDRYIIY"
     gene            complement(1984..2763)
                     /locus_tag="PLI11_02055"
     CDS             complement(1984..2763)
                     /locus_tag="PLI11_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51678.1"
                     /translation="MNIYWNNELLLSIEMSDIEYITYEGKGSAYFYTKAVVRNSSGTV
                     LSTINDARIAAKEDATIAFAVRGTQMYVDNVYLDTNYETSAGASENAAVYSGSQIRTA
                     LVPYRDKEGNIDLDDMRQGLRFCFDILEIDTVFEHDGKSYTVKNTGALVALKDKLEDE
                     NLMKVSNAGRVPGLLIQDYLIDVVIDDDEYKSAYIYNIPEANKDTLICARAYLECVSE
                     SGDIVYFYTPVQERSLTMVYERIVTEGDIDTLDEAVKAWWR"
     gene            complement(2845..4125)
                     /locus_tag="PLI11_02060"
     CDS             complement(2845..4125)
                     /locus_tag="PLI11_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51679.1"
                     /translation="MVTLVPSNVFVAANNRYFSEDFEGSEVDLSENWLERSAYGTSEG
                     DWDDRIVTDPADSGNKVYRMLWSRNSPQLLKWNKPLTSYHLSADVKGGGSYPKFEGQR
                     AHPASIYLRTTLHNLGRPEYQSESPDLESRVGQFGIVITLRDSWISVDIKKSDNAHYD
                     LNNNPGTGNWIYFKNIGNNCNANFTNISVTDDGHLVKIYWNHSLLLAIELYDKELVEY
                     EGKGSGWFYTRAVVRNGSGSVISTVTDTRIAANYDATVAFAARGTELYIDNLSLSENF
                     RYPEYTEDFSDLTYEYLWNDWTEKCAHGNSLSELDLRIVEDPRDSSNMVYKLHFYTLG
                     SSLLKWKHRLNKYEFSADLKGGGYYPDFQGTRHNPASVYLRTTHANLWVPEYERYGDE
                     NVVGYAGVVFTIRDSYVSIDIKNSQNSYSNEGES"
     gene            complement(4353..6956)
                     /locus_tag="PLI11_02065"
     CDS             complement(4353..6956)
                     /locus_tag="PLI11_02065"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPZ51680.1"
                     /translation="MVRRIHDCLNAKEDRSYIFPFLWLHGEEHDRLVEEIDAVYKSGI
                     REICFESRPYADFCGDKWWEDFAFMLEQARHRDMRVWLLDDKHFPSGYANGAIEKKYP
                     HLRRKCVRIECLDVAGPVIGSAFIADRYDKNTEELISVSAYRRTGKDEEVDGSTGVLL
                     TNNVQNGLVYWDVPEGVWRVFFVIKTQRGPAHFKNYIDMCDEESCKVMIKEIYEPHYE
                     RFGKYFGNTFRGFFSDEPCFANNIGSYYDTLGIPSLILPWRNDMIEMLAERANLSAEE
                     AELLLPCLWYTVAGKTSQLRYAYMETVSHLYRDNFSRMVGNWCRERGVLYIGHVIEDM
                     NTHMRLGYGAGHFFRAQDGQDMSGIDVVLHQILPGITDMLNKSSSSRGVADPEFFINT
                     LPKLGASCAHLDPRKKNRAMVELYGAYGFAEGLPVMKYLTDLMLAGGINHFVPHAFTA
                     KDNDPDCPPHFYANGRNPQFPLFRLLMEYLQRASHVLYGGVHVANAAVFYNAEGEWCG
                     GKYRLFQDVATNLAKEQIDFDIVSIDALVNATVKDKKLIVNEEKFDALVISYSEVLPK
                     KLLDICASFSSEGLSVVFTEDYPDRTEKNFESEEELNAIREHTRKLVENCIVVPTDKV
                     AAFFREKGFVDVELKDTNPFTRVLHLSEDDGDAYFFFNLSMEKDVDTKVFFKNNRFEN
                     FYEYDIWNNKVYKCQVDKDGIRLVLPKGHATLLFACSDEFENAPTKQYDFDFKKLNIT
                     FDVYLIEHNSKTYYGKMESDALKNLAFEKSGFCGVIRYEGKIETDAKVKFLNLGIVGE
                     IAQLYVNDTFCGTCISHPYIFDVSNTWETGENSIVIEVTTNPGYMIRDNFSRMLPLPP
                     MGLLGPVEISR"
BASE COUNT         2117 a         1696 c         1080 g         2223 t
ORIGIN      
        1 tattatatcc ttaatatggt tgtacttact accgcttata attattgaat tatcgtagct
       61 ttccttcaga agcttgtatg catactcagc attaatcaag gaagattctc caaaaagcct
      121 gtaaacatct gtttccatgc atacccattt ggtatgaggt tcatatgcat aaagcttata
      181 cttttgattt ggaaatataa tcattgcatt gtcttctctg aaattttggc taccataatc
      241 actgtataaa acaccactgc cggaaataca gattcccagc ttcaagctgt tatcgaaatc
      301 ccttccggca ggccacaatg catttagaat acattctaag ttattctcag gtaatttttc
      361 atttactatc tccgtatata ttctaagaaa attggaattc ttcatatata caaaaccccg
      421 ataagtattt taatatataa tatatctatc gaaaacaggc catgatacat ccgcataaaa
      481 cctgtgtccc ccatccccaa taataagctg acaattatct tccgctcctg ccgccttgta
      541 tatttttttg acagtttcga accctctttt tacaccttca ataggaaaaa tctcatcatc
      601 cttaccagca acaattatta atggccttgg agcaatcaaa atcgaaagat cgtgcatttc
      661 catatactca agcatttggg ggatatagtt acacgaacaa tgatgaatgc ttattattga
      721 atcataatac gggcaaaacg cacaagaagg catgcacacc ttgattcttg gatcgacaca
      781 agctgcataa aacgacgttg tcccaccacc tgaattgccc ataataccga gtctgtttag
      841 atcaacctcc tcgaaatatt caagagcatc tattagcctt gaaacatcaa agcacctttc
      901 acctataaga gttctaccaa gcattattgc ctgcagagac atatgcaggc agttggttcc
      961 accatcctgc tcaattcctt ttaactcacc aaaacctctt tgttccataa ttacacaggc
     1021 ataaccacgt cttaccgcct gcagagcaaa tgccctgtcg ccaacatcgc cctctacatc
     1081 attcgtgtat tttacccttc ccaaagatct gtgcattcct gaagaatgtc cttgcaaaca
     1141 tattactgtt ggaagtttta cgcccctttc tttaggaaga atcagatgag ctggcacaaa
     1201 gaagccaggt tcgctctcaa catgaaatct aatttcatca taatcttcat gatcagtctt
     1261 ctcaaattca ataactggct taggattggt agttttttgg ggcatcccta aaagaccata
     1321 aagtgttttt ttgattttct ccttctgggt attaaaatca tttaattcat taaaggataa
     1381 gggataaacc ctatccctgg caatagccat atgcaattcc tgcggtgtca ttttttcact
     1441 ccttttattg tttgaatata taattcaacc taccatatgg cgctgaatca ccattttaat
     1501 tggtattttt accatcaact cattgtcttc gattttgcaa tccaacaaac ccactcttgt
     1561 ccacttatta cccactacaa tattatccac tgtttttaca taaaagtaaa tactctcgcg
     1621 ttactctgca accttaagag tttcaaaacc atttacttcc atccagtatc tccaaaagta
     1681 tattctccac tctcaaaatc ttcccgtata cgtctcatat tgaatttctt ctgcccgatt
     1741 taacgagcag aagaataatg ataaatacag aataaaacgt tttacaaaag tcggaaatac
     1801 aacaatttgc aaaagcatgc tattgttcca ataaattgcc aacgtctata acaattatat
     1861 tcttaaatca aatcctcaaa atcaatacca atatattcac tcaaagtaat attttctatc
     1921 gtttcaaaat atatgatttc cagttcaggt tccacataca cgcttatcat attgaaaacc
     1981 tccttatctc caccatgctt ttacagcctc gtccaaagta tcaatatccc cttcagtgac
     2041 aattctttca taaaccattg tcaatgatct ttcctgaaca ggagtataga aatacactat
     2101 atcgccactt tcggatacac actcaagata agctcttgca catatcaaag tgtccttgtt
     2161 tgcttcagga atattgtaga tgtatgccga tttatactca tcgtcatcta taacaacatc
     2221 tataagataa tcctgaatga gtaaaccagg cactctgcca gcatttgaaa ctttcattaa
     2281 attttcatct tccagcttgt cttttaatgc gaccaatgca cctgtgtttt ttacagtata
     2341 gcttttgcca tcatgttcaa agacagtatc aatttccaat atatcaaaac agaatctaag
     2401 accctgcctc atatcatcta aatcaatatt gccctcctta tccctatacg gaaccaaggc
     2461 tgtcctaatt tgagaaccgg aataaacagc agcattttca gaagcacctg ctgaagtttc
     2521 ataattcgta tccaagtaaa cattgtctac atacatctgc gtgccacgca cggcgaatgc
     2581 aatggtagca tcctcttttg cagcaatacg tgcatcattt attgtactga gtaccgtacc
     2641 agaactgttt ctgacaactg ccttggtata aaaatacgca ctcccttttc cttcatatgt
     2701 aatatactct atgtcagaca tttcgatgga cagcaataat tcattgttcc agtatatgtt
     2761 cattacatta ccatcgtcgg ttacatgaag atttgtaaag ttagtattgc agttatttcc
     2821 tttgcctgta aagtaaaacc agtttcagct ttcaccctcg ttagaatatg agttttgtga
     2881 gttctttata tctattgata cgtaagaatc gcgtatcgtg aacaccacac cagcataacc
     2941 tacaacattt tcatcaccat acctctcata ctcaggaacc cagagattgg catgtgttgt
     3001 tcttaaatac actgatgcag gattatgcct cgtaccttgg aaatcaggat agtagccacc
     3061 accctttagg tctgctgaaa actcatactt gttcaatctg tgtttccatt ttaacaaact
     3121 ggaacccaag gtatagaaat gaagtttgta caccatgttt gagctatccc gtgggtcctc
     3181 cactatcctg agatcaagct cagataaaga gttgccatgt gcacatttct cagtccagtc
     3241 gttccataaa tattcgtatg tcagatcaga aaaatcttcc gtatactcag gatatctgaa
     3301 attttcactc agggataggt tgtctatata caattctgta cctcttgcag caaacgcaac
     3361 cgtagcatca taattggcag ctattctcgt atcggttaca gttgatatta ctgaaccgga
     3421 gccatttctt actacagctc ttgtatagaa ccagccgcta cctttacctt cgtattcaac
     3481 caattccttg tcatagagtt ctatggccaa aagaagtgag tgattccaat atattttaac
     3541 aagatgccca tcatcggtaa cactgatatt tgtaaaatta gcgttgcagt tattaccaat
     3601 atttttaaaa taaatccaat ttcctgttcc tgggttgttg ttaagatcat aatgtgcgtt
     3661 atcagatttc tttatatcaa cagaaatcca tgaatccctt aacgtaataa caatcccaaa
     3721 ctgaccaact cttgattcta agtctggcga ctcgctctga tactcaggtc tacccagatt
     3781 atgtagagtt gttcgcaaat atatggatgc gggatgtgca cgttgtcctt caaattttgg
     3841 ataactacca ccgcccttta catccgccga taaatggtag gatgtaagtg gtttgttcca
     3901 tttcagcaat tgaggactat ttctgctcca caacatcctg tatactttgt tgccgctgtc
     3961 tgcaggatcg gtaacaatac gatcgtccca atcaccttcg gaagtgccat aagcacttct
     4021 ctccaaccag ttctctgaca aatccacttc gctgccttca aagtcctccg aaaaataccg
     4081 gttgttggct gccacaaaga catttgaagg tacaagcgtc accaaggtaa aacaaaagac
     4141 tcagcaggat tgctcttctc atatcgatca cctcataaca atatttcgaa gtttcgctat
     4201 acatacataa ttctattata tttccacatt caataaacga acaacataaa ttcgctcaaa
     4261 tatttattta tatataataa attcgctctt aacactgcac aaaaagtcct ctcagaaaat
     4321 caatccgaaa ggactttatt tgtgcatata cactatctag aaatctctac cggccctagc
     4381 aaacccatcg gtggcagggg aagcatcctt gaaaagttat cccttatcat atagccggga
     4441 ttggttgtaa cctcaataac aatagaattc tcacctgttt cccacgtgtt tgacacatcg
     4501 aaaatatatg ggtgtgatat acatgtccca cagaacgtgt cgtttacata caactgtgct
     4561 atttccccaa caatacccag attaagaaac ttaactttag catcagtttc aattttacct
     4621 tcataacgaa tcacaccaca aaacccactc ttttcaaatg ccagattttt aagtgcatct
     4681 gattccatct tgccgtaata cgttttactg ttgtgttcta ttaaataaac atcaaaagtg
     4741 atatttaatt tcttgaaatc aaagtcatat tgtttggttg gtgcattctc aaactcgtca
     4801 ctgcatgcaa acaataaagt cgcatgccct tttggcaata caagccttat gccatccttg
     4861 tcaacctgac atttatatac cttgttgttc caaatatcgt attcatagaa gttctcaaac
     4921 ctgttattct taaagaatac ctttgtatcc acatcttttt ccatggacaa gttgaaaaag
     4981 aagtaggcat caccatcatc ttcagacaag tgtaataccc ttgtaaatgg attcgtatcc
     5041 tttagttcca catcgacaaa tcctttttcc ctaaagaaag cagcaacctt gtctgtagga
     5101 acaactatgc aattttcaac aagttttctg gtgtgttcac gaattgcatt taactcttcc
     5161 tcactttcaa agttcttttc cgtcctgtct ggatagtctt ccgtgaatac tacgctaagg
     5221 ccttcactgg aaaaagaagc gcaaatatca aggagtttct tagggagcac ttcactgtag
     5281 gaaatgacaa gtgcatcaaa cttctcttca tttacaatca attttttatc ctttacagta
     5341 gcattcacca aagcatcaat agacactata tcaaaatcaa tctgctcttt tgcaagattc
     5401 gttgccacgt cctggaacag tctgtacttt ccgccacacc attctccttc agcattatag
     5461 aatactgcag cgtttgcaac atgcacacct ccgtaaagaa catgacttgc cctttgcaaa
     5521 tactccataa gcagtctgaa caatggaaac tgaggatttc ttccattggc gtaaaaatgc
     5581 ggagggcagt cagggtcatt gtcctttgcg gtaaaagcat ggggtacaaa atgattgatg
     5641 ccacctgcaa gcatcaaatc agtaagatac ttcatcaccg gcagcccttc agcaaacccg
     5701 tatgcgccgt aaagctccac catggctctg ttctttttcc ttggatccag atgcgcacat
     5761 gaagctccca gctttggcaa agtgttgata aagaattcag ggtcggcgac acctcttgaa
     5821 gagctgcttt tattaagcat gtcagttatt ccaggcaata tctgatgaag aaccacatcg
     5881 atgccactca tgtcctgtcc atcctgggct ctgaaaaaat gtcctgcacc gtatccaagt
     5941 ctcatgtggg tgttcatgtc ttctatgaca tgacctatgt acaaaacgcc tctttccctg
     6001 caccagttac caaccattct actgaagtta tccctgtaaa gatgggaaac tgtctccatg
     6061 tatgcatatc ttaattgtga agtctttcca gcaaccgtat accacaagca aggcaacaga
     6121 agctctgctt cttctgcaga gaggtttgcc ctctccgcca gcatttcgat catgtcattt
     6181 cgccagggaa gtatcagact tggtattcca agggtatcgt aatatgaacc tatattattt
     6241 gcaaaacatg gttcatcaga aaagaagccc ctgaaggtat tcccaaaata tttgccaaaa
     6301 cgctcataat gaggctcata aatctccttt atcataacct tgcaggactc ttcatcgcac
     6361 atgtctatgt aatttttgaa atgcgcaggc cctctctggg ttttaattac aaagaaaacc
     6421 ctccagacac cttccgggac atcccagtag acaagcccgt tttgcacatt gttagtaagc
     6481 agaactccgg tacttccgtc cacttcctca tctttgcctg ttctcctata agcagatact
     6541 gatattagtt cctctgtatt cttatcatat ctgtcagcaa taaatgcaga accgattaca
     6601 ggacccgcca catccaaaca ctctatgcgg acgcacttcc ttctcaaatg aggatatttc
     6661 ttttcaattg cgccatttgc ataacctgag ggaaagtgtt tgtcatcaag gagccaaacc
     6721 ctcatgtccc tatgcctggc ttgctcaagc ataaaagcaa aatcctccca ccatttgtct
     6781 ccgcaaaaat ctgcataggg gcggctttca aaacatattt cccttattcc gcttttgtaa
     6841 acagcatcaa tttcctcaac caatctgtcg tgttcttcac catgcagcca caaaaaagga
     6901 aagatgtagc ttctatcctc ttttgcattc aaacaatcat gaattctcct taccatcgtc
     6961 tcctcctaca ccctttattt agttaaacct gactcttttt ttatctcctt cattttcttc
     7021 agatttttct cacttatggt tccaactact tcgaactcat cctttatact ggttttaccc
     7081 tttaccaaaa accttgcaat ccttgcaacc caaaac
//
LOCUS       DAONVY010000044         7056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8061_length_7056_cov_3.289959, whole genome shotgun sequence.
ACCESSION   DAONVY010000044 DAONVY010000000
VERSION     DAONVY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8061_length_7056_cov_3.289959"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1273
                     /locus_tag="PLI11_02070"
     CDS             155..1273
                     /locus_tag="PLI11_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPZ51681.1"
                     /translation="MIYFDNAATSWPKPPEVSKSVLEAIEKAGNPGRGAHESALWSSY
                     KLYETREKIASLFCIREPLRVAFTLNATHALNAAISLCMGEIITTSMEHNSVLRPLAK
                     RGFYNIIQADQKGRLDPERLIDAMSGITGAVVMTHASNVTGIVNDIKVVGEACRKKGI
                     LFIVDAAQTAGVIDINVNEMNIDILCFTGHKGLFGLQGTGGIYVAPHVRLKPFMHGGS
                     GSRSFDINHPVDMPECFEAGTMNTHGIAALNAGVEFVQKVGVRNIERHEARLREYFVS
                     KVSDIPGVRIYDDSRCRHVGVVSITIPGADCSSISNYLSKRGIATRPGFHCAPLAHKT
                     IGTEETGTVRFSFGYFNKFEEIDYACNVLEEYIKEHVG"
     gene            1386..>3067
                     /locus_tag="PLI11_02075"
     CDS             1386..>3067
                     /locus_tag="PLI11_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51682.1"
                     /translation="MKGLKIAFYAALMILVVFTAGCSINTDINDVEPTNMPVPTNRPR
                     HDAPYYRNLVEGQAVEVPMYPEEHGEMTPFGLNVSDTVCIQFYAVTDFNKISLVCPSY
                     NNSIGTLKMSLYKWNINVDISLSGEVIHEQLFVNYKDNETLHFEFEPQEEGEYMLEIS
                     GIEELTGVWGTTAVRPNIRVYYNGQQQDASPFGSIGYLNTPDVHFGPLSQNIPYYFDY
                     VKAPAEIQFGDNHPVKMLDVRPDSFYAIDRLNRKLPSYKDYPEKHDKTRNVGILYYLK
                     PEKVGSVLTVKRILERFPDAISSSHGIWLPVRNLYWETPLYGNYNGINTNVYRKHAEL
                     LANAGVDFVVVNLADGLDMKILHIFMQSWEKAISEGVNAPKVVFATDYNDPGRGVIQL
                     KQLFKEIYKAEKYRELWFYYEGKPLILSNYDVLNTEFRNEQEIKQYFTMRRISTSYFD
                     DRTEEGVWGIQSVYPQTRHSMSRMGDVEQMVVSPAQNANYLGLTAMNGKEVFGRGYAK
                     GEYSYTYTYMGHEMVVDKNRQDAYLYGLNFQQQWDYALYVDPDFVLVNGWNE"
     assembly_gap    3068..3077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3078..3781
                     /locus_tag="PLI11_02080"
     CDS             <3078..3781
                     /locus_tag="PLI11_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51683.1"
                     /translation="NNWMGIDNAFVNQFNDEFSTDIEPVNGDLMDNYYYQLVANIRRY
                     KGVGSIDYEAARKSINIFDDISQWEDVPTYNHYINTASGVRNNILKTKVAYDRNTIYF
                     YVETRENLSPHTDQSWMRLYIDTGETNKHWEGFEFVVNRLSPNEDVCYLEVSAGGFNW
                     EIVGEVKYRVEGKVLQIEIPRHMLGMKSGWFSGMPSFNFKWADNVENDGDIFEFYKTG
                     DVAPIGRFTFRFNTD"
     gene            complement(3782..4882)
                     /locus_tag="PLI11_02085"
     CDS             complement(3782..4882)
                     /locus_tag="PLI11_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HPZ51684.1"
                     /translation="MNDRTKNVINIFLFTALLIGTLIANIIKKPDEVSVSERRKLARF
                     PKASLASILNGDFANGFSKYVRDQFVLRDEFRSVKAFVHFNIYRQKDNNNIYIADGSV
                     IKIDYPLNEKSVQNAAKKFNTLYEKYFEGMNVWYSIIPDKNYYTAEKYGYPHIDYNKM
                     IQILTSSVNNMIYIDITNALSIEDYYKTDLHWKQENLGNVIDVLSKEMNFQTKINLDN
                     YTKNVKEPFYGVYYGQSALNIKPDRMIYLTNEAMKDITVFYFDTNEFGEIYQKEKFSG
                     IDPYNLFLSGPAAVIVMENKNAKNDKELVLFRDSFGSSLAPLLTEEYAKITLVDLRYI
                     DSNYLGKVVEFTNQDILIMLNTQTINNSYMLR"
     gene            complement(4893..6284)
                     /locus_tag="PLI11_02090"
     CDS             complement(4893..6284)
                     /locus_tag="PLI11_02090"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPZ51685.1"
                     /translation="MLFSSIDFIYYFLAILIVVYFVTPNKFRNLLLLLASLIFYWYGE
                     PFYTLIMLFSALSAYIHGLLIHKYNKKVFLFTGVTINFSLLLFFKYTDFFIQNINNFL
                     KADIKLLKLALPLGISFYTFQTVSYMIDVYRKDTIAQKNPIKLFLYVTFFPQLIAGPI
                     VRYTDIEKELSYRTHSLSMISKGITRFVIGLGKKILIANVLGELCGIFRKSDEKSILF
                     YWIFAFSYMLQIYFDFSGYSDMAIGLGHMFGFNFPENFNYPFMSKTITEFWRRWHMTL
                     GTWFRDYVYIPLGGNRVSKAKWIRNIAIVWFLTGFWHGAGWNFILWGVFFAVLLVLEK
                     LFILRLLEKLPGIVSRIYVIILLATSFVIFNGDTKDLLTGDLKGMFGISQIPLYNTTT
                     LYYLRSYLVLLIIAAISSTHLMKTLVSRLKDRHNARLVLNIIEPVFVLVLMIAITAYL
                     VDGSFNPFLYFRF"
     gene            complement(6306..6878)
                     /locus_tag="PLI11_02095"
     CDS             complement(6306..6878)
                     /locus_tag="PLI11_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51686.1"
                     /translation="MKKLMLILLSVILVVSIAACARDNNNGDDDPKATEEPKGTEKPK
                     TEGNLEGSLEELIEKIYENADVDYSNIELVNTEVTEETCEYFTGVADLEYEEALASEP
                     AINPGAHSLVLIIMPEGADIDKARQEILEGADPFKWVCVGVEKVIVNNAGNLIILIMS
                     NDADALHDSFLELAGDLAGEPISKEGKAQE"
BASE COUNT         2133 a         1243 c         1557 g         2113 t
ORIGIN      
        1 aattgaatat gaaggaaccg aagacgttct tgcatgcata agtgcaggca aaaacaagct
       61 tgaagatatt attaaggaaa tctaagacct taaccttttg tttgctcgta tcatattata
      121 ttgtatgaat aaggagcaaa taagaggtgg tagattgata tattttgaca atgcggctac
      181 atcttggcca aaaccacctg aagtctcaaa gagtgttttg gaagctattg agaaggcagg
      241 gaacccaggc aggggcgccc atgaaagtgc gttatggtca tcctataagc tgtatgagac
      301 aagagaaaaa atagcgagct tgttttgcat aagagagcct ctaagagtcg cattcacctt
      361 gaatgcgacg catgcattaa atgctgcaat tagtttgtgc atgggtgaga ttataactac
      421 atccatggag cataattccg tacttagacc tcttgcaaaa agaggttttt ataatataat
      481 tcaggcggat caaaagggaa ggctggatcc ggaaaggttg attgacgcaa tgtcgggaat
      541 caccggtgcg gttgttatga ctcatgcatc caatgtcaca ggaatagtca atgacattaa
      601 agttgttgga gaggcatgca gaaaaaaagg aatcctgttt attgttgatg cggcacaaac
      661 agcaggagtg attgatatca atgtcaatga gatgaatatt gacatattgt gttttacagg
      721 gcacaagggt ttgtttggcc tgcagggaac aggtggcata tatgtggcgc cacatgtaag
      781 gctgaagcct ttcatgcatg ggggatccgg aagcaggagt tttgatatca atcatccggt
      841 ggatatgcct gaatgttttg aggcaggaac aatgaacacc catggaatag ctgcattgaa
      901 tgcaggagtg gaatttgttc aaaaggttgg agtaaggaat attgaaagac atgaagcaag
      961 gcttagggaa tactttgtta gcaaggtgtc cgacatacct ggagtaagga tatacgatga
     1021 ttcaaggtgc aggcatgtgg gcgtagtttc aataaccata ccaggagctg actgcagttc
     1081 catatccaac tacctttcca aaagaggaat agcaacccgt ccaggttttc attgtgctcc
     1141 tcttgcgcat aaaacaatag gaactgaaga gaccgggacg gtgagattca gctttggata
     1201 ttttaataaa tttgaggaaa ttgattatgc atgcaacgtg cttgaggaat acataaagga
     1261 acatgttgga tgataaaacg gtgttaattc accgtttttt ataactttct ttgcaatttt
     1321 gtagtaatta aagcaattct catgttataa tggtcatgaa attgccaaga ggaggataac
     1381 tctagatgaa aggcttaaag attgcatttt atgcagcttt aatgatactt gttgttttta
     1441 cggctggttg cagcatcaat acggatatta acgatgttga acctacaaac atgcctgttc
     1501 caacaaacag gcctagacat gatgcaccat attatagaaa tcttgtagaa ggacaggcag
     1561 tggaagttcc catgtatcca gaagagcatg gtgagatgac gccctttggg ctgaatgtca
     1621 gtgacactgt ttgcattcaa ttctacgcgg ttactgattt taacaaaata agccttgtat
     1681 gtccgagcta taacaacagt atcggaactt tgaaaatgag tctgtacaaa tggaatatta
     1741 atgtagacat cagcttgagc ggagaagtca tacatgaaca gctgtttgta aactataaag
     1801 acaatgaaac actgcatttt gaattcgagc ctcaagaaga gggcgagtat atgcttgaga
     1861 taagtgggat agaggagctt actggagtat gggggactac agctgtaagg ccgaacataa
     1921 gggtgtacta taacggacaa cagcaggatg catctccttt tggaagcatt ggttatctga
     1981 atactccaga tgtacacttt ggacccttgt cccaaaacat tccctactat tttgattatg
     2041 taaaagcgcc tgctgaaatt caatttggcg acaaccatcc agtaaaaatg ttggatgtaa
     2101 ggccggattc tttttatgct attgacagac ttaacagaaa actgccttct tacaaggatt
     2161 atcctgaaaa acacgacaag acgagaaatg taggcatatt atactatcta aagccggaga
     2221 aggttggctc tgtccttact gtcaagagaa tacttgaaag atttcctgat gccatatcct
     2281 catcccatgg aatctggctg ccggttcgaa acttgtattg ggagaccccg ttatatggaa
     2341 attacaatgg tattaacacg aacgtataca gaaagcatgc tgaacttctt gcaaatgcgg
     2401 gtgttgactt tgtggtcgta aatcttgcag acggccttga tatgaagata ctgcatatct
     2461 tcatgcaaag ctgggaaaag gcaatatcgg agggagtaaa tgctcccaag gtagtgtttg
     2521 caacagacta caatgatcca ggacgaggcg ttatacagct caaacagttg ttcaaggaga
     2581 tatataaagc ggaaaaatac cgtgagctat ggttctatta cgaaggaaaa ccattgattt
     2641 tgagcaatta tgacgtattg aacacagagt tccgtaatga gcaggaaata aagcagtatt
     2701 ttacaatgcg caggataagt acatcttatt tcgatgatag gacggaagaa ggtgtttggg
     2761 gtatccaatc agtatatccg caaaccagac acagcatgtc caggatgggg gatgtggagc
     2821 aaatggtggt aagtcctgct caaaatgcca actaccttgg acttaccgca atgaacggaa
     2881 aagaagtgtt tggaagggga tatgctaaag gagagtattc ctatacatac acgtacatgg
     2941 gtcatgaaat ggttgtagac aaaaacaggc aggatgcata tctttatggt cttaacttcc
     3001 agcagcagtg ggattatgct ctctatgtcg acccggactt tgtgctggta aatggttgga
     3061 acgagttnnn nnnnnnnata acaactggat gggaattgac aatgcgtttg taaaccagtt
     3121 caacgatgag ttcagtactg acatagagcc tgtaaatggt gaccttatgg ataattacta
     3181 ctatcagctg gtggcaaaca taaggagata caaaggtgtt ggaagtattg attatgaggc
     3241 tgcgagaaag agtataaaca tatttgatga tatttcccag tgggaggatg ttccgactta
     3301 caatcattac atcaataccg caagtggtgt aagaaacaat atacttaaaa caaaagtagc
     3361 atatgacaga aacaccatat acttctatgt ggagaccagg gagaacttga gtccgcatac
     3421 tgatcagtcc tggatgaggc tgtatattga cacgggagaa accaacaagc attgggaagg
     3481 tttcgagttt gtggttaaca gactgagtcc taatgaggat gtttgctatc tcgaggtgtc
     3541 tgccggaggc ttcaattggg aaattgttgg agaggtaaaa tacagggttg aaggcaaggt
     3601 tctgcaaata gaaataccca gacacatgct gggtatgaaa tccggatggt tttcaggaat
     3661 gccgtcattc aacttcaagt gggcggacaa tgttgaaaac gatggtgaca tctttgagtt
     3721 ctacaaaaca ggagatgtgg cacctatagg aaggtttact ttcagattca atacagactg
     3781 attagcgaag catgtagctg ttgttgatgg tttgagtgtt cagcataatc agtatgtcct
     3841 gattggtaaa ttccacgacc ttgccgagat agtttgagtc gatgtatctc aagtcgacca
     3901 gcgttatttt ggcatattcc tcggtaagga gcggagcaag actgcttccg aaagaatctc
     3961 tgaacagaac aagttccttg tcattttttg catttttgtt ttccatgact attacagcgg
     4021 cagggcctga gagaaaaaga ttatacgggt cgatgccgct gaatttttct ttctgatata
     4081 tttcaccaaa ctcgtttgta tcaaagtaga atacagttat gtctttcatg gcttcgttgg
     4141 taaggtaaat catcctgtcc ggctttatat tcaaggcaga ttgcccgtaa tatacgccat
     4201 agaatggttc ctttacattt ttcgtataat tatcaaggtt aatctttgtt tgaaaattca
     4261 tttcttttga taaaacatca ataacattgc ccaggttttc ctgtttccaa tgcaggtcgg
     4321 ttttgtaata gtcttctatg gatagggcgt ttgttatgtc tatatatatc atgttgttta
     4381 cactgctggt caagatctga atcattttgt tgtagtctat atggggatat ccgtattttt
     4441 ctgcggtata gtagtttttg tcgggtatta tggaatacca gacattcatg ccttcaaagt
     4501 atttttcata tagtgtgtta aatttcttcg ctgcgttttg gactgatttt tcattcagtg
     4561 gataatctat ttttattaca ctgccatcag caatatagat attgttgttg tccttttgcc
     4621 tgtatatatt gaaatggaca aaggctttaa cacttctgaa ttcatctctg aggacaaact
     4681 ggtcccttac atatttggaa aacccgtttg caaaatcacc attaagaata cttgccaaac
     4741 ttgcttttgg aaaccgggca agttttcttc tttcacttac tgaaacctca tccggttttt
     4801 ttataatatt tgcaatcaaa gttccaatca gcaatgcagt aaaaagaaat atgttgatta
     4861 catttttcgt tctgtcgttc atagtgtcct ccttagaatc taaagtacaa gaacggattg
     4921 aaggaaccat ccaccagata tgctgttatt gcaatcatta acaccaggac aaatacaggt
     4981 tcaataatat ttaatacaag ccttgcattg tgtctgtctt tcaaccttga aacaagtgtt
     5041 ttcatcaagt gtgtagatga tatagcagca attatcaata gaaccaaata gcttcttaga
     5101 tagtacagtg ttgtggtatt gtacagggga atttgggaga tgccaaacat cccttttaaa
     5161 tctcctgtca aaaggtcttt tgtatcgccg ttgaaaatga caaagcttgt tgcgagcagg
     5221 atgattacgt atattcttga aacaatacca ggcagctttt caagaagcct taagataaaa
     5281 agcttttcca gaacaagcag cactgcaaag aatacacccc aaagaatgaa gttccagcca
     5341 gctccatgcc agaatcctgt caagaaccat acaattgcta tgtttcttat ccattttgcc
     5401 ttgcttaccc tgtttcctcc cagtggaatg taaacatagt ctctgaacca ggtgcccagg
     5461 gtcatgtgcc atctgcgcca gaactcggtt atggtttttg acatgaaagg atagttgaag
     5521 ttttccggga aattaaaacc aaacatgtgc ccaagtccga ttgccatgtc gctgtaacct
     5581 gaaaagtcga agtatatctg cagcatgtag ctgaaggcaa atatccagta aaagagtatg
     5641 gatttttcat ctgactttct aaaaatccca caaagttctc ccaacacatt ggcgataagt
     5701 attttctttc ccagtcctat tacaaacctt gtgatacctt ttgatatcat cgacagggaa
     5761 tgcgttctgt atgacagctc cttttctatg tcggtatagc ggactatggg gcctgctata
     5821 agttggggga agaaggtcac ataaaggaaa agctttatgg ggtttttctg tgcgattgta
     5881 tcttttctgt atacgtcaat catgtagctt acagtttgga aggtgtaaaa gcttattccc
     5941 aaaggcagtg caagctttaa taactttata tccgctttta gaaagttgtt aatattttgt
     6001 atgaaaaagt cggtgtattt aaagaatagc aaaagggaga aatttatagt tacccctgta
     6061 aacaggaata cttttttatt gtatttatgt ataaggagac cgtgtatata ggcagacaag
     6121 gcagaaaaga gcatgattag ggtgtaaaat ggctctccat accagtagaa tatcagactt
     6181 gccaaaagca gtaacaggtt tctgaattta ttcggagtga caaagtatac tacaatgagt
     6241 atagctagga aataatatat aaagtctata ctggaaaaaa gcattttaca ccctcttttc
     6301 tgcaattatt cctgagcttt gccttccttg ctgattggct cgcctgcaag atctcctgca
     6361 agctccagga agctgtcatg gagtgcgtcc gcatcgttgg acatgatgag gattataagg
     6421 ttgcctgcgt tgttgactat gaccttttct actcctacgc atacccattt gaatggatcc
     6481 gcaccttcaa gtatttcctg tcttgcctta tctatgtcag cgccttcagg cattatgata
     6541 agtaccagtg agtgtgctcc aggatttatt gcaggctctg aagccaatgc ttcttcatat
     6601 tcaaggtctg caacaccggt aaagtattca caggtttctt ctgttacttc tgtgtttacc
     6661 agttcgatgt tggagtaatc cacatctgcg ttctcataga tcttttcaat gagttcttcc
     6721 aatgaacctt cgagatttcc ttctgtcttt ggtttttcag ttccttttgg ttcttcggtg
     6781 gcctttggat catcatcacc gttattattg tctcttgcgc atgctgcaat agagacaaca
     6841 agaattacac ttaatagaat cagcataagt tttttcatac tatcaccttt ctttggttaa
     6901 atgttgtaca tttataaaac tgttgaggag gggttgaatg ttgcgtttat gaaaagttta
     6961 tttgtataaa ttttattaaa actgttaatt aataaatggg tgtgtcaacc gtatttgaaa
     7021 atgtccccaa aaaaataaga aaaaacagca taagaa
//
LOCUS       DAONVY010000045         7053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8072_length_7053_cov_3.354101, whole genome shotgun sequence.
ACCESSION   DAONVY010000045 DAONVY010000000
VERSION     DAONVY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8072_length_7053_cov_3.354101"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLI11_02100"
     CDS             <1..334
                     /locus_tag="PLI11_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ51687.1"
                     /translation="PDGDVRTVLRFHPYLAPVKIAVLPLSKKLSDEAYQVYEMLIRKY
                     YCEFDETGSIGKRYRRQDEIGTPYCVTFDFDSREDNKVTIRDRDTMEQVRVAMDELIP
                     WFEDKFDF"
     gene            402..3023
                     /gene="ppdK"
                     /locus_tag="PLI11_02105"
     CDS             402..3023
                     /gene="ppdK"
                     /locus_tag="PLI11_02105"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPZ51688.1"
                     /translation="MKYVYLFTEGNAGMRNLLGGKGANLAEMISLGLPVPNGFTITTE
                     ACTRYYADDETIAPEIIDEIYASLAKTEEMVGKKFGDPNNPFLVSVRSGARASMPGMM
                     DTILNLGLNDEVVEGLAKLTGNERFAYDSYRRFIQMFSDVVMGIEKPKFDKILDGTKE
                     AKGVVLDTELDTEDLKGVVRKYKELYKEEMGEDFPQEPKTQLINAVEAVFKSWNNERA
                     IYYRRMHDIPSEWGTAVNVQEMVYGNMGEDSGTGVAFTRNPSTGDNELYGEFLMNAQG
                     EDVVAGIRTPEPISHLKEVNPDVYKQFMDIAEKLENHYKDMQDLEFTIERGKLFILQT
                     RNGKRTAQAALKIAVDLVNEGLITKEEAVMQVDPKQLDALLHPTFDPDALKKASPIAK
                     GLPASPGAATGRIYFTAEDAKNAFDSGVEQVILVRLETSPEDIEGMNISQGILTGRGG
                     MTSHAAVVARGMGRCCVAGCSEITINEEGKTFTDKSGNVYKEGDWISLDGSTGNVYAG
                     QIATKNPELTGDFATLMAWADEFRTLGIRTNADSPKDAEVARSFGAEGIGLCRTEHMF
                     FEEGRIFAFRRMIVANNEEQRKDALKEILPMQRQDFEGIFKAMNGLPVTIRLLDPPLH
                     EFVPQDEGEIKELAEAVGISVSELKARIDNLHELNPMLGHRGCRLAVSYPEIAIMQTT
                     AIIEAAINVTKEGLNVVPEIMIPLVSESKELKFVKNVIKTTADKIIAESGVNVEYKIG
                     TMIEIPRACLTADEIAKDADFFSFGTNDLTQMTYGLSRDDAGRILEDYYNAKIFESDP
                     TARLDVTGVGKLVEMAVGLGKQTNPNIKLGICGEHGGDPSSIEFCHKVGLTYVSCSPF
                     RVPIARLAAARSAIANK"
     misc_binding    3119..3345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3395..5107
                     /locus_tag="PLI11_02110"
     CDS             3395..5107
                     /locus_tag="PLI11_02110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPZ51689.1"
                     /translation="MKLDRLVGERFKERPSDCVIDSHALMIRGGYMKQVSNGIYSQFM
                     PLKRIAHKVERIIREEMDAIDGQEVLFPVVMPASLWEESGRYESVGKELARFVDRNEK
                     KLILGMTHEEACVHLVKEYGNSYTKYPFMVYQIQTKFRDEARPRAGLIRVREFTMKDA
                     YSFHTSQEDLEEYYERCYEAYLRIFARAGIPDVVAVSSDPGMMGGNISHEFMLLTPVG
                     EDTVVLCTECDYKANMEAAESIVENTRDEVSKELELVSTPATKTIEALCDFLGITKEK
                     TCKAVMYRRNIDDKIIVVFIRGDLDVNETKVRNYLGFEIHPADIEEGEGIVPGFTGPV
                     NLSGEIIVLFDKSLENTNNMVAGGNVEDYHYVGLDFARDLKDVEFHDLAKAVEGGICP
                     QCGNKSLTVKRGIEVGNIFQLGTKYTESMGMEYIDSDGKPRNPIMGCYGIGVGRLIAS
                     VAEVHHDENGPIWPMSISPWQVHICALRSDEPNVKEFADKLYENLMKKGIEVIYDDRN
                     VSAGVMFSDADLLGVPVRVIVSPRNLKNNVCEITARDKSFTIQEPIESVEEKLVEIVK
                     SNLC"
     gene            5293..>7053
                     /locus_tag="PLI11_02115"
     CDS             5293..>7053
                     /locus_tag="PLI11_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ51690.1"
                     /translation="MQKRIMLLVVVVFLCLGLFSCGKKEPTDGDQEEEVKGYGAGEIV
                     ISVFWPPMKGFTTEEQFDYLVEAGIDLLEWGTDPIFTDEDTIRETLRLCNEKGLKITI
                     ADKDWIGIEKKSDKEIRELVRRYKDNECVVGYLLLDEPHNANPFGRVAKIMAEEHPGC
                     ISQLNMLPMGALPDPRGHAEDWISSAGRENVRYLSYDQYPFALQPGSIPQMFGNMNFV
                     REVGLKYNVDTALYIQSVGVLDKFRRTNANEIRYHTSAALAYGYKNLKYFTWITPVER
                     DEDFTLAIISPEGEKTDLFDGVAQINRDIKKVSSILGKLDAVEIYHNGRQDASTKMLE
                     PGWHVEATDKKDFLVSLMVDRKTKRNYLMIVNKNFNKDATLALKLNGVESLMDVTSGE
                     EEEVTITDGIFQCELLAGGFRLYRLSEGVTLHKEYQDADVNLALDKPIYSNFSRGYDG
                     YFNYKAVDGNRVSTDRSRGWRYEGAGDEEIYIMVDLKRAVDINRVDLYPVTSGDEERN
                     GQYFPRKFTILYSTNGKDYKEILSDTWEKRKELSYTFDTVKARYVKIRIDEAVKVSDI
                     YIAEICEIEIYNDDGTLPKYQ"
BASE COUNT         2165 a         1233 c         1798 g         1857 t
ORIGIN      
        1 gcctgatggc gatgtaagga ctgtgcttag attccatcca taccttgcgc ctgtcaagat
       61 tgcagtactg cctctttcaa agaagctttc agatgaagct taccaggttt atgaaatgct
      121 tatcaggaag tactactgtg aatttgatga aacaggcagc ataggcaaga ggtacagaag
      181 gcaggatgaa attggtacgc cttactgtgt cacctttgac tttgattccc gtgaagacaa
      241 caaggtgaca atcagggaca gagacacaat ggagcaggta agagttgcaa tggatgaact
      301 tattccatgg tttgaagaca agtttgattt ttgacaagcc agtatcaatt attcattgat
      361 tatatagatg ctaaaactaa aatattatat ggaggtaatg tatgaaatac gtatatttgt
      421 ttactgaagg caatgctggt atgagaaatt tgcttggcgg caaaggcgca aaccttgctg
      481 aaatgatatc tctaggattg ccggttccaa atggttttac aattaccact gaagcatgta
      541 caaggtacta tgcagacgat gaaaccatag ctccggagat tattgatgag atatatgctt
      601 ctttggccaa gactgaagaa atggttggca aaaagtttgg cgatcccaac aatcctttcc
      661 ttgtttcagt tcgttccggt gcaagagcat caatgccagg catgatggat acaattttga
      721 atctgggact aaacgacgaa gttgttgaag gacttgcaaa gctgacaggc aatgaaagat
      781 ttgcatatga cagctataga agattcatcc agatgttttc agacgttgtt atgggaatag
      841 agaaacccaa gtttgacaaa attcttgatg gtaccaagga agcaaaaggt gttgttcttg
      901 acactgagct tgacacagaa gatctcaagg gagttgttag gaagtacaag gaactctaca
      961 aagaagaaat gggcgaagat ttcccacagg agccaaagac ccagcttatc aatgcggttg
     1021 aagctgtttt caaatcctgg aataacgaaa gagccatcta ctacagaaga atgcatgaca
     1081 ttccaagcga atggggtact gcagtcaacg ttcaggaaat ggtttacggc aacatgggag
     1141 aagactctgg tactggtgtt gcatttacaa ggaatccatc cactggtgat aacgagcttt
     1201 acggcgagtt cctaatgaat gctcagggtg aggacgttgt tgcaggtatc agaactcctg
     1261 agccaatatc tcatttgaag gaagttaatc cggatgtgta caagcagttt atggatatag
     1321 ctgagaagct tgaaaaccat tataaggaca tgcaggacct tgagtttact atagagagag
     1381 gaaaactgtt tattctgcag acaagaaacg gtaagagaac cgctcaggct gctttaaaga
     1441 ttgctgttga cctggttaat gaaggtctca tcacaaagga agaggctgta atgcaggttg
     1501 atcctaaaca gcttgacgca cttctgcacc caacctttga tcctgatgca ttgaagaagg
     1561 catctccaat cgcaaagggt ttgccggctt caccaggtgc tgcaacaggt agaatctact
     1621 tcacagctga agatgcaaag aatgcatttg acagtggtgt tgagcaggtt atacttgtaa
     1681 ggcttgagac atcacctgag gatattgaag gaatgaatat ttcacagggt atcctcactg
     1741 gaagaggcgg aatgaccagc cacgctgcgg ttgttgcaag aggcatggga agatgctgcg
     1801 ttgcaggatg ctcagaaatc actataaatg aagaaggaaa gaccttcaca gacaagagtg
     1861 gcaatgtata caaggaaggc gactggatct cccttgacgg ttccacaggt aatgtatatg
     1921 ctggacagat tgctaccaag aatccagagc ttactggaga ctttgcaacc cttatggctt
     1981 gggctgacga gttcagaacc cttggaatac gtaccaatgc ggattcacca aaggatgctg
     2041 aagttgcaag aagctttgga gcagagggta tcggactttg ccgtactgag cacatgttct
     2101 ttgaggaagg cagaatcttt gcattcagaa gaatgattgt tgcaaacaac gaagagcaga
     2161 gaaaggatgc tttgaaggaa atcctgccaa tgcagagaca ggacttcgaa ggcatattca
     2221 aggctatgaa tggtctgcct gtaaccatca gactgcttga tccaccacta catgagttcg
     2281 tacctcagga tgaaggagaa atcaaggagc tggcagaggc tgtaggcata tctgtaagcg
     2341 agcttaaggc tagaatagac aacctgcatg agctcaaccc aatgcttggc cacagaggat
     2401 gccgtcttgc agtttcatat cctgagattg caatcatgca gacaactgct atcatcgagg
     2461 cagctatcaa tgttaccaag gaaggcttga atgtggttcc tgagatcatg attcctcttg
     2521 taagtgaatc aaaagagctt aaattcgtga agaatgtaat taagacaact gctgacaaga
     2581 ttatagctga aagcggagtc aatgttgagt acaagatagg taccatgatt gagattccaa
     2641 gagcatgtct gactgctgac gagattgcaa aagatgcaga cttcttctcc ttcggaacca
     2701 acgacctgac ccagatgact tacggtttga gccgtgacga cgctggtaga atactcgagg
     2761 actactacaa tgccaagata ttcgagtcag atccaacggc tagactggat gtaacaggtg
     2821 tgggcaaact ggttgagatg gctgtaggtc ttggcaagca gacaaatccc aatatcaagc
     2881 ttggtatttg cggtgagcac ggcggagatc catcctccat cgagttctgc cacaaggttg
     2941 gactgactta cgtttcatgt tcaccattcc gtgttccgat tgctaggctt gcagctgcaa
     3001 ggtccgcaat tgctaacaag taatacaaag ataattcagg gcgtgtctat tatggacatg
     3061 cccttaattt attgactaaa ctattttttt gtggtatata ttattggtaa ttgaataatc
     3121 aatggctttg atgagaagca gtagatattt cttgtgattg tagagagaag gcggttggtg
     3181 caagcctgaa tcagggagat atcgaacccg tctttgagct gtgatttgga acgaataccc
     3241 ttggtattcc agtaaatatc accgggaact cccgttacag ggtacaatga gtgcggaaat
     3301 accgaagtta ggtggtacca cggactatga atgttcgccc taagctgatt atcagattgg
     3361 ggttttttta atatttattt ttggaggtat atgtatgaag ttggacagac ttgtagggga
     3421 aaggtttaag gaaaggcctt ccgattgcgt catcgattct catgctttga tgatacgcgg
     3481 aggatatatg aagcaggttt caaacggaat atattcgcaa ttcatgcctc ttaagagaat
     3541 tgcacacaag gttgagagaa taattcgcga agagatggat gcaatagatg gacaggaagt
     3601 attgtttcct gtggttatgc ctgcatccct ttgggaagag tcgggcagat acgaatctgt
     3661 gggcaaggaa cttgccaggt tcgttgacag gaacgaaaag aagctcattc tcggcatgac
     3721 tcatgaggaa gcatgcgtgc atcttgtaaa ggagtatggc aacagctata cgaagtatcc
     3781 attcatggtt taccagatac aaaccaagtt cagagacgaa gcccgtccaa gagcaggact
     3841 aattcgtgtc agggagttca ctatgaagga cgcatactcc ttccacacat cccaggagga
     3901 tttggaagag tattacgaaa gatgttatga ggcgtacttg aggatttttg cacgtgccgg
     3961 cattcctgat gtggttgcgg taagctccga tcctggtatg atgggtggaa acatatccca
     4021 tgagtttatg cttctcacac cagtcggaga agacacggtg gttctgtgca cagagtgtga
     4081 ctacaaggca aacatggagg ctgccgagtc cattgttgag aatacaaggg atgaagtatc
     4141 aaaagagctg gagctggtca gcactcctgc cacaaagaca atagaagcct tgtgtgattt
     4201 tctcggaata accaaggaga agacttgcaa ggcggttatg tacagaagaa atattgatga
     4261 caaaataata gttgtgttta tacgtggaga cttggatgtc aatgaaacaa aagtaaggaa
     4321 ttatcttgga tttgagatac atcctgcaga tattgaggaa ggcgaaggta tcgttcccgg
     4381 ctttacaggt ccggttaacc taagtggtga aataatagta ctgtttgaca agagtcttga
     4441 gaacaccaac aacatggtcg ctggcggcaa tgtggaagat taccattatg tcggtcttga
     4501 ctttgcaaga gatttgaagg atgttgaatt ccatgattta gcaaaggccg tagaaggggg
     4561 catttgtcct caatgcggta acaagtcttt gacagtaaaa agaggcatag aggtgggcaa
     4621 tatattccag cttggaacca agtacaccga gtccatgggc atggaataca ttgacagtga
     4681 cggcaagcca agaaatccaa tcatgggctg ctacggcata ggtgttggaa gactgattgc
     4741 ttccgttgca gaggttcatc atgatgaaaa cggtccaatc tggcccatgt caataagtcc
     4801 atggcaggta cacatttgtg ctctacgctc cgacgaaccc aatgttaagg agtttgcgga
     4861 caaattgtat gagaacctta tgaaaaaggg tatagaggtt atatatgacg acagaaatgt
     4921 tagtgccgga gtcatgttct ccgatgcaga ccttttgggt gtgcctgtaa gggtaattgt
     4981 gagtccgaga aatctcaaga ataatgtctg tgagataacg gcaagggaca agagcttcac
     5041 catccaggag ccgattgaaa gtgttgaaga aaaattggtt gaaatagtga aaagcaattt
     5101 atgctaggaa aaaacccaca gtttgtgggt ttttattttg atgaattgag ttaaaaaagc
     5161 attattgaga tattgaacag tactgaaatg ttttgattag ggaggtattc tcccctttat
     5221 attatttaca aaaagtaaaa taatattata attatcatat attaatttta aaattgttcg
     5281 gagtgtgtta ttatgcaaaa acggattatg cttttggtcg tggtggtttt tttgtgtttg
     5341 ggtctttttt catgcggtaa aaaggaaccc acggatggtg atcaggagga agaggttaaa
     5401 ggttatggcg caggagaaat agtcatttct gttttttggc ctccgatgaa aggttttacc
     5461 actgaagagc agttcgatta tttggttgag gctggtattg atttgctcga gtggggaacc
     5521 gatccgattt ttacagacga ggatacaatc agggaaactt taagattgtg taatgaaaag
     5581 ggtttgaaga taacaattgc agacaaagac tggattggca ttgaaaagaa atcggataaa
     5641 gaaattaggg agcttgtaag aaggtacaag gacaatgaat gcgtagtggg atatttgctt
     5701 cttgacgagc cacataatgc aaatccgttt ggaagagttg caaaaatcat ggcggaagag
     5761 cacccgggat gtatatcgca gttaaacatg cttcctatgg gagcgctgcc tgacccaaga
     5821 ggtcatgcgg aagactggat aagctctgcg ggtagggaaa atgtaaggta cctttcctac
     5881 gaccagtatc cctttgcctt gcagcctggc tccattcctc agatgtttgg aaatatgaac
     5941 tttgttaggg aagttggtct taagtacaat gtggataccg ccctttacat ccaatctgtt
     6001 ggtgtacttg acaagttcag aaggacgaat gcaaatgaga ttaggtacca tacatctgca
     6061 gcgcttgcat atggttacaa aaatctcaag tattttacct ggataacacc tgtggaaaga
     6121 gatgaggatt tcactcttgc cattatttca ccagaaggcg agaaaacgga tctctttgat
     6181 ggtgtggcgc agataaacag ggatataaag aaggtgagca gcattctagg aaagcttgat
     6241 gccgttgaga tatatcataa tggaagacag gatgcctcaa cgaaaatgct tgaacctggc
     6301 tggcatgtgg aagctactga caaaaaggat tttcttgttt ccttaatggt ggatagaaag
     6361 acaaagagaa actatcttat gattgttaac aagaatttta acaaggatgc aacccttgct
     6421 ctaaagctca acggtgttga aagtttgatg gatgtaacca gtggggagga agaggaggta
     6481 accataacag atggaatttt ccagtgtgag cttcttgcag gcggtttcag gctgtataga
     6541 ctttcagaag gtgtgacttt acataaagaa tatcaggatg cggatgtgaa tctggctttg
     6601 gataaaccaa tttactccaa cttctcccgc ggatacgatg gatactttaa ttacaaggcg
     6661 gtcgacggaa acagggtgtc caccgaccgt tccagaggat ggagatatga aggagcagga
     6721 gacgaagaga tttatataat ggtggatctt aagagggctg ttgatattaa cagggtggac
     6781 ctgtatcccg ttaccagcgg agacgaggaa agaaacggac agtactttcc aaggaagttt
     6841 acgatactgt actcaaccaa cggaaaagat tacaaagaaa tcttgtctga tacctgggaa
     6901 aagagaaaag agctgagtta tacatttgat actgtaaagg ccagatatgt gaaaatacgt
     6961 attgatgagg ctgtaaaggt ttcggatata tatattgccg agatatgtga aattgagatt
     7021 tacaatgatg atggtacact gccaaaatat cag
//
LOCUS       DAONVY010000046         6906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8271_length_6906_cov_3.711137, whole genome shotgun sequence.
ACCESSION   DAONVY010000046 DAONVY010000000
VERSION     DAONVY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8271_length_6906_cov_3.711137"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1856)
                     /locus_tag="PLI11_02120"
     CDS             complement(261..1856)
                     /locus_tag="PLI11_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51691.1"
                     /translation="MKKIILILSLLCVVIICGLIFIEEPGKVVAASNLQEMFDMEGGA
                     YQEKVSAEIHAVQVNALVPFTGGNAKFGTWGGEVSHAVELYKWDGDYATTIDGEVLAT
                     ANIPATPNDSFVNFTFLDDGGNVYTAESGEYLMVFSSTKGSTSYIYKITTPVKSYHKV
                     FLAGMEQDVTLSMKLNFTDVSDTYFGEVSESESGINYDFYLASNPKDVHKAYFVENNA
                     ASQFIATAPFNSIIINTPTWGGKVKSVRFALFKWDVDYKTTVSGEPVATYYNHFGGAS
                     DDPDTLFKFSKQEPGEYLLVVYNTEDNTDSERPYSIVWIINENFEGQRMYKDGKVDAG
                     KSLEFTIAYTRVPEKMYGAPLSSSVYIAEEIVFAGNQIRKSKLVPFKDGDNYNTSDLR
                     QGLRFCFDAPEVGSNITIGNTVYIVSEVGALVMLERNMAEGFEMLVENLASGAKMVKQ
                     SELVPWEDNGNIYNSAYVYNIPEAEKDTAICVRMYIKCEKEGKTVYAYSEVKKASVTD
                     VYQEIVNVGHEDMLDESVVMWWK"
     gene            complement(2026..2922)
                     /locus_tag="PLI11_02125"
     CDS             complement(2026..2922)
                     /locus_tag="PLI11_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPZ51692.1"
                     /translation="MLYTNADFENYAKDPAVVQFNGKYFLYHSIMIDGRLNIGISCSE
                     DGENFVRIANLPITQKCEKNGIGAPGCILVKGQIHLFYQTYGNNKLDAICHAVSSDGI
                     NFVKDESNPVFAPRMPWAKMPSWSCGRAIDADVCVFGDKMILYFATRDKDFEKQIVGA
                     AWAYLDSDFSAGDFYLHKEPVLKPELDWEMTCIEGPASVVIDGKVYMFYAGAYNCSPQ
                     QIGCAVSDDGFRFRRLSDQPFLSNGAPGDWNASESGHPYVYETDDGHLWLYYQGSPDM
                     GKSWYLSRRELLFDKGKFILKD"
     gene            complement(2955..3674)
                     /locus_tag="PLI11_02130"
     CDS             complement(2955..3674)
                     /locus_tag="PLI11_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51693.1"
                     /translation="MRKLFFSILALTLLVFVGCAKESEHQATPSPTPEATSNTTVEPT
                     DTPAPTEEATPAETATPLPTVEPGTNQYAGEIDETKAIKADFYEGGHVGQQAHKIFGS
                     FSVQFFATTTFNKVGISMPTWFQEEGHAATLSLYAWQGSYEATMMSEPVVTKEVADWK
                     DGVEVLIELDEELPDGEYLYEIYNEDTDNVCVWYKAGTVDYVRSYYNNEIWEAGTPRL
                     VVYYTKKPSNIHGPIQASGLE"
     gene            complement(3671..6121)
                     /locus_tag="PLI11_02135"
     CDS             complement(3671..6121)
                     /locus_tag="PLI11_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51694.1"
                     /translation="MSSRKFFLLCISLVLAVLLTVIAGKEGEEMHGGLIEGQRTSAVL
                     YNEGDKVVNLPMAANETLGIRFHATTEYDGVEFFVTNALIQRNSMQLFLYEWKGSYKK
                     TSASDAVVTQQISKFARNNWAKFEFDSTVGSGEYLAVLCNFTGNVSLAKIPSDKENVA
                     LYFNGSDMAGSLQARLNYIHTPKNNLAALSKTDETSLIGLARPGSYVATDGLDRTLPD
                     YNEVGPLRDGKYVGMFFWTWHEKYSASVPRNVTEILKKHPDILYDYSAEEWGGVHDSY
                     FWNEPIYGYYNGKDPWVFRKQAELLADAGVDVIIFDNSNRTATYIDGVKVLLETFAQA
                     RADGVKTPQISFLMNFYEGTWPNTVVQLRELYTEIYRPGKYKDIWFYWKGKPLMMAYP
                     DKLNPNDELEAEILEFFNFRPPQPSYVDGQTRPDQWGWCSIYPQQVYYNEDGTPEQIT
                     VSVAQNHNDKQGLVAMNGERVFGRHWMNAKKEYDSRENAILYGANFEEQFRYALEVDP
                     EFIFITGWNEWRAGRYEEWMGVPNAFPDQFNDAYSRDIEPSKGELKDHYYYQLVSFVR
                     RFKGVEKPEAASKGKTIDIYSEEDMWTDVKPYFASYGGNTLHRNSPGYLGYHYENTSG
                     RNDIVGAKVTHDNDFVYFMVETKENISSSTDPAWMRLFIDVEGQKGPNWETFEYIINR
                     VSPGEKAVLEKSNGGWNWEKVGDVEYSVKDNRLQIKVPKSMLGINGDKFVVNFKWSDN
                     MQNDGDVMDFYVNGDAAPGGRFKFQYISYDAGRTSSARKIFATVAGCVLAIGLVLIGG
                     IYFFKKKRNNTVKTEVNL"
     gene            complement(6276..>6906)
                     /locus_tag="PLI11_02140"
     CDS             complement(6276..>6906)
                     /locus_tag="PLI11_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPZ51695.1"
                     /translation="ANLSGFVLLFEIEPFIRLCSNEDMFLKLDGERIEKLKEELEDLD
                     FLQKCDYPGKEIMKISKTLYVLATLSNYISREQFRKLESPADSYTVAVMRCMEYIYQN
                     MGEKLYIDSLCSMFNMSRSCLFRSFKKICRMTPIQYQLKCRIDKARFLLEFTDNPIQD
                     IALECGFFDCSHFSKVFKNEVGDIPTKYRDKCRKSSTTDDHSYRMECFV"
BASE COUNT         1953 a         1633 c         1154 g         2166 t
ORIGIN      
        1 cttttacaaa ttgcatttac tacttccatt ttgcgtttac tttttcaatt aaccttttta
       61 tgtaaacaat tattttcttg tgtgtgaaaa agcggcacaa aaagcttttt gtaccgccta
      121 aacaatttac ctcagttctt caaaatccac tcccctgtaa tctgcgccgc ttgcggttat
      181 gatatcacag gattcaaaat atattatatc gcactcaggc atttcaaatg tatttttcat
      241 atattcacac tccatatagt ttatttccac cacataacaa cagactcgtc cagcatatcc
      301 tcgtgaccca cattgactat ttcctgatat acatctgtca cggacgcttt ctttacttca
      361 ctgtacgcat ataccgtttt accttctttc tcacacttaa tatacatacg cacgcagatt
      421 gccgtatcct tttcagcttc aggtatgttg taaacgtaag cagaattgta tatattacca
      481 ttatcttccc aaggcacaag ttctgattgc tttaccattt tcgcacctga cgccaagttt
      541 tcaaccaaca tctcaaaacc ttctgccata tttctctcaa gcattacgag tgcaccaact
      601 tcgcttacaa tatacacagt atttccaatc gttatattgc ttccaacctc cggtgcgtca
      661 aaacagaacc tcaaaccctg tctcaggtcg ctagtattgt agttatctcc atctttgaag
      721 ggtaccaatt tgctttttct gatttgatta cccgcaaaaa ctatttcctc tgcaatatat
      781 acggaagacg aaaggggagc gccatacatt ttttcaggaa ctcttgtgta cgcaattgta
      841 aactcgagtg atttccctgc atccaccttg ccatccttgt acatgcgctg accttcaaaa
      901 ttctcattta ttatccaaac aattgaatat ggcctttcag aatcagtatt atcttcagtg
      961 ttgtacacaa ccaacaaata ctctccgggt tcctgtttgc taaacttgaa caaagtatcc
     1021 ggatcatctg acgcacctcc aaaatggtta taatatgttg ctacaggttc accgcttacg
     1081 gttgttttgt agtctacatc ccatttgaac aatgcaaacc ttactgactt gactttccct
     1141 ccccatgttg gagtattaat tattattgag ttaaatggtg ctgttgcgat aaactgtgat
     1201 gctgcattat tctccacaaa atatgcttta tgcacatctt tggggtttga agccaaataa
     1261 aaatcatagt taataccact ttcagactca ctaacttcgc caaaatatgt atccgaaaca
     1321 tctgtaaagt taagtttcat actaagagta acatcttgct ccatacccgc taaaaatact
     1381 ttatggtatg acttaacagg agttgtaatt ttatagatat aggatgttga acctttcgtc
     1441 gaagaaaaga ccattaaata ctctcctgat tcagcagtat aaacatttcc gccatcgtcc
     1501 aagaaagtaa aattgacgaa actgtcattt ggtgtcgcag gtatattagc ggttgccaaa
     1561 acttccccgt caatggttgt cgcataatcc ccatcccatt tatatagttc aacagcatga
     1621 gatacctctc caccccaagt gccgaacttg gcattccctc ctgtaaaggg caccagtgca
     1681 ttcacttgga cagcatgaat ttcagccgag accttctctt ggtaagctcc accctccata
     1741 tcaaacattt cctgcaaatt cgaggcagcc actacttttc caggttcctc aatgaatatc
     1801 aatccacaaa taatcactac acataacaag ctaagaatta gtatgatttt cttcattata
     1861 taacctccat tcaatattta tattcactac gacttaattt cccttagtac aagactaaca
     1921 acaaccatga gattataatt gtattattat ctcagatttt tgtaatatag tttctcttga
     1981 tacattaaaa gaatcccaat agatataaca ggattctttt agacatcagt ctttcaggat
     2041 aaactttcct ttgtcaaaaa gaagctcacg ccgggaaaga taccaacttt tacccatgtc
     2101 aggtgaacct tgataatata accacaaatg accatcgtcc gtctcataaa catatggatg
     2161 tcccgactca cttgcattcc agtcaccagg cgctccattt gacaaaaatg gttggtcgga
     2221 aagccttcta aagcggaagc cgtcgtcaga tacagcacag ccgatttgct gaggggaaca
     2281 gttgtatgct cctgcataaa acatatatac ttttccatct attacaacgg atgcaggacc
     2341 ctctatacaa gtcatttccc aatccagctc tggctttagt accggctctt tatgcaaata
     2401 gaaatcccct gcactgaaat cggaatccaa gtaagcccag gctgctccga cgatctgctt
     2461 ttcaaagtcc ttatcgcgag tagcaaaata gagtatcatt ttatctccga aaacgcagac
     2521 atccgcatcg atagccctcc cgcacgacca ggaaggcatt tttgcccaag gcattcgggg
     2581 agcaaaaaca ggatttgatt catctttaac aaaattaatt ccatctgaag agacggcatg
     2641 gcatatggca tcaagcttgt tattgccata ggtctggtaa aataggtgta tttgtccttt
     2701 tactaggatg catccgggcg caccaatacc atttttctca catttttgtg ttattggcaa
     2761 attagcgata cgtacaaagt tttctccatc ctccgagcat gagattccga tgttgagtct
     2821 tccatcaatc ataatcgaat gatagaggaa gtacttcccg ttaaattgta caacagcagg
     2881 atcttttgca tagttttcaa aatccgcatt tgtgtataac atgatatcaa tacgcctcct
     2941 tttatacttc gcttttattc aagacctgaa gcctgaatcg gaccatgaat atttgaaggc
     3001 tttttggtgt aataaacaac gagtcttggt gtgcccgctt cccatatttc attattgtaa
     3061 tacgacctta cataatcaac tgtacctgct ttataccaaa cgcagacatt gtccgtatcc
     3121 tcgttgtaga tttcatatag atattctcca tcaggcagtt cttcgtccag ctcaataaga
     3181 acttccacgc catctttcca gtctgccact tcctttgtta caactggctc ggacatcatg
     3241 gtagcttcgt aagatccttg ccatgcataa agcgaaaggg tggccgcatg gccttcctcc
     3301 tgaaaccagg taggcatact gattcctact ttgttgaatg tagtggtagc aaagaattgc
     3361 acagaaaacg aaccaaaaat cttatgagcc tgttgaccaa catgaccacc ttcatagaaa
     3421 tctgctttta tagccttagt ctcatctatt tccccagcat actggtttgt cccaggttca
     3481 actgtaggca agggtgtcgc agtttcagca ggagtcgctt cctctgttgg tgcgggagtg
     3541 tctgtaggtt cgactgttgt attagatgtt gcttcagggg tagggctagg cgtcgcttgg
     3601 tgttctgact cttttgcgca acccacaaaa accaacaatg tcaatgcaag aattgaaaaa
     3661 aataattttc tcataaattt acctccgttt taactgtatt gtttctcttc tttttaaaga
     3721 aatatattcc accgattagc acaagtccta ttgcaagcac gcaccctgca acggtggcaa
     3781 agatttttct tgcagaggaa gtcctgccag catcataact tatgtactgg aacttgaatc
     3841 ttccgccagg agcagcatct ccgtttacat aaaaatccat cacatcgcca tcattctgca
     3901 tgttgtcaga ccacttaaag ttaacaacaa atttgtcacc attgatgcca agcattgatt
     3961 taggtacctt aatctgaagt ctgttgtctt taacagagta ctctacatct ccgactttct
     4021 cccaattcca tccaccgttt gacttctcaa ggactgcttt ctcaccagga ctaaccctgt
     4081 taataatgta ctcaaaagtc tcccagttcg gacctttttg tccctccacg tctataaaca
     4141 acctcatcca tgccggatcg gtgctggacg aaatattctc tttcgtctca accataaaat
     4201 aaacaaaatc gttgtcatga gtaactttgg cccctactat gtcgtttctt cctgatgtgt
     4261 tctcataatg ataacctaaa tatcccgggc tgtttctgtg caaggtattg ccaccatagg
     4321 aagcaaaata cggttttaca tctgtccaca tgtcctcttc ggaatatatg tctattgtct
     4381 tgcctttaga tgctgcctct ggcttctcta cgcccttgaa ccttctaaca aaggaaacca
     4441 gttggtaata gtagtgatcc ttaagttcac cttttgaagg ctcaatatca cgcgagtagg
     4501 catcgttgaa ctgatcaggg aaggcattcg gcacccccat ccactcttca tatcttcccg
     4561 ctctccattc attccatccg gtaatgaata taaattcggg atccacttcg agagcgtatc
     4621 tgaattgttc ttcaaagttt gctccataaa gaatggcgtt ttctcttgag tcatattctt
     4681 tcttcgcatt catccagtgt cttccgaaca ccctctcgcc attcattgcc acaagccctt
     4741 gcttgtcgtt atggttctgt gcaacactta cagttatctg ctccggtgta ccatcctcgt
     4801 tgtagtatac ctgttgggga taaattgagc accaacccca ctggtctggt cttgtctgtc
     4861 catcgacata tgaaggctgt ggtggcctga aattaaagaa ctcaagtatt tcagcctcaa
     4921 gctcgtcatt tggatttaac ttgtcaggat aagccatcat gagaggtttt cctttccagt
     4981 aaaaccaaat atccttgtac ttgcccggtc tgtagatttc ggtatacaac tctctgagct
     5041 gaacaacggt gttgggccag gtgccctcat agaaattcat gaggaatgag atctggggag
     5101 tcttcactcc atcagctctt gcctgtgcaa aagtctccag aagcaccttt actccatcga
     5161 tatatgtggc tgttctattg ctgttatcga agataatgac atctacacct gcatcagcaa
     5221 gcaactcggc ctgtttccta aaaacccaag gatcttttcc attgtaatat ccgtatattg
     5281 gctcattcca gaaatacgag tcatgcaccc caccccattc ttcggcactg taatcataaa
     5341 gaatatccgg gtgtttttta agaatctctg taacatttct aggaaccgag gcagaatact
     5401 tttcatgcca tgtccagaaa aacatgccta catactttcc atctcttaaa ggaccaactt
     5461 cattataatc aggcagcgtc ctgtcaagac cgtcagtagc aacatatgaa cctggtcttg
     5521 caagccctat taaacttgtc tcatcagttt tagaaagagc agccaaattg tttttgggtg
     5581 tatgtatata attcaatctt gcctgaagag aacctgccat gtcacttccg ttaaaataca
     5641 gtgctacatt ttccttgtca gacggtattt ttgcaaggga tacgtttcct gtgaaattac
     5701 ataaaacagc caggtactca cccgaaccta ccgtactgtc aaattcaaac tttgcccaat
     5761 tgttccttgc aaactttgat atttgctgag taactactgc gtcacttgcc gacgtcttct
     5821 tgtagctgcc cttccattca taaagaaaaa gctgcatgct gtttctttga ataagggcat
     5881 ttgtaacaaa aaactccaca ccatcatatt ctgttgtagc atgaaacctt atgcccaggg
     5941 tttcgtttgc ggccatgggc aggtttacaa ccttgtctcc ttcgttatac aagacagcag
     6001 aagtgcgctg tccctcaata agacctccgt gcatctcttc tccttccttt cctgcgatga
     6061 ctgtaagcaa tacagctagt actagactta tacataataa aaaaaacttt ctagaagaca
     6121 tcttttcact ccctagtatt tattcaattg gatatatatc gatccataaa tacttttaca
     6181 cattatattt ctactgaaca gaactaccta gttcagaata acaacaattt tgaaataata
     6241 attgtattat tatcgcaaat atttgtataa tagtttcaaa cgaagcattc cattcgataa
     6301 ctgtgatcat cagtggttga agattttcta catttatccc tgtattttgt tggtatatcg
     6361 ccaacttcat ttttgaacac ttttgaaaaa tgagaacagt caaagaaacc gcattcaagg
     6421 gctatgtctt gtattggatt atcggtgaat tcaagcagaa atcttgcctt gtcaattctg
     6481 cattttagtt gatactgaat gggagtcatc cggcatattt ttttaaaact tctgaaaaga
     6541 caggatctgg acatattgaa catgctgcaa aggctatcta tgtacaattt ctcacccata
     6601 ttctgatata tgtattccat gcacctcata actgccaccg tatacgaatc ggcgggcgac
     6661 tcaagtttgc gaaattgctc acgggaaatg taatttgaca gtgtggcaag aacatacaaa
     6721 gtcttggata ttttcattat ttcctttcca ggataatcac atttctgcaa aaaatccaaa
     6781 tcttccaatt cctctttgag cttttctatt ctttccccat caagtttcaa aaacatatcc
     6841 tcattagagc acaaccttat aaacggctca atttcaaaaa ggagcacaaa accagataag
     6901 ttggcc
//
LOCUS       DAONVY010000047         6776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8471_length_6776_cov_3.719685, whole genome shotgun sequence.
ACCESSION   DAONVY010000047 DAONVY010000000
VERSION     DAONVY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_6776_cov_3.719685"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PLI11_02145"
     CDS             complement(<1..1300)
                     /locus_tag="PLI11_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018976569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPZ51696.1"
                     /translation="MRFRQVHLDFHTSEKIAGIGSKFSKEQFQKALKMGHVDSITLFA
                     KCHHGWAYFPSEANERHPCLDFDLLGSQIEAAHEIGVKTPVYISAGLDEKLARRRPEW
                     LTRPENDQLNWKDAGYHEFCMNSPYIDYFMPQVEEVVKKYDVDGVFLDIVGVRICYCQ
                     NCINTLLERGKEPYVYENHLELGEEVYANYLKRAREAVDKYKKGLPLFHNSGHIRRGR
                     RDLAYANTHLELESLPTGGWGYDHFPLSSAYARTLNMEYLGMTGKFHTTWGEFGGFKH
                     PNALLYETALSLANGAKCSIGDQLHPLGFMDEATYKLVGIAYKEVEKKEEWCDNVESV
                     CDIAILSEEAIQNYYKTPMDSSGDVGAVRIMLEGKYLFDVVDQEASLDKYKLLILPDS
                     IRVDEYLKEKIDNFIQNGGKILATGLSALYKDRDEFAFDLG"
     gene            1424..2368
                     /locus_tag="PLI11_02150"
     CDS             1424..2368
                     /locus_tag="PLI11_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51697.1"
                     /translation="MRTEYWAFLDKYNVADIRNNNEDKQTRKKLEEILTDNYKQIKSN
                     ILNLPHRIDNLVFIRARFLKYRRIALFVFTLFVLVVSAFVYLFRNWPGVSLFYYRMYL
                     ISVFTILMMMLFPALVVYVNKVRGEMIRFVRYIIIHGEEELELPIPDYLRKIFVPFGK
                     GKEKVVLGKIRCGCANEGNMRLVANTTYVNEGLPVNSYTNVTYLKAHCLDCGTEHVLF
                     DVNKHGWNGFVRQKQEGEHASFSFVECPLCRCLSHSVHMTLHSPGKEMFVRESFLLRS
                     DGSSMRESDWVNAFSHISIFSICSNCRKRFLILDKETM"
     gene            2415..2906
                     /locus_tag="PLI11_02155"
     CDS             2415..2906
                     /locus_tag="PLI11_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51698.1"
                     /translation="MKFDIDEIRHGIRNGEFKNIGNGTGRSVYDIGEGIVAKNAKNIG
                     GIAQNFTEHAIYSNRCSNIFAKVYTLSHDGEILLMEKAKPLEDMDKLLEHFNACCRKH
                     FASTQLVKYLSRKYKLLPADLCKPGSWGETENGLVLIDYGFTKWVSRNFYYDFSRKYS
                     LLC"
     gene            2955..3833
                     /locus_tag="PLI11_02160"
     CDS             2955..3833
                     /locus_tag="PLI11_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51699.1"
                     /translation="MKRALFLMLALSMVLVFVSCNGGGGEPKATEDPKETKSSATPTP
                     DPTASPTPEPTEDPTKTSEPPEGYDETVEVPKADIFDALIKDGTVTDQSENSLTVTNV
                     NEAVVTKDDSIGKDVIDLYSEDRAFFAVFNFDTEYDKLADGFTFETSVNLNSPNAYQV
                     VGGNQQAGGFCIDYQPDAANGNGALGFGVHNGTEYIKIYPEKRSEFDRYYHVVGVFDG
                     PNVKLYINGRLIGQEELGNDMKFPPDIKYLAIGADTGVDDTGEYFLDGKISIFRLYND
                     SLNDSQIYKLYLQSLE"
     gene            3866..4360
                     /locus_tag="PLI11_02165"
     CDS             3866..4360
                     /locus_tag="PLI11_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPZ51700.1"
                     /translation="MFKRRSIRRYRDMPVTEEQLEEILRAAMAAPSAGNQQPWHFIVI
                     NDRSILKEIPKFHPYANMLNEAPCAIVVCGDTSRERHKSYWVQDCSAATQNMLLMAAE
                     MGLGSVWLGVYLREERVSELRKLLDIPDNIIPLNIVAIGHPAEEKGPSDRFDETRIHK
                     NKWR"
     gene            complement(4357..5781)
                     /gene="hydG"
                     /locus_tag="PLI11_02170"
     CDS             complement(4357..5781)
                     /gene="hydG"
                     /locus_tag="PLI11_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPZ51701.1"
                     /translation="MYNVKSKDSNEFINHDEVLFSIEEAKKKSNDKNYILTLVEKARQ
                     YKGLTHREAAVLLEVDDDEILEMMYKAAKEVKEKIYGKRIVIFAPLYLSNYCVNNCRY
                     CGYKCTNDIARYKLTQEELVEEVKALQELGHKRLLLEAGEDDKNCPIEYILECIKTIY
                     SVKHNGGEIRRVNIDIAATTVENYRKLKEAGIGTYLLFQETYHKPTYISMHKGPKQNY
                     EWHTEAMDRAMQAGIDDVGIGPLYGLYDYKYETVALLMHAEHLEAVYGVGPHTISIPR
                     LLPAAGVDYNSFPHLVNNRDFKKIVATIRLAVPYTGIILSTRESAEFRKELLEVGVSQ
                     MSAGSSVGVGGYAQRKKMQEMGLDEKPQFEISDHRAPIEVIKGLVKDGYLPSYCTACY
                     REGRTGDRFMALAKTGQIANCCLPNALLTFEEYINDYGDDELRQLGSEMIDKEIQTVP
                     NQRQRERAVDYLGRIRTGERDLRF"
     gene            complement(5792..>6776)
                     /gene="hydF"
                     /locus_tag="PLI11_02175"
     CDS             complement(5792..>6776)
                     /gene="hydF"
                     /locus_tag="PLI11_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPZ51702.1"
                     /translation="FGDLGELRVNKTKDAAAKTDIALIVIGDDVTNEDLNWYNYFKDK
                     KIPAIVVLNKMDLYDRDVLKDKIKKIKEAFDTNPIPASCKDKKGINTLLNDIARNLPE
                     DFNARTITHNLAKEGDVVLLVMPQDIQAPKGRLILPQVQTIRELLDKKCVVVSCTVDN
                     LKQTLDTLKNPPSLIITDSQVFKTVYELTPKESKLTSFSVLFAGYKGDIKYFAESAAL
                     LDSLNENAKILIAEACTHVPMEEDIGRVKIPSMLRKKFGDKITITNVAGCDFPEDLET
                     YDLIIHCGACMFNRRYVLQRIENAKQKKVPMTNYGIVIAYLTGILDKISME"
BASE COUNT         1858 a         1357 c         1434 g         2127 t
ORIGIN      
        1 cacccaaatc aaaggcaaat tcatctctgt ccttatacaa agcagacaaa ccagtagcaa
       61 ggattttgcc tccgttttga atgaagttgt ctatcttttc ttttagatac tcatcaactc
      121 taatactgtc aggcaaaatc agaagcttgt atttgtcaag acttgcttcc tgatctacaa
      181 catcaaacag gtacttgcct tcaagcatta tcctgacagc acccacatct cctgaagaat
      241 ccatgggtgt tttgtaatag ttttgaattg cttcctctga aaggattgca atatcgcaaa
      301 cactttccac attgtcgcac cactcttcct ttttctcaac ttccttgtat gcaattccta
      361 caagtttgta tgtggcttca tccatgaatc ccaaaggatg taattggtcg ccaatggagc
      421 attttgcacc attggcaagg ctcaaggctg tttcgtaaag aagtgcgttt ggatgtttaa
      481 aacctccgaa ttcaccccag gtggtgtgga atttgcccgt catgccaagg tattccatgt
      541 ttagggtgcg ggcataggcg ctggatagag ggaaatggtc gtaaccccat ccgcctgtag
      601 gaagactttc cagttccaaa tgtgtgtttg cataagcaag atcccttctg cctctcctta
      661 tatgaccgct gttgtggaaa agaggcagtc ctttcttgta tttatccaca gcttctcttg
      721 ctcttttcag atagtttgca taaacttcct cgccaagttc caagtggttt tcgtaaacat
      781 agggttcttt gcctctttca agaagtgtat tgatgcagtt ctggcagtag catattctta
      841 ctcccacaat gtccaaaaaa accccgtcca catcgtattt ttttactact tcctctacct
      901 ggggcatgaa gtagtcgata tatggggagt tcatacaaaa ctcatgatag cctgcatctt
      961 tccagtttaa ctggtcgttt tccggccttg taagccattc aggtcttctt cttgcaagtt
     1021 tttcatccag ccctgcggat atgtacacgg gtgtttttac ccctatttcg tgggctgctt
     1081 ctatctggct gccaagaagg tcaaagtcaa gacagggatg tctttcattg gcctctgacg
     1141 gaaaatacgc ccacccatgg tggcattttg caaacaaggt tattgagtca acatgtccca
     1201 ttttcaatgc tttttgaaac tgttcttttg aaaacttgct cccgattcct gcgatttttt
     1261 cgctggtgtg aaaatccagg tgtacttgtc taaatctcat gtttgtccct cttttcatct
     1321 tagttaatac aattttataa tttttccaca accagtcaac ccaaaacgga cagattgtga
     1381 tagaataaag atataaatta atagtagtta atcgaggttg gacatgagaa cagaatattg
     1441 ggcgtttttg gataaatata atgtagcaga tattagaaac aacaatgagg acaaacaaac
     1501 aagaaaaaag cttgaagaaa tactaacaga caactacaag cagattaaat cgaatatttt
     1561 gaatcttccc cacaggattg acaatcttgt attcataagg gcaagatttt taaaatacag
     1621 gaggattgcc ctgtttgttt ttactctctt tgttttggtg gtttcggcat ttgtgtacct
     1681 attcagaaac tggccgggtg ttagtctttt ttactataga atgtatttaa tatctgtctt
     1741 tacaattcta atgatgatgt tgttccctgc tttggttgtg tatgtgaaca aggtaagggg
     1801 agagatgatt agattcgtca ggtacataat tattcatgga gaggaagaac ttgaattgcc
     1861 aatacctgat tacctaagaa aaatatttgt gccttttgga aaaggaaagg aaaaggtggt
     1921 attggggaaa ataaggtgtg gatgtgcaaa cgaaggtaat atgaggcttg tggcaaacac
     1981 tacctatgta aacgaaggtt tacctgtaaa cagttatact aatgttacat atctaaaagc
     2041 tcactgcctt gactgcggta cggaacatgt tttgtttgat gtaaacaagc atggctggaa
     2101 tgggtttgta aggcaaaagc aggaggggga acatgcttcc ttttcctttg tcgagtgccc
     2161 tttatgcaga tgtctttccc attcagtgca catgaccctt cactctcctg gaaaagagat
     2221 gtttgtaagg gaaagctttc ttttgaggag tgacggttca tctatgagag aaagcgactg
     2281 ggttaacgca tttagccata taagcatatt ttcaatatgt tcaaactgcc gtaagaggtt
     2341 tttgattcta gataaagaaa cgatgtaaca ctcttaatca ccctgaatat atcatgatat
     2401 tagggtgatt tttgttgaaa ttcgatattg atgagataag acatggaata agaaatggag
     2461 aatttaagaa tatagggaac ggaacaggta gaagtgttta tgatataggg gaggggatag
     2521 tggcaaagaa tgcgaaaaat ataggaggga ttgcccagaa ctttacggaa catgccattt
     2581 actccaatag atgcagcaac atattcgcaa aagtttacac cctttcccat gatggagaaa
     2641 tactgctgat ggaaaaagcc aaaccattgg aagatatgga taaattgctc gaacatttca
     2701 atgcttgttg tagaaaacat tttgcatcta cccaacttgt taagtatttg agcaggaagt
     2761 acaagctgtt gcctgcggat ctatgcaaac cgggaagctg gggagaaact gaaaatggtt
     2821 tggttttgat agattatggc ttcacaaaat gggtaagtcg caacttctat tacgattttt
     2881 ctcgaaagta ttctttactt tgctaatttt tttggtaata tataggcaag aatatcaggg
     2941 aggtaaagta gaatatgaaa agagctttat ttcttatgct tgccttaagt atggttcttg
     3001 tctttgtgtc ctgcaatggc ggagggggag aacccaaggc tacagaggat ccaaaggaaa
     3061 caaaatcttc tgctacacca actccggatc caacggcttc accaacaccc gagcccaccg
     3121 aggatccgac aaagaccagc gagccgccgg agggttatga cgaaactgta gaagtgccaa
     3181 aggcggatat ctttgatgcc cttattaaag acggcactgt aactgaccag tcggagaaca
     3241 gtcttacagt gacgaatgta aatgaagctg tagtgacgaa ggacgattcc attggcaaag
     3301 atgttatcga cctttacagt gaagacagag ccttctttgc agtgttcaat ttcgatacgg
     3361 aatatgacaa gcttgcagat ggttttactt ttgagacaag tgttaatctc aacagtccaa
     3421 atgcatatca ggtggttggt ggaaaccagc aggctggagg attctgtatt gattatcagc
     3481 ctgacgctgc aaatggcaat ggggcacttg gctttggagt acataacggt actgaataca
     3541 taaagattta tcctgaaaaa agatccgagt ttgacagata ctatcatgtt gttggtgtgt
     3601 ttgatggacc taatgtaaaa ttgtacataa acggaaggct tataggtcaa gaagaacttg
     3661 gcaacgacat gaaattccct cctgatatca agtatcttgc aattggcgca gatacaggag
     3721 tggatgacac tggtgagtac ttcttggatg gcaagatttc catattcaga ctttataatg
     3781 attcgttgaa tgactcgcaa atctacaagc tgtatttgca gtcgttggaa tagtgaagaa
     3841 caaggagggt ttttagagga atgccatatt caaaagaaga agtattagaa ggtatcggga
     3901 catgccagta actgaagaac agctggaaga aatattaaga gctgcgatgg cagctccctc
     3961 tgcggggaac cagcagccct ggcattttat agttatcaat gacagaagca ttcttaagga
     4021 aattccaaag ttccatcctt atgcaaacat gcttaacgaa gctccctgtg caattgttgt
     4081 atgtggagac acatctcgtg aaagacataa gtcatattgg gtgcaggact gctcggctgc
     4141 aacacagaac atgcttctga tggctgcaga aatgggtctt ggttctgtct ggcttggtgt
     4201 gtatctacga gaggaaagag tcagcgagct cagaaagctt cttgatatac ccgataacat
     4261 cattcctcta aacattgttg caataggtca tcctgccgag gaaaagggac caagtgacag
     4321 gtttgatgaa acaaggatac acaaaaacaa gtggcgttaa aaccttaaat ccctttcccc
     4381 agtccttatc ctgccaagat aatcaacagc gcgctctctt tgcctttgat tcggaactgt
     4441 ttgtatttcc ttgtctatca tttctgagcc taattgtcta agctcatcat cgccatagtc
     4501 gtttatgtat tcctcgaagg taaggagagc gtttggaaga cagcagtttg caatctgccc
     4561 ggtttttgca agggccataa atctgtctcc ggttctacct tctcggtagc aagctgtaca
     4621 gtagctgggt agatatccgt cttttacaag tcctttaatt acttcaatag gagctctgtg
     4681 gtccgaaatt tcaaactggg gtttttcatc aagacccatc tcctgcattt tcttcctttg
     4741 tgcgtaacca ccaacgccaa cgctggaacc tgcactcatt tgcgaaacac ccacttcaag
     4801 aagttccttt ctgaactccg cactctccct ggtggatagg attataccgg tgtagggtac
     4861 agcaaggcgt attgtagcta caatcttttt gaagtccctg ttgtttacaa gatgtgggaa
     4921 actgttgtag tcaacgcctg ctgcaggaag aagtcttggg atggatatgg tatggggacc
     4981 tacaccatag actgcttcca aatgctctgc atgcatgaga agcgccaccg tctcgtattt
     5041 atagtcgtac aatccataca aaggaccaat gcccacatca tcaattcctg cctgcattgc
     5101 cctgtccatt gcctcggtgt gccattcata gttttgcttt ggacctttgt gcattgatat
     5161 gtatgtgggt ttgtggtatg tttcctgaaa caggaggtat gtaccgatgc ccgcttcttt
     5221 tagttttctg taattctcta cagttgtggc ggcgatatcg atgtttaccc tgcgtatttc
     5281 tccgccgttg tgctttacac tgtagatagt ctttatgcac tcaagaatgt attctatggg
     5341 gcagtttttg tcgtcttctc ctgcttccag aagcagtctt ttgtgtccca attcctgaag
     5401 tgctttcact tcctcaacaa gttcttcctg ggttaattta taccttgcaa tatcgtttgt
     5461 acacttgtag ccgcagtacc tgcagttgtt gacgcagtag ttggacaggt acaaaggagc
     5521 aaagataaca atccgttttc cgtagatttt ttcctttact tcctttgcgg ccttgtacat
     5581 catttccaat atctcgtcat catcaacttc caaaagaacg gcagcttctc tatgtgtaag
     5641 acctttatac tgccttgctt tttcaaccag ggtaagaata tagtttttgt cattggattt
     5701 ctttttggct tcctcaatgg agaaaagaac ctcatcgtga ttgataaatt catttgaatc
     5761 ttttgatttt acgttataca taacagtacc tctattccat ggatattttg tctagaattc
     5821 ccgtcaggta ggcaataact atgccgtaat tggtcatggg aacctttttt tgttttgcat
     5881 tttctattct ctggagcacg tatctcctat tgaacatgca tgcaccgcag tggattatca
     5941 aatcataagt ttccagatct tctgggaaat cacatcctgc aacgtttgta atggttatct
     6001 tgtcaccaaa cttcttcctc agcatgcttg gtatttttac tcttccgatg tcctcttcca
     6061 taggaacatg ggtgcatgct tctgctataa ggatttttgc attttcattc aaactgtcga
     6121 gtaatgcggc gctctctgcg aaatatttaa tatcaccctt gtagcctgca aagaggacgg
     6181 agaaacttgt aagtttgctt tcctttggtg taagttcgta tacggttttg aatacctgtg
     6241 aatcagtaat tattagggaa ggaggattct tcaaagtgtc aagggtctgc tttaggttgt
     6301 caaccgtgca acttacaact acgcatttct tatccaaaag ctccctgatg gtttgaacct
     6361 ggggcagaat cagtcttcct tttggtgcct gtatatcctg gggcattacc aaaaggacca
     6421 catccccctc ctttgccagg ttgtgggtta ttgttcttgc attgaaatct tctggaagat
     6481 ttcttgcaat atcgtttaaa agtgtgttaa tccctttttt atccttgcag cttgccggta
     6541 tgggatttgt atcaaaggct tcctttattt tttttatttt atctttaagc acatctctgt
     6601 cataaagatc catcttgttt aatactacaa ttgcgggtat tttcttatcc ttgaagtagt
     6661 tataccagtt caaatcctcg tttgtaacat catcaccaat tacaatcaag gcaatgtctg
     6721 tttttgcagc tgcatcttta gttttattta ccctgagctc acccaaatca ccaaaa
//
LOCUS       DAONVY010000048         6737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8523_length_6737_cov_3.774768, whole genome shotgun sequence.
ACCESSION   DAONVY010000048 DAONVY010000000
VERSION     DAONVY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8523_length_6737_cov_3.774768"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLI11_02180"
     CDS             <1..510
                     /locus_tag="PLI11_02180"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPZ51703.1"
                     /translation="ILVTTSMYVAEKVREKVNERLKTLERNTIAKKSIENNGAIIVVD
                     NLDEAAFLSNSIAPEHLELILNDKILTDEFIDKFYNAGAIFLGEYSPEPMGDYFCGSN
                     HVLPTGGTAAFSSPLGVWDFVKRTSIVNYTKEGFFKDCEHAYNMAMAEGLTAHAKSLK
                     ARCDDEQVF"
     gene            494..1552
                     /gene="hisC"
                     /locus_tag="PLI11_02185"
     CDS             494..1552
                     /gene="hisC"
                     /locus_tag="PLI11_02185"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPZ51704.1"
                     /translation="MSKYFNKRTKNLIPYVPGEQPKEGETFIKINTNECPYPPSPRVI
                     DAICQSVNESLRLYPAPDASLARRAFADKYNLSLECVFAGNGSDEVLAFSFLAFFAKD
                     KKVLIPDITYSFYTVYCDLCELECKQIPLKDDFTIDVDAFVREKGNVVIANPNAPTSL
                     ALDIGDIERILNAHPDDVVIIDEAYVDFGGESCIGLVNKYDNLLVVQTLSKSRALAGM
                     RVGFACGSKELVDGLNRIKDSFNSYTLDRLAIVAAEAALKDDEYYKSVIDKIVSTRES
                     VKKELMELGFKVLDSRTNFLFISHKTKNAEDIMSYLRSKGVLVRHFKTNRIDNYLRVT
                     IGTDEEMDKFLSLIKDME"
     gene            1561..2145
                     /gene="hisB"
                     /locus_tag="PLI11_02190"
     CDS             1561..2145
                     /gene="hisB"
                     /locus_tag="PLI11_02190"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPZ51705.1"
                     /translation="MDRKAFVKRETRETKIEASINLDGGAYDINTGIGFFDHMLELFA
                     KHSGIGLSVEAKGDLYVDCHHTVEDVGIVVGTLLKEAAGSKDGIKRYGHSVIPMDEAL
                     SEVVIDFGGRSFFILKGEIPCIRLGEFETETVQEFFTSIASNAGMNLHVIVRYGTNTH
                     HIIESVFKAFAHALKQAISYDPNQKGVLSTKGVL"
     gene            2147..2808
                     /gene="hisIE"
                     /locus_tag="PLI11_02195"
                     /pseudo
     CDS             2147..2808
                     /gene="hisIE"
                     /locus_tag="PLI11_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
     gene            2951..3235
                     /gene="groES"
                     /locus_tag="PLI11_02200"
     CDS             2951..3235
                     /gene="groES"
                     /locus_tag="PLI11_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPZ51706.1"
                     /translation="MKIKPLADRVVIKMLEQEETTKSGIVLPGSAQEKPQLAEVVAVG
                     PGAVVDGKKVPMEVKVGDKVIMSKYAGTEVKFDGVEYTILKQSDILAIVE"
     gene            3261..4883
                     /gene="groL"
                     /locus_tag="PLI11_02205"
     CDS             3261..4883
                     /gene="groL"
                     /locus_tag="PLI11_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814034.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPZ51707.1"
                     /translation="MAKEIMFGEESRKALLNGVNKLADTVKITLGPKGRNVVLDKKFG
                     SPLITNDGVTIAKEIEFEDRYENMGAQLVKEVATKTNDVAGDGTTTATLLAQAMIREG
                     MKNIAAGANPMILKRGIAKAVEVAVESIKENSQKVRGREDIARVASISANDEVIGNLI
                     ADAMEKVTNEGVITVEESKTMGTSLEVVEGMQFDRGYVSAYMATDTDKMEAVLDDPYI
                     LITDKKISNIQELLPVLEQIVQHGKKLLIIAEDVEGEALATLVLNKLRGTFTCVAVKA
                     PGFGDRRKEMLRDIAILTGGEVISEELGLELKDTTIDQLGRARQVKVAKENTIIVDGA
                     GDSDDIQKRIASIKAQIEETTSEFDKEKLMERLAKLAGGVAVIQVGAASEVEMKEKKL
                     RIEDALSATRAAVEEGIVPGGGTAYINAISKVTELLDKTEGDEKTGIMIVLKALEEPL
                     KQIAINAGLEGAIVVDRVMKEDKGVGFDINSEEYVDMISHGIVDPAKVTRSALQNAAS
                     VAAILLTTEAAVSDIPEPEPAVPAGGMGNGMY"
     gene            4975..5490
                     /locus_tag="PLI11_02210"
     CDS             4975..5490
                     /locus_tag="PLI11_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51708.1"
                     /translation="MEIIIKRETDSFYTFLSFMIMVAAVVLLVLLYNFAGYIVFVPTP
                     FILFAAGIVSRLVYINYNKVEFEYSLHSGIFNIDKLVNQAKRQPVIKLPASDILTFGR
                     YNDSVDEEYKDGTKHVDCSSGRDDVDLYYFTCHQNQGRFLIIFEPNEKLLAMLKGYSS
                     VVAKALLKDNK"
     gene            5570..>6737
                     /locus_tag="PLI11_02215"
     CDS             5570..>6737
                     /locus_tag="PLI11_02215"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPZ51709.1"
                     /translation="MAYIYDEPSHTFSEYLLLPGLATKQCIPSNVSLRTPVVKYKEGE
                     ESEIYMNIPLVSAIMQSVSDDKMAIALAKCGGLAFIYGSQSIEEQAEMVKRVKKYKSG
                     FVVSDSNLTPENTLEDVLNLKKEKGHSTIAITHDGSPNGYLMGIVTSRDYRVSRMSPE
                     TKIKDFMTPFEKLVFAREGITLKQANDIIWDHKLNTLPIIDEENRLRYLVFRKDYDQH
                     KENPVELLDKEKRYMTGAGINTRDYRERVPELVNAGVDVLCIDSSVGYSEWQKDTLEF
                     IKTEYNGKVRCGAGNVVDAESFLYLAEAGADFIKIGIGGGSICITREQKGIGRGQASA
                     VIEVAKARDEYFKKTGIYIPLCSDGGIVHDYHITLALAMGADFVMLGRYFARFDE"
BASE COUNT         2147 a         1084 c         1638 g         1868 t
ORIGIN      
        1 atacttgtaa ctacaagtat gtatgtagct gagaaggtta gagaaaaggt caatgaaaga
       61 cttaaaactt tggaaagaaa cactattgcg aaaaagtcta ttgaaaacaa tggcgcaata
      121 attgttgttg acaatttgga tgaagcagct tttttgtcta attcgattgc acctgaacat
      181 cttgagctta tactaaacga caagattctt acggatgaat ttatagacaa gttttacaat
      241 gcaggagcaa tatttttagg cgagtattca ccggaaccca tgggcgacta tttctgcggc
      301 tcaaatcacg tattgccaac aggaggaacg gctgcatttt catccccttt gggagtatgg
      361 gattttgtaa agagaaccag tattgtaaac tatacgaaag agggcttttt caaggattgc
      421 gagcatgcat acaatatggc gatggccgaa gggcttacag ctcatgcaaa aagtttgaag
      481 gcaaggtgtg atgatgagca agtattttaa caaacgcacg aaaaatctaa taccgtatgt
      541 acctggtgaa caaccaaagg aaggcgagac ttttattaaa atcaatacga atgaatgccc
      601 atatccgcca tctcccagag taattgatgc aatttgtcaa agtgtcaatg agtctttaag
      661 gttgtatcct gcgcctgatg caagcttggc aagaagggct tttgcggaca agtataatct
      721 gtctcttgag tgtgtgtttg caggaaacgg ctcggatgaa gttttggctt tttcttttct
      781 tgcgtttttt gccaaggaca aaaaagtact tatacctgac attacctaca gtttttatac
      841 cgtatactgt gatttgtgcg aacttgaatg caaacagatt cctttaaaag atgactttac
      901 aatagatgtg gatgcttttg ttagggaaaa gggcaacgtt gtcatagcaa atccgaatgc
      961 tccaacatct cttgcgcttg atattggtga tattgaaaga attctaaatg ctcatccgga
     1021 tgatgtggta atcatagatg aggcgtatgt ggattttggt ggagaaagct gtattgggct
     1081 ggtaaataag tatgacaatc ttcttgttgt gcagacattg tcaaagtcaa gggctcttgc
     1141 aggcatgaga gttggttttg cttgtggcag caaggagctg gttgacggat taaacaggat
     1201 aaaggattct tttaattcat atacactgga caggctcgca atagttgcag ctgaagctgc
     1261 attaaaggat gatgagtatt acaaatctgt cattgataag attgtatcaa caagagagag
     1321 tgtgaaaaaa gaacttatgg aacttggatt caaggtgctt gattccagga caaactttct
     1381 gtttatttcc cataaaacaa aaaatgcgga agacattatg agctatttaa gatcaaaggg
     1441 tgttttggtt cgtcatttca aaacgaatag aattgacaat tatctaagag tcaccatagg
     1501 aactgatgag gagatggata agtttctttc tttaattaaa gatatggaat gagggatatt
     1561 atggacagga aagcgtttgt aaaaagagaa acaagagaaa caaaaattga agcttcaatt
     1621 aatttggatg gtggtgcata tgatatcaat accggcatag gtttttttga ccatatgttg
     1681 gagctttttg caaaacattc gggtataggt ttgtctgtgg aagcaaaagg cgatttgtat
     1741 gtggactgcc atcatactgt ggaggatgtt ggtattgttg ttggtacttt gcttaaggaa
     1801 gctgccggca gcaaggatgg aataaaaaga tacggccata gtgtaatacc catggatgag
     1861 gctttaagcg aggttgtaat tgattttggc ggcagatcct tctttatatt gaagggtgaa
     1921 ataccgtgca tccgtttggg agagtttgaa acggaaaccg ttcaggagtt tttcaccagc
     1981 attgcaagca atgccggaat gaacctccat gttattgttc gctatggaac gaatacgcat
     2041 catataattg agagtgtttt caaggccttt gcgcacgcct tgaagcaggc aataagttat
     2101 gatccgaatc aaaagggagt attatcgacc aaaggagttt tgtaatatgg acaaggaatt
     2161 aattaaggat ataaagttca atgaagaggg aatggtgcct tgtattgttc aggactgtag
     2221 aacaggtgaa gttcttatga tggcctggat gaataaggag tctttggaga tttctcttga
     2281 tacaggtttc tgtacttact attccagaag ccgaaaggcc ttgtggaaaa agggcgagac
     2341 cagtggaaat gtgcagaaaa ttgttgacat ggcttatgat tgtgacggag atacattact
     2401 tgttctggtt gagcagacag ggagtgcatg tcataccggt gaaaggtcat gttttcataa
     2461 tccttcacct gtttttgtta aacataatat tttatacgag gattatgatg tcatagcgga
     2521 cagaaaagca aatcccagga tgattcttat accaattatc tgttcaggga aggtattgac
     2581 aagatttgca aaaagatagg ggaagagagt gcggaaatca ttatagcctc caagaatgaa
     2641 attaagaatg atactgttgg agaaattgcg gactttatgt accatttgat ggtgcttatg
     2701 gtggagtgcg gtattacatg ggatgacgtt tatgacaggc tgttagaaag aaaaggtata
     2761 aaatcggaaa agttgagcaa attaatgaaa aaagaagtaa aagagtagtt ttatatcaaa
     2821 ttttggagtt tttgaagaaa aactttacat ttgggtcttg attatttttg aaatattatt
     2881 ataattatta ttagcactca tttgaagtga gtgctaataa tatgaaaaga taataaaagg
     2941 aggaatatgc atgaaaataa aacctttagc tgacagagtt gtaatcaaaa tgttggaaca
     3001 ggaagaaaca acaaaaagcg gcattgtatt gccaggttca gctcaggaga agcctcagct
     3061 tgctgaagtt gtggctgtag gtcctggtgc agttgtagat ggaaagaaag tgcctatgga
     3121 agtcaaggta ggcgacaaag taataatgag caaatacgca ggtactgaag tgaagtttga
     3181 tggtgttgag tacactattc ttaagcagag cgacatactt gctattgtag aataaaatag
     3241 atagatggag gcgatgacga atggcaaaag aaattatgtt tggcgaagag tcaagaaaag
     3301 cattgttaaa tggtgtaaac aagttggctg atacagtaaa gattacatta ggtcccaaag
     3361 gaagaaatgt agttctcgac aagaagtttg gctcaccctt gattaccaat gatggtgtta
     3421 ccattgcaaa agaaattgaa tttgaagata gatatgaaaa tatgggtgcg cagctcgtca
     3481 aggaagttgc aaccaagacc aatgacgtgg ctggtgacgg taccacaacc gctacattgc
     3541 ttgctcaggc aatgattaga gaaggaatga agaacattgc agctggagca aatccaatga
     3601 ttctaaagag aggaattgca aaagctgttg aggttgctgt tgagagcatc aaggaaaaca
     3661 gccagaaggt aagaggccgc gaggatattg caagagttgc ttctatatcc gctaatgacg
     3721 aagttatcgg aaaccttatt gctgatgcca tggagaaggt aaccaacgaa ggtgttatta
     3781 ccgttgagga atccaagacc atgggaacaa gccttgaagt tgttgaaggt atgcagtttg
     3841 acaggggtta tgtatccgca tacatggcta ctgacactga caagatggaa gcggtgcttg
     3901 atgatccata catcctcatc accgacaaga agatctccaa tatacaggaa cttttgccag
     3961 ttcttgagca aatcgttcag cacggcaaga agcttctcat aattgctgaa gatgtggaag
     4021 gtgaagcttt ggctacactg gtactcaaca agttgagagg cacattcact tgcgttgctg
     4081 tcaaggctcc tggttttggc gatagaagaa aagaaatgct cagagacatc gctatcctga
     4141 caggcggcga ggttattagt gaagagcttg gacttgaact caaggataca accatcgacc
     4201 agcttggccg tgcaagacag gtcaaggttg caaaagagaa caccattata gttgatggtg
     4261 ctggtgattc cgacgatatt caaaagcgaa tcgcttcaat caaagctcaa atcgaagaga
     4321 caacttccga gtttgacaag gaaaagctca tggagagact tgcaaagctg gcaggcggcg
     4381 tagctgtaat ccaggttggc gcagcttcag aagttgaaat gaaagaaaag aaactcagaa
     4441 tcgaagacgc attgtctgct acaagagctg ctgttgagga aggtatcgtt cctggtggtg
     4501 gaaccgcata catcaatgca atttcaaagg taacagagct tctagacaag actgaaggcg
     4561 acgaaaaaac tggtattatg attgttctta aagcgcttga agagcctctc aagcagattg
     4621 caatcaatgc cggacttgaa ggcgctatcg ttgtagacag agtcatgaag gaagacaagg
     4681 gcgttggctt tgatataaac agtgaggaat atgtggatat gatttcccac ggtattgtag
     4741 atcctgcgaa ggttacacgt tcagcattgc agaatgctgc atcagtagct gcaatcctgc
     4801 ttacaacaga agctgcggta tctgatattc ctgagcctga gccagcagtt ccagcaggtg
     4861 gaatgggcaa cggcatgtac taaaaggttt taattagaaa cattttgtgt tatactatga
     4921 aggtcacaat aaaattgtgg ccttcatata ttattcattt caggtggtgt gataatggag
     4981 ataattatta aaagggaaac ggattccttt tacacttttt tgagtttcat gataatggtt
     5041 gccgctgtgg ttttgcttgt attgttatac aattttgctg gatacatcgt ttttgttccg
     5101 acacccttta ttctttttgc tgcaggcata gtgtccaggc tggtgtacat aaattacaac
     5161 aaggtggagt ttgagtactc actgcattcg ggaattttca atattgacaa acttgttaac
     5221 caggcaaaaa gacagcctgt tataaaattg ccagcttccg atatccttac atttggcagg
     5281 tataatgata gtgttgatga agaatataaa gatggcacca agcatgttga ttgctcaagc
     5341 ggcagagatg atgtggactt atattacttt acatgccacc agaaccaagg aagattcttg
     5401 ataatatttg aaccaaacga aaaattgctg gcgatgctga aaggttatag cagtgtcgta
     5461 gctaaggctt tactaaaaga caacaaataa ttaatttgac atataaatat gcaaacatta
     5521 taatatcttc cactaacttt atatattatg gattttaagg agaaagatca tggcatatat
     5581 ttacgatgaa ccgtcgcata catttagcga atatttacta ttgccaggtt tggcaacaaa
     5641 acagtgcata ccaagcaacg tgtctttaag gactccggtt gtaaagtata aagaggggga
     5701 ggaatctgaa atttacatga acatacccct cgtttctgcg atcatgcagt cggtatccga
     5761 tgacaagatg gcaattgctt tggccaaatg tggaggtttg gccttcattt atggttctca
     5821 gtcaattgag gagcaagcag agatggttaa aagagtcaag aagtacaagt ctggatttgt
     5881 tgtaagcgat tcgaatctga ctcctgaaaa tacccttgaa gatgttttga atcttaagaa
     5941 ggaaaaaggc cattccacca ttgctattac tcatgacggc tctcccaacg gataccttat
     6001 gggaattgtt acaagcaggg attacagagt cagcagaatg agtcctgaaa caaaaataaa
     6061 ggatttcatg actccctttg agaagcttgt ttttgcaaga gaaggaatta cacttaaaca
     6121 ggcaaatgat ataatctggg accacaaact caacaccctg ccaattatag atgaggaaaa
     6181 tcgtcttaga tatctggttt tcagaaagga ctatgaccaa cacaaggaaa atcccgtaga
     6241 actgctcgac aaggaaaagc gctatatgac cggtgcaggt atcaacacaa gagactacag
     6301 ggaaagggtg cccgagcttg taaatgccgg agttgacgtt ttgtgcatag attcctctgt
     6361 aggttattcc gaatggcaga aggataccct tgagtttatc aagacggaat acaacggcaa
     6421 ggtcagatgc ggtgcaggta atgtagtcga tgcagagagt ttcctatacc ttgcggaagc
     6481 tggtgctgac tttatcaaaa tcggtattgg tggaggttcc atctgcataa ctagagagca
     6541 gaagggaata ggaaggggcc aggcttcggc agttattgag gttgccaagg ccagggacga
     6601 gtacttcaag aagacaggca tctatattcc tctttgttcc gatggcggca ttgtacatga
     6661 ctatcacata actcttgcat tggcaatggg tgcggatttc gtaatgcttg gcagatattt
     6721 tgcaagattt gacgaaa
//
LOCUS       DAONVY010000049         6725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8536_length_6725_cov_4.147526, whole genome shotgun sequence.
ACCESSION   DAONVY010000049 DAONVY010000000
VERSION     DAONVY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_6725_cov_4.147526"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLI11_02220"
     CDS             <1..254
                     /locus_tag="PLI11_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51710.1"
                     /translation="LNVRDDYVSVYGNTAGSVVLYTLQWMHSSLNEGDYIPIVAATSG
                     SAISVTDKQKDMLGIFSIIAWPACIMIAGGVVWFRRRHL"
     gene            251..1645
                     /locus_tag="PLI11_02225"
     CDS             251..1645
                     /locus_tag="PLI11_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPZ51711.1"
                     /translation="MKQYKVTFISLIVLAVVLAGYFLISGLIGGEPSNDDQDQVRRER
                     VLAFNPNRVVKIETHYKEDFVLEKIDDKWVCTSHDDLKVHESSIISILNSYANMQGNV
                     IVGEDETIIVENFGFHDDPPTFTIYLEDGKSHKLLLGNTTLSGSEIFCMFEGGDKVYK
                     ISGTFTERLQVTRATITTNKVFPNINKERINTVELVRHGETKYIIRGDFSGDEKAWKL
                     TYPFTIKGDSTQINSLVDTLMALTVGDLVSTNVEDLSEYGLDVPVAKYTVKDNQTTRT
                     LEIGDRTSDGYNRYCTVNGENHVYLIAMSKLSFVDNEVTMYMNTYPFLENQIVLSSVK
                     LTIEGEVWNLDYEVTDTEENYWFNGVNVNRPNKDLRSQFKKITTAMYSLRLEEIDLEE
                     PKPKELICSIEYTRTDNTKVLVEYYRRSENTCYIYVDGEFMNGMIYTRRITGDTSDSL
                     INVVRNFESLMNEN"
     gene            1713..2876
                     /locus_tag="PLI11_02230"
     CDS             1713..2876
                     /locus_tag="PLI11_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51712.1"
                     /translation="MRKNKVINVMKLGFLYLSTVIGAGFATGREIRVYFTDYGFFGYV
                     GLGISTILLILAGCAILRIVYVNDVKTVSEFNERIGGFYLGKVLDFVVTSFSYCLYVI
                     VLAGLADILKSNYGIDKILTVLTVMVISIMVHIMGFNALAHICSWSAILIAVMVLFVS
                     LFTIKPVYSFEQTVSIPMSLFSSVLYVGYNILVAISVMSRALPLMDGKASYIVGSFIG
                     SVSIMACALVVNISLYMSYGRIKYSDMPLLTLVELYMGKWGYYLFLLMLVLTMSTSAI
                     SGLSSVLHVAGNKLGRVLVVVSIPLSFIGFGTLMDIAYPVYGAFGIMVLFILVLSVGM
                     NYNRVRDDQCDRQKNRYKLRTTKKKKHAAKARSYNISHTGNCNSTCNVCHRCV"
     gene            2935..3963
                     /locus_tag="PLI11_02235"
     CDS             2935..3963
                     /locus_tag="PLI11_02235"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HPZ51713.1"
                     /translation="MIDYSPKGESASASLKDTIIIADESGVTGLDVEGKWKWNYVYEL
                     FEPVLETYNNLVLLTDLGGYSVFAFDENGLLWHMVFDKGIIAAYYDESTKYFLALHND
                     EDYKTCITVYDVKKGNKPLFTRKFGAYYMTKADISSDASQMAVSGFYQEAGISTAVVT
                     FLRMRDGEVYTTETFHDNICPYISYLKENALFIASTDQIVRIVRETTASSKMDTEKVI
                     WERNADSTGLVCVNSFRDDYLIAAFSEINTNLVSDTTTSLIRIYNNKGEVVRKIELDG
                     KARGISTGKDTFAVYTDHTVYLYNLKGFLISKFNLVSDIQGVSYLDTRVLFVSGQNQM
                     AVVDMSGK"
     gene            3983..4684
                     /locus_tag="PLI11_02240"
     CDS             3983..4684
                     /locus_tag="PLI11_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPZ51714.1"
                     /translation="MNIADIVVIVVILLFAFLGYRRGLLMGIYSIGAYFIAIFIGFLL
                     RKPVVAFIYKTPLPDKVYNNVYARLVEHNVSKGQEALANTEDYIESLKLPSLIENFLK
                     KNIQTGEGAFEALAESISDKVTAFIVSVFAFLITFILVLIIMLVLKRIIKFARELPVI
                     KQVDSVGGVLLGVLEGILIVSVVLLFIYMFSSRESLAPLVQKIEESSIAKLFFEKNFL
                     ANIISKLKIFAARLF"
     gene            4820..4969
                     /gene="rpmG"
                     /locus_tag="PLI11_02245"
     CDS             4820..4969
                     /gene="rpmG"
                     /locus_tag="PLI11_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPZ51715.1"
                     /translation="MRVNITLACTECKRRNYTSKKSKKNNPDRLELSKYCKFCQKHTA
                     HRETK"
     gene            4986..5219
                     /gene="secE"
                     /locus_tag="PLI11_02250"
     CDS             4986..5219
                     /gene="secE"
                     /locus_tag="PLI11_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPZ51716.1"
                     /translation="MADNKKARNRSNPIKNVARRVKKFFKDLSTELKKVTWPTKKEVV
                     QNTGSVLLFCLLVGGLIWIVDFLLETLVRLIYG"
     gene            5242..5775
                     /gene="nusG"
                     /locus_tag="PLI11_02255"
     CDS             5242..5775
                     /gene="nusG"
                     /locus_tag="PLI11_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPZ51717.1"
                     /translation="MENNVANEPHWYVIHTYSGYENKVKASLESVIENRGMHDYFYDI
                     IIPMLEEVEIKDGREKSSLKKIFPGYVLIKMILTDDSWSLVRNIRGVTGFVGPGSKPI
                     PLSDTEVRALGVEYTETVVDYDVGDTVKVVSGPLESFTGIVEEINVEKERVKVLVSMF
                     GREISAELEYDQLQKIF"
     gene            5807..6232
                     /gene="rplK"
                     /locus_tag="PLI11_02260"
     CDS             5807..6232
                     /gene="rplK"
                     /locus_tag="PLI11_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPZ51718.1"
                     /translation="MAKKVTALIKLQIPAGKASPTPPVGPALGQHGLNIMGFCKEFNE
                     KTKNEVGLIIPVVITVYSDRTFTFITKTPPASVLLKKACKIESGSGVPNKDKVAKISK
                     ADLMQIAEKKMPDLNATDIEAAARMIAGTARSMGIVVEG"
     gene            6275..>6725
                     /gene="rplA"
                     /locus_tag="PLI11_02265"
     CDS             6275..>6725
                     /gene="rplA"
                     /locus_tag="PLI11_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPZ51719.1"
                     /translation="MFRGKKYKESAKLVDRTKLYDPTEALELVIKTAKAKFDETVECH
                     IKLGVDSRHADQQVRGAVVLPNGTGKEKKILVFAKGDKAKEAEQAGADYVGAEELVAK
                     IQQENWFDFDVVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTMDVK"
BASE COUNT         2041 a         1098 c         1607 g         1979 t
ORIGIN      
        1 gtctgaatgt gagggatgac tatgtatcgg tttatggaaa cactgcaggg tcagtagtat
       61 tgtacacact tcagtggatg cattcctctc ttaacgaggg tgactacatt cctattgttg
      121 cagctaccag cggaagtgcg atatctgtaa cagacaagca aaaagacatg cttggaatat
      181 tctcaattat agcatggcct gcttgcataa tgattgctgg aggagtagtc tggtttagga
      241 ggagacatct atgaaacaat acaaggttac attcatctcc cttattgtac ttgcagtcgt
      301 cctggcaggg tactttctaa tctcgggact tattggcgga gagccctcca atgatgacca
      361 ggatcaggta cgaagggaga gggttttggc gttcaacccc aatagagtgg tcaagataga
      421 aacccattac aaagaagact tcgtgcttga aaaaatagat gacaagtggg tttgcacgtc
      481 acacgatgat ttaaaggtgc acgaatccag cattatttcc atattgaaca gctatgccaa
      541 catgcagggc aatgtgatag tgggagaaga tgagacgatt attgttgaaa actttggttt
      601 tcacgatgac cctccaacct ttacaatcta tttggaggat ggaaaatccc acaagcttct
      661 tcttgggaat accacgcttt ccggcagtga aatattctgt atgtttgaag gcggagacaa
      721 ggtgtataaa ataagtggaa cttttacaga aagattacag gtgacaagag ccacaattac
      781 caccaacaag gtgtttccaa acattaacaa ggagagaatc aatacagtcg aacttgtcag
      841 acatggtgag acgaagtata ttatcagggg agacttttcg ggtgatgaaa aggcctggaa
      901 gctgacctat ccgtttacca ttaaaggaga ttctacacag attaactctt tggtggacac
      961 gcttatggcg cttacggtcg gagatcttgt aagcacgaat gtggaggatt tgtcagagta
     1021 cggtcttgat gttcctgttg ccaagtacac tgtcaaggac aatcagacaa caagaacctt
     1081 ggagattgga gacagaacca gcgacggata caacagatac tgtactgtta atggtgaaaa
     1141 ccatgtctac cttattgcca tgtcgaagct cagcttcgtt gacaatgagg ttaccatgta
     1201 catgaatact tatccttttt tggagaacca gattgttttg tcgagtgtaa agctgaccat
     1261 agagggagag gtttggaacc tcgactatga agtaacggac acggaggaga actactggtt
     1321 caacggtgtt aacgtaaaca ggccaaacaa agatctaaga agccagttca agaagattac
     1381 cactgcaatg tactccttaa ggcttgagga gattgacctt gaggaaccaa agcccaagga
     1441 gctcatctgt tcaattgagt atacaagaac tgacaacaca aaggttctgg tggagtatta
     1501 caggagaagt gaaaacacat gctacattta tgtagacggt gagttcatga atggtatgat
     1561 ttacacaaga aggattacag gggacacgtc tgacagtctc ataaacgtgg taaggaactt
     1621 tgaaagcctg atgaatgaaa attaattgaa atattacaag acctcttgga atatctattg
     1681 atagaatcgg atttttcaag aggtcttttg ttatgcgtaa aaacaaggta ataaatgtta
     1741 tgaagctggg ttttctatac ctttcaaccg tcataggagc cggttttgca acgggaaggg
     1801 aaatcagggt atattttacc gattacggtt tttttggata tgtgggactc ggtataagta
     1861 caatattatt gattctggca ggctgcgcaa ttcttaggat tgtgtatgtt aatgatgtta
     1921 aaacggtttc tgagtttaat gaaagaatag gcggatttta cttggggaag gttcttgatt
     1981 ttgttgttac ttctttttct tactgtcttt atgtaattgt tcttgctggt cttgccgaca
     2041 ttctaaaatc caattatgga atagacaaga tactgactgt tcttaccgtg atggtgattt
     2101 caatcatggt tcatataatg gggtttaacg ctcttgcgca catatgttca tggtcggcta
     2161 tacttattgc ggtgatggtt ctttttgttt cgctttttac tataaagcct gtatattcgt
     2221 ttgagcaaac tgtttcaatt ccaatgtctt tgttttcttc tgttttgtat gtagggtaca
     2281 acatactggt ggctatttca gtcatgtcaa gagcgctgcc gctaatggat ggaaaggcaa
     2341 gctatatagt tggttctttt atcggcagcg tttcaataat ggcttgtgca ctggttgtta
     2401 acataagttt atatatgtct tatggcagga taaagtactc cgacatgcct ttgcttacat
     2461 tggtggagtt gtatatggga aagtggggat attatttgtt tctcctgatg cttgtactca
     2521 ccatgtccac atcagccatt tctggtcttt caagtgttct acatgttgca ggcaacaagt
     2581 tgggaagggt acttgttgtt gtttcaatac ccttgtcgtt tatcggattt ggcacattga
     2641 tggatattgc ctaccctgtt tacggtgctt ttggaataat ggttcttttt atacttgtct
     2701 tgtctgtggg gatgaattat aatagggtga gggatgatca atgtgacagg caaaagaata
     2761 gatataaatt acgaacgaca aaaaagaaga aacacgcagc gaaagctcgc agttataata
     2821 ttagtcatac tggcaattgt aatagtactt gtaatgtatg tcacaggtgt gtatgaaaag
     2881 ctcatgatgt atttcggcat caagggtgat gagtatgttg aacctgttgc gaacatgatt
     2941 gattattctc ccaagggtga aagtgcaagc gcttctttaa aggatacaat cattattgcc
     3001 gatgaaagcg gcgttactgg tttggatgtg gaagggaagt ggaagtggaa ttacgtttat
     3061 gagctttttg agccagttct tgaaacgtat aataaccttg tattgttgac agatttggga
     3121 ggttacagtg tttttgcatt tgatgagaac ggacttctgt ggcacatggt ttttgacaaa
     3181 ggcatcattg cagcatatta tgacgaaagc acaaaatact ttcttgctct tcacaacgac
     3241 gaggattaca agacatgtat aacggtttat gatgtaaaaa aaggaaacaa gccgttattt
     3301 accagaaaat ttggggcata ctatatgaca aaagctgaca tttcaagcga tgcctcccag
     3361 atggcggtaa gcggattcta ccaggaagcg ggtatttcta ctgcggtggt tacatttctt
     3421 agaatgaggg atggggaagt atacactacg gaaacattcc atgacaacat ctgcccttac
     3481 atttcctacc tgaaggaaaa tgccttgttc atagccagta ctgaccagat tgtcaggatt
     3541 gtgagagaga cgacagcgtc atccaaaatg gatacggaaa aagtcatctg ggaaagaaat
     3601 gcggacagta cgggcctggt ttgtgttaat agttttagag atgactatct tatagctgcc
     3661 ttttcagaga ttaatacaaa ccttgtgtcg gatacaacta ccagcttgat aaggatctac
     3721 aacaacaagg gggaggttgt aaggaaaatc gaattggacg gaaaggcaag gggaatttca
     3781 acagggaagg atacgtttgc agtgtatacg gatcataccg tatacttgta caacctaaag
     3841 gggtttttga ttagcaagtt caatcttgtt tctgatatac agggtgtaag ctacttggat
     3901 acacgagttc tttttgtatc cgggcagaat caaatggcag tggtggacat gtccggcaaa
     3961 tgacagacgg gaggaatgtt gaatgaatat tgctgatatt gttgttattg ttgttattct
     4021 tttgtttgct tttctaggct acagaagagg actcttgatg ggaatctatt caataggggc
     4081 gtattttatt gcaatcttta ttggcttttt gttaagaaaa cctgtagttg cgtttattta
     4141 caaaactcct cttcctgaca aggtgtacaa caatgtgtat gcaagactgg tcgagcacaa
     4201 tgtttcaaag ggtcaggaag ctttggctaa tacagaagac tatattgaga gtttaaagct
     4261 tccttcactg attgaaaatt ttcttaagaa gaatatccaa acaggggaag gtgcgtttga
     4321 agctcttgca gaaagcatta gcgacaaggt aactgcgttt attgtcagtg tttttgcttt
     4381 tttgataacg tttattcttg ttttgattat tatgcttgtt ttgaaacgta tcatcaagtt
     4441 tgcaagagaa ctgccagtta ttaaacaggt tgattcggtg ggtggtgttc ttctaggagt
     4501 tttggaagga attttgattg tatctgttgt gcttcttttc atatacatgt tctcttcaag
     4561 ggaaagtctt gcaccactag tgcaaaagat agaggagtct tcaattgcga aattgttctt
     4621 tgagaagaat tttctggcaa acattatttc aaagttaaaa atttttgctg cacgcttgtt
     4681 ttagtggttg atttctagtt ttatatattg tattatgata tggtgtgttt cacaatttgt
     4741 taaaaaaatg tgttgttgac attcataatg gattgtgtta aattatacag tgcgctttat
     4801 tttattgggg gtgtattaag tgagagttaa cattacactt gcatgcacgg aatgcaagcg
     4861 aagaaattac acaagcaaga aaagcaaaaa gaacaatccg gacaggcttg aactcagcaa
     4921 atactgcaag ttctgccaga aacacactgc gcacagagag acaaaataaa aggtaggtag
     4981 acaaaatggc tgacaacaag aaagcaagga acagaagtaa tccaatcaaa aatgtcgctc
     5041 gtagggtgaa gaagtttttt aaggacttgt caactgaact aaaaaaggtg acctggccta
     5101 caaagaagga agttgtccag aacaccggtt cggtacttct tttttgcctg cttgtgggag
     5161 gcttgatttg gattgtggat tttctgcttg agactttggt aagactgatt tacggttgat
     5221 tagaggtgtg gtagagtgta tatagaaaat aatgttgcaa atgagccgca ctggtatgtt
     5281 attcacacat attcaggtta cgagaacaaa gtaaaagcaa gtcttgaaag cgttattgaa
     5341 aacaggggta tgcatgacta tttttatgat atcattattc ccatgctgga agaagttgaa
     5401 atcaaggatg gtcgtgaaaa atcctctctc aagaagattt tccccggcta tgttcttatt
     5461 aaaatgatac ttaccgacga ctcatggtct ctcgttagaa atatccgcgg tgtgaccgga
     5521 tttgttggac ctggttcgaa accaatacca cttagcgata cggaagttag agctcttggt
     5581 gttgagtata cggagactgt tgttgattac gatgtaggcg acactgtcaa ggttgtttca
     5641 ggtcctcttg agagttttac aggaatagtt gaagaaatca acgtggaaaa ggaaagagtc
     5701 aaagtactcg tctccatgtt cggcagggaa atatctgcgg aactggaata tgatcaattg
     5761 caaaaaatat tttaactcat acatgtaatt ttgggaggtg gaatttatgg caaaaaaagt
     5821 aactgcattg attaaactgc aaatacctgc aggaaaagca tcccccactc caccggttgg
     5881 accagcttta ggtcagcatg gcctaaatat tatgggattc tgtaaggaat ttaatgaaaa
     5941 gaccaagaat gaagtaggat tgataatacc agttgttata actgtttatt ctgacaggac
     6001 tttcacattc attacgaaga ccccgccggc atctgtattg cttaagaaag catgcaagat
     6061 tgagagtgga tctggtgtgc caaacaagga taaggtagcc aagatttcaa aagcagatct
     6121 tatgcaaatc gctgaaaaga aaatgccgga ccttaatgca acagacatcg aagcagctgc
     6181 acgcatgatt gctggaacag caagaagcat gggcatcgtt gtcgaggggt aacaagcggc
     6241 aaagtaatga ataaaccata aaggagtgag agaaatgttc agaggaaaaa agtataagga
     6301 aagcgctaaa cttgtagata gaaccaaatt gtatgatcct actgaagctc ttgaattggt
     6361 tataaagacc gctaaggcca agttcgacga gaccgttgaa tgtcatatca aacttggcgt
     6421 tgactcgaga catgccgacc agcaagtcag aggtgcggta gtattaccta acggaacagg
     6481 taaggaaaag aagattcttg ttttcgcaaa aggtgataaa gccaaggaag ctgaacaagc
     6541 aggagctgat tatgtaggtg cagaagaact ggttgccaag attcagcagg aaaactggtt
     6601 cgattttgac gtagtagttg caacaccaga catgatggga gttgtaggac gccttggtag
     6661 ggtactgggt ccaaaaggat taatgccaaa cccaaaatcc ggtactgtaa ccatggatgt
     6721 aaaga
//
LOCUS       DAONVY010000050         6698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8589_length_6698_cov_3.285865, whole genome shotgun sequence.
ACCESSION   DAONVY010000050 DAONVY010000000
VERSION     DAONVY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8589_length_6698_cov_3.285865"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..947)
                     /locus_tag="PLI11_02270"
     CDS             complement(9..947)
                     /locus_tag="PLI11_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPZ51720.1"
                     /translation="MEKANLLPVPREFKYNNKDRTGYSYEAEISCNVEEWESSINAFY
                     QYVERSCGLKLKNGNKGIRILREKELDEDAYRIECNNTVNIYASSKEGLNYALSTVLQ
                     LIQTKDDKILFPDITVYDKPDSSYRALLVDLARQWHPYEYLYKYVDLCYLYKINRLQF
                     HFTDDQSYTLPSDTYPFLPTENRHYTKEQIRDLVEYANDRGVVLVPEVEVPGHCAQFN
                     MKYPEIFGNNGIICAEEKTFQALEKLFGEVCEMFPYSPYIHMGGDEAAITNWRNCPGC
                     RQYMNDRGLKFIDDLYTDFVARVTDIIVKHRRTPVV"
     gene            complement(1025..2245)
                     /locus_tag="PLI11_02275"
     CDS             complement(1025..2245)
                     /locus_tag="PLI11_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPZ51721.1"
                     /translation="MRIKLIFTVLIVCVLLCLSLIIGFAEDKAEIVKLEAENATGQYV
                     TVDDAGASGGRFIGVDHTLDNENNIQYYLVYENAPKCTEIKMIYATPGMGDVVVYVEE
                     NGRNIAVGSIYFPPSGDWYPYGGDELEASAVGFYIPEGSKVTMIATGSVNIDYFEFIY
                     NEEDVPKPEDGKQDEDVKNLNNVFLSDLKWVNSSCHNQESTFSVHTDRNYLFEPLQVA
                     GTVYDKGLGMTSGHYEGAVFVEIDIEGLGFTTFASYVGLDDKTVPNLIESNAQFIVEV
                     DGEEKARSGILKMGDDPELLVVDITGAKRLKLYTSPAGDGIGIDCCVWGNAALGRSTN
                     VDEIFATPTPAPTPEPTPTAVPTDTPEKTGSSKNEDEQDNTKIYIIIVAAALVVAAII
                     VVISIKKKAKDKGI"
     gene            complement(2251..2841)
                     /locus_tag="PLI11_02280"
     CDS             complement(2251..2841)
                     /locus_tag="PLI11_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51722.1"
                     /translation="MRKRVFVFVAFFIVTSFLLSFAEQTTLKMEAEDTEGKYVVVEAQ
                     EASAGKYIGIDHTLDENNENNYYLIFKDMPATTAIKMVYGTGSVVGGEVKIYILEGEQ
                     EKEVGALAFESTGGWDPFGESMLVSVAEDLTIEEGSTIVMKATMSVNIDYFEFVVAES
                     TNPKTSDTLEMIAASVMVVSCLVVFRKKHPLACIAR"
     gene            complement(2943..3788)
                     /locus_tag="PLI11_02285"
     CDS             complement(2943..3788)
                     /locus_tag="PLI11_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51723.1"
                     /translation="MYDVSGDFGQVRIIRNMIPHANKGVLDYYLSVGWHNCNSLYRIN
                     KPQGNSGILMMFTVGGEGVLIFNKNRYVLKHHMACIVPAGQPCEYFTPGNKMWEFYWI
                     HIVGANNLGLINYMIENKGYVFTINNTETITARIEQLLTGYSNDLDSSRAISQLIYDL
                     LENTNLNTSASKEQGTVDRVIKFIRDNYKKDITIKDMARVVHVSPDHLIRVFKKETGF
                     TPYNYLKNYRLLKACDLLSFTNMSVKQISMEIGYKSESSFISQFKSFKGAAPGEYRTK
                     TPFDN"
     gene            complement(3905..4609)
                     /locus_tag="PLI11_02290"
     CDS             complement(3905..4609)
                     /locus_tag="PLI11_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPZ51724.1"
                     /translation="MNGFSTNTGIITTVKGDVTGDGILDTISLVGVFTQDSIYLRNIT
                     LSVQDGMTGAIRNVPLRVDSGFGPTITLFDFMGNGVPDILISIASGGSGGIIFYYVYS
                     FMNFMPRLMFDFEEYDEKYKYTVIYLDYYKVEVTSLYNNERYLIDISNRGIDYLSEIY
                     DEAGRLKEPIEGFVDPLSGLFPIDFESDGKYELFGFQGISGRYHADRLGYINNTLSWR
                     NGEFKLTRQQVAIFGT"
     gene            4973..5125
                     /locus_tag="PLI11_02295"
     CDS             4973..5125
                     /locus_tag="PLI11_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51725.1"
                     /translation="MQLYTIGREFSAGKIVPVKCVCSDSLERINIKDKSFLLVILEEG
                     SLTFEI"
     gene            5255..5893
                     /locus_tag="PLI11_02300"
     CDS             5255..5893
                     /locus_tag="PLI11_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51726.1"
                     /translation="MRLDFIRSQGYFDFAFNHDLFLLEPFMSDTYVIPLHIEYLERIN
                     ECYLRIKENLEMQRDILWSCRARSYFMEVIIVIERLYDVIRNLESSEPEYHEFVKKVL
                     TFIENHYGEDISLNDIIKASESNHTTLGKLFGEELNMTPMKYLWYYRILIAKKHLSFT
                     DIPLKEIAFRCGFKTVSHFSRVFKEHTGKSPLMYRKSAVEERKASMANNVYS"
     gene            complement(5884..>6698)
                     /locus_tag="PLI11_02305"
     CDS             complement(5884..>6698)
                     /locus_tag="PLI11_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51727.1"
                     /translation="LSRIRVIVVKRIACIVMAVVILMSAVGCNSTEKEENPHQPKNYS
                     KEKDLKILAVGNSFFSTSEVIETFKKILIENGKTDIEVDAIFTTSVKSLYSNTYVDGS
                     GTLPEHIKLREGYYDILVLSTIYTDDDVSIISDLLERFKEIDTDIILFPADNEYVNGV
                     RNAVGYYMLNCANWQGYLNRLRFRYNFTSEHLSINDRYMHTTPVGGLAGACLLYARMF
                     DEAPNAESLKTQFITNYYDLFPGETDMEKEESFKIIVNETLNYLKDHDEIYE"
BASE COUNT         1975 a         1469 c         1104 g         2150 t
ORIGIN      
        1 aaacccacct atacaactgg tgtcctccga tgtttgacta tgatatctgt aacgcgagcc
       61 acaaaatcag tatacaggtc atcaatgaat ttcaagcccc tgtcattcat gtactgcctg
      121 caacccggac agtttctcca gtttgttata gcggcctcat ccccacccat gtgtatgtat
      181 ggagagtatg gaaacatctc acatacttca ccaaaaagct tctccagagc ctgaaatgtc
      241 ttttcttcgg cgcatataat gccattgttt ccaaaaatct ccgggtactt catgttgaac
      301 tgtgcacaat gacccggaac ctccacctcc ggcactagta ctacacctcg gtcgtttgca
      361 tactctacca aatcccttat ctgctctttt gtataatgtc tgttttcagt tgggaggaac
      421 gggtatgtgt cgcttggcaa agtatagctt tgatcatcgg taaagtggaa ttgcagtctg
      481 tttattttat acaaataaca aaggtccaca tatttataca aatactcata aggatgccac
      541 tgtcttgcaa ggtctaccag aagtgcccga taggagctgt ctggcttgtc ataaacagtt
      601 atatctggaa acaatatttt gtcatccttt gtctgaatta gctgaaggac agtggataat
      661 gcataattca gtccctcctt cgaggatgca tatatattca ccgtgttgtt gcactcaata
      721 cggtatgcgt cctcatccaa ctctttttct ctaagaatgc gtattccctt attaccgttc
      781 ttcagcttca agccacaact tctttccaca tactgataga aagcatttat cgagctttcc
      841 cattcctcaa cattgcaaga tatttcagcc tcatagctgt atccggtacg atccttatta
      901 ttgtacttaa actcccttgg cacgggcagt aaattcgctt tttccataat atgttcttcc
      961 cttctcccat cacaggatac caatgttatt aacaacatca cacataaaac tactaatatc
     1021 cttctcatat tcccttgtcc tttgctttct ttttaatgga aattacaaca attattgcag
     1081 caaccaccaa agcagcagca actattataa tataaatttt ggtgttgtct tgctcgtctt
     1141 catttttcga agaaccggtc ttttcaggag tgtcagtagg aactgctgta ggagttggtt
     1201 caggggtagg tgcgggcgtt ggagttgcaa atatctcatc cacattggta cttctgccca
     1261 atgccgcatt gccccatacg cagcaatcta taccaatacc atcaccagct ggagatgtat
     1321 acaactttag cctttttgcc ccagttatat ccaccaccaa aagttccgga tcatctccca
     1381 tctttaaaat accgctccga gctttttctt ctccatcaac ctccactatg aattgggcat
     1441 ttgattctat cagatttggc acggttttgt catcaagacc tacataggaa gcaaatgttg
     1501 taaagcccag cccttctatg tctatttcaa caaaaaccgc cccttcataa tgcccgcttg
     1561 tcatgcccaa acctttgtca taaacagttc cagctacctg taatggctca aaaagataat
     1621 ttctgtccgt atgcacgctg aaagtgctct cctgattatg gcatgaagag tttacccatt
     1681 tcagatcgct aagaaataca ttattcaagt tcttaacgtc ctcatcctgc ttgccatcct
     1741 caggttttgg cacatcctct tcattgtata tgaactcgaa atagtcaata tttacacttc
     1801 cagtagcaat catggtaact ttggaaccct ctggtatgta aaagcctaca gcagatgctt
     1861 caagttcatc tccaccatat ggataccaat caccggaagg cggaaaatat attgaaccaa
     1921 ctgctatgtt tcgaccgttc tcttccacat aaactacaac atcccccata ccaggtgtcg
     1981 cataaatcat ttttatctcg gtacattttg gtgcgttttc ataaaccaga tagtattgga
     2041 tattgttttc attatctaat gtatggtcaa ctccaataaa tcttccacca cttgcacctg
     2101 catcatctac tgtgacatat tggccggttg cattttctgc ttcaagtttg acaatttctg
     2161 ctttatcttc cgcaaaacca atgattagcg acaaacataa taacacgcat acgattagta
     2221 cagtaaaaat cagctttatt cgcattcaat tcacctcgct atacaagcca aaggatgctt
     2281 ttttctaaaa acaaccaggc atgaaacaac catgacagaa gcagcaatca tttctaatgt
     2341 gtcactggtt ttgggattgg tactttcagc aacaacaaat tcaaaatagt caatgtttac
     2401 actcatggtg gctttcataa caattgttga cccttcctca atagttaagt cttctgccac
     2461 tgaaacaagc atgctttcgc caaaaggatc ccatccacct gtggattcaa acgcaagagc
     2521 tccaacctct ttctcctgtt cgccttccaa aatgtaaatt ttcacttcac caccaacaac
     2581 tgaacctgtc ccataaacca tttttatagc tgttgtggca ggcatgtctt tgaagattaa
     2641 ataatagttg ttttcattgt tttcgtccaa ggtatggtcg attccaatgt atttgcccgc
     2701 actagcttcc tgtgcctcaa caacaacgta tttgccttct gtatcttccg cttccatttt
     2761 taaagtagtc tgttctgcaa atgaaagcag aaaactagtt actatgaaga aagcaacaaa
     2821 aacgaatact ctttttctca tatgaattcc tcctgctttt tttagaatca tattgaatcc
     2881 aagtattcaa tatcaatagt tttgatttaa caaatcgatt tacagaaact taatgtcgat
     2941 tttcagttat caaaaggtgt ttttgttcgg tattcaccag gcgcagcgcc tttaaaagat
     3001 ttgaactgtg atataaagct gctttcagac ttgtagccta tctccatgct tatctgttta
     3061 actgacatat tggtgaaaga tagtaagtcg catgctttaa gaagcctgta gttctttaaa
     3121 tagttgtatg gtgtaaaacc tgtctccttt ttgaaaaccc ttattaaatg atctggagaa
     3181 acatgcacca ccctggccat gtcttttatt gttatgtctt ttttgtagtt gtctctaatg
     3241 aattttatta cccggtccac cgttccctgc tctttgcttg ctgatgtatt aagattggta
     3301 ttttccaaaa gatcgtatat taactgtgat attgcccttg atgaatccag gtcgtttgaa
     3361 taacccgtca gcaattgctc tattctagca gttattgttt cagtattatt tatggtgaac
     3421 acataacctt tattttcaat catgtaattg attaacccaa gattgtttgc accaactata
     3481 tgtatccagt aaaactccca cattttatta cctggggtaa aatattcgca tggctggccg
     3541 gcaggaacaa tacacgccat gtggtgtttt aaaacatacc tgtttttatt aaatatcaag
     3601 acaccttccc cacccacggt aaacatcatc agtatgcccg aatttccttg tggcttgttg
     3661 atgcgataaa gactgttgca gttgtgccat cccactgaaa gatagtaatc aagaactcct
     3721 ttgtttgcat ggggaatcat gtttctaatt attctgactt gtccaaaatc tccagataca
     3781 tcgtacataa tacacaccct tgttgcttgt tttactcgga ccattttgtt tcacgtacat
     3841 tatatcacac acttgttgac aagtcaggga atcgaacagg tggaattgct taaacgatag
     3901 taatttatgt tccgaatata gcaacctgct gtctggttag tttgaattcg ccgttcctcc
     3961 atgaaagagt attgtttata tatccaagcc tgtcggcatg ataccttcct gatatccctt
     4021 gaaaaccaaa cagctcatac tttccatccg attcaaaatc aataggaaac aaaccgctca
     4081 atggatctac aaagccttct atgggttctt ttaatctacc cgcctcatca tagatctcgc
     4141 tcaaatagtc aattcctctg tttgatatgt caattaagta cctctcgtta ttatacagac
     4201 tggttacctc aactttataa taatccaggt atatgacagt atacttgtat ttctcgtcat
     4261 attcctcaaa gtcaaacatg agtctgggca tgaagttcat gaaggaatat acgtaataga
     4321 atatgatgcc tccggaaccg cctgatgcga tacttataag gatatcaggc actccatttc
     4381 ccataaaatc aaataatgtt atagtcggac caaatcctga atcaacccta agaggtacat
     4441 ttctaatggc tcctgtcatt ccatcctgca cagacaaggt tatgttcctt agatatatgc
     4501 tgtcctgcgt aaatactcca accaaagata tagtatccaa aataccgtct cccgtaacat
     4561 ctccttttac ggttgtaatt atcccagtat ttgttgaaaa gccgttcact atgcttcctc
     4621 caaaagacaa ctatcatatc tattatatgt ttccgcctct ttttttgttc atatgaaaaa
     4681 aggtaactgc tttttacgct gcaccactct cataagggga agagtgttaa atttatgatg
     4741 cagcataacc gacagttacc attttggcaa aattcaagtg cataaatagc ttatgataaa
     4801 ccatacccta aatcaacata taaaattgtc taaaattaaa tattagttgt ctgttatgaa
     4861 aaaattccat acattgaaca aaattgtgcg ttgcaaagta tctgcaagtc atgctatact
     4921 cataaataga acaattatcc aatgtatttc aatatttttc gaggtgactt tgttgcagct
     4981 ttatacaatt ggcagagaat tcagtgcagg taaaatagtg cctgttaaat gtgtgtgcag
     5041 cgacagtttg gaaaggataa atataaaaga caaaagcttt ttgctggtca tactggagga
     5101 aggttccctg acttttgaaa tttaggacaa aatatatgat gcaaagggac cttgctttct
     5161 atgctttgat gaaactgcaa atccttatgt attaaaaagg aaaaacataa aagcaagaag
     5221 tatttacttc catcctcagt tcattaacat aaacatgagg ctggatttca ttagaagcca
     5281 gggatacttt gattttgctt tcaaccatga cttgtttctc ctggaaccct tcatgagcga
     5341 tacatatgta attcccttgc atattgaata tcttgaaaga atcaatgaat gctacctaag
     5401 aatcaaagaa aatttagaga tgcaaagaga cattctctgg tcctgccgtg ccagatccta
     5461 cttcatggag gtaatcattg taattgaaag gttgtatgat gtaattagaa atttggaatc
     5521 cagcgagcct gagtaccatg aattcgtaaa aaaagtattg accttcatag aaaaccacta
     5581 tggtgaagac atctccttaa atgacatcat aaaagccagc gaatcgaacc atacaactct
     5641 gggaaagctc tttggcgagg aactcaatat gacgcctatg aaatacctat ggtactacag
     5701 aatcctcata gcaaagaagc atctgtcttt taccgatatt cctttaaagg aaatagcctt
     5761 caggtgtggt ttcaaaaccg tttcccactt ctccagggtc tttaaggaac acacaggaaa
     5821 atcgcctctt atgtatcgaa aatctgctgt tgaagaaaga aaagcatcaa tggcaaacaa
     5881 tgtctattca tagatttcgt catgatcttt caagtagttt agcgtttcat ttacaattat
     5941 tttaaaagac tcttcttttt ccatgtccgt ttcacctgga aacaaatcat aatagttggt
     6001 aataaactga gtttttaaac tttcagcatt tggtgcttca tcaaacattc ttgcataaag
     6061 aaggcatgca ccagccagtc ctcccacagg tgttgtatgc atatatctgt catttatgct
     6121 caaatgctca cttgtgaagt tgtatcggaa ccttaacctg tttaaataac cctgccaatt
     6181 ggcacagttg agcatgtaat agcccactgc gtttcttaca ccatttacat attcattgtc
     6241 agcaggaaaa agaattatat cagtatcgat ctctttaaac ctctcaagaa gatcggatat
     6301 tatagaaaca tcatcatccg tatatattgt tgatagaaca agaatatcgt aatacccttc
     6361 cctcaatttt atatgctcgg gcagagtgcc agaaccatca acatatgtat tggaatagag
     6421 gctctttaca ctggtcgtaa atatggcatc gacctctata tctgtttttc cattctctat
     6481 tagaattttc ttgaaagttt cgataacctc gctggttgag aaaaaactgt ttccaacagc
     6541 aagtattttt aaatccttct ctttggagta atttttgggc tggtggggat tttcctcttt
     6601 ttctgtactg ttgcacccaa cagcgctcat aagaataacc acggccataa caatgcatgc
     6661 aattcttttc acaactatca ccctgattct tgataaat
//
LOCUS       DAONVY010000051         6691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8603_length_6691_cov_4.284207, whole genome shotgun sequence.
ACCESSION   DAONVY010000051 DAONVY010000000
VERSION     DAONVY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8603_length_6691_cov_4.284207"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..699
                     /locus_tag="PLI11_02310"
     CDS             19..699
                     /locus_tag="PLI11_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPZ51728.1"
                     /translation="MNIERNIDELRERVAKSAAKSGRKPEDIQVVAVCKTVGVDEVIR
                     AYNAGMIDFGENRAQDFLKKYENLADYNLNWHFIGHLQRNKVKYIVDKVKLIHSVDSI
                     RLAKEIDIQARKIGKPVDCLLQLNVSGEEQKYGMDPEMIYEFLEEVSKMEYVRIKGLM
                     TMAPCECPEKELRKIFSRTHEIYVDISKNSMHNINMDYLSMGMSNDFEIAIEEGANMI
                     RVGSSIFN"
     gene            750..1346
                     /locus_tag="PLI11_02315"
     CDS             750..1346
                     /locus_tag="PLI11_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPZ51729.1"
                     /translation="MAKLTETVKNFLGFNTLVEDYDEYEEETYAEDYYEDESMEETRM
                     ASGDDSARSTRISSMVYNNPTPSRKTSTQASSFFEGQSKSSERVKDKQESSRILGIKQ
                     SDNNLSVVLSRPSEYKDCTEICAKLRERTTVVINFEHLKRKEDKMRILDFIYGCCYAI
                     DGNAQRISEHVYIVAPYNVDIYDDVGEEEEYEEEISYF"
     gene            1369..1752
                     /locus_tag="PLI11_02320"
     CDS             1369..1752
                     /locus_tag="PLI11_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51730.1"
                     /translation="MGLDNNANTSKCTYSKIGCFLFVLAINVFFQAIFRSLNLVLFES
                     SAVQNIMQSHIFIPLSCFVSGFFSYLLTGKMAINSIASLITGFFAFLMFVEPRPAAIF
                     WALLYYVNAFLGYGIAFFAKSYRVK"
     gene            1773..2432
                     /locus_tag="PLI11_02325"
     CDS             1773..2432
                     /locus_tag="PLI11_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51731.1"
                     /translation="MIKEFPINTFKNLDMVLVLPRFNGKWALCSDNGTWKCPESFIKD
                     EEDAYEAACRLVSDECGVDDFILIPVWDFELYEENIKGRVYYAKLNTCADSSYALFRN
                     LPENLSVNRELAIEKFAKAEVITVTQILIDYLDTLDEVRTYDIRNRREEGSLKDISFF
                     EIDLYVNDAKNFVFILPQMLQERFDSELCAYRIFNDRYVLNISFSKFNPLFVVRFSIM
                     R"
     gene            2529..2604
                     /locus_tag="PLI11_02330"
     tRNA            2529..2604
                     /locus_tag="PLI11_02330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2562..2564,aa:Met,seq:cat)
     gene            2797..3279
                     /locus_tag="PLI11_02335"
     CDS             2797..3279
                     /locus_tag="PLI11_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPZ51732.1"
                     /translation="MEIRKAVIEDLDDILEIYERARQYMAEKGNPHQWKNGYPPKELV
                     VEDIESGNLYVCDIDGEIESVFYFSIGIEPTYEIIEGAWLDDKEYGFLHRIASRGKHK
                     GMLEYIVGYCFDIHSNLKIDTHEDNKIMQHLLEKYGFLKCGTIWLEDGSPRIAYQKNK
                     "
     gene            3291..4007
                     /locus_tag="PLI11_02340"
     CDS             3291..4007
                     /locus_tag="PLI11_02340"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ51733.1"
                     /translation="MNFRIIENDEEFTYAEFLTIEETCFPKDPYRFEQFKDIQNHFYL
                     VARNKHKAVGYCYTKMHGDSLYIHRIAVLDEYRRQGVGKAMMERTIEHAENCMYSSCM
                     LTVDAVNIPAYELYIKMGFKELQTKFRYSFSANVFERAKDVYCVKKGDLQMFDVFFYI
                     EDERIGSCRYNVSVGGCKDFRIKDPAKYVFEALSALAASIIHESGKIYVMTDDADVAN
                     VLNRLRFENKKVMKEMVLDL"
     gene            4061..4210
                     /locus_tag="PLI11_02345"
     CDS             4061..4210
                     /locus_tag="PLI11_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51734.1"
                     /translation="MDKEQKFNNPIVIPLNGEEKPFEFVMEFEKLAESAAGKKTRIIK
                     DKRSE"
     gene            4316..6133
                     /locus_tag="PLI11_02350"
     CDS             4316..6133
                     /locus_tag="PLI11_02350"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPZ51735.1"
                     /translation="MVKNAKMFLILAVILLIACFVFVSVMSLEMDESVLNPTFTPVPT
                     SSNIKPTPKPIVIEEGVTITASKAPGFYNSGFLLELFCSNEEYRILYTITSHEAFECD
                     ENTKVAKTGEDYVYSGGVMPDIDNLDQGSYFYEESIRIPDKSYSAKDPVRITIIRAIA
                     VNEKNECVTDVYTFTYIIGERTLYGKYFVSICIDEESLYGYEEGIFIKGKTFDDERKR
                     NPERSVNSHFPRNYLQKGIEWEREAHIQIFDIMGNLVVDQKVGLRINGGASRHFSVKS
                     LKIYARKKYGKGKIEFEIFEGLEDNYGRPIKEFDKLIFRNSGNDFTGIMFKDVFLHRL
                     AREMGLDWQEGTPCTVYINGKYYSLMNIRESMDGDYIESRYCIPSEFVDMVGITYESK
                     GFIYTSSYGSETAVSKFEEDMSMLVNTDWSVHDISEMEEVIDVNNFIEYMAYQIYIRN
                     TDWPHNNVLAWRYRGIPSKSFEGRNGKWRFLIKDMDYRNVFVDYFDNTLRDVLGNRMT
                     KGPCLGEVLNSCLDNQEFRERFLSYLLGFCNRITPELVDALLFEMRNDWRTDIGIFWK
                     YHGGDESEWLWRIEQLKTFARLRGDYIRKFVDEFIASLE"
     gene            6235..>6691
                     /locus_tag="PLI11_02355"
     CDS             6235..>6691
                     /locus_tag="PLI11_02355"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51736.1"
                     /translation="MLDRKVKEEILDKIGGETGSILQKAYVNIAHVFDNAEEIRIRAG
                     LPLIVVSRGVHYMITQRGQTGDVNSAYKPNYHEISLIFTQLCRYSVYAYAEDIKNGFI
                     TLQGGHRVGISGRILPDGNMRDISSLNIRISRQIKDCARIFLPYIIRNPR"
BASE COUNT         2180 a         1008 c         1541 g         1962 t
ORIGIN      
        1 agaggttgtc aagtaaagat gaatatagag aggaatatag atgaattaag ggaaagggtt
       61 gcaaaaagtg ctgctaaatc aggtaggaaa cctgaagata ttcaagttgt tgctgtatgc
      121 aagactgttg gcgtggatga ggttataagg gcatataatg cagggatgat tgattttgga
      181 gaaaacaggg cacaagactt tttgaagaag tatgagaacc ttgcagatta caacctaaac
      241 tggcatttta tagggcacct gcaaagaaac aaggtcaaat atatagttga caaggtcaaa
      301 ctgatacatt ctgttgacag tatcaggctt gcaaaagaaa ttgatatcca ggcaaggaaa
      361 attggcaaac ctgttgattg cctgctccag ttgaatgtat ctggtgagga gcagaaatat
      421 ggcatggatc cagaaatgat atatgagttc ctggaagagg tttcaaagat ggagtatgta
      481 aggataaaag gacttatgac catggctcca tgtgaatgtc ctgaaaagga acttagaaaa
      541 atattttcaa ggactcatga aatatatgtt gacatttcca aaaacagtat gcataatatc
      601 aatatggact acctttctat gggaatgagt aatgattttg aaattgccat agaagaagga
      661 gccaatatga taagagttgg ttcaagcatt tttaattgat ttttacagaa gatttttact
      721 atcaacaaaa gacaagggag gcatgcatta tggcaaagct gactgaaaca gtcaaaaatt
      781 tcttgggttt caacacactg gttgaagatt acgacgagta tgaagaggaa acctatgctg
      841 aggattacta cgaagatgag tccatggaag aaacaagaat ggcttccggt gatgacagtg
      901 caagaagcac caggatttcc agtatggttt acaacaaccc aacaccatca agaaaaacgt
      961 caacacaggc gtccagtttt tttgagggtc aaagcaaaag cagtgaaaga gtaaaagaca
     1021 agcaggaatc cagtaggata ctaggtatta aacagtcgga caataatctg agtgttgttt
     1081 tatcaagacc ttctgagtat aaggactgca ctgagatttg cgctaagttg agagagcgaa
     1141 ccactgttgt aattaacttt gagcatctga aaagaaaaga ggacaaaatg agaattctcg
     1201 actttattta cggatgctgc tacgctatag atggcaacgc tcagagaatt tctgaacatg
     1261 tgtacatagt agctccatac aacgtggaca tctacgacga cgtcggtgaa gaggaagagt
     1321 acgaagaaga aatttcatat ttctaatcag acgtgactgg agataaatat gggtttagac
     1381 aataatgcaa acacgagcaa gtgcacatac tcgaaaattg gttgctttct gtttgtacta
     1441 gctattaatg tgttttttca agccatattc cgttctctta atcttgtatt gtttgagtct
     1501 tctgctgtac agaatataat gcaaagtcac atatttattc ctctttcgtg tttcgtttca
     1561 ggattctttt cttacctgct tacagggaag atggcaataa actcaattgc gagtttaatc
     1621 acaggattct ttgcattcct aatgtttgtt gagcccagac cggctgcgat tttttgggct
     1681 ctactttatt atgtaaatgc atttttagga tatggcatag ctttctttgc caaatcctac
     1741 agggtaaaat aacttactgg gtttgataga gcatgataaa agaatttcca ataaatacat
     1801 ttaagaattt ggatatggta ctggttctgc caaggttcaa tggcaagtgg gcactctgtt
     1861 cggataacgg aacttggaag tgccctgaat cctttatcaa agatgaagag gatgcttatg
     1921 aagcagcctg cagacttgtt tcggatgaat gtggtgttga tgattttata ctcatacctg
     1981 tatgggattt tgagttgtat gaggagaata taaagggaag ggtatattat gcaaagctga
     2041 acacctgcgc tgattcatcc tatgcactgt ttagaaattt gcctgaaaat ctttctgtca
     2101 accgcgagct agcaattgag aagtttgcaa aagcggaagt gattacagtt acccaaatac
     2161 tgatagatta cttagacaca cttgatgaag taaggacata tgatataagg aacaggagag
     2221 aagagggcag cttgaaagac atatccttct ttgagattga tctttatgtg aatgatgcaa
     2281 agaactttgt cttcatcttg cctcagatgc tgcaggaaag atttgattca gagctttgtg
     2341 cctacagaat tttcaatgac aggtatgttt tgaacatatc cttcagcaag ttcaacccgc
     2401 tgtttgtggt gaggttttcc attatgagat aagcgtgttt gtgttaacat tttgtttact
     2461 gattcgttgt tgtttttttc gtggattgtg ctataattat aactgttgaa agacaaaaga
     2521 ataattgacg cggagtggag cagctggtag ctcgtcgggc tcataacccg aaggtcgtag
     2581 gttcaaatcc ttcctccgca accaaatcaa gtggtcatga cgattagtta tgaccactat
     2641 tcttttaact gaaaaccagt aagaaatctc ccatccagta gttcaatatc cgggagtccc
     2701 ccgttttccc attttatact tcattttgtc acatagtaaa aatatcttgt acaaactact
     2761 tctaagtgat agtatagtgt gaaaaaagga gtcagtatgg aaatcaggaa ggctgtaatt
     2821 gaagatttgg atgatatact tgagatatat gaaagggcaa gacaatacat ggctgagaaa
     2881 ggtaaccctc atcagtggaa gaatgggtat ccaccaaaag agcttgtggt ggaggatatt
     2941 gaatcaggta atttgtatgt gtgtgatatt gatggggaaa tagagtctgt tttctatttt
     3001 tccatcggga tagagccaac ttatgaaatc attgaaggtg cctggcttga tgataaagaa
     3061 tatggttttc ttcatagaat tgcaagcagg gggaaacata agggcatgct tgagtatatt
     3121 gttggctact gctttgacat acattcaaat ttaaaaatag acacgcacga ggacaataaa
     3181 atcatgcagc atttgcttga aaagtatggc tttttgaagt gtggaacaat ttggcttgaa
     3241 gatgggtctc ccaggattgc gtatcagaaa aataaataaa aatgtgtttt atgaatttca
     3301 gaattattga aaatgatgag gagtttacct atgcagagtt tttaaccata gaggagactt
     3361 gttttccaaa ggatccttac cggtttgaac agttcaagga catacaaaac cacttctatc
     3421 ttgtggcaag gaacaagcac aaggcagttg gatattgtta tacgaaaatg catggagatt
     3481 ctttatacat ccacagaatt gctgttcttg atgaatatcg caggcaaggt gtagggaaag
     3541 ccatgatgga gagaacaatt gaacatgctg aaaattgcat gtattcatcc tgcatgctga
     3601 cagttgatgc tgtcaacata cctgcttatg agctttatat aaaaatgggt tttaaggaac
     3661 tgcagacaaa attcaggtat tcattttctg ctaatgtgtt tgaaagagca aaggatgtgt
     3721 attgcgttaa aaaaggtgat ttgcaaatgt ttgatgtttt cttttatata gaagatgaaa
     3781 ggattggttc gtgcaggtac aacgtgagtg ttggcggctg caaggatttt agaatcaaag
     3841 atcctgccaa atatgtattt gaagcactct ctgctttggc tgcaagcatt atacatgagt
     3901 cagggaagat atatgtaatg acagatgatg ctgatgttgc aaacgtgttg aacaggctgc
     3961 gttttgagaa taagaaggtt atgaaggaaa tggtattaga tttgtagtat actttatgca
     4021 ttttgactta caatactacc attatgattt ggaggtcaaa atggataagg aacaaaaatt
     4081 caacaacccc atagtgatac cgcttaacgg cgaagaaaag ccatttgaat ttgtgatgga
     4141 gtttgagaaa ctagcggaat cggcagcagg aaagaaaaca agaattataa aggataaaag
     4201 gagcgaataa aaaaatgctc ctttttttat gttatgttta taaagtgttc attctttttt
     4261 atgattcgtg tagtaccatt taaataaatc tggaaaagtt gtaggaatga gtattatggt
     4321 taaaaacgca aagatgtttt tgatattggc agttatactt cttattgcct gctttgtgtt
     4381 tgttagtgtc atgtcattgg agatggatga aagtgtcctt aatccaactt ttactcctgt
     4441 tccgacttcc tctaatataa aaccaactcc caaacctatt gtcattgagg aaggtgtgac
     4501 aataacagca tccaaagccc ccggctttta taactctggt tttctgttgg agcttttctg
     4561 cagcaatgag gagtacagaa ttctgtatac gataacaagt catgaagctt ttgagtgtga
     4621 tgagaacacc aaggtagcaa aaactggaga agattacgta tatagcggtg gtgttatgcc
     4681 tgatattgac aatcttgacc agggtagtta tttctatgaa gaatctatac gtatacctga
     4741 caagtcgtac tctgcaaagg atcctgtgag aattacaatt atcagggcta tcgccgttaa
     4801 cgagaaaaat gaatgtgtga ctgatgtata tacctttacg tacataatag gagaaagaac
     4861 tttatacggt aaatattttg ttagtatctg catagacgaa gagagcttgt atggatatga
     4921 ggaaggtatt tttattaaag ggaaaacttt tgatgatgaa cgaaaaagga accctgaaag
     4981 aagtgttaac agccatttcc caagaaacta tctacaaaaa ggcattgaat gggaaagaga
     5041 agcccatata cagatatttg acattatggg aaatctggtt gttgaccaga aggtgggttt
     5101 aagaataaat ggaggagcat ccagacattt ttctgtaaaa tctttgaaga tatatgccag
     5161 aaagaaatat ggaaagggta aaatagagtt tgaaattttt gaaggtttgg aagacaatta
     5221 tggaagacca ataaaagagt ttgacaaact tatttttaga aactcgggca atgattttac
     5281 cggcattatg ttcaaggatg tttttctgca cagactggcc agggaaatgg gacttgactg
     5341 gcaggaagga actccctgta cggtatacat aaatggtaaa tattattctt taatgaacat
     5401 aagggagtcc atggatggag attatattga gtcaaggtat tgtattccta gtgagtttgt
     5461 tgacatggtg ggaattacct atgagagcaa gggcttcatt tatacctctt cttacggttc
     5521 ggaaactgca gtaagcaaat ttgaagaaga catgtcaatg ctagtaaata cagactggtc
     5581 ggtgcatgat atttctgaaa tggaagaagt cattgatgtg aacaacttta ttgagtacat
     5641 ggcttatcag atatatataa ggaacacgga ttggccgcac aacaatgtac ttgcttggag
     5701 gtatagggga atacccagca agagttttga aggcagaaat ggaaaatggc gctttttaat
     5761 aaaggatatg gactacagaa atgtttttgt agattatttc gacaacaccc tgagagatgt
     5821 tcttggcaac aggatgacca aaggtccgtg tttgggtgag gttttaaatt catgtcttga
     5881 taaccaggag ttcagagaaa gatttctaag ctatttattg ggtttttgta acagaataac
     5941 tcctgaactg gttgatgctt tgctttttga gatgaggaat gattggagaa ctgacatcgg
     6001 gatattctgg aaataccatg gaggtgacga aagcgaatgg ctgtggagga ttgaacagct
     6061 aaagaccttc gcaagattga gaggagacta cataagaaag tttgttgatg aattcatcgc
     6121 gagccttgaa taggggctct tttttttgcc ctttttggta ataacggtac agcctcatta
     6181 atataatagt acaaaccatg ggttgggaca tgctgcaaca aggaggtgtg aagtgtgttg
     6241 gatagaaagg tgaaggaaga gatactagac aaaatcggag gagagacagg ctcgatactt
     6301 caaaaggctt atgtgaatat agctcatgta tttgacaatg ccgaggagat tagaataagg
     6361 gcaggtcttc ctttgatagt tgtatcaaga ggagtccatt acatgataac gcaaagggga
     6421 cagaccggtg acgtaaactc tgcttacaaa ccgaactatc atgagatttc cctcatattt
     6481 acccagcttt gcaggtattc ggtttatgcc tatgcagaag atattaaaaa tggattcata
     6541 acgctgcaag gcggacacag agtaggtata tcgggacgaa ttctccctga tggcaacatg
     6601 cgcgacatat ccagcctcaa tattcgcatc tcaagacaga taaaggactg tgcaagaata
     6661 ttcctccctt atatcatacg aaatccaaga g
//
LOCUS       DAONVY010000052         6639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8686_length_6639_cov_4.032351, whole genome shotgun sequence.
ACCESSION   DAONVY010000052 DAONVY010000000
VERSION     DAONVY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8686_length_6639_cov_4.032351"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLI11_02360"
     CDS             complement(<1..779)
                     /locus_tag="PLI11_02360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPZ51737.1"
                     /translation="MYDFEKYIKPVWEGNMVIDESFMPIEKEDGSIDPIPLLYKAEKI
                     ISVKNSKHTITYQEGADYILKDGKLEILKDGNIEKVKYKDYYLDENVPGGCFPRTGGG
                     FIYFGEADAMHKLQYVVTYIHGETWQGPLPPYQGVNLPITISKLKNKEPLRIVVYGDS
                     ITTGANSSKVINQMPHIDDWCTMTVKALKKAYASDHIESINTAVGGTVSSWGAEHAYD
                     RAAKFKPDLVFIAFGMNDGSGRVSKDDYLRHIKTIMDTVWEV"
     gene            complement(795..1691)
                     /locus_tag="PLI11_02365"
     CDS             complement(795..1691)
                     /locus_tag="PLI11_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51738.1"
                     /translation="MNQKINTLLMSFTLFFLILLLIFSLVGLRRVRNTIMNLENRIMQ
                     LNNKVNSLQSEISSINSNIQHELKKQASILSSYDIKLGEINASDLTVEAIVEVVPKEI
                     ADDSEMEVTIGDITVIAEKISNKFLAKTYVDALQTYEVVVSVKTNGVSKIETLDRRVD
                     TRRIFSEKAKGLVEGRVLFKNNKLTLEENIINIVYKAFENTEIEMARIFATVDNMEVW
                     EKDITADLKSAEGVLTYTLSKTFDVSAGSELFLYLDVKETNGITFRITMRGLKPYNEN
                     DIVFMDTFAIAIIDKNGKMTLI"
     gene            complement(1705..2139)
                     /locus_tag="PLI11_02370"
     CDS             complement(1705..2139)
                     /locus_tag="PLI11_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51739.1"
                     /translation="MLLLIFFFIIPITIGLCFLQAYLSRKKSKWPGLILPAVFILLSL
                     IWVSYFLSMNNRFGPNIKTPDTRYTYDSEGNIKEEFKKVDGHVSFDRHSRPRVIISGI
                     GLITILITTNIPTVIYLLIYGISREKMRAKLEMQKMAIQDLN"
     gene            complement(2139..2444)
                     /locus_tag="PLI11_02375"
     CDS             complement(2139..2444)
                     /locus_tag="PLI11_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HPZ51740.1"
                     /translation="MAIDIVPEWMRELEEEDVNFIRNFILASGSLKEIANQYNVSYPT
                     VRLRIDKIIQKIRISESRENDPYISLIKRLAVNDKIDFETAKLLINEYKKRMERGNE"
     gene            2694..3053
                     /locus_tag="PLI11_02380"
     CDS             2694..3053
                     /locus_tag="PLI11_02380"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPZ51741.1"
                     /translation="MIFIAGINDGEKKLNYRQEILCKECNRQGFLEIIMYSRYLSLFF
                     IPVFRWKKEYYATTTCCNTMYKIDDEIIKRLKRREISHIPDEELKRNYFYEKTCANCR
                     YVSPPDFAYCPKCGSRL"
     gene            3057..3665
                     /locus_tag="PLI11_02385"
     CDS             3057..3665
                     /locus_tag="PLI11_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPZ51742.1"
                     /translation="MEYFEYGKDEVEYLKSRDKVLGKAMDSVGHIDREIIPDLFEGIV
                     YNIVGQQISMKAARTVWGRFTNKFGKVTPETLYNQDVEDIQKLGVSFRKAGYIKEVSR
                     QVCEKELVIDELYKLPDDEVVKRLVKLPGIGVWTCEMLLIFSLNRMNVLSWQDLAIKR
                     GIINLYGLDSLDKETFNELKERYSPYASVASLYLWEVAKNNG"
     gene            3726..4436
                     /locus_tag="PLI11_02390"
     CDS             3726..4436
                     /locus_tag="PLI11_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPZ51743.1"
                     /translation="MEKDRRFAVLIDADNVSDRYIKLILDEVSNEGILTYKRIYADWT
                     KPSLSSWKNLLLANSITPIQQYSYTAGKIATDSAMIIDAMDILYSENVEGFCIVSSNS
                     DFTRLAVRLREAGKYVIGMGDRKTPQAFVVACNRFVFLEALEKDKAGEIPLESVTSLD
                     VIKDAIKQIVDDTSDEEGWCPLSDVGNTLLKRYPDFDVRNYGFTKLTPLIKHLNCFEI
                     KSVAVNENIKLIYIRNTE"
     gene            complement(4445..5122)
                     /locus_tag="PLI11_02395"
     CDS             complement(4445..5122)
                     /locus_tag="PLI11_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPZ51744.1"
                     /translation="MIHDLHSHTYYSACGRDKPEVIIQAAIDGGIEVFGISDHNYGIG
                     DRKREYYELLTSLKEVYKDRIKLLCGIEVCTLRRDLYLKDDEDISCFDYCLVENLDSE
                     ESVINGDIAGFAKRAGTKVGIAHTNLFRYIKKIGKDPLEYFKMLADNGIFWEMNVNYD
                     SIHGFRIHQYYLDFLKNEEMQDIVRKSGVEIGVGFDGHRVEDYLPERVVHMSNFLKEK
                     GIQIVDI"
     gene            5271..>6639
                     /locus_tag="PLI11_02400"
     CDS             5271..>6639
                     /locus_tag="PLI11_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ51745.1"
                     /translation="MLVKEKGMIYYKMEMLPEGSLKYQGVIDDSIRFIQENQLKDIQY
                     WKLFIKQFKLGNVDDQKLSWRGEYWGKMMRGASLTYIYTQDNELYDIIEKSVRDMLET
                     QDDLGRFSTYSVEKEFNEWDMWCRKYVMLGMFYFLDICRDGELKSRIVEAMTRHADYI
                     ISKIGPSEDGKLPITKTSIIWKALNSSSILEPFVRLYVITGYERYLDFSRHIIDCGFI
                     DGFNIIEACLENRLMPFEFPVTKAYEMMSCFEGLLWYYRVTGEKKYLDAAENFARNVE
                     KSDITVIGSAGCTHELFDNSKVNQFNPDFTGIMQETCVTVTWIKLCQLLFQVTGKKEY
                     LDYIEKSVYNALLGAVNTEKKSRDNSIPWFDSYSPLRMGVRGEKIGGYQKIDDTYYGC
                     CMAIAAAGTALAALSSVYRTEDGFYFAQYITGSIKTTTPKGRNIVFDVVSSYPFAGIV
                     KVEINM"
BASE COUNT         1951 a         1177 c         1316 g         2195 t
ORIGIN      
        1 acttcccaaa cagtatccat aatggttttt atgtgcctta gataatcatc tttagaaacc
       61 cttccgcttc catcattcat gccaaaagct atgaatacaa gatcgggttt gaattttgca
      121 gctctatcat atgcgtgttc cgcaccccag gaggagactg ttcctcccac tgctgtgttt
      181 attgattcta tatggtcgct tgcatatgcc tttttgagtg cctttactgt catcgtacac
      241 cagtcatcga tatgtggcat ctggtttatt actttgctgg aatttgcacc ggtggtaata
      301 ctgtcgccgt aaaccacaat cctcaatggt tctttgttct ttagtttgct aattgtaatt
      361 ggcagattga caccctggta gggaggaagg gggccttgcc atgtttcacc atgtatgtat
      421 gtcaccacgt attgcagttt atgcatggcg tctgcttctc caaagtatat gaatcctccg
      481 cctgttctgg gaaagcatcc tcctggaaca ttttcatcaa ggtaatagtc cttgtatttt
      541 accttttcaa tatttccatc tttaagaatt tccagtttcc catcttttag tatataatct
      601 gcaccttctt gataggttat tgtgtgtttg ctgttcttga ccgatatgat tttctctgcc
      661 ttgtaaagaa gaggaatggg gtcgattgac ccgtcctcct tttcaatagg catgaatgac
      721 tcatcgataa ccatgtttcc ttcccagaca ggtttaatgt atttctcaaa gtcgtacatg
      781 attggcctcc tatgctatat caatgtcatt ttcccgtttt tatctattat ggcaatggca
      841 aatgtatcca taaagacgat gtcgttttcg ttgtaaggtt taagtcctct catggttatt
      901 ctgaaggtaa tgccgtttgt ttcttttacg tccaagtaaa gaaacaattc ggaaccagca
      961 cttacatcaa aagtcttgct caaagtataa gtcaagacac cttcagcact cttcaaatcc
     1021 gctgttatat ccttctccca aacctccata ttatcaacag tggcaaatat gcgtgccatt
     1081 tcaatttcag tattttcaaa agccttgtat acaatattta ttatgttttc ctccaaggtc
     1141 agtttgttgt tcttaaacag tacgcgtcct tcaaccaaac cttttgcctt ttcggaaaat
     1201 atgcgtctag tatcaactct tctgtcaagg gtttcgattt tgcttacacc gtttgtcttt
     1261 acactgacta caacttcata agtttgaagt gcatccacgt atgttttggc taaaaacttg
     1321 ttgcttatct tttccgctat tacagtaata tcaccaatag ttacttccat ttcggaatca
     1381 tcagcaattt cttttggtac tacctcgaca atagcttcta ctgtaagatc actggcattt
     1441 atctctccaa gttttatatc atacgaagaa agaatgctgg cttgtttttt cagttcatgc
     1501 tgtatgttgc tgtttatgga actgatttca ctttgcagtg agtttacctt gttatttagc
     1561 tgcattattc tgttttcaag attcataatt gtgtttctta ccctgcgcaa tccaaccaaa
     1621 ctaaagatta gtaagaggat tagaaaaaac agggtaaagg acataagtaa tgtatttatc
     1681 ttttggttca tttaaattcc ctccttaatt caagtcttgg attgccatct tctgcatctc
     1741 aagttttgca cgcatctttt cgcggctgat tccgtatatc aaaagatata ttacagtagg
     1801 tatgtttgtg gttataagta ttgttatgag tccaattccg cttattatta cccttggtct
     1861 tgagtgcctg tcaaaggaaa cgtggccgtc aaccttttta aactcctcct tgatgtttcc
     1921 ttcgctgtca tatgtatagc gagtatctgg agttttaata ttgggaccaa agcgattatt
     1981 catacttaga aagtatgaca cccatattag cgataaaagt atgaatactg caggcagtat
     2041 gagtccaggc cacttgcttt tctttctaga caggtatgcc tgtaaaaaac acaagcctat
     2101 tgttatcgga attatgaaaa agaatattaa caataacatt attcattacc tctttccatt
     2161 ctttttttgt attcgttgat aagtagtttt gctgtctcaa aatcaatttt gtcgttaaca
     2221 gcaagccttt taataagtga aatatagggg tcgttttctc ttgattcact tatgcgtatt
     2281 ttctgtatta tcttgtctat cctaagccgc actgtggggt aggagacatt gtactggttt
     2341 gctatctctt tcaaggagcc ggatgccagt atgaagttcc gtatgaaatt aacgtcttct
     2401 tcttctagtt ctctcatcca ctcaggcact atgtctattg ccatattaaa cctcctattt
     2461 aattttatgt ttaacaatat taaatataca ctttaatttt attaaagtca atacttattt
     2521 tttatttact ttgattaaat ttttgtttgt ttggatataa tagtaatacg ttcagtcccc
     2581 tgccaaacat gaaacaaaat ctcacagctt gtgtgaggtt ttgttttttt gtatatttca
     2641 aaaaatgtat tgtggtataa tatatgtgta tttgtaatta ggaggatgta cacttgatct
     2701 ttattgccgg tattaacgat ggagaaaaga aacttaacta tagacaggaa atattgtgca
     2761 aggaatgcaa cagacagggt tttctagaaa tcattatgta ttccagatac ttgtctctgt
     2821 tctttatacc tgtattcaga tggaaaaagg aatattacgc caccaccact tgctgcaata
     2881 caatgtataa aattgatgat gaaatcatta aaaggctgaa aagaagggaa ataagccaca
     2941 tacctgacga ggagcttaag aggaattatt tctacgaaaa aacttgtgca aattgcaggt
     3001 atgtatctcc tcctgacttt gcatattgtc ctaagtgcgg aagcaggttg taaggtatgg
     3061 agtatttcga atatggtaaa gatgaagttg aatacttgaa gagcagggat aaggttttag
     3121 gcaaagctat ggattccgtg ggacatattg acagggaaat catacccgac ttgtttgaag
     3181 gtattgttta caatattgta gggcagcaga tttccatgaa ggctgcaagg actgtctggg
     3241 gaaggtttac aaataagttt ggcaaagtaa cccctgagac tttgtacaac caggacgttg
     3301 aagatattca aaagcttgga gtgtctttta gaaaggccgg atacataaag gaagtatcac
     3361 ggcaggtctg tgagaaggag ttggtgatag acgagttgta taagcttcct gatgatgagg
     3421 ttgtaaaaag actagtaaaa ctgcctggta ttggtgtatg gacctgtgag atgcttctta
     3481 tcttttcatt aaacaggatg aatgtactta gctggcagga ccttgccata aagaggggga
     3541 ttatcaatct ttatgggctt gatagccttg ataaggaaac tttcaatgag cttaaggaaa
     3601 ggtactcacc atatgcatcc gttgcatctt tgtatttatg ggaagttgcc aaaaataatg
     3661 gataaatctt gtaatgattc gtttaatggt atataatttg attaatacaa tttaggaggg
     3721 cggttatgga aaaagacagg cggtttgccg ttcttattga cgcagataat gtatcggaca
     3781 ggtacataaa attaatcctt gacgaggttt caaacgaagg cattctaaca tacaaaagga
     3841 tctatgctga ctggacaaaa ccttcattat catcatggaa gaacctgcta ctggcaaact
     3901 ccattactcc aatacagcaa tattcgtaca ccgccggcaa aattgctaca gactcagcga
     3961 tgataataga tgcaatggat attttgtatt ctgaaaatgt ggaaggcttt tgcatagtgt
     4021 ccagcaacag cgattttacc agactggcag taaggctaag agaagcgggc aagtatgtaa
     4081 ttggtatggg ggatagaaaa acaccccaag cttttgttgt agcctgcaac aggtttgtgt
     4141 ttctcgaagc cttggaaaag gacaaggccg gtgagattcc tttggaatcc gttacaagcc
     4201 tggatgttat aaaagatgcg ataaagcaga tagtggatga cacctccgat gaagaaggct
     4261 ggtgcccgct gagtgatgtt ggaaacactc ttcttaaaag atatcctgat tttgatgtca
     4321 ggaactatgg ttttacaaaa cttaccccgc ttataaagca ccttaactgt tttgaaatca
     4381 aatcggtggc agtaaatgaa aatattaaac tgatttatat tagaaacact gagtaagggg
     4441 attgtcagat atccactatt tgaattcctt tttccttaag aaaattggac atatgtacaa
     4501 ccctttcagg aagataatcc tcaaccctgt ggccgtcaaa tccaactcct atttccacac
     4561 ctgattttct tacaatatcc tgcatttcct cgttttttag aaaatccaga taatactggt
     4621 gtattcgaaa gccgtggatg gagtcgtagt ttacattcat ctcccagaag atgccgttgt
     4681 ctgctagcat tttgaaatac tcaagaggat cttttccgat cttttttatg tatctgaaca
     4741 gattggtatg ggcaatgcca acctttgtgc cggctctctt ggcaaagcct gcgatatccc
     4801 catttataac gctttcctca ctatcaagat tttcaacaag gcagtagtca aagcatgaga
     4861 tgtcctcatc gtcttttaaa tatagatctc ttcttaatgt gcatacctca attccgcaca
     4921 aaagcttgat tctgtccttg tagacttctt tcaacgaagt tagaagctcg taatattctc
     4981 tttttctatc tccaatccca tagttgtgat cacttattcc aaatacctca attccgccat
     5041 ctattgccgc ctgtataatc acttctggtt tgtctcttcc gcaggctgag taatatgtgt
     5101 gggaatgcaa atcgtgtatc atggtattct ccattctttt ttattataca taatacaagg
     5161 ttattttgaa aaactcaagt attgtttgtt gatactattt tacaaataat tgtgataaaa
     5221 atacaattat agtttaggcg accatggtat tgttttaagg aaggttaaca atgttagtaa
     5281 aggagaaagg catgatatat tacaagatgg aaatgctgcc cgaaggttca ttgaaatatc
     5341 agggtgtaat cgatgattcg atcagattta tacaggaaaa ccaattaaaa gacatacaat
     5401 attggaaact ttttatcaag cagttcaagc ttggaaacgt tgatgatcaa aaactgagct
     5461 ggcgtggaga atactgggga aagatgatga gaggcgcctc tcttacatat atttatactc
     5521 aggataatga attgtatgat attattgaaa aatcagtcag agacatgctt gaaacgcagg
     5581 atgatttggg caggttttcg acttattctg ttgaaaaaga atttaacgaa tgggatatgt
     5641 ggtgcaggaa gtatgttatg ctcggtatgt tttatttcct ggacatttgc cgtgatggtg
     5701 aattgaaatc aagaattgtg gaggcaatga caaggcacgc agattacata atatcaaaga
     5761 taggtccatc cgaagatgga aaattaccca taactaaaac cagtattata tggaaagctc
     5821 tcaactcaag ttcaattcta gagccctttg taaggcttta tgtaattaca gggtatgaaa
     5881 gatatctgga tttttcaaga catataatcg actgtggctt tatagacggg ttcaatatta
     5941 ttgaagcttg cttggaaaac agacttatgc cttttgagtt tcctgttaca aaagcatatg
     6001 agatgatgtc atgttttgaa ggtcttctat ggtattacag ggttacagga gagaagaaat
     6061 atcttgatgc tgctgaaaac tttgcaagaa atgtagagaa gtcagatatt acagtcatag
     6121 gttctgcagg ctgtacacat gaacttttcg ataattccaa ggtaaatcag tttaatcctg
     6181 actttaccgg tatcatgcag gaaacctgtg ttacagttac atggataaaa ttgtgtcaac
     6241 tgctttttca ggttacagga aaaaaagagt atttggacta tattgaaaaa tctgtgtaca
     6301 atgcgcttct cggagctgta aatacggaaa agaaatcaag agacaattcc ataccgtggt
     6361 ttgatagtta cagcccgctt aggatgggtg tacgcggtga aaaaataggt ggataccaaa
     6421 agattgatga tacatattat ggttgttgta tggccatagc tgcggcggga actgcattgg
     6481 ctgcattgtc aagtgtgtat agaactgaag atggattcta ttttgctcaa tatattactg
     6541 gaagtataaa gacaaccacg cccaaaggga gaaacatagt atttgatgtt gtatcatcgt
     6601 atccatttgc tggtatagta aaagttgaga taaatatgg
//
LOCUS       DAONVY010000053         6505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_8908_length_6505_cov_3.537829, whole genome shotgun sequence.
ACCESSION   DAONVY010000053 DAONVY010000000
VERSION     DAONVY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_6505_cov_3.537829"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLI11_02405"
     CDS             complement(<1..187)
                     /locus_tag="PLI11_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51746.1"
                     /translation="MKSGIEEEYEQSTQKNLHITEEKRKRLPIWQILIIDVLVAGLLI
                     NVFAFFHHVLPRTLDGGV"
     gene            273..635
                     /locus_tag="PLI11_02410"
     CDS             273..635
                     /locus_tag="PLI11_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPZ51747.1"
                     /translation="MKFCWCTLHVKNMDESVSFYKEVVGLKETRRFMAGPDTEIVFLG
                     NGETEVELIWDKNKESSDLGEDISLGFMVESVEKQITLLKEKGLQILTEPISPNPNIT
                     FFFALDPNGLKIQFVEKM"
     gene            complement(678..1325)
                     /locus_tag="PLI11_02415"
     CDS             complement(678..1325)
                     /locus_tag="PLI11_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ51748.1"
                     /translation="MKRAMGKYILAFVLVLVLVVLAGCGGSKDEEKKTPEPTAAPTDT
                     PEPTDTPEPTATPLPVVNVAEGKPVTADHEETSDFTADKAVDGDETTRWSGFSLALEN
                     PTENYDRWLIVDLENEYVINSFEIWFEFLAVSYKIEVSLTGEENSWTTVFDSDGINPV
                     GPNQVFEEDIEPVKTRYVKFSTYRHEDQAPHSGYPFCSIYELIVWGYDESELNTI"
     gene            1550..1918
                     /locus_tag="PLI11_02420"
     CDS             1550..1918
                     /locus_tag="PLI11_02420"
                     /inference="COORDINATES: protein motif:HMM:NF022171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HPZ51749.1"
                     /translation="MRHISALIIKFIMTAVILEIVLNIMTDLTFGQILWLSLITTVLS
                     YAIGDLIMLPLSNNTVASITDALIAFVVLYMANIWLDYTRVGIVDAIISALVLGVGEY
                     FFHKYVHRMIFSRDMRRMRS"
     gene            complement(1969..2109)
                     /locus_tag="PLI11_02425"
     CDS             complement(1969..2109)
                     /locus_tag="PLI11_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51750.1"
                     /translation="MDKRSKANIDFEDNDRITLRGRTPEEVERYLLSGEPDDLSYEYF
                     DE"
     gene            2222..3484
                     /locus_tag="PLI11_02430"
     CDS             2222..3484
                     /locus_tag="PLI11_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPZ51751.1"
                     /translation="MDYTVNNDTVEKIKDYIVENMPKATIYPEKEPVADNVYPLRFSY
                     TVPTVGQQFHYLFYWDTFFFTYALVHFGQYEQLKYNIENFFDLIDILGYVPNINIKSN
                     GDNRSQPPFLANMVDIYYEHSRDKNWLSRAISYIIREYNFWMTQRGFDNGLNHYGVQP
                     ESEENLLGFYDYVNTRVKLDAETKEEKLSVARNFFAEAESGWDFTPRFEHNAPKYAAV
                     DLNSNLYGYEVCISKYFKILGNESASKVWEAKANERKKKIQTMLFNKDKGFFMDYNVE
                     TGRFSPVKCAAGLFPAFYGIPTEKQMQTVVSSLSAIEFDYGISTCEDTDQKVVYQWDY
                     PNGWPCMQIIAVEALDRYGYKEEALRIAKKYLYNICVNFEKSGHLWEKYNVVTGTLDV
                     RDEYEMPAMLGWTAACFVFFLDYVNKKQ"
     gene            complement(3535..3861)
                     /locus_tag="PLI11_02435"
     CDS             complement(3535..3861)
                     /locus_tag="PLI11_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPZ51752.1"
                     /translation="MNVLFIVLNEVEHLDDVLDAFVEVGVTGATILDSQGMGSILTNG
                     HKSMKFYGTILNLLDNARPYNKTIFTVIEDDKVLEDAVNAVKEILDLDKPGVGLMFSI
                     PVGKVY"
     gene            complement(3880..5079)
                     /locus_tag="PLI11_02440"
     CDS             complement(3880..5079)
                     /locus_tag="PLI11_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPZ51753.1"
                     /translation="MLLKLGILVLVGIIGGRVANYFKFPSVTGYIVFGLLVGPSFLNL
                     VKHSDVDSLGIINDLTLAGIAFSIGNEFLLKDIKKVGKKIFIITAAEVIGALTIVFVL
                     MYYVLNQSFEFSIIMASLSASTAPAGVVMVMRELRADGPLTKTILPIVALDDAFGIMA
                     FGISLSLVHMLSGTKKVSVAAMFLNPILEIVGSLVLGLVMGAIMALVIKLARNNDEIL
                     VMTICAILLTYGLANLLNLSSLLTCMTLGGAFVNLKKNSQRVFKSISDFTPPINLLFF
                     TVAGASLKLDVLYTVGIVGVAYILARGTGKYLGATLGAVMVRSEPMITKNLGLGLLPQ
                     GGVSIGLSMMVKKELPHMSDSVVTIILFSVLIFEILGPITAKIAITRAGEDHSKELQK
                     GGAESAT"
     gene            5353..5772
                     /locus_tag="PLI11_02445"
     CDS             5353..5772
                     /locus_tag="PLI11_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HPZ51754.1"
                     /translation="MIWKDKYRLGVAEIDKQHMELFRRVNDFVVTLRSKGSWQEKVDK
                     VNDTLQFMQEYVVIHFKAEEAYQLKIGYTGYEHHKKLHDDMVAYVTEVAEEYEREGYK
                     EELIQRFAGKLLAWLINHVVAEDLKIAQYTIHKEEQK"
     gene            5907..6233
                     /locus_tag="PLI11_02450"
     CDS             5907..6233
                     /locus_tag="PLI11_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPZ51755.1"
                     /translation="MALLGDIKGTITFKFNADTALDLVEIMCGMEIDEVDEFVKSALG
                     EIANIISGNALTTLSQEEIIGDLEPPLIITQSKKYEENDNVHITVVDATSDIGTMNFV
                     FVEKDQ"
     gene            complement(6227..>6505)
                     /locus_tag="PLI11_02455"
     CDS             complement(6227..>6505)
                     /locus_tag="PLI11_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016203555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPZ51756.1"
                     /translation="GFVYYMFNKHGVKMPRQSSASLFSQGTSVSKSNLRLGDLVFFAV
                     NSTGTISHVGFYLGDNEWISATTSKGIAVYSMDNTYWSKYYVGAKRVY"
BASE COUNT         1992 a         1368 c         1243 g         1902 t
ORIGIN      
        1 tcactcctcc atccagtgtt ctgggtagta catggtgaaa aaatgcaaat acattaatta
       61 ataagcctgc tacaagaaca tcaataatga ggatttgcca tatcggcaaa cgttttcttt
      121 tctcttctgt aatatgtagg ttcttttgtg ttgattgttc gtattcttct tctataccgc
      181 ttttcattaa ccctcgcctc ggttcatgtt ataattgtgg tgaaaattat tatactaaaa
      241 ctagcagcaa gcaatcactg gaggtgtttt aaatgaaatt ctgctggtgc actttacatg
      301 taaaaaatat ggacgagtct gtcagtttct acaaggaagt cgttggactt aaagaaacaa
      361 gacgattcat ggcaggtcca gatacagaaa tcgttttctt gggcaatgga gaaaccgaag
      421 ttgaacttat ttgggacaaa aacaaagaat ccagcgattt gggcgaagat atctcactgg
      481 gtttcatggt agagtcagtc gaaaagcaaa ttacgctatt aaaggaaaag ggtttgcaaa
      541 tccttaccga acccatttca cccaatccca acataacatt cttctttgcc ctggatccaa
      601 acggtcttaa gattcagttt gttgaaaaaa tgtagcaaaa tagaggcgct ctcatttcga
      661 gactgcctct gtttttacta aatcgtattc aattcgcttt catcataccc ccatactatc
      721 agttcataaa tgctgcagaa ggggtatcca gaatgtggtg cttgatcttc atgccggtat
      781 gttgagaact ttacatatct cgttttcaca ggctcgatat cctcttcaaa cacctggttt
      841 ggacctaccg gattaattcc atcagaatca aaaacagttg tccatgagtt ttcctctccc
      901 gtcaacgaaa cttcaatttt gtagctgaca gccagaaact caaaccatat ttcaaaggaa
      961 ttgataacgt actcgttctc caaatcaaca atcagccaac gatcatagtt ttccgtagga
     1021 ttctccagcg caaggctgaa accgctccat cttgtcgttt catcaccatc caccgccttg
     1081 tctgcagtaa agtcagaagt ctcttcatgg tctgctgtaa ctggtttgcc ttccgccaca
     1141 tttaccactg gcaatggagt tgctgttggt tcaggtgtgt cggtaggttc aggcgtatct
     1201 gttggagccg ccgttggctc aggagttttc ttttcttcgt ccttggaacc tccgcagcct
     1261 gccagcacaa ccagtaccag aacaagtaca aaggcaagta tgtacttacc cattgctctt
     1321 ttcaattttt tcatccccct ttttttcgtc gcttgctctt ctccacggaa gatattaatt
     1381 tcaactttaa ttatacagca taaattgaat aatttatgtc tatattgtta acactagatt
     1441 gctgatatgc ggaaaatact tttatcggaa taattaatcc aatgaacaaa attttccttg
     1501 tttatctaat ggacaattag aacatactac tgtcaaagga gtgagtattg tgagacacat
     1561 tagtgctttg attataaaat tcataatgac agctgtaatt ttggaaattg tattgaacat
     1621 catgaccgat ctaacattcg ggcaaatact ctggctgtca cttattacta ctgttttgtc
     1681 atacgcaatt ggcgatttga taatgcttcc tctttcaaac aacaccgttg cttccataac
     1741 agatgctttg atagcatttg tcgtcctgta catggccaac atatggcttg actatacccg
     1801 ggtgggaata gtcgacgcaa ttataagcgc attggtactg ggagtaggtg aatacttctt
     1861 ccacaaatat gttcatagaa tgatcttctc cagagacatg cgtcgaatgc gttcctgaat
     1921 caatcttttt gtaaaaaaac cgcccattta gcgggcggtt tttttgttct actcatcaaa
     1981 atattcgtaa gataaatcat caggctcgcc tgacaaaaga tatctttcca cttcttcagg
     2041 tgttctgcct cttaatgtaa ttctatcatt gtcttcaaag tcaatgtttg cttttgatct
     2101 cttatccatt agtttcacac tccttttcat gagtatgcat atattattcc aattttattg
     2161 aaaataaaac atgcgtggta tacaattgtg tcaacgaaaa cgatggagaa gaggtgtttt
     2221 tatggattat acggttaaca atgatactgt agagaaaata aaagactata tagtcgaaaa
     2281 catgcccaag gcgacgatat acccggagaa ggaacctgtg gctgataatg tatatccttt
     2341 gcgtttttcc tatacagttc ccactgtcgg gcagcagttc cactacctgt tctactggga
     2401 tacttttttc tttacatacg cattagtaca ttttggccag tacgagcagc tgaaatacaa
     2461 catcgagaac tttttcgatc tcatagacat ccttggctat gtacctaata taaatattaa
     2521 aagtaatggg gacaaccgct cgcaacctcc attcctggca aacatggtgg atatatacta
     2581 tgagcattca agagacaaga actggctttc aagagctatt tcatacataa tcagggagta
     2641 caatttctgg atgactcaaa ggggttttga caacggtctc aaccactacg gtgttcagcc
     2701 cgagagcgaa gaaaaccttt taggtttcta cgactatgta aatacccggg taaaattgga
     2761 tgcagaaaca aaagaagaaa agcttagtgt tgcaagaaac ttttttgccg aagccgagtc
     2821 tggatgggat tttacccccc gatttgagca taacgcacca aaatacgcag ctgtggattt
     2881 aaacagcaat ctgtacggat atgaagtatg catttcaaaa tatttcaaaa tccttggcaa
     2941 tgagtcggca tccaaagtct gggaagcaaa agccaacgaa agaaagaaaa agattcaaac
     3001 catgcttttt aacaaagaca aaggtttctt catggactat aatgtggaaa ccggcaggtt
     3061 ttctcctgtg aagtgtgcag cgggcttgtt ccccgccttc tatggaatac ccacggaaaa
     3121 acagatgcaa accgtagtct ctagtctgag cgccatagaa ttcgattatg gtatatccac
     3181 ttgtgaagat acggaccaaa aggttgtcta ccagtgggat tatcccaacg gctggccctg
     3241 catgcagatt attgcggtcg aagctcttga caggtacggc tacaaggaag aggcactccg
     3301 gatagcaaag aagtatttat acaacatatg cgttaatttt gaaaaatcag gtcatctttg
     3361 ggaaaaatac aatgtcgtta caggaacact tgatgttcga gatgaatacg aaatgcctgc
     3421 catgctgggc tggaccgcag catgtttcgt attctttttg gattatgtca ataaaaaaca
     3481 gtgagcaata aatacaagaa cgcaccctaa tcattttcga ttgggtgcgt tttcttaata
     3541 gactttcccc acaggtatgg agaacatgag cccaacacca ggcttgtcaa gatcaagtat
     3601 ttcctttact gcattgacgg cgtcttccaa caccttgtca tcttcaataa cagtgaaaat
     3661 ggttttgttg tacggtcttg cattgtccaa caagttaagt atagttccat aaaacttcat
     3721 gcttttatgt ccgttggtta aaatgctgcc cataccctgg ctgtccagta ttgtcgcacc
     3781 agtaacgccc acttcaacaa aagcatccaa tacgtcatcc aggtgctcca cttcatttaa
     3841 tacgataaac agaacattca tactctcacc tacctttaac taggtcgcag actccgctcc
     3901 gcccttttgt agttccttgc tgtggtcttc tcccgctctt gttatggcaa ttttagcggt
     3961 tattggccct aatatttcaa atatcaagac actgaacagt ataattgtta caaccgaatc
     4021 actcatgtga ggaagctctt tttttaccat catcgagagt ccgattgaca ctccgccttg
     4081 aggcagcaag cccagtccca aattctttgt aatcataggc tcggagcgta ccattacagc
     4141 tccaagagtt gcaccaagat attttcctgt gcctcttgct agaatatatg caactcctac
     4201 tataccaact gtgtacaaaa cgtcaagctt taaactggca cctgcgactg taaagaacaa
     4261 tagattaatt ggcggtgtaa agtcactaat ggatttaaat accctctggg agtttttctt
     4321 caggtttaca aaagctcctc ccaaagtcat gcacgtaagc aatgaggata gattcaaaag
     4381 gtttgccaat ccgtatgtaa gaaggatggc acatattgtc ataaccaata tctcgtcatt
     4441 gttccgcgcc aattttataa ccaaagccat tattgcaccc atcacaagac caagcactag
     4501 cgaccctacg atttccaata tagggtttaa gaacatggct gcaacagaca ctttctttgt
     4561 tcctgaaagc atatgcacaa gagacaagct aattccaaac gccataattc cgaaggcatc
     4621 gtcgagcgca acaatgggca ggattgtttt tgtcaagggt ccatcagccc ttaactccct
     4681 catcaccatt acaacacccg ctggcgctgt tgatgcggac aagctcgcca tgataatgct
     4741 gaattcaaat gattggttca gtacataata cattaacaca aatacgattg tcaatgcacc
     4801 aatcacttca gctgcagtga taatgaatat tttctttcca acctttttta tatctttcaa
     4861 taaaaactca tttcctatgg agaaagcgat gccagcaaga gtaagatcat ttatgatgcc
     4921 tagcgaatct acatctgaat gtttaacaag gtttaaaaac gatggtccaa caagcaaccc
     4981 gaaaacaatg tatcctgtta cacttggaaa cttaaaatag ttcgccaccc ttcccccaat
     5041 aatccccacc agcactaaaa ttccaagttt taatagcata acctattaac aatccctcct
     5101 tactatattc ttttgtcatt atggagtatc cagcaatgct tttttcttgc ttccacaata
     5161 catccacaaa gcataattat atcacattca agatgctttt accatagttt gttcttttgt
     5221 atgcaagaat acattaagca aacaaaaacc gggagtagaa agtacaagag tttcgtaaga
     5281 gcggattttc ttgttgaaat gacaggaaga gtttgctatc atattgacat tatatttaaa
     5341 aaggagattg tcatgatttg gaaggataag tacagactag gtgttgccga aattgacaaa
     5401 caacatatgg aacttttcag acgtgtaaat gactttgttg taacgcttcg ttccaaaggt
     5461 tcatggcagg aaaaagtaga caaagtcaac gatacattgc aattcatgca ggagtatgta
     5521 gtgattcact tcaaggccga agaagcttac caactgaaga ttggctacac cggatacgag
     5581 catcataaaa aacttcatga tgatatggtt gcgtacgtaa ccgaagtagc agaggagtat
     5641 gagcgtgaag gttacaaaga ggaactcata caaaggtttg cgggaaagct tcttgcatgg
     5701 cttatcaacc atgtagtggc tgaagacctc aaaattgcac aatacacaat acataaggag
     5761 gaacagaagt gaacaacaaa atacttgaac attttcttca ggctacgaac gaagtatttc
     5821 aaatgatgtt cgatatcgag tttgtttttt ccacacctat ggaagccaaa tccctgcatt
     5881 atgattaagg ccatatttgc gctgagatag ctcttttggg cgatattaaa ggaacaataa
     5941 cctttaaatt taatgcagat acagctcttg atctcgttga aatcatgtgc ggaatggaaa
     6001 tcgacgaagt agacgagttt gtaaaatcag ccctggggga aattgcaaat attatcagcg
     6061 gaaacgccct tacaacattg tctcaggaag aaatcattgg agacctggag cctccgctga
     6121 taataacgca gagtaagaaa tatgaagaga acgataatgt acatatcact gttgttgacg
     6181 caacatctga tattggtacc atgaatttcg tgtttgttga gaaagatcaa tagactcttt
     6241 ttgcccctac gtaatatttt gaccaatagg tattatccat agagtaaacg gcaatacctt
     6301 tcgaggttgt tgcggatatc cactcattat ctcccagata aaaaccgacg tgcgaaatag
     6361 tgcctgtgga gttgaccgca aaaaaaacaa ggtcacccag acgcaaattg cttttggaga
     6421 cactggtacc ctgggagaac aaactggcag acgactgtct gggcattttt acaccatgtt
     6481 tgttgaacat atagtaaaca aaacc
//
LOCUS       DAONVY010000054         6431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9047_length_6431_cov_3.353827, whole genome shotgun sequence.
ACCESSION   DAONVY010000054 DAONVY010000000
VERSION     DAONVY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_6431_cov_3.353827"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PLI11_02460"
     CDS             complement(<1..1330)
                     /locus_tag="PLI11_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51757.1"
                     /translation="MKKLCFALTVILVGAIFTIIGFNAEEVGEDMDLFIEPEKVVQTR
                     LTGLCVQFNATSSFSKIDIFIANASVNRNCIVEFKLYKWDENIAKTVNGKPVHCEKRD
                     EFFPRNTYYTWDLSSVMPKKGEYLFVMEKMGGNGFLDVNIMYPEKENVRLYENFSERF
                     GSLKMRINTKGLDKLSDNSIELFDGFDTWVATDGLGREVPSTQISGKVRNDKYVGLFF
                     HTWHTHNHAKGMRNITEILKEHPEIVHDWDSEHWGTGGVFFWNEPIYGYYRTDDRWVL
                     RKQAEMLANAGVDAIFFDNTNNEFTFVEEVLVLLEVFAKARLDGVKTPQISFILPIFD
                     YDFVAIQLRELYREIYSQGRYKDLWFYWKGKPLIMCYPGWLNPESDPIDAEIMEFFTF
                     RPPNHSHTWDNVQVRDENGNPLIYGAIQPEIKNNYIIWNWISIYPQLIARN"
     gene            complement(1327..2157)
                     /locus_tag="PLI11_02465"
     CDS             complement(1327..2157)
                     /locus_tag="PLI11_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51758.1"
                     /translation="MKRFIVLSMFFVVLCSFIGSFSVMASEAIIFDFSIEPNYEIVAT
                     DPEQGDNWNIYWDDGLSFLGKYNLTRVSFSYDKNEKACVFTAHTQTGVMDPYIVAAQP
                     GTNTGADMPEKYHIDTSKYKYIKVMYKANSDTASTSSFHWISTNVPAYDDGNAYLYSI
                     TANNEWQEITLDMNGVLAWEYAGVITQLRIGTYRSGTISDKDTMAYKYIGFFKTREEA
                     DAYSRLDTRTPVPQPTENTGKEKDAKGNDLYILFIILAVIVVVMVGVFFVRSKRDKES
                     "
     gene            complement(2163..3053)
                     /locus_tag="PLI11_02470"
     CDS             complement(2163..3053)
                     /locus_tag="PLI11_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPZ51759.1"
                     /translation="MDKGLISNIQHFSLGDGPGIRTTVFMQGCNLLCPWCHNPETISQ
                     KPRLMFHKNLCAKCRLCEIVCTRGVHSIKDGEHVVEVDKCDFCGMCEKTCTTKALKLS
                     GRYMTVDEVFDTLYKDYEYMQESGGGVTISGGEPLLQASFVSALAEKLSKNNIHVIID
                     TAGNVPFSLFEKVIPFTNTFYFDIKGNNEVYDMLKGDYELVCSNLHKLVQSGCDVVVR
                     IPIIPGINDRVDVIKEVEKMLGVTGVEKVSLLPYHNLGISKYFALCEDYSLSDLKEPS
                     EEIMKDIANLFINCHVDFER"
     gene            complement(3046..5205)
                     /locus_tag="PLI11_02475"
     CDS             complement(3046..5205)
                     /locus_tag="PLI11_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPZ51760.1"
                     /translation="MLTPLERLKKEINEKWPQAARRVTPYYMFEGYAEHFDSEVPIAR
                     AYAVASVFRKHRKHVYDNDMIAGSIRGAIEDESVISNTALQHAQNVCANYGSRHFGTN
                     FDHYAPDYETFLSDGIKGTLEKIEKSKLVHKGDKKKQVFLEACEISMQAFSDMVRGYS
                     DKALERAEAFEKGSCEYENLMKVYETCKKVSYFKPETFREALQLVWFVHTAFLYEGRY
                     AMALGRLDQYLYPYYKKDIEEGRINDKEALDLLSCTLYKIYECRYFGGDDVVNIAIGG
                     VLPNGLGGVNELSYLILKAVEICNVPGPNLSARIYSGIPEGFLDECLKVIGTGLGYPA
                     LMNDDVNVPALLRHGYSIEDARNYCMVGCIENFIPGKQPPWSDGRFNSPKYIELVLNN
                     GVCMHTGVRLGPSTGDVSELKTMDDFLHALRKQLEHGAAMYVRMFKNENERYNTYNYM
                     QPFLSCFCYPCIERGLDINDGGALYPSVHGAACMGIGTFADSLAAVEMFVYNKREIDL
                     SLLKEAILNNFEGYEEVLAKVLSAPKYGNDDDFVDKYAKWFVNVHYEIFSKYKTHDGG
                     AFYIAIAANISNIYAGMEIAATPDGRRDGEPLSDAASPVYGRDVHGPTLALKSLTKPD
                     YTKVSCGTVVNQKYTRNMFTDPVKRKKLLDMIKVYLKNGGQEIQINAVSKEILIDAMK
                     NPENYTDLVVRVSGFSAYYNSLSNEVKLDILRRTEHG"
     gene            complement(5211..6296)
                     /locus_tag="PLI11_02480"
     CDS             complement(5211..6296)
                     /locus_tag="PLI11_02480"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPZ51761.1"
                     /translation="MPKAAVYQGANKEFEIREFPLTKPQKDMALLRLVASGVCGTDLH
                     ILNGKLGIEPPKVIGHEFVGEVVELGSESDTLNVGDNVIATIAVPCEECLLCKTGDDA
                     NCVNMGVTNGGNPYDEPHFWGGFGEYNYSPIKNLVKIPKSINPKTASVFACAGSTVIH
                     AFSLAKRANVDLKNLKTCVVQGLGPVGMFAVAYLASLKVENVIAITGRENKKREELAK
                     SFGSTKIFSLEKNTFEEIDGYIKSVSGGLGADLAVEASGNKDAFLQGIDMLRNRGVYL
                     VPGQYSNSGTIPLPPQVITFKALHIIGSSQYSAVDIEEYVKFLEENRDLHPVIDSMIT
                     EYLLSDINKAFENAKQGKNVKTILVGG"
     gene            complement(6332..>6431)
                     /locus_tag="PLI11_02485"
     CDS             complement(6332..>6431)
                     /locus_tag="PLI11_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014114947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HPZ51762.1"
                     /translation="VEKGKLVLGTWQGIYFCEFDGPRTRNFYVKVM"
BASE COUNT         1822 a         1617 c         1060 g         1932 t
ORIGIN      
        1 gatttcttgc aattaactga ggatatatcg aaatccagtt ccagattatg tagttgttct
       61 taatctccgg ttgaatcgct ccgtaaatca aaggatttcc gttttcgtcc cttacctgca
      121 cattgtccca cgtatggctg tggtttggcg gtctgaaagt gaaaaactcc ataatctctg
      181 catcaatcgg atcggattct ggattcaacc acccaggata gcacataatc aaaggcttcc
      241 ccttccagta gaaccataaa tccttgtatc ttccttgaga atagatctcc ctgtacagtt
      301 ctcttagctg tattgccaca aaatcatagt cgaatatggg aaggataaag gaaatctgtg
      361 gagtcttgac tccatccaat ctcgcttttg caaagacttc aagaagaacc aggacctctt
      421 ccacaaaggt aaactcgttg tttgtattgt caaaaaagat ggcatccaca cctgcattgg
      481 ccagcatctc tgcttgtttt cttaataccc atctgtcatc cgtcctgtaa tatccgtaaa
      541 tcggctcatt ccagaagaaa actcctcctg ttccccaatg ctcactgtcc cagtcatgaa
      601 caatttcagg atgttccttc agtatttccg taatatttct cattcctttg gcatggttgt
      661 gggtatgcca ggtatggaaa aacaatccca catatttgtc atttcgcacc ttgcctgaaa
      721 tttgcgttga tggtacttcc cttcccaagc catccgtcgc cacccatgtg tcaaacccgt
      781 cgaacaactc aatggaattg tccgacagtt tgtcaagacc ttttgtgttt atacgcatct
      841 taagacttcc aaaacgctcg ctaaaattct catacagcct tacattttcc ttctctggat
      901 acatgatatt tacatcaaga aaaccattac cacccatctt ctccataaca aacagatact
      961 cacctttttt aggcataacg gaagataaat cccatgtata gtatgtattc ctgggaaaaa
     1021 actcgtccct tttctcacaa tggacaggct tgccgtttac ggtctttgca atattttcat
     1081 cccatttata cagtttgaac tcaacaatgc aatttcgatt aacagacgca ttggctataa
     1141 atatatcaat ttttgaaaaa cttgaagttg cattgaattg aacgcacaac ccagtaagcc
     1201 tggtttggac caccttttca ggctctatga acaaatccat atcctcacca acttcctcag
     1261 cattaaaccc gataattgta aatatcgcac caacaagaat aaccgttaaa gcaaaacata
     1321 actttttcat gactctttat cccgtttgct tcttacaaag aaaacgccca ccatcacaac
     1381 cactattacc gccagaataa tgaaaagaat atacaggtcg tttccttttg catccttttc
     1441 cttcccggta ttctcagttg gctgtggcac aggagttctg gtatcaagtc ttgaataggc
     1501 atccgcttct tccctggttt taaagaaacc aatatacttg tacgccattg tatccttgtc
     1561 gctgattgtc ccactcctgt atgtaccaat tctaagttgt gttatgacac ctgcatactc
     1621 ccaggcaagc acgccattca tgtcaagggt aatctcttgc cactcattat tcgcagtaat
     1681 actatacaaa tatgcattgc catcgtcata agctggaaca ttggtgctga tccagtggaa
     1741 ggaagaagtg ctggcagtat cagaatttgc cttgtacatg acttttatgt acttgtactt
     1801 gcttgtatct atatgatact tctctggcat gtcagctcct gtattcgtac ctggctgggc
     1861 tgcaactata tacggatcca ttaccccggt ctgggtatgg gcggtgaaga cgcatgcctt
     1921 ctcattcttg tcatatgaaa aggaaaccct ggtaagatta tatttcccta gaaaactcag
     1981 accatcatcc caatatatgt tccaattgtc tccttgttct ggatcagttg caacaatctc
     2041 atagttgggc tcaatagaaa aatcaaaaat tattgcctct gatgccataa cactaaacga
     2101 accgataaaa gaacacaaaa caacaaaaaa catggataaa actatgaatc gtttcatgaa
     2161 acctacctct caaaatcaac atggcaattt ataaaaagat tggcgatatc cttcattatc
     2221 tcctcgcttg gttctttcaa atcagacaag gaataatcct cacacaaagc aaaatacttg
     2281 gaaattccca aattgtgata tggcagcaaa gaaaccttct caacacctgt tacaccaagc
     2341 attttttcca cttccttaat tacatcgacc ctgtcattaa ttcccggtat gattggtatc
     2401 cttaccacca catcgcatcc cgactgtaca agcttatgca aattgctgca tacaagctca
     2461 taatctccct taagcatatc atacacttca ttgttgcctt ttatgtcgaa ataaaaggtg
     2521 ttggtaaagg gaatcacctt ctcaaaaaga gaaaacggca cattaccagc cgtatctata
     2581 atcacatgaa tattattttt acttaacttt tctgccaatg cggatacaaa agacgcctga
     2641 agcaaaggct ctccacccga tattgtaact ccaccaccgg actcctgcat gtattcataa
     2701 tccttgtaga gagtatcaaa cacttcatcc acggtcatgt atctgcctga aagtttcaaa
     2761 gcttttgttg tacatgtctt ttcacacatg ccacaaaagt cacacttgtc aacttccaca
     2821 acatgttcac catccttaat agagtgaaca cctcttgtgc agacaatttc acaaagccta
     2881 cattttgcac atagattttt atgaaacatc agccttggct tttgggatat ggtctcggga
     2941 ttatgacacc aggggcacaa aagattgcac ccctgcataa acacagttgt ccttatgccc
     3001 ggaccgtctc ctaatgaaaa atgctgtata ttgcttatca aacccttatc catgttcagt
     3061 ccttcttaat atgtcaagct ttacttcgtt cgacaaagaa ttgtagtatg cactgaatcc
     3121 cgacaccctt accaccagat cagtataatt ctcaggattc ttcattgcat caataagtat
     3181 ttcttttgat accgcattta tctgaatctc ctgaccgcca ttctttaaat acacctttat
     3241 catgtcaagc agtttcttcc ttttcaccgg atccgtgaac atattccttg tgtacttctg
     3301 attaacaacc gttccacagg acactttggt gtaatccggc ttggtaaggg actttagagc
     3361 caatgtagga ccatgcacat ctcttccata tacgggagag gcagcatcac taagaggttc
     3421 tccatccctt cttccatcgg gagtagctgc aatctccata ccagcatata tattactaat
     3481 atttgcagca atggcaatat agaaggcacc gccatcatgg gttttgtatt tggagaatat
     3541 ctcataatgc acatttacga accacttggc gtatttgtct acaaaatcat catcgtttcc
     3601 atatttggga gctgacaaaa cctttgccaa aacttcttca tatccttcaa aattattcag
     3661 tattgcttct ttaagaagcg aaaggtcaat ctcccttttg ttgtaaacaa acatctcaac
     3721 agcggccaaa gagtctgcaa aggttccaat gcccatgcat gcagcaccat ggacagacgg
     3781 atacaaagct ccgccatcat ttatgtcaag acctctttca atacacggat agcagaaaca
     3841 ggacaaaaac ggctgcatgt agttgtatgt attgtatctt tcattttcat tcttgaacat
     3901 tctgacatac atggccgcac cgtgttccaa ctgctttctc aatgcatgaa gaaaatcatc
     3961 catggtcttc aactcggaaa catcccccgt tgaaggccca aggcgcacgc ccgtatgcat
     4021 gcacacccca ttgttaagaa caagctctat atacttgggt gagttgaatc ttccatccga
     4081 ccagggcggt tgtttgcctg ggatgaagtt ttctatgcag cctaccatgc agtagtttct
     4141 agcatcctct atactgtaac catgtcttag aagggctggc acattcacat cgtcattcat
     4201 aagcgctgga tatcccaaac ccgttccaat aaccttaagg cattcatcaa gaaaaccctc
     4261 cggtatgccg gaatatattc tggcagaaag attcggtcct ggcacattgc atatctccac
     4321 cgccttgagt atgaggtagg aaagctcatt gacaccaccc aatccattgg gcaaaactcc
     4381 gccaatggca atattcacca catcatctcc accaaaatag cgacattcgt agattttata
     4441 caatgtgcag cttaagaggt ccaaggcttc cttgtcattt atcctgcctt cttcaatatc
     4501 ttttttgtag tatgggtaaa gatactgatc cagccttccc aaagccattg catatctgcc
     4561 ctcatacaaa aaagccgtgt gaacaaacca tacaagctga agagcctcac gaaaagtttc
     4621 gggtttgaaa tatgagacct ttttacaggt ttcgtaaacc ttcataaggt tttcatactc
     4681 gcagcttcct ttttcaaaag cttctgctct ttccaaagcc ttgtccgaat accctcttac
     4741 catgtcggaa aaagcctgca tggaaatctc gcacgcttca agaaacactt gcttcttttt
     4801 atcgccttta tgtaccagtt ttgatttctc tatcttctcc aatgtgcctt ttataccatc
     4861 actcaaaaac gtctcataat caggtgcata atggtcaaaa ttcgtaccaa aatgcctcga
     4921 gccgtaattt gcacacacat tctgtgcatg ctgaagcgcc gtatttgata tgacactttc
     4981 atcttcaatg gcgcctctta tgctgcctgc aatcatgtca ttatcatata cgtgttttct
     5041 gtgttttctg aacacagagg caactgcata cgctcttgca atcggcacct cggaatcaaa
     5101 atgctccgca tagccttcaa acatgtaata aggggttacc cgccttgcag cctgcggcca
     5161 tttctcgtta atttcttttt tcaacctctc aagaggcgtg agcatataaa tcacccgcca
     5221 accagtatcg tcttcacatt tttgccctgt tttgcattct caaaagcctt gttgatatcg
     5281 gaaagcagat attccgtaat cattgaatca ataacagggt gcaaatctct gttttcttca
     5341 aggaatttta catactcttc aatatccaca gcagaatact gggacgaacc aataatatgc
     5401 agtgccttga aagttataac ctggggtgga agcggtattg ttcctgagtt ggaatactgc
     5461 cctggaacca ggtaaactcc tctgtttctt aacatgtcaa taccttgcaa aaaggcatcc
     5521 ttgttgccgc tggcttccac agccaaatca gcaccaagac caccgcttac acttttaata
     5581 tatccatcta tctcttcaaa ggtattcttc tcaagactga aaatctttgt agatccaaag
     5641 ctttttgcca gctcttctct ctttttattc tccctgcctg ttatagcaat tacattttcc
     5701 accttcaaac tggcaaggta agcaactgca aacattccaa caggccccaa tccctgaacc
     5761 acgcaagttt tcaagttctt caaatccaca ttcgcccttt ttgccagtga aaacgcatgt
     5821 attacagtag agcctgcaca ggcaaaaaca ctggcagtct tgggattaat gctttttggt
     5881 atctttacaa gattctttat aggtgaatag ttgtattctc caaaaccgcc ccagaaatga
     5941 ggttcatcgt aaggatttcc accatttgtc acacccatgt ttacacagtt ggcatcatct
     6001 cccgtcttgc agagaaggca ctcttcgcat ggtactgcaa tggtggcaat tacattatca
     6061 cctacattta gagtatcact ttccgagccc agttccacca cttctcccac aaactcatgc
     6121 ccaattactt taggcggctc tattccaagc tttccgttta atatatgcaa atccgttcca
     6181 caaacaccgc tggccacaag gcgcaaaagc gccatgtcct tctgtggttt tgtcagtgga
     6241 aactctctaa tttcaaactc cttgttcgct ccttgatata ctgccgcttt tggcacttaa
     6301 aacacctcca attacattac ctttacatca attacattac ctttacataa aaattccttg
     6361 ttctgggtcc gtcaaactca cagaaatata taccctgcca tgtgccaagc acaagttttc
     6421 ccttttccac a
//
LOCUS       DAONVY010000055         6387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9118_length_6387_cov_3.765635, whole genome shotgun sequence.
ACCESSION   DAONVY010000055 DAONVY010000000
VERSION     DAONVY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9118_length_6387_cov_3.765635"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLI11_02490"
     CDS             <1..427
                     /locus_tag="PLI11_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51763.1"
                     /translation="ECDLFFDKPLLEKVVRGEGDCSILVSPYNRATMDGTVIRTDGDK
                     AKALIIKKHQIKDFDYSGYMKTVNVYKFKKDFMLNHFLPAVSTYVQYESVNSYYELVL
                     GSLIYYGNSDIRVVMIEEDAWAEIDNAEDLARAEKKFKR"
     gene            433..1143
                     /locus_tag="PLI11_02495"
     CDS             433..1143
                     /locus_tag="PLI11_02495"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPZ51764.1"
                     /translation="MYKDVIGIVGGMGSFATCDLFRRLIEAFPAEKEWDRPRIVIDNN
                     CTMPSRVRAIIYKEERPKLVELLTESVINLRNMGCTKIILACNTSHVFLEEIYELYEK
                     KTGNQNCNIMNMIEIIAESLHAQGIKKVALMATEGTMDSKIYDEYLSKYGIEVIPPKK
                     EDYVEFRYLIECVKQNKVDEEAVNRFINSAENLGCRDVILGCTEFPVFLRYMSRMPNV
                     NLHDPLEYLITKLKKELM"
     gene            1148..2326
                     /locus_tag="PLI11_02500"
     CDS             1148..2326
                     /locus_tag="PLI11_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPZ51765.1"
                     /translation="MKIYDKLLNHYQVFFNKRIKYLRRKRNILLYCASDTMVWHILNY
                     YDQVKENPVYNFYLYYGDAKTDNETRTRRTYEITKGTRIEPITNKKRLVLVPLDLIVT
                     ADLDYEPLRYTKDIVPILYVNHGFHIVSIDNDRNLYAYGERALDDKGRCKFSCILEAN
                     MRIANVMNKREPFKGLVMHVGAKDGDIIEREVDNYSMYRKKLGIEEEETLVCVFGTWR
                     KESLFQYLGKEFLEAMGSMTDKGYKFVLSIHPREYVKYDEDVEPMGHVLEEYAQKYGF
                     IVRHPKDSYIPYLIASDIVISDYTSLYENAILAGKKLCLSYFPETSVGDLSIANFLRN
                     EIKVLRREDIKELGWHLNEIKVADFSDILEKYRKEIYPGIGVYKNRVKEITRKLVGLK
                     "
     gene            2323..3297
                     /locus_tag="PLI11_02505"
     CDS             2323..3297
                     /locus_tag="PLI11_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPZ51766.1"
                     /translation="MKRELVSVIVPVYNTEKYLKKCIDSILNQTYKEIELILVDDGST
                     DNSLEILREYERQDTRVFVIPQENKGVSGARNRGLEAARGKYIGFVDSDDYISLYMIE
                     YLVGLLEEGNDFASCGWIYETEEGEREQSTSGSIKEMETRNDILKCMFEQGGCAPNVC
                     NKLLRGDVVREHGIRFPEGIKIGEDMIMLCQYVLNCEKGVYDPNPLYHYVTRKDSAYH
                     KRIVKGSFDESFVNEIKAHEIVLEMLPDCGARKSFINKLYYVNLRTMMFLAHAGFPIR
                     ELYDYLIRFLRSNLTGYLSSDRGLKQKIHALLMCTSPRLWHYIFKDRG"
     gene            3302..4882
                     /locus_tag="PLI11_02510"
     CDS             3302..4882
                     /locus_tag="PLI11_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar translocase"
                     /protein_id="HPZ51767.1"
                     /translation="MAARKSRSLYSTKNIITGIGGQIINIVLGFVARTFFINALGQEY
                     LGVSSLFTTILTMLNISELGISTAIVFSLYKPLAEKDYEKCQILLKLLKKIYAVIGIV
                     VLGIGLSIMPFLPYIIKGNTEVANIKVIYIIYLLQSALSYWFYSYKYTILQADQRQYV
                     ATLVGYFITLSVHLLQISVLLLVQYTNLIKAQTGFVVYLIVNLVFVVVKNIVISKRVD
                     KLYPHLSDRELARQTQELNEEEKRSIFKNVIGLACYKISNVTLSSTDSIIISSYINVA
                     AVGIYSNYVLILNIVITLVGIVFGAFTAGIGNLYVTESKEKSEFIFRCLNLLNFWVYG
                     ACGICFFYLVNPFIRLWVGEDYLFPIAVVVVIVANFLTDGLQNAVISYKDACGLFWEG
                     KFRPIASALLNVVVSLIMVRYLGIAGVILGTIISRFLTTWWFDALLVHKRAFNKSPAG
                     YYFRYLRYLAIIVATALIVYPVANMIEVTNLILWMVKGIVCFFIPNVVFLTIFRNTEE
                     FRYIKLIGKDLLTHIFKR"
     gene            4977..5291
                     /locus_tag="PLI11_02515"
     CDS             4977..5291
                     /locus_tag="PLI11_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPZ51768.1"
                     /translation="MSNHYPMNICGNLIKHARNAANMTQEQLAAKLQLDGIMLSRVQI
                     SKIEKGYRYITDQELAGFVRILGKNLLQEFWNEINKGGDGQIYRSRDKKASSVAENDK
                     FE"
     gene            5457..6092
                     /locus_tag="PLI11_02520"
     CDS             5457..6092
                     /locus_tag="PLI11_02520"
                     /inference="COORDINATES: protein motif:HMM:NF020349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HPZ51769.1"
                     /translation="MAVELDSDLVIIDDSFIEVDCRQLKNEIERAEYNGTTIVFAGSD
                     IYKCKDIGKYENVFFVDKTLNKEEVIKHINLVIQHGMTDVETKSKISIFDHAEFPIEI
                     YRQATKLLVNCGFNPKHKGFLYLRDASIMTLLEIRGGSLSKDIYPVIANRYNVSDESV
                     ERAIRHAVSKVWQTNRLEPLSKFGIKYRFDDRPTNSVIISCFTDILRELNG"
     gene            complement(6097..>6387)
                     /locus_tag="PLI11_02525"
     CDS             complement(6097..>6387)
                     /locus_tag="PLI11_02525"
                     /inference="COORDINATES: protein motif:HMM:NF020349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HPZ51770.1"
                     /translation="NFKGFHYLRHALILVAQDSEKLFSVTKILYSQIAEQFGVSNRSV
                     EHSIQTAIQSIWNSCNFENLKEALGFTINTNQSPTNVEFISLLSDAIRLNMI"
BASE COUNT         2042 a         1000 c         1443 g         1902 t
ORIGIN      
        1 tgagtgtgat ttgttttttg acaagccgtt gctagaaaag gttgtcagag gtgaaggaga
       61 ctgcagcata ctggtaagcc catataacag ggcaaccatg gatggaacag ttattcgcac
      121 agatggcgac aaggcaaagg ctcttattat caaaaagcac caaattaaag actttgatta
      181 ttcaggctac atgaaaacag taaatgtgta caagtttaag aaggacttca tgcttaatca
      241 tttcttgccg gctgtcagta cctatgtgca atatgagtca gtaaacagct attacgaact
      301 tgttctcggt tctttgatct attatggaaa cagtgatatc agggttgtta tgattgagga
      361 agatgcgtgg gcggaaattg ataatgctga agaccttgca agagcagaaa aaaagtttaa
      421 aaggtgagta gattgtacaa ggatgtaatt ggtattgtgg gaggaatggg ttcctttgcg
      481 acctgtgatc tgtttagacg acttattgag gctttccctg ctgagaagga gtgggacaga
      541 ccaagaatag ttattgacaa caactgcaca atgccaagcc gggttagagc aataatttat
      601 aaagaagaaa gaccaaagct tgtagaactt ttgacagagt cagttatcaa tcttagaaac
      661 atgggatgca ccaagataat actggcctgc aacacatccc atgtgtttct tgaggagata
      721 tacgaactgt atgagaaaaa gacaggtaat cagaactgta atattatgaa catgattgag
      781 ataattgcag aaagtcttca tgcccaaggt ataaaaaagg ttgctttgat ggcaacggaa
      841 ggaacaatgg actcgaagat atatgacgag tatttgtcaa agtacggaat tgaggtcata
      901 cctcctaaaa aggaagatta tgtagagttt cgttatctta ttgaatgtgt caagcagaac
      961 aaggtagacg aggaggcggt aaatcgtttt atcaatagtg ctgaaaatct tggttgcaga
     1021 gatgtaatac ttggctgcac agaatttcct gtatttttaa gatatatgag caggatgccg
     1081 aatgtgaatc tgcatgaccc tcttgaatat ttaataacca aattaaagaa agaactgatg
     1141 tagagagatg aagatttacg acaagctttt aaatcactat caggtttttt tcaataagag
     1201 aataaagtat cttagaagga aaagaaacat acttttatat tgtgcctccg ataccatggt
     1261 ttggcacatt ctcaattatt atgaccaggt aaaggagaac cctgtataca atttctactt
     1321 atactacggc gatgctaaaa cagataacga aacaagaaca agaagaacat acgagataac
     1381 aaagggaaca cgcatagaac caataacaaa caagaaaaga cttgtactcg ttccgctgga
     1441 tttgattgtg acagcggatc ttgattatga acccttaagg tacacaaaag atatcgtccc
     1501 tattttatat gtaaatcatg gctttcacat agtaagcata gacaatgata gaaatttata
     1561 tgcttatggg gaaagagcat tggatgataa gggaagatgc aagttttcct gtatactcga
     1621 ggcaaatatg cgcatagcca atgttatgaa caaaagagag ccttttaagg gacttgtaat
     1681 gcatgtggga gcaaaagacg gagacattat cgaacgagaa gttgataatt acagtatgta
     1741 cagaaaaaaa cttggcatag aggaagaaga gactttggtt tgtgtttttg ggacctggag
     1801 aaaggaaagt ctctttcagt atcttggcaa ggagtttctc gaagcgatgg gcagtatgac
     1861 agacaagggt tacaagtttg ttctatcaat ccaccccagg gagtatgtca aatacgacga
     1921 ggatgtcgaa cctatgggcc atgtgctgga ggagtatgcc caaaaatatg gatttattgt
     1981 taggcatcca aaggacagct atattcccta tcttattgca tcagatatag ttatatccga
     2041 ctacacatcc ctttatgaaa atgcaattct tgcaggaaag aagctttgtc tcagctattt
     2101 tccggaaact tcagtggggg atttatccat tgccaatttc ttgagaaatg aaatcaaggt
     2161 tttgagaagg gaagatataa aagagcttgg atggcacttg aatgaaataa aggttgcgga
     2221 tttttctgat attctagaaa agtacagaaa agagatttat ccgggcatag gagtttacaa
     2281 aaaccgtgtc aaagaaataa ccaggaagct ggtgggattg aaatgaaaag ggaattggta
     2341 agtgttattg ttccggtata caatactgaa aaatatctga aaaaatgtat agattcaatt
     2401 ctcaaccaga cgtacaagga aattgaactg attcttgtag atgatggctc cactgacaat
     2461 agccttgaaa tattgcgtga atacgaaagg caggacacca gggtttttgt gataccacag
     2521 gagaacaagg gggtctccgg agcgagaaac agaggacttg aggcggccag gggcaagtat
     2581 atagggtttg ttgattccga tgattacatt tctttatata tgattgaata tttggtcggt
     2641 cttcttgagg aaggtaatga ttttgcttcc tgcggttgga tttatgaaac cgaggaaggt
     2701 gaaagagagc aatctacgag tggaagtata aaagaaatgg aaactcggaa tgatatactg
     2761 aaatgcatgt ttgagcaagg ggggtgtgcg ccaaacgttt gtaacaaact attaagaggg
     2821 gatgttgtaa gggagcatgg cattcggttt ccggagggta tcaagattgg agaggacatg
     2881 attatgctgt gccagtacgt ccttaattgt gaaaaaggag tatatgatcc aaatcctttg
     2941 taccactacg tgacaagaaa agactctgca tatcacaagc ggattgtaaa agggagcttt
     3001 gatgaaagtt tcgtgaatga gattaaagcc catgaaattg ttttggaaat gctgcctgac
     3061 tgtggggcaa gaaaaagctt tataaacaaa ttgtattatg taaatttgag aaccatgatg
     3121 tttcttgctc atgcaggatt tccgattcga gaattgtatg attatcttat tcgctttcta
     3181 agaagcaatt taactggtta cctgtcatca gacagaggtt tgaagcagaa gatacatgcg
     3241 ctgttaatgt gtacaagtcc aagactatgg cattatattt tcaaggatag agggtaagta
     3301 aatggcagcg agaaaatcaa gaagccttta ttcaactaaa aatatcataa cgggtattgg
     3361 tggacagata ataaatattg tgttgggttt tgttgcccga acctttttta ttaatgcttt
     3421 gggacaggaa tacctgggtg taagcagttt gtttaccacc attcttacca tgcttaacat
     3481 ttcagagctt ggtatttcca ctgccattgt tttcagtctg tacaaaccat tggcagaaaa
     3541 ggattatgaa aaatgtcaga tacttttaaa actgctaaag aaaatatatg cggtcatcgg
     3601 aattgttgtt ctaggtatag ggctttccat aatgcccttc ctaccatata taatcaaggg
     3661 gaataccgaa gttgcaaata ttaaagtaat ctacattatt taccttttgc agtctgcttt
     3721 gtcgtactgg ttttactcct ataaatacac tattcttcag gcagaccaga ggcagtacgt
     3781 tgcaacactg gtaggttatt tcataacctt gtctgtgcat ctacttcaaa tttcggtact
     3841 gctcttggtg cagtatacaa atctgataaa agctcagacg ggttttgtag tctatcttat
     3901 tgttaatctt gtttttgtgg ttgtgaagaa tattgtcatc tcaaaaagag tggacaaact
     3961 gtacccgcac ttgtctgacc gagagctggc cagacaaaca caggaactca atgaggagga
     4021 aaaacgcagc attttcaaga atgtcattgg gcttgcctgt tacaagataa gcaatgttac
     4081 tctttcttct actgacagta tcattatctc cagctatatt aatgtggcag cggttggtat
     4141 atactcaaac tatgtattaa tccttaatat agtcattacc cttgttggta ttgtttttgg
     4201 cgcttttacc gcaggtattg gaaatctgta tgtaacagag tccaaagaaa agagtgagtt
     4261 tatttttaga tgtttaaacc tattaaattt ttgggtatat ggtgcatgcg gtatttgttt
     4321 cttttatctt gtgaatccgt ttatcaggtt gtgggtggga gaagattacc tgttccccat
     4381 agctgttgtt gttgtcattg tggcaaattt tttgaccgac ggtcttcaaa atgcggtcat
     4441 atcatataag gacgcctgcg gcttgttttg ggagggtaag ttcagaccta ttgcttccgc
     4501 cttgctgaac gttgttgtct cacttatcat ggtcaggtat ctgggtattg ctggtgtaat
     4561 tctaggtaca attattagca gattcctgac aacatggtgg tttgatgctt tgcttgtcca
     4621 taaaagagct ttcaataaat cgccagcagg ttactatttc agatacctta ggtatcttgc
     4681 aattatcgtt gcaacggctt tgatagttta tcctgttgca aacatgattg aggtaaccaa
     4741 tctgatttta tggatggtaa aaggaatagt atgttttttt atccctaacg tggttttcct
     4801 gacaatcttt agaaacaccg aagaatttcg atatattaag ttgatcggga aggatttgct
     4861 cacacatatt tttaagcgtt aactgcctca tgttccctgt gggaacaatt ttgcccttta
     4921 taaatgttaa aatcatgtag tttataatat ttagctggac acaagggggc aaaagtgtga
     4981 gcaatcatta tccaatgaac atctgtggca atttaataaa acatgcccgg aatgctgcga
     5041 atatgacgca ggaacaattg gcagcaaagc ttcaacttga tggtataatg ctctcgcgtg
     5101 ttcaaataag caagatcgag aaaggataca ggtacataac ggatcaagaa cttgctggtt
     5161 tcgtaagaat tttgggtaag aaccttttac aggagttttg gaacgagatt aacaagggag
     5221 gagacgggca gatttaccgc agcagggata agaaagcttc aagcgttgct gagaatgaca
     5281 agtttgagta atcatggagg tttttatcaa tgcaaaattc tggtgttacc aaaccgctaa
     5341 atattataat cgcgctacca gaatcagatt tttttaaaga gtttgtatca tatataatct
     5401 cgcatcatac tattaaaaac ctaggtttcg cagaaaaact caaagaagct ttccccatgg
     5461 cagtagaact agattctgat ttagtaataa tcgacgactc gtttattgag gtggattgca
     5521 ggcaacttaa aaatgaaatt gaaagagcag aatacaatgg aacaaccatt gtttttgcag
     5581 gcagtgacat atataaatgc aaggatattg ggaaatatga gaatgttttt ttcgtagaca
     5641 agacgttaaa caaagaagag gtaataaagc acatcaattt ggttatacag catggcatga
     5701 cggatgtgga gacaaaatca aagatctcca tatttgacca tgcggagttt ccaattgaaa
     5761 tatacaggca ggcaacaaaa cttcttgtca attgtggctt taatccgaag cacaagggtt
     5821 ttttatactt gcgtgacgca tctattatga cactgcttga aataagggga ggaagtcttt
     5881 ccaaggatat ttatcctgtt attgcaaaca ggtacaatgt aagcgatgaa agtgtggaaa
     5941 gggcaattag gcatgctgtg tccaaggttt ggcaaacgaa ccgcctggaa cccttgtcca
     6001 agtttggaat taagtatcgg tttgatgaca gaccaacaaa ttcagttatt atatcctgtt
     6061 ttacagatat tctacgggaa ctcaacggat aattacttaa atcatattca gtcttattgc
     6121 gtcggataaa agtgaaatga attccacatt tgtgggggat tgattggtgt ttattgtgaa
     6181 gcctagagct tcttttagat tttcgaaatt acatgaattc catatggatt gtatggcggt
     6241 ttgtattgaa tgttcaacac tgcggttgga tactccgaat tgttcggcta tttgagaata
     6301 aagtattttt gtaacggaga acagcttttc gctatcttgt gcaacaagta taagtgcatg
     6361 acgtagataa tgaaaacctt tgaaatt
//
LOCUS       DAONVY010000056         6384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9122_length_6384_cov_3.261337, whole genome shotgun sequence.
ACCESSION   DAONVY010000056 DAONVY010000000
VERSION     DAONVY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9122_length_6384_cov_3.261337"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1576
                     /gene="rny"
                     /locus_tag="PLI11_02530"
     CDS             8..1576
                     /gene="rny"
                     /locus_tag="PLI11_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPZ51771.1"
                     /translation="MLFIVVPLVFSSLVGFICWKIASKRSFAKGYQARKEEAEAVIES
                     AEAEGKRIISEAIKESEIKKREYLLTAKQEIHKSRIDLEREIKDRRNEIQRNERRLIQ
                     KEELLEKKQDAQEQKERMLNERMLELDEREKHVEEMFNKQVQELERIANLTKDEAKEY
                     LLRLTENELIQEKVTLIKDYEAKLKEECVVLAQNLVATAIQKCASDYVSETTVSVVAL
                     PNDDMKGRIIGREGRNIRTIETLTGVDLIIDDTPEAVILSSFDPIRREIARIALEKLI
                     IDGRIHPARIEEMVEKARKEVDNVIRQEGENATYETGVYGLHPEIVRLLGRLKFRTSY
                     GQSVLLHSIEVSKLAGVMAAELGVDVALAKRAGLIHDIGKALDSEIEGSHVTIGADIA
                     KKCKESPDVVNAIISHHGDIEPQSVVAVLVQAADTISAARPGARRETLETYIKRLEKL
                     EEIANSFSGVEKTYAIQAGREIRIMIKPELVSDEEMPLKAREIVKKIESEMEYPGQIK
                     VTILRETRAVEYAK"
     gene            complement(1642..1923)
                     /locus_tag="PLI11_02535"
     CDS             complement(1642..1923)
                     /locus_tag="PLI11_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HPZ51772.1"
                     /translation="MDVLKVSARSNPASVAGALAGVIREVGSVDVQAIGAGAINQAIK
                     AIAITRGYLAPTGIELVCMPAFVDVYLEGEERTGIKLHVERRNYRGYCG"
     gene            complement(2115..2891)
                     /locus_tag="PLI11_02540"
     CDS             complement(2115..2891)
                     /locus_tag="PLI11_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPZ51773.1"
                     /translation="MKILFIGDVFARPGRQALNLWLKDLISEHDIDFCIANGENAAHG
                     KGITKQTADEIYASGVDFITLGNHAWGQKDTLSFIDDFPIVRPANMSKSLPGKGWCIV
                     NTNKGDIGILNLQGRVFMDHVDNPFECATECLHEMRQYTNIIVVDFHAEATSEKIALA
                     LYIDGLCSCVIGTHTHVQTADERILPNGTAFISDAGMTGPIHSIIGMDIKCVMEKFVR
                     SMPTRFEPAKGSFMLNAVVIDICDNSGVAKDIYRIFLKQS"
     gene            2988..3719
                     /gene="pgeF"
                     /locus_tag="PLI11_02545"
     CDS             2988..3719
                     /gene="pgeF"
                     /locus_tag="PLI11_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPZ51774.1"
                     /translation="MRQVCINQEKNDIIQLFTTRYTGNMSHTREENKQNVDKRFEMIA
                     NTFNMSVDRMVLLNQTHGTDIFVADESYIRGEKPVCDASVTNVPGICLVTIHADCVPV
                     YFHDKKTKSIGLAHSGWKGTLDGISIHVLDKMNEVYGTKAEDVEIVIGPCICKSCFEV
                     KNDVYSLFAGKYPDYADCITEGNIDLKGVIIRGLLDKGVREENIIDMDICTCHNEDLF
                     FSHRRGTKRNKSGEGAMAALACLKG"
     gene            3723..5324
                     /locus_tag="PLI11_02550"
     CDS             3723..5324
                     /locus_tag="PLI11_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ51775.1"
                     /translation="MKRVLVSVVVMVMLFLAGCGDSNDVNTTPQPTMNINEPMPTPVG
                     ELKVKPVSGGTVTLGVSKFRTLNPLVADNEDMKHYLNLVYDPLFKLDFQQNVLPCLAV
                     SAKTQDKGMSWEISLRKDVVWHDDKAFSAADCIHTINFLMKNDSLYSANVANIEECTL
                     IDEYTFSIKLRKPDMYVAGKLTFPIVSATATGIDKNAVGTSMYRHIITEDNKMVLVAN
                     DKYWGKMPYIETVEIHFFDNERLKYESNCDLLLLTGSNVTRYMNKAGYISYKYTGRNY
                     VCLVPNLRTNNVAMDVNIRKALMHIIDFERVINASVSGNGIKAAWPVMPGTRYTKESS
                     HMYERDLNKAVEYLERAGYRKLDDGYWYKASDTQRRYPLTIPVNVLSVDTEMRLCAEN
                     IKKQVEEIGIIFSINYQTEADFETALTSNNFSCALLSFYIGSWPDIEPLFATEGVMNL
                     SGYTNEELDGLLAGLFEKDSILDDMTRIKGILDDDLPIMGLYISQEAIVISEKIYGKD
                     AEYNFWSLLDNFGNLHMFTEGIIEE"
     gene            5342..5872
                     /gene="lspA"
                     /locus_tag="PLI11_02555"
     CDS             5342..5872
                     /gene="lspA"
                     /locus_tag="PLI11_02555"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPZ51776.1"
                     /translation="MFFVVSILLIIADQIFKIIVSSRIPYGSGIEVIPGFFELVYWKN
                     TGAAWGIFSDGTFYLTIISAVASLVIIALIPKARSLFLKTSLSLILAGAVGNLIDRVR
                     LGYVVDFLSFDIFGYDFPVFNLADMCIVIGCILMIIYVLFIHKENQPLFNNFVFIQKL
                     KVRSKKSSEEEDAGVQ"
     gene            5886..>6384
                     /locus_tag="PLI11_02560"
     CDS             5886..>6384
                     /locus_tag="PLI11_02560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPZ51777.1"
                     /translation="MEYIEAVVSAEYQGERLDVFLTEYLADYFDAISRSYVQKLIKDG
                     LVTRKGKVVKANEKVKENDLFDVQFKEPAELEAKPENIPVEIVYEDEDIVVVNKERGM
                     VVHPAAGNHSGTLVNALLYHCKDLSDIGGTIRPGIVHRLDKDTTGLMVVAKNNMSHLF
                     LSSEIK"
BASE COUNT         2032 a         1064 c         1469 g         1819 t
ORIGIN      
        1 cgatattatt ctattcatag tcgtcccttt agttttcagt tcactagttg gctttatttg
       61 ttggaaaata gcctcgaaaa gatcttttgc taagggttat caagccagaa aagaagaagc
      121 ggaagcggtt atagaaagcg cggaagcgga aggcaaaaga attattagtg aagcaattaa
      181 agaaagtgaa ataaagaagc gtgagtattt gctcactgcc aagcaagaga ttcacaaaag
      241 tagaattgat cttgaaaggg aaattaaaga tagacgaaac gagatacaga gaaatgaaag
      301 acgtttgatt cagaaagaag agttgcttga gaaaaagcag gatgcgcagg aacaaaagga
      361 gcgtatgctc aatgaaagaa tgcttgaact tgacgaacgt gaaaaacacg tagaggaaat
      421 gttcaacaaa caagtccaag aactcgaacg tatcgcaaat ttgacgaaag atgaagcaaa
      481 ggaatatttg ctaagactta ctgaaaacga attgattcag gaaaaagtaa cacttatcaa
      541 ggattacgaa gcgaaattga aagaggaatg tgttgttttg gcacagaact tagtagcaac
      601 ggctatacag aaatgtgcat ctgactatgt ttcagaaacg actgtatctg ttgtcgcatt
      661 acctaatgat gacatgaaag gcagaatcat aggtagggag ggcagaaaca taaggaccat
      721 tgaaacattg actggagttg atctgatcat agatgacaca cctgaagctg ttattctatc
      781 aagttttgat ccaattcgac gagaaatagc taggatagct ctggagaagc tgatcattga
      841 cggtagaata catcctgccc gaatcgaaga aatggtagag aaggcgagaa aggaagtaga
      901 caatgtcatc cgtcaggaag gagagaacgc tacatacgaa actggtgtgt atgggcttca
      961 tcctgaaatt gtaaggcttc tgggcagact gaagttcagg accagctatg gacaaagcgt
     1021 tctcttgcac tcaattgaag tttctaaact ggcaggtgtc atggctgcag agctgggtgt
     1081 agacgtagct ttggcaaaaa gagccggctt gatacacgac ataggcaagg ctctggacag
     1141 cgagattgaa ggctcgcatg taaccatagg tgcagacata gccaagaagt gtaaagagtc
     1201 gcctgatgtt gtaaatgcca taatatcaca tcatggagat attgaaccgc aaagcgtagt
     1261 tgctgtacta gtacaagctg cagacacaat atcagcagca aggcctggtg caagacgcga
     1321 aactctcgag acttatatca agagactaga gaaactcgag gaaatagcga attccttcag
     1381 tggcgttgaa aaaacttatg cgattcaggc tgggcgagaa atcagaatta tgattaagcc
     1441 ggaacttgtc agtgatgagg aaatgccttt gaaggcaaga gagatagtta agaagattga
     1501 aagtgaaatg gaatatcctg gacaaatcaa agtaacgatt ttacgggaaa caagagcagt
     1561 agaatacgca aaatagtttg aaaacaaaaa agagagtgat tgattcactc tcttttttaa
     1621 gtatgtttct aattttttgg gttatccgca ataaccccta taatttcttc tttcaacatg
     1681 aagtttgatg cccgtacgtt cctcgccttc aagataaacg tcaacgaaag cgggcatgca
     1741 taccaattcg attccagtcg gagccaaata ccctctggta attgctatag cctttattgc
     1801 ctggttaatt gcacctgcgc cgattgcctg cacatcgaca gaacctactt ccctgataac
     1861 acctgccaat gcacctgcta ctgaagctgg atttgatctt gctgatacct ttaatacatc
     1921 cataatatgt accctccatt atttttttat ttttttgatt gtttttatat attctaaata
     1981 aaaacatgta gcaatacaat tatttcccaa aatagtgggg tgtttgtggt acatatatat
     2041 gcatcatatc gttatttatg aataaataat ggagggaaaa ttctaaagtt aggcatgttg
     2101 aatatttccc gactttagct ttgtttcaaa aaaatcctgt agatatcttt ggcaactcct
     2161 gaattgtcac aaatatctat tacaacagcg ttcagcataa agctgccttt tgcaggttcg
     2221 aatcttgtag gcatggagcg aacaaacttc tccattacac attttatgtc catgccaatt
     2281 atactgtgta ttggtccggt cattccggca tcggaaatga aggcagtgcc gtttgggaga
     2341 atgcgttcat ccgctgtctg gacatgagtg tgggtgccaa ttacgcagga acacaatccg
     2401 tctatataca gcgccaatgc gattttttca gacgtggcct ccgcatgaaa atccacaaca
     2461 atgatatttg tatactggcg catttcatga agacattcag tggcacattc aaagggattg
     2521 tccacatggt ccatgaagac acgcccttga agattcaaaa tgcctatgtc tcccttgttt
     2581 gtgttgacaa tacaccagcc tttgccggga agagattttg acatgtttgc tggtcttaca
     2641 attgggaagt catcgatgaa cgaaagagta tctttctgtc cccaagcatg gtttccaagt
     2701 gtgatgaaat ccacaccact agcgtatatt tcatctgcag tttgctttgt gatacctttt
     2761 ccgtgtgccg cattttcgcc gttggcaatg caaaagtcaa tatcatgctc gcttattaga
     2821 tctttaagcc agagatttag tgcctgacgt cccggacgtg cgaatacgtc cccaataaaa
     2881 agtattttca cagcgtttac ctttctaatt acatgtatta tttgaataaa tattggattt
     2941 gatatataat attatcactt taaaaggatg gggtcaagat tttgcctatg agacaagttt
     3001 gtataaatca ggagaaaaac gatataattc agttatttac cacaagatac acaggtaata
     3061 tgagccatac aagggaagaa aacaaacaga atgttgataa aagatttgaa atgatagcca
     3121 atacattcaa catgagtgtt gacagaatgg ttttacttaa tcagacacat gggacagata
     3181 tttttgttgc tgacgaaagc tacataagag gagaaaaacc tgtttgcgat gcatctgtga
     3241 caaatgtgcc tggcatttgt cttgttacca ttcatgctga ctgtgttcct gtatatttcc
     3301 atgataaaaa aacgaaaagc atcgggcttg cccattcggg ttggaaggga acattggacg
     3361 gcataagtat acatgttctt gataaaatga atgaggtata cggtacaaag gctgaggatg
     3421 tggaaattgt cataggtccg tgcatttgca aaagttgttt tgaagttaaa aatgatgtat
     3481 actctctctt tgctggcaaa tatccagatt atgctgattg cattacagag ggaaatattg
     3541 accttaaagg agtaattata cgtggtctgt tggataaggg agtcagagag gagaatataa
     3601 ttgatatgga catatgtaca tgtcataatg aagatttgtt cttttctcac cgaagaggca
     3661 ctaaaagaaa caaatctggc gaaggcgcaa tggctgcgct tgcctgtttg aaaggataga
     3721 ttatgaaaag ggttttagtc tcagttgtcg ttatggttat gcttttttta gcgggctgcg
     3781 gagattcaaa tgatgttaat acaacgcccc aaccaacgat gaatattaat gaaccgatgc
     3841 cgactcctgt aggagaatta aaggtaaaac ctgtcagcgg tggcactgtg acattgggag
     3901 tttcgaagtt tcgaaccctt aatcctctgg tagctgacaa tgaggatatg aagcactatt
     3961 taaatcttgt ttacgatcct cttttcaaac ttgactttca gcagaatgtt cttccttgtc
     4021 ttgctgtcag tgcaaagaca caggacaagg gcatgtcatg ggagataagt ttaaggaagg
     4081 atgttgtatg gcatgatgac aaggcttttt cagctgctga ctgcattcat acaattaatt
     4141 ttcttatgaa aaacgacagc ctttacagtg caaatgtggc taatattgaa gaatgcacgc
     4201 ttatagatga atatactttc agcataaagc tcaggaaacc tgatatgtat gttgcgggaa
     4261 aacttacatt cccgattgta tccgcaactg caacaggaat tgataagaat gctgtaggca
     4321 cttccatgta cagacatatt attacggaag ataacaagat ggtgcttgtg gcaaatgaca
     4381 agtattgggg taaaatgcca tatattgaaa ctgtggaaat ccactttttt gacaatgaaa
     4441 ggcttaaata tgagtcaaac tgtgatttgt tgttgttgac aggttccaat gtcacaaggt
     4501 atatgaacaa ggcaggatac atatcctaca aatatactgg aaggaattat gtatgtcttg
     4561 ttccaaattt aagaaccaac aatgtggcaa tggatgtgaa tattagaaaa gctcttatgc
     4621 acattattga ctttgagagg gtaattaatg caagcgtgtc gggtaacggc ataaaggctg
     4681 catggccggt catgcctggc acaagatata caaaggaatc ttcccacatg tatgagaggg
     4741 atttgaacaa agctgtggaa taccttgaaa gggccggtta tcgaaagctg gatgacgggt
     4801 actggtataa agcgagcgat actcaaagaa gatatccact gacgattcct gtcaatgttt
     4861 tgtctgttga cacggagatg cgtctatgtg cggaaaacat aaaaaaacag gtcgaggaaa
     4921 ttggtataat attttcaatt aactatcaga ccgaggcgga ctttgagaca gctttaacga
     4981 gcaacaattt ttcatgtgcg ctcttaagtt tctatatcgg atcctggcca gatattgaac
     5041 ccttgtttgc aacggaggga gtcatgaatc ttagtggata tacgaatgaa gaacttgacg
     5101 gtcttttggc aggtttgttt gaaaaggact ccatcctgga tgatatgacc aggataaagg
     5161 gtatattgga tgatgatctt cctataatgg gtctttatat atcccaggaa gcaatagtga
     5221 tttcagaaaa gatatacggc aaggatgcag agtacaactt ctggagtctg ttggacaatt
     5281 ttggaaacct gcatatgttt actgaaggga ttatagagga atgaataagt ggaggaatga
     5341 gatgtttttt gtagttagca ttctgctgat aatagcggat cagattttca aaataatagt
     5401 ttcatcaagg ataccatatg gtagcggtat tgaagtgatt ccaggttttt ttgaactggt
     5461 ttattggaaa aacacagggg ctgcctgggg aatattttca gatggaactt tctatcttac
     5521 gataatatcc gctgttgcgt ctcttgtaat tattgctttg atacctaagg ctagatcgtt
     5581 gtttttaaaa acatcattgt ctttgattct tgctggagca gtagggaatc tgattgaccg
     5641 tgtaagactt ggctatgtgg tagatttctt gtcatttgac atattcggat acgattttcc
     5701 agtattcaat ttagcagata tgtgcatcgt aattggatgt attttgatga ttatctatgt
     5761 attattcata cataaggaaa atcagccttt gtttaacaat tttgtgttta ttcagaagtt
     5821 gaaggtaaga agcaaaaaat catcagaaga ggaagatgcc ggtgtacaat gatgacatgg
     5881 aggatatgga atatattgaa gcggtagtaa gtgctgaata tcagggcgaa agactggatg
     5941 tttttctaac cgagtatttg gcagactatt ttgatgccat cagtagaagc tatgtacaga
     6001 aactcataaa agatgggctt gtaacaagaa agggaaaagt tgtcaaggca aacgaaaagg
     6061 tcaaggaaaa tgaccttttc gatgttcaat tcaaggagcc tgcggagctg gaggcaaaac
     6121 ctgagaacat acccgttgaa atagtttatg aagatgaaga tattgtggtg gttaacaagg
     6181 agaggggaat ggtggtacat cctgcggctg gcaaccattc aggaaccttg gttaatgctc
     6241 ttttgtatca ctgcaaggat ctgtcggata ttggaggtac cataagaccg ggtattgtgc
     6301 acaggcttga caaggatacc actggtctca tggtggtggc caagaacaat atgtcccatt
     6361 tgtttttgtc gagtgaaata aagg
//
LOCUS       DAONVY010000057         6374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9139_length_6374_cov_3.690141, whole genome shotgun sequence.
ACCESSION   DAONVY010000057 DAONVY010000000
VERSION     DAONVY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9139_length_6374_cov_3.690141"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLI11_02565"
     CDS             complement(<1..965)
                     /locus_tag="PLI11_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51778.1"
                     /translation="MMTREVALEIIDYILGVSVINVTTKISELVEVINKQNNYKVTEN
                     DILAVYDMYERDLGVVIVEDMIVNYMFLIDEKFTEEVIEYLADVDLHKASKEEIFMHG
                     KMDSGPAGNSFRLIEFFVKYFKEHGINEAETGINEKHIQLFEKLINYYYTGEVDEDML
                     NEFADYVAGISDFEDYDEFDEYVDEDDDFDFDLDDEFEPGMTLLRNNSYVVSVSLGTG
                     CYRHIQINGNSTLEELHSAILDAFEFDDDHAHAFFMTNREWDSQGAFYADSLEDERRH
                     TSDYILNHFEFEKGDKFKYLFDFGASWVFQCKVLREENKVVDEPKV"
     gene            complement(1059..1739)
                     /locus_tag="PLI11_02570"
     CDS             complement(1059..1739)
                     /locus_tag="PLI11_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HPZ51779.1"
                     /translation="MQKQDLKQYLVKEVTSQELIELVSENIKPFYELMAYYRCAMMEV
                     ETKFRVLDEHFSLKYNSNPIESIKSRLKAPESIAEKLSRRGFPLTIESIEKNIYDVAG
                     VRVICSFPRDIYMLADLLLMQDDVVLVEKRDYIKNPKPSGYRSLHLIVEVPIYLHSGK
                     KMVKVEVQLRTIAMDFWASIEHKLRYKNNINKKEVKTIAEELLECSRVSAMLDKRMEQ
                     IKEKIDKL"
     gene            complement(1818..2639)
                     /locus_tag="PLI11_02575"
     CDS             complement(1818..2639)
                     /locus_tag="PLI11_02575"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPZ51780.1"
                     /translation="MFIEILKAIILGLVEGITEWLPISSTGHMILLEEFIKLKTSEEF
                     KEMFFVVVQLGAILAVVFLYFNKLNPLAPSKSVEEKKATMSIWYKVVIGVIPAGVVGF
                     LFDDWLNERLYNYITVAITLILYGILFIVIENRNKNRKTRINSISELDYLTVFLIGVF
                     QILSLIPGTSRSGVTILGAILLGASRQIAAEFSFFLSIPIMFGASFLKLVKFGFSFTK
                     LEAVILLTGMVVAFAVSIIAIKFLLGYVKRNDFKAFGYYRIALGVLVVGYFLLVG"
     gene            2756..3217
                     /locus_tag="PLI11_02580"
     CDS             2756..3217
                     /locus_tag="PLI11_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51781.1"
                     /translation="MENYEDIMRKGNISRNMDNYPQTLKEINPENMGKTMAYRLIYPE
                     IYYKLQPYIMMVCDQMDVYNTVPTQEMVESMTDAIYDDVVRMFPDIAEYVREHEDSEV
                     RETIQPDAITVQVDPARFRDRTRFPRFRRRGLFKDLIDILLLTELLRRRRR"
     gene            3330..4247
                     /gene="trxB"
                     /locus_tag="PLI11_02585"
     CDS             3330..4247
                     /gene="trxB"
                     /locus_tag="PLI11_02585"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPZ51782.1"
                     /translation="MYDVIIIGGGPAGYTAALYSARARLKTLVIEKMFSGGQMATTDS
                     MENYPGFDEPVGGVELALRMENQARKFGAEILYDEVIDMDLAGKVKIVKTTSHTYNCK
                     ALILCMGASPKSLGLDKEGALRGLGVSYCSVCDAAFYKGKKVAVVGGGNSAADDALYL
                     TKYAEKVYLIHRRDNLRCVKMAQEELFKSDKIDFVFNSQVVELLGERKLEGIRVKNNS
                     TNEASTLDIDGLFIAIGLVPNNELVKGKLELSKDGYIITDRNMKTSVEGVFAAGDICE
                     KPLRQVVTAASDGAVAAYSAEKYVTQYNP"
     gene            complement(4228..5187)
                     /locus_tag="PLI11_02590"
     CDS             complement(4228..5187)
                     /locus_tag="PLI11_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPZ51783.1"
                     /translation="MKYIDLHCDTLSKAYSHNVDDIGNWPLAMVDIDRLYKADCIAQF
                     FAIYMPDERNEDFNDDEYILMLVELLKCNIKRYEDKIAYARNYGMLVENDRKQKISAF
                     LTIEDGRSVNGCFEKIKKYYDLGVRLITLTWNSPNCFGYPNSFDKNIMEKGLTSFGMD
                     AVSYMNDLGILVDVSHLSDGGFYDVCEVSKKPFVASHSNCRELSPHPRNLTDDMIRLL
                     ASKGGVIGVNIVPGFLSSDIGSRESFIDDVARHVVHMISKGGIECVCIGTDFDGTGGS
                     IELNEPAKVQLLFERLKKMGIREDALGKIAYRNATRVIKDCIG"
     gene            complement(5188..5949)
                     /locus_tag="PLI11_02595"
     CDS             complement(5188..5949)
                     /locus_tag="PLI11_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HPZ51784.1"
                     /translation="MRFDDFDKMMRKYEESLDQVIVPDVHIVARLDGRGFTRLTKEDM
                     KFEAPFDVLFREHMVETVRHLMNCGFNIVYGYTQSDEISLLFHRDDNTFGRNTRKINS
                     ILAGEASAWMSLALSKLAVFDCRVVPLPNLDRVIDYFLWRQEDAHRNSLNAHCYWILR
                     NEGMSRQEATFALCGKSTSCKNELLFKRGINFNNLPLWQKRGVGVYYKDIYVDGFNPV
                     TNSYARTKRRRLFVDYELPVKEEYGFFLEKLIEVK"
     gene            complement(5939..6373)
                     /locus_tag="PLI11_02600"
     CDS             complement(5939..6373)
                     /locus_tag="PLI11_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ51785.1"
                     /translation="MIFTGLQASGKTTFFKENFADEYVHVNLDTLRTRNKEKLLLEDC
                     FENNLSFVVDNTNPTRENRQRYISLAKEKGYKVIGYYFKSDINGCLKRNAKRNEKNIP
                     DKALFATHKKLEIPSFEEGFDELYYVHITDTGFKVEDWTDEV"
BASE COUNT         1939 a         1428 c         1101 g         1906 t
ORIGIN      
        1 accttgggct catccaccac tttgttctct tccctcaaaa ccttgcactg gaaaacccag
       61 ctggcgccaa agtcaaacaa atacttgaac ttgtcgcctt tttcaaattc aaaatggttg
      121 agtatgtagt cagatgtgtg ccttctctca tcttccaaag agtctgcata gaatgcccct
      181 tgtgaatccc actccctgtt tgtcatgaaa aaggcatggg catggtcatc atcaaattca
      241 aaagcatcca gaatggcact atgaagttct tccaatgtgc tgtttccgtt tatctgaata
      301 tgtctgtagc atcctgtacc caacgataca ctaacaacat aggaattgtt cctcaaaaga
      361 gtcataccgg gttcaaactc atcatccaga tcaaaatcga aatcgtcatc ttcatcaacg
      421 tattcatcaa actcgtcata atcttcaaaa tcactaattc cggcgacata atctgcaaat
      481 tcattcagca tatcttcatc aacttcgcct gtgtaataat agttgattaa tttctcaaac
      541 aactggatat gtttttcatt aatgcctgtt tcagcttcat taataccgtg ttctttaaag
      601 tattttacaa aaaactcaat caatctgaaa ctgttgcctg caggaccgga atccatcttt
      661 ccatgcatga agatttcttc cttgctggcc ttgtgcaaat ccacatctgc aaggtattca
      721 ataacctctt ctgtaaattt ttcatctata aggaacatgt agttgacaat catgtcttca
      781 actatcacca cacccaaatc tctttcatac atgtcataaa ctgccagaat atcattttca
      841 gtaaccttgt aattattctg tttgtttatt acttcaacca gttcactgat tttagtagta
      901 acattaatta cactaacacc tagtatataa tctataattt ccagtgctac ctctcttgtc
      961 atcataaagt ccccttattc ttttcccttc actatatatt tttaaaaaac taaagtcaag
     1021 accaaaaaaa cacaaaaatg cttgccttga atgcaaagtt aaagcttgtc tattttctct
     1081 tttatctgct ccatcctctt atccaacatt gcactaactc tggaacactc caaaagctcc
     1141 tctgcaattg tctttacttc cttcttgttt atattattct tgtaccttag cttgtgctca
     1201 atacttgccc aaaagtccat ggcaatggtt ctcaactgaa cttccacctt caccattttc
     1261 tttccactgt gcagatatat tggtacttcc actatcaaat gcaaactcct gtagccgctt
     1321 ggctttggat tttttatgta gtctcttttc tcaaccagaa ccacgtcatc ctgcatgagc
     1381 aacaaatccg ccagcatgta gatatccctt gggaaggagc agataactct gactcctgcc
     1441 acatcatata tattcttctc gatggattca atggtcagag gaaaacctct tctgctgagt
     1501 ttttcggcta tgctttccgg tgccttaagg cggcttttta tggattcaat aggatttgaa
     1561 ttgtatttca atgagaaatg ttcgtcaaga accctgaatt tggtttcaac ctccatcatg
     1621 gcacacctgt agtatgccat cagctcgtaa aagggcttta tgttttcact caccaactca
     1681 attaactcct ggctcgttac ttccttaacc aaatactgtt tgagatcctg tttttgcatt
     1741 tttccaatcc ttcaagttta cttgtttcgc taataataat atataccaaa aacgaggatt
     1801 tgtccataaa aaatgaatca gccgaccaaa aggaaatatc cgacaaccaa aacacccaaa
     1861 gctatgcggt aatatccaaa agccttgaaa tcgttccttt taacgtatcc aagcaaaaac
     1921 tttatcgcta ttattgaaac agcaaatgca acaaccatgc ccgttagaag aattactgct
     1981 tccagtttgg taaaactgaa accgaacttt acaagtttaa gaaaacttgc tccaaacata
     2041 ataggtatgg acaggaaaaa ggaaaactcc gcagcaatct gtcttgatgc tcccagtagt
     2101 atggcgccaa gaatggttac accggacctc gaagtgcctg gaattaggga gagaatctga
     2161 aaaacaccaa tcaaaaagac agtcaggtaa tcaagttcgg aaatggaatt aattcttgtt
     2221 tttctgtttt tgtttctgtt ttcaataact atgaaaagaa tgccgtacag tattagggtt
     2281 atggcaactg taatatagtt gtataggcgc tcattaagcc agtcgtcaaa caaaaaaccc
     2341 acaactccag ctggaattac accaattaca accttgtacc agatggacat ggttgctttc
     2401 ttttcctcaa cacttttact cggagccaaa ggattaagct tgttaaagta caaaaaaacc
     2461 acagcaagaa ttgcacccag ctgtacaaca acaaagaaca tctctttaaa ctcttccgat
     2521 gtcttaagct ttataaactc ctccagcagg atcatatgtc ctgtactact tattgggagc
     2581 cactctgtaa ttccctcaac caaacccagg attattgcct ttagtatttc aataaacata
     2641 aatcagaacc cccgtccata tgtatgttta atctaagaat tacattttat cacacaatgt
     2701 ctagctttac cataaaatat atcgaagcat caaaaacaaa gaatcgaggt ttggtatgga
     2761 aaactacgaa gatatcatga gaaaaggtaa cattagcaga aacatggaca attaccctca
     2821 gacattaaaa gaaatcaacc ctgaaaacat gggcaagaca atggcataca ggctgattta
     2881 tcctgaaatc tactacaaac tccagcctta cataatgatg gtatgcgacc aaatggacgt
     2941 atacaacaca gtgcccacac aggaaatggt tgagagcatg actgatgcaa tttatgacga
     3001 tgtggttcgc atgttccccg atattgccga atacgtcagg gaacatgaag acagcgaggt
     3061 acgagaaaca atacagccag acgctattac tgttcaagtg gatcctgcaa gattcagaga
     3121 cagaacaagg tttccaagat tcaggcgaag aggccttttc aaagacttga ttgacattct
     3181 attgctgaca gaactgctcc gtagaagaag aaggtaagaa agtgaagaga ccaatttggg
     3241 gtctcttcaa tattgttttc acaatatatt cactgtcatt tgacactttt tgagtataat
     3301 atatgtatca agaaaaaaga gggataaata tgtatgatgt gataattatt ggtggaggtc
     3361 ctgcaggcta tacggcagca ttgtacagtg caagagccag gctcaagact ctggtgattg
     3421 aaaagatgtt ttcaggagga caaatggcta ccacggattc aatggaaaac tatcctggtt
     3481 ttgatgaacc tgtgggcggt gttgaacttg cactcagaat ggaaaaccag gccaggaaat
     3541 ttggtgcgga aatcctctat gatgaggtaa ttgatatgga tttggctgga aaggtaaaga
     3601 tagttaaaac aacatcgcat acatacaatt gcaaggcatt gatcctctgc atgggtgcaa
     3661 gtccgaagag tcttggtctt gacaaggaag gtgcattacg cggtcttgga gtctcatact
     3721 gttcagtctg tgatgcagcc ttttacaaag gaaaaaaagt tgcagttgta ggcggaggaa
     3781 attctgcagc ggatgatgct ctgtatctaa ccaaatatgc agagaaagta tacctgattc
     3841 acagaaggga taatttaaga tgtgtgaaaa tggcgcagga agaactattc aagagtgaca
     3901 aaattgattt tgtttttaac tcccaggttg ttgaactttt gggtgaaaga aaactggaag
     3961 gaataagggt gaagaataat tcaacaaacg aagcatccac ccttgacata gatggactgt
     4021 ttattgcaat aggtctagtt ccaaacaatg aacttgtcaa gggcaaactt gaactaagca
     4081 aggatggata tatcataact gacagaaaca tgaaaacgag tgtcgaagga gtgtttgcgg
     4141 caggagacat atgcgaaaaa cccctccgcc aggttgtaac ggcggcatca gacggagcag
     4201 ttgcggcata ctctgcagaa aagtatgtta cccaatacaa tccttgataa cccttgtagc
     4261 attcctatac gcaatctttc caagggcgtc ttctcttatg cccatctttt tcaacctttc
     4321 aaacagcaat tgtacttttg caggctcatt cagttcaata ctgccgccag tgccgtcaaa
     4381 gtccgtaccg atacaaacgc actcaatacc gcctttgctt atcatgtgca caacatgcct
     4441 tgccacatca tctataaagc tttcccggct cccaatatca ctgctcaaaa aaccaggaac
     4501 aatattcact cctatcactc cgccttttga agcaagcaac cttatcatgt catcggtaag
     4561 atttctagga tgagggctta gctccctaca gtttgagtgg gatgccacaa agggcttttt
     4621 tgatacttcg caaacatcat aaaagcctcc atcggaaaga tgggaaacat ccaccaatat
     4681 gccaaggtca ttcatgtatg aaactgcatc catgccaaag ctggtcaggc ctttttccat
     4741 gatgttttta tcaaaggaat ttggataacc aaagcagttg ggactgttcc aggtaagagt
     4801 gatgagcctt actcccaaat cataatattt ctttattttc tcaaaacatc cgtttacact
     4861 tcttccgtct tcgatagtaa gaaaggcaga tatcttttgc tttctatcgt tctcaaccaa
     4921 cattccataa ttgcgggcat atgcaatttt gtcctcatac ctctttatat tgcacttcag
     4981 cagctccaca agcattaaaa tatactcatc atcattaaaa tcctcatttc tctcatcagg
     5041 catgtatatt gcaaaaaact gggctatgca gtcagctttg taaagcctgt cgatatccac
     5101 cattgcaagc ggccaattac ctatgtcatc cacattatga ctgtatgcct ttgataaagt
     5161 atcacagtgc aggtcaatat atttcattca tttcacctct ataagttttt ccagaaaaaa
     5221 tccatactct tcttttacag gcagctcata gtccacaaaa agccttctcc ttttggttct
     5281 tgcataacta tttgtcacag gattaaatcc gtccacatat atatctttat aatatactcc
     5341 aacccctctt ttctgccaaa gaggaaggtt gttaaaatta atccctctct tgaacaacaa
     5401 ttcgttcttg caagatgtgg actttccaca aagagcaaaa gttgcttcct gcctgctcat
     5461 accctcgttt ctcaaaatcc agtagcaatg cgcattcaat gagttcctat gggcatcttc
     5521 ctgcctccag agaaaatagt ctattaccct gtccagattt ggaagaggca ccaccctgca
     5581 gtcaaataca gctaatttgc ttaaggcaag agacatccag gcactggctt cccctgccag
     5641 aatggaattt atctttcttg tatttcttcc gaaagtattg tcatcccggt ggaatagcag
     5701 ggatatctca tcactttgtg tataaccata cacaatgtta aatccgcaat tcatcaaatg
     5761 cctgactgtc tctaccatgt gctcacgaaa cagaacatcg aaaggcgctt caaacttcat
     5821 atcttccttt gtcaacctgg tgaaacctct cccgtcaagc cttgcaacaa tatgcacatc
     5881 aggcacaatt acctggtcca aggactcctc atactttctc atcattttat caaaatcatc
     5941 aaacctcatc tgtccagtcc tccactttga acccagtgtc tgttatgtgt acatagtaaa
     6001 gctcgtcaaa gccttcttca aagcttggaa tttcaagctt cttgtgagtt gcaaacagtg
     6061 ctttatcagg tatgttcttt tcatttcttt tagcgtttct cttaaggcat ccatttatgt
     6121 cggacttgaa atagtagcct ataaccttgt aacccttctc ctttgcaaga ctaatatatc
     6181 tttgtctgtt ttctcttgtt gggtttgtat tgtccacaac gaaacttaag ttattctcaa
     6241 agcagtcttc aagaagcagc ttttctttgt tcctggttct taatgtgtcc aggtttacat
     6301 gcacatactc atctgcaaag ttttccttaa aaaaggtggt tttgccactg gcctgcagcc
     6361 ctgtgaatat aatt
//
LOCUS       DAONVY010000058         6369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9148_length_6369_cov_3.951853, whole genome shotgun sequence.
ACCESSION   DAONVY010000058 DAONVY010000000
VERSION     DAONVY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_6369_cov_3.951853"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="PLI11_02605"
     CDS             complement(<1..1616)
                     /locus_tag="PLI11_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPZ51786.1"
                     /translation="MIGLTSKEAHNRLLKYGKNNIQVKKKVPAIAIFFQQFIDVMTII
                     LMFCTVISAFMGDLVEAVVMISIVVLNAILGFVQEFRTERTIEALRNMSAQKATVIRD
                     GAEIHIPAENIVPGDLVVVNSGDKVPADGKIIESPGVNVDESMLTGESIAVFKEEGTL
                     YGGTLVTSGHAIMEVTETGMKTEMGKISQMIQDVKEEETPLQKRLAAIGKYIVICCIV
                     VCIGVVIVGLVRGQPALEMLLAGISLAVAAVPEGLPAIVTISLALGVQQMAERNALIR
                     KLPAVETLGSTNIICSDKTGTLTQNKMTVRKFVTLGEVFSGTNYEVDEKRLFDISRYA
                     NNHSDATELALIKATEAHGDDRFERIGEIPFDSARKCMSVIVQDKNGARYIFTKGAPD
                     VVVRKSTFYQENGATRKMSTSTQRKIMLHNNQLARDGLRVLAMAWRKLGPGDNLPYDD
                     LEKDLVFVGMAGLIDPPRPEVKDAVALCKKAGIKTIMITGDHKITASVIAKELDIMQK
                     GDRIITGEELERMSDTELENVVEKTAVFARV"
     gene            complement(1789..2334)
                     /locus_tag="PLI11_02610"
     CDS             complement(1789..2334)
                     /locus_tag="PLI11_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51787.1"
                     /translation="MKRNHCGILVMIIAIFLTISFAGCSSPQKEDGKHMDNEEKELYG
                     RIVPPEGFVEGTSETENVMATYKKDMANITLLRTYVGSDETLEEYVRDSREKYEDDVL
                     HFMEFEFVDTDGLEYSGSDKDAIAFEALQDKVMRKYWVVIVLHAEYAFEIICNAPKNE
                     FDEYATSFNAAISSFEIFESK"
     gene            complement(2331..3320)
                     /locus_tag="PLI11_02615"
     CDS             complement(2331..3320)
                     /locus_tag="PLI11_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPZ51788.1"
                     /translation="MVKLKRKFGDRYDGYRVRNVDPFFLLIPYIMKTRVDSQVYFSDR
                     IEITELERFVRKHAGMDIKGLKMYHVLTAAIVRLLSQRPYLNRFVSGNKLYARNHISI
                     SMSVKKNMTEEAETTVVKPMFEPTDSLSDIVRKFSEEVDKNKIIEAQNQTDFTAKLIG
                     SLPSFLIRGVITILHNMDKIGLLPKAINKVSPFHTSVFITNMGSLGIKSIYHHIYEFG
                     TTSIFVAMGKKYSRMELDNDGNAIKRKYIDIKVVADERICDGYYYALSMRMLSKILKN
                     PEILLDKPDTIMLDDGIILKGRLSKEQMESLDDYSYTVPKEDMDIGELKKEII"
     gene            complement(3387..4421)
                     /locus_tag="PLI11_02620"
     CDS             complement(3387..4421)
                     /locus_tag="PLI11_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPZ51789.1"
                     /translation="MKSEEKEIKLRKFIIILAIAAMCLVMATILNECARDLDSLAHKP
                     TSSPTDEPINTATPEASPTPNPTETATEEPTPTPTPTPTPTPTFVPDGEYSQNEKYFG
                     KKVIVLTFDDGPHRIITDRLLDGLKERGVKATFFVTAIGRTNSDWESDKRILNRMIEE
                     GHEIGNHTQEHKRLTEISDEEIREQLGIVHERVKEMTGYDIKIFRPPGGHDNEAVREV
                     ANMPSIKWSVDTRDWEHLNETALMKYAEENDMTYEEARQDRIQYILDDLMRRIDDTEI
                     SYNRIKHGSILLFHDLYDATVDLVLALVDELMESDEYVFLTVSEAIESEGIVPVSGHV
                     YYTIWPRQSV"
     gene            4692..5609
                     /locus_tag="PLI11_02625"
     CDS             4692..5609
                     /locus_tag="PLI11_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51790.1"
                     /translation="MDISKVYCSDYVLDGELDIPEFGFHVKLQGDDLFLGNNSKHRCF
                     RTLRRHFAEKGYKIYAVSCSDDSLQLIIGNCTLCNIQKDLRLFFASYSVFMAMIDNFA
                     EHSALIRMIRSVDDFMNTFFAIAIDGKKDFADCITYQWSGIKAFLTDKWEYGDLISKD
                     EIYMVYNCGKTPYEFAKAVFNRGYPDYQEVLSESKSINMEEDPNQKLINSILADYFKL
                     PENLFSMFPKTREKILSKSLNDENMFGYIVFSLVSKGNSFRKCAGLLKCSYTKVYNAY
                     NNYMFKRTFNNFSEMNKKFKDFIEKNERS"
     gene            5614..5958
                     /locus_tag="PLI11_02630"
     CDS             5614..5958
                     /locus_tag="PLI11_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51791.1"
                     /translation="MKKHFKILLPLLLIVALVSCQQEKKPYVGTWADDNMTIILKDDN
                     TFEIKDKDGAIKGTYKVNDDELSLVVEEVFEENPVNENIKVGDEIKVKFKVENDVFSV
                     TYDGKVYDFPKK"
BASE COUNT         1774 a         1343 c         1103 g         2149 t
ORIGIN      
        1 actcttgcaa atactgctgt tttttcaaca acattttcaa gttctgtatc actcatcctt
       61 tccagttctt ctcccgtaat tattctatcg cccttttgca ttatatccag ttcttttgca
      121 atcacactgg cagtaatctt atggtcgccc gttatcataa ttgtctttat accagccttt
      181 ttgcacaagg caaccgcatc cttgacctcg ggtcttggag gatcaatgag acctgccata
      241 cctacaaaga caagatcctt ttccagatca tcataaggta gattatcacc aggaccgagc
      301 tttctccacg ccatggcaag aaccctcaaa ccgtcccggg caagctggtt gttatgcagc
      361 atgatttttc tttgagttga agttgacatt tttcttgttg ctccgttttc ctgataaaac
      421 gtgcttttcc ttacaaccac atccggagcg ccttttgtga aaatgtaccg agcaccgttt
      481 ttatcctgta ctataacaga catgcatttt ctggctgaat caaaaggtat ttctcctatt
      541 ctttcaaacc tgtcgtctcc atgggcttcc gttgctttaa tcaatgcgag ttctgttgcg
      601 tcactgtgat tgttggcgta tctggatata tcaaagagtc tcttttcgtc cacttcatag
      661 tttgtgcccg aaaaaacttc gcccagtgta acgaattttc tgactgtcat tttattctgg
      721 gtaagtgtac cggttttgtc cgagcagatt atgttggtgc ttcccagggt ctccacagca
      781 ggaagttttc gtataagagc gtttctttct gccatctgct gaactcctag agcaagggag
      841 atggtcacaa ttgcaggcaa tccttccggc accgcagcca cagccaggct tattcccgca
      901 agtagcattt caagtgctgg ctgacctctt acaagaccaa ctatcacaac accaatacaa
      961 acaacaatac agcaaattac aatatattta ccaattgcag caagtctttt ctgtaaagga
     1021 gtttcctctt cctttacatc ctgtatcatt tgcgagattt ttcccatctc ggttttcatt
     1081 cccgtctctg ttacttccat tattgcatgc ccgctggtta caagtgtgcc tccataaaga
     1141 gtaccttcct ccttgaatac agctattgat tccccggtaa gcatggattc gtccacatta
     1201 actcctggcg actcgataat tttaccgtca gcaggcacct tgtctccgga gtttaccaca
     1261 accaaatctc ccggcactat gttttcagca ggtatatgga tttccgctcc gtcacgaata
     1321 accgttgcct tttgagcact catgttcctt aatgcttcta tggttctttc cgttctgaac
     1381 tcctggacaa aacccagtat agcatttaaa acaacaattg atatcatgac aacagcttct
     1441 acgagatctc ccatgaatgc ggatatcacg gtacagaaca taagtattat tgtcataacg
     1501 tcaataaatt gctgaaagaa aatggcaata gcaggcactt ttttctttac ctgaatattg
     1561 tttttcccat acttcaagag tctgttatgc gcttcttttg aagtaagtcc tatcatttta
     1621 tccaccaagt aactcaactc acctttccag tattgttggc ggcagatgcc tcagtgaatt
     1681 ttattcatga caagcaaata ttatacaggt ttgtacaagt aaaattacat aaaaaaagac
     1741 aagcccgtgt tcttgagctt gtcagttaat caatatacaa aagagatatt atttactttc
     1801 aaatatttca aaggaagata ttgctgcatt gaaactggtt gcatattcat caaactcatt
     1861 cttcggagcg ttgcatatta tctcaaaagc atattcagca tgcaatacaa taacgaccca
     1921 gtactttcgc atcactttgt cctgaagagc ttcaaaggct attgcatcct tgtcgctgcc
     1981 tgaatattct agtccatccg tgtccacaaa ctcaaactcc atgaagtgaa gtacatcgtc
     2041 ttcatacttt tccctactgt ccctcacata ctcttcaagc gtttcatcag aaccaacata
     2101 ggtgcgcagt agagtaatgt tggccatgtc ctttttgtac gtcgccataa cattctctgt
     2161 ctcactcgtt ccttccacaa acccttctgg aggaacaatt cttccataca gctctttctc
     2221 ttcattgtcc atatgcttgc catcttcctt ttgtggcgat gagcagcctg caaaacttat
     2281 tgtaagaaag atagctatta tcatcacaag aatgccacaa tgattccttt tcatattatc
     2341 tccttcttta actctcctat atccatgtct tcttttggta cagtatatga atagtcatca
     2401 agactctcca tttgctcttt acttaatctt ccctttaata ttataccatc atccagcatt
     2461 atagtatcag gtttatccaa aagaatttca ggatttttca gaattttaga aagcatcctc
     2521 attgacaaag cgtaataata gccgtcacat atacgttcat ctgccaccac ttttatatca
     2581 atatactttc tttttatagc attaccatca ttgtccaatt ccattcttga atatttcttt
     2641 cccattgcta caaatatcga agtggttcca aattcatata tgtgatggta tattgatttg
     2701 attccaagag agcccatgtt ggttatgaat acacttgtat ggaagggact taccttgttt
     2761 atggcctttg gaagaagacc aatcttgtcc atgttgtgaa gtattgtgat gactcccctg
     2821 attaagaaac ttggaaggct ccctattagt tttgctgtaa aatccgtctg gttctgagct
     2881 tcaattatct tgttcttatc tacttcttca ctgaatttcc gtactatgtc cgaaagtgaa
     2941 tccgtaggct caaacattgg ctttactact gtagtttcag cctcttccgt catgttcttc
     3001 ttcaccgaca tggaaatgct gatgtggttg cgtgcgtata acttgtttcc tgatacaaat
     3061 ctgttcagat aaggtctctg ggataaaagt cttacaatgg cagcggttag aacatggtac
     3121 attttcaatc ctttaatgtc cattcccgca tgtttcctca caaacctctc caattctgtt
     3181 atctctatcc tgtcggaaaa atagacctgg ctgtctactc ttgttttcat tatataaggt
     3241 atgaggagaa aaaacggatc tacatttctt actctatatc cgtcatatct gtctccaaac
     3301 ttccttttta acttaaccat atttttcccc ctaaaaaagt tggtccctaa attatattgc
     3361 actgccattt acactatgtt gattatttac acgctttgac gtggccatat tgtatagtat
     3421 acatgtcctg ataccggaac gattccttct gattctatag cctcgcttac agttaggaat
     3481 acatattcat cggattccat cagctcatca accaaggcaa gaaccaaatc tacagtagca
     3541 tcatacaagt catggaaaag caggattgac ccatgcttta ttctgttgta tgaaatctcc
     3601 gtatcatcta ttcttcgcat tagatcatcc aatatatatt ggattctatc ctgtcttgcc
     3661 tcctcatacg tcatatcatt ttcttctgcg tatttcatca aggcagtctc gttcagatgc
     3721 tcccaatctc ttgtgtccac gctccacttg atggatggca tatttgcaac ctcacgtacg
     3781 gcttcgttat catgcccccc aggcggcctg aaaattttta tatcataacc tgtcatttct
     3841 ttgacacgtt catgtactat tccaagctgt tccctgattt cttcatcgga tatctcagtc
     3901 aaccttttgt gttcctgcgt gtgatttcct atctcatgtc cttcttctat cattctatta
     3961 agtatccttt tatcagattc ccagtctgaa ttagttcttc ctattgctgt tacaaaaaat
     4021 gttgctttaa ctcctctctc cttcaatccg tccaacagcc tgtctgtgat tattctatgc
     4081 ggaccgtcat caaaggttaa cacaataact ttcttcccaa aatacttttc attttgcgag
     4141 tattcgccat caggaacgaa tgttggtgtt ggggttggag taggtgtcgg tgttggagtg
     4201 ggctcttctg ttgcagtctc agttggattc ggtgtagggc ttgcttcagg ggtcgccgta
     4261 ttaatcggct cgtccgtagg tgaacttgtt ggtttatgtg ccaatgagtc caagtctctt
     4321 gcgcattcat ttaaaattgt tgccataaca agacacattg ctgctattgc caaaataatt
     4381 ataaattttc tgagtttgat ttctttttct tcgctcttca tctcatcaat ccttcttttt
     4441 gtatgtaata tatatatatt ataccattag gtacaatatt tcatagtctt tttttgatat
     4501 ataattatcc caatttggta tgtattatat catttgaaaa ttgcatttgt aaatattttt
     4561 aatttattac ctttaatttc aaattatagc atttatgcat ttcgtgtgaa atatattaaa
     4621 attaaaggtt gacaacatat gtttttattt ttaatatata actatcaata acaaaaaaaa
     4681 ggaggaataa agtggatatt agtaaagtgt attgttctga ttatgtttta gacggtgaac
     4741 tggacatacc tgaatttggc tttcatgtaa aacttcaagg cgatgatctc tttttaggaa
     4801 acaactcaaa acatcgttgt ttcagaacct tgaggaggca ttttgcagaa aaaggctata
     4861 agatttatgc agtgagttgt tcagacgatt cgttacaact gattataggc aattgcactc
     4921 tttgcaatat ccaaaaagat cttaggcttt tctttgcaag ttactcggtt tttatggcga
     4981 tgattgataa ttttgccgag cacagtgcgt taattcgcat gataaggtct gtcgacgatt
     5041 tcatgaacac attttttgct attgccattg atggcaagaa agactttgca gattgtatta
     5101 cataccaatg gagcggaatt aaagcattcc tgacagacaa atgggaatac ggagacctga
     5161 tttcaaagga tgaaatctac atggtttata attgcgggaa gactccatac gaatttgcaa
     5221 aggctgtttt caacagggga tatcccgatt atcaagaagt tttatctgag tcaaagagta
     5281 ttaatatgga agaggatcct aatcaaaagc tgatcaattc catactagct gattatttca
     5341 aattaccgga gaatctcttt tcaatgttcc caaagacacg cgagaaaatc ttatccaagt
     5401 ccttgaatga tgaaaatatg tttgggtata tcgtcttttc cctcgtcagc aagggcaatt
     5461 cgtttagaaa atgcgccgga ctcttgaaat gcagctatac taaagtgtat aatgcttata
     5521 acaattatat gtttaaacgt acttttaaca acttcagcga aatgaacaag aagttcaaag
     5581 attttattga gaaaaatgaa aggagctagt tcaatgaaaa agcatttcaa gattttattg
     5641 ccattattgt tgattgttgc tcttgtgtcc tgtcaacaag aaaaaaagcc ctatgtcggc
     5701 acatgggcgg atgacaacat gacgatcatc ctgaaagacg acaatacgtt tgaaataaag
     5761 gacaaggacg gagcaataaa ggggacatac aaggttaatg atgatgagct aagccttgtc
     5821 gtcgaagaag tgtttgagga aaatccggta aacgaaaaca tcaaagtcgg ggatgaaatc
     5881 aaggttaagt tcaaagttga aaatgatgta ttttcagtaa catacgatgg caaagtctac
     5941 gactttccga agaaataaga aaaggggcgg tgcattgttc caatgtgcgg cccttttctt
     6001 atatgtgctt cttttgtgca aaaagtcaat ataagaatgt aattgtttct tgcctctctt
     6061 tatcatttgc ttcacctgcc tgagcgacat gtaatatcgc cgactacaag gccacctttg
     6121 ggtggttttt tctttataaa ggcgcttata tacagacgca tatatacata tgaatctggc
     6181 gaacttgaaa aagcactttt taatttttct catttggtgg tttaattttg catctggaga
     6241 agatcggata tgcggtgcca atatatataa gtagaaaatt aaaaccgtaa aatgaattaa
     6301 gatcgacaat tgacaaccct gtctgccttg atataatcac atcgctattt tatatataaa
     6361 aaggagtta
//
LOCUS       DAONVY010000059         6349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9182_length_6349_cov_3.852876, whole genome shotgun sequence.
ACCESSION   DAONVY010000059 DAONVY010000000
VERSION     DAONVY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9182_length_6349_cov_3.852876"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLI11_02635"
     CDS             complement(<1..630)
                     /locus_tag="PLI11_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPZ51792.1"
                     /translation="MRINESIKDNLILLEKLTLGEKVSLLSSDTPSKLKGVARLGVPS
                     ISFTTPTKGYLRAGQGGFASFDKLDFTCFPSFSLVSCSFNRELLIEMGEAVAKEYLAN
                     EISTFIVSCSVLDSPYYGRGYELFGEDPYVVGELVSAFAVGLQTYGIGAILSDFVIYS
                     GEKQYKNIHIDDFYLHERYLKPFKTVITKTQPLGIMVNSQVINDTQLYTS"
     gene            complement(631..2439)
                     /locus_tag="PLI11_02640"
     CDS             complement(631..2439)
                     /locus_tag="PLI11_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51793.1"
                     /translation="MNIVRFNKCLLMLLGVIFFFQTVHANLKDYETIYVDDMAYAMEN
                     TYIKEEIRFEVPYALSKYNVTRTDDGGYLFLHVDGNRLSSVKYDAEGKCVFGDPLDSG
                     NYKLILEDEEKQVEVLKSRFNLADMQEYNLVNHVACFDDEYFYLPIISTDESIVISMV
                     RFSLVDGMPDYAYKEDLFETEKDNCFSFGAVHAGDGIVVVNIDKYVYEEDNLVSLMHE
                     IWLAGLGFEKKLVYSEEKRIDELEEGAIVRKSAVSHMAVSYENDVYFVEGVMYGEYNK
                     DGEHEVECRVYRLGEDSFAASVNELVEMDYTLENGEYYSVNLLPSNKGLGCIFNFCDV
                     QSDLDSIYTYYSAIILLSYEDMSVQFIAYYPESELVDVNDTEMLRYQNYLDLLEINDE
                     LTTVWEYRMPTEDVIFLGELKSLSFVMLDDEGNVKDTLIMKESLTNAEAIYGCAGTNR
                     LYVFDDIPEYDKYTQVLSPDTIVSFVSMGYGVAYTVTGLTAVVVEVDEEANVIEGLRI
                     NNINIDELSEEVYVEFEEQRLENCMFSHDKEGDYIVTIVVVSSDGREFRFEREVTVIP
                     SSVVNPPTGEPWLALSLVVAIVMPLVLCKTERRKHA"
     gene            complement(2537..3763)
                     /locus_tag="PLI11_02645"
     CDS             complement(2537..3763)
                     /locus_tag="PLI11_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51794.1"
                     /translation="MATNVKVKKKKGTYDNPKLFTYITIGLAAIIILITVIAIVIYTT
                     GNYVGYVNGKKLYDYEYEYYLYLELSEMQAEIDVEGLSDQEKTEKYREFWNTPDENGV
                     YPEEKAKMNALEEARKFKAFYLLAKKEGYKLSKEEQSNIKTNIDFTLQQWMSQYANMG
                     MSVPREALIRNLCGTMTLNQYKKYMIQYATIEKYKDALKETYTISDEEIRNIYNEDRD
                     KYRVVDIRVLFMEAVNEEGEDMTEEEYEELLEKAEEIAKSFNETGKYDDKEFPEYVKE
                     NTDEYTTDGIHRIIKESREHNVEKINEFAYELEKEDFNEDGVTEHVVIEDEDKKGVYI
                     VRGEKIIDIDTEKDEEDENEVSGQDIKDRIIAERKEEIAVEDLEKAVDNETYSVKKVK
                     DKVMQKYVDEWDLRNQ"
     gene            complement(3878..>6349)
                     /gene="mfd"
                     /locus_tag="PLI11_02650"
     CDS             complement(3878..>6349)
                     /gene="mfd"
                     /locus_tag="PLI11_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPZ51795.1"
                     /translation="EDVLVFMDDPDRMEATVNTVIEDHARICDSISEKKGLLSETYGM
                     HMDFPTLDRKTIKFPKVELVSTDSADFTIPSKQVLSFVRNPSLFLEQISTWIDDGYLV
                     VILVGSESKVRRMAEMLVEDGIDPSRVNIMVGGLQSGFEYPDFKLAVISDSSLLKGRT
                     AKRRASLKDGKPIASFAEIKPGDYVVHDTYGIGVFTGLETIEIDNVKRDYIRIKYAGS
                     DSLFIPTDQLSAIQKYIGPEAGSLRLNKLGGSEWKNTTGKVKESLRVYAKELVELYAK
                     RANTKGYAFSKDTVWQKEFEDSFIYEETEDQLKCVEEIKADMEKSMPMERLLCGDVGY
                     GKTEVALRAAFKAICDGKQVAFLVPTTVLAQQHYKNFVERFKDFPVKVEHLSRFRTSA
                     EKNKILRELKKGSIDLLVGTHSIIQRNIEFKDLGLLIIDEEQRFGVMHKEKLKQDWPG
                     VDILTLSATPIPRTLHMSLSGIRDISIIQDPPEARYPVQTYVIEWEEPVIRNAIYREM
                     SRKGQIFYLYNRVRGIEEKRRQLSELVPEARIVVAHGQMSERTLEETMLAFGRGDYDI
                     LLCTTIIESGLDMPNVNTVIVEDGDKLGLAQLYQIRGRVGRSDRLAYAYITYKKDKEL
                     NEIAERRLRTIREFTEFGSGFKIALRDLEIRGAGSVLGEKQHGQLAAVGYDMYCKLLA
                     EVVQEERGIEPSEIKEEISAKVEFQVDAYIDSAYIEDEEARLVMYKKIAGIQSREDIG
                     EVVDELIDRFGDVPESVYNLIQISYIRYLAGKCKFASVVCKNTHVAFVDANNKTAFTL
                     PAKNKKPEELLKETVDVLEGIVGNR"
BASE COUNT         1736 a         1529 c          984 g         2100 t
ORIGIN      
        1 actggtgtat agttgtgtat cattaataac ttgagaattt accattattc ctaacggttg
       61 tgttttggtt atgactgttt tgaatggttt tagatatctt tcgtgcaagt aaaaatcatc
      121 tatatgtata tttttatatt gcttttctcc tgaataaatg acaaagtcgg aaagaattgc
      181 ccctattcca taggtttgaa gtcctacagc gaatgcggaa accagttccc cgacaacata
      241 agggtcctcc ccgaacaatt catatcctct tccataatac ggagaatcaa gaacagaaca
      301 cgatacaata aaagtagaaa tctcgttagc aagatattcc tttgccacag cttcacccat
      361 ttcaataaga agctctctgt taaagctgca actgacaagg ctgaatgagg gaaaacaagt
      421 aaaatccagt ttgtcgaaac tagcaaatcc accctgacca gctcttaaat atcctttggt
      481 tggagtagta aaggatatgg acggcacgcc aagtctggca acccccttca gtttggaggg
      541 ggtatcggat gataatagcg aaaccttttc ccccagtgta agtttttcaa gaagtatgag
      601 attatccttg atcgattcat ttatcctcat ttaggcatgt tttctcctct ccgttttgca
      661 taacacaagc ggcatgacaa tagccacaac cagtgacaat gccagccatg gctccccggt
      721 aggaggattc acaacacttg acggaattac agttacttct ctttcaaacc tgaactctct
      781 accatcactt gaaacaacta caatagtgac gatgtaatct ccttccttat cgtgggaaaa
      841 catgcaattt tcaagccttt gttcttcaaa ttccacataa acttcttctg acaattcatc
      901 aatatttata ttgtttattc ttaaaccttc aattacgttt gcttcttcat caacttcaac
      961 taccactgca gttagtcccg ttacagtata agcaactccg taacccatag atacaaaaga
     1021 tacaatcgta tccggagaaa gaacctgtgt gtatttgtcg tactcaggta tgtcatcaaa
     1081 tacatacaat ctgtttgtcc ctgcacatcc atagatggct tctgcgtttg tcaatgactc
     1141 tttcatgata agggtatcct ttacattacc ctcatcatcc agcataacaa aacttaaact
     1201 cttcaactct cccaaaaaga tgacatcttc tgtcggcatt ctgtactccc aaacagtagt
     1261 cagctcatca ttaatttcta gaaggtcaag gtaattctga tatctcagca tttcagtgtc
     1321 gtttacatca accaattcac tttctggata atatgctatg aattgaacgc tcatatcctc
     1381 atacgataaa agaataattg ccgaataata tgtatatatg ctgtccagat cgctttggac
     1441 gtcgcagaag ttgaatatgc atccaagacc cttgttggac ggcagcaggt ttacggagta
     1501 gtattcacca ttttccaaag tataatccat ctcaacaagc tcgtttacgc ttgctgcaaa
     1561 agaatcctct ccaagcctgt acacccggca ttccacttca tgttcgccat ccttattata
     1621 ttcaccatac ataacacctt ctacaaaata tacgtcgttt tcataagaaa ctgccatatg
     1681 ggaaactgct gactttctga caatcgctcc ctcctcaagc tcatctatcc ttttctcctc
     1741 agagtaaaca agcttctttt caaaacccaa accagcaagc caaatctcat gcattaggga
     1801 aacaaggttg tcttcttcat aaacatattt gtctatattc accacaacaa taccatctcc
     1861 tgcatgaaca gctccaaagg aaaagcaatt gtctttctca gtttcaaaca aatcttcctt
     1921 gtatgcatag tcaggcattc cgtcaaccaa actgaaccta accattgata tgacaatact
     1981 ttcgtcagtt gaaattatcg gcagatagaa atattcatcg tcaaaacaag ccacatgatt
     2041 aacaagatta tactcctgca tatcagcaag attaaatctt gactttaaaa cttccacttg
     2101 tttttcttca tcctcgagta taagtttgta gttgccagaa tcaagagggt ctccaaacac
     2161 acattttccc tctgcgtcat atttaacaga gcttaatcta ttcccatcaa catgaagaaa
     2221 caaatagccc ccatcatctg ttcttgtcac attgtacttt gaaagagcat agggaacttc
     2281 aaatctaatt tcttctttta tatatgtatt ctccattgcg taagccatgt catctacgta
     2341 gatggtttcg tagtctttta aattggcatg aacagtctga aaaaagaaaa taacgccaag
     2401 caacattaat aggcatttat taaatcgaac aatgttcata aggcacccct ttgtcatgaa
     2461 ctatcttaat actaacacaa aacaaaggtt tgtacaagcc caataattaa cccctccatt
     2521 tctggagggg ctttatttac tgatttctca aatcccattc gtcaacgtat ttctgcatta
     2581 ccttgtcttt aacctttttg actgagtaag tttcattgtc aacagccttt tcgaggtctt
     2641 caacggcgat ttcttctttt ctttcagcaa taatcctgtc tttaatatcc tgaccagaca
     2701 cctcgttttc gtcttcctca tccttttcgg tgtcaatatc aataatcttc tcaccgcgaa
     2761 ctatatatac tcctttttta tcctcgtctt caatgacaac atgttctgta acgccgtctt
     2821 cattaaagtc ttccttttcg agctcgtaag caaattcatt tatcttttcg acgttgtgct
     2881 ccctgctctc tttaataatt ctgtggatac catcggttgt atactcatcg gtgttttcct
     2941 tcacatactc tggaaactcc ttatcatcat actttcctgt ctcattgaag gacttggcaa
     3001 tttcctctgc cttttcaagg agttcctcgt attcttcctc tgtcatgtct tcgccttctt
     3061 cattaacagc ttccatgaac aaaactctaa tatcaacgac tctgtacttg tccctgtctt
     3121 cattgtaaat gtttctaatt tcttcatcgc taattgtgta tgtctccttg agagcatcct
     3181 tgtacttctc aatggttgca tactgaatca tgtacttctt gtactggttc aaagtcattg
     3241 tgccgcacaa atttctaatg agagcttctc ttggcacgct cattcccatg ttggcatact
     3301 gactcatcca ctgctgaagc gtaaaatcta tatttgtctt tatattgctc tgctcttctt
     3361 tggacagctt gtacccttct tttttagcca agagatagaa cgccttgaac tttcttgctt
     3421 cttccagtgc attcatcttt gctttttctt caggatatac tccattttca tcaggggtgt
     3481 tccagaactc cctgtatttc tcggtcttct cctggtctga caaaccttcc acgtcaattt
     3541 ccgcctgcat ctcagacaac tcaagatata aatagtattc atattcataa tcgtataatt
     3601 tctttccatt aacgtatcct acataatttc ccgtcgtata tattacaata gcaataactg
     3661 ttattagtat gataattgca gccaaaccaa tcgtaatgta cgtaaagagt ttgggattgt
     3721 catatgttcc ttttttcttt ttgaccttta cattcgtagc cattatattc tctaccgccc
     3781 tctaaactta tttaatgcag aattgccttt aagattatac tacttaaaaa cgaatatatc
     3841 aataaatatt ttataaactt tgaataatga ttaaaaatca tctattacca actattcctt
     3901 caagcacgtc aacagtttcc tttaaaagct cctcaggctt tttgtttttg gcaggaaggg
     3961 taaaggcagt tttgttgttt gcatcaacaa aggcaacatg agtgttctta catacaacgg
     4021 atgcaaattt gcactttccg gcaagatagc ggatatatga gatttgaata agattgtaaa
     4081 cgctctcagg cacatctcca aacctgtcta tgagttcgtc tacaacctct ccaatatctt
     4141 ccctgctttg tatgcctgca attttcttgt acattacaag tcttgcctct tcgtcttcaa
     4201 tgtacgcaga gtcaatatat gcatccacct gaaactctac tttggcactg atttcctcct
     4261 tgatttcaga aggctcgata cctcgctctt cctgaacaac ctctgcaagc agcttgcaat
     4321 acatgtcgta accgaccgct gccagctgtc catgctgttt ttcaccaaga acacttccag
     4381 ctccccttat ttccaagtcc ctcaatgcaa tcttgaatcc ggatccaaac tcggtaaact
     4441 ccctaatggt cctaagtctt ctttcggcaa tctcattcag ttccttgtct ttcttgtagg
     4501 tgatataagc ataggcaagt ctgtccgatc ttcctactct tccccttatc tgatacaact
     4561 gggcaagtcc aagcttgtca ccgtcttcca caattacagt attgacgttt ggcatatcaa
     4621 gtcctgattc aattattgtg gtacacaaaa ggatatcata atcacccctg ccaaaggcca
     4681 gcatggtttc ctcgagggtc ctttccgaca tctgaccatg ggcaactaca atccttgctt
     4741 caggaacaag ctctgacagc tgtcttcttt tttcttcaat accccttacc ctgttgtaca
     4801 ggtagaatat ctgacccttt cggctcatct ccctgtatat tgcattacgt ataacaggct
     4861 cttcccactc aatgacatat gtttgcacag gatatctggc ttcgggagga tcctgtatga
     4921 tgctgatatc ccttatgccg gaaagggaca tgtgaagagt tcttggtatt ggtgttgcag
     4981 acaatgtcaa aatatccaca cccggccagt cttgcttgag cttttctttg tgcatgacac
     5041 caaaacgctg ctcctcatct ataatcaaca gacccaaatc cttaaattct atgtttcgtt
     5101 gtataatact gtgggttcca accaatagat ctatactgcc tttttttaat tctcttaata
     5161 ttttgttttt ctcggcgctt gtcctgaatc tgctcaaatg ctcaactttt acaggaaaat
     5221 ccttgaatct ttctacaaag ttcttgtaat gctgctgtgc aagtacggtt gttggtacta
     5281 aaaatgccac ttgtttgcca tcgcatattg ctttaaatgc agctcttaaa gcaacttctg
     5341 tttttccata acccacatct ccgcaaagaa gcctttccat tggcatggac ttttccatgt
     5401 cggcctttat ctcttccacg catttcagct ggtcttcagt ctcttcgtat ataaagctgt
     5461 cttcaaactc cttttgccaa accgtatcct ttgaaaaggc ataccctttt gtatttgccc
     5521 ttttagcata aagctcaacc aattcctttg catataccct cagtgattcc tttactttgc
     5581 cggtggtatt cttccactcg cttccaccaa gcttgttaag tcttaaagaa cccgcttcag
     5641 gtccaatata tttttgtatc gcagacagct ggtctgtggg tatgaacaat gaatcgctgc
     5701 ctgcatattt tattctgata taatctcttt ttacattatc tatttctatt gtctcaagac
     5761 ccgtaaatac tcctattcca taagtatcat ggacaacata gtccccaggc tttatttctg
     5821 caaaacttgc tatgggtttt ccatccttaa gggatgctcg tctttttgca gttcttcctt
     5881 tcagcaatga actgtcgctg ataacagcaa gcttgaagtc cggatattca aatcccgatt
     5941 gaagtcctcc caccattatg ttaaccctgg aaggatcaat accgtcttca acaagcattt
     6001 ccgccatgcg tcgtactttg ctctcggaac ctaccaaaat cacaacaagg taaccgtcat
     6061 caatccatgt actaatctgc tcaagaaaca atgaaggatt cctgacaaaa gacagaacct
     6121 gcttcgatgg aatggtgaaa tctgcactgt cggttgaaac tagttccact ttaggaaatt
     6181 tgattgtctt cctatccaac gttggaaaat ccatatgcat gccatacgtt tctgagagaa
     6241 gacctttttt ctcgcttatg gagtcgcaaa tccttgcatg gtcttcgatt actgtattga
     6301 ctgtagcctc cattctgtcc ggatcatcca taaatacgag aacatcttc
//
LOCUS       DAONVY010000060         6286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9298_length_6286_cov_3.694592, whole genome shotgun sequence.
ACCESSION   DAONVY010000060 DAONVY010000000
VERSION     DAONVY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9298_length_6286_cov_3.694592"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PLI11_02655"
     CDS             complement(<1..1252)
                     /locus_tag="PLI11_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HPZ51796.1"
                     /translation="MNIFDKITYLQEKSHGYHAIRATTQLRYAYRISEVNDFKYNDIV
                     LKAADVLLGDLNINGSITKNAAKECEDILSVLSEEAKKYTMICIAHAHIDMNWMWSYN
                     ETVAITLDTFRTMLKMLREYPQFTFSQSQASTYKIVEKHDPEMFEEIKEMVKMGRWEV
                     TASTWVEGDKNMTSGESQARHILYTKKYFKEKFGIPFDSILLDYEPDTFGHNAQVPEI
                     LEKGGIKYYYHCRGSESHCIYNWKSPSGASVLVFREPDWYNTIITYDYADYLPSFCKE
                     HDIDRLIRVYGVGDHGGGPTRRDIERLIDMSTWPCYAKIEFGSYRTFFEYLETKRENF
                     PTVEGELNFVFTGCYSSESRIKMANRMAEDSLNTAETLTALANAFANGKPYTKGYTKA
                     WIKTLFNHFHDILPGSGIRETREHA"
     gene            1377..1976
                     /locus_tag="PLI11_02660"
     CDS             1377..1976
                     /locus_tag="PLI11_02660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPZ51797.1"
                     /translation="MSNIDATIQYYNQNAKDFYTDTVSGDMSTLLDRFLSYLEENAYI
                     LDFGCGSGRDTKYFLEKGYLLDAVDGSAEMCRIASGLTGIEVRHMYFHELEEKDKYDG
                     IWACASILHVPYESMVAILQKLYEALKDDGVLYLSFKYGDFEGMRNGRWFTNMNEDRI
                     NRLLCDVPLFVVMEMWTTQDVRPDRKDEKWLNCILKKRK"
     gene            1943..4372
                     /locus_tag="PLI11_02665"
     CDS             1943..4372
                     /locus_tag="PLI11_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPZ51798.1"
                     /translation="MAELYFEKEEVKVVTNTENTMEKHLVKSLDSADRVDIIVSFVME
                     SGVRLLLTDLKSIVQKGVKVRILTGDYLGITEPGALYLLKGELGGNLDLRMYNDKRRS
                     FHPKTYIFHKRKDSELYIGSSNLSISALTDGVEWNYRLSESVDKESFDEFVKEFENLF
                     YNHSIIVDDEVLREYSRNWKRPLIIGELEKNEKKTREYVPRGAQIEALYALKATREEG
                     ADKALIHAATGVGKTYLAAFDSLSFKRVLFVAHREEILRQAYESFRNVRNEEDFGFFN
                     SKIKDCDRNVVFASVYSLGRKNYLNEKFYSPTYFDYIIIDEFHHAVANLYMNILEYFK
                     PKFLLGLTATPERMDGRSIYALCDYNVPYEIGLKDAINKGYLVPFRYYGIYDDTVDYS
                     TIRIANGRYVAEELEDKLLVERRYDLIYGHYKKHNSIRAMGFCSTRKHAEEMAKQFNK
                     RGTASVAVYSNGNGEYSEDRYAAMEKLEKGEISVVFSVDMFNEGVDICSIDMVMFLRP
                     TESPVVFLQQLGRGLRKSEGKEYLTVLDFIGNYALCGKVPFLLSGQKYDKEIALTGLV
                     EHKLEYPQGCFVNFDLKIIDLFREMSRREIGIEDRIILEYERVKGLTGRDKLSRMEFF
                     TNMDGDVYTLCMNNSKVNPFRDFLAFLDKMGELGEEEKMLVSGIGKEFLNTLETTSMT
                     KSYKMPILLAFYNDGNTKMDLTEDDVYKSYKSFYEKGTRWVDLARDKRTRDFTAWNKD
                     RYIKEALRNPIFYLIKSGNGFFAEKEGYLISLNSQLEELTTLQAFAKHMKDIIDYITA
                     DYFRKRYDKNS"
     gene            4529..5032
                     /gene="purE"
                     /locus_tag="PLI11_02670"
     CDS             4529..5032
                     /gene="purE"
                     /locus_tag="PLI11_02670"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPZ51799.1"
                     /translation="MAEPKVLVVMGSDSDYEIMKSCLKILRKFEIGFECMVCSAHRTP
                     DEAANLAKNAIENGFDAIIAAAGKAAHLPGVIAAYTTLPVIGVPIVSGELDGMDALLS
                     IVQMPPGIPVASVAINGALNAGILACEILSLKYDNLKKVLLEYKNEMKKKVEEKNNAL
                     QQKIADL"
     gene            5042..6142
                     /gene="purM"
                     /locus_tag="PLI11_02675"
     CDS             5042..6142
                     /gene="purM"
                     /locus_tag="PLI11_02675"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPZ51800.1"
                     /translation="MSSYRDAGVDIEAGYEAVRLMKDHVKRTYRPEVISDIGGFGALF
                     QLDLSKYEEPVLVSGTDGVGTKLRIAFIMNKVDTIGIDCVAMCVNDIVCSGAEPLYFL
                     DYIAVGKNQPAVIADIVKGVADGCVQAGCALIGGETAEMPGFYNNREFEIAGFAVGVV
                     SKSKIINGKDIKAGDKLIGLASSGLHSNGYSLVRKVFNPSFERMNEFVTALNCTLAEE
                     LLRPTRIYVKTVLDMIEKFNVKGISHITGGGFFENIPRMSANDVVYHINKGSWDVLPI
                     FGMIQRLGHVTEPEMFNTFNMGIGLCMAVDAKDVDKVMAYLEEIGERAFVIGEVKNMD
                     KAKLEEEYGLDESSDEEKMEYEVLYKNGVLIC"
     gene            6136..>6286
                     /locus_tag="PLI11_02680"
     CDS             6136..>6286
                     /locus_tag="PLI11_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPZ51801.1"
                     /translation="MLRVGVLVSGGGTNLQAIFDKIESGYLSNCTIVTVVSSNEKAYA
                     LKRARK"
BASE COUNT         1875 a         1025 c         1516 g         1870 t
ORIGIN      
        1 gtgcatgctc ccttgtttcc cttatgccag aaccgggcag aatgtcatgg aaatgattga
       61 agagagtttt tatccatgct tttgtgtaac cttttgtgta gggttttccg ttggcaaacg
      121 cattggcaag tgcagtcaag gtttctgctg tattcagact gtcttccgcc attctgttgg
      181 ccattttgat tctggattcg gaactgtagc atcctgtgaa tacgaagttg agttcacctt
      241 ccacagtcgg gaagttttct ctttttgttt ccagatattc aaagaaggtg cggtaggagc
      301 caaactctat ttttgcatag catggccatg tactcatgtc tatcaagcgc tctatgtctc
      361 ttcttgtagg accaccgcca tggtctccaa caccgtaaac ccttatcaat ctgtcaatgt
      421 cgtgttcttt gcagaaggag ggcaggtagt ctgcataatc ataagttatt atggtgttgt
      481 accagtcggg ttccctgaag acaagcacac ttgctcctga tggactcttc cagttgtaga
      541 tgcagtggga ttcgctgcct ctgcagtggt agtagtattt gattccgcct ttttcaagta
      601 tttcaggaac ctgggcgttg tgtccgaagg tgtcgggttc ataatcaaga agaattgaat
      661 caaacggaat tccaaatttc tctttgaagt atttctttgt gtaaaggata tgtcttgcct
      721 ggctttcacc gctggtcata ttcttgtcgc cttcaaccca ggtggatgcg gtgacttccc
      781 atcttcccat ctttaccatt tcctttattt cctcaaacat ctcagggtca tgtttttcca
      841 caatcttgta tgtggaagcc tgggactggg agaaagtgaa ctggggatat tctctaagca
      901 ttttcagcat tgtacggaaa gtatccaaag taatggccac tgtctcgtta tagctccaca
      961 tccagttcat gtctatgtgg gcatgggcaa tgcaaatcat tgtgtatttt ttagcttctt
     1021 ctgataaaac cgaaagaatg tcttcacatt cttttgcagc atttttcgta attgaaccat
     1081 ttatgtttaa atctcccaaa agtacgtcag cggcttttaa gactatgtcg ttgtatttaa
     1141 aatcattgac ttcagaaatg cgatatgcat atcttagctg agttgtcgcc ctgattgcgt
     1201 gataaccatg ggatttttcc tgcaaatacg taattttatc aaatatgttc atgccatctt
     1261 cctccttatt ctttgaacta atattattga attaataaag aaatgtcaaa gcttttattt
     1321 gctctgtttg tgtgatataa tttccaggaa tatggatgcg aggttcgaat atgaatatgt
     1381 caaacattga tgcaacaatt caatattaca accaaaatgc caaagatttt tatactgata
     1441 ccgtatccgg ggatatgagc actcttcttg acaggtttct ttcatacctc gaagaaaatg
     1501 catacatact tgactttggc tgtggctcgg gaagggatac aaaatacttt ttggaaaaag
     1561 gttatttatt ggatgctgtt gacggttcgg ctgagatgtg caggattgca tccggattga
     1621 ccggtattga ggtaagacac atgtatttcc atgagcttga ggaaaaagac aagtatgatg
     1681 gcatatgggc atgtgcgtcc atactgcatg ttccgtatga aagcatggtt gccatattgc
     1741 aaaagttgta tgaagcattg aaagatgacg gtgtattgta cttgtccttt aaatatgggg
     1801 atttcgaagg aatgcgtaat ggaagatggt ttaccaatat gaatgaggat agaataaaca
     1861 ggctgctttg tgatgtccct ttatttgttg ttatggaaat gtggactacc caggatgtac
     1921 ggccggacag gaaagatgaa aaatggctga attgtatttt gaaaaagagg aagtaaaggt
     1981 tgttacaaac actgaaaaca ccatggaaaa gcatctcgta aaaagccttg attcagctga
     2041 cagggttgac ataatcgtct cttttgtcat ggaatccggg gtcaggcttc ttttgacaga
     2101 tttgaagagt attgttcaaa aaggtgtaaa ggtacgtatt cttactggag actacctggg
     2161 gattacagag cctggggcgt tgtatttgtt gaagggagag cttgggggta atcttgattt
     2221 gcggatgtac aatgacaaaa ggcgttcatt tcatcccaaa acatacatat ttcacaaaag
     2281 aaaagacagt gagctgtata ttggttcttc caatcttagt atcagtgcat taaccgatgg
     2341 tgtagagtgg aattacaggc tttctgaaag tgtggataaa gagagttttg acgagtttgt
     2401 aaaggaattc gaaaatctat tttacaatca ctcaataatt gttgatgatg aagtacttag
     2461 agagtactca aggaattgga aaaggccatt gataattggg gaattggaaa agaacgaaaa
     2521 gaaaaccaga gagtatgtgc cgagaggagc acagattgaa gccttgtatg ccctaaaagc
     2581 aacaagagaa gagggggcgg acaaggcttt aattcatgca gctacaggtg ttggaaaaac
     2641 atatcttgcg gcctttgatt cgctcagctt caaaagggtg ttgtttgttg cccataggga
     2701 agagatatta agacaggcat atgagagttt taggaatgta aggaatgagg aggattttgg
     2761 ctttttcaac tcaaaaatca aggattgcga tcgaaatgtt gtttttgctt cggtttacag
     2821 tttgggaagg aaaaattatt tgaatgaaaa attctattca cccacctatt ttgactatat
     2881 tattatcgac gagtttcatc atgctgtagc aaatttgtac atgaatattc ttgaatactt
     2941 caaacctaag tttcttttgg gtttgacagc tactcctgaa agaatggatg gaagaagcat
     3001 atatgccttg tgtgattaca atgtgccata tgaaattggt ttgaaggatg ccataaacaa
     3061 aggatatctc gtgccttttc gatattatgg gatttatgat gatacggttg attattccac
     3121 catcaggatt gcaaatggca gatacgtggc ggaagaactg gaagacaagc ttttggttga
     3181 aagacggtat gatttgatat atgggcacta caagaagcat aattccataa gggctatggg
     3241 tttttgcagt acaagaaaac atgccgagga aatggcaaag caattcaaca aaagggggac
     3301 tgcgtctgtt gctgtataca gtaatggcaa tggtgagtac tctgaggaca ggtatgcagc
     3361 aatggaaaag cttgaaaagg gtgagataag cgttgtcttt tccgtagaca tgtttaatga
     3421 gggtgttgat atttgctcaa tagacatggt catgtttttg aggccgacgg agtctcctgt
     3481 cgtttttttg cagcagcttg gcagggggct tagaaagagt gagggaaaag agtatttaac
     3541 cgtccttgat tttattggca attatgctct ttgtgggaaa gtgccttttt tgctgagcgg
     3601 acagaaatat gataaggaaa ttgcactaac aggccttgtg gaacataagc ttgagtatcc
     3661 gcaaggctgc tttgtcaatt ttgatcttaa aattattgat ttgtttaggg aaatgtcaag
     3721 gcgggaaatt ggtattgaag acaggattat tctcgaatac gagagagtca aaggattaac
     3781 aggtcgggac aagttgtcca ggatggagtt tttcacaaac atggatggag atgtatacac
     3841 tctatgtatg aataattcaa aagtgaatcc gttcagggac tttcttgctt ttttggacaa
     3901 aatgggtgag cttggcgaag aggagaagat gcttgtcagt ggtattggaa aggagtttct
     3961 taatacactg gaaacaacaa gcatgacaaa gtcgtacaag atgccaattc tgcttgcctt
     4021 ttacaatgac gggaacacta aaatggattt gactgaggat gatgtgtaca aaagttacaa
     4081 aagtttttat gaaaaaggca cgagatgggt ggatcttgcc agggacaaga gaacaaggga
     4141 ttttactgct tggaacaagg atagatacat aaaggaagcg cttagaaatc caatatttta
     4201 tctcattaaa tccggaaatg gattttttgc agagaaggaa gggtatttga tttcactaaa
     4261 cagtcaattg gaggaactga caacgttgca agcttttgcg aaacacatga aggatatcat
     4321 tgattacata acggctgatt atttcagaaa aaggtatgat aaaaacagtt gaattaaaga
     4381 aatattgagt ttcaaaattt tttagttgca ttttatacat agcttaggtc tataacattc
     4441 ttgcaaacaa aaaaatgcta tgctataatg acagcggatt cggaattatt ccgtatccgt
     4501 tatttcatac caaatcggag gataacaaat ggctgaacca aaagtgctgg ttgtaatggg
     4561 cagtgattct gattatgaaa ttatgaaaag ttgtctcaaa attctaagaa aattcgaaat
     4621 cggctttgaa tgcatggtgt gttcagccca cagaacacct gatgaagctg caaatctcgc
     4681 caaaaacgca attgaaaacg gttttgacgc aattattgcc gcagcaggca aggctgcgca
     4741 tttgcctggt gtgatagcgg cgtatacaac tcttcctgtt ataggtgttc ccatcgttag
     4801 tggagaattg gatggaatgg acgctttgtt gtccatagtt caaatgccgc caggtattcc
     4861 agtagccagt gttgcaataa acggcgcatt gaatgcggga attcttgcat gtgaaatatt
     4921 gtcgctgaaa tatgataatc ttaagaaggt tcttttggaa tacaagaatg aaatgaaaaa
     4981 gaaggttgaa gagaaaaaca atgctctaca acaaaagata gctgatcttt aaggaggaaa
     5041 catgagtagt tacagagatg caggcgttga tattgaggcg ggttatgagg ctgttagatt
     5101 gatgaaagat catgtaaagc gcacctacag gcctgaggtc atttccgata taggcggttt
     5161 tggtgcactg tttcagctgg atttaagcaa gtacgaggaa cccgtactgg tttccggcac
     5221 tgatggcgtt ggtaccaagc tcagaatcgc ctttataatg aacaaggtag atactatagg
     5281 aatcgactgc gttgccatgt gcgtcaacga catagtgtgc agcggcgcag agcctctgta
     5341 tttcctcgac tatatcgcag tgggcaagaa tcagcctgcg gttattgcgg atattgtcaa
     5401 aggcgtagcc gatggatgtg ttcaggcagg ttgtgcgctg ataggcggag aaactgctga
     5461 aatgcccgga ttctacaaca acagggagtt tgaaatagct ggttttgctg tcggagttgt
     5521 ctccaagtct aaaatcatta atggaaaaga tataaaggcg ggggacaaac tgataggctt
     5581 ggcatcgtcc ggtcttcaca gcaacggata ttctcttgta aggaaagtgt ttaatccaag
     5641 ctttgagaga atgaatgagt ttgtcacagc gttaaactgc acattggcgg aagaactttt
     5701 aagacctacc aggatttatg taaagactgt cctggatatg attgaaaagt tcaatgtcaa
     5761 aggcatctcc catattacag gtggaggatt ctttgagaat attccaagaa tgagtgcaaa
     5821 tgacgtggtt taccacatta acaagggttc atgggacgtt cttccaatat ttggaatgat
     5881 tcaaagactt ggtcatgtaa ccgagcctga gatgtttaat accttcaaca tgggtattgg
     5941 tctttgcatg gctgttgatg ccaaggatgt ggataaagta atggcctacc ttgaggaaat
     6001 cggggaaagg gcttttgtaa taggcgaggt aaaaaacatg gacaaggcaa aacttgaaga
     6061 agagtatggt cttgatgaat cctcagatga agaaaagatg gaatatgaag tcctatataa
     6121 aaatggtgtg ctgatatgtt gagagtaggt gttttggtat ccggaggagg tacaaatctc
     6181 caggcaattt ttgataaaat tgaatccgga tacctttcca attgcacaat agtcacagta
     6241 gtatcaagta acgagaaagc ttatgcgctg aaacgtgcta gaaagc
//
LOCUS       DAONVY010000061         6277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9315_length_6277_cov_3.783671, whole genome shotgun sequence.
ACCESSION   DAONVY010000061 DAONVY010000000
VERSION     DAONVY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_6277_cov_3.783671"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLI11_02685"
     CDS             <1..404
                     /locus_tag="PLI11_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPZ51802.1"
                     /translation="VILVILAEIYRTRKILAGLGGGLYSLYNTFSGYVSDILSYSRLL
                     ALGLCTGSVAGVVNMVGVLPSNPVVKAVSLTIAFVLGHSLNFAINALGAYVHTNRLMF
                     IELFSKFYEGGGRAFKPFRINTAYVKIKEEI"
     gene            407..880
                     /locus_tag="PLI11_02690"
     CDS             407..880
                     /locus_tag="PLI11_02690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPZ51803.1"
                     /translation="MDKYGIVIAVLGAISAALFSGMGSAKATGMVGESAAGVISEDPS
                     KFSKLLILQLLPGTQGLYGLLVAVLALSKLGLLAGEIQAITWGQGLMYFFACMPITVV
                     GYWSAIKQGRTAIAGVGIVAKKPEQSGRAVTLAAIVEIYAILALLISILALNGIG"
     gene            892..1497
                     /locus_tag="PLI11_02695"
     CDS             892..1497
                     /locus_tag="PLI11_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPZ51804.1"
                     /translation="MDTGLEKILKKIEEDCDAEIKRIIDAAEREANEFYCDAEKEALS
                     QKEKRFEKAKSDSKARISIAVKTFELEKRNMLLKAKNQLIDEAIDMAVERARNLKDED
                     FLSLVKNLILKHSLKGEGVLRFSEKDSPRITDFFLNEVNSILKNAGKHVEKGESIDIK
                     DGFVIEYRDVNINCTYGALLKGMEDEIREHVHRVLFQEDIE"
     gene            1494..2459
                     /locus_tag="PLI11_02700"
     CDS             1494..2459
                     /locus_tag="PLI11_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPZ51805.1"
                     /translation="MRETDYAYSVGVIRYRETYLISKSDMDALVDMPDIESSLSFLDS
                     KGYKDISLENLNAYLNQKSKEVWDFLVETVPNSTVFSFYLLKNDFHNLKVSLKSKVLG
                     VETRDFLQPSCISPEFLKERVDEKDFDNLSYGLKECAIEAYDILVRTKDGFLCDLVVD
                     RHCLDAVLKEVQKTRNEFLISYANTFASFSTIKTAYRLAGGRRDKEIINMAVCHVSEP
                     DRVELVEACAKSKEALISYLKSTKFNDIAKYLEKSVVVYEKECDERLIGMCMDVKDRV
                     LGPEPIFAYYMAKEMEIANIRIILYCMRNGIDKETIRERVRNIYV"
     gene            2452..2760
                     /locus_tag="PLI11_02705"
     CDS             2452..2760
                     /locus_tag="PLI11_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPZ51806.1"
                     /translation="MYKVAVMGDKDSVEGFKAIGMHAVVANKEDAQLKLKELVELNYA
                     AIFITEELASGIPEVIERYKEEIIPAIILVPGLKGNTGDGMRNIGELVKKATGADILG
                     "
     gene            2774..>3928
                     /locus_tag="PLI11_02710"
     CDS             2774..>3928
                     /locus_tag="PLI11_02710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPZ51807.1"
                     /translation="MNHGTIVKVSGPLVVAEGMRHASMFDVVRVGKANLIGEVIEMHG
                     DRASIQVYEETEGLGPGDVVVSTNLPMSVELGPGLIGSIFDGIQRPLDEIMKISGHNL
                     TRGVMVPSLNRERKWNFVKCVNEGAYVEAGDVLGTVTETEVVVHKIMVPYGVSGTILK
                     IEEGSFTVTDTIAVVETEKGKVELAMMQTWPVRKERPYQKKLPPEMPMVTGQRVIDCL
                     FPVAKGGVAAIPGPFGSGKTITQHQLAKWAEADIVVYIGCGERGNEMTDVLMEFPKLV
                     DPRTGKSIMERTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRWA
                     EALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGRIVSLGSEKREGTLTA"
     assembly_gap    3929..3938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3939..4545
                     /locus_tag="PLI11_02715"
     CDS             <3939..4545
                     /locus_tag="PLI11_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPZ51808.1"
                     /translation="AVSPPGGDFSEPVTQATLRIVKVFWGLDANLAYKRHFPAINYLT
                     SYSLYVDTLEKWFDENISPLWCKLRIRMLQTLQDEAELEEIVKLVGMDSLSPQDRLKM
                     EVARSIREDFLHQLAFHEVDTYTSLEKQFLLMRLIFEYYDEAVILLKDGGNIEEIVNI
                     PVREAIGRFKYVKEEEIHARYADISEKIRNELKALAGKEEI"
     gene            4545..5909
                     /locus_tag="PLI11_02720"
     CDS             4545..5909
                     /locus_tag="PLI11_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPZ51809.1"
                     /translation="MPVEYKTIEEVAGPLMLVRDVENVTYNEMGEIELANGEIRRCRV
                     LEVDGSNALVQLFESSIGINLSNSKVRFHGKPMEFGVSEDILGRVFDGLGNPIDGGPP
                     IIPEKRMNINGLPMNPVARNYPSEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHSKL
                     AAQIARQAKTRGAEEPFAVVFAAIGITFEEANFFIESFKESGAIDRTVLFVNLANDPA
                     IERISTPRLALTASEYLAFEKGMHVLVILTDITNYADALREVSAAKKEIPGRRGYPGY
                     MYTDLATLYERAGRQKSKPGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     RKNILPPVDVLPSLSRLKDKGIGEGKTRHDHSDTMNQLFAAYARGKDARELMMVLGES
                     ALTDMDKLYAEFAEAFEREYVSQGFETDRTIEETLDLGWKLLRMLPRTELTRIREENL
                     EKYY"
     gene            5926..>6277
                     /locus_tag="PLI11_02725"
     CDS             5926..>6277
                     /locus_tag="PLI11_02725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPZ51810.1"
                     /translation="MAILAVNPTRMELSKLKKKLTVARRGHKLLKDKCDELMRQFLLL
                     VKENKALRMEVEEGIRNAHKSMIVARSVMKEEEISNALMVSKQEIQVEKKERSIMSVA
                     IPVFETSFKTENEQD"
BASE COUNT         1958 a         1064 c         1598 g         1647 t
ORIGIN      
        1 gagtaatact tgtcatactt gctgaaatat acaggacaag aaagatactt gcagggctgg
       61 gtggtggact ttacagtctt tacaatactt tttccgggta tgtaagcgac atcctttcat
      121 attcaagact tcttgcactg ggtctttgta cgggaagtgt tgcgggtgtt gttaacatgg
      181 tgggggttct accctccaat cctgtagtaa aggccgtttc gctgactata gcgtttgtgc
      241 tgggacacag tctaaacttt gcaataaatg cattaggcgc ttatgtacac acaaacagac
      301 ttatgtttat agagctcttt tccaagtttt acgaaggtgg aggcagagcc ttcaaacctt
      361 ttagaataaa taccgcatat gtaaagatta aggaggaaat ttaacaatgg acaaatatgg
      421 aatcgttata gctgttttgg gagcaatatc agcagcgctc ttttctggaa tgggttcagc
      481 aaaagctact ggaatggtgg gagaatccgc agctggtgta atatctgaag acccttccaa
      541 gtttagcaaa ctcttgatac ttcagcttct tccaggaacc caaggtctct atggtctatt
      601 ggttgcagtt ctggctctaa gcaaactggg acttctggca ggtgagattc aggcaataac
      661 ctggggtcag ggtcttatgt acttctttgc atgcatgcct attactgttg tgggatattg
      721 gtccgcaatc aagcagggaa gaactgcgat tgcaggcgtt ggtattgtgg caaagaaacc
      781 tgagcaaagc ggaagagctg taactcttgc ggctattgtt gagatttacg ccatattggc
      841 gcttctaata tccatacttg cattgaatgg aataggataa gggatggaaa tatggataca
      901 ggtcttgaga agattctgaa aaagattgaa gaggattgtg acgcggaaat aaaaagaatt
      961 attgatgctg ccgaaaggga agccaatgag ttttattgcg atgctgagaa ggaagcttta
     1021 agtcagaagg aaaagaggtt tgaaaaggcc aaatctgaca gtaaagccag gataagcatt
     1081 gcagtaaaga cctttgagct tgagaaaaga aacatgcttc ttaaggctaa aaatcagctt
     1141 attgatgaag ctatagatat ggcggtagaa agggcaagaa atcttaaaga tgaggatttt
     1201 ctttcccttg ttaaaaacct cattctcaaa cattctttga aaggtgaggg agtattgagg
     1261 ttttcagaaa aagattcgcc aagaattaca gattttttcc taaacgaggt aaactcgata
     1321 ctaaaaaatg ccggaaagca tgttgaaaaa ggggagagta ttgacattaa ggacggcttt
     1381 gtcattgagt acagggatgt gaatataaac tgtacatatg gggctttgtt aaaaggtatg
     1441 gaagatgaaa tcagagagca tgtacataga gttctttttc aggaggatat tgaatgaggg
     1501 aaactgatta tgcatacagc gtgggagtga tacgctatcg ggaaacgtat ctcatatcaa
     1561 aatcagacat ggatgccttg gttgacatgc cggatattga aagtagcttg tcttttctcg
     1621 attcaaaggg ttacaaggac atatcccttg aaaaccttaa tgcatattta aaccaaaaat
     1681 caaaagaggt ttgggacttt cttgtcgaga cggttccgaa tagcacagtg ttttcctttt
     1741 atctattaaa gaatgacttc cacaacctga aggtttccct taaatcaaaa gtgctgggtg
     1801 ttgaaacaag ggacttttta caaccctcct gtatttctcc tgagttttta aaggaaagag
     1861 tggatgaaaa ggattttgac aatctttctt atggcttgaa ggaatgtgct atcgaggcgt
     1921 acgatatcct tgttagaacc aaagacggtt tcctgtgtga tctcgtggtt gacagacatt
     1981 gtcttgatgc agttcttaaa gaagtccaaa aaacaaggaa tgagtttttg atatcgtacg
     2041 caaatacatt tgcttccttt tcaactatca agacggcata cagacttgca gggggaagaa
     2101 gggataagga gataatcaac atggctgtat gtcatgtgtc cgaacctgac agagtggagc
     2161 ttgtagaagc ttgtgcaaaa agtaaagagg cgcttatatc gtatttgaag tcgaccaaat
     2221 ttaatgacat tgccaaatat ttagaaaagt ccgtagtcgt ttatgaaaag gaatgtgacg
     2281 aaagacttat aggtatgtgc atggatgtaa aggaccgggt cttgggtcct gaaccaattt
     2341 ttgcgtatta catggctaaa gaaatggaga ttgccaatat tcgcatcata ctttactgca
     2401 tgcggaatgg tattgacaag gagacaataa gggagagggt gcgaaacata tatgtataaa
     2461 gtggctgtaa tgggagacaa ggatagtgtt gaaggattta aggccattgg catgcatgca
     2521 gttgtggcta ataaagagga tgctcaattg aaactaaagg agctggttga attaaattat
     2581 gccgcaatat ttatcaccga agaactcgca tccggtattc ccgaggtaat agagaggtat
     2641 aaagaggaaa taattcctgc cataatcctt gttcctggac ttaaaggaaa taccggggat
     2701 gggatgagga atattggaga acttgtgaaa aaagcaaccg gagcagacat acttggttaa
     2761 aggggtgtta tatttgaatc acggtaccat tgttaaagta tcaggaccct tggtggtggc
     2821 agaaggcatg aggcatgcca gcatgtttga cgttgtaagg gttggaaaag caaatctaat
     2881 aggtgaggtt atagagatgc atggggacag ggcatccata caagtgtatg aggagacgga
     2941 aggtcttggc cctggagatg tggttgtatc tacaaatctt cccatgagtg tcgagttggg
     3001 tcctggtttg ataggaagca tattcgatgg aatacaaaga cctcttgatg agataatgaa
     3061 aatatcagga cacaacctta caagaggtgt catggtgcct tcattaaaca gggagaggaa
     3121 atggaatttc gtcaagtgcg tgaatgaagg agcgtatgta gaagcgggag atgttctagg
     3181 caccgttact gaaacggaag ttgttgtgca taaaatcatg gtgccatatg gagtttcagg
     3241 taccattttg aagatagagg aaggttcttt tacagtcacc gatactatag cagttgttga
     3301 aactgaaaaa gggaaagtgg agcttgccat gatgcagaca tggccggtaa gaaaggaaag
     3361 gccgtatcaa aagaaactgc cccctgagat gcccatggtg acaggacaga gggttattga
     3421 ctgcctcttc cctgtggcaa agggaggcgt ggctgcaatt cctggtcctt ttggcagtgg
     3481 aaagacaata acgcagcacc agttggcaaa atgggcggaa gccgacattg ttgtgtatat
     3541 tggatgtgga gaacgaggca atgagatgac ggatgttcta atggagtttc cgaaactggt
     3601 agaccccagg actggaaaat ccattatgga aagaacagtt ttgattgcaa atacttccga
     3661 tatgcccgtt gctgcaaggg aagcatctat ttatacaggt ataacgattg cagaatactt
     3721 tagagatatg ggatattccg tagcgctgat ggcggattcc acttcaagat gggctgaagc
     3781 tttgcgagaa atgtcgggca gacttgagga gatgcctgga gaggaaggct atccggcata
     3841 cctgggaagc cgtcttgccc agttctatga aagagcaggt cgtatcgtct cattgggttc
     3901 tgaaaaaagg gaaggtaccc ttacggccnn nnnnnnnnag cagtttcgcc accgggcgga
     3961 gacttttcag agcctgtaac ccaggcaacc cttaggattg taaaagtatt ctgggggctg
     4021 gatgcaaatc ttgcatacaa gagacatttt cctgcaataa attatctgac aagttattcg
     4081 ctttacgtag atactttgga aaaatggttt gatgagaata tttcaccact ttggtgcaag
     4141 cttagaataa ggatgcttca gactcttcag gatgaagcgg agctggagga gattgtaaag
     4201 ctggtaggta tggattcctt gtcgccccag gacagactca agatggaagt ggcaagatcc
     4261 ataagggaag actttctaca tcagcttgcc ttccatgaag ttgacacata cacaagtctt
     4321 gaaaaacagt ttttgcttat gagactcatt tttgaatact atgatgaggc tgtcattctc
     4381 cttaaagacg gaggaaatat tgaggaaata gttaatattc ctgtgaggga agccattgga
     4441 aggttcaagt atgttaaaga agaagagata catgcaagat acgctgatat aagtgagaag
     4501 attaggaatg agcttaaggc tttggctggc aaggaggaga tataatgcct gttgagtata
     4561 agaccataga agaagttgca ggaccgctaa tgcttgttag ggacgtggaa aatgttacat
     4621 acaatgaaat gggcgagatt gagctggcaa atggagagat aagacgttgc agagtgctgg
     4681 aagtggacgg aagcaatgcg cttgttcagc tgttcgaaag ctctattggt attaacctct
     4741 ccaacagcaa ggtgcgtttt catggcaagc ccatggaatt tggagtttct gaagacatat
     4801 taggcagagt ttttgacggt cttggtaatc caatagatgg aggtccacct attattccgg
     4861 aaaaaagaat gaatattaac ggacttccca tgaaccctgt ggcaaggaac tacccttcag
     4921 agttcatcca gacaggagta tctgcgatag acggccttaa caccctggta agaggacaaa
     4981 aactgcccat attctcaggc agcggtcttc ctcattcaaa gctggctgca cagattgcaa
     5041 ggcaggcaaa gacaagagga gcagaggagc cctttgctgt tgtttttgct gcaattggaa
     5101 ttacatttga agaagccaac ttttttatag aaagctttaa ggaatctggg gctattgaca
     5161 ggactgtatt gtttgtaaac cttgcaaacg atccagctat agagaggata tccactccaa
     5221 gacttgctct tactgccagt gaatatcttg cttttgaaaa aggaatgcat gtccttgtaa
     5281 ttcttaccga tattaccaac tatgcggatg cacttagaga agtgtctgcc gcaaagaagg
     5341 aaataccggg caggagaggt taccccggtt acatgtatac agaccttgcc accctttatg
     5401 aaagagccgg aagacagaag tcaaaaccag gttccattac aatgatacct attcttacaa
     5461 tgcccgagga tgacaaaact catcccatac cagaccttac aggctacatc acagaaggtc
     5521 agataatact aagcagggaa ctgtacagaa agaacattct tcctcccgtg gatgttctgc
     5581 catcactttc aaggcttaaa gacaagggta ttggtgaagg caagacaagg cacgaccatt
     5641 ccgatacaat gaaccagctc tttgcagctt acgcaagagg taaagatgca agggaactta
     5701 tgatggtctt aggtgagtct gctcttactg atatggataa actgtatgcg gaatttgcag
     5761 aagcctttga aagggaatac gtatcccagg ggtttgaaac agatagaaca attgaagaga
     5821 ctcttgattt aggttggaag cttttaagga tgcttcccag aaccgagctt acaagaatca
     5881 gggaagagaa cctggaaaag tattattgaa cgcagggtgg tttgaatggc gatactggca
     5941 gtaaatccta caaggatgga actttcaaaa cttaaaaaga aactaactgt tgcaaggcgc
     6001 gggcacaagc ttctgaaaga caaatgcgac gagcttatgc gtcagtttct tctgcttgtc
     6061 aaggagaaca aagctctgag aatggaagtg gaagaaggca taaggaatgc ccataaaagc
     6121 atgattgttg caagaagtgt tatgaaggaa gaggagattt caaatgcact catggtatcc
     6181 aagcaagaaa tacaagtaga gaaaaaggaa agaagcatca tgagtgttgc aattcctgtt
     6241 tttgaaacca gcttcaaaac tgaaaatgag caggata
//
LOCUS       DAONVY010000062         6244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9389_length_6244_cov_3.486508, whole genome shotgun sequence.
ACCESSION   DAONVY010000062 DAONVY010000000
VERSION     DAONVY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9389_length_6244_cov_3.486508"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLI11_02730"
     CDS             <1..607
                     /locus_tag="PLI11_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51811.1"
                     /translation="IDPFDGDLYQWCKFKIKNNSNAEFFQFHFDAGSGIKGDTNTLVP
                     ISKDDEEFKEYVFNMKDRNLATAEWSQDKRYPFTLEESLWTDTIRGIRLDCMFTDFPG
                     GQILTGSEFDLEYIAFFTSEEEALNFTPDRTRTPTPEPTKTPEPTKATTSAPTSTATS
                     TPAKQDEKKDESFPIHYVLIPAAVVVAAVVVVVILVKKKKK"
     gene            680..1351
                     /locus_tag="PLI11_02735"
     CDS             680..1351
                     /locus_tag="PLI11_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPZ51812.1"
                     /translation="MKETKKRKLINNFYTLGEEIFSSITHGIGALLGIAALVLSIVFA
                     ALYGDAMGVVSASIYGATMVIMYTMSTLYHALTHKIAKKVFRIFDHCSIFLLIAGTYT
                     PYTLVTLRGPIGWVLFGIVWATAILGIVFNSINMEKFKKFSMASYILSGWVIIFAMKP
                     LVENLKMGGIILLIAGGVLYTLGILFYAIKIPYAHPIWHLFVLAGSICHFFSILLYVL
                     PTDKL"
     gene            1377..1907
                     /locus_tag="PLI11_02740"
     CDS             1377..1907
                     /locus_tag="PLI11_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51813.1"
                     /translation="MNDRNNKQMLVTASVLILLNILIIKIFGLDNFSFERVNPVFAYI
                     FSFLYVIVHIIICYVLRYKRKQGILKGIFLYQFIGAFSFVILFVFLLMGIEVGFLRTI
                     FNWWSLPLEPISLILSLYGFFAKGIFRGIVYLAHTALTGNAYVQIKKDIAFENKIKEK
                     KAMEEESRLRQEQSGS"
     gene            complement(1896..3338)
                     /locus_tag="PLI11_02745"
     CDS             complement(1896..3338)
                     /locus_tag="PLI11_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPZ51814.1"
                     /translation="MKRCDVAIITNNSSGINLAINLDRKEYHVALYDVDIVIDGKVTT
                     VKDQNTRLISTDIQQIYDLSTLLLILDDPKIVFIIGDNNVFAEQMLSVLKERLDAGDI
                     VIDCCNTYYEDTANRTRSFEQMGILYLGTGLVGDTLKQPCLMPGGSYSAYMASIDILQ
                     DLSDKHCTYMGPDGAGQFVKMVHDAIEWSILQLISEHIGVLRAGLDLEYEELHYLLCD
                     WNSKETLNSYLLDVVADIIGRYDFESRRPLIDIASDVVGFDLSSSWIGKSAMLLAVPI
                     PTLTEAINVRFLTYMKGERQAISNFLPSFSIKVSGNESQSYISRAESSLYICLSLTYV
                     QGFDLIAKASDMYGWQMNIGDVAQAFSNGSYIKSRMLNRIVNLLKKNRSYKNLLMDSD
                     YLRVSTSHFPSLRRMVATAAEGGYPMPCTFACLSYLDTVAQSKLPTGIIQLASDYING
                     SGYERIDKDGVFYADWHDPNSGTRSIKSRT"
     gene            complement(3345..5069)
                     /locus_tag="PLI11_02750"
     CDS             complement(3345..5069)
                     /locus_tag="PLI11_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPZ51815.1"
                     /translation="MKNIPLYEVDQISTLKDMIINSEKKFGDKPAFLKKVPGIDNYVP
                     VTYKQYKKDVDALGTKLIDLGYKDEKIVIIGENRYEWSCSYLAVVNGVGVVVPLDKEL
                     PENEIELSVNRAEASLVIFSPKLIDKIDNLYSKLPTVKEYIVMDDESSDDRFVAFGKL
                     LKDGHELLDKGDDRFTSSEIDPEEMKILLFTSGTTDVAKAVMLSHRNICENLMAMCSM
                     TYIDENDVFLSILPIHHTYECTCGFLCPLYRGSTVAYCEGLRHIAKNMAEAKVTIVLG
                     VPLVFESMYKKIWDGIRKAKQEKKVRFAIRLNNFLKILGMDKSKNMFEKIHASFGGHL
                     RLLISGAAAIDPEVAKGFRDLGILFIQGYGLTECAPIVALNRNVDFKDAAAGLPMPGV
                     DIVIDNPDENGIGEIIVKGPNVMLGYYKDEAATKQVIDSKGYFHTGDLGYMDKEKFVY
                     ITGRKKNVIVTKNGKNIFPEEIEYLLLKSPYIQEVLVYASDEAGDTIVKANIFPNFEK
                     IEEDFKNGVISSDNVEAIIQSEIKNVNKQLVNYKSIRGFNIRETEFSKTTTRKIKRYV
                     EENKAEND"
     gene            complement(5073..5888)
                     /locus_tag="PLI11_02755"
     CDS             complement(5073..5888)
                     /locus_tag="PLI11_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPZ51816.1"
                     /translation="MKIAFSTLGCPDWSFSEILSTAKDFGYNGIEIRGLKNEIFAPAA
                     REFKEDRIETTRQSLKELGLEIACFSSASYLFDKKNTEDAIKEGCSYIDLANKMGVEY
                     VRVLGDRDPHPSKDIDDDFVRENLQYLVDYGNDKGVVPLIETNGVFADSKRLVKLVDK
                     IDGNVGILWDIHHPCRFFRENPQDTFDRIEPYLRYVHFKDSVFESGQVRYKMPGYGDL
                     PVRQAINLLLDNDFEGYVSLEWVKRWYYDLEEPGVVFMHFAHYIRKLERKKGK"
     gene            complement(5913..>6244)
                     /locus_tag="PLI11_02760"
     CDS             complement(5913..>6244)
                     /locus_tag="PLI11_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPZ51817.1"
                     /translation="FLTEGSKSNLFFVKGDTVYTAPPSQVLVGTMRKYVLKACENNMI
                     PIKLEAVHHDNISEYDAAFLTGTSIKVLPIVKIDEVEYKSSKNEIVLKLMKGLEQIIK
                     EYYNSNN"
BASE COUNT         1919 a         1317 c         1022 g         1986 t
ORIGIN      
        1 aattgatcct tttgacggcg acctttatca gtggtgcaaa tttaagatta aaaacaacag
       61 caacgctgag tttttccagt tccactttga tgcaggatca ggtataaaag gagatacaaa
      121 cacactagtg ccaatatcta aagacgacga ggaattcaaa gaatacgtat tcaatatgaa
      181 agatagaaac ctagcaactg ccgaatggtc acaagacaaa aggtatcctt ttacgcttga
      241 agaatcatta tggacagata ctatcagagg tataagattg gactgcatgt ttactgactt
      301 cccaggcgga caaatactaa ccggttccga gtttgacctt gaatacatag ccttcttcac
      361 atcggaagaa gaagcactaa acttcacacc ggatcgtaca agaacaccaa ctccggaacc
      421 tacaaaaaca cctgagccaa ccaaagcaac aacttctgcc ccaacatcta cagctaccag
      481 cactcctgcc aaacaggatg aaaagaagga cgaatccttc cctattcatt atgtccttat
      541 accagcagcg gtagttgttg cagctgttgt tgtagttgta atattggtta agaaaaagaa
      601 gaaataacgt ttaaacttga aaaaacgctc caataatgat atactagagt ttgaatatta
      661 aatttgaggt tatttactaa tgaaggaaac taaaaaaagg aaactaataa acaattttta
      721 caccttggga gaagagatat tcagcagtat aacacatgga attggagcat tgttaggtat
      781 tgctgcatta gttttaagca tagtatttgc ggcgttatat ggagacgcaa tgggtgttgt
      841 aagcgcatcg atatatggtg cgacaatggt gataatgtat actatgtcca ctttgtatca
      901 tgcgcttaca cataaaatcg caaagaaggt cttcagaata ttcgatcact gctctatttt
      961 cctccttatt gccggcacct atacgcccta taccttggtt acccttagag gaccaatcgg
     1021 ctgggtactt tttggaattg tatgggcaac cgcaattctt ggaattgttt tcaactctat
     1081 caatatggag aagtttaaaa aattttcaat ggcaagctat atattatctg gatgggtaat
     1141 aatttttgcc atgaaacccc ttgttgaaaa ccttaagatg ggtggaataa tactgcttat
     1201 tgcaggagga gtactttata ctttgggtat tctattttat gctatcaaga taccttatgc
     1261 acatccaatc tggcatttgt ttgtattggc aggcagcata tgccacttct tctctattct
     1321 actgtatgta ctgccaaccg acaaattatg atttattaag gagtagtcta agtagaatga
     1381 atgatagaaa caacaaacag atgcttgtaa cagcatctgt attgattctg ttgaatatat
     1441 tgattataaa aatatttggt ttggacaatt tctcatttga aagagtaaac cctgtttttg
     1501 catacatttt ttcattttta tatgtcatag ttcatatcat catctgctac gttttaagat
     1561 acaaaagaaa acagggaata ttaaaaggaa tcttcctata ccagttcata ggagctttta
     1621 gcttcgttat actttttgtt tttctactta tgggcattga agttggtttt ttaagaacaa
     1681 tatttaactg gtggtctcta ccactagaac ccatatcact aatcctctcg ttatacggct
     1741 tctttgccaa aggaatattc agaggaatag tttacctagc acataccgcc ttgaccggaa
     1801 acgcatatgt gcaaataaag aaagatattg cttttgaaaa caagataaag gagaaaaaag
     1861 caatggaaga agaatcaaga ctaagacagg aacagtcagg ttcttgattt tatacttctt
     1921 gttccagaat tggggtcatg ccaatccgca tagaaaaccc catctttatc gattctttca
     1981 tacccgcttc cattaatata gtcgctggca agttgaataa tgcccgttgg aagttttgat
     2041 tgtgctactg tgtcaaggta tgaaagacat gcaaaagtgc atggcatcgg ataccctcct
     2101 tcagcagcag ttgctaccat tcttcttaac gaaggaaaat gagatgttga cacacgcaaa
     2161 taatcggagt ccattaaaag atttttataa cttctgtttt tctttaaaag attcactatt
     2221 ctgttaagca ttcttgattt gatatacgaa ccattggaaa atgcttgagc aacgtctcca
     2281 atgttcattt gccacccata catatcagag gcttttgcaa tcaaatcaaa accttgaaca
     2341 tatgtcaaac ttaaacatat gtacaaggag ctttccgctc ttgagatata gctttgactt
     2401 tcatttccag atacttttat tgaaaacgaa ggcagaaaat ttgatattgc ctgcctctcg
     2461 cctttcatgt atgtaaggaa tctcacattt attgcttctg taagtgttgg aattggtacc
     2521 gccaaaagca tggcactctt accaatccag ctggacgaca gatcaaaacc aaccacatca
     2581 gaagcaatat cgataagagg tcgtcttgat tcgaagtcgt atcttccaat aatgtcagca
     2641 acaacatcga gcaaatagct gtttaaagtc tctttggaat tccagtcaca cagaaggtaa
     2701 tggagttcct catattccaa gtccagtcct gctctaagaa caccaatgtg ctcagatatt
     2761 agctgtaaaa tggaccattc tatagcatca tgaaccattt ttacaaactg gccggcgccg
     2821 tctgggccca tatatgtaca atgcttgtca gacaaatcct gaagaatgtc tatcgaagcc
     2881 atatacgcac tgtatgaacc tccaggcata aggcaaggtt gtttcaaagt atcgccaaca
     2941 aggccagtac ccagataaag aatccccatc tgctcaaagg acctggttct gtttgcagta
     3001 tcctcgtaat atgtattgca gcagtcaatt actatatctc ctgcatcaag tctttccttt
     3061 aaaactgaca gcatttgctc cgcaaacaca ttgttgtctc cgataataaa cacaattttc
     3121 ggatcatcca atattaaaag aagcgtactc aagtcataaa tctgctgtat gtcagtacta
     3181 atgagccttg tattctggtc ctttacagta gtaactttcc catcgattac tatatccaca
     3241 tcatacaagg caacatgata ctcttttcgg tccaaattaa tggccaaatt tataccactt
     3301 gaattattag taatgattgc tacatcacat cttttcatag cgctttaatc gttctccgct
     3361 ttattttctt caacatacct ctttatcttt ctagtagtgg tcttgctaaa ctctgtttcc
     3421 cttatattga aacctctaat cgatttgtag ttaaccaact gcttgttaac atttttaatc
     3481 tcactttgga ttattgcctc aacgttatca gatgatatta caccattttt gaagtcttcc
     3541 tcaatctttt caaaattggg gaatatattt gcctttacaa tggtatctcc cgcttcatct
     3601 gaggcataaa ccagcacttc ctgtatatat gggctcttta aaagaagata ttcaatttcc
     3661 tcagggaata tgttcttacc attctttgta acaattacat tcttctttct tccagtaata
     3721 taaacaaact tttctttatc catatagcca aggtcacctg tgtggaaata tcctttggag
     3781 tcaattacct gcttggtagc agcttcatcc ttgtaatatc cgagcatgac atttggacct
     3841 tttacaataa tttctcctat gccattttca tcaggattat ctataactat atcaacacca
     3901 ggcattggca aacctgctgc tgcgtccttg aaatccacat tcctgttaag cgcaacaatg
     3961 ggtgcacact cggtaagacc atagccttgt atgaaaagta tgcccaaatc cctgaaacct
     4021 ttggcaactt caggatcaat tgcagcagct ccactgatta aaagtctcaa atgaccgcca
     4081 aaactagcat gaattttttc aaacatattt ttagatttat ccataccaag aatcttcaag
     4141 aagttgttaa gtctaatagc aaacctgacc tttttctcct gttttgcctt ccttataccg
     4201 tcccaaattt tcttgtacat ggactcaaat accaatggca cgccgagaac aattgtaact
     4261 ttggcttcag ccatattctt cgcaatatgt ctgagtcctt cgcaataagc aacagtgctg
     4321 cctctgtaca aaggacaaag gaaaccacag gtgcactcat aagtatgatg tatgggcagt
     4381 attgaaagaa aaacgtcatt ttcatcaatg tatgtcatgg aacacatggc cataaggttt
     4441 tcacatatat tcctatgact aagcattaca gcctttgcaa catctgttgt acctgaagta
     4501 aacagaagaa tcttcatttc ttcaggatct atttcactac ttgtaaatct atcgtctccc
     4561 ttatcaagca gttcatggcc atccttcaac agtttaccaa aggcaacaaa tctgtcgtca
     4621 ctggattcgt catccatcac aatatattcc ttgacagttg gaagtttact atataaatta
     4681 tctatcttgt caattagttt tggtgaaaaa ataacaagac ttgcctctgc cctgttgact
     4741 gacagttcta tctcattttc cggaagttcc ttgtcaagag gaacaacaac tcctactcca
     4801 tttacaacag caagataaga acaagaccat tcgtacctat tttcaccaat aatcactatc
     4861 ttttcgtctt tgtatcccag atcaataagc ttggtgccta acgcatccac atctttcttg
     4921 tactgtttat atgttacagg tacataattg tcgattcctg ggaccttctt taaaaacgcc
     4981 ggtttgtctc cgaacttctt ttcggaatta attatcatgt cctttaatgt agaaatttga
     5041 tctacttcat atagcggtat attcttcata ttttacttac ctttctttct ttctagtttt
     5101 ctaatataat gtgcaaaatg catgaatact actcctggtt cctcaagatc ataataccat
     5161 cttttaaccc attccaaaga aacatagcct tcaaaatcat tatccaaaag gaggttgata
     5221 gcctgtctga caggaagatc accgtatccg ggcatcttgt atctcacctg accggattca
     5281 aatacggaat ctttgaaatg aacatatctc aaatacggtt caatcctatc aaaagtatct
     5341 tgaggattct ccctgaaaaa cctgcacgga tgatggatat cccatagaat acctacattt
     5401 ccatctattt tatcaaccag tttgacgagt cttttggaat cagcaaagac gccgttagtc
     5461 tcaattaaag ggactactcc tttgtcattt ccgtagtcaa caagatactg cagattttct
     5521 cttacaaagt catcatcaat gtctttggat ggatgcgggt ccctgtcacc aagaactctt
     5581 acgtattcaa cacccatctt gttcgccaaa tctatataac tacatccttc cttaatggca
     5641 tcttcagtat tcttcttatc aaaaagatag ctggcagaag aaaagcacgc aatttcgaga
     5701 ccaagttctt ttaaagactg cctagtagtt tcaattctgt cttctttaaa ctctctcgct
     5761 gctggtgcaa atatttcatt cttcaaaccc ctgatttcta taccattata accaaaatct
     5821 ttggctgtag acagtatctc cgaaaaagac caatcggggc atcccaaggt tgaaaaggca
     5881 attttcatgt tatctccccc actttccatt atttaattat tggaattgta atactcttta
     5941 ataatttgtt caagaccctt catcagtttt aaaacgattt cattttttga tgatttatat
     6001 tcaacttcgt ctattttcac aattggcaaa acttttattg acgtacctgt caaaaaagca
     6061 gcatcatact cactaatatt atcgtgatga accgcctcca acttgatagg aatcatattg
     6121 ttttcacatg ctttaagtac atactttctc attgttccaa ccaaaacctg actgggtggt
     6181 gcagtatata ctgtgtcacc cttgacaaaa aacagatttg acttgctgcc ttctgtcagg
     6241 aaac
//
LOCUS       DAONVY010000063         6239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9395_length_6239_cov_3.909120, whole genome shotgun sequence.
ACCESSION   DAONVY010000063 DAONVY010000000
VERSION     DAONVY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9395_length_6239_cov_3.909120"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLI11_02765"
     CDS             complement(<1..788)
                     /locus_tag="PLI11_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017553536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPZ51818.1"
                     /translation="MFSKILIANRGEIAVRIIRACKEMGIETVSVYSEADRDSLHVSM
                     ADESYCIGPAPSKDSYLNMAAILSAAIYSGAQAIHPGYGFLAENAKFAELCEKCNIVF
                     IGPRPEVISLMGDKDMARKTMQKAGVPIIPGCELVKTEKEAKEEAEKIGYPLLIKAKA
                     GGGGRGIRKVDGEKELVNAFYQATSEAKAAFGDGGVYMEKFLSPVRHIEVQVIADNYG
                     NAVALGERDCSIQRRNQKLIEESPSPAVASELREKLCKDSLKAA"
     gene            complement(804..1214)
                     /gene="fabZ"
                     /locus_tag="PLI11_02770"
     CDS             complement(804..1214)
                     /gene="fabZ"
                     /locus_tag="PLI11_02770"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPZ51819.1"
                     /translation="MNREEIKQIIPHREPMLLVDEAELIDENSAKGKYTVKGDEWFLQ
                     GHFPGNPVVPGVILCEIMAQTCSVILADKVKGKTPYFTGLNNVKFRNQVKPGDVLEIE
                     GRITRMRGNFCFGEATGSVDGKVCVSAEFSFAII"
     gene            complement(1226..1660)
                     /gene="accB"
                     /locus_tag="PLI11_02775"
     CDS             complement(1226..1660)
                     /gene="accB"
                     /locus_tag="PLI11_02775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPZ51820.1"
                     /translation="MMNINDIKCLAEVMKKNGLSKLEFEDGQIKLHLERNVEYEVVKS
                     SVEVKSEETFEEEESISVDQNISEVKAPLVGVFYAAKAPGEPPYVEVGDKVGKGDTLC
                     LIEAMKIMNEIPSDVDGTIEEICVTNGQIVEFGQVLFRIRKN"
     gene            complement(1647..2897)
                     /gene="fabF"
                     /locus_tag="PLI11_02780"
     CDS             complement(1647..2897)
                     /gene="fabF"
                     /locus_tag="PLI11_02780"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPZ51821.1"
                     /translation="MRRVVVTGLGVVSPVGSKIDTFWSNLVNGKNGIDYITYFDTTNY
                     KVKIAAEVKGFNASDYFERSEARKMDLFSQYAVAAADQAVRDSGLEADKVDGTRFGVY
                     VGSGIGGIKTFYEETLKLVQNGPRRVSPFFVPMMISNMASGLIAIRYGAKGPCLPVVT
                     ACATGTHAIGEAYRNILHGYSDIIIAGGSEAAITELAIAGFINCMALSTTNDPNYSSI
                     PFDLNRNGFVMGEGAGMLILEEYEHAVKRGAKIYAEVKGYANTCDAYHITAPDPETEM
                     PALAIKSAAEQCGIGKNDLSKVYVNAHGTGTPLNDKCETMAFKKAFGEGAYELGISST
                     KSMTGHMLGAAGAVEAIVSVLALRDGVIPPTINLKAKDPDCDLDYTPNQSVRKGLKYA
                     LSTSLGFGGHNACIAFERMEDDEH"
     gene            complement(2917..3657)
                     /gene="fabG"
                     /locus_tag="PLI11_02785"
     CDS             complement(2917..3657)
                     /gene="fabG"
                     /locus_tag="PLI11_02785"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPZ51822.1"
                     /translation="MLTGKVCVITGGTRGIGYRIAERMAENGADIAIIARNFKENFVE
                     SFKEGLERDFRIRSYKCDVSNFEETKETMDSIIKDFGQIDVLVNNAGIVKDKLILSMK
                     EDDFDDVMNVNLKGAFNTIRHTFSHFAKRRAGSIINISSVSGLLGTAGQANYASSKAG
                     LIGLTKTVARELAQRGVTCNAIAPGFIETDMTNVLSDKLKETYIEQIPMKKFGKTDDI
                     ANLAVFLASDSARYITGEVIRVDGGLCM"
     gene            complement(3651..4562)
                     /gene="fabD"
                     /locus_tag="PLI11_02790"
     CDS             complement(3651..4562)
                     /gene="fabD"
                     /locus_tag="PLI11_02790"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPZ51823.1"
                     /translation="MKAFVFSGQGAQVPGMGKKLYEKSEKARKIFDSVEKVIPGIKRL
                     CFESSRDELNETVNTQPCVFTVDLAAAYAVKATGIEPVAVAGFSLGEIAAIAYAEVLE
                     FDDACRLVRKRAELMHKDALERKGSMAAVLGMEKEEVLAGIKGYESIEAVNFNCPGQI
                     VVSGKEDEIDQYMADIKKFGKKARKLSVSGAFHSSFMNNASSKMGEYLKTIKTKKPNL
                     DVYSNLTAQPYPDDEEGIKDNIKKQINNPVRWEEIIRNMINSGIDTFIEVGEGKVLCG
                     LIERIDNNVKVFHYTDILNKDGGNSIC"
     gene            complement(4559..5506)
                     /gene="fabK"
                     /locus_tag="PLI11_02795"
     CDS             complement(4559..5506)
                     /gene="fabK"
                     /locus_tag="PLI11_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HPZ51824.1"
                     /translation="MIKTPLCELLNIKYPIIQGGMAWVADANLASAVSNAGGLGLIAA
                     MNMPAEALRQEIRKMRKLTDKPFGVNIMLMSPYAEEVAKVVCEEKVEVVTTGAGNPSK
                     YMEMWNENGIKVIPVVASTAHAKKVIRGGAVAVIAEGQEAGGHIGELTTMVLIPQICD
                     AVDVPVIAAGGIADGRTFAAAMMLGAVGVQVGTRFLVAKECNISQAYKDMVVKAKDID
                     TIVTGKRLGHPVRCLKSPFSRSYAKCEYDSNVTNEELEKLGSGALRLAAVDGDTKNGC
                     FMAGQSAALVREEQTAKEIIEDMVFGAEKVLKTSGRWIV"
     gene            complement(5507..>6239)
                     /locus_tag="PLI11_02800"
     CDS             complement(5507..>6239)
                     /locus_tag="PLI11_02800"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HPZ51825.1"
                     /translation="KLDLIICATLSAEYQSPSLACLVQKELGAVCPAVDINAACTGFI
                     YALDMADSYFKSGKCKKVLVVAFDALSKFVNWKDRSTCVLFGDGCGAVVLGEGKGLLH
                     SQVHAQGDMSSLLIGDLGDGKPYIHMIGKEIYKFAVSTGSTEIPDAIEKAGLKQSDVD
                     HVILHQANRRIMEAVAERLEIEKDKLAINIEEYGNTCSASIPLTLDKMNRNNLLKDGQ
                     IIVMTAFGGGLTSGTCVIEWNKGEK"
BASE COUNT         1540 a         1659 c         1013 g         2027 t
ORIGIN      
        1 cttgcggcct tcaggctgtc tttacacagt ttttctctta attcactggc tacagcagga
       61 gatgggcttt cttcaattag tttctggttt cttctctgga tggaacaatc cctctctcca
      121 agagccactg catttccgta attatccgca attacctgta cctcaatatg ccttacagga
      181 gacaggaatt tttccatgta cacgcctccg tcgccaaaag cagctttagc ctcgctggtt
      241 gcctgataaa atgcgttaac aagctccttt tcaccgtcaa ccttccttat acctcttccg
      301 cctcctccgg cttttgcctt tatcaataga ggatacccta ttttctcagc ttcttccttt
      361 gcttctttct ctgttttaac aagttcacaa cctggaatta ttggaacgcc agccttctgc
      421 atggtctttc ttgccatatc cttatcgccc atcagggata tgacctcagg acgaggacct
      481 ataaatacta tgttgcactt ctcgcacaac tcagcaaact ttgcattttc cgcaagaaaa
      541 ccgtaaccag gatgtattgc ctgggcaccg gaataaatgg cagcactcaa aatagctgcc
      601 atgttgagat agctgtcttt ggaaggtgca ggacctatgc aatagctctc atccgccata
      661 ctcacatgaa gtgaatctct gtcagcctcg gagtatacac tgacggtctc tattcccatc
      721 tctttgcaag cccttattat ccttactgca atttctcctc tgtttgcaat cagaatcttt
      781 gaaaacatat gccaacctct ttactaaata attgcgaaag aaaactcagc tgaaacacaa
      841 acctttccgt caacactgcc tgtcgcttct ccaaagcaga aatttcctct cattcttgta
      901 attcttcctt caatctctaa aacatctcca ggttttactt gattcctaaa cttaacattg
      961 ttcagtccag taaaatacgg tgtttttccc ttgaccttgt cagcaagaat cacactgcag
     1021 gtctgtgcca ttatttcaca tagtattact cccggaacta caggatttcc aggaaagtgt
     1081 ccctgaagaa accactcgtc accctttaca gtgtattttc cttttgctga attctcgtca
     1141 atcaattccg cttcatccac caaaagcatg ggctctctgt gaggtataat ttgttttatt
     1201 tcttccctgt tcataactct tctccttaat tctttctgat tctgaaaagc acctggccaa
     1261 actcaactat ctgaccatta gtcacgcaaa tttcttcaat tgtgccatcc acatctgatg
     1321 gaatctcatt cattatcttc attgcttcaa taaggcacaa cgtatcccct ttgccaacct
     1381 tgtcgccaac ttcaacgtac ggaggctcgc ccggtgcttt tgcagcataa aatacaccta
     1441 ccaaaggagc ctttacttct gaaatgtttt gatctacgga aatactctcc tcttcctcga
     1501 aagtctcttc actcttaact tccacactgc tttttactac ctcgtattca acattccttt
     1561 caaggtggag cttgatttgt ccgtcttcaa actcaagctt tgacaaacca tttttcttca
     1621 taacttcagc cagacattta atatcattaa tgttcatcat cttccatcct ttcaaacgct
     1681 atacatgcat tgtgaccgcc aaaaccaagg gatgtggaca aggcatattt caatcccttc
     1741 ctgaccgact ggtttggcgt atagtcaagg tcgcagtcag ggtcttttgc cttcagattt
     1801 attgtcgggg gtatgacgcc gtcccttaat gccagtacgg aaactattgc ctccactgcg
     1861 ccagcagctc ctagcatgtg tcccgtcatg gactttgtag agcttatacc aagctcatac
     1921 gctccttctc caaacgcttt tttaaatgcc atggtttcac atttatcgtt caatggtgta
     1981 cccgtaccat gggcattcac ataaaccttt gacaagtcat tcttgcctat accgcactgc
     2041 tccgcagcac tttttatagc gagtgcaggc atttccgttt cgggatctgg agcggttatg
     2101 tggtaggcgt cgcatgtatt tgcatatcct tttacctccg cataaatttt ggctcctctc
     2161 ttaactgcat gctcatactc ttcaagaatc agcattccag caccctctcc cattacaaaa
     2221 ccatttctgt tcaaatcaaa gggaatggag ctgtaattcg gatcattcgt tgtgctcagc
     2281 gccatgcagt ttatgaaacc tgcaattgcc agttcggtaa tcgccgcctc cgaaccccct
     2341 gcaataatta tgtcggaata accgtgaagg atgtttctat atgcctcacc tattgcatgg
     2401 gttcccgttg cacaggctgt aacaacagga agacagggcc cttttgcacc atacctgatt
     2461 gcaatcaagc cggaagccat gttggatatc atcataggca caaagaaggg cgatactctt
     2521 ctcggaccat tttgtacaag tttcaatgtc tcttcataaa aggtctttat gcctccaata
     2581 cccgaaccaa cgtacactcc gaatcttgta ccatcaacct tatcggcttc caatccgctg
     2641 tccctgacgg cttgatccgc tgctgctact gcatactgtg agaaaagatc catctttctt
     2701 gcttcgcttc tttcaaagta atcagatgcg ttaaaaccct ttacttctgc agcaatcttt
     2761 actttgtagt ttgttgtgtc gaaatatgtt atatagtcaa tgccgttttt gccgttgaca
     2821 aggttgctcc aaaatgtatc tattttgctt cccacaggag aaaccactcc caatccggtt
     2881 acaacaactc ttctcatata ttacttcctc ccttaattac atacacagac cgccgtcaac
     2941 cctgatgact tctcctgtaa tgtatcttgc agaatcagat gcaagaaata ctgcaagatt
     3001 tgcaatatca tcagtcttgc caaacttttt cataggtatc tgctcgatat atgtttcctt
     3061 tagtttgtcg gacaaaacgt ttgtcatgtc cgtttctata aagccaggtg ctattgcgtt
     3121 acatgttact cctctttgag ccagctctct ggcaacggtc ttcgtcaaac ctataaggcc
     3181 tgccttggag cttgcatagt ttgcctgccc tgctgttcca agcagaccgg atacgcttga
     3241 tatgtttatg atggaacctg ctcttctttt tgcaaagtgg gaaaatgtat gccttatggt
     3301 gttgaaagct cctttcaggt tgacattcat aacatcatca aaatcatctt ccttcatgga
     3361 aagtatcagc ttatctttta caatacctgc gttgttgact agcacatcta tttgtccaaa
     3421 atcctttatg atgctatcca ttgtttcctt tgtttcttca aaattggaaa catcacattt
     3481 gtatgatctt attctaaagt ctctttcaag gccttccttg aaactttcaa caaagttttc
     3541 cttgaaattc cttgctatta tggctatatc ggctccattc tcagccatcc tctcagcaat
     3601 cctgtaacca attcctcttg ttcctccggt aataacgcat acctttcccg tcaacatatt
     3661 gagtttcctc catccttgtt taaaatatcg gtgtagtgaa atactttcac attgttgtca
     3721 attctttcta tcaatccgca caagaccttt ccctctccaa cttcaataaa cgtatcaatc
     3781 ccgctattta tcatgtttct tattatctct tcccatctta ccggattgtt tatctgcttt
     3841 ttgatattat cctttatgcc ttcctcatca tcaggataag gctgggctgt caagttggaa
     3901 taaacgtcaa ggttcggttt ctttgtctta attgtcttta gatattcgcc catcttgctg
     3961 gaagcattgt tcatgaagct actgtgaaac gcaccgctta cgctcagttt tcttgccttt
     4021 ttgccaaact tcttaatatc cgccatgtac tggtctattt catcttcctt gcctgaaacg
     4081 acaatctgtc caggacagtt aaaattcaca gcttcaatac tttcataccc ttttatgcca
     4141 gctagaacct cttccttttc cattcccagc acagctgcca ttgaaccctt tctctctaga
     4201 gcatctttat gcataagttc agctcttttt cttaccaatc tgcaagcatc atcaaattca
     4261 agaacctctg cataagcaat agccgcaatt tctccaaggg agaatcctgc aacagccaca
     4321 ggttcaatcc ctgtcgcctt tacagcgtat gccgcagcca ggtccacggt aaatacacaa
     4381 ggctgcgtgt ttaccgtttc gttcagttca tcccttgaac tttcaaaaca cagtcttttt
     4441 attcctggta ttactttttc cacagagtca aatatttttc ttgccttttc agatttttca
     4501 tacaatttct ttcccattcc aggcacttgt gcaccctgtc cagaaaaaac aaatgccttc
     4561 atacaatcca cctgccggat gtctttaata ctttttcagc accaaatacc atatcttcaa
     4621 taatttcctt tgctgtttgt tcttccctaa ccaacgcagc gctctggcca gccataaagc
     4681 aaccgttttt tgtatctcca tcaactgctg caagcctcaa tgctccggaa cccagctttt
     4741 caagttcctc atttgttaca ttggaatcat actcacattt tgcataactt ctggaaaatg
     4801 gactcttcaa gcaccttact ggatgaccaa gtctttttcc tgttacgatg gtgtcgatat
     4861 cttttgcctt aacaaccatg tccttgtagg cttgacttat gttgcattct ttagcaacca
     4921 aaaatcttgt tcccacttga accccaaccg ctccaagcat catagctgct gcaaaggtcc
     4981 ttccatcagc aattcctcct gcagcaatta caggcacatc cacagcatca catatctggg
     5041 gaataaggac catggtggta agttcgccta tatgacctcc tgcttcctga ccttctgcaa
     5101 ttacagccac agcaccacct cttataacct tctttgcatg agccgtagat gccacaacag
     5161 gtatgacttt tattccattc tcattccaca tttccatata tttcgatggg ttacctgcgc
     5221 cggtagttac aacctcgacc ttttcctcgc acacaacctt tgccacttct tcagcatacg
     5281 gactcataag cattatattt accccaaaag gcttgtcggt aagttttctc atcttccgta
     5341 tttcctgcct caaagcctct gcaggcatgt tcatggcagc tatcaatcca aggccgcctg
     5401 cattgcttac tgcagatgca agatttgcat ccgcaaccca tgccattcct ccctgaataa
     5461 ttggatactt gatatttaat aattcacata acggtgtctt aatcacttac ttttccccct
     5521 tgttccactc aattacacat gttcctgatg tcaatcctcc accaaaggcg gtcattacaa
     5581 ttatctgtcc atctttcaaa agattgtttc tgttcatttt gtctagagtc agaggtatgc
     5641 tcgcagaaca cgtatttcca tattcctcta tatttattgc aagcttatct ttttcaattt
     5701 caagtctttc ggcaacagct tccattatgc gtctgtttgc ttggtgaagt attacatgat
     5761 ccacatccga ctgtttgagt cctgcctttt ctatggcatc tggaatttca gtgctgcctg
     5821 tgctgactgc aaacttgtat atttccttgc caatcatgtg gatatatggt ttgccgtctc
     5881 ccaggtcccc tatcaataaa gagctcatgt ctccttgggc atgtacctgg gagtgaagaa
     5941 ggcctttccc ttcacccagc accactgctc cgcatccgtc cccaaacaaa acacaagtgg
     6001 atctgtcttt ccagttaaca aatttggaca atgcatcaaa cgctactacc aacactttct
     6061 tgcatttgcc gcttttgaaa tagctgtctg ccatgtccag tgcataaatg aatcctgtgc
     6121 atgcggcatt aatgtcaact gcaggacaga ctgcccctaa ttccttttga acaagacatg
     6181 caagagaagg ggactgatat tcagcactga gtgttgcaca tattatcaaa tccaacttc
//
LOCUS       DAONVY010000064         6198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9480_length_6198_cov_3.489500, whole genome shotgun sequence.
ACCESSION   DAONVY010000064 DAONVY010000000
VERSION     DAONVY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9480_length_6198_cov_3.489500"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1124
                     /gene="tsaD"
                     /locus_tag="PLI11_02805"
     CDS             102..1124
                     /gene="tsaD"
                     /locus_tag="PLI11_02805"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPZ51826.1"
                     /translation="MKDIYILGIETSCDETSASIVKNGREVLSNIISSQIDLHKEYGG
                     VVPEIASRKHVELIIPVINEAIKSSGIKKEMISAVGVTFGPGLIGALLVGLSAAKAMS
                     YALNVPLVGVHHIEGHIAANFIEHKSLEPPFISLVVSGGHSHIIECTDYGEFKIHGRT
                     RDDAVGEAYDKIARTLGLGYPGGPVIDKIAKEGSAVIQFPRVHFENLDFSFSGLKTAV
                     INYVHNEKMRGNELNITDVCASFQSAAVDILVENTIKAAKLTGLGKIVLAGGVASNSC
                     LRARMNEEASKEGMEVYIPSPILCTDNGAMVASAAYFAYIKGRKSKLNLNAMANVSLE
                     EIAKHA"
     gene            1173..1454
                     /locus_tag="PLI11_02810"
     CDS             1173..1454
                     /locus_tag="PLI11_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51827.1"
                     /translation="MNTIGGGFLCRIHGPVSWACLCKEFVRDETKIKGIKCKDCVFFK
                     RIESVDDTDNNGANNVWWGTCLKYLLREFDGNSRQTCSQFIEKAQKYIT"
     gene            1756..2247
                     /locus_tag="PLI11_02815"
     CDS             1756..2247
                     /locus_tag="PLI11_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HPZ51828.1"
                     /translation="MRIIRKRLIPNEEVDISQDEVLIRNSDHIITRWLPIKPRGDIGW
                     GISYLDLNGNRKISAFYGKNGKLMYWYCDIVDVTYLEYSDLYIIKDLLVDVKLVPGQP
                     PEILDLHELEMALKQGLINKEEYALAVRVSKATLELLKHEIPDKIKEILKYGPPVGFE
                     IET"
     gene            2287..2754
                     /locus_tag="PLI11_02820"
     CDS             2287..2754
                     /locus_tag="PLI11_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51829.1"
                     /translation="MKKNAEVKDKSKKVHGLSLIGIIACTVINIFVFILQVMGINFKI
                     YIPYSIHATHYFLQIGYSLSEKTGAYMKFMGGVAASMLLVGIFILLYFWSFKNAEAFL
                     YAFILYLLDTAFIFYVIFKFDDSYAFVDFVFHLCGLLALILAFSEEKHNKKYK"
     gene            2778..4313
                     /locus_tag="PLI11_02825"
     CDS             2778..4313
                     /locus_tag="PLI11_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPZ51830.1"
                     /translation="MQKVEWKKLQNGSDIRGVALADGGNPVNLTTDVVKTIGSAFALW
                     LADKKSKEVKELSISIGHDSRLTAKSLSAAAIDGITDFGADVVNCNLCSTPAMFMTTV
                     TEGHEYDGAVMLTASHLPFDRNGMKFFTREGGLEKQDISKILELSEKIYNGEIKPDAN
                     KKGSVKDIDFVSVYAEGLVAKIRDATEEEKPLEGFKIIVDAGNGAGGFFADKVLEVLG
                     ADTNGSQFLDPDGTFPNHIPNPEDKEAMESICDAVIKKNADLGIIFDTDVDRAAVVDR
                     FGRDINRNRIIALISAIVLGERKGATIVTDSITSEGLKKFIEEDLGGVHHRFKRGYKN
                     VINEAIRLNEEGTYTPVAIETSGHCALMENYFLDDGAYLIAKILIQMAKLKKENRTID
                     QLIENLEEPKEEKEFRMKINLDDFKEYGQKVIDGLADYTKEQEGWIVEPVNHEGIRVK
                     CHKGNGDGWFLLRLSLHDPILPLNIESDSDGGVKVIAKSLLEYLEHYDKLDLSGIKSF
                     IEE"
     gene            4317..5015
                     /locus_tag="PLI11_02830"
     CDS             4317..5015
                     /locus_tag="PLI11_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51831.1"
                     /translation="MLRINELTKFYGQTCAVNNVSLEVPAGCISILLGPNGAGKSTVI
                     KSIAGLLRYSGRVEISGHENKSVEAKRYLGYVAEFPAMYELLTVREHLEFIARAYKLK
                     DWEDRAERLLEGFQLTDKADKLGSDLSKGMQQKLSIICGVLPQPKVCLFDEPLVGLDP
                     HAIKEFKNLVVQMRDEGCTILISTHMIDSVKEFWDSTYIMMDGKIAYHTTREIAEEMN
                     IDIEKIFFDITEKK"
     gene            5017..>6198
                     /locus_tag="PLI11_02835"
     CDS             5017..>6198
                     /locus_tag="PLI11_02835"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HPZ51832.1"
                     /translation="MGPCIYVIKRSCINWLKKLKEKPAVIVGIAVFFFIFIMPLLFQG
                     NSVDTTRAGGLNILYLIFFGLSLFFVGASLIAGLSTGTSFFTMSDVNLMFTSPISSNK
                     ILLYGIIKQMGRSIVVSVFMLFQIANLRANFTLSIFKSVYVIIIYMMLVFISQMVSLV
                     IYSFTNGNQRRKSAVKYTAMILLLIVIAYIVYGMINRGTEVIQEFFIFAVPVSGWLLG
                     MLVGVLSSNFVLLAISTGVIITFVLAVIGVMIKYKPDYYEDCLINSENAYHARQVMKG
                     DRSAQANIKKKIRYKNVKTIKFGGGWGASSFFYRHMVEIKRAERSVLFGTSSVLFLAL
                     GVGIGLVAKSDYDFVYAGFGIMCYVGLFLVGVTKLTQELSRHFVYLVPDSPFKKLLYA
                     SL"
BASE COUNT         1920 a         1011 c         1494 g         1773 t
ORIGIN      
        1 tatttttaca gtgataacat aaatattgaa gtagtaacgg ggtcaattct attgaccctg
       61 tttcgatatc tttttacatt ttatcttgag gagcaagcaa attgaaagac atatatattc
      121 ttggaataga gacaagctgt gatgaaacaa gtgcgtcaat cgtcaaaaac ggacgggagg
      181 tattgtcgaa tatcatctcg tcgcagattg atttgcacaa ggaatacggt ggtgtggtcc
      241 ctgaaatcgc ctcgcgaaaa catgtggagc ttatcatacc tgtcataaat gaggcaataa
      301 agagttcagg tattaaaaag gaaatgataa gcgcagtggg agttactttc gggcctgggc
      361 tgattggcgc tcttttggta gggctttctg ctgcaaaggc tatgtcatat gcattgaatg
      421 tgccactggt tggcgtccac catattgagg gacatattgc tgcaaatttc atagagcata
      481 aaagtttgga acctccgttt ataagtcttg tagtatctgg aggtcacagc catataatcg
      541 aatgtaccga ctatggagaa tttaaaattc atggaagaac cagggacgat gctgttggtg
      601 aggcctatga caagatcgcc agaacattgg ggctaggata tccgggaggg cctgtaattg
      661 acaagatagc caaagaagga agtgctgtca tacagtttcc cagagtacat tttgaaaacc
      721 ttgatttttc cttcagcggg ctaaagaccg cagttatcaa ctatgtgcat aatgagaaaa
      781 tgaggggaaa cgagcttaac atcactgacg tttgcgcatc cttccaaagc gcagcggtag
      841 acattcttgt tgagaatacc attaaagcgg caaagctgac aggccttggt aaaattgttc
      901 tggcaggtgg tgtagcatca aattcatgcc ttagggctag gatgaatgag gaagcttcaa
      961 aagagggtat ggaagtatat attccgtctc ccatactgtg tacggataat ggtgccatgg
     1021 tggcgtctgc tgcatacttt gcatacatta agggaaggaa gtcaaaactt aatttgaatg
     1081 caatggcaaa tgtgtctttg gaggaaatag caaagcatgc atgatatttg gaggaggcta
     1141 tgtataactg caacttctgc aagcataaaa gtgtgaatac aataggtggc ggatttttat
     1201 gtaggataca tggtcctgtg tcctgggcat gcttatgcaa ggaatttgtg agggatgaga
     1261 caaaaattaa aggaataaaa tgtaaggatt gtgttttctt caaacgaata gaaagcgttg
     1321 atgatacgga taataatgga gctaataatg tatggtgggg cacatgcctt aaatatttgc
     1381 ttagagaatt tgatggcaat agtagacaaa cctgttccca atttatcgaa aaagcacaga
     1441 aatatattac ataactggca gaaaaagcaa aatatgtact ggctatatag tgaattgtta
     1501 gtagttatac acattatcca cagagttatc cacaggttga ttgtttaaaa atatatagaa
     1561 atacaagctt tctgaaaagt tatccacatt ttgttgaaaa aaattatgca catgcatctc
     1621 ctaaaatttg tgtgaaaaat ggtatcaaaa attaatttta tagaatactt gattttttta
     1681 ttaaaatatg atttgtgagg tttgcatcac ggttacccgc atagtataat agcaaaaagc
     1741 aatggagtga aaactgtgcg gataattcga aagcggctaa tacctaacga ggaagtggac
     1801 atatcacaag atgaggttct tatcaggaat agcgaccata taatcaccag atggctgcca
     1861 ataaagccaa ggggagatat aggttggggg atatcctatc ttgaccttaa cggaaaccgt
     1921 aaaataagtg ctttttacgg gaagaatgga aagttaatgt actggtattg cgatatagta
     1981 gatgttacat atttagagta ttccgatttg tatataatta aggatttgct tgtggatgtt
     2041 aagctcgtac caggacagcc gcctgaaatc cttgacctgc atgaactgga gatggcttta
     2101 aaacaaggac tgattaacaa agaagaatat gctttggctg ttagggtgtc aaaagcaact
     2161 ttggaattgt taaaacatga aatacctgat aaaattaaag agatactaaa gtatggacca
     2221 ccggttgggt ttgaaataga aacctgaaca atatgtccat gttcattgta aaagaaaggg
     2281 caggttatga agaaaaacgc agaagtaaag gacaagtcta aaaaggtaca cggtttatca
     2341 cttattggaa ttatcgcttg cacggttatt aatatttttg tattcatact acaggtaatg
     2401 gggattaatt ttaagatata tataccctac agtattcatg ccacccatta ttttcttcaa
     2461 ataggatatt ctttaagcga gaaaaccggt gcatacatga agttcatggg aggcgttgcc
     2521 gcatccatgc tgctggtggg gatttttatc ctgctttatt tctggtcatt taaaaatgct
     2581 gaggctttcc tttatgcctt tatactgtac cttttggaca cggcttttat tttctatgtc
     2641 attttcaagt ttgatgacag ttatgccttt gtcgattttg tatttcattt gtgtggcctt
     2701 ctggcactta ttctggcatt cagtgaagaa aaacacaata aaaaatataa ataacaaaaa
     2761 tatatggagg aattgtaatg caaaaagttg aatggaagaa gttacaaaac ggaagcgaca
     2821 taagaggagt tgcgctggct gacggcggca atcccgttaa tcttaccacc gatgttgtaa
     2881 agacgattgg cagtgctttt gctctttggc tggcggataa gaagtcaaag gaggtaaagg
     2941 aactttcgat ttccatcgga catgattcca ggcttacagc aaaaagcctt agtgcagctg
     3001 caattgatgg aatcacagac tttggcgctg atgtggttaa ctgcaacctt tgttcaacac
     3061 ctgccatgtt catgacaact gtaacggaag gacatgaata tgacggtgct gtcatgctta
     3121 cagcaagcca tcttcctttt gacagaaatg gtatgaaatt ctttacaagg gaaggcggac
     3181 tagagaagca ggacatatca aagatacttg aattgtctga gaagatatat aatggtgaaa
     3241 tcaaacctga tgccaacaag aagggaagtg taaaggatat tgatttcgtt tcggtttacg
     3301 cagagggcct ggttgcaaag attagagatg ctacagaaga agaaaagccc cttgaaggtt
     3361 ttaagattat tgtggacgca ggtaacggag caggcggatt ctttgctgac aaggtgcttg
     3421 aagttttggg tgctgatacc aatggaagcc agttccttga tccggacgga acatttccca
     3481 accatattcc aaatcccgag gacaaggaag ctatggaatc catttgcgat gccgttataa
     3541 agaaaaacgc ggatttgggt ataatttttg atacggatgt ggacagggca gcggtcgttg
     3601 acaggtttgg aagggatata aacaggaata gaatcattgc tctgatttca gcgattgtgc
     3661 tgggtgaaag aaaaggtgca accattgtta ccgactccat aacctctgaa ggcctaaaaa
     3721 agtttataga agaggatttg ggaggagttc accaccgttt caagagagga tacaagaatg
     3781 ttatcaatga ggccataagg ctcaatgagg aaggcacata tacgcctgtg gctattgaga
     3841 catcggggca ctgtgctctt atggaaaact acttccttga tgatggtgca tatctgattg
     3901 ccaagatatt gattcaaatg gcaaagctca agaaggaaaa caggacgatt gaccagttaa
     3961 tagaaaacct tgaagagccc aaggaagaga aggaattcag gatgaaaata aatcttgatg
     4021 acttcaagga gtacggtcaa aaggttatag acggtcttgc agactataca aaagagcagg
     4081 aaggttggat tgttgagcct gtaaaccatg aaggcataag ggtcaagtgc cacaagggca
     4141 atggagatgg atggttcctg ctcagactat cactgcatga tccgatactg cctttgaata
     4201 tcgagtcgga ttccgatggc ggagtcaagg ttatagcaaa gagtttgctt gagtatcttg
     4261 agcattacga caagctggat ttgtcgggta ttaagagttt tatagaggag taaaacatgc
     4321 ttagaataaa tgaactgaca aaattctatg gacaaacatg tgctgttaac aatgtcagcc
     4381 ttgaggtacc tgcagggtgc attagcatcc tgctaggtcc taatggagct ggaaaatcca
     4441 ctgttatcaa gagcattgct gggctcttaa gatattctgg aagggtagaa ataagtggac
     4501 atgaaaacaa gtccgtggaa gctaaaagat accttggcta tgttgccgag tttccagcca
     4561 tgtacgagct gcttacagta agggagcacc tggagtttat agcgagagcg tacaagctca
     4621 aggattggga ggatagagcg gaaagactgc ttgaaggttt tcagctgacg gacaaagcgg
     4681 ataaactggg ttcggatttg tccaaaggta tgcagcagaa acttagcata atctgcggag
     4741 ttttgcctca gccgaaagta tgtctgtttg atgagccttt ggtggggctt gatcctcacg
     4801 caatcaagga gttcaagaat ctggttgttc agatgaggga cgaaggctgt acaattttaa
     4861 taagcacgca tatgattgac agcgtgaaag agttctggga cagcacctat attatgatgg
     4921 atggaaagat tgcatatcat accacaagag agatagcgga agaaatgaac attgatatcg
     4981 agaaaatctt ttttgatata acggagaaaa agtaaaatgg gtccatgcat ttatgttata
     5041 aaacgtagtt gtataaactg gttaaagaaa ctgaaggaaa agcctgcagt catagttggt
     5101 attgcggtct tctttttcat attcataatg cctcttttgt ttcaaggaaa tagtgttgat
     5161 actaccagag ccggcggcct caacatattg tacctgatat tcttcgggtt atcattattc
     5221 tttgtaggtg cttcattaat agcagggctt tccacaggca catccttctt cacgatgtcc
     5281 gatgtaaacc ttatgttcac atcccccata tcttcaaaca agatactgct ttatgggatt
     5341 ataaagcaaa tgggcaggtc tattgttgtg tccgtgttca tgctgtttca gattgcaaat
     5401 ttaagggcga attttacctt aagcatattt aaaagtgttt atgtgattat tatatacatg
     5461 atgcttgtat ttatatccca gatggtatct cttgttatct attcctttac gaatgggaat
     5521 cagaggagga aaagtgctgt caaatacaca gcgatgattc ttcttctgat tgttatagca
     5581 tacattgtgt atggtatgat caacagggga actgaggtaa tacaggaatt ttttatcttt
     5641 gctgttccag tctcaggatg gcttttggga atgcttgtcg gcgttttgag ctcaaatttc
     5701 gttttgctcg ccatatccac aggggtgatt attacgtttg tccttgcggt tatcggtgtc
     5761 atgattaagt acaaacccga ctattacgaa gactgcctga taaactcaga aaacgcatac
     5821 catgcaagac aggtaatgaa aggcgacagg tcggctcagg ctaacataaa gaagaagatc
     5881 agatacaaga atgtgaaaac cattaagttt ggtggtggat ggggggcatc cagtttcttc
     5941 tacaggcata tggtggagat aaaacgggct gagaggtcgg tattgtttgg cacgtcttct
     6001 gtactgttcc ttgctctggg agttggtata ggacttgttg caaagtcgga ttatgatttt
     6061 gtatatgcag gttttgggat aatgtgctac gttgggcttt ttctggtggg tgtaacaaaa
     6121 ctgacgcagg aactttcgag gcattttgta tatcttgtgc ctgatagccc tttcaaaaag
     6181 ctcctgtatg catccttg
//
LOCUS       DAONVY010000065         6130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9606_length_6130_cov_3.404280, whole genome shotgun sequence.
ACCESSION   DAONVY010000065 DAONVY010000000
VERSION     DAONVY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9606_length_6130_cov_3.404280"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="mraY"
                     /locus_tag="PLI11_02840"
     CDS             complement(<1..732)
                     /gene="mraY"
                     /locus_tag="PLI11_02840"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPZ51833.1"
                     /translation="MTVAIVVFLSTFALAALFGPIYIPILRRLKFGQTVRDDGPATHY
                     KKTGTPTIGGLVFLTPLVIVGLVLHYNGKAPEILPLLFVTIGFGFVGFLDDFIKIVKK
                     SKDGLLSYQKMSLLFIVSLIFTYYINNNADLGTVINIDLFGLHTEFDLGYAFIPFSIL
                     VLLSTTNTLNLTDGLDGLCAGTSFIILMFFALVSILVEKNNSVMYFSTALAGGVLGFL
                     LFNYNPARLFMGDTGALALGGAIGAC"
     gene            complement(729..2141)
                     /gene="murF"
                     /locus_tag="PLI11_02845"
     CDS             complement(729..2141)
                     /gene="murF"
                     /locus_tag="PLI11_02845"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPZ51834.1"
                     /translation="MLELTVKQILASTNGKLLNEKIIDEKITSIVTDSRRVTKGSLFV
                     CIEGEKFDGHDFCQDAFNSGAVACLVHKSIDGGNTNIIQVENTLTAYGDIASFYRNFY
                     EEKLGKPLIVVGVTGSVGKTSTKDYIFEVLSKKFRTLKSKLNENNEIGLPKTILNLVE
                     EEAIVVEMGMRGLNQIEYLSRIARPTHAVITNIGSNHLEFLGTKDNILKAKSEITIGM
                     AGDGVLVVNGDDEYLKQGDFGDVAVFKVGFDEDNYMRVIDYDASNSIYVFTDESGKEY
                     TVSLKLKGKHNVYNSLVAICIAKCLGVPVEEAVSACESYTGDSIRQNIFTLKNRNVTI
                     IDDAYNASYESMKASLEVLESFENRKVAVLGDMLELGEYSDEYHYKVGKLCVEKGVDI
                     VIACGKYSRNIVEAVMESMLDNMENKKVCYAYYTQDAKEAAQKLMLMLQEGDCVLVKG
                     SNAMKMGHVVEYLRESDVGI"
     gene            complement(2141..3619)
                     /locus_tag="PLI11_02850"
     CDS             complement(2141..3619)
                     /locus_tag="PLI11_02850"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPZ51835.1"
                     /translation="MKLNELVKGLDIEYIKGNAEREIEDIVVDSRLASKGSMFVCIKG
                     YVQDGHAFAQQAVNNGCGVLLVTDDVDVTGDVSIVKTKDTRKSLPYLADRLYNHPSGR
                     LKLIGVTGTKGKTSTTFYIKSVLEKAKYKTGLIGTMYNMIDDEILYTERTTPEANDLQ
                     RLLNEMANREINVCVMEVSSQGLKLGRVEFCTYSTAIFTNLSRDHIGETEHPDMEDYA
                     KSKAMLFGMSENSLINADSEYFDLMASNGKKVYSYGIEKDCDYRAVNIKKYADHVEYD
                     VLYGTKEGKTDKRHVYVSSPGTFSVYNSLAAIGTCHIEGIDMDTIVEGIRDVTVRGKA
                     EVVPTNRDFTVIIDYAHNPDSFINILTTVKEFAKRCVFLFGAGGDRNRPRKLMGETAG
                     KYADFTIITSDNPRTEDPEKIVKEIEEGIKPTGAEYICIVDRKEAIEYALRNALPGDV
                     IILAGKGHETYQIFRDKVIHFDEREVVRDILARMDEEDKRCS"
     gene            complement(3635..5881)
                     /locus_tag="PLI11_02855"
     CDS             complement(3635..5881)
                     /locus_tag="PLI11_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPZ51836.1"
                     /translation="MSFFNKNNKKSNETLMKIRAGNRSAALVVIFIIIIFGLLLRIAW
                     IQFVRGEEYQKRAYLQQNNGRIIPANRGTIFDRNGNILAISVNARQVSVNQQTIIKEG
                     EARGDVKAYQELVARALADILDLNYETVLEKVQSVGRYKEIARKIDVEVGEQVEGWIK
                     KNKIKGVHVDEDVRRYYPNNSIAAHILGFTGRDDQGLVCGVEVALDKMLAGKAGRIVT
                     EVDAKGVELPFDAERRIEPVNGYNATLTIDITIQYMVEQALQKAVIDQGVIGGAAGIV
                     MNPNNGDILAMASWPTFNLNDPYACPPDMDPSSWTGNSSESVQILSTTVWRNKALTDT
                     YEPGSTFKAITSAAVLEEGIANPSTIVNDAPLKLSGWTIRCWRKTYDHGNETFLEAIK
                     NSCNPVFAKLSLKLGIKKFYSYLDAFGFTRKTGLGLTGEADSIIHKDPKEIDMAVAAF
                     GQRLQITPIQLITAYSAIANGGNLLTPRIVKELTDEHGNVVMRYDTKVVRQVLSKETA
                     NTLLSMLEETVASGGGSNAYVTGYRVAGKTGTSQTPETDTTGRYVASFCGIAPADNPQ
                     IVILIVLDHPTVGVTSGGRQAAPVARELIEKILEYMEVERRYTELDKDKMLVKYKVPD
                     LSELTLSEAIDELKKNNLSYRIVGDYTDPDARIYEQMPRPGALVSKDSMVVIYTEKES
                     VKEKITVPDLMGLTLEEAYSALKERGLNMRASSLGTVVSQSIEPGKQVEIGSVVEIKL
                     LNEDIETA"
     gene            complement(5938..>6130)
                     /locus_tag="PLI11_02860"
     CDS             complement(5938..>6130)
                     /locus_tag="PLI11_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51837.1"
                     /translation="AEISQIAINELGMQKPQSYQIVRIKVVPTDQTEIHEMKFTKDEN
                     NTPWYKNVLDEIKAFLGIC"
BASE COUNT         1632 a         1529 c         1034 g         1935 t
ORIGIN      
        1 gcatgcacca attgctccac ccaatgcaag agctcctgta tcccccatga aaagccttgc
       61 aggattgtag ttgaaaagca aaaagcccaa aacgcctcca gccaaggcag tggaaaaata
      121 catgacagaa ttattctttt ccaccagaat tgacaccagt gcaaaaaaca taagaattat
      181 aaacgaggtg cctgcgcaaa ggccatcaag accatcggtc aaattaagcg tgtttgttgt
      241 actcagcagc acaaggattg aaaagggtat gaaggcatag cccaggtcaa actcggtatg
      301 taaaccgaac agatcaatgt tgattaccgt gccaagatcc gcattgttgt taatgtaata
      361 tgtgaaaatg agtgatacaa taaacaatag tgacatcttc tgatatgaca atagtccatc
      421 cttgctcttc ttcacaatct ttataaaatc atccaaaaat cccacaaatc caaagccaat
      481 ggtcacaaac aacaagggaa gaatttccgg agcttttccg ttgtaatgta aaaccaaccc
      541 tacaatcacc aagggagtca gaaaaacaag tccaccaata gtaggagtgc ccgttttctt
      601 gtaatgggtg gcgggaccgt catccctcac cgtctgacca aatttaagtc ttctcaatat
      661 tggtatatat attggtccaa atagagctgc tagagcaaat gtacttaaaa aaacaacaat
      721 agctactgtc atatccccac atcactttct ctcaaatatt caaccacatg ccccattttc
      781 atggcattgg agcctttaac caaaacacag tctccttcct gcagcataag catcagcttc
      841 tgggctgcct ctttagcatc ctgtgtataa tatgcgtaac aaaccttttt gttttccata
      901 ttgtccaaca tggactccat aacagcttcg acaatatttc tggaatattt tccgcaagca
      961 attactatgt ccacaccttt ttcaacacat aattttccca ccttgtagtg gtattcgtcg
     1021 gaatactcac caagttccaa catatctccc aatactgcaa cttttctgtt ttcaaaggat
     1081 tcaagaactt ccagagaagc cttcatcgac tcataacttg cattgtatgc atcgtcaatt
     1141 attgtcacat tcctgttttt tagagtgaaa atattctgcc ttatgctgtc ccctgtataa
     1201 ctttcgcagg cagaaactgc ctcctcaaca gggacaccca gacattttgc aatgcagatt
     1261 gccaccaggg aattgtacac attgtgcttg cctttaagtt tcaaggatac agtgtactct
     1321 ttgcctgact catccgtgaa tacatatatg ctgtttgaag catcataatc tatgaccctc
     1381 atatagttgt cttcatcaaa acccacctta aaaacagcaa catcaccgaa atccccctgc
     1441 ttcagatatt cgtcatcccc atttacaaca aggacgccat ctcctgccat tccaattgtg
     1501 atttcggatt ttgcttttaa gatgttgtct ttcgttccaa gaaactccag atgatttgag
     1561 ccaatattgg taattaccgc atgggtaggt cttgcaatcc tggaaagata ctctatctga
     1621 ttcagtcctc tcattcccat ctcaaccact attgcctctt cctctacaag gttgagtatt
     1681 gtcttgggaa ggcctatctc attattctca ttcagtttgc tttttaatgt tctgaacttt
     1741 ttactcaaaa cttcaaatat ataatctttt gtgctggttt tcccaacact gccggtaaca
     1801 cccacaacaa tcaaaggttt tccaagcttc tcttcataaa aattcctgta aaaggaggca
     1861 atatcgccat aagctgtcaa tgtgttttct acctgaataa tattagtatt accgccgtca
     1921 atactcttat gtaccagaca cgcaactgct ccactgttaa aagcatcctg acaaaaatcg
     1981 tgaccgtcaa atttttctcc ttcaatacaa acgaaaagag aaccttttgt tactcgtctc
     2041 gagtcggtta caatgcttgt aattttttca tcaattattt tctcgtttag cagtttgcca
     2101 ttggtactgg ctagtatctg tttaacagtc aattcaagca ttaactacac ctcttgtctt
     2161 cttcgtccat tctggcaagt atatccctaa caacctcacg ttcatcaaaa tgaattacct
     2221 tgtctctgaa aatctggtag gtttcatgtc cctttccagc caggattata acgtcgccag
     2281 gcagtgcgtt tctaagtgca tattcaatgg cttcctttct gtccacaata caaatatatt
     2341 ccgcacctgt gggtttgata ccctcttcta tttccttgac tatcttttca ggatcttccg
     2401 ttctgggatt gtcggatgtt attatggtaa agtccgcata tttgcctgct gtctcgccca
     2461 taagtttcct aggcctattt ctgtcaccgc cagctccaaa aagaaacacg caacgctttg
     2521 caaattcctt aactgttgtc aaaatgttaa taaaggaatc tggattgtgg gcatagtcaa
     2581 taatcacggt aaagtccctg tttgttggaa caacttccgc cttgcctctt actgtaacat
     2641 ctctgatgcc ttcaacaatt gtatccatgt caataccttc aatatggcac gttccaatcg
     2701 ctgcaagaga attgtacaca gaaaaggttc ccggactgga tacgtaaaca tgcctcttgt
     2761 ccgtcttgcc ttcttttgtc ccgtacaaaa catcatattc aacatggtcc gcatacttct
     2821 tgatattcac agccctgtag tcacaatctt tttctatgcc gtaagaatac actttctttc
     2881 catttgaagc catcaaatcg aaatattcgg aatctgcatt tattaaagaa ttttcgctca
     2941 ttccaaacaa catggcttta gattttgcat aatcctccat gtcaggatgt tccgtctccc
     3001 caatatgatc ccgtgacagg ttcgtaaata tggccgttga ataggtgcaa aactcgaccc
     3061 tgcccagttt caaaccctga gaggaaacct ccataacgca tacatttatc tccctgttcg
     3121 ccatttcatt cagcaatctt tgcaaatcgt ttgcctcagg cgttgttctt tccgtataaa
     3181 gaatctcatc atctatcatg ttgtacatgg tcccaattag accggttttg tactttgcct
     3241 tttccaatac ggactttata taaaatgtgg tggatgtctt tcctttggta cctgttacac
     3301 caatcagctt gagccttcct gacggatgat tgtacaatct gtccgccaga tacggaagac
     3361 tttttcttgt atcttttgtt ttgactattg aaacatcccc tgtaacatcc acatcatccg
     3421 tcacaagaag tactccacaa ccattgttta cagcctgctg ggcaaaagca tgcccatcct
     3481 gaacataacc ttttatgcag acaaacatgc tgcctttact ggccagtcta ctgtccacca
     3541 caatatcctc tatttccctt tcagcatttc ctttaatata ctctatatca agacctttaa
     3601 ccagctcatt cagcttcata aaacccctca attcttaagc cgtctcaata tcttcattaa
     3661 gcagctttat ttcaactact gacccaattt caacctgctt tccaggctca atgctctgcg
     3721 atacaacagt accaaggctt gaagctctca tgttaagacc cctttccttc aacgcactgt
     3781 acgcctcctc aagagtaaga cccataagat ctggtactgt aatcttttcc tttacagact
     3841 ctttctcggt gtatatgacc accattgaat cctttgacac cagagctcca ggtctaggca
     3901 tctgctcata gattctggca tcgggatcag tatagtcccc tactattcta tacgaaagat
     3961 tgttcttttt caactcgtca atagcttcag ataatgtcaa ttctgaaaga tcgggcacct
     4021 tgtacttcac aagcatctta tccttgtcaa gctcggtgta tcttctttca acttccatgt
     4081 actccagtat cttctcaata agttctcgtg ccacaggcgc tgcctgtctt cctcctgatg
     4141 taaccccaac ggttggatgg tccagtacga ttagtatcac tatttgcggg ttgtctgcag
     4201 gagcaatacc acagaatgaa gccacatatc tgcctgttgt atcggtttca ggagtctgtg
     4261 atgtaccggt ctttcctgcc acacggtatc ctgttacgta tgcattgctt cctccgccag
     4321 aagcaacagt ttcttccagc atggaaagaa gagtattggc agtctctttg ctcaaaacct
     4381 gccttactac ctttgtatcg tatctcatga ccacgttgcc atgctcgtcg gttagttcct
     4441 tcactattct tggagtcaaa agatttccac catttgctat ggcactgtaa gctgtgatta
     4501 gctgaatcgg agtaatctgc agtctctgac caaaagccgc aaccgccata tcaatttcct
     4561 tgggatcctt atgtatgatg ctgtctgctt caccggtaag ccccagtcct gtttttcttg
     4621 taaagccaaa agcatccaga tatgaataaa actttttaat accaagcttg agggaaagct
     4681 tagcaaatac agggttgcag gaattcttga tggcttcaag aaaggtctcg tttccgtggt
     4741 cgtaagtttt cctccagcat cttatggtcc agccagacag cttcaatgga gcgtcattga
     4801 caattgtgct gggatttgct attccctcct ccaatactgc agcagaggta atggccttga
     4861 acgtcgaacc cggctcatag gtatctgtta gggccttgtt ccgccaaaca gtagtgctta
     4921 gtatttgtac ggattcggaa gagtttccgg tccaagatga tggatccatg tccggaggac
     4981 atgcgtaagg gtcatttaga ttgaaagttg gccaggatgc cattgcaagg atgtctccgt
     5041 tattcggatt cattacaata cctgcagcac cgccgattac cccttggtct ataacggctt
     5101 tctgcaaagc ctgctccacc atgtattgta ttgttatatc tattgtaagg gtggcattat
     5161 atccgtttac cggctctatt cttctttcag catcaaaggg cagttctaca ccttttgcat
     5221 ccacttccgt tacaatcctt cctgctttgc ctgccagcat tttgtcaaga gcaacctcca
     5281 caccacatac aagcccctgg tcatcacgac ctgtaaagcc taaaatgtgg gcggcaatgg
     5341 agttgtttgg atagtatctc ctaacatcct cgtccacatg tacgcctttt atcttgttct
     5401 tctttatcca tccctccacc tgttcgccta cttcaacatc tatctttctt gcaatttcct
     5461 tgtacctccc tacggactga actttctcta gcacagtctc gtaatttaaa tcaagaatat
     5521 ccgcaagggc tcttgccact agttcctgat atgctttgac atctcctcgc gcttcacctt
     5581 ccttgataat tgtctgctgg ttgacagaaa cctgtcttgc attaacactt atcgccagaa
     5641 tattcccgtt tctgtcgaat atcgtgccgc ggtttgcagg tattattcta ccgttgtttt
     5701 gctgtagata agccctcttt tgatactctt cacctcttac gaattgtatc catgcaatgc
     5761 gcaaaagaag gccaaatatt ataatgataa aaataacaac aagagcagca cttctattgc
     5821 ctgctcttat cttcatcaga gtttcatttg atttcttatt attcttatta aaaaaagaca
     5881 ttttgcatcc tcccagaaag gtacatgtgt gtacatccta taaatatgcc tgacttgtca
     5941 acatatacct aaaaacgctt taatttcatc aagcacattt ttataccagg gggtgttgtt
     6001 ttcatctttg gtaaatttca tctcgtgtat ttctgtttga tccgtaggaa caacctttat
     6061 tctcactatc tgataactct gaggtttctg cattcccaac tcgtttattg caatctgact
     6121 tatttcagcc
//
LOCUS       DAONVY010000066         6125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9620_length_6125_cov_3.866557, whole genome shotgun sequence.
ACCESSION   DAONVY010000066 DAONVY010000000
VERSION     DAONVY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9620_length_6125_cov_3.866557"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..663
                     /locus_tag="PLI11_02865"
     CDS             184..663
                     /locus_tag="PLI11_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPZ51838.1"
                     /translation="MGTYDESSLSDMIMKYSSMILRIAVQNTNDFYVAEDITQEVFIK
                     LMTCKKAFDNENYVKAWLIKVTLNMCKNHTKSSWRKRTVSIQEREATQFFVLPEDRYI
                     FELITKLPDKYKNVLYLYYYEEYTVPEIAELLDKKINTISTWLRRARIELKKRIEEE"
     gene            666..1586
                     /locus_tag="PLI11_02870"
     CDS             666..1586
                     /locus_tag="PLI11_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51839.1"
                     /translation="MDDNSINKYLELMSKVKYDSNDNEKLFVSITKLLNNEDNIQIEK
                     LNSMNTTSMIGADIYPKRHSLIASIATAAILFVLLLVILFPDTITSLYERFIGVSPTP
                     ASQTTPNFQTNYTATPILTPKKTPFQTEQQTPSPTVRNVYIRDLTKNEEILNNIYHTI
                     FPDRTDYIAYWMDDNIFWAPVDQLHYRHLYCIRINSTTGEVISKRYDISYLEDSLSYR
                     IISINGVTYLALATNTWEHGELYFSEIVNCETMEPVWNIQDLYPDNWESYKVVLNENG
                     IDLYHGERLDTYLVNFELVRRIPYDELIYA"
     gene            1740..1919
                     /locus_tag="PLI11_02875"
                     /pseudo
     CDS             1740..1919
                     /locus_tag="PLI11_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
     gene            complement(2013..2306)
                     /locus_tag="PLI11_02880"
     CDS             complement(2013..2306)
                     /locus_tag="PLI11_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51840.1"
                     /translation="MGKEYKALDNLLIMREDGEIDKETFLRRKEIRETNIKVIKEKIN
                     ELEERIKRSQAMDDKRRLANIENFFTKWEQTDSIELKNQFLRMIIDNIIYKRE"
     gene            2489..3397
                     /locus_tag="PLI11_02885"
     CDS             2489..3397
                     /locus_tag="PLI11_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51841.1"
                     /translation="MYGEVRNRGRAFHDGYNDRTKGALNWGFNIAEQWEYAMEQDPRI
                     IFVTGWNEWTMGRVRGSKERPVTFIDQANQEFSRDIEPMRDGHFDDYYMQLVDYVRRF
                     KGMDEVKPGMRKTIDIHGQFAQWEDVEPTFHDLPFGNCHRDHFGVGGDRYVNDTGRND
                     IDRMKICYDDENVYFYVSTFDRMQRYSFTPWRRLFLHVEGNDFIGWERYQYAANLELV
                     DGDNSIVYKSLGAWRFVPIGRAPMKHEGSEMMLMVPRKLIGLEKTPFEFQFKWADGIA
                     GDWTIEDFYLNGDTAPYGRLNYVYRS"
     gene            3596..4069
                     /locus_tag="PLI11_02890"
     CDS             3596..4069
                     /locus_tag="PLI11_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51842.1"
                     /translation="MLISLSYYKIRIDAVIHDGKRSSNDMYKWDNAGDIASMSNSLSL
                     ISSSSPAIVSGSIEYWNIVLPNTGASEPDSDRDCWQGDIDWYEFMVNEAGVYVIRTYG
                     NNGLYGVLYEGKTFMQSSSGVYMQVALSPGKLYKLKILGVSSGNNGDYTLSIVKS"
     gene            4088..4915
                     /locus_tag="PLI11_02895"
     CDS             4088..4915
                     /locus_tag="PLI11_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51843.1"
                     /translation="MKKGYLLLVAVSFVVVLVLAVSYSNLPFSAGQSGQDMEMEDTAG
                     KYDDTVSVEQLNTLLEELDVELHDGEECDLTVLNHVLSSMDLDTCVDFKSEPVLHDGL
                     SKEDVHKLLGDPEYELDNVWIYSKGASLLVFNDGKLGKLARLGTDSEGFYIMEIRNAL
                     NIEDVRKLIQEKDELRYTILSSDEELSKLAGLSQDELFGLIGYPDVYSPSSANPYIQY
                     VTDKRTVTFKFDNGVGLCYTEDNETGESNYVFMNDYFKQEFEKWQQCYDKYMESMKK"
     gene            complement(4952..5104)
                     /locus_tag="PLI11_02900"
     CDS             complement(4952..5104)
                     /locus_tag="PLI11_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51844.1"
                     /translation="MESSVFFPAYNGLVILATTVAGSMFFKEKLTTRQYASIVVGILG
                     VVLVNI"
     gene            5906..6079
                     /locus_tag="PLI11_02905"
                     /pseudo
     CDS             5906..6079
                     /locus_tag="PLI11_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
BASE COUNT         1981 a         1079 c         1330 g         1735 t
ORIGIN      
        1 ctgcaagctc aaaggcagac aatggcaaga tgtacaagat ctacataatt gcctctaaat
       61 taataggcat taatatggta acattaaaag tataaactga tcacttaaag ttttttctcc
      121 tgcaatgaac ggttttggtc tagttaattg ttttatatat gttaagaaaa ggagtttgat
      181 ttcgtgggta catatgatga aagttctcta agtgatatga taatgaaata tagttcaatg
      241 atattacgaa ttgcagtaca aaacacaaat gacttttatg tggcagaaga tataacccaa
      301 gaggtattta taaaattaat gacttgcaag aaagcgtttg acaacgaaaa ctatgtaaaa
      361 gcatggctaa taaaagtaac cctaaatatg tgtaaaaacc acactaaatc ttcttggcga
      421 aaacggactg tatcaattca agaaagggaa gcaacccaat tctttgtatt acctgaggat
      481 aggtacatat ttgaattaat aacgaagctg cctgataaat acaaaaacgt cttatatctt
      541 tactattacg aagaatatac agtacccgaa attgcagagt tgttggataa aaagataaat
      601 acaataagta catggctgag aagagcaagg attgaactta aaaaaagaat tgaggaggaa
      661 tagtgatgga tgataatagt ataaataaat atttggaact catgtctaaa gtaaagtacg
      721 atagtaatga taacgagaag ttatttgtgt ctataactaa acttcttaat aatgaagaca
      781 acattcagat agaaaaactt aacagtatga atactacttc tatgattggc gctgatatat
      841 atccaaaaag gcacagtttg attgcaagca tagcaacagc agcaatacta tttgttttat
      901 tgcttgttat actttttccg gacacgataa caagtcttta tgaacgattc attggcgtga
      961 gtcctacccc tgcttctcaa acaacgccaa atttccaaac taattatact gcaacaccaa
     1021 tactcacccc gaaaaagact ccattccaaa cagaacaaca aacacctagt ccgacagtta
     1081 gaaatgtata tataagggat ttgactaaaa atgaagaaat cttaaataac atataccata
     1141 ctattttccc tgatagaaca gactatatag cttattggat ggatgacaat atattctggg
     1201 caccagtcga ccaattgcac tataggcatt tgtattgcat aaggattaac agcactacag
     1261 gtgaagttat aagtaaaaga tatgatatat cttatttgga agacagttta tcatatagga
     1321 ttataagtat caatggagtg acataccttg cattagccac gaacacatgg gaacatggtg
     1381 aactctattt ctctgaaatt gttaattgcg agacaatgga gccggtatgg aatattcaag
     1441 acttataccc agataattgg gaatcatata aagtagtact gaatgaaaat ggtattgacc
     1501 tatatcatgg agaaaggtta gatacatatc ttgttaattt cgaactagtt agaagaatac
     1561 cttatgatga attgatttat gcatagtgta tgcagggaca gctctgaaag acgagaataa
     1621 aagagaccat aaaaaggtta taatacaaat agcaacttgt tatatgagac tcgctaataa
     1681 taagtgtcaa agttatgcgt atagtgatga gaacgctaga aatcaaggac gtgataccga
     1741 tgtcaaccag caaacaagcc aatagactga ttcacgagaa atccccctac ttgttgcaac
     1801 atgcatacaa tcctgtggac tggtttccct ggggtgaaga agccattgta aaggcgaagg
     1861 cagaggacaa gcctgtcttt ctttccatcg gctattccac ctgtcactgg tgccatgtct
     1921 gcaatataca ccaaacgaca atgttgtaat atcaagcgtt ccgtccctgc gctatacgcc
     1981 taacaaaaat ttacaacgtt ctcaatctca tctcactctc ttttatagat aatgttgtcg
     2041 ataatcattc tcaaaaactg atttttcaat tcaatgctat ctgtctgttc ccattttgta
     2101 aaaaagtttt caatattagc cagtcgcctt ttatcatcca tggcctggct tctcttaatt
     2161 ctctcctcga gttcatttat tttttctttt atcactttta tgtttgtctc cctaatttct
     2221 tttcgcctta aaaatgtctc tttatcgatc tccccatcct cacgcattat caatagattg
     2281 tctagggctt tatactcctt tcccatctga tccctatgtt gctgcagggc ggcctacagg
     2341 actacggtgt catcaacatt aacctgttgc gtgccactgg attgcatttg agcgtccgca
     2401 gcatgtattc agggacaagg acggcaatcc cgaaatcatc agtgtttccg ttgctcagca
     2461 tccacagata aagtttgggg attcggcgat gtatggagaa gtcaggaacc gtggaagggc
     2521 tttccatgat ggatacaacg acaggaccaa gggtgccctg aactggggat tcaacattgc
     2581 ggagcagtgg gagtacgcga tggagcagga cccaaggatc atcttcgtga ccggatggaa
     2641 tgaatggacc atgggaaggg tccgtggctc aaaggagaga cctgtcacgt tcatagacca
     2701 ggcaaaccag gagttcagca gggatatcga gcccatgcgc gatggacatt ttgacgatta
     2761 ttacatgcag cttgtcgatt acgtccgcag gtttaaaggt atggacgaag taaaacccgg
     2821 aatgaggaag accatcgata ttcatggcca gtttgcccag tgggaggatg tggagccaac
     2881 gttccatgac cttccttttg ggaactgcca cagggatcat ttcggcgtag ggggagacag
     2941 gtacgtgaat gacaccggca ggaacgacat tgacagaatg aagatctgtt acgatgacga
     3001 gaatgtgtat ttctatgtaa gcactttcga caggatgcag aggtattctt tcacaccatg
     3061 gaggaggttg ttcctgcatg tagaagggaa tgacttcata ggatgggaaa ggtaccagta
     3121 tgcggcgaat cttgagctgg tggatgggga caactccata gtttacaaaa gccttggagc
     3181 gtggaggttc gtgccaatag gcagggcccc tatgaagcac gaaggcagtg agatgatgct
     3241 tatggtgcca agaaagctta ttgggcttga aaaaactcct tttgagttcc aattcaaatg
     3301 ggcggatgga atagccggtg actggacaat agaggatttt tatcttaatg gcgatacagc
     3361 accttatggc aggctgaatt atgtttacag gagttaatgg gagagtcgcg gaatgtacga
     3421 tggaatgaag caggcaggtg ggaaatcacc tgccggttct gttttatgta catgccgggc
     3481 aatagatagg ttgtatgcat ttaaaaatat taacaaaaag ttcaaataac atatgtttac
     3541 aatttaatgg aattagctat tatggtaagt gaggtgaagt ttaatatgaa aaaacatgtt
     3601 aatcagtttg tcttactata agatacgaat tgatgcagta attcatgatg gaaaaagatc
     3661 atcaaatgat atgtataaat gggataatgc tggtgacatt gcgtcaatga gcaattcttt
     3721 gtcgttgatt tcatcttcct cacctgctat tgtctcgggt agtattgaat attggaacat
     3781 agttctacct aatactggtg caagtgaacc ggattcagat agggattgtt ggcaagggga
     3841 cattgattgg tacgagttta tggtaaatga agctggcgtc tatgttatac ggacatatgg
     3901 taataatggt ttatatggtg tgttatatga aggaaagact tttatgcagt ctagtagtgg
     3961 cgtttacatg caagtagctt taagtcctgg aaagttatat aaactcaaaa tactaggtgt
     4021 cagtagtggc aataatggag actatacatt atcaatagta aaatcttaat agaaaggaga
     4081 atgatatatg aaaaaagggt atcttctgtt ggttgctgta tcattcgttg ttgtattggt
     4141 cttggcagta tcttacagta accttccgtt ttcggccggg cagtccggtc aagatatgga
     4201 aatggaagac actgccggga agtacgatga tactgtttct gtggaacaac tgaacacgct
     4261 gttagaagag ttggatgttg aactccatga cggcgaagaa tgtgatttga cagtcctaaa
     4321 tcatgtgctg tcaagcatgg atttggatac ttgtgttgat ttcaagagtg aaccggtttt
     4381 gcatgatggc ttgtcaaagg aagatgtgca caagcttctt ggtgatccgg aatacgaact
     4441 tgacaatgtg tggatatatt ccaagggcgc aagtttgctt gtcttcaatg acggcaagct
     4501 tggcaaactg gctcgtctgg gcacggatag tgaagggttt tacattatgg aaatccgtaa
     4561 tgctttaaat attgaagatg tcaggaagct catccaggaa aaagacgaat taaggtatac
     4621 catactcagc tccgacgagg aattgagcaa gctggccggg ttgtcccagg atgagttgtt
     4681 tggtttgatt ggatatcctg atgtttactc gccttccagc gcaaacccgt acatccaata
     4741 tgttaccgac aaacgtactg ttaccttcaa gtttgataat ggcgttggat tgtgctacac
     4801 ggaagataat gaaacaggtg aatccaatta tgttttcatg aatgattact ttaaacagga
     4861 gtttgagaaa tggcagcaat gctacgataa gtacatggaa agcatgaaga aataaagttg
     4921 ctgggctgtt gctaatgtca acggcccttt ttcatatgtt taccagaaca accccaagta
     4981 tgccaacaac tatgcttgcg tactggcggg ttgttagttt ctccttaaaa aacattgatc
     5041 ctgcgactgt tgtggcgagt atgaccagtc cgttgtatgc cgggaaaaac acgctgcttt
     5101 ccattttgcc caccaggtac aagttgatga tgttgattat ggcatacctg gccccgctcc
     5161 gagttgtggt ttggaattcc ttgttcagaa agttatttca tgaaataact ccaattttta
     5221 atgaactgca atatttaaag gaacaaggcg aactctggag aaacatcaag gttaaagagg
     5281 aacgtttata gattaacaca aaagatggta gaagaaaacc ccaccaagag gtgagtcgtc
     5341 ctcgtccttg taacaatacc aaattgatta attgctattt attacaatta atcaatttgg
     5401 agtaaaatta gaactttgtt cactaacgga ccgggacctc tccccactac aagcttaact
     5461 atagttcagt ctatgattaa gatgtcaaca aaagtcctct tacataaaat ttgaatagat
     5521 aaaggtaatc gtggcaccat caacaaggca cccgacgccc aagttattgc aaagtttctt
     5581 gcgagaagcc gaggaaaaaa aagaagacgt ggtgcgaaat atattgaaca tatgtgcttg
     5641 taaagaagaa agtgtattcg aaatagtgca gcagggtata aacaagggag ttttactgta
     5701 taataatgaa ggagaggatt caagtgttta attcaacaga atcaataaga agcatgctaa
     5761 ctcaacaaca cagcagttcc tcttgcataa gacattatgc caaaatttat agcgtattgt
     5821 ataatatata cgaatccagc tcataccaaa taatgtcata aacagcgtat agtgcaatta
     5881 tcataaacaa taggaggtgg ttccgatgtc aactaacaag caagccaata gacttatcca
     5941 cgagaaatct ccctacttac tgcagcatgc atataatccc atcgactggt atccctggtg
     6001 cgataaagcc tttgaaaagg caaaactgga aaacaaacca gtatttctgt caattggtta
     6061 tagcacctgt cactggtgtc aagtctgcaa ataacagcaa ttaatgagta tttaaaacgg
     6121 ccatt
//
LOCUS       DAONVY010000067         6090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9676_length_6090_cov_3.321292, whole genome shotgun sequence.
ACCESSION   DAONVY010000067 DAONVY010000000
VERSION     DAONVY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9676_length_6090_cov_3.321292"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..654
                     /locus_tag="PLI11_02910"
     CDS             52..654
                     /locus_tag="PLI11_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51845.1"
                     /translation="MLKEFGPIKIDLQLFADGEGAEDDPNKSQETNQDTDPNTEPGSV
                     ANDNPPKTQEELDAIIEKRLERERRKLSKQQRQQQKQANTQNPDEGDEGTTQTTPELE
                     NLNRELLMARAQLEAFRSGVRSDVVEDAVYLALRTAEKDGEVDEEDIKDALKSVLKRH
                     PEWKSDSSKQGGGFKVGATGGKHQEPATNDVLAEIFGNKK"
     gene            677..1558
                     /locus_tag="PLI11_02915"
     CDS             677..1558
                     /locus_tag="PLI11_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HPZ51846.1"
                     /translation="MANVINYATTFERELKQKYTREALSSGLTTERIQFVNANTIKIP
                     YVIVGGYKDHGRQGGFNRQSVENKWMTKTLAFDRNIEFFVDSMDVDESNEVVAAANIT
                     NTFVTEQAIPEMDVYRFSKLYADFVDLGGVVDTTALTASNILDVFDNWMEQMTDKEVP
                     VDGRILYVTPSVNKLLKTADAVQRQINVSSAGGLDRRIVSLDNVNIVEVPSGRLKTSY
                     DFTDGFKPAADAKQINMILVHPSSVIACDKHSYIRLWAPGTHTQGDGYLYQNRKYGDL
                     FVLDTRKDGIAINTDAE"
     gene            1572..1820
                     /locus_tag="PLI11_02920"
     CDS             1572..1820
                     /locus_tag="PLI11_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51847.1"
                     /translation="MYAKRANRQYTITEGEKDTYIALGYDIYDGKGKLIAHGVGKTVS
                     YTKYENALREIEKLTKANEKLQKELEKLQKEPEKDKKG"
     gene            1826..2224
                     /locus_tag="PLI11_02925"
     CDS             1826..2224
                     /locus_tag="PLI11_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51848.1"
                     /translation="MYVSLEEYEVLSNDKAVGPEKLESVLRDAERHIDSLTFNRIKGL
                     GFDRLTDFQRELVKQSVVDQADFKHQYGIYLENPLSSYAINGVSMTWDTSKVLQVRGV
                     YTTASIYGVLRQTGLTYRGGVTDEMARIGA"
     gene            2199..2534
                     /locus_tag="PLI11_02930"
     CDS             2199..2534
                     /locus_tag="PLI11_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51849.1"
                     /translation="MKWPELVPDSMCNTPITILFEDGINEDGSPKKTVIFDGKCNYSE
                     KAKQIMNAERQLIQLQAVALMNGDIAPGKNIAGEAVINNGQIVRSIYAFSRGYNVDGS
                     VNFTRLELM"
     gene            2534..2692
                     /locus_tag="PLI11_02935"
     CDS             2534..2692
                     /locus_tag="PLI11_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51850.1"
                     /translation="MKIRIELDAMAIKKLEEAAVESDSNGVAVRLVKQAHHRPEGCIT
                     TRSITFKL"
     gene            complement(2903..3229)
                     /locus_tag="PLI11_02940"
     CDS             complement(2903..3229)
                     /locus_tag="PLI11_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HPZ51851.1"
                     /translation="MKLVLAIVNNEDSAIASSALTQEGFFVTKLSTTGGFLMVGNTTL
                     LIGTMDDKIDKIKEILSKYCSTRKQTNPSYSSLGRNVKNDSLPEDVTVGGATIFVLGV
                     EDFSKL"
     gene            complement(3262..3750)
                     /locus_tag="PLI11_02945"
     CDS             complement(3262..3750)
                     /locus_tag="PLI11_02945"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HPZ51852.1"
                     /translation="MARLYISGDEAKITLKEHYRVDVEFYNGVKFEDLEPRRLFPISG
                     LRKYITLLDKDGNEKCIIRDLDTLMPESREAVEKCLDEYYLVPKILRIIDTEEKYGIL
                     KMKCETERGIRTFDVRNRYSDIKVLYDNRVLIRDSDDNRYEIPDHTRLDKHSKKLLYA
                     EL"
     gene            complement(3767..6025)
                     /locus_tag="PLI11_02950"
     CDS             complement(3767..6025)
                     /locus_tag="PLI11_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ51853.1"
                     /translation="MVEYENALKNKPIPETIRKLVEAKLVDDERLLFVIIGDLTLEDK
                     YGESALAVTDKKVMSVDSSHADGILTYEYKEIKDASIKRMYGNAQLNIELNDGRILRF
                     FRFTYTVTTLCEMASSFIVQIGKGSPLKDELEIAEATFEKLMSVCPTCGRTLIRPGAD
                     CIKCQSKKKIATKFIKYIIPEKWPLLFSLFLSVITTAIALLPPYITKELLNSILPNKD
                     YRALTIVVIVLLVSYIVHYSVAAYRGYLLRMSGNRIVESLRNDVYQKAQYLPMKFYDK
                     TTTGSVINRINGDTENIRGFIIRVTQEILVQFMLLIGIVVIMLTMHWKLTLLSLIPVP
                     FVVIGAKVFAEKIRPYYIRIWRRGAAVSSVLTDTIPNIRVVKSFAKEQSAVKKFERYT
                     AEWRKVDTNSAKITNTFPNAVALFVTLGSLLIWSIGGKWVISGSLSVGLLVSFISYTA
                     MFYGPVGFFANFSDVYQSALTSIERILDIIDAEPEPDYGKGRVLGEVKGKIEFRNVNF
                     SFDRTKKVLSDINLVIEPGDVVGIVGTTGSGKSTLVNLIMRFYSDYEGDILLDGVNIK
                     DIDMEFYRSCIGFVQQDPMMFSDTIFNNIAYGVPDAHVEQVIHAADIANAHDFIAKQP
                     DGYDSVLGERGVGLSGGERQRISIARAVLKNPRILIFDEATASVDSETENLIQQAIDR
                     MISGRTTIMIAHRLSTLRKANKIVVVDQGRIIECGSHEELMALKGKYHKLIEIQSLSE
                     KLQRDKEKEKFE"
BASE COUNT         1873 a         1218 c         1291 g         1708 t
ORIGIN      
        1 aactgcacgg tacaactatt aagccgacat gctataaaag gaggagcaat aatgcttaaa
       61 gagtttggac caataaaaat tgacttacag ctttttgctg atggtgaagg cgctgaagac
      121 gatcctaaca agagccaaga aactaaccag gataccgacc ccaatacgga gcctggaagt
      181 gttgcaaacg acaaccctcc taaaactcag gaagagttgg atgcaattat agaaaaaagg
      241 cttgaaaggg aacgcaggaa gttatctaaa cagcaaaggc aacagcagaa acaagcaaac
      301 actcaaaacc ctgatgaggg cgatgagggg actacacaga caacccccga acttgaaaac
      361 ctaaatagag aattgcttat ggcccgtgca cagctagaag cgtttaggag tggagtaagg
      421 tctgatgtgg tagaagatgc ggtatatttg gctttacgca cagccgagaa ggacggagaa
      481 gttgacgagg aagacattaa ggatgcttta aagtccgtac ttaaaagaca tccggaatgg
      541 aagtctgaca gcagtaagca aggtggaggg tttaaggttg gagctacagg tggtaaacat
      601 caagagccag ccaccaatga tgtacttgcc gagatattcg gaaataaaaa ataacagaca
      661 aaggggataa gtaaatatgg caaatgtaat taattatgca actactttcg aaagagaact
      721 aaagcaaaaa tacactagag aggccctttc aagtggtcta accacagaaa ggatacagtt
      781 tgtcaacgct aacactatta aaatacctta tgtgattgtt ggaggataca aggaccacgg
      841 tagacaaggt ggttttaata ggcagagtgt tgagaacaaa tggatgacca agaccctggc
      901 ttttgacaga aacatcgagt tctttgttga tagcatggac gtagatgagt caaatgaggt
      961 tgtagctgca gcaaacataa caaatacttt tgttaccgag caggccattc cagagatgga
     1021 cgtatacagg ttctcgaagc tgtacgctga ttttgtagac cttggtggag tggtggacac
     1081 tacagcatta actgcgtcta atattctaga tgtctttgat aattggatgg agcaaatgac
     1141 ggataaagag gttcctgttg acggaagaat tttatatgtg accccatccg ttaataagct
     1201 actcaaaact gctgatgcag tacagagaca aatcaacgta tctagtgctg gtggccttga
     1261 taggagaatt gtaagcttgg ataacgtgaa tatagtggaa gtgcccagcg gtaggctcaa
     1321 gacatcctac gactttacgg atggcttcaa gccagcagca gacgctaagc aaatcaatat
     1381 gatacttgtg catccttcat ctgtcattgc ttgtgataaa cactcctata tcagactatg
     1441 ggctcctggt acacatactc agggagacgg ttacctgtat cagaatagaa aatatggaga
     1501 tttgtttgtg ctagatacgc gcaaagatgg catagcaata aacacagatg cagaataaga
     1561 ggttatgttg agtgtatgca aaaagggcta ataggcaata tacgattaca gaaggagaaa
     1621 aggatactta tattgcatta ggctatgaca tttacgatgg taaaggtaaa ttgatagctc
     1681 atggagtcgg taaaacagtc tcctatacta aatatgaaaa tgcgttaagg gagattgaaa
     1741 aactaacaaa agctaacgaa aagctacaga aagagctaga aaaattacaa aaagaacctg
     1801 aaaaagacaa aaaagggtga tgtaaatgta cgtgagcctt gaagagtatg aagttttaag
     1861 caacgataag gctgttggtc ccgaaaagtt ggaaagtgtt ttgagagatg ctgaaaggca
     1921 catagacagc cttactttta accgaataaa aggtcttgga ttcgacaggc tcacggactt
     1981 ccaacgtgag cttgtcaaac aatcggtagt ggaccaggcg gatttcaaac accaatacgg
     2041 catttattta gaaaatcctt tatcgtcata tgcaataaac ggtgtttcaa tgacgtggga
     2101 tacatcaaaa gtcttacaag tacgtggggt atatacaaca gccagtatat acggagtact
     2161 tagacaaaca ggtttaactt atcggggggg tgtaacagat gaaatggcca gaattggtgc
     2221 ctgattcaat gtgcaacacc cccatcacaa tcctatttga ggatggaata aatgaagatg
     2281 gcagtccgaa aaagactgtc atttttgatg gcaaatgcaa ctattccgaa aaggccaagc
     2341 agattatgaa tgctgaaaga cagcttattc aactgcaagc tgtagctttg atgaatgggg
     2401 atattgcacc aggtaaaaat attgcaggcg aagctgtcat aaacaacggc cagatagtaa
     2461 gaagcatcta tgcattttct agaggctata acgtagacgg gtctgtaaat tttacaaggt
     2521 tggagctgat gtaatgaaaa tcaggataga attagacgca atggccataa agaagctgga
     2581 agaagcagct gtggagtccg attccaatgg agtagctgtt agattagtaa aacaggctca
     2641 ccacaggcca gaaggctgta ttaccacccg aagcataact ttcaaactgt aaacaaccct
     2701 aacgcaaaag gactttggct agcggattgg gtggaaggag ataaatcaga cttgatagaa
     2761 aaacttttaa ggaactctac aaaaaggagg ctgacttatg actacttaac tgtaaaggta
     2821 acttatagat acggtgataa tactattcaa gccaaatcca tattatgtat aaaaaaaccg
     2881 ggcaatttgc ccggtttgtc atctataatt tcgaaaagtc ttccacaccc aaaacaaaga
     2941 ttgttgctcc tcctacagta acgtcttctg ggaggctgtc gttttttaca ttcctaccga
     3001 gtgaactgta cgaaggattc gtctgttttc gtgtagaaca atactttgac agtatctcct
     3061 ttattttgtc aatcttgtca tccattgtac ctataagaag ggttgtgttt cctaccatta
     3121 aaaagccacc agttgtggat aacttggtca cgaaaaaccc ttcctgagtc aatgctgatg
     3181 acgcgattgc agaatcttcg ttgttaacta ttgctaaaac aagcttcata tctctcaacc
     3241 tcccattatt tatttttttc atcaaagttc tgcatagagt aacttcttgc tgtgtttatc
     3301 caagcgggta tgatctggaa tctcatatct gttgtcgtcg gaatctctaa taagaactcg
     3361 attatcgtat aagactttta tgtcgctata cctgtttctg acatcaaaag ttctgatgcc
     3421 acgttcggtt tcgcatttca tttttagtat accatatttt tcttcagtgt caataattct
     3481 gaggatttta ggtacaaggt agtactcgtc caggcacttc tcaacagcct ctcttgactc
     3541 aggcatgagg gtatccagat ccctaataat acacttctcg tttccatctt tgtctagcag
     3601 tgttatgtac tttctcaaac cacttatggg gaacagtctt cgtggttcaa ggtcttcaaa
     3661 cttgacgcca ttgtagaact ccacatcaac cctgtaatgt tccttaagag tgattttcgc
     3721 ttcatcaccg cttatatata atctggccat tcctttctcc tccttgctac tcaaacttct
     3781 ctttctcctt gtctctctgc agcttttcgg aaagagactg gatttcaata agtttatggt
     3841 acttgccttt gagagccatc aattcctcgt gcgaaccgca ctcaattatt ctgccctggt
     3901 caacaacaac aatcttgttt gcctttctca aggttgacag tctgtgggca atcatgatgg
     3961 ttgtacgtcc gcttatcatc ctgtcaatag cctgttgaat aagattctcg gtttcagagt
     4021 caacactggc agtagcttca tcgaatatga ggattcttgg gtttttcaat accgcacggg
     4081 caattgaaat acgctgtctt tcaccgccgg acaggccaac gcctctttct ccaagtacgg
     4141 agtcataacc atcaggctgc tttgcaataa agtcatgtgc atttgcaata tctgcggcat
     4201 gaattacctg ttctacatga gcatcgggaa cgccgtaggc tatattattg aaaatggtat
     4261 cgctgaacat catcggatcc tgctgtacaa atcctatgca tgaacggtag aactccatgt
     4321 caatatcctt tatgttgacg ccatctagca gtatatctcc ttcataatcg gaatagaacc
     4381 tcatgataag gttgaccagt gtggattttc ccgaacctgt agttcctacg attccaacca
     4441 cgtctccagg ctcaatcaca agatttatat cagacagcac tttcttggtg cggtcaaagg
     4501 agaagtttac atttctaaac tcaatcttgc cttttacttc tccaagaact ctgcccttgc
     4561 catagtcagg ttcaggttct gcatctatta tatcaagtat tctttcaatg gatgtaaggg
     4621 cgctctggta aacgtcgctg aagtttgcaa agaaacctac aggaccataa aacattgcag
     4681 tatatgatat gaaggaaaca agaagaccca ctgaaaggct tcctgaaata acccatttgc
     4741 ctccaatgct ccagataagt aatgaaccca atgtgacaaa cagagcaact gcattaggga
     4801 aggtgttcgt tatttttgct gagtttgtat ccacctttct ccactcagca gtgtatcttt
     4861 caaacttctt tacagcactc tgctcttttg caaaggactt gaccaccctg atgttgggta
     4921 ttgtatcagt aagtacagag gaaacagcag cgccgcgcct ccatattcta atgtagtaag
     4981 gcctaatctt ttcagcaaat acctttgcac caattacaac aaaaggtaca gggatcagag
     5041 acaggagtgt aagtttccag tgcattgtaa gcattatgac tactatacca ataagcagca
     5101 tgaactgcac tagtatttcc tgtgtaaccc tgatgataaa acccctaata ttctcagtat
     5161 cgccatttat tctgtttatt acggaaccgg ttgtggtttt gtcgtaaaac ttcatgggca
     5221 gatactgggc tttttgatat acatcattcc ttaaggactc aacaatcctg tttccgctca
     5281 tcctgagtag gtagcctctg tatgctgcta ctgagtagtg gactatgtac gaaacaagca
     5341 gaacaattac aacgattgta agagctctgt agtccttgtt tggcaaaatg ctatttaata
     5401 gctcctttgt gatatatgga gggagcagtg caattgccgt tgttataacg gacaaaaaaa
     5461 ggctgaacag aaggggccac ttttcaggga ttatgtattt tataaacttg gttgcgatct
     5521 tctttttgga ctggcacttt atacagtcgg cgcctggcct tatgagggtc cttccgcagg
     5581 ttgggcagac actcattagt ttttcaaagg ttgcttcggc aatttcaagc tcgtccttta
     5641 atggagagcc cttgcctatt tgaactataa aggatgaagc catctcacac aacgttgtta
     5701 cagtatatgt aaatcggaag aacctcagta ttcttccgtc gttgagctca atattgagct
     5761 gggcattgcc gtacatcctt ttgatggagg catcctttat ctctttatat tcatatgtga
     5821 gtattccatc tgcatgagaa ctatccactg acatcacctt cttgtcggta actgccagcg
     5881 ctgattctcc atacttgtcc tccagggtca agtctccaat aattacaaac aataatcttt
     5941 cgtcatcaac caattttgct tcaacaagct tcctgatagt ctcaggaatg ggcttgtttt
     6001 ttagtgcatt ttcatattcc accatttaca cctccaagat ggagaaaata ttaaattgac
     6061 aaagtaatta tagccttgat gtaattaatg
//
LOCUS       DAONVY010000068         6078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9703_length_6078_cov_3.545077, whole genome shotgun sequence.
ACCESSION   DAONVY010000068 DAONVY010000000
VERSION     DAONVY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9703_length_6078_cov_3.545077"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLI11_02955"
     CDS             complement(<1..197)
                     /locus_tag="PLI11_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51854.1"
                     /translation="MKKIGRRARELYASYNTVIEESLVQNINRIYYLSIISMIMSLID
                     VLLIKFGNSGEESPITWEVGI"
     gene            525..923
                     /locus_tag="PLI11_02960"
     CDS             525..923
                     /locus_tag="PLI11_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HPZ51855.1"
                     /translation="MLGLELIRKNIFEDPFFSDFFRFEECDSELKVDIVDNEKDYTLY
                     ADIPDVDKKDVDLKISNDGVLIIEVNQKEDNGRKFLRKERTVRKLKRSFAFDGIKTDG
                     VEASVENGVLTAKLPKVDISVNNERNIEIK"
     gene            973..1392
                     /locus_tag="PLI11_02965"
     CDS             973..1392
                     /locus_tag="PLI11_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPZ51856.1"
                     /translation="MSLIPFRNHRLTNFEPFFDSFMKPFFSDGFFSNDMRIDIAENEK
                     EYVLKAELPGVDQKDIDILVRDNVLTISAKRNEEKNEQRGNYIRKERFSGTVARSFTL
                     DGIDDSNIEASFDKGILTLTLPKHEESNPSFRRIELK"
     gene            complement(1419..2225)
                     /locus_tag="PLI11_02970"
     CDS             complement(1419..2225)
                     /locus_tag="PLI11_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPZ51857.1"
                     /translation="MLELLMKTNAFKVSSGNSPFWYTSGKIGPFYINTHYLFGSEESA
                     GKLLTDIDSLKNDRRSLIKMLEDRVKKQYEENGVYRKTIDTLVGLIKKNVDVDGISYV
                     SGGERRDWFFSIMCAFLLNKKHIYLFKDNTSFPAVNGGKCLHVADLITEGSSYERSWI
                     PVLEGMGIQMTDTVVCVNRNQGGNALLREKGVNVHSLVSVDGYMMRELYEKRLVDEKQ
                     YHMILEYIEDPDDSMKRFIKNNPDFLDKALNSDERTRQRALLCIEKGYYN"
     gene            complement(2240..3136)
                     /locus_tag="PLI11_02975"
     CDS             complement(2240..3136)
                     /locus_tag="PLI11_02975"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPZ51858.1"
                     /translation="MINVGGLELKNPVIAASGTFGFGREFSEYYDLGRLGGISVKGLT
                     LEPRMGNKTPRICETPSGILNSVGLQNPGVHEFVRTEIPFLRQYDTKIIANIAGNSFS
                     DYEQMAEILNEADVDAIELNVSCPNVKEGVVFGQEPTLLEKLTRSVKDRFDKPLFVKL
                     TPNVTSIVDTAKAAEAGGADGVSLINTLLGMAIDIYKRRPILANNTGGLSGPCVKPVA
                     VRMVHEVYRNVNIPVIGMGGIETGEDAVEFIIAGASCVMVGTASFVDPLACIKVIDGI
                     EEYMKKANVKDICELVGCLELN"
     gene            complement(3129..3890)
                     /locus_tag="PLI11_02980"
     CDS             complement(3129..3890)
                     /locus_tag="PLI11_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPZ51859.1"
                     /translation="MKTNYLCRVEELKRLTDTIWLLELEASNIREAIKPGQFFHVKCD
                     DGYLRRPFSVCDFKEGILKIAFEARGKGTKALSKVERGDNIDVLGPLGNGFDIGYDRI
                     CVVGGGIGVFPLLYLLNKSESLRKDAVLGFLCKDKVVLENEFANLCDNLVIACDDGSY
                     GVRGFVTTALEKHLEKHDFDIVYTCGPSIMMKKVYEICKKHNIPCQVSLEERMGCGIG
                     ACLVCVCEVKKDKVLQNKRVCKDGPVMWANEVYYD"
     gene            complement(3887..4804)
                     /gene="pyrF"
                     /locus_tag="PLI11_02985"
     CDS             complement(3887..4804)
                     /gene="pyrF"
                     /locus_tag="PLI11_02985"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPZ51860.1"
                     /translation="MKNFADKLIEEIKNKESYVVCGLDPIWESIPEGVKVRFKSKGAA
                     IFAFNKAIIDAIHDVVVCVKPQLAYYEQYGINGLIAFKATVEYARKKGLLVIADGKRN
                     DIGSTSLAYAKAYLSKGDASDLAFDCDALTVNPYLGWNGIQPFVEMCKQNSKGIFILV
                     KTSNPSSGELQDLIVEGGRTIYEKVAELVDKWGSELVGDSGYSSVGAVVGATYPEQAE
                     RIRKIIPRSIILVPGYGAQGGNAKSVVVNFNDDGLGAVVNASRSILYAYKSDRWSGAY
                     DEDAYADAARAEAVYMRDEINMELEKRQK"
     gene            complement(4809..6032)
                     /locus_tag="PLI11_02990"
     CDS             complement(4809..6032)
                     /locus_tag="PLI11_02990"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPZ51861.1"
                     /translation="MVLKNIRYVSVNEDKVMDIHLDDGSDKIVDAKNLVAIPGLVDMH
                     CHLRDPGYEYKEDIASGVKAAAKGGFVKIACMPNTKPVIDNSAVVSYIRKKAESLKSV
                     KVYPIGAITKNIEGKELAEIGDMKAHGAVAISDDGNPIMSSSLMKKAMIYAHSFDMPV
                     ISHCEDKDLAEDGSMNEGFVSTVLGLRGIPKAAEEAMVAREIILAEYTGLPVHIAHVS
                     TRLAVHMIRDAKKRGVKVTCETCPHYFTLTDEAVYGFNTYAKVNPPLREKEDVKAIIE
                     GLKDGTIDAIATDHAPHHEDEKKIEFQLAANGISGFETAFGLSFTYLVKAGHLSLGEL
                     VEKMSIKPLEILKIEDEGLNLTIFDPDEKYVVEPERFVSKGKNSPFGGFELFGEVMYT
                     VVDGRIVYTSYKVKG"
BASE COUNT         1818 a         1470 c          983 g         1807 t
ORIGIN      
        1 atgattccta cttcccaggt tattggtgat tcctctcccg aattaccaaa cttaatcaac
       61 aaaacatcaa tcagactcat gatcatggag attatcgaaa gataataaat tctgttaata
      121 ttctgtacca ggctttcttc aattaccgtg ttgtatgatg cgtacaattc tctagctctt
      181 ctaccaattt tcttcatcta tatcaccatt tttcttttat ctaattagct catattacca
      241 tatagaaata ataatgtcat tagattatta gtgctaagag ggagcatgcc ccgcaaacaa
      301 gaatataata aaccatatgc atttgttata atcaataagt caagaaattt agcaaaacaa
      361 agatatttaa agcaagacag aggaaaacaa agatatatga cagcattgag ctttaaaggt
      421 caaatatggt caaattttaa acttgcgtta gtaagaatga cctactataa taaagtcaga
      481 ccaaaggtca aagaaagtca aagacaaata ggaggtaata aagaatgtta ggtttggaat
      541 taatccgtaa gaatatattt gaagacccct tcttctcaga cttcttcaga tttgaggaat
      601 gcgacagtga attgaaggtg gacatagttg acaatgaaaa agattatacc ttgtacgccg
      661 acatacctga cgttgacaag aaagatgtgg accttaagat ctccaatgat ggagtactca
      721 taattgaagt aaatcagaag gaagacaacg gcagaaagtt tcttagaaag gaacggactg
      781 tcagaaaact gaaaagaagt tttgcatttg atggaatcaa aactgatggt gtggaagcat
      841 cagtggagaa tggagtctta actgccaaac ttcccaaagt tgatattagc gtcaacaatg
      901 agagaaacat agaaataaaa tagcgagata taatatgaat aataaacaaa agatttaaag
      961 gaggaatgca ttatgagttt gatacctttc agaaaccata gattgacaaa ttttgagcca
     1021 ttctttgatt catttatgaa acctttcttt agcgatggat tcttctcaaa tgacatgaga
     1081 atcgacatcg cagagaatga gaaagagtat gttttgaaag ctgaattgcc aggagttgac
     1141 cagaaagaca tagacatcct cgtcagggac aacgtcttga caatatcagc caagagaaat
     1201 gaggaaaaaa acgaacagcg cggcaattac attagaaagg aaagattctc aggaaccgta
     1261 gcaagaagct ttaccctgga tggcatcgac gattcaaata tcgaagcttc ctttgacaag
     1321 ggaatactta cccttacact gccaaaacac gaagaaagca atccatcatt tagacgaatt
     1381 gaacttaaat aaagcggcgg ccagaaatgg ccgcttcttt agttataata tcctttttca
     1441 atacacaaca aagccctttg tcttgttctt tcatcactgt ttaaagcttt gtcaagaaaa
     1501 tcaggattgt ttttaataaa tcttttcatt gaatcatccg gatcttcaat gtattcaaga
     1561 atcatatggt actgtttttc gtcaacaagc cttttctcat ataactctct catcatatat
     1621 ccatccacac taacaagcga atgaacattt acaccctttt cccttaacag agcgttgccg
     1681 ccttgatttc tatttacaca aacaaccgtg tccgtcatct gtatacccat cccttccaga
     1741 acaggaatcc aggacctttc atagcttgaa ccttccgtaa tcaaatccgc cacatgcaga
     1801 cactttcccc cattaacagc aggaaaggac gtgttatcct tgaaaagata aatatgtttc
     1861 ttgtttaata gaaaagcaca cattattgaa aagaaccagt ctcttctttc tccacctgaa
     1921 acatatgata tgccatccac atccacattc ttcttaatta aaccaactag agtatcaatg
     1981 gttttcctat aaactccatt ttcttcatac tgctttttaa ccctgtcctc aagcattttg
     2041 attaaagatc ttctgtcatt ctttaaagaa tcaatatcag tcagtagctt tccggcactt
     2101 tcttcgcttc caaacaaata atgggtgttg atataaaatg ggccaatctt tcctgacgtg
     2161 taccaaaaag ggctgttccc agacgaaacc ttaaaagcat tcgttttcat caaaagttca
     2221 agcatacaaa tctccttcct taattaagct ctaggcaacc aacaagctcg cagatatcct
     2281 tgacatttgc cttcttcatg tattcttcaa ttccatcaat aactttaatg catgcaagag
     2341 gatcaacaaa actagcagta ccgaccatta cacaggaagc ccccgcaatt ataaattcca
     2401 ctgcatcctc tccagtttca attccaccca ttcctataac aggaatattg acattcctgt
     2461 atacctcatg caccatcctt actgcaacgg gctttacaca gggacccgac aatccacctg
     2521 tgttgttggc aagaataggt cttcttttgt aaatgtcaat tgccatgccc aaaagagtat
     2581 ttataaggct aacaccatcg gcaccgcctg cttcagcggc cttagctgtg tcaacaatag
     2641 aagttacatt cggggttaac tttacaaaca acggcttatc aaatctatcc tttacacttc
     2701 ttgtaagttt ttcaagaagt gtcggttcct gcccaaatac caccccttcc ttgacattgg
     2761 gacaggatac atttagctcg attgcatcca catctgcctc attcaatatc tccgccatct
     2821 gctcatagtc tgaaaaagaa tttccggcaa tatttgcaat aatttttgta tcatactgtc
     2881 ttagaaaagg tatttccgtc cttacaaact catgtacacc cggattctga agtcccacac
     2941 tattaagtat gcctgaaggc gtttcgcata tcctgggggt tttgttcccc attctaggct
     3001 ccagggtaag accttttact gaaatgcctc caagacggcc aagatcataa tattctgaaa
     3061 attcccttcc aaatccaaaa gtaccggatg cagcaataac cggatttttt agttcaagtc
     3121 caccaacatt aatcatagta cacctcattt gcccacatta caggtccatc cttgcaaacc
     3181 ctcttgtttt gcagaacctt gtccttcttt acttcacata cacatacaag acatgcgcca
     3241 ataccgcagc ccattctctc ttccaaagaa acctgacaag gaatattatg tttcttgcat
     3301 atctcataca cctttttcat cattatcgaa ggaccgcagg tatacacgat atcaaaatcg
     3361 tgtttttcaa gatgcttttc aagggcagtg gttacaaaac ctcttacacc ataacttcca
     3421 tcgtcacaag caatgacaag attgtcacag agattcgcaa attcattttc aagcaccacc
     3481 ttgtccttgc acaaaaagcc caataccgca tcttttctta agctttcact tttattcaaa
     3541 agatacagaa gtggaaaaac tccaattcct ccacctacaa cacaaattct gtcatatcca
     3601 atatcaaagc cgtttccaag aggtcccaaa acatctatat tgtctccacg ttcaaccttt
     3661 gaaagtgcct ttgtaccttt gccccttgcc tcaaatgcaa ttttaagaat accctctttg
     3721 aagtcacata cgctaaaggg tcttctgaga taaccatcgt cacatttgac atggaaaaac
     3781 tgtcccggct ttattgcctc ccttatgttg gatgcttcca gttccaaaag ccatatggta
     3841 tctgttagtc ttttcagttc ttcaactctg cacaaataat ttgttttcat ttctgcctct
     3901 tttccagttc catatttatc tcatccctca tgtataccgc ctctgccctg gcagcgtcag
     3961 catacgcatc ttcgtcatac gctccactcc atctgtcgga tttgtatgcg taaagtatgc
     4021 ttcttgaggc atttacaacc gctcccaggc cgtcgtcatt aaagttgact acaacactct
     4081 ttgcatttcc tccctgtgct ccatagccag gtacaagaat aatactgcga ggaataatct
     4141 ttcttattct ctccgcctgt tcgggatatg ttgcccccac aacagcacca acagaagagt
     4201 aaccactgtc ccctaccaac tcgctgcccc acttgtcaac aagctccgcc accttctcat
     4261 atattgtcct gccaccttcc acaatcagat cctgcagctc tccagaagaa ggattggagg
     4321 tcttcaccag gatgaatata cctttgctat tttgtttgca catttcaaca aaaggttgta
     4381 tgccgttcca acccaggtat gggtttacgg tcagcgcatc acagtcaaag gccaggtccg
     4441 aagcatcacc ttttgaaaga taagcttttg cgtaagccag cgaagtggaa cctatgtcat
     4501 tccgcttgcc atctgcaata acaagcagcc ccttcttgcg agcatactca accgttgcct
     4561 tgaaagctat aaggccgtta ataccatact gctcatagta tgccagctgt ggtttcacac
     4621 aaacaaccac gtcatgaatc gcatctataa ttgccttgtt gaaagcaaag attgcagcac
     4681 ctttgctttt gaacctaact tttacccctt ccgggatact ttcccaaatt gggtctagtc
     4741 cgcatacaac ataactctcc ttgtttttta tctcttcaat aagtttgtcg gcaaaatttt
     4801 tcatttcatc accctttaac cttgtagcta gtataaacta ttcgtccatc cacaacagta
     4861 tacatgacct ctccaaacag ttcaaatcca ccaaaaggag agtttttacc ctttgacaca
     4921 aacctttcag gctccaccac gtatttttcg tccgggtcga agattgttag attcaaacct
     4981 tcatcttcga tctttaatat ctcaaggggc tttatactca tcttttcaac aagctcacca
     5041 agactcaggt gtcctgcctt gaccagataa gtaaaagaaa gaccaaacgc agtttcaaaa
     5101 cccgatattc catttgcagc aagctggaac tctatcttct tctcatcctc gtgatgaggt
     5161 gcatggtctg ttgcaatagc atctatcgta ccatccttca gaccttcaat aattgctttc
     5221 acatcctctt tttccctgag aggaggattg acttttgcat acgtgttgaa tccataaact
     5281 gcctcatcag taagagtaaa atagtggggg caggtttcac aggtaacctt tacacctctt
     5341 tttttggcat cccttatcat atgaactgca agacgcgtgc tgacatgcgc tatgtgtacg
     5401 ggcagacccg tatactccgc cagaatgatt tctcgtgcca ccattgcctc ttcagcagcc
     5461 tttggtatac ctctcaaccc caataccgta gagacaaacc cttcattcat gcttccgtct
     5521 tccgcaagat ccttgtcctc acagtgagaa atgacaggca tgtcaaaact gtgggcgtaa
     5581 atcatggcct tcttcatcaa tgacgaagac attataggat tgccgtcatc actgattgca
     5641 accgccccgt gagctttcat atcccctatt tcagccagct ccttgccttc tatgttcttt
     5701 gtaattgctc caattggata cactttcacc gacttgaggc tttctgcctt tttcctgata
     5761 tatgaaacta cagctgaatt atcaattacc ggttttgtgt tcggcatgca tgcaatcttt
     5821 acaaatccac cttttgcagc tgctttgaca ccacttgcaa tgtcttcctt gtactcatag
     5881 cccggatccc ttaaatggca gtgcatgtcc acaagcccgg gtatcgccac aagatttttg
     5941 gcatccacaa ttttgtcgct gccgtcatca agatgtatgt ccattacctt gtcttcattc
     6001 acagatacat atcgaatatt ctttaatacc atgtcacacc tctttatcta gtaaggatat
     6061 acaaaattgc cattctaa
//
LOCUS       DAONVY010000069         6076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9708_length_6076_cov_3.667995, whole genome shotgun sequence.
ACCESSION   DAONVY010000069 DAONVY010000000
VERSION     DAONVY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9708_length_6076_cov_3.667995"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLI11_02995"
     CDS             complement(<1..329)
                     /locus_tag="PLI11_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPZ51862.1"
                     /translation="MKDIYEISMLIDLYGQLLTPSQLKCLELHHNHDLSLAEIAEEMK
                     ISRQGVHDFIKRGKAALYEYEEKLGLLERFLNVKKQLESIQYDFAFLNDEELGDDNRN
                     ILSSIET"
     gene            complement(420..1331)
                     /gene="ftsY"
                     /locus_tag="PLI11_03000"
     CDS             complement(420..1331)
                     /gene="ftsY"
                     /locus_tag="PLI11_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPZ51863.1"
                     /translation="MSFFDKLKSGLKKTKENFMGKIDAVLASFGKVDEELFESLEDVL
                     ISSDFGVPTTEKIMDSLRQRVKADRVTDSEVVKSYLKEEIAKLLEGDSAKGLDLDATP
                     VVITVIGVNGVGKTTSIGKMANLYKNAGKKVLLAAGDTFRAAAIDQLVIWAERADVDI
                     VKQKEGSDPSSVLFDACQAAKARNVDVLICDTAGRLHNKKNLMDELAKMARVVDKEMP
                     HARKEVLLVLDSTTGQNAVNQAKAFKDVANITGIILTKLDGTSKGGIIVSICSDLGIP
                     VRLVGVGEGIDDLQTFNAKDYVKALFE"
     gene            complement(1328..4906)
                     /gene="smc"
                     /locus_tag="PLI11_03005"
     CDS             complement(1328..4906)
                     /gene="smc"
                     /locus_tag="PLI11_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPZ51864.1"
                     /translation="MYLKRLEIQGFKSFVDKTVLEFDKGITAIVGPNGSGKSNISDAV
                     KWVLGEQSPKTLRGGKMEDVIFAGTENRRPVGMAEVSLIMDNSDRTLNIDYSEVKITR
                     RLYRSGESEYLINGSLCRLKDIQLLFADTGIGKDGFSIIGQGKVDEILNSKSNDRRGI
                     FEDASGIMKYRMRKEESERKLNNTEQNLVRINDILGELERQIGPLEKQAEKARKYLTL
                     KYELRDIEVGDLVDTIKDSSVKMEETDKNIFIVEDQIKQTEEKLENVKRENQEKTEKS
                     KALEEQIYREREKGHEIGNLIERINSSIKINENNINTLKQAVEKNEMELSDLSNRNIK
                     LQNDVANVDERILVTEEQIEEARQKISDMQERYNAVMSLLSDDSREAEKLKDWLYELR
                     LRLSQKDSEKESCRSNIERLDKRNIEIRDEIENANKEHLEIDDLIKKAEEEYIQSASL
                     AESFKKEWNENADKLNGMKVLYENKTNSLSDTAAEMKSQQARLRLMSEMQEKYEGYAR
                     SVKEILTFCRQNASFGRGIHGSVAEIINVPAEYELAVESAIGAAYQNIVTDTEDEAKK
                     AIDYLKQKNYGRATFLPISAVQGRTIDSNTERQLKTMPGFVGIAANLVRYDGKFGNIV
                     NNLLGRVVIFTDLDSCINAARRFKYSFVCVTLGGDILRTSGAMTGGSTNNNNKTNVFA
                     RTREIPALERSVKQLSSSLDSLKHEIVGLRENINRLEKEQNSILDKIRKQEIQTERAK
                     NVYNALVEKKGNLDKKVEDLMRDADRNVNEISVLKERTNAIDVEIVDINNQIMEKSKE
                     LAQYEEKSKQGAEVRNVLTNELTKAQVEENEIKNRLNNLFDEKKRLLNEIASNEDKKN
                     YLKLQIDEDSSRIKELVIENEEYASRIKKQVHDKDDNSALLARLTVEKSEIDANLSGM
                     IEKISEITGEISNLREELSRLAFRKTKLEETKESCLNRLWDEYELTYTKACEISSFSG
                     KVNKEARRKRINELKQEMKELGNVNVGAIDELEEVKTRYEFMSEQYNDMHTAKQKLLE
                     VIDEMNKVMKEQFTKQFDQIRRNFNEVFCELFGGGKADIILTDEANVLESGIEIEVQP
                     PGKKLQNMLLLSGGERALTAIALLFAILRMRPSPFCLLDEIEASLDEANVYRFGRFLL
                     RGSDKTQFIMVTHRKGTMEIAETMYGVTMEEKGISKVVSMKLGGVK"
     gene            5029..5322
                     /locus_tag="PLI11_03010"
     CDS             5029..5322
                     /locus_tag="PLI11_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HPZ51865.1"
                     /translation="MFGNRKRFNKIQKQLRMLNLRMERIKFLDYIYYKENPYKLLWSN
                     FLSGLARGLGGAIGFSIISAIIILILQWVVRQNLPYISDFIADILNAVNSKCK"
     gene            complement(5363..>6076)
                     /locus_tag="PLI11_03015"
     CDS             complement(5363..>6076)
                     /locus_tag="PLI11_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPZ51866.1"
                     /translation="NLLHKVNESFDEYEFHALFHQIHNFCVVDMSNFYLDIIKDRLYT
                     SRPESSQRRSAQSAMFIILDTLVRILTPVLAFTSEEIWQHMPHKEGDETLSIQLNYWP
                     ELTERYKDNTLEEKWDKLIDIKYDVAKVLELARNEKKIGHSLDARVVVYADGEEFSFI
                     KENEDLLNTVCITSAFEVRKLGEAPENAADSEYFENIKLIVEDAPGHKCERCWMYSET
                     VGSVEEHPSICSRCYSNIK"
BASE COUNT         1522 a         1512 c          964 g         2078 t
ORIGIN      
        1 ttggtctcaa tacttgatag tatgttccgg ttgtcgtcac ccagttcctc gtcgtttaag
       61 aatgcaaagt catactgaat tgattccagc tgttttttta cattcagaaa cctctccaaa
      121 agtcccagtt tttcctcata ctcatagagt gcagcttttc cccgtttaat aaaatcgtgc
      181 actccctgcc tgcttatctt catctcttcg gcaatctctg ctaggctgag atcatgattg
      241 tgatgcagct caagacactt aagctggctt ggtgtcagaa gctggccata aaggtcaatc
      301 agcatgctaa tctcataaat atctttcaca agcaaccacc ttccctcgcc cagttcaacg
      361 ctgtaaagct tgaactcttt acaatataca gtataagaat ctatttgtca agaaaaaaat
      421 cattcaaaaa gggcttttac ataatctttt gcgttaaatg tctgaaggtc atctatacct
      481 tctccaactc caaccagtct tacagggata cccaaatcgg aacatatgga aactatgatt
      541 cctccctttg aagtaccatc cagttttgtc aaaataatac ctgtaatatt ggcaacatcc
      601 ttgaaagcct ttgcctggtt gacagcattc tgtcctgttg tggaatccag tacaagtaat
      661 acctcttttc ttgcatgggg catttccttg tcaacaactc ttgccatttt tgcaagttca
      721 tccataaggt tctttttgtt atgcagtctg cctgctgtat cacaaatcag tacatctaca
      781 ttccttgctt ttgctgcttg gcaagcatca aacaaaacgc ttgacgggtc agagccttcc
      841 ttctgcttta caatatcgac atctgccctc tcagcccaga tgacaagctg gtcaattgcc
      901 gctgcccgga aggtatctcc tgcagcaagc aaaacctttt tacccgcgtt cttgtacaaa
      961 tttgccattt ttcctatgga tgtagtcttt cctactccgt taacaccaat aacagttatg
     1021 acaacaggag ttgcatcaag gtcaagacct ttggcactgt ccccttcaag aagcttggca
     1081 atctcttcct tcaggtatga tttcacaact tcagaatcag taaccctgtc agccttgact
     1141 ctttgcctta gactgtccat aatcttctca gtcgttggca caccaaaatc agaacttatt
     1201 aaaacatctt ccaaagattc aaacagctct tcatcaactt ttccaaaaga cgccaatacg
     1261 gcatctattt ttcccataaa gttttctttt gtttttttaa gcccactctt cagcttgtca
     1321 aaaaaactca ttttactccc cctaatttca tagatactac cttggatatt cccttctcct
     1381 ccatggtcac accatacatg gtttcagcaa tttccatggt tccttttctg tgagtcacca
     1441 taataaactg ggttttgtct gaacccctta gcaaaaatct gccgaacctg tatacgtttg
     1501 cctcgtccag tgaagcttct atctcgtcaa gtagacagaa aggcgaaggc ctcattctaa
     1561 gaatggcaaa taacaaagca atggcggtaa gcgccctttc tccaccagac aggagcagca
     1621 tgttctgcag ctttttacct ggaggctgta cttctatttc tattccgctt tcaagcacat
     1681 tagcctcatc cgtcaatatt atatccgctt ttcctcctcc gaacaactcg cagaacactt
     1741 cattaaaatt ccgccttatc tgatcgaact gttttgtaaa ctgttccttc atcaccttgt
     1801 tcatctcatc aatcacttca agaagcttct gcttggctgt atgcatgtcg ttgtactgct
     1861 cgctcatgaa ctcatacctt gtcttaacct cttccagttc atcaattgcg ccgacattca
     1921 cattgccaag ctccttcatc tcctgcttga gttcattgat tcgtttcctg cgggcctctt
     1981 tgttgacctt gccagaaaaa ctgcttatct cacatgcttt tgtgtatgta agctcatact
     2041 cgtcccacaa cctgttcaaa caactctcct tggtctcctc aagttttgtc tttctaaatg
     2101 cgaggcggga aagctcttct ctaagatttg agatttctcc tgttatctca ctaatcttct
     2161 caatcatgcc ggaaagattt gcatcaatct cgctcttttc gacagtaagc cttgccaaaa
     2221 gtgcagaatt gtcatccttg tcatgtacct gcttcttgat ccttgaggca tactcttcgt
     2281 tctctattac aagctccttg attctgctgc tgtcttcatc aatctgcagc ttcaaatagt
     2341 ttttcttatc ctcatttgaa gcaatctcat ttaatagtct tttcttttcg tcaaacagat
     2401 tattcagtct gttctttatt tcattttctt caacctgtgc ttttgtaagc tcgttggtaa
     2461 gaacatttct aacttcagca ccttgttttg acttctcttc gtactgggca agctccttgc
     2521 ttttttccat aatctgattg tttatatcaa caatctccac atcaatagca ttagttcttt
     2581 ccttaaggac ggagatttca tttacattcc tgtccgcatc ccgcatcaag tcctcaacct
     2641 tcttgtcaag gtttcctttc ttctccacta aagcgttgta gacgttctta gccctctctg
     2701 tttgaatttc ctgttttctt atcttgtcaa gaatgctgtt ttgttctttt tccaacctgt
     2761 ttatattctc cctcaaacca actatttcat gttttaggga atccaaagag cttgaaagtt
     2821 gtttgacaga cctttccaga gcaggtattt cccttgttct ggcaaaaaca tttgtcttgt
     2881 tattgttatt tgtgcttcct cctgtcatcg caccggaggt tcttaatata tctccaccaa
     2941 gagtcacaca aacaaaagaa tatttaaacc tcctggcagc gttaatacag ctgtcaaggt
     3001 ctgtgaaaat tacaaccctg ccaagaagat tgttaacgat gttcccaaac ttcccatcgt
     3061 accttaccag atttgctgca ataccaacaa atcctggcat ggttttaagc tgtctttccg
     3121 tattggaatc tatggttctt ccctgaacag ctgatatggg aagaaaagtt gctcgtccat
     3181 agtttttctg tttcagataa tctatggcct tcttcgcctc gtcttccgta tcggtcacta
     3241 tattctgata tgctgcgcca atagcacttt ccaccgccag ttcatactct gccggcacat
     3301 tgataatttc cgcaacagaa ccatgaatac ctcttccaaa ggaggcgttc tgcctgcaga
     3361 aagtgaggat ttcctttacg cttcttgcat agccctcgta tttttcctgc atttcactca
     3421 tcagtctgag tcttgcctgc tgtgatttca tctccgcagc agtatcggac agactgtttg
     3481 ttttattttc ataaagtacc ttcataccat tgagcttgtc agcattttca ttccattcct
     3541 ttttaaagct ctcggccaaa gacgcacttt gtatgtattc ctcttcagct tttttaataa
     3601 ggtcgtcaat ttccaaatgc tccttgtttg cattctcaat ttcatccctt atttctatat
     3661 tacgcttgtc aagcctttca atgttgcttc tgcaggattc cttttcagaa tctttctggg
     3721 acagcctgag tcttaactca taaagccaat ccttgagttt ttccgcctcc ctgctgtcat
     3781 cactaagaag tgacattacc gcattgtatc tctcctgcat gtcactaatt ttctgccttg
     3841 cttcttctat ctgttcttca gtaacgagaa ttctttcatc tacatttgca acatcattct
     3901 gaagcttgat gttcctgttg gaaaggtcgg aaagttccat ttcatttttc tcaacagctt
     3961 gcttcagagt atttatattg ttttcattta tctttattga tgaatttatt ctttcaatga
     4021 ggttgccaat ctcatgacct ttttccctct ccctataaat ctgctcttca agggctttgc
     4081 tcttttctgt tttctcctgg ttttctcttt ttacattctc cagtttttct tccgtctgct
     4141 ttatctggtc ctcaacgata aatatgttct tatccgtttc ttccattttc acactggaat
     4201 ccttgattgt gtccacaagg tcgccaactt caatatccct taattcatat ttaagggtca
     4261 gatatttcct ggccttctct gcctgctttt caagaggccc aatctgcctt tccaactcac
     4321 caagaatatc atttattctg acaagattct gctcggtgtt attaagtttt ctttcgcttt
     4381 cttcctttcg catcctgtac ttcatgatgc ctgaagcgtc ttcaaaaatg cctcttctat
     4441 cattggactt gctgtttaga atctcatcca ccttgccttg tccaataatg gaaaagccat
     4501 ccttgccaat acccgtatcg gcaaacaaaa gctgaatatc cttcaaacgg cacaatgagc
     4561 cattgataag atactcactc tcaccagagc ggtacaacct tctcgttatt ttaacttccg
     4621 aataatctat attcaaagtt ctgtcactgt tatccattat taaagacact tcggccatac
     4681 caacgggacg ccgattttca gtacctgcaa atatgacgtc ctccatcttg ccgcccctaa
     4741 gtgtttttgg gctctgctca ccaagaaccc atttgacagc gtcggaaata ttgctctttc
     4801 cactgccgtt tggtccaact atggcggtta tgcccttgtc aaattccaga acagtcttgt
     4861 ctacgaatga tttgaatcct tggatttcaa gtctttttag atacaagttt atcccccaaa
     4921 cctaatttta tccgctcaat tatatcataa atattagaac tatacatcct tttgaatatt
     4981 taaacttttc aaggttaaaa taaaatatat tcaagttgcg gaggtaacat gttcggtaat
     5041 agaaaaagat tcaataaaat acaaaaacaa ctgcgcatgc taaaccttag aatggagcgc
     5101 atcaaatttc ttgactacat ctattacaag gaaaatccat acaagctcct gtggtcaaac
     5161 tttctgagcg gtcttgccag aggccttgga ggagccatag gattctccat catttccgca
     5221 attatcatac tcatacttca gtgggtggtt agacaaaacc tcccctatat ctccgacttc
     5281 attgcagaca tattgaatgc agtaaactca aaatgcaaat gaaaccaaaa gagaccgaaa
     5341 tttctttcga tctcttttat agctatttaa tgttgctgta gcatcttgag caaatgctcg
     5401 gatgttcttc cacagaacca acagtttctg aatacatcca gcatctttcg catttatgac
     5461 cgggagcatc ctcgacgatg agttttatgt tctcaaaata ctctgaatca gctgcgtttt
     5521 ccggcgcttc tcccaattta cgaacctcaa atgcactggt tatgcataca gtgtttagaa
     5581 ggtcctcgtt ctcttttata aatgaaaact cctcaccatc ggcataaacc acaacccttg
     5641 catccaatga gtgacctatt ttcttttcat tacgtgcaag ttccaacacc tttgccacat
     5701 cgtacttaat atcaataagt ttatcccatt tctcttccaa agtattatcc ttgtatcttt
     5761 cagtaagttc aggccagtag tttaattgta tgctcaaggt ttcgtcgcct tccttatgtg
     5821 gcatgtgctg ccaaatctct tcacttgtaa aggcaagtac cggagtcagt atgcgtacaa
     5881 gagtgtcgag tatgatgaac atcgcacttt gtgcacttct tctctggctg ctttctggtc
     5941 ttgatgtata caatctatcc ttgatgatgt caaggtagaa gttactcatg tcaaccacgc
     6001 agaagttgtg aatctgatga aacagcgcat ggaactcgta ctcgtcaaaa gactcgttaa
     6061 ccttgtgaag gagatt
//
LOCUS       DAONVY010000070         6066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9727_length_6066_cov_3.590418, whole genome shotgun sequence.
ACCESSION   DAONVY010000070 DAONVY010000000
VERSION     DAONVY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9727_length_6066_cov_3.590418"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /locus_tag="PLI11_03020"
     CDS             complement(<1..1618)
                     /locus_tag="PLI11_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51867.1"
                     /translation="MDQKFMFSLLWPDYFEGKKMEDRIRSGDGKLYNQDFLERDMNVE
                     MLLEGMEVKNESRPYVKARLMDMVLDEKIIKYRQDILQDFLDYPEIQQAFDLELMPLV
                     INLNKMQKTNISHDDDIRKIAWRLEILRVYTQCVTILHRVFCSKDRDFTSEGLQRLQS
                     IISSIVENEGYKELVRLLPDLHEKLQTMSGVTLGVNLDHELRPTEAVLLSIEPKPFRN
                     KALLTSIFGTRRNDEMYHGIGTFYSILKDTTANALDQAIMRDLAYVMEDTFRHMAMVL
                     NRFAWVETAFIFDLVPEVYYYIGGSNLARNLLEGGMPVCFPTPMPAEDRVFIVKDLYD
                     ASFAIRILSDTGIKALHNIIVTNNARMCDDPGRLLILTGANQGGKTTFTRAVGIAQIL
                     FQAGLPIPGTEGKISPVDNIYTHFPELEKNSISEGRLGEECVRLEGILPRLTKYSMIL
                     MNESLSSTSHQESLTIAEEIMKYLRTIGARGVFATHMHELAEDLNEINKQSALSNLVS
                     LVAGVDEEQQMEVMTEEGIKTFRGSKRTYKI"
     gene            2017..2484
                     /gene="nrdR"
                     /locus_tag="PLI11_03025"
     CDS             2017..2484
                     /gene="nrdR"
                     /locus_tag="PLI11_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPZ51868.1"
                     /translation="MKCPYCNHLDDKVIDSRPTDEHTVIRRRRECLACGSRYTTYEKI
                     ESTQLFVIKKDGTRQAFDRYKLISGVMRACEKRPIKLKQVEALADEAEQYFLNSLKKE
                     ISSKEIGEFVMEKLRHLDQVAYVRFASVYKEFKDIDTFMEELKGIMQNSTRIE"
     gene            2580..3149
                     /locus_tag="PLI11_03030"
     CDS             2580..3149
                     /locus_tag="PLI11_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51869.1"
                     /translation="MYSLKRILEIALSGKHNCMLFGSVDLMPITCEEFFVSCYSPCKC
                     GLLFRRDCTCTDLDIQYTFANIPSRQLNSSDMYVPINDLRQDVDHERINTVHRIQEKR
                     YESNTFKFNGSLSRNGFEKYCRMDLEAQEFIKEAQKIYRCSMVDMDKVFRVSRTIADL
                     DLRETISKFDVAEALGYVSCYIYLKTTTI"
     gene            3168..4259
                     /gene="dprA"
                     /locus_tag="PLI11_03035"
     CDS             3168..4259
                     /gene="dprA"
                     /locus_tag="PLI11_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPZ51870.1"
                     /translation="MYSDNLVHIWITLLGRPANKIASLLVENYGSLREAYLVKSYTDV
                     TCEIRNNRLLCQRLMDESIKDRAARIYERVLEKGVKITHVFREDYPEDLKNIFDPPFV
                     LYYYGKLPDKNTTLLSVVGSRKCSAYGRKVTYDMCFNIAKHGVGIVSGMAKGIDARAH
                     VGCLDANGYTIAVLGGGPDNIYPLENEVIYYRIKENGLVLSEYPPGTMPLKHHFPARN
                     RIIAGLGKGLLVCEAGKKSGAMITVNFAINEGRDIFSVPGNIDSSYSEGCNSLIKAGA
                     CIATSFIDVLEGLNLIEGRKIAGTVGWLNGLDKEEAMVADLIYKGVKIEDICSKTGLD
                     SARVVAITTILEVKGILGRDRHGNLHVVI"
     gene            4335..>6066
                     /gene="topA"
                     /locus_tag="PLI11_03040"
     CDS             4335..>6066
                     /gene="topA"
                     /locus_tag="PLI11_03040"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPZ51871.1"
                     /translation="MADYLVIVESPTKANTIKKFLGRSYKVEASAGHVRDLPKSKLAI
                     DIENDFEPQYINIRGKGDLIKTLKKEAGKAKKVYLATDPDREGEAISWHLTQLLDIDA
                     NEPCRVTFNEITKNAVLEGFKNPRPIDMKLVDTYQARRVLDRIVGYKISPVLWKKVKK
                     GLSAGRVQSVATRLICDREDEIEAFEPKEYWSITALLSKFGKKDRFEAKFHGKQGGKI
                     EVENEEQAKTIYDEIENSEFKVVDLKKSERRRSPAPPFTTSTLQQEASRRFGFPASKT
                     MMVAQMLYEGIDLGGKQGVTGLVTYIRTDSVRISDNAQNQALEYIKGNFGDEYVPKKK
                     RVYKNKNQAQDAHEAIRPTYLEHAPGKIKDKLTNDQYKLYKLIYERFIASQMAEAVYD
                     TVSVDILTSKGDYLFKASGSTLKFKGYTAIYMDSKSDNNDENEEDGNRKLPKLEVGEV
                     LNLDKLDKKQHFTQPPPRYTEASLVKALEEHGIGRPSTYAPTISTILARGYVRREKKV
                     LFPTELGRIVNDLLKKSFNDIVNVEFTANIEDRFDKIAEGEENWVSVIRDFYTPFEKQ
                     VQKAEEELEKV"
BASE COUNT         1877 a         1057 c         1425 g         1707 t
ORIGIN      
        1 caatcttgta cgttctcttg gagcctctga atgtcttaat gccttcttcc gtcattacct
       61 ccatttgctg ttcttcatca acacctgcca caaggcttac taggttgctg agggcagact
      121 gtttgttaat ctcattcaaa tcttctgcaa gttcatgcat atgggttgca aatacgcctc
      181 ttgcaccgat ggttctgagg tatttcatga tttcctcagc aatagtcagg ctttcctggt
      241 ggctggtact tgacagtgat tcgttcatga gtatcatgct gtactttgta agtcttggca
      301 gaattccttc aagccttacg cactcttcac ccaaacggcc ttcgctgatg gagttcttct
      361 caagctcagg gaagtgagtg tatatattat ccacaggact gatttttcct tcggtgcctg
      421 gtattggaag accagcctgg aacagaatct gcgcaatgcc aactgctctt gtaaaggtgg
      481 tctttccgcc ctggttcgca ccagtaagta tgagcagtct tcccgggtcg tcgcacattc
      541 ttgcattgtt ggtgacgata atgttgtgaa gtgcctttat tccagtatcg gagagaattc
      601 gtatagcaaa gcttgcatcg taaagatcct ttactataaa tactctgtct tccgctggca
      661 ttggagtagg gaagcataca ggcattccgc cttccaagag gttgcgtgcc aggttactgc
      721 cgccgatgta gtagtagact tctggaacca aatcaaaaat gaatgcggtt tcaacccagg
      781 caaagcggtt gagtaccatg gccatgtgtc tgaaagtatc ttccatgaca taagccaggt
      841 ccctcattat cgcttggtca agagcattgg ctgtagtatc tttaagaatt gaataaaacg
      901 ttccaattcc atgatacatt tcatcatttc gtctagttcc aaaaattgac gttaagagag
      961 ccttgtttct aaaaggtttt ggttcaatgg acaacaaaac agcctcggtt ggtcttaatt
     1021 catggtccag attgacacca agagttacac cggacattgt ctgcagcttt tcatgcaaat
     1081 caggcaaaag tcttaccagt tccttatatc cttcgttttc tacaatgctg gaaataattg
     1141 attgaagtct ctgcagtcct tcagatgtga aatctctatc tttgctgcaa aagacacgat
     1201 gcagtatcgt gacacactgg gtgtacaccc tgagaatttc gagtcgccat gcaatttttc
     1261 tgatgtcgtc gtcatgggat atgttggttt tctgcatttt attcagattg ataacaagcg
     1321 gcatcagttc caggtcaaag gcttgttgga tttccggata gtcaagaaaa tcctgaagga
     1381 tatcttgtcg atacttgata attttctcgt cgagaaccat gtccatcaag cgggctttaa
     1441 cgtagggtct gctttcattt tttacttcca tgccttctag tagcatttcc acgttcatgt
     1501 ctctttctag aaaatcctga ttatacagtt tgccatcacc acttcttatc ctgtcttcca
     1561 ttttcttacc ttcgaagtag tcaggccaca agagactgaa cataaacttt tgatccattc
     1621 gtttctcact cctgtttgac ttttttactt taaaccatta tatcatgtga catattcaaa
     1681 atcaatgaag cagaccgtgc ataaaagatt aataaaccgt taccaaaaaa agaaatttat
     1741 ttttgtaatt tacagatact gtaacattaa gtaaaataag gataaaagga ataacgaaat
     1801 taaatacaga aatggagtgt attgttgtgt tatttaaaaa agcgtcagta ttctttgttt
     1861 tgttgttaac ttcataatcc cgcttgattg aataaatgaa aagggtttga tttgatgtct
     1921 tccggtttcc ttaactggaa gactttttat tccttgcatt gatataggaa aatgttgtat
     1981 aatatactaa atattttgaa agtgggtgga gctgacgtga agtgtcctta ctgcaatcat
     2041 ttggacgaca aggtaataga ctcaaggcct acggatgaac acaccgttat acgcaggcgt
     2101 agagagtgtt tggcttgcgg atccagatat actacttatg agaagattga gagcacccag
     2161 ttgttcgtga ttaaaaaaga cggaactaga caggcctttg acaggtacaa gcttatctct
     2221 ggtgtaatga gagcctgtga gaaaagacca attaagctca agcaggtgga agcgcttgca
     2281 gatgaagcag aacagtattt tttgaactct ttgaagaagg aaatttcctc caaggaaatt
     2341 ggagaatttg taatggaaaa gcttaggcat cttgatcagg tggcttatgt aaggtttgca
     2401 tccgtataca aggagttcaa ggatatagat actttcatgg aagaactaaa aggaattatg
     2461 cagaatagca caagaattga ataaataaga tctaaagctg aatatgattt tcatcccccc
     2521 agaatattgc aggaatgctg caatgatgga gggtttttta ttttcagaaa ggatatgtca
     2581 tgtatagttt gaagaggatt ttggaaatag ctttgtcagg aaaacacaac tgcatgcttt
     2641 ttggcagtgt tgatttaatg ccaatcacat gtgaagagtt ttttgtttct tgctattcac
     2701 cctgcaaatg tggtcttctt ttcaggagag attgtacatg cacggatctg gacatacagt
     2761 acacatttgc caatattcca tccagacaat taaactcatc ggacatgtac gtgccgatta
     2821 atgatttgag acaggatgtg gatcatgaaa ggataaatac tgttcacagg atacaggaaa
     2881 agcggtatga aagcaatacg ttcaagttta acggaagcct gagtagaaac ggttttgaaa
     2941 agtattgcag gatggatttg gaggcacagg agtttataaa agaggcacaa aaaatttata
     3001 gatgcagcat ggtagacatg gacaaggtgt tcagggtgtc aagaacaata gcggaccttg
     3061 atttacgaga gacgatatcc aagtttgatg tggctgaagc tttgggttat gtatcgtgtt
     3121 atatttattt aaaaactact actatataat acgaggagga ttgtttaatg tattctgata
     3181 atcttgtaca tatttggatt actttgcttg gaagacctgc aaacaaaatt gcgagtctgc
     3241 tggtagaaaa ctatgggagc ttgagagaag cttatttggt taaaagttat actgatgtga
     3301 cttgtgaaat caggaataat cgtttattat gtcagagact aatggatgaa tccattaagg
     3361 acagggcagc aaggatttac gaaagggttt tggaaaaggg tgtcaaaata acgcatgttt
     3421 tcagggagga ttatcctgag gatttgaaaa atatatttga cccaccattc gttttgtact
     3481 attatggtaa attgccggat aaaaacacga ctctcttgtc ggttgtgggt tcaagaaagt
     3541 gttccgcata tggaagaaaa gtcacatatg acatgtgttt caatattgca aaacacggtg
     3601 ttggaattgt aagcgggatg gcaaaaggca tagatgccag agctcatgtg ggatgtcttg
     3661 atgcaaatgg atacacaatt gcggtactgg gaggaggacc tgacaacatt tatcctttgg
     3721 agaacgaggt tatatactac aggataaaag agaacggact tgttctttcg gagtatccgc
     3781 cgggcacaat gccgcttaag catcattttc cagcaagaaa cagaatcatc gccggtcttg
     3841 gcaaagggct tcttgtgtgc gaggcaggaa agaaaagcgg cgccatgata actgtgaact
     3901 ttgcaataaa tgagggaaga gatatatttt ccgtacctgg caatattgat tcgagttaca
     3961 gcgaaggctg caacagcctg ataaaggctg gagcctgtat cgccaccagc tttatcgatg
     4021 tgttggaagg tttgaatttg attgaaggca ggaaaattgc cgggactgtc ggctggctga
     4081 atggtttgga caaggaagag gcaatggttg cagacttgat ttacaaaggt gttaaaatcg
     4141 aggatatttg cagcaaaacc gggttggaca gcgcaagagt cgttgcgatt acgacgattt
     4201 tggaagtgaa gggtatattg ggaagggata gacatggtaa tctgcacgtt gtaatataat
     4261 attgatggaa aatgcggatt gtgctatcat gagttggttt taaattaagc aaacatattt
     4321 ggaggaaagt aaaaatggct gattatcttg ttattgtgga gtcgcccaca aaagcaaata
     4381 caataaagaa gtttttaggc agaagctaca aggtcgaagc gtcggccggg cacgtaaggg
     4441 atttgccaaa aagcaaattg gcaattgata tagagaatga ttttgaacct caatatatta
     4501 acataagagg aaagggtgac ttgattaaaa cccttaagaa ggaagccgga aaggcaaaaa
     4561 aggtatatct tgcaacggac ccggacaggg aaggagaggc catttcgtgg catctgacac
     4621 agttgctgga tattgatgcc aatgagcctt gcagggtcac attcaatgaa atcacaaaaa
     4681 atgctgtttt ggaaggtttt aagaatccaa gaccgataga tatgaagctg gtggacactt
     4741 atcaggcacg acgtgttttg gacaggattg ttggctacaa gataagtcct gttctgtgga
     4801 aaaaggttaa aaaaggactt agtgcgggaa gggtgcagtc tgttgccaca agacttatat
     4861 gcgacaggga agacgagata gaagccttcg agccaaagga atactggtcc attaccgctc
     4921 tgttgtcaaa gtttggcaaa aaggataggt ttgaggccaa attccacgga aaacaaggtg
     4981 gaaaaataga ggttgaaaac gaggaacagg caaaaacaat ctacgatgaa atagaaaaca
     5041 gtgagttcaa ggttgttgat ttaaagaaat ctgaaagaag aagaagtcca gcgccacctt
     5101 ttacaaccag cactctacag caggaggcat ccagaaggtt tgggttccct gcttcaaaga
     5161 cgatgatggt tgcgcagatg ctttatgaag gaattgattt gggtggaaaa cagggtgtta
     5221 ccggtcttgt tacgtatatt agaacggact ccgtaaggat ttcggacaat gctcaaaacc
     5281 aggctcttga atatattaaa ggcaattttg gagacgagta tgttccaaaa aagaaaagag
     5341 tttacaaaaa caagaatcag gcccaggatg ctcacgaggc aataagacca acttatcttg
     5401 agcatgcccc tggcaagata aaggacaaac tgacaaacga tcagtacaaa ttgtacaaac
     5461 tgatatacga gagattcatc gcttcccaga tggctgaagc agtttatgat acggttagtg
     5521 tagacatact cacatcaaaa ggagattatc ttttcaaggc ttcaggtagt acattgaagt
     5581 tcaagggata caccgcaata tatatggatt ccaagagcga taacaatgac gaaaatgaag
     5641 aggatggcaa caggaagctg cctaaactgg aggttggcga ggttctaaat cttgacaagc
     5701 tggataaaaa gcagcacttc acacagcctc caccaaggta tacggaagca tcccttgtca
     5761 aggctctgga agagcacggc attggaagac ctagtactta tgctcctaca atttcgacaa
     5821 ttctggcaag aggatatgtc agaagggaaa agaaggtcct gtttccaacg gaactcggaa
     5881 ggatagttaa tgaccttttg aaaaagagtt ttaatgacat tgtaaacgtg gagtttacgg
     5941 caaatattga agacaggttt gacaagattg ccgagggaga ggaaaactgg gtttctgtca
     6001 tcagggattt ttacactcct tttgaaaaac aggtacaaaa ggctgaggaa gaactggaaa
     6061 aagtaa
//
LOCUS       DAONVY010000071         6005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9843_length_6005_cov_4.743193, whole genome shotgun sequence.
ACCESSION   DAONVY010000071 DAONVY010000000
VERSION     DAONVY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9843_length_6005_cov_4.743193"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..548)
                     /locus_tag="PLI11_03045"
     CDS             complement(240..548)
                     /locus_tag="PLI11_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51872.1"
                     /translation="MGCLLMPFELLFDLIIEGWFSLMKGILPKKTYSTGFYVVLKIII
                     GIYSMILLVIFIIGILSAILTEATVFDLWKLIFIPLGLSIAQIVLGIVVHYITKNNAQ
                     "
     gene            complement(763..1869)
                     /locus_tag="PLI11_03050"
     CDS             complement(763..1869)
                     /locus_tag="PLI11_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51873.1"
                     /translation="MDKVYKRINWVTTTCNGSMDPNRYLSNQVARAICEAAYEKPLTV
                     EEISLKTGLPTMYIEDALPNLIYGDAIEQVGKKYVTDFIILRHKDKSQMEKGFAPIVS
                     DVANYFEKLFNDYSRNIAAMSFYGHDFGMNKLGYIALPLALREKIRNIKNSLPNLENG
                     AYPPRKDGGYGWFIIQEAEDENDNAGEYMSGCNATDEEDGVIYCYNIIKYFNNDIYHN
                     GGTRWLSQNQIPQKCKNGIIPDDLLSEDDIVRLLQLNLIIKDDNCKNRYSLNFACFTK
                     EQLLEFSNLFHIEDEKLDKLLIDLILSIKKSFVNFVPKRLESQINQWVSCYTHTIIGY
                     VTDELISRGILEKPDDEKPLTNGVFFIEGKYINV"
     gene            complement(2095..2358)
                     /locus_tag="PLI11_03055"
     CDS             complement(2095..2358)
                     /locus_tag="PLI11_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HPZ51874.1"
                     /translation="MKASENEIVRFIEPVFYFCLKRVNNRADAEDLASEIMLHVLDGV
                     RKYDIKSLEGWVWRIAHNRYARFYESWKNKSNASVDEFAIDLV"
     gene            complement(2844..2926)
                     /locus_tag="PLI11_03060"
     tRNA            complement(2844..2926)
                     /locus_tag="PLI11_03060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2890..2892),aa:Leu,seq:tag)
     gene            complement(2977..3243)
                     /locus_tag="PLI11_03065"
     CDS             complement(2977..3243)
                     /locus_tag="PLI11_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51875.1"
                     /translation="MFRLIYNKKLLVDSSAEEFARVRANLDEKNIPCYIKTVKSSPTI
                     LQSSYAQSYQRYAMSYSPAKDHVSYVYYIYVKRKDYDKARLAME"
     gene            3358..3864
                     /locus_tag="PLI11_03070"
     CDS             3358..3864
                     /locus_tag="PLI11_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPZ51876.1"
                     /translation="MNDKNMEEIFTKHAQTVYKYLYSMTRNEHLSEELTQETFAIAVS
                     SINRFRGECKISVWLCQIAKYQWYKELERRKKYKMESIDIYDNSLFTAGFENEIEFKD
                     VVKILLEELESLDPATQEVIRLRIFGNMSYKEIGEVLGKTENWARVTFYRGKLKLKER
                     MEKDEGQS"
     gene            3848..4657
                     /locus_tag="PLI11_03075"
     CDS             3848..4657
                     /locus_tag="PLI11_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPZ51877.1"
                     /translation="MKDNLRCEIVEDLLPLYTDNLTSYFTSEAVKNHLSSCERCSKVY
                     EIMIEPVENEKIEKKEIDWFRKIRRNKYTLALIAVFLMSTFVILYDFFTSFTSVRIDV
                     MWTYLYYTKVEKWLIPYYAISILSLFLLIIIWYKNNRHRIGKKAKQAACIILLIILVL
                     NFAYQTTSYFHYEGLKKELKIIAEGKSDIRPSMTFVQCLKEEYISSSEYWEHDISTPL
                     ILHAFGKGKVYLVYSITSEHTGTSNAPVIISYKLMKGKVVITDIDESGHGV"
     gene            4742..5302
                     /locus_tag="PLI11_03080"
     CDS             4742..5302
                     /locus_tag="PLI11_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPZ51878.1"
                     /translation="MGIGEIILTAFALAMDAFSVAICNGLSAKKSTLKQAAATAIFFG
                     GFQALMPLLGFYIGKQFEDIITPIDHWIIFFLLAFIGVKMIMESSSCEVESNAFALKN
                     LFSLAVATSLDALAVGISFAWLKVNIIVAVLFIGVTTLVLSFLGVKIGTYLGSKFKTC
                     SERIGGVVLILIGLKILLEHLGVLPF"
     gene            complement(5299..>6005)
                     /locus_tag="PLI11_03085"
     CDS             complement(5299..>6005)
                     /locus_tag="PLI11_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HPZ51879.1"
                     /translation="YEDRPEDITLMRIGTYHLVEMTQKDGKWSIDREWYTDPFADSLE
                     LDNLKMEQNKKFILSNEARDFSNLNPRRLGVIEYADRYCGAAASGEHGYSYNPKYKNY
                     NSIGGDCANFASQCMHEGGKLRKNSVWNYDKEGSMAWLSARGLYSYIVSSGRGSLIVS
                     GNYNTVLKLSYKLLPGDIVAYEKNGKIAHISVVTGQDSRGYALVNCHNTDRYRVPWDL
                     GWSNKKIKFHFIRVHY"
BASE COUNT         1847 a         1187 c         1084 g         1887 t
ORIGIN      
        1 ttctcgcaaa cattcattca tctcacaagg tgtattctat ttgacgctta cgttttagca
       61 cttccaaagc atcggtattc gttcgtacca gttctgcaat ctatgccaaa cgcacgtcca
      121 tgaattgatt aaattcttgg ttgttgctct cttttaagct tacaactgac ttcttgtaag
      181 atgtttctgt agagaggtaa caaaaaggat gcaggtgatt ttaaggcact atgctctttt
      241 tattgggcat tattttttgt aatgtaatgg acaactatcc ctaaaacaat ttgagcgata
      301 ctaagtccaa gaggaataaa aatcagtttc catagatcaa aaactgttgc ttcagttaat
      361 atagcgctaa gaatacctat gataaagatc accaatagaa tcatagagta aatcccaatt
      421 ataattttta atacaacata aaacccagtg ctataagttt ttttaggtaa tattcctttc
      481 attaatgaaa accaaccttc aataattaaa tcaaataata gttcgaaagg cattaacaag
      541 caacccatag aatactccct taaatgtaaa cataaaaaac ctgcggtgca cgaaatactc
      601 acctcgtaaa gatgatatac tcaaccatct tggttcactg tacgatagac atgcactgct
      661 gtaagaaccc ttctatcctc attattgata atatactttc gaaaccagtt caattacaaa
      721 gagtgcatgc cgaaagcaga ttgatctact ttcggtatat cattacacat ttatatactt
      781 tccttctata aagaacacgc cgtttgtgag tggtttctca tcatcgggtt tttcaagaat
      841 acctcttgat atcagctcat ctgttacata accgataatt gtatgtgtgt aacaggaaac
      901 ccattgatta atctgacttt caagtcgttt tggcacgaaa ttaacaaagc tctttttgat
      961 gctgagtatc aaatcaataa gcaacttatc cagcttttca tcttctatat ggaacaaatt
     1021 tgaaaactct aaaagctgct ctttggtaaa gcaagcaaaa tttaagctat acctattctt
     1081 gcaattatca tctttaatga tgagatttag ttgcaacagc ctgacaatat catcctcaga
     1141 gagcaagtcg tccggaatga tcccgttttt acatttctgg ggtatctgat tttgagaaag
     1201 ccatcttgta ccaccattat gatagatatc gttattgaaa tacttgataa tattataaca
     1261 atagataaca ccatcttctt catctgttgc attgcacccg gacatgtact caccggcatt
     1321 atcattttca tcctcagctt cctgtatgat aaaccacccg taaccgccat cctttcgtgg
     1381 cggatatgct ccgttttcaa gattcggcag actgtttttt atatttctta tcttttcacg
     1441 tagcgcaaga ggtagagcga tatatccaag tttattcatt ccaaaatcat ggccataaaa
     1501 gctcattgcg gcgatatttc gcgagtaatc attaaaaagc ttttcaaagt agtttgcaac
     1561 atcactgaca ataggtgcaa aacccttttc catttgtgac ttatccttgt gccgaaggat
     1621 tataaaatca gtaacatatt tctttcccac ctgctctatg gcatcaccgt aaatcaaatt
     1681 aggcagtgca tcttcgatat acattgtggg tagacccgtt ttaagactga tttcttcaac
     1741 tgtcagcggc ttttcataag cggcttcaca aattgcacgg gcaacctgat ttgaaagata
     1801 cctgttgggg tccattgaac cattgcaagt tgtagttacc caattgatac gcttgtaaac
     1861 cttatccatt ttatcctctc ctatcctgtc tcgtaccttc tgacgactta cattgagtcg
     1921 ccatttgatt gtggtttctg gtaggttata cttttcggca agtattttta ttggcatttc
     1981 tccgatatag taatctacca gaatgttctt gtattcggag gatagcgtgt gaagataacg
     2041 gaaaacctgt tcatattcat ctgcgattgt cgtatcatct acgacatcat aatctcacac
     2101 gagatcaata gcaaactcat caacagaagc atttgatttg tttttccatg attcataaaa
     2161 acgagcatac cggttatgag caatacgcca tacccagcct tcaagtgact taatatcata
     2221 ctttcgcaca ccatcaagta catgaagcat tatttcactt gcgagatctt cagcatctgc
     2281 acgattgttg acacgtttta aacaaaagta aaaaacaggc tcaataaatc gtacaatttc
     2341 gttttcgcta gctttcacgc tgcctcctcc tttcgttttg gttcataatc atgattacac
     2401 ccatatagtg ccggaaaatt tagatggata ggtactttgg aatatatttt atcaaaaatt
     2461 tctgtccgat tctacaacaa tgcaatgctg cacctgaatt gtcagtatca cagtgagcga
     2521 ttttatcata cattgagaca tttggctgac acttgttgtg ttaaaatttc agatagatct
     2581 tgaaaaagtc ataattttac tctctactca aaaacggagt tgaaatcgag gtggagagtt
     2641 agtgctccac cttatttttt tgtcttgtga accctttcgt tagcgcgggt atagaaaaaa
     2701 gagctgcttt acaataaatt gtgtaatttt gatcctgaac cggcagtaat gagaaaggat
     2761 aaaagtgacg aaaatgtact aattacaagc atttccgaca taaaaaaaga acgcaggctt
     2821 tgtttaatcc taacgttctt atctggtacg ggtaacagga gttgaacctg cacggttgcc
     2881 caccggaacc taaatccggc gcgtctgcca gttccgccat acccgcgtag acattatcat
     2941 atcagaaaag tgtctatttt gcaagtactt ttagtgctat tccatagcca gtctagcctt
     3001 gtcataatcc ttgcgtttga cataaatata atagacgtaa gagacatggt cctttgccgg
     3061 agaataagac atggcatatc tctgatacga ttgggcatag gaggattgca gtattgttgg
     3121 ggaacttttt acagtcttta tataacaagg tatgttcttc tcgtctagat tcgctctaac
     3181 cctagcaaat tcttctgcag aactgtcaac caaaagcttc ttgttgtaaa tcagcctgaa
     3241 cattattcca acccctttca gtcaaattat accacaaatg taataagtaa agaataatta
     3301 attgtaattc tgaaacaaaa gtcctagaat cagtacatac atattggagg tgacactgtg
     3361 aatgacaaaa acatggaaga aatctttaca aaacatgccc agaccgtata taaatatctg
     3421 tactccatga ctagaaatga acacctttca gaggaactga ctcaggagac ctttgccatt
     3481 gcagtctcct ccataaaccg ctttagaggc gagtgcaaga tatcggtatg gctgtgtcag
     3541 atagcaaagt atcagtggta caaggaattg gaaagaagga agaagtataa gatggaatcc
     3601 attgatattt atgacaacag tctttttaca gcaggctttg aaaatgaaat agagtttaaa
     3661 gatgtagtaa agatactgct tgaggagctt gagagccttg accctgcaac acaggaggta
     3721 atcaggctta gaatatttgg aaatatgagt tacaaagaga ttggagaagt gctgggaaaa
     3781 acggaaaact gggccagagt caccttctac agaggaaaac tgaaactaaa ggaaaggatg
     3841 gaaaaggatg aaggacaatc ttaggtgtga aatagttgag gatttgctgc ccctttatac
     3901 agacaatttg acaagttatt ttacaagtga agctgtaaaa aatcatttgt cttcatgtga
     3961 aagatgctcc aaagtttatg aaatcatgat agaacctgtt gagaatgaaa agatagaaaa
     4021 gaaggaaata gactggttta gaaaaatcag aaggaacaag tacactcttg cacttattgc
     4081 agtcttttta atgagcacct ttgttatcct gtatgatttc ttcacctcat ttacatcagt
     4141 cagaatagat gtaatgtgga cgtatctcta ctatacaaag gttgagaaat ggctgattcc
     4201 atattacgca atatcaattt tgtccctttt tttacttata attatttggt ataaaaacaa
     4261 cagacatcgg attggaaaaa aagcaaaaca agctgcatgc attatactgc ttataattct
     4321 agtgttaaac tttgcctacc aaacaacctc ttactttcac tacgaaggac ttaaaaagga
     4381 gcttaagata attgcggaag gtaaatccga tatacggcca agcatgacct ttgtgcagtg
     4441 tctaaaggaa gaatacatct cctcctctga gtattgggag cacgatattt caacccctct
     4501 tattcttcat gcctttggca aaggaaaagt gtatcttgtt tattcaataa cgagcgagca
     4561 caccggaaca tcaaacgcac ctgtaataat ttcatacaaa ttgatgaaag gaaaagtcgt
     4621 aattacggat attgacgaat ccggtcatgg cgtttgattt gcatatcaaa ataaaaaggt
     4681 atataatcta tccgttcgta taaaaatgtt atacgcactt gtatatttga ggagttgtaa
     4741 gatgggtatt ggagaaatca tactgacagc ctttgcactg gctatggacg ctttttctgt
     4801 cgccatatgc aacgggcttt cagcaaaaaa gagtacatta aaacaggctg ctgcgacagc
     4861 catatttttt ggtggattcc aggcattgat gccacttttg ggtttttaca taggaaaaca
     4921 atttgaagat ataattacac cgatagatca ttggataata tttttcctgc ttgcattcat
     4981 aggagtcaaa atgatcatgg aatcaagctc ttgcgaagta gaaagcaatg cttttgcatt
     5041 aaagaatctg ttttctcttg cagttgccac cagccttgat gctctggcag ttggcatttc
     5101 atttgcatgg cttaaagtaa atattatcgt tgctgttctt tttataggag taactactct
     5161 tgtactttca ttccttggtg ttaagattgg cacctatttg ggctcaaagt tcaaaacctg
     5221 ttcggaaaga attggaggag ttgtccttat ccttattgga cttaaaatac tcttggaaca
     5281 cctgggggtt ttgccttttt aatagtggac ccttataaaa tgaaacttta ttttcttgtt
     5341 actccatccc aaatcccaag gcacccgata tctgtccgta ttatggcagt ttacaagagc
     5401 ataacctctc gaatcctgcc cagtcacgac ggatatgtgc gctatttttc cgtttttttc
     5461 ataggcaact atatctccag gaagcagctt gtatgaaagc tttaatactg tgttgtaatt
     5521 tccacttaca atcaacgagc ctctcccgct tgaaacaata tagctgtaaa gccctcttgc
     5581 gctcagccag gccatgcttc cttccttgtc gtagttccag accgaattct ttctcagttt
     5641 accaccttca tgcatgcact gggaagcaaa atttgcacag tctcctccaa tggagttata
     5701 atttttgtac tttgggttat aggaatatcc atgctcgcca ctggctgcag cgccacagta
     5761 cctgtctgca tattcaataa ctcccagtct tctgggattt aaattactga aatctcttgc
     5821 ttcattcgat aatatgaatt tcttgttctg ctccatttta aggttgtcaa gctccaggga
     5881 atccgcaaaa ggatctgtat accactccct gtctatggac catttcccat ccttttgtgt
     5941 catttcaaca agatggtaag taccaatacg cattagagtg atatcttccg gtctatcctc
     6001 atacg
//
LOCUS       DAONVY010000072         5982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9887_length_5982_cov_4.395816, whole genome shotgun sequence.
ACCESSION   DAONVY010000072 DAONVY010000000
VERSION     DAONVY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9887_length_5982_cov_4.395816"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLI11_03090"
     CDS             <1..298
                     /locus_tag="PLI11_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="seems to be involved in biofilm formation; in
                     asymptomatic bacteriuria E. coli strains 83972 and VR50,
                     the ygiD gene is upregulated during biofilm formation in
                     urine; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HPZ51880.1"
                     /translation="SGNIVHNLRMVDWNMENKGYDWAYEFDDYIHDNIMDANHGRIML
                     YHQAGECAKYSVPIPDHYYPLLYALGASSRNDEVSVYNKACVLGSVTMTSYLWE"
     gene            324..875
                     /locus_tag="PLI11_03095"
     CDS             324..875
                     /locus_tag="PLI11_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ51881.1"
                     /translation="MDVEIDLSNTIIETQRLVLRPWLESDLDDLYEYASVEGVGEMAG
                     WPHHKSIEESRNILHLFIADKNVLAVVFKENNKVIGSLGLHYSWANEEHEYKNLKIKE
                     IGYVLSKDYWGMGLMPEAVSAVIKFCFDKLNLNALTVGHFVENMQSRRVIEKCGFKIY
                     KKSEYYSEQLKKSFQDIKYILIR"
     gene            900..1415
                     /locus_tag="PLI11_03100"
     CDS             900..1415
                     /locus_tag="PLI11_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPZ51882.1"
                     /translation="MKILKEIMRKPNVNTVGKTIARNAVRGVVLRGKTILMVYSTNGG
                     DYKFPGGGVDEGESFEEALVREIKEECGTEVLSINEEIGKVIEYDIPIEEDYDIFKRI
                     SYYYSCDVDLNFGKQSLDDYEIALGLIPVWVDIDKAIEANKKLMRSYPCQDWVPRETF
                     MLEYVKERYNL"
     gene            1647..2555
                     /locus_tag="PLI11_03105"
     CDS             1647..2555
                     /locus_tag="PLI11_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPZ51883.1"
                     /translation="MEYITVKEAAAKWGVSERRVCAFCAEGRVEGVIRKGRSYLIPKD
                     AIRPIDNRSYRGVYIPKRYKTLFASIDKMKHETVSRNRLTRREIVKLLEDFLVEFTYN
                     SVAMEGNSLTLEEISQVLQGVTLEGKPLKDQLETVGYGDAARYVMSLLSDNIPISEWV
                     IREIHSLVMVDNYQHRGEYRSVNIASEYNHPKPDLVPVMMERLVASLDKGSRHPIEKA
                     VLFYLEYSRIHPFIEGNGRTGFLLLNLMLMQAGYPPIIIKYADKEKHIDGLNQYFNNT
                     DESPMVFLIGEYVRDILCQYLEILRE"
     gene            2984..3619
                     /locus_tag="PLI11_03110"
                     /pseudo
     CDS             2984..3619
                     /locus_tag="PLI11_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            complement(3641..5182)
                     /locus_tag="PLI11_03115"
     CDS             complement(3641..5182)
                     /locus_tag="PLI11_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51884.1"
                     /translation="MKRFFSFVLILILLTSTVPINADNEPTHKEEVVYGILNLDGSVK
                     GIHVVNIFEGGKILDYGNYSEIRNLTSSEKIIKDGDRITIDTSSDKFYYQGTLKKKEL
                     PWVISIKYYFDNKEIKGDELGGKSGDLKIHITVKKNKNVNDVFFNNHALQISLSLDNR
                     LCRNIKSENATVAEAGSKKQLAYTVLPGFEADIVVTATVKDFEMDPIQINGIRLNMGM
                     DVDTGEFTRQISELVNAIRLLDEGADGLLDGAANLANGMQSYIDGLNAFKNGLSLLPD
                     SVNEIYNGVSQVSYGLNELAKQNDSLLAGALAIQQATFETVNMQLAGMGLPTLTPENY
                     SAILAPIPQLAEVKLKLDEIVQFTEGLKSYLNGVGEISSGTKELERGMSEFKSSANTI
                     AASANEIYSAAVTINNGIKGLKEGLNEYKQGTGQLREGTKDLVAMIDTQLSEILSLIS
                     EGDDEVVSFVSDKNTNVDSVQFVLKTDSIEKPVSEKPDSPKSPTLSFWQRLLKLFGLY
                     KDKDE"
     gene            complement(5184..>5982)
                     /locus_tag="PLI11_03120"
     CDS             complement(5184..>5982)
                     /locus_tag="PLI11_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPZ51885.1"
                     /translation="ELDKMPYVTNVVSYSTYVGAEIPQEFVPKDVSKQFYSDNYVRIL
                     IYTDMKDEGKETFKTVETIKDTAGKYYDEHYLAGQSATLYDMKNVLDVDTNVVNGIAV
                     LGIFITLLVTFRSLSLPFILLFTIETAIWINLSIPYFMGSSLSYVGYLIVSTVQLGAT
                     VDYAILFTNSYLENRKSLSKKEAMTKTIQTNVSAILTSAGILASAGFAMALTTSIPVV
                     YDLGVLVGRGTLLSLAMVALVLPALLVLFDKAIMKTTLKNQFKMEGD"
BASE COUNT         1792 a         1018 c         1331 g         1841 t
ORIGIN      
        1 cagtggcaat attgttcaca atttgaggat ggtagactgg aatatggaga acaaaggcta
       61 cgactgggca tatgagtttg acgactacat ccacgacaat atcatggatg caaaccatgg
      121 caggattatg ctgtatcatc aagctggaga gtgtgccaag tattctgtgc caattcctga
      181 ccactattac ccgcttcttt atgcacttgg tgcatccagc aggaatgatg aagttagtgt
      241 atacaacaag gcatgcgtgc ttggttcggt aaccatgacc agttatcttt gggaatagac
      301 tttgattaaa gggagtgttt ttggtggatg tggaaattga tttatccaat acaataattg
      361 aaacacaaag gttggtgctt cgtccctggt tggaaagtga ccttgatgat ttgtatgagt
      421 atgcatcggt agaaggcgtt ggagagatgg caggctggcc gcatcataaa tctattgaag
      481 aatcaagaaa tatcttgcat ttattcatag cagataaaaa tgtgcttgcc gttgtattca
      541 aggaaaacaa caaggttatt ggttctttgg gcctgcatta ctcatgggcc aatgaagagc
      601 atgaatacaa gaaccttaag ataaaagaaa ttggttatgt tctttctaaa gattactggg
      661 gcatgggcct tatgcctgag gctgtcagtg cagttattaa gttttgcttt gacaaactga
      721 atttgaatgc tttgacagtt ggacattttg tagaaaatat gcagtcaaga agggttattg
      781 agaaatgcgg tttcaagatt tataagaaaa gtgaatatta ttcggaacaa ctaaagaaga
      841 gttttcagga cataaagtac atattgattc gataaagaag agtttggtat aggtgagcta
      901 tgaagatatt aaaggaaata atgagaaagc cgaacgtcaa cacagtaggc aaaacgattg
      961 caaggaacgc tgtaagagga gttgttttaa gaggaaaaac aatcctaatg gtgtactcga
     1021 caaatggtgg tgattacaaa tttccaggcg gaggtgtcga tgaaggagaa tcctttgaag
     1081 aggcccttgt cagagaaatt aaagaagaat gtggtactga agtactgtct ataaatgagg
     1141 agataggcaa ggttattgaa tatgatattc ctattgaaga ggactatgat atctttaaga
     1201 gaatatcata ttactattca tgtgatgtag acttaaactt cggcaagcag tcattggatg
     1261 attatgagat agctcttggt ctaattcctg tatgggttga tattgataaa gctattgaag
     1321 ctaacaaaaa gcttatgagg tcctaccctt gtcaggactg ggttccaaga gaaacattta
     1381 tgcttgaata tgttaaagag aggtataacc tttaatgagt gacaagttta tcaaaactgc
     1441 ggtaaacgca gacctgggtg acaaaagtcc tgaaaggtgg tgacatgtac gagcaactgg
     1501 gtaaagtcca tgtcctgcga aattctgcat gttgacggaa caaggctcgt gcatgagata
     1561 attgatataa tagccaattt attgggatgg tgtaaaacaa agggaaaagc agatacttga
     1621 aaggtataaa aatgggagga tgggttgtgg agtatattac agtaaaggaa gctgctgcga
     1681 aatggggtgt ttcagaaagg cgcgtatgtg ccttttgtgc ggaaggtaga gtggaaggag
     1741 taattagaaa aggcaggtcc tatctaattc ctaaagatgc aattaggcct atagataaca
     1801 gaagctacag gggagtatat ataccaaaaa ggtataaaac actgtttgca tcgattgaca
     1861 agatgaaaca tgagactgtc agcaggaaca ggctgacaag aagagagata gtaaaactgt
     1921 tggaggattt cctggtggag tttacataca actccgttgc catggaaggg aacagtttga
     1981 cgcttgaaga aatctcacaa gttttacagg gagttacgct tgaaggcaaa cccttgaaag
     2041 accagcttga aactgttgga tatggagatg cagcaagata tgtcatgtct cttttaagcg
     2101 acaatattcc tatttccgaa tgggtgattc gtgagattca ctcccttgtc atggttgata
     2161 attatcagca cagaggtgaa tacagaagcg taaacattgc gagcgaatac aatcatccaa
     2221 agcctgatct ggtccctgtt atgatggaaa ggttggttgc ttcacttgat aaaggcagca
     2281 gacatccgat tgaaaaagca gttctttttt acttggaata cagcaggata catccgttta
     2341 ttgaaggtaa tggcaggaca ggattcttgt tacttaatct aatgcttatg caggcaggtt
     2401 acccgcccat tattatcaaa tatgcggaca aggagaaaca tatcgacggt ttgaaccagt
     2461 attttaacaa cacagatgaa tctccaatgg tttttctcat aggtgaatat gtaagagata
     2521 tattgtgcca gtacctcgaa attttaagag aataacaaaa aagtacaaaa tatctgttat
     2581 tctttaaaaa ctcgtgaaaa ccgtatgata gaattaggtt tgcatgagtt tttgaggtga
     2641 taacttgaac aaatattaca taaacaattg tgagattgac tcgatagatt tgaaatgcct
     2701 tgttgtcagt aaaggagtca aggtatcaaa agaggtatac aagaagtttt ccaaaacaca
     2761 taggttgggt ataaatcctc ttatgtgcaa ctcaatgttt ttatctgatg gcacaattgt
     2821 acagcttact gatatgagtt ttcatcttca gtatttaaca ggaatactgt cctgggacaa
     2881 tttaaaactg cttaaatatg ttcaggattt ggaaactcct ttttctttga aagtgtacga
     2941 aggtaaaccc accttgtttt actactccga atttgtggat gaggtaacca agcctgagga
     3001 tatggagctg tttcctcctt ctgaattggg tttttcctat cttatgctgt ttgacgacta
     3061 caataaagtg gaccttactt tgattccctt gaattgtctt gaggagtatt tgaaaagaga
     3121 caagcttgta acggtgcttc ttgacaagga taacaggata aaggagaaga ttataccaac
     3181 ggatgaggag tatcatatta ggaaacctac catgaggcag tttgatgact gctgcaatga
     3241 gttctggcat gtgtccacat atgttgttaa aggacttatg cgaagggaaa tactttattc
     3301 aatagctcat cttggtatag tgaggcggga gcttttgagg atgatgtcct ggaaggtagg
     3361 tcttgaaacg cacttttctc ttagtgtcgg taagaactat aaatatttga aaaggtatgt
     3421 ttctgatggt ttgtggaaca gacttatgac aacatataga atggatacgt atgaaaactg
     3481 ctggaactct ttgtttacat gtctcgatct ttttcgtgag gtttcaaaag aagttgcagt
     3541 taaactttgt tttgagtatc cagataatga tttcaacttg acaaagtatg taatggatat
     3601 atataaagga tatgaataaa ggctcttggg ggaagagcct ttattcatcc ttatccttat
     3661 acagcccaaa aagtttcagc aatctttgcc aaaatgaaag ggtaggggat tttgggctgt
     3721 caggtttctc ggaaactggt ttttcaatag aatctgtctt aagcacaaac tgtacagaat
     3781 ctacattggt gtttttatcg gaaacaaagg atacgacttc atcatctcct tcggatataa
     3841 gtgagagtat ttctgaaagc tgtgtatcta tcatggcaac caagtctttt gtaccttctc
     3901 ttagttggcc tgtaccttgt ttgtattcat taagaccttc ttttagtcct tttatcccat
     3961 tgtttatagt tacagcggcg ctgtagattt catttgcaga agcggctatt gtattggcag
     4021 aggatttgaa ctctgacatg cctctttcaa gctccttggt tccgctgcta atctctccca
     4081 ctccgtttaa atagcttttt agaccttctg taaactgaac aatttcatca agtttaagct
     4141 ttacttctgc aagttgtggt attggtgcaa gtattgcgct atagttttca ggggtaagag
     4201 ttggcaatcc catacctgca agctgcatgt tgaccgtctc aaaggtagct tgttgaattg
     4261 ccaatgcccc tgcaagaaga gagtcgttct gttttgcaag ttcattcaac ccataactta
     4321 cttgagaaac tccattatat atctcattca cactgtccgg aaggagagat aaaccgtttt
     4381 tgaaagcatt gaggccgtca atatagcttt gcattccatt tgccagattt gcggctccgt
     4441 caagcaaacc atctgcaccc tcgtcaagca gccttattgc attaaccagt tctgatattt
     4501 gtcttgtaaa ctcgcctgtg tccacatcca tacccatgtt aaggcgtatg ccgtttatct
     4561 gaatcggatc catttcaaaa tccttgacgg tggctgtgac tacaatatca gcctcaaagc
     4621 ctggcaagac cgtgtatgca agctgttttt tgctgcctgc ttctgcaact gtagcatttt
     4681 cacttttgat atttctgcac agtctattat caagcgacaa acttatttgc agggcatggt
     4741 tgttgaagaa aacatcattt acatttttat tctttttgac tgtaatatgt attttcaaat
     4801 caccgctttt gccgccaagc tcatcgcctt ttatctcctt gttgtcaaaa tagtatttga
     4861 tggagattac ccaggggagt tcttttttct tcaaagttcc ttgatagtag aacttatccg
     4921 aggacgtgtc tatggttatc ctgtctccat ccttaattat tttttcagag gaggtaaggt
     4981 ttctaatctc tgaataattg ccatagtcca gtattttccc gccttcaaag atgttaacta
     5041 catggattcc ctttacactt ccatcaaggt tgagaatgcc gtaaaccact tcttctttgt
     5101 gtgttggttc attgtccgca tttattggga ctgtagaggt tagaagtatc agtatgagaa
     5161 caaaggaaaa gaatcttttc actttaatca ccctccattt taaactggtt tttcaatgtt
     5221 gtcttcataa ttgccttgtc aaacagaaca agcaaagcag gtaataccaa ggctaccatg
     5281 gcaagggaca gcaaggttcc cctgccaaca aggacgccaa ggtcataaac cacgggtata
     5341 cttgtggtta aggccatggc aaaacctgcg ctggcaagaa tgcctgctga ggtaagtatg
     5401 gcagatacgt ttgtttgtat ggttttggtc atggcttctt ttttgctgag agacttcctg
     5461 ttttcaagat aactgttggt aaacagtatg gcatagtcaa cggttgctcc taattgaact
     5521 gtgctgacaa tgaggtagcc tacgtagctt aaggaactac ccatgaaata tggtatggac
     5581 aaatttatcc atatggcagt ttcaatggtg aagaggagga taaatggaag tgacaaagag
     5641 cggaatgtaa cgaggagtgt tatgaatatt ccaagtactg caatgccgtt taccacattc
     5701 gtatcgacat caagaacatt cttcatatcg tataaggttg cgctttgtcc agccaggtaa
     5761 tgttcatcgt aatatttgcc cgcagtatct ttaatcgttt caacggtttt gaaggtttct
     5821 tttccttcgt ccttcatgtc ggtgtatatg agtattctta cataattgtc tgagtagaac
     5881 tgttttgaca catccttagg aacaaattcc tgaggaattt cagcgcctac ataagttgaa
     5941 taagaaacca cgtttgtaac ataaggcatc ttatcaagtt cg
//
LOCUS       DAONVY010000073         5970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9912_length_5970_cov_3.952325, whole genome shotgun sequence.
ACCESSION   DAONVY010000073 DAONVY010000000
VERSION     DAONVY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9912_length_5970_cov_3.952325"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PLI11_03125"
     CDS             complement(<1..958)
                     /locus_tag="PLI11_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51886.1"
                     /translation="MYIWTASANERVYKNTRITRTSMSFLVLESAKNAYVSGQILLRS
                     IEGFEILDVKAHTEFPCDIYYQRYNVFNDGLPYPDELVAIKHVGRLSVLPHSTQGIWL
                     NFFIPRDVKEGRQDFFVEIFTSQGNFTVPVYLHIYNVIIPDTKDSEFNLEYFFLPFDF
                     FPYKDKKEQKNFANYEYERYSDKWWALMDEYAKSFLQIRNNSLDIRIMPLLADAGSRE
                     VENNKWEFNWELFDRFIKFFIDRNAVKSLSCCSIINPVMNDTVLCLDENSNIVSVEIE
                     SEKGEAFTDAFYCALYEHIKELGLLDRFRMRLQDEPHQDKYWI"
     gene            complement(1037..3352)
                     /locus_tag="PLI11_03130"
     CDS             complement(1037..3352)
                     /locus_tag="PLI11_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPZ51887.1"
                     /translation="MKKISRVLCIIFTILLVLTVGCTKEDDKLNFTKEQLRRVKFDND
                     KVLDKHIFGHNQCSHDFTKDRKEGKVLKLSTSINQANDPFVSLNLGDYLREIGDDSVK
                     GDEYKVVLLKIKVSDMISCNFELFYATGNSTTIEIGHSKTSIYDNSSEKWQYVLFDMT
                     KDPNWTDSINVLRFDFATAAVSKGETMYISEIMFAKDMDIACQVMGLDISTELENILD
                     AKTEEYIKKLLSIEDPETEYSKYAPLTAANEDKNINMWFNHTYTRTPEGETTPGDMKT
                     YQLRLAKNELEACQLIFSTKQDYSNMTLEITDFTNKNGKTLRTELLYGYYFEVREEMI
                     IDPLPPLDGPFDLKANKSKTFVIKVMSTLDSEAGEYVATVTLKDSSGQEVKKAKVFAY
                     VWDFELPEETSCKTLMDTSSFAVYTLHGVHEGDDEQLYKNYYDFLLENRINSYTLPYS
                     TEYYSDPRIVEYLDNPRVRAFLNMGWKRKLTGDNVKHSYDFLSQKQEWLDKTYFYPVD
                     EPIDITMLDQVRTFGEIIKQNFPNHKLLVPMHFNLSMNPESTMDHFEYVADYVTAWCP
                     KTFFFNTYEDYKKNPYLTYYMSGMLEKNLGTFPERMAKHQAEGDDVWWYVTRVPNYPE
                     ITLTMETKAVKYRVLFWQQKLYNVDNFLYYMTNDWYPEGNDLAWNAKHEKTHLYDVYG
                     NGVLVYCGAYVGICGPVGSLRLECVRDGIEDFEYLTILEKHYGKETVDNVISRITTSL
                     YKYTEDETTFTEVKIALGNLIEKTIKEGVIK"
     gene            3515..4750
                     /gene="eno"
                     /locus_tag="PLI11_03135"
     CDS             3515..4750
                     /gene="eno"
                     /locus_tag="PLI11_03135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPZ51888.1"
                     /translation="MRRIKDVYGREILDSRGNPTVEAVVILNDGTVATGMVPSGASTG
                     EYEALELRDGGTRYGGKGVQKAVKNIDGPIRDAVVGLDVTDIYTIDRLMIEADGTKEK
                     KKLGANSILAVSLACSHAAAKSLNIPLYRFLGGAAGTKLPRPMMNILNGGAHADNNVD
                     IQEFMILPTEEDFTVSLEQCANVFHTLKALLKSKGLSTAVGDEGGFAPNLSDDTEAIE
                     LILQAVEKAGYKVGQDFNIALDAAASEWKTDKGYLFPKKNKEMTVEELVSYWEKLVSD
                     YPIVSIEDPLGENDWDAFTELTKRIGDKIMVVGDDLFVTNPERLVKGIELNAGNSILI
                     KLNQIGSVSETLKTIRIAQDAGFNVIVSHRSGETEDTTISDLAVATNAGFIKTGAPSR
                     GERVAKYNRLLRIADKRPC"
     gene            complement(4789..>5970)
                     /locus_tag="PLI11_03140"
     CDS             complement(4789..>5970)
                     /locus_tag="PLI11_03140"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPZ51889.1"
                     /translation="YIQKNGIDKYSLIILTGCEVISMETLDVLKEYYDKGGKIIATYT
                     LPTKTAEKGKDNEAKEIIEHIFGTDIYSTSAKGNIYKNTNKNGGQAYFICSYDYGTND
                     YFKNIDLLELAVNEAIKVRDVAINTNDDSYKSIVTYIHRKTEKEDIYFIANTGSDNFS
                     GSVVLKGKHHPVLLDPHTGEITVPEYKNHKLYGENVTSVKLDIETLSSKIIYCGDKYK
                     NIRMPLLYKGELETFNEAGIEFDNGTISFIFSIGQDDKLELAARLDENDNGYILEIED
                     GRINLLKKQDDKTDIINSVVVPIHRNNFYQFTMTVNEDGFDVFLSGRKLFEATDSTFK
                     QGRIAFGNIDDSSIEVFDIVLLGGLEKVEVVNHSYTVYLVLFLSLILLRLSAMLHKKK
                     K"
BASE COUNT         1693 a         1199 c         1142 g         1936 t
ORIGIN      
        1 atatccaata tttgtcctga tgcggttcat cttgaagtct cattctgaat ctgtcaagaa
       61 gaccaagttc cttgatatgc tcatacagag cacagtagaa tgcatctgta aaggcctccc
      121 ctttttcgct ctctatctct acagacacta tattggagtt ttcgtcaagg cacaaaaccg
      181 tgtcattcat tacaggatta attattgaac aacaggaaag gcttttcaca gcatttctat
      241 caataaagaa tttgatgaac ctgtcaaaca gttcccagtt aaactcccat ttgttattct
      301 caacttccct tgaacctgcg tctgcaagca gaggcatgat tctaatatcc aaagagttgt
      361 ttctgatttg caaaaagctc tttgcatatt cgtccataag cgcccaccac ttatctgaat
      421 acctttcata ttcgtaatta gcaaagttct tttgttcctt tttatcctta tacgggaaaa
      481 agtcaaaggg caggaaaaag tattcgaggt tgaattccga gtccttggta tcaggtataa
      541 tcacattgta aatatggagg tataccggta cggtgaaatt tccttgagat gtgaagatct
      601 caacaaagaa atcttgccgg ccttctttta catctcttgg tatgaaaaaa ttgagccata
      661 ttccttgagt gctgtgagga agcacgctta acctgcccac atgctttata gctaccagct
      721 catcaggata cggtaatcca tcgttgaata cgttgtatcg ctgatagtag atgtcgcaag
      781 gaaattccgt atgtgccttt acatccaata tctcaaagcc ttcaatactt ctaagcaaga
      841 tttgacctga gacatacgca tttttcgcac tttcaagaac aagaaaactc attgaagtcc
      901 ttgttatgcg ggtgtttttg taaactcttt cattggcaga tgctgtccag atatacatgt
      961 ttatccctcc taaaaaatat ttatttccaa caaaataaac atgcgcatcg ggtcattacc
     1021 caatgcgcat taaactttac ttaattacac cttctttaat agtcttttca ataaggttgc
     1081 cgagtgcaat tttcacttct gtaaatgtcg tctcatcctc agtgtacttg taaagggatg
     1141 tggtaatcct gcttattaca ttgtccacag tttccttgcc atagtgtttt tcaaggatgg
     1201 taagatactc gaagtcctct attccgtccc ttacacattc aagtctcaag gagcctacag
     1261 gaccacagat tcctacataa gcaccgcagt acacgagaac gccatttccg taaacatcgt
     1321 acagatgtgt cttttcgtgt tttgcattcc aagcaagatc gtttccttct ggataccagt
     1381 catttgtcat gtaatagagg aaattgtcca cattatacag tttctgctgc cagaacagta
     1441 ccctgtactt aactgctttg gtttccattg tgagagtgat ttcaggatag ttgggaacac
     1501 gtgttacata ccaccaaaca tcgtcaccct ctgcctggtg ttttgccatt ctctcaggga
     1561 atgtgccaag atttttctca agcattccgg acatgtagta tgtcaggtaa ggattctttt
     1621 tgtaatcctc atatgtgttg aagaaaaatg tttttggaca ccatgctgtt acataatcag
     1681 ccacatactc aaaatgatcc atggttgatt ccggattcat ggacaagttg aaatgcatag
     1741 gcacaagcag cttgtggtta gggaagttct gctttatgat ctcaccaaag gttcttacct
     1801 gatcgagcat ggtgatatca ataggttcgt ctaccggata gaagtatgtc ttgtcaagcc
     1861 attcctgctt ctgggagaga aaatcatagg aatgtttgac attatctccg gtaagttttc
     1921 ttttccaacc catattgagg aatgctctaa cacgtggatt atccaagtat tctacaattc
     1981 ttgggtcact atagtattcg gtggaataag gaagcgtata tgagttgatt ctgttttcaa
     2041 gcagaaaatc ataatagttt ttgtaaagct gttcgtcatc tccttcgtga accccatgca
     2101 acgtataaac tgcaaaggag ctcgtatcca ttagggtctt gcatgatgtt tcctcaggga
     2161 gttcaaaatc ccaaacatat gcaaacacct ttgctttttt gacctcttgt ccgcttgagt
     2221 ctttaagggt aactgtggca acgtattcgc ctgcttcgct atcaagtgta ctcattacct
     2281 ttattacaaa ggttttactc ttgtttgctt tcaggtcaaa gggtccgtca agaggtggaa
     2341 gtggatcgat tatcatttct tccctgacct cgaaataata gccgtacaag agttcggttc
     2401 tcagagtttt tccgttcttg tttgtgaagt cggttatctc aagagtcatg tttgaataat
     2461 cttgttttgt tgagaaaatc aactgacagg cctccagctc gttctttgca agcctaagct
     2521 ggtatgtttt catatctcct ggagttgtct cgccttccgg agttcttgta tatgtgtgat
     2581 taaaccacat atttatattc ttatcctcat tggctgctgt aagaggtgca tactttgaat
     2641 attctgtttc cggatcttcg atggataata gtttctttat gtattcttca gttttagcgt
     2701 ccagaatgtt ttcaagctcc gtactaatat caagtcccat aacctggcat gcaatatcca
     2761 tatcttttgc aaacattatt tctgatatgt acattgtttc gccttttgac actgccgcag
     2821 tagcaaagtc aaatcttaaa acatttatgc tgtcagtcca gttagggtcc ttggtcatgt
     2881 caaacaacac atactgccat ttttcgctac tgttgtcata tattgatgtt ttggaatgtc
     2941 ctatttcgat tgtagtgctg tttcctgtag catagaaaag ctcaaagttg catgaaatca
     3001 tatctgaaac ttttatcttc agaagtacaa ccttatattc atcgcctttt acgctatcat
     3061 cgccaatttc tctcaaatag tcacccagat tgagactgac gaaaggatca ttagcctgat
     3121 ttatggatgt ggatagtttt aagactttgc cttcttttct atccttggta aaatcatgcg
     3181 agcattggtt atgtccaaaa atgtgtttgt ccaaaacctt gtcgttatca aattttactc
     3241 ttcttagctg ctctttcgta aagttaagtt tgtcgtcttc tttagtgcat ccaaccgtta
     3301 aaaccaataa aatggtaaaa attatgcata aaaccctaga aatttttttc attttacgct
     3361 tctccttttt tttacttgac taactcttat agcaacccca gtaattatac acagaaatat
     3421 atgtaatttg caagatggtt tttacaatta tttgccttga taataaaata gtgctattat
     3481 atgctcaaat aacatatctc aaggaggtag ttctatgaga agaattaaag atgtttacgg
     3541 tagagaaata ctggattcca gaggaaaccc aactgttgaa gcagtagtta tactcaatga
     3601 tggtacagtc gctaccggaa tggtgccatc cggtgcttca actggagaat atgaagcatt
     3661 ggaacttagg gatggcggaa ccagatatgg cggaaaaggt gtacaaaagg ccgtaaagaa
     3721 tattgacggc cccattagag atgccgtagt tggtcttgac gttacagata tatacaccat
     3781 tgacaggtta atgatagagg cggatggaac aaaagaaaag aaaaaactag gtgcaaacag
     3841 catcctggct gtttcccttg cttgcagcca cgcagctgca aagagcttga acatccctct
     3901 atacagattc ctgggcggag ctgcaggtac gaaacttcca agacccatga tgaacattct
     3961 gaatggtggt gctcatgccg acaataatgt ggatatacag gaattcatga tacttccaac
     4021 agaagaggat tttacggtat ctcttgagca gtgtgcaaat gtgttccaca ctttgaaagc
     4081 gctacttaag tcaaagggat tgtccacagc tgttggagat gaaggaggat tcgcaccgaa
     4141 tttgagcgat gatactgaag ctatcgagct tatccttcag gcggttgaaa aggcaggcta
     4201 caaagttgga caggatttca acattgcact ggatgcagca gcgagtgaat ggaaaacaga
     4261 caaaggatat ttgttcccta agaaaaacaa ggaaatgacg gtggaagagc ttgtttcgta
     4321 ttgggagaag ctagtaagcg attatcccat agtgtcaatt gaggatccat tgggagaaaa
     4381 cgattgggat gcctttactg aacttacaaa acgcattggt gataaaatca tggttgtcgg
     4441 tgatgacttg ttcgttacaa atccggaaag attggtaaaa ggaattgaac tgaatgcagg
     4501 caactcaatt cttatcaagc tgaatcaaat tggttctgtt agcgagacat tgaagactat
     4561 ccggattgct caggatgctg gattcaatgt aatcgtatcg catcgttccg gtgaaactga
     4621 agatacaacc atctctgatt tggctgtagc aacaaacgca ggtttcataa agaccggtgc
     4681 cccaagccgc ggagagaggg ttgcaaagta caacagactc ttaagaattg cagacaaaag
     4741 accatgctga ttattaagga gcccaaattt gggctcctta acatatcttt acttcttttt
     4801 tttatgtaac attgcagaca atcttaacag tatcaaggaa agaaacaaaa caagatatac
     4861 tgtatatgaa tgattgacta cctcaacctt ttcaagtcca cccagaagta caatgtcaaa
     4921 cacttcgatg ctggaatcat caatattgcc aaaggctatc ctgccctgct tgaaggttga
     4981 gtcagttgct tcaaagagct ttctaccgga caggaacaca tcgaaaccat cctcattcac
     5041 tgtcatggta aactgataaa aattatttct atgtatggga actacaacac tgtttattat
     5101 gtcagtcttg tcgtcctgtt ttttaagaag atttatcctt ccgtcctcta tttccaatat
     5161 gtatccattg tcgttttcat ccaatcttgc tgccagctca agcttgtcat cctggcctat
     5221 tgaaaatatg aaggatatgg ttccattgtc aaattcgatt ccagcttcat taaaggtctc
     5281 aagttcacct ttgtacagca aaggcattct aatatttttg tatttgtctc cacagtagat
     5341 tatctttgaa ctcaatgttt caatatccag ttttacacta gtaacattct ccccgtatag
     5401 tttatgattc ttgtactcgg ggactgttat ctcaccggta tgaggatcca aaagaacagg
     5461 atggtgtttt cctttaagga ctacgcttcc tgaaaaattg tcgctgcctg tgtttgcaat
     5521 aaagtagatg tcttcctttt ccgttttcct atgaatgtat gtaacgatac ttttgtaaga
     5581 atcatcattc gtattaattg caacatccct gacctttatt gcctcattaa cagccaactc
     5641 caacaaatca atgtttttaa aatagtcatt agttccgtaa tcataggaac atatgaagta
     5701 cgcttgtcct ccatttttgt tggtattttt gtatatgttt ccctttgctg aagtggaata
     5761 tatgtcggta ccaaaaatat gttcgattat ttcctttgct tcattgtctt ttcccttttc
     5821 agcggttttt gttggaagtg tgtatgtagc aataattttg cctcccttgt cgtagtattc
     5881 tttcaataca tccaatgttt ccatactaat tacttcacat ccagtcagga taatgagtga
     5941 atacttgtct ataccgtttt tctggatata
//
LOCUS       DAONVY010000074         5946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_9955_length_5946_cov_2.985232, whole genome shotgun sequence.
ACCESSION   DAONVY010000074 DAONVY010000000
VERSION     DAONVY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9955_length_5946_cov_2.985232"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLI11_03145"
     CDS             <1..867
                     /locus_tag="PLI11_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51890.1"
                     /translation="RELRDDDYVLPPDFDNMTTSMESFDKQWMYHAVSQTIIANTILR
                     QLEFVDKDKIGLTGISWGSVIASIAIGYDDRFLFAIPVYGSGYLYESLAWIKDNFNYP
                     GTKEIWEPSHRLNSVKMPVLWLCWTDDSCFSINSNDKSFADTKNGILSMKQDMQHGHI
                     EGWIAPEIYRFADCIVKGTKPFATCIEKIGTERNVSFYMDIPEDADEVTAKAYYIKEK
                     LSYSRNGRYKLEWCDTIDQEWFTVDCSVEGDVIHVKLPEDAHSYYIELTTHVCGNPYI
                     TTSRFLTLDVKS"
     misc_binding    914..1175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1229..>1524
                     /locus_tag="PLI11_03150"
     CDS             1229..>1524
                     /locus_tag="PLI11_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPZ51891.1"
                     /translation="MKNIVIGGAWPYANGSLHIGHIAALLPGDVLARYYRLKGDRVFY
                     VSGSDCHGTPVTIRARQEGRTPEEISGFYHQEFVRVFDKLGFSYDLYTKTTDKA"
     assembly_gap    1525..1534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1535..2876
                     /gene="metG"
                     /locus_tag="PLI11_03155"
     CDS             <1535..2876
                     /gene="metG"
                     /locus_tag="PLI11_03155"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPZ51892.1"
                     /translation="AHKEFVTEFHKKLYESEFVYEKSAPEAFCKQCGKVLTDRLVVGL
                     CPNCKKETRSDQCDNCSAVLESHTVIDPVCSECGNSVVFRETKQLYLAISKLKKHLSD
                     YLLSKPHWRKNAYAFTKRYLDEGLRDRAITRSIDWGIDVPRLGFEDKKIYIWAENVLG
                     YLSASKQVADERGVDFKEVWGSNARHYYIHGKDNIPFHTIILPALLIAHGDNLRLPDD
                     IVSSEYLTLEGRKISTSQNWAVWLKDIVDRYNSDSLRYFFLANGPEKRDSDFSWHEFA
                     ERNNSDLVGVYGNFVNRTLAFIVKYNNGVVPCGALEKDIEKKINDAYSEAGSLLEDGQ
                     FKGALEVIFDLVRFGNKYYDTMEPWKTRHGDKEKCFNTLYNCVQIIANLAVLLEPILP
                     FSSQKAIDWLGLDRAWKPQFVKEGHQLTDISILFERIDKDVIEEERNRLKITNT"
     gene            3058..3789
                     /locus_tag="PLI11_03160"
     CDS             3058..3789
                     /locus_tag="PLI11_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPZ51893.1"
                     /translation="MRLNRYISSTGLCSRRQADELIKQGKVKVNNTVAVIGTDVSPDD
                     VVEVNGKLLKGKSEYVYIALNKPRGVVCTTERHIEGNIVDFVNYPERIFPIGRLDKDS
                     EGLILLTNDGGIVNKVLRSTNNNEKEYVVTVDKDITDEFLKKMSKGVKIYNPVRNEYA
                     ITNPCKVVKINNRAFKIILTQGLNRQIRRMCTKLSYKVVNLKRIRIMNIELGTLPVGK
                     WRHLTDDEVENLFRIISSEEPVQKY"
     gene            3945..5240
                     /locus_tag="PLI11_03165"
     CDS             3945..5240
                     /locus_tag="PLI11_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51894.1"
                     /translation="MFVKGKKTIIFVLILAILFSLTSGITASSTKTLVYYDGFEDNKA
                     NPKAIFRFNAWHKIENEQLYMGFNINDNDWGEAAEAYIANQRLDFDEENKKYEYVVKF
                     NSNGFRNDLWYSCMVGIRVAGNDKNDFKPGDANSGLYVGIAHTNKAVVYHGSGGWPGG
                     AFSIDIPANFAETNTLYIVDDNDTVRYYMDTPEEPRVLFLSIKISGDMLTAYDKDDNV
                     IYEAANNLKGQTGYFKIFSHSTKTIVDSIEIYSVDDSVPTAPEAAASYSGSQIRSTLV
                     PFRTKSGEIVDSDLRQGLRFCFDVMGVGEVFDYEGKEYTVKDLGGLVILKDKLSDPNI
                     MVLEQVGKIDGLVAQPEMIDVDVNGNMYKSIYIYNIPDTAKETIILARVWLECEDSDG
                     NIIYLYTSVQENSLSRVYQEIVENNEADSLDPAVRSWFE"
     gene            complement(5357..>5946)
                     /locus_tag="PLI11_03170"
     CDS             complement(5357..>5946)
                     /locus_tag="PLI11_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPZ51895.1"
                     /translation="PMTKVQFNKEFLYRYFSHDVVDNVLTCFNNKQIRLKGEKLSLIR
                     TKLQNLKESENEDDRIPAFIHLYEILFLLSREKKGVIRDASHNPYVYKIINYINKYYS
                     TIFTVDDIAEYCGVSKHHLCRVFKENMNMTIISFLNQAKVNNACKELVETDNQILQIA
                     LNNGFNSASYFNYVFKKQTGISPIEYRNIHTKKSK"
BASE COUNT         1887 a          934 c         1373 g         1742 t
ORIGIN      
        1 cgcgaactaa gggatgacga ttatgtgctt cctcctgact ttgacaacat gacaacatcc
       61 atggagtcat ttgataaaca atggatgtat catgcagtca gtcaaaccat aatagctaac
      121 actatcctaa gacagttgga gtttgtggac aaggataaaa tagggcttac gggtatttcc
      181 tggggaagtg taatagcttc aattgcaatt ggttatgatg acagatttct ctttgccata
      241 cctgtgtatg gaagcggata cctttatgag agtcttgcct ggattaaaga caattttaac
      301 tatcctggaa caaaagaaat ctgggaacct tcacatagat tgaatagtgt aaaaatgcca
      361 gttttatggc tctgttggac tgatgactct tgtttttcca tcaattccaa cgacaaatcc
      421 tttgcagata cgaaaaacgg cattctttca atgaagcagg atatgcagca cggccatatt
      481 gaagggtgga ttgctcctga gatttacagg tttgctgatt gtattgtaaa aggtacaaag
      541 ccctttgcga catgtattga aaagattggg actgaaagaa atgtttcttt ttatatggat
      601 atacctgagg atgcggatga agttactgca aaagcatatt atattaaaga gaaactcagt
      661 tattcacgca atggtaggta taaattggag tggtgtgata caatagatca ggagtggttt
      721 accgtggact gcagtgtgga gggagatgtg attcatgtaa agcttcctga agatgcacat
      781 agttactaca ttgagcttac aactcatgta tgcggtaacc cttatatcac aacatcccgg
      841 tttttaactc tggatgtgaa aagttgatat tttagctcat ttgtgttaat atttcacaaa
      901 ataaaataaa acttaaaatg cgttgaagag aagagtagga agcaatagct tttacagaga
      961 acccaggggg ctgagaatgg gcaaagcgac ggctttcgaa catggcctcg gagcagcgta
     1021 ccgagaagaa ttattctttt aggctacgac gggatctccc gttatagaga tagagtatcg
     1081 gtaaacgata tgatttaccc gtactagaag aggtccattt cgtgaggaat ggatgaattt
     1141 gggtggtacc acgaagcttg gctctcgtcc cagaaattat atttctgggc gggagctttt
     1201 ttaatatatt ttgaaatggg ggtttacaat gaaaaacata gttattggcg gtgcgtggcc
     1261 ttatgccaat ggttcccttc acataggaca tattgcagcc ttgctgcctg gtgatgttct
     1321 tgcaagatat tataggttga aaggtgacag ggtgttttat gtttcaggaa gtgattgtca
     1381 cggaacaccg gtgacaataa gggcgaggca ggaaggccgt acaccggaag agatatctgg
     1441 attttatcat caagagtttg tgcgtgtatt tgataagctt ggttttagtt atgatttgta
     1501 taccaagacc acggataaag ctcannnnnn nnnnagctca taaggaattt gtgacggagt
     1561 ttcacaaaaa gctatatgag agtgagtttg tgtacgaaaa gagtgcacct gaagcttttt
     1621 gcaagcaatg tggaaaggtt ctaactgaca ggcttgttgt tgggttgtgt ccgaattgca
     1681 aaaaggagac aagatcagac cagtgtgata attgcagtgc tgtattggaa tcacacactg
     1741 tgattgatcc tgtttgctcg gagtgtggaa atagtgtggt ctttcgtgaa accaaacagc
     1801 tgtatcttgc catatcgaag ctgaaaaagc atctttctga ctatctgcta tcaaaaccac
     1861 attggagaaa gaatgcctat gccttcacca aaagatatct ggatgaagga ctgagggaca
     1921 gagctataac caggagcatt gactggggaa tagatgtccc aagattaggg tttgaggata
     1981 agaagatata catttgggcg gagaatgtgc ttggttattt gtcagccagc aaacaggtgg
     2041 cagatgaaag gggtgttgat tttaaggaag tgtggggaag caatgcacgc cattattaca
     2101 tccacggcaa ggataatata ccctttcata cgataattct tcctgcactg ctgattgctc
     2161 atggtgacaa cttgagacta cctgacgata ttgtttcaag cgaatatttg acattggaag
     2221 gcagaaaaat atcaacaagt caaaactggg cggtatggtt aaaggatatt gttgacaggt
     2281 acaattctga ttctttgaga tactttttcc tagcaaatgg tcctgagaaa agggattcgg
     2341 acttttcatg gcatgagttt gcagagagga acaattcgga tcttgttggg gtatatggca
     2401 actttgtcaa caggacgctg gcttttatag ttaagtataa taacggagtt gttccttgcg
     2461 gggccttgga aaaggatatc gaaaaaaaga ttaacgatgc atattcagag gcaggcagtt
     2521 tgcttgaaga cggtcagttt aaaggagcac ttgaggttat ttttgacctt gtgcgctttg
     2581 gcaacaaata ctacgacaca atggagccgt ggaaaacgag acacggtgat aaggaaaagt
     2641 gttttaatac actgtataac tgtgtgcaga taattgcaaa tcttgctgtc ttgctggagc
     2701 ccatccttcc tttttcctcg cagaaagcca ttgactggct tggtttggat agagcttgga
     2761 aacctcaatt tgtcaaggaa ggccaccagc ttaccgatat ctctattctt tttgaaagga
     2821 tagacaagga tgtaatagaa gaggaaagga acagactaaa aataaccaat acgtaacaca
     2881 taattaacca aaatacaatt gatttatata tttatgtgga atatattgtt ctggcaacct
     2941 ccccgtaatg gctgcatgcc ctaaagggga gtttttttgt tcaagtcaca actggcatag
     3001 ataaaacact agttgggata atatatgtga ttttaagaag aatgaaaagg agaacatatg
     3061 agattaaata gatatattag ttcaactggt ctatgctcaa gaaggcaggc tgatgaactt
     3121 attaaacagg ggaaggtcaa ggtaaataat acagttgcag taattggtac tgatgtgagt
     3181 ccggatgatg tggtagaggt aaatgggaaa cttcttaaag gtaaaagcga atatgtatat
     3241 atcgcactga acaaaccaag gggtgttgta tgcacaacgg aaagacatat agagggaaac
     3301 attgttgatt ttgtaaacta tcctgaacgc atcttcccta taggaagact cgacaaggat
     3361 tccgaagggt tgattcttct taccaatgac ggtggaatag ttaacaaggt tttaagaagc
     3421 accaacaata atgaaaagga gtatgttgtg accgttgaca aggatataac ggatgagttc
     3481 ctgaaaaaaa tgtcaaaggg cgttaagata tacaatccag ttagaaatga gtatgcgata
     3541 accaatccct gcaaggttgt gaagattaat aacagagcat ttaaaataat tcttacacaa
     3601 ggattgaaca ggcaaataag gagaatgtgt accaaactga gttacaaggt tgtcaatttg
     3661 aaacgcatta gaattatgaa tatagagctt ggcaccttgc ctgtaggcaa atggagacat
     3721 ttaactgatg atgaggttga aaatctattt agaataataa gctcagaaga gccggttcag
     3781 aaatattgat aagtcagtat tatcgtatca cattaattct tattgttttt gctatataac
     3841 atagaaaaaa atcaatatat ctaaatctcc ctcaatatat cagaagacac atagaaatct
     3901 ttttgataat ctttgcttac cagataaaaa ggagagattt ggatatgttt gtaaaaggga
     3961 agaagactat catatttgtt ttaatcctgg ctatattatt cagtttgaca agcggcataa
     4021 ctgcctcgtc aacaaaaaca ctggtgtatt atgatgggtt tgaggataat aaagccaatc
     4081 caaaggcaat attccgtttt aatgcatggc acaagataga aaatgagcaa ttgtatatgg
     4141 gttttaacat caatgacaac gactggggag aagctgctga agcatatatt gcaaaccaaa
     4201 ggttggattt tgatgaagag aataagaaat atgagtatgt agtcaaattc aacagtaatg
     4261 gctttagaaa tgacctttgg tactcatgca tggtaggcat tagggttgca ggtaacgaca
     4321 agaacgattt taaaccagga gatgccaact ctggactgta tgtaggaatt gcccacacca
     4381 ataaagcagt tgtctatcat ggtagtggcg gttggcctgg aggagctttt agtattgaca
     4441 ttccagccaa ctttgcggag actaatacac tttacatagt tgatgacaat gacactgtca
     4501 ggtactacat ggatacacct gaagaaccca gggtgttgtt tctgtcaatt aaaatatcgg
     4561 gagacatgct tactgcatat gataaggacg acaatgttat ttatgaagcg gcaaacaatc
     4621 tgaaggggca gacggggtac tttaagattt tcagccatag tacaaaaacc attgttgatt
     4681 ccatagagat atacagtgtg gatgattctg ttccaactgc tccggaagca gctgcatcat
     4741 attcaggttc ccagataaga tcaacactgg tacccttcag aaccaaaagt ggcgagatag
     4801 tagatagtga tttgagacag ggtctgagat tctgctttga tgtaatgggt gttggtgaag
     4861 tatttgacta tgagggtaaa gagtacactg tcaaagattt aggtggactt gtcatactga
     4921 aagataaatt gtcagatcca aatataatgg ttttggaaca ggttggcaag attgatggat
     4981 tggttgcaca gccggagatg attgatgttg atgtgaatgg taatatgtac aaatccattt
     5041 acatatataa catacctgat actgcaaagg aaacaattat tctggcgaga gtatggcttg
     5101 aatgtgaaga ttccgatgga aatataattt atttatatac ctctgttcag gagaattcgc
     5161 tttcaagggt ataccaggaa atagttgaaa acaacgaagc agacagcctt gatccggctg
     5221 taaggtcatg gtttgagtaa ggaggagctt atgaaaaaga tatacgagga tccgcaaatg
     5281 gatgttgtga tatttgaaac ggaagaggaa gttacagtaa gtggtctgga tttcataggt
     5341 gttgacttcg aagaccttat ttagatttct ttgtatgtat gttcctgtac tcaatcggtg
     5401 atatacctgt ctgcttttta aatacataat tgaagtagga ggctgaattg aaaccgttat
     5461 tgagtgcaat ctgcaggatt tgattatccg tttcaacaag ttccttgcat gcattgttta
     5521 cttttgcttg attaaggaat gaaattatgg tcatattcat attctcttta aatacacgac
     5581 ataaatgatg ttttgatact ccacaatatt ctgcgatgtc atcgacagta aaaattgtgg
     5641 agtaatattt atttatgtag ttaataattt tgtacacata tgggttgtga cttgcatccc
     5701 taataactcc ctttttctct cttgacagca agaacagaat ctcatacaga tgaatgaagg
     5761 ccggtattct gtcatcctca ttttcagact ctttaagatt ctgcagtttt gtccttatta
     5821 gcgagagttt ttcacctttt aatcgtattt gcttgttgtt aaagcacgta agcacattgt
     5881 ccaccacatc gtgactgaag tatctgtata aaaattcctt gttgaactgc acctttgtca
     5941 tgggca
//
LOCUS       DAONVY010000075         5920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10002_length_5920_cov_3.666326, whole genome shotgun sequence.
ACCESSION   DAONVY010000075 DAONVY010000000
VERSION     DAONVY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_5920_cov_3.666326"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLI11_03175"
     CDS             complement(<1..172)
                     /locus_tag="PLI11_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPZ51896.1"
                     /translation="MKEKIFQGSGVAIVTPFSEDGINYEKLGEMIEFQISEGTDAIII
                     CGTTGEASTMPDG"
     gene            complement(204..1274)
                     /gene="asd"
                     /locus_tag="PLI11_03180"
     CDS             complement(204..1274)
                     /gene="asd"
                     /locus_tag="PLI11_03180"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPZ51897.1"
                     /translation="MKRRVGVLGATGMVGQRFLSLLDNHPWFDVKIVAASARSAGKTY
                     EEAVGGRWKLKTPMPEYVKNMVVKDANDVDSIASEVDFVFCAVDMKKEEIKELEERYA
                     KAECPVVSNNSAHRFTPDVPMIVPEINPEHAAIIPWQRKRLGTKRGFISVKSNCSLQS
                     YIPALHPLRDFGLDKVLVTTYQAISGAGKDFDSWPEMIDNVIPYIGGEEEKSEVEPLK
                     LFGTVQNGEIVLAKSPSITAQCIRVPVLDGHMACVFASFDTKPQIDEVIDIWNNFKGR
                     AQELELPSAPRQFLKYYTEENRPQTRLDRDYENGMGVTIGRLREDTHYDIKFVCLSHN
                     TVRGAAGGAILMAELLCKEGYM"
     gene            complement(1327..1737)
                     /gene="yajC"
                     /locus_tag="PLI11_03185"
     CDS             complement(1327..1737)
                     /gene="yajC"
                     /locus_tag="PLI11_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPZ51898.1"
                     /translation="MKKIKKILFTTAIMAGTLMSFVVSAAEESPAADQDSAKGGLLGG
                     FLPIIAMYALLFGAMYFILIRPQRKRQKKEDELRNSVILGDNITTIGGVTGRVVQIKD
                     DEITIESSIDRTLLTFKKWAIRDVQKLETDDEEK"
     gene            complement(1770..2885)
                     /gene="tgt"
                     /locus_tag="PLI11_03190"
     CDS             complement(1770..2885)
                     /gene="tgt"
                     /locus_tag="PLI11_03190"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPZ51899.1"
                     /translation="MPAVRYELIKTCKQSGARLGKLHTPHGTFDTPMFMPVGTQGTVK
                     GISPEELKEMGAGVILSNTYHLYLRPGHELVKEAGGLHKFMNWDRGILTDSGGFQVFS
                     LSKLRKITEEGVHFSSHLDGSKHFLSPERVMEIENALGADIIMAFDECTPYPSSYEYT
                     KDSANRTARWAERCLESHKNTDKQSLFGIVQGGMYADLRQMSARDIVSLGFDGYAIGG
                     LSVGEPGPLMYEMLEATVPCLPDDKARYLMGVGSPDYLIEGSIRGIDMFDCVLPTRIG
                     RNGTAMTSMGRVIVRDARYAKDFSPIDPECDCYACKNYTRAYIRHLVKAGEMFGLRLV
                     TIHNIYFLLNLMKSIRQAIINDRLLDFKVEFFEKYGL"
     gene            complement(2948..3973)
                     /gene="queA"
                     /locus_tag="PLI11_03195"
     CDS             complement(2948..3973)
                     /gene="queA"
                     /locus_tag="PLI11_03195"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPZ51900.1"
                     /translation="MKVSDFYYDLPEELIAQKPLEDRAASRLMVLDKKTGQTAHCVFR
                     DILKYLKKGDCLVLNDTKVIPARLYGRKENQQSRIEFVLIKKHTQTIWEVIVRPGKKV
                     RPGSCIVFGEGKLKAYVREILENGNRIVEFEYDGIFEEVLDEVGLIPLPPYITEKLDD
                     PDRYQTVYAREKGSSAAPTAGLHFTDELLEEIREMGVDIAYVTLHVGLGTFRPVKVAN
                     VEDHYMHTEYYEIKEENAKIINDARESGGRIFCVGTTSCRVLESVSDENGFVKADTGE
                     TDIFIYPGYKFKIVDALITNFHLPESTLLMLVSSLAGRENILNAYKEAVNKRYRFFSF
                     GDAMLII"
     gene            complement(3963..5795)
                     /locus_tag="PLI11_03200"
     CDS             complement(3963..5795)
                     /locus_tag="PLI11_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPZ51901.1"
                     /translation="MARKATSFILCIALITGIMLYPTHAESLEYNIPEYIRVALKYEG
                     TALDFVYVRSDTGGTVGIVLDNNHIEMNIFSASDTVEVVSDSNYHLIIGEEFDTYDEV
                     KAFIEVLEEHAGYDFSIPLFPLYKGKWVAAASIYTDEPVMEETTADNGIDEYAILYET
                     FALADLIDTPEGKEEGPSWPVIPYKTERFAMRAEIEGTPLFAVSDPANAVSVRVKPQD
                     TGNPNVVPLLRLYELSQPEHLRFRSRYRGVFDLHRQEMQKKDRFVVVNKLKLEEYLYS
                     VVHMEMSAGYDHQYNERIEALKVQAIVARSFAVKMVELKSYQKYRADIENTTNTQVYP
                     GYTHVNGNQGEWNNTNSAVDQTRGQVLMYDGKLAVNIYYHSNSGGYTEHPIYVWYRGG
                     SYPPYVVSKPDPYTELAPRVWTNTFTGKSIQQTIRKYVEDITMTSDGSFTDIGNVKYI
                     NVVERSTAGRTTETLIEGTNASFVLRNEQNRWVYSLMGNMYNYEVDDKIRIVEQRDGD
                     KKFLSERARKVFEGEYFTKEYLADKYGVVTENGHVYERIKVYDSDNQTIIVNGRGSGH
                     GVGMSQHGAQQMSKLGLTSEEIINFYFPGVQISKRKQEIDINEG"
BASE COUNT         1497 a         1389 c         1098 g         1936 t
ORIGIN      
        1 caccgtcggg cattgtggag gcttcgcctg tggttccgca tattattatt gcatcggtac
       61 cctcgcttat ttgaaattca atcatctctc ccagcttttc atagttgatt ccatcttctg
      121 aaaaaggagt gactattgca actccggaac cttgaaatat tttttctttc ataaaagaca
      181 tcccctttta atgtttacaa agattacata tagccttcct tgcataggag ctcagccatt
      241 agaattgcac caccagcggc accccttacc gtattgtggg agagacatac gaactttata
      301 tcataatggg tgtcttccct gagacgacct attgttacgc ccataccgtt ttcatagtca
      361 cggtcgagtc ttgtttgagg tctgttttct tcggtgtaat atttcaggaa ctggcggggc
      421 gcacttggca gttcgagctc ttgcgcacgt cctttaaagt tattccatat atcaattact
      481 tcatctatct gtggctttgt gtcaaatgat gcaaaaacac aagccatatg tccgtcaagc
      541 actggaactc tgatacattg ggcagttatg gaaggacttt ttgccaaaac tatttcacca
      601 ttctgaacgg ttccaaatag tttcaaaggc tcaacttcag acttttcttc ctctcctcca
      661 atgtatggaa ttacattgtc tatcatttcg ggccatgaat caaaatcctt tcctgcaccg
      721 gaaatagcct gataagtggt taccaaaacc ttgtcaagcc caaagtctct taatggatga
      781 agggctggaa tatagctttg gagagagcag tttgacttca cacttataaa tcctcttttg
      841 gttcccaaac gttttctctg ccaggggatt atggctgcat gctcaggatt gatttctgga
      901 actatcattg gcacgtcagg agtaaacctg tgagcgctgt tgtttgacac aacagggcat
      961 tcagcctttg catacctttc ctcaagttcc tttatctctt cctttttcat atcaacagca
     1021 caaaaaacaa aatccacttc gctggcaata ctgtcaacgt cattagcatc cttaactacc
     1081 atgttcttta catactcagg catgggagtc ttcagcttcc atcttccgcc cactgcttct
     1141 tcatatgttt ttccagcaga tcttgcacta gcggctacta ttttcacatc gaaccatgga
     1201 tgattatcca ataggcttaa gaaacgttga ccaaccatac cggttgctcc gagtactccg
     1261 actcttcttt tcataaagaa ttaacctccc gtatattagt atatgagatt tgatttctat
     1321 ttgcttctat ttctcttcat catcggtttc tagtttttga acatctctta ttgcccattt
     1381 cttgaatgtc aaaagcgttc tgtcaatgga tgattcgata gttatctcat cgtcttttat
     1441 ttgcacaaca cgtcctgtaa caccgccgat ggttgtgatg ttgtcgccga gaataacact
     1501 gttgcgaagt tcatcttcct tcttctgtct cttcctttga ggacgaatca gtatgaaata
     1561 catggctcca aacaaaagag catacatcgc aatgataggg agaaagcctc ctaaaaggcc
     1621 gccttttgct gaatcctgat ccgcagcggg gctttcttca gcagccgaaa caacaaagct
     1681 catcaatgtc cccgccatta ttgctgttgt gaataatatc tttttaatct ttttcatata
     1741 ttaacctcca agttatctat ttcatctatt taaaggccgt atttttcaaa gaattccact
     1801 ttgaaatcaa gcagcctgtc attaattata gcttgtctga tagatttcat caagtttaac
     1861 agaaaataaa tgttgtggat tgttacaagt cttaacccaa acatttctcc tgcttttaca
     1921 agatgtctga tatatgctct tgtatagttt ttgcatgcat aacagtcgca ttccggatcg
     1981 atcggagaga agtcttttgc gtaccttgca tcccttacaa taacccttcc cattgaagtc
     2041 attgcggttc cgttcctgcc gattctggta ggcaatacgc aatcaaacat gtcgattcct
     2101 ctgattgaac cttcaataag gtagtcagga cttccaacac ccataagata tcgggccttg
     2161 tcgtcaggca aacaggggac tgttgcctca agcatttcat acataagagg tccgggttca
     2221 ccaacactga gaccaccgat ggcgtatccg tcaaaaccca gagatacaat atccctggca
     2281 gacatctgcc tcaaatccgc atacattcct ccctgtacaa taccaaacaa ggactgcttg
     2341 tcggtgttct tgtggctttc caaacacctt tcagcccatc ttgccgttct gtttgcggaa
     2401 tctttagtgt actcataaga agaaggatag ggcgtacatt cgtcaaacgc cattattatg
     2461 tccgctccca gtgcattctc aatttccatc actctttcag gggacaaaaa atgtttagag
     2521 ccgtccaaat gggaactgaa gtggactccc tcttccgtta ttttcctcag tttgctgagt
     2581 gaaaaaacct gaaatccacc gctatcggtc agtatgcctc tatcccagtt catgaacttg
     2641 tgaagtcctc ctgcttcttt gaccagttca tgaccaggtc ttaaatacag gtgataagtg
     2701 ttggaaagaa taacacctgc acccatttcc ttaagttctt ctggtgaaat gcctttgacc
     2761 gtgccttgtg tacctacagg catgaacata ggtgtatcaa aagttccgtg gggtgtatgc
     2821 agttttccaa gccttgctcc tgattgtttg caagttttaa tcaactcgta tctgacagct
     2881 ggcatctgtt actcctttaa tttttttatg aatgtaaaat attatcatat ttatgaagaa
     2941 aaatcaatta gataataagc atggcatctc cgaaactgaa gaatcggtac ctcttattca
     3001 ctgcttcctt gtaagcattt aatatgtttt ccctgcctgc aaggctgcta accagcatta
     3061 acagggtgga ttcgggaagg tggaaatttg ttatcaatgc atcgactatt ttaaatttgt
     3121 atccagggta aatgaaaata tcggtttctc cagtatcagc cttaacaaaa ccgttttcat
     3181 ctgaaacgga ctcaagaacc ctgcaggagg tggttccgac acagaaaatc ctcccgcctg
     3241 actctctggc atcgttgatt atctttgcat tctcctcttt tatttcataa tattccgtgt
     3301 gcatgtagtg gtcttccaca tttgccacct ttacaggtct gaatgtgcca aggcctacat
     3361 gcagtgtgac ataggcaata tcaacgccca tttccctgat ttcctcaagg agttcatcag
     3421 tgaaatgaag gcctgcagta ggtgccgcac tggagccttt ttcccttgcg tacactgtct
     3481 gatacctgtc agggtcatca agtttttctg tgatatatgg ggggagggga ataagtccta
     3541 cctcgtccaa aacttcttca aaaataccgt cgtattcaaa ttctacaatc ctattgccgt
     3601 tttcgagaat ctcacgaacg taggctttta gttttccttc tccaaataca atgcaagaac
     3661 ccggccttac tttctttccg ggtcttacga ttacctccca tatggtttga gtgtgttttt
     3721 ttatcagaac aaactcaatc ctggattgtt gattttcttt tctgccataa agtcttgccg
     3781 gtattacctt tgtgtcgtta agaaccagac agtcaccctt ttttaaatac tttagtatat
     3841 ctctgaaaac acagtgggct gtctgtccgg ttttcttgtc cagaaccatc aaccttgagg
     3901 ccgccctgtc ttcaagaggt ttctgggcta tcaactcctc cggcagatcg taataaaagt
     3961 cgctaacctt catttatatc aatttcctgc tttctcttac tgatttgaac acctgggaag
     4021 tagaaattta taatctcctc gctggtcaat cccagtttgc tcatctgctg ggctccgtgc
     4081 tgactcattc ccacgccatg tccggaacct cttccattaa caattattgt ttgattgtcg
     4141 gaatcatata cctttattct ttcataaaca tgaccgtttt cagtaactac gccatatttg
     4201 tcagccaggt attctttagt aaagtattct ccttcaaaca cctttctggc tctttcgctt
     4261 aaaaactttt tatctccatc cctttgctcg acaatcctta tcttgtcatc aacctcgtaa
     4321 ttgtacatgt tacccatgag tgaatacacc catctgtttt gttcatttct cagtacaaag
     4381 cttgcattgg ttccttcaat gagggtttca gttgtccttc ccgctgtaga ccgctcaaca
     4441 acattaatgt attttacatt tcctatgtca gtaaacgaac catcggaagt cattgtaata
     4501 tcttcaacat attttctaat ggtctgctgt atgctctttc ctgtaaaagt gttggtccaa
     4561 accctgggtg caagttcggt atatgggtca ggttttgaaa caacgtaagg agggtagctt
     4621 ccacctctgt accaaacata aatgggatgt tctgtatagc cgccactgtt tgagtgatag
     4681 tatatattca cagcaagttt tccgtcatac atgaggactt gtccccttgt ttgatccact
     4741 gcggagtttg tgttgttcca ctcgccctga ttgccattta catgggtgta tccgggataa
     4801 acttgtgtgt ttgttgtgtt ctctatatct gccctgtatt tctgatagct ctttaactcc
     4861 accattttaa ctgcaaagga tcttgcaacg atggcttgca cttttaatgc ctctattctt
     4921 tcattgtact gatggtcgta gcctgcagac atttccatat gaacaacgct gtagagatac
     4981 tcttctagtt tgagtttgtt cacaacaaca aacctgtctt ttttctgcat ctcctgtcgg
     5041 tgaaggtcaa acacacccct gtatcgtgag cggaatctta aatgttccgg ctgtgacagt
     5101 tcgtatagtc ttaacagagg cacaacattc gggttgcctg tatcctgggg ttttaccctt
     5161 acactgacag cattggccgg gtccgataca gcaaaaaggg gagtgccttc tatttctgct
     5221 ctcatggcaa atctttcagt tttgtaaggt atcacaggcc aagatggacc ttcttctttt
     5281 ccttccggtg tgtcaatcag gtcggcaagg gcaaaggtct catacaatat cgcatactcg
     5341 tcaataccgt tgtctgctgt tgtttcctcc atgactggtt cgtctgtgta aattgaagcg
     5401 gcagctaccc attttccctt gtaaaggggg aaaagcggga tcgaaaagtc ataaccggca
     5461 tgctcttcca aaacctcgat gaatgccttt acttcatcat aggtgtcgaa ttcctcgcca
     5521 attatgaggt gataattgct gtcgctgaca acttccacag tgtcactggc actgaatatg
     5581 ttcatctcta tgtgattgtt atcaagaaca atacctacag tacctcctgt atccgaacga
     5641 acgtatacga aatccagtgc ggtgccctca tacttgagtg caactcttat gtattctgga
     5701 atattgtact caagactttc cgcatgtgtt ggatacagca ttattcctgt aattaatgca
     5761 atgcatagta tgaaacttgt tgcttttctt gccatgacaa acaaaccttt cctggtatat
     5821 taaaagcccc aaatggtttt aaaataatct agcgcttcat tggtcaaatc tgatatgtca
     5881 agattattgt agttcttttc atttaccttg actttgtact
//
LOCUS       DAONVY010000076         5811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10223_length_5811_cov_3.967165, whole genome shotgun sequence.
ACCESSION   DAONVY010000076 DAONVY010000000
VERSION     DAONVY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10223_length_5811_cov_3.967165"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLI11_03205"
     CDS             complement(<1..451)
                     /locus_tag="PLI11_03205"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HPZ51902.1"
                     /translation="MSKMPVLFVGHGSPMNAIEDNQFSRSWKEIARKIPKPEAILSVS
                     AHWYTRGTKINNEENPKTIYDMYGFPRPLYEIVYDCPGSPTVAKVVKALVTKDSEYDN
                     TWGIDHGTWSVLVHMYPDRDIPVLQLSVDRNAPPEVHYMIGKELGALR"
     gene            complement(484..876)
                     /locus_tag="PLI11_03210"
     CDS             complement(484..876)
                     /locus_tag="PLI11_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51903.1"
                     /translation="MMIVYPEHCGNSPKKILLIEIYKAITEKDDDFILEHVAEDVMLD
                     VVGVKKLLGKKEVLNSIKNLVDMDLSKIEVANVITHGNVAAVYGCYHFAKGNFEFCDV
                     YVFGSFGKKAKIKEIKSFLINPDKHNVI"
     gene            complement(946..2427)
                     /locus_tag="PLI11_03215"
     CDS             complement(946..2427)
                     /locus_tag="PLI11_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HPZ51904.1"
                     /translation="MSLINVSNLTFSYDGSYDNIFEDVSFQIDTDWKLGFIGRNGRGK
                     TTFLKLLMGEYEYSGSISASVEFEYFPYEVEDKTRFTIDVMNEICPECMEWEKVKELS
                     LLDVEEDILYRPFATLSNGEQTKVLLASMFLRRNCFLLLDEPTNHLDIDARRVLVNYL
                     KNKKGFILVSHDRNLLDTCIDHVLSINKTNIEVQKGNFSSWWENKMRQDNFEIAENRK
                     LVREIKRLSEATRRSAVWAHRVEKSKYGKDDSGIKLDKGYVGHKSAKAMKRAKNIEAR
                     KMKAISQKSELLHNIEEYDDLKITPLEFYKRTLVEAKDLAIFYGDRIVFSNLNFAINA
                     GDRVALAGRNGSGKTSILKLIVGEGIQYSGSLIVASGLKVSYISQDTSWLYGNLSDFA
                     YENGIDETQFKTILRKLDFGREQFDKDIADFSAGQKKKVLLAKSLCEQAHLYVWDEPL
                     NYIDVFSRMQIEKLILEYCPTLLFVEHDEAFRNNISTKVVEIE"
     gene            complement(2724..3755)
                     /gene="mcrC"
                     /locus_tag="PLI11_03220"
     CDS             complement(2724..3755)
                     /gene="mcrC"
                     /locus_tag="PLI11_03220"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019002570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HPZ51905.1"
                     /translation="MIPIRNIYYMLSYAFSVLNKQGYRELATESFKNAAELCAAILHI
                     GISHQIKRGLGRNYITETESVSSLRGKIDLSQSIKTQSILKNQMVCQYDDFSVNTYMN
                     RILKTTMMLLMHADISRIRKKELRKVLVYFDAVDGLDVRSINWNLQFNRNNHTYSMLV
                     SICYLVVKGLLQTQSDGKTRLMEFDDEKNMARLYEKFILEYYRKEHPYLDVCSSRIMW
                     QLDDDMNAMLPIMQTDIMLSKDEKILIIEAKYYARITRSRFETKTLHSNNLYQLFAYV
                     KNKEAELCGRPHEVSGMLLYARTDEEILPDYDYRMSGNKISVRTLDLGCSFEKIKAQL
                     DSIAYDYFS"
     gene            complement(3752..>5811)
                     /locus_tag="PLI11_03225"
     CDS             complement(3752..>5811)
                     /locus_tag="PLI11_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ51906.1"
                     /translation="LIEKVRRIYEVTGIAMPTLERGNQIVDIDPFTVIGLFNKGLGEN
                     SRKRVIAAIADIFGLKSPIPTDFSGIPVVNPQNATFYYFLGDRGDNDINELWELFEYA
                     LQYVENPTGDKRETVGRYFDLAMSKKGIGNSKLTIGLYWIAPYDFISLDKHNVQYIYK
                     SGEFPEEHVASLPEIEGKIPSGKYFAIVKKLKEYALSTDKYKSFPELSHNAWLFSKEK
                     GRAIRDSKGDGLADEDVSTVHYWIYSPGDNASMWDEFYEKGIMGIGWDEIGDLLQYSS
                     KDEMQKAMKERIGPNFSFRNSALATWQFVHEMKPGDIVFAKKGQNQIVGWGIVETAYY
                     YDPSENEEYPNLRKVKWKDKGEWEHPGKAVTKTLTDITQYTQYVEKLLALFGKDTSDE
                     EIEAVKVEYPTYTENDFLNEVYMEPDTYYSLVDRVKNKMNVILQGPPGVGKTYAAKRL
                     AYSIMGIKDPERVMMIQFHQSYSYEDFIEGYRPVKGGGFDIEKGVFYEFCNKAELDPE
                     NEYFFIIDEINRGNISKIFGELFMLIESDKRGNTIRLLYSGDGFMVPKNLYIIGTMNT
                     ADRSLAMLDYALRRRFAFFNMKPAFDYDGFREYRIKLKSEKFDELINCVERLNSEIVA
                     DDSLGEGFCIGHSYFCGKDEIDDSVLSGIVEYELIPLLKEYWYDDPQKVKDWAESLRR
                     AIK"
BASE COUNT         1663 a         1349 c          910 g         1889 t
ORIGIN      
        1 ttctcaaagc ccccagctcc ttgccaatca tgtaatgcac ttccggaggc gcatttctat
       61 caacacttaa ttgaagcacg ggtatgtctc tatcgggata catgtgtacc agtaccgacc
      121 aggtcccatg gtctattccc caagtgttgt catactcgct gtcctttgta accagtgctt
      181 tgacaacctt ggctactgtg ggagagccgg gacaatcata cacaatctca taaagtggcc
      241 ttggaaaacc atacatgtcg taaatggtct ttggattttc ctcattgttt atctttgtac
      301 ctcttgtgta ccagtgggct gaaacggaca aaatcgcctc aggttttgga atcttccttg
      361 caatttcttt ccacgacctt gaaaactggt tgtcttcaat tgcattcatg ggtgaaccat
      421 gaccaacaaa caaaaccggc atcttcgaca tatatctcac agtcccttct tcatttcgtt
      481 ttactatatc acattatgct tgtccggatt tatcagaaaa ctctttatct cctttatctt
      541 ggccttcttg ccaaagctgc caaatacata tacatcacaa aactcaaagt ttcctttggc
      601 aaaatgatag catccgtaga cagcggcaac atttccatgg gtaataacat tcgcaacttc
      661 tattttgctc aaatccatgt cgacaaggtt ctttatactg tttaaaactt ctttcttgcc
      721 caaaagcttt ttaacaccaa ccacatccaa catgacatct tcagcaacat gctcaagtat
      781 gaagtcatca tctttttccg ttatggcttt gtatatttct ataagcaaaa tcttcttggg
      841 tgaattaccg cagtgctccg gataaactat catcataaca cctaactcca ttttttgata
      901 atacatatac attaccaaat tttaggccaa catgcaaaag aataattact caatttctac
      961 aacttttgtg ctgatgttgt ttctaaaagc ctcgtcatgc tcaacaaaca aaagtgtcgg
     1021 acaatactca agaatcaact tctctatctg catgcgtgag aacacgtcaa tgtagtttaa
     1081 aggttcatcc cagacataaa ggtgtgcctg ctcgcaaaga cttttagcaa gaagcacttt
     1141 ctttttctga ccagcgctaa agtccgctat gtctttgtca aactgttctc ttccaaagtc
     1201 gagcttcctg agtattgttt taaactgggt ttcatcaatc ccattttcat atgcaaaatc
     1261 agaaagattt ccgtacagcc atgatgtatc ctgtgaaatg tatgaaactt tcagtccgct
     1321 ggcaactatc aagctgcctg aatattgaat tccctcccca acaattagtt taagaatact
     1381 ggttttgcca cttccatttc tgcctgcaag ggcaactctg tcgcctgcat ttatggcaaa
     1441 gttcagattg ctaaaaacaa tcctgtcgcc atagaaaatg gcaaggtcct ttgcttcaac
     1501 caaggttctt ttataaaatt caagaggcgt tatcttaagg tcgtcgtatt cttcaatatt
     1561 atgaagaagc tctgactttt gactgattgc cttcattttt cttgcctcaa tattttttgc
     1621 cctcttcatc gcctttgctg acttgtgtcc tacataacct ttatcaagct ttataccgga
     1681 atcatctttc ccatacttgc ttttttccac ccgatgagcc caaacagcgc ttcttcttgt
     1741 agcctccgat agcctcttta tctctctcac aagctttcta ttctctgcaa tttcaaaatt
     1801 atcctgcctc atcttgttct cccaccagga agaaaaattg cctttctgca cctcgatatt
     1861 cgttttgttt atggataata catgatctat gcatgtatca agaagatttc tgtcatggga
     1921 taccaaaatg aatcccttct tgttctttag ataatttaca agcacccttc ttgcatcaat
     1981 atccagatgg tttgtaggtt cgtcaagcaa gagaaagcaa ttccttctta aaaacatgga
     2041 tgccagcaaa acctttgtct gttcgccatt agacaaggtt gcaaatggtc tgtacaaaat
     2101 atcttcttca acatccaaca aggataattc ttttaccttt tcccactcca tgcattcagg
     2161 acagatttca ttcattacat caatagtaaa acgggtttta tcctccactt catagggaaa
     2221 atattcaaac tcaactgatg cgcttatgct tcctgaatac tcatactcac ccatcaaaag
     2281 tttaagaaag gtggtttttc ctcttccatt ccttcctata aaaccaagct tccagtccgt
     2341 atctatttga aaactaacat cctcaaaaat attgtcgtaa ctcccatcgt aggaaaaagt
     2401 aaggtttgaa acgtttatta gtgacataaa aatccctcct aacaaaatca agccgcaaga
     2461 aagtcaaact tcttacggct taaatacaca aacaaccctt tttcccaaat caaaaggtac
     2521 gtttatcaag taaagaatgt tcaactttcc tgctatttac acgcaaaaac atgcggtcta
     2581 taaccgctgt gacaaagccc tatattttgt gcatacctgt tagcaagaaa agttgtacat
     2641 cctttcacct ccaattaatc caattatatc acatattcat aaaaatctac aatataattt
     2701 tcacaaatcc ggacgcaagc attttaacta aaataatcat atgcaataga atcgagttgt
     2761 gctttgattt tctcaaaaga acaccccagg tccaaagtac gaacactaat tttattccca
     2821 ctcattctgt aatcataatc tggcaatatc tcttcatccg ttcttgcata caacagcatt
     2881 ccagatactt catggggcct tccgcatagc tccgcctctt tgttctttac atatgcaaac
     2941 aactggtata ggttgttaga atgaagcgtc ttagtctcaa accggcttct ggtaattctg
     3001 gcgtagtatt tggcttcaat gatcagtatc ttttcgtctt tggaaagcat aatatcagtc
     3061 tgcatgatgg gaagcattgc attcatgtca tcatcaagct gccacataat cctggaagaa
     3121 cagacatcaa gatatggatg ctcctttctg tagtattcca ggataaattt ctcataaagt
     3181 cttgccatat tcttttcatc atcaaactcc ataagtctag ttttgccatc ggactgggtt
     3241 tgcaaaagtc ccttgaccac cagataacaa atggacacaa gcatactgta agtatgatta
     3301 ttccggttaa attgaaggtt ccagttgatg ctcctgacgt ccaacccatc aacagcatca
     3361 aaatacacaa gaactttacg aagttctttc tttctaatcc tgctgatgtc agcatgcata
     3421 agaagcatca tggtggtttt tagaattctg ttcatgtatg tattaacaga aaaatcgtca
     3481 tattgacaaa ccatctgatt ttttagtata gattgggttt ttatggactg ggacaaatcg
     3541 attttgccgc gaagagagga taccgactca gtttcagtaa tatagttccg tccaagacca
     3601 cgctttattt gatgagaaat ccctatatgc aaaatagcag cacatagttc tgcggcattc
     3661 ttaaaacttt ctgtcgccaa ttccctatac ccttgtttat tcaatacact aaatgcataa
     3721 gacagcatgt aatatatgtt ccgaatgggt atcatttaat tgctctcctc aagctctccg
     3781 cccagtcttt cactttttgt ggatcatcat accaatactc cttaagaaga ggaatcagtt
     3841 catactccac aatccccgat aaaaccgaat cgtcaatctc atctttgccg cagaaataac
     3901 tgtggccaat acagaacccc tctcctaacg aatcatctgc aacaatctcg ctattaagtc
     3961 tctcaacaca atttatgagc tcgtcaaact tttctgattt cagcttaatc cgatactctc
     4021 taaaaccatc gtaatcaaat gcaggtttca tattgaagaa agcaaatctt cttcttaatg
     4081 cgtaatccag catggcaagg cttctatccg cagtgttcat ggttccgata atgtataagt
     4141 ttttaggaac cataaatccg tctccggaat acaatagccg aattgtattt ccgcgtttgt
     4201 cgctttcaat taacatgaaa agctcgccaa agatcttgct tatgttgcct ctgttgattt
     4261 catctataat aaagaagtat tcattctccg gatccagctc cgctttgttg caaaattcat
     4321 aaaatacacc tttttctata tcaaaacctc cacccttgac aggcctgtat ccttcaataa
     4381 agtcttcgta gctgtaactt tgatggaact gtatcatcat taccctttca ggatccttga
     4441 tacccatgat ggaataggca agacgttttg ctgcatatgt cttgccaact cccggaggac
     4501 cctggagaat tacattcatc ttgtttttga cacggtcaac tagagaataa tatgtatccg
     4561 gctccatata gacttcattg agaaaatcat tttcggtgta ggtcgggtat tcaaccttaa
     4621 cagcttctat ttcttcatca ctggtatcct ttccaaacag ggctagaagt ttttcaacat
     4681 actgcgtata ttgagtaata tcagtcaagg tcttggtaac agcctttccc gggtgttccc
     4741 attcaccctt gtctttccat ttgacttttc gaagatttgg atactcttcg ttttcagaag
     4801 gatcataata gtatgcggtt tcaacaatac cccacccgac tatttgattc tgtcctttct
     4861 ttgcaaaaac aatatcacca ggtttcattt catgtacaaa ctgccatgtt gccaaggccg
     4921 aatttctaaa agaaaaatta ggacctatcc tttctttcat agctttctgc atttcgtctt
     4981 tacttgagta ttgtaacaag tcaccaatct catcccaacc aatgcccata atacctttct
     5041 cgtaaaattc atcccacatt gaagcattat caccaggtga atatatccaa taatgtactg
     5101 tgctaacatc ctcatctgca agtccgtctc ctttgctgtc acggattgct cttccttttt
     5161 cttttgaaaa cagccaagca ttatgtgata gttcgggaaa actcttgtac ttgtccgtgc
     5221 ttaatgcata ttcttttagt ttttttacaa ttgcaaagta ttttccggag ggaattttcc
     5281 cttcaatctc aggcaatgat gcaacgtgtt cttccgggaa ttcacctgat ttatatatgt
     5341 attgaacgtt atgtttgtca agactaatga aatcgtatgg tgcaatccag taaaggccta
     5401 tggtcagctt actgttgcct atgccttttt tgctcatggc aagatcaaaa tatcgcccaa
     5461 cagtttctcg tttgtcacct gttggatttt ctacatattg caatgcatat tcaaacaatt
     5521 cccacaactc atttatatca ttgtcgcctc tgtcaccaag gaagtaatag aaagtggcgt
     5581 tttgcgggtt tacaacagga attcctgaaa agtccgttgg gatcgggctt tttaatccaa
     5641 agatgtcagc aatagcagct attactcttt tacgtgagtt ttctccaaga cctttgttaa
     5701 atagaccaat tactgtaaaa ggatcaatat ctactatctg attgcccctc tccaatgttg
     5761 gcatagcaat tcctgtaacc tcataaatac gccgtacctt ctcaatcaat t
//
LOCUS       DAONVY010000077         5807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10232_length_5807_cov_3.676460, whole genome shotgun sequence.
ACCESSION   DAONVY010000077 DAONVY010000000
VERSION     DAONVY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_5807_cov_3.676460"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PLI11_03230"
     CDS             <1..421
                     /locus_tag="PLI11_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51907.1"
                     /translation="LQIKVQSLALDMLYEALKDSASDINYPDNTEYMMPAIEYINRNI
                     ARSISVSELAAVMNMSLSTFRRSFTKTFGKSPMFFIIQRKLEIACEKLQYSDMSVSEI
                     AYLCGYSDPFYFSRLFKQKLGICPVNYRVMARTSTKL"
     gene            581..1300
                     /locus_tag="PLI11_03235"
     CDS             581..1300
                     /locus_tag="PLI11_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPZ51908.1"
                     /translation="MNEPIVVIVRSIIAFFTLLIFTRVLGKQQISQLTFFDYILGITI
                     GSVAASLTTDLDSRAWPHWVGLFMWTLLVWLIQWITTKSRKLSIYLDGEPTIVIMNGK
                     IMEDAMKKLRYRIDDLVEQLRQKDVYDITQVEFAIIEKDGRLSVLKKAEHQSVTRKDM
                     NVAVQPESVATEIIYDGVVIYSNLRQLKLDTKWLYGELKKMNIDSPGEVMYMSRKQDG
                     TLYVDLYKDHINRDNVTDITD"
     gene            1312..1668
                     /locus_tag="PLI11_03240"
     CDS             1312..1668
                     /locus_tag="PLI11_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HPZ51909.1"
                     /translation="MKIVKALIPIALIAVFVFVMNTAFIFHPFIKSIPELFDDIENSA
                     LNGDWDKASEKTDALYKELAQKKFRWMQFAIERKEMNNLIINLYKLKGCIKIEDVSGS
                     VDALYEMRANWSHLGS"
     gene            1765..4428
                     /locus_tag="PLI11_03245"
     CDS             1765..4428
                     /locus_tag="PLI11_03245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPZ51910.1"
                     /translation="MKKVLSIRGIIVLIFVIAMIVAVFIALTVVFVNWSLSARNTMKS
                     QAYDLHHTICDKIEQVLDISIMTIEVNSRHFENQLIDIQDEDALYYFFVSNLKSLDKN
                     IYSFSIGTPDGEYYGARRNENDVIEIMRNNKETNGESWYYSINDDLTIGRLTGKFGKF
                     DVRTRDWYQKAQTTRSVVFSDLYYHFVRPAFTVSVSKSILTKNGDFLGVLGVHIVLSD
                     LDNFLTETVKGKKGYAFIAERNTGLLVANSLGLKNYVEMDGERIRRNIYDIGNNVFDE
                     AYDKFNKTGISQFKYKGMYFNFVHSESGMDWIVCTAVPESYLMKDVRINIALTSVLVI
                     LFLISSIIIYYGITNKLVRPVNHLIESTEKFARGDLSTRVDVSRNDELGKITRAFNEM
                     AETIDSLVNDLEKKVKERTSALEDLNIQIKEKEEQLRIILDSAAECIFGVDVNGVCVF
                     CNKSCIQVMGYDSEEELLGQDVHELVHGYDMQGNKVKRSDCKVVKSIIDGIGYFSDDE
                     TFVRKDGTQINVSYYSYPQFKDGKIIGAVVTFVDITSRKEEERRIMYLSNHDSLTGFV
                     NKRYFEQLLDEYDNRENLPLAIIYADLNGLKLTNDIFGHDAGDELIVKAANCIRKNVK
                     EDSVTARVGGDEFVVLMPNTDEDAVKEIIDSINKDVSDEYVHAVRCTIAMGYSIKNSR
                     FQDIRLILTNAESEMYKNKGRMSGKFSREAISSFMKLLHERAEREKIHSRRVSLLCEE
                     MAKALGFERPDVRKLKEIGYLHDIGKIGVDKNLLTKSTLTEDEMQTMQQHSFIGYRIL
                     NLFEYTVDYADSVLSHHEKWDGSGYPKGLKGEEIPIISRIVAIAEAFDRMLDKMSFEE
                     IIREIEFESGRYFDPGLVPVFLEVVKKTNFK"
     gene            4562..4852
                     /locus_tag="PLI11_03250"
     CDS             4562..4852
                     /locus_tag="PLI11_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51911.1"
                     /translation="MDDTCNKYGEGTGTVRIRDYGGYRVICFDRVRVLELNDEKFIVN
                     NVPQFWISGVHIKQQGITFTPAFMKRWNRGGNYIKDLKNVLPTSEQLKRNKL"
     gene            4968..5657
                     /locus_tag="PLI11_03255"
     CDS             4968..5657
                     /locus_tag="PLI11_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51912.1"
                     /translation="MKNGFIIFLLSILLVANVNEYLKQYSYTLVFEETFQTEDGSLKD
                     EKDLIKDWVMDDLNISKITSQGRFELSGEFYSEKEFCQYKLEFHMAPKDAEVFGVVTR
                     GGDVSVTFVIGDYDKFTVSVKGHEQSAGFPEGFDLSILNKYSIYDDGSTVSIFVNDTH
                     LYSIDLKERYATEKGSVGFYSENGLMTVDNINVYTMNPRNPKTRDDFLVICLIAFVFL
                     SYFFVKIRIDS"
     gene            complement(5683..>5807)
                     /locus_tag="PLI11_03260"
     CDS             complement(5683..>5807)
                     /locus_tag="PLI11_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPZ51913.1"
                     /translation="KPGMLQTAGRFMTIPKGAKLKKIDLETIKKKFIDLGYVVK"
BASE COUNT         1891 a          852 c         1348 g         1716 t
ORIGIN      
        1 attgcaaata aaggtgcaga gtcttgcgct tgatatgctg tatgaagcac taaaagacag
       61 tgcaagtgat ataaattatc ctgataatac tgaatacatg atgcctgcaa ttgagtatat
      121 aaataggaat attgcaagga gcattagtgt aagtgaactt gcagctgtaa tgaatatgtc
      181 gttatccacc ttcaggcgca gttttactaa aacctttgga aaaagtccta tgtttttcat
      241 tattcaaagg aaactcgaga ttgcttgtga aaaactacag tattctgaca tgtcggtaag
      301 tgagatagca tatttgtgcg gatattctga tcctttctat ttttcaaggc ttttcaagca
      361 gaaacttggc atatgtccgg tgaattatag ggtaatggca agaacttcta caaagttgta
      421 aagtaacaaa ttgtgctggc atttgaaaag agtgtgcaat atgatgcgaa aatggagaag
      481 aggggaatta tcaagtgtat aattcaaaag acataattgg ttgattaagg catgtttttt
      541 cctctggttg gagataatag cttaaaaagg gggaagaagc atgaatgaac cgattgtagt
      601 tattgtcagg agtattattg cctttttcac acttctgatt tttacaagag ttcttggcaa
      661 gcagcagatt agtcagctta ccttctttga ttacattctt gggataacga ttggttctgt
      721 ggctgcatct ttgaccaccg atctggattc aagagcatgg cctcattggg ttggtctttt
      781 tatgtggacc ttgctggttt ggttaatcca gtggatcacc actaaatcaa gaaagctttc
      841 catatatctt gatggtgagc ctacgatagt tatcatgaac ggaaagataa tggaagatgc
      901 catgaagaaa ctaagataca ggattgatga tttggttgag cagcttaggc agaaggacgt
      961 atatgacata acccaggtgg agtttgcgat aattgagaag gatggaaggc tttccgttct
     1021 caaaaaggct gagcatcaaa gtgttacacg aaaggacatg aatgtggcgg tgcagcctga
     1081 gagcgtggca acagaaataa tttatgatgg tgttgttata tactcaaatt taaggcagct
     1141 gaaactggac acaaagtggc tttatggaga gcttaaaaag atgaatatag attcaccggg
     1201 tgaggtcatg tacatgtcaa gaaaacagga tggtactttg tacgttgatt tgtacaaaga
     1261 tcatattaac agggataatg ttactgacat aaccgactaa ggaggtttaa tatgaagata
     1321 gtaaaggctt taattcctat tgctttgatt gctgtgtttg tttttgttat gaatacggcc
     1381 tttatctttc atccgtttat taaaagcata cctgaacttt ttgacgacat agaaaacagc
     1441 gctcttaatg gtgactggga caaggcatcc gagaagacgg atgctcttta caaagaattg
     1501 gcacaaaaaa agtttcgttg gatgcagttt gccatagaaa gaaaagaaat gaacaacttg
     1561 ataataaatc tgtacaagct aaagggatgc attaagattg aagatgtatc agggagtgtc
     1621 gatgcacttt atgagatgag ggctaattgg tctcatctgg gaagctaaaa aaataattgt
     1681 aaatattatt aaaataaggt acaatgggtt gaatgtttta aaatataaaa ttttattgct
     1741 tgaatttgtt gcagggtgtc atctatgaag aaggttctgt ctattagagg tataattgta
     1801 ctaatattcg taatagccat gatagtagca gtgtttattg cgcttacggt ggtgtttgtt
     1861 aactggagcc tttcagcccg gaacactatg aagagtcagg cctatgacct gcatcacacc
     1921 atctgcgaca agattgagca agttctcgat atatccataa tgaccatcga agtgaacagc
     1981 agacattttg aaaaccagct aatcgatatt caggacgaag atgcgctata ttattttttt
     2041 gttagcaacc tgaagtctct tgacaaaaat atatacagtt tcagtatcgg cacaccggat
     2101 ggagagtatt acggggctag aagaaatgag aatgacgtta ttgagataat gagaaacaat
     2161 aaggaaacca atggtgaatc atggtactat tctataaatg atgatctgac aattggccgg
     2221 ctaacaggca agtttggaaa gtttgatgta agaacgaggg attggtacca aaaggcccag
     2281 acaacacgca gcgttgtttt ttcagacctc tattatcatt ttgtaaggcc tgcatttaca
     2341 gtttctgttt caaagtccat tttaacaaaa aacggagatt ttctgggtgt gttgggcgtc
     2401 cacattgttc tttcagacct tgataatttt ttgacggaaa cggtcaaagg caagaaggga
     2461 tatgctttca tagctgaaag gaacacggga ttgcttgtag caaactctct tggtcttaaa
     2521 aattacgttg aaatggacgg agagaggata agaagaaaca tttacgacat tggaaataat
     2581 gtttttgatg aggcatacga caagttcaat aaaacaggca taagccagtt taagtacaaa
     2641 ggcatgtatt tcaatttcgt tcacagtgaa agcggtatgg attggattgt gtgtactgct
     2701 gttcccgaaa gctatctaat gaaggacgta agaatcaaca ttgcacttac atcggttttg
     2761 gtaattcttt ttctcatatc atcaattatc atatactacg gtataaccaa caaattggta
     2821 agaccggtaa accatcttat agaatccact gagaaatttg caagaggaga cttgtcaaca
     2881 agagttgatg tttcaagaaa cgacgagctt ggaaagatta caagagcatt caatgaaatg
     2941 gcagaaacca tcgattctct tgtaaatgat ttggagaaaa aggtcaagga gaggacttct
     3001 gcactggagg atttgaatat ccaaattaaa gagaaggaag agcagcttag aattattctt
     3061 gattccgcag cggaatgtat tttcggtgtt gatgtaaacg gagtttgtgt gttctgcaat
     3121 aaaagctgta tacaggtgat gggatatgac agcgaggaag agcttttggg gcaggacgtg
     3181 catgagcttg tgcacggtta tgacatgcag ggaaacaagg taaagagaag cgattgcaag
     3241 gttgtcaaaa gcataattga cggtattgga tatttctctg atgatgaaac ctttgttagg
     3301 aaggatggta ctcaaatcaa tgtctcatat tactcgtatc cccagttcaa ggatggcaaa
     3361 ataattggtg cagtggttac ttttgtagat attacaagta ggaaggaaga agagaggaga
     3421 atcatgtatc tttcgaacca cgactccctt acgggttttg tcaacaagag atattttgaa
     3481 caactgttgg atgaatacga taatagagag aacttgcccc ttgctataat ttatgcggat
     3541 ttgaatggtc ttaaacttac aaacgatata tttggacatg atgcgggcga tgaacttatt
     3601 gtaaaggcgg ctaattgcat aaggaagaat gtaaaggaag acagtgtgac tgcaagggtt
     3661 ggaggagacg agtttgtcgt tcttatgccg aatacggatg aagatgctgt aaaagagatt
     3721 atagattcaa taaacaagga tgtttctgat gaatacgtgc atgctgttcg atgtactatt
     3781 gcgatgggtt acagcatcaa gaacagcagg tttcaggata ttagacttat acttacgaat
     3841 gccgagagtg aaatgtacaa gaacaaggga agaatgagtg gcaagttcag cagagaagcc
     3901 ataagttctt ttatgaaact tttgcatgag agagctgaaa gggagaagat acattccagg
     3961 agggtaagtc tgctttgcga agagatggca aaagctcttg gttttgagcg tcctgatgtc
     4021 agaaaactca aagagatagg atatttgcat gacattggaa agattggagt tgacaagaat
     4081 ctccttacaa aatcaactct aacagaagac gaaatgcaaa ccatgcagca gcactcgttt
     4141 attggttata gaatcctcaa tttgtttgag tatacggttg attatgcaga cagtgtactt
     4201 agtcaccacg agaagtggga cggttcaggc tatcctaaag gtttgaaggg cgaagaaata
     4261 cccatcatat caaggatagt tgcaattgca gaggcttttg acaggatgct ggacaaaatg
     4321 tcctttgagg aaattattag agaaattgaa ttcgagtctg gcagatattt tgatccgggc
     4381 cttgttccag tgtttttgga ggttgtaaag aaaacaaatt ttaaatgaaa tgtaaacagt
     4441 ataaatttat cataaacaac atggtaaaca acaagatgtt gtggtataat ccataatcgt
     4501 gtggtaaaca tatcctgtta aaacagaatg cgcattattt atggagagga agatattgac
     4561 gatggatgat acctgcaaca agtatggcga aggtactggc acggtacgta ttagggatta
     4621 tggcgggtac agggtaatat gttttgacag agtacgagtt ttagaactga acgatgaaaa
     4681 atttatagta aataacgtac cacaattttg gatttccgga gtacatatca aacaacaagg
     4741 tattacattt acacctgcct ttatgaaacg ttggaacaga ggtggcaatt atatcaagga
     4801 tttgaaaaat gtattgccaa caagcgaaca actaaaacgt aacaagcttt aatcacatgt
     4861 gaaagccccg ttgtaaacgg ggtttttgct tgtgaaaaaa taacaaaata tttttcagtc
     4921 aaaccgtcca tatttagtat agttatacaa tggaaaagga gattggcgtg aaaaacggtt
     4981 ttatcatatt tcttcttagc atactgctgg ttgccaatgt aaatgaatat ttaaaacaat
     5041 actcctatac gcttgtgttt gaagagactt tccagactga agacggctcg ttaaaagatg
     5101 aaaaggattt gataaaggac tgggtgatgg atgatttaaa tatatcaaaa ataacaagcc
     5161 agggcaggtt tgagttgtcg ggagagtttt attcagaaaa agaattttgt caatacaagc
     5221 tggagtttca tatggcacca aaagacgcag aagtctttgg tgttgtgaca aggggtggag
     5281 atgttagtgt aacttttgtg attggggatt atgacaagtt tacagtgagt gtaaaaggtc
     5341 atgaacaaag tgcaggtttt cccgaaggtt ttgatttatc catattaaac aagtattcca
     5401 tttatgacga tgggtctact gtctccatat ttgtaaatga tactcattta tacagtattg
     5461 atttgaaaga acggtatgcg acagaaaagg ggagtgtggg tttttacagc gaaaacggat
     5521 tgatgactgt tgataatata aatgtgtata cgatgaatcc caggaatccc aagactaggg
     5581 atgactttct tgtcatatgt ctgattgcgt ttgtttttct ttcgtatttc tttgtaaaga
     5641 taaggataga ttcataaaat aattcacccc cgaaggggcg aattatttta ctacatatcc
     5701 caaatcaata aatttctttt tgatggtctc caagtctatt ttcttaagct ttgctccttt
     5761 tggtattgtc atgaacctgc cggcggtttg aagcattccc ggttttg
//
LOCUS       DAONVY010000078         5796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10251_length_5796_cov_3.444870, whole genome shotgun sequence.
ACCESSION   DAONVY010000078 DAONVY010000000
VERSION     DAONVY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10251_length_5796_cov_3.444870"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1567)
                     /locus_tag="PLI11_03265"
     CDS             complement(200..1567)
                     /locus_tag="PLI11_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51914.1"
                     /translation="MNKNSCLLSLKNSNVVPIDWSNLNTKPLTGEMTEGIINIINNGN
                     KFIVNDWWNKVWNFNDDNHKEYLTMKGNKEHSIRPLTHMSLALSASIALNIYDENTTG
                     VSVKDAKDMAVKLIKSVAKAHYSNTPEGWGNAWQSAYWAADVALASWFFWDNFSKEDQ
                     KYIQNCIVTEANRFIDYIVPYYMDRKGNILFPGDSKAEENAWNSNILSAACAMMPNHE
                     NYNLWMYKNMELILSAFARPSDTLRDIEINGFRLNEFLKGSNINEDGTVVNHNIIHPD
                     YQATMAVKLLNGVYFAFGKQKVPQAVVFNGDVIYESFVNLDLKDLNKDMAGQYIYKRK
                     HDGSASHEINYPNGTDWGTDRQICFFLMDVYADMLKLDKNLPLKAKDFALARLPVMKA
                     MQSRTENGQYYLSTDSDRYRSREEWVMWHAIHSYMALWVGENGMYNITNESPFKPDMP
                     SQDTP"
     gene            1832..2599
                     /locus_tag="PLI11_03270"
     CDS             1832..2599
                     /locus_tag="PLI11_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPZ51915.1"
                     /translation="MAGLFDLTNKVAVVTGASSGLGVQFAKALARQGANVALVARRVE
                     RLNDVKKEIEALGVKSLAVKCDVSNVEEIKAAVKEIVDYFGTIDILVNNAGVGAAEPA
                     ETQSDDTWEWVMNVNVNGVYYFAREVGKVMIEKKYGKIINVGSIHSTVAMPGLPISAY
                     CTSKGGVEMMTKALANEWAKYNITVNAIGPAYFPSEMTDDVLNDEGFAQVVKTYCPMG
                     RPGRDGELDGAIVYFASDASSYTTGQLLSIDGGWTAI"
     assembly_gap    2810..2819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2820..4906)
                     /locus_tag="PLI11_03275"
     CDS             complement(<2820..4906)
                     /locus_tag="PLI11_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPZ51916.1"
                     /translation="MIRIIIRLTSLFLLSACLASFIGCGKITSPIDKRSIALIDNNVK
                     YDPEVIPIDWSKFDIKPLTEELADGTIYLIRNGTKYIVNDWWNRVWRFSLQDSKEYLA
                     MTGRNEHDIRPLTHMALTLSSSIALGIYDESVTGVSKENAKNMAMKLTKSVAKAHYSN
                     TEGGWGNAWQSALWAFDVALAGWLFWEEFSEKDREYIQNCVVTEANRFIEYTVPYYRD
                     KSGKIRFPGDSKAEENAWNSNILSIACAMMPNHENYNAWMYKNVELMLSAFARPSDAD
                     SSKTVSGFVLKYLLEGSNVNEDGTVINHNIVHPDYQATVALNLLNGISFALANTKIPE
                     AVVFNGDVIYETFVNLDLKDINNSMAGQYIYKRKNDGSASHEINYPNGTDWGTDRQIC
                     FFLVDVFADALNLDKNCKIKAKDFALARLPVMKAMQDRNDNGQYYTASDSDKYKSREE
                     WVMWHATLSYMALWVKENNLFVFDKELPLKPAPLNEVKLEIPHVLQSGTTSKATYSLN
                     PGLVILPDDLKIAYSSSDKDIVSITNTGTVYVKKEGKVTISITVTYKDIVVSDSVEIE
                     VVKDKKPIYDFEDLPLGNTLPLDFRWNNVGNAEVVQEDNGNKALRLSGAGYNYIFIGD
                     DFEEDFTVTFRLKMESHQQKGGAHLLFREDGLLFTGRNGYQFCIGDTMNGEYGLELRK
                     RDTSLSDNKYAAC"
     gene            5105..>5796
                     /locus_tag="PLI11_03280"
     CDS             5105..>5796
                     /locus_tag="PLI11_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51917.1"
                     /translation="MKKGLSDVKTSFKYDGNQYFGVDDFDLISEKGSIVDNVVYEEYV
                     FRFLDNIDIVVQTEYNKEFNAFGYTVWFENKGDANTKVFSELFCADMKIQGGNPVLKG
                     ILGDHIHQYKPYKINLKEEEASFKQETGRATHVVFPYFLLETDNGSNFMALGWAGTWS
                     ASFRYEDEGARFIAKGVNNLCTYLKPGEKIRTPLVLTMSSDIVRDTERAMNLWRRWFV
                     KYNMPPVKPMIT"
BASE COUNT         1591 a         1136 c         1206 g         1853 t
ORIGIN      
        1 gaagttggaa agtacgttag aacctgctgg ctttagccac gcagagtttc aggagcttgg
       61 tgagattgaa gtgttcaaca aataactaca aattattaaa atatcgtgta gagctgcagg
      121 ttaaagcgat gggagaaatt ttcccatcgc tttaaccaac tgtaggtata atatacacag
      181 gaggtgtagt taagctaagc taaggtgtat cctgtgatgg catatctggt ttgaaaggac
      241 tttcgttggt aatgttatac atgccgtttt ctcctaccca taaagccatg taggagtgaa
      301 ttgcatgcca cataacccat tcctcccggc ttctgtatct gtcgctatct gtagacaggt
      361 agtactgtcc attttccgtt ctgctttgca tggctttcat tacaggcagt cttgcaagcg
      421 caaaatcttt tgcttttaat ggcaagtttt tatccagctt cagcatatct gcatacacat
      481 ccataaggaa gaaacatatt tgtctgtcgg ttccccagtc ggtaccattt gggtaattta
      541 tttcgtggga agcgctcccg tcatgctttc ttttgtaaat atactgtcct gccatgtcct
      601 tgttcaggtc tttcaaatca aggttgacaa agctttcgta tataacatct ccgttgaaaa
      661 cgactgcttg cggtactttc tgctttccga aggcaaagta gacaccattt agaagtttta
      721 cggccatggt tgcctggtaa tccgggtgaa tgatgttgtg gttgaccacg gtgccatctt
      781 cattgatgtt ggaaccttta agaaattcat taagcctaaa gccgtttatt tcgatatccc
      841 ttaacgtatc gcttggtctt gcaaaggccg aaagtataag ctccatgttc ttatacatcc
      901 ataagttata gttttcgtga ttgggcatca tggcgcaagc tgcagaaagt atgttggaat
      961 tccaggcgtt ttcttccgct tttgagtcgc ctggaaacag tatatttcct tttctgtcca
     1021 tataataggg gacaatgtaa tcaataaatc tattggcttc ggttactatg cagttttgaa
     1081 tgtatttttg atcttctttg ctaaaattat cccaaaaaaa ccaactagca agagctacat
     1141 ctgctgccca atacgcactt tgccatgcat ttccccaacc ttcaggagtg tttgagtaat
     1201 gggcttttgc gacggatttt atcagcttta cagccatatc tttagcatct tttactgata
     1261 ctccggttgt attttcatca tatatgttta gtgcaatgct tgcggataat gccagtgaca
     1321 tatgggttag tggtcttatg ctatgttctt tgttaccttt cattgtcagg tattctttat
     1381 ggttatcgtc attaaaattc cagaccttgt tccaccagtc attcacaata aatttatttc
     1441 cattgttaat aatatttatt attccttctg tcatctcgcc ggttaaaggt ttggtgttca
     1501 agttgctcca gtcaatagga actacatttg aattttttaa tgataacaga catgaatttt
     1561 tgttcattgt ttcacctcat aagctgtttc gaatatttta cactatatta agtggttact
     1621 cttccaattt taaaccatat ttaataattt gggtgtaata atcggaactc tttttgaatt
     1681 cataaaaact tcactaaaaa tatttgcact ggatatatta ttttgttaaa atgttgtcaa
     1741 agtcatttcc tataacaatt gtaggagatg gcataaatta gggagttagg acaaattata
     1801 taagtaatat ttatgaaaag gagagtataa tatggcagga ttatttgatt tgacaaacaa
     1861 ggttgcagtc gttacaggag cttcttcggg tctgggagtc cagtttgcaa aagcattggc
     1921 aagacagggc gcaaatgttg ccttggttgc aaggagagtt gaaaggctga acgatgtgaa
     1981 gaaggagata gaagcattgg gagtaaaaag cctggcggta aaatgcgatg tctccaatgt
     2041 agaggaaatc aaggctgcgg ttaaagagat agttgattac tttggaacaa tagatattct
     2101 tgtgaacaat gctggtgttg gcgctgcgga acctgcggaa acccagtccg atgatacttg
     2161 ggagtgggta atgaatgtca atgtaaacgg cgtatattat tttgcccgcg aggttggcaa
     2221 ggttatgatt gagaagaagt atggaaagat tataaatgtt ggatcgatac acagcactgt
     2281 agccatgccg gggctgccaa tcagtgcata ctgcacctca aagggtggag ttgaaatgat
     2341 gacaaaggct cttgcaaatg agtgggcaaa gtacaacatc acggttaatg cgataggacc
     2401 tgcatacttc cccagtgaaa tgacggatga tgttcttaat gatgaaggct ttgcacaggt
     2461 tgtaaaaacg tattgtccca tgggaaggcc aggaagagat ggagaacttg atggtgcaat
     2521 tgtttacttt gcttccgatg catcaagcta cacaacaggt cagcttctgt ctatagatgg
     2581 aggatggact gcaatatgac ataaaaaagg cggtgggttt cccaccgctt tattaatgca
     2641 gagttatcaa tatatcgtca aacagtactg tatccagata agatgcaatg gcaatgcagc
     2701 ctttatcaaa tcctgtcaga gtatgggtca tggcttcctc tccatccatt tttaatgtta
     2761 ccttgtttcc aaccatgctt atctcaaagt cgtgccactc atagtcaagn nnnnnnnnnt
     2821 cgcaggcggc atacttgttg tcggaaagac tggtatcgcg ttttctaagc tcaagtccgt
     2881 attctccatt cattgtgtcg cctatgcaga actggtatcc attacgtcca gtaaacaaaa
     2941 ggccatcttc ccggaaaaga agatgcgccc ctcctttttg ctggtgggat tccatcttca
     3001 atctaaaagt tacagtaaag tcttcttcaa aatcatcacc tatgaaaatg tagttgtatc
     3061 cagcacctga aagtcttagt gctttgtttc cgttatcttc ctgaacaact tccgcattgc
     3121 ccacattgtt ccacctaaag tccaaaggaa gggtgttgcc aagcggcagg tcctcaaagt
     3181 cgtaaatggg tttcttgtct ttgaccacct ctatttcgac gctgtctgaa acaactatgt
     3241 ccttgtatgt gacagttatt gaaattgtga cctttccttc cttttttaca tatacagtac
     3301 cagtattcgt tatggatact atatccttgt cgctggatga gtatgctatt ttcaaatcat
     3361 caggaagaat aaccaatcct ggattcagtg aataagttgc cttgcttgtg gtgccagatt
     3421 gcaggacatg cggtatctca agcttaactt cattaagcgg tgcgggtttt aagggcagtt
     3481 ccttgtcaaa aacaaacaga ttgttttctt ttacccacaa tgccatgtag gacaaggtag
     3541 catgccacat gacccattcc tcacgactct tgtacttgtc gctgtctgag gcggtgtagt
     3601 attggccgtt gtcatttctg tcctgcattg ccttcataac aggaagtctt gccagtgcaa
     3661 agtctttggc cttgatctta cagttcttgt caagatttag tgcatcggca aagacatcca
     3721 ccaggaagaa acatatctgc ctgtctgttc cccagtctgt tccgttcgga tagtttattt
     3781 catgggatgc gcttccgtca ttcttccttt tgtatatgta ctgacctgcc atgctattgt
     3841 ttatatcttt aaggtcaagg tttacaaagg tctcatagat cacatctcca ttgaagacaa
     3901 cagcttcagg tatttttgta tttgccagcg caaagctaat cccgttcaaa agattgagag
     3961 ctaccgtggc ctggtaatcc ggatgcacta tgttgtggtt gattacagtt ccatcttcat
     4021 ttacatttga accttccagc aggtacttaa gaacaaaacc gctcacggtt tttgatgagt
     4081 ctgcgtcact tggccttgcg aaggctgaaa gcataagttc cacattcttg tacatccagg
     4141 cgttgtagtt ttcatggttt ggcatcatgg cgcaggcaat tgacaaaata tttgaattcc
     4201 atgcgttttc ttcagccttt gagtcgccag gaaatcttat tttcccactt ttatccctgt
     4261 agtagggcac tgtatattca ataaatctgt ttgcttccgt tactacgcag ttttgaatat
     4321 attcacgatc cttttcgcta aattcctccc aaaacagcca acctgccagg gcaacatcga
     4381 atgcccataa agcactctgc caggcattgc cccaaccgcc ctctgtattt gagtagtgag
     4441 cttttgcaac ggattttgta agtttcatcg ccatgttttt ggcattttcc tttgaaactc
     4501 ctgtcacaga ttcatcatat attcctaagg caatgctgga agacaaggta agcgccatat
     4561 gcgtcaaagg ccttatgtca tgttcgtttc tgccggtcat tgcaagatat tcttttgaat
     4621 cctgcaggct gaatctccaa actctgttcc accagtcatt aactatgtac ttggttccat
     4681 ttctgattag atatattgtt ccatccgcca gctcttctgt caatggttta atatcaaact
     4741 tgctccaatc tattggtatc acctctggat catactttac attgttgtca attaatgcaa
     4801 tactcctttt atctatcggg cttgttatct tcccgcaccc aatgaaggaa gcaagacaag
     4861 cggacaaaag aaacaggctg gtaagtctaa ttattattct tatcatggtt tccctccata
     4921 attctggtta tcaaaatcag ttttcagtgc aacttaatcc attgtgttta ttttatcaca
     4981 gtatttacat tatacatacc cctcttttta tttcattata aattttggaa aggacataat
     5041 attcgttata cttttcggaa catgtatggt acaattactg caacgaaatg taggaggaag
     5101 aaaaatgaaa aagggacttt cggatgtcaa gacaagcttt aaatatgatg gcaatcaata
     5161 ttttggagtt gatgactttg atttgatttc ggaaaaagga agtattgttg acaatgttgt
     5221 atatgaagag tatgtattta ggtttttgga caacatcgat attgttgtgc aaacagaata
     5281 caacaaggaa ttcaatgctt ttggctacac tgtctggttt gagaacaagg gtgatgcaaa
     5341 tacaaaagtg ttctccgaac ttttctgtgc tgacatgaaa atacagggtg gaaatcctgt
     5401 attgaaagga atactgggag accatataca ccagtacaaa ccatataaaa tcaatcttaa
     5461 agaggaagag gcaagtttca aacaggaaac ggggagagcc acccatgttg tctttccata
     5521 ctttttgctg gagacggata atggttcgaa cttcatggct cttggctggg caggcacctg
     5581 gtcggcaagt tttcgatatg aagacgaagg agcaagattc attgccaagg gagttaacaa
     5641 cctttgtaca tatctaaaac caggtgagaa gataaggact cctcttgttt tgacaatgtc
     5701 gtcggatatc gttagagata cggaaagggc aatgaatttg tggcgcagat ggtttgtaaa
     5761 atacaatatg ccgcctgtaa agcctatgat tacgaa
//
LOCUS       DAONVY010000079         5784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10274_length_5784_cov_3.592774, whole genome shotgun sequence.
ACCESSION   DAONVY010000079 DAONVY010000000
VERSION     DAONVY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10274_length_5784_cov_3.592774"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLI11_03285"
     CDS             complement(<1..401)
                     /locus_tag="PLI11_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPZ51918.1"
                     /translation="MSSRFGNFGGQYVPETVMNALCELETSLSDALKDEAFLKEYKYY
                     LKEYVGRPTPLYFAENLTKELKGAKVYLKREDLNHTGAHKINNVIGQVLLARRMGKKR
                     IIAETGAGQHGVATATVAAMFGMECEIFMGE"
     gene            complement(438..1010)
                     /locus_tag="PLI11_03290"
     CDS             complement(438..1010)
                     /locus_tag="PLI11_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPZ51919.1"
                     /translation="MVRIKVCGLKRDVDISYVNELKVDYAGFVFAKSRRQVSLEEAKK
                     LVDKLDSGIKRVGVFVNERAYEIQKAVEVLSLDIVQLHGEEDLGEYLGLDVDIWKAVR
                     VSCKEDVERARLYKCPGVLFDAFVEGMYGGTGKVFDFGLIEKEELNSKVILAGGLNCN
                     NVSLAIERVKPYAVDVSSGVETEGVKDLIR"
     gene            complement(1011..1784)
                     /gene="trpC"
                     /locus_tag="PLI11_03295"
     CDS             complement(1011..1784)
                     /gene="trpC"
                     /locus_tag="PLI11_03295"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPZ51920.1"
                     /translation="MMLERIVFEKKKRLAIEKEQYSLSDLEKKIKDTKRKDVFKKALS
                     GKIVRIFAEIKKASPSKGIINDKLDHVQVARMYEEGGADAISVLTEEKFFLGSDRILE
                     NVREVVNCPVLRKDFVVDEYQLFQTKALGADAVLLIVALLKERTKEFYTLAKDLGLEC
                     LVEVHDEREVYTALEAGARVIGINNRDLKDFSVSLETTKRLVPLIPDDVVKVSESGIK
                     TYEDISYLRSFNVNAFLIGETLSKSKNIYEDIKSLKGGK"
     gene            complement(1781..2782)
                     /gene="trpD"
                     /locus_tag="PLI11_03300"
     CDS             complement(1781..2782)
                     /gene="trpD"
                     /locus_tag="PLI11_03300"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPZ51921.1"
                     /translation="MLDAMLKKIIMREDLSYSEAVDSMNAIMKGEATTSQVGAFLAAL
                     RMKGETVNEITGCAVAMRNSALKVEIEDYAIDTCGTGGDGGRTFNISTASAIIASCAG
                     IKVAKHGNRAISSKSGSADVLNALGIKIQMTPEVFVESVNKANFGFLFAPYYHTSMKN
                     VALVRRELGIRTVFNILGPVTNPAGVKGQVLGVFDKGLVSMLAEVLRNLGTKRAMVVH
                     GLDGLDEVTTTTKTLVSEIKDGEITSYEIDPADFNIPYARLEDIKGGDAEYNAKIITD
                     IFKGKKGFERDIVLLNAACALYVGNKVESINEGLVMAEYLVNSGAAMAKLEELREVV"
     gene            complement(2775..3347)
                     /locus_tag="PLI11_03305"
     CDS             complement(2775..3347)
                     /locus_tag="PLI11_03305"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPZ51922.1"
                     /translation="MILVIDNGDSFAYNIYQCVGEVFPNVRVVSNLVAISEIQDMNVK
                     GIVMSSGPGRAKDAGNCVGIVKELSSQIPILGIGLGMEVIGCAYGGGVLPDVKGMHGK
                     TSIVLHDGKGIFKGVKNPILTARYHSFKLNESSLPDVLEVCAHTLEGTIMAVRHKSLP
                     VYGLEFHPESIITEKGKKIIGNFVGGIKNA"
     gene            complement(3344..4771)
                     /gene="trpE"
                     /locus_tag="PLI11_03310"
     CDS             complement(3344..4771)
                     /gene="trpE"
                     /locus_tag="PLI11_03310"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPZ51923.1"
                     /translation="MIYPTKEEFLDLKRNSASYIPVKLEMNADLMTPIGLFYSLKGEK
                     KFLLESAECGKSWGRYSLLGFTPYLTIKSYGEEVWVGDRKFIGSVLDVLKEVMGRYEV
                     SETKHGLPFFGGAVGYVGYDVARQYLNIKSNNKDDLNLPDAYLMLYKEVLVYDHFTHR
                     LYIIHNVLPGDDRSYDEIVKKLLDIKEKIQSSNACITESAYFQTKGFESNFDEQEFVE
                     VVDKAKKYIELGDVFQVVLSQRFIQETNIEPFDAYRKLRTLNPSPYMFHIDFGDFQVV
                     GSSPESLVKVKDGVVATNPIAGTRKRGRNEEEDNLLREELLNDAKERAEHVMLVDLGR
                     NDLGKVCKFGSVKVDKYMEIEMYSHVMHIVSTISGQLQEGKDCFDALYACLPAGTVSG
                     APKPRAMEIIDGLENVSRGIYAGSIGYFDFSGNMDMCIAIRTIVFKDRKACIQAGCGI
                     VYDSCPEREYEEIQNKAKALKEVLG"
     misc_binding    complement(4821..5138)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5212..>5784)
                     /locus_tag="PLI11_03315"
     CDS             complement(5212..>5784)
                     /locus_tag="PLI11_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ51924.1"
                     /translation="NEDMANCLLDRITEIKCTFARMYAKAGCDILRLGDDVSTQLDMM
                     MSPDMWKKYLKGRLEKVIACAKEINPDIFIFYHGDGNLTKIIPDLIEIGVEILNPVQP
                     ECMDPLRIKDEYKDKLTLWGTLGTQTLMPFGTPSEVRDFCMKMIDKAREDNGGLVLAP
                     THVLEPEVPFENILAFVETVKVYNGEKIRI"
BASE COUNT         1568 a         1543 c          889 g         1784 t
ORIGIN      
        1 tcctctccca tgaaaatctc gcactccatg ccaaacatgg cagccactgt agcggttgca
       61 acgccatgct ggcctgctcc cgtttcggca ataattcttt tcttccccat gcgtcttgcc
      121 aggagaacct gcccaatcac attatttatc ttgtgggctc cggtgtgatt caagtcctct
      181 ctttttaaat atacctttgc accctttagt tccttggtaa ggttttctgc aaagtacaaa
      241 ggagttggac ggccaacata ctcctttaga taatatttgt attccttaag aaatgcctca
      301 tcctttaaag catcacttaa acttgtctca agctcgcaca gtgcattcat tacagtctca
      361 ggcacatact gccctccaaa attaccaaat ctagatgata ttttttccat attccctcac
      421 cctcttcaca aactctttca tcttatcaaa tcctttacac cctctgtttc cacaccgctg
      481 ctcacatcca ccgcatacgg ttttaccctt tcaattgcca aagatacatt attgcaattc
      541 aaacctcctg caagtattac ttttgagttt aattcttcct tctctatcaa accaaaatca
      601 aataccttcc cggtaccccc atacatgcct tccacaaacg catcaaaaag aactcctggg
      661 catttgtata accttgccct ttccacatct tccttgcatg aaaccctaac agccttccat
      721 atgtccacat caagaccaag atactcgcca aggtcttctt ctccgtgaag ctgcacaata
      781 tcaagggaaa gaacttctac agccttttga atttcataag ccctttcatt tacaaatacc
      841 cccacccttt ttatgccaga atcaagcttg tcaacaagct tttttgcttc ctcaagagac
      901 acctgccttc tactttttgc aaacacaaac cctgcataat ccactttaag ttcgttgaca
      961 taggaaatgt ctacatctct tttcaaccca cacaccttta tacgtaccac tcatttccct
     1021 ccctttaagc tttttatatc ctcataaata ttcttgcttt tggacaaggt ctctccaatc
     1081 aagaaagcat ttacattaaa agaacgaaga taacttatgt cttcatatgt tttaattccg
     1141 ctttcagaaa cctttaccac atcatccgga atcaaaggaa caagtctttt tgtggtttca
     1201 agacttacag aaaaatcttt cagatctcgg ttgttaatac caataactct agccccggct
     1261 tcaagagcag tataaacttc cctttcatcg tgcacctcca caaggcactc aagaccaaga
     1321 tcctttgcca gggtgtaaaa ctcttttgtt ctttccttga gaagggcaac tatcagcagt
     1381 acggcatcgg cgcctaatgc ttttgtctga aaaagctggt attcgtcaac aacaaagtct
     1441 tttcgaagaa cggggcaatt taccacttcc cttacgtttt ccaaaatcct gtcacttcca
     1501 agaaagaact tctcctcagt caacacggat attgcatcag caccaccttc ttcatacatc
     1561 cttgccactt gtacatgatc aagcttgtca ttgattatcc cttttgaagg agatgctttc
     1621 tttatctcgg caaatatcct tactattttg ccagacaagg cctttttaaa gacatctttt
     1681 cttttggtat cctttatctt tttttccaaa tcggatagac tgtattgctc cttttcaatt
     1741 gcaagccttt ttttcttctc aaaaactatt ctctcaagca tcaaaccacc tctctaagtt
     1801 cttcaagctt tgccattgcc gctcccgaat tcaccaggta ttcagccata accaaacctt
     1861 cattgatact ctctacctta tttcctacat acaaggcaca cgctgcatta agaagcacaa
     1921 tgtccctttc aaatcccttc ttccccttga atatgtcggt tattatcttc gcattgtact
     1981 ccgcatctcc acctttgata tcctcaagtc ttgcataggg aatattgaaa tccgcaggat
     2041 ctatttcata acttgttatt tctccatctt ttatctcact gacaagggtc tttgtggtgg
     2101 ttgtaacctc gtcaagacca tcaagtccat gaaccaccat tgctcttttg gttcccaggt
     2161 ttcgcaaaac ctctgccagc atggatacaa gtcccttgtc gaatacccca agcacctggc
     2221 ctttgacacc tgcaggattt gtaacgggac caagaatatt aaacacagtc cttatgccca
     2281 gttctctcct gacaagggca acattcttca tggaggtgtg gtaatacgga gcaaacagaa
     2341 atccaaagtt tgctttgttg acgctctcta caaaaacctc aggagtcatt tgaatcttta
     2401 tgccgagagc gttaagcaca tcagcacttc cactcttgct cgatattgcc ctgtttccat
     2461 gctttgcaac ctttatacct gcacagctgg caataattgc actggcggtg gatatgttga
     2521 aagtcctgcc gccatcacct cctgtgccac aggtatcaat ggcataatcc tcaatctcca
     2581 ccttaagggc actgttcctc attgccacgg cacatccggt tatctcgttg accgtctcgc
     2641 ccttcattct tagtgcagca agaaacgcac ctacctgaga ggtggtggcc tctcccttca
     2701 ttattgcgtt cattgaatct acagcttccg aatagctcaa atcctcacgc atgattatct
     2761 ttttaagcat tgcatcaagc attcttaatc cctcctacaa aattcccaat aatcttcttc
     2821 cctttctccg taattattga ctcaggatgg aactcaagcc cgtaaacagg aagactttta
     2881 tgcctgacag ccattatcgt tccttccaag gtgtgagcac atacttcaag aacatcaggc
     2941 agactactct cattcagctt gaaagagtgg tatctggctg taaggattgg attcttcaca
     3001 cccttgaata tgccttttcc atcatgaaga acaatggatg tcttgccatg catgcccttg
     3061 acatcaggca aaacaccgcc tccataagca caacctataa cttccattcc cagccctatt
     3121 cccaatatgg ggatttgcga agacaactcc tttacaatac caacgcagtt gcctgcatct
     3181 ttcgctcttc ccggacctga ggacataact attcccttta cattcatgtc ctgtatttca
     3241 gatatggcca ccaaattgct caccacccgc acatttggaa aaacctctcc cacgcactga
     3301 tagatgttgt acgcaaagga atcaccatta tcaataacca gtatcatccg agcacctcct
     3361 tcaatgcctt ggccttgttc tgtatctctt catactccct ttcaggacag gaatcataaa
     3421 caatgccgca tcctgcctgt atgcaagcct ttctatcctt gaaaacaatc gtccttatgg
     3481 caatacacat atccatgttt cctgaaaagt cgaagtatcc aatactgcct gcataaatgc
     3541 ccctgcttac attctccagc ccatcaataa tttccatggc tcttggcttt ggagctcctg
     3601 agacagttcc tgctggaaga caggcgtaaa gggcatcaaa acaatctttt ccttcctgca
     3661 gctgaccgga tatggtggaa acaatgtgca tgacatggga atacatttct atctccatgt
     3721 atttgtcaac ctttacagac ccaaacttgc acacctttcc aaggtcattt cttcccaaat
     3781 ccacaagcat gacgtgttcc gcccgttctt ttgcgtcatt caaaagctct tctctgagaa
     3841 gattgtcctc ttcctcattc cttcctcttt tccttgttcc ggcaattgga tttgtggcaa
     3901 ccacaccgtc ctttacctta accagacttt ccggagagga acccacaacc tgaaaatctc
     3961 caaagtcaat atggaacata tatggggagg ggtttagggt tctcaatttc ctgtatgcat
     4021 caaaaggttc aatatttgtt tcctgtataa atctttgaga taaaacaacc tggaacacat
     4081 cccccaactc aatatatttt tttgccttgt caacaacttc gacaaattct tgttcgtcaa
     4141 agtttgactc aaaccctttg gtttggaaat aagcagactc tgtaatacaa gcattactgc
     4201 tttgtatctt ttctttaata tccaaaagct tttttacaat ttcgtcgtaa gatctgtcat
     4261 caccaggcag cacattgtgt atgatgtata aacgatgagt aaagtggtca tacacaagca
     4321 cttccttata gagcatcagg tacgcatcag gaagatttaa gtcgtccttg ttgtttgact
     4381 tgatattcaa atactgcctt gccacatcat atccgacata tcctacagca ccaccaaaga
     4441 aaggcagacc atgttttgtt tcagatactt catacctccc cattacctct tttaacacat
     4501 ccagtacact gcctataaat ttcctatcgc ctacccagac ttcctcacca taacttttta
     4561 tggtcaagta tggagtaaag ccaagaagag aataccttcc ccagctcttg ccgcactctg
     4621 cgctttctag aagaaacttt ttctctccct taaggctgta gaacaaacct ataggagtca
     4681 tcaaatccgc attcatttcc agtttgactg gaatatatga agcggaattt cttttcaaat
     4741 cgaggaactc ttccttggtg ggataaatca ttagtatcac tcctttaaga tttttgtgta
     4801 taaaaaaagg gcagctcatc ccatgggacg agttaccctc gtggtgccac ccaatttcag
     4861 ctgtctggca aagctacttt aaaaaaaggc atttcatcct tttaggacga aatgccaata
     4921 atttcgttag ccacccaaac agccatctca ttccaggtac ggaaagtttc actttcgata
     4981 ccctgtcctt ttaacggtgg acttccggtt aatgctactt caaggttcgc acaacttctc
     5041 ccaggcccat tcagctgcac ttaaggtacc gcattcccac caccaacggc tctctgaaac
     5101 catccatcca gcctactctt cctgttcata gaatttggct gtattaaatt tccgatatgg
     5161 tacaacatat atcctataat gtcaataggt atttaaaaac ttttctgtat attatatgcg
     5221 aatcttttcg ccattgtata ctttaacagt ttcaacaaaa gcaagaatat tttcaaaggg
     5281 aacctcaggt tccaatacat gggtaggagc aagaaccaaa ccgccattgt cttctcttgc
     5341 cttgtcaatc atcttcatgc aaaaatctct aacttctgaa ggagtgccaa aaggcatcag
     5401 cgtctgagtc cccaaagtac cccataaagt caatttgtcc ttgtattcat ccttaatcct
     5461 taacggatcc atgcactctg gctgtacagg gttgagtatt tcaacgccaa tctctatcaa
     5521 gtcaggtata atcttcgtta gatttccatc tccatggtag aaaataaata tgtcaggatt
     5581 aatctctttt gcgcaagcta taactttttc caacctgccc tttaaatact ttttccacat
     5641 atccggggac atcatcatat ccagttgtgt agaaacatca tcaccaagcc ttaatatgtc
     5701 gcagcctgct tttgcgtaca ttctggcaaa cgtgcatttt atctctgtaa ttctgtccaa
     5761 aagacaattt gccatatcct catt
//
LOCUS       DAONVY010000080         5691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10470_length_5691_cov_3.923705, whole genome shotgun sequence.
ACCESSION   DAONVY010000080 DAONVY010000000
VERSION     DAONVY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10470_length_5691_cov_3.923705"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLI11_03320"
     CDS             <1..349
                     /locus_tag="PLI11_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPZ51925.1"
                     /translation="KMVGTALKASHFLKEKGYNVNVFNARFANPLDVSGIKDLVSKAK
                     CYMTLEDGVKVSGFGSSVVCQMAEEGIQKPCRIIGFPSSPIPHGSIDALFKRYGMDVQ
                     SIADAAIDMIEKL"
     gene            390..1235
                     /locus_tag="PLI11_03325"
     CDS             390..1235
                     /locus_tag="PLI11_03325"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPZ51926.1"
                     /translation="MNTIRIGIICNKNNKQCDDTVRQLALAIEREGAIPLCYGDDTYV
                     YDNADVLVSIGGDGTFLKVASQALKRDIPVIGINLGTLGFLTEIDKHDIDETARRLVR
                     NDYIVEERKVMKIVVTRDDVEVFSCHVINDCVIAREILSKVLYLEVYANSHYVDTYPG
                     DGVVLSTQTGSTAYSLSAGGPIVEPGNEVMVFTPLNAHMVEGRSIILGKSSVIEVKLH
                     REHESAYAICDGHLSYRLEKDDVVKCCLVDSHIKIIRMDPPDFYSVLRRKNAERQTKN
                     GNAQT"
     gene            1248..2939
                     /gene="recN"
                     /locus_tag="PLI11_03330"
     CDS             1248..2939
                     /gene="recN"
                     /locus_tag="PLI11_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPZ51927.1"
                     /translation="MLVQLNIKNIALIDDISIEFKPNMNCLTGETGAGKSIIIDSIGC
                     VLGGRTSRDTIRTGADSGSVEAVFFVNEPFVSEKLEELGIEEEEDHTLLLFREITSSG
                     RNICRINGRTVTLSILREIGEVLIDIHGQHDNQSLMRVNSHILLLDMYAGAKLSKVLI
                     EYGEKLSKFNEIHNALGNLAGDPKARERTIDLLKYQIDEIENAKLYENEDEELRTKKN
                     ILSNMERIVTSLNDAYQDLKSENSVLDKLENALQAVSRIEDFGDAYKEIMSRLEAVFY
                     EVEDISNAVRNEKDNMDLDMSELDSIEERLDIINRLKRKYGGTIEDIIRYKEEAQEKL
                     NELLDSEYMIEKYTKEMDKLSEELFDLCKKLDELRKKAAVDLAKNIVEQLHDLEMKNA
                     SFEVKIDFDESSNELGYPNFKKNGLNYVEFLVSANLGEPTKPLARIASGGELSRIMLA
                     IKTILADVDNIPTLIFDEIDIGISGQAARKVGEKMKLISKKHQVICVTHLAQIAAIAD
                     NNIYISKTTVKDKTITEAKTLSFDEKVEEVGRLLDGNAENEITKAHARKMIEEYI"
     gene            3054..4277
                     /gene="spoIVB"
                     /locus_tag="PLI11_03335"
     CDS             3054..4277
                     /gene="spoIVB"
                     /locus_tag="PLI11_03335"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HPZ51928.1"
                     /translation="MGLPRWKKTAVILFGVILPFISALLIFNFFLLPDSLTIVEGSDL
                     DNSSFVSVNASADGVGATAKAFGVLPIKNLEVNVVPGKKVYVSGTPIGIKLKSRGILI
                     TGYIGFLSKENIYVTPGRDSGFRIGDRILEINGKEVNDCDELISLIEESNKEEHTFTI
                     ERMGTIMKIVSKTCIDGQSGKMRLGLWIKDSAAGIGTLTFYDKKTGFFGALGHGISDN
                     NTARIFEVSEGEMYKATILDITKGIPGCPGELKGCFTDTERVIGNVFVNSETGIFGIM
                     SKEGFADIAGREMYIGSYNQVREGKAVILSTIGDGKPKEYEIEITKVNKTKVKSPKGI
                     VLKITDKELLDATGGIVQGMSGSPIIQNGKLVGAVTHVMVNDPSTGYGIFIEGMLANY
                     DLVYKEKGSCLDLAS"
     gene            4375..5600
                     /locus_tag="PLI11_03340"
                     /pseudo
     CDS             4375..5600
                     /locus_tag="PLI11_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254713.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
BASE COUNT         1873 a          887 c         1435 g         1496 t
ORIGIN      
        1 gaaaatggtt ggaactgcat tgaaggcatc acattttctt aaggaaaaag gatataatgt
       61 gaatgtattc aatgcaaggt ttgcaaatcc attggatgta tcggggataa aggatcttgt
      121 atcgaaggca aagtgctaca tgacccttga ggacggagtc aaggtctcgg gttttggcag
      181 cagcgttgta tgtcaaatgg cagaagaagg catacagaaa ccatgccgga ttattggatt
      241 cccatcaagt ccaatacctc atggaagcat tgatgccctt tttaaaagat atggcatgga
      301 tgtgcaaagt attgccgatg ctgcaataga catgattgaa aagttgtaat agataaatta
      361 taatttctga cagaacttga ttcagggagt tgaataccat caggattgga ataatttgca
      421 acaagaataa taaacaatgt gatgatacgg taagacagct tgccctagca atagaaaggg
      481 aaggcgcaat ccctctttgc tatggtgacg atacatatgt gtatgacaat gccgacgtgc
      541 ttgtaagcat tggtggagac ggtacctttt tgaaagttgc ttcacaagcc ttgaaaagag
      601 atatcccggt aatagggata aaccttggca cgcttggttt tcttactgaa attgacaagc
      661 atgatattga tgaaactgca agaagattag tcaggaatga ctatattgtt gaggaaagaa
      721 aggtaatgaa aatagttgta acaagggatg acgttgaggt cttttcatgt catgtcataa
      781 atgactgcgt tatagctcgg gaaatactgt ccaaggtttt gtatcttgaa gtatatgcaa
      841 acagccacta tgtggacacc tatccaggag acggtgttgt tctgtccaca cagacaggct
      901 cgacagcata ttctttgtcc gcaggaggtc ctatagtgga gcccggcaat gaagtaatgg
      961 tgtttacacc cttgaatgca cacatggtgg aaggcagatc aataatactt ggcaagagtt
     1021 ctgttataga ggtaaagctc cacagggagc atgaatctgc ctatgccatt tgtgatgggc
     1081 acctttcata caggttggaa aaagatgatg ttgtaaaatg ttgtcttgta gacagtcaca
     1141 taaagataat caggatggat ccgcccgact tttattccgt cctaagaagg aaaaatgcag
     1201 aaagacagac gaaaaacggt aatgctcaaa cctaaagggg gtatagtgtg ttagtacagc
     1261 ttaacataaa aaatattgcc ttgatagatg acatttcaat agagttcaaa ccaaatatga
     1321 actgtcttac tggagagacc ggggctggaa agtcaataat tattgactcc attggttgtg
     1381 tgcttggagg acggacttca agagatacta tacgcacagg tgctgacagt gggtccgtgg
     1441 aagcggtatt ctttgttaat gagccctttg tgtctgaaaa actggaggag cttggtattg
     1501 aagaggaaga ggatcatacg cttcttttgt tccgtgagat tacaagtagc ggaagaaata
     1561 tatgcaggat aaacggtcgg acggtaacct tgtccatact tagggagata ggagaggtat
     1621 tgattgacat acatgggcag catgacaacc agtccttgat gagggttaac tcacatatac
     1681 tattgctgga catgtatgca ggagcaaaac taagcaaggt tttgattgag tatggcgaaa
     1741 aactctcaaa atttaatgaa atacataacg cactgggcaa tctggcggga gatcctaaag
     1801 caagagaaag aaccatcgat ttgcttaaat atcagattga tgagatagag aatgcaaagc
     1861 tgtatgaaaa tgaggatgag gagttaagga ccaaaaaaaa tattttatca aatatggaaa
     1921 ggattgtaac gtctcttaat gatgcttatc aggatctaaa atcggagaat tcagttttgg
     1981 acaagctgga aaacgcattg caggcagtgt cgaggattga ggattttgga gatgcttaca
     2041 aggaaattat gtccagactt gaggcagtgt tttatgaggt tgaagatatt tcgaatgctg
     2101 ttagaaatga aaaggataat atggatcttg acatgtctga gttggattcc atcgaagaga
     2161 gactggacat tattaacagg ctgaagagaa aatatggtgg tactattgag gatataataa
     2221 ggtataagga agaagctcag gaaaagctta acgagctttt ggacagcgag tatatgattg
     2281 aaaagtatac aaaggaaatg gacaagttgt ctgaagaact ttttgatttg tgcaaaaagt
     2341 tggatgaact tagaaaaaaa gccgctgttg atttggcaaa aaatattgta gaacaattgc
     2401 atgatttgga aatgaaaaat gcatcctttg aggttaaaat agattttgat gagagtagta
     2461 atgaactggg atatcccaat tttaagaaaa acggactcaa ttatgtggag ttcctggttt
     2521 ctgcaaatct gggagaaccc acaaaacctc tggcaagaat agcatccggc ggcgaattgt
     2581 ccagaatcat gctggcaata aagaccattc ttgctgatgt ggacaatatt ccgactttga
     2641 tatttgatga aattgacatt ggtataagcg gtcaggctgc aagaaaagtt ggtgaaaaaa
     2701 tgaaacttat ttccaaaaag catcaggtta tttgtgtaac acatcttgcc cagattgcag
     2761 ccattgctga caacaatatt tatatcagca agacaactgt gaaggataag acaattacag
     2821 aggcaaaaac tttaagtttt gatgaaaagg ttgaagaagt tggaagactg ctggacggca
     2881 atgcggagaa tgagataacc aaagcacatg ccagaaaaat gatagaggaa tacatctaaa
     2941 caattttatc tatagcaata tttatattgt ctaaaacaaa cctcctcgga gataatagct
     3001 tatggtgaaa catatacatg tgttttccac aataaagcag gggaggttaa ttaatggggt
     3061 tgcctcgatg gaaaaaaaca gctgttatat tgtttggcgt gattttgccc tttatatcag
     3121 ctttattaat ttttaacttt tttttattgc cggactctct gaccattgta gaaggctctg
     3181 acttggataa cagctctttt gtatctgtga atgcatcggc ggacggtgtt ggtgcaacag
     3241 caaaagcttt cggagtcctt ccaatcaaga accttgaagt aaatgtggtg cccggtaaaa
     3301 aagtttacgt cagtggaact cccattggca taaagctcaa gtcaaggggc attttgataa
     3361 ctggatatat agggtttttg tcgaaggaaa acatctatgt aactccggga agagacagtg
     3421 gtttcaggat aggtgacaga atccttgaga taaacggcaa agaggtcaat gactgtgatg
     3481 agttgatttc tttgattgag gagagcaata aggaggaaca cacctttact atagaaagaa
     3541 tgggaaccat aatgaaaatt gtcagcaaga cctgcattga tggtcaaagt ggcaaaatgc
     3601 gtctgggact ttggataaag gacagtgccg ctggcatagg cacacttacc ttttatgaca
     3661 agaagacagg cttctttgga gctcttggac atggaatatc cgataacaac acagcgagga
     3721 tatttgaggt ctcagaggga gagatgtaca aggccaccat actcgacata actaaaggga
     3781 tacccggatg tccaggagaa ctcaagggat gcttcactga cactgaaaga gtaattggca
     3841 atgtctttgt aaacagcgaa acaggtattt ttggcatcat gtcaaaggaa ggctttgcag
     3901 atattgcagg cagggaaatg tatatcggat cctacaacca ggtcagggaa ggcaaggctg
     3961 taattctgtc gaccataggt gacggaaagc cgaaagagta tgagatagag ataacgaagg
     4021 taaacaagac gaaggtaaag agtccaaaag gtattgttct aaagattacg gataaagaac
     4081 ttttggatgc tacaggaggt attgttcagg gcatgagcgg aagcccgata atccaaaacg
     4141 gaaaacttgt aggcgcagtg actcatgtca tggtcaacga tccaagcacg ggatatggaa
     4201 tattcataga ggggatgctt gcaaactacg acctcgtata caaagagaaa gggtcctgtc
     4261 ttgacttggc atcataatga tattgtggct gtcgcaataa tgcggcagct ctttttttga
     4321 ttgaaaaagt aagaaaaaag tgctattcta tacaaaaagg tcaaggagac gtatatgctt
     4381 aagtgtgaag ttatatctat aggcacggag cttttaatgg gtcagattgt aaacacagac
     4441 gcccaatata tttcaagtag gcttcctgaa tgcggtgtgg gagtgtttta tcacagtgta
     4501 attggtgata atcctggaag aattaaggaa gctttgaaaa ttggttttga aagatgcgat
     4561 gttgtaataa ccacgggcgg acttggccca acacaagacg accttacaaa agagattgtg
     4621 gcggattttt taggtctgaa attagttctt gatgatagaa gcagcgaaag aataaggaaa
     4681 tactttgaag acagggggag ggttcttgtt gaaagcaacc tgagacaggc gtattttcct
     4741 gaaggcagca taattgttga aaacgacatg ggaaccgcac cgggatgcat tgtggaaaag
     4801 gacgacaagg tgattgtaat gctgcctggc cctcccaagg aactaatacc catgtttgac
     4861 aaggtaatga aatacttcaa agaaaaacaa ttgggaaggc tttttcgaaa tatataagaa
     4921 tagtaggcat gggcgaatcc atggtagagc aggaaatata cgatattgtg tcgtcccaaa
     4981 cgaatcccac catagctact tatgcaaagg acggcatagt taccatcagg gttactgcca
     5041 gtaatgaagg aggcagcaat ccagaggttt tggtaaatga tatggtgaat aagatatgtg
     5101 aaatattggg tgacaatgtt tatacaacgg aaaatgatga attggagtat gttgtcttca
     5161 agcttctcaa ggaaagaaat ttgaaaattg ctaccgcaga atcctgttca ggaggcgtgc
     5221 tggcacagtt gctgacatcc atacctggaa gttcgcacgt ttttcacagc ggatttgtta
     5281 cttacagtga agaatctaag atccgtgcac tgggagttga aaggagtacc atagaaactt
     5341 acagtgcggt aagcagtcag actgccaggg aaatggcaga tggagcatgc aggaaaacag
     5401 gatgtgatgt gggagtatcc attacagggt atgcaggacc cgatggcgag tctgtgggac
     5461 ttgtgtatat aggggtttca tatggtggtg ctactgaggt gtccgagttc cgcttttcgg
     5521 gcaaccggga aagaataaga actatgagtg ctgttactgc gctggatatg gtaagaagaa
     5581 caattttgaa taaaagttaa ggaaatgagg caaaataaca caaccataac atgagaaagg
     5641 ttgtgttttt ttgtatgaag gcagttatca tggctggagg tgaaggtgtc a
//
LOCUS       DAONVY010000081         5678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10491_length_5678_cov_3.255202, whole genome shotgun sequence.
ACCESSION   DAONVY010000081 DAONVY010000000
VERSION     DAONVY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10491_length_5678_cov_3.255202"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLI11_03345"
     CDS             complement(<1..664)
                     /locus_tag="PLI11_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPZ51929.1"
                     /translation="MRINNNLMAMNAQRYLGVNNANIQKSIEKLSSGFRINRAGDDAA
                     GLAISEKMRSQIRGLKMASKNSSDAISLIQTAEGALNETHAILQRMRELAVQAATDTN
                     ESVDRNALQNEIDELKKELTRISQQTEFNEKKLLNGDRGYKYQGDLSDTAIANISIGV
                     LDTPVPEITVKQEYVVDADTEDKKLTFTISARVESGEDDVISFTYDDTTGTWTRETNS
                     VTD"
     gene            complement(794..1000)
                     /gene="csrA"
                     /locus_tag="PLI11_03350"
     CDS             complement(794..1000)
                     /gene="csrA"
                     /locus_tag="PLI11_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010302159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPZ51930.1"
                     /translation="MLVLSRKKGESLIIGDDIKIKILECDNDRVSIGIDAPKEIPIIR
                     GELYEDTAKSNIEAISSMFSLGDL"
     gene            complement(972..1457)
                     /gene="fliW"
                     /locus_tag="PLI11_03355"
     CDS             complement(972..1457)
                     /gene="fliW"
                     /locus_tag="PLI11_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPZ51931.1"
                     /translation="MVIQTTRFGEIEVDEKKIIEFEKGIPGIEELKRFIFLTPKESYP
                     IFFLQSIDEKDIALPVVNPYDFKADYNPKVKESVFKEIEIESDEDILVLNVAVVPKDL
                     KKMTVNLAAPILINIKKFKAKQVILDDKQYSIREPMYNSLVTLIQQGGQNNAGTVAKE
                     G"
     gene            complement(1469..2074)
                     /locus_tag="PLI11_03360"
     CDS             complement(1469..2074)
                     /locus_tag="PLI11_03360"
                     /inference="COORDINATES: protein motif:HMM:NF039791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HPZ51932.1"
                     /translation="MIGRIQIRTTMGQIGIRTEKANIEIENSLPKMTIRRKQAEFRIK
                     SKPSKFKLDQSQYLVASGNMTTLFLSSTRFQKARQKTLDAIRRIAEEGDLLMEIEKKN
                     NPIPEIALKNTIKETSVNIAKFPQNKIIWEKGYFELEWTPHELEFDWEINKPVIKASR
                     PKVEIYMKRWPSIEISVVTDTEPLIEKYIEKKIGKNIDRKA"
     gene            complement(2089..3012)
                     /gene="flgL"
                     /locus_tag="PLI11_03365"
     CDS             complement(2089..3012)
                     /gene="flgL"
                     /locus_tag="PLI11_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HPZ51933.1"
                     /translation="MRITNSMLVRNMMTNLKRSMSRMHQYESQLSTGKRITKISQDPV
                     GVIKSLQARKNLSKSIQYKENAEDAQAVLDFTETALMEINNLLKRAYELTVNAPNDTN
                     TPSEREAIAVEIDQIRHQVINVLNSTYGSQHIFGGYNVATAPFREIGGTFLYNGEDLT
                     NGDLITLDAFSKESIEYLVGNNVSMDVTMSGARVVGYGENNMIQMLADLSNSLRNDDP
                     VDSFVGRLQESQEHILALVAEIGGKSNRIQSVIDRFDADIITYEDVKARMEDIDQAET
                     IMYFKMEEAVFRTALAVGARVIQPSLVDFLR"
     gene            complement(3047..4534)
                     /gene="flgK"
                     /locus_tag="PLI11_03370"
     CDS             complement(3047..4534)
                     /gene="flgK"
                     /locus_tag="PLI11_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPZ51934.1"
                     /translation="MSSTFYGFEIAKTGLRYNQKALDVTAHNIANVETEGYSRQRIES
                     SSIDPYVTQTLVAPLTNALVGGGVSIDYIQQIRNQFLDRQYRNEASLMGEWTVKAEAL
                     SYIEKLLQEPSDIGVMSIIDRFFVGMQELASNVGSKDVRTNFLQQAITLTDTINYYGS
                     QLNQIQDEQDYAFTTSVKQVNEYIDKILAYNKSIARYELGGNVANDLRDKRNVLLDKL
                     SEYANITYVENKVGNELRLTVNMTYFDENGDRQEVVLIDHDQANKLEMYNKPGSDYHY
                     IRVDGIDMDNLESGKLKGYLDIRDGNTDEVKGIQYYKDKLDYFANSLVNGINEIHRKG
                     WTIPQGGGSSMTGIDFFDPSGIDALSIKVNDEIIKDVFKIAASDAEVVGSINSGNNKI
                     ILELINLREQAAIGGSDNFEAYMSSYIAELGIDTAYASTMRDNEEALLNSVMEQRMSI
                     SGVSIDEEMANMVKFEKSYQAAARILTALDEMLDVLINRVGLVGR"
     gene            complement(4544..5053)
                     /locus_tag="PLI11_03375"
     CDS             complement(4544..5053)
                     /locus_tag="PLI11_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HPZ51935.1"
                     /translation="MEYRELVGKLTESLEKQKAYYEAINELSGQKTEVVLNRDIQSLN
                     KIVEAEEVLLVKIGQEEQKRMEIASSLAKSLGMSEEDITISGLVRELKDRNEDTTTIE
                     EVSEGLVDVIEKQKVYNKTNMRLIENNLNYIKEVIQKVVIGDGSENIYNPKGQRTITQ
                     KTNIIDESV"
     gene            complement(5068..5355)
                     /gene="flgM"
                     /locus_tag="PLI11_03380"
     CDS             complement(5068..5355)
                     /gene="flgM"
                     /locus_tag="PLI11_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPZ51936.1"
                     /translation="MKINPTNPTDPISAYKTSQIKKANVNDGFEINDKLDLSDNAKIY
                     SKAIKAIKDIDEVRYDKVNEIKERIEKGIYTIDSGKIAEKMIKGTRIDEKA"
     gene            complement(5371..5592)
                     /locus_tag="PLI11_03385"
     CDS             complement(5371..5592)
                     /locus_tag="PLI11_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51937.1"
                     /translation="MEGNKELKCESCDKPIDSGRYCLECTVKKHNELSYLKSNLRPGE
                     TATGRSQSATGKERMHYIPRSKKKDDEEE"
BASE COUNT         1417 a         1347 c          932 g         1982 t
ORIGIN      
        1 tatcagtaac actattggtt tctcttgtcc aagtgccagt agtatcgtca tatgtaaatg
       61 atataacgtc atcttcacct gattcgacac gagctgagat agtaaatgtt aacttcttat
      121 cctccgtatc ggcatcgaca acatattctt gtttaacagt aatttcaggc actggtgtat
      181 ctaacacgcc aattgaaata ttggcaatgg cagtatcact caaatctcct tgatacttat
      241 aaccacgatc accattcaac agctttttct cgttgaactc ggtttgctga gaaattcttg
      301 taagctcttt cttaagctca tctatttcat tctgcaatgc atttctgtca actgactcat
      361 ttgtatcagt tgctgcctga actgcaagtt ctctcattct ctgaaggatt gcatgggttt
      421 cgttcaaagc accttctgca gtctgaataa gtgaaattgc gtcggatgag ttcttggaag
      481 ccatcttaag accacgaatc tgagatctca tcttttctga tatggctaat cctgctgcgt
      541 catcgccagc tctgttaatt ctgaagcccg aagataactt ctcaatggat ttctgaatgt
      601 tggcattgtt aactcccaaa tacctttggg cattcattgc cattaagttg ttgttaattc
      661 tcattataat tcctccttga aatttaatat tcggcatcct tgcctgtatt taatgtatcg
      721 aacagaaaca tctttggcgc cttcgtgatg tttctccgtt caatacatat tatcggaaag
      781 tattgtaaaa actttagaga tcacccaatg aaaacattga agatattgcc tctatatttg
      841 atttggcagt atcttcatac aattcaccgc gtataatggg gatttctttt ggagcatcaa
      901 taccaatgct gaccctgtca ttatcgcatt ccagtatttt gattttaatg tcatccccaa
      961 taatcagaga ctcacccttc tttcgcgaca gtaccagcat tgttttgtcc tccctgttga
     1021 attaatgtaa cgagactatt gtacattggt tcccggatac tgtactgttt gtcatccagg
     1081 ataacctgct ttgccttgaa cttctttatg tttatcagta ttggtgcagc cagattaaca
     1141 gtcatcttct ttaaatcttt tggaacaacg gctacattca aaaccagaat atcttcatca
     1201 ctctcaatct ctatttcctt aaaaaccgac tctttaacct ttgggttata gtcggcttta
     1261 aagtcataag gatttactac agggagagca atatcctttt catctatcga ttgtaggaaa
     1321 aatatcgggt agctttcctt tggagttaaa aagataaatc ttttgagttc ctctatgcca
     1381 ggaatcccct tttcaaactc aataattttc ttctcgtcaa cctcaatttc tccaaacctg
     1441 gttgtttgta taaccatttt agtcactcct atgcttttct atcaatattc ttgccaatct
     1501 tcttctcaat atatttctct ataaggggtt cagtatccgt aacaacggaa atctcaatgg
     1561 aaggccacct tttcatgtaa atctcaacct tgggtctgga cgctttaatg actggcttgt
     1621 taatctccca gtcaaactca agctcgtgag gagtccattc aagctcaaaa tagccttttt
     1681 cccagataat cttgttctga ggaaacttcg ctatatttac gcttgtctcc ttgattgtat
     1741 tcttaagggc gatctcagga atgggattgt tctttttctc aatttccatg agaagatccc
     1801 cttcctcggc aattcttcta attgcatcaa gagtcttttg tcttgctttc tgaaatcttg
     1861 tagaagacag gaaaagagtt gtcatattac cagaagcaac aaggtattgg ctttggtcca
     1921 gcttgaactt ggaaggcttg gattttatac gaaactctgc ttgtttgcgc cttatagtca
     1981 ttttgggcaa agagttctca atctcaatat tggctttttc agttctaata ccaatctgac
     2041 ccatagtcgt ccttatctga atacgaccaa tcatgtcccc tcacctcttt atctcaaaaa
     2101 gtcaaccaaa gaaggttgaa taacccttgc acctactgca agagccgttc tgaaaacagc
     2161 ttcttccatc ttgaaataca taatcgtttc agcctggtca atgtcctcca ttctggcctt
     2221 cacatcttca taagtaatta tatctgcatc aaatctatct ataactgatt gaattctgtt
     2281 ggacttacct ccaatctctg ctacgagtgc aagaatatgc tcctgactct cctgcaatct
     2341 tcccacaaaa gaatccacag ggtcgtcatt tcttagtgaa ttagacaagt cagccagcat
     2401 ctgaatcatg ttgttttctc catatccaac aacccttgcg ccactcatgg ttacatccat
     2461 gcttacattg tttccaacca ggtactcaat ggattcttta ctaaacgcat caagagttat
     2521 taggtcaccg tttgtaaggt cctctccatt gtaaagaaat gttccgccaa tctccctaaa
     2581 aggagctgtt gccacattgt aaccaccaaa tatgtgctgg cttccgtatg tggagttaag
     2641 cacatttatg acctggtgtc tgatttggtc aatctctaca gcaatagctt ccctctccga
     2701 aggagtatta gtatcattcg gagcatttac agtaagctca taagctctct ttaacaaatt
     2761 atttatctcc atcaaagcag tctcggtaaa atccaaaacc gcctgagcat cttccgcatt
     2821 ctctttatat tgtattgact ttgaaagatt ctttcttgcc tgtagtgatt taataacacc
     2881 caccggatcc tgggatattt tagttatcct ctttccagta gaaagctgac tctcatactg
     2941 atgcatgcgt gacattgatc ttttcagatt agtcatcata tttcttacca gcattgaatt
     3001 agtaatacgc ataaacacac ctctttttaa tgtttttcac aatattttat cttcctacca
     3061 agccaaccct gttgattaat acatccagca tctcatccag agcagtcaga atcctggcag
     3121 ctgcctgata agacttttca aacttaacca tatttgccat ctcttcatca attgacacac
     3181 ccgagatgct catacgctgc tccataacgc tgttcaaaag cgcttcttca ttgtcgcgca
     3241 ttgtgctggc ataagcagta tcaataccaa gttctgcaat atagctgctc atgtacgcct
     3301 cgaagttgtc actgccgcct attgccgctt gttcccttaa atttataagt tcaaggataa
     3361 tcttgttgtt tccagagttg atagaaccca ccacttccgc atccgatgca gcaatcttga
     3421 acacatcctt tataatctca tcattcacct ttatactcaa tgcatcgata ccgcttggat
     3481 caaagaaatc aattccagtc atagatgaac cgccaccttg ggggatggtc catccctttc
     3541 tgtgaatctc gtttattccg ttgacaaggg aatttgcaaa atagtcaagc ttgtccttgt
     3601 aatattggat gcctttaacc tcatcggtgt ttccatctct aatatccaag taacctttaa
     3661 gctttcctga ttcaaggttg tccatgtcaa tgccatcaac cctgatatag tggtagtcac
     3721 tgcctggttt attgtacatc tccagcttgt tagcctggtc gtgatctata aggacaactt
     3781 cttgtctgtc tccattttca tcgaaatacg tcatgttgac agtaagcctt agctcattgc
     3841 caaccttgtt ctctacatat gtaatgtttg catactctga gagcttgtca agaagcacgt
     3901 ttctcttgtc tctcaagtca tttgccacat tgccaccaag ctcgtatctg gctatgcttt
     3961 tgttatatgc caaaatctta tcaatatatt cgttaacctg ctttacagat gtggtaaaag
     4021 cgtaatcctg ttcatcctgt atctgattca gctgacttcc atagtaattg atggtatcag
     4081 ttagagtgat tgcctgctgc agaaagtttg tccttacatc cttgcttcct acattggatg
     4141 caagctcctg catgccgaca aagaatctgt caattatact cataacccct atatcggaag
     4201 gctcctgcag cagtttctca atgtagctca aggcctctgc cttgacggtc cattcaccca
     4261 tcaaggaagc ttcatttctg tactgtctgt caagaaactg gtttcttatc tgttggatat
     4321 agtcgatgct gactccacca cctacaagag cgtttgtcaa aggagccacc agtgtttgag
     4381 taacgtatgg atctattgaa gaactttcaa ttctttgcct tgaataacct tctgtttcaa
     4441 cgtttgctat gttgtgtgct gttacatcca aagccttctg attatagcga agtcctgttt
     4501 ttgcaatctc aaaaccgtaa aaagtagaac tcataatttc ctcctatacg ctttcatcta
     4561 ttatgttggt tttctgtgta atggttcttt gtcccttggg attgtatatg ttctcgcttc
     4621 cgtcccctat aacaaccttt tggattacct cttttatgta attcagattg ttctctataa
     4681 gccgcatgtt cgtcttgttg tataccttct gtttttcaat aacatccaca agaccctcgc
     4741 tgacttcctc aattgtagtg gtatcctcgt tcctgtcctt gagctctctt acaagaccgg
     4801 atattgtaat gtcttcttcc gacatgccaa gactctttgc aagactgctt gcaatctcca
     4861 tcctcttttg ctcttcctgc cctattttga caagaagcac ctcttcagcc tcaactatct
     4921 tattgagaga ctgaatgtct ctattcagga ccacctccgt cttttgtccg gatagctcgt
     4981 ttattgcttc gtaatatgct ttttgctttt ccaacgattc ggtcaatttc ccgacaagtt
     5041 ccctgtactc catttaatca ccttcagtta tgccttttca tcaatgcgtg ttccttttat
     5101 catcttctcc gcaatcttgc cgctgtctat ggtatagatg cctttctcta tcctttcctt
     5161 tatctcatta accttgtcgt atcttacttc atcgatatcc ttgattgcct tgattgcctt
     5221 tgaatatatt tttgcgttgt cggacaaatc cagcttgtcg tttatctcaa acccatcatt
     5281 tacatttgcc tttttaatct gacttgtctt gtatgcggaa atgggatccg taggatttgt
     5341 aggattaatt ttcatgctga caccttcttt ctactcttct tcatcatctt tcttcttgct
     5401 ccttggaatg taatgcattc tctccttgcc tgtcgcactt tgagatctgc ctgtagctgt
     5461 ttctccaggc ctcaaattcg atttaagata tgaaagctcg ttatgtttct ttacagtgca
     5521 ttcaaggcag tacctgcctg aatctatggg cttgtcgcag gactcgcatt tcaactcttt
     5581 gttgccttcc atgctaagcc ttccctcttt gagcaggagc agtattattt tttcattaac
     5641 ctcagtctcc tcactcaact cttatatagt tgccagcc
//
LOCUS       DAONVY010000082         5666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10522_length_5666_cov_3.257352, whole genome shotgun sequence.
ACCESSION   DAONVY010000082 DAONVY010000000
VERSION     DAONVY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10522_length_5666_cov_3.257352"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLI11_03390"
     CDS             complement(<1..719)
                     /locus_tag="PLI11_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPZ51938.1"
                     /translation="MRIGAQLYTVRDYCKTEEEFRNTLKKLAEFGYEYVQISGVGLEP
                     KVIREVCDEVGMKIRLTHTNPDRLLNDPMAVIEEHKIFGCNYVGIGAMPERYKGSYEG
                     LKDFIKDYSEVAKVLKENGMSFHYHNHSFEFEKFEGRIGFDIMLEETVEGDWYFIFDT
                     YWAQHGGVCPTDYINRMEGRIKALHFKDMRMEGSNQKFAAIGSGNLNWDKIIDACKKT
                     NVEYAFIEQDTHFVDDAITELG"
     gene            complement(746..1819)
                     /gene="uxuA"
                     /locus_tag="PLI11_03395"
     CDS             complement(746..1819)
                     /gene="uxuA"
                     /locus_tag="PLI11_03395"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPZ51939.1"
                     /translation="MKMSLRWFGSKYDTVKLEYIRQIPGVDGVITTLYSKMPGELWTF
                     EEILELKKEVEEAGLKIHGIESVNVSDAIKAGTKERDQHIENYIKTLENLGKADIHMV
                     CYNFMPVFDWTRTELSRKLPDGSTVLAYTQEAVDSINPEEMFNAIEGERTGAFMPGWE
                     PERMARIKELFELYKDVDDEKLFENLKYFLEAIMPVCDKYDINMAIHPDDPAWSVFGL
                     PRIIINKENILRMMKMVDNKHNGLTFCTGSFGTNLKNDMPDMIRSLEGRLHFAHVRNL
                     KFHSQTNFEESAHLSSTGTFDMYEIMKALYDIKFTGPIRPDHGRMIWGEVAMPGYGLY
                     DRALGATYLNGLWEAIDKCAKRC"
     gene            1982..2881
                     /locus_tag="PLI11_03400"
     CDS             1982..2881
                     /locus_tag="PLI11_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51940.1"
                     /translation="MLKLQVPTMAADDPRQYMLNENLEIYEKVGVPSGALEMHFHNFY
                     EIMYIIEGEFTILVNNTNYVLNSGDFILININNLHHYTYTDSKHENNKRILMWVSKKY
                     LNQLSAPSYELDSCFANGFTPAWRFPPHHREQLYEYMLQLLYISADDNRNKTENKLLQ
                     NAYTTLFFVHLNKLCQRKEFSFSPENTYNDPMIRTVSEYINQHIDEPITLDTLAAQVH
                     LSKYHFARLFKETTRMTVRDFIIQKRLILACEKIWAGEPLKNIYSACGFTDYSSFYRN
                     FKSFYGLSPSEYKEFYDKGPAND"
     gene            complement(2926..3762)
                     /locus_tag="PLI11_03405"
     CDS             complement(2926..3762)
                     /locus_tag="PLI11_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPZ51941.1"
                     /translation="MNIVYGAFADEYAVSLEEQIEGLIQNKIDYLEIRFVDGKNIADI
                     DLETVKRLNETLKENGISVFSIGSPIGKIDITDDFNEHLVKLERICESANIFGSKYIR
                     MFSFYNRNRHPIEEFRKTAFERVEKLLELADRYGVTLCHENEEGIYGESPEDCLELFE
                     SFEGRLRCVFDMGNFILAGYDPLQAYKMLCGYIEYFHIKDGTKDGRITVPGYGQARIK
                     EIVKDFAAKKEVVHVTLEPHLMSFVGLDRLTEGEFKREVSFKSNKEAFNFAAENLKRI
                     LS"
     gene            4711..5109
                     /locus_tag="PLI11_03410"
     CDS             4711..5109
                     /locus_tag="PLI11_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51942.1"
                     /translation="MIYLLLFTWCLPQTLLAFIIRAFVKPVVSEKRGNVNVYKVDWDT
                     GSLSMSNKVFLCKTHWENEKVLMHEIGHFKQSLILGWFFLPIIGLPSLMWAWWGHDLA
                     NKYREKKGLHKVDYYSFYTEKWANKLGGVD"
     gene            complement(5111..>5666)
                     /locus_tag="PLI11_03415"
     CDS             complement(5111..>5666)
                     /locus_tag="PLI11_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPZ51943.1"
                     /translation="ERLDIPIEEFGDYALKATTPVRIAGRCAVFAESDMIHKQQLGYY
                     EADIIGGLCEALVRNYLNNIGKGKEILPKVFFQGGVAANKGIKKAFEDALGFEVIVPD
                     YYDMMGAIGAAIIARNAVRKTGRTNFKGFGLADSTFVSKSFECAGCPNCCEVVRIYEN
                     EKAIGCFGDRCGKWNTSLPAQTIA"
BASE COUNT         1582 a         1337 c          938 g         1809 t
ORIGIN      
        1 ccaagttcgg ttatagcgtc atcaacaaaa tgtgtatcct gctctataaa tgcatactcc
       61 acattagtct ttttgcatgc gtcaattatt ttgtcccagt taagattacc acttccgata
      121 gctgcaaact tctgattcga tccttccata cgcatgtctt tgaaatgcag ggctttaatt
      181 ctgccttcca ttctattaat gtaatctgta gggcaaacac ccccatgttg agcccagtaa
      241 gtgtcgaata tgaaatacca atcaccttct actgtctctt caagcatgat gtcaaaccct
      301 attcttccct caaacttttc aaattcaaaa gagtggttgt ggtaatgaaa tgacatacca
      361 ttttccttta gaaccttggc tacttcgctg taatccttta tgaaatcttt aagaccttca
      421 taactgcctt tatatctttc tggcattgca cctataccta catagttgca tccaaaaatc
      481 ttatgttcct cgataacagc cattgggtca ttaagaagcc tgtcaggatt tgtatgcgtc
      541 agtcttatct tcataccaac ctcatcacaa acttccctta tcacctttgg ttcaagacct
      601 actcctgaaa tctgaacgta ttcataacca aattcagcaa gttttttaag agtatttcta
      661 aattcctctt ctgttttaca ataatccctt actgtataaa gctgtgcgcc tatacgcatt
      721 tttcatccct cctttaaaga taagtttaac atctttttgc acacttatct atagcttccc
      781 acaaaccatt caaataagtg gcacccagtg cacggtcata cagtccgtat ccaggcatag
      841 caacttctcc ccaaatcatt ctgccatggt ctggacgaat tggtcctgtg aattttatat
      901 cgtacagcgc tttcattatc tcatacatgt caaaagttcc cgtagatgac aagtgagccg
      961 actcttcaaa attggtctgg gagtggaatt tcagattgcg cacgtgagca aaatgaagtc
     1021 tgccttcaag cgaacgtatc atatcgggca tatcgttctt tagattcgtt ccaaaggaac
     1081 ctgtgcagaa ggtcaatcca ttgtgcttgt tgtccaccat tttcatcatg cgcaggatgt
     1141 tctccttgtt aatgattatt cttggcaaac caaaaacact ccatgcgggg tcatctggat
     1201 gtatcgccat gtttatgtcg tacttgtcac aaacaggcat tattgcttct aggaagtact
     1261 taaggttctc aaacaatttc tcatcgtcca catccttata aagttcgaac aactccttaa
     1321 ttcttgccat gcgttcaggt tcccagcctg gcatgaatgc acctgttcgc tctccctcaa
     1381 ttgcgttaaa catctcttca ggatttatgc tgtccactgc ctcctgtgtg taagctagaa
     1441 ctgtggaacc atcgggaagc tttctggaaa gctcggtacg agtccagtca aacacaggca
     1501 taaagttgta gcacaccatg tgtatgtctg ccttgcccag gttttcaagg gtcttgatat
     1561 agttttctat gtgttgatcc ctttcctttg taccagcctt tattgcatca cttacattga
     1621 cgctttcaat tccgtggatt ttcaagccag cttcctcaac ttcctttttc aactcaagga
     1681 tttcctcgaa agtccacaac tctccaggca ttttgctata aagggtggtt atgacaccgt
     1741 ctactcctgg tatctgtctg atatattcca gtttgacggt atcatatttg cttccaaacc
     1801 accttaatga cattttcata tttcttttct cctttcacct ttcagtatca tattaaatac
     1861 ccatttctct catgtattgg cactaatgta tatataatat agcatttttt ctctataata
     1921 atagcatatt ttgcttgttt aattgcaatt cttgctgtat tatatatcct gggtgataaa
     1981 aatgcttaaa ctacaagtac caacaatggc agcagacgat ccaaggcagt atatgttgaa
     2041 cgagaacttg gaaatatacg agaaagtggg agtccccagt ggagcattgg agatgcactt
     2101 tcacaacttt tatgaaataa tgtatattat tgagggtgaa ttcactatac ttgttaataa
     2161 cacaaattac gtcttgaact caggcgactt tattctcatc aatataaata atctccatca
     2221 ttatacctat actgacagca agcacgaaaa taacaaacgg attctcatgt gggtttccaa
     2281 gaaatatttg aaccaactgt ccgctcccag ttatgaatta gacagttgtt ttgcaaacgg
     2341 atttactcct gcgtggcgtt tcccacccca tcacagggaa caactctatg agtacatgct
     2401 gcaactcctg tacataagtg cggacgacaa caggaacaaa acagagaaca agcttctaca
     2461 gaatgcatac actacgctat tctttgtcca tctgaacaag ctctgccaga gaaaagagtt
     2521 ttccttctcc ccggagaaca cttacaacga cccaatgata cgcacagtgt ccgagtacat
     2581 aaaccagcat atcgacgaac cgataacatt ggatacattg gcagctcaag tgcacctgag
     2641 caaatatcac tttgctcggc tgttcaaaga gaccacgcgc atgaccgtca gggatttcat
     2701 aattcaaaaa aggctgattc ttgcctgtga gaagatttgg gcaggagaac cacttaagaa
     2761 catttacagt gcgtgcggct ttacagacta ttcttccttc tacagaaact tcaagtcctt
     2821 ttatggactt tcccctagtg aatacaagga gttctacgac aaggggcctg ccaatgatta
     2881 aggaaaaaga gttcgcagac caaaccttca cgaactcttt cctctttatg atagaattct
     2941 cttcaagttc tccgctgcaa aattaaatgc ttccttgttg gacttaaaac tcacctctcg
     3001 cttaaactcg ccttctgtaa gacggtcaag accaacaaaa ctcataaggt gcggctcaag
     3061 agtcacatga acaacctctt tttttgcagc aaagtccttt acaatctcct ttatccttgc
     3121 ctgtccgtac cctggtactg tgattctgcc atccttcgtt ccatctttta tatggaagta
     3181 ctcgatgtaa ccgcagagca tcttgtacgc ttgtagcgga tcgtatcctg ctaatataaa
     3241 gttgcccatg tcaaatacac atctcaacct gccttcaaag ctttcaaaaa gttcgagaca
     3301 atcctcagga gactcgccgt agattccttc ctcgttttca tgacaaagag ttacaccata
     3361 cctgtccgcc aattcaagaa gtttctccac cctttcaaat gcagtctttc taaattcttc
     3421 tatcggatgt ctatttctat tgtaaaaact gaacatacga atgtatttac ttccaaaaat
     3481 atttgcactt tcacatatcc tttcaagttt taccaaatgt tcgttgaaat catccgtaat
     3541 atcaatcttc ccgataggtg agcctatgga aaaaacactg ataccgtttt cctttaaagt
     3601 ctcattcaac ctttttacag tttcaaggtc gatatctgca atgttttttc catcaacaaa
     3661 ccttatctct aagtaatcaa ttttattttg aataagccct tcaatctgct cctcaaggct
     3721 cactgcatat tcatctgcaa acgctccata aacaatattc attctttccc tcctataaga
     3781 attttgaata ttcaaccact tctccctgat gaataacacc ttttaatatt aacatcatct
     3841 tcaaatatta caatatcggc ataacaacca atttttatct cacccttgta ggaaagaccc
     3901 attattcgag caggattcct ggtaatcaac tttactgcgc tgcatttggg tataacagcg
     3961 tctttcacac atacccttac cagtctgtca cggttggcga tgctgcctgc aaacacagta
     4021 cgatcaggaa gttatgtaca ataatacaca gtgcgcctcc cacgcttgct gttttctcac
     4081 tagttcctgc acagcttaat gaatttgata ccaaacatat aatcatcagt ttttatcttg
     4141 taaatcagtt taattagctc tggcgaaaat gcctgccgtt tgcaattatc tctacatcaa
     4201 tatcatcgat aagatacgcc gtttctatta gtcctagctt tctgaatccc ttctccctgg
     4261 ttatggttga catgcattaa taaagatacg tggccagctt gtttccatta caatatgcct
     4321 gaatcatctc atcatatgtg cagtttgtat gcactgatga caaaaaatgt tgttctcacc
     4381 cattcttttt gagataatcg gaaccaagta attcctgtgc atagcttatt cttgccaata
     4441 tgtctccaaa ctggtttacc agataatcaa tctctttcct gttcgaattc tgttggacgg
     4501 agccgctgta aagatcatgt ttatgtacta tttctcaact aaaagctttt ctattttgta
     4561 ttatactttt atactcctgg gagaggatag atccaattgt gtatgagtct ttttctcatt
     4621 attgtcaatt tttcttctcc cctgtttcaa tagcttttca caaggcataa aataaagcat
     4681 gacagtataa aatgacagga gtgtaaaaga ttgatttatc ttctcctctt tacatggtgt
     4741 cttccccaga cgctattggc atttattata agggccttcg ttaaacctgt tgtgtcagag
     4801 aaacggggta atgtgaatgt atacaaggtt gattgggata caggttctct gtcaatgagc
     4861 aacaaggtat ttctgtgcaa aacccactgg gagaacgaga aggttttaat gcacgaaatt
     4921 ggccacttca agcagagcct tattctggga tggttctttc tcccgattat tggtttgcca
     4981 agtttaatgt gggcttggtg gggacatgat ttggcaaaca agtaccggga gaaaaaaggc
     5041 ctccacaaag tcgactacta ctccttctac actgagaaat gggcaaacaa actcggagga
     5101 gtggattaaa tcaagcaatg gtctgagctg gcagacttgt attccatttt ccacatcggt
     5161 ccccaaagca tcctattgcc ttttcattct catagattct gacaacctca caacaattgg
     5221 gacaacctgc gcactcaaag cttttagaca caaatgtact gtcagcaaga ccaaacccct
     5281 tgaagtttgt tcttcctgtc tttctcactg cattccttgc tattattgca gcgccgattg
     5341 ctcccatcat atcatagtaa tcaggtacaa ttacttcaaa acctaaagca tcctcaaaag
     5401 ctttctttat acccttgttt gctgcaacac cgccttgaaa aaacaccttg ggtaaaatct
     5461 ccttcccctt gcctatattg tttaaataat ttctgacaag agcctcgcaa agcccgccaa
     5521 taatgtctgc ttcatagtat ccaagctgtt gcttgtgtat catgtcagac tctgcgaata
     5581 ccgcacacct gccagctatt ctcaccggtg ttgttgcttt taacgcataa tctccaaact
     5641 cttctatggg tatatccagt ctttct
//
LOCUS       DAONVY010000083         5660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10536_length_5660_cov_3.884211, whole genome shotgun sequence.
ACCESSION   DAONVY010000083 DAONVY010000000
VERSION     DAONVY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10536_length_5660_cov_3.884211"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLI11_03420"
     CDS             <1..1020
                     /locus_tag="PLI11_03420"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPZ51944.1"
                     /translation="PKPPVLKSNVSKQTGESIKQEAPLREKVETAVTNQHESDVVISK
                     ADEASSVHKTSTSKKSYHGTHGKHGYKNEAFKALGVVLAVIVVIIGGIGLIFLASQRS
                     DAATVKTINNPNANISDLDSFKKYKNLEHLHLYNSGIQDINGIKDLTKLKVLRLTQNE
                     IKDISVLKNLKNLEELYLGDNQISDISALEGLTKLKVLHLGKNKIKDVTPLKNLVNLE
                     ELELGYNEIEDISPLANLTKLRILKLNNNKIENIEALSNMTEMRFLLLRENGDIKDLT
                     PLKGLHKVEWCSFNPPSLEIETVIMLKEANPDAIYYFYYKGDYIKAHEDFIYDPGPPP
                     DPGEG"
     gene            1178..2155
                     /locus_tag="PLI11_03425"
     CDS             1178..2155
                     /locus_tag="PLI11_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPZ51945.1"
                     /translation="MKKVQKKNSVSYILLLFALMVVMFAAVVVAHSLKGLDSDILGNS
                     PTITPITPTPSLEDETQTPQTTTDPDVTQTPTPVPTPTPTPTPIPIIEEIDESKLVGL
                     DTTVRSWSWAYPYKEVEKHGGVYRKTNDGKVLYLTFDNGYENGFTPRILDILKEKNVK
                     AIFFVTGSYIRKNQELVKRMVEEGHLVGNHTNTHPEKTPMPDLSVEKFVEELVVVERE
                     YEKIFGKGQRMFYYRPPKGLFSIRDLHIAAQMHYETIFWDLAYQDYGDPVGVEEAINR
                     VIGPIQEGKFVGNGAVILLHSTFEDNANALATIIDTLREMGYEFRRIDE"
     gene            2152..2847
                     /locus_tag="PLI11_03430"
     CDS             2152..2847
                     /locus_tag="PLI11_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51946.1"
                     /translation="MRVKRSFVIKVSIVFLVILMGYSVVDYFRESVEKMTSPEEHFPK
                     LHNAERYFFRVGYPEDWAAEAGANNFFLDKDTGLVLNLYPLIKSSDATPIPQETGQED
                     VQDPMVRDETAIISFFYRPIEKGEEMDLKQAMDIALAELMESGKNITEDNISSIMDYE
                     SEKTVFKKVSYTYMKNLFPVKGDMYVAVRQMCYYIIIYEAEHDVLAGSSYNKYKDVAG
                     EIIDDFRLSVFDY"
     gene            2859..3845
                     /locus_tag="PLI11_03435"
     CDS             2859..3845
                     /locus_tag="PLI11_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51947.1"
                     /translation="MKRLCLVVVILVVVSAAGCTKGNNIPKKSDVWHIEGLAYDGKII
                     AENEDFRVYDFVFMHFCTDAKNKDEALNKAHLYSVTYEIARLNTPLTEEEKEEILRLS
                     EDLAKDNAELYLNKGFENVLQYKLFALMDNTINKYKSSVFEKYDIDDKDLLDYYNQNL
                     KKYKSVALQVLYYSLKDENGNKLSWEVIEEKINKAESYLDEIKTSSDMDDLILNESDD
                     IGVIETGGKMTVSLEGYQNKDDELYEFSSNPDLKVGDMIVIVDEYGIYLVRCDGFEDY
                     DNSENVRDAVYNDYCTFKFLTEMETYLEDEKYYLKDIKEEIMDKITTQYQWD"
     gene            3858..4703
                     /locus_tag="PLI11_03440"
     CDS             3858..4703
                     /locus_tag="PLI11_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPZ51948.1"
                     /translation="MKGLLLSIILVEMLLTGCVRNIDKMTPSPKISPEVTDRADDTPG
                     ATESVTPTSTPLPTPSPTPISEPVTNVYQAVKRLVVAEKASHVFVVLQREKGPECDFY
                     AFKRQGNEFVEVFNTVGLVGKNGIATIEEKHEGDYRTPAGIYTFGMCFGIKDDNGISP
                     LGYTVVGEDDYWDSNPESDTYNQWIKKKDLPATYDKTKYEHLISKKGTYNYVAAINYN
                     VDPIIKGKGSAIFLHCTREGSMLYTGGCIAIPEDKMLIALSMIYSDTYIVIASSFDDL
                     MTHVR"
     gene            4793..>5660
                     /gene="dpsA"
                     /locus_tag="PLI11_03445"
     CDS             4793..>5660
                     /gene="dpsA"
                     /locus_tag="PLI11_03445"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HPZ51949.1"
                     /translation="MKNMKFTLIGGDLRNVKLASLLEERGNEVYTFGLEGGAGGKFFY
                     RCNSMTEAFARGDIIIGPIPFSQDGIKLNAPLHQADVELQDILDNIPKGRLLIAGKVP
                     ADFVKKVADKNVRIIDIMDRDDMAILNSIPTAEGAVQIAMEELPVTIHNCKVMVLGLG
                     KVGSTLAVLLKNIGAKVLVVARNSRDISRAAAFGLECGMYEELPLLIGEYKLIYNTVP
                     AMVLDKSILQFVAKDALVIDLASKPGGVDFEYAQYKGIKTIHALSLPGKVAPVTTAMY
                     METVIYNIIKEEY"
BASE COUNT         1888 a          881 c         1295 g         1596 t
ORIGIN      
        1 cccaaacctc ctgtattaaa gagcaatgtt tccaagcaga cgggtgaaag tataaaacag
       61 gaagctcctt tacgtgaaaa agttgaaacc gcggttacta atcagcatga atcggatgtt
      121 gtgatttcaa aagcagacga agcttcttct gtacataaga caagtacaag caagaaatca
      181 taccatggaa cgcatggtaa acatggatac aagaatgagg ccttcaaagc tttgggagta
      241 gtactggcgg ttatagttgt aattataggt ggcataggtt tgatttttct tgcttcacaa
      301 agaagcgatg cggcaacagt aaagacaatt aataacccca atgcgaacat atcggatttg
      361 gattcgttta agaaatacaa gaatctggag catttgcatc tgtataacag tggaatacag
      421 gacataaatg gaataaaaga tcttactaaa ctgaaggtac taagactgac acagaatgag
      481 attaaggata taagtgtgct aaaaaatctt aagaatctcg aagagttgta tcttggtgac
      541 aaccagatat cggacattag tgctttggaa gggttgacca agctgaaagt tctacatctt
      601 ggcaagaata aaataaaaga tgtgactcct ttgaagaatc tggttaatct ggaggaactg
      661 gaactcggat acaatgaaat tgaagatatc tctcctttgg ctaatttgac caagttgaga
      721 attcttaagc tgaataacaa caagattgaa aatattgagg ctttgagcaa catgactgag
      781 atgagattcc tgttgcttag agaaaatggt gacattaagg atttgactcc gttgaaggga
      841 ttacataaag tggaatggtg ctccttcaat ccaccaagtc ttgagattga gaccgtaata
      901 atgttaaagg aagcaaatcc tgatgcaatc tactatttct actacaaggg cgattatatt
      961 aaagctcatg aggatttcat atatgatcca ggaccaccac cagatcctgg tgaagggtaa
     1021 gacatggagc gaattttata aaaagtttag taaatgtata cgggtagttt agataactgc
     1081 ccgtttttcc attgataata tctctaattt atgctataat ttttgtgttt taatcataat
     1141 attagtgttc aaataataca taggagattt gtttatcatg aagaaagtac aaaagaaaaa
     1201 tagtgttagt tacattctac tattatttgc gctgatggtt gtaatgtttg cagctgttgt
     1261 tgtagcgcat tcgttaaagg ggttagatag cgacattctt gggaattcgc caacaataac
     1321 tccaataaca ccaacacctt cactggaaga tgagactcaa actccacaaa caactactga
     1381 tccggatgtt acgcaaacac caacaccggt gccaacacca acaccaactc cgacgccaat
     1441 tccaattatt gaagagatag atgaatcaaa gcttgttggg ctggatacaa cagtaaggtc
     1501 ttggtcctgg gcatatccat acaaagaagt tgaaaaacat gggggagtat acagaaagac
     1561 aaatgatggc aaagtattgt atcttacatt cgacaatgga tacgaaaatg gttttacgcc
     1621 tagaatactt gatattttaa aggagaagaa tgtaaaggca atattctttg ttacaggaag
     1681 ctacattaga aagaaccagg aacttgtaaa gagaatggtg gaagaaggac atttggttgg
     1741 taaccatacg aacacacatc ctgaaaaaac tccaatgcct gatttgtccg ttgagaagtt
     1801 tgttgaggaa ttggttgttg tagaaagaga atacgagaag atatttggaa aaggtcagcg
     1861 tatgttttac taccgtccgc caaaaggact ctttagcata agggatcttc atattgcagc
     1921 ccagatgcat tatgaaacaa ttttctggga tcttgcctat caagactatg gtgatccggt
     1981 cggtgtggaa gaagcaatca acagagtgat tggaccaata caagaaggca agtttgttgg
     2041 aaatggtgct gtaatattgt tgcattcaac atttgaagac aatgcaaatg ctcttgctac
     2101 catcattgat actttgagag aaatgggata tgaatttagg aggatagacg agtgagagta
     2161 aaaagaagct ttgttataaa agtttcgata gtgtttcttg tcatactgat gggatattct
     2221 gtagttgact actttaggga aagtgtggag aaaatgacat ctccggaaga gcattttccc
     2281 aagcttcata atgctgagag atattttttc agggtaggtt atcctgaaga ctgggctgct
     2341 gaagcaggtg ccaacaattt tttcttggac aaggatacag gacttgttct caatctatat
     2401 cccttgataa agtcgtcaga tgcaactccc attccccagg aaacaggaca ggaagatgta
     2461 caggatccca tggtcaggga tgaaacggcc atcatctcct ttttttacag gcctattgag
     2521 aaaggcgaag agatggattt gaaacaagca atggacattg ctctggcaga actgatggaa
     2581 tcgggcaaaa atataactga ggataatata agttcgatta tggattacga atcggaaaag
     2641 acggttttca agaaggtgtc ttacacatac atgaaaaatc ttttccctgt aaagggcgac
     2701 atgtatgtag ctgtcaggca aatgtgttat tatataatca tatatgaagc ggaacatgat
     2761 gtgcttgcag gcagttcata caacaagtac aaggatgtgg ccggtgagat tatagatgat
     2821 ttcaggcttt ccgtatttga ctattaaaga ggtaatctat gaaaaggctt tgtttggttg
     2881 tcgttattct cgttgtcgta agtgcggcag ggtgcactaa aggcaataat ataccgaaaa
     2941 aaagcgatgt atggcatatt gaaggattgg catacgatgg gaagataatt gcggaaaatg
     3001 aggactttag ggtatatgat tttgttttca tgcatttttg cactgatgcc aagaataagg
     3061 atgaagcact aaacaaagct catttatatt ctgttactta tgagattgca cgtttaaaca
     3121 ctcctcttac tgaggaagaa aaagaagaga tattgagatt aagcgaagac cttgcaaaag
     3181 ataatgcaga actgtatttg aacaagggtt ttgagaatgt tttgcagtac aaactctttg
     3241 ctctaatgga taatacgatt aacaagtaca aaagtagtgt gtttgaaaag tatgatattg
     3301 atgataaaga ccttttagat tactacaacc agaacttgaa gaagtacaaa agtgtggctc
     3361 ttcaggttct ttactattct ctaaaggatg aaaacggaaa caaactatct tgggaagtta
     3421 ttgaggaaaa gataaataaa gctgaatctt atttagacga aataaagaca tcaagcgata
     3481 tggatgattt aatcttgaat gagtcagatg acatcggtgt tattgaaaca ggcgggaaga
     3541 tgactgtatc tttggaagga taccagaaca aggatgatga attatatgag ttttcgtcca
     3601 atcctgattt gaaggtagga gacatgattg taattgttga cgaatacgga atttatcttg
     3661 tcagatgcga tggttttgaa gattatgata atagcgaaaa cgtaagggat gctgtgtaca
     3721 acgactattg tacttttaag ttcttaacgg aaatggaaac ttatttggaa gatgaaaagt
     3781 attatcttaa ggacataaaa gaagagataa tggataagat aacaacgcag tatcaatggg
     3841 attaaaggga gaattttatg aaaggattat tattatccat tattcttgtg gaaatgttgt
     3901 tgaccggttg tgttaggaac attgacaaaa tgactccatc accaaaaatt tcacccgagg
     3961 ttaccgacag ggctgatgac acacccgggg cgacagaatc tgtcacgcca acatctactc
     4021 cgttgccaac gccatcacca acccctattt ctgagcctgt tacaaatgta tatcaggcag
     4081 tgaaaaggct tgttgtggca gaaaaagcat ctcatgtatt tgttgttttg caaagagaaa
     4141 agggtcctga gtgtgatttt tatgccttta aaagacaagg caatgagttt gttgaagtgt
     4201 ttaacactgt tggtcttgta gggaagaatg gtattgctac aatagaggaa aaacatgaag
     4261 gcgactacag aactcctgcc ggaatatata catttggaat gtgttttgga ataaaggatg
     4321 acaatggcat atctccactt ggctatactg ttgtaggcga agatgattat tgggattcaa
     4381 atcctgagtc cgatacgtac aatcaatgga ttaagaagaa agatttgcct gcaacttatg
     4441 acaaaacgaa atatgagcat ttaatcagca agaagggaac gtacaactat gtagcagcga
     4501 taaactacaa tgtggatccc ataattaaag gaaaaggttc tgcaattttc ctccactgta
     4561 caagagaagg ttccatgctg tataccggag gttgtattgc cattcctgaa gataagatgc
     4621 ttatcgcact ttcaatgatt tatagcgata catatatcgt aatcgcaagt tcttttgatg
     4681 atttgatgac acatgttaga taattcaggc tctgcctgaa ttttttcttt tatacaccca
     4741 aatcattaat cccacatatt atatatcgga aaaagatttg aagagtgata cgatgaaaaa
     4801 tatgaagttt acattgatag gcggagacct gcgcaatgta aagttggctt cactattgga
     4861 ggaacgagga aacgaggtat acacttttgg attggaaggc ggagcaggag ggaaattttt
     4921 ctacagatgc aacagtatga ctgaagcttt cgcaagagga gacataatta ttggaccaat
     4981 tccattttca caggatggaa ttaaattgaa tgctcctttg catcaagcgg atgtggaact
     5041 tcaggatata ttggacaaca tacctaaagg caggttgttg attgcaggaa aagtacctgc
     5101 ggattttgtc aaaaaagtag ctgataagaa tgtgaggata attgatatta tggataggga
     5161 tgacatggca atccttaaca gcattcccac agctgaaggt gctgtgcaga ttgcgatgga
     5221 ggagctgcct gttacaatac ataactgcaa ggttatggtt cttggactag gaaaagttgg
     5281 atcgacgctg gctgttttgt tgaagaatat tggagcaaaa gtactggtgg tggcaaggaa
     5341 cagcagggac atttcaagag ctgccgcttt tggtcttgag tgcggaatgt atgaggagct
     5401 gcctttgctc attggcgagt acaaacttat ttacaacacc gttcctgcga tggtgcttga
     5461 caagagtatt ctgcagtttg tggcgaagga tgccttggta atagatttgg cttccaagcc
     5521 gggcggtgtt gattttgaat atgcacagta taaaggcatc aagacaatcc atgcattgtc
     5581 tttgccggga aaggttgcgc cagttactac tgccatgtac atggagacgg taatatataa
     5641 cataataaaa gaagaataca
//
LOCUS       DAONVY010000084         5659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10538_length_5659_cov_3.332620, whole genome shotgun sequence.
ACCESSION   DAONVY010000084 DAONVY010000000
VERSION     DAONVY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10538_length_5659_cov_3.332620"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLI11_03450"
     CDS             <1..687
                     /locus_tag="PLI11_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPZ51950.1"
                     /translation="EKKARVAYYLGQLEEFDDAIKSIIDYLEKKGEPTFVLMYSDHLP
                     NLFYDVEEMSQDDKFVTKFYTWNNVGLEKLQDRHLELYHLSTYLCDMIGLDGNLVNKF
                     HRVYNNDPEYFHYYKLLQYYLMQEDINKDEYENDDYEIGGIIPFYIDSVTYDEEKRTL
                     TVIGDGFTENTFLCINDKVYDAEYINQSLIVVRDFRKPLAEGDIISVQIIGEKYGALL
                     RESEVIVYRG"
     gene            810..2417
                     /locus_tag="PLI11_03455"
     CDS             810..2417
                     /locus_tag="PLI11_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPZ51951.1"
                     /translation="MKKSTIRILIIQIALILTISALSGCSNNVGSDDDLQPGSYVIKR
                     EKPDIKSEEPVEGTATTFKVNKVFSNNMVLQRDEYIRIYGTAEASDEGKIVTASFKGL
                     HGSATVENGEWIITLGGTLPASKDMGHTLTVSGGDGVSYEFNDVLVGDVFWVVGQSNI
                     QYNLSTMLAEQPDCGVELNKDDAIRVCINNPQSGKFNGSDATTVYNDCMLHTRNTWQK
                     AYNYAKSGSAIGYTFAKNIYDATNGEIPIGIITFECAGAALSAFMPPEVCEEKGIGLT
                     QSTVLGDSTTRWIWNQLMYPFRHMTISGLVWYQGESDCLNHLYPIYAENLAATFEFYR
                     NTINQNYHDFPIFVVEFPSIYTMPAGTIGDWAFLDLGNVRGAISKVALLCDNVFMAAG
                     SDVWKDKTYWNSLHPKCKFEQSKRLSDLALSVLYDKVDIEYNEGPVAVKVKYNKKEVT
                     ITYRSVGDGLKAEGDKIIGFQIYTKGMWMDAEDVTIVDKNKIVIKYDYNITGVRYNCL
                     AEDCYPEEVNLSNSSGIPAIAFYDILD"
     gene            complement(2447..3238)
                     /locus_tag="PLI11_03460"
     CDS             complement(2447..3238)
                     /locus_tag="PLI11_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51952.1"
                     /translation="MKKRILLIFVLFAFVMSLNLVVAEEEESEFEGPGLLYDFYYEWD
                     DSWNYVPATWSGSMCDIEYSDNALLINIHGKDPYIFLEVYEEIELEEYPWIKVRIKNE
                     SATSETFEMYVIRDNEQISETNNFHYNIGKEHEEFMEFIIDARVVKSEQWWTGALTGL
                     RFDAVRNAAEEGGETFYLEYLGFFKTQEDAENFEPVRDATPPPTKEPTDTPAQTPKPT
                     NTPASTPKQDNSDKGGTMTLGIILSVVIILAAVVVAVIIVRKSRK"
     gene            complement(3261..4121)
                     /locus_tag="PLI11_03465"
     CDS             complement(3261..4121)
                     /locus_tag="PLI11_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51953.1"
                     /translation="MKGKLSLLISLVFLLALILPTAVSSEELQPVVIDFYYEDDFYDY
                     YSTFNGCDVTWDNNAVHMISTQDPELGENGDPFVVFKLDYMFSCDDYPWMRIKLKNES
                     VAPVFEMHFSTTSTSIVAGTCTHVDISTEDEEFKEYIFSVPERNIASMHINELDHEET
                     FWTGEINELRLDGMWVAEPSGQVPYGSEMYIEYVAFFPTEEAANNFDIETFRAARTPR
                     PTATPKPDPTATPERTETKKTEAPKQTETNKNDTDGGNNSALIITAVVGVIVVVAVAA
                     AIIIIRKKRT"
     gene            complement(4249..>5659)
                     /locus_tag="PLI11_03470"
     CDS             complement(4249..>5659)
                     /locus_tag="PLI11_03470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HPZ51954.1"
                     /translation="DGSKLTLKETHRFSNDPVLLNSTFTWDFLRLFHEMKQGILKSAL
                     AGGKDISSIGVDTWGVDFGLLTKDGELLGNPVHYRDSRTDGMIEEACKVIPQEEIYAE
                     TGIAFQKFNTLYQLLALNKNNSVMLENADTMLFVPDLFNFYLTGQKVCEYTITSTSQL
                     YNPVKGQWAYEMLEKLGIRTDILPEVVPAGTKLGGLTKELCDELGVASIPVIAVAAHD
                     TASAVVSVPFTEEGGAYLSSGTWSLLGVELDKPIINENALKLNYTNEGGINNNVRFLK
                     NIMGLWINQECKRHWEKIGEHISFDELDEAALNAQPFKAIIDVDDSVFFEPGNMPTKV
                     QEFCARTNQEVPQTKGEIVRCVMESLALKYRYVIEGLEEIIGKKLPKLHIVGGGCKNT
                     ILSQFTANAINRPVVAGPIEATSIGNLAVQLISLGEVKNLQEARQLIANSFPQEVYMP
                     ENPAAWDDAYEKFLKIQKS"
BASE COUNT         1713 a         1176 c         1110 g         1660 t
ORIGIN      
        1 gaaaagaaag ctagagtagc atattacctt ggccaattgg aagagtttga tgacgctata
       61 aaaagtataa tagattatct ggaaaaaaaa ggcgaaccga cctttgtact aatgtattcc
      121 gatcatctgc caaatctctt ctatgacgtg gaggaaatgt cgcaggatga caagttcgtt
      181 accaagttct acacatggaa caatgtaggc ttggaaaaat tgcaagacag acatctggag
      241 ctgtaccact tgtctaccta tctgtgtgac atgataggac ttgatggaaa cctcgttaac
      301 aagttccaca gggtgtacaa taacgaccct gagtacttcc actattacaa actgcttcag
      361 tactatttga tgcaggaaga cataaacaag gatgaatacg aaaatgatga ttatgaaata
      421 ggtggaataa taccgtttta tatagacagt gtcacatatg atgaagaaaa gagaacacta
      481 acagtaattg gtgatggttt taccgaaaac acatttttat gcatcaacga taaagtgtat
      541 gatgcagagt atataaacca gtcgctgata gttgtcaggg atttcagaaa gcctcttgcc
      601 gaaggagaca tcatatccgt tcaaataatt ggcgagaaat atggtgctct gctaagagaa
      661 agtgaagtaa tagtatatag aggatgacaa tatattggaa atacatggag gtactgaaac
      721 ttaatactac agtatctcca tttttttagt tgcaaatttc agtaattagt gtaaaatata
      781 ataaacaaat tagaaagaag ggaaactatt tgaagaaaag cacaataaga atcttaataa
      841 ttcagattgc actaatatta acaatcagcg ctttgtcagg ctgcagcaac aatgtcggtt
      901 ctgacgatga cttgcaacca ggtagttatg taataaagcg tgaaaaacct gatatcaaat
      961 ccgaagagcc ggttgaagga accgctacga ctttcaaggt aaacaaggtg ttcagcaata
     1021 atatggttct tcaaagggac gagtatatca gaatatatgg aacagctgaa gcaagtgatg
     1081 aaggcaaaat tgtaaccgct tcctttaaag gtttgcatgg gagtgcaact gtggaaaacg
     1141 gcgaatggat aattacattg ggaggtaccc ttcccgcttc gaaagatatg gggcacacac
     1201 tgactgtttc aggtggagac ggagtaagtt acgagttcaa tgatgttctg gttggagatg
     1261 tgttttgggt tgtgggacag tcaaatatcc agtacaatct atcaaccatg ctggccgaac
     1321 aacccgattg cggcgttgaa ctgaacaagg atgatgcaat cagggtgtgc ataaacaacc
     1381 cacaatccgg caaattcaat ggttcggatg caactacggt ttataatgac tgtatgcttc
     1441 acacaaggaa cacatggcaa aaagcataca attatgcaaa atcaggttca gcaataggct
     1501 atacttttgc aaagaacata tacgatgcaa ccaatggtga aatccctatc ggtattatta
     1561 cattcgaatg cgcaggagct gcattgtctg cattcatgcc acctgaagtc tgcgaagaga
     1621 aaggcatagg tcttacacag agtacagttc ttggcgattc aacaacccgc tggatatgga
     1681 accagcttat gtatccattc cgccacatga caatcagcgg tcttgtatgg tatcagggag
     1741 aaagtgactg cctgaatcat ctatacccca tttacgcaga gaatctggct gctactttcg
     1801 agttttaccg taataccata aaccaaaact atcacgactt ccctatcttt gtagtggagt
     1861 tcccttcaat ttacacaatg cctgctggaa caataggaga ttgggcattt cttgatttgg
     1921 gcaatgttcg tggagcaata agcaaggtgg cgcttctttg tgacaatgtt ttcatggcag
     1981 caggttccga tgtctggaag gacaaaacat actggaacag cttgcatcca aagtgcaagt
     2041 ttgagcaatc aaaaagatta tccgaccttg ccttaagtgt attgtatgac aaagttgaca
     2101 ttgaatacaa tgaaggtcct gtagcggtta aagttaaata taacaaaaaa gaagttacta
     2161 ttacatatag aagtgttgga gatggtctta aggccgaagg cgacaaaatt ataggttttc
     2221 aaatctatac aaaaggtatg tggatggacg ctgaagatgt gactatagtt gacaagaaca
     2281 agatagtgat taaatacgac tataacataa ccggcgtaag atacaactgc cttgcagaag
     2341 actgctatcc tgaagaggta aatctatcaa acagtagtgg cataccagca attgctttct
     2401 atgacatatt ggattaagat aagaggctgc aaatcgcagc ctccttttac tttcttgact
     2461 ttctaacaat aataacagct acaacaacag cagctaatat tatgaccacc gacagtatta
     2521 tacccaaagt catggttcca cccttgtcag aattgtcctg cttgggagta gatgccggag
     2581 tatttgttgg tttgggagtt tgtgccggtg tatctgtagg ttcttttgtt ggcggcggag
     2641 ttgcatccct taccggctca aaattctccg catcttcctg cgtcttgaag aagccaaggt
     2701 actctagata aaaggtctca ccgccttctt cagcggcatt cctgacagcg tcaaatctca
     2761 gccctgttaa agctcctgtc caccactgtt cactcttgac aactctggca tcaatgataa
     2821 attccataaa ttcttcatgt tccttgccaa tgttataatg aaaattgttt gtttcggata
     2881 tctgctcatt gtccctaata acatacattt caaaggtttc agaagttgca ctttcattct
     2941 taatcctaac ctttatccaa ggatactctt ccaattcaat ttcctcgtaa acctcaagga
     3001 agatatatgg atcttttcca tggatattaa taagcaaggc attgtcgcta tactcgatat
     3061 cgcacatcga gccgctccat gtcgcgggaa catagttcca tgagtcgtcc cactcataat
     3121 aaaaatcata caacaaacca gggccttcaa attcactttc ttcctcctcg gcaactacaa
     3181 gattgagcga cattacaaac gcaaacaaaa caaagatcaa taaaattcgt tttttcataa
     3241 ctaaattacc cctagttcct ttacgttctt ttttttctaa taattattat cgctgctgca
     3301 acagcaacta cgacaataac gccaactaca gcagtaataa ttaatgccga attgttgccg
     3361 ccatcagtat cgttcttatt ggtctcggtt tgcttaggag cttcagtttt ctttgtttct
     3421 gttctttcag gcgttgctgt tggatctggt tttggtgttg cagttggtct tggtgttcta
     3481 gccgcacgaa aagtctcaat atcaaagttg ttggcagctt cttctgttgg gaaaaatgcc
     3541 acgtactcta tgtacatctc cgagccatag ggaacttgtc cgctgggctc cgcaacccac
     3601 attccgtcca atctcagttc attaatctca ccagtccaaa aagtttcctc gtgatccagc
     3661 tcatttatat gcattgaagc aatatttctt tcaggaacac tgaagatata ttccttgaat
     3721 tcctcgtcct ctgtgctgat atccacatgc gtacaagttc cagcaactat ggatgtggag
     3781 gtcgttgaaa agtgcatctc aaaaacaggc gctacacttt cattcttcag ctttattctc
     3841 atccaagggt aatcatcaca actaaacata tagtcaagtt tgaatacgac aaatgggtct
     3901 ccgttttcac ccaactccgg gtcctgggta gaaatcatat gtactgcatt attatcccaa
     3961 gttacgtcgc aaccattaaa agtagagtag taatcataga aatcatcttc gtaataaaag
     4021 tcaattacaa caggctgtaa ttcctctgat gaaaccgctg tcggtaaaat cagagcaagc
     4081 aaaaaaacaa gtgatattaa caaacttaat ttacccttca tattctacat acctcctaat
     4141 tgttttttta tatcataaca cgtttgttca agaaattcaa tatttattaa aacaaaaaac
     4201 acctccgata tttctatcag aggcctttaa taaaacaaat attgtctgtt agcttttttg
     4261 tatcttcaaa aacttttcat acgcatcatc ccaggctgca ggattctcag gcatgtaaac
     4321 ttcctgtggg aatgagtttg caataagctg tcttgcttcc tgaaggttct ttacctcacc
     4381 aagagagata agttgaacag ccaggtttcc tatggaggta gcttctatag gtccggctac
     4441 aacaggtctg tttatggcat tggctgtaaa ctggctcaaa atagtgttct tgcagcctcc
     4501 acctacaata tggagtttgg gaagtttctt accgataatc tcctcaagac cttcaataac
     4561 atatctgtac ttgagtgcta ggctttccat cacgcagcgc acaatttcgc cttttgtctg
     4621 aggtacttcc tgatttgtcc ttgcgcagaa ttcctgtact ttggttggca tattgccagg
     4681 ttcgaagaat acagaatcat caacgtcgat gatcgccttg aatggctgtg cgttgagagc
     4741 agcttcatca agctcatcaa atgaaatatg ttctccaatt ttctcccaat gacgtttgca
     4801 ttcctgatta atccaaagac ccattatgtt cttgaggaat cttacattat tgttaatacc
     4861 gccttcattg gtgtaattaa gtttgagtgc attctcattg attataggct tgtcaagttc
     4921 cacaccaagc aatgaccatg tgccgctact tagataagct cctccctcct ccgtgaatgg
     4981 gactgataca acagcacttg ccgtatcatg agctgcaaca gctataacag gtatcgatgc
     5041 aacaccgagt tcatcacata actcttttgt aagtccgccc agctttgttc cggcaggtac
     5101 aacctctgga agtatgtcag ttctgatacc cagcttttcg agcatttcat aagcccactg
     5161 tcccttgaca ggattgtaca attgtgacgt gctggttatt gtgtattcac acaccttttg
     5221 tcctgtcaga tagaaattga acaagtcagg cacaaaaagc attgtatcgg cattttcaag
     5281 catcacactg ttgtttttat tcaacgcaag cagttgatat agagtattga acttctgaaa
     5341 tgcaattcct gtttcagcat agatttcctc ctgaggaatc accttgcagg cttcttcaat
     5401 cattccatcg gttctggagt ctctgtaatg tacgggattg ccaagaagct cgccatcttt
     5461 tgtcaataga ccaaagtcga caccccaggt atcaacgcct atggagctta tatcttttcc
     5521 tccagccaaa gctgatttta gaatcccttg tttcatttcg tgaaacaatc taagaaaatc
     5581 ccatgtaaat gtgctgttta gcaaaacagg atcatttgaa aacctgtgcg tctctttcaa
     5641 tgtgagcttg ctgccatca
//
LOCUS       DAONVY010000085         5655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10543_length_5655_cov_3.172500, whole genome shotgun sequence.
ACCESSION   DAONVY010000085 DAONVY010000000
VERSION     DAONVY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10543_length_5655_cov_3.172500"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..2143
                     /gene="recG"
                     /locus_tag="PLI11_03475"
     CDS             92..2143
                     /gene="recG"
                     /locus_tag="PLI11_03475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPZ51955.1"
                     /translation="MASTPLEIFLNKNVMDLKGISVKKAASLRKLGINTINDIIMNIP
                     RSYEDRRNIKKIADVMVGETATVIGKVVSFDHVFIRRNLTITTVLIQDGTSSLFVKFY
                     NQNYLEKVLVKGRAYLFYGTVQAGYEGPVMEMPTYARLENKDVFAVIHPIYSLTKGVS
                     QYMIHKLVKEVLHAVKHFKDTMPQYIVDKYHLMSKDEALRNIHFPKNEELRTNARFRL
                     AFEELLELQILLHSIKEDTISNATGIQFIKNNTTKKFIENLPFKLTNAQLRVWSEIEK
                     DMASDKIMNRLVIGDVGSGKTIVAVLAMLNAIVNGYQALYMAPTEILAEQHMKTVSSF
                     LNPLGIKTALLTGSLPAAKKRQLLEEIEKGEIQCVVGTHALIQRNVTYDNVGIVITDE
                     QHRFGVRQRAQLAAKGKTPDVLVMTATPIPRTLALILYGDMDLSTIDELPAGRIPIKT
                     YAVDSTMRDRILAWTKKLVKQNQQIYIVHPAVEESENMNLLSATENYKKLSTTVFKDI
                     PTGLVHGKMSAKAKEEVMRDFVAGKIKILFSTTVIEVGVDVPNATLMIIENAERFGLS
                     QLHQLRGRVGRNNIQSYCVMITDSKTDLAKERMTIMVKTNDGFKISEKDLELRGPGEL
                     FGTQQHGIPEFKVANLYEDLDILGIAQDAAFTIMKNRNDEQTAWYIKSVIKKAEGMFN
                     L"
     gene            2249..2449
                     /locus_tag="PLI11_03480"
     CDS             2249..2449
                     /locus_tag="PLI11_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HPZ51956.1"
                     /translation="MAKCSICDKGVTFGRRISITRSQVSRRAKRKWKPNIRRVKIVDN
                     GTTRYAHVCASCLKSGRVTRAV"
     gene            2708..3370
                     /locus_tag="PLI11_03485"
     CDS             2708..3370
                     /locus_tag="PLI11_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51957.1"
                     /translation="MFRSLTVKAFFSKKPMKRIIVTFSLVIAIWLLLIAFFIYIENHK
                     AYVISIDDLSVSFPTKIYITEPDSIEAAIRFKEYKSSNNEFWVRFPQAFQLREELFEG
                     RDITYHLNINGNDGIHGYVQIWQLDTTMQDFLNNARKYKSQSVYDFEQKEIQLGNNIK
                     GYKWCYSVKNQDRDIVARQSFISKPGSTKMYVITLYIQKKMYSDDFEKLYDDILYSVR
                     LR"
     gene            complement(3349..3978)
                     /gene="nth"
                     /locus_tag="PLI11_03490"
     CDS             complement(3349..3978)
                     /gene="nth"
                     /locus_tag="PLI11_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPZ51958.1"
                     /translation="MKDIKEIMELLEEAYPEAGCTLRYSDPLQLLIATILAAQCTDAR
                     VNLVTPTLFKRYPDCNAFAEADAHELEEYIRSTGFYRNKARNIIECCKRLKDVYNCVV
                     PDNMEDLLTLAGVGRKTANIILGDVYNKPAVVVDTHAKRLSNRIGLSKHKDPTKIEMD
                     LAKILPPDRSARFCHQLVYHGRAVCNARNPKCEICVIKEHCDYYRNLTE"
     gene            complement(3975..5384)
                     /locus_tag="PLI11_03495"
     CDS             complement(3975..5384)
                     /locus_tag="PLI11_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPZ51959.1"
                     /translation="MNMYEDDNMNEEVTEDVVVENSDSENVVEIEKEEEKDTPNTEYY
                     IENIRKLKQELKEAKRKEKEEKRRQREQIASTYDSSKKGVSIATVVVIAFIFAVIGFM
                     ASVVFSIFGNTLIESLTGRNMNKKNGDTIIIDNGPPINLEVDNIDSISTAVYAKAARS
                     VVGIQVVKTSGVFWDIQVQVIGEGSGVIYSEDGYIITNAHVIEKALSPETRDVMPNYE
                     VRVYLKTDLSEFFVANIIGYDTVTDLAVLRIDVTGLAPVEFADSNEVMVGDIAIAIGS
                     PGGLEFMNSVSKGIISGVNRNIVMGSGSSAYDLIQTDAAINPGNSGGALLNSEGKLIG
                     ISAIKIVSTDYEGMGFAIASNTITDIVEKLIKDRIVIRPALGVTVNTNYNSTIARQYG
                     YPNGALVYQVLRGSAADKAGMRENDIIVEFAGNKVNNFYDLRKELLKFAPGEEVVVKV
                     FRPSSEKEIELKVVLDEAQ"
     gene            complement(5465..>5655)
                     /locus_tag="PLI11_03500"
     CDS             complement(5465..>5655)
                     /locus_tag="PLI11_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPZ51960.1"
                     /translation="MIFERFFKSDKSRSEDKKGTGLGLSIVKKILIAHDQDIKVDSSL
                     GKGSKFTFTVARPPKEHS"
BASE COUNT         1747 a         1172 c         1088 g         1648 t
ORIGIN      
        1 atcattacaa tagactatat ttatttgaaa aaagatattt tatgtaatat actagaaaaa
       61 gtcagtatag aaaaatgtgg ggatgaatgg aatggcaagc actcctttgg aaatatttct
      121 aaacaaaaat gtaatggatt taaaggggat aagcgttaag aaggcagcaa gcctgagaaa
      181 actgggcatc aatacaatta acgacataat catgaatatt ccccgttcct acgaggatag
      241 gagaaatatt aaaaagattg ctgatgtcat ggtgggcgaa acagcaaccg taattggaaa
      301 ggttgtatct tttgaccatg tctttatccg cagaaacctt accatcacca ccgttctcat
      361 acaggacggc acatcaagtc tatttgtaaa attctacaat cagaactatc tggaaaaggt
      421 gcttgtaaaa ggaagggctt atcttttcta tggaactgtt caggcaggat atgaaggtcc
      481 agtaatggaa atgcctacat atgcacgtct tgaaaacaag gacgtgtttg ctgtaataca
      541 tccgatatac agtctgacaa aaggcgtatc ccagtatatg atccacaagc tggtcaagga
      601 agtccttcac gctgtgaaac actttaaaga cacaatgccc caatacattg tggacaagta
      661 tcacctaatg tcaaaagacg aagccctaag gaacatacat tttccgaaaa atgaagagtt
      721 aagaaccaat gcaagattca ggctggcatt tgaagaactt ttggaactgc aaatacttct
      781 gcattccata aaggaagaca caataagcaa tgcaacaggc attcagttta taaagaacaa
      841 tactacaaaa aaatttatag aaaaccttcc tttcaaacta accaatgccc agcttagagt
      901 ctggtccgaa attgaaaagg acatggcatc ggataaaata atgaacagac tggttatcgg
      961 agacgtaggc tccggcaaga ccattgttgc tgttcttgcc atgcttaatg ccattgtaaa
     1021 cggataccag gcactttaca tggcacctac cgagattctt gcagaacagc atatgaagac
     1081 agtatcttct tttcttaatc ccttgggaat caaaacagcc ctgcttacag gctcattgcc
     1141 tgcagcaaaa aaaagacagc tgcttgagga aattgaaaaa ggagaaattc agtgcgttgt
     1201 aggtactcat gcccttatac aaagaaatgt gacttacgac aatgtaggaa tagttattac
     1261 tgacgaacag cacaggtttg gagtaaggca aagggcacaa ttggcagcaa aggggaaaac
     1321 tccagatgta ctcgtcatga ccgcaacacc aataccaaga actcttgctt taatcctcta
     1381 cggagatatg gacctgtcca caattgacga actgcctgca ggcagaatac ccataaaaac
     1441 atatgctgta gattccacta tgcgggatag aatacttgca tggactaaaa agctggtaaa
     1501 gcaaaaccag cagatatata ttgtacaccc agccgttgaa gaatctgaaa acatgaatct
     1561 tctttctgca acggagaact acaaaaagct atccaccacc gtcttcaagg acataccaac
     1621 aggacttgta cacggaaaaa tgtctgccaa ggcaaaggaa gaagtcatga gggattttgt
     1681 tgcaggcaaa atcaagatct tgttttcaac aacggtaatt gaagttggag tggatgtgcc
     1741 aaatgcaacc ctaatgataa tagagaatgc agaaagattt gggctgtcac agcttcacca
     1801 gctgagagga agagttggca gaaacaacat acagtcctac tgtgtgatga taactgacag
     1861 caagacggac ctggcaaagg aaagaatgac cataatggtg aaaacaaatg acggatttaa
     1921 gatatcggaa aaggatctgg aactgagagg tcctggtgaa cttttcggta cccaacagca
     1981 tggaataccc gaattcaagg tggcaaatct gtatgaggat ttggatatat tgggtattgc
     2041 ccaggacgca gcctttacca taatgaaaaa cagaaatgac gagcagaccg catggtatat
     2101 aaaatccgtc ataaagaagg cagaaggcat gtttaattta taatttttga acaaattatg
     2161 gaatttttaa tttggtattg attttttaat acatagtatg atataatgag tgttgctgtt
     2221 taaaaagtaa acaaggaggt ggatttaaat ggcaaaatgc agtatctgcg ataaaggcgt
     2281 tacattcggt cgtcgaatca gcataacacg ttcacaggtc agcagacgtg cgaaaagaaa
     2341 atggaagcca aatattagaa gagtaaagat tgttgataat ggtacaacaa gatatgccca
     2401 tgtttgcgca tcttgcttga aatcgggcag agtaacaaga gccgtttaat ctacctgtta
     2461 tcaatagaag cgtggtaaaa aaatttccct ctgtttggag agggattttt tttagccttc
     2521 tattcatgtg tcctcttcat ccggaaaaga aacctaataa ctggtgcaac tacttttgga
     2581 gctttaacaa ctaatatctt catagagatc ccttcttcat tgttctcttg ctattaatat
     2641 attcaaaaat gaaaaaaagt gtgaaacctt caggttttga agcatataca ttaccgaggt
     2701 gatatatatg tttcgaagct tgacggtaaa ggcttttttt tcaaaaaagc ctatgaaaag
     2761 aataattgta accttcagcc ttgtaattgc catatggctt ttgttgattg cattcttcat
     2821 ttatatcgag aatcataaag cctatgtcat ttcgatagac gacctttccg tttcctttcc
     2881 gacaaaaata tatattacgg aaccagacag tattgaagca gccatcaggt tcaaagaata
     2941 caaaagcagc aacaatgagt tctgggtcag gtttccgcaa gcttttcaat taagggaaga
     3001 gttgttcgaa ggcagggaca taacgtatca ccttaatata aatggcaatg acggtataca
     3061 cggttatgtt cagatatggc aacttgacac gaccatgcag gactttctaa acaacgctag
     3121 aaagtacaag tcccaatccg tatatgattt tgagcagaag gaaatccagc tggggaataa
     3181 tattaaagga tacaaatggt gttattcagt aaaaaaccag gatagagaca tagtggcccg
     3241 gcaatctttc atctccaaac ccggttctac caaaatgtat gttataacac tttacataca
     3301 aaaaaagatg tattcagacg actttgaaaa actctatgac gatatactct attcagtaag
     3361 attgcgataa tagtcgcagt gttcttttat gacacatatt tcgcatttgg ggtttctggc
     3421 attacataca gcccttccgt ggtagacaag ctggtggcag aatcttgcgc ttctatcagg
     3481 gggaagtatt ttggcaaggt ccatctcaat ttttgtaggg tccttgtgtt ttgaaagccc
     3541 tattctgttg gaaagccttt ttgcatgcgt gtccacaaca accgccggct tgttgtacac
     3601 atcccccaga ataatatttg cagtcttcct ccccacccct gcaagtgtca gcaaatcctc
     3661 catgttgtcg ggaacaacac agttatatac atctttcagt cttttacagc attcaataat
     3721 atttcttgcc ttgttcctgt aaaaacccgt ggaacgtatg tactcttcca attcatgggc
     3781 atccgcctct gcaaacgcat tgcagtccgg gtatctttta aacagcgttg gtgtcaccag
     3841 attcaccctt gcatccgtgc actgcgctgc gagaatcgta gctatcaaaa gctgaagcgg
     3901 gtcgctgtac ctaagagtac agcccgcttc aggataagct tcttcaagaa gttccattat
     3961 ctcttttata tctttcactg tgcctcatcc aatactactt ttaattctat ttccttctcc
     4021 gagcttggtc tgaacacttt tacaaccact tcctcgcctg gagcaaactt caaaagttcc
     4081 tttcttagat cgtagaagtt gtttacttta ttacctgcaa attctacaat tatgtcattc
     4141 tccctcatgc cagccttgtc agcagcagag cccctcaaca cctgatacac aagagcaccg
     4201 ttcggatatc catactgcct tgctattgtg ctgttgtagt ttgtgtttac agttacacca
     4261 agagctggcc ttatgacaat tctgtctttt atgagttttt caacaatatc tgttattgta
     4321 tttgatgcaa tggcaaatcc catgccttca tagtcggtgg aaactatctt tatagccgat
     4381 atgcctatca gtttgccttc gctgtttagc agtgcaccac cgctgtttcc agggttgatt
     4441 gcagcatcag tttgaatcaa atcgtaagcg gaagaaccac tgcccataac gatatttctg
     4501 ttaacaccgc tgattatacc tttacttacg gagttcatga attcaagtcc acctgggctt
     4561 cctattgcta ttgcaatatc ccctaccata acctcgttgg agtctgcaaa ctccactgga
     4621 gcaagccctg ttacatcaat tcttagaact gccagatcag taacagtatc gtaacctatg
     4681 atgtttgcaa caaaaaattc ggataaatca gtcttcaggt acaccctcac ttcgtaattc
     4741 ggcattacgt ctcttgtctc tggactgagg gctttttcaa taacgtgggc attcgttata
     4801 atatagccgt cttcggaata aatgacaccc gagccctcac caataacctg aacctgtata
     4861 tcccagaaca caccggaagt ctttacaacc tgtatgccta ccaccgacct ggcggctttg
     4921 gcgtaaaccg cggttgaaat ggagtcaata ttgtctacct caaggttaat tggcggacca
     4981 ttgtcaataa ttatggtgtc gccattcttt ttgttcatgt ttctacctgt cagcgactca
     5041 atcagtgtat tgccaaagat actgaacacg acagaagcca tgaatcctat tactgcgaat
     5101 atgaaggcta tgacaacaac ggttgcaatg gatactcctt ttttggatga gtcataagta
     5161 cttgcaatct gctctctttg tcttcttttt tcttcctttt cttttctctt tgcttcctta
     5221 agctcctgct taagcttcct aatattctca atataatatt ccgtattagg agtgtccttt
     5281 tcttcttcct tttctatttc aaccacattt tcgctgtcgc tattttcaac aaccacatct
     5341 tcagtaactt cttcgttcat gttgtcgtcc tcatacatgt tcataatgtt tcctcctttt
     5401 tcattttttt cattgtaata attccatttt accatatagt gaaccaacaa tgaaaaattg
     5461 tgtcttaaga gtgttctttt ggtggtcttg ccactgtaaa tgtaaacttg gaaccttttc
     5521 caagactgct gtcaacctta atatcctgat catgtgcaat aaggattttc ttaactatgg
     5581 acaaaccaag acccgtgcct ttcttgtcct cacttctgga tttatcggat ttgaaaaatc
     5641 tttcaaaaat cattg
//
LOCUS       DAONVY010000086         5638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10578_length_5638_cov_5.392800, whole genome shotgun sequence.
ACCESSION   DAONVY010000086 DAONVY010000000
VERSION     DAONVY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10578_length_5638_cov_5.392800"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="PLI11_03505"
     CDS             <1..2008
                     /locus_tag="PLI11_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51961.1"
                     /translation="SGFDGNVQLGKTASDKKNIALYFNSVDMAGALMAKIKYVHTPTN
                     PFAKLSKDADSDRGHKVKPGTFAATDGLGRTVPGYEEVGDVREGKYVGMFFWTWHASF
                     SGTVPRNVTEILKEHPDIIHDYNAKQWGGPNDSYFWNEPIYGYYNGKDPWVYRKQAEL
                     LADAGVDVIIFDNSNNVATFIDGVKVLLETFAQARADGVNTPQISFLMNFFEADWSST
                     VIQLKELYKEIYKPGKYKELWFYWKGKPLMMAYPDKLNPNDALEAEILEFFTYRPAQP
                     SYTIGQIRPNQWGWCSVYPQQVYKDENGNPEQITVSVAQNHNDKRGLTAMNGERVFGR
                     HWINAKKDYDTRENAILYGANFEEQFNYALEVDPEFIFITGWNEWRAGRYREWCGVQN
                     AFPDQFNDAYSRDIEPSKGDLKDHYYYQLVSFIRKYKGVEKPESASAGKTIDIYSDQD
                     MWEDVKPYFAAYGGNTFHRNSPGYVGYHYENTTGRNDITGAKVTFDRDYVYFMVETKE
                     NLTPHTDKAWMRLFIDVEGQNGPDWETFEYVVNRVNPGEKAVLEKSTGGWNWEKVGDV
                     DYAVKDNRLQIKVPKSLLGIVGEKFVINFKWSDNMQNEGDIMDFYVNGDVAPGGRFKY
                     QYASYELYKESTFSLGKVITIVGAVLFVGLAVLSGILVVKKRKK"
     gene            2024..2728
                     /locus_tag="PLI11_03510"
     CDS             2024..2728
                     /locus_tag="PLI11_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51962.1"
                     /translation="MKKAIILVFLISLFVFSGCADKNAGQESTPTPEATHTPDPTSTP
                     TPTQEPTPKPTPMPTVEPGTGKYAGEVDETNGVKADFYEGGWDGEQAHRVNGSLCIQF
                     FATTTFDKVGVCMPTWTQKQGHAATISLYAWQGSFDATLMKDPIATKEVADWADGVEV
                     LLELDEEMPDGEYLYEIYNDNTNNVGIWYKPGTVDYVRTYYNNEIWDTGTPRLVVYYT
                     KTPEHLHGPIQDSGLE"
     gene            2857..3105
                     /locus_tag="PLI11_03515"
     CDS             2857..3105
                     /locus_tag="PLI11_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51963.1"
                     /translation="MGEKFVINFKWSDNMQNEGDIMDFYVNGDVAPGGRFKYQYASYE
                     LYKESTFSLGKVITIVGAVLFVGLAVLSGILVVKKRKK"
     gene            3121..3825
                     /locus_tag="PLI11_03520"
     CDS             3121..3825
                     /locus_tag="PLI11_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51964.1"
                     /translation="MKKAIILVFLISLFVFSGCADKNAGQESTPTPEATHTPDPTSTP
                     TPTQEPTPKPTPMPTVEPGTGKYAGEVDETNGVKADFYEGGWDGEQAHRVNGSLCIQF
                     FATTTFDKVGVCMPTWTQKQGHAATISLYAWQGSFDATLMKDPIATKEVADWADGVEV
                     LLELDEEMPDGEYLYEIYNDNTNNVGIWYKPGTVDYVRTYYNNEIWDTGTPRLVVYYT
                     KTPEHLHGPIQDSGLE"
     gene            3844..4938
                     /locus_tag="PLI11_03525"
     CDS             3844..4938
                     /locus_tag="PLI11_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ51965.1"
                     /translation="MYGMDEFFESVKELNEEELFYKEYYRIKKESPEKLGEYLEMLDR
                     EMMQEKGLWLPEIRQTSASYFTEYFVEDIRKNVEISIHNRYTPLFLHKHAFFEMVYVY
                     SGQCENTIDGNKIVMNKGDICIIAPDVSHTMGVFDDDSIVFNIIIRRSTFREIFEGIF
                     IDDTALSLFFNRILYSNTTNSHILFVSEDYESLHSIIKFLIWEGSKKEKYYKPAMENL
                     LRTVFCLMLRRQECMVVSSSLITEDPEILHILRYIQDNYKTVNLNDLSDKFKYSPDYM
                     GKLIKKNTGTTFIKILRDIKFKKACNMLLNTNLNVIDICYEVGYESVEYFTRSFKKLC
                     GMTPTEYRKQASNLKNIPPSQRSIELNTIT"
     gene            complement(4935..>5638)
                     /locus_tag="PLI11_03530"
     CDS             complement(4935..>5638)
                     /locus_tag="PLI11_03530"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPZ51966.1"
                     /translation="PVTEADRASNSIISNYLMEKYPEYAILAEETYDKKTIEERVLNP
                     YCLIIDPLDGTKEFIKKNDEFSINIALAYKKRAVCGMIYIPVFNKVYYAARGHGAYKS
                     TSKEFFDNIFDDKRRISVSGRTERLICMHSKSSFENETKKLLADNMHKIEKLVSKGSA
                     IKMCTIAEGKADVYYRYGHTMEWDTAAGQIIIEEAGGIMRQLNMNQTELLYNRYDSCN
                     RNGFYVINNINSRLV"
BASE COUNT         1739 a         1003 c         1333 g         1563 t
ORIGIN      
        1 aagtggtttt gacggcaatg tgcagcttgg caaaaccgca tcggacaaaa aaaatattgc
       61 cctgtatttc aacagtgttg atatggcagg agccttgatg gcaaagataa aatacgtaca
      121 tactccaaca aacccttttg cgaagttgtc taaagacgcg gattctgata gggggcataa
      181 agtcaagcca ggcacttttg cagccactga cggactgggc aggacagtac ctggatatga
      241 agaagtggga gatgttaggg aaggtaaata tgtaggcatg tttttctgga catggcatgc
      301 atctttttca ggcactgtac caagaaatgt aacggagata ttaaaggaac atcccgacat
      361 catacatgac tacaatgcaa agcagtgggg tggaccaaac gattcatact tctggaacga
      421 gcctatatat ggatactaca atggaaagga tccttgggtg tatagaaaac aggcagagct
      481 tcttgccgac gcaggagtag atgtaataat atttgacaac agcaacaatg tagctacttt
      541 catcgatggt gttaaagtgc tgctggaaac atttgcacag gcaagagctg atggtgtgaa
      601 cactccccag atatcttttc taatgaattt ttttgaagca gactggtcga gcaccgtaat
      661 ccagttgaag gaattgtata aagagatata caaaccagga aagtacaagg agctgtggtt
      721 ttactggaaa ggaaaacctc tgatgatggc ataccctgac aaactgaatc caaatgatgc
      781 gctggaagcg gagattctgg agttctttac atacaggccg gcacagcctt cctacacaat
      841 tgggcagatc cgcccaaacc aatggggatg gtgttctgtt tatcctcagc aggtatacaa
      901 ggatgaaaac ggcaatccgg agcagataac agtcagcgtg gcacaaaacc acaatgacaa
      961 gcgcgggctg actgccatga acggtgaaag ggtattcgga agacactgga taaatgcaaa
     1021 gaaggattat gataccagag aaaatgccat tctttatgga gcgaactttg aagagcagtt
     1081 taattacgct cttgaagtgg atccggaatt catattcatt acaggctgga atgaatggag
     1141 agcgggaaga tacagggaat ggtgcggtgt tcaaaatgca tttcccgatc agttcaatga
     1201 cgcctattca agagatattg agccttccaa aggggatctt aaagaccact actattatca
     1261 gcttgtatcg tttattagaa aatacaaggg tgtggaaaaa cctgagagtg catcagcagg
     1321 caaaactatt gacatatatt ccgaccagga catgtgggag gatgtaaaac catattttgc
     1381 agcatatggc ggtaatacat tccacagaaa cagccctggt tacgttggat atcactatga
     1441 aaacacgact ggaagaaacg acataacagg ggcaaaggtt acctttgaca gggattacgt
     1501 atatttcatg gttgagacca aggaaaatct tactccgcat acggataagg cctggatgag
     1561 actgtttata gatgtagaag ggcagaatgg acctgactgg gaaactttcg agtacgtagt
     1621 aaacagggta aatccaggag aaaaagcagt actggagaaa tctactggtg gatggaattg
     1681 ggagaaagtc ggtgatgtgg attacgctgt taaagacaac agacttcaga taaaagtacc
     1741 aaaatccctg ctgggtattg tgggtgaaaa gtttgtaatt aatttcaaat ggtcggacaa
     1801 tatgcagaac gaaggtgata ttatggactt ttatgtaaac ggtgatgtgg ctccaggcgg
     1861 caggttcaag taccagtatg ctagttacga gctatacaag gaatctactt tttctttagg
     1921 aaaagtaatt accattgttg gtgctgttct ttttgtaggt cttgcggtac tgtcaggtat
     1981 tttggttgta aagaaaagaa agaagtaatt tgggagggat aatatgaaga aagcaattat
     2041 tcttgtgttt ttaatttcat tgtttgtgtt ttcaggatgc gccgacaaga atgcaggtca
     2101 agaatcaacg ccgacgcctg aggcgacaca tactccagat cccaccagta cacccacacc
     2161 aacacaggag ccaaccccaa agccaactcc gatgcctaca gttgaacctg gaacaggtaa
     2221 atatgcagga gaagtggacg aaaccaatgg cgtaaaagcg gatttttacg aaggaggctg
     2281 ggacggcgaa caggcacaca gggtgaatgg aagtctttgc attcagtttt ttgccaccac
     2341 aacctttgac aaagttgggg tttgcatgcc tacttggact caaaagcagg gtcatgcggc
     2401 aacaatttct ctttacgctt ggcaaggttc ctttgatgca acgttaatga aagatcctat
     2461 tgccactaag gaagtggcag attgggcgga tggtgtggaa gttcttctgg aactggatga
     2521 agaaatgccg gatggcgagt atttgtatga gatatataac gacaatacta ataatgtcgg
     2581 tatatggtat aagcctggta ctgtagacta tgtgagaacg tattacaaca atgagatttg
     2641 ggatactggg acacccaggt tggttgtgta ttatacaaaa acgcctgagc atttgcatgg
     2701 gccaattcag gattccggtc ttgagtaaat ttcaagaggt gacgacatgt atggaatgga
     2761 tgagtttttc gaaagtgtaa aagaattgaa tgaggaagaa ctgttctaca aggagtatta
     2821 tatcagataa aagtaccaaa atccctgctg ggtattgtgg gtgaaaagtt tgtaattaat
     2881 ttcaaatggt cggacaatat gcagaacgaa ggtgatatta tggactttta tgtaaacggt
     2941 gatgtggctc caggcggcag gttcaagtac cagtatgcta gttacgagct atacaaggaa
     3001 tctacttttt ctttaggaaa agtaattacc attgttggtg ctgttctttt tgtaggtctt
     3061 gcggtactgt caggtatttt ggttgtaaag aaaagaaaga agtaatttgg gagggataat
     3121 atgaagaaag caattattct tgtgttttta atttcattgt ttgtgttttc aggatgcgcc
     3181 gacaagaatg caggtcaaga atcaacgccg acgcctgagg cgacacatac tccagatccc
     3241 accagtacac ccacaccaac acaggagcca accccaaagc caactccgat gcctacagtt
     3301 gaacctggaa caggtaaata tgcaggagaa gtggacgaaa ccaatggcgt aaaagcggat
     3361 ttttacgaag gaggctggga cggcgaacag gcacacaggg tgaatggaag tctttgcatt
     3421 cagttttttg ccaccacaac ctttgacaaa gttggggttt gcatgcctac ttggactcaa
     3481 aagcagggtc atgcggcaac aatttctctt tacgcttggc aaggttcctt tgatgcaacg
     3541 ttaatgaaag atcctattgc cactaaggaa gtggcagatt gggcggatgg tgtggaagtt
     3601 cttctggaac tggatgaaga aatgccggat ggcgagtatt tgtatgagat atataacgac
     3661 aatactaata atgtcggtat atggtataag cctggtactg tagactatgt gagaacgtat
     3721 tacaacaatg agatttggga tactgggaca cccaggttgg ttgtgtatta tacaaaaacg
     3781 cctgagcatt tgcatgggcc aattcaggat tccggtcttg agtaaatttc aagaggtgac
     3841 gacatgtatg gaatggatga gtttttcgaa agtgtaaaag aattgaatga ggaagaactg
     3901 ttctacaagg agtattatag aataaagaaa gagagtccgg aaaaacttgg tgaatatctc
     3961 gaaatgctgg atagggaaat gatgcaggaa aaggggctgt ggcttcctga aatcaggcag
     4021 acttctgcaa gttactttac cgagtatttt gtagaggaca ttagaaagaa tgtggagata
     4081 agcatacata acagatacac gcctcttttc ttgcacaagc atgccttttt tgagatggtt
     4141 tatgtatatt caggtcaatg tgaaaatact attgatggca acaagattgt catgaacaaa
     4201 ggtgatattt gcataatcgc acctgacgta agccatacaa tgggagtgtt tgatgacgac
     4261 agcattgttt ttaatattat cattcgaagg agcacgttca gggaaatatt tgaaggcatt
     4321 tttatcgatg atacggcttt gtcgctgttt tttaacagga tactgtactc aaatacaaca
     4381 aacagtcaca tactgtttgt ctcggaagat tatgaatcac tgcacagtat tatcaagttc
     4441 ctgatttggg aaggcagcaa aaaagaaaag tactacaagc ctgccatgga gaatctttta
     4501 aggacggtct tttgcttaat gcttagaaga caggaatgca tggttgtttc aagctccctc
     4561 attaccgaag atccggaaat tcttcacatt ctaaggtata ttcaggataa ttataaaact
     4621 gtgaacctga acgacctttc agacaagttc aagtattccc ctgattatat ggggaaactg
     4681 ataaaaaaga atacaggcac cacgttcata aaaatcctga gggatataaa gtttaaaaaa
     4741 gcgtgcaaca tgcttttgaa tacaaatcta aatgtcattg atatttgcta tgaggtgggg
     4801 tatgaatctg ttgaatattt caccaggtcc ttcaaaaagc tttgcggcat gacacccacc
     4861 gaatacagaa agcaggcatc aaatctcaaa aacatacctc catctcaaag aagtattgag
     4921 ttaaatacga ttacctaaac aagtctgctg tttatattat ttattacata aaacccattt
     4981 ctgttgcagg agtcgtagcg gttatacaga agctcggttt ggttcatgtt cagctgacgc
     5041 attatgcctc ctgcttcctc tatgatgatt tgacctgctg cggtatccca ttccatggtg
     5101 tggccgtatc tgtagtatac gtctgccttt ccctctgcaa ttgtgcacat ttttatagcg
     5161 cttccttttg atacgagttt ttcaatctta tgcatgttgt ctgcaagcag ctttttggtt
     5221 tcattctcaa agctgctttt actgtgcatg catatgagtc tttccgttct tccgcttact
     5281 gatattcgtc ttttatcatc aaatatgtta tcaaaaaact ccttgctggt tgatttatac
     5341 gcaccatgac ctcttgctgc atagtacact ttgttaaaca caggaatata tatcataccg
     5401 caaactgctc ttttcttgta tgcaagggca atattaattg aaaactcatc attctttttg
     5461 atgaattctt ttgtgccgtc aagagggtca attattaagc agtatggatt taaaaccctt
     5521 tcctcaatgg tctttttatc atacgtttcc tcggcaagta ttgcatactc agggtatttt
     5581 tccattaaat aatttgatat tatactgttt gatgctctgt ctgcctcggt aaccgggg
//
LOCUS       DAONVY010000087         5601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10660_length_5601_cov_3.428056, whole genome shotgun sequence.
ACCESSION   DAONVY010000087 DAONVY010000000
VERSION     DAONVY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10660_length_5601_cov_3.428056"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLI11_03535"
     CDS             <1..346
                     /locus_tag="PLI11_03535"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPZ51967.1"
                     /translation="YPTPDGTGVRDYIHVVDLAKGHVKAIEYIENYNTIESINLGTGK
                     GYSVLEMVHTYMRVNNVAVNYIFTDRRPGDIAECYANPEKALRLLNWKAEYDLEKMCR
                     DSYLWSVSATKR"
     gene            351..893
                     /locus_tag="PLI11_03540"
     CDS             351..893
                     /locus_tag="PLI11_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPZ51968.1"
                     /translation="MLNKKNTGGIFLFINSILYLLLSVFYIVTPLHDFLSHIIVTGTV
                     LMVCGLVCITAFISNPRAYFRPGWILSQGFLQILLGFYILYNRLVTNYDSYFIIFGLW
                     ALFTGIAQISVSIQIRALDISRWHFVFSIGLFNLIIGMLMMLNLFKFIDLLFVYTAIY
                     LMSLSISYVLEFFVYRKGSA"
     gene            897..1625
                     /locus_tag="PLI11_03545"
     CDS             897..1625
                     /locus_tag="PLI11_03545"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HPZ51969.1"
                     /translation="MNEKNIDIFKLKIIGMITMTILNLALSGIIKNYYITIPMGIIGC
                     MSITIFAFLITEGYRHTSSLNKYMLRVLFVAVISAYPYQWVFGTAKFDPRNFFNSALT
                     AFICLGSLYMYDRMKSKETKIFFLVFITSISIILSFEWAPFAILLTLILHIYKEKEKI
                     MYFYIITGYTVLFMVSLVTLLMNPEYSNEAELVNGIRQIGCIFALPLIKKYNGNLGRC
                     FKWPLYFYYPILLLTLFFIKNLLV"
     gene            complement(1606..2970)
                     /locus_tag="PLI11_03550"
     CDS             complement(1606..2970)
                     /locus_tag="PLI11_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPZ51970.1"
                     /translation="MSDDGSIPLQLLVIFILILLNAFFAASEIALLSVNETKMKKMAE
                     KGNKKARHVVKFLENSSKFLSTIQVGVTFSGFLSSAVAADSFATRIVEFVKAQNASPA
                     IVNVSRTASVIIITFILSYFTLVFGELVPKRIAMNKSEKLALFVIGPLRVTASVFSPF
                     IKILTLSVNGVLRLVGINPHDDDEEVTEEEILLMVNEGQEKGVIGNEESTMISNVLLL
                     NDKTASDIMTHRTEVVGIRADSGYETVMQTVTNARYSRFPVYEDRIDNIVGVIHIKDL
                     LNIKNESEFDLVKLMRAPCFVPETQRIDEVFKTLKNTKNHMAIVVDEYGGTAGIATME
                     DILEELVGNIMDEYDEEEEAEYDRQIVAIGDNSYIIEGLTELDVVNEEFNINLPVEEY
                     DTISGFVIGNLGSIPDENTHPSFEYEGMRFTVEGNNEKRITLVKMELIEDTTIYDDVT
                     QEDF"
     gene            complement(3123..>4409)
                     /locus_tag="PLI11_03555"
     CDS             complement(3123..>4409)
                     /locus_tag="PLI11_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51971.1"
                     /translation="ESQKQDLVYYFNKGKKVYKDGFVFGEPQDKFVLRAGDTRDTGYT
                     MYKERVTTTVSNFVGLEAPGYENKALYSKFPHKYAYLTHIQNDTTVLTKRSIFFYGIT
                     RVSDDAGQINPNYIVRDDGYIATADGEYKIDINGYLLYEGDVWKDIDGNIIELFEYDD
                     DFNLVLADRFDENGRIRFLRPGDQLFLYRVKDLNGDGTYDESEILTDELDRKIQGEPV
                     NGIPARKNKITKITIEIDALGTKLYEGNIEEDPQQQNEIILTLDGWYEQILADQHNAE
                     KDKKGNILEPEKIKGQILTSVKSITTKTSANFTDKDLTTYKSIELDLTEEELNSLPPQ
                     AYIYLTFKINQHQPEEAFNESQTEKLVCIQVTAPVYEDPVKETNWLLIGAIGGGALIV
                     VVAVVIIIVVVASKKKKTTATVDAPAEATTEQPDNE"
     assembly_gap    4410..4419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4420..4627)
                     /locus_tag="PLI11_03560"
     CDS             complement(<4420..4627)
                     /locus_tag="PLI11_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51972.1"
                     /translation="MGKKLFILFLTLVVILSAFFYPVIATEDEENTEGNGEEVEVGPG
                     GLDFYSITIYYDIPTVVDYTYVSRS"
     gene            4981..>5601
                     /locus_tag="PLI11_03565"
     CDS             4981..>5601
                     /locus_tag="PLI11_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPZ51973.1"
                     /translation="MNFDLQHPADQLVMIMERIYAYGMTTTSGGNLSILDDNGDVWIT
                     PGGIDKGSLTRNDIVRIKADGTIEGIHKPSSELPVHLGIYNTRGDIRAVLHAHPPSLV
                     AFSIARKIPETKLIPNVAQICGTVSMAPYDVPGSTGLSKKIAAEFEKGHNVVMLENHG
                     VFVGATDLFKAFMAFETYDFCARLEIEGRCIGNIRALSDKHIQMSKE"
BASE COUNT         1716 a         1068 c          974 g         1833 t
ORIGIN      
        1 ttaccctaca cctgatggca caggagttcg agactatatt catgttgttg atttggccaa
       61 gggacatgtc aaagcaattg aatatataga aaactacaat acaattgaaa gcattaattt
      121 agggacagga aaaggttatt ccgttcttga aatggtacat acctatatga gggtaaacaa
      181 tgttgctgta aattatatat ttacagatag aagacctggg gatattgcgg agtgttatgc
      241 aaatcctgaa aaagcattaa gattgctcaa ctggaaggct gagtatgatt tggaaaaaat
      301 gtgccgagac agttatcttt ggagtgtaag cgcaaccaag aggtgatatg atgttgaaca
      361 aaaaaaatac aggaggaatc tttcttttca taaacagcat attgtacctg cttctttctg
      421 tgttttatat agtaacaccg ttacacgact ttttgtcaca tataattgta accggtactg
      481 tgctcatggt ctgcggtctt gtatgtatta ccgcatttat aagtaatcca agagcatatt
      541 tcagacctgg atggatactg tctcagggat ttttgcaaat cctactagga ttttatatcc
      601 tctacaacag acttgtaaca aactacgatt cgtatttcat catttttggt ctttgggcat
      661 tgtttacagg aattgcccaa atatcggttt caattcaaat acgggctctt gatattagca
      721 gatggcattt tgtattctca ataggtctat tcaatctgat aataggtatg cttatgatgc
      781 tcaacttgtt caaatttatt gatcttttgt ttgtttacac cgctatttac ttgatgtcac
      841 ttagtataag ctatgtattg gagttctttg tatatagaaa gggtagtgca taaacgttga
      901 atgaaaagaa tattgatatc ttcaaattaa agataattgg aatgattaca atgacaatac
      961 ttaatctggc attgtcaggc ataatcaaga attattatat tacaatacca atgggcatta
     1021 tcggttgtat gtccattacc atttttgctt tcttaatcac cgaaggatac aggcatacat
     1081 ctagtttgaa caaatacatg ttaagagtgc tgtttgtggc cgtaatatcc gcatatcctt
     1141 atcaatgggt attcggcaca gcaaaattcg atcccaggaa ctttttcaac tccgctctta
     1201 cggcattcat ttgtcttggt tctttataca tgtacgacag aatgaaaagc aaagaaacaa
     1261 agatattttt tctcgtgttc attaccagta tatcaataat tctcagtttt gagtgggcac
     1321 cctttgcgat cctgcttaca ttgatattgc atatatacaa agaaaaggaa aaaataatgt
     1381 atttctacat catcacagga tacacggtgc tttttatggt ttctcttgtt acactattaa
     1441 tgaatcccga gtattccaat gaagccgaac ttgttaatgg tattcgccaa ataggttgca
     1501 tttttgcatt gcctttaata aaaaaatata acggcaacct tggcagatgc ttcaagtggc
     1561 cgttatactt ctattatcca atacttttgt taactctatt ctttatcaaa aatcttcttg
     1621 tgtaacatca tcatagatgg ttgtatcttc aattaattcc attttaacga gcgtaattct
     1681 cttttcgtta ttaccttcca ctgtaaacct catgccttca tattcgaatg aagggtgagt
     1741 gttttcatcg ggaattgaac cgagattacc aatgacaaag ccactgattg tgtcgtattc
     1801 ctcaacaggg agattgatat tgaactcctc gtttacgaca tcaagttcag tgagtccttc
     1861 gattatgtac gaattatcac caattgcaac tatttgcctg tcatattcgg cttcttcctc
     1921 ttcatcatac tcatccataa tatttccaac cagttcttcc aaaatatctt ccatagtcgc
     1981 aatgccagca gttccgccgt attcatcaac aacaattgcc atatggttct tggtattctt
     2041 caaggtcttg aacacttcgt caatcctttg agtttcaggc acaaagcagg gcgctctcat
     2101 gagtttcaca agatcaaact cactctcatt ctttatattt agcaaatcct tgatatggat
     2161 aacccctact atattatcta tcctgtcctc ataaacaggg aagcgtgaat accttgcgtt
     2221 agttacagtt tgcatgacag tttcatagcc gctatcggcc ctgataccaa caacttcagt
     2281 tctatgggtc atgatgtcag aagccgtctt gtcattcaac agaagcacat tgctaatcat
     2341 tgtgctttct tcatttccaa taacaccttt ctcctgaccc tcatttacca tgagcagtat
     2401 ctcttcctcg gtcacttcct catcgtcatc atgaggatta atgccgacta atcttaatac
     2461 gccattaact gacaatgtaa gaattttaat aaaaggggag aagacagatg cagttaccct
     2521 gagcggtcca ataacaaaca gggctagttt ctcactcttg ttcatggcaa ttctctttgg
     2581 tacaagttcg ccaaagacaa gtgtgaaata tgatagaata aatgtgataa taattacaga
     2641 tgctgttcta gatacattta ctatggccgg ggaagcattc tgagctttta caaactctac
     2701 aattcttgtt gcaaaagagt ctgctgctac agcacttgat aaaaagccag aaaaagtcac
     2761 accaacttgt attgtagata aaaatttact agaattctct agaaacttta ctacatggcg
     2821 tgctttcttg ttcccttttt cagccatttt tttcattttt gtctcgttta cggaaagtaa
     2881 agcaatttca gatgctgcaa aaaatgcatt caataaaatt aaaataaaaa ttactagcaa
     2941 ctgcaaagga atacttccat catcactcat tcggttaatc atttcctttc aataataaaa
     3001 tacaaataaa ttataattaa ataaaattaa attgtcaact taataaaaag agaatgcgct
     3061 atgaaatgcg cactctcaag gtatggttgt tattataata ttattctttc aattgtcttg
     3121 ccttactcat tatcaggttg ttctgtagtt gcctcagctg gtgcgtcaac tgtagccgtg
     3181 gtcttctttt tctttgaagc tactacaaca attataatca ctactgcgac tactacaatt
     3241 aatgctccgc cgcctattgc tccaattaat aaccaattgg tttctttcac tggatcttca
     3301 taaactggag cagttacctg tatacatacc aacttctctg tctgactctc gttgaaagct
     3361 tcctccggtt gatgctgatt aatcttgaag gtcaagtaga tatatgcttg aggcggtaac
     3421 gagttaagtt cctcttccgt taaatccagt tcaatactct tgtaggttgt aagatcctta
     3481 tctgtaaagt tagcggaagt ctttgtagta atggacttta cagaagtcaa gatctgacct
     3541 ttaatctttt ctggttcaag gatgttgccc ttcttatcct tctcggcatt gtgttgatca
     3601 gccaaaatct gctcatacca gccgtcaaga gttagaatga tttcattctg ctgttgtgga
     3661 tcctcttcga tatttccttc ataaagctta gttccaagag cgtcaatttc tattgtaatc
     3721 ttggtaattt tattctttct tgcaggaata ccgtttacag gttcaccctg aatcttacga
     3781 tcaagttcgt cagtaaggat ttcagactcg tcataagtac cgtcgccgtt aaggtcctta
     3841 actctgtata ggaataattg gtcacctggt ctcaggaacc taatcctgcc attttcatca
     3901 aatctgtccg caagaaccag attgaagtca tcatcatatt caaacagctc aataatgttg
     3961 ccgtcaatat ctttccaaac atcgccttca tagagaagat atccgttgat atcgatcttg
     4021 tattcgccgt ctgcagtagc aatatatcca tcatctctaa ctatgtagtt gggattgatt
     4081 tggcctgcat cgtcgctaac ccttgtaata ccatagaaga atattgacct cttggtcaaa
     4141 acagtcgtat cattctgaat atgggttaag taagcgtatt tatgagggaa cttggaatat
     4201 aacgccttat tttcgtagcc gggtgcttca agtccgacaa agttactaac tgtagtagta
     4261 actctctctt tgtacatggt gtaacctgta tcccttgtat caccagcgcg aagaacaaat
     4321 ttgtcttgtg gttcaccgaa tacaaagcca tccttgtata cctttttacc cttgttgaag
     4381 taatatacca aatcttgctt ctgggattcn nnnnnnnnnc acttcttgat acataagtgt
     4441 aatctacaac cgtgggaatg tcatagtaga tggtaataga gtagaaatcc aagccaccag
     4501 gaccaacttc cacttcctca ccattaccct ctgtattttc ctcatcttca gtagcaataa
     4561 caggatagaa aaaggcggat aaaattacga caagtgtaag aaataatata aaaagctttt
     4621 tacccataat gtagcctcct taataatatt tgaaccggag aaaaccggtt aatacctgag
     4681 tattaagatt ttattcacta ttttacttta ttattgtaat atgatattaa cactttgtca
     4741 atatgacaat atatttttat tagcatcacc tataatttat cataaataaa tctgtttgtc
     4801 actagcaaaa ataaaatttc attgatattt tttgatccat gctttatgaa tatgaatcat
     4861 atgcattaat tttaacttaa atgtgaatat atgcaaaata attatcaaga aatctaatat
     4921 tgatactcat taaaaatttt ggtaatatta gagaaagata taaaaaagga gcatgattgt
     4981 atgaactttg acttgcaaca tcctgcagac caattggtaa tgataatgga aagaatttat
     5041 gcctatggta tgacaacaac atcaggaggc aatctgtcaa tcctggatga caatggagat
     5101 gtatggatta cacccggtgg aatagacaaa ggctcactga caaggaacga catagttaga
     5161 ataaaagcag atggtacaat tgaaggaatt cacaaacctt caagcgaact tcctgttcat
     5221 ttgggaatat acaatacaag aggggatata agggctgtcc tgcatgctca tccgccatca
     5281 cttgtggcat tcagcatagc caggaagata ccagaaacaa aactgatacc taatgttgca
     5341 caaatctgtg ggacggtttc aatggctcct tacgatgtac ctggtagtac tggattaagc
     5401 aagaaaatag ctgctgaatt tgaaaaggga cataatgttg taatgcttga aaaccatggc
     5461 gtttttgttg gagctacaga tctgtttaaa gcattcatgg cctttgaaac ttacgatttt
     5521 tgcgcaaggc ttgaaataga gggcagatgc ataggaaaca tacgtgccct ttcggacaag
     5581 catatacaaa tgtcgaaaga a
//
LOCUS       DAONVY010000088         5592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10679_length_5592_cov_3.248691, whole genome shotgun sequence.
ACCESSION   DAONVY010000088 DAONVY010000000
VERSION     DAONVY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10679_length_5592_cov_3.248691"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLI11_03570"
     CDS             <1..315
                     /locus_tag="PLI11_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51974.1"
                     /translation="EILAWSKYKWQHWWEKSMAYPDGIDVGKVDNVIGGMVCAWGDVL
                     AGYDSGDKAAKEEFEFIRARIPALAVKTWDEDSDMDSKDFYESYEKLDALLERILVNA
                     NN"
     gene            302..1123
                     /gene="murQ"
                     /locus_tag="PLI11_03575"
     CDS             302..1123
                     /gene="murQ"
                     /locus_tag="PLI11_03575"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPZ51975.1"
                     /translation="MRITESINKRTNDIDKCETIEMVRMINDEDKSVAFAVEKELQNI
                     ALAIDKAALSLQNGGRMFYVGTGTSGRIGVLDASECPPTYGVSEEMVKAILAGGIEGI
                     TNAANGDEDSHEAGMKDLQKEGVNKKDVVVGITASGRTPYVIGAINHAKSMGALTIGI
                     CNNPDTELSRIADITIAAITGPEVIQGSTRMKAGTAQKMILNMISTGVMVKLGRTCGN
                     IMIDLDANNEKLYGRAENIVCNLTGTNKDTARKALSACEYNIREAVKLIENGDVK"
     gene            1120..2034
                     /locus_tag="PLI11_03580"
     CDS             1120..2034
                     /locus_tag="PLI11_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPZ51976.1"
                     /translation="MKCVIGIEGGGTKSVMRLSDMHGNTIADSHGDCLNIYIIGRESV
                     FNNLKDLYNKIVSKVDFKPDIVRVCIGTAGLVGEDASKFYVSSLQNICNCNDVIALTD
                     AHISLYGYLGDMPGISIISGTGSICIGKNSEGKIVTCGGWGHIFSDEGSAFDIAINAL
                     RYIYGYVDNRNGSALIYSEFLKATSSKDYNDLLTFVYDENTNKTVLASLAEVVNDCAE
                     KGDKIALRILYDAAGKLYDMCYDAAKNLNFNDDYEFGVVASGSVLKKSMIVRNELIKR
                     MQENFKQCKIVKNECDAVSGAVYLALMK"
     gene            complement(2072..4543)
                     /locus_tag="PLI11_03585"
     CDS             complement(2072..4543)
                     /locus_tag="PLI11_03585"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPZ51977.1"
                     /translation="MELFVNKLKEPINIDVNPVFGWTLDAEDFYQKAYQVKVYSGDET
                     VWDSGKTLSDNSVCVEYEGNKLKSNSEYRVEVTVWDTKGNEYKGETRFESALLSQSDW
                     HGNWIGMGDKYISSWAFQLRKEVELEDKKIRRAKAFVVGLGYYELRLNGEKVGDHMLD
                     TAQTEYDETVFYATYDITDKLKKNNCFGIIVGDGWYHQSQLMNGAGIYGKPCAIMQVN
                     IEYEDGTTSEVVTDWTWKVDYSPISRNNVYTGETYDARLEQEGWDEPGFDDSKWFEVV
                     EDTRPKGKLISQLMPPIRVVKEIKPVSITHPQRGVTVFDLGENFAGLVRLKTIGFPGN
                     EIVMRFAESLDETGMIDVTSSGVFHIRGVQTIRYIVGKQGEFTYTPKFTYHGFRYVEL
                     TGDFASQTVETITGLKMNTDLRQTGHFECDMPILNTLHQLLYNAFTSNAYGIPTDCPA
                     REKCGWTGDANVIADTSMIIWDSFHFWDKYMMDTRDSKKVYGIWQNVMPGKRGCLDTV
                     PAWGCEMINIPWYTYLTYGDKGILERNIEQMIAYHKYIHENTTDLIHNDHKYPLADWA
                     APYGYNSKEHFKQTSTAFFYMATVIIAETLKVLEKKEDVVEYEKLAERIKKKFIETFY
                     DFENHTYGTQTLNSFCIELGLYDEGEDVKMCKWIVDDIKKHDGHLTCGHLGLRYIFNV
                     LTKYGYNEVIKEFFEKDDYPSFGEQIKHGATTLYENWGVSNNQSYNHHFKGAFGYWLY
                     NDVLGIKNTGIAYKTFDVKPMTIDIIKKAKGSIDTVYGRIEVDYSVGEYFKVKIPANT
                     TATVYVPNKDLTYVKHVLSSGTYSF"
     gene            complement(4606..5436)
                     /locus_tag="PLI11_03590"
     CDS             complement(4606..5436)
                     /locus_tag="PLI11_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPZ51978.1"
                     /translation="MHNIELNNGVMIPGIGFGTYKTDYTTIKLAIDCGYRYFDTASKY
                     ENEEHVGYAIKDSGIPRQEFFVATKAWRDELGYHEAKKAFNSSLERLQMDYVDLYIIH
                     WPKPTHDYEGWAELVMETWQAFEEIYKEKKARAIGLSNFLPHHLDVILESCEIKPMTN
                     QLEVHPGYSQDYALAYCKENNILVQGWSPLGRERVLNHPLILELASKYKVTSAQICIR
                     YSIQRGVVPIPKASSVERMKNNLDVFTFEIDKSDMDRLITLPQTGWSGEHPDFERVPV
                     "
BASE COUNT         1584 a         1112 c         1159 g         1737 t
ORIGIN      
        1 gagatacttg cctggagcaa gtacaagtgg caacactggt gggaaaagtc aatggcctat
       61 cctgatggaa ttgacgtggg caaagtggac aatgtaattg gtggaatggt ttgcgcctgg
      121 ggagatgttc ttgccggcta cgatagtgga gacaaggcgg cgaaggaaga gtttgaattt
      181 attcgagccc gtattcctgc cctggcagta aagacgtggg atgaggacag cgacatggat
      241 tcaaaagact tttatgagtc atatgagaag cttgatgctc tattggaaag gatattggta
      301 aatgcgaata actgagagca taaataaaag gaccaatgat attgataaat gtgaaaccat
      361 tgaaatggtc cgtatgataa atgacgaaga caagagcgtt gcctttgcgg ttgaaaaaga
      421 actccagaac atagctttgg caattgacaa ggcagcgctc agcctgcaaa atggcggacg
      481 catgttttac gttggtacgg gcaccagtgg cagaatagga gtgttggacg caagtgaatg
      541 cccccctacc tatggtgttt ctgaagaaat ggtgaaggca atactggcag gtggtatcga
      601 gggtattacc aatgcggcca acggcgatga agacagccat gaagcaggga tgaaggactt
      661 gcaaaaagag ggtgtaaaca aaaaagacgt agtagttgga attactgcaa gcggaagaac
      721 tccttatgta attggagcga taaatcatgc aaaaagcatg ggcgcattga ccataggaat
      781 atgcaacaat ccggatactg agctgtcaag gattgctgat attacaattg ctgcaataac
      841 aggtcctgaa gttatccaag gttctacaag aatgaaagca gggactgctc agaagatgat
      901 tcttaacatg atatctacag gggtcatggt aaaactggga aggacttgtg ggaatataat
      961 gattgatctg gatgcaaaca acgaaaagct ttatggtcgg gctgaaaata ttgtttgcaa
     1021 tctgactggt acaaataagg atacagcaag aaaagcgcta tctgcttgtg aatacaacat
     1081 aagagaagca gtaaaactaa ttgaaaatgg ggatgttaaa tgaaatgtgt tattgggatt
     1141 gagggtggag gcacaaagtc cgtcatgaga ctttcagaca tgcatggcaa tacgatagct
     1201 gattctcatg gggattgcct taatatatat attattggca gggaaagcgt cttcaataat
     1261 cttaaggatt tatacaataa aatagtaagc aaagttgatt ttaaacctga cattgttcgt
     1321 gtgtgcatag gaacagctgg gttggtggga gaggatgcaa gtaaatttta tgtttcttct
     1381 cttcagaata tatgcaactg caacgatgta atagccctca ctgatgcaca tatatctctc
     1441 tatgggtatt tgggggatat gccgggtata tctataattt caggaactgg ctctatttgc
     1501 attggaaaaa acagtgaagg caaaattgta acatgcggtg gctggggaca tatattctcc
     1561 gatgaaggct cagcgttcga tattgctatt aatgcattaa gatatattta cggttatgtt
     1621 gacaatagga atggcagcgc actaatatat tcggagtttt taaaggctac cagctcaaag
     1681 gattataatg atttgcttac gtttgtgtat gatgaaaata ccaacaagac tgtattggcc
     1741 tcattggctg aagtggtaaa cgactgtgct gaaaaaggcg acaaaattgc attacgcata
     1801 ctttatgatg ctgcaggcaa gctgtatgat atgtgttatg atgccgctaa aaatcttaat
     1861 tttaatgacg attatgaatt tggcgtggtc gcaagcgggt cggttttaaa aaaatccatg
     1921 atagtaagga atgaattaat aaagaggatg caggagaatt ttaaacagtg caagatagtg
     1981 aagaatgaat gtgatgctgt ttcgggtgcc gtctatcttg cattaatgaa atgaaaaaac
     2041 ccacaaggag tttgccttgt ggggcttttt cttagaagct gtaagttccg cttgacagta
     2101 catgttttac atatgtcaga tctttgtttg gtacataaac tgtagctgtg gtattggccg
     2161 gtatttttac cttgaagtat tctcctacag agtagtcaac ttcaatcctt ccgtaaaccg
     2221 tatcaataga accctttgct tttttaataa tatcaatcgt cattggcttt acatcaaagg
     2281 tcttgtatgc aatacctgta ttcttaatac caagcacatc attatacagc cagtaaccga
     2341 aagcaccctt gaagtggtga ttgtaggact ggttgttgct gactccccag ttttcgtaca
     2401 aggttgtggc accgtgctta atctgctccc caaaggaagg ataatcgtcc ttttcaaaga
     2461 actcttttat aacctcattg tatccgtatt tggttaatac attgaagatg tatctaagcc
     2521 ccaaatgtcc acaggtcaga tgtccgtcat gtttctttat gtcatctaca atccacttgc
     2581 acattttcac atcttcacct tcgtcataca aacccagctc tatgcaaaaa ctgtttagtg
     2641 tttgtgtacc gtatgtatgg ttttcaaaat catagaaggt ttcaataaac ttcttcttaa
     2701 ttctttcagc cagtttttca tactcgacaa catcttcttt tttctcgagt accttcaagg
     2761 tttcagcaat aattacagtt gccatataga agaaggctgt tgatgtttgc ttgaaatgct
     2821 cttttgagtt gtaaccataa ggagccgccc agtcggcaag aggatactta tggtcgttgt
     2881 gtataaggtc tgtggtgttt tcgtgtatgt acttgtggta tgcaatcatc tgttcaatgt
     2941 ttctttccaa tatgccctta tcaccataag taaggtatgt ataccatggg atattaatca
     3001 tttcacaacc ccaggcagga accgtatcaa ggcatcctct ctttccgggc attacattct
     3061 gccatatgcc gtataccttc ttggaatccc ttgtatccat catgtatttg tcccagaagt
     3121 ggaaagaatc ccatataatc attgatgtat cagcaataac attggcatct cctgtccagc
     3181 cgcatttctc tcttgcaggg cagtcggtgg gaattccgta cgcatttgag gtaaaagcat
     3241 tgtaaagaag ttggtgcagt gtgtttagaa taggcatgtc acactcgaag tggcctgtct
     3301 gcctcaaatc cgtgttcatc ttgagtccgg ttattgtttc aacagtctgg ctggcaaaat
     3361 caccggtaag ttctacatac ctaaagccat ggtaggtaaa ctttggtgta taagtaaatt
     3421 ctccctgttt gccaactatg tatcttattg tctgcacacc ccttatatga aacacaccgg
     3481 agctggttac atcaatcatt ccggtttcat caagactttc tgcaaacctc ataactattt
     3541 cgttgcctgg aaaacctatt gttttaagcc tgacaagacc tgcaaagttc tcacccaagt
     3601 caaagactgt aacacctctt tgaggatgag ttatagatac aggcttaatt tccttgacca
     3661 ccctaattgg cggcatcaac tgtgatatta gtttgccttt cggtctggtg tcttcaacaa
     3721 cttcaaacca ttttgaatca tcaaagccag gctcgtccca gccttcctgc tcaagccttg
     3781 catcatacgt ttcgcccgta taaacgttgt ttcttgagat tggggagtag tcaactttcc
     3841 aagtccaatc agtaacaact tcacttgtgg taccatcttc gtattctatg ttcacttgca
     3901 ttattgcgca tggttttcca taaatgccgg caccattcat aagctgggac tggtggtacc
     3961 agccatcccc tacaattata ccaaagcagt tatttttctt aagtttatcg gttatgtcat
     4021 aggtagcgta gaataccgtt tcgtcgtatt cggtctgggc agtatcaagc atatggtctc
     4081 ctactttttc gccgtttagt ctaagttcat aatatccaag accaacaaca aaagctttag
     4141 cccttcttat cttcttgtct tccaattcaa cttcttttct tagttggaat gcccagcttg
     4201 aaatatattt gtctcccatg ccaatccagt ttccgtgcca gtcggactgg gatagaagag
     4261 cactttcaaa ccttgtctcc cctttatact cattaccttt tgtgtcccat actgtcactt
     4321 ccacacggta ttcagaattg cttttcaatt tattgccttc gtactcaaca caaacggaat
     4381 tgtcgctcaa agttttacca ctgtcccaaa ccgtttcatc accggaataa acttttacct
     4441 ggtacgcttt ctgataaaag tcctctgcat ccaatgtcca gccaaagaca ggattcacat
     4501 caatattgat aggttccttc aacttgttta caaacagttc catcaaattt cctccttaat
     4561 aatcaagctt aacttattat actattattt ttaatatata caacctcaaa cgggcactct
     4621 ttcaaaatca ggatgctctc ccgaccatcc tgtttgcgga agagttatta gcctgtccat
     4681 gtcgctttta tctatctcaa aggtgaatac atcaagattg tttttcattc tttcaacaga
     4741 tgatgccttg ggtattggca ctactcctct ttgtattgaa taccttatgc aaatttgtgc
     4801 actggttacc ttgtatttgc ttgccagttc aagaatcaga gggtgattta aaaccctttc
     4861 tcttcccagt ggactccagc cctgtaccag tatattgttt tccttacagt aggcaagcgc
     4921 ataatcctgt gaataaccag gatgtacttc cagctggtta gtcattggtt taatttcgca
     4981 gctttcaaga ataacatcca gatgatgagg aaggaaattg ctaagaccta tagcccttgc
     5041 cttcttttcc ttgtatattt cttcaaaagc ctgccaggtc tccatgacga gttccgccca
     5101 tccttcgtaa tcatgagttg gttttggcca gtggataatg tacaaatcca cataatccat
     5161 ttgaagtctt tcaagggaac tgttaaaagc cttctttgct tcatggtatc ccaattcatc
     5221 gcgccaagcc tttgtagcaa caaaaaactc ttgcctggga ataccactgt cttttatggc
     5281 atatccaacg tgttcctcat tttcatattt ggaggcagtg tcaaagtatc tgtaaccgca
     5341 atcaatggcc agctttatcg tagtatagtc tgttttgtat gtcccaaacc ctatgccggg
     5401 tatcattact ccgttattga gctcaatgtt atgcatatta gcacccatcc tttccttttg
     5461 ggtgtattat acactaaata ttgcttgagg caatgtttta tgttggatta agtcaataaa
     5521 atatgtctta aatgtacata gcgagtttgt tcatgtagga tataatttaa tatgtaagca
     5581 agttgtttcc at
//
LOCUS       DAONVY010000089         5579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10707_length_5579_cov_3.269913, whole genome shotgun sequence.
ACCESSION   DAONVY010000089 DAONVY010000000
VERSION     DAONVY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10707_length_5579_cov_3.269913"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLI11_03595"
     CDS             <1..330
                     /locus_tag="PLI11_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51979.1"
                     /translation="DLTYKIDIIKSLKNLRRIAVSPFADVKKCAEQIGNHYVLSWRPN
                     PSTAVSFGVDEDYVRKELREAFDIFDANKCVFDITLKDVETVSGDENAIVKWTRIVRE
                     EIERRYK"
     gene            complement(342..1799)
                     /gene="pepD"
                     /locus_tag="PLI11_03600"
     CDS             complement(342..1799)
                     /gene="pepD"
                     /locus_tag="PLI11_03600"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HPZ51980.1"
                     /translation="MITLDRVLELFNELNTYPRESGNRKPVADYLCNFAQEKGLEYYR
                     DEYENVVIKKEASKGMENSPSLILQAHYDMVCVKDEDVDHDFSKDPITSYIEDGWLKA
                     RGTTLGADNGIGVAIMLAFLESDTITPYLECVFTSDEETNMEGAKYLDKSILKSRYMI
                     NLDEETEDSITVSSAGISDIRLNYSKNDILEEYVNETKTVVEINIDGLKGGHSGIDIK
                     YNRVNAIKLVCDLLYWLSPFNVEVLDIKAGTHMNAIPKDACVILSVENDTYDNFRKFI
                     DKYTREKQLKYKEAEPGFTLVMQESDRIFDKKLKDSFVTSLIKMVSELPCGALAFERE
                     IKDVVETSANIGRIVSEYDILSVWVSVRSSVDEKLDSLVETITVTGKKYESEVKVGFH
                     EYGWPVNKDSKLVPYLLKIKEERKEKLPKVVGIHAGLECGTFSREIENADICAIGPNI
                     MNPHTTSEKVEVKSIENFVGFLETVINRFDEIERH"
     gene            complement(1817..3082)
                     /locus_tag="PLI11_03605"
     CDS             complement(1817..3082)
                     /locus_tag="PLI11_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPZ51981.1"
                     /translation="MHINTKCVQEGYNPTKGEPRVIPIIQSTTFKYDTAEQLGDLFDL
                     KTDGFFYSRLANPTVAAVERKLASLEGGVGCVLTSSGQSANFFAVLNICQAGDHVISQ
                     SAIYGGTYNLFNNTMKDMGIEFTFLKPDATEEEIESAFRENNKCVFAECLSNPSLNVL
                     DVEMYANYAHKHGVPLIVDNTFPTPVNLRPIEYGADIVTHSTTKYLDGHAVSLGGAII
                     DSGNFNWDNGKYPKLTTPDESYHGIIYTEKFGKGAYIAKLTTHLMRDIGAMMSPQNAF
                     YLNLGLETLFLRMERHCQNAQAVAEFLENHEKVTWVNYPSLKSSSQYEKCMKYMPEGT
                     CGVISFGVKGGRESATKFMNNLKLAAIVTHVADLRTCVIHPASTTHRQMSDEELEVAG
                     VAPDLIRMSIGIEHVDDIIKDIEQSLEKI"
     gene            complement(3192..4670)
                     /gene="spoIVA"
                     /locus_tag="PLI11_03610"
     CDS             complement(3192..4670)
                     /gene="spoIVA"
                     /locus_tag="PLI11_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HPZ51982.1"
                     /translation="MDRYDIYQQIAIRTKGDIYIGVVGPVRTGKSTFIKRFMDLLVIP
                     NISDPYERERAVDELPQSAAGKTIMTTEPKFIPNEAAQITIGGNIQLKVRLVDCVGYL
                     VNNALGYLENDMPRMVDTPWFDDPIPFGEAAEIGTQKVINEHSTIGIVVTTDGSITEI
                     KREDYVEAEEKVIKEMQDTGKPFAILLNCVDPNAEHVPALRDSLYEKYNVPVIPVNCS
                     EMEQSDINLIMRTILYEFPICEIGFNVPQWMESLDYDENLKVELIEAIKEAFADCDRL
                     RSVEECANLIENAQFIKRAQITEVDAGKGTALIELAMPDELFYKILSERSGLDINDDK
                     ALINMIKDLGRIKRAYERLEYALREVELKGYGIVMPTVEEMRLEDPEIVRQGNRFGVR
                     LRASAPSIHMIKADIETEIAPFVGTEKQSEELVNYILSEFENDPNSIWSSNLFGKPLN
                     ELMSEGLSNKLSRIPDDERLKLQETIERVINEGSGGLICIIL"
     gene            complement(4794..5396)
                     /gene="plsY"
                     /locus_tag="PLI11_03615"
     CDS             complement(4794..5396)
                     /gene="plsY"
                     /locus_tag="PLI11_03615"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPZ51983.1"
                     /translation="MIYGIVSIILGYLLGSINSAVLISKVFYKTDIRTMGSGNAGTTN
                     VLRSLGKKAAAFTFLGDFLKGIIASLVGLLLYKEMGLVLAGGAAVVGHNWPAYFGFKG
                     GKGVLTSFSVLLFASPWAALIALAWFIIIVAITRYVSLGSITAALVVVIATYFTGHGF
                     DTPTFWLTVFVAVLVIIRHHSNIKRLLTGTESKLGQKNKN"
     gene            complement(5397..>5579)
                     /locus_tag="PLI11_03620"
     CDS             complement(5397..>5579)
                     /locus_tag="PLI11_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPZ51984.1"
                     /translation="KIYYMTQVKVKPPTFVIFCNDEKLFHYSYLRYIENQMRTNFGFE
                     GTPLVFIVRERNEDDK"
BASE COUNT         1534 a         1230 c          965 g         1850 t
ORIGIN      
        1 gatcttacat ataaaatcga tattattaaa agtcttaaga atttacgaag aatagccgtt
       61 tcaccttttg cagatgtgaa aaaatgcgct gaacagattg gtaatcacta tgttctttcg
      121 tggcggccca atccatccac ggcggtctcc tttggtgtcg atgaagatta tgttcgtaag
      181 gagttgcgtg aggctttcga cattttcgac gcaaacaaat gcgtgttcga tatcacatta
      241 aaagatgttg aaactgtttc aggcgatgaa aatgccattg ttaaatggac tagaattgta
      301 agggaagaaa ttgaaagacg atataaatga aattgaaagg cttaatgcct ttcaatttca
      361 tcaaacctat taataaccgt ctctaaaaat ccaacgaagt tttcaattga cttaacctct
      421 actttttcac tggtagtatg aggattcata atattcggtc ctattgcaca gatgtctgca
      481 ttctctattt ctcttgaaaa tgttccgcat tcaagaccag catggatacc aactactttt
      541 ggcagtttct ccttcctttc ttcctttatc ttcaaaagat acggtacaag ttttgaatcc
      601 ttgtttacag gccatccata ctcatgaaaa cctaccttta cctcggattc atatttctta
      661 ccagtaactg taattgtttc gaccaaacta tcaagttttt catccacaga acttcttaca
      721 ctaacccata cggataaaat atcatactca gaaacaattc taccaatgtt ggcagatgtt
      781 tctaccacgt cttttatctc cctttcaaat gctagtgcgc cacaaggaag ttcactcacc
      841 atttttatta acgaagtcac aaaactatcc ttaagttttt tatcaaatat tctgtctgac
      901 tcttgcataa caagggtaaa accaggttct gcttccttgt attttagctg tttttctcgt
      961 gtatatttgt ctataaattt tctaaaatta tcataagtgt cattttctac agacaaaata
     1021 acacaagcat ccttcggaat ggcattcata tgggttcctg ccttgatatc caaaacttca
     1081 acattgaacg gcgaaagcca atacaaaagg tcgcatacaa gtttgattgc attaaccctg
     1141 ttgtatttaa tatctatacc ggagtgacca cctttcaatc catcaatatt tatctctaca
     1201 accgttttag tctcgtttac gtattcttcc agaatatcat ttttgctgta attcaacctg
     1261 atatcagaaa ttcctgctga agaaacagta atgctgtcct cggtttcctc atcaagatta
     1321 atcatatacc tgcttttaag aatactcttg tcaaggtact ttgcgccttc catgtttgtt
     1381 tcttcgtccg atgtaaagac acattccaga tatggggtta ttgtatcact ttcaagaaat
     1441 gcaagcatta ttgctacgcc gatgccatta tcggctccaa gtgttgtccc tcttgccttc
     1501 agccagccat cttcgatata tgaggttatg ggatctttgg aaaagtcatg gtccacatct
     1561 tcatccttga cacaaaccat gtcataatgg gcttgcagaa tcaatgacgg actgttctcc
     1621 atgccttttg aggcttcttt cttaataaca acattttcgt attcatccct gtaatactca
     1681 agcccttttt cctgtgcaaa gttacacaga taatctgcaa ctggttttct gtttcccgac
     1741 tccctcggat acgtatttaa ctcattgaaa agctcaagta ctctatctag tgtaatcata
     1801 tatcattctc ctatctttat attttctcca aactctgttc aatatccttg attatgtcat
     1861 caacatgctc aattccgatg gacattctga taaggtctgg ggcaactcca gcaacttcaa
     1921 gctcttcatc cgacatttgc ctgtgcgttg tgcttgcagg atggattaca caggtcctaa
     1981 gatctgccac atgagttaca attgcagcaa gcttcagatt gttcatgaat tttgttgcac
     2041 tttccctgcc gccctttaca ccgaatgaaa taacgccgca ggtaccctca ggcatgtatt
     2101 tcatgcactt ttcgtactgg ctgcttgatt tcaatgacgg ataattgacc caagtgactt
     2161 tttcatgatt ttcaagaaac tcggctacgg cctgtgcgtt ttgacaatgt ctttccattc
     2221 ttaaaaacaa agtttcaagt cccagattca gataaaaggc attctgagga gacatcattg
     2281 cgcctatatc tctcattaaa tgagtagtaa gttttgcaat atatgcacct ttgccaaatt
     2341 tctcagtata tataatgccg tgataggact cgtccggagt tgtaagtttg ggatacttgc
     2401 cgttgtccca attaaagttg ccgctgtcta ttattgctcc accaagactt actgcatgtc
     2461 cgtccaaata ttttgttgtt gaatgtgtta ctatgtcagc accatattca ataggtctca
     2521 agtttacagg ggttggaaaa gtgttgtcaa ctataagggg gacaccgtgt ttgtgggcgt
     2581 aatttgcata catttccaca tcaagaacgt tcaaagatgg atttgaaaga cattctgcaa
     2641 atacacattt gttattctct ctgaatgcgc tttcaatttc ttcttctgta gcatcaggct
     2701 taagaaatgt gaattctata cccatatcct tcattgtgtt gttgaataga ttgtatgttc
     2761 cgccgtagat ggcgctttgt gatattacat ggtctcctgc ctggcagatg tttaatactg
     2821 caaagaaatt ggcactctga ccggaagagg ttaaaacgca gccaacccca ccttccaatg
     2881 aagccagttt tctttcaaca gctgcaactg tggggttagc caatctggaa tagaaaaacc
     2941 catctgtttt taaatcaaac aagtcaccca attgttccgc cgtatcgtat ttgaatgttg
     3001 tgctttgaat aatgggaatg actctaggtt ccccctttgt gggattgtaa ccttcttgta
     3061 cgcatttggt atttatgtgc atcaattttc actccatctg ttattataat ccatgatata
     3121 ttatagcata ataatttgaa aaataccgaa aaacttatac aagcttcccg atattttgaa
     3181 atagtattca gttacaagat tatgcagata agtccgccgc tgccttcatt tatgaccctt
     3241 tcaatggtct cctggagctt tagacgttca tcatctggta tcctgctcag tttgtttgac
     3301 agtccttcgc tcataagttc attcaatggt tttccaaaaa gatttgaaga ccagatgctg
     3361 ttaggatcgt tctcaaattc acttagtatg taattcacca actcttccga ctgtttttcc
     3421 gttccgacaa aaggagcaat ttccgtttcg atatcagcct taatcatgtg aattgatggt
     3481 gccgatgctc ttaatcttac accaaatctg ttgccctgtc tgacaatctc cggatcttcc
     3541 agtctcatct cttcaaccgt tggcattaca attccatagc ctttaagttc aacttccctt
     3601 aaagcatatt ccagtctctc gtatgctctc ttgattctgc caaggtcttt tatcatgtta
     3661 ataagagcct tgtcgtcatt tatatcaagt ccggatcttt cggaaagaat cttgtagaac
     3721 agttcatcag gcattgccag ctctatcaaa gcagtacctt tgcctgcatc cacttccgtt
     3781 atctgtgctc tcttgataaa ttgtgcgttt tcaataaggt ttgcgcattc ctcaacagat
     3841 ctcagtctgt cacagtcagc aaaggcttcc ttgatagcct caatcagttc aacttttagg
     3901 ttttcatcat agtccaggct ttccatccat tgaggtacat tgaatccaat ttcgcagatt
     3961 gggaactcat atagtattgt cctcattatc aaattgatgt cactctgctc catttcgctg
     4021 cagttcacag gtattacggg aacattgtat ttttcataca agctgtctct aagtgctggc
     4081 acatgctccg catttggatc aacacaattg agaagaatcg caaaaggctt gcctgtgtct
     4141 tgcatctcct ttattacctt ctcttccgcc tccacatagt cttctctctt tatttccgtt
     4201 atgcttccat cagtggtgac cacaatgcca atggtggagt gctcatttat taccttctgg
     4261 gtgccaattt cagcagcttc accaaatgga ataggatcat caaaccaagg tgtgtcaacc
     4321 attctgggca tgtcgttttc aagataccca agtgcattgt tgacaaggta tccaacacaa
     4381 tccaccagtc ttaccttcag ctgaatgttt ccgccaatag tgatctgtgc agcttcattt
     4441 ggtatgaact ttggctcggt tgtcataatc gttttgccag cagcactctg cggcagctca
     4501 tccactgcac gttctctctc atatggatca cttatgtttg gtatgaccaa aaggtccatg
     4561 aatcttttga taaatgtaga cttgccagtt cttacaggac ctacaacgcc gatatatata
     4621 tcacctttgg tccttatggc aatctgctga taaatatcat atctatccac tatttgaaac
     4681 ctcccttatc atgtttgtct taatacggat acaacatata tatattcctg ttaattgaaa
     4741 atattacaag ctgtcgtttt taatttttca tttatgacaa gggaggcaaa tattcagttt
     4801 ttattctttt gacccagttt tgactctgtt ccagttagaa gtctttttat gtttgaatga
     4861 tgacgaatta ttacaagaac tgctacaaat accgtaagcc agaaggttgg agtatcaaac
     4921 ccgtgccctg taaaatatgt agctattaca actactaaag cagcagtgat tgatccaagg
     4981 gacacatatc tggttatggc aactattatg ataaaccatg ccagcgcaat caatgcagcc
     5041 catggggatg caaacaacag tacggaaaag gatgtaagga cgccttttcc tcccttgaat
     5101 ccaaaatatg ctggccaatt gtgcccaaca actgctgcac cgcctgcaag tacaagcccc
     5161 atttccttgt acaatagcag tccaacaagg gaggctatta ttccctttag gaagtcgcca
     5221 agaaaggtaa aagcagcagc ttttttacca agacttctta acacattggt ggtgcctgcg
     5281 ttgccgctgc ccatggtcct tatatcggtt ttataaaata ctttggatat aaggacggcg
     5341 gagtttatgc tgccaagcaa atagcctaag atgatggaaa ctataccgta aatcatttac
     5401 ttatcatcct catttctttc cctgactatg aagaccaaag gagtaccttc gaatccaaaa
     5461 ttggttctca tttgattttc tatatatctc aaatatgaat agtgaaaaag tttttcgtca
     5521 ttacagaata tgacgaatgt aggcggtttt accttcactt gcgtcatata ataaatctt
//
LOCUS       DAONVY010000090         5574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10719_length_5574_cov_3.651386, whole genome shotgun sequence.
ACCESSION   DAONVY010000090 DAONVY010000000
VERSION     DAONVY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10719_length_5574_cov_3.651386"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..2449
                     /locus_tag="PLI11_03625"
     CDS             131..2449
                     /locus_tag="PLI11_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPZ51985.1"
                     /translation="MRNWDLYIIPNHEVTNKKFNPKTMSELKENYKSIPAKVPGNFEL
                     DMMEAGLIEDPFYGTNVLKMQELENLHLFYATVFDNLENREENSKTYLVFEGIDTIAD
                     IFLNGIFLGHVDNMFIAHEFLVEEYLRDKNNELLVHIYPATIEARKYELTPLNFALRY
                     NYASLRIRKAASMFGWDIMPRIVSGGIWKEVKLVQKKNERIEDIYIYTLSASPESSTA
                     YIRAFYNIETDADLIKDLVIKVKGVCKDSTFEMSQRLFHTYGAMSITVNNAMLWYPKN
                     AGEPHLYDTTVSLYRGDLLIDKKKLKIGIRTVDLVRTSTTDESGNGEFVFKINGRKVF
                     CMGTNWVPLDAFHSNDMNRLPKALELMDDIGCNMVRCWGGNVYEDHAFFDFCDEKGIM
                     VWQDFAMGCGVYSQDEEFQKKIEKEAAYIVKKLRNHPSLVLWAGDNECDLFQVIHNNI
                     VDPNDNIITRKTLKEVCRIHDFARPYLPSSPYIDDFAFESKKPLSEDHLWGPRDYFKG
                     SFYKNSLCHFASETGYHGCPSPNSLKKFIPEDKLWPMFEENGNTVPEWQIHASCMETE
                     MGVEFSYRIGLMADQVKTLFGSLPDNITTFAYMSQISQAEAKKYFIERFRISKWRRTG
                     ILWWNLLDGWPQISDAVVDYYFVKKLAYHYIKRSQSPLCLMCDEPKNNLLPLYAVNDT
                     QYDTLLKYTVTRFSDNLVIAEGVFNAPHDSSCIVCNIPIKENEKDFYYIEWKDNKGRK
                     HFNHYFTNIIDIDFDMYMSALKRYNMNQFTHF"
     gene            2514..2792
                     /locus_tag="PLI11_03630"
     CDS             2514..2792
                     /locus_tag="PLI11_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51986.1"
                     /translation="MSTPENTPSITPQSQAIANLIESLATQHASLAKLIDNEGSKIQA
                     VLDMGCCTVDEMLSVNQSVSDTLNKVIKLEMVLDLKLEEIRKLSESIN"
     gene            2912..3436
                     /gene="dcd"
                     /locus_tag="PLI11_03635"
     CDS             2912..3436
                     /gene="dcd"
                     /locus_tag="PLI11_03635"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HPZ51987.1"
                     /translation="MILSGKEIEKRIGKDIVIEPFNKKQLNPNSYNLRLHNELLVYED
                     DILDMKKENKARKIIIPEEGLVLEPNKLYLGRTAEYTKTSNLVPMLEGRSSIGRLGLF
                     IHVTAGFGDVGFSGYWTLEMFCIQPVKIYPYIECCQIYYHTIEGEFEEYKSGKYQNNT
                     GIQPSLLYKDFIKN"
     gene            complement(3433..4890)
                     /locus_tag="PLI11_03640"
     CDS             complement(3433..4890)
                     /locus_tag="PLI11_03640"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPZ51988.1"
                     /translation="MKKLDWSDTRNLTMLMDFYELTMANGYMESGLEGISACFDMSIR
                     RIPDGGGFAIMAGLEQVIENLKELRFKEKDIEFLRSKNLFSDKFLEYLRNFKFSCDVW
                     AVPEGTPVFPNEPIVKVVGPIVEAQLLETLVLVLINHQSLIATKSNRIVRAAQGRAVM
                     EFGSRRAHGADSAIYGARAAYIAGCVGTSCVLSSKMFGIPALGTMAHSWVQLFDTELD
                     AFLAYARAFPSSCTLLVDTYDVLRSGIPNAIKAFEQEVVPRGFRPYAVRIDSGDIVQL
                     SKKAREMLDEAGFEDCKIVASNSLDEYSIQEMLSKGACVDQFGVGERLITSASDSVFG
                     GVYKLVAIKRDGEIIPKIKISETTGKINIPGVKELHRLYDNHTNEAVADIISLRGEEI
                     DVTKPYEAYDVYEKDKKVVLKNFSTKKLLTPIFEKGKCVYDSPNIEEIKSYCRQQVDT
                     LSNTIKSLKNPEHYPVYLSPGVWQLKNELFGEKKV"
     gene            complement(5010..>5574)
                     /gene="fucI"
                     /locus_tag="PLI11_03645"
     CDS             complement(5010..>5574)
                     /gene="fucI"
                     /locus_tag="PLI11_03645"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459161.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HPZ51989.1"
                     /translation="KDEKGNGVIKPFWEMSDEDIQACLKATTWNAADRGYFRGGGFSS
                     RFVTRAEMPATMFRLNIVKGLGPVLQLAEGYTINLPDEVTDILWKRTDYTWPCTWFAP
                     RLTGSGVFKSAYDVMNNWGANHAVVSYGHIGADLITLCSMLRIPVCMQNVDEDKMFRP
                     ASWNAFGMDKEGQDYRACLTYGPLYKK"
BASE COUNT         1733 a         1197 c         1052 g         1592 t
ORIGIN      
        1 ctcacccctt aatgtatata ttttacattt tttctgcttt cttccttatg ccgatgtaga
       61 gcaatttcct ataacacaaa gaaaggcaga attctgcctt ctttttttta tgtttagcga
      121 ggtatagaaa atgagaaact gggacttgta cataatacct aatcatgaag ttacgaataa
      181 aaagtttaat cctaaaacca tgagcgaact caaggaaaac tataaatcga tacctgcaaa
      241 agttcctggc aactttgaac tggacatgat ggaagcaggt cttatcgaag atccttttta
      301 tgggacaaat gtgttgaaaa tgcaggaact tgaaaacctg catttgttct acgccacagt
      361 attcgacaac ctggaaaaca gggaagaaaa ctcaaaaaca taccttgtat ttgaaggcat
      421 tgataccatt gcagatattt ttttaaacgg catcttttta ggccatgtgg acaatatgtt
      481 tatcgcccat gagtttttgg ttgaagaata tctaagagat aaaaacaatg agcttttggt
      541 tcacatatat ccagccacaa tcgaagcaag aaaatatgag cttacaccac taaactttgc
      601 ccttagatac aattatgcta gcttaagaat aagaaaagct gcatccatgt ttggctggga
      661 cataatgcct cgtatcgtat ccggcggcat ttggaaagag gtaaagcttg tacagaaaaa
      721 gaatgagaga atagaagaca tttatatcta tacattatcc gcttctcccg aaagcagcac
      781 tgcatacatt cgggcattct acaatattga aaccgatgca gacctgataa aggatttggt
      841 gattaaagtc aaaggagtat gcaaagattc cacttttgaa atgtctcaaa ggctttttca
      901 cacctatggt gccatgtcca taacagtaaa caatgccatg ctatggtatc ccaagaatgc
      961 aggagagcca catctttatg atacaacggt ttccttgtac agaggagatt tgcttattga
     1021 caagaaaaaa ctaaagattg gcataaggac agttgatctt gtcagaacct ctacaaccga
     1081 cgaatcagga aatggagagt tcgtgtttaa aataaatggt agaaaggttt tctgcatggg
     1141 gacaaactgg gtgcctctcg acgcatttca ttcaaatgat atgaatagac tgccaaaagc
     1201 tttggagctt atggatgaca taggatgcaa catggtcaga tgctggggcg gcaatgtata
     1261 tgaggatcat gctttctttg acttctgcga tgaaaaagga ataatggtgt ggcaggattt
     1321 tgccatgggt tgtggtgttt attcacagga tgaagagttt cagaagaaaa tagaaaagga
     1381 agctgcatac attgtaaaga aattgagaaa tcatccttct cttgtattgt gggcaggaga
     1441 caatgagtgt gatttgtttc aagtcattca taacaatata gtggacccaa atgacaacat
     1501 aattacacgc aaaaccctaa aagaagtctg cagaatccac gattttgcac gaccctatct
     1561 cccaagctcc ccatatatcg atgattttgc atttgagtcc aaaaagcctt tgagtgaaga
     1621 ccatctatgg ggacctagag actatttcaa aggcagcttt tacaaaaaca gcttatgcca
     1681 ctttgcgtct gaaaccggtt atcacggctg tccttcaccc aactctctca agaagttcat
     1741 tcccgaagac aagttatggc ccatgtttga ggaaaacggc aacaccgtac cggaatggca
     1801 gatccacgct tcctgtatgg aaacggaaat gggtgttgag ttttcataca ggattggact
     1861 tatggcagat caggttaaga cccttttcgg atcccttcct gacaatataa ctacctttgc
     1921 atatatgagt cagatttctc aggcagaggc aaaaaaatat tttatagagc gcttccgcat
     1981 ctctaaatgg agaagaacag gtatcttgtg gtggaatctg cttgacggct ggccacagat
     2041 ttccgatgca gtagtcgact actatttcgt caaaaagctg gcctatcatt acataaaacg
     2101 atcccaatcg cctctttgcc ttatgtgcga cgaaccaaaa aacaacctgc ttcccctgta
     2161 tgcggtaaat gacactcaat atgatacact tcttaaatac accgtaacaa ggttttcaga
     2221 taacttggtt atagcggaag gtgttttcaa tgcgccgcat gatagctcat gcatagtatg
     2281 caacattccc atcaaagaaa acgaaaaaga cttctattat atcgaatgga aggataacaa
     2341 gggaagaaag cactttaacc attatttcac caatataata gacatagact tcgacatgta
     2401 catgagtgcg ttgaagcgat acaatatgaa ccagtttacg catttttagg ggtaaccact
     2461 ttaatgtttt ccaaatatca tatactagaa cgaattttgg aagtgaagaa aaaatgtcaa
     2521 cccctgaaaa cactccatca attacgcctc aatcgcaagc catagccaat ctgattgagt
     2581 ctcttgcaac gcaacatgcc agtctggcaa aacttattga caacgaagga agcaaaattc
     2641 aggcagttct tgatatgggt tgctgcaccg ttgacgaaat gctttcagtc aaccaatccg
     2701 tatcggatac tctaaacaaa gtcatcaaac tggaaatggt gcttgacctc aagcttgaag
     2761 aaatcaggaa attgagcgaa agcatcaatt aagaaaagtt aaatatttgc atgggatacg
     2821 gagattgttt taatcttcgt atttttctct tgcgctttat atgttttacg attacaatac
     2881 taattcacta aaaatatgag gtgcaagaga catgatactg tcaggaaaag aaatagagaa
     2941 aagaatcggg aaagatatag ttatagagcc gtttaataaa aagcagttga atccaaacag
     3001 ttacaactta agactgcata acgagctgtt ggtttatgaa gatgatattc tggatatgaa
     3061 aaaggaaaac aaggcaagga aaatcataat ccctgaagaa ggccttgttt tggaaccgaa
     3121 caaactgtat ttgggaagaa ctgcagagta tacaaagaca agcaacctgg taccaatgct
     3181 tgaaggcagg tcctcaatag gcagacttgg ccttttcatc catgtcactg caggttttgg
     3241 tgatgtggga ttctccggat attggacctt ggaaatgttc tgcatacaac ctgtcaaaat
     3301 ttatccatat atagagtgct gtcaaatata ctaccatacc atagaaggtg agtttgaaga
     3361 atacaaaagc ggaaagtacc agaacaatac gggcatacag ccaagcctgt tgtacaagga
     3421 ctttatcaaa aactaaacct tcttctcccc aaacaactcg ttttttagtt gccacacccc
     3481 aggagacaga tacactggat agtgctcagg gttcttgaga gacttgattg tattgctcag
     3541 agtgtcaacc tgctgtctgc agtagctttt tatctcctca atatttgggc tgtcatatac
     3601 gcatttgcct ttttcaaaaa tcggcgtcaa gagcttctta gtggaaaagt tctttaagac
     3661 aacctttttg tctttctcat aaacatcata cgcctcatat ggctttgtga cgtctatttc
     3721 ctctcccctt aatgaaataa tatcagccac agcctcgttg gtgtgattgt cataaagcct
     3781 gtgcagttct ttgactccag ggatgttaat cttacccgta gtctcactta tctttatctt
     3841 cgggattatc tctccatccc tcttgattgc cacaagcttg tacactccgc caaatactga
     3901 gtcactggca gaagtaataa gtctttcacc aactccaaat tggtcaacgc aagctccttt
     3961 agaaagcatc tcctgtatgc tgtactcgtc aagagaattt gaagccacaa tcttgcaatc
     4021 ctcaaaacca gcttcatcaa gcatttctct tgccttcttt gacagttgca caatgtctcc
     4081 actgtctatc cttacagcat aaggtctgaa acctcttggc actacttctt gctcaaatgc
     4141 cttaatggca tttggtatac cgcttcttaa tacatcataa gtgtcaacca aaagtgtgca
     4201 tgagctcggg aaagctcttg catatgctaa aaatgcatcc agttcagtat caaagagctg
     4261 aacccatgag tgcgccattg tcccaagggc tggaatccca aacattttac ttgaaagaac
     4321 acaggatgtt cccacacacc ctgctatata cgcagctctt gctccataaa tggcgctatc
     4381 tgcgccatgg gctcttcttg agccaaactc cataacagcc cttccctgag cagcccttac
     4441 aatcctgttt gatttggtag caataaggct ttggtggtta atgagtacaa gaaccaaagt
     4501 ctccaacagc tgcgcttcca caataggacc aacaaccttt actattggtt catttggaaa
     4561 cactggagtt ccttcgggta ctgcccatac atcacaggag aatttgaaat ttctaaggta
     4621 ttccaaaaat ttatctgaaa aaaggttttt gcttcttaaa aactcaatat ctttttcctt
     4681 aaatcgaagc tctttcaaat tttcaatcac ttgctcaaga cctgccataa tggcaaaacc
     4741 gccaccgtca ggtatccttc taatggacat gtcaaagcat gctgaaatac cttcaagtcc
     4801 actttccata tatccgtttg ccatagtcaa ctcgtaaaaa tccatcaaca tggtaagatt
     4861 cctggtatct gaccaatcca actttttcat ctcttgcggc tccttatgct tgtttattgt
     4921 ccacacatta gtttatacca aatccacaaa attgtaaata atataagggg acgcctgata
     4981 tgcgtccctt aatacatact attgtttatt tactttttat acagggggcc atatgtcaga
     5041 catgccctgt aatcctgtcc ttccttgtcc attccaaaag cattccaact ggcaggcctg
     5101 aacattttat cctcatccac attttgcata cacactggta ttctcagcat cgagcacaag
     5161 gttattaaat ccgcacctat atgtccataa cttacaactg catggtttgc accccagttg
     5221 ttcattacat cataagcgct cttgaatacc ccactgccgg taagtcttgg agcaaaccag
     5281 gtacaaggcc atgtataatc tgttctcttc cacaagatat cagtaacctc atccgggaga
     5341 ttgatggtgt atccttcagc aagttgtaaa acagggccca atccctttac aatattcagc
     5401 cgaaacatgg tagcaggcat ttctgctcta gttacaaatc ttgaagagaa tcctccacct
     5461 ctgaaatacc ccctgtcggc tgcattccaa gttgttgcct taaggcatgc ctgaatatct
     5521 tcatcagaca tctcccagaa tggtttgatg actccattgc ccttctcatc cttt
//
LOCUS       DAONVY010000091         5567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10734_length_5567_cov_3.505261, whole genome shotgun sequence.
ACCESSION   DAONVY010000091 DAONVY010000000
VERSION     DAONVY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10734_length_5567_cov_3.505261"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="PLI11_03650"
     CDS             complement(<1..2076)
                     /locus_tag="PLI11_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPZ51990.1"
                     /translation="MYEKCDFSVIENPRILNVNKCPSRAHYIPHNCRCEAYGCESELS
                     DSYMLLNGKWRFKYFERLVDAVHSLNDGIDDFDTIRVPSNWQMHGYDKPHYTNAAYPI
                     PLDPPYVPNENPAAIYEREFKLPEGFKGTDIYLNFEGVDTYFFVWINKQFVGASQGAH
                     LNSEFRITEKVKEGKNTITVLVVKWAWSTYLEDQDFFRLSGIFRDVYLLSRPLVHIHD
                     VEVRTRNDGTIDIKYNVFKEGKFSDENVEVTFELFDDKAQVIAFGNGSNGEGQLKIDS
                     PVLWNAENPYLYTLLISCNGEIIPIKLGFREVSISQRGELLINGTPIKIKGVNRHDTH
                     PVLGHYTPYNEMKKELVIMKQHNINAIRTSHYPNTPAFYSLCNELGFYVIDEADLETH
                     GTAFGGNLDGVDMSLMLTNNPDWDAAFVDRMERMVERDKNFPCIIMWSLGNEAFFGEN
                     HIKMSQWTKARDNTRPVHYERGDTHSCLDIYSRMYSSVDFVLDRGEEGLSAERENAPL
                     DDVRRKPFFLCEYSHAMGNGPGDLKDYWELFYKYPRLIGGCVWEWADHAVYKKENGQD
                     VYLYGGQSGEYPHDGNFCVDGLVFPDRTPSPGLLELKNVIGPVTVEAVDLGKGVIKVT
                     NRYDFTDLSVLEGRYKIVAQTSTFQSGTFTVSCKPHETVEVNLDYKLPSISFEEYFLD
                     ISFVIKNDTS"
     gene            2194..4146
                     /locus_tag="PLI11_03655"
     CDS             2194..4146
                     /locus_tag="PLI11_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51991.1"
                     /translation="MLGVLVYIIYLFLGILLADIMFDKKRPLLKFWAGLVVGTVLLMW
                     SNVPFSFIFGFTIVSHVLGLLSSIAIYALLFFFKSKRSEDFHIKSYISFFKSRWFVPL
                     EKSEKSFLIIISGLMVYSLICLLNHTIYEFEGAIWTGQCTYGDMNMHLGFITSIATQG
                     KFPPEYSILPGMKLNYPFLCDSVSSSLYLFGSSLRTAYILPMLVAFFSVFAGFWFTAA
                     SILKKTRKTIVAFMLFFLNGGFGLIYFLDNLGNNNKNLTRIFTNFYETPTNLVNKGTS
                     YTNIRWTNTIVDMMLPQRATLFGWMVLFFVLYLLYNAVFEDKKEYFIPAGIFAGLIPM
                     IHTHSYFACGLVAIGWIIVTVIKERFSKEIIKSWLMFGVPALLISLPQLLIWTFDAAL
                     GNESFVRFMFNWVNETDNWLWFWVKNVGVCFILLPLAFLNTSRKNKLMYSGALVIFII
                     GEFFVFQPNPYDNNKLFLIWYAFTAILVAEFMVNAYNKMKGIKGRQVIAGFMIFLCIN
                     AAVLTMAREIISGFRPYSYQLYGKDHVDAAEYIIENTEKDATFLSHNNHNNAIASLTG
                     RNIFCGAGTFLYFHGVGYQERESMLNGFFTNVNTFEQYKDVYGIDYVYVSSYEKSNYK
                     GIILDHLEQNYEKVFAQGEVAIYKVR"
     gene            complement(4148..5248)
                     /locus_tag="PLI11_03660"
     CDS             complement(4148..5248)
                     /locus_tag="PLI11_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPZ51992.1"
                     /translation="MIEIVGKYNVAKVFTDNMEHEAYNQIKELCDMKFAKDSKIRIMP
                     DVHTGIGCTIGTTMTIKDKVVPNLVGVDIGCGMETVRIREKKLNLQKLDNLIYARIPS
                     GRNVRRNSHKLADDIDLKKLKCFKHINHGRALMSIGTLGGGNHFIEASTDDEGNLYIV
                     VHSGSRHLGNEVARYYQNEGVRTLNDSGVPKHLAYLEGKLFDNYIHDMNIIQQYAVLN
                     RKAMMEEILNGMNLTAEERFTTTHNYIDTEKMILRKGSVSAEKGEKLLIPINMRDGSL
                     ICIGKGNADWNYSAPHGAGRIMSRRQAKQVFSLNEFKREMEGIFTTSINKDTLDECPM
                     AYKDMDEIIKHVGDTVEIVKNIRPIYNFKAAE"
     gene            5418..>5567
                     /locus_tag="PLI11_03665"
     CDS             5418..>5567
                     /locus_tag="PLI11_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HPZ51993.1"
                     /translation="MLILDILQEISTYLQQGRAKNVKTLVQEALDAGIAADKILQEGL
                     LHGMDI"
BASE COUNT         1586 a         1159 c          991 g         1831 t
ORIGIN      
        1 ggatgtatca ttttttataa caaaggaaat atcaaggaaa tattcttcaa aagatatgga
       61 aggcaacttg taatcaagat ttacctctac tgtttcatgc ggtttgcaag aaacagtaaa
      121 tgtaccgctt tgaaaagttg aagtttgtgc aacaattttg taccttcctt ccaatacgga
      181 caaatccgta aagtcatacc tgtttgtaac ctttataact ccctttccaa ggtcaaccgc
      241 ctctaccgta acaggaccta taacattttt cagttccaga agtccggggc ttggtgttct
      301 gtctgggaaa accagtccgt caacgcagaa gtttccatca tgtggatact ctccgctctg
      361 tcctccatac aaatatacat cctgtccatt ctccttttta tatactgcat gatccgccca
      421 ctcccataca cagccaccaa taagtcttgg gtatttataa aagagttccc agtaatcctt
      481 gaggtctccc ggtccattgc ccatggcatg gctgtactcg cataagaaga agggcttcct
      541 tctaacatca tccaatggag cgttttctct ttcagcagac aagccttctt cgcctctatc
      601 caaaacaaaa tcaacacttg aatacattct actgtatata tcaaggcaag agtgcgtatc
      661 tcctctttca taatgtacag gtcttgtatt gtcccttgct tttgtccact gactcatctt
      721 gatgtggttt tcaccaaaaa aggcttcatt gcccaaagac cacattatga tgcaggggaa
      781 attcttgtcc ctttcaacca ttctttccat tctgtctaca aaggctgcat cccagtccgg
      841 gttgtttgtc agcatcaatg acatgtcaac gccatctaga ttgccaccaa aagcagtacc
      901 atgggtctcc aaatccgcct cgtctataac gtagaagcca agttcgttac acagactata
      961 aaaggcgggg gtgtttggat aatggcttgt tcttatggca tttatattgt gctgcttcat
     1021 tataacaagt tctttcttca tttcattgta tggtgtatag tgtcccagaa caggatgagt
     1081 gtcatgcctg ttaacgcctt ttatcttgat gggagtacca tttatcaaaa gctctcctct
     1141 ttgagaaatc gaaacttctc taaaaccaag ctttattggt atgatttctc cattacatga
     1201 aatcagcaag gtatataaat atgggttttc agcattccag agaacagggg agtctatttt
     1261 tagctgtcct tcaccattac tgccattacc aaacgctata acctgagcct tgtcatcaaa
     1321 aagctcaaat gttacttcaa cattctcatc actaaacttg ccctctttaa atacattgta
     1381 ttttatgtct attgtaccgt cattcctggt ccttacttcc acatcatgaa tgtggacaag
     1441 tggtctcgat aaaagataca catctctaaa aatacctgac agcctgaaga aatcctgatc
     1501 ttctagataa gtggaccagg cccatttgac tacaagcacg gttattgtgt ttttcccttc
     1561 tttaaccttt tccgttattc taaactcaga attaagatga gcaccttggg aagctccaac
     1621 aaactgtttg tttatccaaa caaaaaagta ggtatctaca ccctcaaaat tcagataaat
     1681 atctgtcccc ttaaatcctt caggaagctt gaactccctt tcataaatag cagcaggatt
     1741 ctcattggga acataaggtg gatccagagg aatagggtag gcagcgtttg tataatgggg
     1801 cttgtcatat ccatgcatct gccaatttga aggcactctt atagtatcaa agtcgtcgat
     1861 accatcattc aatgaatgta cagcgtcgac aagtctttca aaatatttaa atctccattt
     1921 tccgttgaga agcatgtagc tgtcgctgag ttcgctttca cagccatatg cttcacacct
     1981 gcaattgtgt gggatatagt gtgcccttga tgggcatttg ttgacattca aaattctcgg
     2041 attttctata actgagaaat cacacttttc gtacaccgtt ccatcctcct aaaattttgt
     2101 tatcttgatt atatcaattg aaatgaatct tgaaaagtga taatatagat acaaattgtt
     2161 tataaataaa ttattgaata ttgggaggac tttgtgctgg gagtattagt ttatattatt
     2221 tatcttttct tgggtatttt gttggcagat ataatgtttg acaagaaaag gcctctgctt
     2281 aagttttggg cgggattggt agttggaacc gtccttctta tgtggtccaa tgtgcctttt
     2341 tctttcatat ttggttttac cattgtctcg catgttcttg gtcttttatc aagcattgca
     2401 atttatgcac tgctcttttt ctttaaatcc aaaagaagcg aagattttca cataaaatca
     2461 tatatttcat tcttcaagtc aagatggttt gttccattgg aaaagagtga aaaatccttt
     2521 cttattataa tatccggatt aatggtttat tcactcatat gtcttctaaa tcataccatc
     2581 tacgagtttg aaggcgccat atggacaggc cagtgcacct acggcgacat gaacatgcat
     2641 cttggattca taaccagcat tgcaacacag ggtaaatttc ctcctgaata cagcattctt
     2701 ccaggaatga aactgaacta tccgtttcta tgcgactctg tttcatcatc tttgtacctc
     2761 tttggttcaa gtctgagaac agcatacatt ttgcccatgc tggttgcttt tttcagcgtg
     2821 tttgctggtt tctggtttac agccgccagc atactgaaaa agacaagaaa aaccatagta
     2881 gcttttatgc ttttcttctt gaatggaggt ttcggactta tatacttcct tgataattta
     2941 ggaaacaaca acaaaaacct aacaaggata tttacaaact tttatgaaac tcccaccaat
     3001 cttgtaaaca aaggcacttc atatactaat attaggtgga caaacacaat agtcgacatg
     3061 atgcttccac aaagggcaac cctctttggc tggatggtac tcttttttgt attgtatctt
     3121 ctttacaacg cagtgtttga agacaaaaaa gaatacttca tccctgcagg catattcgca
     3181 ggccttattc caatgataca cacccattca tactttgcct gcggtcttgt tgccattgga
     3241 tggataattg taactgttat caaggaaagg ttttcaaagg aaatcatcaa atcatggctc
     3301 atgtttggcg tacctgcgct tttaatatca ctgcctcaac tgctgatttg gacctttgat
     3361 gccgcactgg gtaacgagtc gtttgtcaga tttatgttca actgggtaaa cgaaacagac
     3421 aactggctct ggttctgggt gaagaatgta ggagtttgct ttatactgct tcctttggcc
     3481 tttcttaaca catcaagaaa gaataaactg atgtattccg gtgcattagt gatatttata
     3541 ataggggaat tcttcgtctt ccagccaaat ccctatgata acaacaagct tttccttata
     3601 tggtatgcct ttactgcaat acttgtagct gagttcatgg ttaatgcata caacaagatg
     3661 aagggcatca agggaagaca ggtaattgca gggtttatga ttttcttgtg cataaacgcc
     3721 gcagtactta caatggcaag agagataata tccgggttca gaccgtattc atatcagctt
     3781 tatggaaaag accatgtaga cgcagcagag tatataattg aaaacacaga aaaagatgcg
     3841 acatttctgt cgcacaacaa tcacaataat gccatagctt cgttaaccgg tagaaacatt
     3901 ttctgtggtg caggtacatt tttgtacttc cacggtgttg gatatcagga aagagaaagt
     3961 atgcttaacg gcttttttac aaacgtaaac acctttgaac agtacaagga cgtgtacggt
     4021 attgattacg tttatgtaag ttcctatgaa aaaagcaact ataaaggaat tatcctggat
     4081 catcttgagc agaactatga gaaagttttt gctcaagggg aggtagcaat atacaaggta
     4141 aggtaggcta ttcggcagcc ttgaagttgt aaattggtct tatgtttttc acaatctcta
     4201 ccgtatcacc tacatgtttg attatttcat ccatatcttt ataagccatg ggacactcgt
     4261 cgagggtgtc cttgtttatt gaggttgtga atattccttc catttccctt ttaaattcat
     4321 tcaatgaaaa tacctgtttt gcctgtcgtc tgctcatgat tctacctgcg ccatggggtg
     4381 ctgaatagtt ccagtctgca ttgccctttc ctatgcatat taaacttcca tccctcatgt
     4441 tgatgggaat caatagtttt tctccttttt ctgccgatac acttcctttt cttaaaatca
     4501 ttttctccgt atcgatataa ttgtgggttg tggtaaacct ttcttccgca gtaaggttca
     4561 taccgtttaa tatctcttcc atcatggctt tcctattcag aacagcatac tgctgaatta
     4621 tattcatatc atgaatgtag ttatcaaaca actttccttc caaatatgcc aaatgcttgg
     4681 gaacaccaga atcatttaaa gttcttactc cttcattttg gtagtagcgg gcaacttcat
     4741 ttcccaaatg tctgcttcct gaatgcacaa caatgtacaa gtttccttca tcatcagtgc
     4801 tggcttcaat aaagtggttg ccaccgccca atgtaccaat tgacatcaaa gcccttccgt
     4861 gatttatatg tttgaaacac tttagtttct ttaaatcaat atcatcagct aacttgtggg
     4921 aatttcttct tacattcctt cctgatggga ttctagcata aatcaagtta tccagctttt
     4981 gaagattcag cttcttttcc cttattctca ctgtttccat gccgcaaccg atatccacac
     5041 ccaccaaatt gggcacaacc ttgtccttaa ttgtcatggt ggtgcctatt gtacacccaa
     5101 tacccgtatg tacgtccggc attatgcgaa tctttgaatc ttttgcaaac ttcatgtcgc
     5161 acaactcttt tatttgattg taagcttcat gctccatatt gtctgtaaac acttttgcca
     5221 cgttgtattt tccaacaatc tctatcaact ttttcacctc caagatataa gtatagaaaa
     5281 tatgggaatt atgtcaactt aacatatgca tcaaaaatat ttatataata caaaaacatg
     5341 tcgcatttgt ataatttatt tattgacaga gctattgtgg tgtgtaataa tgaatgagaa
     5401 tttaaaaaac gattggggtg ttaatattgg acattttaca agaaatatcc acatatttgc
     5461 agcagggcag agctaaaaac gtaaagacat tggtacagga agctcttgac gctggcattg
     5521 cagcagacaa gatattgcaa gaaggtctgc ttcatggtat ggatata
//
LOCUS       DAONVY010000092         5553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10765_length_5553_cov_4.062568, whole genome shotgun sequence.
ACCESSION   DAONVY010000092 DAONVY010000000
VERSION     DAONVY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10765_length_5553_cov_4.062568"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLI11_03670"
     CDS             <1..350
                     /locus_tag="PLI11_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ51994.1"
                     /translation="AVIHDAEGNEALKVENSRLNPESSQLAIGIRDKAVNIDNLAFTY
                     EFEPTPTPDPTPTPSPTEEVKETEKPATTTAAKSDKTSGDSNMGIIIGIIGAAVVVCV
                     VIILLAVRATKKK"
     gene            complement(388..1056)
                     /locus_tag="PLI11_03675"
     CDS             complement(388..1056)
                     /locus_tag="PLI11_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HPZ51995.1"
                     /translation="MEYFIAFLEGIITFISPCLLPMLPVYVSYFSGQDNTSKKDTFLN
                     SLGFVFGFTTIFVTLGALAGTLGRFLTKYNTYVNILLGLAMILMGLSIAGLFNLPFFK
                     GGKGFNANGKKMNMGRSFVFGVVFSVSWTPCVGAFLGSALMMAASKGESLTGIIMLLL
                     YSIGLGVPFVVSAILIDRIKNTFNFIKRNYKVINIVSGSLLVIFGILLATGLMGHFLR
                     LLSF"
     regulatory      1441..1638
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1657..2607
                     /locus_tag="PLI11_03680"
     CDS             1657..2607
                     /locus_tag="PLI11_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ51996.1"
                     /translation="MIKSIKKSIIIVMAVVLLFVLASCAIAENNPKPKSTPVQTTDDT
                     EYPLTITDTQGNKFKVEKEPEKIITIGPNLTETVYALGKGDKLIGRTDYDDYPNQVSE
                     VESIGDLMTPNVEKVIDLDADIVLVSGTVMPDYINQMRQNSVPVFVIDDQSSFEGVYD
                     TILMIGNILNANSKASEIVEDMKGKVADVTNKVNDLEKPTVYYVVGYGEYGDYTATGD
                     TFISFLLEMAGGDNIAKNEQGWSYSKEALIEKDPELIICSDRWDTKQGFMSTPGYEDL
                     TAVKEGKVFELDENIITRQGPRLADGLLELAKILHPEAFN"
     gene            2629..3861
                     /locus_tag="PLI11_03685"
     CDS             2629..3861
                     /locus_tag="PLI11_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPZ51997.1"
                     /translation="MENNFNEKLREYARLTVRLGVNLQKGQELVLSCPVECAYFGRMI
                     AEEAYIAGAKEVITIWNDELMTKLKYDFADSEVFKEFPNWAVEQRLMYARRGACFVSI
                     AASDPDLLRDVDPARLRDASIASNTALKEFYDYTMSNQCAWTVISVPTERWAKKVFDK
                     GDDSVSLLWDAILKSVRVGGGNAEKAWDEHVETLKRRYEFLNDKQFSYLHFKNSLGTD
                     FKVGLPKNHIWAGGSDVTQSGIPFIANMPTEEIFTAPDRNFAEGRVVSSMPLSYQGTL
                     IENFVLDFKNGEVVNYSAEKGYDSLKNMLESFDGAKRLGEVALVQYDSPISRMGIIFF
                     NTLFDENASCHLALGKAYPASIENGTNMSEEELERHGLNNSLTHVDFMIGTPDLEITG
                     YDEKDNPTPIFRNGNFVI"
     gene            complement(3858..5330)
                     /locus_tag="PLI11_03690"
     CDS             complement(3858..5330)
                     /locus_tag="PLI11_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ51998.1"
                     /translation="MSLWLDTCKLPEYDALDKDIECDVLIMGGGLSGILTAFLLKQKG
                     VDACIVESDRIASGTTGRTTGKVTIQHGLCYHNFLNQIGREKTLMYKIANDEALKTYR
                     YIVDKLNIECDFETNPAYVYTTEPKYAEAIKEEAEVAKDLGIDAEYIEGKLPLPFPTE
                     CAVKFNNQAQFHPLKFISSIIQDLKIYEKTKVLEIDVEKHVARTTGGKIKARHIVFAT
                     GFPFNYTPGYYFLRMHQKRSYLLALEIQSDSPQLDGMYLDANGENGFTLRMYNGMLLF
                     GGGSHRTGENSGGKYDYLIQSANKYYPGCRIVHQWSAQDCVSLDNIPYIGQFSASTPN
                     IFVTTGYRKWGMTGSMVGAKIVADMILGQNDTENYREVFNPQRFNLSASAVNLFKDTV
                     KAVKGLAKEKFTIPKEELKEVNSGEGKVIEYLGKKVGVYRDDEGTVYTVSTKCSHLGC
                     QLEWNQDEKSWDCPCHGSRFDYKGFVIDNPAIHPVNFLDD"
BASE COUNT         1642 a         1054 c         1202 g         1655 t
ORIGIN      
        1 ctgcggtaat tcatgatgct gaaggcaacg aagctttgaa agttgagaat tcaagactca
       61 atcctgaatc cagtcagctg gcaattggta tccgtgacaa ggctgtaaat atagataatc
      121 tggcttttac ctatgaattt gaaccaacac caactccaga tcctacacct acaccttcgc
      181 caactgagga agtaaaggag acggaaaaac ccgcaacgac aactgctgca aaatctgata
      241 aaacatcagg cgatagtaat atgggaataa tcattggaat tatcggagct gctgttgttg
      301 tttgtgtggt tatcatcttg ctggctgtaa gggcaactaa gaaaaagtaa tgacattaaa
      361 gctgccgcca ctgcggcagc tttatgccta aaacgaaagc aatctcagaa aatgtcccat
      421 aagccctgtg gcaagaagta ttccaaatat tacaagtagc gaacccgaca caatatttat
      481 aactttataa tttctcttaa taaaattgaa ggtgttcttt attctgtcta tcagtattgc
      541 gctgactaca aagggaactc caagaccgat ggagtatagc agaagcatta ttatccctgt
      601 aaggctttct cctttggaag cggccatcat cagagctgaa cctaaaaaag ctcccacaca
      661 aggtgtccag cttacagaga atacaacacc aaacacaaag gatcttccca tgttcatttt
      721 tttcccattt gcattaaaac ctttcccgcc tttaaaaaag ggaaggttga aaagacctgc
      781 tatgcttaaa cccataagga tcattgccag ccccaacaat atgttcacat aggtattgta
      841 ttttgtaagg aatcttccca atgtacctgc caaggctccc aaggttacga atatggttgt
      901 aaagccgaaa acaaatccca atgaattaag gaatgtgtct tttttgctcg tattgtcctg
      961 tcctgagaag taggagacgt acacgggcag cattggaaga aggcagggcg atatgaatgt
     1021 gattattcct tcaagaaatg cgataaaata ctccataaac aagaactcct tgtgatttta
     1081 tttatacaag gtacattata ctctgcgaaa aatcaagtac agtgatttag ttacataaaa
     1141 acaaattttg ttgaaaatac atctattttc tgatggtatt acaacaatag aaggtatcgg
     1201 tatttcacaa gtattaattg tcaacatagc tttgaatatg tatatgattg ctaatgttta
     1261 cttgttacca ttgcatcgat accccaataa taccattccg ctggaattga aatatagtgg
     1321 ataaagccct aaaggcttcc tacagaggag aattcggtat aggttgtggt tgatatggag
     1381 atatcgtcga tggaaccctt ttcaatcttt ttttgttgac atatgtattt ttgcgatgta
     1441 atattatgtc tgttcccagg tagctttttg ctttaatagg gaatgcggtg agaatccgca
     1501 gcagcccccg ctactgtatt aacttacgca tgccatggct cgttttgaca accgggtcat
     1561 accactggga aactgggaag gtatggtatg cggcttgagg tttaagccag gagacctgcc
     1621 gggaatgaga aaaataaata atttcggagg gaaattatga taaaaagtat taagaagtcc
     1681 ataataattg ttatggcagt tgttttattg tttgttttgg caagttgtgc cattgcggag
     1741 aacaatccga aaccaaagtc aactccggta cagacaactg acgataccga gtaccccttg
     1801 acaataacag acacgcaggg caacaagttt aaggttgaaa aggaacctga gaagataatc
     1861 acaattgggc ccaatcttac ggaaactgtt tatgctctgg gaaaagggga caaacttatt
     1921 ggcagaacgg attatgacga ttatcctaat caagtttccg aagtcgaatc cataggagat
     1981 ttgatgactc ccaatgtgga gaaggttatt gaccttgatg cggacattgt acttgtttct
     2041 ggaacggtta tgcccgacta tatcaaccag atgaggcaaa atagcgtccc ggtttttgta
     2101 atcgatgatc agagcagttt tgaaggtgtt tatgacacga tcttaatgat tggaaacata
     2161 cttaacgcaa actccaaagc ttccgagatc gttgaagaca tgaagggaaa agttgcagac
     2221 gttacaaaca aggtaaatga cttggaaaag cccacggtat attatgtagt tggatatggt
     2281 gaatacgggg attacactgc tacgggagac accttcatta gttttctgct tgaaatggcc
     2341 ggaggagaca acatcgcaaa gaatgaacag ggatggtcat atagcaagga agctttgatt
     2401 gaaaaagatc ctgagctcat tatctgctct gacagatggg ataccaaaca aggattcatg
     2461 tctacgccag gttatgaaga tttgaccgca gttaaagagg gcaaggtatt tgaactggac
     2521 gaaaacatta ttacaagaca gggcccgcgt cttgccgacg gtcttttgga acttgccaaa
     2581 atactacatc cagaagcttt taattaacta atatgatagg tgatagtatt ggaaaacaat
     2641 tttaacgaga aacttcgtga atatgcccgc ctgacagtaa ggctgggtgt aaacctgcaa
     2701 aaaggacagg agcttgtttt aagctgtcct gtggagtgcg catattttgg cagaatgatt
     2761 gccgaggaag cgtacattgc tggcgccaaa gaagtcatta caatttggaa tgacgagctg
     2821 atgacaaaat tgaagtatga ctttgccgac agtgaagtat tcaaagaatt tccgaattgg
     2881 gctgttgagc aaaggcttat gtatgcaaga aggggagcgt gttttgtaag cattgccgca
     2941 tctgatcctg acttgttaag ggatgtggat cctgcaaggc ttagagatgc atcaattgca
     3001 tcaaacactg cccttaagga attctatgac tacaccatgt caaaccagtg tgcatggact
     3061 gtcatttcag ttcctactga aagatgggca aagaaggtgt ttgacaaggg ggatgactcg
     3121 gtgtcactgt tatgggatgc gattttgaag tctgtcagag ttggaggcgg aaatgcggaa
     3181 aaggcatggg atgagcatgt tgaaactttg aagagaaggt atgaattctt gaatgataag
     3241 cagttttcct acctgcattt taagaatagt cttggcaccg acttcaaggt gggtcttcca
     3301 aagaaccaca tttgggcagg tggtagtgat gtaacacaaa gcggcatacc attcattgca
     3361 aacatgccaa cagaagaaat atttacagct cctgatagga actttgcaga gggcagggta
     3421 gtaagttcga tgccgctcag ttatcaggga actttgatag agaactttgt gctggatttc
     3481 aagaatggag aggttgtgaa ttattcagca gagaagggct atgattcttt gaagaatatg
     3541 ctggagtctt ttgatggagc aaaaagactt ggagaggtgg ctcttgtgca atatgattca
     3601 ccaatatcca gaatgggaat catcttcttc aacacattgt ttgatgaaaa cgcttcctgt
     3661 catctggctt taggtaaagc ttatccggcc agcattgaaa acggcacaaa tatgagtgag
     3721 gaagaactcg aaagacatgg tcttaacaac tccctcaccc atgtggattt catgatagga
     3781 acacctgacc ttgaaattac aggctacgat gagaaggaca atccaacgcc aatatttaga
     3841 aacggaaact ttgttatcta atcgtccagg aagtttactg gatgaatggc agggttgtct
     3901 atgacaaatc ctttgtaatc gaatcttgag ccatggcatg ggcaatccca ggatttttca
     3961 tcctgattcc attccaactg gcatcctagg tgggaacatt tcgttgatac ggtgtataca
     4021 gtgccttcgt cgtctctgta tacacctact tttttgccca gatattcaat aacttttcct
     4081 tctccactgt taacttcttt tagttcctct ttaggtattg tgaatttttc cttggcaagg
     4141 ccttttacag ccttgacagt atccttaaag aggtttacag ctgaagcgga gaggttgaat
     4201 ctttgtggat tgaatacttc ccgatagttt tcagtatcgt tttgaccaag tatcatgtct
     4261 gcaactatct ttgctccaac catggagcct gtcattcccc atttcctgta accagtagtc
     4321 acaaatatat tgggagtaga tgcggaaaac tgacctatat acggtatgtt gtcaagggac
     4381 acacagtcct gggcagacca ttgatgaact attctgcatc cgggataata cttgtttgct
     4441 gattgtatca ggtaatcgta tttgccgccg gagttttctc ctgttctgtg gcttcctcct
     4501 ccaaatagca gcattccgtt gtacattctc aaagtaaaac cgttttctcc atttgcatca
     4561 agatacattc cgtccagctg ggggctgtcg ctttgtattt ccaatgcaag cagataggac
     4621 cgcttttgat gcatgcgtaa aaaatagtag ccgggggtgt aattgaaagg aaaacctgta
     4681 gcaaatacta tgtgtcttgc ttttattttc cctcccgtgg ttcttgcaac atgcttctca
     4741 acatcaatct ccaaaacctt tgtcttttca tatattttta aatcctgaat tatacttgag
     4801 ataaatttaa gaggatggaa ctgggcttgg ttgttgaact tgacagcaca ttctgtaggg
     4861 aagggaagag gtagttttcc ttcgatgtat tctgcatcga ttcccaggtc ttttgcaact
     4921 tctgcttctt ctttaattgc ttctgcatat tttggttccg tggtgtaaac atacgcagga
     4981 tttgtttcaa aatcgcattc tatattcaac ttgtcaacta tatatctgta agttttgaga
     5041 gcctcatcat tggcaatctt gtacattaaa gttttttctc tgccgatttg atttagaaaa
     5101 ttatgataac aaagaccgtg ttggatggtt acttttcctg tagttcttcc tgttgttccg
     5161 cttgcaattc tatcggattc tactatacag gcatcgactc ctttttgttt tagtaaaaag
     5221 gctgtcagaa tgccggataa accgccgccc attatcaaaa cgtcgcattc gatgtcttta
     5281 tccagggcat cgtattctgg cagtttgcat gtgtcaagcc ataaggacat aatatcactc
     5341 ctgtttattc atattaaagg tattatgtcc agactggtgg tggaatataa caaaagaggc
     5401 tgtttttttc taatcagtct tgctcaatac ttgtctcata cccatatagt tttccattaa
     5461 atcaaacaat ctatcttctg ttgttttcaa ttttggttta tgttgttttt ttaatctctc
     5521 cataattcct ttgataaacg taaaaagcat gat
//
LOCUS       DAONVY010000093         5518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10845_length_5518_cov_4.728171, whole genome shotgun sequence.
ACCESSION   DAONVY010000093 DAONVY010000000
VERSION     DAONVY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10845_length_5518_cov_4.728171"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4746
                     /locus_tag="PLI11_03695"
     CDS             <1..4746
                     /locus_tag="PLI11_03695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPZ51999.1"
                     /translation="NKICDIGAVRGDGAILHTKAVKELREFVAGSKYVCGHNIVRHDL
                     LYLYDVFDYDIMPIDTLLLSPLLFPQKPYHSLVKNDKIVSDDLNNPVNDCKQARTLFY
                     DEINAFNSLDSNLKTIYFGLLGKTKDFNGFFKYLGFNGICVNLEKLIRHEFEGLICAN
                     SDVQNYIKMHPIDLAYALALIGTKDPYSISPGWINYEFSMIENILHYLTNKPCNKGCP
                     YCNDKLDIHKALSRIFGFDSFRIYDGEPLQEKAVKAAVEGKSLLAVFPTGGGKSLTFQ
                     LPALIAGENSRGLTVVISPLQSLMKDQVDNLINKGITSVATINGLLNPIERANAIERV
                     YNGNAKILYISPEQLRSRTIEKMLLSRNVVRFVIDEAHCFSAWGQDFRVDYLYIGDFI
                     KQLQEKKSNNKTIAVSCFTATAKQKVIADICDYFESKLGLKLEIFATTSTRKNLHYRV
                     LFKESEGDKFNTLRELIIQKNCPTIVYVSRTKDAENLAKKLTASGINALPYHGKMETN
                     DRIKNQDMFINNEVQVIVATSAFGMGVDKKDVRLVVHFDISDSLENYVQEAGRAGRDP
                     NLQAECYVLYNDNDLDKHFVLINQTKLTMSEIQQVWQAIKRMTKNKPHVCCSALEIAK
                     QAGWDEEDYPVETRVKAAISALENAGYVKRGYNSPRCYANSILARNMAEASSRIDKSS
                     LFDEKGKQIAKRIMSFLISSKRRADAGTDEAESRVDYIADRLGLAKEVIIDSINIMRL
                     EGILADEQDMSALILDSKQKSSRILEEFFELELFLLAQLETEGRAINLKKVNDKAHDR
                     GVTHSNVKKIRKILDFLSLKNYIEKQEHMKTSYVEVKFNEEISKMNMSFHIRIDICRF
                     IVDELYSMLEQGKLVHFSLVSLFNSYREIASLDVNKKKISLDQVKEALFYLEIIGAIK
                     LEGGFLVHYQGMEIERLARGGKVRYKKEDYRMLDRFYEQKIQQVHIVGEYANLMVRDY
                     DAALRYVQDYFQMDFNKFIDKYFKGEKKKALEYNMTPKRYKKIFGQLSDKQKEILNDK
                     DSKYIVVAAGPGSGKTRVLVHKLASLLILEDVKHEQLLMLTFSRAAATEFKKRLADLI
                     GDAAYYVDIKTFHSFCFDLLGRTGNLDESDEVVKKAVKMIRNGEAELGKITRTVLVID
                     EAQDMDKDEYSLIRELIKYNEDMRVVAVGDDDQNIYEFRGSSSEYFKSLLTEYDAGRY
                     EMNENFRSKANIVALTNAFAETMKNRLKKDPIVPVQKENGIVRIINHTSMNIEEAVVN
                     NFVKEYKGGTACIMTNTNEESLRILWHLDRHNIQAKLIQSNDGFKMYNLVEVRYFLTQ
                     IDKELSSPVISDELWNKNKEKTLKKYARSSCINIIKNFFGEFEKVNKEKYRSDLEEFC
                     RESNYEDFYDENDDKIYVSTIHKSKGREFDEVYIMLSNIYPSDDEQKRKLYVGITRAK
                     EALYIHCNTNIFKNCSLPGVEIVYDNFEYGETEEIILQLTHRDVNLGFTKLKNKVLFN
                     IMSGDLLSLDNGYLTAEFNGRTHKVVMFSKKFKKTLNEYFEKGYVFDRAEARFLVSWR
                     DKEDNKEYPLVLPNIYMKKVEAIEKISTF"
     gene            4965..>5518
                     /locus_tag="PLI11_03700"
     CDS             4965..>5518
                     /locus_tag="PLI11_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52000.1"
                     /translation="MVTRGTGLLCMDKDSTVVEEGNVFLILPNVEHRYMIDNNLIVFH
                     MLLHSNFISRYSKELNSLSISLQNNLNITLDKSELDEIMNLLIELDQLQRSSDTSSDI
                     MKNSLSLYLICRLSRHMPEKDKNDEKTYPEGIVKCVEYMNLNYKESINMKKICSMLGV
                     SYSTLRRYFMGVYEMTPSDYLREL"
BASE COUNT         1891 a          898 c         1192 g         1537 t
ORIGIN      
        1 aataaaatat gtgatatagg tgctgtgcga ggagatggag caatactgca tacaaaagca
       61 gtgaaggaac ttcgtgagtt tgtagcagga tcaaaatacg tttgtggtca taatattgta
      121 cgtcacgatc ttttgtattt gtatgatgtt tttgattatg atattatgcc catagatact
      181 ttgcttctgt cgccgcttct ttttccccaa aaaccttacc attcccttgt aaagaatgac
      241 aagattgttt ccgatgattt gaataatcct gtaaatgact gcaagcaggc aagaactctt
      301 ttttatgatg agataaatgc attcaattct cttgattcca atctaaaaac catatatttc
      361 ggtttgcttg gaaagaccaa ggatttcaat ggtttcttca aatacttggg tttcaatggg
      421 atatgcgtta atctggaaaa acttatacgt catgagtttg aaggattaat atgtgcaaat
      481 tccgatgtac agaattacat aaaaatgcat cccatagacc ttgcgtatgc acttgccttg
      541 attggtacaa aggatcctta ttcaatttca cctggctgga tcaattatga gttcagcatg
      601 atcgaaaata ttcttcacta tcttaccaac aaaccctgca ataaaggatg tccatattgt
      661 aatgacaaac tagatattca caaggcatta agcaggatat tcggatttga ttcatttcga
      721 atatatgatg gtgaaccgct gcaggaaaaa gcggtaaagg cagctgtaga aggaaagtca
      781 ctacttgcag tttttccgac aggaggcgga aagtccctta catttcaact gcctgctctt
      841 atagcagggg agaattcaag gggacttact gtcgttattt ctccactcca atcccttatg
      901 aaggaccagg tggataattt gataaataag ggtattacca gcgtagccac tataaatggg
      961 cttttgaatc ctattgaaag agccaatgcc attgaaagag tgtacaacgg taatgcaaaa
     1021 attttatata tttctcctga acagcttcgc tcccgaacca ttgagaaaat gctgttgtct
     1081 agaaatgttg ttcgctttgt aatagatgag gctcactgtt tttctgcatg gggtcaggac
     1141 tttagagtgg actatctcta tatcggtgat tttattaaac aactacagga gaaaaaaagc
     1201 aacaacaaaa caatagctgt ttcctgtttt actgcaactg ccaagcaaaa agtaatagct
     1261 gacatatgtg actactttga atccaaactg gggcttaagc ttgagatctt tgctacaact
     1321 tccacacgta aaaaccttca ttacagggtg ttgttcaagg aatcggaagg agacaagttt
     1381 aataccttaa gagaactcat aattcagaaa aactgcccaa ccattgttta tgtatcaaga
     1441 accaaagatg cggaaaacct ggctaaaaaa ttgacggcat ccggaataaa tgcactacct
     1501 tatcatggca agatggaaac gaatgataga ataaaaaacc aggatatgtt catcaataac
     1561 gaggtgcagg ttattgtagc aacctctgct tttggaatgg gagttgacaa gaaggatgtc
     1621 aggcttgttg tgcattttga catatcagac tcgcttgaaa actatgtcca ggaagcaggc
     1681 agagcaggca gggacccaaa tcttcaagca gaatgctatg tgctttataa cgacaacgac
     1741 ttggacaagc attttgtgtt gattaatcaa acaaaactaa ccatgagcga aatacagcag
     1801 gtttggcaag ccataaaaag aatgacaaag aataaaccac atgtctgctg ttccgctctt
     1861 gagatcgcaa aacaggcagg atgggacgaa gaagattatc ctgttgagac cagggtaaag
     1921 gctgccattt ccgcattgga aaacgcagga tatgtaaaga gaggctacaa ctctccaaga
     1981 tgttatgcca acagtattct tgcaaggaac atggcagaag cctcaagtcg aatcgataaa
     2041 tcaagtttgt ttgatgaaaa aggaaagcag attgccaaac gcataatgag ttttttgatt
     2101 tcaagcaaaa gaagggctga tgcaggcact gatgaggcag aatcaagagt agattatatt
     2161 gcagaccgac tcggtttggc gaaagaagtt attatagatt caataaacat catgaggctg
     2221 gagggtattc ttgctgatga acaggatatg tcggccctta ttcttgattc aaagcagaag
     2281 tcatcacgaa tactagaaga gttttttgaa cttgaattat ttttacttgc ccagcttgaa
     2341 actgaaggca gagcaattaa ccttaagaag gtaaatgaca aagcccatga cagaggagtt
     2401 actcattcaa atgtcaagaa aattagaaaa atacttgatt ttcttagtct taaaaattat
     2461 atagaaaaac aggagcatat gaaaaccagt tatgtggaag taaagtttaa cgaggaaata
     2521 tcaaagatga atatgagctt ccacataagg attgacatat gccgatttat agtcgatgag
     2581 ttatattcca tgctagagca gggaaagctt gtgcatttct cgcttgtcag cctgtttaac
     2641 tcatatagag aaatagccag cctcgatgta aacaagaaga agatttctct agatcaggtg
     2701 aaagaagcgc tgttttatct tgagataata ggagcgatta agttagaagg cggttttttg
     2761 gtgcattatc agggcatgga aatagagaga ttggcaagag gaggaaaagt cagatacaaa
     2821 aaggaagact atagaatgct ggacaggttc tatgagcaga aaatccagca ggtccacata
     2881 gtgggagagt atgcaaacct tatggtcaga gattatgatg cagctctaag gtatgtacag
     2941 gattactttc aaatggattt taataagttt atagacaagt attttaaagg tgagaagaag
     3001 aaagccttgg aatacaatat gactccgaaa agatacaaaa agatatttgg gcagctatct
     3061 gacaagcaaa aagagattct taacgataag gattcaaaat atatagttgt ggctgcaggg
     3121 cctggcagcg gcaaaacccg cgttcttgta cacaaactgg cgtcactgct tattctggaa
     3181 gatgtaaagc acgaacagct cttgatgctt actttttccc gcgctgcagc aacagagttt
     3241 aagaagcggc ttgcagattt gataggagac gctgcatatt atgtggatat aaagactttt
     3301 cattcgtttt gctttgactt gttgggaaga acgggcaatt tggatgaatc tgatgaagtg
     3361 gttaagaaag ctgtcaaaat gataaggaat ggtgaagcgg agctgggcaa gataaccagg
     3421 acggttctgg ttattgatga agcacaggat atggacaagg atgagtatag cctgataagg
     3481 gaactaataa aatacaatga agatatgcgt gttgtagcag taggtgacga tgatcaaaac
     3541 atatatgaat tcagaggttc ttcttccgag tatttcaaat cactgttaac tgaatatgat
     3601 gcaggcagat atgagatgaa tgaaaacttt agatcaaaag ccaacattgt tgctttgaca
     3661 aatgcttttg ctgagaccat gaagaacaga ttaaaaaagg atcccatagt acccgttcaa
     3721 aaagaaaacg gcatcgtaag gatcataaat cataccagca tgaatattga agaagcagta
     3781 gtaaacaatt ttgtgaaaga atataaaggc ggaaccgcct gtattatgac gaataccaat
     3841 gaggagtctt tgcgtatctt gtggcatctt gacaggcaca atatacaggc caagcttatc
     3901 caatcgaatg acggctttaa aatgtacaat ttggtggaag tcagatattt tcttacccag
     3961 atagacaaag aactgtcttc accggttata agtgatgagc tgtggaacaa gaataaagaa
     4021 aaaacgttga aaaaatatgc cagaagcagc tgcatcaata ttataaaaaa cttttttgga
     4081 gagtttgaga aagtcaacaa agaaaagtac agatcagatc ttgaggagtt ttgcagagag
     4141 tcgaattacg aagactttta tgatgaaaac gacgacaaga tatatgtttc aaccatacac
     4201 aagtcaaaag gtcgtgagtt tgacgaagta tacatcatgc tttccaacat atatccaagt
     4261 gatgatgagc agaagcgtaa attgtacgtt ggtattacac gagcaaaaga agccctctat
     4321 attcactgca atacgaacat ctttaagaac tgctcgttac ccggagttga aattgtttac
     4381 gacaatttcg agtacggaga gacggaagag attatacttc agctaactca cagagatgtc
     4441 aatttggggt ttaccaagtt gaaaaataaa gtgttgttta acattatgag cggtgacttg
     4501 ctttctcttg ataatggtta ccttactgct gagtttaatg gcaggactca taaagtagtt
     4561 atgttctcta agaagtttaa gaaaacactt aatgagtatt ttgaaaaggg ttatgttttt
     4621 gatagagcgg aagctaggtt tcttgtatca tggcgggata aggaggataa caaggaatat
     4681 cctcttgttc ttccaaacat ttacatgaag aaggtggaag caattgagaa aatcagcaca
     4741 ttctaaattg actttgtgat tttgagatgg taacatttag ttatatttat acatatgtgg
     4801 gagatgttta aatgcggcag agatcggaac ttcacggtat gaaaacgggg gaacatttgg
     4861 atatattcaa caacaaaccc tttaaccagg taaaatgtta ttttcatgat acgtattata
     4921 tgggtgctat aaacatgcac aaacacaatt tttatgaact taacatggtt acaaggggaa
     4981 ctgggctgct ctgtatggac aaagatagta ctgtagtgga agaagggaat gtgttcctta
     5041 tactcccgaa tgttgaacat aggtacatga ttgataacaa cctgattgta tttcatatgc
     5101 tcctgcacag caatttcatt tcaagatact caaaggaact taattcgtta tccatctcat
     5161 tgcaaaataa tctgaacata accttagata aaagcgaact agacgaaatt atgaatttgt
     5221 taattgaact ggatcagctt caaaggtctt ctgacacttc ttccgacatc atgaaaaatt
     5281 ccctttcttt gtaccttata tgcagactat cccgacacat gcctgaaaaa gataagaatg
     5341 atgaaaaaac atatcctgaa ggaatagtaa aatgtgtgga gtacatgaat ctcaattaca
     5401 aggaaagtat aaatatgaaa aagatatgca gcatgctggg tgtttcgtac tcaacattgc
     5461 gaagatattt catgggagtg tacgaaatga caccgtcgga ttatctaagg gaattaag
//
LOCUS       DAONVY010000094         5514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10858_length_5514_cov_3.453563, whole genome shotgun sequence.
ACCESSION   DAONVY010000094 DAONVY010000000
VERSION     DAONVY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_5514_cov_3.453563"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLI11_03705"
     CDS             <1..1107
                     /locus_tag="PLI11_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52001.1"
                     /translation="DPNGIIYFGITVVRRRDEFKGKEDFAVLSYNPDTEELKFHGTFM
                     NAAKEAGTLHEDEQLPKGHTQFIYYNGKMHMGSQNFHDAGKFATKTTIPDNKPIDTMH
                     GGHLFSLDIETGELKDETAHLPNGVLIEHEGILQIRLIPGTSTLVCMSTPEYKLIFYN
                     LETRAIEKTVDTTGWMPNNPLSREIVIVEDRIYVYRGVEDDVTVRENLYPIYYYDITK
                     DEFVETEYEAFGGFWNGQCTTPDGKTTFVSSCCGELYSIDHETFEIKHHGNLKTPDKK
                     TVINYLYSLCMAPDGRKIFYVPSVYLKDYGVMQYDIETGEITSLSPMPLAVYTGNGVI
                     DDQGRLYFTKFGSASMRSGKFELCEITIQGSPIS"
     gene            1173..1493
                     /locus_tag="PLI11_03710"
     CDS             1173..1493
                     /locus_tag="PLI11_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HPZ52002.1"
                     /translation="MSKKEELKKKHVNMQLIKTRSSKITDMPVEKCNVEYWPACCSGL
                     CTYVGDASLKELKEADLLDEFLMKCQILSWDLKDKLEKGQITSHLYQCSLCGKYYMSM
                     DAFN"
     gene            complement(1490..1915)
                     /locus_tag="PLI11_03715"
     CDS             complement(1490..1915)
                     /locus_tag="PLI11_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPZ52003.1"
                     /translation="MMDSDRLKRFLRELKKKEVRMTCGLLGVECVSMSYVWDSFFDLK
                     KPYKKGARYTLDELLFMSDAEFEEVVDEYFFEIVDRLNYHVQHVLHPVLKLPINSDME
                     TVKKKFRELAKEMHPDTGGSIEEFLALYEAYELFKRKRY"
     gene            complement(1919..3853)
                     /locus_tag="PLI11_03720"
     CDS             complement(1919..3853)
                     /locus_tag="PLI11_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52004.1"
                     /translation="MKSLAVITDEKSDLSNVIEEIGYKTGVYTVEYINKRELGEKDLK
                     NFDCYCILGGTSEKPFSMNASSRIVLEKEREKGKKFFVEYCLSIGDVYSAEPVKTRFS
                     RLVYLGEDLNDTLFYEDILDDQCNDFSHTYFKGAESSPILVYKRYINSHSKASLNDDE
                     KNDITKWALWKYDDNTLVCNFRLCNFIKARFSPMKKWQSLVLWLVSWLCDNPLNVDYA
                     FETAYTNRSDMEIKDFDEAAKESFLMGMDWFHNANILLSGGKDGVLEGFHHDIDAKGV
                     QKVADTVRADCAGETSGAFFVHHMLTGDVKSLEIANNLEDYCYNNMQIKEGMYKGMIR
                     WTEVAWGVCYQDDVARVIMPTLLRSFYTKGSDRDTKHLDDVVEALWFLVKTTGTDGLR
                     PGRTDLLYLSDEEMNRIKNNPANFPSAHYNAFYHATLLMAYKLTGIEEFKEVAVRGLT
                     TLMTKYPDLYREQSETQEMCRLVLPLAWLYFVTGEEKHKKWLYRVCEDLKKLRHSFGS
                     YIEWDTGYRAARSRKIDSECSLLTKNGDPVSDLLYSVNWLPLAYIQSYLITKDEMFYD
                     MWKDITTFFIKSQILSKDSNLNGAWARGFDLEMHEVYGVPHDVGWGPWAIESGWTVAE
                     ILMGIGYGLMVDEIKQYYNE"
     gene            complement(3953..5326)
                     /locus_tag="PLI11_03725"
     CDS             complement(3953..5326)
                     /locus_tag="PLI11_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52005.1"
                     /translation="MRRKAFAKVLCLLLMLPVLGIMANAGATRYMGVTLYRSDYDYAP
                     SVMIGDGPLIKMWWGGGTGSGDGIYYSRLTPTGWESPRLVFTRSSSGWDSRHVCDPSV
                     IKGSFTFNGVNYSYAMYYTATYDNIGYDSHIGVAFSNDGVNWVRYGPPIISPHGDATP
                     PTKQYGAGMCTVYNDNGTIIMIYFDSTLGGNYMVTSTDGINFGNRQKVPHPRSWEHIG
                     DIAYSPSEGKWYICTKHGNDQEIYIYETENSSLMSTWRHTGGISSLTTGNVKNHNPGF
                     LRDPYGNIYFESNTEYIYVYYGTGSTSPSTWNIGQSIYVRGWEFEVEGNREGWTTKNL
                     MYDYGPSSGGSWVVYTHMLDPYFESPAIELPAENNRYIKVNIANQNTRTDGKIYFKTA
                     QENYYDESKTVTFTCTNGGGWWTHTIDMSVNPKYTGTITGIRIDPVSEGNGAPLGIAF
                     VRFSSTA"
     gene            complement(5401..>5514)
                     /locus_tag="PLI11_03730"
     CDS             complement(5401..>5514)
                     /locus_tag="PLI11_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HPZ52006.1"
                     /translation="VYAEYFKNKPARACVEVARLPKDVMVEIEAIAYIPEK"
BASE COUNT         1573 a         1261 c         1056 g         1624 t
ORIGIN      
        1 gatccaaacg gcatcatcta ttttggaatt accgttgtcc gccgaaggga tgaattcaaa
       61 ggcaaggaag actttgccgt attgtcgtac aatcccgaca cggaagaact caagttccac
      121 ggcactttca tgaatgccgc aaaggaagca ggcactctgc acgaagatga acagcttcca
      181 aaaggacaca cccagttcat ttactataac ggaaaaatgc atatgggctc gcagaacttc
      241 cacgatgcag gaaaatttgc tacaaaaacc accataccgg acaacaaacc catagacacg
      301 atgcatggcg gccatctctt ttcactggat attgaaacag gagaattgaa ggatgaaacc
      361 gctcatcttc caaatggtgt tttgattgag catgaaggaa tcctgcaaat tcgcctcata
      421 cccggcactt ccacactggt atgcatgtct accccggagt acaagctgat attctacaac
      481 ctggaaacaa gggccattga aaaaaccgta gacacaaccg gctggatgcc aaacaatccg
      541 ttaagcagag agatagtgat tgtggaagac agaatatacg tctacagagg cgttgaggat
      601 gatgttactg tccgtgaaaa cctatatccc atttactact atgacattac gaaggatgag
      661 tttgtggaga ccgaatatga ggctttcgga ggtttctgga acggacagtg cacaactccg
      721 gatgggaaaa cgacctttgt atcctcctgc tgcggtgaac tctattccat agaccacgag
      781 acctttgaga taaaacacca cggaaacctg aaaacgcctg ataagaagac tgtcatcaac
      841 tatctgtaca gcctgtgcat ggctcctgac ggaagaaaaa tattttatgt gccttccgta
      901 tacttaaaag attatggagt tatgcagtat gatatagaga cgggtgaaat tacatccttg
      961 tcaccaatgc ctcttgccgt ctacactggc aatggtgtaa ttgacgatca aggaagactt
     1021 tacttcacca agtttggcag cgcttccatg cgatcaggta aatttgaact ttgtgaaata
     1081 acgattcaag gttccccgat ttcataaaac ttattcctcc caatatccat atttttcttc
     1141 catgtgctat aatgggtttg ggaggaatat tgatgagcaa aaaggaagag ctaaaaaaga
     1201 aacatgtaaa tatgcagcta atcaaaacaa gatcgtcgaa aattacagat atgcccgttg
     1261 aaaaatgtaa tgtggagtat tggcccgcat gctgttctgg tttgtgtaca tatgttggag
     1321 atgccagctt gaaagagctt aaagaagcag acttgctgga tgagttttta atgaaatgtc
     1381 agattctttc atgggatttg aaagacaaac ttgaaaaagg gcagataaca agccatctgt
     1441 accagtgctc attatgcgga aaatattata tgagcatgga tgcctttaat taataccttt
     1501 ttcttttgaa cagctcataa gcttcgtaaa gggcaagaaa ttcttcgatt gaaccaccag
     1561 tgtcagggtg catttctttt gcaagttccc tgaatttttt ctttactgtc tccatgtcgg
     1621 agtttatcgg tagttttaga acaggatgca gaacatgctg tacatggtag tttagtctgt
     1681 ctacaatctc aaagaagtat tcgtcaacaa cttcctcaaa ctctgcatca ctcataaaga
     1741 gaagttcatc aagggtgtat cttgcccctt ttttatacgg ctttttcagg tcaaaaaacg
     1801 aatcccacac ataagacatc gaaacacact cgacacctaa aagaccgcaa gtcattctga
     1861 cttccttctt tttcaactcc ctgagaaatc ttttcaatct gtctgaatcc atcataattc
     1921 actcattgta atattgcttg atttcatcaa ccataagacc gtaacctatt cccatcaaaa
     1981 tctcagctac agtccagcct gattcaatcg cccaaggacc ccagcccacg tcatggggaa
     2041 ctccataaac ctcatgcatt tcaagatcaa atcctcttgc ccatgcacca ttcaggttag
     2101 agtctttgct gagtatttgg gatttgataa aaaacgttgt aatatccttc cacatgtcat
     2161 aaaacatttc atcttttgtt atcagataac tctgtatgta tgcaagaggc agccaattga
     2221 cagagtaaag aagatctgac actgggtctc cgttcttcgt caacagagaa cactccgaat
     2281 caattttacg gcttctggca gcacgataac ccgtatccca ctcaatatat gagccaaagg
     2341 aatgacgcag tttttttaga tcctcacaaa ccctgtacag ccacttttta tgcttctcct
     2401 ctcctgtgac aaagtacagc caggcaagcg gaagaacaag acggcacatt tcctgggtct
     2461 cactttgctc tctgtacaag tcaggatatt tggtcataag ggtggtaagt cctctaactg
     2521 caacttcttt aaactcctct attccggtca acttgtatgc catgagaagg gttgcatggt
     2581 aaaaggcatt gtaatgggca gaagggaaat tggcaggatt gttctttatc ctgttcattt
     2641 cctcatcgct taaatacaga agatccgtcc tgccgggacg aagaccgtct gttcccgttg
     2701 tcttgacaag aaaccaaaga gcctccacca catcatccag atgttttgta tccctgtcag
     2761 aacctttggt gtaaaagctt ctcagcaatg tcggcattat aacccttgcc acatcatcct
     2821 gatagcatac accccaggca acctcggtcc accttatcat gcctttgtac ataccttcct
     2881 ttatctgcat gttgttgtag cagtagtctt ccaggttgtt cgcaatttca aggctcttga
     2941 catccccggt gagcatgtgg tgaacaaaaa aagctcctga ggtttcccct gcacaatccg
     3001 cccttacggt atctgcaact ttctgcacac cttttgcatc tatgtcgtgg tgaaatcctt
     3061 ccaggactcc gtcctttccc ccggaaagaa gtatattggc attatggaac cagtccatgc
     3121 ccataagaaa gctttctttt gcagcctcat caaagtcctt gatttccatg tcgcttctgt
     3181 tagtatatgc tgtttcaaaa gcataatcaa cattaagagg attatcacaa agccaagaca
     3241 caagccacaa aaccagcgac tgccacttct tcatgggaga gaatcttgct tttataaaat
     3301 tgcaaaggcg gaaattgcaa acaagggtgt tgtcatcata tttccacaaa gcccattttg
     3361 ttatgtcatt cttttcatca tcattcaatg atgcttttga atgtgagttt atgtaccttt
     3421 tataaacaag aatcggcgaa gattccgcac ctttgaaata cgtatgggaa aaatcattgc
     3481 actggtcatc caaaatatct tcataaaaca aagtatcatt taaatcttct cccaggtaaa
     3541 caagtctgct gaacctggtt ttaacaggtt cagcagaata cacatcccct atactaagac
     3601 aatactctac aaaaaacttc ttgcctttct ccctttcctt ttcaagaact attctggaag
     3661 atgcattcat ggaaaaaggc ttctcactgg taccaccaag gatgcagtag cagtcaaaat
     3721 tttttaagtc cttttccccc aattcccttt tatttatata ttctacagta tatactcctg
     3781 ttttatatcc aatctcttca ataacattcg agaggtcact tttctcgtca gttattacag
     3841 caagactctt cataaattgt attctccttt catgcacacg tttctcattt tgcatagaag
     3901 gattatacat tcttgactac ccatttacaa gttaattttt atttaaattt ctttatgcag
     3961 tgctggaaaa tctaacaaaa gcaataccca aaggagcacc atttccttca gagaccggat
     4021 ctatcctgat accggtaatt gttcctgtat actttggatt aaccgacata tcaattgtat
     4081 gggtccacca tccaccgcca ttggtgcatg taaaggttac tgttttgctc tcgtcgtaat
     4141 agttttcctg agcggtcttg aaatatattt tgccgtccgt tcttgtgttt tggtttgcaa
     4201 tgttaacctt tatgtatctg ttgttttctg caggcaattc aatggcaggt gattcaaaat
     4261 atgggtcaag catgtgagta taaacaaccc atgatccacc ggaacttggt ccatagtcat
     4321 acatcagatt ctttgtggtc catccttccc tgttgccttc cacttcgaac tcccagccgc
     4381 gaacatatat tgattggcct atgttccagg tggaaggaga cgtgcttcct gttccatagt
     4441 acacatatat atactccgtg tttgattcaa agtagatgtt tccataaggg tctctcagga
     4501 aacccggatt gtgatttttc acattgcccg tggtgaggga tgaaataccg ccggtatgac
     4561 gccaggtgga cataagagag ctgttttcgg tctcatagat atagatttcc tgatcattgc
     4621 cgtgtttggt gcagatgtac cattttcctt cactgggaga gtatgcaatg tcaccaatat
     4681 gctcccagga tctgggatga ggcacttttt gtctgttgcc aaaattaatg ccgtccgttg
     4741 atgtcaccat gtagtttccg cccaatgtgg aatcgaaata tatcattatt atggtgccgt
     4801 tgtcattgta tacggtgcac atgcctgcgc catattgctt ggttggaggt gttgcatctc
     4861 catgtgggga aatgattgga ggaccgtatc tcacccagtt gactccgtca tttgagaagg
     4921 ccacaccaat atgactgtca tatccgatgt tgtcatatgt agcagtgtag tacatggcat
     4981 atgaatagtt tacaccatta aaggtaaaag aacctttgat aacacttggg tcgcatacat
     5041 gtcttgaatc ccatcctgac gaggaccttg taaataccag cctaggactc tcccatccgg
     5101 taggagtaag tcttgagtag taaataccgt ctccactacc ggttcctcct ccccaccaca
     5161 ttttgataag aggaccatct ccaatcatga cagagggcgc atagtcgtaa tcgcttcggt
     5221 aaagggttac acccatatac cttgtggctc ctgcatttgc cattatgccc aacacaggga
     5281 gcataagcag cagacacaat acttttgcaa aagcttttct tctcattctc tctactccct
     5341 tcatacagta ttcatactat tctatgcttt ttggcataaa aaaatctccc atagggagac
     5401 ttatttttct ggaatatatg ctattgcctc aatttccacc ataacatcct ttggaagacg
     5461 tgcaacctca acacaggctc gtgcaggttt atttttgaaa tactctgcat acac
//
LOCUS       DAONVY010000095         5510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10867_length_5510_cov_3.793401, whole genome shotgun sequence.
ACCESSION   DAONVY010000095 DAONVY010000000
VERSION     DAONVY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10867_length_5510_cov_3.793401"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLI11_03735"
     CDS             <1..741
                     /locus_tag="PLI11_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPZ52007.1"
                     /translation="KLEIAVCAQHNNGGLKVDIYWQSNIKGIFPIGEAAGIYGVYRPG
                     GSALNSTQTGSVRCAEYIKAKRRIEDVEDYVKYAFDELLDSVKETINLGSAFLKQKED
                     GLNIYELDIKLKKRMSEKAGAIRRLDLLKEALNEAKEDLSLVVEKASGRKIDINMFFY
                     TYYNLISQICYISAMIAYIENGGTSRGSYLVVDNQGNEKTFMDRILVTSYLNGNIKNY
                     WEKVRPIPEHDTWFENVWRDYREGNLFD"
     gene            719..1732
                     /locus_tag="PLI11_03740"
     CDS             719..1732
                     /locus_tag="PLI11_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018931078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPZ52008.1"
                     /translation="MKGIYLTENAGVIDRVYSKETQEKIAALVDMSAGWANREKVLAN
                     KEAYADVDIVFSTWSCPKFNEELLDVFKNLKIVLYGAGSLKSVVSDAFWNRGVRVCSA
                     WGANAVPVAHFTVAQVLYSLKLGWYYMDSFKRNRKRGIQDNDVKRIVYGTYKSTVGII
                     SLGMIGRMVAQLLKPFDLNIIAYDPFVTQEQADKMGLDVKMCTLDEVFSQSDVVSLHT
                     PLLPETVGMIEGRHFEMMKPSTTFINTSRGAIIKENEMIEVLKKRPDLYACLDVTHPE
                     PPKEDSPLFDLPNVILTPHVAGSVGRECTRMGDYMLSELEKFLNGEKLDWEVDKKRFP
                     IMA"
     gene            1741..2538
                     /locus_tag="PLI11_03745"
     CDS             1741..2538
                     /locus_tag="PLI11_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPZ52009.1"
                     /translation="MIKLGLVSVTFRNKNVEEVIEIAKRADLEAIEWGGDIHVPPNDL
                     KNAKVVGEKTIKAGLACCSYGSYYRVGAYGDNSMNSFIPVLETAKALSAQNIRVWAGV
                     KGSEQTDSDTRKRIEEESFAIVEEAKKEGMTVSYECHGGTLTDTLPSYMELLENVKKI
                     AKNNGKDSIAYAYFQPYASMPMKDVYEYLEKLNPYLKNLHVFNWKVVDGKIHRLPMTS
                     DGDYWNDIFKKVRNMSDRTHYALMEFVKNDSDHQFLEDAKVLRDFLN"
     gene            complement(2493..3344)
                     /locus_tag="PLI11_03750"
     CDS             complement(2493..3344)
                     /locus_tag="PLI11_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52010.1"
                     /translation="MYEWSAIYRETYEAEEEDIIDKEHLHPNMHQIVFVTEGEMELST
                     KGKDYHAKKGSISFISNIEMHCVKVKAFPYKRYIVNMDESFIQNIQMSKELTSILNLR
                     TDNFEHCVNVVEIMDELDCFFSKLVKESMNKKDKNVPYIYENASSLLILVLITLFRAK
                     PEAFPMPTKDMDSIIYKVKEYIDNNYTEEITVNSLSEQFFISQSYLSQNFKQLTGYSP
                     KKYIMLCRIAAARSLLYKTDMPINDIAMYVGFNDTSNFIRYFKKETGETPFQFRKSLK
                     TLASSKN"
     gene            complement(3418..4140)
                     /locus_tag="PLI11_03755"
     CDS             complement(3418..4140)
                     /locus_tag="PLI11_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPZ52011.1"
                     /translation="MSFNIQHCRDYIMAKEHGGDPIDFDLMADAIKSQEPDIVVLNEV
                     RNLGPCPSYKNQTKILAKKAGFQYYMFGEAIRFGGLLPYGNAILSKYPIGEFRVVRIP
                     DPIVKDEDVYYESRSVIKCRIDIGKHRIHVLGTHIGLANSEKTNAVNTLVELIDDTDI
                     PCVLMGDFNMTPEDDKLRPIHERMIDTARYFEEPKLTFPSDEPRIKIDYIFVSKDLKV
                     VNADVPDVIASDHKMVICDVEL"
     gene            4382..5062
                     /gene="sfsA"
                     /locus_tag="PLI11_03760"
     CDS             4382..5062
                     /gene="sfsA"
                     /locus_tag="PLI11_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HPZ52012.1"
                     /translation="MKYENIRKSKFISRKDRFTAYVEICGEIVPCHIKNTGRLKEILL
                     PDTEVYVQEVDKPERKTKYDLIAARKGDRIINIDSMVPNWVIEENMEELFPQLIGYER
                     EKTYKNSRFDFYVQTKSDKIYIEIKGVTQEFDNIAMFPDAPTKRGIKHVEELIDAVKN
                     GHKAYIIFVVQMEEVSYFTPNEAIHKEFADALVKAHENGVNICAYDCIVKSDEITLNN
                     NVKVMLSY"
     gene            5084..>5510
                     /locus_tag="PLI11_03765"
     CDS             5084..>5510
                     /locus_tag="PLI11_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52013.1"
                     /translation="MNKKLVKCLVLLMLVAAFLVMASACKKQDKDPEPTATATATEEP
                     TPDKTDEQTPEPTEEPTPEPTEEPTPEPTEEPDPVIHIQDDFSDPEKSKNEWELQAFK
                     VENGKLIADFNAYALQARVGGKLSNFTLAMDVVFLEHKPD"
BASE COUNT         1802 a          999 c         1144 g         1565 t
ORIGIN      
        1 aagcttgaga ttgctgtctg cgcacagcat aacaacggag gacttaaggt agatatatat
       61 tggcagagca atattaaagg tatctttcct attggtgaag ctgcaggcat ttatggtgta
      121 tacaggccag gcgggtcggc tcttaattca acgcagacag gaagcgtaag atgtgctgaa
      181 tatataaaag caaaaagaag aattgaagac gtagaagatt atgtaaaata tgcttttgat
      241 gaactattgg actctgtaaa agaaaccatt aatcttggca gtgcattcct aaagcaaaag
      301 gaagatggtt taaatattta tgagcttgac ataaagctga aaaagaggat gagcgaaaaa
      361 gcgggggcca ttagaagatt ggatttactc aaagaagctt taaatgaagc aaaggaagat
      421 ctttctttgg ttgttgaaaa agcaagtgga cgaaaaattg atataaacat gtttttctat
      481 acttattaca accttattag ccaaatatgc tatataagtg caatgatagc atacatagaa
      541 aacggaggaa ccagcagagg ctcatacctt gttgtagaca atcaaggcaa tgaaaagacc
      601 tttatggaca gaatacttgt aacaagctac ttaaacggaa atataaaaaa ctactgggag
      661 aaagtgcgcc ccataccgga gcatgacacc tggtttgaaa atgtttggag ggattatcgt
      721 gaagggaatt tatttgactg aaaacgcagg cgtaatcgac agggtatatt caaaagaaac
      781 acaggagaaa atagctgccc ttgtagacat gtccgcagga tgggcaaata gggagaaagt
      841 gcttgcaaac aaagaagcat atgcggatgt tgatattgtt ttttccacct ggtcatgtcc
      901 aaaatttaat gaagaacttt tggatgtgtt taaaaatcta aaaattgttt tgtatggagc
      961 aggatcattg aagagcgttg tctctgatgc attttggaac agaggagtaa gggtttgcag
     1021 tgcctgggga gcaaatgccg tacccgttgc ccactttacc gtggcacaag tattatacag
     1081 cctgaaactg ggttggtatt acatggacag tttcaaacga aacagaaaaa gaggaattca
     1141 agacaatgat gtaaaaagga tagtctacgg cacatacaaa agcaccgtag gcattatctc
     1201 gcttggcatg ataggaagga tggttgcaca gcttctcaaa ccatttgacc ttaacataat
     1261 tgcatacgat ccgtttgtaa cccaggagca ggcagacaaa atgggtcttg atgttaaaat
     1321 gtgtactttg gatgaggtgt tcagccagag cgatgttgtg tcactgcaca ctccactgct
     1381 gcctgaaacg gtaggaatga ttgaaggaag acattttgaa atgatgaaac cttccaccac
     1441 ctttataaac acttccaggg gtgcaataat aaaggaaaat gaaatgattg aggttctaaa
     1501 aaaacgtcct gacttatatg catgtcttga tgtaacccat cctgaacctc caaaggagga
     1561 ttcaccattg tttgaccttc ccaacgtgat tcttactcct catgtggcag gctcggttgg
     1621 ccgagaatgt acaaggatgg gagactacat gctttcagaa ttagaaaaat tcttaaacgg
     1681 cgaaaagctt gactgggaag tcgataagaa aagatttcca attatggcgt gaggtgaaga
     1741 atgattaaac ttggactggt ttcagttact tttagaaaca agaatgtaga ggaagttata
     1801 gagattgcga agagggctga tttggaagcc attgaatggg ggggagacat tcatgttcct
     1861 cccaacgact tgaaaaatgc aaaagttgtt ggggaaaaga caataaaagc tggtcttgca
     1921 tgctgttcct acggctcata ttacagagta ggagcatatg gcgacaactc tatgaattct
     1981 ttcatacctg tacttgaaac ggcaaaagcc ttgtctgctc aaaatatcag ggtttgggca
     2041 ggtgtaaaag gaagcgaaca gaccgacagc gatacaagaa aaagaatcga agaagaatcc
     2101 tttgctattg ttgaagaggc gaaaaaggaa ggtatgacag tatcttatga atgtcacgga
     2161 ggcacactga cggatactct tccttcatac atggagcttc ttgaaaatgt taagaaaatt
     2221 gctaaaaaca atggtaaaga ttctatagct tacgcatatt ttcagcctta tgcatcaatg
     2281 ccaatgaaag atgtatatga gtatcttgag aaactcaatc cgtatctcaa gaacctgcat
     2341 gtattcaact ggaaggtagt agacggaaaa attcacagac tgcccatgac ttctgacggg
     2401 gattattgga atgacatatt taaaaaggtg cggaacatgt ctgacagaac ccattatgcg
     2461 ttaatggagt tcgttaaaaa tgattcagac catcaattct tggaagatgc caaagtttta
     2521 agagactttc taaactgaaa cggtgtttca cccgtttctt ttttaaagta tctgataaaa
     2581 ttgctggtgt cgttaaaacc tacgtacatg gcaatatcgt ttattggcat atctgtcttg
     2641 tacaaaagcg atcttgctgc agcgatcctg caaagcatta tgtacttctt cggactataa
     2701 ccggtcagct gtttgaaatt ctgactaaga taggactgac tgataaagaa ttgctctgac
     2761 aaactattta cagttatttc ttccgtataa ttattatcta tatactcctt aaccttgtat
     2821 attattgaat ccatatcctt cgtaggcatt ggaaaagctt caggttttgc ccggaacaag
     2881 gtaatcaaaa caagtatgag cagtgaagac gcattctcgt atatgtatgg aacattcttg
     2941 tcttttttat tcatgctttc ctttacaagc ttgctaaaaa aacaatcaag ctcgtccatt
     3001 atttccacaa cattgacaca atgctcaaaa ttatctgttc ttaagttaag aatgcttgta
     3061 agttccttgc tcatctgtat gttctgaata aaggattcat ccatgttaac tatgtatcgt
     3121 ttgtatggga aagcttttac ttttacacag tgcatttcaa tgttgctgat aaaggagatg
     3181 gaaccttttt tggcatggta gtcctttccc tttgtactca gctccatctc accttcggtt
     3241 acaaagacaa tctgatgcat gttgggatgc aaatgctcct tatctataat gtcttcttct
     3301 tccgcttcat acgtttccct atatatcgca gaccattcgt acattttttt cacctcacgc
     3361 acattttaca acaaaacgag tgatttatca acaaaaactt cgcttttgtc ggttaactca
     3421 cagctctaca tcgcatatca ccattttgtg gtcggaggca atgacgtcag gaacatctgc
     3481 attcacaact ttcaaatctt ttgatacaaa aatatagtct atctttattc ttggttcatc
     3541 ggagggaaag gtcagttttg gttcttcaaa gtaccttgca gtgtctatca tcctttcatg
     3601 tattggtctt aacttgtcat cttcgggtgt catgttgaaa tctcccatta acacgcaggg
     3661 aatgtctgtg tcgtcaatta gttctaccag tgtgttaacg gcatttgtct tttcactatt
     3721 tgcaagcccg atatgagtac ccaaaacatg tattctgtgt tttccgatat ctatcctgca
     3781 tttgataaca gatcttgatt catagtatac atcttcatcc tttacaattg gatccgggat
     3841 gcgtaccacg cggaactctc caatgggata cttcgacaaa atggcatttc cataaggcaa
     3901 aagtccacca aaccttattg cctctccaaa catatagtat tggaaacctg ccttttttgc
     3961 caatatttta gtctggtttt tgtatgaagg acaagggccc aaattcctaa cctcgtttag
     4021 aaccacaatg tccggctcct gcgactttat agcatccgcc atcaaatcaa agtcaatggg
     4081 atctccccca tgttcctttg ccatgatgta atccctgcag tgctgaatat taaatgacat
     4141 caggcgtact accacctgca acaacccctt acaatcgttt tctattggag caattataca
     4201 ttttcaacaa aatcatgtca aacagtttac gccagtttct ttaacatgca ggttatgact
     4261 ctgcaaaaca ttaatgcatt ggatgcattt ttattaattt taatcaaacc atctttattt
     4321 ttttcttctg atttgatatt attcatataa aagaacttta gaaaaagcaa taaggagatt
     4381 catgaaatac gaaaacatta gaaaaagtaa atttatttca aggaaagata gatttacagc
     4441 atatgtggag atatgtggtg agattgtacc ttgtcatata aaaaacaccg gaaggttgaa
     4501 ggaaatcctt ctgcctgata cagaagtata tgttcaagaa gttgacaagc cggagagaaa
     4561 gacaaagtat gatctcattg ctgcaagaaa aggtgacagg ataattaata tagatagcat
     4621 ggtcccaaat tgggtcatag aagagaacat ggaagagctg tttccccaat taattggata
     4681 tgaaagggaa aaaacatata aaaattcaag atttgacttc tatgttcaaa caaaaagtga
     4741 taaaatatat atagaaataa agggagttac acaagagttt gataacattg ccatgtttcc
     4801 agacgcacct actaaaagag gaataaagca tgttgaggag ttgattgatg cagtaaaaaa
     4861 cgggcacaaa gcatacataa tttttgttgt acagatggag gaagtttcat actttacacc
     4921 caacgaagca atacacaaag agtttgccga tgcccttgtg aaagcccatg aaaacggtgt
     4981 aaacatatgt gcctatgact gcattgtgaa atctgatgaa ataacgttga acaataatgt
     5041 gaaggtaatg ctttcatatt aatatatatc aggaggtttt aagatgaata aaaagttagt
     5101 caagtgttta gttctgctaa tgcttgtagc tgctttcttg gtaatggcaa gtgcctgcaa
     5161 aaagcaggac aaagatccgg aacccacagc gacagcaaca gcaacagagg aaccaacacc
     5221 agataaaacg gatgagcaaa ccccggaacc gacagaggaa cctactccgg aacctacaga
     5281 ggaaccaacg ccggaaccta cagaggagcc tgatcctgtg atccatatac aggacgactt
     5341 ttcagatccc gaaaagtcaa agaacgaatg ggagctgcag gccttcaagg tggaaaacgg
     5401 taaacttatt gcagatttta atgcttatgc actgcaagcc agggttggtg gaaagctttc
     5461 aaacttcacc ttggcaatgg atgtggtttt cctggagcat aagccagata
//
LOCUS       DAONVY010000096         5477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10947_length_5477_cov_3.775360, whole genome shotgun sequence.
ACCESSION   DAONVY010000096 DAONVY010000000
VERSION     DAONVY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10947_length_5477_cov_3.775360"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1375
                     /locus_tag="PLI11_03770"
     CDS             164..1375
                     /locus_tag="PLI11_03770"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPZ52014.1"
                     /translation="MKYVVVLGDGMADYPVKELGYKTPLQYANKPNMDKLASSGELGL
                     VNTVPLNMKIPGSDIANMSVLGYDPEKYYTGRSPLEAVSMNIDLGEDDLAVRCNLVTL
                     SAEENYEDKTMVDYSSDEITSEEAAELIKLVDEKLGSNTIRFHAGVSYRHCMVWKLKE
                     DSNLDLTPPHDISTKKIANYLPKTEQGKILLELMKESEKLLKDHPINKDRIKRGLNPA
                     NSIWLWGEGRKPYLDSFYNKYKLKGAVVSAVDLIKGIGLCAGLKPLYVKGATGNINTD
                     FSAKAQIAIDYLLNEGDFVYIHVEAPDECGHRHEIENKVRAIELIDEKVVGPILEQME
                     KQGDFKIMILPDHATPLSLRTHTHDPVPYIIYDSTNINKSSNLNYDEESAKKTNLIIE
                     KGHKLMDRFLK"
     gene            complement(1401..2081)
                     /locus_tag="PLI11_03775"
     CDS             complement(1401..2081)
                     /locus_tag="PLI11_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52015.1"
                     /translation="MKKTLIIAFEGIDGSGKGVQLKEASERLSRKGYRVGVLDFPVYD
                     SFFGKEIGIFLSGKEETTASNMDVKSMSLWYAMDRWLAFKNTDLSVYDIVLLNRSTLS
                     NAVYQSNRTCDEKKRDLIAWIHELEYGILEIPKPDLFIVFDVTTDNSRRNVSLKGHRD
                     YVGDSADVYEKNRELLDKVRQGYIEAAKHFDNVWLLDCTVDGRMRPIEEIAEEIERRI
                     ESLINQKR"
     gene            complement(2101..2967)
                     /locus_tag="PLI11_03780"
     CDS             complement(2101..2967)
                     /locus_tag="PLI11_03780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPZ52016.1"
                     /translation="MVTKFFKVFIALTMCFVLGSCTKANEEKKEDIIEPTEEPPVTET
                     LREPVSDFIKSLDKSKPHKFVTFSFDDGTVQDKRLAELFNKYGIKCTFNINTGSLGVE
                     GTLNLQDGNVDFTKLSAEEIKSVYEGHEVAVHTVNHPDLRTLEDEDIIKEVNDDFNTI
                     QEVMGQKVIGMAYPGGPFYNRRVIDVIQNNTEIKYARETSSTYRFTMPNNFMIWRPTC
                     HQSEVKNLVDKFISQEAKEDMLLYVWGHSWEFDLYDSWDEFESILKKLSEAEDLIFVT
                     NSEIYYYLNNGF"
     gene            complement(3046..4488)
                     /locus_tag="PLI11_03785"
     CDS             complement(3046..4488)
                     /locus_tag="PLI11_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPZ52017.1"
                     /translation="MKKVAIYVRESRDDLGLSYETIETQKSLLEKFVEEKNLGAVYKV
                     YEDDNYSGINFERPGIKKLIEDASFGKFDILVAKDLSRLGRSNARTLLFLESVEEYGV
                     RVLTYDGRYDSLRDAETVGIDSWFNERYVIDISRKIRANLRHKIERGEYIGNPPYGYV
                     KSKDVRNKLEVCEEEAFVVREIYKLYLEGYGYKKISEILEEKGIGKKKWNVTQIKRIL
                     TSRVYVGDTVQGVSERVSYKSKKTRRLPKSEWVITKNTHEPIIDEETFDKVQKERIKR
                     QKGSGNHKGGLSIFKGLVFCADCNKRMYSRKQTKGADGYTCQTYEKQGRAACSRHFVS
                     ESYLSNIVFSDIYMVLSDYVKSNPNCIEELAQINEERDEEINTLRNLIESSLRKQKIL
                     YYDRLDGRITADMYDSMNLELKSAIERYKDEIDRLERAVENKTESLNLLLKTLKSYKD
                     LSGEVKRKIVKIVVEKITVSESEVCIWYNL"
     gene            complement(4644..>5477)
                     /locus_tag="PLI11_03790"
     CDS             complement(4644..>5477)
                     /locus_tag="PLI11_03790"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPZ52018.1"
                     /translation="PVFTDAKIFTNDELGLGVEKVILTPSISAFIGGDITAGVLSTGM
                     YKSDKNILLIDMGTNGEMVLNNRGKLIGASTACGPAFEGANIKYGMGGVTGAICKVEM
                     KEDGALNVYTLGNEKARGICGSGLIDLIACLVNMELIDETGFMEEDFEVSPGIYLTTR
                     DVREFQNAKAAIGAGIRVIAKHAGISLEEIDEVLLAGGFGSYIDINSAIDATLLPYEF
                     RGKISSCGNSSGKGASQILLEESLLNAAIDISKAVDYIELANSKYFSDFYVMSMMFEQ
                     E"
BASE COUNT         1657 a         1287 c          854 g         1679 t
ORIGIN      
        1 tgtcgaatct ccaccacaag gaagaagctt tgctcttgac attgcaagaa cgtacggaat
       61 tacctttgaa caattgcttg attcacataa aaacagacat gaactgggtg tggaggaaga
      121 aactcctctc ccaagaagga tagtataggg tgaaatttaa tatatgaaat atgttgtagt
      181 attaggtgac ggaatggctg attacccagt gaaagagctg ggctataaga caccattgca
      241 atatgccaac aagccaaaca tggacaagct tgcttcatca ggtgaattgg gacttgtgaa
      301 taccgtgccc ttgaacatga agatacctgg cagcgatata gccaacatgt cggttttggg
      361 atacgatcct gaaaaatact atacaggacg ctcccctctt gaagctgtaa gtatgaatat
      421 cgatttagga gaagatgatc tggctgtacg atgcaatctg gtaacactca gcgctgaaga
      481 gaactatgag gacaaaacca tggttgacta cagcagtgac gagattacat cggaagaggc
      541 agccgaactt attaaactgg ttgacgaaaa gcttggcagc aataccatca gattccatgc
      601 cggagtcagc tacaggcact gcatggtttg gaaattgaaa gaagattcaa accttgacct
      661 gacacctccc catgacatat caacaaaaaa gattgccaat tacctgccca aaacagaaca
      721 aggaaaaatc cttcttgaac ttatgaagga aagcgaaaag cttcttaagg atcaccccat
      781 aaacaaagac agaataaaga ggggtttgaa tcctgcaaac tccatctggc tttggggcga
      841 agggagaaag ccttatcttg acagctttta taacaagtac aagttaaagg gagcggttgt
      901 atcagcagtt gacttaatca agggtatagg tttgtgtgca ggccttaagc cactttatgt
      961 aaaaggtgca acgggcaaca tcaacacgga cttttccgca aaagctcaaa ttgcaattga
     1021 ctaccttctc aatgaaggag actttgttta catacatgtg gaagccccgg atgaatgtgg
     1081 gcacaggcat gaaatcgaga acaaggtaag agcaattgag cttattgacg agaaagtggt
     1141 tggtccgata cttgagcaaa tggaaaaaca gggcgacttc aagattatga tactgcccga
     1201 ccatgcaact ccattgtcac taagaaccca cacccacgat ccggtgcctt acattatata
     1261 tgacagcacc aatataaata aatcgtcaaa tctcaattat gacgaggaaa gcgcaaagaa
     1321 aacgaacttg ataatagaaa aaggtcacaa attaatggac cgttttctaa aataacacca
     1381 aaaaagatag gattaatatc ctatcttttt tgatttatca aactctcaat tcttctttca
     1441 atttcctcag cgatctcctc tatcggtctc atcctgccat caacagtaca atcgagcagc
     1501 catacattgt caaaatgctt tgcagcttca atataccctt gtcttacctt gtcaagaagt
     1561 tccctgttct tctcatagac atcggcacta tcgcctacat agtccctgtg cccttttaag
     1621 gaaacattcc ttcttgaatt gtcagtagtt acatcaaaca caataaacaa gtcaggtttt
     1681 ggtatctcaa gaatcccata ctcaagctca tgaatccaag ctatcaaatc cctcttcttt
     1741 tcatcacaag ttctgttgct ttgatacacc gcattggaca aagtggatct gttaagaaga
     1801 acaatgtcat atacggacaa atccgtattt ttgaaagcaa gccatctatc catggcgtac
     1861 caaagggaca tggatttaac atccatgtta ctggcagtag tttcctcttt tccagaaaga
     1921 aaaatgccta tttcctttcc aaaaaaggag tcataaacag ggaaatccaa aacccctacc
     1981 ctatagcctt ttcttgaaag cctctcacta gcttccttta gctgcactcc ttttccactg
     2041 ccatcaatgc cttcaaaagc aatgattaag gtttttttca tttcactact ccttgccaaa
     2101 ttaaaatcca ttgttcaaat aatagtatat ctcactgttg gtgacaaata tcaggtcttc
     2161 cgcttcagac agttttttga gtatgctttc aaactcatcc caactgtcat ataaatcaaa
     2221 ctcccaggaa tgaccccata cataaagaag catatcctcc tttgcttcct gtgatatgaa
     2281 cttgtcaaca agattcttca cttcagactg gtgacaagta ggtctccata tcataaaatt
     2341 gttgggcatg gtaaaacgat aggttgaaga tgtttctctt gcatacttta tttccgtatt
     2401 attctgtatt acatcaataa ccctgcggtt gtaaaacggt cctccaggat aggccatgcc
     2461 tatcaccttt tgtcccataa cttcctgaat tgtattgaaa tcatcattca cttccttaat
     2521 tatatcctca tcttccaagg ttcttaaatc aggatgattt actgtatgca cggcaacttc
     2581 atgtccttca tacacacttt tgatttcctc ggctgatagc ttggtgaagt ccacattacc
     2641 gtcctgcaaa tttaaagtac cttccacccc tagtgaaccg gtatttatgt tgaatgtgca
     2701 cttaatacca tatttgttga acaactcagc cagccttttg tcctgaactg ttccgtcatc
     2761 aaaactaaaa gtcacaaact tatgcggctt gctcttgtcc aaactcttga taaaatcaga
     2821 cacgggttct ctcaaggttt ccgtcacagg tggctcttcc gtaggctcga taatatcttc
     2881 ttttttttct tcgttggctt tcgtacatga accaagcaca aaacacatag ttaatgcaat
     2941 aaaaactttg aaaaactttg taaccatata tcctccaaaa aaaagtaatc atcctctagt
     3001 ttagcaccag aaaaaacata aaacaagagg cacatgcccc ttttatcaca aattatacca
     3061 gatacaaacc tcactttcag agacagttat cttctcaacc acaatcttta ctatcttcct
     3121 ctttacttcc ccgcttaaat ccttatatga ttttaatgtt ttaagaagca aattaagaga
     3181 ttctgtttta ttctccacag cccgttccag tctatcaatt tcatccttgt acctttctat
     3241 tgcagactta agctcaaggt tcatactgtc gtacatatcc gcagtaatac gtccatcaag
     3301 tctgtcatag tacaatattt tctgttttct caaagaggat tcaataagat ttcttaatgt
     3361 atttatttcc tcatccctct cttcatttat ctgtgccagc tcttcaatac agttaggatt
     3421 actctttaca taatcactca gcaccatata aatatcggaa aaaacaatat tggataaata
     3481 actctcactt acaaaatgtc ttgaacaagc agccctgcct tgtttttcat aagtctgaca
     3541 tgtatatcca tccgctcctt ttgtctgttt tctggaatac atccttttat tgcagtcagc
     3601 acaaaataca agacctttga aaatacttaa tccacccttg tgatttccac tacctttttg
     3661 tcttttaatt ctttcctttt gaaccttgtc aaatgtttct tcgtcgataa ttggttcatg
     3721 ggtgtttttt gtaatcaccc actcggactt gggcaacctt cttgttttct tgcttttata
     3781 gctgactctt tcacttactc cctgcacggt atctcccaca tagacgcggc ttgtcaggat
     3841 tctttttatt tgcgttacgt tccatttctt cttgccaata cctttttcct caagtatttc
     3901 ggaaatcttc ttgtatccat aaccttcaag atacaactta tatatctccc taaccacaaa
     3961 agcttcctct tcacaaactt ccagcttgtt tctaacgtcc tttgatttta cataaccata
     4021 aggaggattc ccaatatatt ctcccctttc aatcttatgt ctcaaattcg ccctaatttt
     4081 tcttgatatg tcaattacat atctttcatt aaaccagctg tctataccta cagtctcagc
     4141 atccctcaaa gaatcatatc ttccgtcata agttagaacc cttactccat actcttccac
     4201 actttcaaga aaaagaagcg ttctggcatt gcttctccca agccttgaca agtctttagc
     4261 aacaagaatg tcaaactttc caaaggatgc atcttcaatt agtttcttaa tgccaggacg
     4321 ctcaaagttg atacccgagt aattgtcatc ctcatatact ttatatacag cgcctaaatt
     4381 tttttcttca acaaatttct ctaaaagact tttctgtgtt tcgatggttt cataagacaa
     4441 tccaaggtca tccctgctct cccttacata aatggctacc tttttcatta aacaatcacc
     4501 taaatgtcaa tatccacaat caaagacaaa agggcgagca gggattcctt cacacaacct
     4561 ttatagactc tctttatctc atatttcatc aagaatcatc cttgacaatt cgtttaatta
     4621 atatatatga gataataaga aagctattct tgttcaaaca tcatggacat gacataaaaa
     4681 tcactaaaat acttgctgtt ggcaagctca atgtagtcta cagccttgct tatatcaatt
     4741 gcagcattca aaagactttc ttcaagtaat atttgactgg ctccctttcc tgaagaattt
     4801 ccacatgaac ttatcttccc tctaaactca tatggaagaa gagttgcatc aattgcacta
     4861 tttatgtcaa tatagctgcc gaaacctccc gctaacagaa cttcatctat ctcctcaaga
     4921 gaaatgcctg catgttttgc aataacacgt ataccggcac ctatggcagc ctttgcattc
     4981 tgaaactccc ttacatccct tgtggtaagg tatatgccag gacttacttc aaagtcttcc
     5041 tccatgaaac cagtttcatc tatcagttcc atgtttacaa gacaagcaat caaatcaatc
     5101 agaccacttc cgcaaattcc tctagccttt tcattgccca gtgtatatac gttcaaagcc
     5161 ccatcctctt tcatctcaac cttgcagatg gctcctgtaa caccacccat cccgtacttt
     5221 atattcgctc cttcaaacgc cggaccacag gcggttgatg ctccaattaa tttacccctg
     5281 ttgttcaaaa ccatttcacc attcgtaccc atgtctataa gtaaaatatt tttgtcgctc
     5341 ttgtacatgc cagtagacag aacacctgcc gtaatatctc cacctataaa tgcggatatg
     5401 gagggtgtca aaatcacctt ttccactccc aaaccaagtt catcattggt gaatatcttt
     5461 gcatcggtaa acacagg
//
LOCUS       DAONVY010000097         5467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_10982_length_5467_cov_3.634516, whole genome shotgun sequence.
ACCESSION   DAONVY010000097 DAONVY010000000
VERSION     DAONVY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10982_length_5467_cov_3.634516"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PLI11_03795"
     CDS             complement(<1..1732)
                     /locus_tag="PLI11_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52019.1"
                     /translation="MDYFQTNEASNYSDAAKKRNRIILIIAAVAIGLVGFYAVVEFIS
                     SLDLFYTRRTISYNEMVYQRPDTDKIFDKIDEIIEVVNSKATFYKKLKAVKSLEKDYE
                     NFYTMSALAYVKHKSDTTDDFFKEEVEFFGEVTPIFQQKLEEMFIACSESSHAERFEL
                     EYFGEGFFDSYKDGSIYSDNFVKLLQEESRLGNEYIEAVSSATINYNGKEETVETILQ
                     MDLEEEEFDALMTMYYEKYNDILGEIYVDLVKTRLRIAQELGYESYMDYAYEYFGRDY
                     TAEQASLFMQDVKNELVPLYKKVKQDELFYIFEEVPNMDEKRVFEIVKKAAVDMSTKT
                     GKIFSFMEKYELYDISASPNKAPGSFQTYLRAYDAPFILMNLEGNAYDVLIFAHEFGH
                     FVDSYLNYDYTVSIEDAEVASQSMGYLILNYLNNCSEEEVSILSLMQFLKTLDLYIYQ
                     GYYNEFEERVYSLRENDVTLENINSIANQCAVSFGLYDDEWKDYYSLQWVDIIHFYEL
                     PFYVIGYCISNDAAIQIYEHELAKKGSGVDYYLDLMNSDWSLSYLESVEKAGLESPFA
                     EGRIAKVSSLL"
     gene            complement(1800..2501)
                     /locus_tag="PLI11_03800"
     CDS             complement(1800..2501)
                     /locus_tag="PLI11_03800"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPZ52020.1"
                     /translation="MKLLIIRHGDPDYKNDTLTPKGWIEADLLSKRLEKINISKIYCS
                     PLGRAKDTAKATLKIKNMDMEILDWLQEFPLPVKPDYEKEGLPVHHNGCHCPWDITPQ
                     YWDKYKDVFFSNDWMSHSVYKDSGIYEYYTKVGNELDKLLEKYGYIRKGQLYDVTEKA
                     RDDETIVFFCHMGLGLTLVSCLTRIPIPQIWHGFQLMPTSVTVVEMQRTPQLHDAAIA
                     RIVQMGDLSHLYNMD"
     gene            complement(2721..4379)
                     /locus_tag="PLI11_03805"
     CDS             complement(2721..4379)
                     /locus_tag="PLI11_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPZ52021.1"
                     /translation="MRRNNYIKKLCLLLLPVLLVSVFLYSCNKEFDPTKDYKPIIAEH
                     EDSTLKLTFVHSFTKVAQDEKPKGNNSYYIRLAKNEIEGCQFVLYSENDKENLTAEIT
                     EFKNEAGDAIPAELFIEHYISTGDAMTPDPIVPLSSEFNLEGSKSQAFYIKIKTTPDT
                     PAGIYEAMLSIKDGSKTIKQAKVFAKVWDFTLSEATACTTAMDLSHWAVYVKHEQWDS
                     DDNELYKIYYDYLLENRVNAYYLPYDLLDPRVDEYLDNPRVTSFCITGYKTDYNFDRL
                     RAIHEKLSQKEEWFDKGYFYFVDEPTDMHLLNEIEFHGMNLETVYPGYRMVAPFFTDI
                     AVDYKTDQIEFMSDYIRIWCNKPYAYKTAKENKKEPKYIVQSEKYDKQYGTYAERMEE
                     FKKRGDKVWWYVCWEPQDPYTNILMEQKGVVHRVLFWQQKQYNVDGFLYYAVNNWTEN
                     AWEDAITGKHLMPHVYGDGVLIYDGAHVGIRGPVGSLRLEAVRDGIEDFEYLTMAEKE
                     LGKKKTNKIIKKVSKSILDYTKNDETFVKARIELGNKLEKALKK"
     gene            complement(4485..>5467)
                     /locus_tag="PLI11_03810"
     CDS             complement(4485..>5467)
                     /locus_tag="PLI11_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPZ52022.1"
                     /translation="VIEVVGKKIYIKGSTEYGTYTGIKTFLEKFNNKGKIVVNKKTYE
                     KGDGLDMIISPIETELKIDGIKKDYIFVHVTDSHLTLFSDDDTKEVKASAYGRSQVFK
                     TADGIPSGDRFPAFFEYAEKVNADMIFLTGDIADFPTEANIKAIADNVSRSKVPSLYV
                     LGNHDWSFEFDYHSQSTKEKYIPKYKDLCGGNTDFQVKQFDDLQIIAINNSQDQVTYE
                     QYELTKKQLENGLPTIIIMHIPIYVDTLAQATIDVWGKPILMGPGGVSPTESTTLFYN
                     LITDENNNVQAIFAGHVHFDHEDEFLPGRMQIVTKNATDGHFRVVKISGK"
BASE COUNT         1557 a         1183 c          863 g         1864 t
ORIGIN      
        1 ccaataatga agaaacctta gctatacgcc cctcggcaaa ggggctttcc aaacctgcct
       61 tttctacgct ttccaaataa ctcaagctcc agtcactatt catcaagtcc aaatagtagt
      121 ctacaccgct accctttttg gccaattcat gctcatatat ctgtattgct gcatcgtttg
      181 aaatgcagta gccgatgacg taaaaaggca gttcgtaaaa atgaattata tcaacccatt
      241 gaagcgaata gtagtctttc cattcatcat catataatcc aaaacttaca gcgcactggt
      301 tggctatact atttatattc tcaagagtaa catcattctc tctgagacta tagaccctct
      361 cttcaaattc gttatagtat ccctggtata tgtataaatc aagggttttt aaaaactgca
      421 tcaacgagag aatactaact tcttcttccg agcaattgtt taagtaattt agaatcagat
      481 atcccatgct ttgggatgcc acctcagcat cctcaatact gacagtatag tcataattta
      541 aatatgagtc gacaaaatgg ccaaactcat gtgcaaaaat caatacatca tacgcatttc
      601 cttccagatt catcaggata aacggtgcgt catatgctct caaatacgtc tggaatgacc
      661 cgggtgcttt gtttggagat gcggatatgt catataactc gtacttctcc ataaagctga
      721 agatcttacc agttttagtc gacatgtcta ctgctgcttt ttttacaatc tcaaaaacac
      781 gcttctcatc catgtttggt acttcctcaa agatatagaa aagttcatct tgttttacct
      841 ttttatataa tggaaccaat tcatttttaa catcctgcat gaacaaactt gcctgctcgg
      901 cagtatagtc ccggccaaaa tattcataag cataatccat atatgattcg tacccaagtt
      961 cttgcgcaat tctcaggcga gtcttcacaa ggtcaacata tatttcaccc aggatgtcat
     1021 tgtatttttc ataatacatg gtcatcaagg catcaaattc ttcttcctcc aaatccatct
     1081 gcagtatggt ttcaacggtt tcttccttgc cattgtagtt aattgttgca ctgctcactg
     1141 cctctatgta ctcattacca aggcgagact cctcttgcag aagtttgaca aaattatcac
     1201 tgtaaatact tccatcttta tatgaatcaa aaaacccttc cccgaaatac tccagttcaa
     1261 acctctctgc atgactgctc tcactacagg caataaacat ttcttcgagt ttttgttgaa
     1321 atatgggggt aacctctcca aagaactcca cctcttcctt gaagaaatca tcagtagtat
     1381 cgcttttatg cttgacataa gccaaagctg acatagtata gaaattttca taatcctttt
     1441 ccaaagactt aactgcttta agcttcttat aaaatgtcgc tttgctgttc actacttcaa
     1501 ttatctcatc aatcttgtca aaaattttat ccgtgtcagg tctttgatat accatttcat
     1561 tgtatgatat ggtgcgtctt gtataaaaca agtccagtga acttatgaac tcgacaacag
     1621 cataaaaacc caccaatcca attgctacag cggcaatgat taaaataatt ctgttacgct
     1681 tctttgccgc atctgaataa ttgcttgctt cattcgtttg aaaataatcc attatgtacc
     1741 tcacgaaatt taattaccgt gtcaataata tcacgtcata aaccaattgt caaaatcttc
     1801 taatccatat tgtataaatg tgaaagatca cccatttgaa caatacgggc aattgcagca
     1861 tcgtgaagct gaggtgttct ctgcatttct acaactgtta ctgatgtggg catgagctga
     1921 aaaccatgcc atatttgagg tattggtatt cttgtaagac aggataccaa tgtcaaacca
     1981 agccccatat gacaaaagaa aacaattgtt tcatcatccc tggctttctc tgtaacatca
     2041 tacagctgcc ctttccttat gtaaccatac ttttcaagca gcttgtcaag ctcattacct
     2101 accttcgtat aatattcata gatgccgcta tccttataca cggaatgact catccagtca
     2161 ttggagaaaa acacatcctt gtatttgtcc cagtattggg gagttatatc ccaaggacaa
     2221 tgacagccat tatgatgtac aggaagacct tctttctcat agtcaggttt gacagggagt
     2281 ggaaactcct gcagccagtc gagtatctcc atatccatgt tttttatctt aagagtagcc
     2341 ttggcggtat ccttcgcacg acccaatggc gaacaatata tcttggatat attaatcttt
     2401 tcaagccttt tggataacaa atcagcttca atccaaccct ttggtgttag tgtatcgttc
     2461 ttgtaatccg gatctccgtg tctaataatc aacagcttca tatttttact tcctttccaa
     2521 ctagtatgtc atattgcact gctcctccaa ttatatcaat ccattctcat tttgcaatat
     2581 aaccaaacct tctgtccggt gcggtatcac cattgagaag cttcaataat gtagccggat
     2641 aaacttttat gcctaaaaga aagagagttg ctgcctttaa gaagcatgca actctctttg
     2701 tatcctgaca tttaaatttt ttactttttc aatgcttttt ccagcttatt tcccaactca
     2761 attcttgctt tcacaaatgt ttcgtcattc ttagtgtaat ccaatatact tttagatact
     2821 ttctttataa tcttattggt tttcttttta cccaactctt tttctgccat ggttaggtac
     2881 tcgaaatctt caatgccgtc acgaactgct tccagtctta acgaacctac aggacctcta
     2941 atacctacat gggcaccatc ataaataaga acaccatctc catatacatg aggcataaga
     3001 tgttttcctg ttattgcatc ttcccaagca ttctctgtcc agttatttac tgcatagtat
     3061 agaaatccat ctacattata ttgtttttgc tgccagaaaa gtactctgtg aactacgccc
     3121 ttttgctcca ttagtatgtt tgtataaggg tcttgcggtt cccagcatac gtaccaccaa
     3181 actttatctc ctcttttttt gaactcttcc attcgttcag catatgttcc atactgtttg
     3241 tcgtattttt cggactgaac gatatattta ggctctttct tgttctcttt agccgtcttg
     3301 tatgcatatg gtttattgca ccaaattctg atatagtctg acataaattc gatttgatcc
     3361 gttttgtagt caaccgctat gtctgtaaaa aatggagcca ccattcgata gcctggatat
     3421 accgtttcca ggttcatccc atgaaactca atttcattca gaagatgcat atcagtaggt
     3481 tcatctacaa aatagaagta tcctttatca aaccactctt ccttctggga tagtttttca
     3541 tgaatagctc tcaatctgtc aaaattgtaa tctgttttat acccagtaat gcaaaatgaa
     3601 gtcactctcg gattgtccaa gtattcatct actctcggat caagaagatc atatggaaga
     3661 taatatgcat ttactcgatt ctcaagcagg taatcataat atatcttgta aagctcatta
     3721 tcgtctgaat cccattgttc atgctttacg tatacagccc aatggctaag atccattgct
     3781 gttgtacatg ctgttgcttc acttagagtg aaatcccaca cttttgcaaa cacttttgct
     3841 tgcttgatgg tcttgcttcc atccttaata gacaacattg cttcgtaaat gcctgctggt
     3901 gtatcaggtg tggtctttat tttaatatag aaggcttgcg atttagaccc ttctaaatta
     3961 aactcagaag acagcggcac aataggatca ggcgtcattg catctcccgt actgatataa
     4021 tgttcaatga atagttcagc gggaattgca tctcctgctt catttttaaa ttcagtaatt
     4081 tctgctgtca ggttttcctt atcattttct gaatagagaa cgaactgaca accctcaatc
     4141 tcgttctttg caagacgtat gtaatatgaa ttatttcctt tgggcttctc atcttgcgct
     4201 acttttgtaa aagaatgtac aaatgtaagt ttaagtgtac tatcttcatg ttctgcaatg
     4261 attggcttat aatcctttgt tggatcaaac tctttgttgc aggaatacaa gaaaactgat
     4321 accagcaaaa ctggcaacaa gagtaaacat aacttcttta tgtagttgtt cctacgcata
     4381 aaataactcc ttttgtatat tatagcttac tatagattat attcattgta ctaaaaaata
     4441 caagatgttt ttataaagaa gtcctcgcgc agcgaggact ttcattactt gccagatatt
     4501 tttacaactc taaaatgtcc gtctgtagca ttttttgtta caatttgcat tctgccaggc
     4561 aaaaactcgt cttcgtggtc gaaatgcaca tgacctgcaa atatagcttg aacgttattg
     4621 ttctcatctg ttatcaggtt gtagaaaagg gtggttgatt ctgtcggact tactccacca
     4681 ggtcccatca ggatgggttt tccccagaca tctattgtgg cttgagccaa cgtatcgaca
     4741 tatatgggga tatgcatgat tattatcgtt ggcaaaccat tttcaagctg ttttttggta
     4801 agttcatact gctcatatgt tacttgatcc tgactgttgt taatagcgat tatctgaaga
     4861 tcatcaaact gtttaacttg aaaatccgta ttgcctccgc acaaatcctt gtatttagga
     4921 atatactttt ccttggtgct ttgtgaatga tagtcaaatt caaaagacca gtcgtgattt
     4981 cctagaacat ataatgaggg aactttgctc ctgctaacgt tatctgcaat tgctttaata
     5041 ttcgcctctg ttgggaaatc ggcaatgtca cctgtaagga atatcatgtc agcgtttact
     5101 ttttctgcgt attcaaagaa agctgggaat ctatcaccag aggggatccc gtctgcggtc
     5161 ttgaatactt gtgacctgcc ataagctgat gcttttactt cttttgtgtc atcgtcagaa
     5221 aagagtgtaa gatgactgtc agttacatgt acaaaaatgt aatccttctt tataccatca
     5281 attttcaatt ctgtctcaat aggagaaatt atcatgtcaa gaccatctcc tttttcataa
     5341 gtctttttgt taacaacaat cttgccttta ttgttaaact tttccaaaaa agtttttata
     5401 ccggtatatg taccatattc tgtactcccc tttatgtata tctttttacc cacaacttcg
     5461 attacat
//
LOCUS       DAONVY010000098         5368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11217_length_5368_cov_3.845097, whole genome shotgun sequence.
ACCESSION   DAONVY010000098 DAONVY010000000
VERSION     DAONVY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11217_length_5368_cov_3.845097"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="PLI11_03815"
     CDS             complement(<1..1675)
                     /locus_tag="PLI11_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPZ52023.1"
                     /translation="MKRRISRIVTFVAICLFAIIYLAGCAKTVNLKHTGFEEGSKDSI
                     KGWILYDYHELHGDPLRTIFDIVPGGVSGNCLKIESKNLNDARVYQEIKVKRNTTYKI
                     TVYVKTENVEEGAGVNISAIDCADVPTESVFGTNSEWQELTVYAKTGPKQRSLQLSLG
                     LGGYGSESSGVAYFDNVSVEVAKKVPQGARVLNVEPRKSSDDKDGKTKHETLMQFLFI
                     TALASLLIYIVALCLKSDKENFTNKEALSYEITRPGKRDWIIIGVMTLVCALFSYYKL
                     GDMKAASNYWKATEAGEYVIVEFDSVKNVRRIAYSCNIPATASYRVLYEDENGEFKQT
                     STLTQKAFFEWEVFKTNFTTKRVKIEARSTGLGINEVGFFETDEDGNLKQIPVKVVEQ
                     KYTEVEGYGKPEYLFDEQDTVPVARTYMNGTYFDEVYFPRTAYEHIHGLKIYETTHPP
                     MGKNFIALGIKIFGMNPFGWRFMGTLAGVLLVPIMYLFALKLFKKRFYAFIAAFLMMF
                     DFMRLAQTRLATIDSYSCLFVLLMYYFMYDYFVVKSYDLTFRRSLRPLIF"
     gene            complement(1659..3761)
                     /locus_tag="PLI11_03820"
     CDS             complement(1659..3761)
                     /locus_tag="PLI11_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52024.1"
                     /translation="MQKKFIAVLLILLVFAFPLFSFAASKVIVSADFNTKEKKGFVED
                     AYFKDEAKYYWSENEGRDGSGCLVVECTTENDARYAYTVKAKKNTYYRISAYIKTENV
                     SYKDENAKGANLSIKDLFDVHGNIFGTQDWTYVEFYGLTGSSQTSFTVFLRLGGYGGT
                     NTGKAYFDDFKVEELDSLPVGAEVTALYKESSANNDFPPMYTDPMRVAGLLTFFNAIV
                     FIIYYRYVTRHKDAVQEGLSTLQKVVLLLFIGLFLRLILSITAPQCSIDVNLFSYWAT
                     NAAKHNFNIYNQVDGIDYPPGYMTILMLVGKIMNLFNAYGTLLGRMLIKMPAILCDIA
                     IAYIIYKLAVKKLSSKWTIFLVCAWLFNPVVLIDSAAWGQVDSVLTLAMVLCIYYITQ
                     EKYATAGVWLAVAVMLKPQALFISPILFYALVKHFTVSKEPIVDKLLHFVKTLCAFLV
                     TFLLIALPYWVNMKSIWLYELFLGTADHYDYITVNALNYHFLKGNNWVRDSTPSVFGW
                     SLFAWGMLAIVAASVITWVVYQINKKEKSVPYLMSAVLIYLVVNFGPRMHERYFFPLI
                     ALMLIALIYINNKWLLYLFAVTSGVNFLIVMEIMTDLTVGGSGGKYRHFNWPHVTTFR
                     GTLAFINVTCSVLLLVFAVMHTMKLFKGDSHLLWKDKTEEMIVPVSGGFIDRIKNKMA
                     RFKRSDIQEWSVDNEKKN"
     gene            complement(3778..>5368)
                     /gene="pepF"
                     /locus_tag="PLI11_03825"
     CDS             complement(3778..>5368)
                     /gene="pepF"
                     /locus_tag="PLI11_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPZ52025.1"
                     /translation="LEKMIVYAIMRRDEDTRESFYQKMYDRCGSLFTDINAALAFFEP
                     ELLAMDKEKIDAFFENTEGLSLYRFKIDEIMRKKPHILSEAEERIMALSGDAIDTADN
                     VFSMFNNADLKFPEVKDEDGNMKELTQARYISFLMSENREVRRGAFKALYETYGKWRN
                     TLASCLSGQVKSNRYVARARNYDSCIEMYLDNDFVKTDVYNNLIETVESNLDKYQEYM
                     ELKRKAMGLDEIHMYDIYAPIVKVPQKKYTFEEGKKIVLEALKPLGEDYLKLVKKSFD
                     EGWIDVYENVGKRSGAYSISCYGVHPYVLLNWQGTLNDVFTLAHELGHAMHSYLSNKN
                     QPFVYARYKIFVAEVASTVNENLLIDYLMNNTDDENIKAYLVNHYLEEFRGTVYRQVM
                     FAHFERDMHALYESGQALTQETLCKLYYDLNLKYFSPAVVVDKDIEMEWARIPHFYSS
                     FYVYKYSTGFSAAVAFAKNILSGDKTLADKYLNLLKSGGNNYPIEQLKLAGVDLSKPD
                     PIEDALNVFGENVKKLSALIV"
BASE COUNT         1584 a         1255 c          934 g         1595 t
ORIGIN      
        1 cgaagattaa cggccttaac gaccttctaa aggtcaaatc gtaggatttt acgacaaagt
       61 agtcgtacat aaagtagtac attagtagta caaacaaaca cgagtacgag tctattgtcg
      121 caagtcttgt ctgagccagc ctcatgaagt caaacatcat aaggaaagct gcaataaatg
      181 cgtagaatct ctttttaaac agtttaagtg caaacagata cataattgga actaaaagca
      241 cgcctgcaag cgttcccatg aatctccatc caaaaggatt catgccaaaa atctttatcc
      301 ctagcgcaat aaagttcttg cccatgggtg gatgtgtggt ttcgtaaatc ttcaatccat
      361 gaatatgctc ataagcggtt cttgggaaat aaacttcgtc gaaatatgtt ccattcatgt
      421 atgttcttgc aacaggtaca gtatcctgtt cgtcaaacag atactcaggc ttgccatagc
      481 cttcaacttc cgtatacttt tgctcaacca ccttgactgg tatctgttta agattgccat
      541 cttcatcagt ttcaaagaag ccaacctcat ttattccaag ccctgtgctt cttgcttcta
      601 tcttgactct ttttgttgta aagttggttt tgaaaacctc ccactcaaag aaagcctttt
      661 gagtcaatgt cgatgtctgt ttgaactctc cattctcatc ctcatacaaa accctgtaag
      721 aagctgtggc aggtatgttg caggaatatg caattctcct gacatttttg acagaatcaa
      781 attctacaat aacatactct ccggcctctg ttgccttcca gtaatttgac gcagctttca
      841 tatcgcccag tttgtagtat gaaaacaaag cacaaaccaa ggtcataact ccaataataa
      901 tccagtctct cttgcccggt ctggttattt catacgacaa agcttcttta tttgtaaaat
      961 tctccttatc gctcttgaga cacaatgcta caatgtaaat caaaaggctt gccagtgcgg
     1021 ttataaacaa aaattgcatt aatgtttcat gtttggtttt gccatcttta tcatcacttg
     1081 actttctcgg ctccacattc aacactcttg ctccttgagg aaccttttta gcaacctcaa
     1141 cggaaacatt atcaaagtat gctacaccag aagactccga cccataaccg cccaatccaa
     1201 ggctgagctg caaactcctc tgcttgggtc ctgttttggc ataaaccgta agttcctgcc
     1261 attcagagtt tgttccaaaa acactttctg ttggaacatc ggcgcaatca atggcggata
     1321 tgttgacacc tgctccttcc tcaacattct ccgtcttgac atatacggtt atcttgtatg
     1381 tggtatttct ctttaccttg atctcctgat acactctggc atcattgagg tttttactct
     1441 caatcttgag gcagttgcct gaaactccac caggaacaat gtcaaatatg gttctcaaag
     1501 gatcaccatg aagttcatga taatcgtaca aaatccagcc ctttatggag tccttgctgc
     1561 cctcttcaaa accggtgtgc ttaaggttaa cagtttttgc acatcctgca agatatatga
     1621 ttgcaaacaa acaaattgcg acaaaagtaa caatcctact aattcttctt ttcattgtcc
     1681 acactccact cttgaatatc gcttctcttg aatcgtgcca tcttattttt tattctatca
     1741 ataaatccac cactgacagg cacaatcatc tcttccgttt tatctttcca caacaaatga
     1801 ctatcacctt tgaataattt catggtatgc atgactgcaa agacaagcag caataccgaa
     1861 cacgtcacgt taataaacgc caatgtccct ctgaaagttg tcacgtgtgg ccagttgaaa
     1921 tgtctgtatt taccaccaga tcctcctact gtcagatccg tcatgatttc catcacaata
     1981 aggaaattta ctccggaggt aaccgcaaac agataaagca accatttgtt gtttatatat
     2041 atcaacgcaa tcaacatgag cgcaataagc gggaaaaagt atctttcgtg cattctaggc
     2101 ccgaagttga ccacaaggta tataagtact gcggacataa gatatggaac agacttttcc
     2161 ttcttgttaa tctggtacac cacccaggtt atcacggaag cagcaacaat tgccaacatg
     2221 ccccaggcaa acagtgacca tccaaaaaca gacggagtgc tatctctaac ccaattgttt
     2281 cctttaagga agtggtagtt aagcgcgttt accgtaatgt agtcataatg atctgctgtt
     2341 ccgagaaaca gttcatatag ccatattgac ttcatgttta cccagtaagg aagagcaatc
     2401 aaaagaaaag tcaccaaaaa cgcacacaac gttttgacaa aatgcaacaa cttgtctaca
     2461 atcggttctt ttgaaacagt aaaatgtttt accagtgcgt aaaacagtat tggggaaatg
     2521 aacagtgcct gaggcttcaa cataacagcc accgcaagcc atactcctgc cgtcgcatac
     2581 ttttcctgtg taatgtaata aatgcacaac accatcgcca atgtcagcac tgagtcaacc
     2641 tgcccccagg ctgccgagtc aataaggaca acaggattga acaaccacgc acaaaccaag
     2701 aaaatggtcc atttgctgct cagtttctta acagccagct tgtatataat gtacgctata
     2761 gcaatatcac ataaaatggc aggcattttt ataagcattc tacccagaag cgttccataa
     2821 gcattaaaaa ggttcattat ctttccaacc agcataagga ttgtcatata cccgggagga
     2881 taatctattc catccacctg gttatatatg ttaaaattgt gtttcgcagc attcgttgcc
     2941 caataggaaa aaagatttac atctatggag cactgtggtg cagttataga gaggataagt
     3001 ctcaagaaaa gaccaatgaa gagcagcagc accacttttt gcagtgtgga gagcccttcc
     3061 tgcaccgcat ctttatgccg ggttacgtat ctgtagtaaa taatgaaaac tatagcgttg
     3121 aaaaaggtga gcaaaccagc caccctcatg gggtccgtgt acatgggcgg aaaatcgtta
     3181 tttgcagaag actccttgta aagagctgtt acctccgcac ccacaggcag ggaatccagc
     3241 tcctctacct tgaagtcatc aaaataggcc tttccggtat tggttccccc atagccgccc
     3301 aggcgcagaa aaacagtaaa tgaagtctgg gaggaaccag ttagaccgta aaactccaca
     3361 tatgtccaat cctgggttcc aaatatgttg ccatgtacat caaacagatc ctttatagaa
     3421 aggtttgcgc cttttgcgtt ctcatcctta taggaaacgt tttcggtttt aatgtacgca
     3481 gaaatcctgt agtaggtgtt tttcttggct tttacagtat atgcatatct tgcatcattc
     3541 tccgttgtac actctacaac aagacacccg ctaccatccc ttccctcgtt ttcactccaa
     3601 tagtatttgg cttcatcttt gaaataagca tcttcaacaa agcctttttt ctcctttgtg
     3661 ttgaaatccg ccgaaacaat gaccttgctg gcggcaaatg aaaacaaagg aaaggcgaat
     3721 actaacagta tcaacaatac ggcaataaac tttttttgca tatttcctcc tatgaaacta
     3781 tacaattaac gcactcagtt ttttcacatt ttcaccaaat acattaagtg cgtcttctat
     3841 aggatcaggt tttgacaaat caacacctgc aagtttaagt tgctctatag gataattatt
     3901 gcctccgctc ttgagaagat tcaaatattt gtcagcaaga gttttatctc cactcaggat
     3961 gttcttggca aaagcgaccg ctgctgaaaa tcctgtggaa tacttgtata catagaagga
     4021 tgaatagaag tgcggaatac gagcccactc catctcaata tccttgtcca caaccaccgc
     4081 aggactaaaa tatttgaggt tgaggtcata gtaaagcttg cacagagttt cctgtgtcaa
     4141 agcttgtcct gattcgtaaa gagcgtgcat gtccctttca aagtgtgcaa acatgacctg
     4201 tctgtataca gtacccctga actcctccag atagtgattt acgagataag ccttgatgtt
     4261 ttcgtcatca gtattgttca taaggtaatc tataaggaga ttctcattga ctgttgaggc
     4321 aacttctgca acaaagattt tgtatcttgc gtatacgaaa ggctgattct tgttgctcaa
     4381 atatgaatgc attgcatgtc ccaattcgtg agcaagtgta aacacatcat ttaaagtgcc
     4441 ctgccagttt agaagcacat atggatgcac tccataacaa cttattgagt aagcaccgga
     4501 tctcttcccc acattctcat aaacatctat ccagccttcg tcaaagcttt tctttaccag
     4561 tttaagataa tcctctccca agggtttcaa tgcttcaagg acgattttct ttccttcttc
     4621 aaaggtgtat ttcttttgag gaaccttcac aataggagca tatatatcat acatgtgaat
     4681 ctcatcaagc cccatggctt tcctttttaa ctccatatac tcctgatact tgtccaaatt
     4741 gctttcaaca gtctcaatca ggttgttgta tacatccgtc tttacaaaat cattatccaa
     4801 gtacatttca atacaggagt cgtaattcct tgctcttgca acgtatctgt tggatttgac
     4861 ctgccctgaa agacatgatg ccagagtgtt tctccatttg ccataagtct catacaatgc
     4921 cttaaatgca ccccttctga cttccctgtt ttcagacatc aggaaggaaa tgtatctggc
     4981 ctgtgtaagc tccttcatat tgccgtcttc atccttgact tcagggaact tcaagtcggc
     5041 attgttaaac atggaaaaca cattgtcggc agtatctatt gcatcgccgg aaagagccat
     5101 tattctctct tcagcttcag acaaaatgtg aggttttttc ctcattattt catctatctt
     5161 aaacctgtac agagacaatc cctcagtgtt ttcaaaaaag gcgtcaattt tttctttgtc
     5221 cattgccaaa agctcgggct caaagaaagc caacgcagca tttatgtctg taaacaaaga
     5281 gccacatcta tcatacatct tctgataaaa gctttctctt gtatcttcat cgcgacgcat
     5341 gattgcataa acaatcattt tctccaac
//
LOCUS       DAONVY010000099         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11492_length_5256_cov_3.793694, whole genome shotgun sequence.
ACCESSION   DAONVY010000099 DAONVY010000000
VERSION     DAONVY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11492_length_5256_cov_3.793694"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLI11_03830"
     CDS             <1..338
                     /locus_tag="PLI11_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52026.1"
                     /translation="GQDQDGGEGKGGEGNGSGAGKGEGKETKEDVSIYDPSLIGGDGK
                     DEYVPGKKDENGNVQKIELGEAELKEGTVPYDEVFQDYLNKARQAIERESVPDDIRSL
                     IEKYFSSLE"
     gene            350..1336
                     /locus_tag="PLI11_03835"
     CDS             350..1336
                     /locus_tag="PLI11_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPZ52027.1"
                     /translation="MITEKDIYGFHDLVGKISKEIAKDMVGQTDVIEGVLIAMISGGN
                     ILLEGVPGLGKTRLVRTIGRVFDLPFSRIQFTPDLMPADVVGTNIVVKDEMGNSVFKF
                     QPGPIFSNIVLADEINRATPKTQSALLEAMQEHTVTVGGVSRKLDEPFFVLATQNPIE
                     QEGTYPLPEAQMDRFMFKLLVKFPSPDELTNIVRMTQKKNDETADKVATGEDILKLRE
                     VARGVPVADPVMDYAMRLITRTHPDLDEGAPSSKKYLRFGASPRGAQALISGAKVRAL
                     MNGRYNVSFEDIEVLSYSVLRHRIALNFEAMTEGLTSDMVIERIISETGRGV"
     gene            1338..2219
                     /locus_tag="PLI11_03840"
     CDS             1338..2219
                     /locus_tag="PLI11_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPZ52028.1"
                     /translation="MSGTLFDEGFMSKLENMSLHFKNAVNSVYTGGRRSKSHGSTVEF
                     SDFREYTPGDDYRRIDWNAYARFEKFFIKLYLDEKQLHTRILLDTSKSMDFGEPNKGF
                     LAKRLAAAFAYMAIGSLDRASIYSVSEHMEHVCSGIEGRNAFYKSLERLAAVPFKGES
                     RLFECVKDIRNIYRGDGAAIIISDLLTDSDYKAMIDYLLYYKQQIILVHILSPDELNP
                     QYSGRVRLLDSEGNDYRDLDITPRTLDLYKRTLRSFMDDIASFCAKRGVYLLQISSDE
                     SLESILLGKGHSLGIIS"
     gene            2235..4058
                     /locus_tag="PLI11_03845"
     CDS             2235..4058
                     /locus_tag="PLI11_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HPZ52029.1"
                     /translation="MSFINPWGLISFISIPILIVLYILKQQHSEHVVSSTYLWKKTEM
                     FMQASTPLQKLRKNLLFFLQLLMLLFLSLAISRPVILSEALDEDFIVIIDTSASMRAE
                     DVSPNRFEYSKKEILRLVNGLLPGQKMSLIEAGNTVTLLVSRSDNKQVLREALEKLEC
                     GYSDANIDGAYSLADSIIKESNTSKVILYSDKDYEDSGNLSIVNVAKSRENVAITQAT
                     AAYVDDSYTVMSTVVNYGSDKSVTLELYVDDRLVDAKTVLCEKEKPVHTYWRNISQEA
                     GFVKIKITEKDVLPIDNEAYAVLSKNERQKILLVSEESYFIEKVLQAIGNFDIYKTTK
                     DKAESQKGFDLYIYDGFVPSSFQNDGNVWVINPTRSFLGLSLGDLIKGSALDSVETFS
                     GKALSTYIDAANIYLARFWAVTSHNDWEVAMKCGELPAIVTRTTESSQQFIALLFNIS
                     ETNLPLLKEFPVLVQNMLTYTMPSLLDGDVKFVSGEHIEIRALPNAQKVEVTSPKNVK
                     TTVAPPFPPRGYEATVPGLYHIRQEVRRGEDNIKVNEGYFTVSIPGNESALLSKGHSL
                     DIGESTNQKVKGAIELWPYLLLLVIFIGMTEWWVYYRGNQL"
     gene            4042..>5256
                     /locus_tag="PLI11_03850"
     CDS             4042..>5256
                     /locus_tag="PLI11_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPZ52030.1"
                     /translation="MGISFERPMFLILAIPLLAFTLIPWFKLSKTTRRSGKHVLSLTL
                     RIICIVLTTLLLSGFMTVKQTKKTSVIILADLSDSTRHVRQQMNNYVRDAVEHADKET
                     RIGLMTFGYDTVYELPLTTDLRFVSFETSPQGNHTNLANAIIMANAMMPSDTNRRIII
                     LTDGKQTIGDALESARQVAAQGVRIDAILLETGTVGKEVQVNKINLPETLYVGEEFEL
                     VVEIESNYKADAVLRLFDEQELKTVQNISLQEGINKFVFTDVATAPGVKNYRVEIETK
                     EDDILRNNRMYSYIRVLGTPRLLIIDGTGNESHEFVKILGDTANYDVVSPRMAPTTLE
                     NLRKYESVVMMNVSKSDLPQGFDDMLEQYVRQLGRGLLTVGGDESYALGGWADSTLEK
                     ILPVNVYQRDEGE"
BASE COUNT         1640 a          925 c         1295 g         1396 t
ORIGIN      
        1 aagggcagga tcaagatgga ggtgaaggca aaggaggaga aggaaatggc tctggcgccg
       61 gaaagggaga aggaaaggaa accaaggaag atgtaagcat ctatgatcct tcactgattg
      121 gaggagacgg caaagacgaa tacgtgcctg gcaaaaagga cgagaacgga aacgtacaga
      181 aaattgagct gggagaagct gagttgaaag agggcaccgt accgtatgat gaagtcttcc
      241 aggactattt gaacaaggca aggcaggcaa ttgaaagaga aagtgttccc gatgatatca
      301 ggagtttaat agaaaaatat ttttcatcac ttgagtaatg gaggacttta tgataactga
      361 aaaagacata tacggttttc atgatttggt gggcaaaatc tctaaagaga tagccaaaga
      421 catggttggt cagacggatg ttattgaagg cgtacttatt gctatgattt ccggtggaaa
      481 tattcttctt gaaggtgttc ccggacttgg aaaaacgaga cttgtgagga caataggcag
      541 agtttttgat ttgccttttt caagaataca gtttaccccg gatttgatgc ctgcggacgt
      601 ggttggtacg aatattgttg taaaggatga aatgggaaat tcggtattta aattccaacc
      661 aggaccgata ttcagcaaca ttgtgcttgc agacgagata aatagggcca ctccgaaaac
      721 ccaatccgca cttcttgagg caatgcagga gcatactgta actgttggtg gagtgtcaag
      781 aaaacttgat gaacctttct ttgttttggc cacccagaac cctattgagc aggaaggcac
      841 ttaccctttg cctgaagcac agatggacag gtttatgttt aaactgcttg tgaagttccc
      901 atctcctgat gaacttacta atatagttag aatgacacag aagaaaaatg acgaaactgc
      961 agacaaggtg gcaacaggcg aggatatttt gaaacttagg gaagtggcaa gaggtgtccc
     1021 tgttgcagac cctgtaatgg actatgccat gagacttata actaggacgc atcctgatct
     1081 ggatgaaggg gctccaagca gcaagaagta tttgagattc ggagcaagtc caagaggtgc
     1141 gcaagccttg atttcaggag caaaagtaag ggctcttatg aatggaaggt acaatgtttc
     1201 atttgaagat atagaggtgc tcagctattc tgtcttaaga cacagaatag cgctgaattt
     1261 tgaagccatg actgaaggtt tgaccagcga catggtaata gagagaatta tcagcgagac
     1321 cggcaggggc gtttgatatg agtggaacac tttttgatga aggcttcatg tcgaagcttg
     1381 aaaacatgtc cctgcacttt aaaaatgctg ttaacagcgt ttacacaggc ggaaggcgtt
     1441 ccaaatccca tggtagcact gtagagttct cggacttccg ggaatatact ccaggagatg
     1501 attacagaag gatagattgg aatgcttatg caagatttga aaaatttttt ataaagctct
     1561 atctggatga aaaacagcta catacaagga tactgcttga tacttcaaag tcaatggatt
     1621 ttggcgagcc aaacaaaggt tttcttgcca agaggcttgc cgcagctttt gcatacatgg
     1681 caataggctc attggacaga gcttccatat attccgtttc tgagcatatg gagcatgtat
     1741 gttctggcat tgaagggcga aatgcctttt acaagtctct tgaaaggctt gcggcagttc
     1801 ctttcaaagg tgaatccagg ctgtttgaat gtgtcaagga tattaggaac atatatcgcg
     1861 gggatggagc tgcgataata atatctgatc ttctaacaga cagcgattac aaggcgatga
     1921 tagactatct tctgtattac aaacagcaga taatacttgt ccacatattg tcaccggacg
     1981 aattaaaccc ccagtacagt ggaagggtaa gacttttgga cagcgagggt aatgattaca
     2041 gggaccttga tattactcca aggactttgg atttgtacaa aaggacttta aggagcttta
     2101 tggatgatat tgcatctttt tgtgcaaagc gtggagtgta tctgttgcag atatccagtg
     2161 atgaaagcct tgagagcata ttgctgggta aaggtcatag tctgggaata ataagctgat
     2221 taatggagag tgctatgagt tttataaacc cctggggttt gatttcattc atatcaatac
     2281 caatattaat agtcctgtat attttaaaac agcagcacag cgagcatgtg gtgtccagca
     2341 catatttgtg gaaaaaaacg gaaatgttta tgcaggcatc cactcccttg cagaagctga
     2401 gaaagaacct tctgtttttt cttcagctat taatgctttt atttttgtct ctcgccatat
     2461 cgcgacctgt aatacttagt gaagcccttg acgaggattt tatagtaatc atagatactt
     2521 ccgccagcat gcgagcagaa gatgtatccc caaacaggtt tgaatattcc aaaaaagaga
     2581 tattaaggct ggtgaatggt cttttgccag gccagaaaat gagccttatt gaagcaggca
     2641 acactgtaac gcttcttgta tccaggtccg acaataaaca ggtgctgaga gaagccttgg
     2701 aaaaactgga atgtgggtat tcggatgcaa atattgacgg tgcttattct cttgccgaca
     2761 gtattataaa ggagagtaac accagcaagg taatattgta ctcagacaag gattatgagg
     2821 acagtggcaa tttgagcatt gtaaatgtgg caaaatcaag agaaaatgtg gcaatcaccc
     2881 aagccactgc cgcatatgtt gacgacagct atacggtgat gtcgacagta gttaactacg
     2941 gaagtgacaa aagcgttaca ttggagctat atgtcgatga caggctggtg gatgcaaaaa
     3001 ccgtactatg cgaaaaggaa aaacccgttc acacatactg gagaaacata tcgcaggaag
     3061 caggctttgt gaaaataaag attacggaga aggatgtatt gcccatagac aatgaagctt
     3121 atgcggtatt gtccaagaat gaaagacaaa agattttgct agtaagtgag gaaagctatt
     3181 ttatagaaaa agtgctgcag gcaatcggaa attttgacat atacaaaaca acaaaggaca
     3241 aggcggaaag tcaaaagggc tttgatttat atatctatga cggatttgta ccgagcagct
     3301 ttcagaatga tggaaacgta tgggttataa accctaccag gagctttctg ggattgtccc
     3361 ttggtgattt aattaaggga agcgcattgg attctgttga aacattttca gggaaggcgc
     3421 tttccacata tattgatgct gccaatattt atcttgcaag attctgggca gttacatccc
     3481 ataacgattg ggaagttgcc atgaaatgcg gcgaattgcc tgccattgtg actcgtacaa
     3541 ctgaatcatc ccagcagttt atagcacttt tattcaatat atcggaaacg aatctgcctc
     3601 ttttgaaaga gtttcccgtt cttgttcaga acatgctgac ttacactatg ccctctttgt
     3661 tggatggcga tgtcaagttt gtatctggag agcatataga gatacgggcg ctgccaaatg
     3721 cgcagaaggt ggaagtaaca tctcccaaga atgtaaaaac caccgttgcg cctccatttc
     3781 caccaagagg atatgaggca acagtgcctg gactgtatca tataagacag gaagtacgca
     3841 gaggcgagga taacataaag gttaatgaag gttactttac agtatcaatt ccaggcaacg
     3901 aatcagcatt gctttctaaa ggccattccc tggatattgg agaaagtacc aatcaaaaag
     3961 tcaagggagc gattgaactt tggccttatt tactactcct tgtgattttt atcggaatga
     4021 cggaatggtg ggtgtattat cgtgggaatc agctttgaaa gacctatgtt tcttatattg
     4081 gcaattcctt tgcttgcctt tacgctaata ccctggttca agctttccaa aacaaccaga
     4141 agaagcggaa agcatgtttt gtccttgaca ttaaggataa tctgcattgt tttgactaca
     4201 ttgcttctgt caggttttat gactgtaaag cagacaaaga agacctcggt tataattctt
     4261 gctgacttgt ccgacagtac aagacatgta aggcagcaga tgaacaacta tgtcagggat
     4321 gctgtggagc atgcagacaa ggagaccaga ataggtctta tgacttttgg gtatgacacg
     4381 gtatatgagc tgccattgac cacggatttg agatttgttt cttttgagac atccccgcag
     4441 ggaaaccata caaatctggc caacgcaata attatggcca atgccatgat gccttctgat
     4501 accaatagaa gaataattat actgactgat ggaaagcaaa ccatagggga tgccctggaa
     4561 agtgcaagac aggtggctgc acagggtgta aggatagatg ctattctatt ggaaaccgga
     4621 actgttggca aggaagtaca ggttaataaa atcaacctgc ctgaaaccct ttatgtaggc
     4681 gaagagtttg aacttgttgt tgagattgaa agtaattata aagcggatgc tgtattaaga
     4741 ctgtttgatg agcaggaact gaagactgtt cagaacataa gcctacagga aggcatcaac
     4801 aaatttgtgt ttacagatgt ggcaaccgca cccggtgtga agaactacag ggttgaaatt
     4861 gaaacaaaag aggacgatat attaagaaac aacagaatgt acagttatat tagggtattg
     4921 ggaacgcccc gtcttctaat catagatggt acgggcaacg aatcccatga gtttgtaaaa
     4981 attcttgggg atactgccaa ttatgatgtc gtatcgccta gaatggcgcc taccactttg
     5041 gagaatctgc gcaagtatga aagtgttgta atgatgaatg ttagcaagtc cgatcttcca
     5101 cagggatttg acgatatgct ggagcagtat gtacgtcagc tgggaagagg tctccttaca
     5161 gttggaggag atgaatccta cgcacttgga ggttgggcgg acagtacttt ggaaaagatt
     5221 ctacctgtta atgtatatca gagggatgaa ggggaa
//
LOCUS       DAONVY010000100         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11493_length_5256_cov_3.591809, whole genome shotgun sequence.
ACCESSION   DAONVY010000100 DAONVY010000000
VERSION     DAONVY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11493_length_5256_cov_3.591809"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLI11_03855"
     CDS             complement(<1..582)
                     /locus_tag="PLI11_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006210877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ52031.1"
                     /translation="MSNIVIRKMSDYDRDVSYDVANVFVDGYYKDLSFFSKDREKLCS
                     AFKDTFCPDVFYLAELDGKIAGILACSNNKHRAMHIDKSSMSHGLGFLMGGIAFMLLN
                     KEFNTPLDFTDETAYIECVATSEDARGKGVCTALFKHVMQELSYREYVLDVADTNMNA
                     IRLYKKLGFEEFMRKLEKNAKLKGFTARIYMKWC"
     gene            complement(598..1194)
                     /locus_tag="PLI11_03860"
     CDS             complement(598..1194)
                     /locus_tag="PLI11_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPZ52032.1"
                     /translation="MARTKEQNERMTMATRKKIMEAGLVLFAQKGFALTSIKDIAEAA
                     GISTGLIYRHFSSKEDLFNSLIANTIEELNKFIQILDSYDSPKQAIVEMTASLIGDIH
                     FSNELSRYFLIVIRSMLEEEVLSQIDEFRKSILSLYDKTKDLIKEGQKRGEFKQGNPY
                     KYTLLFFSIIQGMANMKLFMGDKYVAPELDDVLGFLLI"
     gene            complement(1259..1689)
                     /locus_tag="PLI11_03865"
                     /pseudo
     CDS             complement(1259..1689)
                     /locus_tag="PLI11_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
     gene            complement(1734..2087)
                     /locus_tag="PLI11_03870"
     CDS             complement(1734..2087)
                     /locus_tag="PLI11_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HPZ52033.1"
                     /translation="MVFVCYPRCTTCQKARKWLVENNLEFTERNIKEENPTLEELKEW
                     HKKSGLPLKKFFNTSGMKYRELNLKDRLTEMSDEEQFELLSSDGMIVKRPILVGDDFV
                     LVGFKESEWKASLGI"
     gene            complement(2089..2616)
                     /locus_tag="PLI11_03875"
     CDS             complement(2089..2616)
                     /locus_tag="PLI11_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ52034.1"
                     /translation="MRVDLKQVEEDEKEVLRNLLEKYDYEFSQYDKRDVNDLGLYGYK
                     YLDYYWTEENRWAYFILVDDKLAGFVMVNDYSEVDFRETDFQLAEFFVMYKYRRAGVG
                     RQAFFKVLDLHKGRWQLRRHPANTSSVYFWDKVIDEYTNGNYELIRACPGAMYDDGTL
                     GDVFFFENRRNIEED"
     misc_binding    2721..2940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3008..3115
                     /locus_tag="PLI11_03880"
                     /pseudo
     CDS             3008..3115
                     /locus_tag="PLI11_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family accessory protein
                     TnpB-related protein"
     gene            3324..4532
                     /locus_tag="PLI11_03885"
     CDS             3324..4532
                     /locus_tag="PLI11_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52035.1"
                     /translation="MTDYTVDGLSAANPPFTKVDIRAYATTELLGATGIRTTYKLPGP
                     PATPDSLTIIQDVNVIGLSFADSYIVVRVEIINHNNVDTKIGNRYLWDPIIEGDDGPT
                     FQTVNPIGPMITNETEFEHPLFEAFQLVDNDNPNPPTYITYGSVAGPQSLRLGTRPTM
                     IKYVSWATSGGTTFDYTIDPNRVVSSPPINDSAVLYYWGHNMETAIVLEACGGKAARA
                     AALFATKPGIVPPFINSTICVNVNRILDTCRQEVIHTETHEIPSLNCGVLLDCKVKKA
                     KCVVKKETEDTVQVQVKLRVAFKIKIGGKKMTVVKPFIINSTAHLMVPEKATVSCEVQ
                     NPRCVSRQIDECGLVETTVCAFIKIQSEVKESIVIPFVASCPVGLCSRVVTENEDHNS
                     DLIDESDETM"
     gene            complement(4638..>5256)
                     /locus_tag="PLI11_03890"
     CDS             complement(4638..>5256)
                     /locus_tag="PLI11_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52036.1"
                     /translation="PTYPKGWIYKKSTGVSKTISGFKKLVRSDVLFFEYFGYPWIFEN
                     KAMLAELLNGNSINFKGSIFESMITSSYMVGWNYVESDKDAEEFMKQVFNFHDSVLRT
                     ANYISGAYVNPDKTMYPEAKIRQATLVFDSQWCDSIEMVFEGVTAFNLRPPEDNYSAD
                     IYGATLIVKDESVFFCDDIVAGWDENQIGTWITAYGLRWRFVSKD"
BASE COUNT         1598 a         1188 c          853 g         1617 t
ORIGIN      
        1 acaccatttc atgtagattc tcgcagtaaa acccttaagt ttggcattct tctccaactt
       61 tctcataaac tcctcaaatc ccagtttttt atacaacctg attgcattca tattggtatc
      121 cgccacatcc aaaacatact cacggtacga cagctcctgc atgacatgtt tgaacaacgc
      181 tgtacataca ccttttcctc tggcatcctc acttgttgca acacattcta tatatgcagt
      241 ttcatcagta aaatccaaag gggtgttaaa ttccttgttc agcagcatga atgcaatacc
      301 acccattaaa aaacccagtc catgactcat tgaactcttg tcaatatgca tggcacggtg
      361 tttgttattg gagcatgcca gtattcccgc aatctttcca tccaactctg ccaaataaaa
      421 aacatcagga caaaaagtat ctttaaatgc actgcaaagc ttttccctgt ccttgctgaa
      481 aaaggacaag tccttgtaat atccgtctac aaacacattt gccacatcat agctaacatc
      541 tctgtcataa tcactcattt tacgtataac tatattactc atttttaacc tccatatcta
      601 aatcaataaa aatcccaata catcatccaa ctctggagct acatatttgt cacccatgaa
      661 tagtttcatg tttgccatgc cttgtattat tgagaaaaac agcaaagtgt atttgtacgg
      721 attgccttgt ttaaactctc ctcgtttttg tccctctttg ataaggtctt ttgttttatc
      781 gtataaagac aatatacttt tcctgaactc atcaatttga gaaagcacct cttcctccag
      841 cattgagcga ataactatta gaaagtagcg cgacaattca ttactaaaat ggatgtctcc
      901 aattaaactt gctgtcattt ccacaatagc ctgtttggga gaatcatacg aatcaagaat
      961 ctgaataaat ttgtttaatt cttcaatggt atttgctatg agactgttaa acaggtcttc
     1021 cttggatgaa aaatgccgat atattagccc ggtgctaata cctgcagctt ctgcaatgtc
     1081 ttttatactc gtcaatgcaa aacccttttg cgcaaacaaa acaagtcctg cttccattat
     1141 cttttttctt gtcgccattg tcattctttc attctgctcc tttgttcttg ccataacaaa
     1201 acctccttaa tacaataaac tgaatttaaa ttcattataa tagaataaaa aaagcacgtc
     1261 aactttgtcc gtacttttct tcaagataac tcactgtggt gcaaataagc ctttcaagcg
     1321 cctccatgtc tatatccgac agtttcttaa catatataca tgccttgccc atcgtgaact
     1381 ttcccaagtc tttcaacaag tactcatgat ccggatcccc agtatatacg tacaaggaaa
     1441 tagctgcttt gcgaggagaa aatgccacga gcggccaatc cccttcctgc ctgctgcgct
     1501 cggatttgta atgatagctg ccaacaccaa tcattgaatc cccccacata tctttggggg
     1561 atatcctgtt accttttgca taaactctac aagcttataa ctgtcctgct ttttctgttc
     1621 actattcgca tatgaatcga taaactcaaa aacatctcca tcatgctgct ttgtctttaa
     1681 ttctgacatt ttctcacttc ctcatatttt ttccaaagtt caatagcaga aagtcatata
     1741 ccaagagatg ctttccattc actttcctta aacccaacca gtacaaaatc atcaccaaca
     1801 agaatgggac gtttaacaat cattccgtct gacgacaaaa gttcaaactg ttcttcatca
     1861 ctcatttccg taagtctgtc tttcagattc aactccctgt atttcatgcc gctggtgtta
     1921 aagaactttt ttagaggtag tccgctcttc ttatgccatt ccttcaattc ttccagagtc
     1981 ggattctcct ccttaatatt tctctctgtg aactccagat tgttctcaac aagccacttc
     2041 cttgcctttt gacatgtagt gcatcttgga tagcatacaa aaaccatatt aatcctcctc
     2101 aatatttctt ctattttcaa aaaagaacac atcacccaat gtaccatcgt catacatggc
     2161 accaggacat gctcttataa gttcataatt cccatttgta tattcatcta ttaccttgtc
     2221 ccaaaaatat accgatgaag tattggcagg gtgtcttctt aattgccatc tgcctttatg
     2281 taagtcaagc accttgaaaa acgcctgcct gccaactcct gcccttctat acttgtacat
     2341 aacaaaaaac tccgccagtt ggaagtctgt ctccctgaaa tccacctctg aatagtcatt
     2401 caccatgaca aaccctgcta gtttgtcatc tacaagaata aaatatgccc accggttttc
     2461 ctcggtccag taatagtcaa gatatttata gccatataga cctaaatcgt tgacatcacg
     2521 tttgtcatac tgagaaaact cataatcgta tttctccaac agatttctca gtacctcctt
     2581 ttcatcttct tcaacttgct ttaagtccac cctcacctaa ttttacctgc tttcataaaa
     2641 tctcttaatc aatactatac atcataatat tctccttgac aattttatct ttaagagcat
     2701 attatatgta aaataacata agaaaagcaa tgacggaaac gagtaaacta caggaagcat
     2761 acagacagag gaatatttgg tggaagttcc ttatgtgaaa tgtagttgaa aacccttcct
     2821 gagctccaat gcggaaatta tagtatgtgt tgccgtgcac ctgcgttaaa ggttaggatt
     2881 tgttggaatc cgaattgaaa agttaaggtg gaaccgtgca ggaatacttg cacccttgga
     2941 cagaacaatt tctgtttaag ggtgcctttt gttaatttaa ttccatgttt tcttatgtta
     3001 ttacaaaaaa cagctgcata aatggtcttt ctacagactt gctagtttta tccaatataa
     3061 gggaagttta gcgggtatta aggttgaata tgttaatcca caaaatacaa gtcaaaacca
     3121 ggttcattct taccctctgg aggtataatt tttacagatt ctatgttatg tggaattgtt
     3181 attacactat tattcaataa agcatgtttt aaatctatac agccaccaca gggccaagcc
     3241 atccactagg acctggaagg aatcttttat tcggaggtgg tgcgcccggt acaacattca
     3301 atacatttcg ttcatataca actatgacgg attacacagt tgacggcttg tcagctgcca
     3361 atccgccatt tacaaaggtg gatataaggg catatgccac tacagaactt ttgggtgcaa
     3421 caggcataag aactacatat aaattgcccg gaccaccagc cacgcctgac tcattaacaa
     3481 ttatacagga tgtaaatgta ataggtttat cctttgcaga ctcatatata gttgtcaggg
     3541 ttgaaataat caaccataac aatgtagata ccaaaatagg taatcgctac ctttgggatc
     3601 ccataataga aggtgatgac ggacctactt tccaaacagt taaccctatt ggtccgatga
     3661 taacaaacga aacggaattc gaacatccgt tatttgaagc tttccaacta gttgacaatg
     3721 acaacccgaa tccaccaaca tatatcacat atggttcagt agcgggtcca caaagtcttc
     3781 gtttaggcac tcggcctaca atgataaagt atgtcagctg ggctacaagc ggaggcacca
     3841 cctttgacta taccatcgac ccaaacaggg ttgtttcaag tccacccatc aacgatagtg
     3901 cagtactgta ctactggggg cataacatgg aaacagcaat tgttcttgaa gcttgtggtg
     3961 gcaaagctgc aagagctgct gcattgtttg caacaaaacc aggaatcgta ccacctttca
     4021 taaacagcac catttgtgtt aatgtgaaca ggatactgga cacttgcaga caggaagtaa
     4081 tccacactga aacacatgaa attccatcat tgaactgtgg tgttttactt gactgcaagg
     4141 tcaagaaagc aaagtgtgtt gttaaaaaag aaaccgagga taccgttcaa gtacaggtaa
     4201 aactcagggt tgctttcaag ataaagattg gcggcaagaa aatgactgtt gtaaagccgt
     4261 ttatcattaa ttcgacagct catttgatgg ttcctgaaaa agctacagtg agctgcgagg
     4321 tacagaatcc aagatgcgta agcaggcaaa ttgatgaatg cggtttggtt gaaacaacag
     4381 tctgcgcttt catcaagatt caatcggagg ttaaagaatc tattgtaata cccttcgtag
     4441 caagctgtcc tgtaggactt tgttcgagag ttgttaccga aaacgaggat cataattccg
     4501 atcttataga cgaatctgat gaaaccatgt aatacctgat ctatagccta gaaacaccct
     4561 cgttatattc acatgcatag cgagggttta ttgtgctcaa atttatcact tcttctgccg
     4621 tatacaaata ttataattca gtcctttgaa acaaacctcc atctaagtcc atatgcagta
     4681 atccaggttc caatctggtt ttcatcccac ccagcgacaa tgtcatcaca aaagaatact
     4741 gattcatcct ttacaatgag tgttgcccca tagatatcag ctgaatagtt atcctctgga
     4801 ggtcgcaggt tgaaagcggt tacaccttca aataccatct caattgagtc gcaccattga
     4861 ctatcaaaga ccaatgttgc ctgtctgatt tttgcttcag gatacatggt tttgtcggga
     4921 tttacatatg ctccacttat gtaatttgct gttctgagaa cagaatcatg aaagttaaag
     4981 acttgtttca tgaactcttc agcatcttta tccgattcaa cataattcca accaaccata
     5041 tacgaagaag ttatcatgct ctcaaaaata ctacctttga aatttataga gtttccattc
     5101 agcaactcag ccagcattgc cttgttttca aatatccatg gatacccaaa gtattcaaaa
     5161 aacagaacat cacttctcac aagttttttg aagccggata ttgtcttgga aacacctgtt
     5221 gatttcttgt aaatccaccc tttcggatat gtgggg
//
LOCUS       DAONVY010000101         5248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11521_length_5248_cov_3.562295, whole genome shotgun sequence.
ACCESSION   DAONVY010000101 DAONVY010000000
VERSION     DAONVY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11521_length_5248_cov_3.562295"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLI11_03895"
     CDS             <1..487
                     /locus_tag="PLI11_03895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010404.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPZ52037.1"
                     /translation="YLEADLFNNGVRPAINPGFSVSRVGGSAQVKAMRKLAGPLRIEF
                     AQYKELAAFSQFGSDLDQDTLRRLNHGDRIMEVLKQPQYRPMPVEDQVLIIYALNNGF
                     LEDVDINRILRFQKDLIKYVDTHAVRIKEEIRGKGDISDELAKEIDAVIKEVKELYNY
                     S"
     gene            500..1369
                     /gene="atpG"
                     /locus_tag="PLI11_03900"
     CDS             500..1369
                     /gene="atpG"
                     /locus_tag="PLI11_03900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPZ52038.1"
                     /translation="MNSLRDIKQRIGNIDSTKQIIRAMDMIASTKLQKVRVQLEGVRP
                     IYGNLKRIAEELGCHKDAHSHIFYNNSSIESNDTLYIVITSDRGLSGSYNANILSKSY
                     AHMNQEGVNEKILVVGSKGYEFFKKRNKHILRTVTDLTDAQVYYVAESIANWLTDYYL
                     SGKVKEVYIAYTHFETVLSYVPYVEKLLPLSCSGLKEYFDRKYEPDVKTFIDHLVPIY
                     LHMNLFRAFSESHTSEQAARMVNMSSAGKNAEEIIEDLTRMFNRKRQAVITQELSEIV
                     GSVSILNKGELDG"
     gene            1362..2771
                     /gene="atpD"
                     /locus_tag="PLI11_03905"
     CDS             1362..2771
                     /gene="atpD"
                     /locus_tag="PLI11_03905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPZ52039.1"
                     /translation="MAEVNKGKIVQIIGAVIDIRFESELPSLYNAIEIKNGDEIIVAE
                     VMQHLGDNTVRCISMHPTDGLRRGMDAVDTGNPISVPVGEEVLGRMLNVFGEPIDNQP
                     VVEAKEKWVIHREAPKLSEQVATPEVLETGIKAIDLLCPFAKGGKIGLFGGAGVGKTV
                     LIMELINSIAKEHGGYSVFVGVGERTREGNDLYFDMKNSGVLDKTVLVFGQMNEPPGV
                     RMRVGLTGLTMAEYFRDQKNQDVLLFIDNIFRFVQAGSEVSALLGRTPSAVGYQPTLA
                     TEIGALQERIASTKAGSITSVQAIYVPADDFTDPATATTFAHLDAVTVLSRSIVEMGI
                     YPAVDPLSSSSRILEASIVGSEHYNVSRSVKSLLQRYQDLQDIVAILGIDELSEEDKK
                     IVNRARKVQRFLSQPFYVAEKYTQIPGRYVPIEETVRGFREIIEGVHDDIPEGFFLNV
                     GTIDEVVERYKRSMGDIDG"
     gene            2764..3237
                     /gene="atpC"
                     /locus_tag="PLI11_03910"
     CDS             2764..3237
                     /gene="atpC"
                     /locus_tag="PLI11_03910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPZ52040.1"
                     /translation="MAEAAAKADRKIDLRITTPRGLKFEEKADMIIMRCIDGDMGVLR
                     DHAPISAVLGDGILRIINDGVEQKLALFGGIVEISDNTVDIYSTIAQRPEEIDLERAR
                     RDKEEAEAIIMEETEELKIRSMQVVLRRSLVRIEVSLYLDEENEISIGNTNDDEE"
     gene            complement(3266..3934)
                     /locus_tag="PLI11_03915"
     CDS             complement(3266..3934)
                     /locus_tag="PLI11_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52041.1"
                     /translation="MIELVNINKVYRKGNKNDVHALKDVSLNIEEGELVALVGVSGSG
                     KSTLLHILGCLDDYDSGEYYFKGRNITKLGSKQIARIRNEEIGFVLQSFGLILGNTVY
                     NNIALPLLLSNKTKVSEIELKIDEILKKLGIYQKKKNLVESLSGGQQQRVAIARAIIN
                     NPLLILADEPTGHLDRSTSKDMMDIFHDLNKNEGATILIATHDPFIYEQCHRIIRIDD
                     GRIQ"
     gene            complement(3937..5028)
                     /locus_tag="PLI11_03920"
     CDS             complement(3937..5028)
                     /locus_tag="PLI11_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52042.1"
                     /translation="MKFIKNMMLAFKYGSIFSINRIIIILELIIVLVLGNIAFNHLLI
                     DNANRTELRALVANNIVIYDMEDEEQVEYCTKNGNPIDYNYPYLYFYKESPDRPLRDG
                     FNTRVYSREYLNIVKLPLKIGKWFDDIDIGEYDFIVVIPYKFRKDLKVGKTYKLYTTL
                     EDENAKYTVYVAGLLKPYIHSDEIICLDAKGIIPHEKRYEYNNMKYARLDYFDTNELA
                     QLGIVRFDEEYKRVLEINKSDRGFLVILISTLYTIFLCGFTGDRILNIDTYTKTFATM
                     FLCGATRRKALTMQFLQNIPVVLTSGLISYFLIKYLYRVEFKYMFFNTTAFFGFIAIT
                     LAIHVLLSFLVLSIISKQSPVGIISGRDI"
     gene            complement(5028..>5248)
                     /locus_tag="PLI11_03925"
     CDS             complement(5028..>5248)
                     /locus_tag="PLI11_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52043.1"
                     /translation="IMYTIPIVITAISFGLGTLIYKLFLFGFFKKFDTTLLMLSPNLV
                     YITFFANIVFCFILLLPTALSVMKREVL"
BASE COUNT         1635 a          961 c         1155 g         1497 t
ORIGIN      
        1 atatctggaa gcggatctgt ttaataacgg tgttcgtcct gcaattaatc ccggattctc
       61 ggtatcccgt gttggtggtt cagcccaggt aaaagcaatg agaaagctgg caggaccttt
      121 gcgcatagag tttgcacagt acaaagagct tgccgccttt tcacagtttg gttctgattt
      181 ggaccaggat acattaaggc gtctcaatca tggtgacaga attatggaag tattgaaaca
      241 gccgcaatac aggccaatgc ctgttgaaga ccaggtattg ataatatatg cgcttaacaa
      301 tggcttcttg gaggatgtag atataaatcg catattaagg ttccaaaagg atttaataaa
      361 atatgtggat acacatgctg tacgcatcaa ggaggaaata agggggaagg gcgacatatc
      421 tgatgagctt gccaaggaaa tagatgctgt cataaaagag gtaaaggaac tttacaacta
      481 cagctaggga ggttatggtt tgaattcact aagggacata aagcagagga ttggaaatat
      541 tgattcgacc aaacagataa taagggcgat ggatatgatt gcgtctacaa agctgcagaa
      601 ggtaagagtg cagcttgaag gcgtgcgtcc catttacggc aatcttaaaa gaattgctga
      661 agaactgggc tgccataaag atgcccactc ccacattttt tacaacaact caagtattga
      721 atccaatgac acattatata tagtcataac cagcgataga ggtctttctg gaagctacaa
      781 tgcaaacata ctttcaaagt cgtatgcgca catgaaccag gaaggagtta atgagaaaat
      841 acttgttgta ggttcaaagg gatatgagtt tttcaaaaaa agaaacaaac atattcttag
      901 aactgtaacg gatttgacgg acgcacaagt ctattatgtc gctgaaagta ttgcaaactg
      961 gcttaccgac tattaccttt caggaaaagt caaagaagta tatattgcat acactcattt
     1021 tgagactgtc ttgagctatg tcccgtatgt cgaaaaactt ctacctttgt cctgcagcgg
     1081 acttaaagag tatttcgata gaaaatatga gcctgatgta aaaacattta tagaccatct
     1141 ggtgccgata tacctgcaca tgaatctttt cagggccttt tcggaatccc ataccagcga
     1201 acaggcggca aggatggtta acatgagttc ggctggcaag aatgcagaag agataataga
     1261 agaccttaca agaatgttca acagaaaacg tcaggcagta attacgcagg agctaagcga
     1321 gattgtggga agtgtaagta tattgaataa gggtgaacta gatggctgaa gtaaacaagg
     1381 gtaaaattgt tcagataatt ggagcggtta tagatataag gtttgagagc gaactacctt
     1441 ctctatacaa tgcgattgaa ataaagaatg gagatgagat tattgtcgct gaggtcatgc
     1501 agcatcttgg cgataatact gtaaggtgca tatccatgca tcctacagat ggtttgagaa
     1561 gaggcatgga cgcagttgac actggcaatc cgatatcagt gcctgttgga gaagaggtat
     1621 tgggcagaat gctcaatgtg tttggtgagc ctattgacaa ccagccggtt gtagaggcaa
     1681 aggaaaaatg ggtaatacac agagaagctc caaagctttc ggaacaggtt gctactcccg
     1741 aagtgttgga aacgggtata aaggcaatag accttctttg tccctttgct aaaggaggaa
     1801 aaataggtct gtttggtggc gccggtgtcg gcaagaccgt actgattatg gaactcataa
     1861 acagtattgc aaaagagcat ggaggttact ccgtatttgt cggagtagga gagcgtaccc
     1921 gtgagggcaa tgatttgtac ttcgatatga aaaactccgg agttcttgac aagactgttt
     1981 tggtattcgg tcagatgaac gagcctccag gagtaagaat gagagttggt ttaaccggtc
     2041 ttaccatggc agaatatttc cgtgaccaga aaaaccagga cgttctcctt ttcatagaca
     2101 acatattccg ttttgtacag gcgggttccg aggtttcggc tctattggga aggacaccaa
     2161 gtgcagttgg ataccagcct acactggcta cggaaattgg cgcattgcag gaaagaattg
     2221 catcaacaaa agcaggttcc ataacatctg ttcaagcaat ctatgtgcct gcggacgact
     2281 ttaccgatcc ggcaacagct accacttttg ctcacctgga tgcagtaacc gttctctcaa
     2341 gaagcatagt ggagatggga atttatcctg cggtggatcc tctgagttca tcctcaagaa
     2401 ttcttgaggc aagcatagta ggttccgagc actacaatgt atcccgttcg gtaaaaagtc
     2461 ttcttcagag atatcaggac ctgcaggata ttgttgcaat acttggtata gatgagttgt
     2521 ccgaagagga caagaagata gtaaacaggg ctagaaaggt acagagattt ttgtcccagc
     2581 ctttctacgt ggctgaaaag tatacacaga tacctggaag atacgttccc atagaggaaa
     2641 cggtaagagg attcagggaa attattgaag gcgttcatga tgatatacca gaaggtttct
     2701 ttctgaatgt aggaacaatt gacgaggttg tcgagagata caagcgttcc atgggtgata
     2761 ttgatggctg aagctgcagc gaaagctgat agaaaaatag atttgaggat aaccacaccc
     2821 agaggtctta agtttgagga aaaagcagac atgattatta tgcgctgcat agacggagac
     2881 atgggtgtgt taagagacca tgctcccatt tctgcagtgt tgggagatgg tatattgaga
     2941 attattaatg atggtgttga gcaaaagctt gccttgttcg gaggaatagt ggagataagc
     3001 gataatacag ttgatattta cagcaccatt gcccagcgtc ctgaagaaat cgacctggag
     3061 cgcgcaagga gagacaagga agaagcggaa gcaatcatca tggaggaaac agaagaactc
     3121 aagattcgca gcatgcaggt cgtactcaga agatcgctgg ttagaatcga ggtcagtctt
     3181 taccttgatg aagagaatga aattagtatt ggtaatacca atgatgacga ggaataaaaa
     3241 agcaggtacc aaagcacctg tttttttatt gtattcttcc atcgtcaata cgtataattc
     3301 tatggcattg ctcgtatatg aaaggatcat gggtggcaat cagaatggtt gccccttcat
     3361 ttttgttcaa gtcatggaaa atatccatca tatcctttga ggttgaacga tcaagatgcc
     3421 ctgtgggttc atcggcaagt atcaagagag gattgttgat tatggctctt gctatggcaa
     3481 ctcgctgctg ctgaccacct gacaaggatt ccaccaggtt ttttttcttt tgatagatgc
     3541 ccagtttctt taagatttca tctattttga gctcaatctc ggaaactttc gttttgtttg
     3601 acagaagcag aggaagagct atattattgt ataccgtatt gcctagtata aggccaaagc
     3661 tttggagcac aaacccaatt tcctcattcc ttattcttgc aatctgtttg gaacctaact
     3721 tggttatatt ccttcctttg aagtaatact ctcccgaatc ataatcatca aggcacccta
     3781 atatgtgaag aagagtactt tttccagaac ctgaaacacc caccagtgca accagctccc
     3841 cttcttctat atttaaagat acatccttta atgcatgaac atcgtttttg ttccctttcc
     3901 tataaacctt atttatattt acaagctcaa tcataattat atatccctcc cgctgattat
     3961 ccctaccgga ctctgttttg aaataattga gagtacaagg aatgacaaaa gcacatgtat
     4021 ggctaaagta atggcaatga atccaaaaaa ggctgttgtg ttgaaaaaca tgtatttaaa
     4081 ttcaaccctg tacaggtatt taatgagaaa gtagcttatt aaccctgaag tcaataccac
     4141 aggtatgttc tgaagaaact gcattgtcaa tgccttcctc ctcgttgcac cgcataaaaa
     4201 cattgtagca aaagtctttg tatatgtatc aatgttcaat attctgtccc ctgtaaatcc
     4261 acaaaggaat attgtataca aggtagatat taatataaca agaaatcctc tgtcactttt
     4321 gtttatttca agaactcttt tgtattcctc atcaaatctc acaataccca attgtgcaag
     4381 ttcgtttgta tcgaagtaat caagccttgc gtatttcata ttgttatact catatctttt
     4441 ttcatgtgga attatcccct tggcatcaag acagattatc tcatcactgt gtatgtatgg
     4501 ttttaaaaga cctgcaacat aaaccgtata ctttgcattt tcatcttcca gagtggtata
     4561 tagtttgtag gttttgccta ccttcaaatc cttcctgaat ttatagggaa taacaacaat
     4621 aaagtcatat tctccaatat caatatcatc aaaccatttg ccaatcttca agggcagctt
     4681 gactatattt agatattctc ttgaatacac tcttgtgttg aacccatctc tcagaggtct
     4741 gtcaggactt tccttgtaga aatataagta tggataattg taatcaatag gattgccatt
     4801 ctttgtgcag tattctactt gctcctcatc ttccatgtcg tagatgacaa tattgtttgc
     4861 aacaagtgct ctaagttcgg ttctgtttgc attgtcaatc aacagatgat tgaaagctat
     4921 atttcccaat acgagcacaa taatcagttc gagaattata attatcctgt tgattgagaa
     4981 tatgctgcca tatttaaaag ccagcatcat atttttaata aatttcatta gagcacctcc
     5041 cttttcatga ctgataaggc agtgggcaga agcagtataa aacagaatac gatgtttgca
     5101 aaaaacgtaa tgtaaacaag gttgggactg agcattagca gggttgtgtc aaactttttg
     5161 aagaagccaa acagaaacag tttgtagatt aatgtgccta aaccaaagga aattgcagtt
     5221 attactattg ggattgtgta catgattg
//
LOCUS       DAONVY010000102         5205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11625_length_5205_cov_3.086019, whole genome shotgun sequence.
ACCESSION   DAONVY010000102 DAONVY010000000
VERSION     DAONVY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_5205_cov_3.086019"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3745
                     /locus_tag="PLI11_03930"
     CDS             <1..3745
                     /locus_tag="PLI11_03930"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52044.1"
                     /translation="CGDIGITRWFGGIEREIVGFFIYVRVRETGEVFSLTYNPVAKKA
                     DKQVVYFYNDRALFKRRDGDLESELEIFVSPEENAEIRRVKFTSNSEQNLHLEVTCVC
                     ELTMTSWMSHQSHPAYSDLMIVTEANPSEYTLIAKRKYKDEPSNLTYACMVASSKSGD
                     ISAFSYDTDFLTFMGRNRDYENPKSMEANAPLSGNHGAVITPCFSMRCMISVPAGESD
                     TLAFTLGYAESRDAIYEIVDKFRNIRNTERALSLAETRCVIEQEYVEVLPGERKAFLS
                     TLSHIVFMSPVRILFSGAISRGQDIVASLWELGISGDNPIVTVFAQKIENYNFLERMI
                     RMWRFFSFRGFVLDLAIITEEGGEYVSSQKDAAESLAAKALAGSYHVRGNIYVIQPME
                     KNRRALECLLAASCIVLKPSSQSQENAKAVAGKEKLYRNMIMAGNEFALRNKEGINYT
                     VPTRDLVFYNGYGGFSKDSYGMYNEYLIDVLYSQNRFITTPRPWINTITGTNFGFIVS
                     ESGSGSVWHINSRENRLTPWYNQLVRDPSGETIYIQNMKNGEYFSATPMPCGHNVLFE
                     VRHGHGYSVFNGKAPGLNTELVVFAAQTKPVKLSLLSITNTTEEDMQLRLVYYVRPVL
                     GDYLSKRGFYTVTSVDAENDALLANNYRVDKFGELTAFISSSLSMDGYTGDDLEFKGI
                     NGTLAAPAALLPENSLSNSCGAGLSPCMAMTSSILIKAGDTFQLVYMLGQTNSDEERV
                     EIIKEFKSTFNAGQELNRVKEYWHNYVTQVVVDTPDKAMNILMNGWLLYQIKACRLEG
                     RTAFYQSGGAFGFRDQLQDCLALLHVDPERVKKQILLHASRQYEEGDVQHWWHEPSGA
                     GIRSRYSDDLLWLPYCVYYYCMHTQDYDILKIEVAYLKSPPLSDAEHDRYEICEVSEL
                     KESLIDHCIRAMKFALKLGKRGLPLMRGGDWNDGMNNVKGESVWLAWFLCTVMDNFKK
                     LCKAAGIYSDHDFVQSIDRITNDIVKSIEEYCWDGNWYVRAFFEDGTPIGSHTSPECS
                     IDSLAQSWSVISGRGDKERAVSALESAVKRLVDFDKGIIKLLTPAFTGEHMNPGYIAA
                     YPPGIRENGAQYTHGSIWLAKALLEFGWYDQGYELLNILNPINHARTQQEVNMYKNEP
                     YAVSADIYTDDKNIGLGGWSFYTGSASWLYKTMLEDFLGITRRGNMLIVNPRIPSSWP
                     GYTIRYRFGNTVYHIEVKNKSKANMESKTIQLVDDGSEHKITI"
     gene            complement(3742..5013)
                     /locus_tag="PLI11_03935"
     CDS             complement(3742..5013)
                     /locus_tag="PLI11_03935"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPZ52045.1"
                     /translation="MNSYAQSMLEYINGSPTPYHAVNKLKDMLDKKNAIGLDVESSWE
                     LEQGKLYYVVNKDTSIAAFYLSSDLEKGFKIAAAHTDSPCFRIKPTSSFIKGGYELLN
                     VEGYGGSILYSWLDRPLGLAGVVYVKEKDSIRKVYINISEPIAIIPSLAIHMNREVNE
                     NVKFNIQNELSPLFAQEDNGQAFISLLAKEANCTEDDVLSFDLMTYDASPGTFVGLNK
                     EFISSPRLDDLSMVYSIFTGFIESCDNMMENAIAVAFDHEECGSLSDRGARSNFLDMI
                     LDRIFESFDMSKEKKYIALFKSFLISGDMAHAAHPAYPDKNDPHMHVCLNKGPVLKHN
                     SNQSYASNAKATAYIKTLCKKNDIPLQEYANRSDVRGGGTIGPMLSAEYGITAVDIGS
                     PMLSMHSARELGGSKDPFMIHKLFKVFYSNN"
BASE COUNT         1557 a          976 c         1217 g         1455 t
ORIGIN      
        1 atgtggcgat attggaataa caagatggtt tggaggtatt gaaagggaga tcgttggttt
       61 ctttatttat gtaagggtta gggaaacagg agaggtgttt tcactgacct ataatcctgt
      121 tgcaaaaaaa gcggataaac aagttgtata tttctacaat gaccgtgcct tgttcaagag
      181 aagagacggg gatttggaaa gtgagcttga gatttttgtg tctcctgaag agaacgcaga
      241 gatcagaagg gtcaagttca caagtaactc cgagcagaac ttgcacctag aggtaacatg
      301 cgtgtgtgag cttaccatga cctcttggat gtcacatcaa tctcatcctg cttactcgga
      361 cctaatgatt gtaaccgagg caaatccaag cgagtacacc cttatagcga aaaggaagta
      421 taaggacgag ccaagcaatc tcacctatgc atgcatggtg gcgtcatcaa aatcaggaga
      481 tatttctgct ttctcttatg atactgattt tttaaccttt atgggaagaa acagagatta
      541 tgagaatcca aaatcaatgg aggcaaatgc gcctctttca ggaaaccatg gtgcggttat
      601 tactccttgt ttttcaatgc gctgcatgat tagcgtacct gcaggcgaga gtgatacact
      661 ggcctttacg ttgggatacg ctgaaagcag agatgcaata tatgaaatag ttgacaagtt
      721 tcgcaacatt agaaacacgg aaagggctct cagccttgct gaaacaagat gcgttattga
      781 acaggaatat gtcgaagtgc tgccgggtga gagaaaggcc ttcctcagta cactttcgca
      841 tattgtcttt atgagccctg tgaggatctt gttttcaggt gcgattagtc gtggtcagga
      901 tattgttgcc agcttgtggg aattgggaat ttcgggtgat aacccgattg ttacagtgtt
      961 tgcacaaaaa atcgagaact acaactttct tgaaagaatg ataaggatgt ggaggttttt
     1021 cagcttcagg ggctttgtgt tagacctggc gataataact gaagaaggtg gcgagtatgt
     1081 aagctctcaa aaggatgctg cagaaagcct tgctgcaaaa gcacttgctg gcagctacca
     1141 tgtgcgtggt aatatttatg taatacagcc catggagaaa aacagacgag cattggaatg
     1201 tttgcttgca gctagctgta tagttcttaa gccatctagt cagagtcagg aaaatgcaaa
     1261 agctgttgct ggaaaggaaa agctttaccg aaacatgatt atggctggaa atgagtttgc
     1321 gctcaggaac aaggagggaa tcaattatac agttccaaca agggatttgg tgttctacaa
     1381 cggatatggc ggtttctcaa aggacagcta tggcatgtac aatgagtatt taattgatgt
     1441 tctgtattct caaaaccgct ttattacaac tccaagacca tggataaaca cgattactgg
     1501 gaccaatttt ggatttattg tttcagagag cggttctgga tcagtttggc atataaacag
     1561 tagggaaaac agattgacac cctggtacaa ccaactggtc agagaccctt caggggaaac
     1621 aatctatatt caaaacatga aaaatgggga gtacttctct gctaccccaa tgccctgtgg
     1681 acacaatgtt ttgtttgagg tgcgccatgg gcatggatat tcagtgttta atggaaaggc
     1741 acctggttta aatacagagc ttgtggtgtt cgctgctcag accaaacccg taaagctttc
     1801 tttgcttagc atcacaaaca caactgagga ggatatgcaa ttgaggctgg tttattacgt
     1861 aagaccggtt cttggagact atctgtccaa gcgcgggttt tacaccgtta cttctgtgga
     1921 cgctgaaaac gatgctcttc tagcaaacaa ttatagagtt gacaagtttg gagaactgac
     1981 ggctttcata agctcatcgt tgtcaatgga cggctatacg ggtgatgatc tggagtttaa
     2041 agggataaac ggaacgttgg ccgcacctgc tgccctgctt ccagaaaaca gcttgtctaa
     2101 ctcatgcggg gctggcttgt caccctgcat ggcgatgaca agcagcatat tgataaaagc
     2161 aggtgatacc ttccaacttg tgtatatgct gggtcaaact aattcagatg aagaacgtgt
     2221 ggagattata aaggagttca agagtacgtt caatgcagga caggaactaa acagagtaaa
     2281 agagtactgg cataattatg taacccaggt tgtagttgat acaccagaca aagccatgaa
     2341 cattctgatg aacggctggc tgttgtatca gatcaaagca tgcaggctgg aaggaagaac
     2401 tgccttctat cagtcgggtg gagcttttgg ttttagagat cagcttcaag attgccttgc
     2461 attgctgcat gtcgaccctg agcgtgtgaa aaagcagata ctgctacatg catcaagaca
     2521 gtatgaggaa ggagatgtac agcactggtg gcatgaacca tccggagcag gtataagaag
     2581 caggtacagt gatgacctct tgtggctgcc ttactgcgtt tactactatt gcatgcacac
     2641 acaggattac gacattctga aaattgaggt tgcatatttg aaaagtcctc cactatccga
     2701 tgcggagcat gacagatatg aaatatgtga agtttcggaa ttaaaggaat ctttgataga
     2761 tcattgcata agagctatga agtttgcatt gaagcttggt aaacgcggtc ttcctctaat
     2821 gagaggcgga gactggaatg atggaatgaa caatgtgaag ggagaaagcg tatggcttgc
     2881 atggttctta tgtaccgtaa tggacaactt caagaaacta tgcaaggctg ctggtattta
     2941 ctctgaccac gattttgttc agtccattga cagaattacc aatgatattg tcaaatctat
     3001 agaagaatac tgttgggacg gcaattggta tgtcagagca tttttcgagg atggcactcc
     3061 tatcggcagc catacatcgc cagagtgttc aatagactcg ttggcgcaat cgtggagtgt
     3121 tatcagtggt agaggagaca aagagcgtgc agtctcagca ttggaatcag cggtcaaaag
     3181 gcttgttgac tttgacaagg ggattataaa gcttttgacg cctgccttta ctggagagca
     3241 catgaatccg ggatatattg cggcatatcc tccgggtatt agagaaaacg gtgctcagta
     3301 tacccatggt tcaatctggc ttgctaaagc cttgctcgaa tttggctggt atgatcaagg
     3361 ttacgaattg ttaaatattc tgaacccgat aaaccatgcc agaacacagc aagaagtgaa
     3421 tatgtataaa aacgagccct atgctgtttc tgcagatata tatacagatg ataaaaacat
     3481 agggcttggt ggatggagtt tttacaccgg ttctgccagc tggctgtaca agacaatgct
     3541 ggaagacttc ctgggaataa caagaagagg aaacatgctc attgttaatc caagaatacc
     3601 gtcatcgtgg ccaggttata caatcaggta cagatttggc aacaccgtat atcatataga
     3661 agtaaagaat aaatctaaag ccaatatgga atcgaaaaca atccagctcg ttgatgacgg
     3721 cagtgaacac aaaataacta tttagttgtt tgagtagaat actttgaaaa gtttgtgtat
     3781 cataaacgga tccttggaac cgccaagttc ccttgcagaa tgcatggaaa gcatgggact
     3841 accaatgtct accgctgtaa ttccatattc agctgatagc ataggtccta ttgttccccc
     3901 gcctcttaca tcgcttctat ttgcatactc ctgcaaggga atatcattct tcttgcaaag
     3961 agtcttaata taagcagttg cttttgcatt ggatgcatag gattggtttg agttgtgttt
     4021 aagcacaggg cccttattca agcaaacatg catgtgtgga tcgtttttat ctgggtaagc
     4081 aggatgtgct gcatgtgcca tgtcgccgga tatgagaaac gatttgaaaa gtgcaatgta
     4141 tttcttctcc ttggacatgt caaagctttc aaaaatcctg tcaagaatca tatcgagaaa
     4201 attggaccta gcacccctgt cactgagtga gccgcattct tcgtggtcga aggctacagc
     4261 gatggcgttt tccatcatat tatcgcatga ctctataaaa ccggtaaaaa tactatatac
     4321 catggacaga tcatcaagtc ttggagagga gataaactcc ttgttcaagc ctacgaatgt
     4381 tccaggagat gcatcgtaag tcattaaatc aaaacttaat acatcatcct cggtacaatt
     4441 tgcttccttt gcaagaagac taataaatgc ctgcccatta tcttcctgcg caaacagcgg
     4501 tgacaattca ttttggatgt tgaacttaac gttttcattt acctccctgt tcatatggat
     4561 agcaagactt ggaattatgg ctatgggttc cgaaatgttt atataaacct ttcggattga
     4621 atctttctct ttaacataga caacaccagc tagtcctagc ggcctgtcaa gccatgagta
     4681 aagtatgctg ccaccgtaac cttcaacatt aagaagttca tagccacctt taatgaatga
     4741 cgatgtcggc ttaattctaa aacaaggcga atctgtatgt gcggcagcaa tcttaaagcc
     4801 tttttccaaa tcactgctta ggtaaaaggc tgcaatggat gtgtctttgt tgacaacata
     4861 atatagtttg ccttgttcaa gttcccagct ggattcaaca tccagcccta tggcattctt
     4921 tttatcaagc atgtccttaa gtttattgac cgcatggtaa ggtgtaggcg atccgtttat
     4981 atattccaac atggattgtg catacgagtt cataaatacc tccgagtaaa atgatatatc
     5041 aagtctagta tacatgaatt ttgctttgtc cacaaacgta aacaatatgt gttaatatta
     5101 ataaactggt ctgagcaaag tctacaaatg tagacaagtt atttgcgtaa agatataata
     5161 ggtaaaaaaa gaataggatg attggattga acattgcagt cgttt
//
LOCUS       DAONVY010000103         5091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11931_length_5091_cov_4.141779, whole genome shotgun sequence.
ACCESSION   DAONVY010000103 DAONVY010000000
VERSION     DAONVY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11931_length_5091_cov_4.141779"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLI11_03940"
     CDS             complement(<1..419)
                     /locus_tag="PLI11_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HPZ52046.1"
                     /translation="MDCASRSKIADVYVENGRVVKLFKEHVPKRRVLGEALNHTIVEE
                     AKINVPELREISLIDGRWALIMDYVEGQTYNDLIFLNPDKKEELVEEFVDIQIEIHVA
                     RVEGLDSLYEKLSSLINSNPELDDVNKYELLARLEGMP"
     gene            complement(511..1647)
                     /locus_tag="PLI11_03945"
     CDS             complement(511..1647)
                     /locus_tag="PLI11_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPZ52047.1"
                     /translation="MVYVQKCDTYDYEVVYEKIKEMFQHLGYDKYLKPELNVCIKPNL
                     VAKKKPEEAATTHPSLVYAVARLFVESGAKVLIAESPGGLYDKKILKGIYKTTGIEEA
                     AEMAGALLNYDLTEAEVECEDNEYIKRMNLITPIVNADLVVNIAKLKTHGMMAYTGSV
                     KNMFGCIAGLKKAEYHFNMPDYDSFANCIIDICLAADPQINIIDAIVGMEKDGPTAGD
                     PKKMDLIIAGDDPFKVDSIAATLIKADIMRIPVLRNAIRRGLCPASHEEVEILGEDLE
                     ECEVKDFIINYHESKSLYFFKGRLGKWLTNIISPKPVFDKKLCKSCGECAKCCPAKVI
                     TVEKGKCAEVDLVHCIRCYCCQELCPFKAVSIKKPLINKLFINS"
     gene            complement(1625..2287)
                     /gene="rdgB"
                     /locus_tag="PLI11_03950"
     CDS             complement(1625..2287)
                     /gene="rdgB"
                     /locus_tag="PLI11_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPZ52048.1"
                     /translation="MLKVVLATQNNNKVREMQSILKDCGVSAEVISAKDAGVHEFPEE
                     NGTTFSENAYIKAKGIFDVVKKSGILQLPYLVIADDSGLCVDALEGRPGIYSARYASV
                     DGNDAADEDNIKKLLMEMEGIPNENRKAYFECSIAAIRDDGKIFSTQGRLHGYISLQP
                     VGCNGFGYDPVFYLPEESCTVAQLDSYKKNRISHRAKAIRSLAEIIKDNMGGYNGICS
                     EM"
     gene            complement(2287..3093)
                     /gene="lepB"
                     /locus_tag="PLI11_03955"
     CDS             complement(2287..3093)
                     /gene="lepB"
                     /locus_tag="PLI11_03955"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPZ52049.1"
                     /translation="MEKENMSSGFESFEEESIISRELENNETVDEGPAEDIGEDAVDE
                     NENTEELAEEEKETVEVKSVTLSYMKVLKSIMSWVWTIVLAFFIAIIINSYVIRASEV
                     FGRSMYPTLEQGDVVFISRMPYIFGKPKHGDIVVFDSTLEKRNFFTEIKESVKYNMIT
                     QKFFKTEKSNKYWIKRVIGVPGDVLEIKDGKLYRNGELLEEDYVNSASPITYKNLVPP
                     SMGDGDLTRIVVKEGEIYCMGDNRNHSEDSRAIGPVPINDVLGKVIYGAK"
     gene            complement(3116..3637)
                     /locus_tag="PLI11_03960"
     CDS             complement(3116..3637)
                     /locus_tag="PLI11_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPZ52050.1"
                     /translation="MNLLLHVCCGPCSLYVIDDIRETFKDCKITGMFANPNIHPYDEF
                     QRRMENAQIALKNKDVPLVCVDDFDMEKWEKYEQDRKSRCKMCYTIRLERVANYAKEK
                     GFTHFTTTLLVSPYQDHEMIKEIGERFAEKNDLIFVYRDFSKGFREGQKQAREMRLYR
                     QKYCGCIKSLYEK"
     gene            complement(3640..4695)
                     /gene="ruvB"
                     /locus_tag="PLI11_03965"
     CDS             complement(3640..4695)
                     /gene="ruvB"
                     /locus_tag="PLI11_03965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPZ52051.1"
                     /translation="MVDFNEEKRIVSPDISADDIDESSLRPKRFGEYIGQEKIKNNLS
                     IFIEAAKQRSEPLDHVLLYGPPGLGKTTLAGIIASEMGVNLRITSGPAIEKAGDLAGI
                     LTNLNEFDVLFIDEIHRLNRTVEEILYPAMEDFAFDIIMGKGPAARSLRIDLPRFTLV
                     GATTRAGLLTAPLRDRFGVISRLEMYTVKELASIVKRSAGILGVKIDDDGAKEIALRS
                     RGTPRIANRLLRRVRDFAQIKGDGDITHEISIKSLDSLEVDKLGLDHVDRNLLSTIIL
                     KFDGGPVGLETLAAATGEEITTIEDVYEPYLIQLGFLAKTPRGRIATHSAYTHLGIPH
                     DHESSQQLEIFDLLKEE"
     gene            complement(4708..>5091)
                     /locus_tag="PLI11_03970"
     CDS             complement(4708..>5091)
                     /locus_tag="PLI11_03970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HPZ52052.1"
                     /translation="MLITVSGVGPRMALNVLSTLTSSELGLAVATEDYKTIAKSKGIG
                     PKLAQRIILELKDKIKKSMDVDQIETAENTGVMIEGNIEKDAISALMVLGYSAKESAS
                     AVRKVYQKGLTLEETVKLALRNMHG"
BASE COUNT         1376 a         1239 c          773 g         1703 t
ORIGIN      
        1 ggcattccct caagacgtgc caataattca tatttattga catcgtcaag ctcggggttt
       61 gaatttataa gggaagacag cttttcataa agactgtcaa gaccctcaac tcttgcaaca
      121 tgaatttcaa tctgaatatc cacaaactct tccacaagtt cctctttttt atctggatta
      181 agaaaaataa ggtcgttgta tgtttgtcct tcgacataat ccataattaa agcccatctg
      241 ccgtctatca atgagatttc acgcagttca ggaacattta ttttggcttc ctcaacaatg
      301 gtgtgattca acgcctcacc aagtaccctt ctttttggca catgttcctt gaagagtttt
      361 acaacccttc cgttttctac ataaacatca gcaatcttgc ttctacttgc acaatccata
      421 gccatcttcc tttccgatta tctctactat attataccat acaaaattct tcgttccaac
      481 aataaaaaaa gagcacacat ccacgtgctc ctaactattg ataaacaact tgttaatcag
      541 aggttttttt atgcttacag ctttaaatgg acacaattcc tgacagcagt agcatctaat
      601 gcaatgaact aagtcaacct cagcgcattt gcccttttca acagtgatta cttttgcggg
      661 gcagcatttt gcacattcgc cgcatgactt gcaaagcttt ttatcaaata caggtttggg
      721 gctaatgata ttcgtaagcc atttgcccag cctgccttta aaaaagtaaa ggctcttaga
      781 ttcatggtag tttattataa aatcctttac ttcacactcc tctaaatcct cccctaaaat
      841 ctcaacttcc tcatgacttg caggacacaa ccccctcctt atggcatttc ttagtacggg
      901 aattctcata atatcagcct tgattagggt tgcagcaatg ctatcaacct taaaaggatc
      961 atcccctgct attatcaagt ccatcttttt aggatcacct gcggtaggtc cgtccttctc
     1021 catgcccact attgcatcaa taatatttat ctgtggatct gcggcaagac aaatgtcgat
     1081 aatacagttt gcaaagctgt catagtcagg catattgaaa tggtattcag ccttcttcaa
     1141 tcctgcaata cagccaaaca tgttctttac acttccagta tacgccatca tgccatgggt
     1201 cttcaatttt gcaatattga caacaaggtc agcattgact ataggggtga taagattcat
     1261 cctctttatg tactcattgt cctcgcattc aacttcagct tcagtcaaat catagttcag
     1321 caaagcacca gccatttcag ctgcttcttc tatccctgta gttttgtaga ttcccttaag
     1381 tattttcttg tcatacaagc ctccaggact ttctgcaatc aaaacctttg caccgctctc
     1441 tacaaacaac cttgccactg catatacaag agatggatgt gttgttgcag cctcctctgg
     1501 tttctttttt gccaccagat taggcttgat gcatacattc aattcgggct ttaaatactt
     1561 gtcataaccc aaatgctgaa acatctcttt tatcttttca tagactactt catagtcata
     1621 tgtatcacat ttctgaacat ataccattat atccacccat gttatcttta ataatttcgg
     1681 caagactccg tatagccttt gccctgtggc ttatcctatt cttcttgtag ctatcaagct
     1741 gtgcaacggt acaactctct tcaggcaaat aaaatacagg atcgtatcca aacccgttgc
     1801 agccaactgg ttgtaaagat atatagccgt gaagtcttcc ttgtgtggaa aatatctttc
     1861 catcgtcacg tattgcagca atggaacatt caaaatatgc ttttctattt tcgtttggaa
     1921 tgccttccat ctccatcaaa agtttcttta tattatcctc atctgcagcg tcatttccat
     1981 ctactgatgc gtatcttgca gaatatattc caggccttcc ttcaagagca tcaacacaga
     2041 gaccagaatc atccgcaatc accaagtatg gtaattgcag tattccgctc tttttaacaa
     2101 catcaaatat tccttttgcc ttgatatatg cgttttcaga aaatgtagtg ccattttcct
     2161 ccggaaactc gtgaacacca gcatcctttg cggatataac ttctgcagaa actccgcaat
     2221 cctttaggat gctttgcatt tcacgaactt tgttattgtt ttgtgtggcc aaaactacct
     2281 taagcattat tttgctccat atataacttt accaagtacg tcgtttattg gaacgggtcc
     2341 tattgctctg ctgtcttcac tgtgatttct attgtcgccc atgcagtaaa tctcgccttc
     2401 tttaaccaca attctggtca aatctccatc acccatggaa ggaggaacaa gatttttgta
     2461 tgttatcggg cttgcactgt tgacataatc ctcttcaaga agttcgccgt ttctgtagag
     2521 ttttccatcc tttatttcaa gtacatctcc cggaactcca ataactctct ttatccaata
     2581 tttatttgac ttttctgttt tgaagaactt ctgggtaatc atgttgtact ttaccgactc
     2641 cttaatttca gtaaagaaat ttcttttttc cagagtagaa tcaaatacaa ctatatctcc
     2701 atgtttgggc ttgccaaaga tgtacggcat tctggaaatg aaaaccacat ccccctgttc
     2761 cagagttgga tacatggacc ttccaaatac ttcgcttgcc ctaattacat atgaattgat
     2821 aattatagct atgaaaaatg caagaacaat tgtccatacc cagctcatta tgcttttcaa
     2881 cactttcatg tacgaaagtg taaccgattt aacttcaacc gtctcttttt cctcttccgc
     2941 caactcttcc gtattttcat tttcatcaac ggcatcttca ccaatatctt cggctggacc
     3001 ttcatctacc gtttcgttat tttccaattc tctggagatt attgactctt cctcaaaaga
     3061 ctcaaatccg cttgacatat tctctttctc cataacattt tcctttcatc acaaactatt
     3121 tttcatacaa agattttata caaccacagt atttttgtct atacaatctc atttcccggg
     3181 cctgtttctg accttctcta aaacctttgg aaaaatccct gtatacaaat atcaaatcgt
     3241 ttttttctgc aaatctttct ccaatttcct taatcatctc atggtcctga taaggactaa
     3301 ccaaaagcgt agtggtgaaa tgggtaaaac ccttttcctt tgcataattt gctacccttt
     3361 ctagtcttat agtatagcac attttgcacc ttgattttct atcctgctca tatttttccc
     3421 atttttccat atcaaaatca tccacacaaa caagaggtac atccttgttt ttcaaagcta
     3481 tctgagcatt ctccatcctt ctttggaatt catcataagg gtgaatattt ggatttgcaa
     3541 acatgcctgt aattttgcaa tccttgaaag tttcccttat gtcatctata acatacaggc
     3601 tgcaaggtcc gcagcataca tgtaaaagca ggttcaattt tattcctcct tgagcaaatc
     3661 aaaaatttca agctgttgac tgctttcatg atcatgagga atcccaagat gagtatacgc
     3721 agaatgtgtc gcaatccttc ctcttggtgt ctttgcaaga aatccaagct gaataagata
     3781 aggttcgtat acatcctcaa ttgttgtaat ttcttcaccc gttgcggcag ccaacgtctc
     3841 cagtcctaca ggacctccat caaactttaa tattatcgta cttaaaagat ttctatccac
     3901 atgatctaga cccagcttgt cgacttcaag tgaatccaga ctctttattg aaatttcgtg
     3961 cgttatgtct ccatcgcctt ttatctgagc aaagtctcta actcttctaa gcagtctgtt
     4021 tgctattctt ggagtacccc ttgacctcaa agcaatttct tttgccccat catcatctat
     4081 tttaacacca agaataccgg cagatctttt aactatagag gctagctcct tcacggtata
     4141 catctccagc ctgcttataa caccgaacct gtctcttaat ggtgcagtaa gcaaacctgc
     4201 ccttgttgtt gctcctacca atgtaaatct tgggagatca attctcaatg accttgcagc
     4261 aggtcctttg cccataataa tatcgaaagc aaagtcctcc atggcaggat aaagaatctc
     4321 ctcaactgtt ctgttcaatc tgtgaatctc gtcaatgaac aaaacatcaa actcattcag
     4381 gtttgtaagt atgccggcca aatccccagc cttctctatg gcaggccctg atgtaattct
     4441 taagtttaca cccatttcac ttgcaataat gccagccagg gtggtttttc caagtccagg
     4501 cggtccgtaa agcagcacat gatcaagagg ttcgcttctt tgtttagcag cttcaatgaa
     4561 tatggaaaga ttatttttta tcttctcctg tcctatatat tcaccaaacc tcttcggcct
     4621 aagactgctt tcatcaatgt catcagctga aatgtccgga gaaactattc ttttttcctc
     4681 attaaaatca accattgtgc ctccctatca accatgcatg ttccttaatg ccagtttgac
     4741 cgtctcttca agagtcagac ctttctgata aacttttctt actgcgctgg cagattcttt
     4801 tgcactgtaa ccaagaacca tcaatgcaga aattgcatcc ttttcaatat tgccctctat
     4861 catgacaccg gtgttttctg ctgtttcgat ttggtccaca tccatgcttt tctttatctt
     4921 gtctttcagt tccagtatga tcctctgagc cagttttgga cctatgcctt tactctttgc
     4981 aatggtcttg taatcttctg tcgccacagc aagaccaagc tcggaagatg taagggtcga
     5041 aagcacattg agagccatcc tgggaccaac tccactaacg gttataagca t
//
LOCUS       DAONVY010000104         5073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11968_length_5073_cov_4.161618, whole genome shotgun sequence.
ACCESSION   DAONVY010000104 DAONVY010000000
VERSION     DAONVY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11968_length_5073_cov_4.161618"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..440
                     /gene="rpmA"
                     /locus_tag="PLI11_03975"
     CDS             162..440
                     /gene="rpmA"
                     /locus_tag="PLI11_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPZ52053.1"
                     /translation="MLRINLQLFAQKKGVGSSKNGRDSESKRLGVKRADGQFVLAGNI
                     LVRQRGTRIHPGANVGMGKDHTLYALVDGKVSFHRLGKKKKQVSIIAE"
     gene            508..1824
                     /gene="obgE"
                     /locus_tag="PLI11_03980"
     CDS             508..1824
                     /gene="obgE"
                     /locus_tag="PLI11_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPZ52054.1"
                     /translation="MFIDKAKIIIKSGDGGDGIVSFRREKYVPAGGPDGGNGGDGGNV
                     IFKVDEGASTLANFRYKRKYKAGNGQNGGPNKRTGKKGEDIVIKVPLGTVVYDRHTNR
                     VLADMVSSDDEVVVLKGGKGGRGNMNFATSTRQVPNFAKAGEKGEELEVVLELKLLAD
                     VGLAGWPNVGKSTLLAATTDAKPEIANYHFTTINPILGVVTYEDKSYVMADIPGLIEG
                     ASEGVGLGHQFLRHIERTRLIIHVIDMAGTEGRDPFEDFLMINNELEKYNEELAKRPQ
                     IIAANKMDVAGSDENLVVFREKLEKWIEENDRGKQEETGAFRIFEISALKQIGTRELV
                     MYAGSLLEKLPQIPLVSFEEEVIVHKAVEEEDEFAIHVDEEGKYVVEGSFVDNLLRGI
                     NFNDYESMAYFQNSLRRKGVIDELKDMGINEGDIVKLDDIEFEYIE"
     gene            1936..2127
                     /locus_tag="PLI11_03985"
     CDS             1936..2127
                     /locus_tag="PLI11_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52055.1"
                     /translation="MECYGCGNCKQGKLTYYCLMKDEFVVSDESKNLVVEKSRSGRWK
                     KGAPEYEGHRRKSRKIDVV"
     gene            complement(2133..2663)
                     /locus_tag="PLI11_03990"
     CDS             complement(2133..2663)
                     /locus_tag="PLI11_03990"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HPZ52056.1"
                     /translation="MFENIEQQTENKIILLFILEKFGSPITGMYLTDLVLKPGLMNYF
                     SCQMALIDLVENDLVDTTPDNDGIPMYEINDKGRDILISLRNIVPPGILYRYEECIEN
                     EKDDIKRQLEVNASFFEDASGDYYVRCFVRNQGTYIIDIKVPVANKKEADLICKNWHQ
                     NTANIYTKILKVMHDG"
     gene            complement(2682..3053)
                     /locus_tag="PLI11_03995"
     CDS             complement(2682..3053)
                     /locus_tag="PLI11_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HPZ52057.1"
                     /translation="MSNEAYGKRIWLIPDGYYPEVSKGDVYVSHEAVCVLNVGNEDAN
                     IDITLYFEDREPMTGFKAVCKAQRTNHIRMDKIVSVNGEKVPRGVPYAMMVVSDVPVV
                     VQYSRLDASQAEMGFMGLIAY"
     gene            3160..4422
                     /gene="rhaA"
                     /locus_tag="PLI11_04000"
     CDS             3160..4422
                     /gene="rhaA"
                     /locus_tag="PLI11_04000"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HPZ52058.1"
                     /translation="MKQMSYVKESYEIAKKRYAEIGIDTDKAIAKLKEIPISMHCWQG
                     DDVGGFENPEAGLSGGIQATGNYPGKARTADELRADIEKAFSLIPGKHRVNLHAIYGD
                     FETVVERDKIEPEHFEKWVQWAKKLGIGLDFNPTCFSHPLSDDGLTISHPKKEVRDFW
                     IRHCKAARAIGAYFGKELGTPSVVNYWFPDGMKDITVDKYAPRKRLMEALDEVFSDDL
                     PKEYVRDAVESKLFGIGAESFTVGSNEFYMGYAATRNKLLCLDAGHFHPTEVISDKLS
                     AVFCFVDEVLLHVSRPVRWDSDHVIVLNDELRNIAEELVRNDFLGRTFIGLDFFDASI
                     NRIAAWVIGMRNMQKALLLAMLEPTEELKKLETEFDFTSRLALLEELKTMPFGAVWDY
                     FCEINDVPVGDKWLEEVKNYEQEVLLKR"
     gene            4638..>5073
                     /locus_tag="PLI11_04005"
     CDS             4638..>5073
                     /locus_tag="PLI11_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPZ52059.1"
                     /translation="MTKYDPAKIEFKWQEIWEKNKAFEAKDDTTKPKFYALIEFPYPS
                     GDGLHVGHPRSNTAMDIISRKRRMEGYNVLFPIGWDAFGLPTENFAIKNKVHPAVVTE
                     RNVNRFREQLKRLGFSFDWSREVNTTDPAYYKWTQWIFLKLYE"
BASE COUNT         1618 a          864 c         1179 g         1412 t
ORIGIN      
        1 atcggatggt tacatgaagt ttacccggcc gataagcacc aattcgaaag agattattaa
       61 agataatgcg gttttggaag caatggtctt gggttttaaa caggttgaat actcatatgg
      121 cagcaaattt gtacgtgtta aagaagagga ggtatagtcg catgttgaga ataaatctac
      181 agttgtttgc acagaaaaaa ggtgttggca gctcaaagaa cggaagagat tccgaatcca
      241 agagactcgg agtaaagcgt gcagacggac agttcgtttt ggcaggaaac atacttgtca
      301 gacagagggg aaccagaatt catcctggtg ccaacgttgg aatgggcaag gaccatacgc
      361 tttatgcact ggttgatgga aaagtaagtt ttcatagact tggcaaaaag aagaagcaag
      421 tttccatcat tgctgaataa taatatgtaa tgttcaagct aaagaaagtc cggttttacc
      481 gggcttttta tgtaggagga tataattatg tttatagata aggccaaaat tattatcaaa
      541 tcaggagacg gcggagacgg tattgtttcc ttcagaaggg agaaatacgt acctgcaggt
      601 ggtcccgatg gcggcaacgg cggggatgga ggaaatgtca tcttcaaggt tgacgaagga
      661 gcctcaactc ttgccaattt tagatacaaa agaaaataca aagccggtaa tggacagaat
      721 ggaggaccga ataaaaggac gggtaaaaag ggagaggata ttgtaatcaa agttcctctt
      781 ggcacagttg tatatgacag acatacaaac agagttcttg ccgacatggt gtcttccgat
      841 gatgaagtag ttgtgctgaa gggtggaaaa ggcggcagag gcaacatgaa ctttgcaact
      901 tccacaaggc aggttccgaa ctttgcaaaa gcaggagaga aaggtgagga gcttgaggtt
      961 gtgcttgagc ttaaacttct tgcagatgtt ggccttgctg gatggcccaa tgtaggcaag
     1021 tcaacacttt tggccgctac aacggacgca aagccagaga ttgcaaatta tcattttacg
     1081 acgattaatc ctatcctggg tgttgtcacg tatgaggaca aaagttacgt tatggctgac
     1141 attccaggtc tcatagaagg agcttctgaa ggcgtgggtc ttgggcatca gttcttgagg
     1201 catatagagc gtacaaggct cattatccat gtgatagaca tggcaggcac tgaaggaagg
     1261 gatccttttg aagattttct tatgattaac aacgagcttg aaaagtacaa tgaggaactg
     1321 gcgaaaaggc cgcaaattat tgctgcaaac aaaatggatg tggcaggcag tgatgagaac
     1381 cttgttgtgt tcagggaaaa acttgaaaaa tggatagagg aaaacgatag agggaagcag
     1441 gaagagacgg gtgctttcag aatttttgaa atatctgcat tgaagcagat tggaacacgt
     1501 gagcttgtaa tgtatgcagg ttcgcttctg gaaaagctgc cacaaattcc tcttgtatcc
     1561 tttgaagagg aagttatcgt tcacaaagcc gtggaagaag aggatgagtt tgccatacat
     1621 gtagacgagg aaggcaaata cgtggttgaa ggtagttttg tcgataatct gttaagaggt
     1681 atcaatttca acgattatga atccatggcg tattttcaga attccctgag aagaaagggt
     1741 gttattgatg agctcaaaga tatgggaatc aatgaagggg atattgtcaa actggatgat
     1801 attgagtttg agtatataga ataattttcc ttcatattaa attaatattt atttaaaaag
     1861 actattgatt tttaaaatac aatgtattaa aatacctcca tcataaaaat gaaatggagg
     1921 atttttttta gctttatgga atgctatggt tgtggcaatt gcaagcaagg taagttaacc
     1981 tactattgcc ttatgaagga tgaatttgta gtaagtgatg aaagcaagaa tctcgtagtt
     2041 gaaaagagca ggtcaggaag gtggaaaaaa ggagctccgg aatatgaggg tcaccgcagg
     2101 aaatcaagga aaatcgacgt agtctgattt tgtcatccat catgcattac ttttagtatt
     2161 ttagtgtaaa tatttgcggt gttttgatgc cagtttttgc agatgaggtc agcttccttc
     2221 ttgtttgcaa caggaacctt tatatcaatt atatatgttc cctgattgcg cacaaaacat
     2281 cgcacataat aatctccgga agcatcttcg aaaaaggatg cgtttacttc taattgtctt
     2341 ttgatgtcat ctttttcatt ttcaatgcat tcttcgtatc tgtacagaat accagggggg
     2401 actatgtttc ttaaagaaat tagtatatcc cttcctttgt catttatttc atacatggga
     2461 atcccatcgt tgtcgggtgt agtatcaaca aggtcgtttt caaccagatc aataagcgcc
     2521 atttgacagg aaaaataatt catcagacct ggctttaaaa ccaggtcggt taaatacatt
     2581 cctgtaatgg gcgagccgaa cttctccaaa ataaatagca gtataatctt gttttctgtt
     2641 tgctgttcaa tgttttcgaa cataccaaag ccctttcgtc cttaatatgc tataagtccc
     2701 atgaagccca tttcagcctg agatgcatca agtctgctgt actgtaccac aacagggaca
     2761 tcacttacca ccatcattgc gtaaggaact cctcttggaa ccttttcgcc gttaacgcta
     2821 acaatcttgt ccattcttat gtgattggtt ctttgagcct tgcaaacagc tttgaaaccg
     2881 gtcatgggtt cacgatcttc gaagtagagg gtaatatcaa tatttgcatc ctcattgcca
     2941 acattaagta cacaaactgc ctcatggctt acataaacat cacctttgct gacctcgggg
     3001 tagtatccgt cgggaatcag ccatatgcgc tttccatatg cctcgttact catagcaaac
     3061 ctcccaaaat ttagtttaca tatttcatat tatatagtaa actaatataa gcagaatgtc
     3121 aaagcaaaaa ttctgtgcgc aaaacatatc gagaggagaa ttaagcagat gagttatgta
     3181 aaggaaagct atgaaattgc aaagaaaagg tatgctgaga ttggcatcga cacagacaaa
     3241 gctattgcca aactcaagga aatacccatc tcaatgcatt gctggcaggg agacgacgta
     3301 ggcggatttg aaaatcccga agcaggactt tcaggaggca tccaggctac tggcaactat
     3361 ccgggcaagg caaggactgc tgacgagtta agagcagaca tagaaaaagc tttttcatta
     3421 attccaggaa aacatagagt taacttgcac gccatatacg gcgattttga aacagttgtt
     3481 gaaagagaca aaattgagcc cgagcatttt gagaaatggg ttcagtgggc aaagaaactt
     3541 ggtataggac ttgatttcaa ccctacatgc ttctcacatc cattgtcaga tgacggcctt
     3601 acaataagcc atccaaagaa ggaagttaga gatttctgga ttcgccactg caaagctgca
     3661 agagctattg gtgcatactt tggaaaagag cttggaactc caagcgttgt caactactgg
     3721 ttccctgatg gaatgaagga cataactgtt gataaatatg ctccaagaaa acgcttgatg
     3781 gaagctttgg atgaggtatt cagcgatgat cttccaaaag agtatgttcg tgatgcggta
     3841 gaaagcaagc tttttggtat tggtgctgag agctttacag taggatccaa tgaattttac
     3901 atgggatatg ccgctacaag aaacaagctc ctttgtcttg atgcaggtca cttccatcca
     3961 acagaagtga tatctgacaa gctgtcagct gttttctgct ttgttgacga agttctgttg
     4021 catgtaagcc gtcctgtaag atgggatagc gatcatgtta ttgtacttaa tgacgagctt
     4081 agaaatatag cagaggaact tgtaaggaat gacttccttg gaagaacatt tattggtctt
     4141 gacttctttg atgcaagcat caacagaatt gcagcatggg taattggtat gagaaacatg
     4201 cagaaggctc tcttgcttgc aatgcttgag cctacagagg aactcaagaa acttgagact
     4261 gaatttgatt tcacatccag actcgcactt cttgaagagc ttaagacaat gccatttggt
     4321 gctgtttggg attatttctg cgaaatcaac gacgttcctg taggagacaa gtggcttgaa
     4381 gaagttaaga actatgagca ggaagtatta ttaaagagat aataattggt gtatatatgt
     4441 gagaaaggct atggttgcaa aactgtagcc tttttatttt gagtattctc ctgatgagac
     4501 accgtatttt atgaagatat aattgtgtag tgtgaatcaa taaatacttg acaaaaatga
     4561 catctgcagt ttgattagac aaaaagtata tgttataatt accgggtata cgtatacaaa
     4621 atttaggagg tctttgcatg acaaaatatg atccggctaa aatagaattt aaatggcagg
     4681 aaatatggga aaagaacaaa gcatttgaag caaaagatga tacgacaaag ccaaagtttt
     4741 atgcacttat agagtttcct tatccttcgg gggacggact tcatgttggt cacccaaggt
     4801 ccaatactgc aatggatata atttcaagaa agagaagaat ggaagggtat aacgtactgt
     4861 tcccaatagg atgggacgct ttcggacttc caacagagaa ctttgctata aagaacaagg
     4921 ttcatcctgc agtagtaaca gagagaaacg taaatagatt cagagagcag ctcaagagac
     4981 ttggattttc atttgattgg tcacgagaag taaacacaac tgatccggca tattataaat
     5041 ggacccagtg gatctttttg aaactgtatg aac
//
LOCUS       DAONVY010000105         5068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11985_length_5068_cov_3.138041, whole genome shotgun sequence.
ACCESSION   DAONVY010000105 DAONVY010000000
VERSION     DAONVY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11985_length_5068_cov_3.138041"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PLI11_04010"
     CDS             complement(<1..1041)
                     /locus_tag="PLI11_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPZ52060.1"
                     /translation="MLLLGNEAVARGAWEAGVKVVSSYPGTPSTEITESIAKYEDVYC
                     EWAPNEKVALEVAIGASIAGARAMCCMKHVGLNVAADPLFTSSYTGVNGGLVIVVADD
                     PGMHSSQNEQDSRFYALASHVPMLEPADSEECRLFTKLAFEISEEYDCPVILRLTTRI
                     SHARSLVNTQDRESIVNKEYVKDIMKYVMMPGMAKKRHLVVEDRMNRLAAYSNKSGVN
                     RIEWNDKKVGVITSGILYQYVKEVFKDASVLKIGMVHPLPLDLIKQFAEEVEELVVIE
                     ELEPFIENHVRQMGVKVKGKDLTGRQGELSTTILRKQFLNEEISYADVDNEFDIPNRP
                     PVMCPGCPHRAVF"
     gene            complement(1065..2603)
                     /locus_tag="PLI11_04015"
     CDS             complement(1065..2603)
                     /locus_tag="PLI11_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845716.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPZ52061.1"
                     /translation="MVVKILLLAVFFTVTTVIGLISRKKVMSVNDFVLGGRNIGAWLG
                     AFSYGTAYFSAVVFVGYAGQFGWSYGVAAVWIGLGNAFVGSLMAWALLGNRTRTMTKH
                     LDTATMPEFFEKRYGSKAIKLAAAIIMFVFLIPYSASVYKGLSGIFTKAFNIDFGWCV
                     VGMAVLTAIYVVLGGYIASSINDFIQGIIMLFGIVAVIVSVLNGKGGFAESMTLLSQQ
                     EVTSGANAGMNGGYVSFFGPEPFGLLGVVILTSLGPWGLPHMIHKFYSIRDAKEIRKG
                     TVISTLFALIIAGGSYFMGAFGRLYVESDANGMPVTGSDGVVPAMLSSILPDILLGVI
                     VVLVLSASMSTLSSLVISSSSTFAIDFVKGFLYKKIDDKKQMLVIRIMSAFFILVSVL
                     IALFSNELISGLMSISWGALAGAFLAPFLYGLFWKGITRAGVWAGFITGVAVTVANIF
                     TQTFIAPVAGALAMFASLIVVPVVSLVTPKLEKERIDKCFECFNGNMGEEESEYLKGV
                     SVAS"
     gene            complement(2694..>5068)
                     /locus_tag="PLI11_04020"
     CDS             complement(2694..>5068)
                     /locus_tag="PLI11_04020"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPZ52062.1"
                     /translation="DFGVEKGMPGFFTDEPQYYRYKTPWSNILRDEFNKKYGYDILNF
                     LPALFVTFDEEGLDWKKIRYDYHYLVHELYTNNFIKVIYDWCEENNCLLTGHTVEEAR
                     LSTQMWCCGGAMPFYEYQHIPGIDHLGRGIPADIAPKQVGSVAAQLGRKKVLSEMFGC
                     CGWDVSPYELKKIAEWQYASGVNLMCQHLYPYSIRGQRKRDYPAFYSEHLPWQEQMTD
                     FNVYFKRLGYVLAMGSELVNTLIIHPIHSAYLTYNRQKDMESVMELEKEFEYITNMFS
                     QNQIAYHYGDEILMAKHAKVNDDGTLSVGLCTYEYVVLPYCQTLDSSTVELLKEFLEK
                     GGKLYLYKGLPERIDGRKADLSFLKQTCTFEDIKDAQIIKLSKDGNNIPYLRVMTRDT
                     EDGRIFYILNLSDEVIKDVDVEIKGRKEISVLELMDLKLKEVAGSKLSFEGGESFVLV
                     EKASDVKADMEVREFAKSKVEGRFVFKDKVVNAINLDYAYMSYDGETYEKLMPVMQIK
                     DLLFRRKYEGKLYLKYEFNVMEMPGALRACFEPLKYSSVKVNGMDVVVSDEWFLDRSF
                     LVADILEFVKAGKNELVFELDYYQDDYVYYVLYGGVSESLRNCLNFDTEIESVYLYGD
                     FSVVCDSDFTSPRKNVLEYTGQFHIARQKEDVDISDVVRDGYPFFSGKMHVAKTFNID
                     KVQKIELIVDGRYSVCDCYVNDKKAGRLMYKNHCDITEYIKQGENELELVFYNSQRNL
                     LGPHHFADPEPTALGPVQFSLENMWTEDGSHAHRDRYSFVRFGADIYIGT"
BASE COUNT         1505 a         1291 c          802 g         1470 t
ORIGIN      
        1 aaaaacagcc ctgtgaggac atcccggaca cattacagga ggtctgtttg gaatatcaaa
       61 ttcattatcc acatccgcat acgagatttc ttcattcaag aactgctttc tcaatattgt
      121 tgttgaaagc tctccttgtc ttccagtaag atccttgcct tttaccttaa cacccatctg
      181 ccttacatgg ttctctataa atggttcaag ctcctcaatt actacaagct cctcaacctc
      241 ttctgcaaac tgtttaatca aatccagcgg aagaggatga accatgccta tcttcaaaac
      301 tgacgcatcc ttgaaaactt ccttcacata ttgatacaat atcccagatg taattactcc
      361 aacttttttg tcattccact caatcctgtt aacacccgac ttgtttgaat atgcagccag
      421 cctgttcatt ctgtcttcca caaccaaatg gcgctttttt gccattccag gcatcatgac
      481 atacttcata atgtctttta catactcttt attaacaata ctctctctat cctgtgtgtt
      541 taccaggctt cttgcatgtg aaatcctagt agtcagtcgg agaattacag gacaatcgta
      601 ctcttcactt atctcaaaag ccagttttgt aaataatctg cactcttcac tgtctgcagg
      661 ttcaagcatc ggcacatggc ttgcaagtgc ataaaacctg gaatcctgtt cgttctgcga
      721 tgaatgcatg cctggatcgt ctgccaccac tataaccaat cccccattca cacctgtgta
      781 ggaagatgta aacaaaggat ccgcagcgac attaagccct acatgcttca tgcagcacat
      841 cgctctagca ccggcaatgg atgcgcctat tgccacttca agagcaacct tctcgtttgg
      901 agcccattcg cagtaaacat cctcgtactt tgcaatactc tcggtaatct cagtacttgg
      961 tgttcctggg taggacgata cgacctttac accggcctcc caggctcctc ttgcaactgc
     1021 ttcatttccc aacaacaaca tataaaatct ccttcaatct aagtttatga tgcaacagat
     1081 acccctttca ggtattctga ttcttcctct cccatatttc cattaaaaca ttcaaaacac
     1141 ttgtcaattc gttccttctc aagttttggt gtaacgaggc ttaccaccgg aactactatc
     1201 aatgatgcaa acattgccaa tgcacctgcc acaggtgcta tgaatgtctg cgtaaaaatg
     1261 tttgcaaccg taactgcaac cccagttata aatccagccc agacgcctgc tcttgttatg
     1321 cctttccaga acagtccata aaggaagggt gcaagaaacg ctccagccaa agctccccag
     1381 gagatggaca ttaatcctga tataagttca ttactgaaca atgcaatgag aactgaaacc
     1441 aggataaaaa aagcactcat aatacgaatt acaagcatct gcttcttatc gtcaatcttc
     1501 ttgtacagaa agcctttgac aaaatcaatg gcaaaggttg acgaagaaga tatgacaagg
     1561 gaagacagtg tcgacattga agcggacaaa accagaacca caattactcc cagcagtatg
     1621 tcaggcagta tcgaggacaa catggcaggt actacgccgt ctgatcctgt tactggcatg
     1681 ccatttgcat cactttcaac gtaaagcctt ccgaaagctc ccataaagta cgaaccacct
     1741 gctattatca acgcaaacaa tgtggatatg acggttcctt tacggatttc ctttgcgtcc
     1801 cttatggaat aaaacttatg tatcatatgt ggcaatcccc acggacctag acttgtaaga
     1861 attactactc ccaaaagtcc aaaaggttca ggtccaaaaa aagatacata ccctccattc
     1921 attcctgcat tagctcctga tgttacttct tgctgcgaca aaagcgtcat tgattctgca
     1981 aagccacctt ttccattcaa tacagataca attaccgcaa caataccaaa cagcataatg
     2041 attccctgaa tgaaatcatt aatggatgag gctatgtatc ctcccaatac cacataaatc
     2101 gcagtcaata cagccatgcc gacaacacac caaccaaagt caatgttgaa ggcttttgta
     2161 aatatgcctg atagcccttt gtaaactgaa gccgaataag gaattagaaa aacaaacata
     2221 ataattgcgg cagccagttt tatggctttg cttccatacc tcttctcaaa aaactcaggc
     2281 attgtggccg tatccagatg tttcgtcatg gtccttgtcc tgttacctaa aagcgcccag
     2341 gccattagac ttcctacaaa tgcgtttcca aggccaatcc ataccgcagc aactccatac
     2401 gaccagccaa actgtcctgc ataacctaca aatactactg ctgaaaaata cgccgttccg
     2461 tacgaaaatg ctcccaacca tgctccaata tttctgccac ccaatacaaa atcatttact
     2521 gacatgactt tttttcttga tatcagccca attactgtcg taacagtaaa aaaaacagcc
     2581 agcagcaaga tttttactac catactacca tcctccacaa atatattaac aataaaaaaa
     2641 gacgcaatga acaatcattg cgttttaatt atacagaatt gcattattaa atgtcaagtt
     2701 ccaatgtaaa tatctgcacc aaaccttaca aaggagtatc tgtccctgtg agcatggctt
     2761 ccatcttccg tccacatatt ctcaagggag aattgaacag gacccaatgc ggtgggttcg
     2821 ggatctgcaa aatgatgtgg tcccagaaga tttctttgtg agttgtagaa aaccagctcc
     2881 aactcattct ctccctgctt gatatactcc gtaatatcac agtggttctt gtacatgagc
     2941 ctgcctgcct ttttgtcatt tacatagcag tcacaaacgg aatatcttcc atccactatc
     3001 aattcaatct tctgaacctt gtcaatatta aaagtctttg caacatgcat ttttcctgag
     3061 aagaatggat agccgtctct taccacatcg gaaatatcca catcctcctt ctgtcttgca
     3121 atatggaact gaccagtata ctcaagaaca ttctttcttg ggcttgtaaa atcactgtca
     3181 caaacaacag aaaagtctcc ataaaggtat acactttcaa tttccgtgtc aaagttcaaa
     3241 cagtttctca aggactccga tacccctcca taaagaacgt aatatacata gtcatcctgg
     3301 tagtaatcca gctcgaaaac aagttcgttc tttcctgcct tcacaaactc caatatgtct
     3361 gcaaccagga agcttctgtc aaggaaccac tcatcagaca ccactacatc cataccattt
     3421 accttgacag acgaatattt caatggctca aaacaagccc ttaaagcacc cggcatctcc
     3481 attacattaa actcatattt taaatacagc tttccttcat attttctcct gaaaagaaga
     3541 tcctttatct gcattacagg cataagcttt tcataagttt ctccatcgta tgacatatat
     3601 gcatagtcca ggtttatggc atttacaact ttatccttga agacaaacct gccttcaacc
     3661 ttactttttg caaactcccg tacctccata tccgccttta catcacttgc cttttcgaca
     3721 agcacaaaag actcaccgcc ttcaaaactc agcttgctgc ctgcaacttc cttcaatttc
     3781 aaatccatca gttcaagaac ggatatttcc tttctgcctt taatttcaac atcaacatcc
     3841 ttgattacct catccgacag attaaggatg tagaaaattc ttccgtcctc tgtatccctt
     3901 gtcatcacac gaagataagg aatattgttt ccatcctttg ataacttgat tatctgcgca
     3961 tccttaatat cctcaaaagt gcatgtttgt ttaaggaaac tcaaatccgc ctttcttcca
     4021 tcaattcttt caggaagacc tttgtacaga tagagttttc ctcccttttc gagaaactcc
     4081 tttagaagtt ccacagtgct tgaatccaga gtttgacaat atggcagcac tacatactca
     4141 taggtacaca atcctacgct caaagtacca tcatcattta ctttcgcatg ctttgccata
     4201 agaatttcat caccgtaatg gtatgcaatc tggttttgtg aaaacatatt tgtaatgtat
     4261 tcgaactctt tttcgagctc cattactgat tccatatcct tttgcctgtt gtatgtgaga
     4321 tatgcactgt gtatggggtg aataatcagt gtgtttacca attcactacc cattgcaaga
     4381 acgtaaccaa gacgcttgaa ataaacattg aaatcagtca tctgttcctg ccatggaagg
     4441 tgttctgaat aaaaagctgg ataatccctc tttctctgtc ctcttatgga ataaggataa
     4501 aggtgctggc acataagatt tacaccactg gcatactgcc attcagctat tttcttaagc
     4561 tcataaggag atacatccca gccacagcat ccaaacatct cgctgagcac tttctttctg
     4621 cccaactggg cagcaacgga acctacttgc tttggagcaa tgtcagcggg tattcctctt
     4681 ccaagatggt caataccagg tatatgctgg tactcataga aaggcatggc tcctccgcaa
     4741 caccacatct gggtcgacag ccttgcctcc tcaacagtgt gtcccgtcaa aaggcaattg
     4801 ttctcctcgc accagtcata tattacttta ataaaattgt tggtatacag ctcgtgcaca
     4861 aggtaatggt agtcgtacct tatcttcttc caatcaagac cttcttcgtc aaatgtaaca
     4921 aaaagggctg gcaaaaagtt caatatgtca taaccatact tcttattaaa ctcatccctt
     4981 agaatatttg accaaggtgt tttatacctg taatactggg gttcgtccgt aaagaatccg
     5041 ggcatgccct tttctactcc aaaatctt
//
LOCUS       DAONVY010000106         5061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_11997_length_5061_cov_3.347383, whole genome shotgun sequence.
ACCESSION   DAONVY010000106 DAONVY010000000
VERSION     DAONVY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11997_length_5061_cov_3.347383"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLI11_04025"
     CDS             <1..564
                     /locus_tag="PLI11_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ52063.1"
                     /translation="KYDGETKTELSLDGAATDASSTNNPSSNAIDGSNNTKWSSVGNS
                     VPQWIQVILPEVKNLSGFRLVADFNWTDIRAYHVEVATKDGWVKVYEHSGESIEHFQE
                     IDFDTLWNIDSIRFTITEVGPDALQGHGTTSIDILEIKLFTADAIEQETEPETNPQTS
                     DMSIIFILGMTAVLGLSLFKRRMTVNA"
     gene            complement(609..1703)
                     /locus_tag="PLI11_04030"
     CDS             complement(609..1703)
                     /locus_tag="PLI11_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52064.1"
                     /translation="MLDYIVVSYLKKNVIDMLKNDLDKIILPWTEQEHICKEYLERKN
                     DPDYMRTFDKKRCYDLLLSLPGITDDMPRPTIIYDFFEDDIYINRHGRYDIPFKHRHT
                     FYEMLFVYEGKAVNTVAGKEHIIFENELMVVPPNVEHSLFTTGVVVNILAKTSYIKNN
                     FFTLFPYDNAFTNFFFNSVFSKDQYEYLTIDISSNCIRDLIYILISEGLNELSKNSTI
                     KNSLLQTIFLMIGKEYGNNIELPGDLAPTNSLVVEIVHYISSNYEHVTLEEVASKFNY
                     SVSYLSKIISSCMNMTFKEILYKIKIENALKLLSSTDESIEEISEKIGYINTASFYRA
                     FKNTMGMTPGEYRENCKKTQYIFKVNKKND"
     gene            complement(1713..3683)
                     /locus_tag="PLI11_04035"
     CDS             complement(1713..3683)
                     /locus_tag="PLI11_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ52065.1"
                     /translation="MKKYKLWVFPILIAFCVNTGCVKNNDKKVEKMPQYLKTTAIDKS
                     REGSLANYLVNMNISGQVGEIFEAHTEAWVTRVLEDNPGLFDGFLNPSNPDIRKAMWH
                     GEFPGKILSGIAQMYLTSKDRRIYEVGNRFVEYFKQAQREDGYLGPWPEEAKFNNDSR
                     SATEGPWGKWDTWGHYHCIHGLYRWYQVTGNEDALEIAEKALDCIIDHFITNKQSFAS
                     QKWGECNFAISHMFALMYDVTGKNKYLKAAEYMVNTEWKNEYLDFYTKRILSADWLNS
                     ALEGVVFGRSNQTRWESLYTLSTLGPLYKATGDETYLKALDSLWWGMVGYDRHNIGSF
                     GTGEGANANPYGDMSETCNTVAWAVFSTEYLKVSKNSYVADELEMSFFNAILGSLDKD
                     NRVFSYANYSNGQRDLATITLEGHSYAGARDMSCCQASGTKGLASITEWAILTDATGL
                     YLNYYGESKLETFTNNGNRIKLNQQTVYPKDGYIKIIVEPEQDEEFSLHLRIPVWSEH
                     SKIKLNGKPLENVNAGTYHAITRKWKKGDSIEIELDMSLHFWVAEKNSTMRNRSSIYY
                     GPLLLGVEENDDIRNTIKLDYGNLKNAKINTDKGFVYIKTLSTIGKEVTLVDFAHLGK
                     NNSSYFTWINVDNHNLQEITYDRNNTPVWCNR"
     gene            complement(3670..4974)
                     /locus_tag="PLI11_04040"
     CDS             complement(3670..4974)
                     /locus_tag="PLI11_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52066.1"
                     /translation="MRRIMNLILATIIFVSLVPFNTSAQDNPPVTTGTKATEIDENLI
                     KSKGCKIVDNESRDISYTWAYNLNWKADNDKAYGGNVLHNAGQVGSTISYDFAGNYIA
                     VAFCETYHAAKILIYIDDVLVAEHTPFANDKNLAVIPGESKIIYVNDSLENTNHTLLI
                     RHEVAHTSGEDNVKPDGNAYYDNDAYFDFIIVRDTEVKEEEPEEIVLGASIKGYTDEV
                     LGKLGYKAIYSNNEDIIYTWPFDLPQAKHMANNREYRFNLGQVSATLTCDFEGTYLVV
                     VFGECYYAAKVVVDVDGEIIGEFTPHTDKIKVSDTVYQSKIFLIKDDLKPGNHTVTIS
                     LETAYTTGEDNVKPDGKAYYDNDSFFDCFIVQKNPTPKEETPTPVSTETPQPTETVSY
                     TENPETSDKNSGIVAYVILSAIAILVLLAIIIFAKRRQHEKV"
BASE COUNT         1339 a         1046 c          973 g         1703 t
ORIGIN      
        1 aaatacgatg gtgaaaccaa aacggaattg tcactggatg gtgctgctac agatgcttca
       61 tcaacaaata atccatcctc caatgcgatt gatggcagca acaacaccaa gtggtcctcc
      121 gtaggcaaca gcgtaccaca gtggattcag gttattctgc cagaggttaa aaacctttcc
      181 ggattcagat tggttgctga tttcaactgg acggatatcc gtgcttacca tgttgaagtt
      241 gctacaaaag atggatgggt aaaggtctac gagcattctg gtgaaagcat tgagcacttc
      301 caggagattg acttcgatac attgtggaat atagattcaa ttcgtttcac cataactgaa
      361 gttggtccgg atgcacttca gggacatgga acaacttcca tagatatttt ggaaatcaaa
      421 ctattcacag cagatgctat cgaacaagaa acagagcctg aaacaaaccc acaaacaagc
      481 gacatgtcca taatcttcat ccttggaatg actgcagttt tgggcttgag cttgtttaag
      541 agaagaatga cagtgaacgc ataagttcat attgaacttt gaagaaccga agggaagcct
      601 tcggttcttt agtcattttt cttgtttact ttaaatatgt attgtgtctt tttacagttt
      661 tccctgtatt cgccaggtgt catacccatt gtatttttga atgccctata gaaacttgcg
      721 gtatttatat aacctatttt ttcggatatt tcttcaattg attcatcggt ggatgataaa
      781 agtttcaaag cattttcgat ctttatttta tacagtatct ctttgaatgt catattcatg
      841 caggaggata ttattttgct cagataggaa actgagtagt tgaatttact ggcaacctcc
      901 tcaagagtaa catgttcata gttgctgctt atgtaatgta caatttctac taccaggctg
      961 ttggtaggtg caagatcgcc aggaagttca atgttatttc catattcttt tcctatcatt
     1021 agaaatatgg tttgcagaag tgaattcttg atggtgctat ttttgcttaa ctcattcaaa
     1081 ccttcagaaa tcaatatata tatcagatcc cttatgcaat tactacttat gtctattgtc
     1141 aaatattcat actgatcttt tgaaaatacc gagttgaaaa agaaattggt aaaggcatta
     1201 tcgtaaggaa aaagtgtaaa aaagttgttc tttatatagc tcgtttttgc cagaatgttt
     1261 accacaacac cagttgtaaa aagagagtgt tccacattcg gagggaccac cattaattca
     1321 ttttcaaaga ttatgtgctc cttgcctgcc acggtgttta ccgctttgcc ttcatatacg
     1381 aaaagcattt cataaaaggt gtgtctgtgt ttgaaaggta tgtcatatct gccgtgtctg
     1441 tttatgtata tatcatcttc aaagaaatca taaatgattg tgggtcttgg catgtcgtcc
     1501 gtaatacccg ggagcgaaag aagaagatca tagcacctct ttttgtcaaa ggttctcatg
     1561 tagtccgggt cattctttcg ctccaggtac tctttgcata tatgttcctg ttcggtccag
     1621 ggcagtatga ttttatccaa atcatttttt agcatgtcta tcacattctt ttttaaatat
     1681 gatacaacaa tgtaatccaa catgcaagtt actcatctat tgcaccatac aggtgtattg
     1741 tttctgtcgt aggtaatttc ctgcaggttg tgattatcca cgttgatcca ggtaaagtag
     1801 ctgctattgt ttttgccaag atgggcaaaa tcaacaagtg tcacttcctt tccgatggtg
     1861 gaaagcgttt taatatatac aaagcctttg tctgtattta tttttgcgtt ctttagattt
     1921 ccgtaatcga gttttatggt atttcttata tcatcgtttt cttccacgcc aagaagcaat
     1981 ggaccataat atattgatga tctgttcctc attgtcgagt ttttctctgc aacccaaaaa
     2041 tgtagtgaca tgtcaagttc aatctctata ctgtcacctt ttttccactt tcttgttata
     2101 gcatggtagg tgcctgcatt tacattctcc aaaggtttgc cattaagctt tattttcgaa
     2161 tgttcagacc aaaccgggat tcttaagtgg agagagaatt cctcatcttg ttcaggctca
     2221 acaatgattt ttatatagcc atcctttgga taaacggtct gttgatttaa tttaattctg
     2281 ttgccgttat ttgtaaaggt ttcaagcttg ctttctccgt aatagttgag ataaagtcct
     2341 gttgcatctg tcaggatcgc ccattcggtt attgatgcca atccttttgt accgcttgcc
     2401 tggcagcagg acatgtctct ggcaccagca tacgagtggc cttcaagggt tattgtggca
     2461 aggtcgcgtt gcccattgga gtaattggca taggaaaaga ccctgttgtc tttatcaagg
     2521 cttcccaata tggcattgaa aaaggacatt tcaagttcat ctgccacata tgagtttttt
     2581 gatactttaa ggtactctgt ggagaataca gcccatgcaa cagtattgca tgtttcactc
     2641 atgtcaccat atgggtttgc attggcgcct tcaccggttc caaaactgcc tatattatgt
     2701 ctgtcatagc caaccatacc ccaccaaaga gaatccagtg ccttgagata agtctcgtca
     2761 cccgttgcct tgtatagagg accgagggtg ctgagagtat aaaggctttc ccatcttgtc
     2821 tggttggacc tgccaaagac aaccccttcc aaagcggaat tcagccaatc cgcacttagt
     2881 attctttttg tatagaaatc caggtattcg tttttccact cggtgtttac catgtattcc
     2941 gctgccttaa gatatttgtt cttgcctgtc acgtcataca tgagagcaaa catatgactg
     3001 attgcaaaat tgcattctcc ccatttctgg gatgcaaagg actgcttgtt ggttatgaag
     3061 tggtctatta tgcagtccaa tgctttttca gcaatttcaa gagcatcttc attaccggta
     3121 acctgatacc acctgtagag gccatggatg cagtggtaat gtccccaggt gtcccatttg
     3181 ccccagggtc cttcagttgc gcttcgcgag tcattgttga attttgcttc ttcaggccag
     3241 ggaccaagat atccatcctc tctctgagcc tgcttgaagt attcaacaaa cctgtttcca
     3301 acttcatata ttcttctgtc tttggaggtt agatacattt gtgcaatgcc tgaaagtatt
     3361 tttccaggga actctccatg ccacattgcc tttctaatat caggattgga tggattcagg
     3421 aaaccatcaa acagacccgg gttgtcttcc agaaccctag ttacccatgc ttcggtatga
     3481 gcttcgaata tctcacccac ctgtcctgaa atattcatgt taaccagata gtttgcaagg
     3541 cttccctccc tgcttttatc gattgctgtt gtctttaaat attgaggcat cttctcaacc
     3601 ttcttatcgt tattcttaac acagcccgtg ttgacacaga aggctatcaa tattgggaat
     3661 acccaaagct tatacttttt catgttgtct cctctttgca aagattatta tggcaagcaa
     3721 aaccagaatg gcaattgccg aaagtattac atatgcaact ataccgctgt tcttgtcact
     3781 tgtttccgga ttttccgtat aggatacagt ttccgtaggt tgtggagttt ccgtggagac
     3841 aggagtaggt gtttcttctt ttggagtcgg gtttttctgg actatgaagc agtcaaaaaa
     3901 cgaatcattg tcatagtatg ccttgccatc cggctttaca ttgtcttctc ctgttgtgta
     3961 ggcggtttcc aaagaaatcg tcactgtatg attccccggt ttcaaatcat ctttaataag
     4021 gaatattttg ctttgatata cggtatcaga cacttttatc ttgtcggtgt gaggtgtgaa
     4081 ttcccctata atctcaccgt ccacgtcaac aacaaccttg gctgcataat agcattctcc
     4141 aaagacaact acaagatatg tgccctcgaa atcacatgta agcgtggcac taacctgtcc
     4201 cagattgaat ctgtattcgc ggttgtttgc catatgtttt gcctgaggca aatcaaatgg
     4261 ccaagtgtat attatgtctt cgttgttact gtatattgcc ttgtagccaa gtttgcccaa
     4321 aacttcatca gtgtatcctt taatggatgc tcccaaaaca atttcttctg gttcctcttc
     4381 ttttacttcc gtgtctctta cgataataaa atcaaagtat gcatcattgt cataatatgc
     4441 gttgccatca ggctttacat tatcctcgcc ggaagtatgt gccacttcgt gcctgatcaa
     4501 aagggtatgg tttgtgtttt caagactgtc gtttacataa attatcttgc tttctcccgg
     4561 aataacagca aggtttttat catttgcaaa cggtgtatgc tctgcaacca atacatcatc
     4621 tatgtagatt aatatcttgg cggcatgata tgtttcacaa aatgccactg ctatgtaatt
     4681 gccagcaaaa tcataagata ttgtactgcc tacctgtcct gcattgtgaa ggacgttgcc
     4741 gccatatgcc ttgtcgttat ccgctttcca gttcaggttg tatgcccaag tatatgaaat
     4801 atcacggctt tcattgtcaa caattttgca cccttttgat ttaataagat tttcatcaat
     4861 ttccgttgct tttgtgcccg tcgtgacagg tggattgtcc tgtgcactcg tattaaaagg
     4921 gacaagtgat acgaaaatga ttgttgccag tatcaagttc attattcttc tcataactga
     4981 tccctccata ttatgcttac ctaaatttta taaatttaat aaagagtgat aatagcatga
     5041 cttggttcaa acacttgcat t
//
LOCUS       DAONVY010000107         5048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12037_length_5048_cov_3.472662, whole genome shotgun sequence.
ACCESSION   DAONVY010000107 DAONVY010000000
VERSION     DAONVY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12037_length_5048_cov_3.472662"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLI11_04045"
     CDS             <1..1341
                     /locus_tag="PLI11_04045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPZ52067.1"
                     /translation="MDNMYGVVEGLFTLVNLTLLTLAYGFAWFSEKLSIPFPFGGNFT
                     VLGVYAILLYTFTSLYGGYQIGQLKRLDVINSQIISILFVNAITYLQISLMERTLVIS
                     KALPIVILTLVDIVIIIVWAYIADAVYKKMYPPRDIALIYDNPFDLIEVEDKLNMRKD
                     KFNLKVVLSSDEDFEAVKKALDECGVVALASEKEPLRENILKYCFEKGISVYISPKLS
                     DLMIRGASRLELFDSPIYYCSNKGLTIGQRIIKRLMDMILSLVLIILFSPVMIVIAAA
                     IKLHDKGPVLFKQKRYTKDGKVFNILKFRSMIVDAEKDGEVIPAEDNDPRITPVGKVI
                     RKIRMDELPQLFNILKGDMSVVGPRPERVEHVDMYSEQIPEFRYRLKVKGGLTGYAQV
                     LGKYNTSPYDKLRLDLIYIENYSLLLDLKLMLTTIKILFKPESTEGFNKGDEKR"
     gene            1342..2565
                     /locus_tag="PLI11_04050"
     CDS             1342..2565
                     /locus_tag="PLI11_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPZ52068.1"
                     /translation="MDIYVVSEYYYPDNFKVNDMVREFVQRGHNVTTLAGLPDYETGT
                     VRKEYRFFRRRKETVDGAKVVRVSTVSRRTGAFFRILTYSSFAFNGFIHTKLCRKPSC
                     DVIFVNQITPILQAIPALSLGKRIKKPVVLYCMDIWPEVLKAWDIKESSLFFRCAMAL
                     SKKLYRKCDLILISSKPFREYLTKVIGVDDERIVYLPQYAEDLYENIAGKYLENGVVD
                     FLFAGNIGSVQDIECIIKAVKHIEDNNLNNGKEYKVHIVGDGINLENCKNLTKELDVV
                     NQVVFHGRHPLSEMKRFYEMADCFLLTMRGGDFISMTLPGKAQSYLSVGKPIAGAIDG
                     AAMEVINEADCGLCVRGSDHEGLASIMAQIMLDFETYKEKGHNGRKYYLDNFTKDSFF
                     DSLFDIFVDLQESLR"
     gene            2603..3718
                     /locus_tag="PLI11_04055"
     CDS             2603..3718
                     /locus_tag="PLI11_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019377585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HPZ52069.1"
                     /translation="MKKILVTGAKGFVGKNLCLSLKNSEKYEVMEYDVDTEESLLEEF
                     IMQADFLVHLAGVNRPKEDKEFETGNHGFTSQIIDMLEKRDKRTSIIFSSSIHAEKDN
                     PYGTSKRNAEQVIFDYGRRNNAEVFVYRLPNLFGKCCRPNYNSVIATFCHNIARDLPI
                     TVNDPNYVMNVAYIDDVVDEFINAIEGRANIKEDGFCYVPITYTRALGEIAILIQSFK
                     ESRKNLLVPNMDDELCKKLYSTYLSYLKEDDFSYPLKMNVDHRGAFTEFLKTPERGQV
                     SVNVSKPGIVKGNHWHHTKNEKFLVVSGEAVIRFRKIGEKDIIEVRVSDKELRVVDIP
                     PGYTHSIENVGQTDLVTIMWANECFDPERPDTYYEEV"
     gene            3729..4853
                     /gene="wecB"
                     /locus_tag="PLI11_04060"
     CDS             3729..4853
                     /gene="wecB"
                     /locus_tag="PLI11_04060"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPZ52070.1"
                     /translation="MEKLKVMTIIGTRPEIIRLSECIKACDKYFNHILVHTGQNWDYT
                     LNQVFFEDLGLREPDYYLNAAGSNLGETIGNIVAKAYEVLEKERPEALLILGDTNSAL
                     SAIPAKRLKIPIFHMEAGNRCFDQNVPEEINRKIVDHISDINLPYTEHSRRYLLSEGI
                     RKEHIFVTGSPMKEVIVKNMEKIEKSNVLEELSLEKNRYFVISAHREENIDNEEHFFS
                     LMNAINEVAETYNMPVVYSIHPRSQKMIEKREFKFHPLVKQLRPFGFFDYNKLQKESF
                     CVLSDSGTLSEESSMLKFPAVLIRTSTERPEVVDKGSIVIGGITERDVLQSIELVRGI
                     WENGDVFSEPLDYEDDNVSIKVVKIIQGYSKIINEFVWRK"
BASE COUNT         1617 a          810 c         1207 g         1414 t
ORIGIN      
        1 atggacaaca tgtacggtgt tgtggagggt ttgtttacat tagtaaacct gacccttctt
       61 acccttgcat acggttttgc atggttttca gaaaagctgt ccataccatt tccttttggt
      121 ggaaacttta ccgtactagg cgtgtatgca atacttctgt atacctttac aagcttgtac
      181 ggtggctatc aaatagggca gctcaaaaga cttgatgtga ttaattcaca gataatctcc
      241 attttgtttg taaatgctat tacgtatctg caaatcagct tgatggaaag gactcttgta
      301 atttcaaaag cactgcccat tgttattctt actctggtgg atatagttat aattattgta
      361 tgggcgtaca ttgcggatgc agtgtacaag aaaatgtatc ccccaaggga cattgctcta
      421 atatacgaca acccttttga cctgatagaa gttgaggaca aactcaatat gaggaaggac
      481 aagttcaact taaaagtggt attaagctcc gatgaagatt ttgaggcagt taagaaggcc
      541 ttggatgaat gcggtgtggt tgctcttgcc agtgaaaaag aacctttgag ggagaacata
      601 cttaagtact gctttgaaaa agggataagc gtatatatat ctccaaagtt gtcagatttg
      661 atgataagag gagcgtcacg attggagctt ttcgattctc ccatatatta ctgcagcaac
      721 aaaggcctga caatagggca gaggataatt aaaagactga tggacatgat tttatccctt
      781 gtactgatca tactgttttc acctgtcatg atagttattg ctgctgcgat aaaattgcat
      841 gacaagggcc ctgttctttt caaacaaaaa agatatacca aagatggaaa ggtgttcaac
      901 atactgaagt tcaggagtat gattgtggat gcagaaaaag atggagaggt aataccggcc
      961 gaagacaacg accccaggat tacaccagtt ggaaaggtta taagaaagat tcgcatggac
     1021 gagcttccgc agcttttcaa tatcctgaaa ggagacatga gtgttgtggg gccaaggccg
     1081 gagagggttg aacatgtgga tatgtacagc gagcagattc cagagttcag atacagactg
     1141 aaggtaaagg gcggacttac aggatatgcc caggttcttg gcaagtacaa tacaagccca
     1201 tatgacaaac tgagacttga cctgatttac attgagaact attcgcttct tctggacctg
     1261 aagcttatgc tgactacgat aaagatacta tttaaacccg agagtactga aggattcaac
     1321 aaaggagatg agaaacggta gatggacatt tatgtggttt ctgaatacta ttacccggac
     1381 aacttcaagg ttaacgatat ggtaagggag ttcgttcaaa gaggtcacaa tgtaaccaca
     1441 ctggcaggcc ttcctgatta cgagaccggt actgtaagga aagagtacag gttttttaga
     1501 aggagaaaag aaacagttga tggagcaaag gttgtcagag tatctaccgt ttcaagaaga
     1561 acaggcgctt ttttcagaat tcttacgtac tcgtcttttg cattcaatgg ttttattcat
     1621 acaaagcttt gcaggaaacc atcctgtgat gtaatattcg taaatcagat tacccccata
     1681 ctgcaggcca tacctgccct gtcgctggga aagagaataa aaaaacctgt ggttctttac
     1741 tgcatggata tatggcccga agttttgaaa gcctgggata ttaaagagag ttctcttttt
     1801 ttcagatgtg ccatggcttt gagtaagaaa ctgtacagaa aatgtgatct gatacttata
     1861 tcatcaaagc cttttaggga gtatctgacc aaagtaatag gagtggatga tgaaaggatt
     1921 gtttatctgc ctcagtatgc ggaggatttg tacgaaaata ttgcaggaaa atatcttgaa
     1981 aacggagttg ttgatttttt atttgcaggc aatataggtt ccgtacagga tattgagtgc
     2041 attattaaag cggtaaaaca tattgaagat aataatttga acaatggaaa ggaatacaag
     2101 gtccatattg ttggggatgg gataaatcta gagaactgca aaaatctcac aaaagaactt
     2161 gatgttgtta atcaagtggt tttccatgga agacaccctc tttctgaaat gaagaggttt
     2221 tacgaaatgg cggattgctt tctcctgaca atgcgtggcg gagatttcat aagcatgaca
     2281 ctgcctggca aggcccagag ttatttaagt gtgggcaaac ccattgcagg agcaatagat
     2341 ggggctgcca tggaagttat aaatgaggca gactgtggtt tgtgtgtaag aggaagcgat
     2401 catgaaggac tggcttccat catggctcaa atcatgttgg attttgaaac atacaaggaa
     2461 aaaggtcata acggaagaaa atattatctt gataatttta caaaagacag tttttttgac
     2521 agtctttttg acatatttgt tgacttgcag gaatcgctgc gttaatatat ttaagtagtg
     2581 ttttggaaaa ggtggcggtt ttttgaagaa gattctggtt actggagcaa aaggtttcgt
     2641 tggaaagaat ttgtgtcttt ctctgaaaaa cagtgaaaag tatgaagtta tggaatatga
     2701 tgtggatacg gaagaaagct tgcttgaaga atttatcatg caggctgact ttctcgttca
     2761 tttggcaggc gtgaacagac ccaaggagga taaggagttt gaaacaggca atcacggctt
     2821 tacaagccag ataattgata tgcttgagaa gcgtgataaa aggacaagca taatcttctc
     2881 gtcatccatt cacgctgaaa aggacaatcc ttatggaaca agcaagagaa atgcggaaca
     2941 ggtcattttt gattatggca gaagaaacaa cgcagaagtt tttgtgtaca ggcttccaaa
     3001 cttgtttgga aagtgctgca gacccaacta caacagcgtt attgcaacct tctgtcataa
     3061 tattgcgagg gatttgccca taactgtaaa tgatcctaat tatgtcatga atgttgccta
     3121 cattgacgat gtggtggatg aattcataaa tgccattgag ggaagggcaa atatcaagga
     3181 agacggattt tgttatgtac ccataacata tacaagagcg ttgggtgaaa ttgcaatact
     3241 tatacaatcc ttcaaggagt cgagaaagaa tctgcttgtt cccaacatgg acgatgaatt
     3301 gtgcaagaaa ctctacagca catatcttag ctatctcaag gaagatgatt ttagttatcc
     3361 tcttaagatg aatgtggatc acagaggagc ttttaccgag ttccttaaaa cgcctgaaag
     3421 aggacaggtg tcggtaaacg tatcaaaacc aggcattgta aagggaaatc actggcatca
     3481 caccaagaat gagaaatttc tggttgtcag cggagaggca gtaatcaggt tcagaaaaat
     3541 tggggaaaag gatattatcg aggtgcgtgt gtcggacaag gagctaagag ttgtagacat
     3601 accaccagga tacacccatt caattgagaa cgtgggacag acggatctgg taactataat
     3661 gtgggcgaat gagtgttttg acccggaaag accggataca tattatgaag aggtgtagga
     3721 gagagaatat ggaaaagctg aaggtaatga ctattattgg aacaagaccg gagattatta
     3781 ggttgtcgga atgtataaag gcttgtgaca aatatttcaa tcacatactt gtacacacgg
     3841 gccagaactg ggattatact ttgaaccagg tgttttttga agatcttgga ttaagagagc
     3901 ctgactatta tctaaatgct gcaggcagta atctgggtga aaccataggc aatattgtag
     3961 caaaggcgta tgaagttctt gaaaaggaaa ggcctgaggc gctattgata cttggagaca
     4021 ccaattctgc tttaagtgca attccggcaa agaggctcaa gatacccatt ttccacatgg
     4081 aggcgggaaa tcgctgtttc gaccagaatg ttcctgagga aataaacagg aagattgtag
     4141 accatataag tgatattaat cttccgtata ctgagcacag cagaaggtat cttttgagtg
     4201 agggcataag aaaagagcat atttttgtaa ccggctcgcc catgaaagaa gtaattgtca
     4261 agaatatgga gaagattgaa aaaagcaatg ttcttgagga gttatctttg gagaaaaata
     4321 gatatttcgt gatatctgcc cacagggaag agaatataga taatgaggaa cactttttct
     4381 cgctgatgaa tgcaataaac gaggtggcgg aaacttataa catgcctgtt gtttattcca
     4441 ttcacccaag aagtcagaag atgatcgaga aaagggaatt caagttccat ccattggtca
     4501 agcagctaag accttttgga ttctttgatt acaacaagct tcagaaggag tctttctgtg
     4561 tcctttccga cagtggaaca ctgtcggaag aatcttcgat gcttaagttc cctgcagtac
     4621 ttataaggac ttcaactgaa agacctgagg ttgtcgacaa gggaagcatt gtaattggtg
     4681 gaataactga aagggatgtc cttcagtcca ttgaacttgt aagggggatt tgggaaaatg
     4741 gagatgtttt ctcagagccg ttggattacg aagatgacaa tgtttccatc aaggtagtaa
     4801 agataataca gggttattca aagataataa atgagtttgt ttggagaaaa tagggaaagg
     4861 gcggtgattt ttgagggtct tcactttgaa ggcgagatga tatgcgcact gtttttagat
     4921 atttaaaacc atattatttc agaatgtcga ttgggtttct aatcaaattt actggaacca
     4981 taatggattt gttactgcca tggatattgg catatataat agataatgta atagctaaag
     5041 agaatctt
//
LOCUS       DAONVY010000108         5040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12068_length_5040_cov_3.197793, whole genome shotgun sequence.
ACCESSION   DAONVY010000108 DAONVY010000000
VERSION     DAONVY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12068_length_5040_cov_3.197793"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2057)
                     /gene="nifJ"
                     /locus_tag="PLI11_04065"
                     /pseudo
     CDS             complement(1..2057)
                     /gene="nifJ"
                     /locus_tag="PLI11_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634471.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            complement(2256..3173)
                     /locus_tag="PLI11_04070"
     CDS             complement(2256..3173)
                     /locus_tag="PLI11_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPZ52071.1"
                     /translation="MKKVIFIVITLFLAASSLSVLAEETENEDGLYLTKIEYVSATGY
                     VTPIFDIEGTIRHDNEDFKYPHSIEAHPISVSEPSEIVYDISEYNYTHFAAYLGKNAV
                     LEARGAHVVFVVWADGEKIYESETVAVGDLPVACVAKIPEGTKELKLEVNAGIDGVDY
                     DTSTWGSPMLVNLEVTDFKEKRQVSKTEYKVGEEFSPNGGFYTIVYSNGMEVDGMLTE
                     DMISGFDSETPGAKTITVTYEEKTYTFEVTVLEDETPAPTQSQKETKEPTDESEKDGG
                     NLGIIIAVVVATAVVVAVVVAIIVLRKKQ"
     gene            complement(3191..4234)
                     /locus_tag="PLI11_04075"
     CDS             complement(3191..4234)
                     /locus_tag="PLI11_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52072.1"
                     /translation="MKRRVVFILIVLILVLTTACNRNGGGNVKYIEREPADCDIGIWY
                     SVWYAYLDEMSNSVQRNTWNAWDIQYDVLLPDGTYGKYDSLDEDIIFFHLEQMADLGI
                     DFVIMDQTNHIDVENGYINERALKFAQCIKEWNDIPENRPIKYCSAIGAIQWSNNAAS
                     IEQEAKILWDRYCMQEFGTSDYHYYLDDKPLLIVYGDGGEELWNNYDGKKTYGEMFTI
                     RYADNGSNPGRYGWAFDKGTQLHEEVVVVMPGWNNRKGAPPVMRNQGKWYREQWEKLL
                     MAEKKPEIVVINSFNEYAENTAIFPADTSKMTNEIEKWIDENGEISTTMYWKMTKDYI
                     EQLRSGKYKVFEN"
     gene            4610..>5040
                     /locus_tag="PLI11_04080"
     CDS             4610..>5040
                     /locus_tag="PLI11_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPZ52073.1"
                     /translation="MEHANPLGEERIVKLLARFSIPAIVGMMVNALYNIVDRIFIGNS
                     DYLGTNGLAAATVCFPIMIIVLAVGLLFGIGGATLFSIKLGEGKKEEAERVLGNAFVL
                     LVLGGIVVSILGFAFIRKLLIYFGASDDTLPYTEECISVIF"
BASE COUNT         1348 a         1195 c          911 g         1586 t
ORIGIN      
        1 tacggcgacg ccgcgtttct cggatgcggc agaaccctgc gggaatgtac cgtctgcatg
       61 atccataaac acggatacag gcaatgaatc tccatccatc agtgcagcag gcttgaggat
      121 gttcttaacg aacttaacaa gctctggacg atctcctgta atctgttctt ctgcttgtgt
      181 tgtatcaaga ttcttccatg attcaggtac gtcaacctta actattgcat taacacctgc
      241 gtcaatggct ttccagttca tttcaactat cgcttcgccc ttggagatgt aggaatctct
      301 tgcagctttc ttcatgtact caacagcctt gtcaattggc aggatattcg caattgcaaa
      361 gaatgcagac tgcagaactg tattggtacg cttgcccatt cctatctcac gagcaatatc
      421 tattgcattg attatataaa agttaatgtt attgtcagca atatatttct taaccttgtt
      481 tggcagatgt gtatccagct cttcaggagt ccactggcag ttaagcagga aggatcctcc
      541 tggcttgaca tcacgaacaa tttcaaatcc ttttactatg tatgatgggt tgtggcaggc
      601 aacaaagtcc gccttcttta catagtatga tgacttgata ggctggtcac caaatctcag
      661 gtgggaaatg gttactccgc ctgtcttctt tgagtcgtat tggaagtaag cctggatgta
      721 tttatctgtg tggtcaccaa taatcttgat ggagttcttg ttggcaccaa cagtaccgtc
      781 accgccaaga ccccagaact tgcagctgat ggttcctttt ggctgggtgt caggtgaagg
      841 cttctcttca agagacagat gtgtaacgtc gtcaactatt ccgattgtaa agccgttctt
      901 tggctcatcc ttttcaagat tctcataaac tgcaaatatg cttgatggtg gtgtatcctt
      961 tgaacccagt ccgtaacgac cacctacaac cttgatatta ataccgtttg tagcaagagc
     1021 ggtaacaaca tcaagataca aaggttcacc cagtgcacca ggctctttag ttctatcaag
     1081 aaccgctatc ttcttcactg tctttggcaa tgcttcgata agcctgtccg ctctgaaagg
     1141 acgatacaat ctaaccttga tcaatcctac ctttcgccct tatcaatcag gtaatctata
     1201 acttcttcaa ttacgtcgtt taccgaaccc atagcaatga taactctctc agcctcaggg
     1261 tgtccgtagt agttgaacaa tttatagttt gtgccaatct tcttgttaac catttccata
     1321 tattcttcaa cgatggcagg aagagcgtca taatacttgt tacatgcttc cctgtgctgg
     1381 aagaatatat ctccgttttc tgcacttccg cgtagtactg ggttttcagg attcaaagct
     1441 ttgtttctga attcagcaag agcttccttg tctatcattt catccagatc attgtagtcc
     1501 caaactgcga ccttctggat ttcatggctt gtcctgaatc cgtcaaagaa attaatgaat
     1561 ggtatgcgac ccttaattgc agctagatgt gcaattgctc caaggtccat gacctcctgt
     1621 gcatttgtgc agcatagcat tgcgcaacct gtctgacggc atgcatagac gtccgaatgg
     1681 tcgccaaaaa tattcagtgc atgcgatgca acagttcttg ctgaaacatg aaccactcct
     1741 ggcaataact ctcctgcaat tttatacaag ttgggaatca ttagaagtaa accttgagaa
     1801 gcagtatatg tagtagttaa tgcgcctgaa gcaagacttc cgtgtactgc tccagctgct
     1861 cccgcctccg attgcatttc tacgaccttt acattgtcgc caaaaatatt cttgagtcca
     1921 ttcgcagacc aagagtcggt aagctcggcc ataacactgg aaggtgttat tggataaatt
     1981 gctgctactt cagtaaaagc ataactgaca tgcgccgcag cggtgttacc gtccattgat
     2041 ttaaactttc ttgtcatgta aaatgcctcc tatattaatg agctatattt gaaaaacggc
     2101 gattgtgttt tatcaaacaa caacgttaat atcttaacat attttaatgg taaagtaaag
     2161 aaattatatg ttaacaacta ttcaacccaa tgccttccat caataaaaat ttcacctctc
     2221 atacataacg agaggtgaaa ctggattatt gttttttatt gcttttttct aagtacaatt
     2281 atcgccacaa ctactgccac aaccaccgct gtagccacca caacagcaat tataatacca
     2341 agattaccgc cgtctttttc agactcgtct gtgggttcct tcgtttcctt ctgcgattgt
     2401 gttggtgctg gtgtttcatc ttccagtacc gtgacctcga aggtataggt cttttcttca
     2461 tatgtaacgg ttattgtttt ggcacctggg gtttcactat cgaacccaga aatcatgtct
     2521 tctgtaagca taccgtctac ttccattccg ttcgagtaga caatggtata gaatcctcca
     2581 tttggcgaga actcttctcc aaccttgtac tctgttttac ttacctgtct cttctccttg
     2641 aaatcggtta cctcaaggtt cacaagcatt ggagagcccc aagttgatgt gtcatagtcc
     2701 acaccgtcaa ttcctgcgtt aacctccagt ttcagttcct tcgtcccctc aggaattttg
     2761 gcaacacatg caacaggcaa gtctcccact gcaactgtct cactttcata aatcttctca
     2821 ccgtccgccc aaaccacaaa gacaacatgt gcgcctcgtg cttccaacac tgcgtttttg
     2881 cccaaatatg cagcaaaatg tgtgtagttg tattcagaaa tatcgtatac aatttcactg
     2941 ggttctgaaa cgcttattgg atgggcctca atggaatggg gatacttgaa atcttcatta
     3001 tcatgcctga tagtaccctc aatgtcaaag atgggagtta cgtaccctgt tgcacttacg
     3061 tactcaatct tggttaaata caagccatct tcgttttcag tctcctcagc gaggacggaa
     3121 agcgatgatg ctgcaagaaa taatgtgata acaataaata tcactttttt cataacgcaa
     3181 gcctccttta tcaattctca aaaactttgt attttccact ccttagctgc tctatgtagt
     3241 cctttgtcat cttccagtac atggttgtac taatctcgcc attttcatct atccacttct
     3301 cgatctcatt ggtcatttta cttgtgtccg ccggaaaaat cgcagtattc tccgcatact
     3361 cgttgaaaga atttataact acgatttcgg gcttcttttc agccatcaac aacttctccc
     3421 actgttccct ataccatttg ccctgattgc gcattaccgg tggtgcacct tttctattat
     3481 tccacccagg catgaccaca accacttcct catgcagctg tgtgcccttg tcaaacgccc
     3541 acccgtaacg tccaggatta ctaccattgt ctgcatatct gatggtgaac atctctccat
     3601 atgtcttctt cccatcgtag ttgttccaca actcttctcc cccatctcca taaacaataa
     3661 gcaggggttt gtcatccaga tagtagtgat agtcggatgt tccaaactcc tgcatgcagt
     3721 atctatccca aagtattttt gcctcttgtt caatacttgc agcattgtta gaccattgta
     3781 ttgcgccaat agccgagcag tatttgatag gcctgttctc aggaatgtca ttccattcct
     3841 ttatgcattg tgcaaatttc aatgctcttt cgttaatata gccattttcc acatcaatgt
     3901 gattcgtctg gtccataatc acaaaatcta tgccgagatc agccatttgc tccaagtgaa
     3961 agaatataat gtcctcatcc agcgaatcat attttccgta agttccatca ggaagcaaaa
     4021 catcatactg tatgtcccat gcattccaag tgttcctttg tacagagttg gacatttcgt
     4081 caaggtacgc ataccataca ctgtaccaga taccgatatc acaatcagca ggctctcttt
     4141 caatatattt cacattgcca cctccatttc tgttgcatgc tgttgtcaga accaggatta
     4201 gaacaatcaa tatgaaaacc actcttcttt tcatcatgct ccctccattt tacattcatt
     4261 aaagcgaaaa aaacaccaca actcgtcatg gcattaaata cgtccacaat cccccaacga
     4321 ttttacatat gcaattaaca cacataacac acaaaagccc gtaccttgtc atattggcac
     4381 accgcctttt tcggcctcaa tttatatact atatataata tacactaaat taatactcat
     4441 gtaaagacac cccactaata taagtgctcc caaaccaaac ttccgggtcc tgggtgctcc
     4501 tttaaagatt tgtttataaa acctttcaat acatcggtgg acatgagcta tactattatg
     4561 taatatatat gcactttcaa aaaaataaat aatattatag gagtagtata tggaacatgc
     4621 aaatccttta ggagaagaaa gaattgttaa attgttggcc agattttcaa ttcccgcaat
     4681 agtgggaatg atggtcaatg cactttataa cattgtcgat agaatcttca taggcaacag
     4741 cgattatctt ggcaccaatg gtcttgcagc cgccacggtc tgttttccca taatgataat
     4801 agttttggca gtgggtcttt tatttggcat tggaggagcc actctgtttt ctattaaact
     4861 gggagaagga aaaaaggagg aagctgagag ggttcttggc aatgcctttg ttcttttggt
     4921 tctcggcggt attgttgtat caatattggg ttttgctttc attagaaaac tcctgatata
     4981 tttcggtgcc agcgatgaca ctcttcccta tactgaagaa tgcattagtg tcattttctt
//
LOCUS       DAONVY010000109         5017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12134_length_5017_cov_3.835953, whole genome shotgun sequence.
ACCESSION   DAONVY010000109 DAONVY010000000
VERSION     DAONVY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12134_length_5017_cov_3.835953"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLI11_04085"
     CDS             complement(<1..270)
                     /locus_tag="PLI11_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endoglucanase"
                     /protein_id="HPZ52074.1"
                     /translation="MKKTILITLLLFLLFTSSCGTEDNIGTKTQEPVIEHTDEPSTPT
                     PVSKTPTPTVSKETEVPKTKTPEDTPNATPEPTKTPQETTPKPTST"
     gene            complement(940..1623)
                     /locus_tag="PLI11_04090"
     CDS             complement(940..1623)
                     /locus_tag="PLI11_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52075.1"
                     /translation="MGKRIVGTIAILVVLIVLAACNGSSEYLLTKEVNLDADGNVEFY
                     ITYEYENDEQNIVKKTKYNKDDEIEYYSVIMYDEQGREVESTSFNKEGQIENVCNWIY
                     DEDGYTIVEYKEGHEAKEVVDTSFNENGDVAVKEFLDTRIVEEYEYDENGFLVMVKST
                     LGEVTTYTKYINDEKGKVLKAEYFVTEDDVLIYTRYYYDMKGNLVGEKEFYSNGNLIS
                     EKEYEYTKK"
     gene            complement(1636..2403)
                     /locus_tag="PLI11_04095"
     CDS             complement(1636..2403)
                     /locus_tag="PLI11_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52076.1"
                     /translation="MLRRLPILFILILLLLALSACGGKAKYLMTKQTVCDADGGVRYY
                     ILYEYDKKNNLVKETDYDKDGKIRYYTTYQYDRDGRLVISEKYIGSKLESRAVYKYDD
                     EGREIERKLYNDSGVAYSTKIYTYEESGYTEVTYGENSNLQSTRKVTMNEKGDILRVE
                     HVTSLNLKITEEYAYDENGYLIKCDVKSEDAYNVYTLYINDSKGKVLVREDYQDGKLA
                     RYLEHTYDKNGNLIKIEVFSPQDTLTSSTRYVYKRAN"
     gene            2575..3696
                     /locus_tag="PLI11_04100"
     CDS             2575..3696
                     /locus_tag="PLI11_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ52077.1"
                     /translation="MKLDLDIEQFWKDDELAHKENCFSKEAPQVALGIRMSDECVFAE
                     LGEEGQPWGHTPLERRIELNKRYNDKALKIVGRKLLREEFPDPDSAFPPVKGIGEVFG
                     GKYVFDGNTTWLKRTINTPEELKERLDIIDKMDIRSFILPPNWESEKKRIYETYGKKP
                     GLFRGVRGPVTLATSVFGAENLIFLIYDDPDLAKRFSDTITKVLLAYIDIFIEEAGYS
                     QDNFPHGFGFNDDDCCLLTPEMYEFFAYPILKTVFDKTAPNPEDWRYQHSDSDMGHLL
                     PILARFNLTGCNFGPTVTVREIRKHMPNTRIDGQLSPLTFMRNDEEQITKEVLRDIEM
                     AKENDARGLNLTTAGSINNGSLLSSMRLIMALIQNYGRY"
     gene            3715..4248
                     /locus_tag="PLI11_04105"
     CDS             3715..4248
                     /locus_tag="PLI11_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HPZ52078.1"
                     /translation="MEIRVSTVVNYDEYKTFFRFSMFRGKGYRFKSFSSVLIMLCAIA
                     VSLTGTIFYYNPLMLFAFIFSLLMTVTLLIIWFYMPRYSYINTFITKGSKDDYAFLDD
                     RILVNTVTEHIKSQTEISYSQIYKIYETKKHIYIYLNPGFAYIISKKAFVNHADIAEL
                     RSFFVAKMPEKYIHCTF"
     gene            complement(4253..4663)
                     /locus_tag="PLI11_04110"
     CDS             complement(4253..4663)
                     /locus_tag="PLI11_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HPZ52079.1"
                     /translation="MLVDILVKDLLPAVRSLLKVCEGVRFTAVTSGMEPLVRKGIDKI
                     EIVYSHGRDLEIGDIVMLEPAEGIFVLRRIYRKTDLGYVTKGDAYKRTDGIVFPEHVV
                     GVVSKIYRREEVMHAEKIRQRFKIPLWRKIVPLR"
     gene            complement(4665..>5017)
                     /locus_tag="PLI11_04115"
     CDS             complement(4665..>5017)
                     /locus_tag="PLI11_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52080.1"
                     /translation="FGTTKTLLGTFCKAKLGDIVLFRMCSKYILHRIYKIRGENVYTI
                     GDACLFSDGSVPVCDIVGRVERIFRGDLDIDCNSVGWCLVFAMWRKLRIFRRQLLYLY
                     SLMARIRRKIIKGA"
BASE COUNT         1417 a         1058 c          905 g         1637 t
ORIGIN      
        1 agttgaggta ggctttgggg ttgtttcctg tggtgtcttt gttggctcgg gtgtggcatt
       61 tggcgtatct tcaggggttt tagttttagg aacttctgtc tccttgctta cagtaggtgt
      121 aggtgtttta ctcacggggg ttggagtgct aggttcatcc gtatgctcaa taactggttc
      181 ttgagttttg gtacctatat tgtcttcagt accacaggaa gaggtaaaga gcaggaataa
      241 taaaagtgtt attagaattg tctttttcat tcatctttcc cctttatcaa ggattcgtgt
      301 ttgatgggta taaagtaaca taacaggaag ctgattgcaa tatgaaactg gggcggatat
      361 tgtcgattta gcaatatgat ttcccaacaa caatatttcc tgacatatcg ggctgacttt
      421 tatgattttt tgtcttatat atatggtctg ctctgctaga gaaaacaatt attctgtctg
      481 ctatatggga tgcactaaag tggcgtgaaa taaagatggc ggtttatcca gttttcgtcc
      541 tttgaaagtg agtaagattg agattgctgt cagagaagtt attacgatta aaaaaaaggt
      601 atgtaagtac aaataccgga tttgctccca tggatttctt tttcatgtca agtaccgtgc
      661 agtcctgagc ccaatgtatt gacaatgatg ccatctttca tgtattcaag ttgaatcttg
      721 tcggaagctt ttcgcatttt gacattgcga agatattcgc cataccagat tcctgttccc
      781 tttcatcaaa gattcaatta ttacgatgcc tatgacattt acaaaagatt gtacagcgga
      841 caatatgttt tctataacaa ctacaaacaa aaatgatttt taatcttttt tagagtatct
      901 gacaagttcc ttgaaagctt caggtttttt catagtcact tattttttcg tgtactcata
      961 ctctttttcg cttataagat taccgtttga gtaaaattcc ttttcaccca ccaaatttcc
     1021 cttcatgtcg taataatagc gggtatagat caacacgtcg tcttccgtaa caaagtactc
     1081 agcttttaac actttccctt tttcatcgtt gatgtacttt gtgtatgtgg tgacttcacc
     1141 caaagtgctt ttaaccatga caagaaatcc attctcgtca tactcgtatt cttctacaat
     1201 gcgggtgtcc aaaaattcct ttacggccac atcaccgttc tcattaaaag atgtatccac
     1261 cacttctttt gcttcatgcc cttccttgta ttcgacaatc gtgtagccat cctcatcata
     1321 gatccagttg caaacatttt caatttggcc ctctttgttg aagctggttg actcaacttc
     1381 tcttccctgc tcgtcataca tgataacact gtagtattca atctcatcgt ctttattgta
     1441 cttggttttc ttgacaatgt tctgctcgtc attctcatac tcataggtga tatagaattc
     1501 cacattgcca tcagcgtcca aattcacttc cttcgtcagt aaatattccg agctgccgtt
     1561 acaagctgcc aatacaatca gcaccaccag tatagcaatt gttccaacaa ttctctttcc
     1621 cattttttcc tcccatcaat ttgccctttt atacacatat cttgtactgc ttgtcagagt
     1681 gtcctgtggt gaaaacacct cgattttaat gagattccca tttttatcgt aggtgtgctc
     1741 caggtatctg gccagtttcc catcctgata atcctcacgc acaagtactt ttccttttga
     1801 atcgtttata tataaagtat atacgttata tgcatcttca ctttttacat cgcatttaat
     1861 gagataacca ttttcatcat acgcgtattc ctcagtgatt tttaaattca aacttgttac
     1921 atgttcaact cttaatatat ctcctttttc attcattgtc acttttcgtg ttgactgcag
     1981 gttggaattc tcgccatatg tcacttccgt atatcctgat tcctcatagg tgtatatctt
     2041 tgttgagtat gcaactccac tgtcgttgta aagtttcctt tcaatctccc tgccctcatc
     2101 atcgtatttg tatactgcac ggctttccaa tttgctgccg atatattttt ctgaaattac
     2161 aagcctgcca tccctgtcat actgatatgt tgtatagtat ctaatcttgc catccttgtc
     2221 atagtccgtt tccttgacca gattgttctt tttgtcatac tcatatagta tgtaatacct
     2281 cacgccgcca tccgcatcac agacagtctg ctttgtcatc aaatattttg ctttgccacc
     2341 gcaggctgac aaagcaagta atagtaatat cagtatgaac aaaatgggta atcttcttaa
     2401 catgccttcc tccaactgtg tttatacgtt gattatactg cacagaccaa tttgggacaa
     2461 ctgcacttta tccctatttt attaacacat cgtgaacaaa tctcgctttt cgtgttttat
     2521 caagggttgt aaactcaccc atactgtgct aaaatcagcg cacgaggtga tttgatgaaa
     2581 cttgatttgg atattgaaca attttggaaa gatgacgaat tggcccacaa agagaactgt
     2641 ttctcaaaag aggcgccaca ggtggcactg ggtataagga tgagcgatga atgtgtcttt
     2701 gcagagctgg gagaggaagg tcagccttgg ggacatactc ctcttgaaag aagaattgag
     2761 cttaacaaaa gatacaacga taaggctttg aaaatagtgg gaagaaaact gcttagggaa
     2821 gagttccctg atccggattc cgcttttcct cctgtcaagg gtatcggaga agtgtttgga
     2881 ggcaagtatg tatttgatgg aaacactaca tggctcaaaa gaaccataaa cactccggaa
     2941 gagctgaagg aaagacttga tattatagac aagatggata ttagaagttt catacttcca
     3001 ccgaactggg aaagtgaaaa gaagagaatc tatgaaacct acggaaaaaa accgggtctt
     3061 ttccgtggtg taagaggtcc tgttacattg gccacatcgg ttttcggagc agaaaacctg
     3121 atattcctca tttacgacga cccggatttg gcaaaaaggt tttcggacac aattaccaaa
     3181 gtattgttgg cgtacataga tatattcatt gaagaagctg gctactccca ggacaatttc
     3241 cctcacggat ttggattcaa cgatgatgac tgctgtcttc tgacacccga gatgtatgag
     3301 ttttttgcat atccgatttt aaagactgtt ttcgataaaa cagcgccaaa tcctgaagac
     3361 tggagatatc agcattcaga ctcggatatg gggcatctgc tgccgattct tgcaaggttc
     3421 aacctgacag gctgcaattt cggcccaaca gttacagtta gggaaatcag gaagcatatg
     3481 cccaataccc gtatagacgg tcagttgtct cctctgactt tcatgagaaa tgatgaagaa
     3541 caaataacca aggaagttct aagagatatt gaaatggcaa aggagaatga cgcaagaggt
     3601 ctcaatctta caaccgcagg ctcaattaac aatggctctc ttcttagcag catgcgtctc
     3661 ataatggcct tgattcaaaa ctatggcaga tattgatcaa taggagaggt gtaaatggaa
     3721 atacgtgttt ctactgtagt aaactatgat gaatacaaaa cctttttcag gttctcaatg
     3781 ttcaggggca agggatacag attcaaatcc ttttcttctg ttctcattat gctttgtgca
     3841 attgcagtat cactgaccgg caccatattt tattacaatc ctttgatgct ttttgcattt
     3901 atattctctc tgctgatgac tgtgactctc ctgataatat ggttttacat gcccaggtat
     3961 tcatatataa acacattcat aacaaaagga tccaaagacg attatgcgtt tttggatgac
     4021 agaattctgg tgaatactgt aactgagcat ataaagagtc agacagaaat cagttacagc
     4081 cagatttata aaatctatga aacaaaaaaa cacatttata tctaccttaa tcctggattt
     4141 gcttacataa tcagcaagaa ggcatttgta aatcacgcag acattgcgga acttagaagt
     4201 ttttttgtag caaagatgcc tgagaaatat attcactgta cattctgata ttttatctta
     4261 atggtacaat cttacgccaa agaggaattt tgaatctttg gcgtattttt tccgcatgca
     4321 ttacttcttc tctcctgtat atcttcgaca caaccccaac cacatgttcg ggaaaaacaa
     4381 tgccgtctgt tcttttgtat gcatcacctt ttgtcacgta tccaaggtcg gtttttctat
     4441 atatcctgcg caagacaaaa atgccttcag caggctcaag cataacaatg tccccaattt
     4501 caagatctct tccatgggag taaacaatct caatcttgtc aattcctttt ctgacaagag
     4561 gttccatgcc acttgttact gctgtaaacc tgacaccttc gcatactttc aaaaggcttc
     4621 taacagctgg gagcaaatcc ttaaccaata tgtccacaag cattttaagc ccctttaatt
     4681 attttccttc taattcttgc cattaatgaa tagagatata acagctgcct gcgaaagatc
     4741 cttagtttcc tccacattgc aaaaaccaaa caccaaccca cactattaca gtcaatgtct
     4801 aaatcccctc tgaatattct ctcaactctg ccaacaatat cgcatacagg gacacttccg
     4861 tcactgaaaa gacatgcatc cccgattgta taaacgttct ccccccttat cttatatatt
     4921 ctgtgaagaa tatatttgct acacattcga aataaaacta tatccccaag cttagccttg
     4981 caaaatgtac cgagcagagt ctttgttgtg ccgaata
//
LOCUS       DAONVY010000110         5003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12177_length_5003_cov_2.914511, whole genome shotgun sequence.
ACCESSION   DAONVY010000110 DAONVY010000000
VERSION     DAONVY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12177_length_5003_cov_2.914511"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLI11_04120"
     CDS             complement(<1..716)
                     /locus_tag="PLI11_04120"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPZ52081.1"
                     /translation="MILAIDIGNTNLVLGAFEEDKLKFVARISSDTNRTEDEMAVIIK
                     SVLENHGAYSSDISGTIISSVVPSLTQTSSKAIKLLTGEYPFVVEPGIRTGINILIDN
                     PAQLGSDLLVDSVAAANLYPKPVIIVDMGTATTFSVVDEDNCMIGGAIMPGVRISVNA
                     LSERTAQLPHINIEAPKKAIGTNTIDCMRSGVVFGAAAMIDGMIDRFEEELGKECFVV
                     ATGGLAEEVCKHTKRKIEYN"
     gene            883..1941
                     /locus_tag="PLI11_04125"
     CDS             883..1941
                     /locus_tag="PLI11_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPZ52082.1"
                     /translation="MADLKLFDQKEKRKQERKLRKQEMKKAKMEAKARKKEKKKGMGY
                     LFLPPEKKSYAWVWILLIIVLIAALVIYVTRDNQQLTVHYAEFEHEQIPEAFDGYRIL
                     QITDLHGKTFGTMQKRLASHINSLEFDMILMTGDYLRSPDDEDYYVIIDLIDSIEKEV
                     PIYYILGETDYRPDREDLDDNWNMCIIPSKKTPLMEKLEERGCSFVYPIQRIEKGGEY
                     IYLTGTKYYEKKFDELGFDSDKHFSICVTHKPINYNVQRRLDDVNQRYLVEVDFDASI
                     SGHTIGGHARLPLLGAFYTDDKGLLPEERYAYGLSVDNHGRLKFISSGLGIPSSFRLR
                     AFNTPEVAIITLKHKTAD"
     gene            1961..2656
                     /locus_tag="PLI11_04130"
     CDS             1961..2656
                     /locus_tag="PLI11_04130"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HPZ52083.1"
                     /translation="MRSKIIWALLLLAVSLALILNITGVVSFPNEKVNLWTFWPVVLI
                     GIGLTSLFSSGSRVISLLFIFFGGYWMYDALGYEAPKMTMELVGAIVLLLIALSILFS
                     AFKKQREARRTNSKNSYSNVFSSGEIEYNDDVFTKGEVSAVFGGTSFDLTNTQINPNG
                     AYLSVQVSFGGIEIFVPDGTNVISKGFIFFGGVSHDRNKKGTKTSNREGIHNGTLTVE
                     VSGAFGGVNIIYV"
     gene            complement(2653..3864)
                     /locus_tag="PLI11_04135"
     CDS             complement(2653..3864)
                     /locus_tag="PLI11_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPZ52084.1"
                     /translation="METKTKKLYILAIYLTLVLILSSCSLLKKDISPTPTIASTQGLE
                     TMEPTPTYTITPTPTPTPYYTPDIPNPTLFPTYVPEGQKIVNPFRIYNSQTYEFDVLN
                     LAHRYSEILKVEVFGKSYMGKNLYVVKLGKGSTKVLVTGTTHARENITTNYIMRTIEE
                     YAISYLNKETYDGYNVYEILNKVTIYYVPLVNPDGLDIVNLLKDPIDDPDNPVFGKLD
                     EYTKVTWKANARGVDLNKNYPFIFEEIEAINPTQRFASQDYHGPYPASEPETYAMINL
                     CESNDFQFCINLHTKGKVMFWRDEGNGVIEGDEKLARLINKKTGYTMYTPTTNPLSYG
                     AAHENWFRYRFKKPAICLELTAGNDGPIPYNLINREKFGGTLYDYYGTRVLDWEKTKS
                     LMLMLAKEYFG"
     gene            complement(3935..>5003)
                     /locus_tag="PLI11_04140"
     CDS             complement(3935..>5003)
                     /locus_tag="PLI11_04140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPZ52085.1"
                     /translation="KTVGAAFDEIFSTTEGRIIVATFSSNIHRVQQIVNASKKFGRKC
                     VICGRSMVNVVDVTKQIGYMDNIDDVLIDIDEIGDYSPEELCIITTGSQGEPMSALAR
                     MAIATHRQIDIVPGDTVIISANPIPGNEKPIAKMIDELFKRGANVIYKALADVHVSGH
                     ACEEELKLIQALVKPKYFVPVHGEYKHMIHHKNIAVGMGIPEDNIFILENGRVLEFDN
                     GAVVNTEETVPAGNILVDGLGVGDVGNIVLRDRRHLSADGLIIVVMTIEASSGKLLSG
                     PDIISRGFVYMRESEDMIAKIREVTKEALARSDDSRKRDWTLKKTNIRDAIHEYVYEQ
                     TRRNPMILPIIMEVDLAKLDY"
BASE COUNT         1354 a          968 c         1083 g         1598 t
ORIGIN      
        1 ttgttgtact caattttgcg tttcgtatgt ttgcagactt cttctgccaa accgccggtg
       61 gcgacaacga aacactcttt accaagctct tcctcaaacc tgtcaatcat gccgtcaatc
      121 atggcagctg caccaaaaac aactcccgac ctcatgcagt caattgtgtt tgtaccaatt
      181 gcctttttgg gtgcctcaat gtttatatgg ggcagttgtg cggttctttc tgacaatgca
      241 ttaactgaaa ttcttacgcc aggcataatt gctccaccaa tcatacagtt atcctcatca
      301 accacactga aggttgtagc agtgcccata tccacaataa taacaggctt tgggtacaga
      361 ttggctgctg ctacggaatc cacaagcagg tcgctgccca gttgtgcagg gttgtcaata
      421 aggatattga tacctgttct aattcctggt tcaaccacaa aagggtattc tcccgtcagc
      481 agctttattg cttttgaact ggtttgagta agagatggaa ccacggaaga tatgattgtg
      541 cctgagatgt cagagctgta tgcgccgtgg ttttcaagta cagacttgat aattactgcc
      601 atttcgtctt cagttctgtt tgtatctgaa gatatgcgcg ctacaaactt gagtttatct
      661 tcctcaaatg cgcctaagac cagatttgtg ttacctatgt cgattgctaa tatcaatttt
      721 atccctcctt gctactgttc ccaaaaggat acagtgcaaa aataaatata gatgattttt
      781 atgaacatta caagtacatt ttttaaacaa tgtttgattt gactaaaagg gttgctgtgt
      841 gctaacatta ttctgcataa atttaatgta aagaggattt gtatggcaga tctgaagctg
      901 tttgatcaga aggaaaaaag gaagcaggaa agaaaactta gaaagcagga aatgaagaaa
      961 gcaaagatgg aggcaaaagc ccggaagaag gaaaaaaaga aaggaatggg ctatctgttc
     1021 ctgccacctg agaagaagag ctatgcctgg gtttggatcc ttttaattat tgtcttgatt
     1081 gcggcactgg tgatttatgt tacaagggac aatcagcagc ttacagtaca ctatgcagaa
     1141 tttgagcatg aacagatacc cgaggctttt gatggttaca ggattctgca gataacagat
     1201 ctacatggca agacttttgg caccatgcag aaaaggcttg catcacacat caacagtctt
     1261 gagtttgaca tgatacttat gaccggagac tacttacgaa gtcctgatga tgaagattat
     1321 tatgtaataa ttgaccttat agattccatt gagaaggagg ttcctatata ctatattctt
     1381 ggtgaaacgg attacaggcc tgatagagaa gatctggatg ataactggaa tatgtgcatt
     1441 ataccgagta aaaagactcc gctgatggaa aaactggagg aaagaggatg ttcttttgtt
     1501 tatccaatac aacgaattga aaagggtgga gaatacattt accttacggg taccaaatat
     1561 tatgagaaga agtttgacga acttggattt gattccgaca aacacttcag catatgtgta
     1621 acccacaagc cgataaacta caatgtgcag agaaggcttg atgatgtgaa tcaaaggtat
     1681 cttgttgagg ttgattttga tgccagtatt tcagggcaca ccattggcgg gcatgcaagg
     1741 cttccgcttc tcggagcttt ttatactgac gacaagggtc ttctgcctga agaaaggtat
     1801 gcatacggtt tgtcagtgga taatcatggc aggctgaaat tcataagttc aggtttgggg
     1861 attccttctt ctttcagact tagggcgttc aacacacccg aagtagcaat tattacatta
     1921 aaacataaaa cagcagacta ataataaaag gaggcttgcc atgagaagca agattatttg
     1981 ggctttattg ttactagccg tatcattggc tctaatacta aatattaccg gagttgtatc
     2041 ttttccaaat gaaaaggtta atctgtggac cttttggcct gttgtgctaa ttggaatagg
     2101 tttgacatca ctgttcagtt caggttccag agtgatatcc cttttgttca tattctttgg
     2161 cggatactgg atgtatgacg cccttggtta tgaagctcct aaaatgacaa tggaacttgt
     2221 tggtgcaata gtgcttttgc tgattgcttt gtcgatactt ttctctgcct tcaaaaagca
     2281 aagagaagca agaaggacaa acagtaagaa ttcatacagt aatgtgtttt catcggggga
     2341 aattgaatac aacgatgatg ttttcacaaa aggggaagta tctgcagtgt ttggtggaac
     2401 cagctttgat ttgaccaaca cccagattaa tcctaatggt gcatatttgt ctgtacaagt
     2461 cagttttggc ggtattgaaa tattcgttcc ggatggaacg aatgtaattt caaagggttt
     2521 catattcttt ggcggagtat cccatgaccg gaacaaaaaa ggcaccaaaa cttcaaatcg
     2581 cgaagggata cataacggta cccttactgt ggaagtttcc ggcgcctttg gaggagttaa
     2641 cattatttac gtctagccga agtattcttt ggccaacatt agcattaggg atttggtttt
     2701 ttcccagtca aggactcttg ttccgtagta atcatacaat gtacctccaa atttctctct
     2761 gtttatgaga ttgtatggga taggaccatc attacctgcg gtaagctcaa ggcatatcgc
     2821 aggttttttg aacctgtatc taaaccagtt ttcatgggct gctccataac taagtggatt
     2881 ggtggttgga gtgtacatgg tgtaaccggt cttcttgttt atgagtcttg ccagtttttc
     2941 atccccttca attactccgt tcccttcatc cctccagaac atgactttgc cttttgtatg
     3001 cagatttata cagaattgaa aatcgttaga ctcacatagg tttatcatgg catacgtttc
     3061 aggctctgac gcagggtagg gaccgtggta gtcctgggat gcaaatcttt gtgtgggatt
     3121 tattgcctca atttcttcga atatgaaggg gtagtttttg ttaaggtcaa ctcctcttgc
     3181 gtttgctttc caggttacct ttgtgtattc gtccagtttg ccgaataccg gattatcagg
     3241 atcatctatc ggatctttaa gaagattcac aatgtcaagt ccgtccggat ttacaagagg
     3301 tacatagtat atggttacct tgttgagtat ttcatagacg ttgtagccat cgtaagtttc
     3361 cttgttgaga tagctgattg catactcttc tatggtacgc atgatatagt ttgtggttat
     3421 gttttccctt gcatgtgttg ttccggttac caataccttt gtgcttcctt ttcccagctt
     3481 tactacataa aggttcttgc ccatgtatga ttttccaaat acctcaactt ttaaaatctc
     3541 tgaatacctg tgagcaagat tcagaacatc aaactcatag gtttgagagt tgtatatcct
     3601 gaaaggattg acaatctttt gtccctctgg gacataggtt gggaataatg tcggatttgg
     3661 gatgtctgga gtatagtatg gagttggcgt gggtgttgga gtaatggtgt aggttggagt
     3721 tggctccatt gtttccagac cttgggtaga agcaattgtt ggagtggggg atatatcttt
     3781 tttcaacagt gagcaggagg agagtataag tacaagggtt aaatatattg caagtatgta
     3841 tagtttttta gtttttgttt ccataaaaca cctcaaatta taaagaccgg actgttttca
     3901 caatccggta ctatggtctc ttatcctata tttattaata atccagtttt gccaagtcga
     3961 cttccatgat tatgggaagt atcattgggt tgcgccttgt ctgttcgtat acatattcat
     4021 gtattgcatc cctgatattt gtcttcttaa gagtccagtc gcgtttcctt gagtcatcgg
     4081 atcgggcgag cgcttctttt gtaacttccc tgattttcgc aatcatgtct tcagattccc
     4141 tcatgtagac aaaacctctt gagataatgt ccggacctga aaggagtttt cctgaacttg
     4201 cttctatggt cattacaacg attatcaagc cgtcggcaga aagatggcgc ctgtctctca
     4261 agacaatatt accaacatcg cctacgccaa gaccgtcaac aagaatgttg cctgctggta
     4321 cagtttcttc tgtgttcaca accgcaccgt tgtcaaactc aagaactctt ccgttttcca
     4381 gaatgaatat gttatcttca ggaattccca tacctactgc aatgttcttg tggtgtatca
     4441 tgtgcttgta ttcaccatgc acaggtacaa aatactttgg ctttactagt gcctgtataa
     4501 gttttaattc ttcttcgcag gcgtgtccag atacgtgtac atcagccaat gctttgtaga
     4561 ttacgtttgc accgcgtttg aagagctcgt ctatcatttt ggcaattggc ttttcgttgc
     4621 ctgggattgg gtttgctgaa atgattacag tatctccagg aactatgtct atctgcctgt
     4681 gggttgcaat agccatacgt gcaagagcgc tcattggttc tccctggctg cccgtggtta
     4741 tgatgcatag ttcttcaggg ctgtagtcgc caatttcatc aatgtcaata agcacatcat
     4801 ctatattgtc catataccca atttgctttg taacgtcaac aacattcacc atactgcgac
     4861 cgcaaatcac gcattttctt ccaaacttct ttgaagcgtt tacaatctgt tgtacacggt
     4921 gtatgtttga tgagaaggtt gcaacaatta ttctgccttc ggtagttgaa aaaatctcat
     4981 caaatgccgc tccaacggtt ttt
//
LOCUS       DAONVY010000111         5002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12179_length_5002_cov_3.415808, whole genome shotgun sequence.
ACCESSION   DAONVY010000111 DAONVY010000000
VERSION     DAONVY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12179_length_5002_cov_3.415808"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..615)
                     /locus_tag="PLI11_04145"
     CDS             complement(88..615)
                     /locus_tag="PLI11_04145"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HPZ52086.1"
                     /translation="MMKKDGLDARSIIMLVVIAIIIIIIGFLVYLYYLGDNVHWLARK
                     NPSETPGVTETPEPTATEEPAPTPYPVNKEGNTIKERFPVPEGYERVPVEPGSFAEFL
                     RNHPLKNYGVPAYTYEGIINEEASIQGVFNQDITPRDLQQCADAIMRLWAEYLYEREE
                     YGRISFNFLSGFECD"
     gene            complement(617..1576)
                     /locus_tag="PLI11_04150"
     CDS             complement(617..1576)
                     /locus_tag="PLI11_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52087.1"
                     /translation="MKLFKFLDFTKDGKGIEKDAPQKRAFFRFWSRFWEKRYRLIGAN
                     FLYLPSAIISLFVATLVFLVAASLFVTLEGSDNAISQFLQNRESATEMARQTIIIVIA
                     FTAIITTVVPIFAVGPFYAGFTYLLRSIVTENPVFLWHDFKTKARSNFKLGLKVGLTN
                     FVAGFFLMVDAAAYMAISNNSQGQFSNVPSFILFIAIAVILFLSLLLMMVNLYAYPIM
                     VTFNVTYKQLYKNAIILSLIRWIPNLLILILNAVLIALPFLFMFGNSTLVIPTVLYIF
                     ITPAFIGFLNNFYVNNTIKKYLIDNESADKSKETTKEAEGDVI"
     gene            1742..3472
                     /locus_tag="PLI11_04155"
     CDS             1742..3472
                     /locus_tag="PLI11_04155"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPZ52088.1"
                     /translation="MPYDGIVANAAVWELSGLLAGGRIEKVYQTERDEITLLIHSMGE
                     RFRLLASANATYPRLHLTIGKKENPMNPPPFCMVLRKHLQGGRVVDITQSGYDRIITL
                     TIVKTDEMGDLVKKHLVVEIMGRHSNIILVNDSKTIIDSIKHVDANISSVREVLPLRP
                     YVLPPQMEKQSPEFIDFEKLFNEKPAQRLDKHLVNCIGGFSPFLAMAICNNAEVDPFK
                     NISHLTDCDKLRLKETITETTNNIKERKYSPAIIHGDKVDFHVLSVAHGERTVLFNTV
                     NLMLDEYYTKKDIDEKLNAIRANIQKSVNTAIDRNTKKLAIHEETIREAADYDKYRIY
                     GELLFSNIYNLKEGKYAEVLNYYEDPPIHVKIELDEERSIKNNANRYFNRYKKLKSSY
                     ENALNLLEEVKNELFYLENVKTHLENATDFETLEDIRQELYEGGYVKFQTKSRTAKKL
                     ETKSKPIHVVSEEGYDIYIGRNNKQNEELSLRFASQNDLWLHVKDAPGSHVIVRTGAK
                     NGVVTQKVIEEAAAYAAFYSSHRTSTNVIVDFTRVKHVRKTPKSKPGMVSYTNHKSIV
                     VKPQEASSQS"
     gene            complement(3456..>5002)
                     /locus_tag="PLI11_04160"
     CDS             complement(3456..>5002)
                     /locus_tag="PLI11_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52089.1"
                     /translation="AHYDHLRGAYRFTMDGSNFNKAYYREPDTHYTATIKIKSDGNDR
                     DFYIWMNTDAGGLECAGLLDDTKTLIPIPMQVCKNFKGEIEEPFYDPIDPAYGDTFFP
                     LRLNANEEAEFTLLNLYQNWGNYPLKQLSSIQFHISYYHLSTGVTESNCIAPYFVYGK
                     DGWTLPDFRGASGIMWDSQPQFNSVGRLYFLSYQKDRNIGSEYTGSHIRCAGQTYVDM
                     DYSYVSDCGSYKYTLRHVEFPQLDENRTYYSLSLEFLKNLTIDNVREDFTIFAFDGRF
                     TYFKQTGYLDKDNNPKVERVDTLAPGSRIFTLGTENPYFSYFDLHNGENVMNFGFILK
                     SSNITIGGKKFKGNFAYRDSFDGNLNTGAISLDLGKTTFKKGDKIQMDFILLPYGSHN
                     DKHDDNVRMVREDSALNPLKIEAITGTVVKDAYIPRIKCENNRAEFRITGGRNNNVVC
                     VDGFTNIKKPNIYELVDGEYVPYEYSVHEYDGYSVHFNMDGTYSFSFVFEMESPSTER
                     VFKIEN"
BASE COUNT         1573 a         1057 c          984 g         1388 t
ORIGIN      
        1 gtaactgtaa tcttcgccag catcggtttc aacccatgaa acttcgtttc catcaatttt
       61 cacacgcttg ccgctagccc aggatactta gtcgcattca aaaccgctta ggaagttaaa
      121 cgatattctg ccgtactcct cacgttcata aaggtattca gcccatagcc tcattatggc
      181 atcagcgcac tgctgcagat cccttggagt aatatcctgg ttgaatacgc cttgaatgct
      241 tgcctcttca tttataattc cctcatacgt atatgcaggc acgccatagt tcttaagagg
      301 atgatttctt aaaaattcag caaaactgcc tggctctaca ggaactcttt catatccctc
      361 aggaacagga aacctctcct taatggtgtt tccttccttg tttacaggat atggagttgg
      421 tgcaggttcc tccgtggcag tgggctccgg agtttctgta acacctggag tttcacttgg
      481 gtttttccta gcaagccagt gcacattgtc ccctaaatag taaagataga ctaaaaatcc
      541 tattatgata ataattatgg cgattaccac tagcatgata atgcttcttg catcgagacc
      601 atcttttttc atcatgttaa atgacatccc cttccgcttc ttttgtagtt tctttggatt
      661 tgtctgcgct ttcgttatct atgagatact ttttaatagt attattaacg taaaagttgt
      721 ttaagaaccc aataaatgca ggagtgataa atatatacaa aaccgtgggt attaccaagg
      781 tagaattgcc aaacataaac aggaacggca gagcaatcaa taccgcattc aatatgagta
      841 tcaaaaggtt aggtatccac cttatcaatg acagtataat tgcattcttg taaagctgtt
      901 tgtacgtgac attgaaggta accatgatgg ggtatgcata taaatttacc atcataagaa
      961 gaagcgacaa aaacaggata actgcaattg caataaacag tatgaaggaa ggtacattgg
     1021 aaaactgtcc ctgactgttg ttgctgattg ccatgtaagc tgccgcatcc accataagga
     1081 aaaaacctgc cacaaagttt gtcagcccca ctttcaatcc tagtttgaaa ttgcttcttg
     1141 cctttgtttt gaaatcatgc cacagaaata caggattctc cgtgacaata cttctgagca
     1201 ggtatgtaaa tcctgcgtag aaaggaccaa ctgcaaaaat cggaacaaca gtggttatga
     1261 ttgctgtaaa tgctattaca ataattattg tctgccttgc catttccgtt gcggattccc
     1321 tgttctgaag gaactgtgaa atggcattgt ccgaaccttc aagcgtcaca aacaaagatg
     1381 ccgcaaccag gaataccaat gttgcaacaa aaagagaaat tattgcgctg ggcaaatata
     1441 aaaaattcgc accgattaac ctatatcttt tttcccagaa tctgctccaa aacctaaaaa
     1501 atgctctttt ctgaggagcg tccttttcta taccttttcc gtcttttgta aaatctaaaa
     1561 atttgaacaa tttcaatatt aacactccaa ataatacttg tcttgcgcac aattaattat
     1621 accatacgtt tgactttatc cacaacacaa aaaatggttc ttttcaagaa cgtttttgct
     1681 ttgtacaaaa gtatgcatta tggtaacata tattgtacaa gttattaaag gagttggaaa
     1741 catgccctat gatgggatag tcgcaaacgc agcagtatgg gaattgtcag gactgctggc
     1801 aggaggaaga atagaaaaag tttaccaaac ggagagagat gaaataacat tactcataca
     1861 ttccatgggg gaaagattca ggctgcttgc cagcgcaaac gccacctacc ccagacttca
     1921 tcttacaatc gggaagaaag aaaatcctat gaatccacca cctttctgca tggtgctgag
     1981 aaagcatctt cagggcggaa gagttgtgga cataacccag tcaggatacg atagaatcat
     2041 aactcttacc attgtaaaaa cggatgaaat gggcgatctt gtaaaaaagc acctggttgt
     2101 tgaaataatg ggaaggcaca gcaatataat tctcgtaaac gattccaaaa caatcataga
     2161 ctcaataaag catgtagacg caaatattag cagtgtaagg gaagtccttc cgctgcgtcc
     2221 ttatgttctg cctccgcaga tggaaaagca aagcccggaa ttcatagatt ttgaaaaact
     2281 tttcaacgaa aagccagcac aaagattgga caagcatctt gtgaactgca taggggggtt
     2341 cagtcctttc ctggccatgg caatttgcaa caatgcggag gtggatcctt ttaaaaacat
     2401 ttcacatcta acagattgtg ataaacttag actgaaagaa acgataacgg aaacaacgaa
     2461 taacataaaa gaaaggaaat actctcctgc aataatccat ggagacaagg tggatttcca
     2521 tgttctttct gttgctcatg gagaaagaac agtcttgttc aacactgtga atctaatgct
     2581 ggatgagtac tatacaaaga aggacataga tgaaaaactt aacgcgatca gagcaaatat
     2641 tcaaaaaagc gtcaatacgg caattgatag aaacacgaaa aaacttgcta tacatgaaga
     2701 aaccataaga gaagcagccg attatgacaa gtatagaata tacggagagc ttctattttc
     2761 aaatatatac aaccttaagg aaggtaaata tgcggaagtt ttaaactact acgaagaccc
     2821 gccaatccat gtaaagatag agttggacga ggaaagaagt ataaaaaaca atgcaaacag
     2881 gtattttaac aggtacaaaa agctaaagag ctcgtatgag aacgcattaa acctccttga
     2941 agaggtaaag aacgaactct tttaccttga gaatgttaaa acccatcttg agaatgctac
     3001 cgattttgaa actttggaag acataagaca ggagctatat gaaggcggat atgtaaaatt
     3061 ccaaactaaa agcagaacag caaagaagtt agaaacaaaa tcaaaaccca ttcatgttgt
     3121 ttcagaagaa ggttacgaca tatatattgg cagaaacaac aagcaaaacg aagagctttc
     3181 gctcaggttt gcctcccaga acgacttgtg gctgcacgta aaagatgctc ctggttccca
     3241 cgttatagtg agaacaggag caaagaatgg tgttgttact caaaaagtta ttgaggaagc
     3301 tgcagcgtat gccgcctttt acagcagtca tcgtacctca accaatgtta tcgtcgactt
     3361 cacacgagtc aaacatgtgc gaaaaacacc aaaatcaaaa cccggcatgg taagctacac
     3421 aaaccacaaa agcattgtgg ttaagcccca ggaagctagt tctcaatctt gaaaactctt
     3481 tcagtggacg gactttccat ctcgaacaca aacgagaagc tgtaggttcc gtccatgtta
     3541 aagtggactg aatatccgtc atactcatga accgagtatt catagggcac atattctcca
     3601 tccacaagct cgtatatgtt cggcttcttt atgtttgtaa agccgtccac gcacactaca
     3661 ttgttgttcc ttccaccggt tattctgaac tcagccctgt tgttctcgca ctttatcctt
     3721 ggtatgtagg catccttcac gacggtgcct gtaatagcct ctatcttcaa aggattaagt
     3781 gccgagtcct ctcttaccat ccttacattg tcatcatgct tgtcgttgtg tgaaccatat
     3841 ggaagcaaaa tgaaatccat ctgaatcttg tcacccttct tgaaggtagt cttgcccaga
     3901 tccagagaaa tcgctcccgt gttcaggttg ccatcaaaag aatccctgta tgcaaagttg
     3961 cccttgaact tctttccacc tattgtaatg ttgctgcttt tcagtatgaa tccgaagttc
     4021 atgacattct cgccattatg caggtcaaaa tacgaaaagt atggattctc tgttcccagt
     4081 gtgaaaatcc ttgaacccgg cgccagagta tcaactcttt ccacctttgg attattgtct
     4141 ttgtcaagat agccggtctg cttgaaatat gtaaatctgc catcaaaggc aaaaatggta
     4201 aagtcctccc tcacattgtc aatggtcaga ttcttcaaga actccagcga cagagagtag
     4261 tacgttctgt tttcatccaa ttgagggaat tccacatgtc ttaatgtgta tttatatgaa
     4321 ccgcagtcgg aaacatagct gtagtccatg tctacataag tctgccctgc acagcgaata
     4381 tggcttccgg tatactcgct gccaatattt ctgtcttttt gataagataa aaagtacaac
     4441 ctgcccacag agttgaactg tggctggcta tcccacatta ttccgctagc tcctctgaag
     4501 tcaggcagag tccagccgtc cttgccgtat acaaaatatg gagctatgca gtttgattcc
     4561 gtgacacccg ttgagaggtg gtaataagag atgtggaact ggatggagga caattgtttc
     4621 aaaggatagt tgccccaatt ctgatagaga ttcaaaaggg taaactccgc ttcctcgttt
     4681 gcattaagtc tcaacgggaa aaatgtgtca ccgtatgcag gatcaattgg gtcgtaaaac
     4741 ggctcctcaa tctcgccttt gaagtttttg caaacctgca ttggaatcgg tataagggtt
     4801 tttgtatcat ccaaaagacc tgcgcattca agaccgcctg catccgtgtt catccagatg
     4861 tagaagtccc tgtcattgcc gtctgattta atctttatgg tcgcagtata atgtgtgtca
     4921 ggttctctgt aatatgcctt attgaaattg gaaccatcca tggtgaacct gtatgctcct
     4981 cttaaatgat cataatgagc ga
//
LOCUS       DAONVY010000112         4997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12195_length_4997_cov_4.329219, whole genome shotgun sequence.
ACCESSION   DAONVY010000112 DAONVY010000000
VERSION     DAONVY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12195_length_4997_cov_4.329219"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1115)
                     /locus_tag="PLI11_04165"
     CDS             complement(162..1115)
                     /locus_tag="PLI11_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPZ52090.1"
                     /translation="MMGLTITAEDEKRVKALGFLRNKGTNNFSGRIITVNGKITAAQN
                     KCIAEAAELYGNGIVTFTTRLTVEVQGIPYDKIEDFRTYIAKEGLVTGGTGSKVRPIV
                     SCKGTTCQYGLIDTFGLSEEIHEKFYNGYSNVRLPHKFKIGVGGCPNNCIKPELNDLG
                     IVGQLIPNFDEDFCNGCKKCHVEDVCPINAAKVSEGILHIDENECNNCGRCVEKCPFD
                     TIEDGTVGYKVYIGGRWGKRVAHGIPLGKIFTDKEEVMSVIEKTILIYREQGKTGERL
                     SETIERLGFENVEAQILSNEILDRKQEILEANLHLTGGATC"
     gene            complement(1134..2756)
                     /locus_tag="PLI11_04170"
     CDS             complement(1134..2756)
                     /locus_tag="PLI11_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ52091.1"
                     /translation="MKVLIIGGVAAGTKVAAKLKRDNFELDVTILTKSKDISYAGCGL
                     PYYVGNVIQEANKLIVNTPDSFHKLTGVNVLVETEVTQVNPEEKYVEAICLKTQEKMR
                     CEYDKLVIASGAEAVRPNIEGADLDNVFFMRTPEDAYVLRDAIETGNIKRAVIVGGGY
                     VGLEIAENLNSQGVRAVVIDMAKHILPGFDDEISEYVENHLAEQGIPILTETRLEAIL
                     GDGKVEKIKTDRRAMKADAVILCVGIKANTEYLKDSDIDLMPNKTVKVNEYLETNVKD
                     IYAVGDCATVINRVTNKPYWSPMGSTANIAGRIAAKNIAGKKKAYPGVLGTSVVKLPE
                     LNAGRTGLTEKEANENGYNAASVVTVVDDKVHYYPDASSFIVKMIADKDTKKLLGLQV
                     LGKGQVDKMVDIAVAAITMGATLEDVENMDLAYAPPFSTAIHPFSHTVNILLNKLNGE
                     FETVTPADFSKEKAEGFKLIDASLSPSIDGAQYLDLAKIKGEVDGLGRDEKLLLVCAK
                     GKRAYMLQKRLKHFGYTNTKVLEGGTTFNDLE"
     gene            2867..3808
                     /locus_tag="PLI11_04175"
     CDS             2867..3808
                     /locus_tag="PLI11_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPZ52092.1"
                     /translation="MLKKGDVMTIRHLKIFIEVVDSGKMSTAAENLYISQPTVSQAIK
                     ELEEHYGGLLFERLSKKLYITEMGEKLLSYARNVVKMFDDMEEAMLQDAYLKRIRIGA
                     THTVGDCLLGDVINALREINPNTEIYSYVSNTKNIEEKLLRSELDIGIVEGSVKSPDL
                     ISIPELNDFLVLICSTKHPFAKRNMVKLEELKSESFAMREEGSGTRELFERYMQEKGM
                     PIRIALEGNSSDAIKKSVIENGFLAVISVRLVEEEVKNGQIHVITNEDKGWDRFFSVV
                     YHKNKVMTEEMKNLIGIVDHYKNVDVLEGISTGRLIK"
     gene            3825..4163
                     /locus_tag="PLI11_04180"
     CDS             3825..4163
                     /locus_tag="PLI11_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPZ52093.1"
                     /translation="MDKGRRKELIEMYKNRKPEMGVISYRCKATGNVFLGISKETRAD
                     FNSNNFKLNAKLHPNKHLQELWNKYGADSFEMNVVKFLEYEEPGDDHTEELELLLEIC
                     LSEDPDAVKL"
     gene            4437..>4997
                     /locus_tag="PLI11_04185"
     CDS             4437..>4997
                     /locus_tag="PLI11_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPZ52094.1"
                     /translation="MVKYVCSVCGYEYEESNEKPWNELPEDWVCPLCGASKSEFVKKG
                     EAAPVQEEKEVPLIEVSTEVDELSPLELSALFSNLARGCEKQYKQEESELFKELAGYF
                     KKVAAPYESPGFDKLLEMVEKDLSEGMPNANAVAAEEKDRGALRALTWSEKVTRMLKS
                     ILTRYMKEGDAMLENAEVHVCTICGFV"
BASE COUNT         1413 a          923 c         1055 g         1606 t
ORIGIN      
        1 gacactcctc aaaatgtttt tgaaagcaaa ttgattgacg aagtgtttgg tataacttct
       61 tctcaagtta tggttggaaa gaatgaaata cagtatgttt tctgtttgaa ctaaagaaaa
      121 aactttcatc atgcgatgaa agtttttctt ttagggggtt attagcaggt agcgccgcct
      181 gtcaaatgca gatttgcttc aagtatttcc tgctttctgt caagtatttc attggagagt
      241 atttgagctt ccacattttc aaaaccaagt ctttcgatgg tttctgacag acgttctcct
      301 gtcttgcctt gttctctata tataagtatg gttttttcaa ttacactcat aacctcttct
      361 ttgtctgtaa agatctttcc aagaggtatt ccgtgagcaa cacgttttcc ccatcttccg
      421 cctatgtaca ccttgtagcc gacagtaccg tcttcaatag tgtcaaaggg acatttttca
      481 acgcagcgtc cacagttgtt gcattcgttc tcgtcaatgt ggagaatacc ctccgatacc
      541 tttgcagcat tgattggaca tacgtcttcc acatggcact tcttgcatcc gttgcagaag
      601 tcttcatcaa agttaggaat gagctgtcct acgatgccca agtcgtttaa ttcaggcttt
      661 atgcagttgt tgggacaacc gcctacacct atcttgaact tgtgaggaag ccttacattg
      721 gaatatccgt tgtagaattt ttcgtgtatt tcttcagaca atccaaaagt gtcaataagt
      781 ccgtactggc aggtggtgcc tttgcaggac actatgggtc ttacctttga accagtacca
      841 ccagttacaa gaccttcttt tgcaatgtac gttctgaagt cttcgatttt gtcataagga
      901 ataccttgta cttccactgt aagtcttgtg gtaaaggtaa ctattccatt gccgtacaat
      961 tcggcagctt cagctatgca tttgttttga gctgcagtta tttttccgtt tacagttata
     1021 attcttcctg aaaagttgtt tgttcctttg ttgcgcaaaa atcccaaagc tttgactctt
     1081 ttttcatctt ccgcagttat tgttaatccc ataatcttaa aacctccata attttattcc
     1141 aaatcattaa atgttgttcc gccttcaagc acttttgtgt ttgtatatcc aaaatgtttg
     1201 agtctctttt gaagcatgta tgctcttttc ccttttgcac ataccagaag aagtttttca
     1261 tctcgtccaa gaccatccac ttctcctttt atctttgcca aatccaagta ctgtgcgcca
     1321 tcgatacttg gacttaagga tgcgtcgatt aacttaaatc cttccgcctt ttctttagaa
     1381 aaatctgcag gtgtgacagt ttcaaattct ccattcaatt tatttaaaag tatgtttaca
     1441 gtgtgagaga aaggatgtat tgctgttgag aaaggaggtg cgtatgccag atccatgttc
     1501 tcaacatcct caagggttgc tcccatggtt attgcagcta ccgcaatatc aaccatcttg
     1561 tcaacctgtc ctttacccaa aacctgcagt cccaataatt tcttcgtatc cttgtctgct
     1621 atcatcttta caataaagga tgaagcgtca ggatagtagt ggactttgtc atccacaaca
     1681 gttacaacac ttgctgcatt gtatccattt tcatttgctt ctttttctgt cagtcctgtt
     1741 cttccggcat taagctctgg cagcttcaca acagaagtac ccaatactcc tggatatgct
     1801 tttttctttc cagcaatgtt tttggcagcg atgcggcctg caatgtttgc agtagaaccc
     1861 atgggcgacc aataaggttt gtttgttacc ctgtttatta ctgtggcgca gtcgcctact
     1921 gcataaatat cttttacgtt tgtttccaga tactcattta ctttcactgt cttgttgggc
     1981 attaaatcaa tgtctgaatc tttcagatac tcagtatttg cttttatgcc aacgcagaga
     2041 atgacagcgt ctgccttcat ggctcttctg tccgttttaa tcttttctac ttttccatca
     2101 cctagtatgg cttcaagtct tgtttctgtg aggattggaa taccttgttc tgcaaggtgg
     2161 ttttctacat actctgaaat ttcatcatca aagcctggca ggatgtgttt tgccatgtca
     2221 ataaccacag ctctaacccc ttgtgaattc aggttttctg caatctcaag gcctacatat
     2281 cctccgccga ctattaccgc ccttttgatg tttcctgttt caatggcatc tctcagcaca
     2341 tatgcgtcct cgggagttct cataaagaat acgttatcaa gatctgctcc ttcgatgttt
     2401 ggtcttacag cttcagctcc cgaagctatt accagtttgt cgtactcgca gcgcatcttt
     2461 tcctgtgttt tcaaacaaat ggcttccacg tatttttctt caggatttac ttgtgttact
     2521 tccgtttcga caagtacatt aactcctgta agtttatgaa aactatcggg tgtgtttacg
     2581 attagcttgt ttgcttcctg aattacgttt ccaacgtagt atggaagacc acatcctgcg
     2641 taggatatat ctttactttt tgtcagtatt gtgacgtcga gctcgaaatt atcacgtttc
     2701 agttttgcag cgaccttggt gcctgcggca actcctccaa ttataagcac tttcattaaa
     2761 tgcacctctt tgtttagaat gaatttatct tctatttaaa tatatcactt gtgagattaa
     2821 taataaaatg atattatctg attaaagtca taggtaaaac ctatagatgt tgaaaaaagg
     2881 tgatgttatg acaatcaggc atttaaagat ttttattgag gttgtggaca gcggcaaaat
     2941 gagtacagct gcagaaaatc tatatatctc ccagccgaca gtaagccagg ccataaaaga
     3001 gctggaggaa cattatggcg gtctattgtt tgagaggctg tctaaaaagc tttacattac
     3061 cgaaatgggt gaaaagcttc tttcttatgc aaggaatgtg gtaaagatgt ttgacgacat
     3121 ggaagaagcc atgctccagg atgcatattt aaaaagaata aggattgggg caacgcacac
     3181 tgtaggtgac tgcctccttg gggatgtcat aaatgcttta agagaaatca accccaatac
     3241 cgagatatat tcttacgtaa gcaatacaaa gaatatagaa gaaaagcttt taaggtctga
     3301 attggatata ggtatagtgg aggggagtgt aaaaagtcct gatttgattt caattcctga
     3361 actaaatgac tttctcgtcc tcatttgcag tacaaagcat ccttttgcaa agaggaatat
     3421 ggtaaagctt gaagagctga agagcgaaag ttttgcaatg agggaagagg gaagcggaac
     3481 cagggaattg tttgaaagat acatgcagga aaaaggaatg cctattagaa ttgcactgga
     3541 aggaaacagt tccgatgcca tcaagaaatc cgttattgaa aatgggtttc ttgcagttat
     3601 ttctgtaagg cttgtggaag aggaggtaaa gaacggtcaa atacatgtta taacaaacga
     3661 ggataaaggc tgggacaggt tctttagtgt tgtgtatcat aagaacaagg tcatgactga
     3721 agaaatgaag aatttgatag gaatagttga ccattacaaa aatgttgatg tgcttgaggg
     3781 aattagtacg ggaagattga ttaagtaaac tatgaggtga tgatatggat aaaggaagaa
     3841 gaaaagaatt gattgagatg tataaaaaca ggaaacctga gatgggtgta atatcttaca
     3901 ggtgcaaggc aacgggaaat gtgtttttgg gtatatccaa ggagacgaga gctgatttca
     3961 acagcaataa tttcaagctt aatgcaaaac ttcatccaaa caagcatctt caggaacttt
     4021 ggaacaaata cggcgctgat agttttgaaa tgaatgttgt aaagtttttg gaatacgagg
     4081 aacctggtga tgaccatacg gaggaattgg agcttttgct tgaaatatgt ttgtctgaag
     4141 acccggatgc tgtgaaattg taactatatt aagttattga cttttgtcgg aaaatgaggt
     4201 aaaatcgtat tgtagttata taaaaaatgt aatggacgga gcaatatttg tgaagtattc
     4261 gtttgagaaa ttgaagcaga aattgtatag aagtattatc ggtctgccat tgccgagact
     4321 gaaggattta acctgccttg attatgttaa cccatgaaga tatatttctt catgggtatt
     4381 tttgctttgt atatttagtt aggattaaat ataaatatta atgaggaggt tgtacgatgg
     4441 tcaaatatgt atgttctgta tgcggttacg aatatgaaga atccaatgaa aaaccgtgga
     4501 atgagctgcc ggaagattgg gtgtgcccgt tatgtggagc atccaagtcg gagtttgtaa
     4561 agaagggcga agcagcacct gtacaggaag agaaagaagt acctttaatt gaggtatcaa
     4621 ctgaagttga cgagctttct ccattggaac tcagtgcgct gttttcaaat cttgccagag
     4681 gatgtgaaaa gcagtacaaa caggaagaat cggaactttt caaggaactg gccggatatt
     4741 tcaagaaggt tgctgctcca tatgaaagtc caggatttga caagctgctt gaaatggtgg
     4801 agaaggattt atcagagggt atgccgaatg caaatgctgt agctgcagaa gaaaaagaca
     4861 ggggtgcttt gcgtgcatta acctggagtg aaaaggtgac ccgcatgctc aagtcaattc
     4921 tcacccgata tatgaaagag ggagatgcca tgcttgagaa tgctgaggtt catgtgtgta
     4981 ctatttgcgg atttgtc
//
LOCUS       DAONVY010000113         4977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12254_length_4977_cov_3.682852, whole genome shotgun sequence.
ACCESSION   DAONVY010000113 DAONVY010000000
VERSION     DAONVY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12254_length_4977_cov_3.682852"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /gene="acnA"
                     /locus_tag="PLI11_04190"
     CDS             complement(<1..2331)
                     /gene="acnA"
                     /locus_tag="PLI11_04190"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006318333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HPZ52095.1"
                     /translation="MDRNVFNVKRYFETGGKRYNYYSLKALEENKNINVKRLPYSIRV
                     LLESVLRQYDGWSINEEHVLNLVNWNTDKFDERIDVPFKPARVLLQDFTGVPAMVDFA
                     SMRKAIADIGGDPKCINPNRKADLVIDHSVQVDKYASGDALSFNMDIEFERNEERYRF
                     LSWAQKAFSNFRVVPPATGIVHQVNLEYLAQVVFVDEDEDEILAYPDTLVGTDSHTTM
                     INGLGILGWGVGGIEAEAGMLGKASYFPVPDVVGVKLAGRMKEGATATDLALLVTHVL
                     RKHGVVGKFVEYFGEGVSTLSLADRATVSNMAPEYGATCGFFPVDEETLNYLKLTGRK
                     KEHIELVERYCRENLMFRDSGSVPEYSEIVEIDLGEVEACLAGPKRPQDIIPLSLMKE
                     EFDQSITREKGYRGFGLSENIVMDGDRSSIKNGSVVIAAITSCTNTSNPFVLIGAGLL
                     AKKACELGLEVPPHVKTSLAPGSKVVTAYLRQSGLLEYLERLGFGVVGYGCTTCIGNS
                     GPLRREVEDAIKENDLLVASVLSGNRNFEGRIHPLVKANYLASPPLVVAYALAGSMTV
                     DLYKEPLGRDKNGQDVYLKDIWPTNREIEEIIQKVVASDLFAKEYEDVYEGNKRWNEI
                     ADDSGELYGFDEESTYIQNPPFFEGLKLEVEKIKPLENLRVVGMFGDYVTTDHISPAG
                     SISEDSPAGKYLLEKGVKKESFNSYGSRRGNHEVMMRGTLANIRLNNKLAGGKEGGFT
                     RYFPTGEVTSIYNACMRYKQEGVGLVVIAGEGYGMGS"
     gene            complement(2442..3551)
                     /locus_tag="PLI11_04195"
     CDS             complement(2442..3551)
                     /locus_tag="PLI11_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPZ52096.1"
                     /translation="MDWRRELRNNVTTAEEVFQCLNIEVSKKDLHKYNEIIDKFPMSV
                     SQYYLSLINPEDEDDVIRRMCVPSINETDLSGSFDTSGEKDNTVMTGLQHKYRQTALV
                     LSTNRCAMYCRHCFRKRLVGVNGDEITKEFDNIINYIREHKEINNVLISGGDAFLNNN
                     KKIEEYLEALCDIDHLDIIRFGTRTPVVLPSRIYDDMELLDILDKYRRKKTIYVVTHF
                     NHSREITKESKRAIRCLLDRGIIIKNQTVLLKGINDDGETLAKLTNNLTRIGVVPYYV
                     FQCRPVKGVKNQFQVPLLKAERIIKEARKKLNGQGKCFRFIMSTERGKIEVMGLLKEN
                     KMLFKFHQAKSPEDENRIFVKDIKEDQCWIDVFDS"
     gene            complement(3713..>4977)
                     /locus_tag="PLI11_04200"
     CDS             complement(3713..>4977)
                     /locus_tag="PLI11_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPZ52097.1"
                     /translation="EDEKYVERDRKIDRIITGKYLGYPLMMVLLFVVFWITIRGANYP
                     SAMLARFFFFLEERITYFLELVNAPNWLHGILVLGVYRVLAWVVSVMLPPMAIFFPLF
                     TILEDLGYLPRVAFNLDKYFKKASSCGKQALTMCMGFGCNAVGVTGCRIIDSPRERLI
                     AIITNNFVPCNGRFPTLIAVITMFFTGYLTGLTGSLVSSLILTLVIVMGVVMTFLISG
                     LLSKTILKGVPSSFTLELPPYRRPQVLKVIVRSIFDRTLFVLGRAVIVAAPAGAIIWM
                     MANIHVGGETLLGISADFLDPFGKLLGLDGVIVLAFILGFPANEIVVPIIIMAYMAQG
                     SLIELGSLSELKELLVANGWTLTTAISMMLFTLFHWPCSTTCLTIRKETKSIKWTVLS
                     FAIPTLVGIGVCFIFASTVRLLGLIINT"
BASE COUNT         1442 a         1262 c          782 g         1491 t
ORIGIN      
        1 cgaacccatg ccatatccct ctccagcaat tacaaccaga cccacaccct cctgtttgta
       61 tctcatgcat gcattataga tgctcgtcac ttctcctgtt ggaaaatacc ttgtgaatcc
      121 tccttccttt cctccagcca gcttgttgtt taaacgtatg tttgcaaggg ttccccgcat
      181 cataacttca tggtttcctc tcctggagcc atatgagttg aaggattcct tttttacccc
      241 tttttccaac aaatactttc cggctggact gtcttcgcta attgaacccg cgggtgatat
      301 gtggtctgtg gtaacataat caccaaacat accaacaact cttaagttct ctaacggttt
      361 gattttctca acttccagtt tcaatccttc aaaaaacgga ggattctgaa tatatgtgga
      421 ttcttcatca aaaccataca actccccact atcatccgca atttcattcc agcgtttgtt
      481 tccttcatac acatcctcat attctttggc aaacaagtca ctggctacta ccttctgaat
      541 tatctcctct atttccctat tcgtcggcca gatgtcttta aggtacacat cctggccatt
      601 tttatctcta cccaaaggtt ctttgtacaa atccacagtc atgctccctg ccagggcata
      661 agccacaacc aaaggtggcg atgccagata atttgccttt acaagaggat ggattcttcc
      721 ttcaaaattt ctgttcccag acaaaacaga tgcaaccagc aaatcatttt ccttaatggc
      781 atcttcaact tcccttctca atggacctga gttgcctatg catgtggtgc atccgtatcc
      841 cacaactcca aatcccagtc tttccagata ctccaaaagg ccggactgtc tcaaatatgc
      901 ggtaacaacc ttcgatcctg gtgcaaggga cgtcttaaca tgcggaggaa cctcaagacc
      961 aagctcgcag gccttcttcg caagtaatcc cgcaccaatc aaaacaaacg gatttgatgt
     1021 gtttgtacag cttgtaattg cggcaatcac caccgaacca ttctttatac ttgatctatc
     1081 accatccata acaatattct ctgacaaacc aaaaccacga taaccttttt cccttgtaat
     1141 agattgatcg aactcctcct tcatcaggga caagggtatg atatcctggg gtcttttggg
     1201 gccagcaagg cacgcctcca cctctcccag atcaatttct acaatttctg agtattcagg
     1261 caccgaaccc gaatcacgaa acatcagatt ctccctacag tacctctcta ccaattcaat
     1321 atgctctttt tttctgccgg tcagtttcaa atagttcaag gtttcctcat caaccggaaa
     1381 aaatccacag gtagctccgt attctggagc catgttgctg acagttgccc tgtctgcgag
     1441 tgagagtgtt gacactccct ctccaaaata ctcaacaaac ttgccaacca ccccatgctt
     1501 ccttaagaca tgcgtcacca acaaggccag atccgttgca gtggctccct ccttcattct
     1561 tcctgcaagt tttacaccca ccacatccgg cactgggaaa tatgaagctt tgcccaacat
     1621 accggcttcc gcctcaatac ctcccactcc ccaacccagg atgccaaggc cgtttatcat
     1681 ggttgtatgg gagtcagtac ctacaagcgt atcagggtaa gcaagaattt cgtcttcgtc
     1741 ctcatccaca aaaacaactt gggcaagata ctcaagattt acctgatgca caatgcctgt
     1801 agctggagga accactctga aatttgaaaa agctttctgt gcccaactta aaaatctgta
     1861 tctttcttcg ttcctttcaa actctatatc catgttaaaa ctaagcgcat caccagacgc
     1921 atacttgtcc acctgcacag aatggtcgat taccaaatcc gccttcctgt ttggatttat
     1981 acacttcgga tcacctccaa tgtccgctat cgcctttctc atggatgcaa aatccaccat
     2041 tgcaggaact cctgtgaaat cctgcaacag cactctcgca ggcttgaatg gaacatcaat
     2101 tctttcatca aacttgtcgg tattccaatt tacaaggttt aagacatgtt cttcattgat
     2161 ggaccaaccg tcatactgtc tcaatacgga ttccagcaac acccttattg aatacggcag
     2221 ccttttcaca tttatgtttt tattctcttc aagggctttt aaactgtagt agttgtatct
     2281 ttttccacct gtttcaaaat acctttttac gttaaataca tttctgtcca ttccttcacc
     2341 ccgtttgata tctatatttc aatatattat tctcatacca attggaaatt atgatagcag
     2401 aaacataaca caaagaaaag aggaggtgca aaaagcacct cctatgaatc aaaaacatca
     2461 atccaacact gatcttcctt gatatccttt acaaaaatcc tgttctcatc ttcaggggat
     2521 ttcgcctgat ggaatttaaa aagcatttta ttttctttca acaatcccat aacctctatt
     2581 tttcctcttt cggtagacat gataaacctg aagcatttgc cctgtccgtt aagctttttc
     2641 cttgcttcct ttattattct ttccgccttc aacaatggca cttgaaactg attcttcaca
     2701 ccttttactg gtcggcattg gaaaacataa tatgggacaa caccaatcct tgtcaggttg
     2761 ttagttagtt ttgcaagagt ttctccatca tcattaatgc ctttcaaaag aactgtttga
     2821 ttctttatga taattccacg atctagcagg catcttattg ccctttttga ctcttttgtt
     2881 atctccctcg aatgattgaa atgagtaaca acataaattg ttttctttct tctatacttg
     2941 tccagtatgt caagtagttc catatcatca taaattctag aagggagcac cacaggcgtt
     3001 cttgttccga agcgtattat atcaagatga tcaatatcac acagggcttc caaatattcc
     3061 tcaatctttt tgttgttgtt caaaaaagca tctccaccgg atataagtac attattgatt
     3121 tccttatgtt cccttatgta attgatgatg ttgtcaaact ccttcgttat ttcatcacca
     3181 ttaacaccaa caagcctttt tctaaaacaa tgcctgcagt acatggcgca cctgttggtt
     3241 gacaaaacga gtgcggtttg cctgtatttg tgctgcaagc ccgtcataac cgtattgtcc
     3301 ttttcaccgc tcgtatcaaa acttccgctt aagtcggtct cattgataga gggcacacac
     3361 attcttctaa tcacatcatc ttcgtcctcg ggattaatta gagacagata atactgtgat
     3421 acggacatgg gaaacttatc aataatttca ttgtacttat gtaaatcttt ttttgaaacc
     3481 tcaatattca aacattgaaa aacttcctca gccgtagtta cattattcct cagttctctt
     3541 ctccaatcca tttatcaaac ctccaatctt gataataaaa atagtatacc ctacatgcac
     3601 ttcaattgca aattccatgc caattttgtg aagtttgtgt gacaatattt aacacaatta
     3661 ccagcatcat gatatacaat caatatatgt caagacggaa gttctgtaat aatcaagtat
     3721 ttataattag tccaagcaat ctgacagtgc ttgcaaatat gaaacaaaca ccaattccaa
     3781 ccaaggtagg tattgcaaaa gacaacactg tccactttat gctctttgtt tccttcctta
     3841 tggtaagaca tgtggtggaa caaggccagt gaaacaaagt aaaaagcatc atgcttatgg
     3901 ctgttgtcaa agtccatccg tttgcaacca aaagctcctt aagctcactc aaagaaccaa
     3961 gttcaatcag acttccctgg gccatatacg ccataattat aatcggaaca actatttcat
     4021 ttgctggaaa tcctagtatg aatgccagaa ctataacccc atccagtcct agaagcttgc
     4081 caaacgggtc aagaaaatct gcgcttattc ctaaaagagt ctcacctccc acatgaatat
     4141 ttgccatcat ccaaataatt gcccctgctg gagccgcaac gataaccgct cttcccagta
     4201 caaacaaagt tctgtcgaat atggacctta ctatcacttt cagaacctgt ggtcttctgt
     4261 atggcggcag ttctagcgtg aaagaagaag gaactccttt cagaattgtt ttggacaata
     4321 aaccagatat taaaaaagtc atgactacgc ccattacaat aaccagtgta agaattaaac
     4381 tcgagaccaa agacccggtc agacctgtca aatacccggt aaaaaacatg gttatgacag
     4441 caatcagtgt aggaaacctg ccattgcaag gtacgaaatt gttggtaatt attgcaatca
     4501 gacgttctct tggcgaatca attattctac atcccgttac accaaccgca ttacatccaa
     4561 atcccataca catggtaaga gcctgtttgc cgcatgagga tgctttcttg aagtatttgt
     4621 ccaggttgaa cgccaccctc ggcagatacc ccaaatcctc tagaatcgta aagagaggaa
     4681 agaatattgc catgggaggc agcataacag acacgaccca tgcaagaacc ctgtatactc
     4741 caagcaccaa aattccatgt agccaatttg gcgcattaac caattcaaga aaatatgtta
     4801 ttctttcttc caagaaaaag aaaaatcttg ccagcattgc agacggataa tttgcccctc
     4861 ttatggtaat ccagaaaacc acaaaaagaa gaaccatcat taaaggatac cccaaatatt
     4921 tccccgtaat gattctatct atttttctat ccctttctac gtacttttca tcctcgt
//
LOCUS       DAONVY010000114         4896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12495_length_4896_cov_3.655237, whole genome shotgun sequence.
ACCESSION   DAONVY010000114 DAONVY010000000
VERSION     DAONVY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12495_length_4896_cov_3.655237"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..360)
                     /locus_tag="PLI11_04205"
     CDS             complement(34..360)
                     /locus_tag="PLI11_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52098.1"
                     /translation="MMFANLTEAEKEFYNSGEFGKLLEKFTVRKSWARQSRKYEKDKK
                     YAGYWDWLDEWPQAYGRSFKGVKEQMSVSIAATAMTHKGRSYVKGNVDYGTGLEDFDF
                     SLGKAQ"
     gene            complement(438..1313)
                     /locus_tag="PLI11_04210"
     CDS             complement(438..1313)
                     /locus_tag="PLI11_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52099.1"
                     /translation="MALFIYLGGNYEEIKHELYLDEEHEYRIVNTFNKISLYISEKLV
                     AYVDYTYDEDITVRDSFGNVVGERVRNDRTPQFGFWKITVENLKGYIKDLEYVNSVVT
                     QQDFPERDKRLINYETWVATDDLNRTTPTNVEAGNRKKNKYVGLFYFLNHADEREHVI
                     HDVTKIFMEQGLDELKTILPNTLRIYWAEPYFGYYLNTDTWVYRKHAYMFNAAGIDFI
                     FIDFSNNIVYEEGLQALLETWLRIRKEGGDTPQVVFMTGDMPHVLVHDLYVLKDLVFN
                     NPEYDELWLNGTENP"
     gene            complement(1354..2193)
                     /locus_tag="PLI11_04215"
     CDS             complement(1354..2193)
                     /locus_tag="PLI11_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPZ52100.1"
                     /translation="MILSTPTDYLSYRLGLEKAIRLLCETGYDALDFTMFCLANEKDP
                     LHTEGNKYVEKIKKIVDGYGVYFNQAHAPFPSFKEGDEEFNKRMFKAIVRSMEICCIL
                     EIGIVVVHPIDWSNAQEVMQKNVEFFNSLIPYCEKYNVKIALENNHLYQTAEQLSELV
                     DSLDDKFFTVCLDTGHCHLFGQDTASMIRKLGNDRLKALHVHDNDAVIDRHTAPYFGT
                     MDWTSITNALAEINYSGDFTLEADSFYHKLPNELLPASAKYLHDIGRLLVEMVEKAKK
                     SNS"
     gene            complement(2262..3104)
                     /locus_tag="PLI11_04220"
     CDS             complement(2262..3104)
                     /locus_tag="PLI11_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPZ52101.1"
                     /translation="MKKFQEYIICIFGAALVATGVYFFKMPNNFSTGGVTGISVVLSG
                     FFKLQSASIIASALNILMLILGFLVLGKQCGLKIVLGSISMSATLLLLEFFIPMRQPL
                     TDEPFLELIFAVLLPGVGSAILFNINGSTGGTDIIAMILRKFTSINIGLALFLSDLII
                     TLGAFLFGVKVGLFSLLGLLSKTMIVDTVIESINTVKCFSIITLNPGPISEFITTKLR
                     RSTTVIDAVGGYTNENKKVVVTVMNRMQAIQLRRYLKEHYPEAFIIITNSSEIIGKGF
                     RGVN"
     gene            complement(3637..>4896)
                     /locus_tag="PLI11_04225"
     CDS             complement(3637..>4896)
                     /locus_tag="PLI11_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HPZ52102.1"
                     /translation="QTEMASFAYLAADEEVEFAITPNKDFSEVVIRPLSKKVEFDVQN
                     GVIRFRIKKAGQYTVELDGHHNALHLFVNPIEEYLKDDNTIYFGPGVHYAGHIDMQDN
                     QTIFVDSGAVVHCSITAADKKNIAIKGHGIIDNSSFKRPSKSCLRCPTSVVFTRCMNV
                     EISGVIFNDASAWTATFFNCDNILVDNIKTIGMWRYNSDGVDFVNSSNGIVRNSFLRN
                     FDDVIVLKGIKGYDHRNVENILVENCVLWCDWGRALEIGAETCADEYRNIVFRNCDII
                     HASTKAMDLQNGDRANVHMVLFEDIRVEYSKYAMPEVYQESDDMEYVWDGKPNIPWLF
                     YAHLYCGLWSDDNLLGTNSNIRLKNIQVFMDEGLERPKIGLYGANEDHMTYDITIEDL
                     YINGVKVVSYEDANIHTNEFVQNVDVK"
BASE COUNT         1440 a         1113 c          747 g         1596 t
ORIGIN      
        1 ggcttcttgg aattgttcct cgaaatgaag gccctactgt gctttaccca agctaaaatc
       61 aaagtcctct agaccggtcc catagtcaac attgccttta acataacttc ttcccttatg
      121 ggtcattgct gttgctgcta tggaaacgct catctgctct tttacacctt tgaaacttct
      181 accatatgct tgtggccact catccagcca atcccagtat cctgcatact tcttatcctt
      241 ttcatatttc cttgattgcc ttgcccagct ctttctgaca gtaaacttct caagaagttt
      301 tccaaactct ccactgttgt agaactcctt ttctgcttca gtcagatttg cgaacatcat
      361 ttctttcgta tatgttccaa cagtttattc tgaaattgtc catacatctc catcaggatc
      421 atcattgttg cctaaaatca agggttttcc gtcccattta accacaactc atcatattcc
      481 ggattgttga atacaaggtc tttcaacaca tacaagtcat gtaccaatac atgaggcata
      541 tcccctgtca taaacacaac ttgaggagtg tctccacctt cttttcgaat tctaagccag
      601 gtttcaagca aggcttgaag cccttcttca taaacaatat tgtttgaaaa atcgataaat
      661 atgaaatcaa taccagcagc gttaaacata tacgcatgct ttctatacac ccaagtgtca
      721 gtattcagat aatagccgaa atatggttcc gcccagtata ttctcaaagt attcggcaat
      781 attgttttca gttcatcaag accctgctcc ataaatattt ttgtgacatc atgaattaca
      841 tgttcacgct cgtctgcatg attcaggaaa tagaacagac caacatactt gttcttcttt
      901 ctgttacctg cttctacgtt ggttggtgtg gtcctgttca gatcatccgt tgccacccat
      961 gtttcgtaat tgattagtct tttgtccctt tcaggaaaat cctgctgtgt aacaacactg
     1021 ttcacatact ctaaatcttt gatataacct tttagattct ccactgttat cttccagaag
     1081 ccaaactgag gtgtcctgtc atttcttacc cgttcaccta caacatttcc gaatgaatct
     1141 ctgacagtta tatcttcgtc atatgtataa tccacatatg ctaccaactt ctcacttata
     1201 taaagactta tcttgttgaa agtatttaca atcctgtatt catgttcttc atcaagataa
     1261 agttcatgct taatttcctc ataattccct cctaaatata tgaaaagagc cacaacctat
     1321 attatcgagc agttgcagct ctgtcttact ttactagcta ttactctttt tagctttttc
     1381 gaccatttca actagcagcc ttccaatatc atgcagatat tttgcactcg caggaagaag
     1441 ttcgttagga agtttatgat aaaaactgtc agcctcaagc gtaaaatcac cggagtaatt
     1501 gatttctgca agagcgttgg taatagaggt ccaatccata gttccaaaat atggagccgt
     1561 atggcggtca atgacagcat cattgtcatg aacatgaaga gctttcaatc tgtcattccc
     1621 cagttttctt atcatgcttg ctgtatcctg cccaaacaaa tgacagtgtc ctgtatcaag
     1681 acatactgta aagaatttgt catcaaggga atctacaagt tccgataact gctctgccgt
     1741 ttgataaagg tggttgtttt ccaatgcaat cttcacattg tatttctcac agtatggtat
     1801 gaggctgttg aagaactcta cgttcttctg catcacttct tgtgcgtttg accaatctat
     1861 gggatgaaca acaacaattc caatttccag tatgcagcat atttccatcg atctaacaat
     1921 tgctttaaac atccttttat tgaactcctc gtccccttct ttaaaagatg gaaacggggc
     1981 atgggcctgg ttgaaataaa cgccataccc atctacaatc tttttaattt tttcaacata
     2041 cttgttacct tcagtatgca gcggatcctt ttcatttgca agacagaaca ttgtgaagtc
     2101 tagtgcatca tatccagttt cgcataatag cctgattgcc ttttccaaac ccaaacggta
     2161 tgacagataa tccgttggtg ttgacaaaat catttatctc ttcctttcta aataaaaata
     2221 ccctatacta tcatttttag aatcagtttt caatatggtt atcagtttac tcctctgaaa
     2281 ccttttccga taatttcact ggaatttgtt ataattatga atgcttctgg ataatgttct
     2341 tttagatatc ttctcagctg tattgcctgc attctgttca tcacggtaac aacaaccttt
     2401 ttgttctcat tcgtatatcc tcctacagca tcaataacgg tagtgcttct cctcagtttg
     2461 gtggttataa attcgctgat cgggccagga ttcaatgtaa taattgaaaa gcattttact
     2521 gtattaatac tttcaataac ggtatcaact atcatggtct ttgataataa ccccaataac
     2581 gagaacaaac caaccttcac cccgaataaa aatgcaccca gagtaattat caagtctgat
     2641 aagaacagtg caagaccaat gttgatactt gtgaactttc ttaatatcat tgcaattata
     2701 tcagttccac cagtgcttcc atttatatta aacagaatag cagaacccac accaggtaaa
     2761 agtacagcaa aaatcagttc aagaaagggt tcatcagtta atggctgcct cattgggata
     2821 aaaaattcga gcagtaacaa tgtggcagac atcgagattg agccaagtac tattttcaaa
     2881 ccgcattgct ttcccaaaac taaaaaacca aggatcaaca tcaaaatatt caatgcagat
     2941 gcaattattg aagctgattg gagtttgaaa aatccgctta aaacaacgga gatgccagta
     3001 acccctccag ttgagaagtt gtttggcatt ttgaaaaaat aaacaccagt tgcaacaagt
     3061 gcagctccaa atatgcaaat tatatactct tgaaacttct tcatacaaac ctctttcctt
     3121 gcttcaataa tataaacaac ataactatta tacaaatcaa aacaaattat tgtataattt
     3181 ccatataata tttttactct tgtcccgacc aatggctatc agattgtcat ccctaaaaac
     3241 agtttaattg ttccggcatt acagttgatg ctttttcata ttccttaaac ctgcttgact
     3301 aaaaggtttg aactggaaat gttgttggaa tcagggtcgt ttgcactctc ctgcttgctc
     3361 cacaatcctt caacatcgaa tatatatgtt gtcacttgct ctggatttat caacatatat
     3421 ccttttgtct gccccttatc gcttaaggta acagcatcaa tctcaacctg acttatctat
     3481 taactacatc catgcagtaa gatactggca caataatgta gaaagcaaga cttatgcaag
     3541 cttctctgta ttgaggccaa acaatatcga gataacactt gcagcaagtc ttcacgcaat
     3601 actaattctt ctgttttacg aaaacgagtc tgatgattat ttgacatcaa cattttgcac
     3661 aaactcgttg gtgtgaatgt tcgcatcctc ataggataca actttcacac catttatata
     3721 tagatcttca atagttatat catatgtcat gtgatcttca tttgcaccat aaagcccaat
     3781 tttaggtctt tcaagacctt catccatgaa tacttgaata ttcttaagcc ttatattgct
     3841 gtttgtgcca agaagattgt cgtcagacca aagaccacag tacaagtgag catagaaaag
     3901 ccatggaatg tttggctttc catcccatac atactccata tcatcactct cctgataaac
     3961 ctctggcatt gcgtatttgc tgtactccac tctaatgtcc tcgaaaagta ccatgtgcac
     4021 atttgctctg tctccatttt gaagatccat cgcttttgtt gaagcatgta tgatatcaca
     4081 gtttctaaag acaatatttc tgtactcatc agcacaggtt tcagccccaa tttcaagagc
     4141 tcttccccag tcgcaccaca agacacaatt ctctaccagg atattttcaa cattcctatg
     4201 gtcataacct ttaatcccct tcaaaacaat gacatcatca aagttgcgga ggaagctatt
     4261 cctgacaatt ccattggaac tgttgacaaa gtcaacacca tccgaattgt atctccacat
     4321 accaattgtt ttgatattgt ccaccaagat attgtcacag ttaaagaacg tagccgtcca
     4381 tgcgctggca tcattgaata tgactccact aatttctaca ttcatacatc tggtaaatac
     4441 aacacttgtt ggacatctta gacacgactt ggaaggcctt ttaaatgagc tgttgtcaat
     4501 tattccatgc ccttttatag ctatgttctt tttatcggca gcagttatag agcaatgtac
     4561 taccgcaccg gaatcaacaa aaatcgtttg attgtcctgc atatctatat gcccggcata
     4621 atgcacccca gggccaaagt atatggtgtt atcatcttta agatactctt caatcggatt
     4681 tacaaacaaa tgaagagcat tgtggtgtcc atccaattcc actgtatact gccctgcctt
     4741 ctttattctg aaccttatta caccattttg tacatcaaac tctacttttt tagacaaagg
     4801 tcttatgact acttcactga agtccttgtt tggagtgatg gcaaactcca cctcttcatc
     4861 ggctgccaaa tatgcaaagg acgccatttc ggtttg
//
LOCUS       DAONVY010000115         4887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12520_length_4887_cov_2.861548, whole genome shotgun sequence.
ACCESSION   DAONVY010000115 DAONVY010000000
VERSION     DAONVY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12520_length_4887_cov_2.861548"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLI11_04230"
     CDS             <1..398
                     /locus_tag="PLI11_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52103.1"
                     /translation="AEFLKVIPYTDTFYYDIKGDRYVYDNYVFGDYDLVISNLAKLIT
                     KGYDVRIRIPLIPQVNDSVEVMRTVYTTLKNLGVTKVSILPFHRLGSGKYDALNQEYG
                     FKNTKELSKKKIVEIVDIFESIEVEIENH"
     gene            complement(566..1195)
                     /gene="upp"
                     /locus_tag="PLI11_04235"
     CDS             complement(566..1195)
                     /gene="upp"
                     /locus_tag="PLI11_04235"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020061432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPZ52104.1"
                     /translation="MGSVHGIDHPLIQHKVAMLRDVNTQTKDFRELVSEIAMLMCYEV
                     TRNLPLKEVEVETPLTKCKTKMLAGKDVCFVPILRAGLGMVDGVLRLMPFAKVGHIGL
                     YRDPETLQPVEYYCKLPKDVADRMVIVLDPMLATGGSASSAITFIKRRGAESIKLMSL
                     IAAPEGIERVQQDHPDVDIYCAAIDEKLNEHAYIVPGLGDAGDRLFGTK"
     gene            complement(1222..1707)
                     /locus_tag="PLI11_04240"
     CDS             complement(1222..1707)
                     /locus_tag="PLI11_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HPZ52105.1"
                     /translation="MKILFVCTGNTCRSYMAETLLKEYIRKAEESDLIPQNAIKVESA
                     GLFVVEDTANKKAISVMEEEFGINTETHSARQITRKLIEDSDLILTMTNGHKRAILDT
                     YPVYKGKIYTLLEYAFPEATVVDIQDPYRGDRQDYIESAVSINNAIKSMFYKLIEELE
                     K"
     gene            complement(1724..2773)
                     /locus_tag="PLI11_04245"
     CDS             complement(1724..2773)
                     /locus_tag="PLI11_04245"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPZ52106.1"
                     /translation="MKTEIIKVDPENIDEKIMKYCGEKIREGKLVCFPTETVYGLGAN
                     ALDEKAIEGIFTAKGRPQDNPLIVHIAGYDMLDDIVKTTQREEILLRKLSDRFWPGPL
                     TLIVTKDESMSDVVTSGLNTVGVRYPSNSIAQTLINYANVPIAAPSANISGKPSPTKA
                     SHVIQDLTGKVDIIIDGGPCQVGLESTVLDIVSWPPIILRPGAVTQEDISEVVIEVSE
                     HGWQKEVDKPRSPGMKYKHYAPNAKITIYKGEESKVASRIKEELKNANNLGLNAFVLA
                     SEETLCYYNKEKALSLGSRYNPLEQASRLYDCLRKFDELKADVVFAEAIENKGVGDAI
                     MNRLYRAASGRLISI"
     gene            complement(2777..3847)
                     /gene="prfA"
                     /locus_tag="PLI11_04250"
     CDS             complement(2777..3847)
                     /gene="prfA"
                     /locus_tag="PLI11_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPZ52107.1"
                     /translation="MLEKLQATEDRYEEINQKLADPDILSDQEAYLKVVKEHAELEEL
                     VSVFREYKKCVQNRDEAKELLTQNLDGEFKGLVQLEYDEMSIKAEELESKLKILLTPK
                     DPNDNKNVIVEIRGGAGGEEAALFSAVLFRMYTRYAERMGWHSEIIDFNETGIGGFKE
                     VVFSIEGKGAYSRLKFESGVHRVQRVPTTEASGRIHTSTATVAVLPEIDDVEIEINPN
                     DLEIDTFRSSGAGGQHVNKTESAIRITHKPTGIVVSCQDEKSQLKNREKAMKVLRSKL
                     YELEMEKQEKELAQKRRSQVGTGDRSERIRTYNYPQSRVTDHRIDLTLYKLDDILDGD
                     LDELIDALITSEQAEILSGGID"
     gene            complement(3865..4461)
                     /gene="recR"
                     /locus_tag="PLI11_04255"
     CDS             complement(3865..4461)
                     /gene="recR"
                     /locus_tag="PLI11_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPZ52108.1"
                     /translation="MALYTSSVARMIEEFEKLPGIGHKSAQRLAFYLLGQSKEKVAAF
                     ANAILEARNQLQYCSVCMNMSDKEVCDICSNPVRDTGIICVVEDPRDVVAMERSREFK
                     GLYHVLHGVISPMSGVTVDDLKIKELLARINDNTKEIIVATNPNIEGEATAMYLARLI
                     KPLNVSVTRIAHGIPVGGDLEYADEVTLAKALQGRREM"
     gene            complement(4475..>4887)
                     /locus_tag="PLI11_04260"
     CDS             complement(4475..>4887)
                     /locus_tag="PLI11_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPZ52109.1"
                     /translation="KIAEIFYENVRNAFIFYGGFFMAKGMRGGFGGGAKKGGAGNMQK
                     MLQQAQRIQAQLQQEMEVLSEETFEITSGGGAVKVFISGDKIIQDIEIMPEIVDPEDI
                     EMLQDTIVAAVNEALSLVDEKQREIQEAYTGGLM"
BASE COUNT         1299 a         1176 c          819 g         1593 t
ORIGIN      
        1 ttgctgaatt tctgaaggtt ataccctata ccgatacctt ttactatgac ataaagggag
       61 acaggtatgt ttatgataat tatgtgtttg gtgattatga tttagtgata agtaacctgg
      121 caaagctgat tacaaagggc tatgatgtga gaataaggat ccctctgatc ccgcaggtga
      181 atgatagtgt tgaagtaatg agaaccgtat atacaaccct taaaaatctg ggtgttacaa
      241 aggtttcgat actgcccttc cacagactcg gaagcggcaa gtatgatgcc ttaaaccagg
      301 aatacggatt taaaaataca aaggagctca gtaaaaagaa aatagtggaa atagtggata
      361 tatttgaaag tattgaagtt gaaatagaga atcattaata aaaagcggat tagttagatt
      421 aaactgacga gacggatata gtggtaaacc ccagctcgat ttatggctta tgtctgaaga
      481 aaaagacaga acaaacggca ccctgccatg ttgataagga aagtaaaata aatgcaaaat
      541 gataccggtt tcccggtatc atattttatt tcgtaccaaa caatctgtct ccagcatccc
      601 caagacctgg cacaatatat gcatgttcat tcagcttctc gtctattgct gcacaataga
      661 tatcaacatc agggtgatcc tgttgaaccc tctcgatacc ctccggagct gcaatcagag
      721 acataagctt gattgattca gcgcctcttc tcttaataaa tgtaatggcg cttgatgcag
      781 aacctcctgt tgcaagcatt ggatcaagaa ctataaccat tctatcagcc acgtcttttg
      841 gcagcttgca atagtattca acaggctgca aggtttccgg atccctgtaa agtcctatgt
      901 gacctacctt tgcaaaaggc attagcctca aaacaccatc caccattccc agacctgctc
      961 taagtatggg tacaaaacat acatctttgc ccgcaagcat ctttgtcttg catttcgtta
     1021 atggtgtctc tacttccact tctttaaggg gcagattacg agtaacttcg taacacatca
     1081 gcatcgcaat ttccgatacc aattccctga aatccttagt ttgagtattc acatctctta
     1141 acatcgcaac cttatgctga attagtggat gatcaatacc gtgtacactt cccatattta
     1201 tcctccataa ctttagattt gctacttctc cagttcttca atcaatttgt aaaacatgga
     1261 tttgatagca ttgtttatag acacagcact ttcaatatag tcctgtctgt ctcctctgta
     1321 agggtcctga atatcaacaa cagttgcttc aggaaaagca tattcaagca gcgtatatat
     1381 tttacccttg tatacagggt atgtatcaag aattgcccgc ttgtgtccgt tagtcatagt
     1441 gagtatcaag tcactgtctt caataagctt tcgtgttatt tgcctggcac tatgcgtctc
     1501 ggtatttata ccaaactctt cctccataac actgatagcc tttttattgg cagtgtcctc
     1561 caccacaaac aaacctgcag actccacctt tatggcgttt tggggtataa ggtcgctttc
     1621 ttctgccttt cttatatact cctttagtaa tgtttcagcc atgtagcttc tgcaggtatt
     1681 tccggtacag acaaacaaaa tcttcaaaat caaacctctt tctctatata ctaatcaacc
     1741 tgccactggc ggctctatac aacctgttca taatggcatc gcccacgcct ttgttttcaa
     1801 ttgcttcggc aaaaaccaca tctgctttaa gttcatcaaa ttttctcaaa cagtcataaa
     1861 gccttgaagc ttgttccagt ggattgtacc tacttcccaa ggacagtgct ttttctttat
     1921 tataataaca aagggtttct tcagatgcaa gaacaaatgc attcaaaccc aaattatttg
     1981 cattctttaa ctcctcttta atccggcttg ctaccttgct ttcctcacct ttataaattg
     2041 ttatttttgc attaggagca tagtgtttgt atttcattcc cggagacctt ggcttgtcca
     2101 cttccttctg ccatccatgc tcggaaacct ctataacaac ctcgctgata tcctcttgag
     2161 taacagcacc aggtctgagt atgattggag gccaggatac aatatccaaa accgttgact
     2221 caagaccaac ctgacatggc cctccatcaa taataatatc caccttgccg gtcaaatcct
     2281 gaataacatg acttgccttt gtgggacttg gtttccccga tatatttgca ctgggtgcag
     2341 cgatgggaac attagcgtaa tttataaggg tttgtgcaat actgtttgag ggatatctga
     2401 ctcctacagt atttagtcca ctcgttacca catcggacat ggactcatct tttgtaacaa
     2461 tcaatgtcaa gggacctggc caaaacctat cgcttaattt tcttaaaagg atttcttccc
     2521 tttgggttgt tttcacaata tcatcgagca tgtcatagcc tgcaatgtga actataagtg
     2581 ggttgtcctg aggtcttccc ttagctgtaa atattccttc aatagctttt tcatccaaag
     2641 cattagctcc aagcccatag acagtttcag tggggaaaca gacaagtttt ccttccctta
     2701 tcttttcccc acagtatttc attattttct catctatatt ctcaggatca actttaatta
     2761 tttctgtttt cacttcttag tcgattcctc ccgacagtat ctccgcctgt tcacttgtaa
     2821 tcaaagcgtc aataagctca tctagatctc catccaatat gtcatccaat ttatacaaag
     2881 tcaaatcaat acgatggtcc gttaccctgc tttgaggata gttgtacgtc cttattctct
     2941 cacttctgtc tcctgttcct acctggcttc ttcttttttg tgcaagttct ttttcctgtt
     3001 tctccatctc aagctcataa agcttcgagc gtaatacctt cattgccttt tccctattct
     3061 taagctgtga cttttcatcc tggcaggata caacaattcc agtgggcttg tgtgttattc
     3121 ttatggcgga ctctgttttg ttaacatgct gtcctcctgc accactggat ctgaaagtat
     3181 ctatttccaa gtcatttggg tttatctcaa tttccacatc atctatttca ggcaaaacgg
     3241 caacagtagc ggtggatgtg tgtattcttc cacttgcttc cgttgttgga actctctgaa
     3301 ccctatgcac gccgctttca aatttgagtc tgctgtacgc tccttttcct tcaatggaaa
     3361 aaacgacttc cttgaatcca cctattccgg tttcattgaa gtcaattatt tcagaatgcc
     3421 agcccatgcg ctcggcatat cttgtgtaca ttctaaacaa aacggctgaa aacaaggcag
     3481 cttcctctcc acctgcgccg cctcgaattt ctacaataac gtttttgttg tcattaggat
     3541 cctttggagt aagcagaatc ttgagctttg attcaagttc ctccgcctta atcgacattt
     3601 catcgtattc cagctggaca agccccttga actcgccatc aagattctga gtcaaaagtt
     3661 cttttgcctc atccctgttc tggacacact ttttatactc cctaaataca gaaacaagct
     3721 cttccagttc cgcatgttcc ttgaccacct tcaaatatgc ttcctggtct gaaagaatat
     3781 ccggatccgc gagcttttgg tttatttctt catatctgtc ttccgttgct tgaagctttt
     3841 cgagcatata aacttccttt caaattacat ttctctcctg ccttgcaggg cttttgccaa
     3901 tgttacctcg tcggcatatt ccaaatcgcc accaacaggt attccgtggg cgatacgtgt
     3961 tacgcttaca ttaagaggct taataagtct tgccagatac atggccgtcg cctctccttc
     4021 gatattcgga ttggtggcaa ctattatctc tttagtattg tcatttattc ttgccaaaag
     4081 ttcctttatt ttcaaatcat caacagtgac accactcatt ggtgatatga ctccatgaag
     4141 cacatgatac agacctttga attctcttga tctttccatt gcaacgacat ctctgggatc
     4201 ttcaaccaca catattattc cggtatccct tacaggattg gagcatatgt cgcaaacctc
     4261 cttgtcggac atattcatgc agacactaca gtactgcagc tggtttcttg cctcaagaat
     4321 cgcatttgca aacgccgcaa ctttttcttt gctttgacct aataaataaa aagcaagcct
     4381 ttgagccgac ttgtgtccaa tcccaggcaa cttttcgaac tcttcaatca ttcttgcaac
     4441 agaacttgtg tatagagcca tttctttctc ctgtctacat cagtccgcct gtataggcct
     4501 cttgaatttc cctttgtttt tcgtcaacca acgacaatgc ttcattaact gcagcaacaa
     4561 tagtatcctg cagcatctct atatcctcag gatcaacaat ctcaggcatt atttcaatat
     4621 cctgaataat cttgtcacca gatatgaaaa cctttactgc gccaccacca gatgttattt
     4681 caaaagtttc ttcgcttaat acttccattt cttgttgaag ctgtgcttga attctctggg
     4741 cctgctgaag cattttttgc atgttgccag ctccaccttt ttttgcccca ccgccaaaac
     4801 cacctctcat gcctttcgcc ataaaaaacc ctccataaaa aataaatgca tttcttacat
     4861 tttcataaaa aatttctgca atttttt
//
LOCUS       DAONVY010000116         4862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12601_length_4862_cov_3.262534, whole genome shotgun sequence.
ACCESSION   DAONVY010000116 DAONVY010000000
VERSION     DAONVY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12601_length_4862_cov_3.262534"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLI11_04265"
     CDS             <1..1088
                     /locus_tag="PLI11_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPZ52110.1"
                     /translation="ALLEALDGEQNSTFRDHYLELPYDLSRVLFIATANNLDTIPPAL
                     LDRMEIIEVPSYTEDEKFEIARRHLIPKQLAEHGLTSKKVYISDEILRSIITEYTAES
                     GVRELERNIAAVLRKSVKIMTEDKTRKITIDEKLLVEMLGASSRKRDKISDKDEVGIA
                     NGLAWTAFGGVTMAVEVNILPGSGNIELTGRLGDVMKESARIAISYIRSRADKLGIDK
                     DFYKNNDVHIHVPEGATPKDGPSAGITMATAIISALTGIPVKSTVAMTGEITLRGKVL
                     PIGGLREKTLAALRSGVKTVIIPDKNRNMEPELPEAVRNELEIVYVSHMDEVLDVALS
                     SSIREKVRKITVDSYNTFSDAKYAIQN"
     gene            1191..2027
                     /locus_tag="PLI11_04270"
     CDS             1191..2027
                     /locus_tag="PLI11_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPZ52111.1"
                     /translation="MYQLQTRTTQQMMSYVIITRENRCIVIDGGNAGDADYLLEFLKD
                     KTNSEKVNIDAWFFTHVHSDHVNAFVTLVNSKMDEFFVNKVLYNFPDRELVSQYDYTD
                     STVLTMDEFYKAVEKLSDTEVVILQNGDKFSFGDVHFEVLLVYDESFMRNSNVVNNSC
                     SIIRMEVEGQTVLFLGDAGVEQGKVLLEKHGEELRSDFVQMAHHGQNGVTKEVYEAIK
                     PKACLWPTPLWLYDNDIGKGYGSGPWQTLVVRDWMQELGVKHHFIDKDGTHEIVLPFV
                     FD"
     gene            complement(2024..3442)
                     /locus_tag="PLI11_04275"
     CDS             complement(2024..3442)
                     /locus_tag="PLI11_04275"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPZ52112.1"
                     /translation="MIKRRSSFVTVIDNTWKGAIGFTVFIILELFENGVSSNLKYVVL
                     VLALVIAPILFLLQFIRWRITYFIYDGDTVTLEKAGIFKKKLTIPVDKISTVDIKKNI
                     LGHLTGTSRVKVDSGGKQMGNDNSGAEINLIFDDEFARQIKAYIFKEEVEVHTKGFRM
                     SGMELFIDGLLRRKLIYAIPFFFSIYVLATAVLNEKTVDSITSKALTYIKSLEFFVIL
                     ILAVIFIIITNITSIVKELIKYHNFTLQKQRDVIATSFGLFTTQNYSLPIEKIRAVIL
                     RQNTLFRLLGYWSISVVCIGLGDEKNEKPVIFPLANKERLKSILSMIDPAIVVDDPIH
                     KVPKRGRVFYYLPLVVYLILVLIIFAVTSIIFEVKTIYFVAPALVLPLLLVVAFLSLK
                     NAGLLYNENYVTVTNGIFTKTTTIVPVRHVQSVGMREAFYKRKKNLCNLHLKFYPGFT
                     NPSLSSFDKVHFAAIAEMLEKG"
     gene            complement(3423..3926)
                     /locus_tag="PLI11_04280"
     CDS             complement(3423..3926)
                     /locus_tag="PLI11_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPZ52113.1"
                     /translation="MQKMQHPDKKVMKVWRFKYYVLLALQIIAYVIALVFALTSGNKD
                     FAIFATTVFVLTAAAVIAAATSLPKLSYKRRKYLFTEDKIEIVKGIIFIETSMIPISR
                     IQHLTIDEGPLLRRHNLASIVVYTTAGFFKMEGLAREDAYKIADFLKNTVNIKVRNED
                     ENDKEAQ"
     gene            complement(3948..4832)
                     /locus_tag="PLI11_04285"
     CDS             complement(3948..4832)
                     /locus_tag="PLI11_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPZ52114.1"
                     /translation="MKKKVTLTLAIVIAFILIILAASSLYTVKEDEYALILRFSKVES
                     IKSESGLYFRIPFIETILKFPKNLQLYDLRPSDVLTSDSKAMSVDSYVLWKISDPLQF
                     YKTLGSIAEAQVRLDASTYNALKNVIGRKEQNAIITQQDTGRDTLNEEIFQNVKNTST
                     GYGIEIMDVKVKRFDLPQANEEAVYRRMISDRNQIAARYLAEGQSEANMLKNEVDKKV
                     SLLISEARVTAEELIAEGEAEYMRLLAETFDTKEKQDFYIFMRSLEALKASLTGDDKT
                     VVLGRDSDIAKILMGISE"
BASE COUNT         1384 a          921 c         1063 g         1494 t
ORIGIN      
        1 ccgctcttct tgaagctttg gacggagagc agaacagtac attcagagac cactatctag
       61 aactgcctta tgacttgtcc agagtgctgt ttatagcgac ggcaaacaat cttgatacca
      121 tacctcctgc actgcttgac aggatggaga ttatagaggt gccaagctac actgaggacg
      181 aaaagtttga aattgcaaga aggcatttga ttccaaagca gcttgcagaa cacggactta
      241 cctcaaagaa agtttatatc agtgatgaaa tcctaagaag cataatcaca gagtatactg
      301 cagagtccgg tgtgcgtgaa ttggaaagaa acattgccgc agtgcttaga aaatcagtca
      361 agattatgac cgaggacaag accaggaaga taacaattga tgaaaagctt cttgtggaga
      421 tgcttggtgc aagttcgagg aagcgggaca agatatccga caaggatgaa gtaggcattg
      481 caaatggtct tgcatggacg gcatttggcg gcgttaccat ggcagtggaa gtaaacatat
      541 tgcccggcag tggcaacatc gaactcactg gcagacttgg agatgtaatg aaggagtctg
      601 caaggattgc aataagctac attcgttcca gagcggacaa gcttggtatt gacaaggact
      661 tctacaaaaa caatgatgtc cacattcacg ttcctgaagg agctacgcca aaggacggtc
      721 catcagcagg tataaccatg gcgaccgcca ttatatcagc cctcactggc attcctgtga
      781 aatctacagt ggccatgact ggtgagatta ctttgagggg caaggtgctt ccaattggag
      841 gactcaggga gaagacgctg gccgctttaa gaagtggtgt taagactgtc attattcccg
      901 ataagaacag gaacatggag cctgaacttc ctgaggcggt aaggaacgag ctggagattg
      961 tatatgtatc ccacatggat gaggtgctgg atgtggcatt aagttcaagt ataagagaga
     1021 aggttagaaa gattactgta gacagctaca atacatttag cgatgcaaag tatgcgattc
     1081 agaattaatt tgggcgaggg aaacctcgct ctttttttta ttgattaaga tttctgcaag
     1141 tgataatata accttgaaat ttatataggg agtggatgaa cttgacaaaa atgtatcagc
     1201 tgcaaaccag gaccacacaa cagatgatgt cttatgtgat aattacaagg gaaaacaggt
     1261 gtatagtcat tgatggtgga aatgcgggag atgccgacta cttattggag ttcctaaagg
     1321 acaagacaaa cagtgaaaaa gtaaatattg atgcatggtt ctttacccat gtgcactcgg
     1381 atcatgtgaa tgcctttgta acattggtaa actcaaaaat ggatgagttt tttgtcaaca
     1441 aggttctgta taatttcccg gacagggaat tggtttcaca atatgattat actgattcta
     1501 ctgtactgac catggatgag ttttacaagg cagtagagaa gctttccgat acagaggttg
     1561 ttattctaca gaacggtgat aagttttcat ttggagatgt gcattttgaa gtattgcttg
     1621 tttatgatga aagctttatg agaaactcaa atgtggtcaa caacagctgc tcgattatca
     1681 ggatggaagt ggaaggacag actgttttgt ttttgggaga tgcaggagtg gagcagggga
     1741 aggttctgct tgagaaacat ggtgaggaat tgagaagcga ttttgtgcaa atggctcatc
     1801 atggacaaaa tggtgttaca aaagaagtgt atgaagcaat aaagcccaag gcatgtcttt
     1861 ggccgactcc cttgtggctt tatgataacg acattggaaa gggttatggt tctggtccct
     1921 ggcagacatt ggttgtaagg gactggatgc aggaattggg tgtcaaacat cactttatag
     1981 ataaagacgg cacccatgag attgtgctcc cttttgtgtt tgattaaccc ttttccagca
     2041 tttctgcaat ggctgcaaaa tgcaccttgt caaaacttga aagactggga tttgtgaatc
     2101 ctggatagaa cttaagatgc agattgcaca aatttttctt tcttttgtaa aaagcttccc
     2161 tcatgcccac cgattgcaca tgtcttactg gaacaatggt agtggttttt gtgaagattc
     2221 cgtttgttac agttacatag ttttcattgt acaacagtcc tgcgtttttc aaggacaaga
     2281 atgcaacaac cagaagaagg ggcagaacaa gtgccggtgc gacaaagtat atggttttta
     2341 cttcgaaaat gatgctggtt accgcaaaaa tgatgagcac aagtatcagg tatactacaa
     2401 ggggcagata gtagaaaact ctgcctcttt ttggtacctt gtgaattggg tcgtcaacaa
     2461 cgattgcagg atcaatcatg gacagaatgc ttttaagtct ttccttgttg gcaagaggaa
     2521 agataactgg tttttcattc ttttcatcac ccaaaccaat gcataccacc gatatggacc
     2581 agtagccaag aagtctgaag agagtatttt gtcttagtat tactgccctt atcttttcta
     2641 ttggcagtga atagttttga gttgtgaaaa ggccaaagct agttgcaatc acgtctcttt
     2701 gcttttgcaa tgtgaagttg tgatatttta tgagttcctt tactattgaa gtgatattgg
     2761 tgataataat aaatattaca gcaagaatca aaatgacgaa aaattcaagg cttttaatat
     2821 acgttaatgc cttggatgta atgctgtcta cggttttttc atttagtact gcagttgcca
     2881 gcacgtaaat cgaaaagaag aaagggattg catagattag ttttcttcta agtagaccat
     2941 ctatgaacaa ttccatgccc gacattctga agcccttggt gtgtacttca acttcttcct
     3001 tgaagatgta tgcctttatc tgccttgcaa actcatcatc gaatatcagg ttgatttcag
     3061 cgccgctgtt gtcgttgccc atttgttttc cgccactgtc aactttcact cttgaggtac
     3121 ctgtaaggtg tccgagaata ttctttttta tatccaccgt cgatatctta tctacaggga
     3181 ttgtcagctt ctttttaaag atacccgcct tttcgagggt aactgtatct ccgtcataga
     3241 tgaaatacgt tatacgccat cttatgaact gcaacaggaa gaggattggc gcaataacta
     3301 atgccagaac aagtactaca tactttagat ttgaacttac accattctca aaaagctcca
     3361 atataatgaa cactgtgaag ccgatagcac ctttccaggt gttgtctatt acagttacaa
     3421 aactactgcg cctctttatc attttcatcc tcatttctta cttttatatt gacggtgttt
     3481 tttagaaaat ccgctatttt gtatgcgtct tcacgtgcca gaccttccat tttaaagaag
     3541 cccgcagttg tgtacacgac tatggacgcc agattgtggc gtctgagaag aggtccttcg
     3601 tcaattgtca ggtgctggat tcttgaaatg ggtatcatgg atgtttcaat gaatatgatt
     3661 ccttttacta tttctatctt gtcctccgtg aacaaatatt ttctgcgctt gtaacttagt
     3721 tttggtaaac ttgtagctgc ggcaattacc gcagctgcag ttaatacaaa cacagtggtt
     3781 gcgaatattg caaaatcctt gttcccggaa gtgagggcaa atactagtgc tattacatac
     3841 gctataatct gtaatgccaa aagaacatag tatttgaatc tccatacctt catgactttc
     3901 ttgtcaggat gttgcatttt ttgcatactg tctatcatct ccccatatta ttcggaaatc
     3961 cccataagga ttttcgctat atccgaatcc ctgccaagaa caacagtctt gtcatctcca
     4021 gtgagggatg ccttcaatgc ttcaagagaa cgcatgaata tgtagaagtc ctgcttttcc
     4081 tttgtatcaa aggtttctgc aagaagtctc atgtattccg cttcaccttc agctatcaat
     4141 tcttcagctg ttactcttgc ctctgaaatg aggaggctta ctttcttgtc aacttcgttc
     4201 ttcagcatgt ttgcttcaga ctgaccttca gcaagatatc ttgctgcaat ttgatttctg
     4261 tctgatatca ttcttctgta tacagcttcc tcgttggctt gaggcaaatc aaatctttta
     4321 acctttacgt ccattatttc aattccgtaa cctgttgatg tgtttttcac attctggaat
     4381 atttcttcgt tcaaggtatc tctgccggta tcctgctgag ttattattgc attttgttct
     4441 tttcttccta ttacattctt aagtgcgttg tatgtactag catcaagtct tacctgggct
     4501 tctgctattg aacccaatgt tttgtaaaac tggagcgggt ctgatatttt ccaaagtaca
     4561 tagctgtcca cactcattgc ctttgagtcg cttgtcagaa cgtcagaagg tctgagatcg
     4621 taaagctgca gattcttagg aaatttcaga atggtttcaa taaaagggat tctgaagtag
     4681 agtcccgact cgctttttat tgactcaacc tttgaaaagc gaagaatgag ggcgtattcg
     4741 tcctctttta cagtatatag ggaagaggca gccaatatga ttagtatgaa tgcaataacg
     4801 attgccagtg taagtgttac tttctttttc atctcaaata cctccttacg atgtgaattt
     4861 gt
//
LOCUS       DAONVY010000117         4849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12633_length_4849_cov_3.219858, whole genome shotgun sequence.
ACCESSION   DAONVY010000117 DAONVY010000000
VERSION     DAONVY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12633_length_4849_cov_3.219858"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1841)
                     /locus_tag="PLI11_04290"
     CDS             complement(12..1841)
                     /locus_tag="PLI11_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPZ52115.1"
                     /translation="MTKRINFRRTVLVVLTLVLICVSSLAYVPVHSQSNDLYNVIYSG
                     LSSVSGEIDLKPLNLSTSMASDVRDTYFSILYESPELFYVEYQYQIQMVNNTIIKVIP
                     SYKMTGNALANARKEYNQFVDDIVKSVPSGLSDIEKALYVHDYLAANYEYDMDLEIND
                     AYSYFKEGKGVCQSYTLAYMAVLKKLGIEVSYAESDLMNHIWNLIKIDGKWYHVDVTW
                     DDPIMDKPGRVKHDNFLRSDDGIKDTDHYSWNSNYPANSTRYDNYFWGDVDAPFAYVR
                     GSWYYINSSNSTLYKYDFGTNKSTQLFTIQDMWPVVGGGSYWKGCFTGLGEYNGYLFY
                     NTPTKVFAYDTVTGKSMEIATPNTSNGYIYYFTIKNNILTYYQYISPNDDTYVTNGSV
                     NLDTIQFTYTVTYKVDGSVYTTQVYKEGDSIILPAAPEKPGYDFVGWEPNLPGKMPNK
                     DLVVNAVFVKQDCRHESTHVEITEATCTSNGKKETICSICGSILSTEIIPKKDHTYED
                     WIIVKNPTEHEEGLKKKVCSVCKDELTEIIPKLEPVKPTAGEETTRPTDTPVLTESSA
                     TSTPKPTMGAEETHAEETNSPSPTPNVTNTPDTGIEDNEFGAA"
     gene            complement(1838..2341)
                     /locus_tag="PLI11_04295"
     CDS             complement(1838..2341)
                     /locus_tag="PLI11_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPZ52116.1"
                     /translation="MFTYELIETDNKKEFYNRLNKYLDALLGDDRDWLSGLCNASALL
                     YNLMPDINWAGFYLYRDEELVLGPFQGKPACVRIPIGKGVCGSAAASLKVQVVKNTEE
                     FPGHIVCDPASRSEIVLPIVKGDNLIGVLDIDSPVLNRFDMEDAKGLGEFINILFSRI
                     EYNGVLV"
     gene            complement(2527..2862)
                     /locus_tag="PLI11_04300"
     CDS             complement(2527..2862)
                     /locus_tag="PLI11_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52117.1"
                     /translation="MYDSYNSYIVNKNKPYNNQQTLYNNNIDQHQQMVNRMQNEEQLR
                     QFQMQQQLFMDQMQQQEINMQIQNQIELQRQMDEQMQLNMDTHHRMDMQQHMDMQQHV
                     DTHHHMNMM"
     gene            complement(3133..3738)
                     /locus_tag="PLI11_04305"
     CDS             complement(3133..3738)
                     /locus_tag="PLI11_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLAP domain-containing protein"
                     /protein_id="HPZ52118.1"
                     /translation="MKKVFILITVLITVLTVGCARTENDILEYHEQTPSVTDSENKNP
                     GIDESTEDPTKTDGIQETEKPAKTDGVQETDKPQKTEEPANETANPNNVFISNVPNTG
                     DNMVVISPKYVYYDGNAIHMEAFIHNGLSTTVYNLHDVEIKMSNEDGLICDGVFGALE
                     GCVIPSNNYVVWTFYFTEESVLMMNADLSYLITQSFLRYNH"
     gene            complement(3883..4473)
                     /locus_tag="PLI11_04310"
     CDS             complement(3883..4473)
                     /locus_tag="PLI11_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52119.1"
                     /translation="MKKTLLVIIFLAIVFMVGCKDWDDNDTTVGELISETSEPGGKAT
                     NEPVEQTTNNPAEKTNTPGKHPQETPAGDGFNDTNAEGTAVGGNLVITITGYKIDGSF
                     LVVKGIMENKHKTQNIQFSPMDAYVLDGTGKKYPLDLIIHMNDFLDGDIASGERREFE
                     YYFNGYKEADSLTFHISTLYIFDLHYNNERISVKFK"
BASE COUNT         1377 a         1031 c          761 g         1680 t
ORIGIN      
        1 ataactatta actatgctgc accaaactca ttatcctcaa tgcctgtatc aggggtattt
       61 gtcacattcg gtgtgggact tggactattt gtctcttctg catgagtttc ctcagcaccc
      121 attgttggct tgggtgtgct ggtggcactg ctttctgtaa gaactggagt atccgtagga
      181 cgggttgttt cctcccctgc ggtaggttta acaggctcca gttttggtat tatctccgtt
      241 agttcatcct tgcataccga gcataccttc tttttaagac cttcttcatg ctcagtaggg
      301 ttcttaacta taatccagtc ttcatatgtg tggtccttct ttggtataat ctcggtactt
      361 aatatagaac cacatatact gcaaatagtc tccttctttc cattacttgt acatgttgcc
      421 tccgttattt ccacatgggt tgattcatgc ctgcaatcct gctttacaaa cacggcattc
      481 acaacaaggt ccttgtttgg cattttgcca ggcaggttag gttcccatcc aacaaaatca
      541 tatcctggtt tttctggagc tgctggcaaa atgatactgt caccttcctt gtatacttgt
      601 gttgtataca cactgccatc aaccttgtat gtcacagtat atgtaaactg tatggtatca
      661 aggttgacac tgccatttgt gacatatgta tcatcattag gtgatatgta ttggtagtat
      721 gtaagaatgt tgttctttat ggtgaagtaa tagatgtagc cgtttgatgt gtttggcgtg
      781 gctatttcca tgctttttcc tgtaaccgta tcatacgcaa acacctttgt cggagtgttg
      841 tagaacaaat atccattata ctcaccaaga cctgtaaaac aacctttcca ataagagcca
      901 cctcctacaa ccggccacat gtcctgtatc gtgaacagct gagtactttt attggtgcca
      961 aaatcatact tgtacaatgt ggaatttgag ctgtttatgt aataccagct tcctcttacg
     1021 taagcaaaag gagcatccac atctccccag aagtagttgt catacctggt cgaatttgct
     1081 ggataattac tgttccagga gtagtgatcc gtatctttta taccatcatc acttctaagg
     1141 aaattatcat gttttaccct tcctggtttg tccataattg gatcgtccca tgttacatct
     1201 acatgatacc actttccatc aatctttatt aaattccaaa tatggttcat caagtcactt
     1261 tcagcataag acacttcaat gccaagcttt ttcaatactg ccatgtatgc aagcgtgtat
     1321 gactggcata caccttttcc ctccttgaaa tagctgtatg catcatttat ttccaaatcc
     1381 atatcatatt cataattggc agcaagatag tcatgcacat acaaagcctt ttcaatatca
     1441 ctcaatccag aaggcacact ctttacaata tcatcaacga actgattgta ttccttcctt
     1501 gcattagcca aagcatttcc cgtcatcttg tatgaaggaa tcactttgat tattgtgttg
     1561 ttaaccatct gtatttgata ctggtattca acataaaaaa gttcaggact ctcataaaga
     1621 atcgaaaagt atgtatctct aacatcagac gccatcgatg tagacaaatt caaaggcttt
     1681 aaatctattt ctccagacac tgatgaaagc ccggagtata taacgttgta caaatcattg
     1741 ctctggctgt gaactgggac atatgcaagg cttgatacac aaatgagaac cagtgtcaat
     1801 acgaccaaaa ccgtccttct aaagtttatt cgtttagtca taccaacacc ccattatact
     1861 caattcgtga aaacaaaata tttataaact caccaagacc ttttgcatct tccatatcaa
     1921 atctgtttaa cacaggacta tctatgtcca atactcctat caagttatct cccttgacaa
     1981 taggcagaac aatttctgat ctggatgcag gatcacatac aatatgaccc ggaaactctt
     2041 cagtattctt aactacctgc actttcagac ttgcagctgc actgccgcac acccctttac
     2101 ctatcggaat tcttacacat gcaggttttc cctgaaaggg tccaagcacc agttcctcat
     2161 ccctgtataa atagaaacct gcccaattaa tatcgggcat aaggttatac agtaaagcag
     2221 atgcattgca aagcccagac agccagtctc ggtcgtctcc caacaatgca tcaagatatt
     2281 tatttaatct attataaaac tctttcttgt tatctgtctc aatcaactca tatgtaaaca
     2341 taatcaatgc tccaatactg tttatatggt taattataaa gaagtatgga gatatggtca
     2401 aatgaaatag tttactatca agagtctgag tcaccgaact acttccgcca cactatatgg
     2461 tttttttgct acgtttttat tgtcgtatgc tacagctgcg ttctccttaa cgataattcc
     2521 ctattctcac atcatattca tatgatgatg cgtatccaca tgttgctgca tatccatatg
     2581 ttgctgcata tccatacgat gatgcgtatc catattaagt tgcatctgtt catccatctg
     2641 tctctgtaat tcaatctgat tctgaatttg catgtttatt tcctgttgct gcatctgatc
     2701 cataaacaat tgctgctgca tctgaaactg tctaagttgt tcctcgtttt gcattcggtt
     2761 aaccatttgt tgatgctggt ctatgttgtt gttataaagc gtctgctggt tattatatgg
     2821 tttgttttta ttaactatat agctattgta actatcatac atactagaat tattgcaacc
     2881 actacacata caaggaaacc gataacaagt atcatactaa acatattaca actctccttt
     2941 attattttct gtattatatt acatttccgg gacaaaaaat tgcactaatg atattaatat
     3001 aaagtatata ttcattttct ggtgttgcct gttattatga ccgtacggca tattagagac
     3061 attcacagtt ccatttgggt cttaaaagct gtcgcatatt tttctatacg acagcttttt
     3121 gttctaacta ccttaatggt tatatcgcag aaaagattgt gttattagat aagatagatc
     3181 cgcattcatc ataagtactg actcttctgt aaaatagaaa gtccatacaa cgtaattgtt
     3241 tgaaggtata acgcaaccct ctaaagcgcc aaaaacacca tcacatatta gtccatcctc
     3301 attcgacatt tttatttcaa catcgtgcaa attatatact gtcgtactta gtccgttgtg
     3361 aataaacgct tccatatgta tggcattacc atcataatag acatattttg gacttataac
     3421 aaccatatta tcgcctgtat ttgggacgtt gcttatgaag acgttattcg gattagcagt
     3481 ttcgtttgca ggctcttcag tcttttgagg cttatctgtc tcttgtaccc catcagtttt
     3541 tgcaggcttc tctgtctctt gtatcccatc agtttttgtc ggatcttcag ttgattcgtc
     3601 aatcccagga tttttgtttt ccgaatccgt aacactaggt gtctgctcat gatattctag
     3661 aatatcattt tctgtgcgag cacatcctac agttaaaact gttattaata ctgttataag
     3721 aataaaaact tttttcataa gattacctcc cgaagttaat ggtctttgtt gtgtatataa
     3781 taacaaagta gtaagacaaa aaattgcact gtttatatac tattctaagc gtggcatatc
     3841 tgtagcatag aagctaagtc tcataatgta cactagatgt tattacttga acttaacact
     3901 aatcctttca ttgttataat gcaaatcaaa aatatagagt gttgaaatat gaaaagttag
     3961 actatcagct tctttgtaac cattaaaata atactcaaac tcacgtcttt caccggatgc
     4021 aatgtcacca tccaaaaagt cattcatatg tatgattaag tctaatggat actttttccc
     4081 ggtaccatcc aaaacatatg catccatagg gctaaactgt atgttttgag ttttatgttt
     4141 gttctccatt atacctttta caaccaaaaa acttccatct atcttgtatc cggttattgt
     4201 aatcactaga ttaccaccta ctgctgtacc ttcagcattg gtatcgttaa aaccatctcc
     4261 cgccggtgtt tcttgcggat gttttcctgg agtatttgtt ttttccgctg gattgtttgt
     4321 agtctgttca acaggttcat tcgtagcttt tccaccaggt tccgacgttt ctgatattag
     4381 ctcaccaact gtagtatcgt tgtcgtccca atccttgcaa cctaccatga atacgattgc
     4441 tagaaatata attacaagca gtgttttctt catacatagc cctcccccat atattagcgt
     4501 attgtagtac atatctatga tatcaagtat gaaggacaaa aaattgcact attttcaatt
     4561 tactatccgt ttgcatgtag ccttccattt aatatgcgtc aagtccacat ctgtcttttt
     4621 tacatacttg ccttcaaaca ccaatgtata aattactatt tccttagaag caatcttgct
     4681 attcactaca gttgaaaatg ccttcgatgc aataacattc tcgttcttgt caagaattgt
     4741 tattttaata ttttttactc catctatgtc gatatcatag ccattttaaa aaacgcaacc
     4801 atataaagct tttcgtcttt aaaatatata ttcttaggtg aaataatca
//
LOCUS       DAONVY010000118         4840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12662_length_4840_cov_3.688192, whole genome shotgun sequence.
ACCESSION   DAONVY010000118 DAONVY010000000
VERSION     DAONVY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12662_length_4840_cov_3.688192"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLI11_04315"
     CDS             <1..300
                     /locus_tag="PLI11_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52120.1"
                     /translation="GRQLVERHAIKAVELAMEKAEEDKSGNNKIVLSETEFTPADTYT
                     TFNPTPSPTPEETDDGSDGDTEKGGVDPVVIIVVAAVAVIALIVIIIIIRMKKKK"
     gene            477..1571
                     /locus_tag="PLI11_04320"
     CDS             477..1571
                     /locus_tag="PLI11_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52121.1"
                     /translation="MKKTVLLVLAVVLLLSLTAVSQAQESPVITVYKDKTTKIIMAVQ
                     YTEYKDNSGDVQLIIAHDDDRDLYFDGVRAGDSFTYSINVEETGKYELRISFGWLDST
                     GKYTIVVDGEEVGILENTIASTGWRNWTNSDPISIELTKGEHTLTIVNGTPGPNLKYV
                     LLTPEGKEVGDLGTSVHLYDGSASGTPWNLKDNNLSSIAIQFNATSFIKSLKLSAPNW
                     MATPDIDYELEFKLYKWNGSFAKTVSGTSVASRVFEKYPDNTWLYMPVNVEAGEYVLV
                     VTNNGTPNSGFYIKNKAHESQRVYFDGVVKENMAAELIIGYASTPETDYGPLSEDTPE
                     NPETGDSVAVYLLVVVATFGLAFLKKRQDA"
     gene            complement(1607..2527)
                     /gene="prmA"
                     /locus_tag="PLI11_04325"
     CDS             complement(1607..2527)
                     /gene="prmA"
                     /locus_tag="PLI11_04325"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPZ52122.1"
                     /translation="MNWTDIVVEVKTKDKDKAEAIAHMAVPRGLYIEDYSTFEEDLAL
                     TGPIEIVDEELYKKDKESIKIHVYVSPEENPSETLSFLTDRLKSERIGYELNVDNISE
                     EDWANNWKKFFKPTPVGTNLIIVPTWEQENLKTDRVVLLIDPGMAFGSGQHETTKLCL
                     EQIEKYVTKGSKILDVGTGSGILAIAALLLGAGKVTGVDIDPLSIKTAKENAILNKVN
                     ENLELKTGNLATDINETFDIVVANIVADVIIELLPDAYAKLKPEGIFIASGIIELREQ
                     DVLDALSDNGFTPIEAKHDKGWVSITAKRK"
     gene            2614..4590
                     /gene="feoB"
                     /locus_tag="PLI11_04330"
     CDS             2614..4590
                     /gene="feoB"
                     /locus_tag="PLI11_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPZ52123.1"
                     /translation="MKRICAALVGNPNSGKTTLFNNLTGSRHYVGNRPGVTVEKKEGL
                     VKAAGKYGYEVTLVDLPGIYSLSPYSPEEVVTREYILFDKPDVVINIVDATNMERNLY
                     LSMQLIEMEVPMVVALNMMDESRKRGDVIDVSGLSRALNVRFVPISAKTGEGIDDLLK
                     AVFDTVKFHRAVMPEGVYKASVKDLIWKVERLVWKQAIVKNVSSKWASIKLLEGDGLV
                     RETLDLSEETIRRIDRISQVYDMESIIAGCRYGFIEKVVSGAVVRKDNGQATSGKIDR
                     IVANKYLAIPIFLLVMFCVFFLTFGQVGIFMQDCVNVFIKYLGIVTETGLKKLGVQEL
                     IIDLIINGVLAGVGGVLSFLPQIAILFFLISIIEDTGYMARIAFIMDKLLGKIGLSGK
                     AFIPMLMGFGCTVSACLATRTIEDRKGRQMTIMLLPFMSCSAKLPIYGFLAQRFFKES
                     RVWVIFSLYVLGILLMIISGVMFKNTLFKGSDTPFVMELPPYRVPILKNALRHVWERV
                     EHFLKKAATLIFVMSIAVWFLQSFNFKLQYVESSESIISVAGKAISVIFKPLGFGSWQ
                     ASVALLSGLAAKEAVVSTLNMLYGGVHVFKTPLEAYSFLVFTLLYVPCMAAFSTMKKE
                     LGDNKLALLSAMWQTFIAYVVSFLVYSVGKLVLT"
     gene            4645..>4840
                     /locus_tag="PLI11_04335"
     CDS             4645..>4840
                     /locus_tag="PLI11_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52124.1"
                     /translation="MQSKRVVLVALGVFVAVIVLAIVLVVSILSARKSPNLPFNITVT
                     FGMDVYTQKNFCWYTSEEVEI"
BASE COUNT         1350 a          829 c         1124 g         1537 t
ORIGIN      
        1 ggccgccagt tggtcgagcg gcacgcgatc aaggcggtgg aactggcgat ggaaaaggcc
       61 gaggaagaca aatcaggaaa taacaagatt gtcctgtctg aaacggaatt cacacctgcc
      121 gatacatata ccacattcaa tccgactcca tccccaactc ctgaggaaac agacgatggc
      181 tccgatggag atactgaaaa gggtggagtg gatcctgtcg ttatcattgt tgtggctgct
      241 gtggctgtaa ttgctttgat agtgattata attattatta ggatgaagaa gaaaaaatag
      301 gcgaagattg aattatggaa acagccttct tgatatattg ggaggctgtt tttttatgcc
      361 ctttttaata gattaaaata agtcaaatat attgttaatt tatataccaa gtgatgatga
      421 tataatttgt cgagttaaga ggcaaaatta atgagatatc ttggaggtgc attaacatga
      481 agaaaacagt attgcttgtt ttggctgtag tgttgcttct ctcgcttacg gcagtatcgc
      541 aagcacagga atcccctgtt attaccgtat acaaggataa aaccacaaag ataataatgg
      601 ctgtacagta tacggagtac aaggacaata gcggagatgt acagctgata attgcacatg
      661 atgacgatcg ggatctgtac tttgacggtg tacgtgctgg agactctttt acttacagca
      721 tcaatgtcga ggaaactggc aagtatgaac ttcgcatttc ttttggatgg ctcgactcta
      781 caggaaaata caccatagta gttgatggtg aagaggttgg catacttgaa aacaccatcg
      841 ccagtaccgg atggaggaat tggacaaatt ccgatccaat aagcattgag cttacgaaag
      901 gtgagcacac gttgaccatt gtaaatggaa caccaggacc gaatcttaaa tacgttcttc
      961 taacgcctga aggcaaggag gttggtgatt tgggcacgag tgttcatttg tacgatggtt
     1021 ctgcatctgg cacgccttgg aacttaaagg acaacaattt gtcatccata gcaatccagt
     1081 ttaatgctac ttcatttatt aaaagtttga aactttccgc accaaactgg atggctacgc
     1141 ctgatattga ttatgagttg gagttcaaat tgtacaagtg gaatggttct tttgccaaga
     1201 ctgtttcagg tacgtcggtt gcctccagag ttttcgagaa atatcctgat aacacatggc
     1261 tgtatatgcc tgtcaatgta gaagctgggg agtatgttct tgttgttaca aataatggaa
     1321 ctcctaacag tggattctac atcaagaaca aggcgcatga aagtcaaagg gtgtatttcg
     1381 atggagttgt caaggaaaac atggcagcag agttgattat cgggtatgct tccacacctg
     1441 aaacagatta tggacctttg tccgaggata cacctgaaaa tcctgaaaca ggcgattccg
     1501 ttgcagtgta tttgctggtt gttgtggcaa cctttggact tgcttttctg aagaagagac
     1561 aggatgcgta atgtttaagg agccgttttg gctccttgtt atagagttat tttctttttg
     1621 cagtaattga tacccaaccc ttgtcatgtt tggcttcgat tggtgtaaag ccattatctg
     1681 aaagagcatc cagtacatcc tgttctctta attcaattat cccggatgct atgaatatac
     1741 cttccggctt aagttttgca taagcatcgg gtagaagttc aattattaca tctgcgacaa
     1801 tgttggcaac cactatgtca aatgtttcgt taatatctgt agccagattt cctgttttta
     1861 attcaagatt ttcatttact ttgttaagga ttgcgttttc ttttgcggtt ttaatactta
     1921 aaggatctat atcaacaccg gtaaccttgc cagctccgag caatagggct gctattgcaa
     1981 gaatgccgct acctgtacct acgtcaagaa ttttcgagcc tttggttaca tatttttcaa
     2041 tctgctcaag gcacaatttt gttgtttcgt gctgacctga gccaaaagcc ataccgggat
     2101 caataaggag aacaactctg tcggttttca ggttttcctg ctcccatgta ggaacaatta
     2161 tgaggttggt gccaactggt gtgggtttga aaaacttctt ccagttattt gcccagtctt
     2221 cttcacttat gttgtctaca ttcaactcat atccaattct ttcgcttttc aatctatctg
     2281 ttaaaaatga caaggtttcc gatgggtttt cttcaggact tacatataca tggattttta
     2341 ttgattcctt gtcttttttg tacagctctt cgtcaacaat ctcaatgggg cctgtcaagg
     2401 caagatcctc ttcaaaggtg gagtaatctt ctatatacag acctctgggg actgccatat
     2461 gtgcaattgc ttctgccttg tctttgtctt ttgtctttac ttctactact atatccgtcc
     2521 agttcatgat gttttccttt ctgtttttat acaacatata ttttacaatt ctttatccag
     2581 gtatacaagc atatattatt ctgaggtgaa aacatgaaga gaatatgtgc agctcttgtg
     2641 ggcaacccga attcgggaaa gacaacattg ttcaacaatc taacaggcag caggcattat
     2701 gtgggtaaca ggcctggtgt tacagtggaa aagaaggagg gtcttgtcaa ggcagctgga
     2761 aaatatggat atgaagttac tctggttgac ttgcctggaa tatattcatt gtccccatac
     2821 tctccagaag aggttgttac aagagaatac atactgtttg acaaacctga tgtggttata
     2881 aatattgtgg atgctaccaa tatggaaaga aacctgtatt taagcatgca gcttattgaa
     2941 atggaagttc ccatggtggt tgctcttaat atgatggacg agtcaagaaa aagaggagat
     3001 gttattgacg tatcaggatt atctagagcg ttgaatgtga gatttgtacc tatttcggca
     3061 aagacaggtg aaggtatcga tgatttattg aaagcggtgt ttgatactgt gaagttccat
     3121 agggcggtca tgccggaagg cgtgtacaaa gcttcggtta aagatttgat ttggaaggtt
     3181 gagcgtcttg tctggaaaca agctatcgtt aaaaatgttt catcaaaatg ggcatcaata
     3241 aagttgcttg aaggggatgg gttggtaaga gagactcttg acttgtctga ggaaacaata
     3301 aggcggattg acaggatttc tcaagtatac gatatggaat ctattatcgc aggatgtaga
     3361 tacggattta ttgagaaggt tgtaagtggg gcggttgtca ggaaggataa tggtcaagct
     3421 acaagcggga aaatagatag gattgttgca aacaagtatc ttgcaatacc tatttttctg
     3481 cttgttatgt tttgtgtttt ttttcttaca tttggacagg ttggcatatt catgcaggat
     3541 tgtgtaaatg tatttattaa gtatttgggg attgtgacgg agacgggttt gaagaaactc
     3601 ggcgtacagg aactgattat tgatttgatt attaatggag tattggctgg tgttggaggg
     3661 gtattgtctt ttctacccca gatagccata ctgtttttcc ttatatcaat aattgaggat
     3721 acggggtaca tggcaagaat tgcttttatt atggataaac ttcttggaaa gattggttta
     3781 tccggcaaag catttatacc catgcttatg ggatttggtt gtacggtttc tgcgtgtctt
     3841 gcaactagaa caattgaaga tagaaaaggc agacagatga ccataatgct tttacctttt
     3901 atgtcttgtt cggcaaaact gccgatatat gggtttttgg cacagcgttt cttcaaggaa
     3961 agcagggtat gggtgatttt cagcttgtat gtattgggaa tattgctcat gatcatatcc
     4021 ggagttatgt ttaaaaacac attgtttaaa ggtagcgata ctccatttgt gatggagctg
     4081 ccgccttaca gggtaccgat attaaagaat gcattaaggc atgtctggga gagagtcgaa
     4141 cattttttaa agaaggctgc cacattaata tttgtcatga gtattgcggt atggtttctg
     4201 caaagcttca actttaaact gcagtatgtt gaaagcagcg agtcgattat cagcgttgca
     4261 ggaaaagcca tatccgttat tttcaaacct cttggttttg gatcatggca ggcgtccgtt
     4321 gcgctgcttt ccggtctggc ggcaaaagaa gctgtagttt ccactttaaa catgctgtat
     4381 ggtggagtgc atgtattcaa aactcctctt gaagcgtatt cctttctggt cttcacacta
     4441 ttgtatgtgc cttgcatggc tgccttttct acaatgaaaa aagagctagg ggataacaaa
     4501 ttggcgcttt taagtgcaat gtggcagact tttattgctt atgtagtttc tttccttgtg
     4561 tattcagtcg gaaaacttgt tttgacatag caacctcctg gtgataaaat tagtaaaaag
     4621 gcagagaaag cgggggagga taacatgcaa agcaaaaggg ttgtattagt tgcactaggt
     4681 gtatttgttg ccgtaattgt tttggcaatt gttcttgttg tcagcattct ttcagctagg
     4741 aaaagtccca atcttccctt taatataaca gtgacgtttg gtatggatgt ttacacccaa
     4801 aagaactttt gctggtatac ctcagaggaa gttgaaatag
//
LOCUS       DAONVY010000119         4822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12725_length_4822_cov_3.051185, whole genome shotgun sequence.
ACCESSION   DAONVY010000119 DAONVY010000000
VERSION     DAONVY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12725_length_4822_cov_3.051185"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLI11_04340"
     CDS             <1..380
                     /locus_tag="PLI11_04340"
                     /inference="COORDINATES: protein motif:HMM:NF023809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HPZ52125.1"
                     /translation="GMHIYKTHDEQLSSEINQKMQKENRKVPVSVHAGIKQGSPVIIT
                     MSDGKYEVSASGNLVVEKALNRAVNADDVTRQMSKLGGTVFELDYITVDIDENIFVPM
                     SELANIRRELVEKLMEKRTEGAI"
     gene            380..1261
                     /locus_tag="PLI11_04345"
     CDS             380..1261
                     /locus_tag="PLI11_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPZ52126.1"
                     /translation="MKISLYFYTISQYLKCLSVIQEADFVYLPYEYFLSGSTKSKGKT
                     VYVAGPDSTISYNAEDLSREKYFMTFPYILKGKTKTRFINDFGANTLCRHSYLLQNLG
                     DITALQDLCKSHDYNIHADYSFNVQNTKTMDVLYDMGFKSVTLSPELATDKLLNLSTH
                     AESDFLVNEKYTETVCYGRIILMRSEHCYLSDEKGYKCGKCITDDEYSLKDDNGNVYP
                     VLSNPTDCRSILLDRAPYMIKNTDFLEKMRNMRSTILRLNIYDEDASTISALIKYFKY
                     GLSSSETDRIKPYCGRN"
     gene            1271..1720
                     /gene="dut"
                     /locus_tag="PLI11_04350"
     CDS             1271..1720
                     /gene="dut"
                     /locus_tag="PLI11_04350"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPZ52127.1"
                     /translation="MREKIEVYVERCRDDVILPTYANKYDAGMDIRSCVDITIKPNET
                     VIVPTGLKFAIPEGYEIQVRPRSGLSYKTPLRISNAPGTIDAGYRDEVGIIVTNISQT
                     ETIEIKKNDRIAQIVLQRVPEIVFREVDDVSKIGFNRGGGFGSTGKE"
     gene            1732..2208
                     /gene="ispF"
                     /locus_tag="PLI11_04355"
     CDS             1732..2208
                     /gene="ispF"
                     /locus_tag="PLI11_04355"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPZ52128.1"
                     /translation="MEFRCAIGQDSHRFSDKKNKPLVLGGIEFEGKPLLANSDGDVVL
                     HAVTNAISGITGVNILGDIADSMCQQGITDSKEYLKEALKYLDWEIVNLSISIECKIP
                     KISPRIKEMKLSIASLLNISENRIGITATTGEGLTAFGQGLGIQVFCILTVKRSLS"
     gene            complement(2197..3069)
                     /locus_tag="PLI11_04360"
     CDS             complement(2197..3069)
                     /locus_tag="PLI11_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52129.1"
                     /translation="MKTLREAFARVRNRPLILIFPAIATLLLCIIEQFNPFVEKYGSL
                     KTLITLDYMENLAKFAQDVKASAATPGIMVTSIIVFILLISACASIFAVFFSGYAQVL
                     YLSVLGYKPKKGDFKSGINRHFIKMSLLFIFFVLFTIIFIVLMAYTVVPAIMSIKIFF
                     AGDSKIFFQMMLLIILTVMLLYFALVFYVMYWSFSVPGIIGFKRGGVLVALRMVNGYC
                     WYLMPRATLFIFAIGISEVIMLALNYGRGSAGYAIFALFLNWIMKLAIIFPYINFVFS
                     VFIEMKEDMFPSRQ"
     gene            complement(3149..3877)
                     /gene="pyrH"
                     /locus_tag="PLI11_04365"
     CDS             complement(3149..3877)
                     /gene="pyrH"
                     /locus_tag="PLI11_04365"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPZ52130.1"
                     /translation="MQEPIYKRVLIKISGEALAGPDKFGINEDTLNEVCLNIKRIQDM
                     GVDVALVVGGGNFWRGAKSKKMDRTRADHMGMLATVINSLALADNLEKLGVTVRVQTA
                     IEMKQIAEPYIRNRATRHLEKGRVVIFGCGTGNPFFTTDTAAALRAVEIDADVILLAK
                     NVDGVYNCDPNMNDNAVKYEHINYTDVLNKGLGVMDSTATSLCMDNNIVIHVFAIKDP
                     YNMVRAVCGEKIGTIVSWSADSCQ"
     gene            complement(3934..4581)
                     /gene="tsf"
                     /locus_tag="PLI11_04370"
     CDS             complement(3934..4581)
                     /gene="tsf"
                     /locus_tag="PLI11_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPZ52131.1"
                     /translation="MTITAAMVKELRERTQAGMMDCKNALVEANGDMDKAVEILREKG
                     LATAAKKQGRTATEGLVDAYIHGGGRIGVLVEVNCETDFVARNDMFKDFVRDIAMHIA
                     ASNPQYVSVEDVPAEVVEKEKAILKAQAINEGKPEKIVDKIVDGRISKYFEEICLLEQ
                     AFVKDPDKKVKDVVTEMIATIGEKISIRRFARYERGEGLEKKEENFAEEVMKQIQ"
     gene            complement(4600..>4822)
                     /locus_tag="PLI11_04375"
     CDS             complement(4600..>4822)
                     /locus_tag="PLI11_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019115817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPZ52132.1"
                     /translation="GNDDARRAVKLIASTMADAVLEGRQGVSFAKDVEAEEDSFEQAE
                     VEEAADVEETHDEIEAENEAEDDTAEFDE"
BASE COUNT         1476 a         1053 c          885 g         1408 t
ORIGIN      
        1 cgggcatgca tatatacaag acacatgacg agcagttgtc aagtgaaatc aatcagaaaa
       61 tgcagaagga aaacagaaag gtgcctgtta gtgttcatgc tggtataaaa caaggttccc
      121 cggttataat tactatgagt gacggaaaat acgaagtatc agcctctgga aaccttgttg
      181 ttgagaaagc cttgaacagg gctgtcaatg cagacgatgt aacgaggcag atgtcaaaac
      241 ttggcggtac agtgtttgag ctggattata ttactgtcga tattgatgaa aacatatttg
      301 tgcctatgtc ggagctggca aatatcagac gagaacttgt cgaaaaactg atggaaaaga
      361 gaacggaagg tgccatttga tgaaaatctc tttgtacttc tatacaatct cccaatatct
      421 gaaatgcctt tccgttatac aggaagcgga ctttgtatac cttccctatg agtacttttt
      481 gtcaggaagt acaaaatcca agggaaaaac agtctatgtg gctggacccg acagtacaat
      541 tagctacaat gcggaagacc tgtccaggga aaaatacttc atgactttcc catatattct
      601 aaaaggaaaa acgaaaacca gattcataaa cgactttggt gcaaacactc tttgcaggca
      661 cagctattta ttgcagaacc ttggcgacat aaccgcactg caggatttgt gtaaaagtca
      721 tgattacaac atccatgcgg actacagctt caatgtacag aatacaaaaa ccatggatgt
      781 attgtatgac atgggtttca agtcagttac cctatccccc gaacttgcaa ctgataaact
      841 tttgaatctt tcaacacatg cagaatccga ctttctggtt aatgagaaat ataccgaaac
      901 agtgtgctat ggtagaataa tactaatgag aagcgagcac tgctatctat ccgatgagaa
      961 aggctacaaa tgtggaaagt gcataactga cgacgagtac agtctgaagg atgacaatgg
     1021 aaatgtatat ccggttctgt ccaatccaac agattgcaga agcatccttc ttgacagggc
     1081 gccctacatg ataaagaaca ctgattttct tgaaaaaatg agaaacatga ggtccactat
     1141 tctaagactt aacatatatg atgaggatgc cagtacaatc tcagcattaa taaaatattt
     1201 caaatacggt ctgtccagca gtgaaactga cagaataaaa ccttattgcg gaagaaatta
     1261 ggaggtacca atgagagaaa aaattgaagt ttatgtggaa agatgcagag acgacgtcat
     1321 tctgcctaca tatgcaaaca aatatgatgc tggcatggat attcgtagct gtgtagatat
     1381 tactataaag ccaaatgaaa cggtaattgt tcctaccggt ctcaagtttg ccatacctga
     1441 agggtacgaa atacaggtgc gtccccgctc cggtttgtcg tacaaaactc cactcagaat
     1501 ttcaaacgca cccggtacaa ttgatgcagg ctatagggat gaagttggca ttattgtcac
     1561 aaacatatcg caaacagaaa ctattgaaat aaaaaagaac gacaggattg cccaaatcgt
     1621 tcttcaaaga gttcctgaaa ttgttttcag ggaagttgat gatgtttcaa aaatcggctt
     1681 caacaggggc ggaggctttg gctcaacagg taaagaatag gaggaatttt aatggaattc
     1741 agatgtgcca taggacagga ttctcataga ttttctgata aaaaaaacaa gcctcttgtt
     1801 ctgggaggca tagaatttga aggaaaacct cttcttgcca acagtgacgg cgatgtggtc
     1861 ctgcatgcag ttacaaacgc aatctcaggc ataacaggtg ttaatattct gggagatatt
     1921 gctgatagca tgtgccagca aggtataacc gattcaaagg agtatttgaa agaagctttg
     1981 aaatatcttg attgggagat tgtaaacctt tcaatctcca ttgagtgcaa aattcctaaa
     2041 atatcgccaa gaatcaagga aatgaaatta tcaattgcta gtcttttaaa tatatctgaa
     2101 aacagaatag gaataacagc tacaactgga gaaggcctta ccgcattcgg gcaaggcctt
     2161 ggtattcagg ttttctgtat acttaccgta aaacgatcat tgtcttgacg ggaacatatc
     2221 ctctttcatt tctatgaata cgctaaacac aaagtttata tacgggaaga tgatagcgag
     2281 tttcattatc cagttcaaga acagtgcgaa tattgcataa ccggcagaac ctctaccata
     2341 attcaaagcc agcattatca cttcagaaat gcctattgca aatatgaaaa gtgttgctcg
     2401 cggcattaga taccagcagt atccgttaac catccttaag gcaacaagca ctcctcctcg
     2461 cttgaatcca atgattccag gcactgaaaa cgaccagtac attacataga aaacgagggc
     2521 aaaatacaat agcattactg taaggataat caaaagcatc atctggaaaa atatttttga
     2581 atcacccgca aagaatattt ttatggacat gattgcagga accaccgtat atgccattaa
     2641 tacgatgaat ataatcgtaa agagaacaaa aaagataaac agtagactca ttttaataaa
     2701 gtgtctgtta attccgcttt tgaaatctcc ttttttaggc ttatacccaa gtacggataa
     2761 atacagaacc tgtgcatatc cggagaaaaa cactgcaaat atgctggcac atgcacttat
     2821 aagcaatatg aaaacaatta tgctggtaac cattatacct ggggtcgctg cactcgcttt
     2881 tacatcctgt gcaaactttg caaggttctc catgtaatcc aatgttatca aagttttaag
     2941 cgaaccatac ttctctacaa aagggttgaa ctgctcgatt atgcacaaca aaagtgttgc
     3001 aattgctgga aatattaata ttaaaggtct gttccttacc cttgcgaagg cctcccttag
     3061 tgtcttcata aaaacctcca ttcatgccct ggcgggcaca taatattatt acaatgcaga
     3121 ccgtaaaata ttataccact tgcgcaaatt attggcaact gtcagcggac caggaaacta
     3181 tggttcctat tttttctcca cataccgccc ttaccatgtt gtatgggtcc tttatcgcaa
     3241 atacatggat tactatgttg ttatccatgc acagggaagt tgcagtggaa tccattactc
     3301 ccaatccctt gttcaaaacg tccgtgtaat ttatatgctc atacttcaca gcattgtcat
     3361 tcatgttcgg atcgcagttg taaacgccat ccacattctt tgcaaggagt atcacgtcgg
     3421 cgtcaatttc cacagctctc aaagctgcag ctgtatctgt agtaaagaaa ggatttccag
     3481 taccgcatcc gaagataacg actcttccct tctccaaatg tcttgttgct ctgtttctga
     3541 tatatggctc tgcaatctgt ttcatttcga ttgctgtctg tactcttacg gtaacaccca
     3601 atttttctag attgtcagcc agagccagtg aatttattac cgtagcaagc attcccatat
     3661 gatccgcacg ggttctgtcc attttcttgc ttttagcacc tctccagaag tttcctcctc
     3721 ccaccaccag tgcaacatcc acgcccatat cctgtatcct ctttatattc aagcagacct
     3781 catttaaagt atcctcattt atcccaaact tgtctggtcc tgccaatgct tctccactga
     3841 ttttaatcag cactcttttg taaatcggtt cctgcatgaa atattcctcc ttgtttacat
     3901 ataaaaaaag gggaacaaaa gtccccaata tttttattgt atttgcttca ttacttcctc
     3961 agcaaagttc tcttccttct tctcaagccc ttcgcctctt tcatatcttg caaaacgcct
     4021 tattgaaatt ttttctccaa ttgttgcaat catttctgtt acaacgtctt ttaccttttt
     4081 gtcaggatcc tttacgaaag cctgctcaag caagcatatt tcctcaaaat atttggaaat
     4141 tctgccgtca acaatcttgt caacgatttt ttcaggtttg ccttcattga tggcctgtgc
     4201 tttaagaata gctttttcct tttctactac ttcggcaggt acatcctcaa cagatacata
     4261 ttgtggattt gaagcagcta tgtgcatagc gatatctctt acaaaatcct tgaacatgtc
     4321 gttcctggct acaaagtcag tctcgcagtt gacttcaacc agaacaccta ttcttcctcc
     4381 accatgaatg tatgcatcaa caagaccttc tgttgctgtt ctaccttgtt tctttgcagc
     4441 agttgcaaga cccttttctc ttaaaatctc aaccgccttg tccatatctc cgtttgcctc
     4501 aaccaaggca ttcttgcaat ccatcatgcc tgcctgagtg cgctcgcgca actccttaac
     4561 cattgcagca gttattgtca ttcctatacc cccttagtat tattcatcaa actcagctgt
     4621 atcgtcttca gcttcgtttt cagcctctat ttcatcatga gtttcttcaa catctgcagc
     4681 ttcttcaact tcagcctgtt cgaaagaatc ttcttcagct tcaacgtctt tagcaaaaga
     4741 tacgccttgt cttccttcca aaacagcgtc agccatagtt gatgcaatca gttttaccgc
     4801 ccttctagca tcgtcattgc ct
//
LOCUS       DAONVY010000120         4807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12780_length_4807_cov_3.444444, whole genome shotgun sequence.
ACCESSION   DAONVY010000120 DAONVY010000000
VERSION     DAONVY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12780_length_4807_cov_3.444444"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLI11_04380"
     CDS             complement(<1..279)
                     /locus_tag="PLI11_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPZ52133.1"
                     /translation="MDRLRGSEKVAILMIALGQDVSAEIFKHLTEEEIEQITLEITNI
                     RKVDSEVKQQVYSEFYDMYVAQSYISQAGIAYAKEVLEKAIGSQKAYEI"
     gene            complement(284..1858)
                     /gene="fliF"
                     /locus_tag="PLI11_04385"
     CDS             complement(284..1858)
                     /gene="fliF"
                     /locus_tag="PLI11_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPZ52134.1"
                     /translation="MAELIKNIPEKLKEYWSKQSKKKKTMTIVLFAAVILLCAVTIYL
                     INKPEYAVLYSGLSDSEAGEIYRKLTEDDYDVKVGDNGVLYVPKKFENKIRLELAAEG
                     YPKSGLNYDIFSKSSGFGTTQYEKKKYMQFQLQDRLQDTIKSIDLISDAVVTLNIPDN
                     DSVVLKGDKKPTTASVLIKTKKNVKLAREQVDAIVALVSKSVEGLEEENVTVIDQNMT
                     ILNKSNSSDPIFPGTQFELQKQVATDLEEQILNILEPIFGFGKVVTGVNVRLNFDKQT
                     TESIRFEPVIGEDGIIVSLTELIEIRRRLGEDGGDVGQDPNGGAPGYVDPDDENIDYH
                     KISRTVNYEVNQIIELIEKAQGQIEDLTVSVVIDNDDANEEVLTNVRQIVANAVGVDA
                     KYVEVTTMEFAGQKSFEAMFEESRKMQEELFRKQRERTTTIVIVIASAIVLLALIVFI
                     SMAARRASERRRLQQEELMRFYEEQARRQMEEDDNLNFDMIDGQRTDYRDAISKFFRK
                     NPEVFVQLIRNWLNEE"
     gene            complement(1921..2223)
                     /gene="fliE"
                     /locus_tag="PLI11_04390"
     CDS             complement(1921..2223)
                     /gene="fliE"
                     /locus_tag="PLI11_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPZ52135.1"
                     /translation="MNMIGQVAGIQNIGQTNGINTNNSINTNSNFIKIFEDALKMAAE
                     ADQVSKESGTELLDMTVEDLHSVIIDAEKAQLTLGLAVQIRNRVIDAYNEIMRMQI"
     gene            complement(2236..2643)
                     /gene="flgC"
                     /locus_tag="PLI11_04395"
     CDS             complement(2236..2643)
                     /gene="flgC"
                     /locus_tag="PLI11_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPZ52136.1"
                     /translation="MDISASALTAERFRLDVIAENIANMNTTRTAEGGPYRRKTVSFE
                     QIQKPFSEYLDTSFRGNGVRVSAVHEDPSPFKLVYDPNHPDANEEGYVEMPNVDIVTE
                     MIDMISASRAYEANVTALNAYKNIAMRALEIGK"
     gene            complement(2633..3016)
                     /gene="flgB"
                     /locus_tag="PLI11_04400"
     CDS             complement(2633..3016)
                     /gene="flgB"
                     /locus_tag="PLI11_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPZ52137.1"
                     /translation="MANNIFSHIDKFKVGLDASWLRNEILANNIANVDTPNFKRSDVR
                     FSKILEDSISMKTTRDKHINPASSSNYSVEVYEDSNTNVRMDGNNVDIEREMNELAKN
                     AIWYNYLTHMVTKEIKLLDLAINGY"
     gene            complement(3492..4298)
                     /locus_tag="PLI11_04405"
     CDS             complement(3492..4298)
                     /locus_tag="PLI11_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPZ52138.1"
                     /translation="MKISDQDFLKLVQYMLDNYGIDLSKKRTLIESRLSIPVKKMGFD
                     SFSTFVDYAISDNSGKAVNLLTTKLTTNYTFFMREKEHFDFLKESVFPYLESKLEDKD
                     IRIWSAGCSSGEEAYSIAMTIDEYFGAEKFFWDAKVLATDISSTMLSAAIKGVYDESR
                     LEKMPEEWKRKYFKKLDEETYEIKENIKNEVIFRLFNLQDEFPFKRKFHVIFCRNVMI
                     YFNREARISLVSRFYDHLEKGGYLFIGHSESLNGISEDFKYVKPAIYRKI"
     gene            complement(4285..4707)
                     /locus_tag="PLI11_04410"
     CDS             complement(4285..4707)
                     /locus_tag="PLI11_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPZ52139.1"
                     /translation="MNIDIDGYLILVIGSKRALIRLRHVKEIIGMRDFTRVPGLPEYM
                     LGVTNVRGQIVPVCDIARRLSLISNDNRKRSIVIVETSHGDLGLVVDELEGIKRELDL
                     SKVKRVEVRDNDFFKGYYLEGKEELYMLDFMEQANENI"
BASE COUNT         1259 a         1141 c          759 g         1648 t
ORIGIN      
        1 gatttcataa gccttctggc tgcctattgc cttttcaagc acttcctttg catacgcaat
       61 acctgcttgg gatatgtaac tctgggcaac atacatgtcg tagaactcgg agtaaacctg
      121 ctgttttacc tccgaatcca cctttcgtat gttcgtaatc tccagggtta tctgctcgat
      181 ttcctcttcg gtaaggtgct tgaaaatctc tgcagacaca tcctgtccca atgctatcat
      241 cagtattgca accttttcac tgcctcttaa tctatccatt aactcactcc tcatttagcc
      301 agtttcttat gagctggacg aatacttctg gattctttct gaaaaatttc gagattgcat
      361 ccctgtaatc agttctttgc ccatcaatca tgtcaaaatt caggttgtcg tcctcttcca
      421 tttgcctgcg tgcctgctct tcatagaacc tcataagttc ctcttgctgc agccttcttc
      481 tttcacttgc cctgcgcgca gccatggata tgaaaactat cagtgcaagt agtacaattg
      541 cacttgctat aacaattaca atagttgttg ttctttctct ttgttttcta aacagttcct
      601 cctgcatttt tcttgattct tcaaacatgg cctcaaagga tttttgtcct gcaaactcca
      661 tggtggtcac ttcaacatac tttgcgtcca caccaacagc gtttgcaaca atctgcctta
      721 cattagtcaa aacctcctca tttgcatcat cattatcaat aactacggag actgtaaggt
      781 cttcaatctg cccctgcgcc ttttctataa gttcaataat ctggttaact tcataattga
      841 cggttctact aatcttgtga taatcaatgt tttcatcatc cgggtccaca tagccgggag
      901 ctcctccgtt aggatcctgt cccacatcgc cgccatcttc acctagtctt ctccttattt
      961 caataagttc tgtcaagctt acaattatac cgtcctcgcc aatgacaggc tcgaaacgaa
     1021 tactttctgt ggtctgcttg tcaaagttga gccttacatt cacacctgtt acaaccttgc
     1081 caaaaccgaa gataggttcg agaatattca aaatctgctc ttccaaatcg gtggctacct
     1141 gtttctgaag ctcgaactgg gtgccgggga aaatggggtc gcttgaattg ctcttgttga
     1201 gtattgtcat gttctggtca ataactgtga cattttcctc ttcaagacct tcaacactct
     1261 ttgaaacaag cgctactatt gcatctacct gttctcttgc tagctttaca ttcttctttg
     1321 tttttatcaa aaccgatgcg gtggtgggtt tcttgtcacc cttgagaaca acggagtcat
     1381 tgtcaggtat gttcaaagtg accacagcat cacttatcaa atcaattgat ttgatggtgt
     1441 cctgcagcct gtcctgaagt tgaaactgca tgtatttttt cttttcatac tgggtcgtgc
     1501 caaaacctga actttttgag aatatatcgt agttcaagcc gctctttgga tagccttccg
     1561 cagccaattc cagtcttatt ttgttctcaa actttttggg cacataaaga acgccgttgt
     1621 ctcctacttt tacatcatag tcgtcttcag tcagctttct gtatatttca ccagcctcgc
     1681 tgtctgaaag acctgaatac aaaacggcgt attccggttt atttattaag tatatggtaa
     1741 ctgcgcaaag taatataacc gcagcaaaca atactattgt catcgttttt ttcttctttg
     1801 attgctttga ccagtactcc ttaagtttct caggaatatt ctttatcaac tcagccattt
     1861 cctactctgt ttcctatccg ttatttttga taattaatat aactaactat ttggggaaaa
     1921 ttatatttgc attctcataa tttcgttata cgcatcaatt accctgtttc taatctgaac
     1981 tgccagaccc agggtcagct gcgccttctc cgcatcaata atgacggagt gcaaatcctc
     2041 cacggtcata tcaagaagtt ccgtaccaga ctctttgctg acctgatctg cttcagccgc
     2101 cattttaaga gcgtcttcaa agattttaat aaaattcgaa tttgtgttga tggaattgtt
     2161 tgtgttgata ccatttgttt gtcctatgtt ctgaataccc gcaacctgcc caatcatgtt
     2221 catttaatta acctcctact tgccgatttc cagtgctctc atcgctatat tcttgtatgc
     2281 gttcaatgcg gtcacattcg cctcatatgc cctgctggca gatatcatat caatcatttc
     2341 agtgacaata tccacattgg gcatttcaac atagccttcc tcatttgcat ccggatgatt
     2401 cggatcatat accagcttga agggacttgg atcctcatgc acagcactta ctcttacgcc
     2461 gtttcctcta aaagaagtgt ccagatattc ggaaaaaggc ttttgaatct gctcaaaaga
     2521 tactgtttta cgtctatacg gaccgccctc ggccgtcctg gtagtattca tgttggctat
     2581 gttttcagca attacatcca atctgaaacg ttccgctgta agtgctgagg cactaatatc
     2641 cattaatcgc caagtctaac agttttattt ccttggtcac catatgggtt aaatagttgt
     2701 accaaatggc gttttttgca agctcattca tctcccgttc aatgtccaca ttatttccat
     2761 ccatgcgcac attggtgttg ctgtcctcgt aaacttcaac tgaataattt gacgaggaag
     2821 cgggatttat atgcttgtct cttgtagttt tcatgcttat tgaatcttca agaattttag
     2881 aaaacctgac gtctgatctc ttgaaattgg gcgtatcaac attggctatg ttgttagcca
     2941 atatttcgtt tctcagccat gaggcatcga gcccaacctt aaatttgtcg atgtgactga
     3001 atatattgtt tgccatttcc cctacccctt acattactac atatttttca catattgtta
     3061 accaatttaa gctatattat agtaacacta ttacagatat tactatacag gcatatacca
     3121 tgtcaatcaa tttatcctgg tttgttccaa actgcaatat gctgacagtc agctggtaat
     3181 ccttatatta ttttcagtct tttccatttt ctagaacccg tttttaatag ttttcacctt
     3241 tcaaataaaa atacaattaa taatttattc acatcatgat taagagaatg tattaccgca
     3301 agtgtgtgta taaaaatatt tcacatattt tggccacgac taaagatgca gcctgtttta
     3361 atttctaaag aatatatcac ataaatctac caatgtcaaa atgtttttat ttgttcacaa
     3421 ttaaaaaaag cacagagcca ttattaataa ttggctctgt tcctttttta tgaaaatata
     3481 ttatttttat cctaaatctt ccgatatatt gcaggtttta catatttaaa atcctccgat
     3541 atcccgttta gcgattccga gtgccctata aacaaatatc cgcccttttc aagatgatca
     3601 taaaatctgc ttacaaggct tattctagct tccctgttga aataaatcat gacattcctg
     3661 cagaagatta cgtgaaactt tcttttaaaa gggaactcat cctgaagatt gaataatctg
     3721 aaaatgactt cgttttttat attctccttt atctcataag tttcttcatc caactttttg
     3781 aaatactttc tcttccactc ctcaggcatc ttctcaagcc tgctttcatc atacactccc
     3841 tttattgctg cactcaacat ggtgctggat atgtcggtgg caaggacttt ggcatcccag
     3901 aagaactttt cggccccgaa atattcgtca atcgtcattg caatcgaata cgcttcttct
     3961 cccgatgaac agcccgcact ccaaatccta atgtccttgt cctcaagttt tgactcaaga
     4021 tacggaaaca cgctttcctt caagaaatca aaatgctcct tttctctcat aaaaaaggtg
     4081 tagtttgtgg taagctttgt tgtaagcaaa ttcacagcct tgcccgaatt gtcgcttatg
     4141 gcatagtcaa caaaagtgct gaaggaatca aaacccatct ttttgacggg tattgacagt
     4201 ctactttcta taagggtacg tttcttgctc aagtcaatgc cgtagttgtc cagcatgtac
     4261 tgtacaagtt tcaaaaaatc ctgatcagat attttcattt gcctgctcca tgaaatccag
     4321 catatacaat tcctcttttc cttcaaggta gtatcccttg aagaaatcat tatccctgac
     4381 ctccaccctc tttaccttgg aaaggtccag ctccctttta atgccttcca gttcatccac
     4441 cacaaggccc agatcgccgt gactggtttc aacgataact atgcttcttt tccgattatc
     4501 attggagata agggacaacc tcctggcaat atcacagact ggaacaatct gtccccttac
     4561 attagtaact cccaacatgt actctggaag acctggaacg cgtgtaaaat cacgcattcc
     4621 aataatctcc tttacatgcc ttaaccttat taatgctctt ttgcttccta taaccaatat
     4681 cagatatcca tcaatatcga tattcataat tctctccatc ttaatatttt ccaaagtcat
     4741 ctgcattctc tataagatcg cagcggtcag ccttgtatac ttccttcaat ttaaactggt
     4801 ttttaac
//
LOCUS       DAONVY010000121         4778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_12867_length_4778_cov_3.459030, whole genome shotgun sequence.
ACCESSION   DAONVY010000121 DAONVY010000000
VERSION     DAONVY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12867_length_4778_cov_3.459030"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLI11_04415"
     CDS             <1..334
                     /locus_tag="PLI11_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52140.1"
                     /translation="ILANYAFDADLIALLHDNPEEGSKEIMDLIEKTYPEGETPRPDV
                     TPTPESTPEPTTTAEETEVPKATSTAKTQSPKKDKDNTYLVPVIIGGTVVIAGVVLFL
                     TLGSRKKK"
     gene            368..4504
                     /locus_tag="PLI11_04420"
     CDS             368..4504
                     /locus_tag="PLI11_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52141.1"
                     /translation="MVRLVVLVLCFALLTASMYGQVSARSLADREKDLYLKPGPSPMD
                     KKPDIVTLKEGDLVSRVQAKSILLDGEWLLTDKGMDKWDNAYKVNVPGSILHGLHQAG
                     VIKDPYMGKNDFAAKAQTEKTWYLKKTFSYSGSKKNVELYFHGVADRCDVYLNGSRIA
                     SHQGMFGGPRVDVSDSLKIGQNELVVVLKPVLPWQQTVVFNCSYGWHYSIIPPMGIWN
                     SVEISDLPDVEFDSPFIATYSYKKGTMDLSIDLRAVDKDASMKGVLTGTIKPKNFQGK
                     TFSFTYNINGEKGKDANVRLRFDMPEFKLWWPNGYGEQNLYTLELSFKSDSGEVTYSK
                     TSFGIRELVLDPFPTGETESMYNRTVVINGRKLFMKGAGWCTIDALMRFTREDYDRIL
                     RRAKEQGLNFLRAWGGGMPETDDFYDLCDEYGITVYQEWPSCWDSHDSQPANVFYETV
                     ILNTKRIRNRPSLIIYGGGNEGAGAFESVTLNNIGKLTYEYDGTRTFYRQDGGPGGAG
                     MGHDHIHWGGQSPEHYITAYANRLYLNLHEYGLDSMMNLESIARYATKEEMDKWPIDP
                     EGTIAHHTATFNGNLGWNESPHGHDVDTLMHYASYFLNVKSLKDLVIGTQLAQTMATY
                     LPIQNARINFPNVTKIAYYKLNDVFPGASWSTVDWYGVPKIAHYFVQDANRTLMATGH
                     FDRYNTYDKQQKDFNLPIYILDDNGDLKNSSWKVIVKAYDKFLNVVKSEEFSGSGGVG
                     TNKHIGDFYLTEKEADSSPLFIVIDLVKDGDEPIRSYTFMNFDRIKGSLFTLPRTTLE
                     YSVSGNRFTVKNTGNVPAVSVNFKNSKVSDVFWCEDNYFWLEPGESKTIEVNMTDGVD
                     GFDAFNLSDADDKKAPGQVPKLEAKSTSSSVTLSWRSAKDDKKVMGYYIYMNDVLVDF
                     VGSDYLTYTVTGLDENTEYTFYVVAVDYNMNESKASKKITVTTKPDTERPYAVSVKVV
                     DRNTVVVRYNKDMNKESAEDKINYAFNFDVQINSISLAEDLRTVTIKTSDMDFESNKR
                     YSLLVLGARDSTYTGNQAARRKFYLHESLAGYWSFDEGKGDCVHDLSGKYEEPGVIAK
                     AEWTEGVSGSALDFDKLGNVYVGMSDFYVSDGATISMWLKPSDLSGFGVIIAKGPKEA
                     GHFEIYSKDGNLLVFSPDVGDLALKVNMSSMVDTWNHFAFIINQSRIMTYVNGELAST
                     TAVGRFKDVDGDLAIGALADASLPFGGAIDEVYLFERELDVDEISYLAMKSGDIRDMV
                     LNENFRKMNIGEVFKLNAVFHPEGVEGRNIVWSVDNAEVASIDNGVVTALDYGFAVVT
                     VMTEDGEFIDRCFVEVGDFKKKVDPVLITAICCGVFLILIILFSISYKFYMKRKKNMV
                     "
     gene            4537..>4778
                     /locus_tag="PLI11_04425"
     CDS             4537..>4778
                     /locus_tag="PLI11_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPZ52142.1"
                     /translation="MNEEVKRLLNEQINKELYSSYLYLSMADYYDYNNLDGFANWFYI
                     QAQEEVDHAMLLRRYLLNNGEAIELEAIAKPDLVFNS"
BASE COUNT         1440 a          803 c         1212 g         1323 t
ORIGIN      
        1 catactggca aattacgctt ttgatgctga cttaatagct cttttgcatg acaatcctga
       61 agaaggctcg aaggagataa tggacttgat tgagaagacg tatcccgaag gtgaaacacc
      121 aagacctgat gttacaccaa ctcccgaatc aactcctgag cctacaacta ctgctgagga
      181 gacagaggtt cccaaagcca catccacagc aaaaacccaa tctcccaaaa aggacaagga
      241 caatacatat ttggtaccag taattattgg aggaacggtt gttattgcgg gtgttgttct
      301 gtttctgacc cttggaagca ggaagaagaa atgaaaacct ttcacagata ttaaggagga
      361 taaagatatg gtaaggcttg tggtgttggt gctttgtttt gcattgttga cggcatctat
      421 gtacggacag gtgtctgcaa gaagtcttgc agacagggaa aaggatttgt atttgaaacc
      481 tgggccatct cctatggaca agaagcctga tatcgttaca ttgaaggaag gggacttggt
      541 ttcaagagtt caggcaaaga gcatcctttt ggatggagag tggcttctta ctgacaaggg
      601 tatggataaa tgggacaatg cgtacaaggt aaatgttcca ggcagcattt tgcatggtct
      661 gcatcaggcg ggagttataa aggatccgta tatgggaaag aatgactttg ctgccaaagc
      721 tcagacggaa aagacctggt atttaaaaaa gacgtttagt tatagcggat caaaaaagaa
      781 tgtggaactg tattttcatg gggttgcaga cagatgtgac gtatatctga atggcagcag
      841 aattgcaagc catcagggaa tgtttggagg tccccgggtt gatgtttcag acagcttaaa
      901 aattggacaa aatgaacttg tagttgtatt gaagccggtc ctgccatggc aacagacagt
      961 tgtgttcaac tgttcttacg gatggcatta ttcgataata cctccgatgg gtatttggaa
     1021 ctctgttgaa atttcggatt tgcccgatgt tgaattcgat tctccattca tagccactta
     1081 ttcgtacaaa aaaggaacaa tggatcttag catcgatcta agagcggtgg ataaggatgc
     1141 ttcaatgaaa ggtgtgttga caggcaccat aaaaccaaag aatttccaag ggaaaacctt
     1201 ctcttttaca tacaatataa atggtgaaaa gggcaaggat gccaatgtta gattgagatt
     1261 tgacatgcca gaattcaaac tctggtggcc taatggttat ggggagcaga acttatatac
     1321 actggagttg tctttcaaat cagattcggg agaggttaca tattccaaaa cttcttttgg
     1381 cattagagag cttgtacttg atcctttccc gacgggtgaa acggaaagca tgtacaacag
     1441 gaccgtggtc ataaacggaa ggaagctgtt tatgaaaggt gctggctggt gtacaatcga
     1501 tgctcttatg cggtttacaa gggaggatta tgacaggata ttaagaaggg caaaggagca
     1561 gggtcttaac ttcttgagag cctggggtgg aggaatgcct gagacggatg atttttatga
     1621 tttgtgtgat gaatatggaa ttaccgttta tcaggaatgg cccagctgct gggacagtca
     1681 tgattcacag cctgccaatg tgttttatga gaccgttata ctaaatacaa agaggattag
     1741 gaaccgtccg tctttgatta tttatggcgg tggaaatgaa ggtgctggtg cttttgaaag
     1801 cgtgacgctc aacaatattg gaaaacttac atatgaatat gacggaaccc gtaccttcta
     1861 ccgtcaggat ggaggtcctg gaggagcggg catgggtcat gaccatatcc actggggcgg
     1921 acagtctcct gaacactaca ttacagcgta tgccaacagg ctttatctta atctgcatga
     1981 gtacggactt gattcgatga tgaatctaga gtccattgcc aggtatgcca ccaaggagga
     2041 aatggataaa tggccaatag atcctgaagg aacaatagct catcataccg ccactttcaa
     2101 tggtaatttg ggatggaacg agtcgcccca cggtcatgat gtggataccc tcatgcatta
     2161 tgcaagttat tttcttaatg ttaagtcttt aaaggacctt gttatcggca cgcagctagc
     2221 ccagaccatg gcaacatatc tgccgataca gaatgcaaga atcaacttcc caaacgttac
     2281 gaaaattgca tattacaagc ttaatgacgt attccctggc gcaagctgga gtacagttga
     2341 ctggtatgga gttcccaaaa tcgcccacta ttttgtccag gatgccaata gaactttaat
     2401 ggcaacgggg cattttgaca gatacaatac atatgacaag cagcaaaaag attttaatct
     2461 tccaatatat attttggatg acaatgggga tttaaagaat tcatcgtgga aggttattgt
     2521 aaaagcatat gataaattcc tgaatgtcgt taagagcgag gagtttagtg gcagtggagg
     2581 agtaggaacc aacaagcata ttggggactt ctatttaact gaaaaggaag cggattcatc
     2641 tccgttgttt atagtgattg accttgtcaa agatggtgat gagcccataa gaagctacac
     2701 attcatgaat tttgacagga ttaaggggag tcttttcaca ctgccaagaa caactcttga
     2761 gtatagcgtg tcaggaaacc gttttactgt aaagaatact ggaaacgtgc ctgcagtgtc
     2821 tgtcaatttt aaaaactcaa aggtgtcgga tgtattctgg tgtgaagaca actatttctg
     2881 gcttgaaccg ggtgaatcca aaaccattga agtcaatatg actgacgggg ttgatggatt
     2941 tgatgccttc aatctatcag atgcggatga caagaaagct ccgggacagg ttcccaagct
     3001 ggaagcaaaa tcaacatcca gttccgtaac ccttagttgg aggagtgcaa aggatgacaa
     3061 gaaagtcatg ggctattata tttatatgaa tgatgtgctt gtggattttg ttggttccga
     3121 ttacctaacg tatacagtaa caggacttga cgaaaacacc gagtatacat tttatgtggt
     3181 ggcggtagat tacaacatga atgagtccaa ggcttcaaaa aagattactg taactacaaa
     3241 gcctgatacg gaaagaccgt atgctgtttc tgtaaaggtt gtggacagaa atacagtggt
     3301 tgttagatac aacaaggata tgaacaagga aagtgctgaa gataagataa actacgcatt
     3361 taattttgat gttcagatta attccataag tcttgcggag gatttgcgga ctgttacaat
     3421 taaaacctcg gatatggatt ttgaaagtaa taagagatac agtctgctgg ttttgggtgc
     3481 aagagacagc acatatactg ggaatcaggc agcaagaagg aaattctatt tgcatgaaag
     3541 ccttgctgga tattggagct ttgatgaagg gaaaggagat tgcgttcatg acttgagtgg
     3601 aaaatatgaa gagccgggtg ttattgcaaa agccgaatgg acagaaggcg tatcaggcag
     3661 tgctcttgat tttgacaagc ttggtaatgt ttacgtgggg atgtccgact tttatgtgtc
     3721 ggatggcgct accatatcca tgtggctcaa gccttcggat ttgagcggat ttggcgtaat
     3781 tattgccaaa ggtccaaagg aagcgggaca ttttgagatt tactcaaaag acggaaatct
     3841 gcttgtattc tcaccggatg tgggagatct ggcgttgaag gtaaacatgt catccatggt
     3901 ggatacctgg aaccactttg cctttataat caatcagagc aggataatga catatgtaaa
     3961 tggtgagctg gcatccacca ccgctgttgg aaggttcaag gatgtggatg gagatttggc
     4021 tattggagcg ttggcggatg cttcattacc gtttggtggt gcaattgatg aggtttactt
     4081 gtttgaacgg gagcttgatg tggatgagat aagctatctt gccatgaaat ccggagatat
     4141 tagagacatg gtgctgaatg agaacttcag gaagatgaat attggtgagg tattcaagct
     4201 gaatgcagta ttccatccag agggcgttga aggcaggaat attgtttggt ctgttgacaa
     4261 tgccgaggtg gcaagcattg ataatggggt tgttacggcg cttgattatg gttttgctgt
     4321 tgttacagtc atgactgagg atggagagtt tattgaccgg tgtttcgtag aagttggaga
     4381 tttcaaaaag aaggtggatc cggttctgat tactgcaata tgctgtgggg ttttcttaat
     4441 acttatcata ttgttttcga taagttataa attctacatg aaaaggaaga aaaatatggt
     4501 ataatatgtg catatacaaa aaggaggaga gttcttatga atgaagaagt taaaagacta
     4561 cttaatgagc aaataaacaa ggagttgtat tcgtcatatc tttatctcag tatggctgat
     4621 tactacgact acaacaatct cgacggtttt gccaactggt tctatatcca ggcacaggaa
     4681 gaggttgacc atgccatgtt gcttagaagg tacttgttga acaatggaga agccattgag
     4741 cttgaggcta tagcaaagcc tgatcttgtg ttcaattc
//
LOCUS       DAONVY010000122         4736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13008_length_4736_cov_3.966460, whole genome shotgun sequence.
ACCESSION   DAONVY010000122 DAONVY010000000
VERSION     DAONVY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13008_length_4736_cov_3.966460"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLI11_04430"
     CDS             complement(<1..454)
                     /locus_tag="PLI11_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase, N-terminal
                     domain-containing protein"
                     /protein_id="HPZ52143.1"
                     /translation="MLDTVERVKGMYKVEETKKGIKVFGVKDFNPKHLFECGQCFRWN
                     LNSRGHYIGVAGGRYAEIVYSDGVLEIIGTTKEDFYTFWFDYFDLGRDYSRIKEELSE
                     KDEYMRQAVQFGSGIRILRQEPFETLISFIISANNNIPRIKKCIENLCR"
     gene            548..1294
                     /locus_tag="PLI11_04435"
     CDS             548..1294
                     /locus_tag="PLI11_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HPZ52144.1"
                     /translation="MKETIYTIPVNEVFAEKCECPLCELESRFEKETVDYYLGPSLME
                     PDNRIETNETGFCARHYGMMYNTQANRLGLGLILDTYLLEQNKKLSKLMRVESKSQGE
                     KKGLFGRGKKTSSNVDEVYKYISEHLEECCICSKLSYTMSRYIEVISHLYVREKEFRE
                     KFNDGLGFCMPHLKLLIEGAKKHLSSSQQDEFFKNLFSMQINNLERLEKEVSWFTQKF
                     DYRNNDKPWGTSKDALIRGIKKVSGLKELD"
     gene            1308..2141
                     /locus_tag="PLI11_04440"
     CDS             1308..2141
                     /locus_tag="PLI11_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52145.1"
                     /translation="MEVYALVGASGTGKSHRALWVAKERNIEYIIDDGLLIKGNEIIA
                     GKSAKREKTRIGSIKTAVFTSDDHAFEVALQLKLRNAKSVLILGTSDNMVKRIAERLG
                     LNKIDKTIYIEEISSKFEIQQALNTRKTEGKHVIPVPTLELKKDFSGFLLDPLNILRR
                     KGIGDYESLGEKSVVRPTFSYLGKFTISDYTMYQLVEHIALKEEGVAKLGRFRAETSE
                     SGISLNIEFVLYYGYNIPELFKKLKARIIEEIERITSLNIDAIVLIAKSLVVKPKSKA
                     N"
     gene            complement(2138..2887)
                     /locus_tag="PLI11_04445"
     CDS             complement(2138..2887)
                     /locus_tag="PLI11_04445"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HPZ52146.1"
                     /translation="MYPFRITSVSNREELVRFLMKKLPGARYDEPFVKCRIGINQISK
                     VLAEYIWDYVLIRKIKYYLIKSKIFEKEDRYVVFERIIDVASCIRVQPDLVEHSLSEY
                     LRMHRTIAVDGFANFRLRELINRVKALSDICISEYIAQKEYEEYIELLKNFVKQQKSE
                     YKEVHIVTGEDGQHRIYNDEGMDITFKCLEGFVDSKMAINASLDDLMVTTLIAVAPNR
                     IIIHNENKSRNPELVETIKGVFAGRIIISNN"
     gene            complement(2949..3656)
                     /locus_tag="PLI11_04450"
     CDS             complement(2949..3656)
                     /locus_tag="PLI11_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52147.1"
                     /translation="MNLYFVLSCQADEKIIEELKKYGNVILVYPHPNLHEAVSCHADM
                     QLAKIDNKVLVYAKGINQRTLDALYDTGIELIEGGTQLLPKYPGNIAYNVLKVGRNIY
                     HNTKYTDCIVRKVFTDKGYDFVNVRQGYAGCSSIYVKNFILTTDKGIEKKAKEMGDRC
                     FWFNGDDIILKGYDHGFIGGCSGFIEDLGLLLTGSLEYHKEGRLVKEFLLEQGVNILE
                     LKNDLLEDIGGIQIYKL"
     gene            3806..4024
                     /locus_tag="PLI11_04455"
     CDS             3806..4024
                     /locus_tag="PLI11_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HPZ52148.1"
                     /translation="MARTGFDRFALILVIIGAINWLSVGLFRFDIVAALFGGATNLVS
                     RIIYSVVGIAGIYSISLLFRHSPVNVRE"
     gene            4105..4299
                     /locus_tag="PLI11_04460"
     CDS             4105..4299
                     /locus_tag="PLI11_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPZ52149.1"
                     /translation="MARSRNKTTFDKLKYEIANETGINLKEGYNGDLTSREAGTIGGN
                     IVRKVFESYTGNNYYTGKPQ"
     gene            4412..4678
                     /locus_tag="PLI11_04465"
     CDS             4412..4678
                     /locus_tag="PLI11_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52150.1"
                     /translation="MIRIIVVFAVTTLFVFFPEIFPRCEYCRKIKLRKCFQFHKSVSL
                     KLTYKGNLSLCKKCCKKYNFTSLDKFRKHMRVEKRIEYTVRYNL"
BASE COUNT         1527 a          929 c          845 g         1435 t
ORIGIN      
        1 ttctgcaaag gttttcaata cacttcttta ttctcggtat gttgttgttt gcagagatta
       61 taaaagatat gagggtttca aaaggttcct gacgcagtat cctgattcca ctgccaaatt
      121 gtactgcctg cctcatatac tcgtcttttt cagatagttc ctcttttatt ctcgaataat
      181 ctctgccaag atcgaaataa tcaaaccaaa aagtataaaa atcttctttt gtggtaccaa
      241 taatctcaag aaccccatca gaataaacaa tctctgcgta tctgccacct gccacaccaa
      301 tatagtgtcc tctggagttc aaattccacc tgaaacattg tccgcattcg aataagtgtt
      361 tggggttaaa gtcttttaca ccaaatactt ttattccctt tttcgtttcc tcaactttgt
      421 acatgccctt cactctttca actgtatcaa gcatcaattg tacgccaaca taactgcatt
      481 gtcaatcact cgattctgtg ctagaatagt tgttgaccac atttatattg gaggtaccaa
      541 aagactcatg aaagaaacta tttatacaat ccctgtaaac gaagtttttg ctgaaaaatg
      601 cgaatgtccc ttgtgtgaat tggaatcccg atttgaaaaa gaaaccgttg actactacct
      661 gggaccttct ttaatggagc cggacaaccg aattgaaaca aacgagactg gtttctgtgc
      721 aaggcattat ggtatgatgt acaacacaca agccaaccgc ttggggcttg gtctcattct
      781 tgacacttat cttcttgagc aaaacaaaaa attgtccaaa ctaatgaggg tcgaaagcaa
      841 atcccagggt gaaaaaaagg gtcttttcgg caggggcaaa aaaacctcca gcaatgttga
      901 tgaagtatac aaatacattt cagagcatct tgaagaatgc tgcatatgca gcaagttgtc
      961 atatacaatg agcagatata ttgaggtaat atcccatctc tatgtcagag agaaagagtt
     1021 cagagaaaaa ttcaatgatg gacttggctt ttgcatgccc catctgaagc tgctgattga
     1081 aggcgcaaaa aaacacttga gttcatccca acaggacgaa tttttcaaga atttgttctc
     1141 gatgcagatt aacaatcttg aaagacttga aaaggaagtt tcctggttta ctcaaaagtt
     1201 cgactaccga aacaacgaca agccttgggg aacttccaaa gatgcattga taagaggtat
     1261 taagaaagta tcgggattga aagaattgga ttaagaggtg tagtgatttg gaggtatatg
     1321 cgcttgtagg ggcaagtggt accggaaaaa gccacagagc attgtgggta gcaaaagaaa
     1381 gaaatattga atatatcatt gatgatggcc ttttaataaa aggcaatgag attattgccg
     1441 gcaaatccgc caagagggaa aagacccgca ttggctccat caagactgcc gtttttacaa
     1501 gcgatgatca cgcttttgaa gttgccctgc agttaaagct gcgcaatgca aaaagtgttc
     1561 tgattcttgg tacttccgac aacatggtaa aaagaattgc tgaaaggctt ggtctaaaca
     1621 agattgataa aacaatatac attgaagaaa tatccagcaa gtttgaaata cagcaggcgt
     1681 taaatacaag aaaaactgaa ggaaaacatg taatacccgt acccaccctt gaattgaaaa
     1741 aagacttttc gggtttcttg ctggatcctt tgaacatatt aagaagaaaa ggtataggcg
     1801 attatgaatc cttgggcgaa aagtccgtgg ttagacctac attcagctac ctaggcaaat
     1861 tcaccatatc cgactacact atgtatcaac tggttgaaca tattgcgctg aaagaagaag
     1921 gcgttgcaaa actcggaaga ttcagggctg aaacaagcga atcaggaatc agcctgaata
     1981 ttgagtttgt attgtactac ggatacaata taccagagtt gtttaaaaaa ctgaaagcaa
     2041 gaattattga ggaaattgaa agaataacgt ccttgaatat agatgccata gtccttattg
     2101 caaaaagtct tgtcgtcaaa ccaaaaagca aggccaatta gttgtttgat attattatcc
     2161 ttcctgcaaa aacacccttg atggtttcca caagttccgg atttctgctc ttgttttcat
     2221 tgtgaataat aattctattg ggtgcaacag caattaatgt tgtaaccatc agatcatcaa
     2281 ggcttgcatt tatggccatt ttgctgtcca caaaaccctc aaggcatttg aatgtaatat
     2341 ccataccttc atcattgtat attctatgct gaccatcttc cccagtaaca atatgcactt
     2401 ccttgtattc ggatttttgt tgtttgacaa aattcttcag aagttcaata tattcctcat
     2461 attccttttg agcaatatat tcgcttatgc aaatatctga caaagccttt actctgttta
     2521 taagttctct caatctgaaa tttgcaaatc cgtcaactgc tatggttctg tgcattctca
     2581 aatattcact caggctgtgt tcaaccaaat ccggctgaac tcttatgcag gatgcaacat
     2641 ctattatcct ttcgaataca acgtatctgt cttccttctc aaatatcttt gactttatca
     2701 aatagtattt tattttcctt atcaatacat aatcccaaat atactcggcc aagaccttgg
     2761 aaatctggtt gattccaatc ctgcatttta caaaaggctc gtcataccgg gcaccaggca
     2821 gttttttcat taaaaaacga accagttcct ccctgttgct gacagatgta atcctgaaag
     2881 gatacataat caaacctccc tgacttcctg ttctcattat atgctcatac aggttgttct
     2941 cgtgcatgct acaacttgta tatttgtatt cctccgatat cctcaagtaa atcattttta
     3001 agttccaaga tattgacacc ttgttcgagc aaaaattcct tcaccagacg accttccttg
     3061 tggtattcaa gacttccggt aagcaaaaga cccaaatctt caatgaatcc gctgcaaccg
     3121 ccaataaatc catggtcata tccctttaga attatatcgt ctccgttaaa ccaaaagcac
     3181 cggtctccca tctcttttgc ttttttctct atgcctttgt cggtagtcag aatgaagttt
     3241 tttacatata ttgaggaaca acctgcataa ccctgccgaa cattaacaaa atcatatcct
     3301 ttgtcagtaa aaacttttct cacaatacaa tcggtatatt ttgtattatg atatatattt
     3361 cttcccactt tcagcacgtt atatgcaata tttcccggat attttggcaa aagttgtgtg
     3421 ccgccttcta tcagctcaat tccagtatca tatagagcat ccaaagttct ttgattaatg
     3481 ccttttgcgt atacaagcac cttgttgtca atcttcgcaa gctgcatgtc agcatgacag
     3541 cttacagcct catggagatt tggatgcgga taaacaagaa ttacattgcc atattttttt
     3601 aattcttcaa ttattttttc atcagcctga caacttaaaa caaagtataa attcatgtaa
     3661 acaacctcat aaaaaagttt tttcagtgaa taataacatg ggaatgttaa attttcaaca
     3721 atgaacaaaa atgtattaaa taaattcaac aagggaaaca taagaacggt ggcataactt
     3781 atgtcacaga aaggagctga atcaaatggc cagaacaggt tttgatagat tcgcattaat
     3841 actggttata atcggtgcaa taaactggct gtcagtgggg ctgttccgat ttgacatcgt
     3901 tgcagctcta tttggaggtg ccacaaacct tgtaagcagg attatatact cagtagttgg
     3961 tattgcagga atctattcaa tcagtctttt gttcaggcac agtcctgtta acgttaggga
     4021 ataattaccc tgaataataa taatatatga gataataata taattgccgg tagtaaaccg
     4081 gtataaaaaa tggaggtatt gaaaatggca agaagcagaa acaagactac ctttgacaag
     4141 ctgaaatacg agattgccaa tgaaaccgga attaatctta aggaaggtta caatggcgat
     4201 ttgacttcca gagaagctgg tacaatcggc ggaaacattg ttcgtaaagt ttttgaaagc
     4261 tataccggaa acaattatta tacaggtaaa cctcaataag gtttgcctct aaccaaaagg
     4321 gaccagagag ttttctctgg ttcctttttt gctttgtatt ggttatttaa tttcaccttt
     4381 aatcatattt attacaaagg gatgtgatat gatgataaga atcattgttg tttttgcagt
     4441 aaccaccttg tttgtcttct ttcctgaaat cttccccagg tgcgaatact gtagaaaaat
     4501 aaaactaaga aagtgttttc agtttcataa aagcgtaagc cttaagttga cttacaaagg
     4561 caatctgtct ctgtgcaaaa aatgctgcaa gaaatataat tttacatccc tggacaaatt
     4621 caggaaacac atgcgcgtgg aaaaaagaat agaatacaca gtcaggtaca acctgtaact
     4681 actatgtatc ccctggattt gccaatccca aactaatcaa aagtgcctta tcaatc
//
LOCUS       DAONVY010000123         4732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13022_length_4732_cov_3.536027, whole genome shotgun sequence.
ACCESSION   DAONVY010000123 DAONVY010000000
VERSION     DAONVY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13022_length_4732_cov_3.536027"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PLI11_04470"
     CDS             <1..1504
                     /locus_tag="PLI11_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ52151.1"
                     /translation="DDIYMPHYVFTADQNGWPGDWEGRTILALVSLAKVLKEEPKYLD
                     GIIENLKDHLNEKGYLGHVLPKGTADEQQLSGHSWLLRGLCEYYRYKKDESVLETIKG
                     IVKNLFLPLRKNYETYPILPEQRVYEGHESGSVAGMVNNWYISTDTGCAFIPLDGISD
                     AYELMLDVDKDLARELRYMLGFMIDKFMTIPFTEICVQTHATLTGLRGMMRMFKHMRN
                     HVYLSFVKDTFDLYINEGMTANFANYNWFGRPEWTEPCAIIDSFMLACQLYKETAEQR
                     YADYANYILYNGIYRSQRENGGFGTDNCAGPEDDYVIPTNYEAYWCCTMRGGEGLFAV
                     SQYSLLERGKDIVMIYPVSGIYQVKGITMHIRTDYPKSGNISITIFENNSDKNVTMEF
                     MMFRTYVDVKFLYNFKQRKIIITKDKNEYLIDFKLPIYYKLAEGKHNKKDKKLLMRGP
                     AILGSLSPVSVPNELSNIVVEEFIPLYDTSFTRPRNRVEKDKIYILFDGQ"
     gene            complement(1498..2034)
                     /locus_tag="PLI11_04475"
     CDS             complement(1498..2034)
                     /locus_tag="PLI11_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52152.1"
                     /translation="MKLKKQLFGYSRKQTRKIIDDYEKRIDTLQDDNLYLKEKLEEVK
                     FMYEEEKRFSRDKIALKTIDVMVKANEEYANYLEEVSKEQGKLYEKFYELVDMIGVFQ
                     IRLKEMETLRNRIADANEMTREALKDFIKTTQSFDEKTGRDEQSGEKIGFAEFLVNHA
                     GEGSVVELLQLYVKLYNH"
     gene            complement(2081..2431)
                     /locus_tag="PLI11_04480"
     CDS             complement(2081..2431)
                     /locus_tag="PLI11_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52153.1"
                     /translation="MDIDTITKKVEELFALTKKISDSLDEEETDIDGIETLVANRQAI
                     IDEFVVHNVKFDTSLQEELKKIYEMDKENERKIKLILELYAKSVRNAANAKKQISYYP
                     KATNQEGLFIDEKS"
     gene            complement(2433..2834)
                     /gene="fliS"
                     /locus_tag="PLI11_04485"
     CDS             complement(2433..2834)
                     /gene="fliS"
                     /locus_tag="PLI11_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPZ52154.1"
                     /translation="MAVNPYQKYKTQAIMTASPGDLTLMLYDGCIKQMNLALLHIEEN
                     NHELKNNAIQKAEAILTELMSTLDMSYELSNNLFQIYEYVYRLLILANISNDTEKLNE
                     AINRITEIRDAWEEAIKIERKVAYDNTGDGA"
     gene            complement(2847..4367)
                     /gene="fliD"
                     /locus_tag="PLI11_04490"
     CDS             complement(2847..4367)
                     /gene="fliD"
                     /locus_tag="PLI11_04490"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPZ52155.1"
                     /translation="MSLNSINGLSSRLRLTGMASGLDTDSIVNDLMRIEQLKVDKVYR
                     QKVLAEWRREAVTNVRNKLRTFRDTYSSALSSLNMNTSSVYKKFNVEMAANPYVKITA
                     NSSAREGTYRIDKITRLAETAKLSANLSVDSIPGMYATLESVEGLEFHEEPMDPENGE
                     EVRKTLSFTINDETFTFESTDTIKHMLDTVNRSDAGVNMHFSELTMSLHIETKNTGED
                     AVIDFKNVEGSLFGLGDAVVNGEDAFVTINGVDVKKSSNSFTIDGITYNLTGKTNAPV
                     DFVVSRDVQHTVDKIKEFVQKYNELVEELNGLLSEKKNYDFAPLTEAQKKEMEAKDIE
                     EWEAKAKSGLLKNDPALSKLVNSLRSSIYQKVEGIGLALSDIGITTGSYLEKGKLHVD
                     ETKLAKYIKERPDDVMNLFMQSSPSGAELSTSEKYAQSGFFARMTSTFNTYYDSLQID
                     RMDRGIREYEDKISDLEIKLYEIQERYYRQFARLEAALSNMYSQQSWLYAQFNQGQ"
     gene            4533..>4732
                     /locus_tag="PLI11_04495"
     CDS             4533..>4732
                     /locus_tag="PLI11_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52156.1"
                     /translation="MKRRILVILLAGLVFTTNMSVIGERKIEELGDGYYRYVNDFNAS
                     KQFIINDTLLAFNNENNFQFKDG"
BASE COUNT         1341 a         1046 c          869 g         1476 t
ORIGIN      
        1 tgacgatata tacatgcctc attatgtctt taccgcagac cagaacggct ggcctggaga
       61 ctgggaagga agaaccatac ttgcccttgt ttcccttgcc aaggttttga aagaagagcc
      121 aaaatacttg gacggcataa ttgaaaattt gaaagaccat cttaacgaaa aaggttatct
      181 tggtcatgtc cttccaaaag gcactgcaga tgaacagcag ctgtctggcc acagctggct
      241 gctaagaggt ctgtgcgaat attacagata caaaaaagac gaaagcgttc tggagacaat
      301 taaagggatt gtgaagaacc tttttcttcc attaagaaag aactatgaaa cctatcctat
      361 acttcctgaa caaagagtat atgaaggaca cgaatcaggc agcgttgcag gcatggtgaa
      421 caattggtac atatccaccg acacagggtg cgcttttatt cctcttgacg gtatcagcga
      481 tgcttatgaa cttatgctgg atgtggacaa agacctggca agagaattaa gatacatgct
      541 gggtttcatg atagataaat tcatgaccat cccctttacg gaaatttgcg tccaaaccca
      601 tgccaccctc actggattaa gaggcatgat gaggatgttc aaacacatga ggaatcatgt
      661 ttatctttcc tttgtaaaag atacttttga tttgtatatc aacgaaggca tgaccgcaaa
      721 ttttgcaaac tacaattggt ttggaagacc tgaatggacg gagccttgtg caataataga
      781 ctcgttcatg cttgcatgcc agctgtataa ggaaactgcg gaacaaagat atgcagatta
      841 cgcaaattac atactctaca atggaatata tagatctcaa agagaaaacg gaggattcgg
      901 caccgacaac tgcgcaggac ccgaggatga ttacgttatc ccaaccaact acgaggcata
      961 ctggtgctgc acgatgcgag gaggagaagg cctttttgca gtctcgcagt acagcctttt
     1021 ggaaagagga aaggatattg tcatgatcta ccctgtcagc ggcatttacc aggttaaggg
     1081 cataacaatg cacatacgta cggactatcc caaatcggga aacatctcaa ttaccatatt
     1141 tgagaacaat agtgacaaga acgtcactat ggaattcatg atgttccgaa cgtatgttga
     1201 tgtaaaattc ctgtacaatt tcaaacagag aaaaattatc ataaccaaag acaagaacga
     1261 gtatctgatt gacttcaaac tccctattta ctacaaactc gcagaaggca agcataacaa
     1321 gaaggataag aagctcctta tgcgcggccc tgcaattctg ggaagtctga gtccggtgtc
     1381 cgttcctaat gaattgagca acattgtagt cgaggaattc atcccactct acgacacttc
     1441 atttacaaga cctagaaaca gagtggaaaa agacaaaata tatatactgt ttgacggtca
     1501 atgattgtat aatttgacgt agagttgcaa caattcaacg acactgcctt ccccggcatg
     1561 atttacaagg aactctgcaa atccaatttt ctcaccactt tgttcgtcac gcccggtctt
     1621 ttcatcaaaa gactgggttg tttttataaa atccttcaaa gcttccctgg tcatttcatt
     1681 agcatcagca attctatttc ttagtgtctc catttcctta agtcgaatct gaaatacccc
     1741 tatcatatct acaagttcat aaaacttttc ataaagcttt ccctgctcct tgctaacctc
     1801 ttccagataa tttgcatatt cctcatttgc cttaaccata acatcaatag tctttaaagc
     1861 aattttatcc cgggagaaac gtttttcctc ctcgtacatg aacttgactt cctccagctt
     1921 ctccttgaga tacaaattgt catcctgaag tgtgtctatt cttttctcgt agtcatcgat
     1981 tatctttctt gtttgctttc tactgtaacc aaaaagctgc tttttcaact tcataaaaac
     2041 ttcccctttt agacatggaa aaaggattgt tatcaacaat ctagcttttc tcgtcgataa
     2101 acaatccttc ctggttagtg gcttttggat aataggagat ttgcttttta gcattggcag
     2161 cgtttcttac gctttttgcg taaagctcga gaatcagttt gattttcctc tcattctcct
     2221 tatccatctc atagattttt tttaattcct cctgaagtga ggtgtcaaac ttaacattgt
     2281 gaacaacaaa ctcatctata atagcctgcc tgtttgcaac aagcgtttca ataccatcaa
     2341 tgtccgtctc ctcttcatca aggctatcgg atattttttt tgtaagtgcg aacaactctt
     2401 caaccttttt cgtaattgta tcaatatcca tactatgctc catcgccggt gttgtcgtat
     2461 gcaacttttc tctcaatctt tatagcttct tcccatgcat cccttatctc ggttatcctg
     2521 ttgatagcct cgttgagctt ctccgtgtcg tttgatatgt ttgcaagtat gagcaatctg
     2581 tacacatact catatatctg gaacagattg ttcgataact catagctcat atcaagggta
     2641 gacataagtt ccgtcaagat tgcctccgcc ttctggatgg cattgttctt aagctcatgg
     2701 ttgttctctt caatgtgcaa gagcgccaaa ttcatctgct tgatgcatcc gtcataaagc
     2761 atcagggtaa gatcccctgg cgatgcagtc ataatcgctt gcgttttata cttctgataa
     2821 ggatttaccg ccattttcac ctctccttac tgtccttgat taaactgtgc gtaaagccag
     2881 gactgctggg aatacatatt ggacaaagcc gcttcaaggc gggcaaactg cctgtagtat
     2941 ctctcctgga tttcatacaa ttttatttca agatcactaa tcttatcctc gtactccctt
     3001 attcctctgt ccatcctgtc aatctgaagg ctgtcatagt atgtattgaa agtagaggtc
     3061 attcttgcaa agaatccact ttgcgcatac ttctcactgg tagacagttc tgctcctgaa
     3121 ggagaagact gcatgaaaag attcatgaca tcatcgggtc tttctttaat gtactttgca
     3181 agcttggtct catccacatg aagttttccc ttctccaggt aacttccagt ggttatgcca
     3241 atatcgctta gggcaagacc tataccctcc accttttgat atatggaaga gcgcaatgag
     3301 ttaaccaatt tggaaagagc gggatcgttc ttaagaagac cactttttgc cttggcctcc
     3361 cactcctcaa tatcctttgc ttccatctcc ttcttttgag cctcggtcaa tggagcaaag
     3421 tcataattct ttttctcact tagaagacca ttaagctctt ctacaagctc attgtatttc
     3481 tgtacaaatt cctttatctt gtcaacagta tgctgcacat ccctgcttac aacaaaatcc
     3541 acaggggcgt ttgttttgcc tgtaaggttg tatgttattc catcaattgt gaaactgttg
     3601 gaactctttt taacatccac accgttgatg gtaacgaatg cgtcttctcc gttaacaaca
     3661 gcatcaccca gaccaaacag actgccttca acattcttga aatcaattac agcatcctcg
     3721 cctgtgttct tggtctcaat atgcaggctc attgtaagtt cgctgaaatg catgtttacc
     3781 cctgcatcgc ttctgttaac tgtatccagc atatgtttta ttgtatccgt tgattcaaag
     3841 gtaaatgtct catcatttat tgtaaatgac agtgttttcc taacctcttc tccgttttca
     3901 ggatccattg gctcttcatg aaattccaaa ccttctacag attccaaggt ggcatacata
     3961 ccaggaatac tgtcaacaga aagatttgca gacagctttg cagtctctgc aagtcttgtg
     4021 attttatcta tcctgtatgt accttccctt gctgaactgt tagcggttat ctttacataa
     4081 gggtttgcag ccatctcgac attgaacttc ttgtatacgg aggaagtgtt catgttcaag
     4141 ctactgagag cactagaata agtgtctctg aacgttctca gcttgttcct gacatttgtt
     4201 acagcttccc ttctccactc tgcaagaact ttctgtctat ataccttgtc aaccttaagt
     4261 tgttctattc tcatcaaatc attaacgatg gaatcagtat caagtcctga tgccattccg
     4321 gtcaatctga ggcgtgatga caacccattt attgagttta acgacataac attacctcct
     4381 ttgttttcta cactaatatt atcgatatta agggagaaaa ctttagcaat tcagaccaca
     4441 tattaattta tccgtaccca gaagttgtat ttgtgctgtt aatggtatag aatagacata
     4501 aatttactga tggtagaggg gtgtaatgca tcttgaaacg aagaatattg gtaatcttac
     4561 ttgcaggcct tgtattcaca acgaacatgt ctgtaatagg cgagagaaag atagaagagc
     4621 tgggagacgg ctattacaga tacgtgaatg actttaatgc ttcaaagcag ttcataatca
     4681 acgatacact gcttgctttt aacaatgaaa acaatttcca atttaaagat gg
//
LOCUS       DAONVY010000124         4728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13040_length_4728_cov_3.493259, whole genome shotgun sequence.
ACCESSION   DAONVY010000124 DAONVY010000000
VERSION     DAONVY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13040_length_4728_cov_3.493259"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLI11_04500"
     CDS             <1..618
                     /locus_tag="PLI11_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPZ52157.1"
                     /translation="PERIEKSKQIVDLALELGTNIITTHIGVIPEDKNDDTYKVMLEA
                     CAELAEYANSVGAFFAIETGPETAVVLREFLDQLPGKGVAVNFDPANLVMCVDGVPER
                     DVKILGDYIVHTHAKDGIMVKGKDPAKVQEVLDACGYGDTDFTNLPWIELPLGCGHVD
                     FPKYLKALKETGFDGFLTIERETGPDPFKDIEMAVKFLKEQMANL"
     gene            758..1579
                     /locus_tag="PLI11_04505"
     CDS             758..1579
                     /locus_tag="PLI11_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52158.1"
                     /translation="MKIKRIALLTLAAIICFSMFMVSASTKGYVTDGLVGFFDGSNNQ
                     GNKHDKQSTTWKDLSGTGNDITVKLDDKCKWNDNGYFNDSTRIYLPQALTDLINSNEF
                     TVEIVLNTFKSKGLHYNPIMNCDNDMFSLFRRIGDEVMQFKNNGNPRPTSETGVALDY
                     LSERVTLTITYKVGGKSTMYINGEKIDAQNAGVAVGADKMFFGHDDATRNFSTNYEYL
                     RFYNRELSADEVSKNYNEDLKASNPTTGVTTTFFYMAVTLGLAGTALKGMKKKED"
     gene            1726..>4728
                     /gene="carB"
                     /locus_tag="PLI11_04510"
     CDS             1726..>4728
                     /gene="carB"
                     /locus_tag="PLI11_04510"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPZ52159.1"
                     /translation="MTKNDNNKIMIIGSGPIIIGQACEFDYSGTQACKALKMLGYEVV
                     LVNSNPATIMTDPETADATYIEPLNVETLEKIIEKERPFALLPSLGGQTGLNLSQELA
                     KKGILEKYGVKLLGVQLDAIERGEDRIAFKETMVKLNIDMPRSAPAYSVEEAEKIAGE
                     MGYPVVVRPAYTMGGTGGGIVYNIEELRTVVYRGIAASMVGQVLIEECVLGWEELELE
                     VIRDKDGRKVTVCYIENVDPMGVHTGDSFCVAPTITVSEELKKRLQDYAYRIVDAVGV
                     IGGCNVQFAHDPVTDRVVIIEINPRTSRSSALASKATGFPIAYVSALLAAGITLPEIP
                     YWKKGTLDKYIPEADYIVVKFARWAFEKFKDAEDKLGTQMHAVGEVMSIGKSYKEAFQ
                     KAIRSLETGRSGLGFAKNYNQLDKDELFKLLAHPTSERQYVLYEAFRKGATIEEIHEL
                     TKINRWFLEQMKELVEEEEELLKYKGKMPPVELLERAKINGFSDKYLAQILEIDEREL
                     WKYREEKGIIQRWEGVNVSGIETESYYYYSTYEKGMNMSVRNENSIMVLGGGPNRIGQ
                     GIEFDYCCVHAAKALRELGFKTIMVNCNPETVSTDYDTSDKLYFEPLTAEDIISIYNH
                     EKPLGIIVQFGGQTPLNIADELQKRGARILGTPPAVIDAAEDRDLFNKMMNKLQIPMP
                     ESGMAINEKEAIEIAERIGYPVMVRPSFVLGGRGMEIIHDSDMLKTYMAQIEEVAPDR
                     PVLIDKFLDNALECEADAISDGENVFVPAVMEHIEQAGVHSGDSACVIPSKTISQKHL
                     DTIYSYTAKIAIEMGVVGLMNIQYAIYNDEVYVLEANPRASRTVPLVSKVCAINMAQI
                     ATNLMVASLIDEERKIDLTKLKRKDIPYYGVKEAVLPFNMFPEVDPVLGPEMRSTGEV
                     LGLADTFGSAFFKSQVGANGTLPVGGNVLISVNENDRQGILEVARGFKKLGFHIIATI
                     GTSTFLSEHGIETEVVKKLYEGRPNITDL"
BASE COUNT         1526 a          868 c         1155 g         1179 t
ORIGIN      
        1 cctgagagaa ttgaaaaatc aaagcaaatc gttgatcttg cattggagct tggaacaaat
       61 attatcacaa ctcatatcgg tgttattcca gaggacaaga acgatgacac atacaaggta
      121 atgcttgagg cctgtgctga attggctgaa tatgcaaaca gcgtgggtgc attctttgcg
      181 atagagaccg gtcctgaaac cgcagttgtt ttaagggaat tccttgacca gcttcctggc
      241 aagggagtgg ctgttaactt tgaccctgca aacctggtaa tgtgtgtgga tggagtacct
      301 gaaagggatg taaagatatt gggtgactac attgttcaca ctcatgccaa agacggaata
      361 atggttaaag gcaaggatcc tgcaaaggtt caagaagtgc ttgatgcctg cggttatgga
      421 gatactgatt ttaccaatct tccatggatt gaattgcctc ttggatgcgg tcatgtagac
      481 ttccccaagt atctcaaagc tcttaaggaa acaggatttg acggtttcct gactatagaa
      541 agagaaacag gtcctgatcc attcaaggat attgagatgg ctgtcaagtt cctgaaggag
      601 caaatggcaa acctttaaga tttgaactta ttaactcaga gactataagt cttcgagtta
      661 tttttaaatt tattgaacat atatttacta aaatgcatct ttgtgatata ttagacaaaa
      721 gtaaagagtg cctaatataa tgacaggagg tagtattatg aaaatcaaaa ggattgcatt
      781 attgacacta gctgctatca tttgtttttc aatgtttatg gtatcggcat caactaaagg
      841 ttatgtaacc gatggtcttg ttggtttctt tgatggaagc aacaaccagg gaaacaaaca
      901 tgacaagcaa agtacaacct ggaaggatct gagcggaact ggcaatgaca ttaccgtcaa
      961 gttggatgac aaatgcaagt ggaatgacaa tggttacttc aatgattcaa ccaggattta
     1021 tctgccacaa gctctgacgg atttgattaa tagcaacgag tttacagtgg aaattgttct
     1081 taataccttt aaatcaaaag gtttgcacta caatccaatc atgaactgtg acaatgacat
     1141 gttctctttg ttccgtagaa taggagatga agttatgcag ttcaagaaca acggaaatcc
     1201 aagacctact tcagagacag gcgtggctct tgattatctt tccgaaagag tgacactgac
     1261 catcacctac aaggtgggtg gcaagtcaac catgtacata aacggtgaga agatagatgc
     1321 acaaaatgcg ggtgtagccg ttggtgcaga caaaatgttc tttggacacg atgatgcgac
     1381 aagaaacttc agcacaaatt atgaatactt gcgtttttat aacagagaat tgtcagcaga
     1441 tgaggtttca aagaactaca acgaagacct gaaagcatca aacccaacca ctggtgtaac
     1501 tacaaccttc ttctacatgg cagtgactct cggtcttgca ggcaccgcat taaagggtat
     1561 gaagaaaaaa gaggactaag ataattgaat aatcagcaaa ggagctgtcg caactgcggc
     1621 agcttttttc gtgtttaaca catccgattt aatcgtgaca gtatttttca tctatgatat
     1681 aatttcatga atcgggatac cgataatctt taggaggatt cttttatgac aaaaaacgac
     1741 aacaacaaaa tcatgataat agggtcggga cccatcataa ttggacaggc ttgtgagttt
     1801 gactactcag gaacccaggc atgcaaggcg ttgaaaatgc ttggttatga agttgttttg
     1861 gtaaattcaa atcctgctac cattatgacg gatccggaaa cagcagatgc aacgtacatt
     1921 gaaccgctta atgtagaaac attggaaaag ataattgaga aagaaaggcc atttgctttg
     1981 ctgccaagtt tgggaggcca aaccggactg aatttaagcc aggaactggc caagaaggga
     2041 atccttgaaa agtatggcgt caaacttctg ggcgtgcagc tggatgccat tgaacgaggc
     2101 gaggacagaa tagcctttaa ggaaacaatg gtaaagttga atattgacat gccgagaagc
     2161 gcacctgcgt actcagtgga ggaagctgaa aagattgcag gggaaatggg atatcctgtt
     2221 gttgttaggc ctgcttacac catgggcggt acaggcggag gaatagttta caacatagaa
     2281 gaattaagaa cggtagtata cagaggcata gcagcatcga tggtaggaca ggtgctgatt
     2341 gaagaatgtg tgcttggatg ggaagaactg gagctagaag taataaggga caaggatggc
     2401 cgcaaagtta ccgtctgcta tatagaaaat gtagatccta tgggtgtgca tacaggtgac
     2461 tctttttgcg tggctccaac aataaccgtg tcggaggaac taaagaagag gcttcaggac
     2521 tatgcataca gaatagttga cgcagtaggc gttattggag gctgcaatgt gcagtttgcc
     2581 cacgaccctg taactgatag ggttgtaata attgaaataa atccaagaac ttcaaggtca
     2641 tccgctcttg catcaaaggc cactggattt cctattgctt acgtttcagc actgcttgca
     2701 gcaggtataa cactccccga gattccatac tggaagaagg gcactctaga caagtatata
     2761 cctgaagcag attacattgt tgtcaagttt gcaagatggg catttgaaaa atttaaggat
     2821 gcagaggaca agctgggcac ccagatgcat gcggtgggag aagtcatgtc cattggcaaa
     2881 tcctacaagg aagcctttca aaaagccata aggtctttgg aaacaggaag gagcggtctt
     2941 ggatttgcaa aaaactacaa ccagcttgac aaggatgagc tgttcaaact gctagcccat
     3001 cccaccagtg aaagacagta tgtcttgtat gaagcgttca gaaaaggtgc caccattgaa
     3061 gaaatccatg aactaacaaa gataaacaga tggttccttg agcagatgaa ggaacttgtg
     3121 gaggaagagg aggaacttct taaatacaag gggaaaatgc ctcctgttga acttttggaa
     3181 agagcgaaaa taaacggttt ttccgacaag tatcttgccc aaatacttga aattgatgag
     3241 agggaactct ggaagtatag ggaagaaaaa ggcataattc aaagatggga aggcgtcaat
     3301 gtaagtggaa ttgaaacaga aagctactat tactactcca catacgaaaa aggcatgaat
     3361 atgtccgtaa gaaacgaaaa ctcaatcatg gtacttggcg gaggacccaa cagaataggt
     3421 cagggtatag agtttgacta ctgctgcgtg catgcggcaa aagcactcag ggaactaggg
     3481 tttaagacaa taatggtaaa ctgcaaccct gagacggttt ctactgatta tgacacctcc
     3541 gacaaactat atttcgaacc attaaccgca gaagatataa taagcatata taaccatgaa
     3601 aagccgcttg gcattattgt tcagtttgga gggcaaactc ctctcaacat tgccgacgag
     3661 ctgcaaaaaa gaggcgcaag aatccttgga actcctccag cagtaattga tgcggcggaa
     3721 gacagggatt tgttcaacaa aatgatgaac aagctgcaga tacccatgcc tgagtccgga
     3781 atggccataa atgaaaaaga agctattgaa attgctgaaa ggataggcta tccagtaatg
     3841 gtacgtccat cctttgtact gggtggcagg ggtatggaaa taattcacga tagcgacatg
     3901 ttaaaaacat acatggctca aatagaggaa gtggcacctg acagacctgt tctcatagac
     3961 aagtttcttg acaatgcttt ggaatgtgag gctgacgcaa tatctgatgg agagaatgtg
     4021 ttcgtgcccg ctgtcatgga gcatattgag caggcaggcg tccattcggg agactctgct
     4081 tgtgtcattc cgtcaaaaac aatatcacaa aaacatcttg acaccattta cagctacacg
     4141 gcgaaaatag caatagagat gggtgttgtc ggccttatga acatccagta tgcgatatat
     4201 aatgatgagg tgtatgttct tgaggcaaac ccaagagcat ccaggacagt gcctctggtt
     4261 tcaaaagtat gtgcaataaa catggcgcag attgcaacga atcttatggt tgccagcctt
     4321 attgacgagg aaagaaagat agacttaacc aaacttaaga ggaaggatat cccatactat
     4381 ggagtgaagg aagcggtgct gccgtttaac atgttccctg aggtggaccc ggtactaggc
     4441 cctgagatga ggtccacagg agaggttctt ggccttgcgg acacctttgg cagtgcattt
     4501 tttaaatccc aggtgggtgc aaacggcact ctgcctgttg ggggcaatgt gctgattagt
     4561 gtaaacgaga atgacaggca aggtattctt gaagttgcaa gaggctttaa gaaactcggt
     4621 tttcatataa tcgcaacaat cggaactagc acgtttctaa gcgagcacgg gatcgagacc
     4681 gaggttgtca agaaattgta cgaaggaagg ccgaatataa cggacttg
//
LOCUS       DAONVY010000125         4711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13100_length_4711_cov_4.317225, whole genome shotgun sequence.
ACCESSION   DAONVY010000125 DAONVY010000000
VERSION     DAONVY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13100_length_4711_cov_4.317225"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..203
                     /locus_tag="PLI11_04515"
     tRNA            128..203
                     /locus_tag="PLI11_04515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:161..163,aa:Thr,seq:ggt)
     gene            341..1441
                     /locus_tag="PLI11_04520"
     CDS             341..1441
                     /locus_tag="PLI11_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52160.1"
                     /translation="MKRVLCIFIFLFIFTASFVHAEEMEVFDKVDFEDLQTGSVDGSD
                     FAVLWDFSNPAGNTPLIRDEGGNKYLRMDGYSQIISPHIMESDEYVFETKIRLTYGYR
                     WVGFFVRAANPVLRTNPYHSEYSESNPIGMHIHFYESDWYYDAEQKGESNMGGSGIGI
                     FPRKGHVRVAIKTYIPNALNMGIVYHDFPYPEDTNPKEFVKYTVRDNGTDTIEIYIND
                     HFITKITFSEPVVYEEDPHDENEYYSVASMTDLEGNVFDLYDQEDRKIEILDNARIAV
                     FSHRIAFASRTQSFDIDDIVFYHKASQNTLQPTQSPDRTNENPVNTTPDNTVVNDNQL
                     NYPAILITTSSAIVTVIFISYMIILFRKTYRR"
     gene            1447..3438
                     /locus_tag="PLI11_04525"
     CDS             1447..3438
                     /locus_tag="PLI11_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52161.1"
                     /translation="MQKYINIILSFLLFATILPGRAVSHASDPSTWTAVDGLGRKLPD
                     QSEVGSVRKDRYVGIFYWTWHDFFSVWEPRNITKILEEHPEAANDFSHAAWGNMPSGS
                     PYFWNEPMFGYYSSKDVYVLRKHAELLADALIDVIVFDCTNDTNLFLDAANVLFKVFE
                     QAKEEGVNVPKFAFMLNINPTVMMENNRIELRELYEKIYKLEKYKDLWFYWEGKPIVF
                     STDKCLDASDPLEKEILDFFTFRRMDDMFDTKDTKYEDKMWGWLSVYPQTKYGVRDDG
                     TVEQMAVGIAQNYSDEHRLTAMNDPRGGVHGRSFAANDYSYSYTYKGKTITVDSNTEN
                     KILYGLNFQQQWDYALSVDPDFIFVTGWNEWIAGRWEKWIDVDNAFPDQYNDEYSRDI
                     EPSNGFLKDHYYYQLVANVRRFKGVNDTAIHGEMKTIDIHGDISQWNDVKAEYVHYTG
                     NTPKRRSHKGYGRTKYTSDTMRNDIVKAKVAYDEKHAYFYVETVDALTSPSDNAWMRL
                     FLDVDSDDMLDWEGFEYVVNRLSPSEKKAVLERSLGGWNWEKVGDVEYSVKGNVLQLA
                     LPRNMIGMEKAVNFNFKWVDNTQEDGDIMDFYKSGDSAPIGRFSFVFRSTPLDSSVER
                     QGLTLPWYTYLSMIVAVISVLMAVGFRLFVVKRKLKSSS"
     gene            complement(3422..4168)
                     /gene="hisA"
                     /locus_tag="PLI11_04530"
     CDS             complement(3422..4168)
                     /gene="hisA"
                     /locus_tag="PLI11_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HPZ52162.1"
                     /translation="MKFRPCIDIHEGKVKQIVGSSISDDSVLENFVSTKDAEYYANLF
                     KKDSLKGGHVIKLCKSKETEEEAKRALKAFPLGMQIGGGIDSDNCLNYLSLGASHVIV
                     TSYIFHNGGIDMDNLSKLVKLCGKERLVLDLSCIEKDNNYHIATDRWTKITNTILNEE
                     TLEYFSFFCDEYLIHAISKEGKKAGIDEKLVEILSSSPIPVTYAGGISSYDDIDRIKL
                     IGKNKVDYTVGSALDIFGGYLEYDKIKKMI"
     gene            4268..>4711
                     /locus_tag="PLI11_04535"
     CDS             4268..>4711
                     /locus_tag="PLI11_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52163.1"
                     /translation="MVSIREVSKTYGKKQVPAVDSLNLEIREGEIFGFIGPNGAGKTT
                     TIKMLTGILPPDKGSIVIDGVDLVKNPVEAKMKIGYVPDNPNIYERLTGMEYLEFIGD
                     VYGVPYAERRERINKYMKMFEMEFSFNDQIRSYSHGMRQKLLITGA"
BASE COUNT         1466 a          785 c         1048 g         1412 t
ORIGIN      
        1 cttcggaaga agtaaatgca actgtatctt tgcatttaaa aattcaaatg accaaaagga
       61 taaattttta aaaaaaaggc tggacaactg aaatattatg tgttaaaata tatttcggtt
      121 tcagccagcc ctcatagctc agtaggcaga gcgcatccat ggtaaggatg aggtcaccag
      181 ttcgattctg gttgagggct ccacaaaaaa gcacagttta aaagactgtg cttttttttt
      241 gcttgtatat atttccggta gatagtataa ttcaagaaac ttggtcaata cacatgggaa
      301 gaaagacaaa atatctgacg taaactttgg aggttcttga atgaaaagag ttttatgtat
      361 ctttatattc ctgtttatat ttacagcatc ctttgtacat gcagaagaaa tggaagtgtt
      421 tgacaaggtt gactttgaag atttgcaaac tggtagtgtt gatggaagcg attttgcagt
      481 tctttgggat ttctcaaatc cagctggtaa cactccatta attagggatg agggaggcaa
      541 caagtattta aggatggatg ggtattctca aataatatct cctcatatta tggaatctga
      601 tgaatatgtg tttgagacaa agatacgcct cacgtatgga tacaggtggg taggtttttt
      661 tgtaagagct gccaatcctg tcttaagaac caatccatat cattccgaat acagtgaatc
      721 aaaccccata ggcatgcata ttcattttta cgaatcggat tggtattatg atgcggagca
      781 aaaaggcgag tccaatatgg ggggaagtgg cattggcata ttcccaagaa aaggccatgt
      841 aagagtggcg ataaaaacct acatacccaa tgcgttgaac atgggaattg tctatcatga
      901 ttttccttat ccagaggaca caaatcccaa agaatttgtc aagtatactg taagggataa
      961 cggtacggat acaatagaga tctacataaa cgatcacttc ataacaaaga ttactttttc
     1021 agaaccggta gtatatgaag aggatcctca tgatgagaat gaatattatt ctgttgcttc
     1081 aatgactgat ttggaaggca atgttttcga tttgtatgat caggaggata gaaagataga
     1141 aatcctagac aatgcaagga ttgccgtctt ctcccacaga attgcgtttg caagcagaac
     1201 tcagagtttt gatattgatg acattgtttt ctatcataaa gcaagccaaa acacattaca
     1261 gccaactcaa tcaccagaca ggactaatga gaatcctgta aacacaacac ctgataatac
     1321 ggtggtcaac gacaatcagt tgaactatcc tgccatactg attaccactt catcagctat
     1381 tgtaactgta atatttattt catacatgat tatattattc agaaagactt acaggaggta
     1441 gggcaaatgc aaaaatacat aaacatcatt ttatcttttc ttttgtttgc aacgattctg
     1501 ccaggtcgtg ccgtttccca tgcaagtgat ccttctacat ggactgcagt agacggtctt
     1561 ggaaggaaac tgcccgatca aagcgaagtg ggcagtgttc gaaaagacag gtatgtaggt
     1621 attttttact ggacctggca tgactttttt tctgtgtggg agccaaggaa tattacaaag
     1681 atattggagg aacatccgga agctgcaaat gatttttctc atgctgcctg gggcaatatg
     1741 cccagtggtt ctccatattt ctggaatgaa cccatgtttg gatattacag cagcaaggat
     1801 gtttatgttc taagaaagca tgcggagctt ttggcagatg ccttgattga tgtgattgtg
     1861 ttcgactgca cgaatgatac caacttgttt cttgatgctg ccaatgtgct gttcaaggtg
     1921 tttgagcagg ctaaagagga aggtgtaaat gttcccaaat ttgcctttat gttaaatatt
     1981 aatcctacag tcatgatgga aaacaacaga atcgaactta gggaattgta cgaaaagatt
     2041 tacaaactgg agaaatacaa ggatctgtgg ttctactggg aaggaaaacc aatagtattt
     2101 tctactgaca aatgtttgga tgcctctgat cctttggaga aggaaatatt agatttcttt
     2161 acattcagaa gaatggatga catgtttgat accaaggata cgaagtatga ggacaagatg
     2221 tgggggtggc tatccgtata cccacagacc aagtatggtg tgagagatga tggaactgta
     2281 gaacagatgg ctgttggtat cgcccaaaac tatagtgatg aacatcgttt gacggcaatg
     2341 aatgatccaa gaggtggagt gcatggaaga agttttgctg caaacgacta ttcatactcc
     2401 tatacttaca aaggcaaaac cataactgtt gattctaata cggaaaacaa gattttgtat
     2461 ggtcttaatt ttcagcaaca gtgggattat gcattatctg ttgatccgga ttttattttt
     2521 gtgactggtt ggaacgagtg gattgcaggc agatgggaaa agtggattga tgttgacaat
     2581 gcctttcctg atcaatacaa cgatgaatac agcagggata ttgaaccttc aaatggattt
     2641 ctaaaggatc attactatta ccagctggta gcaaatgtta gaagattcaa gggagttaat
     2701 gatactgcca tacatggtga aatgaaaact attgatattc atggagacat atcccagtgg
     2761 aatgatgtaa aagcagaata tgtgcattat acagggaata ctccaaagag gaggagtcac
     2821 aagggatatg gaaggacaaa atacacctcc gacacgatgc gaaatgacat tgtgaaggca
     2881 aaagttgcat atgatgaaaa gcatgcatac ttttatgttg aaactgtaga cgctttgaca
     2941 agtccttccg ataacgcatg gatgagattg tttttggatg ttgactctga tgatatgttg
     3001 gattgggaag gctttgagta tgtagttaac agactgtctc cttctgaaaa aaaagctgtt
     3061 ctggaaagaa gtctgggagg ctggaattgg gagaaagtag gggatgtaga gtactcggta
     3121 aagggtaacg tgttgcagct tgctttacct aggaacatga taggcatgga gaaggcggtc
     3181 aactttaatt tcaaatgggt tgacaatact caggaagatg gcgacattat ggatttttac
     3241 aagtccggag attctgcacc tataggtaga ttttcatttg tattcagaag tacgcccctt
     3301 gacagcagcg tggaaagaca aggattgaca ttgccctggt atacttatct ttccatgatt
     3361 gtggcggtta taagtgttct gatggcagtt gggttcaggt tgtttgttgt taaaagaaag
     3421 ctcaaatcat cttcttgatt ttgtcgtatt ccaaatatcc cccaaaaatg tccagagcgc
     3481 tgcctactgt gtaatcgacc ttgtttttac ctatgagttt gattctgtca atatcgtcgt
     3541 aggaggatat gcctcctgca tatgtaacgg gaatagggga agaggacagt atttcaacga
     3601 gtttttcgtc gatacctgcc ttttttcctt cttttgatat tgcatgaata agatattcat
     3661 cacaaaagaa tgagaagtac tcaagtgttt cttcgtttag aattgtgttt gtaatctttg
     3721 tccatctatc tgttgctata tggtagttgt tgtccttttc gatacaacta agatcaagca
     3781 caagtctttc tttaccgcac aacttgacca atttgcttag attgtccatg tcaattccgc
     3841 cattgtggaa gatgtatgag gttacgataa catgagatgc acctaaagaa aggtaattga
     3901 ggcaattgtc tgaatctatg cctccaccta tttgcattcc caaaggaaat gccttcaagg
     3961 cccttttggc ttcctcttca gtctcttttg acttgcacag ttttataaca tggccacctt
     4021 ttaggctgtc ctttttaaaa agatttgcat aatactctgc atcttttgtt gaaacaaagt
     4081 tttccaatac actatcatca cttattgagc ttccgactat ctgctttact tttccttcat
     4141 gtatgtcaat acaaggccta aatttcatat ttgcctccat agtaaccttg atatgggtaa
     4201 taaatttatg gtatacttca gtattgtaac ataaacaaaa tgctgtggca aagaggagat
     4261 gggtttagtg gtatcaatca gggaagtcag caaaacttat ggaaaaaaac aagtccctgc
     4321 tgtggacagc cttaatcttg agattaggga aggagaaata tttggattta ttgggccaaa
     4381 tggtgcagga aagactacaa caataaagat gctgacaggc atactgccac ctgacaaagg
     4441 ctccattgta atagacggtg tggatttggt taagaatcct gttgaagcaa aaatgaaaat
     4501 aggttatgtg ccggataatc caaacatcta tgagagatta accggcatgg agtatttgga
     4561 gtttattgga gatgtttacg gcgttccata tgcagagaga agggaaagaa tcaacaagta
     4621 tatgaagatg ttcgaaatgg aattctcctt taacgaccag ataagaagct attctcatgg
     4681 tatgaggcag aaacttctca ttactggagc g
//
LOCUS       DAONVY010000126         4711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13101_length_4711_cov_2.972938, whole genome shotgun sequence.
ACCESSION   DAONVY010000126 DAONVY010000000
VERSION     DAONVY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13101_length_4711_cov_2.972938"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PLI11_04540"
     CDS             <1..142
                     /locus_tag="PLI11_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HPZ52164.1"
                     /translation="NKFYNSCSILKAEEEVMKARLNLVEASCICIKTGLNLLGIEEVE
                     KM"
     gene            158..1306
                     /locus_tag="PLI11_04545"
     CDS             158..1306
                     /locus_tag="PLI11_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52165.1"
                     /translation="MKKVLIFLIMVTLVFSMISCSDTGNGENKEPENKWEGDNMEDFI
                     AEGLEVSDKGAGIYPRVPYEERFVGLAYTTWFRPQIWSSSVWAYPTLGTYNSDDKEII
                     RQHAELIYDAGVDFVWVDWSNNLDFDMPMDRVLKDRTSRADFAMIERSTVYMFDVYSQ
                     MEKAPKISIFIGCPAPGQKEGVTDGRLTRKADQIYDWFVNNPEHPEYKDQLFYYNGKP
                     LLVVYVGTPSPWQSGVPEWDDDRFTVRYMTGYVTEQKGLRDPDTLESKYGYWSWEDRV
                     AQTFTVSDGYPEAMIVCASTRPQAEEGDPNYIPAVERRNGQTFKEQWARARLIGVRFA
                     MVGTWNEYVTGEQYSDEVSKDIEPNTVWGDMYLKLLKEEIAKFKGKNK"
     regulatory      1386..1492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1545..2240
                     /gene="thiT"
                     /locus_tag="PLI11_04550"
     CDS             1545..2240
                     /gene="thiT"
                     /locus_tag="PLI11_04550"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HPZ52166.1"
                     /translation="MNKNLFEFLTDGTEILKFDKIFENPVVFGIVVLVAIALVVFLVL
                     QYRKEKDAPKPKRKTDVKVLVTGALCISIAFVLSFIKLLDLPQGGALTLASGLPIILF
                     AYLYGPKHGLAAAFAYSLLQLLQGAYTVGIIQFLLDYPLAFTAFGLAGFYRKNIIPGI
                     IAGYMVRLMCHFVSGVVFFGEYAKELGYAVVPYSFMYNAVFLIPEMILCIIIVLIPQV
                     KRMIGNIRKELAV"
     gene            2274..2879
                     /locus_tag="PLI11_04555"
     CDS             2274..2879
                     /locus_tag="PLI11_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPZ52167.1"
                     /translation="MKRKKVIIALLIAVCLLGLLALIAINLYMVKYSRQYILDDESIK
                     DMDAEYIVVLGAKVYKDGRLSLILDDRVKASVKLFEEGRVPAIIMSGDAQREDYNEVD
                     PMKKRAIELGVPEECIITDTMGLSTFESMKRLKDEFGVDKVIICTQEYHMYRAIYNAR
                     KMGIEAYGYPAEKIDYANQLQRDIREAIARVKDFILVLFTT"
     gene            complement(2973..3686)
                     /locus_tag="PLI11_04560"
     CDS             complement(2973..3686)
                     /locus_tag="PLI11_04560"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HPZ52168.1"
                     /translation="MSALTLKIIACIAMLLDHIGYTLKITYSYRIIGRIAFPIYAFLL
                     TEGYHHTKDFKRYLKRLFLFALVSEIPFNLFSKGKLIDISSSKNIFFTLSLSLLLLYW
                     LDESKKIYEDKPRLILLSTILVSLFCLTAELINVDYGFYGILTVLCFRYMKHCRRKNL
                     ATITGHTVVIFSYILLSQNNSWAFVQLYALFALVPILLYNGKKGHNPTNRTMNKIIQY
                     AFYAYYPLHMLIIYLFTSL"
     gene            3813..3962
                     /locus_tag="PLI11_04565"
     CDS             3813..3962
                     /locus_tag="PLI11_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPZ52169.1"
                     /translation="MYECLCGYVYDPAEGDPENDIPAGTAFEDLPDDWVCPICGLDKS
                     AFSEV"
     gene            4035..>4711
                     /locus_tag="PLI11_04570"
     CDS             4035..>4711
                     /locus_tag="PLI11_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPZ52170.1"
                     /translation="MNICVSSYSFSRLVREGSLKPENVIYKAKEMGFYGIEFAGLWVP
                     EGMTALDYAKHVKDLAEDAGIKITGYSVGGNLLCNNQEEEIERLKGEVDVAHALGVNI
                     MRHDVAYEPMPGGYKSFENNLPLLIKGVRAVTEYAESLGVRTSIENHGFYTQDSHRVE
                     MLIDGVNSDNYGALLDLGNFLCVDEDPAKATGVLKNYVFSVHAKDFYFHDGDETPSSP
                     GWFMTRGG"
BASE COUNT         1402 a          777 c         1167 g         1365 t
ORIGIN      
        1 caataagttc tacaatagct gcagcatatt aaaggctgaa gaggaggtta tgaaggcaag
       61 gctcaatttg gtagaggctt cctgcatatg cattaaaacc ggactcaatt tattaggaat
      121 agaagaggtc gaaaaaatgt aactggggag gtgtcccgtg aaaaaggtac tgattttcct
      181 gattatggtt accttggttt tttccatgat atcctgttca gacacgggta atggcgagaa
      241 taaggaacca gaaaacaaat gggagggaga caacatggaa gattttattg cagaaggtct
      301 tgaggtatcg gacaagggag caggcattta tccaagagtg ccttatgaag aaagatttgt
      361 gggtcttgcc tacaccacat ggtttagacc acaaatatgg agttcaagtg tttgggcata
      421 tccgactctt gggacataca actccgatga caaggaaatc ataaggcagc acgcagagtt
      481 gatttatgat gcgggcgtgg attttgtctg ggttgactgg tccaataatc tggatttcga
      541 catgcccatg gacagggtat tgaaagacag aacatccagg gcggattttg ccatgattga
      601 aaggtctacc gtctatatgt ttgatgtcta cagtcaaatg gagaaagcac ctaaaataag
      661 tatctttatt ggctgtccgg caccgggtca aaaagaagga gtcactgacg gaagacttac
      721 aagaaaggcg gaccagatat acgactggtt tgtaaacaac ccggagcatc cggagtacaa
      781 ggatcagctc ttctactata acggcaagcc attgcttgtt gtatatgtag gtactccatc
      841 accatggcag agtggtgttc cggagtggga tgatgacagg tttaccgtaa gatacatgac
      901 aggctatgta acagagcaga aggggttgag ggatcctgac actctggaaa gcaagtatgg
      961 ttactggagc tgggaagaca gagtagctca aacctttaca gtgtccgatg gttatcctga
     1021 agccatgata gtttgtgcca gcacaagacc gcaggcagag gaaggagatc cgaattacat
     1081 tcctgctgtg gaaagacgta acggacaaac cttcaaggag cagtgggcaa gagccagact
     1141 gattggtgtc aggtttgcca tggtgggtac atggaatgag tacgttaccg gagaacagta
     1201 ttcggatgaa gtcagcaagg atattgagcc caatactgtc tggggtgaca tgtatttaaa
     1261 acttctaaaa gaagaaattg caaagttcaa agggaaaaac aaatagaaaa tatttcagga
     1321 gcagtaccag gacaggtatt gctccttgtt ttattgacaa atgccatgtg ttgatgtaat
     1381 atattattgt ccgctggggg agcccaagaa tggctgagat taaggtccgg acctttaaac
     1441 cccacgaacc tgtgtaggta atcctaccgt agggaagcgg tttcacatga acaatatatg
     1501 tgtaactaag cttccttcaa gaataattac ttggaggttt ttttatgaac aaaaacttgt
     1561 ttgagtttct cacggatggc accgagattc taaaatttga caagatattt gagaatccgg
     1621 tagtctttgg tattgtggtg ctagtagcca tcgctcttgt cgtttttctt gttctccaat
     1681 acaggaaaga aaaagatgcg ccaaagccca aacgtaaaac ggatgtaaaa gtgcttgtaa
     1741 caggagcttt gtgcatatca attgcgtttg tattgtcctt tatcaagctt ctggacctgc
     1801 ctcaaggcgg agctcttact cttgcatcag gtctgcctat tatcctgttt gcatatctgt
     1861 atggtccaaa gcatgggctt gctgctgctt ttgcatattc ccttttacag cttctgcaag
     1921 gtgcatatac agtaggaatc atccagtttt tacttgatta tccgcttgca tttacagcct
     1981 tcggtcttgc tggcttctac agaaagaata taattcctgg tataattgcc gggtatatgg
     2041 taagacttat gtgccacttt gtctctggag ttgttttctt tggagaatat gcaaaggaac
     2101 tgggatatgc agtggttcct tattcgttta tgtataatgc ggttttcttg attcctgaaa
     2161 tgattctgtg tataattatc gtattaatac ctcaggtaaa gagaatgatt ggaaacataa
     2221 ggaaagaact ggctgtatag aggtattgaa atacagtata aaggaagaag cgcttgaaac
     2281 ggaaaaaagt gattatagcg ttgctgattg cagtttgtct gcttggtttg ctggcattga
     2341 tagcaataaa tctgtacatg gtgaaatatt caaggcaata tatcctggat gatgaaagca
     2401 tcaaggacat ggatgccgaa tacatagttg ttctgggtgc aaaggtatac aaagacggaa
     2461 gactgagctt gatcctggat gatagggtga aggcatctgt aaagcttttt gaagaaggca
     2521 gagttccagc aattattatg agcggggatg cacaaaggga agactacaac gaggttgatc
     2581 ccatgaagaa aagagccata gagctgggcg ttcccgagga atgtattata acggatacga
     2641 tggggctttc cacctttgag tccatgaaaa ggcttaaaga tgagtttggt gttgacaagg
     2701 tgataatttg cacccaggag taccacatgt acagagcaat atacaatgca aggaaaatgg
     2761 gtattgaggc ttacgggtat ccggcagaaa aaatagatta cgcaaaccag ctgcagagag
     2821 atatccggga agcgattgca cgggttaaag actttatact ggtattgttt acaacttaat
     2881 tgttttgttt gaaaggggtt ggatttccaa tcccttttta catatttctg caacagttat
     2941 ataggttttg cttgaaacct ttttctttac agttagagag atgtaaacag gtatataatt
     3001 agcatgtgta atgggtagta ggcgtagaat gcatattgta taatcttgtt cattgtccgg
     3061 ttggttggat tatgaccttt ttttccgttg tacaggagaa ttggaacaag tgcaaaaaga
     3121 gcatacagtt gcacaaaagc ccaggaattg ttctggctta acagaatgta agaaaatatt
     3181 acaactgtat ggccggttat ggtggcgagg ttttttcttc tacaatgctt catgtacctg
     3241 aaacaaagaa ctgtcagtat gccgtagaat ccgtaatcaa cattgataag ttctgcggtg
     3301 aggcagaata atgaaaccag tattgtggat aatagtatca gtctaggttt gtcttcgtat
     3361 attttcttgg actcgtcaag ccagtaaaga agaagcaggg atagtgacag ggtaaagaag
     3421 atgttttttg acgaacttat gtcaatcagt ttgccttttg aaaacaggtt gaacggtatt
     3481 tctgatacaa gagcaaacag aaaaagcctc tttagatatc ttttgaagtc tttagtgtgg
     3541 tgatatcctt cggttagcag aaatgcgtat atgggaaaag caatcctgcc aattattctg
     3601 tatgagtatg ttattttcaa tgtgtatcct atatgatcta aaagcatcgc aatgcatgca
     3661 attattttta aagtaagtgc cgacatgtaa acctcttgtg tgtaaattat atttataatt
     3721 atattatcac aatatttggc attattctat tgacaatcct taagccttct gatattcttt
     3781 ctgcgcatgt ataatataaa ggaggggtat gaatgtacga gtgtctctgt ggttatgttt
     3841 acgacccagc tgagggagat ccagaaaacg atattccagc tggtacagct tttgaagatt
     3901 tgccagatga ctgggtttgt ccaatttgtg gtcttgataa aagtgctttc agcgaagtat
     3961 gataatgaaa gactaaaaat gccggatgtg tgttttttga catatccgga tttcaatatt
     4021 ttgggaggta ttttatgaac atttgtgtaa gttcctacag tttttccaga ctggtaaggg
     4081 aaggatcctt aaagccggag aatgttattt acaaggcgaa ggaaatgggc ttttacggta
     4141 ttgaatttgc aggtctttgg gttccggaag gaatgactgc tcttgactat gccaagcatg
     4201 ttaaagacct tgcagaggat gctggtatca agataacagg atacagtgtt ggtggaaatc
     4261 tgctttgcaa caaccaggaa gaggaaattg agagactgaa gggcgaagtg gatgttgctc
     4321 atgctcttgg tgtaaacata atgaggcatg atgtggcata tgagcccatg cctggaggtt
     4381 acaaaagctt tgagaacaat ctgccccttt tgataaaagg tgtaagagca gtaaccgagt
     4441 atgcagaaag ccttggggta aggacatcta ttgagaatca cggtttttac actcaggaca
     4501 gccacagggt ggaaatgctc attgatggtg tgaattccga caactatgga gctttattgg
     4561 acctgggcaa ctttctctgt gttgatgagg atcctgcaaa agctaccggt gttctcaaaa
     4621 actatgtatt cagtgttcat gcaaaagatt tctatttcca tgatggcgat gagactccca
     4681 gctcaccggg ttggtttatg acaagaggag g
//
LOCUS       DAONVY010000127         4709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13107_length_4709_cov_3.827245, whole genome shotgun sequence.
ACCESSION   DAONVY010000127 DAONVY010000000
VERSION     DAONVY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13107_length_4709_cov_3.827245"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="mreC"
                     /locus_tag="PLI11_04575"
     CDS             <1..698
                     /gene="mreC"
                     /locus_tag="PLI11_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPZ52171.1"
                     /translation="LATGIKDRFEAIGSYTDLINENKRLKEEVDKLQGYKNRNEQLET
                     ENQELRALLELKDYYQEYDLIAANIIAKDVTDWYNEFTLDAGTQEGVYIGCPVITSKG
                     LVGIVSKVSAHTCKVMTLVDEQNVIMARISRSNELVRLRGMTPENYEYLLKLDRISAS
                     SDLYVGDLLVTAESGGVYPKGIAIGTVIEISTIAETETRYAIVEPLVNLPKISEVYIL
                     SLPEKARITEDKE"
     gene            708..1334
                     /gene="mreD"
                     /locus_tag="PLI11_04580"
     CDS             708..1334
                     /gene="mreD"
                     /locus_tag="PLI11_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPZ52172.1"
                     /translation="MRKQRLESRLNKRLNKVFSSLIIDSSESVSKITKGRIIYISLSV
                     IFLLIIQEAVFNDLRIFNAKPNMAIVFLYTISVVLHVRPAMFAGLFAGLYVDIMYGRY
                     LGVYSLLFMLFAAFVSVISFEQVKLKTIWSLSIAIPSFLIYTMVESFTAKLIALARTG
                     GTVLYENYGVHMLKRILPVTLYDVIIFFILFVPIRLIWDKLGPKYEIF"
     gene            1347..3521
                     /locus_tag="PLI11_04585"
     CDS             1347..3521
                     /locus_tag="PLI11_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPZ52173.1"
                     /translation="MNKLFLNSLKERLAKIFKSRIFYVGLGITVAMTAISARLYYLQI
                     VQGEQHKDNAATINKVVLYTETPRGNIYDRNGILLATNRVAYKVQMVNVDRDQSFRDE
                     MYLKVINLLDEMGEDYNNPLKRYIDYPIAWGSYLEGEENEDRRKAWINGIALRKSDRD
                     KINTAKDAFDYLRNVVFKFDESYTDEEAYKIMILRYQTYTMGLSYMVPLTIADDVSSK
                     TMEIIEARHLEFPGIYTEEVYYREYVHDETLSHIIGYVRAINSEEYEALKDQGYDRNS
                     LIGKNGIERAAESLLRGVKGTRTVYQDIETGIVRELESTEPVPGDDIYLTIDVNLQRA
                     AYDALVKTIEDIIARKDNKKNFGDAKAGSVVVTDVRTGEVLAMANYPTYDNKIFLAPS
                     SDEEAQRAITELFQDPDSPSLNRATQGLYPVGSTFKTLVAIAALEEGVMTPYQKIKCL
                     GMIEVNNRVHRCNAYHYDIDMVRAIAESCNVYFYETGIKTGIKNLDKWAKAFGLGEKT
                     GIEIPEYAGYRNNEETMKLMESDIYHVWTPSDTAQASIGQLYNLFTPIQLSNYAAAIA
                     NGGYLNKPRLIAASVSNSGHVEKYEAERKKIEVKEETLKVVQEGMITMSKLSGIAKRE
                     YRGFPDGFAAGKTGTPQTGLEAFGRSSHSVYICYAPSDNPEIAISVVIEYGASGSNSI
                     AVAGEVLRAYYGDTGLGEGLPARKDGLGFGDGFRNFKSLIQG"
     gene            3647..4459
                     /gene="minD"
                     /locus_tag="PLI11_04590"
     CDS             3647..4459
                     /gene="minD"
                     /locus_tag="PLI11_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPZ52174.1"
                     /translation="MSEVIVVTSGKGGVGKTTTTANIGAALALEGKRVVLIDTDVGLR
                     NLDLVMGLENRVVYTLVDVIEEKCRGIKQALIKDKRFHKLYIVPAAPSGYRHQIKPEQ
                     IVKVCEQLKKEFDYIIIDCPAGIENGFEVAIAPADFAIVVTTPEVSAVRDADCVIGLL
                     DSNHVRDHKLVINKVRPEMVRKGEMLSINDIVEILAEELLGVIPDDEDVIVSTNRGEP
                     IVLAGRSSAGKSYRDIARRIMGQNVPLVDYTRKKGFMQKVFGKNERGYYYEA"
     gene            4449..4676
                     /locus_tag="PLI11_04595"
     CDS             4449..4676
                     /locus_tag="PLI11_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HPZ52175.1"
                     /translation="MRRNKGNYIGSKEIAKNRLKSCINSDRVKVDHDVMQMLEAEIRN
                     VVSSYIDTKGASFELIVEIGDGKNNGKDNST"
BASE COUNT         1563 a          815 c         1145 g         1186 t
ORIGIN      
        1 accttgccac cggaatcaag gacagatttg aggcaatcgg ctcctatact gaccttataa
       61 acgaaaacaa gagacttaag gaagaagttg acaaactaca aggttacaaa aatagaaacg
      121 agcagctgga gacggaaaac caggaattga gagcactgct cgaactaaag gactactatc
      181 aggaatacga cttgattgca gcaaatataa tagccaagga cgttacggat tggtacaatg
      241 agttcactct tgatgcaggc acgcaggaag gggtatacat cggttgccct gtgataacat
      301 ccaagggact tgtaggtatt gtatccaaag tatcggccca tacatgcaag gtcatgactt
      361 tggtggatga acagaatgtc atcatggccc gtatatcaag aagcaatgag ctggtaaggc
      421 ttagaggcat gactcctgaa aactatgaat atttgcttaa actggacagg atatcagcgt
      481 catcagacct ttatgtcgga gatctccttg tgactgctga gagtggagga gtatatccaa
      541 aagggatagc cattggcaca gttattgaga taagtacaat tgcggagacg gaaactagat
      601 atgcaattgt agaaccgctt gtgaatctgc caaagataag cgaggtatac atattgagcc
      661 ttccggaaaa agcaagaatt acagaagata aagaataggt gatcaagttg cgcaagcaga
      721 gacttgaaag cagactgaac aaaagattga acaaggtctt ttcgtcttta ataatagaca
      781 gttctgaaag tgtgagcaaa attacgaagg gccgaataat atacatctct ttatctgtaa
      841 tctttctcct tattatccaa gaagcagttt ttaatgatct gaggattttt aatgcaaaac
      901 ccaacatggc aattgtcttt ctctatacca tatcagttgt tttgcatgtc agacccgcca
      961 tgtttgcagg attgtttgca ggtctgtatg tggatataat gtatggcagg tatttagggg
     1021 tttattctct tttattcatg ttgtttgcgg cttttgtctc cgttattagt tttgaacaag
     1081 tgaaattgaa aacaatctgg agtctttcaa ttgcgatacc ctcattttta atatatacta
     1141 tggtggaaag ctttacagca aaactcatag ctcttgcaag aaccggcggt actgtattgt
     1201 atgaaaacta cggcgttcac atgttgaaaa ggatactgcc ggtaactctg tatgacgtta
     1261 taatcttttt catactgttt gtaccaataa gactgatatg ggacaaactg ggtccaaagt
     1321 atgaaatttt ctaggatggt gaaagcatga acaagctttt tcttaattca ttgaaagaaa
     1381 gacttgcgaa gatatttaaa tcaagaatat tctatgtcgg cctaggtata actgttgcca
     1441 tgaccgcaat ttcagcacgt ctgtattacc ttcaaattgt gcagggagag cagcacaagg
     1501 acaacgccgc cactatcaac aaggtcgttt tgtacaccga aactccaaga ggtaacatat
     1561 atgacagaaa tggcattttg ttggccacaa acagggttgc atacaaggtg cagatggtga
     1621 atgtggacag agatcagagt tttagggatg aaatgtattt aaaagtgatt aacttgttgg
     1681 atgaaatggg tgaagattac aacaacccgt taaagagata tatagattat ccaatagctt
     1741 ggggttcgta tcttgaaggt gaagaaaacg aagacagacg aaaggcttgg ataaatggta
     1801 ttgctttgag aaaatccgat agggacaaga taaacacggc caaggatgct tttgattatc
     1861 ttagaaacgt ggtgttcaaa tttgacgaat cctacacgga tgaggaagcg tacaagataa
     1921 tgattcttag gtatcagacg tatacaatgg gtcttagcta catggtgcct ttgacaattg
     1981 cggatgatgt gtcctcaaag acaatggaga ttatcgaagc aagacatcta gagtttccgg
     2041 gaatatatac ggaagaagta tattacagag aatacgttca tgacgaaacc ttgagccata
     2101 taatcggcta tgtaagagct ataaacagtg aggaatatga agccctcaaa gaccaagggt
     2161 atgatagaaa cagtctaata ggaaagaacg gcatagaaag ggcggcggaa tctcttcttc
     2221 gcggagtaaa gggaaccaga acagtatatc aggatattga aacgggaatc gtaagggaac
     2281 ttgaatcaac agaacccgta ccaggggatg atatatacct taccattgat gttaatcttc
     2341 aaagggctgc ttacgatgcg cttgtaaaaa caatagaaga cataattgca aggaaggaca
     2401 acaaaaagaa ctttggtgat gcaaaagccg gctctgttgt tgttacggat gttaggacgg
     2461 gagaagtgtt ggctatggca aactatccga cttatgacaa caagatattc cttgctcctt
     2521 ccagtgatga agaagctcaa agagcaataa ccgagctttt tcaggatcca gatagtccaa
     2581 gtcttaacag ggcaacccaa ggtttgtatc ctgtaggttc aaccttcaaa accctggttg
     2641 caatagcagc tctggaagaa ggtgtaatga ctccctatca gaaaattaag tgccttggaa
     2701 tgattgaagt aaacaacagg gtgcacagat gcaatgcgta tcattatgat attgacatgg
     2761 taagggctat cgctgaatcc tgtaacgtgt acttctatga gaccggaata aaaacaggaa
     2821 taaaaaattt ggacaagtgg gctaaagcct ttggccttgg tgaaaaaaca ggtattgaga
     2881 taccagaata cgcaggatac agaaacaacg aggaaaccat gaagcttatg gagtctgata
     2941 tttaccacgt atggacgcca tcagatacag ctcaggcatc aatcggacag ctgtataacc
     3001 tgtttacacc gattcagctc agtaactacg ctgctgccat tgcaaatgga ggatacctga
     3061 acaagccacg tttgattgca gcatctgtat ccaacagcgg tcatgtggaa aaatatgaag
     3121 cggaaagaaa gaaaattgaa gtcaaggaag agaccttgaa agtagttcag gaaggcatga
     3181 ttaccatgtc caagctgagc ggtattgcga aaagggagta cagaggtttt ccggatggtt
     3241 ttgcagcagg caaaacggga actcctcaga ctggtcttga ggcattcgga agaagctccc
     3301 actcggtgta tatctgctat gctccatccg ataatcctga gattgcgatt tccgttgtta
     3361 ttgagtatgg tgcatcgggt tcaaactcca ttgccgttgc aggcgaggtt ttgagagcat
     3421 actacggcga caccggtctt ggtgaagggt tgcctgcaag aaaagacgga ctgggatttg
     3481 gagacggttt tagaaatttc aaatctctca tacaaggata aagttgagga gagtagtcga
     3541 accaatagac atacaactgg acacgcccaa tacgcatcaa taattgccat acaaacaata
     3601 ttcgggcttt cctcttaata atacacatta ttatggaggt aggaatatga gtgaggttat
     3661 tgttgttact tcgggcaaag gtggtgtcgg aaaaaccact acaacggcaa atatcggtgc
     3721 cgcactggca cttgaaggaa aacgtgtggt attaatcgat acggacgtgg gacttagaaa
     3781 tctcgatttg gttatgggac ttgaaaacag agtggtatac acacttgtag atgttatcga
     3841 ggaaaaatgc aggggaatta aacaggcact gataaaggac aagcgttttc acaagctgta
     3901 tatcgtccct gcggcgccat cgggatacag gcaccagatt aagccggaac aaatcgtaaa
     3961 agtatgtgaa cagctaaaaa aagaatttga ttatataata attgactgcc ctgcgggaat
     4021 agaaaacggg tttgaagtgg caattgcacc tgcggatttt gcaattgtgg tcacaactcc
     4081 ggaggtttct gcagtaagag atgcggactg tgtcataggt ttgcttgatt caaatcatgt
     4141 aagagaccac aagctggtca ttaacaaggt aagacctgaa atggttcgaa aaggggaaat
     4201 gctcagcatc aatgacattg tggagatact ggcggaagaa ctccttggag taatcccgga
     4261 tgacgaggat gtaatagttt caaccaacag aggagaaccc atagtactgg caggaagaag
     4321 ctctgcgggc aaatcctaca gggacatagc aagaagaatc atgggacaaa acgtccccct
     4381 ggtcgattac accaggaaaa agggctttat gcagaaagtg tttggtaaaa acgaaagggg
     4441 gtactactat gaggcgtaac aagggtaatt acataggctc gaaggaaatt gcaaagaaca
     4501 ggttgaagtc ctgtataaac agtgatagag taaaggttga ccatgatgta atgcagatgc
     4561 ttgaagcgga aattaggaat gtggtttcaa gctatataga tacaaaggga gcatcttttg
     4621 aattaatagt tgaaataggt gatgggaaaa acaatgggaa agataatagt acttgatgaa
     4681 cttacagcaa acaaaatagc agcaggaga
//
LOCUS       DAONVY010000128         4706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13115_length_4706_cov_3.907332, whole genome shotgun sequence.
ACCESSION   DAONVY010000128 DAONVY010000000
VERSION     DAONVY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13115_length_4706_cov_3.907332"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLI11_04600"
     CDS             complement(<1..185)
                     /locus_tag="PLI11_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPZ52176.1"
                     /translation="MIHVSIWLEDEIIVGFEIKGHAGYADKGYDIICSAVSAISYTAV
                     GYFENKYNPEGKMDKIS"
     gene            complement(188..499)
                     /gene="rplU"
                     /locus_tag="PLI11_04605"
     CDS             complement(188..499)
                     /gene="rplU"
                     /locus_tag="PLI11_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPZ52177.1"
                     /translation="MYAIIVTGGKQYKVSEGSTIFVEKLNAEPEQEVVFDEVLAVSKG
                     DEVVFGTPKVEGVSVTARVLKNGKGKKIRIIKHKPKKGYMKRQGHRQPYTQVVIEKIN
                     A"
     gene            complement(613..2016)
                     /locus_tag="PLI11_04610"
     CDS             complement(613..2016)
                     /locus_tag="PLI11_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HPZ52178.1"
                     /translation="MRKITVIFIVLLLLLSGLISINAEGERRFLLAGADTVEAGSTYV
                     LTVKIDGTNIYGCQAILEYNPEQLELKGAVLIENEFTEEWSYQEMEGLPEGELGFVVL
                     DDNKLANPINQETGIINITFTVKEELEVGTKVKICLKKQVITYIDDKVSGVSPEDVEF
                     ETTVIKKVLSNNDYLKSLNVTNGTLVPSFNKNTVNYTVSVPYSITKLQFTALCEDEKS
                     TLQVNNPDLVEGGKTNVTLTVTSEAGTKRVYTIQVTRAQKPEEPKSSNNKLESLEADY
                     PLSPLFHSDITEYTMEVPNEVTEIILSITTQDTKCTYVVEGGKDLVVGENTVTVVCTA
                     EDGSVRNYVITVTRQEGQQATPSPTDEEPTESPEETSKPTQPAETPGVIVVSDPKGMP
                     YWLVIVLIIIFTGVGFMFGVTINIKGAKEYNDVDDDDNYIESNIVESDFGRKETPKKK
                     ERNTLFIIEDSFRDDDK"
     gene            complement(2037..2951)
                     /locus_tag="PLI11_04615"
     CDS             complement(2037..2951)
                     /locus_tag="PLI11_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPZ52179.1"
                     /translation="MRTKAGNVFVVVLIVSILLFPLCETNAGEESLSVSVSLNKSLAS
                     YGDLVDVTVSLLGYSSNNIGVIEACIPIDTSLFSVVEDSIQNHLQTEEGTPIITMGHR
                     KGSSEVYYSYVDVVKLVEKGGEVFSFQLEVIGEDVDVLKEIYLSSVILADKDFNNLEF
                     DMNTVSIYITDYNISVEDRLLSKIHKDTTVKDFTESLSALNVEIIDKNDKRVDEEEYV
                     GTGMKARFELNGEINEYLICVTGDANGDGEINVTDLIAVRAHLLNKKKLEGIYFTAAD
                     VNNNGEINITDYIAINADVLNKKYIVPN"
     gene            complement(2932..3588)
                     /locus_tag="PLI11_04620"
     CDS             complement(2932..3588)
                     /locus_tag="PLI11_04620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPZ52180.1"
                     /translation="MKKFLAFCMAVVILVTLATGCSKEKNDNPVLVLNIKSERFGIEG
                     IIEIEMYPDKAPNTVKNIIYLANLGFYDGIQICQVRPDKLVEIGDPKNVQLGGVRYAI
                     KGEFKENGFTQNDIKFERGVVAMSRYAASYDSAVGDFFIQLDDTEEYDGKFAAFGRVT
                     KGLELLDEISRVKNVGRALGYEPVYPIFIESVYLKLKGKQYGEPKRLTREFFGANKGW
                     "
     gene            complement(3603..4193)
                     /locus_tag="PLI11_04625"
     CDS             complement(3603..4193)
                     /locus_tag="PLI11_04625"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPZ52181.1"
                     /translation="MDKIKFKPSTLLSPVPVVMVSLGDKSVSNIITVAWVGTVNSEPP
                     MISISVRRQRYSYDIINKHKTFGVNLVSTDIVERADWCGVKSGKEIDKFKECNFTKFY
                     GDITGVPLIKESPVNIECEVKEVVSLGSHDMFIGEVVAVYTDASLSDDKGKLDLAKAN
                     LISYAHGEYFALDKRLGFFGYSVAREEVLRRRMGKE"
     gene            complement(4256..>4706)
                     /locus_tag="PLI11_04630"
     CDS             complement(4256..>4706)
                     /locus_tag="PLI11_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPZ52182.1"
                     /translation="DELIVSITDNGVGMDSEMLSKVVDPFTTGRTTRNVGLGIPFFKQ
                     ACEMTGGHVEIVSRRKVGTTIKGKMVVSHIDRLPLGDIGETFQTLIMSKPEIDYVLRL
                     ENLMGSAAISTMEIKKELEDVPITTYEVLIWLKTFVNTKVSEILGDI"
BASE COUNT         1316 a         1146 c          695 g         1549 t
ORIGIN      
        1 tacgatattt tgtccatctt cccttccggg ttgtatttgt tttcaaaata tccaactgcc
       61 gtataactta ttgcagaaac cgcagaacaa ataatatcat atcctttgtc tgcgtatccc
      121 gcatgccctt ttatttcaaa tccaacaatt atttcatctt ccagccaaat actcacatgt
      181 atcatgatta agcgtttatt ttctcaataa ctacctgggt ataaggttgt ctatgtccct
      241 gtctcttcat ataacccttc ttaggtttgt gcttaattat gcgaatcttc tttcctttac
      301 cattcttaag cactctggct gtaacagaaa caccttcaac tttcggagta ccaaaaacaa
      361 cttcgtcgcc tttcgacaca gccaaaactt cgtcaaaaac aacttcctgc tctggctctg
      421 cattaagctt ttctacaaat atggtacttc cttcggaaac tttgtactgt tttcctcctg
      481 ttacgattat tgcgtacatt attgcacctc cacattaaac tcgcctgcca agggtactga
      541 aggatcagat ttaaaaaccc ttacagagcg gcaccaaaag attttaacac agcaatgtca
      601 cattgtcaaa tattatttgt catcatcacg gaaagaatct tcaataatga acaatgtatt
      661 cctttccttc ttttttggtg tttccttcct tccgaagtca gattcaacta tgttactttc
      721 tatgtaatta tcatcatcat caacatcgtt atactctttc gcacccttta tattaatagt
      781 aacaccaaac atgaatccaa cgcctgtaaa gataataatc aaaacaataa ccagccagta
      841 aggcatgccc ttaggatctg aaacaactat gacgcctggt gtttcagcag gttgcgtagg
      901 tttgcttgtc tcctcaggtg actcggtggg ctcctcatct gttggagatg gtgttgcctg
      961 ctgtccttcc tgtctagtaa cagtaataac atagtttctt acactgccat cttccgctgt
     1021 gcacacaacc gttactgtgt tttcacccac cacaagatcc tttccacctt ctaccacata
     1081 tgtgcatttt gtatcctgtg tggttatgct cagtattatt tccgtaacct cattaggtac
     1141 ttccattgta tactcggtta tgtcagaatg aaacagtgga gaaagcggat aatcagcttc
     1201 aagactctcc aatttattat tgctgctctt tggctcctcc ggtttctgtg ctcttgttac
     1261 ctgtatcgta tacactctct tggtacctgc ctcggacgtg actgttaatg tcacattcgt
     1321 tttcccacct tcaacaaggt caggattgtt cacctgaaga gtactttttt catcttcgca
     1381 caaagctgta aactgaagct tagttatgga atatggaaca cttacagtgt aattgactgt
     1441 gttcttgtta aagcttggca ccagcgtacc gtttgtaaca ttaagacttt taagataatc
     1501 attgtttgac agcactttct taataacagt agtctcaaac tccacatcct cagggctgac
     1561 accacttact ttgtcatcaa tataggttat gacctgtttc ttgaggcata ttttaacctt
     1621 tgtgccgacc tccagttcct ctttcactgt aaaagtgata ttaataatgc cggtttcctg
     1681 gttgattgga tttgcaagtt tgttgtcatc caaaacaaca aaccccagtt ctccttccgg
     1741 gagaccttcc atctcctgat aagaccattc ctcggtaaac tcattttcaa tcaatacggc
     1801 acccttgagt tcaagctgtt caggattgta ctccagtatg gcctggcaac catatatatt
     1861 ggtaccatct attttgacag ttaacacata tgtactgccg gcttcgacag tatcagcacc
     1921 tgccaataga aaccttcttt ccccttctgc atttattgaa atcaaaccgg aaagaagaag
     1981 taacaataca ataaaaatca cagttatttt cctcatattt cctccatcaa atgtgtttaa
     2041 tttggtacaa tatacttttt atttaataca tctgcattta tagctatata atctgttatg
     2101 tttatttccc cattattatt tacatcagca gcggtaaaat atatgccctc cagctttttc
     2161 ttgttaagta agtgggccct gacagctatc aaatcagtta catttatctc tccgtcaccg
     2221 ttggcgtcac cggtaacaca tattaaatat tcatttatct ctccattcag ttcaaaccgt
     2281 gctttcatgc cggttcccac atattcttct tcatccaccc tcttgtcatt cttgtcaatt
     2341 atctcaacat ttaatgcact taaactttct gtaaaatcct taactgtggt atccttatgt
     2401 atcttggaaa gcaatctgtc ttcaacactt atgttgtaat ccgttatgta aatggaaaca
     2461 gtattcatat caaactcaag attattaaaa tccttgtctg caagaataac agaagacaaa
     2521 taaatttcct tcaaaacatc cacatcttct ccaattacct caagctgaaa agaaaacact
     2581 tctcctccct tttcaaccag tttaactaca tccacatagc tatagtaaac ctcactgctt
     2641 ccttttctat gacccattgt aataattggg gttccttctt ctgtttgcaa atgattttgt
     2701 attgaatcct caactacaga aaaaagactt gtatctattg ggatacaagc ttcaataaca
     2761 cctatattgt tagagctgta tcccaataga gacacagtca cgtcaaccaa atcgccgtaa
     2821 ctggctaaac ttttgttcag ggatacactt acggacaaac tttcttcccc ggcattcgtt
     2881 tcacatagtg gaaacagcag tattgaaact attaaaacaa caacaaatac attaccagcc
     2941 tttgttcgca ccgaaaaact cccttgtcag tctctttggt tcgccgtact gttttccttt
     3001 aagcttaaga taaacacttt ctataaagat gggatatacc ggctcatatc ccaaagctct
     3061 tccaacattc ttgactctgc taatctcatc cagtagctcg agtcccttgg tcacacgtcc
     3121 gaatgcggca aacttgccat cgtattcttc ggtgtcgtca agctgaatga aaaagtctcc
     3181 aacagcagaa tcataactgg cagcatatct tgacatggcg actactcctc gttcaaactt
     3241 aatatcgttc tgggtgaatc cattttcctt gaactctccc tttatcgcat atctcacgcc
     3301 accaagctgg acattcttgg gatccccaat ctccaccagc ttgtcgggtc tgacctgaca
     3361 tatttggatt ccatcgtaga atccaagatt tgcaagatat attatgtttt tcaccgtatt
     3421 tggtgccttg tcgggataca tctcaatttc aataataccc tcaataccga acctttcgct
     3481 cttaatgtta agcaccaata ctggattgtc atttttttct tttgaacatc cggtagccaa
     3541 tgtaaccaga attacaactg ccatacaaaa ggccagaaac tttttcataa acacatcccc
     3601 aattattctt tacccattct tcttcttaga acttcctctc ttgccactga gtaaccaaaa
     3661 aaacccaatc ttttgtccaa ggcaaaatac tcaccatgag catatgatat caaattagcc
     3721 ttggctaaat caagcttccc tttatcatcc gacaaggacg catctgtata aactgcaacg
     3781 acctccccaa taaacatatc atgggaaccc agggatacaa cttccttaac ctcacactca
     3841 atgtttacag ggctttcttt aatcaatgga accccagtaa tgtcaccgta gaacttagtg
     3901 aagttgcatt ccttgaattt gtcaatttcc ttaccggact tgacaccgca ccaatctgct
     3961 ctttctacaa tatcagtaga caccaaattc acaccaaaag tcttgtgttt gttaataata
     4021 tcgtaggaat atctctgtct tctgacagaa atcgatatca ttggtggctc tgaatttaca
     4081 gtacccaccc aggctacagt tatgatattc gaaacacttt tgtctcccag cgataccata
     4141 actaccggca caggacttag caatgttgat ggtttgaatt tgattttatc catttaaatc
     4201 actccttttg gtacagatat attatataac atttaccgaa atagtaaaac atctttcata
     4261 tatctccaag tatctctgaa accttggtat taacaaaggt tttcaaccat atcagaacct
     4321 catacgttgt tattggcaca tcctccagtt cctttttgat ttccatggtt gatattgcag
     4381 cacttcccat taaattctca agccttagca cataatcaat ttctggcttt gacataatca
     4441 atgtctgaaa agtttcccct atatctccaa gtgggagtct gtcaatatgc gaaacaacca
     4501 ttttcccttt aatggttgtt cccactttcc ttcttgatac aatttcgacg tgcccacccg
     4561 tcatttcaca tgcctgtttg aaaaaaggaa tcccgaggcc aacatttctt gtagttctgc
     4621 cggttgtaaa aggatccaca accttcgaaa gcatttcgct gtccatgcct acaccattat
     4681 ccgttatgct tactataagt tcgtcc
//
LOCUS       DAONVY010000129         4695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13155_length_4695_cov_3.308836, whole genome shotgun sequence.
ACCESSION   DAONVY010000129 DAONVY010000000
VERSION     DAONVY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13155_length_4695_cov_3.308836"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLI11_04635"
     CDS             complement(<1..776)
                     /locus_tag="PLI11_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52183.1"
                     /translation="MTVNEIGASTYVKNEVGMLIGNENETKSHDSFFNVFMLALEGIA
                     GEIADTKDEEELAKYMVPMFVPVNHENQIPLITEEQLGKPWANMEEVNVNEVEYDVFP
                     MEVKVQDVDIEESTNDETFEKLVFIEGDEPEVFGIKTQDLDVSQEPKEVFPVDNTVRR
                     KNVTELHTVEKKPDIPNEGVEDKQPVSTKPVSEERFVLKDDNKLHNLHIEKPVQKPKE
                     IRMQRDKENPQIERFDIGIPQNTLEVDNENLSVSTNHVVE"
     gene            complement(812..1396)
                     /locus_tag="PLI11_04640"
     CDS             complement(812..1396)
                     /locus_tag="PLI11_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52184.1"
                     /translation="MQDKNVNLKKKKNNEIPPVLAGVSVFAICVFLVILFCMLAYFNV
                     GGLGLDVINTLRKNEAIALDEQKAMEQEKKNIEELKLEVEKMQEDLAKREEELEKGWE
                     EIAQEEAQLIKEKKKYEDLVNDLMPEFNNIQEVAEIYRAMDAAKVAELLEKQDNMALK
                     VRIIKALDVSFASQVLSKMSPDKAAAILERIHDY"
     gene            complement(1411..1836)
                     /gene="fliJ"
                     /locus_tag="PLI11_04645"
     CDS             complement(1411..1836)
                     /gene="fliJ"
                     /locus_tag="PLI11_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPZ52185.1"
                     /translation="MKKFEFTLNSLLRIKNSLERQAKQKMAEAVSRRDKCQEEIEEIE
                     RRKELARTNNKSFTAHEYLMFSRYYSDLERMRKSKTCELEAIEKEIEEIRQELVTIMQ
                     ERKVLEKLREKQYEEYLVELGKEQDKLVDDMMTYKITIA"
     gene            complement(1839..3161)
                     /gene="fliI"
                     /locus_tag="PLI11_04650"
     CDS             complement(1839..3161)
                     /gene="fliI"
                     /locus_tag="PLI11_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HPZ52186.1"
                     /translation="MDLSIVERKIKNSDLYTYTGSVSEVTGLMIESEGPIAKMGEVCL
                     IYPTDGVSEPIRTEVVGFSGNKLVLMPLGHVNGIAQGCTVVATNHTFRVPVAEELVGR
                     ILDGLGQPIDNKGPVTPTEYYGIDNDPPDPLMRERITKVLPLGIKAIDGLLTCGQGQR
                     LGIFAGSGVGKSTLLGMIARNAVADLNVITLVGERGREVRDFIEKDLKEEGLRKSVLI
                     IATSDQPALVRVKAAMLGTSIAEYFRDKGKNVLFLMDSLTRFAMAQREVGMASGEPPV
                     SRGYTPSVYAMLPRLLERTGNSSKGSITGLYTVLVDGDDMNEPISDTVRGILDGHIVL
                     SRKLANRNHYPAIDVLASVSRVMPDIVTREHYDIANTIKSKMAVYKDAEDLINIGAYK
                     KGANQDIDEAILLNKPIEGFLRQRIEEAHGFDKTVDMMREILRIGAGS"
     gene            complement(3170..3865)
                     /locus_tag="PLI11_04655"
     CDS             complement(3170..3865)
                     /locus_tag="PLI11_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPZ52187.1"
                     /translation="MIRIYKAQEVKYEKAVSGQIGREVKTTLSDRMVSIEEKERQAEE
                     ILLKAQLRAKEILDTAEENANILLAQTAEKSQEMYDAVHKKAKEEGYAAGRKEAEKLI
                     EESDKKAKEIIKEAYEIREKIISSMSSEILDLSFSIAEKVLNYELERNTKAFMSLINS
                     AASKTEDDNAKILVSAEDYMNHQQVLSEDDRVEPDDSLKKGEIKVISKKGIVDASLDK
                     QLEKAKIAAGERN"
     gene            complement(3858..>4695)
                     /gene="fliG"
                     /locus_tag="PLI11_04660"
     CDS             complement(3858..>4695)
                     /gene="fliG"
                     /locus_tag="PLI11_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPZ52188.1"
                     /translation="YSEFYDMYVAQSYISQGGIAYAKEVLEKAIGSQKAYEIIEKLSS
                     TLQVRPFGFARKADPSQLFSLIQNEAPQTIAIIFSYLHPQQAGLLLGMLPKDKQISVI
                     EKIATMDRTSPEYIKEIERIIERKMSASAMDDFTVTGGLQSVVDILNGVDRGTEKFIL
                     ETLSKINNDLADKIRKKMFVFEDIVKLDSRSVQKVISDTDVNTLAIALKGATQDVHKV
                     IFDNISSRLRQIIQEDMEYMGPVRVRDVEEAQQKIVNVIRKLEEAGEIVIVRGKEDVL
                     ID"
BASE COUNT         1069 a         1222 c          788 g         1616 t
ORIGIN      
        1 ctctcaacaa catgatttgt tgacacagac agattctcat tatccacctc aagggtgttt
       61 tgtggaattc caatatcaaa cctttcaatc tgtggattct ctttgtccct ttgcatcctg
      121 atttcctttg gcttttgaac tggcttttca atgtgcagat tgtgcagctt gttatcatct
      181 ttaagcacga aacgctcttc agaaacaggc tttgttgata ctggttgttt gtcttcaaca
      241 ccctcattag gtatatcagg ctttttttca actgtatgta actctgtcac attctttctt
      301 ctgactgtgt tgtcaacggg aaacacttct tttggctcct gactgacatc aaggtcttgc
      361 gtcttgatac cgaaaacttc cggctcatca ccttcaatga aaaccagttt ttcaaaggtt
      421 tcatcatttg tactttcttc aatgtcaaca tcctgcacct ttacttccat gggaaaaaca
      481 tcatattcta cttcattgac attgacttcc tccatgttag cccaaggttt tcccaattgt
      541 tcctctgtaa tcaaaggtat ttggttttca tgatttacag gaacaaacat gggcaccatg
      601 tactttgcca attcctcttc gtcttttgta tctgctattt ccccggcaat accttccaac
      661 gcaagcataa atacattgaa aaaggaatca tgagattttg tttcattttc attgccgatt
      721 agcattccaa cttcattctt tacatatgta cttgctccaa tttcatttac tgtcaccttc
      781 tcacctccct ttactgcaat caattacggg attaatagtc gtgtattctt tcaagtatgg
      841 ccgctgcctt gtcaggactc atcttgctga gcacctgaga tgcaaaactt acatccaaag
      901 ctttgattat cctgaccttc aaagccatat tgtcctgttt ttcaagcagc tccgccactt
      961 tggcagcatc catcgccctg tatatttcag caacttcctg tatgttattg aactcgggca
     1021 tgaggtcatt taccaaatcc tcatatttct ttttttcctt aatcaactga gcttcctctt
     1081 gagctatttc ttcccagccc ttttcaagct cctcttccct ttttgcaaga tcctcctgca
     1141 ttttttcaac ctcaagctta agctcctcaa tattcttttt ttcctgctcc atagcctttt
     1201 gctcatccag agcaatcgcc tcgttttttc tcaacgtgtt aattacatca aggcccaaac
     1261 cacccacatt gaaatatgcc agcatgcaaa acaaaattac tagaaacacg catatggcaa
     1321 acacagatac tcctgccaga acggggggaa tttcattatt ctttttcttc ttcagattca
     1381 catttttatc ctgcatcttc ataccctcac ttaagctatt gtgattttgt aagtcatcat
     1441 gtcatctaca agcttgtcct gctcttttcc aagttccacg agatactctt catactgctt
     1501 ttccctcagt ttttcaagta cctttctctc ctgcataatg gttaccagtt cctgtcttat
     1561 ctcttcgatc tccttctcaa ttgcttcaag ttcgcaagtc ttgcttttcc tcattctctc
     1621 caaatcggag tagtatcttg aaaacatcaa atattcgtgg gctgtaaagc ttttgttgtt
     1681 tgttctcgcc agctccttac gtctttcaat ttcctcaatc tcctcctggc atttatccct
     1741 tctcgaaaca gcctccgcca ttttctgttt tgcctgtctc tccagggagt ttttaatcct
     1801 aagcaagctg tttagagtaa attcaaactt cttcatacct aactacctgc cccaatcctg
     1861 agtatctctc tcatcatgtc taccgttttg tcaaaaccat gtgcttcctc aatccgctga
     1921 cgcaggaaac cttcaatggg cttgtttaaa agaatcgcct cgtcaatatc ctggtttgca
     1981 cctttcttgt atgcgccaat gtttatgagg tcttccgcat ccttgtacac agccattttg
     2041 ctctttatgg tatttgctat gtcatagtgt tctcttgtta ctatgtccgg cattaccctg
     2101 ctgacgctgg caagaacatc tatggcggga tagtggtttc tgttggcaag cttccttgaa
     2161 agaacaatat gtccgtcgag tatacctctt actgtatccg atatgggctc attcatgtcg
     2221 tctccatcaa ccagaacggt gtacaacccg gtgattgagc ctttgctgga attgcccgtt
     2281 ctttcaagaa gtcttggcag catggcgtat acggagggcg tatatcctct tgaaacagga
     2341 ggctctcctg acgccatccc cacttccctt tgcgccattg caaaccttgt caatgagtcc
     2401 atgaggaaca atacattctt tcccttgtct ctaaaatatt cagctatcga tgttccaagc
     2461 attgccgcct ttactctaac caatgcaggc tggtcgctgg tggcaataat tagcactgat
     2521 ttcctaagtc cttcttcctt caagtctttt tctataaaat cccttacttc ccttcctctc
     2581 tcgcccacca gggttatgac gtttagatcc gccactgcgt ttctggcaat cataccaagc
     2641 agtgtactct tgccaacacc gctgcctgca aagatgccaa gcctttgacc ttgaccgcaa
     2701 gtcagaagtc cgtcaatcgc ttttatgccc aaaggtagaa ccttggttat tctttctctc
     2761 ataagagggt cgggagggtc attgtctatt ccataatatt ctgttggtgt aactggtccc
     2821 ttgttgtcta ttggctgacc aagtccgtct aaaattcttc ccactaattc ttccgcaaca
     2881 ggaacacgga atgtgtgatt tgttgcaact acggtacaac cctgagctat accattcaca
     2941 tgaccaagag gcataagaac aagtttgttc ccagagaacc caaccacctc tgtcctgatg
     3001 ggctcactga caccatcggt tggatagatg aggcaaacct cgcccatctt ggcaattggt
     3061 ccttccgatt ctatcatcag tcccgttacc tcgctgacac tgccggtata tgtgtataag
     3121 tcactgtttt tgatttttct ctccacaatg gacaaatcaa ttaccttggt caatttcttt
     3181 ctcctgctgc aatttttgcc ttctcaagct gtttgtctaa actggcatcc acaattccct
     3241 ttttggagat tacctttatc tcacctttct taagactgtc atcaggttca actctgtcgt
     3301 cctcacttaa aacctgctgg tgattcatgt agtcttcagc acttacaagt atctttgcat
     3361 tatcgtcttc cgtttttgac gccgcactgt ttatgagtga cataaacgcc tttgtatttc
     3421 tctccagctc ataattcaaa accttctctg cgatactgaa ggataaatcc agaatctcgc
     3481 tactcatgga gcttattatc ttctccctaa tctcatacgc ttcctttata atctccttgg
     3541 ccttcttgtc gctttcctca atcagctttt ccgcttcctt ccgtcctgct gcatagcctt
     3601 cctcttttgc ctttttgtga actgcatcat acatctcctg ggacttctcg gcagtctgcg
     3661 caaggaggat atttgcattt tcctcagccg tatcgaggat ttcctttgca cgaagttgtg
     3721 cctttaaaag tatttcctct gcctgccgtt ccttctcctc aatggacacc attctgtcgc
     3781 ttagcgttgt ttttacctca cgcccgattt gtccggatac cgccttctca tactttactt
     3841 cctgcgcctt gtatattcta atcaattagg acatcctcct ttcctcttac tattacgatt
     3901 tctcctgcct cttcaagctt tctgattaca ttaacaatct tttgctgggc ttcttcaaca
     3961 tcccttaccc tgacagggcc catgtattcc atgtcttcct gaataatctg tctgagacgg
     4021 gaggagatgt tgtcgaagat aaccttgtga acatcctggg ttgcaccttt caatgcaatg
     4081 gcaagagtat tcacatccgt atcgctgata accttctgta cggagcgtga atccagcttg
     4141 acaatgtctt caaatacaaa catcttcttc ctgattttat ctgctagatc gttgttaatc
     4201 ttggacaagg tttcaagtat gaatttctcg gtacctctgt ctacaccatt caatatgtct
     4261 accaccgact gcagtccgcc ggttactgta aagtcatcca tggcagaagc agacatcttt
     4321 ctttcaataa ttctttcaat ttcctttatg tactcgggag atgtcctgtc cattgtcgct
     4381 attttctcta ttactgatat ctgcttgtct tttggcagca tgccgagtaa tagaccagcc
     4441 tgctgcggat gcagatatga aaagataatg gcaattgtct gaggcgcttc attttgtatg
     4501 aggctgaaca attgtgaagg atcagccttt ctggcaaatc caaagggtct tacctgcaga
     4561 gtggaactga gtttttctat gatttcataa gccttctggc tgcctattgc cttttcaagc
     4621 acttcctttg cataggcaat acctccttgg gatatgtaac tttgggcaac atacatgtca
     4681 tagaactcgg agtaa
//
LOCUS       DAONVY010000130         4689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13174_length_4689_cov_3.763919, whole genome shotgun sequence.
ACCESSION   DAONVY010000130 DAONVY010000000
VERSION     DAONVY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13174_length_4689_cov_3.763919"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLI11_04665"
     CDS             complement(<1..1050)
                     /locus_tag="PLI11_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52189.1"
                     /translation="MTFFYTKYIWAVWKRAKNVNILTVVYMIPFLTFIMSLLSTNIFP
                     VIAQYAFINNSNYNYMYKLDYMTDQKILEDLNQESLFIGNHNSIYVYKEDIITAGMQS
                     IIHLGTPDSSLTHTKFTIDNFLSFNGELKDSSIYLSYDCTKALNLHLGDTCTIEFNKY
                     DHTTKTSYKRKVEFKVAGILKPNYEDSQPSRSPNNNLSLAILDEESLNWLKDKSCHFD
                     DRYYYIYYANEFVDLSKYQEDNINFSYSTKEDLLRKTKEYYLSPSVLVSKGATIIVSF
                     ATLFAILLFELRYLRKKHSRNIRIICMLGLSRKGVKELYMGLALANFLIASIISFVLL
                     KFIILDLIIKLYYTFL"
     gene            complement(1047..2273)
                     /locus_tag="PLI11_04670"
     CDS             complement(1047..2273)
                     /locus_tag="PLI11_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52190.1"
                     /translation="MTRMILLGRVFFSRKSALLSMLNNLLKLIIFFFIILFSIIPIVR
                     YQYTIDKLLYSNYDALVQGCSEHMYSRFDKDANVTGLVAITTTMSSTLVHGSNRTTTR
                     LAAVYDDNLDALTEYVLTDELFVKKDDSLMNKSNALLLSYWTSKNIKADIGDVVRIED
                     LDLEYVLAGIYSDSYSNASAIVLGSSHEGKAAKFFDNLLKGRLYGTVYLKFEDIKAGL
                     DDIDDNYYRDFPLYRKYGENYLVEAADDELLQAKSSAQIRTEQLRRTELSLKYSDSPF
                     DKSYAYYIIPIGMMMVCLLTVQENNKRIQLGLKPVAILSISGISKMHIFLYFLFDSFI
                     KTSILQLLTIYITLLIFRAQEIYTPSDLMLGYTLLYIFSILIASVISAHISIKKLGEE
                     HIMAALNDEDIQEDLL"
     gene            complement(2298..2969)
                     /locus_tag="PLI11_04675"
     CDS             complement(2298..2969)
                     /locus_tag="PLI11_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52191.1"
                     /translation="MSFIEFKEVDLTFQTKVQRIPIFKGLNLEIEKGEFITIAGPSGK
                     GKSTLLNLVSGFVKPTGGEIWVDGKNLSKMNDTQVCDYRNKRIGYIFQTFNLIQQFNV
                     RDNVAVPLLLSGMKKKQAYEKVEELLDKVGMTNRILEYPRTLSGGEQQRVAFARAIAN
                     NPDIILADEPTGNLDTKNGELIVTLLMEANKENGTTVLCVSHDERIIEKGQRTILIEE
                     ITCVE"
     gene            complement(2984..4147)
                     /locus_tag="PLI11_04680"
     CDS             complement(2984..4147)
                     /locus_tag="PLI11_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52192.1"
                     /translation="MLIIRYVLALWKRKTLGQLISLLYITPTFIIIICTVLQMLMPVA
                     SSIIFETNSNYTYMYSGDFFTVTDNGIDISNDAVYFLNSDDAATYLYKPFVISSKYDN
                     TFIVDGHPFSEINKLDNLYIAMKEKEFSFGLFTRKNLITRYNTSNNVLSTYPEIYISY
                     NSARILEVAPGDKIIVKVNKGYIECQVSGILRPNYEDLSYSRNWLDYTEVTSFPALLI
                     VDENTFYDIANGHHDISVRVFTNKEFVPNSFDMYLKASKINSYKELLMSSSLITRLAS
                     QVLMIAAVLIIVIQLEYGFTQKKNAKDIEVLAKLGMNKKQISLTLTTHVCIVCFICLI
                     LASLICRFIYFDILIDMYCETLVYLITYLLLAMICILILVIRYIFAKKYYSSF"
     gene            complement(4159..>4689)
                     /locus_tag="PLI11_04685"
     CDS             complement(4159..>4689)
                     /locus_tag="PLI11_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52193.1"
                     /translation="YLEHGDNWRDKIKENEFLYKIYLESAGKGFEKHSTLLKYAEKYS
                     TIDTRVVVFYALAASIAISLLFISKNTKAINTGMKSFGVLSASGMPSKTFFIYFVLTT
                     FIEQTVLLVLVHLGKTLFFIYRNDTWFYYMTNSFVRIRIYFPIIIGIVLSSLFTGIYA
                     KSIISKRKILSALSSE"
BASE COUNT         1446 a          842 c          780 g         1621 t
ORIGIN      
        1 caaaaaggtg taatatagct ttataatcag gtcaagtatt atgaatttaa gcaaaacaaa
       61 tgatattatg ctggcaatca gaaagtttgc gagtgcaaga cccatgtaca actcttttac
      121 tccttttctg gacaggccaa gcatgcatat tattcgaatg tttcttgagt gcttttttct
      181 caagtatctc agttcaaaca acagtattgc aaacagggta gcgaatgaaa caattattgt
      241 tgcccctttt gatacaagta cactaggaga cagatagtat tctttggttt ttcttaacag
      301 atcctccttt gtactgtatg aaaaattaat attgtcctct tgatacttgg acaaatcaac
      361 aaattcattt gcatagtaaa tgtaatagta cctgtcgtcg aaatgacatg atttatcctt
      421 taaccaattt agtgattctt catccagaat tgcaaggctt aagttattgt ttggtgatct
      481 ggagggttgg gaatcctcat agtttggttt caatattcca gcgactttaa actcgacttt
      541 tcttttatat gatgtttttg tagtatgatc atatttatta aattcaatgg tacaggtatc
      601 tcccaaatgc aaatttaatg ctttggtaca atcataggac aaatagatac tgctatcctt
      661 taattctcca ttaaaggata agaagttatc tattgtaaac ttggtatggg tcaagctgct
      721 gtcgggtgta ccaaggtgta ttatgctctg cataccggcg gttataatat cttccttata
      781 cacatatatc gagttatggt ttcctatgaa caaagattcc tgattcagat cctcaagtat
      841 cttttggtct gtcatatagt caagtttgta catataattg taattgctgt tgttaataaa
      901 agcatattgg gcgataacgg gaaatatgtt ggtcgacaat agtgacataa taaaagtcag
      961 aaacggtatc atgtatacca ccgtcagtat gtttacattt ttcgcccttt tccaaaccgc
     1021 ccatatatat ttcgtataaa agaaagtcat agcaaatcct cctgtatgtc ttcatcgttt
     1081 aatgccgcca ttatatgttc ctctccgagc ttctttatgc tgatatgggc agatattact
     1141 gacgcaatta atatgctaaa aatatacaga agtgtatagc caagcatcaa atcagaaggt
     1201 gtgtatatct cttgtgctct gaatattaac aaggttatgt aaatggtcaa cagctgtaga
     1261 atactcgttt taataaatga gtcaaacagg aagtataaaa atatatgcat tttggatata
     1321 ccgctaatgc tcagtatggc aacgggcttc aaccccagct gtattcgctt gttgttttcc
     1381 tggactgtca gcaggcatac catcatcatt cctatgggga tgatatagta tgcatagctc
     1441 ttgtcgaagg ggctatctga atactttaaa gaaagctctg ttctcctaag ttgttctgtt
     1501 ctaatttgtg cgctgctctt tgcctgtaga agttcatcgt cagcagcttc taccagatag
     1561 ttttcgccgt attttctgta aagaggaaaa tccctataat aattatcgtc tatgtcatcc
     1621 agcccggctt ttatgtcttc gaactttaag tagacagtgc cgtataatct tcctttcaga
     1681 agattgtcaa aaaacttggc tgccttaccc tcgtgtgatg aacccaaaac aatagcggaa
     1741 gcatttgaat aggaatccga ataaatgccg gccaaaacat attcaaggtc taggtcctct
     1801 attctaacaa catctcctat gtcagccttt atgtttttgc ttgtccaata actaaggagt
     1861 aacgcattgc ttttattcat tagactgtca tcttttttta caaacagctc atcagtaagc
     1921 acatactctg ttaatgcatc aagattatcg tcatagacag cagccaatct agttgttgtc
     1981 ctgttgcttc catgaacaag tgtactactc atagtagtgg ttattgcaac aagtccagtt
     2041 acattagcat ctttatcaaa tctgctgtac atatgttcgg agcatccttg taccaatgca
     2101 tcatagttgc tgtatagcag cttatctatt gtatattgat atcttacaat cggtattata
     2161 gagaataaga ttatgaagaa aaaaattatc agtttcaaca gattgttgag catggaaagc
     2221 aaggctgatt tccttgagaa gaaaactctg cccaataata tcattcttgt cataaaattg
     2281 tctccttgta tttctaccta ctctacgcag gttatttctt caattagtat cgttctttgt
     2341 cctttttcaa taattctctc atcatgggac acacaaagta cagttgtacc attttcctta
     2401 tttgcttcca tgagaagagt aacaataagc tcaccgttct ttgtgtccag gtttccagtg
     2461 ggttcgtcag caaggattat gtcaggattg tttgcaatgg ctcttgcaaa ggcaaccctc
     2521 tgctgctcgc cacccgacaa ggttcttgga tattcaagaa ttctgtttgt catgccgacc
     2581 ttgtctaaaa gttcttccac tttttcataa gcctgcttct tcttcatacc tgatagaagc
     2641 agaggaactg ccacattgtc ccttacatta aactgctgaa tgagattaaa ggtttggaat
     2701 atgtaaccta tacgtttgtt tctgtaatca catacctgtg tgtcattcat tttgcttagg
     2761 tttttgccgt caacccagat ttctccgcct gtaggtttga cgaaacctga aacaaggttt
     2821 aaaagtgttg attttccttt gccggatggt ccagcaatgg tgatgaattc tcctttttca
     2881 atctccagat tcaatccctt gaatattggt attctctgta cttttgtctg aaatgttaga
     2941 tcaacttctt tgaactcaat gaaactcata tgcaacctcc tcttcaaaat gatgaataat
     3001 atttctttgc aaaaatatat cggattacca aaattaatat acatatcatt gcaagtagta
     3061 gatatgttat taagtatact aaagtttcac aatacatgtc aattaaaata tcgaaatata
     3121 taaagcgaca tatcaatgat gccagaatta aacagataaa gcaaacaata catacatgtg
     3181 tggtcaatgt taatgatatt tgctttttgt tcattccgag ctttgctaat acctcaatat
     3241 cttttgcatt ctttttttgt gtaaatccat actctagttg tattacaatt attaatacag
     3301 cagcaatcat aagcacttgt gaagccaatc gtgttataag actgctcgac attaaaagct
     3361 ctttataaga gttaattttg cttgctttta aatacatatc aaaactgtta ggtacaaatt
     3421 ctttatttgt aaaaacacgt actgaaatgt catgatggcc attagctatg tcataaaatg
     3481 tattctcgtc aacaataagg agtgctggaa atgacgttac ctcggtatag tcgagccagt
     3541 tgcgtgagta agacaggtct tcgtaatttg gtcttagaat tccactgact tggcattcaa
     3601 tgtaaccttt attaacttta actataatct tatctcccgg agcgacttca agaattctgg
     3661 ctgaattata ggaaatatat atttctggat aggttgaaag aacgttattc gacgtattat
     3721 acctagttat caaatttttg cgtgtgaata atccaaatga gaactccttt tccttcatgg
     3781 caatatatag attatccagt ttgttaattt cggaaaatgg atgcccatcg actataaatg
     3841 tattgtcata cttggaagat attacaaatg gtttatataa atatgtggca gcatcgtctg
     3901 aattcaaaaa atagacggca tcattactta tatcaatacc gttatcagta acagtaaaaa
     3961 aatccccact atacatgtat gtataattgc tattggtttc aaaaataatg ctggatgcaa
     4021 caggcatcaa catctgtagt actgtgcata taattatgat gaatgttggg gttatatata
     4081 ataatgatat taactgacca agtgtttttc ttttccatag agctaagaca tatcttataa
     4141 ttaacataat catactcctt attccgatga taatgctgac aatattttac gtttgcttat
     4201 aatgcttttt gcataaatac cagtgaataa agatgatagt actattccaa ttattatggg
     4261 aaaataaata cgtatccgta caaatgaatt agtcatgtaa taaaaccatg tgtcatttct
     4321 atagataaaa aataatgttt ttccaagatg gactaatact aacaagacag tctgttctat
     4381 aaaagtagtt aatacaaagt agataaaaaa tgttttgctt ggcattccgg aagctgacag
     4441 tacaccaaaa cttttcatgc cagtatttat agccttagtg tttttgctga taaaaagcag
     4501 actgattgca attgatgcag ccaacgcata aaaaacaact actcttgtat caatggtact
     4561 gtatttttct gcatatttta agagggtact atgtttctca aatcccttac ctgctgattc
     4621 taaatatatt ttatataaaa attcattttc cttaatttta tctctccaat tatctccatg
     4681 ttctaaata
//
LOCUS       DAONVY010000131         4683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13204_length_4683_cov_3.815039, whole genome shotgun sequence.
ACCESSION   DAONVY010000131 DAONVY010000000
VERSION     DAONVY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13204_length_4683_cov_3.815039"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLI11_04690"
     CDS             complement(<1..723)
                     /locus_tag="PLI11_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ52194.1"
                     /translation="MVVDNKKVKTVWLLFFVLLAIAYLAGCVANNGKEDVEYYYTFTD
                     SLDNTVNLKDKPQRVVSLVGSYAETWMLAGGELVGVTNDVITERGMDVPEGTRIVGTI
                     KEPNVEEILDVSPDFVLLSPDIESHVSISVILEKVDIPFAFFKVEHFEDYLSMLDICT
                     DITGDKELYKKNGLDVKKQIDDILAKIDKTKNPDMLFIRAFSSGAKAKNDDNNACRIL
                     NDLGTVNIASKHNSLLEDLSMEV"
     gene            complement(1058..2146)
                     /locus_tag="PLI11_04695"
     CDS             complement(1058..2146)
                     /locus_tag="PLI11_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPZ52195.1"
                     /translation="MGNNSLRIIYGDNPKRMIMELLEVVQPEKDIERDALVGIKPNLV
                     LAKPSSSGATTTPAIVEGLIEYLQLKGHMNIVIMEGSWVGDRTREAFKVCGYEKISKE
                     YNVPLIDLQRDTYSEFEYGGVRINICNYAVNVDYLINVPVLKGHCQTNLTCALKNMKG
                     CIPNTEKRRFHTMGLHKPIAYLSKMLKQNLVIVDGMNGDLNFEEGGNPVQMNRIIAGK
                     DPVLVDAYAAELLGFDIGEIPYIGMAEDIGVGTTNLANMDIVELNKDTGKRKFRPTRR
                     VQHLVRNVVEDSACSACYGSLVYALERLDDKNLLNKLEEKIYIGQGFKDKTLDGIGIG
                     ICTSRFAKCVKGCPPKAKDIVDFLAKSY"
     gene            2275..2466
                     /locus_tag="PLI11_04700"
     CDS             2275..2466
                     /locus_tag="PLI11_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPZ52196.1"
                     /translation="MACSNRIKCTCTYTSCSRHGKCCECVAYHRRSGEVPGCFFSASG
                     ERTYDRSIENLYRDYKKNG"
     gene            complement(2652..3422)
                     /locus_tag="PLI11_04705"
     CDS             complement(2652..3422)
                     /locus_tag="PLI11_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52197.1"
                     /translation="MISVQSTPNLTGVTISGDYDDLYSLVEAFHEITINEYSEKHHKY
                     IGISIRVLGLCYDIRHAYQGDRMVELVDNKMSVYNMKRHSIIVPSTNVYYSCNYLYPE
                     MFFVMLALNELVNLRIKELAKTGSLHKGILDRRVIWDDTIATIRSFQAKFVNCVKETV
                     SPGTFSRWLSLMNSEGTHIEGISDQYVDLLNIKYLRMSKDNRLKNFNYFARRIADYRN
                     DDEHYEIKRMLTRAAREYNCHPSDIRMAGTNYPEQIEW"
     gene            3653..3844
                     /locus_tag="PLI11_04710"
     CDS             3653..3844
                     /locus_tag="PLI11_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52198.1"
                     /translation="MSIPIPKSPSAKTDSTIESLIGKYGQRITDFLSRDGAPLSAELK
                     KILTVRKFINIYGPLGTGV"
     gene            complement(4014..>4683)
                     /locus_tag="PLI11_04715"
     CDS             complement(4014..>4683)
                     /locus_tag="PLI11_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52199.1"
                     /translation="PVGAVAFMMCHGHESWHPSPEFYYKRGGGPMFDMGPYYLAALVN
                     LMGSVREVMGMTTKAVEERIITSQPLYGKAIEVEVDTHVAALLRFENGAVATIITSFD
                     VWNSTLPRIEIYGTKGTLIAPDPNTFGGPVLLATRDGGIFREIPLTHIYAENSRGIGL
                     SDMAQCIIEEIDDNKASGRMANHVLEIMCAIDESQKSGKSYIMKSRVDRPQALGSNLV
                     KGFV"
BASE COUNT         1360 a         1040 c          804 g         1479 t
ORIGIN      
        1 tacttccata cttaagtctt caaggagaga attgtgtttc gaagcaatgt ttacagtacc
       61 aaggtcgttt agaattctgc atgcgttgtt gtcatcattc tttgcctttg caccactgga
      121 gaacgctctg ataaacaaca tgtcaggatt ctttgtcttg tctattttgg caagtatgtc
      181 atctatctgt ttctttacat caagaccatt tttcttatac aactccttgt cacctgttat
      241 gtcggtgcat atgtccagca tggaaagata gtcttcaaaa tgttccactt tgaagaatgc
      301 aaaaggtatg tctacctttt caagaattac tgatatactt acatgactct ctatatccgg
      361 agaaagcagt acaaaatcag gagaaacatc cagtatttct tccacattgg gctcttttat
      421 cgtgccaaca atcctggtcc cctcaggcac atccataccc ctctcggtta tgacatcatt
      481 agttacacca acaagctcgc cacctgcaag catccaggtt tcagcatagg aaccaacaag
      541 agaaacaacc ctttgaggct tgtctttcag attgacagta ttatctaatg agtccgtgaa
      601 ggtatagtaa tattccacgt cttcttttcc attatttgcc acgcaccctg caagatatgc
      661 aattgcaaga agtacaaaga ataaaagcca cactgttttt acttttttat tatcaacaac
      721 catcctatca tccttaatat attttactct acaataatca tataacttaa caaatgtatt
      781 gtaaaatatg attgtttgat agttgtaata ggttttacct atatgtttgt gatagtatgg
      841 ttgtctgtaa agaaatatgc gaggaggttt aaatggaatt ctcataacca tgatgctgaa
      901 gaaaagatcg attgagaaat aacggtttgg aggaaatgag caccggaatt tgtccccggt
      961 gctatcagaa ctacaaacct attttaagac ttgtagcaga cctcgttgta gagtctgaaa
     1021 aactatggct gtccatattg aattagaaca gccatcctca ataacttttt gccaggaaat
     1081 caacaatatc ctttgctttt ggaggacatc cctttacaca tttggcaaaa cgcgacgtgc
     1141 atatacctat acctattcca tcaagcgttt tgtccttaaa cccttgtcca atatatattt
     1201 tctcctcaag tttattcaaa agattcttgt catcaagcct ctccagtgca taaacaagac
     1261 ttccgtaaca cgccgagcag gcactgtctt caacaacatt tcgtaccaaa tgctgcactc
     1321 ttcttgttgg cctaaacttt cttttgccag tatccttgtt cagctcaaca atatccatat
     1381 ttgcaagatt tgttgtacct acaccaatat cctcagccat accaatatac ggaatttcac
     1441 caatatcaaa acctaacaac tcagcggcat atgcatcaac aagcacaggg tcttttcctg
     1501 caatgattct attcatttga acagggtttc caccctcttc aaagtttaga tctccattca
     1561 ttccgtcaac tattaccagg ttttgcttca acattttgct tagataagca attggcttgt
     1621 gcaaacccat ggtatgaaat cttcttttct ctgtgttcgg tatgcaccct ttcatattct
     1681 tcaacgcaca tgtaagattg gtttgacagt gtcccttaag cacgggaaca tttatgagat
     1741 aatcaacatt tacggcataa ttgcatatgt taatcctcac accaccatac tcaaattccg
     1801 aataagtatc cctttgcaga tcaataagcg ggacattata ttccttcgat atcttttcat
     1861 atccacaaac cttgaacgct tcacgggttc tgtcgcctac ccaggaacct tccatgatca
     1921 cgatgttcat atgtcctttt aattgcaggt attcaattaa gccttccact attgccggtg
     1981 tggttgttgc gcctgaactt gacggctttg ccaacacaag attcggcttg attccaacaa
     2041 gagcatccct ttctatatcc ttttcaggct gtacaacttc aagaagttcc ataatcatcc
     2101 gctttggatt atcgccataa atgatgcgca gtgaattatt acccatcatg catatcatcc
     2161 tccccatata gcttacctca acacacctct ttcgtaaagc aggggtaatt tgttgaaatc
     2221 cgctgtgaat taatttataa tgttgttgtt aataaattct acggaggttt aaaaatggct
     2281 tgcagcaata gaatcaaatg tacttgtaca tatacgtcat gttcacgtca tggcaaatgc
     2341 tgtgaatgtg ttgcttatca cagaagaagc ggcgaagttc caggatgttt cttttctgca
     2401 tccggtgaaa gaacttatga caggtccata gagaacctgt acagagacta caagaagaac
     2461 ggataatcta ttcagcgaga tatactttgc aatatatgtt ttttgatgcc ttaataaaca
     2521 aaaaggcatc ttatttttct tgccgttgca acacatctcc taaattacaa caattggaag
     2581 agaaaaaata tgcagaatat acaagccaag ggttgtatgg ttgtaaggta cctgaatatt
     2641 gtattaggct tttaccattc aatttgctca ggataattcg tacccgccat ccttatatca
     2701 ctcggatgac agttgtactc tctggctgct ctcgttaaca tcctctttat ttcataatgt
     2761 tcgtcatcat tcctgtaatc cgctattcgt cttgcgaagt agttgaaatt cttaagccta
     2821 ttatccttgg acatcctaag atacttgata ttcaacaaat ctacgtactg gtcagatatg
     2881 ccttctatat gtgttccttc actattcatt aaagacagcc atctgctgaa ggtcccaggt
     2941 gatacggttt ctttgacaca gtttacaaac ttggcctgga aactgcggat ggttgcaata
     3001 gtatcatccc agataaccct cctatccagt attcctttat gcaaagaacc tgtctttgcc
     3061 agttccttaa tacgcaaatt cacaagttca ttcaaagcca gcataacaaa aaacatctca
     3121 ggataaaggt aattgcagct gtagtacaca ttggttgatg gcacaattat agaatgcctt
     3181 ttcatgttat aaacgctcat tttgttatct acaagctcca ccatgcggtc tccctgatat
     3241 gcatgcctta tgtcatagca aagcccaaga accctaatag agataccaat gtatttatgg
     3301 tgtttttctg aatactcatt tattgttatt tcatgaaacg cttctaccaa gctgtataaa
     3361 tcatcgtaat ctccgcttat ggtaacgcct gtcaggtttg gtgtgctttg tactgaaatc
     3421 attgttacat tcaatccttt aatctggttt cttttcttgc atcatttgta agtaccgagc
     3481 agaagtggaa cctttatcag catgtttatt gctcatttca tgccatcaag aaccattgta
     3541 ctagaattcc gtataaaatc aagactttac aaagcctgaa accttttcct tttgtcaaag
     3601 gtaaagcgga atgtaatcag atttgtagga ggtttgaaga aatctggatg aattgagcat
     3661 tccgatacca aaatctcctt cagccaaaac tgacagcacc attgaaagcc taattggcaa
     3721 atatggacag agaattacgg atttcttgtc aagagatggt gctcctcttt ctgctgaact
     3781 aaagaaaatc ctgacagtca gaaaattcat aaacatatac ggtccattgg gtactggagt
     3841 ttaaagcata ccgaaggaat attacacatc agaggatcca gcaggcacaa gtcactaccg
     3901 aatatgacag agtgagcctg cttgtataag gcagcatata ctaacaccgg tattgaaaga
     3961 caatacagtt aatctgaaaa cgccttctat ttaaagaagg cgttttttca taatcaaaca
     4021 aaacctttta caagattgct cccaagggct tgaggtctgt ctaccctgct cttcattatg
     4081 tatgactttc cacttttttg cgactcatct attgcacaca ttatttcaag cacatggttt
     4141 gccattcttc cactggcttt gttgtcgtct atttcttcaa ttatgcactg ggccatgtcg
     4201 gacagcccaa tacctctact gttttcagca taaatatgtg ttagaggtat ttctctgaaa
     4261 atgcctccat ccctggtcgc aagcaaaaca ggaccaccaa aggtattcgg atccggtgca
     4321 atcaaggttc ctttcgttcc atagatttct attctaggca gagtactgtt ccatacatca
     4381 aagctggtaa taatggtggc aactgcacca ttttcaaatc tcaatagagc agcaacatga
     4441 gtatcaacct caacctcgat agctttgcca tacagtggct ggctggttat aattctttct
     4501 tccacagcct tggtagtcat acccatgact tcccttacgc tacccataag gtttacaagt
     4561 gcggccaagt agtatggacc catgtcaaac atgggacctc cgcctctttt gtaatagaat
     4621 tctggtgatg gatgccagct ttcgtgtccg tggcacatca tgaaagccac agctccaaca
     4681 ggt
//
LOCUS       DAONVY010000132         4681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13212_length_4681_cov_3.088629, whole genome shotgun sequence.
ACCESSION   DAONVY010000132 DAONVY010000000
VERSION     DAONVY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13212_length_4681_cov_3.088629"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLI11_04720"
     CDS             <1..437
                     /locus_tag="PLI11_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HPZ52200.1"
                     /translation="IAQRAKCKEVYLFDSHADLGYGGRASLEFEISCANWLGKLFIDG
                     LIHKAYIIYSPNTYEDKDFFRQQNEKFDIEYLNIEDLDDGVEMSNIHIARSGTWTPPW
                     YDKDLKSFLEYTGLKYNVVDMKERFFDADNLTLADMINYLFA"
     gene            541..1221
                     /locus_tag="PLI11_04725"
     CDS             541..1221
                     /locus_tag="PLI11_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52201.1"
                     /translation="MSKKNIKVIITCLILVLIVILSSCDGTRKGSYIEAKDRLTGNDE
                     LRINIKLQKLYGGSKHLFLDDISAKELADYINKYDDENFNLSASVYQDKYVLITKADK
                     NDEFLNYYMLFVRGMKTFFCPVAVLKNDNTDVEIYMPFHLLIDASNRLESELSVNGIM
                     YKTVGTKKQFFDFYNTYERYDVEVLGDKFVVEDKVDGVKFEISFQYEKRPAQQNEFDL
                     FVTFDISS"
     gene            complement(1245..3080)
                     /locus_tag="PLI11_04730"
     CDS             complement(1245..3080)
                     /locus_tag="PLI11_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ52202.1"
                     /translation="MKRMIDLVKNKVEMLEGGSFKYTGIVDETIHLVQEKQLKDANYW
                     KLFVEQYRKGNVDDHDRGWRGEYWGKMMRGASLTYLYTQDEELYNLLEASVYDLLTTQ
                     DELNRFSTYSIECEFSEWDMWSRKYILLGSMYFLDICKDEELKNKLVNALVRHADYII
                     SKVGDEEQGKIPVTKTSIFWNGLNSSSILEPFVRLYVLTGFKRYLDFVRHIIKCGGTD
                     GFNVFEAADENKLYPYEYPVTKAYEMMSCFEGLLWFYRVTREEKYLSAAKNFANKVIE
                     SDITVIGSAGCTHELFDNSIINQFDPAYDGIMQETCVTVTWLKFLYQLLQITGDKSYA
                     DHIEVSIYNALLGSINTEGNERINTTPVFDSYSPLRKASRGRATGGLKDIAPDTYYGC
                     CIAIGAAGTALSALSSVGRTEKGFQFNLYYNGIIKTKTPKGNNIEFGINTTYPVGEDI
                     DININLDREEELTLDFRLPFGGKLYVNDNEIDTDTVTRVWKAGDKACIKTNLKIRLLK
                     DTDILPEAAERKERYGVLLRGPIVLARDARISNDLDTPLAFQTDNEGYVTSKFLETKA
                     PFRTLLAYELELEGGKYTFVDYAHAGRIWDENLPMAAWIIINDKI"
     gene            complement(3064..4392)
                     /locus_tag="PLI11_04735"
     CDS             complement(3064..4392)
                     /locus_tag="PLI11_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52203.1"
                     /translation="MTVSVNFDKITGRIKPLHGIGNAPLLGTNPKLFPYLKEAGIPYS
                     RLHDTGGRYGGGCFVDINNVFRDFDRDPKDPDSYDFAFTDWLITELVQQGVKPFYRLG
                     TTIENSHRIKAYYIYPPKDKLKWAQICEGIIRHYNEGWASGFYYDIEYWEIWNEPDNE
                     PNIEDNPMWKGTMEEYFELYKVASNYLKDKFPHLKIGGYASCGFYYLSEAVVESANSS
                     TRTGYFIHFFNEFLKFITSEENKSPLDFFSWHSYANPQENIKYAKYARKMLDEYGLYD
                     TETILNEWNPGIQYRSTSKDCANIAAMFCALQKAPVDKAMYYDGQVHTSYGGMFDPVK
                     QDIFKAYYAFYAFNKLYELSNEIECSSSKEGIYTIAAIDTNTARILISNPTEKEYNLA
                     IQTYTRKKITSVRKHIVDRDKNFEETDENRLTPESIILIEYEFERNEKND"
     gene            4560..>4681
                     /locus_tag="PLI11_04740"
     CDS             4560..>4681
                     /locus_tag="PLI11_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HPZ52204.1"
                     /translation="MVRGLYTAGTGMLTQRNKINVIANNVANVETTGYKEDLMLS"
BASE COUNT         1280 a          907 c          975 g         1519 t
ORIGIN      
        1 atatcgcaca aagagcaaaa tgtaaggaag tatatttgtt tgactcccat gctgatttgg
       61 gttatggggg tcgtgcctct ttggagtttg agataagctg tgccaactgg ctgggaaagc
      121 tctttataga cggtctgatc cataaagcat atattatcta cagccccaat acatatgaag
      181 acaaggactt tttcagacag cagaatgaaa aatttgacat tgaatattta aacattgagg
      241 accttgatga tggagttgag atgtccaata tacatattgc aagatccgga acatggactc
      301 ctccttggta tgacaaagac cttaagtcgt tccttgagta tactggtttg aagtacaatg
      361 tagtggacat gaaggagcgt ttctttgatg cggacaacct tacgctggct gatatgatta
      421 actatttgtt tgcataagat taacagtttg ttgttacaga tgacattgac aatatcttcc
      481 tgtaaaaata aaattagctt ataaattggt acgattattt tttaaaaagg atgatttgta
      541 atgtcaaaga agaatatcaa agtaatcatt acatgtttga ttctggtcct aatcgtaatc
      601 ttgtcttcat gcgatgggac aaggaaaggc agctacatag aggcaaagga tagacttacc
      661 ggaaatgatg agctgagaat aaacataaag cttcaaaaac tttatggagg cagcaagcat
      721 ctgttccttg atgacatttc agccaaagag cttgcagact atatcaacaa gtatgatgat
      781 gaaaacttta atctctccgc cagcgtttac caggacaagt atgttctcat tacgaaggct
      841 gacaagaatg acgaattttt aaactactat atgctgttcg ttcgtggaat gaaaacgttc
      901 ttttgtccgg ttgctgtact taaaaatgat aatacagatg ttgaaattta catgccattt
      961 catttattaa tagatgcaag caacagattg gaatccgaat tgtctgttaa cggaattatg
     1021 tacaagactg ttggtaccaa gaagcaattt tttgacttct acaacaccta tgaaagatat
     1081 gatgtggaag tattgggcga caaatttgtt gttgaagaca aggtggatgg tgtaaagttt
     1141 gagataagct ttcaatatga gaaaagacct gctcagcaaa atgaatttga tttgtttgtg
     1201 acatttgaca tttcaagtta agagggctgc gtgaagctac cctcttaaat tttatcgttg
     1261 ataataatcc aagctgccat agggaggttc tcgtcccata tcctgcctgc atgagcgtaa
     1321 tctacaaagg tatatttccc accctcaagc tctaattcat atgcaagaag agttctaaaa
     1381 ggagccttgg tttctaaaaa cttgcttgtg acataacctt cattgtctgt ttggaatgca
     1441 agaggtgtat ccaaatcatt acttattctg gcatcccttg caagtacaat gggtcctcta
     1501 agaaggactc cgtatctttc ttttctttct gcagcttcag gcagtatgtc ggtatcttta
     1561 agaagcctta tcttaagatt tgtttttata caagctttgt ctcctgcctt ccaaaccctt
     1621 gtgactgtat ctgtgtcaat ttcattatcg tttacataca gtttgccacc aaaaggaagc
     1681 ctgaaatcaa gagtgagttc ctcctctcta tcaagattta tattaatatc tatatcttct
     1741 cctaccggat aagttgtgtt gattccaaac tcaatgttat tgcccttcgg tgtcttcgtt
     1801 tttataattc cattgtagta taggttgaat tgaaaacctt tttcggttct tccaacactg
     1861 gaaagagcgg aaagggcagt accagcagct ccaattgcaa tgcagcagcc gtagtaggta
     1921 tccggagcaa tgtctttaag gccgcctgtg gctcttcctc tgcttgcctt tcttaaaggg
     1981 ctgtagctgt cgaatacggg agttgtgttg attctttcat tgccttccgt atttattgag
     2041 ccaagcaggg cattgtatat ggagacttca atatggtcgg cataggattt gtcacctgtt
     2101 atctgcaaaa gctggtatag gaatttaagc caggtaaccg ttacacaggt ttcctgcatg
     2161 attccatcgt atgctgggtc gaattggttg ataatggagt tgtcaaatag ttcatgagtg
     2221 catcctgccg aaccaattac agtaatgtca gactctatta ccttgtttgc aaagttttta
     2281 gcagctgaaa gatacttttc ttccctggtt accctgtaaa accaaagaag gccttcaaaa
     2341 caggacatca tctcataggc ttttgtgacc ggatattcat aggggtacag cttgttttca
     2401 tcagctgcct caaatacatt gaagccatcc gttccaccgc atttgataat atgtcttaca
     2461 aagtcaaggt atcttttaaa tccggtaagc acatacaacc ttacaaaagg ctccaggatg
     2521 gaactggaat taaggccgtt ccagaaaatg ctggttttgg ttaccggtat ctttccttgt
     2581 tcttcatctc caactttgga gataatgtaa tctgcgtgcc ttacaagagc attaacaagt
     2641 ttgttcttaa gttcttcgtc cttgcatatg tctaggaaat acatgctgcc tagtaaaata
     2701 tattttctac tccacatatc ccactcgcta aactcgcatt caatagagta ggtggagaac
     2761 ctgttcagct cgtcctgggt cgtaaggagg tcgtatacac ttgcttcaag caggttgtaa
     2821 agttcctcat cctgggtgta gagatacgta agggaggcgc ctctcatcat cttgccccag
     2881 tattcaccac gccagcctct gtcgtgatcg tccacgtttc cttttctgta ctgctcgaca
     2941 aacagtttcc agtagttggc gtccttcagc tgtttttcct gaaccaggtg tatggtttca
     3001 tccactatgc cggtatactt gaatgaaccg ccttccagca tttccacttt gtttttaacc
     3061 aaatcaatca ttcttttcat tcctttcaaa ctcatactca atcaaaatta ttgattcagg
     3121 agttaatctg ttttcatccg tttcctcaaa gtttttatcc ctgtccacaa tgtgctttct
     3181 tacagaagtg attttttttc ttgtatatgt ttgtattgca agattatact ctttttcagt
     3241 cggatttgat attagaattc ttgcagtatt tgtgtctata gctgcaatgg tgtagatgcc
     3301 ttccttgctg cttgagcatt caatttcatt cgaaagctca taaagcttgt tgaatgcata
     3361 gaaagcgtag taggctttga atatgtcctg tttgacaggg tcgaacatgc ccccgtaact
     3421 tgtgtggacc tgtccgtcgt agtacatggc tttgtcgaca ggggcttttt gaagggcaca
     3481 gaacatggct gcaatatttg cacagtcttt tgatgtgctt cgatactgaa tgcctggatt
     3541 ccattcgttc aggatagttt cagtgtcgta taagccgtat tcatccagca tcttccttgc
     3601 atatttcgca tacttaatat tctcttgagg attggcgtag gaatgccatg agaagaaatc
     3661 aaggggggat ttgttttctt cagaggttat gaatttcaaa aactcattga agaagtgtat
     3721 gaagtaaccg gttcttgtgg aggagtttgc actctccacc acggcttctg aaagatagta
     3781 gaaaccacag cttgcatacc ctccgatctt tagatgagga aatttgtctt ttaggtagtt
     3841 tgaggccact ttataaagtt caaaatattc ttccatggtg cctttccaca tggggttgtc
     3901 ctcaatattg ggttcgttgt caggctcgtt ccatatctcc cagtattcaa tatcgtagta
     3961 aaagccgctt gcccagcctt cgttatagtg gcggattatg ccttcacata tttgtgccca
     4021 tttcagtttg tcttttggag ggtagatgta ataggctttt attctgtggc tgttttctat
     4081 ggtggttccg agtctgtaaa acggctttac tccctgttgt acaagctcgg taatcagcca
     4141 gtcggtaaag gcaaagtcat aggagtcagg atcttttggg tctctgtcaa aatcccgaaa
     4201 gacattgttt atgtcaacaa aacagccacc gccgtatctt cctcctgtat cgtgaagacg
     4261 tgaatagggg atgccggcct ctttgaggta gggaaacagc ttcgggtttg ttcccagaag
     4321 tggtgcattc ccgattccgt gcagaggctt tatcctgcct gtgattttgt caaaatttac
     4381 acttactgtc attccatcag ctcctgttgt acgtatcttt atgttttggt aaatatatca
     4441 taggagaagg ctttattcaa ctgataaaga tttgtatttt gacaccaaaa atgctaaagt
     4501 ttattgtgcc tttgtcgatt atattactga acagagaata tctaaagcga ggattcaaaa
     4561 tggttagagg actgtacaca gcaggaacag gaatgctgac acagaggaat aaaataaatg
     4621 ttatagcgaa taatgtggcc aacgttgaaa cgacgggata caaggaagac ttgatgcttt
     4681 c
//
LOCUS       DAONVY010000133         4668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13239_length_4668_cov_3.525905, whole genome shotgun sequence.
ACCESSION   DAONVY010000133 DAONVY010000000
VERSION     DAONVY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13239_length_4668_cov_3.525905"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLI11_04745"
     CDS             <1..807
                     /locus_tag="PLI11_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52205.1"
                     /translation="FVFVELKSTEKELVTALREATLKHDMTDRISVITFHTSNITNMN
                     REFPEMSVGYLMGASATGATSDLQTRSVLGIIQPYGTTYNPSYNYHSKDFFTKANMRG
                     ITTWPWTINNEVVITYFLAGANGITTDTCQILAPFTKFLNAKKLEHKLEPGDNLKIEA
                     TRTTYGREEIDASEDVRIIFLEGEELVTVNDGVLTFKKYGTVVYALEYTHEIDENNSY
                     TVYSEPVTIAIEVLPGSSNTWLIIAIAAGVVVVAAAVILSIVFRKKKNNT"
     gene            824..1549
                     /locus_tag="PLI11_04750"
     CDS             824..1549
                     /locus_tag="PLI11_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52206.1"
                     /translation="MRTKLIVFILCIVLVLSLASCKKVGDDADKTPEVTPVVTAEPTA
                     TPEPTPSPEPTEKPTPPPTIEPGTGKYAEGILDENNGITFYLDKEDPAIIAGLHIDPA
                     SSASIQFFPTTTFDRIHVRLCTWTQQSGHKIEIALYRWMGSYDATLLSEPLFTKQYAD
                     YTDNIWLEVKFDQEYEDGEYLVDVVNISNANHVGIWISKEENDMQRFYHDGYVYEEGV
                     AQFSIGYTKTPNKKYGPLSDPGT"
     gene            complement(1595..>2159)
                     /locus_tag="PLI11_04755"
     CDS             complement(1595..>2159)
                     /locus_tag="PLI11_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ52207.1"
                     /translation="SYGISVGGNIKLTLTMLFGTPMDNVNDARTCMEIGGKKGYILSP
                     GCDMPFATPPENVKAITSYVHGNITEMFESEDVMAGVDYELPDYENEDKVIIDVITLD
                     SESCAPCQYTLEAIILAAEPIKDKVKIVEHKIKNKEGVACMLKLGASNIPTICVDGRI
                     EYVSILPDNNELTKCFMNRAIEKGLIS"
     assembly_gap    2160..2169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2170..2964)
                     /locus_tag="PLI11_04760"
     CDS             complement(<2170..2964)
                     /locus_tag="PLI11_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ52208.1"
                     /translation="MNKKQLVLDSIHNKETERVPWVPFVGCHAASLIGVTADEYFIDA
                     DLIVKGVKKAYEEYDPDGLPVLFDLQVEAEALGCELVYAKDNPPAVRTHVLEEGKELS
                     DLKIPTEKDGRFPIVLDATRRIVKELGDKIALYGLVTGPFTLALHLKGTDIFYDMLDD
                     PDEVHAVMEFCKEVCIKTSKMYMDAGVDIIALVDPMTSQISSDSFEEFVSPYATEIFD
                     FVRQSGKSSSFFVCGNAKRNIEAMCKTKPDNVSIDENIPLEYVLGIC"
     gene            complement(2976..3923)
                     /locus_tag="PLI11_04765"
     CDS             complement(2976..3923)
                     /locus_tag="PLI11_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPZ52209.1"
                     /translation="MKDKIDLYIVSGFLGSGKTTFLKNMLFNFDKKKTGVLVNEFGAV
                     NIDSVRLSGDTIKMVEINNGSIFCSCLKGDFIKALIAFSNEDIEYLFIENSGLADPSN
                     MRQILKEISEKTNKTYEYKGAICVVDATSFLKHVAVLPPVQNQVAHSSFIVLSKTDRV
                     NKEQITDVTNKLKEINPHAYVYETIYAEVPLSVLDEKLTVTTHEGETSNKPWNRISTY
                     ALETEEDVSIEELTRFVEEIKDFAYRIKGYVKDKKKWISVDVTELDVRINETSLTRRD
                     ILKHTRLVVISKSTECILDKIKETTKNTMPTKSIEIYEG"
     gene            4079..>4668
                     /locus_tag="PLI11_04770"
     CDS             4079..>4668
                     /locus_tag="PLI11_04770"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HPZ52210.1"
                     /translation="MSTHSIYEFVDREQVNEILESFYICIDLPIALLDKEGIRVKYYG
                     DTCRYCKTIKKYQPEGLCEKTYTRASDLAKNLGESYIFTCNGNLNHIILPLLNNGTVV
                     GSILAGPFLLDKPDSTLIQDIGKKYNIPTNAQFDMYDELSNVKIMPPTHVTHAARLLN
                     YLCHDLTEKKNKPNIKQIKLHQQARIGESIQMYKDT"
BASE COUNT         1362 a          964 c          908 g         1424 t
ORIGIN      
        1 tttgtatttg tagaattaaa gtccaccgag aaagaactgg taacggctct aagggaagca
       61 actttgaagc acgatatgac tgacagaata tcggtcatta ccttccatac gtcaaacata
      121 acaaacatga acagggagtt tcctgaaatg tctgttggat atttgatggg agcaagtgca
      181 acaggagcca ccagcgatct tcagacaaga agcgttctcg gcataataca accctatggc
      241 actacataca acccgtcata caattaccat tcaaaggact tcttcacaaa ggccaacatg
      301 agaggcatca ctacatggcc atggacgatt aacaatgagg ttgtgattac atatttcctt
      361 gctggagcca acggtatcac aaccgacaca tgtcagatac ttgcaccctt tacaaagttc
      421 ttgaatgcaa agaaattaga gcataaatta gagccgggcg acaatcttaa gattgaagcc
      481 acaagaacca cgtatggcag ggaagaaatt gatgcatcag aagatgtaag gataatcttc
      541 ctggaaggag aagaacttgt tactgtgaat gacggagtcc ttacatttaa gaaatacgga
      601 actgttgtat atgcccttga gtatactcat gagattgatg aaaacaattc atatacggta
      661 tattcggaac ctgtaaccat agctattgaa gtattgccag ggtcttcaaa tacatggttg
      721 ataattgcaa tcgctgcagg tgtggtagtt gttgcagctg ctgtgattct ttccatagta
      781 tttagaaaga agaaaaataa tacttaaact ggggggttga tttatgcgta ccaagttaat
      841 tgtgtttatt ctatgtattg ttctggtttt gtcactggct tcctgtaaaa aggttggaga
      901 cgatgcggac aaaactccgg aagtcacacc ggttgtaacg gcagaaccta ctgcaactcc
      961 ggaaccgaca cctagtcctg agcctacgga gaagccaacg ccaccaccta caatagagcc
     1021 aggaacaggc aagtatgcag aaggaatttt ggatgaaaac aatggaataa cattttatct
     1081 ggacaaggaa gatcctgcaa tcattgccgg acttcacatt gatccggctt cctctgcgag
     1141 cattcagttt ttcccgacca ccacatttga ccgaattcat gtcagattgt gtacctggac
     1201 acagcagagc ggtcataaga tagagatagc cctgtacaga tggatgggaa gctatgatgc
     1261 gacccttctg tcagaaccgc tttttacaaa acagtatgca gactatactg acaatatatg
     1321 gctggaagtt aaatttgatc aggagtatga ggatggcgaa tatctggtgg atgttgtgaa
     1381 catatctaat gcaaatcatg tgggtatttg gatatcaaag gaggaaaacg acatgcagag
     1441 gttctatcat gatgggtatg tatacgagga aggtgtggca cagttttcaa tcggatacac
     1501 aaaaacacct aacaaaaaat atggaccatt atcagatccg ggaacataaa aagagaggcg
     1561 gaaccgcctc tcttttacca aaagaagtga tatattaact gataagtcct ttttctatag
     1621 ccctgttcat aaaacatttg gtaagttcat tgttgtcggg caagatactt acatattcaa
     1681 tcctgccgtc aacgcaaatg gtcggaatat tgcttgctcc aagtttcaac atgcaggcaa
     1741 caccttcctt gttcttaatc ttgtgctcaa caattttgac cttgtcttta ataggctctg
     1801 ctgccaatat gattgcctca agggtgtact gacatggtgc acagctttct gaatcgaggg
     1861 taattacatc aattatcacc ttatcttcat tctcgtaatc aggaagctcg taatccacgc
     1921 ccgccataac atcctcgctt tcaaacatct cggtaatgtt gccgtgaacg tagcttgtta
     1981 ttgcttttac attctcagga ggtgtagcaa acggcatgtc gcaaccgggt gaaagtatgt
     2041 atcctttctt tcctccgatt tccatgcatg ttcttgcatc atttacgtta tccataggag
     2101 tgccgaatag cattgtcagc gtaagcttta tatttcctcc aacggaaatg ccgtaggacn
     2161 nnnnnnnnng caaatgccaa gaacatattc aagaggtatg ttttcatcta ttgaaacatt
     2221 gtcaggtttg gttttgcaca ttgcttctat gttccttttt gcatttccgc acacaaagaa
     2281 gctggagctt ttgcctgact gtcttacaaa gtcaaatatt tccgttgcgt atggagatac
     2341 aaattcctca aagctatcgg aagaaatctg actggtcatg ggatcaacca aagctataat
     2401 gtcaacgcct gcatccatat acattttaga agttttgatg cacacttctt tacagaattc
     2461 cattacggca tgaacttcat ccggatcatc cagcatgtcg tagaagatat ccgtgccttt
     2521 caagtgaaga gccagtgtga atggtcctgt cacaaggccg taaagagcta tcttgtcccc
     2581 cagttccttt actattctcc ttgttgcgtc caatacgatg ggaaatctgc catccttctc
     2641 tgtaggaatt tttagatcgg acagttcttt gccttcttcc aggacgtgag tccttactgc
     2701 aggtggattg tctttggcgt aaaccagttc acagcccaaa gcctccgcct ccacctgcag
     2761 gtcaaaaaga actggcagac cgtccggatc atattcctcg tacgctttct taacaccctt
     2821 tacaatcaaa tctgcatcaa tgaaatattc atctgcggtt acaccaatca gacttgccgc
     2881 atggcatccg acaaaaggca cccatggcac tctttcggtt tccttgttat gtatgctgtc
     2941 cagcacaagt tgttttttat tcattttcta ctctcctaac cttcatatat ctcaatgctt
     3001 ttcgttggca ttgtattttt tgttgtttct ttaattttat ctaaaataca ctcagtactt
     3061 tttgatatga caaccaatct tgtatgtttt aggatatctc ttctggttaa agatgtttcg
     3121 ttgattctga catccaattc ggtaacatcc actgaaatcc attttttctt atcctttaca
     3181 taacctttta ttctgtatgc aaaatccttt atttcttcaa caaacctggt taattcctca
     3241 atggatacgt cttcctcagt ttcaagcgca tatgtgctta ttctgttcca tggcttgttt
     3301 gatgtttctc cttcatgggt tgtaactgtc agcttctcat ccaaaacgga aagagggact
     3361 tccgcataga tggtctcata cacgtatgca tgagggttta tctcctttag tttattcgta
     3421 acatcagtta tttgctcttt gttaactctg tcggttttgc tcaaaacaat gaagcttgaa
     3481 tgggcaacct ggttttgaac gggaggaaga acagctacat gtttcaaaaa ggatgtagca
     3541 tcaacaacac atatggctcc tttgtactcg tatgtcttgt ttgtcttttc gctgatttcc
     3601 ttaagtattt gtctcatgtt tgaagggtct gcaagacctg agttttcaat aaataagtat
     3661 tcaatgtcct cattcgaaaa ggctatgagc gcttttataa aatcgccttt taagcagctg
     3721 cagaatatgg agccgttgtt tatctccacc atttttatgg tgtcaccgga caaacgtaca
     3781 gagtcaatat ttactgctcc gaactcattt acaagaactc ctgttttctt tttatcaaaa
     3841 ttaaaaagca tgtttttcag aaaggtggtc ttgccacttc ccaaaaaacc ggatactatg
     3901 tacagatcta ttttgtcttt cataaaatca ccttaacctt tgcagagtat attatattct
     3961 ttgggcattc tggctttttg tattattttt ttatttatat aacgatttta tgaatgtagt
     4021 ttttttgtaa tattttatga tataatatat tcattaaaat gtactggagg acgaaagcat
     4081 gtcaacccat tcaatttacg aattcgtgga ccgggagcag gttaacgaaa tacttgaatc
     4141 cttttacata tgcatagatc ttcccatagc ccttcttgac aaggaaggga ttcgggtaaa
     4201 gtattatgga gacacatgca gatactgtaa gaccataaaa aagtatcaac ctgagggttt
     4261 gtgcgaaaaa acctacactc gtgcaagcga tctcgcaaaa aatctgggag agtcatatat
     4321 ctttacctgc aatggcaacc ttaatcatat aatacttcct cttttgaaca atggaactgt
     4381 tgtaggttca atacttgcag ggcccttttt gcttgacaag cctgattcca cattgataca
     4441 ggatattggg aagaaataca atatcccaac gaatgcgcaa tttgacatgt atgacgagct
     4501 gtcgaatgta aaaattatgc ctcctacgca tgttacccat gctgcaagac ttctaaatta
     4561 tttgtgtcac gatcttactg aaaagaaaaa caaaccaaat atcaagcaga taaaactgca
     4621 ccaacaggca agaattggcg aatccataca aatgtataag gatacgga
//
LOCUS       DAONVY010000134         4662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13259_length_4662_cov_3.494248, whole genome shotgun sequence.
ACCESSION   DAONVY010000134 DAONVY010000000
VERSION     DAONVY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13259_length_4662_cov_3.494248"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLI11_04775"
     CDS             complement(<1..277)
                     /locus_tag="PLI11_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPZ52211.1"
                     /translation="MRLTKDNIVVRSATVEDAILLNQWWNDGRAMEHAGFPYGVGESL
                     EETIKNIKSRKDESGQLCIIEIDGKAVGELSYRIEDNTAYPGWKICDF"
     gene            complement(290..733)
                     /gene="rlmH"
                     /locus_tag="PLI11_04780"
     CDS             complement(290..733)
                     /gene="rlmH"
                     /locus_tag="PLI11_04780"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009428725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPZ52212.1"
                     /translation="MTISIVAVGKIKEKYMTQAINDYVKRLGRYGKVEIVEVNEETSV
                     EKEGENLLNRIPKGSYVIVLDLYGKEIDSVEFAQVFEKAQINGYSRFSFVIGGSEGIS
                     DEVRKQADMLLCLSKMTFTHQMARFIFLEQIYRAMKINNNEPYHK"
     gene            complement(699..1304)
                     /locus_tag="PLI11_04785"
     CDS             complement(699..1304)
                     /locus_tag="PLI11_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPZ52213.1"
                     /translation="MAAGEDARRFASKINQEALRLLTLIGDILKISELEELKEEKQFD
                     KVDLEDVIRECVDNLKLQAESKNVTIKTNITKPAFIQGKKDLAEVIVYNLCDNAIRYN
                     VENGMVIITFKDNVLSVKDTGIGIPKEYHSRIFERFFRVDKSRSKETGGTGLGLAIVK
                     HAAKRLDATIEFESEPRKGTEIKIYFKGWENDNKHSSGRQN"
     gene            complement(1313..2416)
                     /locus_tag="PLI11_04790"
     CDS             complement(1313..2416)
                     /locus_tag="PLI11_04790"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPZ52214.1"
                     /translation="MKRKIYTRLLLIGVLSIIFTNLCTMLVYYGMFKKQMKEDVRTTA
                     VLLSSLYNQTEDVKSNVDLLDIDGRVTLISEDGTVLYDNQADVNAMENHKNRPEVKMA
                     LEKGIGQSVHTSRTMGTNAYYCAVKLDDGNVIRYSVNARSAVAVFVGSLPVLLLISIV
                     IFFICLSYAKVMTRQVIKPINDISSRLAEDDVEHIDSPYEELLPFVKAIKERNERIAN
                     EIAKLQFEKNKIEAVMENMSEGMILLDEKMRVVTVNNSAVKMLDGNMNCVGRNILFFS
                     RNETLSSIAKDAISGHKVSKDIYSKGKWFQVIGSPVDNEGVRIGAILLILDITEKKET
                     ETIRREFTANVSHELKTPLTSITGYSELIESNS"
     gene            complement(2418..>2624)
                     /locus_tag="PLI11_04795"
     CDS             complement(2418..>2624)
                     /locus_tag="PLI11_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPZ52215.1"
                     /translation="KEYELLKYLIMNHGIVVSRDKLLENVWGYDFAGGSRTVDMHIKS
                     LRQKLGECASLIKTVRNVGYKLGE"
     assembly_gap    2625..2634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2635..3087)
                     /locus_tag="PLI11_04800"
     CDS             complement(<2635..3087)
                     /locus_tag="PLI11_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPZ52216.1"
                     /translation="MIYIVEDDENIRELESYTLRSNGFEVREFEDGESFLKACREKVP
                     GLVILDIMLPENDGLSILKEIRQNVATKDVPVILITAKGFEMDKVRGLDMGADDYLTK
                     PFSVLEMVSRVKALLRRAGGSGVEDSALKYDDIEIDDAARVVRVRGELV"
     gene            complement(3102..3743)
                     /gene="phoU"
                     /locus_tag="PLI11_04805"
     CDS             complement(3102..3743)
                     /gene="phoU"
                     /locus_tag="PLI11_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPZ52217.1"
                     /translation="MRSRFDEQLDALNKELVEMGALIEKAIDIAVKALLEQNEELAKF
                     AVLFDNEVDQKEKDIENMCLKLLLQQQPVAKDLRLISAALKMITDMERIGDQAADISE
                     ITLMMLDKPYIKKLEHIQQMAEATTKMVKESIDAFVKMDYDLAKSVVDYDDVVDDLFN
                     VVKKEVVELIRDDISNSEQSVNLLMIAKYFERIGDHATNIAEWVIFAIKGIHV"
     gene            complement(3751..4506)
                     /gene="pstB"
                     /locus_tag="PLI11_04810"
     CDS             complement(3751..4506)
                     /gene="pstB"
                     /locus_tag="PLI11_04810"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPZ52218.1"
                     /translation="MSKFKFEIKNLDVFYGNFQALKNVSMNIDENEITAFIGPSGCGK
                     STLLKSLNRMNDLTEGCKTKGEVFLDGENIYSDIDVYDLRKRVGMVFQKPNPFPMSVY
                     DNIAYGPRTHGIRSKSRLDEIVEESLRAAAIWDEVKDRLKKNALGFSGGQQQRLCIAR
                     ALAVKPGVLLMDEPTSALDPISTAKIEELVTELKKEYTIIIVTHNMQQALRISDKTAF
                     FLLGELIEYSKTDDLFSVPKDQRTEDYITGRFG"
     gene            complement(4511..>4662)
                     /locus_tag="PLI11_04815"
     CDS             complement(4511..>4662)
                     /locus_tag="PLI11_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HPZ52219.1"
                     /translation="GRTLSVHMYALSSEGLYTNEAYATAVILLVFVLAVNGLSNLAAR
                     KLRKG"
BASE COUNT         1217 a         1198 c          693 g         1544 t
ORIGIN      
        1 caaaatcaca tatcttccac ccgggatatg cagtattatc ctcaattctg taacttagct
       61 cgccaacagc ctttccatct atctcaatga tgcaaagctg ccctgattca tcttttctgc
      121 tctttatgtt ctttatggtt tcctcaagag actctcccac tccataggga aatcctgcgt
      181 gttccatggc acgcccgtca ttccaccact ggttcagaag aattgcatct tcaactgtag
      241 cacttcttac aacaatattg tcttttgtca gcctcataac taccctcttt tacttatggt
      301 acggttcgtt gttattgatc ttcatcgccc tgtatatctg ctcaagaaag ataaaccttg
      361 ccatctggtg ggtaaatgtc atttttgaca ggcaaagaag catatcggcc tgctttcgca
      421 cttcatccga tattccttca gatccaccaa taacgaatga aaaacgagag tagccgttaa
      481 tttgtgcttt ttcaaacact tgagcaaact ccactgaatc tatctcttta ccgtatagat
      541 caaggacgat aacataagaa ccctttggta ttctgtttaa aagattttct ccttcttttt
      601 caacagaagt ctcctcattg acctcgacaa tctcaacttt accgtatctt ccaagccttt
      661 ttacataatc atttattgcc tgggtcatgt acttttcctt aattttgcct accgctacta
      721 tgcttattgt cattttccca tcctttgaaa tatattttta tctctgtacc ttttcgtggt
      781 tcactctcaa actcaattgt tgcatcaagt ctctttgctg catgctttac aatcgcaaga
      841 ccaagacctg ttccacctgt ttccttggac ctgctcttgt ccactctgaa aaacctttca
      901 aaaatccttg agtgatactc tttaggaatc cctatgcctg tatccttgac tgaaagaaca
      961 ttgtctttaa acgtaattat taccatcccg ttctccacat tgtatcttat tgcgttgtca
     1021 cacaagttgt atacaataac ttccgcaaga tccttcttgc cttgaataaa tgctggtttt
     1081 gtaatatttg ttttgattgt cacattcttg ctctctgcct ggagctttaa attatcaaca
     1141 cattccctta tcacatcttc caaatccacc ttgtcaaact gcttctcctc tttcagttcc
     1201 tccagctccg atattttaag tatatcgcca attaaagtca acaatcttaa agcttcctga
     1261 ttaatcttgc ttgcaaaacg ccttgcatcc tctcctgcag ccatcccgct ttctatgagt
     1321 tgctttctat gagttcgctg tacccggtta tactggtaag aggagtttta agttcatgtg
     1381 agacatttgc agtaaactct cttctgatag tttctgtttc cttcttctca gttatgtcaa
     1441 gtataagaag gattgcacca attcttacac cttcattgtc aacagggctt ccaataacct
     1501 gaaaccattt gcctttggaa taaatatctt ttgacacctt gtgtccgctt attgcatcct
     1561 ttgcaatact tgaaagggtt tcatttcttg aaaagaacaa tatgtttctg ccaacacaat
     1621 tcatattgcc atccagcatt ttaaccgcac tgttattcac ggttacaaca cgcattttct
     1681 catcaagtag aatcatgcct tctgacatgt tctccataac cgcttcaatt ttattcttct
     1741 caaactgcag ttttgcaatc tcattagcta tgcgttcatt tcgttccttg atcgccttta
     1801 caaacggcaa cagctcctca tacggagaat caatatgttc cacgtcatct tccgcaagtc
     1861 tgcttgatat atcgtttata ggctttataa cctgccttgt catgactttt gcataggaca
     1921 agcatataaa gaagatgaca atagatatca atagcaaaac aggcagcgag cctacaaaaa
     1981 cagccactgc acttctagca tttaccgaat accgtataac attcccatca tctaatttga
     2041 ccgcacagta atatgcattt gttcccatgg ttctggatgt atgtacggac tgaccgatac
     2101 ctttttccaa agccattttg acctccggtc tgtttttatg attctccatg gcatttacat
     2161 ctgcctggtt gtcatacaaa acagtgccgt cttcagaaat caaggttact ctgccgtcaa
     2221 tatcaagcaa atcaacattg ctttttacat cctcggtttg attgtacaaa ctggatagca
     2281 aaacagcagt tgtacgcaca tcctctttca tctgtttttt aaacatgccg tagtagacaa
     2341 gcatggtaca caagtttgtg aatataattg aaaggacacc gatcaaaaga agcctggtat
     2401 atatttttct tttcatgtca ttctcccagt ttgtatccta cattcctgac agtctttatc
     2461 aaacttgcac actctcccag cttctgcctg aggcttttga tgtgcatgtc cacggttctg
     2521 cttcctccgg caaaatcata cccccataca ttttcaagaa gtttgtctct tgatacaaca
     2581 atcccatgat tcattatcag gtatttgaga agttcatact ccttnnnnnn nnnncaccaa
     2641 ttcacccctt actctcacta ctcgggcagc atcatcaatc tcaatgtcat catattttaa
     2701 cgcgctgtcc tctacgccag atcccccagc ccgccttaaa agagccttta cgcgcgacac
     2761 catttccaga acagaaaagg gtttggtcaa gtaatcgtcc gcccccatgt caagacccct
     2821 taccttgtcc atctcgaaac cttttgcggt tatgagaatg acaggcacat ccttggtagc
     2881 cacattctgt ctaatctctt ttagtattga aagtccatca ttctcaggaa gcattatatc
     2941 caaaatcaca agaccaggta ctttttccct gcatgctttc aagaaggatt ctccgtcttc
     3001 aaactcccgc acttcaaaac cgttgcttcg caaagtgtag ctctccagct ccctgatgtt
     3061 ttcatcatcc tcgacaatgt atatcatttg atacctccag actatacgtg tatgcctttt
     3121 atggcgaata taacccattc tgcaatattt gtggcatggt cgcctatcct ttcaaaatac
     3181 ttggcaatca taagaagatt tacagactgc tcactgttac taatatcatc cctgatcagt
     3241 tcaactactt ccttctttac cacattgaac aaatcatcca ccacatcatc ataatccacc
     3301 acactctttg ccaaatcgta atccatcttt acaaatgcat caatgctttc cttcaccatc
     3361 ttggttgttg cttcagccat ttgctgtata tgttcaagtt tcttgatata tggcttgtca
     3421 agcatcatca aagttatctc tgaaatgtcc gccgcctggt ctccaatcct ctccatatcg
     3481 gtaatcatct ttaaagctgc ggatatgagc ctaagatctt ttgccactgg ctgctgctga
     3541 agaagtagct tgagacacat attctctata tccttctcct tctgatctac ttcattatca
     3601 aataaaaccg caaattttgc aagttcctca ttctgttcca aaagagcttt aaccgcaata
     3661 tcaattgctt tttcaatcaa tgctcccatc tctacaagtt ccttgttaag agcatccaat
     3721 tgttcatcaa atctgcttct caatctcata tcatccaaac ctcccggtaa tatagtcttc
     3781 cgtgcgctga tctttcggta ccgaaaacag atcatccgtc tttgaatatt ctattagttc
     3841 acccagcaaa aagaaggctg ttttgtccga aatcctgaga gcctgctgca tattgtgcgt
     3901 tacaatgatt atagtatatt cttttttaag ttcggtaaca agctcttcta tctttgccgt
     3961 ggaaattgga tccaatgcac tcgttggctc atccataagc aagactccag gttttaccgc
     4021 caaggctctt gctatgcaga gcctctgctg ctgcccgccc gaaaagccta gagcattctt
     4081 tttcaacctg tccttgacct catcccaaat tgccgcagct ctcaaagact cttccacaat
     4141 ttcatccagc cttgattttg agcgtattcc gtgggttctt ggcccatacg caatattgtc
     4201 gtaaacactc atagggaacg gatttggctt ttgaaacacc attccaaccc tctttctcaa
     4261 atcataaaca tcaatatcgc tatatatatt ctctccgtca agaaaaacct ctccttttgt
     4321 tttgcagcct tctgtcaaat cattcattct gttaaggctt ttaagcaagg tggacttgcc
     4381 gcaacccgac ggtccaataa aagctgttat ctcattttca tctatgttca tcgacacatt
     4441 cttcaatgcc tgaaagtttc cgtaaaatac atcaagattc tttatctcaa atttaaattt
     4501 actcatacca tcaccctttc cttagttttc ttgctgccag gtttgacagt ccatttacag
     4561 ccaacacaaa cacaagtagt attactgcag ttgcatatgc ctcattcgtg tacaacccct
     4621 cactggacag ggcatacatg tgaaccgaca aggttcttcc tg
//
LOCUS       DAONVY010000135         4649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13300_length_4649_cov_3.323465, whole genome shotgun sequence.
ACCESSION   DAONVY010000135 DAONVY010000000
VERSION     DAONVY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13300_length_4649_cov_3.323465"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLI11_04820"
     CDS             <1..218
                     /locus_tag="PLI11_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52220.1"
                     /translation="DESGFIIRAFDITGDGGEAFIQINADFEVTAVDTLERPIDKEVS
                     RDGSRVLVNLSPCETIALKVTLNKGVV"
     gene            220..414
                     /locus_tag="PLI11_04825"
     CDS             220..414
                     /locus_tag="PLI11_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HPZ52221.1"
                     /translation="MSFPGVVFVLVISVVALAINFLPSFIAFIRKHPNRIPILVVNAL
                     IPILGFLIAFLWTFADVQTE"
     gene            434..649
                     /locus_tag="PLI11_04830"
     CDS             434..649
                     /locus_tag="PLI11_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018931362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HPZ52222.1"
                     /translation="MILTNIYQVLCSLLGTLVVILTAVINFIPSIIAFKKNHPDKVMI
                     LIINALIPVAGFIIALILVFVRKEDRK"
     gene            complement(651..1523)
                     /gene="lipA"
                     /locus_tag="PLI11_04835"
     CDS             complement(651..1523)
                     /gene="lipA"
                     /locus_tag="PLI11_04835"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPZ52223.1"
                     /translation="MKEFVRKPSWLKVKSNTGEENQRVMNMLRDLNLHTVCEEAGCPN
                     CGECFGRKTATFMILGNKCTRNCRFCNVTKALPCEVDENEPVNIAMAVKNLGLKHVVV
                     TSVSRDDLVDGGSGHFAKVIHAIREKVGDSVIVEVLIPDFGGQLESLKTVIDARPDII
                     NHNVETVPRLYPMVRPMADYQRSLELVGRVKEHSEGIVTKSGIMVGHGETFEEVVDVF
                     KNLREVDCDILTIGQYLSPSKEHLPVYEYVHPDLFEEYKKRALELGFRYVASGPLVRS
                     SYMAEEAYKEIKDS"
     gene            complement(1520..2188)
                     /gene="lipB"
                     /locus_tag="PLI11_04840"
     CDS             complement(1520..2188)
                     /gene="lipB"
                     /locus_tag="PLI11_04840"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPZ52224.1"
                     /translation="MKVNLCMPGRMDYEKALELQYTLQERRMNNEMDDTLLLLEHPPV
                     ITLGTRGKSDNIYLSEEELKLLGIKVVKVNRGGDVTYHGPGQIVGYPIFGLNGFGKDI
                     RWFVYRLEKFIIELLKEEFEIDAYRDEGRFTGVWVGDKKIMAIGIAVKRWVTMHGFAF
                     NVNTDLSHFAWINPCGLNKGVVSLKCLTGVSQDMNRMFEYVRDYFSKEFDCDFVEKKV
                     EELI"
     gene            2336..3184
                     /locus_tag="PLI11_04845"
     CDS             2336..3184
                     /locus_tag="PLI11_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52225.1"
                     /translation="MDLNKVIDYIEKNLTNDITLADICKVASCSDYALRNVFYYLTGL
                     TLSEYIKYRKLSEANKDLLNNEKVTDVAYKYGYESVDGFSRAFKAWSGLLPSEAAKLN
                     ICKTLPKLTFNIDVKGGNSMDYRIVEMPGFFIAGVSKRVPMKFEGVNNAIVELAQSIT
                     QEQREEMHTLKNIEPFEVVNASYEHDYDFQKDAGELTHMIGVLTTKENISSNLDKVRI
                     PACTWAVFPNKGPFPETLQNTYARIYSEWLPVSGYELEKAPMFSFSKMDNEQAYSEIW
                     VAVKKA"
     gene            complement(3293..3502)
                     /locus_tag="PLI11_04850"
     CDS             complement(3293..3502)
                     /locus_tag="PLI11_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52226.1"
                     /translation="MDKYDFEKAEKEMQELWDEYDVYHFEIDNPGINERAKRIYQKSF
                     VRLKGKRRNVMLFSEKGMLLKFEPI"
     misc_binding    complement(3563..3824)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3990..4364)
                     /locus_tag="PLI11_04855"
     CDS             complement(3990..4364)
                     /locus_tag="PLI11_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52227.1"
                     /translation="MSVETLGLRKLQEIICSISNVDELENEDIIYLTKWIESNSKLAG
                     NYLYDKICGLLNYMVNDDEMLYELKRFVCPVHNSGISGDIDLENKTVCLTGNFELGSK
                     EQVEELLTSMGATCKNSVNKKY"
BASE COUNT         1426 a         1067 c          740 g         1416 t
ORIGIN      
        1 aagatgaaag cggctttatc ataagagctt tcgacataac aggagatggc ggcgaggctt
       61 tcatacagat aaatgcagat tttgaagtaa cggcggtgga tactcttgaa agacctatag
      121 acaaggaagt atcaagagat ggcagcaggg ttttggtaaa cctaagcccc tgcgagacaa
      181 ttgctctgaa agtaacttta aacaaaggag ttgtttagca tgtcttttcc aggtgttgtt
      241 tttgtgttgg tgatctcggt tgtggcactg gctataaatt ttcttccatc atttatagct
      301 ttcataagaa aacaccctaa ccgaatccca atacttgtag taaacgcact gattccaata
      361 cttggttttt tgattgcatt tttatggacg tttgctgatg tacaaacgga ataataatta
      421 aggagatgat gatttgatac ttaccaacat ttatcaagta ctgtgcagtt tgcttggcac
      481 attagttgtc atattgactg cagttataaa tttcataccc agcatcattg cctttaagaa
      541 aaaccatcct gacaaggtaa tgatactcat aatcaatgcg ttgatacctg ttgcaggctt
      601 cataattgca ttgatactgg tttttgtaag aaaggaagac aggaaataaa tcagctatcc
      661 tttatttcct tgtacgcttc ctccgccatg tatgaacttc gtaccaaggg tcctgaggct
      721 acatacctaa acccaagctc cagggctctt tttttatact cctcaaacaa atcaggatga
      781 acatactcat aaaccggaag atgctccttt gacggcgaca gatactgacc tatggttaaa
      841 atgtcacagt caacctctct caaattctta aaaacatcta caacttcttc aaaagtctcc
      901 ccatggccca ccatgatacc tgacttggtc acaataccct cggaatgctc ttttactctt
      961 ccaacaagct ccaacgaacg ctggtaatca gccatgggtc ttaccatggg atacagcctg
     1021 ggtaccgtct ccacattgtg atttataata tcggggcggg catcaattac agtctttaat
     1081 gactccagtt ggccgccaaa atccggaatc agcacttcaa caattacgct atcacccacc
     1141 ttttccctta tggcatgaat gactttggca aaatgaccgc tgccgccatc cacaaggtca
     1201 tcccttgaaa ccgaagtaac aaccacatgt ttaagaccca gatttttcac agccattgct
     1261 atgttaacag gctcattctc atcaacctcg caaggtaatg cctttgtaac attgcaaaac
     1321 ctgcagtttc ttgtacactt gtttccaaga atcatgaatg tggctgtttt cctcccaaaa
     1381 cactcaccac aattgggaca gcctgcttct tcacatacgg tatgaagatt aagatcccgc
     1441 agcatgttca tgactctttg gttttcctct cccgtattag actttacttt cagccatgac
     1501 ggctttctta caaactcttt catattagct cctcaacttt cttctcaaca aaatcacaat
     1561 caaactcttt tgaaaaataa tcccttacgt attcaaacat tctattcata tcctgactga
     1621 cacctgtaag acattttagt gaaacaacac ctttgtttag cccgcaaggg tttatccatg
     1681 caaaatgaga caaatccgta ttcacattaa aagcaaatcc atgcattgtt acccatctct
     1741 tgacagctat gccaattgcc atgatttttt tgtctcctac ccacactcca gtaaacctgc
     1801 cctcatccct atacgcatct atttcaaact cttcttttag cagctcaata atgaactttt
     1861 caagcctgta tacaaaccat cttatgtcct tgccaaaacc attcaaacca aaaataggat
     1921 accctacaat ctgtccagga ccgtgatatg taacatcccc tcccctgtta accttgacca
     1981 cctttatacc tagaagcttc agctcttctt ctgaaagata tatattgtcg gatttgcccc
     2041 ttgtccctaa ggttataacc gggggatgtt caagaagcaa taaagtatca tccatctcat
     2101 tgttcattct cctttcctgc agcgtatact gcagctccaa agccttttca taatccattc
     2161 tgcccggcat gcatagattt accttcaatt ctgccatcac ctttgttttt tttagagaaa
     2221 actatatcat gacacaaaac caaaagtcaa atctaatacg acaaaacttg ttttaaaatg
     2281 tcgagtatta caccctgcac tctggtataa tcactaatga gaggagtgaa aattaatgga
     2341 cctcaacaaa gttattgatt atattgaaaa aaaccttaca aacgacataa cattagccga
     2401 tatatgcaaa gttgcaagct gctcagacta tgctttgcgc aatgtctttt attaccttac
     2461 cggtttgact ctgagtgagt atataaaata cagaaaactc tcagaagcaa acaaggatct
     2521 gttaaacaac gaaaaagtta cagatgtggc atataaatac ggttatgagt cagtggatgg
     2581 gtttagccgg gctttcaaag cctggagtgg attgcttcca tcagaagctg caaaactaaa
     2641 catatgcaaa accttgccaa aactgacttt taatatagat gtaaaaggag gaaatagtat
     2701 ggattatcga atagttgaaa tgcccggatt tttcatagcc ggcgttagca aaagagttcc
     2761 catgaaattt gagggtgtga acaacgcaat agtggaactt gcacaaagca taacccagga
     2821 acaaagagag gaaatgcata cccttaagaa tattgagcct tttgaagttg taaacgcatc
     2881 atatgagcat gattacgact tccaaaagga tgcaggcgaa ctcacccaca tgattggagt
     2941 tttgacaaca aaggaaaaca taagcagcaa tcttgataaa gtcagaatcc ctgcctgcac
     3001 ctgggctgtc tttcccaaca agggaccttt cccggaaacc ttgcagaaca cctatgctag
     3061 aatctactcg gaatggctgc ctgtatcagg ttatgaactg gaaaaggcgc ctatgttctc
     3121 tttttccaaa atggacaatg aacaggcata tagcgagatt tgggttgcag tgaaaaaggc
     3181 ataagtgcat aaagcttatg ccttttcaac aactaacaac aatccatttg tttaccggca
     3241 tcagcccaac ttcctcatca tgctcaaaat ccatcaaatg catgattaaa aatcatatgg
     3301 gttcgaattt aagaagcatt cctttttctg aaaacagcat tacatttctt ctcttgcctt
     3361 taagccttac aaatgatttt tgatatattc tctttgccct ttcatttata cccggattgt
     3421 caatttcaaa gtgatacacg tcatattcat cccataactc ctgcatttcc ttttcagcct
     3481 tttcaaaatc atatttgtcc atttcaacac ctccagaata ataccagaat aatatataaa
     3541 aaaagtccgc cttatgcatt atgcttaggg cgaaccaata atctgcggta ccacctaaat
     3601 tcaagtataa acacatctgc actctgccaa tacgggataa ctaaagtcat ccgatattgc
     3661 ttccctttta acggtgggaa tttccgttgg agcctactaa gaccaaagtc cgttcagtcc
     3721 acagctcaaa ggctatttca acacttccct ctcgaagatt tgcaccaccc atcttctctc
     3781 tacgcttcaa gacagtatct acttctcctt atcaacgcca ttttgtaatt taaatttaaa
     3841 acatctctaa accctatttg aaaatcccat tttcatgaac aattattata ttgcacccct
     3901 tttcattcaa ctccattgct ttcattactt ttgagccata cgtaccatag ctccaggctt
     3961 tactgccaag tccacctacg atcacgatat cagtactttt tattaacact gttcttacat
     4021 gtagcaccca tacttgtaag taactcctca acttgttctt tcgagcccaa ttcaaagttt
     4081 ccagtcagac acacagtttt attctctaaa tctatatctc cagaaattcc agaattatga
     4141 acaggacaca caaatctttt caactcgtat aacatttcat catcatttac catataatta
     4201 agcagtccac atattttgtc atacaaataa ttgcctgcaa gtttactatt cgattcaatc
     4261 cacttggtta gataaataat atcctcattc tcaagttcat ctacattgga tattgagcag
     4321 ataatctctt gaagctttcg aagtccaagg gtttcaacgc tcaaatttat ttcgccatct
     4381 tggtcctttt ttagagttgc tatcagtaaa ataattcttg agttccatat gtttcatttt
     4441 ttcataacat tcataacatt tatatgtcgt tatgcaatca tccaatgcgc gatgtttgaa
     4501 cttgacttgt actcgcaggt aatctgcaat ggaagacaat gtataacttt cctgtccatg
     4561 gatgcaacat cttgccagat acattgtgtc tacaaaatcg ttggataaaa catgtccata
     4621 tagatttaac gaagcgttat taagaatat
//
LOCUS       DAONVY010000136         4642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13315_length_4642_cov_3.568563, whole genome shotgun sequence.
ACCESSION   DAONVY010000136 DAONVY010000000
VERSION     DAONVY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13315_length_4642_cov_3.568563"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLI11_04860"
     CDS             <1..1208
                     /locus_tag="PLI11_04860"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPZ52228.1"
                     /translation="IEADIRRKNFQEYVPIYTEEEAKREAMRCLECGCMDYFECKLID
                     YANKYGVAPERFAGDVHNRRKPSTHLHIVRDSDKCILCGLCVRLCEEVRGVTALGLVG
                     RGFDTIVEPEMRKVLEETECDSCGLCVSACPTGALVEKQSLIKAVPLNEKYTVSTCGL
                     CSKACSIKVATYGNHVVRVLPVSDARTADILCIKGRFMYVDVLSNDRLKSAYINDREC
                     DVEKACEYIIEEIRKNTGKKVVFVSADYTNEQVDKVLLFAKAIGAEVVSGILRQHKFD
                     DASAFAGVNEFGLRVKGIKALEDAANLKEEIENGDVEVLVSFGDKLEGINLDNVNFKA
                     FMSLHMPKHEGVNVLLPDSSYFEIIGTFTKKSEDGTFEDREVNACIKPFGGISSSEIL
                     DNLCGKVCE"
     gene            1208..1714
                     /locus_tag="PLI11_04865"
     CDS             1208..1714
                     /locus_tag="PLI11_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HPZ52229.1"
                     /translation="MRNKVMYITTAAMIAAIYTALTLAGVGFAYGPVQFRFSEALSIL
                     PVFTPAAIPGLTIGCVIANLIGPYGIADVVLGSIATLLSALFAYATRNIRVWKLPLLA
                     PLGAVVFNAFIVSAIIFFFTPEDVAYFISVIWIGLGQLASCYGLGIPLFYILDKSKLF
                     DRFNGYTH"
     gene            1824..>4642
                     /locus_tag="PLI11_04870"
     CDS             1824..>4642
                     /locus_tag="PLI11_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52230.1"
                     /translation="MIFKEVLGRDSFIKRAEMAAHSHHIKGKCKKWPRHLPKPQELKD
                     VIENTYRRIASRYIEERSVAMPAEEWIADNYHFIKGQLNTFEDLGKQIKYLPMIERSD
                     IKGYSELRIYSICSEIVEYTDGLVDEGAISDYLDSYQKISPLKTAELAVIAPMLRLAL
                     LNRIALVCKMTDRVTEERHESELMFNRYVNYIEGKGASNRALEKWISSVGDLSPVFAE
                     TILRLAAKWSDDTEQFRNILKRKLAGRGITLDELIAKEHKTRSALGVSVGNAITGLNN
                     LTSIHWSALLKKLSIVEQILLKDPVDVFSKMGRQSRTYYVYLIDVYARRMGVSAESVA
                     MTAIELASASSSPQHVGYYLYKEGYRDLKKAVSEKFKTRKPMFSIKPRGLQAGVMFLT
                     SLAIFSVILGFLPLIYIIYAITRNYNGIVINLLLAAGLILPLLVIVQNIAIPILQKIF
                     AKLYPPRPLPHMDFSSGIPDDCKVMCIIPAVIDSPERAKDLVRQLEILYLANREDNIY
                     YSLVGDFKESPRQTEDMDRSIVKAAYDSVEELDKKYGVDNRFFFFYRERVFYEKENKY
                     MGYERKRGAIIEFNNLLIGKSNSFKSNAKGDMPDIKYVLTIDADTFVPGGSIRQMVEI
                     MEHPLNVPVVKDNVVVDGYGILQPSIVQGMSYKSPNFFTSCYSCQPGVDCYFSQTSDF
                     YFDTCYEGIFTGKGMYNPALFNALLEDAFPDNSILSHDLIEGSYLRTAFASNIRFYDS
                     FPGSYDSYVRRLHRWTRGDWQLLPFKKKTIIDKKGRKRKNPLNFLSIFKINANFIRSL
                     LAPSVLLTYIIGMLALRSYFVFWHVLLAITMYLPFILSPSLGLLKQCTLRIVFLPHEA
                     YVMADSIVRTLWRVYVSRKNLLSWVTSADSEKLTKGSFAYYCKNMWFSFIAAIIIPGI
                     TTPIWFFAPFVAKIISNNKCDKVVD"
BASE COUNT         1436 a          800 c         1079 g         1327 t
ORIGIN      
        1 ttattgaagc tgacataaga agaaagaact tccaagagta cgtacccata tacactgagg
       61 aagaagctaa aagagaagct atgcgctgtc ttgaatgcgg ttgcatggat tactttgaat
      121 gcaagttgat cgattatgca aacaagtatg gagttgcacc tgaaagattt gcaggtgatg
      181 ttcataatag aagaaaacca agcacccatc ttcatatagt cagggattcc gacaagtgca
      241 tactctgcgg tctatgtgta agattgtgtg aggaagtaag aggagttacc gctttgggtc
      301 ttgttggacg aggttttgac acaattgtgg agcccgaaat gagaaaagta cttgaggaaa
      361 ccgaatgtga ctcctgcgga ttgtgcgtgt ctgcatgtcc aactggagct cttgtggaaa
      421 aacagtcatt aataaaggca gttcctttga atgaaaaata cacagtatct acatgcgggt
      481 tgtgcagcaa ggcgtgcagc ataaaagttg ctacttatgg aaaccatgtt gtgcgcgtac
      541 tgcctgtatc agatgcgagg actgctgata tactttgcat aaaaggcaga ttcatgtatg
      601 tagatgttct atcaaatgac aggttaaaga gtgcatatat taacgacagg gagtgcgatg
      661 ttgaaaaagc ctgtgagtat ataattgagg aaataagaaa gaatacaggt aaaaaagttg
      721 tttttgtatc tgccgactat acgaacgagc aggtcgacaa agtgcttcta tttgccaagg
      781 caattggtgc ggaagttgtt tccggtatat tgaggcaaca taagtttgat gatgcatctg
      841 cctttgcagg cgtaaacgaa tttggtctga gagtgaaagg cataaaggca ttagaggatg
      901 ctgcaaacct caaggaagaa attgaaaatg gcgatgtgga agtgctggtt tcttttggag
      961 ataaacttga gggtatcaac cttgacaatg ttaatttcaa ggcattcatg agtttgcaca
     1021 tgccaaaaca tgaaggtgtg aatgtattac tgccagacag ctcatatttt gaaatcatcg
     1081 gtacatttac aaaaaagagt gaagacggta cttttgaaga cagggaagta aatgcatgca
     1141 ttaagccgtt tggtggaatt agcagctctg aaatattgga taatttgtgt ggcaaggtgt
     1201 gtgaataatg cgcaacaagg ttatgtatat aacaacggcg gctatgatag ctgccatata
     1261 tacagctctt acattggcag gtgtaggttt tgcttacggg ccagtacagt ttaggttttc
     1321 tgaagcgctg agtattttgc cagtatttac ccctgcggca ataccaggcc ttacaatagg
     1381 ctgtgttata gcaaatctga taggaccgta tggaattgct gacgttgtac tcggctcaat
     1441 cgccacattg ttgtctgcct tgtttgcata tgctacaaga aatatcaggg tttggaaact
     1501 tccgctttta gcgcctttgg gagctgtggt ttttaatgcg tttattgtta gtgcgataat
     1561 attcttcttt acacctgaag atgttgccta ttttatctct gttatctgga tagggttagg
     1621 tcagctggca tcttgctacg gccttggaat acccctcttt tatatacttg acaaatcaaa
     1681 actgtttgac agattcaacg gttatactca ctaaccagaa aaagaattat attagagtat
     1741 ggattaacgg ctaaaagaaa aagccgttaa tttttttata tataaaaaga gaaattggca
     1801 atactaaaag aggaggtgtt tctgtgattt tcaaagaagt acttggtaga gactcattta
     1861 tcaaaagagc ggaaatggct gctcattctc atcatattaa aggaaagtgc aaaaaatggc
     1921 caagacactt gccaaaaccc caagagctaa aagatgtaat tgagaatacg tatagaagga
     1981 ttgcttccag atatatcgaa gaaagaagtg tggcgatgcc tgctgaggaa tggatagcgg
     2041 acaactacca ttttattaaa gggcagctga acacattcga agatctgggc aaacagataa
     2101 agtatcttcc gatgatagaa agaagtgaca ttaaagggta ctcggagctc agaatatact
     2161 caatttgttc cgagatagtc gagtatacgg acggtttggt ggatgaaggt gccatttctg
     2221 attatcttga cagttatcag aagatatctc ctctgaaaac agcggagctt gcagttattg
     2281 cacccatgtt gagacttgcc ttattgaatc gtattgcctt ggtatgcaaa atgaccgata
     2341 gagtaacgga agaaaggcat gagtcagaac ttatgtttaa ccgttacgtt aattacattg
     2401 aaggaaaagg tgcaagcaat agagctcttg aaaaatggat atccagtgtg ggagatttgt
     2461 ctcctgtgtt tgctgagacc attttgagac tcgctgcaaa atggtcggat gacacagaac
     2521 aatttagaaa catcctgaag cgcaagctgg cgggcagggg tatcacactt gatgagctga
     2581 ttgcaaaaga acacaagaca aggagtgcct tgggtgtatc cgttggaaac gcaattacag
     2641 gacttaacaa cttaacatcc atccattgga gtgccttgct aaaaaaactt agcattgtgg
     2701 aacagattct attgaaagac cctgtggatg ttttcagcaa aatgggacga caaagtagaa
     2761 cctactacgt gtacctcata gatgtatatg cgcgaagaat gggtgtttct gcagaaagtg
     2821 ttgcaatgac cgctattgag cttgcctcag cttcatcgtc tccgcagcat gttggatatt
     2881 atctttataa agaagggtat agagacctta agaaggcggt atccgagaaa tttaaaacca
     2941 gaaagccaat gttcagcata aaaccaaggg gcttgcaagc tggtgtgatg tttcttacat
     3001 cattagctat atttagtgtt attcttggat tcctgccgct tatatatatt atttatgcaa
     3061 taaccaggaa ctacaacggc attgtgataa atctgctcct tgcagcaggt ttgattctgc
     3121 cgctgctggt aattgtgcag aatattgcaa tacctatact tcaaaagatt tttgcaaagc
     3181 tttatcctcc aagaccactg cctcatatgg acttctccag cggtatccct gatgattgca
     3241 aggtgatgtg catcataccc gcagtaatag acagtcctga acgggcaaag gatttggtaa
     3301 ggcagcttga gatattgtat cttgccaaca gagaagataa tatctattat tccttggtcg
     3361 gagatttcaa ggaaagtccc cggcaaacag aagacatgga caggagtatt gtaaaagccg
     3421 cttatgacag tgttgaggaa ctggataaga agtatggtgt agacaaccgg ttctttttct
     3481 tttacaggga gcgagttttc tatgaaaaag aaaacaagta catggggtac gaacgaaaaa
     3541 gaggagcaat aattgaattt aacaatctgc ttattgggaa atccaattca tttaaatcta
     3601 atgcaaaggg agacatgcct gatatcaagt atgttttaac gattgatgcg gatacctttg
     3661 tgccaggtgg cagcataaga cagatggtgg aaatcatgga gcaccctctg aacgtgcctg
     3721 ttgtaaaaga caatgttgta gtagacggat atggaatact gcagccatca atagttcaag
     3781 gcatgagtta taaaagcccg aactttttta ccagctgtta ttcatgccaa ccgggtgtgg
     3841 attgttactt ttcacagact tcagacttct actttgacac ttgttatgaa ggaatcttca
     3901 ccggcaaggg aatgtacaat ccagcacttt ttaatgccct gttggaagat gcctttccag
     3961 acaattcaat actaagccac gatttgattg aaggctcata tcttagaacg gcctttgcat
     4021 ctaatatcag attttatgat tctttcccag gcagttacga ttcatatgtg aggagacttc
     4081 atagatggac gaggggagat tggcaactcc tgcctttcaa gaaaaaaacc ataatagaca
     4141 aaaaaggcag gaagagaaaa aatccattga actttctgtc catttttaaa ataaatgcaa
     4201 attttattcg aagtctattg gcgccaagtg tactgctgac gtatataatc gggatgcttg
     4261 cattaaggtc atactttgtc ttttggcatg tgttgcttgc aattacaatg tatcttcctt
     4321 ttatactgtc tccttcgttg gggctgttga agcaatgcac actgcggatt gtatttctgc
     4381 ctcatgaggc atatgtcatg gcagacagta tagtcagaac attatggaga gtgtatgtta
     4441 gcagaaagaa tcttttgtca tgggtgacat ctgcagacag tgagaaactg accaaaggct
     4501 cttttgcata ttactgcaaa aatatgtggt tctcatttat tgcggcaata attataccgg
     4561 gcattacaac tccaatatgg ttctttgccc catttgttgc aaagataata agcaacaaca
     4621 aatgtgacaa agtagttgat ga
//
LOCUS       DAONVY010000137         4617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13398_length_4617_cov_3.182595, whole genome shotgun sequence.
ACCESSION   DAONVY010000137 DAONVY010000000
VERSION     DAONVY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13398_length_4617_cov_3.182595"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLI11_04875"
     CDS             <1..833
                     /locus_tag="PLI11_04875"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPZ52231.1"
                     /translation="FELPSNTAHPSRPVSTPEPYEGSKRYSESPHKRQVDLVETYKNE
                     TSEVYLRKVVIGNGKDRITYYVADVFVSTAMSLKTAFARGKYGMNIKDDIVDIAYDND
                     AVLALSGDYYTNNDIGVVIRNGVYYRSEHTENDICVLYLDGTMKTYSPDEFDIDEVLE
                     RNAWQAWTFGPSLLDGKGNVLKTFNCSSYIKQTHPRAAIGYVRPGHYVLLVVDGREEG
                     YSKGVDIRELAQIMVDEGCIVAYNLDGGKTACMSYDGELVNKPAGGGREIGDIIYIAG
                     "
     gene            856..1071
                     /locus_tag="PLI11_04880"
     CDS             856..1071
                     /locus_tag="PLI11_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52232.1"
                     /translation="MKKLTGKIIFILPNMVVILSLIFITLWILDIFNPGMNFLGNKIS
                     SALLIIFFVLSLINAIATIALERKREE"
     gene            complement(1464..1539)
                     /locus_tag="PLI11_04885"
     tRNA            complement(1464..1539)
                     /locus_tag="PLI11_04885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1504..1506),aa:Pro,seq:tgg)
     gene            1767..2744
                     /locus_tag="PLI11_04890"
     CDS             1767..2744
                     /locus_tag="PLI11_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPZ52233.1"
                     /translation="MKKSQFISLGILLIAVTIFIIVGAFGRGDKDPVVHVTPTPTPSA
                     TTQPLETPTPTNEDVTPTPSPTPTPERTPVVFPTVDKGYVSSQSTETTTWYWGVLKDE
                     NGIEYPTFNQQLNKFVDGFDYVFTNKIDGKKVVTLTFNEGWDDKDNRTVEILNILKEE
                     GVKATFFLTKEYMNVEANHDVIRRMHAEGHQLGTRGSVSRQLQTLSVDDAINELLSME
                     EKLQEILDDNTVRMVAYRHIESFSRRDLALVSELGYKVVLWSYNYIDWGVLNVAEEQK
                     AFNRMIDYMFNGTVFSFSASSVNNVNLLPKLIKEARSQGFEFIQIDTDN"
     gene            2788..3060
                     /locus_tag="PLI11_04895"
     CDS             2788..3060
                     /locus_tag="PLI11_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPZ52234.1"
                     /translation="MTKAVLTVIGIDRVGITARISTCLAEANINILDISQTIMQNIFT
                     MVMLVDLSKSTKNIDEITEELNNIGEELGLNTQLQHVDVFNSMHRI"
     gene            3095..4456
                     /locus_tag="PLI11_04900"
     CDS             3095..4456
                     /locus_tag="PLI11_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HPZ52235.1"
                     /translation="MFISPDEITQTVRMIQEEHLDIRTVTLGINLLDCVTEDIKRTSE
                     KIYDKICRTGSRLVPVCRSIEKEMGIPIINKRIAVTPVSLLVGNVCPEDCVMIARSLE
                     KAAEEIGVNFIGGYTALVHKGYTNGDKSLVKSIPQALNETERVCSSVNVATTKAGINM
                     DAIRDMGHLIKKAAELSKETGGSACARLVVFANAPEDNPFMAGAFHGIGEPECVINVG
                     VSGPGVVKSAIEKVRDKDFTVLAETIKKTAFKITRMGQLIAQEASRNMGVPFGIVDLS
                     LAPTPAIGDSVARILEEMGLECCGAHGTTAALAVLNDAVKKGGTMASSHVGGLSGAFI
                     PVSEDEGMINAVGMGALSLEKLEAMTCVCSVGLDMIVVPGDTPASTISAIIADEVAIG
                     VVNNKTTAVRIIPAPGKKVGDVVDFGGLLGRGPVMKVNPFGSEKFIERGGRIPAPIHS
                     LRN"
BASE COUNT         1535 a          835 c         1076 g         1171 t
ORIGIN      
        1 cctttgagct accttcgaat acggcacatc cgtcaaggcc tgtatcgact ccagaaccct
       61 acgagggatc caagagatat tctgaaagtc cacacaaaag acaagtggat ttggttgaaa
      121 catataagaa tgaaacaagt gaagtctatt tgaggaaagt ggtaatagga aacggtaaag
      181 acaggattac atactatgtc gctgatgtat ttgtcagcac tgctatgtct ttgaaaactg
      241 cctttgcaag aggcaagtat ggcatgaaca ttaaagacga catagtggat attgcatatg
      301 acaacgacgc cgtacttgcc ctttcaggag actattatac caacaacgac ataggggtag
      361 ttatcagaaa cggagtgtat tacagaagcg agcatacgga aaatgatata tgtgttctgt
      421 atctcgatgg aactatgaaa acctactccc cggatgagtt tgacatagac gaggttttgg
      481 agaggaatgc atggcaggcc tggactttcg gccccagtct tctggatggc aagggcaatg
      541 ttttgaaaac ctttaactgc tcatcataca taaagcaaac tcacccgaga gcagctatcg
      601 gctatgtaag accgggtcat tacgtactgc tggttgtaga cggaagggaa gaaggctact
      661 cgaagggcgt agacataaga gagcttgcac aaatcatggt ggacgaagga tgcattgtag
      721 cctacaatct tgacggaggc aagactgcat gcatgtctta tgatggagag cttgtaaaca
      781 agcctgcagg tggcgggcgg gaaataggtg acataattta catagctgga tgacgacaag
      841 agagaagggg attttgtgaa gaagttaaca ggaaagataa tttttatact gccaaatatg
      901 gttgtaatac tctcgttgat attcattacg ctctggattc tagatatttt caatccgggg
      961 atgaattttc ttggaaataa aatatctagc gctctgctta tcatattttt tgtactatca
     1021 ctgattaatg ccattgcaac cattgctctg gaacgaaaaa gagaggagta aaaagtaaaa
     1081 gatccgaata tcaatgatac tcgtgtcata ttatgtgttc tactgataaa aaagatgcag
     1141 acggttactt tgcgttcaat gttagcttac ctattattgt ttgaaacctc ttttcatagc
     1201 ggatcggatc gtaacgtttg tccttcaatc ggtccagcat ataccaacat agtgtgtgct
     1261 catcgagctc atgaaattct ttgctagacc ccctatatac aagatattct tttctctttc
     1321 ggactgatta taccctagta attcatctac agtttcaatt cattgttgta gtataaatga
     1381 atcgtttacc ttcaaaccta cacaaaataa tatgaagaaa aaaagcaaca acaatcctct
     1441 tgtagaaaat tgtcgttgct ttttggtcga ggtgacaggg attgaacctg cggcctcatg
     1501 gtcccaaacc acgcgcgctc ccagctgcgc cacacctcga tacgtcattt gcgacgcttg
     1561 aatatattaa cacacaaaaa ttgaattgtc aacacctgta tttataatat tttttcccag
     1621 aatgcaactg gaataaatct aatttgtcaa gaatacgtag aaaaaaaact acaatatttc
     1681 cactagaaat attgccgttt gtccattgat gtgataatat atatgtggtt aaaatctact
     1741 aaattacgac tggagcatga aatataatga aaaagagtca gttcatatca ctaggcatat
     1801 tgttgattgc tgttacgata tttataatag ttggggcatt tggaagggga gacaaggacc
     1861 cggttgttca cgttactccg actccaacac caagtgctac aacacagccc ctcgaaacgc
     1921 caacacccac aaacgaggat gtaacaccca cgccttctcc tacgcctaca ccggagagga
     1981 cgcctgttgt gttcccgaca gtagacaagg gatatgtaag ctcacaatca actgaaacca
     2041 caacctggta ttggggggta ttgaaagacg agaacggtat tgagtatccg acatttaacc
     2101 agcaattgaa caagttcgta gatggatttg attatgtgtt tacgaataaa atagatggca
     2161 aaaaggttgt aacccttaca ttcaatgagg gttgggatga caaggacaac agaaccgttg
     2221 aaatactcaa catattgaaa gaagagggcg taaaggcgac atttttccta acaaaggaat
     2281 acatgaatgt agaggctaat catgacgtta taagacgaat gcatgccgaa ggacaccagc
     2341 tgggtaccag gggttccgta tcaagacagc tgcaaactct ttccgtagat gatgcaataa
     2401 atgagcttct gagtatggaa gagaaattgc aggaaatcct cgatgacaac acggtaagaa
     2461 tggtggcata caggcatatc gagagtttca gcagaagaga cctggcactg gtatccgaat
     2521 tggggtacaa ggttgtactc tggtcctaca actatattga ttggggagta ctcaatgtag
     2581 cagaggaaca gaaagcattc aatagaatga ttgattacat gttcaacggg accgttttct
     2641 cgttttcagc atcaagcgtt aataatgtga atctattgcc taaattaata aaagaggcga
     2701 gaagtcaagg tttcgaattt attcagattg atacagataa ctaaccaaga aacggcccgt
     2761 cgggtcgttt tttaaaagga gcgtattatg acaaaagcag tattgacggt aataggaata
     2821 gatagagttg gaataacagc aaggataagt acatgcttgg ctgaagcaaa tataaacata
     2881 ttggatataa gccagactat catgcaaaat atatttacca tggtaatgct ggtggatttg
     2941 agcaagagta ctaaaaatat tgatgagatc actgaagagc tgaacaatat cggcgaggaa
     3001 cttggcttga acacacagct acagcatgta gatgtattta actcaatgca caggatttga
     3061 atatactaaa atagactgag aataggagat tgacatgttc atatcaccgg atgaaataac
     3121 acaaacagtc agaatgatac aggaagagca tctggatatc agaactgtca cgttaggcat
     3181 aaatttgctg gattgtgtga cagaagacat aaagagaaca agtgaaaaaa tatatgacaa
     3241 aatatgcaga acaggatcca gacttgtgcc tgtctgcaga agtatagaaa aggaaatggg
     3301 catccccata attaacaaaa gaattgcagt aactcccgta tcgctattgg tcggaaacgt
     3361 atgtcccgaa gactgcgtaa tgatagcaag aagccttgaa aaggcggcgg aagaaattgg
     3421 cgtaaatttt atcggtggat acactgccct tgtccataaa ggctatacca acggagataa
     3481 atcccttgtc aaatccatac cacaagcact gaatgaaacc gaaagggttt gttcaagcgt
     3541 taatgtggcg acaacaaaag ccggcattaa catggatgcg ataagagaca tgggacatct
     3601 tataaaaaaa gcagcggagc tctctaaaga aacaggtgga agcgcatgtg caaggcttgt
     3661 tgtctttgcc aatgcacccg aagataatcc gttcatggct ggcgccttcc acggaatagg
     3721 agagcctgag tgtgttatca atgtaggtgt aagtggaccg ggagtagtta aaagcgcaat
     3781 agaaaaggtt cgggacaagg attttacagt acttgcagaa acaataaaga aaacagcatt
     3841 caagataaca agaatgggac agcttattgc ccaggaagcc agcaggaata tgggtgtgcc
     3901 ttttggaatt gtggatttgt ctcttgcacc cacaccagcc ataggcgaca gcgtggcaag
     3961 gatactagag gaaatgggac tggaatgctg tggagcacat ggaaccaccg ctgctctcgc
     4021 agtattgaat gatgcagtca agaaaggcgg aacaatggca tcttcccatg tgggagggct
     4081 aagcggtgcc ttcataccag taagcgagga cgagggtatg ataaacgcag taggcatggg
     4141 agcattgtcg ctggagaaac tggaagcaat gacctgcgta tgctccgtcg gacttgatat
     4201 gatagtagtt ccaggcgaca cgccggcatc aacgatatcc gcaatcatag cagatgaagt
     4261 agctattgga gtagttaaca ataagacgac tgcggtcaga ataataccgg cacctggaaa
     4321 aaaggttggt gatgttgtgg attttggagg tcttttaggc agaggacctg taatgaaagt
     4381 aaacccattt ggaagtgaga agtttataga aagaggagga agaattccag cacccataca
     4441 cagccttaga aactgacgtg ttaatataat taatcctgtt gcatatatat aataagagcc
     4501 ttgcaaaagt taataaactt ttatgcttga tattttttta tggcaatgat ataattaaat
     4561 tgttaataag gagaattgga tattggccaa aagacaggat tggagtgaag caaatga
//
LOCUS       DAONVY010000138         4578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13544_length_4578_cov_3.964846, whole genome shotgun sequence.
ACCESSION   DAONVY010000138 DAONVY010000000
VERSION     DAONVY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13544_length_4578_cov_3.964846"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLI11_04905"
     CDS             complement(<1..938)
                     /locus_tag="PLI11_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52236.1"
                     /translation="MNAYRDIMSRLGPFRVKEGFEHKKTVASIEALANRADLISRGAR
                     PDTYFGQPPDGISYDNLITRAYRHLESLERGELPLKGKFCEPGGSCSDHSVVVVDDKV
                     HLFYTIDSIGYDWPERYVHYIGHASSTNLVDWEIHKPAVAIHPEGHEVYQVWAPAVIH
                     HDGMYWMFYTGVNYNVSQATVLATSKDLYNWERYEKNPLYYPTRWNKWTQESWFDNRD
                     VMIFKDNDTFYMYFYTAGLREDGSAFPACGVASSKNLVDWKDETLIELGCKYACESPF
                     MVKHENKYYLFYTDCGKGTSYAVSDNPLGGFEVKGLL"
     gene            complement(954..2321)
                     /locus_tag="PLI11_04910"
     CDS             complement(954..2321)
                     /locus_tag="PLI11_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPZ52237.1"
                     /translation="MDAISEIVNKQREFFNSGRTKDVKFRIEMLNKLKQAVLNYEDRI
                     MDALFTDLNKDAFDSYTTEIGFVLNEISYAARKLPKWAKRKRVRTDIASFPAKSFIYP
                     EPYGVTLIISPWNYPFQLAIAPLVGSIAAGNCAVIKPSSYSPNTSKVLKQLIEDTFDE
                     EYVCVIEGGREANKALLDSKFDYIFFTGSVSVGKVVMESAAKHLTPVTLELGGKSPCI
                     VDETANLDLAAKRIIWGKLINVGQTCVAPDYLLVHSSVKNELVEKMKRVVEDFYGSNP
                     VENEGYTKIINEKHFNRLLGLVKGEKVIYGGEFDEKRLKINPCFIDNASWDSPVMQEE
                     IFGPVLPIIEFENLEEVIERIKQRPKPLALYYFTTSKDREEKVLNSVSFGGGCINDTI
                     MHLVSRHMPFGGVGESGMGSYHGRKSFDTFTHYKSILRKSNVIDVPLRYPNRKNLKLV
                     KKILK"
     gene            complement(2334..2693)
                     /locus_tag="PLI11_04915"
     CDS             complement(2334..2693)
                     /locus_tag="PLI11_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HPZ52238.1"
                     /translation="MGRNSNEKIYSMSFAKVYPLYVSKAERKGRTKQEVDEIILWLTG
                     YTQEELLGHLEKESTFEEFFSQAPELNPNRMLIKGVVCGVRVEEIEEPLMREIRYLDK
                     LVDELAKGKAMEKILRK"
     gene            complement(2790..3650)
                     /locus_tag="PLI11_04920"
     CDS             complement(2790..3650)
                     /locus_tag="PLI11_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPZ52239.1"
                     /translation="MSKQLKADISLLVISMIWGAGMPVMSLALQHIGPYTFMLTRYSI
                     ATIILLPFVVKRLRKTEKESIKGGALIGLSMFLGSLLQTIALLYTTPSKAGFLTGLYS
                     IFVPVITAVIYRKLPDLKTVFAVGISLFGLAVMSIEGSSGINIGDVLLVVSAVAYAFQ
                     ILLVGKYAKSEYILETTFMQMLVVSLLSFIPSMAMEKFYVPINKTTVLSVLFIAVFST
                     VVAYMVQNKMQPYTDPTHAAIIFLSEPLFVAIFSIFVGDILTVRTLIGGVFIMLGMLV
                     INIKTGKNEG"
     gene            complement(3666..>4578)
                     /locus_tag="PLI11_04925"
     CDS             complement(3666..>4578)
                     /locus_tag="PLI11_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52240.1"
                     /translation="SSAINFARETTELSKNIVMMGQTLRFLDPFMEFMSLPDEKDVGG
                     DVESEGEVESIEFRNVSFKYPGSDMLVLDDISFKISKGEKISIVGLNGAGKTTLVKLV
                     CRLYRPMSGKILINGRDVFEYDYTSYMKKIAAVFQDYKLFAFSIEENITCKDVGEDTE
                     RAMAILEEVGMTEKVQSLPEGIRSRIGKAYDEKGIEMSGGQTQKIAIARALYKDASLV
                     ILDEPTSALDPIAEADIYANFNKLIGKKTAIYISHRMSSSVFCDRVLIIEKGKVQDYD
                     THENLLKKTDSLYYKLFQSQAVNYKID"
BASE COUNT         1254 a         1115 c          804 g         1405 t
ORIGIN      
        1 agaagacctt tgacttcaaa accaccaagc ggattgtctg atactgcata ggaagtacct
       61 tttccgcagt cggtatagaa aagataatat ttgttttcat gctttaccat gaaaggagat
      121 tcgcaggcat atttgcatcc aagttcaatc aaggtttcat ccttccagtc aacaaggttc
      181 tttgacgacg caacaccgca tgcaggaaaa gcacttccat cttctctaag gcctgcggta
      241 taaaaataca tgtagaaggt atcattatcc ttaaatatca tcacatcgcg attgtcaaac
      301 cagctctcct gtgtccactt gttccatctt gtcggataat acaagggatt cttctcatac
      361 ctttcccagt tgtacaaatc ctttgatgtg gcaagaacag ttgcctggga aacattgtag
      421 ttaacccctg tatagaacat ccagtacatg ccgtcatgat gaatgaccgc aggagcccat
      481 acctgataaa cttcatgtcc ttcaggatgt attgccactg caggcttgtg tatttcccaa
      541 tccacaagat tcgtacttga cgcatgtcct atgtagtgta catatctttc cggccagtca
      601 taacctatgg aatcaatggt gtaaaaaaga tgaaccttat catcaactac aaccactgag
      661 tggtctgaac aggagcctcc cggttcacaa aacttgcctt taagaggcag ctcccctctt
      721 tcaaggcttt ccagatgcct gtacgctctt gtaataagat tgtcatacga gattccatcc
      781 gggggctgcc cgaagtatgt atcggggcgg gcgcctcttg aaatcaaatc cgccctgttt
      841 gcaagtgctt caatgctggc cacagtcttt ttgtgctcaa aaccttcctt gactctgaaa
      901 ggtccaagtc tgctcataat atccctgtat gcgttcataa aggcctccca tatctatttt
      961 aatatctttt taaccagttt taaattcttg cggtttggat atcttagtgg tacatctatc
     1021 acatttgatt ttcttaaaat gctcttgtag tgggtaaagg tgtcaaagct tttcctgccg
     1081 tgatagctgc ccatgccgct ttcaccaaca ccaccaaaag gcatatgcct ggatacaaga
     1141 tgcatgatgg tgtcgtttat gcatccgcct ccaaaggaaa ctgagtttaa gaccttctct
     1201 tccctatcct tgcttgttgt aaagtagtaa agggcaagag gttttggcct ctgttttatt
     1261 ctctcaatta cctcctccaa attctcaaac tcgataatcg gaagaacagg accaaagatt
     1321 tcttcctgca tcacgggact gtcccaggat gcattatcaa tgaaacacgg atttatcttc
     1381 aatctctttt cgtcaaattc tcccccgtaa attaccttct caccttttac aagtccgagc
     1441 aatctgttaa agtgcttttc atttatgatc ttcgtgtagc cctcattctc aacagggttc
     1501 gaaccataaa agtcttccac aacccttttc attttttcaa ccagttcgtt ttttacactt
     1561 gaatgaacca acagatagtc aggagcaacg caggtctgac ccacatttat gagtttgccc
     1621 cagataattc tttttgcagc caaatccaga tttgccgttt cgtcaactat gcagggactt
     1681 tttccgccaa gctccaaggt taccggtgta agatgtttgg ctgcactttc cataaccact
     1741 tttccaacgg aaacacttcc tgtaaagaat atatagtcga acttgctgtc aagcagtgcc
     1801 ttgttggcct cccttccccc ttcaattaca catacgtatt cctcatcaaa ggtgtcttca
     1861 atcagctgct tgagtacctt tgatgtgttt ggagaatagg acgacggctt tataaccgca
     1921 caatttcctg ccgcaatact tcccacaaga ggagcaattg ccagctggaa aggatagttc
     1981 catggtgata tgatcaaggt tacgccatag ggttcaggat agataaagct ttttgctggg
     2041 aaagatgcaa tatccgttct gactcttttt ctttttgccc atttgggaag ctttcttgcc
     2101 gcataactta tctcattcaa aacaaaacct atttcagtag tgtatgagtc aaaggcatcc
     2161 ttgttgagat ccgtaaaaag cgcatccatt atcctgtctt cataattcaa gactgcttgt
     2221 ttcagcttat taagcatctc aatccggaac tttacatcct ttgtccttcc actattgaaa
     2281 aactcccttt gtttattgac aatctcactg atggcgtcca tgttcaccac cctttacttt
     2341 cttaatatct tttccatcgc ctttcctttg gccagttcat caaccagctt gtccaaatag
     2401 cgtatttccc tcatcaaagg ttcttcaatt tcctccaccc ttacaccgca taccacacct
     2461 tttatgagca tgcggtttgg gttaagctct ggtgcctgag agaaaaattc ttcaaaggta
     2521 gattcctttt caagatgtcc taaaagctct tcctgggtgt atcctgtaag ccacaagata
     2581 atctcgtcaa cttcctgttt ggtacgccct ttcctttcag ccttgcttac ataaagagga
     2641 taaaccttgg caaaactcat ggaatatatt ttttcattac tgtttcttcc cataacaatc
     2701 cttcctggtg tttttcttta tgctaccata atttatttcg aaatacaaaa aaacaacaaa
     2761 aacgtgtaac acggttgttt tccaaccttt tacccttcat tcttcccagt ctttatgttg
     2821 ataaccagca tccccaacat tataaacacg ccgccgatga gggttctgac agtcagtata
     2881 tctcctacaa atatggagaa aatggctaca aaaagtggtt ctgacaggaa tataatggct
     2941 gcatgggtag ggtctgtgta gggctgcatc ttgttctgca ccatgtatgc aaccacagta
     3001 ctgaataccg ctataaacaa aactgaaaga accgtggttt tgtttatggg aacgtaaaac
     3061 ttctccattg ccatggaagg tatgaaagac aaaagactta ccaccagcat ttgcataaat
     3121 gtggtctcaa gtatgtattc actttttgca tacttgccca ccagcaaaat ctgaaaggca
     3181 taagctactg cactgacaac caaaagaacg tcaccaatat tgattccaga agatccttca
     3241 atcgacatta cagcaagacc aaacaaactt attcccactg caaaaacagt ctttaaatcc
     3301 ggcagctttc tgtatattac agcagttatt acaggaacaa atattgaata caaacctgta
     3361 agaaaaccgg ccttgcttgg ggttgtatat aatagggcta tggtttgcag aagacttccc
     3421 agaaacatgg acaaacctat aagagctcca ccttttatac tttccttctc tgtttttctc
     3481 aatcttttaa ccacaaaagg aagtaatata attgtggcaa tggagtatct tgtcagcatg
     3541 aaagtataag ggccaatatg ttggagagca aggctcatta ccggcatgcc cgctccccaa
     3601 atcatactaa taaccaacaa agaaatatcc gcttttagtt gtttactcat tcaatcctct
     3661 ttctatcaat ctattttgta attcacagcc tgtgactgga acaacttgta gtacaaacta
     3721 tccgtcttct tgagcagatt ctcatgggtg tcgtaatcct gcacctttcc cttctcaata
     3781 atcagcactc ggtcgcagaa tacgctgctg gacattctgt gggatatgta tatggcagtt
     3841 ttcttaccaa tcagtttgtt gaagtttgca tatatatctg cttcagcaat cggatcaaga
     3901 gctgatgtcg gctcatcaag aatcaccagc gaagcatcct tgtacaaagc tcttgctatt
     3961 gcaatcttct gggtctgacc gcctgacatc tcgatacctt tttcatcgta tgcctttcca
     4021 attctcgaac ggatgccctc tggaagactc tgcacctttt ccgtcatgcc cacttcttcc
     4081 agaatagcca tggctctttc ggtatcttct ccaacatcct tgcaggtaat gttctcctca
     4141 atactgaatg caaacagttt gtaatcctga aaaaccgcag caatcttctt catgtagctt
     4201 gtgtagtcat actcaaacac atccctgccg tttattaata tcttgcctga cataggtctg
     4261 tacagcctgc aaacaagctt taccaaggta gtctttccag caccgttcaa tcctacaatg
     4321 gatatcttct caccctttga tatcttgaag gatatgtcat caaggacaag catgtcgctt
     4381 ccaggatact tgaaggatac atttctaaac tcaatggatt caacctctcc ttcagactcc
     4441 acatctccgc ccacatcctt ttcatcaggc agagacatga attccatgaa aggatccaga
     4501 aatctcaatg tctgacccat catgacgatg tttttggaaa gctcggttgt ctcccttgca
     4561 aagtttatgg cactagac
//
LOCUS       DAONVY010000139         4567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13588_length_4567_cov_3.502216, whole genome shotgun sequence.
ACCESSION   DAONVY010000139 DAONVY010000000
VERSION     DAONVY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13588_length_4567_cov_3.502216"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..789
                     /locus_tag="PLI11_04930"
     CDS             439..789
                     /locus_tag="PLI11_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HPZ52241.1"
                     /translation="MGYKEKVKHFYVIRIGNDVFPAGVEGMKQHLIEVRKTYPDLKMT
                     IIRQYIDGDTVISEFVMEGTHLGEWLGMKPTGKKLRMTGVDIDRLMDGKIVEYGGAVN
                     TFEALSGEGIIKPA"
     gene            815..1291
                     /locus_tag="PLI11_04935"
     CDS             815..1291
                     /locus_tag="PLI11_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ52242.1"
                     /translation="MEVDISIVEVKDSKKKGQFAEEVMRYLPEWFGNEKALVEFVDGV
                     TSLPFWAALDHEDKCIGFISIKIHYGHTGDIYVFGVLPEYQGRGIGRLLLAKAEEYLK
                     NNNCKYIIVKTLSDIVEYEPYERTRLFYKKVGFEELITLTEMWDSENPCLIMIKEL"
     gene            1358..2281
                     /locus_tag="PLI11_04940"
     CDS             1358..2281
                     /locus_tag="PLI11_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52243.1"
                     /translation="MFNVIDQIQELSPCAAVRYRIRRILKQEIDSELFTEFYNSKWVE
                     LLKANQLDDGGYGRFHSRDSKTKQKFSTTETALNSMKMLDLRRGNSLVDKSCDYMEKI
                     LRNEIDWPDGFEKNRWYRSAQPLFVATKLSIFGSNCMEFKDIFNCWHAILKEAFENGE
                     YNKDRTNKISKDLLGCDIDGSYIGLNSIYLIELFGNMQAEISDELKLNYLRWLHHNGQ
                     VVRYTNVVLNHGFDNNFSELYKVYLLLSKFPCFKKEFEEELRCLKDKRNKDGFWNFGK
                     NFKCQKLSDDWRRQENMKIDHTVLALLLYID"
     gene            2313..2777
                     /locus_tag="PLI11_04945"
     CDS             2313..2777
                     /locus_tag="PLI11_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPZ52244.1"
                     /translation="MKTAILYESWHHGNTKRLCDEIKKEYQDVILLNVKEDVIALEEY
                     DLIGIASGVAFGGFYKEILQVTKDKMPEGKNVFLMYTCGKTGPDYAKSIKRILSKKNC
                     NVLGTYYCKGYDTYGPFKLVGGISKGHPTESEIQGAVRFYGEIISKYINFKE"
     gene            3131..3751
                     /locus_tag="PLI11_04950"
     CDS             3131..3751
                     /locus_tag="PLI11_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPZ52245.1"
                     /translation="MYKIIEYLKKEYNPLAIVVYGSYASGTNDEYSDFDCMIIVEEKS
                     RKHDDSVINGIQLDCFIYTKKEIQSKNIDEFIAVFNGDIVLDTEYIAENLKTRVQEYV
                     KDHSITSMEEKQFIISWIKKTMRRAEKNDDEGNYRAVAFLSESLADYFQLRDLFYFGS
                     KRSISYLRDYDKAGYQLYHKAITERSNAAISLWAEHVINIDLKRCS"
     gene            3818..4381
                     /locus_tag="PLI11_04955"
     CDS             3818..4381
                     /locus_tag="PLI11_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPZ52246.1"
                     /translation="MRSFTTLELQYAHRFYGFKGEAQYLHGHTGILTIEVEDTVNMGV
                     NMVYPCNEINKIAWEVLQNFDHALVLREDDPLLPAILSVYEAQGIKDGAPNNMQKGPA
                     FKTELATAYPECRLEVTKETMTVEGMIKIVYDLLKDKLNIVKITFTSGVNGATEEYEP
                     NKKLDRCPLCGVTLNENGVCPKCGYKK"
BASE COUNT         1560 a          638 c         1030 g         1339 t
ORIGIN      
        1 cagatggaca tctgaggctc gcaagatata tggggacaga tactgaaaac cttatagttt
       61 gaaaatatgt ttagacagag ctgttggcgg gctataaggc ttgattttac tggatttgct
      121 ggactatatc caagttgtct attctcacag aggtatccga cggaaagaag aagttacgca
      181 gctgttttca tgctcttatg ttcattgttt catatctaac aagtttctaa aaatcgtttt
      241 aggctatcag gttaatcaat aatcattcat gaaagttcgt gattatcttt ttgggtgtga
      301 ttaatccttg atgaatgttt ggaaaaccaa aaatgaacca ttcatttggt actcggattt
      361 ttcgatgttg agtaatcagg ttaatactgg tagaatctat tatctagtaa gcaaataaat
      421 cattgcagga acaggaatat gggttataag gaaaaagtca agcatttcta tgttattaga
      481 ataggtaatg acgtttttcc ggcaggcgta gagggaatga aacaacatct aattgaggtt
      541 aggaaaactt acccggactt gaagatgaca attatccgcc aatatatcga tggcgataca
      601 gtaatctcgg agtttgtcat ggagggtacg catctgggtg aatggctggg catgaagccc
      661 acgggaaaga agctgcgaat gacaggtgtt gatattgata ggcttatgga tgggaagatt
      721 gtcgagtatg gtggagcagt aaatacattc gaagctcttt ctggagaagg gattattaag
      781 cctgcataat gatgatttaa aaacagggag gaatgtggaa gtggatataa gtatagttga
      841 agtgaaggat tcaaaaaaga aagggcagtt tgcagaagag gtcatgagat atttgccaga
      901 atggtttgga aatgagaaag cacttgttga gtttgtagat ggtgtgacat ctctgccatt
      961 ttgggctgcc ttggatcatg aagacaaatg cattggtttt atttctataa agattcatta
     1021 tggtcatacg ggcgatatct atgtttttgg agtattgcct gaatatcagg gtagaggcat
     1081 aggaagatta ttgttggcta aagcagaaga gtatctaaag aacaataatt gcaagtacat
     1141 aattgtaaaa acgctaagcg atattgtgga atatgaaccc tacgaaagaa caaggctgtt
     1201 ttataaaaaa gttggtttcg aagaattaat aacattaact gaaatgtggg attctgaaaa
     1261 tccttgcttg attatgatta aagaattgta agtacaatgc atgcatggta aatgctgttg
     1321 gtggttgttt tgaccaggaa aggaagtagg cttaagcatg ttcaacgtaa ttgaccaaat
     1381 tcaggaatta tctccttgtg ctgcggttag atacagaatt agaagaatac tcaagcaaga
     1441 aattgactct gaactgttta ctgaattcta taatagcaag tgggttgaat tattgaaagc
     1501 aaaccagctc gatgatggtg gatatggaag gtttcattcc cgcgactcaa aaacaaagca
     1561 gaaattttca acgacagaaa cagcacttaa ttctatgaaa atgctggatc ttcgacgtgg
     1621 caattcgttg gttgataaat catgtgacta tatggagaaa atattaagga atgaaattga
     1681 ttggccagat ggatttgaaa aaaatcgatg gtacagatct gctcaacctt tgtttgttgc
     1741 aaccaaactc tcaatatttg gttcaaattg catggagttt aaggacattt tcaattgctg
     1801 gcatgctatt ttaaaagaag catttgagaa tggtgagtat aacaaggata gaacaaacaa
     1861 gatatcaaaa gatttattgg gttgtgatat tgatggaagt tatattgggc tcaactcaat
     1921 atatcttatt gagttgttcg gcaacatgca agctgaaata agtgatgagt tgaaactaaa
     1981 ttaccttaga tggctgcatc ataatggaca ggttgttcgc tatacaaatg ttgtactaaa
     2041 tcatggcttt gataacaatt tttctgagtt gtacaaggtg tatttacttc tttccaaatt
     2101 tccctgcttc aaaaaagaat ttgaagaaga actacgctgt ctgaaagaca aaaggaataa
     2161 agatggtttt tggaactttg gcaagaattt taaatgccag aaactatccg atgactggag
     2221 acgacaggaa aacatgaaaa ttgatcatac tgttttggct ttgcttttat acattgatta
     2281 aacttttgaa acaagggata gggggaataa agatgaaaac agcaatatta tatgaatcct
     2341 ggcaccatgg aaatacaaaa agactttgtg atgaaataaa gaaagaatat caagatgtca
     2401 tacttctcaa tgtaaaagaa gatgttattg ctctggaaga atatgatctt attggtattg
     2461 catccggtgt tgcctttggc ggattttaca aagaaatttt gcaagtaaca aaagataaaa
     2521 tgcctgaagg caaaaatgtt tttcttatgt atacctgcgg aaaaacaggg ccggattatg
     2581 caaaaagcat aaagagaatt ctatctaaga aaaattgtaa tgttcttggc acttattact
     2641 gcaagggata tgatacatat ggacctttca agcttgttgg tggaattagc aaggggcatc
     2701 ctactgaaag tgaaattcaa ggtgctgtga ggttctatgg tgagataatc agtaaataca
     2761 tcaatttcaa agaataaaag gggaaaaaca aggaattgaa aacgcctttt acgcaaggaa
     2821 gtgagcatcc atgcagttgg agttttatcc tgccaatctt attaattggt ctcatatttt
     2881 cccgaataga ccatcctggt gaaaatatgt aattgttgtt taatgggttg gctgccttgg
     2941 aataatcatt ataccattgt tctgggaaat aagatacgca gaaatggaga ttaaacatga
     3001 gtactatcag gattctactt aaaatcataa tgtggtactt aattgcaggg ttttgatgca
     3061 acttaaacaa ttgctattca tttttaaatg aaaaatatat agagataaaa tatattagga
     3121 gttagagtaa atgtataaga ttattgaata tttaaaaaaa gaatataacc cgctggcaat
     3181 agttgtatat gggtcctatg cgtccggcac aaatgacgaa tacagtgatt tcgactgtat
     3241 gatcatagta gaggaaaaaa gcagaaagca tgatgattct gttatcaacg gcatccagct
     3301 tgactgcttt atatatacca aaaaggaaat ccagagcaag aatattgacg aatttatcgc
     3361 agtttttaac ggggatattg ttttggatac agaatacatt gctgaaaact taaagacgag
     3421 agttcaggag tatgtgaaag accattccat tacttcaatg gaagaaaaac agtttatcat
     3481 ttcctggata aaaaagacga tgcgtagagc agaaaagaac gatgatgaag gaaactacag
     3541 ggcagttgca tttctatctg aaagccttgc cgattatttt cagctgcgag atttgtttta
     3601 cttcggaagc aaaagatcca tttcgtattt gagagattac gataaggctg gataccagct
     3661 ttaccataaa gcaatcacag aaagatcaaa tgcagcaatt tcattgtggg cagaacatgt
     3721 catcaatatt gatttaaaaa ggtgtagtta gaaaaaattt tccagggtgt ttgtatatta
     3781 ctattagaaa tggtacattt agcttagcat taattgtatg agaagtttca caacattaga
     3841 actgcaatat gcacacagat tctatgggtt caagggcgag gcacagtatt tgcacggaca
     3901 tacgggaata ctgacgattg aggttgaaga tactgtaaac atgggtgtca acatggtgta
     3961 tccatgcaat gaaatcaata aaattgcttg ggaagtctta cagaacttcg accatgcgtt
     4021 ggttttaaga gaagatgatc cgttgctccc tgcgattctc agtgtttatg aagcacaagg
     4081 tattaaggat ggtgcaccaa acaacatgca aaaaggacct gcctttaaga cggagcttgc
     4141 tactgcatat ccggagtgtc gtttggaagt cacaaaagaa acgatgacag tagaagggat
     4201 gattaaaatc gtatatgacc ttcttaagga caagctgaac atcgtgaaga ttacatttac
     4261 gagtggagta aatggggcta cagaagagta cgagcctaat aagaaattag atcgctgtcc
     4321 gttgtgcgga gtcacattga acgagaacgg tgtatgccca aaatgtggat acaagaaata
     4381 ggctgaaatg atgatttgac ctttacaagt cactctatgc aatatttgtg tagagtgact
     4441 tgtttgtttt caggcactaa gaagtggcaa ctcatatcag agaagcattg ggtgaaatcg
     4501 gaggttagat aatgagcaaa ttgattatca ggcgtgaaac accagttgat taacaaagag
     4561 gctggtt
//
LOCUS       DAONVY010000140         4533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13692_length_4533_cov_4.033274, whole genome shotgun sequence.
ACCESSION   DAONVY010000140 DAONVY010000000
VERSION     DAONVY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13692_length_4533_cov_4.033274"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLI11_04960"
     CDS             complement(<1..558)
                     /locus_tag="PLI11_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPZ52247.1"
                     /translation="MARRKEVDYFGLFKKAIDYSCVLTKELTTLVEEYKDINKNGQLE
                     KRVDEIHKIEHDADKVYHEIVFELNRAFITPIEREDILLIANAIDDVSDKIEEVAFSF
                     WVFNITEMRPETKQFIDLVNRICKESTEAVYEFSDYRKSKTIKDKLHTIKKLENEGDT
                     LYRHSVRRLFENEKDPIALQKWREIY"
     gene            complement(605..1690)
                     /locus_tag="PLI11_04965"
     CDS             complement(605..1690)
                     /locus_tag="PLI11_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPZ52248.1"
                     /translation="MFLADATVPSLLEFISLCTKNVPLLITSLLVIGVIFVNGWTDAP
                     NAIATVISTRSMGPRKSIIMAAIFNFLGVLFMTFWNASVAMTMYNLVDFGKDTETALV
                     ALCAAFVAIVVWAVGAWYFGIPTSESHALIAGVTGAAVAINGFKGAINNDEWLKVIKG
                     LFFSTVLGFFVGFIFVKIIIFFFKGTNRRKSNRFFKGGQIAGGAAMAFMHGAQDGQKF
                     LGIFMLGLMLAQGVPVTASTEFDLSGALWLIIMCSLIMGLGTSIGGYRIIKSVGMDMV
                     KLEKHEGFSADFAAAFCLLLSHFYGLPVSTTHTKTTAIMGVGAAKRLTNVNWSVAKEM
                     FAAWFLTFPGCGLIGYVMAQIFLKLFA"
     gene            complement(1764..2291)
                     /locus_tag="PLI11_04970"
     CDS             complement(1764..2291)
                     /locus_tag="PLI11_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPZ52249.1"
                     /translation="MQQIIISGFGGQGILSMGRLLAAGGLHEGHNVSWLPSYGPEMRG
                     GTANCHVIISNEEIGSPIISCPNALIVMNQPSLDKFENMVEENGIIVMDSALVPIKPK
                     RQDLRSFSLPATEKAYEMGHPAFAGVILLGKFIAETGIISSKSFEEALRTVLSPSKHN
                     LIPDEMKALEFGMSY"
     gene            complement(2293..3039)
                     /locus_tag="PLI11_04975"
     CDS             complement(2293..3039)
                     /locus_tag="PLI11_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPZ52250.1"
                     /translation="MAIVFKKPNALTDKPFHYCPGCTHGIIHRLVAEVIDELDIEGET
                     VGVSPVGCTYNNYEYFSCDMVQAAHGRAPAVATGLKRALPDKVVFTYQGDGDLAAIGT
                     AEIVHAATRGEKITTIFVNNAIYGMTSGQMAPTSLIGQVTTTSPFGRKPQLHGYPVKV
                     SEMLATLEGAVYIERVSVDSIKNIIKAKKAIKKAFQVQLEGLGFSMVEVLSTCPTNWG
                     MSPAKSLDWLRDNMAEHYPLGVYKGKDLEV"
     gene            complement(3050..4111)
                     /locus_tag="PLI11_04980"
     CDS             complement(3050..4111)
                     /locus_tag="PLI11_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPZ52251.1"
                     /translation="MGKRILMKGNEVIAEAALRAGCRHFFGYPITPQTEISHYMARKM
                     EEIGGVFLQAESEVAAINMVYGAASAGVRVMTSSSSPGISLKQEGISYSACAELPMVI
                     VDIVRCGPGLGGILPAQCDYFQVVKGGGHGDYNLIVLAPASVQELYELTVEAFNLADR
                     YRNPVVILGDGYLGQMMEAVEFRDEEPIEKGNKDWATTGHGGKRIHNVTTSIYIDPNV
                     LEAHNRKLQEKYKKVKENETRAEVVNCDGADVIVVAYGTVARVVKNVIKDAEKLGIKV
                     GLIRPITLWPFPTEVFDKYAGVPKGFLTVEMSSGQMVEDVRLALNGRNRVEFHGRMGG
                     NVPTQKDILDIVRQMADKQ"
     gene            complement(4122..4328)
                     /locus_tag="PLI11_04985"
     CDS             complement(4122..4328)
                     /locus_tag="PLI11_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPZ52252.1"
                     /translation="MARVTFNEDLCKGCKLCIDVCPKKIVVIKDDVLNKKGYHPAGVV
                     EMDKCIGCAFCAMMCPDCVITVEK"
BASE COUNT         1300 a         1131 c          761 g         1341 t
ORIGIN      
        1 atagatttct ctccactttt gaagtgcaat aggatctttt tcgttttcaa aaagccttct
       61 tacagaatgc ctgtaaaggg tgtctccctc attttcaagc tttttgattg tatgaagttt
      121 gtcttttatg gtttttgatt ttctgtagtc agaaaactca taaaccgcct cagttgattc
      181 cttgcagatt ctattgacaa ggtcaataaa ttgctttgtt tcaggtctca tttctgtaat
      241 gttgaacacc cagaaactaa aagccacttc ttcaattttg tcggaaacat catcaatggc
      301 gtttgcaatc aacaatatgt cttctctttc aattggcgtt ataaatgccc tgttaagctc
      361 aaaaacaatt tcatgatata ccttgtcagc gtcatgttct attttgtgta tctcatcaac
      421 tcttttttcc agttgcccgt tcttgttaat atccttgtac tcttctacaa gtgttgtaag
      481 ctcctttgtc agtacgcagg agtagtcaat cgccttcttg aacaaaccaa aataatccac
      541 ttcttttctc ctagccatat attattcctc ctatactagt actatgcgtg attctcttta
      601 tttgtcatgc aaatagtttc aaaaatattt gcgccattac ataaccaata agaccgcagc
      661 ctggaaaagt caggaaccat gccgcaaaca tttctttagc caccgaccaa ttgacattgg
      721 tcaacctttt agcagcaccg acacccataa ttgccgtggt cttcgtatga gttgtactga
      781 ccggaagccc ataaaaatgt gaaagcagca agcaaaaagc agcagcaaaa tcagctgaaa
      841 aaccctcatg cttttccagc ttgaccatgt ccattcctac ggatttgatt atgcggtagc
      901 ctcctattga tgtacccagt cccattatca gtgaacacat gataataagc caaagggcgc
      961 cggataaatc aaactcggtg gatgcggtaa caggcacacc ttgagcgagc attaaaccca
     1021 acatgaatat tcccaagaat ttttgtccat cctgggctcc atgcatgaac gccattgcag
     1081 cacctcctgc aatctggcca cctttaaaga agcggttgga tttccttcta ttcgtacctt
     1141 taaagaaaaa tattattatc ttaacaaaaa tgaaacctac aaaaaagcca agcactgtgg
     1201 aaaagaataa tcctttaatg accttaagcc attcatcatt attgatagct cctttgaaac
     1261 cgtttattgc aaccgcagct cccgtaacac ctgcaatcaa tgcgtgactt tcacttgtcg
     1321 gtatgccgaa ataccatgct ccaaccgccc acacaacgat agcaacgaat gctgcgcata
     1381 gagcaacaag agcggtttct gtatccttcc caaaatcaac caggttatac atcgtcatag
     1441 ctacagaagc attccaaaag gtcatgaaaa gcactccaag aaaattgaat attgcagcca
     1501 ttataattga ttttctcggg cccattgacc tggtggaaat aacggttgca atagcatttg
     1561 gagcgtctgt ccagccattt acgaatataa cgcctattac caataaagac gttattaaga
     1621 gaggtacatt ctttgtgcaa aggcttataa attccaataa actcggcaca gtagcgtctg
     1681 ccaaaaacat cacattcctt tcttagaaac aagttgatat aaatataaca gccttgagat
     1741 ataataatcc caaggcatta ttcttaataa ctcatgccaa attcgagggc tttcatctca
     1801 tccggaataa gattgtgctt gcttggactt aaaacagtcc tcaaagcctc ttcaaaactc
     1861 tttgaagata ttatgcccgt ctcggcaata aatttaccta gcaatataac acctgcaaat
     1921 gcaggatgtc ccatttcgta tgccttctct gtagcaggca gcgaaaaact tctcaaatct
     1981 tgcctttttg gttttattgg aaccaaagca ctgtccatca ctataatacc gttctcttca
     2041 accatgtttt caaatttgtc caaagatggc tgattcatca caattaatgc gttcggacaa
     2101 ctaattatcg gagaaccgat ttcctcgtta gatataataa catggcaatt agccgttcca
     2161 cctctcattt ccggtccgta tgacggcaac caggatacat tatgaccttc atgaagaccg
     2221 cctgctgcca gcagccttcc catggaaaga attccttgcc cgccaaaacc tgatataatt
     2281 atctgttgca tgtcatacct ccaaatcctt acctttgtaa acacccagcg gataatgttc
     2341 cgccatattg tctctcagcc aatccaggga ttttgcagga ctcattcccc aattggtagg
     2401 acaggtggac aatacttcaa ccatggaaaa accaaggcct tccaactgca cttggaaagc
     2461 tttcttaata gctttcttcg cctttattat attctttatc gaatccactg atacacgttc
     2521 tatataaaca gcgccttcca aagttgcaag catttcggat acttttacag gataaccgtg
     2581 aagttgtggt tttctgccaa aaggagatgt ggttgtaacc tgtccaatca gagatgttgg
     2641 agccatctgt cccgatgtca ttccatatat tgcattgttt acaaatatgg ttgttatctt
     2701 ctctcccctt gtggctgcat gcacaatctc tgccgtacca attgcagcca ggtctccgtc
     2761 tccctgatat gtgaaaacaa ccttgtcagg aagagctctc ttcaacccgg tggccacggc
     2821 tggagcgcgt ccgtgagcag cctgcaccat gtcacaactg aagtattcgt agttgttgta
     2881 agtgcagcct actggtgaca caccaactgt ttccccttct atatcaagtt catctatcac
     2941 ctctgcgaca agcctgtgaa ttatgccgtg cgtgcagccg gggcaatagt gaaacggttt
     3001 gtccgtcaat gcatttggtt ttttaaaaac tatagccatt gtacaccccc tactgtttat
     3061 ccgccatctg gcgcactata tccagaatgt ccttctgcgt aggaacattt ccgcccattc
     3121 ttccatggaa ctctaccctg tttcttccgt ttagggcaag ccttacgtct tccaccatct
     3181 gacctgaact catttcaaca gtcaggaaac ctttaggcac gccagcatac ttgtcaaata
     3241 cctctgttgg gaaaggccat aatgtaatag gacgaataag tcctacctta atacccaact
     3301 tttcagcatc ttttatgaca ttcttaacga cccttgctac cgttccataa gcaacaacaa
     3361 taacatccgc accgtcacag ttaacaactt cggcgcgggt ttcgttttcc ttaacctttt
     3421 tatacttttc ctgcagtttt ctgttatgag cttcaagaac attcggatct atataaattg
     3481 aagtggttac gttatgaata cgctttccac catgaccggt tgtagcccaa tccttgtttc
     3541 ccttttcaat tggctcttca tccctgaact caaccgcttc catcatctgg ccaagataac
     3601 cgtctcccaa tattactaca ggattcctat aacggtcagc cagattgaac gcttcaacgg
     3661 ttaattcata caactcctgt acgcttgcag gagccagaac tataaggttg tagtcgccat
     3721 gacctccacc ttttacaacc tggaaatagt cacactgtgc cggtagtatg ccgcccagac
     3781 caggaccgca ccttacaatg tccacaatta ccatgggcag ttccgcacac gcggaatatg
     3841 aaataccttc ctgtttcaag ctgatgccag gacttgaaga agacgtcata acccttactc
     3901 cagcactggc tgctccgtac accatgttta ttgctgcaac ttcgctttct gcctgcagga
     3961 agactccacc tatctcctcc attttccttg ccatgtaatg ggagatttca gtctgaggag
     4021 ttataggata gccaaaaaaa tgcctgcagc ccgctctaag cgcagcttca gcaataactt
     4081 cattaccttt cattaaaatc ctttttccca tcttttcctc cctacttttc cactgtaatt
     4141 acgcaatcag gacacatcat cgcacagaaa gcacatccta tgcacttgtc catttcaacg
     4201 acgcctgctg gatgataacc ctttttattc aaaacatcat ccttgataac aactatcttt
     4261 ttggggcaga catctatgca taatttacag cctttacaca aatcttcatt aaaagttact
     4321 ctagccatat caacatcacg ataagtagtg atttgccccc tctcaaacaa catggatatg
     4381 taaggataaa gttaaacttc atcctttcaa ttatactact taaaaaaatg aattgcaagg
     4441 aatgaaaaaa cggttaaagt caaaaaaaca caaccaaaca aatgcattta caataatcat
     4501 tggtaatgat atgcttaaaa cagttaaacc gac
//
LOCUS       DAONVY010000141         4519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13738_length_4519_cov_4.381496, whole genome shotgun sequence.
ACCESSION   DAONVY010000141 DAONVY010000000
VERSION     DAONVY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13738_length_4519_cov_4.381496"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="PLI11_04990"
     CDS             complement(<1..2158)
                     /locus_tag="PLI11_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ52253.1"
                     /translation="MHNLDSIVLSPIPLGDIEAKGWLLNQLLLQKKNITGEFEDISPD
                     VKTTGDDRSGWLGGSGESWERGPYYVRGLVYLAYALDDDSLKEKVQKWIDWVIESQID
                     NGQAGPKAGMPDFDYWAMMPMLIALTEYYDATEDERVIGFLDAYFRFQLDALKKDPLR
                     DWGKARAGDNIYAAKWLYDKTKESYLVDLCNLLYNQADNWKSAYEKGNWGHIVNTHQS
                     FKLFPLMFGMTGDKTYVDTYYSGVRNLFRTSGRIDGMSNGDEMSGDIEATRGTETCAV
                     VEQMFSDIVAFRYLKDPRIGDSLEKVCFNALPSQLTPEITGQVYFTMQNQIEATLGYH
                     GFSSDGGDRSVYGTPGGYPCCIHNFHMGWPLFASNLWFRTSDSGLLAFAYAPNAVKAQ
                     VGNNTNVMITQKTNYPFEDSILLKIEADNEETWPLYIRIPSWCDEAKIYVNGDFKGSC
                     KEGSFVRLEAEWKSGDEVKIVLPMKIRTKFMQNNSVGVTYGPLVFCLFIEENWEKISY
                     VSTDWKIPEKYSSYNITPKSNWNYALNLSEGFKVKRKKRYDYDNLVFKSVSVPIEIEA
                     KGAIVESWEYDDRIKGASTLPISPVSEHGKEVKIRLIPYGFARLRASLMPWYGKEKVL
                     YEPDFNVFDTVVAPVDKNDENARLGALPLGKYTGLIRCDVEKDSSIDVLVNGEIVDSV
                     SLYKGEEYFSLPLPIMQNMYNRVEFKGDVSSVKIKSL"
     gene            complement(2250..2900)
                     /locus_tag="PLI11_04995"
     CDS             complement(2250..2900)
                     /locus_tag="PLI11_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52254.1"
                     /translation="MKRIVVLIIAILMFSILAHAEEGDRYEAELASIYMCNNAGDHVA
                     QIDNAGAYVEFILDETTAPGVYKLRIYYAAALEGNNSHTLFIDDEEIERIMYPQSELG
                     WSTFSEDVYVETEVTLPTGVCTLRLEKTEEDEGFAELDAIRLIPVTLYTPEPTPEPTE
                     TPIPTKTEAPKPTKTQETTTEEKDSFNTLFVVVPAAVVVLAALVIVLIVKKKGHKN"
     gene            complement(2996..3793)
                     /locus_tag="PLI11_05000"
     CDS             complement(2996..3793)
                     /locus_tag="PLI11_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52255.1"
                     /translation="MENIIQLEIPPYPMFRISAFREFEEGEWHITRKNMEYILIIMVE
                     GKLYFTENGREVELNKDEFYIQRPNIYQSALRPSKTPKYYYIHFTQEVSDYTGYFPAK
                     TLILPTRGKCKVHVYRNYLDRIAYAHLDSASDILEVQSLFLDFLQFFYENEIEQPKCN
                     AGTVLLDYMRKNYQSHITLKTLSELVNFSEDYTRSIFKKRYGIAPIEYLAKIRLEMAC
                     KLLCTTDYSIEKIAYDVGFGDVSVFYRNFVKAFNMAPSKWRSMSRNM"
     gene            3937..>4519
                     /locus_tag="PLI11_05005"
     CDS             3937..>4519
                     /locus_tag="PLI11_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52256.1"
                     /translation="MKRAVVFLVILLVLVQFAIAVNANEILPATVNALKFDAEPTFDG
                     VITEEEWGEPTLKDVVNGNPEQVFLTAGAPELTWTAWLRWDSNYFYLALQTPDTKHSN
                     RQEGANLWGGDCVQLKIDPLGNWQSQGQTDKTYKSNKAVDVVFALSSKTNQSVSHCWN
                     EGKNVIQGAKVAVKHENNMTSYEIAIPWAFLEVD"
BASE COUNT         1292 a         1050 c          780 g         1397 t
ORIGIN      
        1 caagactttt tatctttacc gaagacacat ctcctttaaa ctccactctg ttgtacatgt
       61 tttgcattat tggaagcggc agcgagaaat attcctcacc cttgtataag gaaacactat
      121 caacaatttc cccatttaca agaacatcaa tacttgaatc cttctccaca tcgcacctta
      181 tcaatcctgt atatttgcca agaggcaatg caccaagcct tgcattttcg tcatttttgt
      241 caacaggtgc aaccacagtg tcaaaaacat taaaatcggg ctcatacaaa accttttctt
      301 taccgtacca gggcattagt gaagccctta acctggcaaa accataaggt attagtctaa
      361 ttttaacctc tttgccatgt tccgaaacag gacttatcgg caatgtggat gcacccttta
      421 tcctgtcatc atattcccag gactccacaa ttgctccctt tgcctcaatt tcaatcggaa
      481 cactgactga tttaaacaca agattgtcat agtcatatcg tttttttctc tttaccttaa
      541 aaccttcact taaattcagt gcatagttcc aattgctttt tggcgttatg ttgtaggaag
      601 aatacttttc aggaatcttc cagtcggttg acacataaga aatcttttcc cagttttcct
      661 cgataaacaa gcagaaaaca agaggtccgt atgtaacccc aacactgtta ttctgcatga
      721 actttgtcct tatcttcatc ggcagtacga tcttaacctc gtccccggac ttccactctg
      781 cctcaagcct tacaaatgaa ccctctttac aactaccctt gaagtctcca tttacatata
      841 ttttagcttc atcacaccat gagggaatcc taatgtacag aggccatgtc tcctcgttat
      901 ccgcctcgat ttttaataat atgctgtctt caaaaggata attggttttc tgcgttatca
      961 tcacatttgt attattacca acctgggctt ttacagcatt tggagcatat gcaaacgcta
     1021 aaagcccgct atcacttgtt ctgaaccaca agttggatgc aaaaagcggc caacccatat
     1081 ggaagttgtg tatgcagcag ggatacccgc caggtgttcc atacacagat ctgtctccac
     1141 cgtctgaaga aaagccatga taccccaagg ttgcttctat ctgattctgc atggtgaaat
     1201 atacctgccc agttatttct ggtgtaagct gtgacggcag tgcattgaag caaacctttt
     1261 caaggctgtc gcctatcctt gggtctttca aatatctgaa tgctacaatg tcgctgaaca
     1321 tctgctcaac aacagcacag gtttccgttc ctcttgtcgc ctcaatatct cctgacattt
     1381 catcaccatt tgacattcca tctattctac cgctagtcct gaacagattc cgaactcctg
     1441 aataatatgt atccacataa gttttatctc ctgtcattcc aaacataagc ggaaacaatt
     1501 taaagctttg atgggtgttg actatgtgtc cccaatttcc cttttcgtag gcagacttcc
     1561 aattatcagc ctggttatac aaaaggttgc acaaatcgac aaggtacgat tccttcgttt
     1621 tatcatacag ccatttagcc gcatatatgt tgtcaccagc tctagccttt ccccaatccc
     1681 tcaatggatc tttcttcaat gcatccagct ggaatctgaa atatgcatcc aaaaagccaa
     1741 ttaccctttc atcttctgtg gcatcataat attctgttaa tgctataagc atgggcatca
     1801 ttgcccagta gtcaaaatcc ggcatgcctg ccttgggtcc tgcctgtcca ttatcaatct
     1861 gactttctat cacccagtct atccactttt gcactttttc tttaagtgag tcatcatcaa
     1921 gggcgtatgc aagataaaca agacctctta cgtaatacgg gcctctttcc cagctttccc
     1981 cactacctcc aagccaacca gaacgatcat ctccggtggt tttcacatcc ggagatatgt
     2041 cctcaaactc tccagtaata ttcttcttct gcaaaaggag ctggtttaaa agccagccct
     2101 ttgcctcaat atcgcccaaa ggaatgggcg aaagcactat actatccaaa ttatgcatgg
     2161 aaaaactaac ctctctattg tctttattta taaacaagac caaaatcaag gatacactta
     2221 aaacacagat tatcaaaact aaaagagttt tagtttttat gccctttttt tttaacaata
     2281 aggactatca cgagagcagc caaaacaaca actgcagcag gaactacaac gaacaaagta
     2341 ttgaaggaat ctttttcctc agtagtggtt tcctgtgttt ttgtaggttt gggcgcttct
     2401 gttttagttg gaattggcgt ttctgttggt tccggtgtag gctccggagt gtacaaagta
     2461 acgggaataa gtctgattgc gtccagttct gcaaaccctt catcctcttc cgttttttca
     2521 agccttaatg tacatacacc tgtcggaagt gtaacttctg tttccacata tacatcttct
     2581 gaaaaggtag accaacccag ttcagactga ggatacatta tcctttcaat ctcctcatcg
     2641 tcaataaata gtgtgtggga attgtttccc tcaagggccg ccgcatagta gattctcagc
     2701 ttgtaaacac ccggagcggt tgtctcatca agaataaatt caacatatgc acctgcattg
     2761 tctatctgcg ctacatgatc accagcattg ttgcacatat aaatagaagc cagttccgcc
     2821 tcatacctgt ctccttcttc tgcatgggcc agaattgaaa acataaggat tgcaataata
     2881 agtacaacta tccttttcat ttatcaacac ctccatcaac ttatgttaat tctagcatta
     2941 attcattctt catgaaactg cagaaattgg gaaaaatcgt gcaatttccg aacagttaca
     3001 tgtttcttga catgcttctc cacttggaag gcgccatatt aaatgcctta acaaaattcc
     3061 tgtaaaatac tgaaacgtcc ccaaatccca catcgtaagc tattttctca atggaatagt
     3121 ccgtagtaca tagtaatttg catgccatct caagtctgat ttttgcaaga tactcaatag
     3181 gtgctatacc atatcttttt ttgaatatgc ttcttgtgta atcctcggag aagttgacaa
     3241 gctctgacaa agttttgaga gttatgtgtg attgataatt ctttctcatg taatcgagca
     3301 aaaccgtacc tgcattgcat ttgggctgtt ctatctcatt ttcataaaag aactgcaaga
     3361 aatcaagaaa cagagactgc acttccaaaa tgtcacttgc actgtctaga tgagcataag
     3421 caattctatc aagatagttt ctatatacat gaactttaca ctttcctctt gttggcagta
     3481 tcaaggtctt tgcaggaaaa tatccggtat aatcagaaac ttcctgggtg aagtggatgt
     3541 aataatattt gggggtttta ctgggcctca aagcgctttg atagatgttt ggtctttgta
     3601 tgtagaattc gtccttgtta agttccacct ctctgccgtt ttcagtaaaa tacaattttc
     3661 cttccaccat tattattaaa atatactcca tgttttttct tgtaatgtgc cactctccct
     3721 cttcaaactc ccgaaaggca gatatcctga acatgggata tgggggaatc tcaagttgta
     3781 taatattttc catacatttc ctccaatgtt ccgaattata tacatttctt tttaatatta
     3841 taccatattc tttcatattt tttgtcatag catgtttaca tttccatcat tataacatat
     3901 aatctaactg ttaataccga tatggaggag atacatatga aaagagctgt tgtatttctt
     3961 gtaatcttgc tggtccttgt gcagtttgct attgcagtaa atgcaaatga aattctgcca
     4021 gcaacagtga acgctcttaa gtttgacgca gaacctacct ttgacggtgt catcaccgaa
     4081 gaggaatggg gagagcctac actgaaggat gttgtcaatg gtaatcctga gcaggtattt
     4141 ttaaccgcag gcgctccaga acttacttgg acagcctggc tcagatggga ctccaactat
     4201 ttctatctgg cactgcaaac acctgacacc aaacactcca acagacagga aggtgcaaat
     4261 ctttggggcg gcgactgtgt ccagttaaag atcgatcctt taggaaattg gcaaagtcag
     4321 gggcaaaccg acaagactta caaatccaac aaggcagtag acgttgtctt tgctctcagc
     4381 agcaaaacca accaaagtgt aagccattgc tggaatgaag gaaagaatgt aatacaaggt
     4441 gccaaagtcg ctgtaaaaca cgaaaacaac atgacatcct acgagattgc aattccttgg
     4501 gcattcctgg aggttgatg
//
LOCUS       DAONVY010000142         4509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13783_length_4509_cov_3.550741, whole genome shotgun sequence.
ACCESSION   DAONVY010000142 DAONVY010000000
VERSION     DAONVY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13783_length_4509_cov_3.550741"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2792)
                     /locus_tag="PLI11_05010"
     CDS             complement(<1..2792)
                     /locus_tag="PLI11_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HPZ52257.1"
                     /translation="MKEKNLTEKAFTLLSEKILDHEAISKVRELSRDNGEPFVRFFKC
                     ADFLKDYRFYNSYKGRPDGLGSIRAFEDMTESNLYHYVYWRTQFLENNYKQTKREYIL
                     VYINELVNLVGVTSEEGLALLIDLWNKCDELTDLEFYDNIKDYCILHSVGSFKDIVKS
                     FDEKFQIASYEDKIYSGDYEHTLPYFDKMSAQHQIYGSSFYRSEKGRYIDSVFEVVMK
                     GVSEYFSQKGISFNEMLFERKVDVYEPFKYNPFVKKDYGFSEYVISETERYFVQDNIL
                     YAEKLFPVRKHARLISFILKHMESRIREILGEKPLKIYDTSLYQDEEMRSIIDTGELV
                     KVIHKQIAIVMHEEVAERNRQIRIKDKFDKMRSLSRTPKGMIYGAKLFLKQVKFMEKV
                     EYNEDVVLVDFLNVYPTYTHMTNQQLLGYFSWRTKIRKGIYEKADIGYIKLYLNETIL
                     LANCIDELDTLSKLLDFWKGMRNVTDIDKLMEDTIIEFYVLKGLDMPFAEVCERFPEG
                     YIGNTLIMDEIAHGNYTNVYEYFNRRIRIRVSKEVQSVLKRVLPVALKEASDLLEREC
                     AFSLGSLLLGNVIKEEVSFSQLGYLIDKKECRIKEETYINKGIKYTFENGSLFYYGCD
                     FDKREQKFCEYVVNIIATAARRYKKGDFSDIPFDIIFSGLERYSKHAEIIESMRFVHI
                     LTNEVKKIYKDVKIKEKQRLESFVLGGKKRYYDPEVVQQNKHLLSLVSSEAKEQRISS
                     RQTYRYDYYNPIDFYTQACLLEDVDVYEKQRVSHDYDFANRYYLNWRSDVRRGIFKPA
                     NKNMVLLYLSELVNLIGVENEEELMDKLIKFWHGYSQVSDNIDDLIESVIMDAYIAYD
                     LDKPYSYYAEKFPKKDYTVYGLYKIFKGDYDCASMFILEKSSYRARESAFGRSGNGHL
                     IDLCMPKVLEKLDELFS"
     gene            complement(2789..3607)
                     /locus_tag="PLI11_05015"
     CDS             complement(2789..3607)
                     /locus_tag="PLI11_05015"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPZ52258.1"
                     /translation="MKTHKITAILVLIALLITGCANSNVPADETTEPTLTDNIDKTPG
                     EGEGPTPSPTEVLPLHGLKICIDPGHQGKANNELEEMAPWSSEKKRKIAAGTKGATTG
                     TPEYMLTLKIGLKLRDALMNAGAEVLMTRETHDVDISNKERALMSNEFGSHLTLRIHL
                     NGVDNKDVHGMEIYIRDKGDGSEETKALANYELAIANMLMEKLVNATGAANRNVRRSD
                     NYTSINWTEGPCMILELGYLTNEEEEKKLVSDEYQDTIVNAIVEFLKENDVTKR"
     gene            complement(3624..>4509)
                     /locus_tag="PLI11_05020"
     CDS             complement(3624..>4509)
                     /locus_tag="PLI11_05020"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ52259.1"
                     /translation="KEYVSCCMFALSTMYMRDMARAIGSEKEVEYAKLYEDIKKHFQD
                     KFLDENGKLKETSQTAYLLALKTYMLPESYVQEAADCLEQKIKDNGYRLSTGFIGTGI
                     LNQTLSEFGKSNIAYSLLLQTEDPSWLYSVYQGATTIWERWNSYTIEKGFGDAGMNSF
                     NHYAYGAVLEWMFKYMAGINNDFNEPGFKHVVLRPMPDTRTKDELPEGQENITYVEAE
                     YESASGLVKSSWRMENGTFVYNCTVPTRASLCFPLINKDENSTIRINDKVALVSEFER
                     AGNRIRINLDEPGSYTIV"
BASE COUNT         1219 a         1050 c          726 g         1514 t
ORIGIN      
        1 gaaaagagtt cgtccagttt ttcaagcacc tttggcatgc agagatctat aagatgacca
       61 ttaccacttc ttccaaaggc actctctctt gccctgtagg aacttttttc aagaatgaac
      121 atgctcgcac aatcatagtc acctttaaaa atcttgtaca gaccgtatac tgtataatct
      181 ttcttcggaa acttttctgc ataatatgaa tatggtttat ctaaatcgta ggcgatgtat
      241 gcatccatga tgacactctc gatcaagtca tcgatgttat ccgaaacctg gctgtatcca
      301 tgccagaatt ttatcagctt gtccatcaat tcctcttcat tctcaactcc tatcaaattg
      361 acaagctcgg acaaatacaa aagaaccatg tttttgtttg caggcttgaa tattccccgc
      421 cttacatctg atctccaatt caggtagtaa cggtttgcaa aatcataatc atgagagact
      481 ctctgtttct catatacatc aacatcttca agaaggcatg cctgagtata aaaatcaata
      541 ggattatagt agtcatacct ataagtttgc ctgctggaaa tcctttgttc tttagcttca
      601 gaacttacca atgaaagcag atgcttgttt tgttgaacta cttcaggatc ataatatctc
      661 ttttttccac caagaacaaa gctttccaat ctttgctttt cctttatctt cacatccttg
      721 taaatctttt taacctcatt ggtcaaaata tgcacaaatc tcattgattc aatgatttct
      781 gcatgttttg agtatctttc cagtcctgaa aagataatat caaaaggaat atctgaaaaa
      841 tctccttttt tgtaccttct tgctgcagtg gcgatgatat ttacgacata ctcgcaaaac
      901 ttctgttccc tcttatcaaa atcacagccg taatagaaca aggaaccatt ttcaaaagta
      961 tacttaatcc ccttgttaat atatgtttcc tctttaatcc tgcactcctt tttatcaata
     1021 aggtatccta gttgcgaaaa ggacacttct tcttttatga cattacccag caatagactt
     1081 ccaagcgaaa atgcgcattc cctttcaagc agatctgacg cctccttaag agcaacggga
     1141 agaacccttt ttagaacact ctgaacttcc tttgaaacac gtattcttat ccttctgtta
     1201 aaatactcat atacatttgt gtaattgcca tgagcaatct catccataat caaggtattg
     1261 ccaatatacc cttcaggaaa cctttcgcaa acctctgcaa aaggcatgtc caatccttta
     1321 aggacataga actcaataat agtgtcctcc attagtttat caatatcagt gacattcctc
     1381 atacctttcc agaagtctaa aagttttgaa agagtatcca gctcatcaat acaatttgca
     1441 agaagtattg tttcgttcag atacagttta atatatccga tatccgcctt ttcataaatc
     1501 ccttttctaa tctttgttct ccatgagaaa tagccaagca gttgctggtt tgtcatatgg
     1561 gtatatgtag gatatacatt taaaaaatcc accaatacca catcttcatt gtactcaacc
     1621 ttttccatga atttaacttg ttttagaaat aacttcgcac cataaatcat tcctttggga
     1681 gttcttgaaa gagatctcat cttatcgaac ttgtctttaa tccgaatctg cctgttgcgt
     1741 tcagcaactt cttcatgcat aacgatagct atttgcttat ggattacctt aaccagttct
     1801 cctgtatcta ttatgctgcg catctcttcg tcctggtaaa gactcgtatc atatattttc
     1861 aagggttttt caccaagaat ttcccttatt cttgattcca tatgcttaag tatgaaagat
     1921 atgagtcttg catgtttcct gacagggaag agcttttccg catacaggat gttgtcttgt
     1981 acaaaatatc tttccgtttc tgatataacg tactcactga atccatagtc ctttttcaca
     2041 aatggattgt atttgaaagg ctcgtaaaca tccacttttc tttcaaacag catttcattg
     2101 aacgaaatac ccttctggga aaagtattca ctcacacctt tcataacaac ttcaaaaacg
     2161 ctgtcaatat atcttccttt ttcacttctg taaaaagagc tgccataaat ttggtgctgg
     2221 gcactcattt tgtcgaaata cggaagagta tgttcataat caccggaata tattttgtcc
     2281 tcgtaagacg ctatttgaaa cttttcatca aaactcttga ctatgtcttt gaaagaacct
     2341 acggaatgaa ggatgcagta gtctttgata ttgtcataaa actcaagatc agtaagctcg
     2401 tcgcatttgt tccataaatc aatcagaagg gcaagtcctt cctcgcttgt tacacccacc
     2461 agattgacaa gctcgtttat gtatacaaga atgtattctc gttttgtttg cttgtaattg
     2521 ttctccaaaa actgtgtgcg ccagtacacg tagtgataga gattggattc cgtcatgtct
     2581 tcgaaggctc taatgcttcc aagaccatcc ggtctgccct tgtacgaatt ataaaagcga
     2641 tagtccttta aaaagtctgc acatttaaaa aaacggacaa aaggctcccc attgtccctg
     2701 gataattcac gtaccttgct gattgcttcg tgatccagta tcttttctga caacaatgtg
     2761 aaagcctttt ccgttaaatt cttttccttc atcttttggt tacatcattc tccttgagaa
     2821 actctacaat tgcattcaca attgtatcct gatattcgtc acttacaagc ttcttctctt
     2881 cctcttcatt ggtcaaatat ccaagctcga gtatcataca cggaccttct gtccagttga
     2941 tacttgtata attgtcgctt cttcggacat ttctatttgc ggcccctgtt gcatttacaa
     3001 gcttctccat aagcatgttt gcaattgcaa gctcatagtt ggcaagtgcc ttggtttcct
     3061 cactgccgtc acccttgtcc ctaatatata tctccatgcc gtgtacatcc ttgttgtcca
     3121 ctccattcaa atgaatcctg agtgtaagat gggaaccgaa ctcatttgac ataagagctc
     3181 tttccttgtt tgatatgtcc acgtcatgag tctctcttgt cataagtacc tctgcgcctg
     3241 cattcatcaa agcatcacgc agcttaagac caatcttcaa agtcaacata tactcaggtg
     3301 tacctgtagt tgcacccttt gtacctgccg caatctttct ctttttttca ctgctccacg
     3361 gagccatttc ttcaagttca ttgttcgctt tgccctgatg accaggatca atgcatatct
     3421 tgagaccatg caaaggaaga acttctgttg gagaaggagt tggtccctcc ccttctcccg
     3481 gtgttttatc aatgttatca gttaatgtcg gttccgttgt ttcatctgct ggtacgttgc
     3541 tgtttgcaca tcctgtaata aggagtgcaa tcaataccaa aattgctgtt atcttatgtg
     3601 ttttcaactc gtactactcc ttcttataca atggtatagc tgcccggttc gtcaaggttg
     3661 attcttatac gattgccagc cctttcaaac tcagatacga gagcaacctt gtcatttatt
     3721 cttatcgtag aattttcgtc cttgtttata agggggaagc acaatgaagc cctggttggc
     3781 acagtgcagt tataaacaaa ggttccgttt tccattctcc aactgctctt tactagaccg
     3841 ctggcagact catactccgc ttcaacatag gtgatgttct cctgaccttc aggcaactca
     3901 tcttttgttc tggtatcagg cattggtctt aaaacgacat gtttgaaacc aggctcgtta
     3961 aagtcattgt taatacccgc catgtacttg aacatccact ccaacacagc accgtatgca
     4021 tagtggttga aggagttcat tccagcatct ccgaatccct tttcaattgt atatgaattc
     4081 catctctccc aaattgttgt tgctccctga tatacgctgt acagccagga tggatcttcc
     4141 gtctgcagaa gcaacgaata cgctatattg ctttttccaa actcactcaa tgtctggttc
     4201 aatataccgg ttccgataaa acctgtactc aacctgtatc cgttgtcctt tatcttctgc
     4261 tccagacagt ccgctgcttc ctgaacatag gattctggca gcatgtatgt ctttagagcc
     4321 aggagatatg cagtctggga tgtttcctta agctttccat tctcgtcaag gaatttatcc
     4381 tggaaatgct tcttgatatc ctcatatagc tttgcatact ctacttcctt ttccgaacct
     4441 atagcccttg ccatgtccct catgtacatg gtgctcaagg caaacatgca gcatgagacg
     4501 tattccttg
//
LOCUS       DAONVY010000143         4492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13840_length_4492_cov_3.861393, whole genome shotgun sequence.
ACCESSION   DAONVY010000143 DAONVY010000000
VERSION     DAONVY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13840_length_4492_cov_3.861393"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLI11_05025"
     CDS             <1..559
                     /locus_tag="PLI11_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPZ52260.1"
                     /translation="AIVIHKMPGIMRNSTIKVANIPMLNAEIVTGIAFMLFFGAISVP
                     LGFSTILIAHITFNVPYVILSVLPKLRQLNKSTYEAALDLGATPAMAFRKIVLPDILP
                     GIISGLLIAFTLSLDDFIISYFTAGAAFKTLSVEINTLTKRRIPLSVNALSTIIMIVV
                     FVILVINNLRKRPNGNKALIKERSA"
     gene            561..1616
                     /locus_tag="PLI11_05030"
     CDS             561..1616
                     /locus_tag="PLI11_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ52261.1"
                     /translation="MKKTVRIVSSILLVLSLVGVIFLTGCKDDRETLYVFNCGDYIDE
                     RVLDMFMEEYPHIRIVYDTFDTNEIMYQKLINSKEPYDVLIPSDYMIERLIKEDRLHK
                     IDFSKLTNYDKIDDSFRGLAYDPDEEYSVTYMWGTLGILYNKKLVTEPVNSWSILWDS
                     KYKGKILMLDSMRDTMGVALRKLGYSMNSTNLDEIKAAKEALIDQIDLVADYGVDKLK
                     DMMIAGNYALMVAWSGDAVWMMNENSDLAYAVPDEGSNIWADAMVISKTGKNIEAAHL
                     FIDFMCRTDIAKMNAEYIQYSTPQKEALSQLDASLRESEVYNPPKSVIERCESFIDLG
                     DAVKYYNEAWEEVRLHG"
     gene            1631..2119
                     /locus_tag="PLI11_05035"
     CDS             1631..2119
                     /locus_tag="PLI11_05035"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPZ52262.1"
                     /translation="MASKNVKKLVYHSALAAMIIVATAFMKIAVPVYGGYIHMGDGFI
                     FTAAFLLGPMAFVPAAIGSCIADLILGYALYAPATFVIKGLMGLFAGFALKDGNKPAR
                     NLVVFILAEIIMIAGYFFYELLIYDGIAPALLGIAYNGIQGLCGVVMGMLFIPVAKRI
                     KL"
     gene            complement(2215..2343)
                     /locus_tag="PLI11_05040"
     CDS             complement(2215..2343)
                     /locus_tag="PLI11_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52263.1"
                     /translation="MSCFGLDDDCILWIILLLALLGILNDCNRRGVLGTRDKDCCC"
     gene            complement(2411..3166)
                     /locus_tag="PLI11_05045"
     CDS             complement(2411..3166)
                     /locus_tag="PLI11_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPZ52264.1"
                     /translation="MLNIYIYDGSYHGLMTSCFDAWKDKDIYSIRTKEENKDVNLLEK
                     VVYIETDNEKAERIIKWVGKEACYEVQRKLYRVFLTEHPERGMYIFRYLKLLNQLGRN
                     VENHLYNDDVRYINKLSRMHASETHRLYGFVRFEVIGNNILYARIEPDFNQLEVIAPY
                     FADRYNGHMWIIHDLARNMAAIFNGSQYIITEYNGNMLDNESKTEISFQKMWVEYLDA
                     LTIKERLNLDLQKQKVPLKTRKHLTEFMFKSKN"
     gene            complement(3160..4485)
                     /locus_tag="PLI11_05050"
     CDS             complement(3160..4485)
                     /locus_tag="PLI11_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HPZ52265.1"
                     /translation="MNILGAAAKYDASCASSGVERRGSSKQGAMGNSVACGICHSFTQ
                     DGRCVSLLKVLQSNDCVYDCKYCVNRRTNDVERATFMPREIAMLTYEFYRRNYIEGLF
                     LSSAVILSPDNTMEIMYETLRLLRQEFCFYGYIHVKAIPGADPLLIERAGRLADRMSV
                     NIELPSEASLTRLAPQKKKRSIFSSMRYISGRIEEDKSGKFLLRDNLPAPLGETMPTS
                     YGTGSSNKRTFVPGGQSTQLIIGATDDTDHDILTLTENLYNKMQLKRVYYSAYVPAVK
                     DPSLPSLAEPPLLREHRLYQADWLLRFYGFRSSELLNERNPNLNLKYDPKCDWAVRNL
                     DLFPVEVNKADYDTLLRVPGIGTISARKIVSARKVGSLGFEELKKMGVVLKRAQYFIT
                     CRGKMMPHIKFDPKFIDANLSLDKRAIPIFSNYEQLSLLDYINGGDSLC"
BASE COUNT         1231 a          884 c          960 g         1417 t
ORIGIN      
        1 tgcaattgtc atccacaaga tgcccggcat tatgagaaac tctacgataa aggttgccaa
       61 catcccaatg cttaatgcag agattgtgac aggtattgcg ttcatgctgt tttttggtgc
      121 cataagtgtt cctttgggtt tttcaacaat acttattgca cacattacct tcaatgtgcc
      181 gtatgttata ctatcagtac tgcctaaact gaggcagttg aacaaaagta cttatgaagc
      241 ggcgttggat ttgggagcta cacccgccat ggcctttagg aagattgttc ttccagacat
      301 actgcctggt atcatatccg gccttttgat tgcgtttaca ctgtcgcttg atgacttcat
      361 tatcagctat tttacagcgg gagccgcatt caaaactctc agtgtggaga taaacactct
      421 gacaaagaga aggattcctt tgtctgtaaa tgcgctgtcg acgattatta tgatcgtggt
      481 atttgtaata ctagttatta acaaccttag aaaacgacct aatggaaata aagctttgat
      541 taaggaaagg agtgcttgaa atgaaaaaga cagtaagaat tgtcagttca attttgttgg
      601 ttttgtccct tgtaggagtc atattcctga caggctgcaa ggacgaccgt gaaacacttt
      661 atgtgttcaa ctgtggagac tacattgacg agcgtgtcct cgacatgttc atggaggagt
      721 atccgcacat aaggatagtt tatgatactt ttgatacaaa cgaaatcatg tatcagaaac
      781 tcataaacag caaagaacct tatgatgttc tgattccatc agactacatg attgaacgtc
      841 tcataaagga ggataggctc cataaaattg atttttccaa gctgaccaac tatgacaaaa
      901 ttgacgactc cttcagaggg cttgcgtacg atcctgatga agaatactca gtaacatata
      961 tgtggggaac attgggaatt ctatacaata aaaagctggt taccgaacct gttaacagct
     1021 ggtccatact gtgggattca aagtacaaag gcaagatttt aatgcttgac agcatgagag
     1081 acaccatggg tgtggccttg agaaagcttg gttattcaat gaattccaca aatcttgatg
     1141 aaattaaggc tgcaaaagaa gctttgattg accagataga tctggtggct gattatggtg
     1201 ttgacaagtt gaaggatatg atgattgcag gcaattatgc ccttatggtg gcgtggtccg
     1261 gagatgccgt atggatgatg aatgagaatt ctgatttggc gtatgccgta cctgatgagg
     1321 gcagcaatat atgggctgat gcaatggtca tatcaaaaac aggcaagaat atcgaagctg
     1381 cacacctgtt tattgatttc atgtgcagaa cggatattgc gaagatgaat gctgaatata
     1441 ttcagtactc aactccacag aaggaggctt tgagtcagct tgatgcaagt cttagggaaa
     1501 gtgaggttta caatcctcca aagtcagtta ttgaaagatg cgaatcgttt attgatttgg
     1561 gtgacgctgt gaaatattac aacgaagctt gggaggaagt caggcttcac ggttaaaaag
     1621 gaggtcttgt atggctagca aaaatgtaaa aaaacttgtt tatcacagtg ctttggcggc
     1681 aatgattatt gtcgccacag cattcatgaa gatcgcagta cctgtttatg gtggctatat
     1741 acatatgggt gacggtttca tatttactgc agcttttttg cttggaccca tggctttcgt
     1801 accagcggca attggttcct gcattgcaga tttgattcta ggttatgctc tatatgcgcc
     1861 agcgacattt gttattaaag gtttaatggg attatttgca ggatttgccc tgaaagatgg
     1921 caacaagcca gcaagaaacc ttgtggtatt catacttgct gaaatcatca tgattgccgg
     1981 atacttcttt tatgagctgt taatttacga tggaattgcc cctgcattat taggcatcgc
     2041 atacaatgga attcaaggtt tgtgcggagt ggttatgggt atgcttttca tacctgtggc
     2101 aaagagaata aaactttaga gttatatgag ttgatacgtc aaaaatgggg ctgtcgcata
     2161 agcgatagcc cctgactcat tcgctgccat caattagtta gctaattaat caaattagca
     2221 gcagcaatcc ttgtctctgg tgcctaacac acctctacga ttacagtcat tcagtatacc
     2281 caacagggcg agaagtaata tgatccaaag gatgcaatcg tcatcaagtc cgaaacaact
     2341 cattgtttaa cctccttgtg ctccatctac attacaatat cattgtatgg agaagagaga
     2401 aaatgtgtga ttaattttta cttttaaaca taaattctgt taaatgcttt ctggttttca
     2461 gcggaacttt ctgtttttgc aaatcaaggt tcagtctttc tttaattgtc aaagcatcca
     2521 aatattcaac ccacattttt tgaaaggaga tttccgtctt tgattcatta tcaagcatat
     2581 ttccgttata ctctgttatg atatattggc taccattgaa gatagcagcc atattccttg
     2641 caaggtcgtg tatgatccac atgtggccgt tgtacctgtc tgcaaagtag ggggcaatga
     2701 cttccaactg gttgaaatca ggctctattc gtgcatagag tatgttgttg cctattactt
     2761 caaaccttac aaatccataa agcctgtggg tttctgatgc atgcattctg ctcagtttgt
     2821 ttatatatcg cacatcgtca ttgtacaggt ggttttcaac atttctaccc agctggttaa
     2881 gaagttttag atatctgaag atatacatgc ccctttcagg atgttctgta agaaagactc
     2941 tgtagagttt tctttgaacc tcataacaag cttccttgcc aacccatttt atgattctct
     3001 cagctttttc attatctgtt tcgatatata caactttttc aagcaggttg acatccttgt
     3061 tttcttcttt tgttcgaatt gaatatatgt ctttatcttt ccatgcatca aaacaactgg
     3121 tcataagacc gtgatatgag ccatcgtaga tatatatgtt cagcataagg aatcaccacc
     3181 attaatgtaa tccaataagg atagctgttc gtaattgctg aaaatgggta ttgctctttt
     3241 atccaggcta agatttgcgt ctatgaattt cgggtcaaac tttatatgcg gcatcatttt
     3301 ccctctgcag gttatgaaat actgggctct tttaagaaca acacccattt ttttgagttc
     3361 ctcaaacccc aaagaaccaa cttttcttgc agaaactatt tttcgtgcag atatggtgcc
     3421 gattcctgga acccttagca atgtgtcgta atccgccttg ttgacctcaa caggaaacaa
     3481 atccagattc cttacggccc aatcacattt cggatcatat ttaagattaa gattaggatt
     3541 tctttcgttt aaaagctcac tgctccgaaa cccataaaac cgcaatagcc agtccgcctg
     3601 ataaagtctg tgttctctta aaaggggagg ttctgccagt gaaggaaggc ttgggtcttt
     3661 tacagcaggt acataggccg agtagtatac gcgctttagc tgcatcttgt tgtacagatt
     3721 ttcggttaaa gtcagtatat cgtggtccgt atcgtctgtt gctccgatga tgagttgggt
     3781 gctttgacct ccaggaacaa aagtcctttt attgctgctg cctgttccat aacttgtggg
     3841 catggtttct cctaggggag caggcagatt gtctcttaga agaaatttgc cgcttttgtc
     3901 ttcttcgatt ctacctgaga tatatctcat tgagctgaag atgctcctct tctttttttg
     3961 gggtgcaagt cttgtcaggc ttgcttctga aggcagttct atgttgacgc tcattctgtc
     4021 ggcaagacgg ccagctctct ctatgagaag aggatcagct ccagggatgg ccttgacgtg
     4081 tatatatcca taaaaacaga actcctgcct caaaagtctc aaagtttcgt acattatctc
     4141 catggtgttg tcgggagaca gtattactgc agagcttagg aaaagacctt cgatatagtt
     4201 tctacggtag aattcgtatg tcagcatggc gatttccctg ggcataaatg ttgctctttc
     4261 cacatcattt gttcgcctgt taacgcagta tttgcaatcg tagacgcagt cgttgctttg
     4321 aagaaccttt aatagggaca cgcatcttcc atcctgggtg aagctgtggc atattccgca
     4381 ggctacactg tttcccatag cgccttgttt tgagcttcct cttcgctcaa caccactgga
     4441 agcacaggat gcatcgtatt ttgctgcagc gcccaatatg ttcagtttcc tt
//
LOCUS       DAONVY010000144         4485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13863_length_4485_cov_4.121896, whole genome shotgun sequence.
ACCESSION   DAONVY010000144 DAONVY010000000
VERSION     DAONVY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13863_length_4485_cov_4.121896"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1316)
                     /locus_tag="PLI11_05055"
     CDS             complement(156..1316)
                     /locus_tag="PLI11_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPZ52266.1"
                     /translation="MNRKRAPFSSHSFPVGPLGLIGMESCQDLAQKVDEWLIAMREEE
                     TQQKASSFLIKNRCPRFGNGEAKGIIDESIRGKDLYILIDVGNYGCTYNIMGRENVKS
                     PDDHFMDLKRIISAIGGKGKRINIIMPFLYGGRQHRRVIRESLDCALALHELQNLGIS
                     NIITFDAHDSRVQNSIPNISFENILPYYQFTKALLIAEPDIKIDKENLMIVSPDEGGI
                     HRNLYYSSIFGINLGIFIKRRDYSTMVNGRNPIVAHEFIGDDVEGKDILVVDDMISSG
                     DSLLDIAYELKRRKARKIYCAVTYALFTDGIDLFNKAYEDGALNRVFATNLTYRRPEL
                     KDAEWFVEVDMSKYIAKLIDALNYDESLGVLVNPAQRIISLLEKHSNGEPIV"
     gene            1549..2667
                     /locus_tag="PLI11_05060"
     CDS             1549..2667
                     /locus_tag="PLI11_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HPZ52267.1"
                     /translation="MDKKYDIAAYIWPAYTGDEPRTRMFWPEGMGEWQSVRDADKRFE
                     GHTWPRRPLWGYVNEADPYVMEMQIECAVDHGVNVFIYDWYWYDNRPFLENCLNDGFL
                     KAKNNHKMKFYLMWANHSVNNVWDKRLSHDLYNIIWDGAVSRSVFETIATRVIEKYFK
                     RSNYYTIDEKPVFMIYDIENLIRGLGGLEQTRNALDWFKNECIKRGLKGLHLQLTMWS
                     QNVKNVSGVDEERTMKPSEIIEVLGFDSMSHYQFVHFANIDRDYLEILKDVEKEWEAL
                     SKYKIPYFPHISVGWDNNPRFKEFRPGIVKNNTPENVEKAFRMAKEYADNHPNQPPLI
                     TINSWNEWTETSYLQPDDLYGYGYLEAIKKVFGNNAYF"
     gene            2741..4066
                     /locus_tag="PLI11_05065"
     CDS             2741..4066
                     /locus_tag="PLI11_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin oxidoreductase/NADH oxidase"
                     /protein_id="HPZ52268.1"
                     /translation="MQKFKYDKDSLMTYIKDFNLDLGYQDDISILKEEVDLGDGYISP
                     NRMAIHPLEGCDGHTDGSPSEFSLRRYKRFARGGAGLVWFEAIAICEESRANPRQLYI
                     TEQNKEVFKTFVDEIRKTRSEVSSKKQILIAQLTHSGRYSKPNGAASPICAYDNVELR
                     ESSGLPEDAFTYAADEYLDSLPDMFAKTALLLKEAGFDGVDIKACHGYLLGELLSAYE
                     REGKYGGSFENRVKLLMTSFKKVKEACGDNFILTVRLNLYDSYLKTENNPGWGGNVDK
                     MDLTEPIKLIGMLKDMGMKVINITMGNPYHNPHINRPYNAGGYTPPEHPLVGLGRLIE
                     GSAKVKQEYPDMIVVGTGYSWFRQCAPHVAANEIANNKTDIMGMGRVALAYPDFANDI
                     INHNRMFSDKICLTCSLCTKIMRAGRFAGCPVRDQEVYLPILREVRKYG"
     gene            4059..>4485
                     /locus_tag="PLI11_05070"
     CDS             4059..>4485
                     /locus_tag="PLI11_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPZ52269.1"
                     /translation="MARVAIVTGGTRGIGFGIVRLLVKNSFIVYAVGTREKEDDYPEF
                     NAFKKNNNNCKYICANIQNAKDRQKIVDTVMRNEKSIDLLVNNAGVAPKERNDILKMT
                     EESMDFVLGVNLKGTFFLTQLVANTMIEQVEKGKSSGIIV"
BASE COUNT         1427 a          873 c          945 g         1240 t
ORIGIN      
        1 gcctttgaaa tgatcaaaga cggtcttgtt tcctttgtgg gaggtttgga tattgccaaa
       61 gcttatcagg ttgtagagga aaacttcggt attgaagtgg ctgaaagcct tttcatcaca
      121 aacccacgta aaataatttt ggatgaatta atatactaaa caatgggttc tccgtttgaa
      181 tgcttctcca aaagacttat aatcctctgt gcaggattaa caagcacacc cagagactcg
      241 tcataattaa gtgcatcaat cagttttgca atatattttg acatgtcaac ctcaacaaac
      301 cattcagcat ctttaagctc tggccttctg tatgtcaggt tggttgcaaa tactctgttt
      361 aatgctccat cctcataagc cttgttgaac aagtctattc cgtcggtaaa cagtgcataa
      421 gtaactgcgc agtaaatctt tctagctttt cttctcttga gttcgtaagc aatatcaagc
      481 aatgaatctc ccgatgaaat catgtcgtcc acaaccaaaa tatccttgcc ttcaacatca
      541 tcgccaataa attcatgagc aactattgga ttcctgccgt taaccatcgt ggagtaatct
      601 cttctcttga tgaagatacc caggttgatt ccaaatattg atgaatagta tagattcctg
      661 tggattccgc cttcgtcagg gctcactatc atcaagttct ccttgtctat ctttatgtcg
      721 ggctccgcta taagcagggc tttggtgaac tggtaatatg ggagaatatt ctcaaagctg
      781 atatttggta tggaattttg gactcttgag tcatgagcat cgaatgtgat aatattgctt
      841 atgccaagat tctgaagttc atgcagtgcc agcgcacagt caagagattc tctaattacc
      901 ctcctgtgct gtcttccacc gtacagaaaa ggcataatta tatttatcct ctttcccttg
      961 cctccaattg cagagattat tcttttcaga tccataaagt gatcgtcggg cgacttcaca
     1021 ttttccctgc ccattatatt gtatgtgcag ccatagttac ccacgtctat caaaatatac
     1081 aggtccttgc ccctgattga ttcatcaatt ataccctttg cttctccgtt tccgaatctc
     1141 gggcatctgt tcttaattag aaacgaagat gctttttgtt gagtttcctc ctccctcatc
     1201 gcaattaacc attcgtcaac tttctgggcc agatcctggc agctctccat acctatcaat
     1261 cctaggggac ctacagggaa tgagtgcgat gaaaaaggtg ctcttttacg attcatttga
     1321 atactacctc caaaaatctg ctgttgatta ttacaataaa gaattcattt actacaaaaa
     1381 ccggacattt tcaaatactc cgctcaattc aattgtagca ttatattcat ctttattcaa
     1441 gcacaacata tggacagact gttattagat attgaaactg tccaccctgt tgtatataat
     1501 ctataattag tgtaaacatg ataaaatata caatatggag ctgataaaat ggataaaaag
     1561 tatgatatag ccgcatacat ctggccagca tatacaggcg atgaaccgag gacaagaatg
     1621 ttctggcctg aaggtatggg agaatggcag tccgtaaggg atgcagacaa gaggtttgaa
     1681 ggtcacacct ggccaagaag accactctgg ggttatgtaa acgaagcgga cccttatgtg
     1741 atggaaatgc agattgagtg cgcagtggac catggtgtca atgtattcat ctacgactgg
     1801 tattggtatg acaacagacc ttttttggaa aattgcctga atgacggctt tcttaaggca
     1861 aaaaacaatc ataaaatgaa gttctattta atgtgggcaa accacagtgt aaacaatgtg
     1921 tgggacaaaa ggctgtctca tgatctttac aacattatat gggacggagc tgtatcaaga
     1981 agcgtttttg aaacaatagc aacaagagtc atcgaaaaat acttcaagcg ttcaaactac
     2041 tatacaattg atgaaaaacc tgttttcatg atttacgaca tagaaaactt aataagaggc
     2101 cttggtggtc ttgagcaaac gcgaaatgct ttagactggt ttaaaaacga atgcataaaa
     2161 agagggttga agggtctgca tcttcaactt actatgtggt cccagaacgt aaagaatgtt
     2221 tcaggagtgg acgaagagag gacaatgaaa ccgtctgaaa taattgaagt gctggggttc
     2281 gacagcatga gccactatca gtttgtccat tttgccaata ttgacaggga ttatctcgag
     2341 attcttaaag atgttgaaaa agaatgggag gcactttcca agtacaaaat cccatatttc
     2401 cctcacatat ccgttggctg ggacaacaat cccaggttta aggaattcag accgggaata
     2461 gtaaaaaaca acacacccga aaatgttgag aaggccttta gaatggcaaa ggaatatgct
     2521 gacaatcacc caaaccagcc gccacttatt accattaaca gctggaatga atggactgaa
     2581 accagctacc tgcagccgga tgatttgtat ggatatggat atttggaggc tataaaaaag
     2641 gtatttggca ataatgcata tttttaaaat gataattctt atttatacac caactgttgt
     2701 ggtagtattt aagggaatta gaattttagg aggattttaa ttgcagaaat ttaaatacga
     2761 caaagacagc cttatgactt atataaaaga tttcaacctg gatttgggat atcaggatga
     2821 catttcaata ctcaaggaag aagttgattt gggagatggt tatatctccc caaaccgcat
     2881 ggcaatacac cctttggagg gctgcgacgg ccataccgac ggttcaccgt cggagttttc
     2941 cttgagaagg tataaaagat ttgcacgagg cggagcagga cttgtttggt ttgaagctat
     3001 tgcaatctgt gaagagtcgc gggcaaaccc aagacagtta tacataaccg aacaaaacaa
     3061 ggaagtgttt aagacatttg tagatgaaat aaggaagaca cgcagtgaag tcagcagcaa
     3121 aaaacagata ttgatagcac agcttacaca ctcaggaaga tactcaaaac caaatggagc
     3181 tgcttccccc atatgcgctt atgacaatgt ggaattaagg gaaagctccg gcctgcctga
     3241 agatgccttt acatatgcag ccgatgaata ccttgattcc ctgccagata tgtttgcaaa
     3301 gacagccctt ttactaaagg aggcaggctt tgatggtgtg gatattaaag catgtcacgg
     3361 ctatctcctg ggggaacttc tttctgctta cgaaagagaa ggcaaatacg gaggttcttt
     3421 tgaaaacaga gtaaaactac ttatgacctc ttttaagaag gtaaaggaag cctgcggcga
     3481 caactttata ttaactgtaa gacttaatct ttacgattcg tacctaaaga ctgaaaacaa
     3541 tcccggctgg ggtggcaatg tcgacaagat ggatttgacc gaacctatca aactaattgg
     3601 gatgcttaaa gatatgggaa tgaaagttat caacattaca atgggtaacc cctaccacaa
     3661 tccccatatc aacaggccct acaatgcagg aggatacacc ccacctgaac atccactggt
     3721 tggccttggc agacttatcg aaggttcggc taaagtaaaa caggagtatc ctgatatgat
     3781 tgttgtgggt accggctaca gctggttcag gcagtgcgcc cctcatgttg ctgccaatga
     3841 aattgcaaac aacaaaacgg atatcatggg catgggaaga gttgcccttg cgtacccgga
     3901 ctttgcaaat gacataataa atcacaacag aatgttttca gataaaatat gccttacatg
     3961 cagtctgtgt acaaaaatca tgcgtgcagg aagatttgcc ggctgtcctg taagagacca
     4021 ggaagtatat cttccaatat tgagggaggt tagaaaatat ggctagggtg gctatagtaa
     4081 ctggaggaac ccgagggata gggtttggca tagttagatt acttgttaaa aactctttta
     4141 ttgtctatgc cgtgggaact cgtgaaaagg aagacgacta tcctgaattt aacgccttca
     4201 aaaagaataa taataactgt aaatacatat gcgcaaacat tcagaatgca aaagacaggc
     4261 aaaagattgt cgacacagtc atgcggaatg agaaaagcat tgatttgctt gtaaacaatg
     4321 caggtgttgc accgaaagaa agaaacgaca tattgaaaat gactgaagaa agcatggact
     4381 ttgtattggg tgtaaactta aaaggcactt tctttcttac acagctggtg gcaaacacaa
     4441 tgatagaaca agtggaaaaa ggcaaatcat ccggcatcat tgtaa
//
LOCUS       DAONVY010000145         4484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13871_length_4484_cov_3.731091, whole genome shotgun sequence.
ACCESSION   DAONVY010000145 DAONVY010000000
VERSION     DAONVY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13871_length_4484_cov_3.731091"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLI11_05075"
     CDS             <1..586
                     /locus_tag="PLI11_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPZ52270.1"
                     /translation="IMGPAVSCECLEQKGMHIQEDHFLIEIIDPTTGNHVPEGEKGEV
                     VFTTLTKEGFPLIRYRTRDVSCIDTSQCACGRTTARMMKLCGRTDDMLIIRGVNVFPS
                     QIESVLLELGETAPHYLIIVDRVGNHDTIEVQVEMTENFFSDSVKYIESLEHRIKSKL
                     DSVLGLNVKVSLVEPKTLARSEGKAKRVIDKRKM"
     gene            599..1030
                     /locus_tag="PLI11_05080"
     CDS             599..1030
                     /locus_tag="PLI11_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPZ52271.1"
                     /translation="MGVRQISVFLENKAGRLSDVTKVLGDNNVDISALSIADTAHFGI
                     LRLIVNNPDLAETVLKKNGYTVSISNVIAIAIDDNPGALAKALEVLTDEDITIEYIYA
                     FTGKTENKALVVLKVEDIEKATELLRSRNIRVLDDSEVYSL"
     gene            complement(993..1922)
                     /locus_tag="PLI11_05085"
     CDS             complement(993..1922)
                     /locus_tag="PLI11_05085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPZ52272.1"
                     /translation="MQPTDRLTYVVSEKDEGKKISRILKDRTKLSSGMIRVVKYNEGV
                     LLNGENVRVNHLVKCGDVITVLLNCRQEDTDIIPTNIPLEILYEDDAMLVCNKPPYMV
                     SHPVRNHLADTLLNAVVYRMNEEGKRHGIHLVSRLDKDTSGIVIIAKNPYVQEQLKLQ
                     GERNVFIKKYLGLTNGQPNYDSGIIDAPIARKEEYSMERIVHEEGYASVTEYKVIERY
                     ENHALVEFRPITGRTHQIRVHCLYENFPLLGDSLYNKPCSFMERQALHAYKVYFIHPV
                     INMPMEIHAPLPKDMADALNIIKGCKPRNHQEP"
     gene            2139..3473
                     /locus_tag="PLI11_05090"
     CDS             2139..3473
                     /locus_tag="PLI11_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52273.1"
                     /translation="MKRKLICLILVTILLCSLFSMNVMGKTVLFSNEDGVTIFYEDFE
                     DYEMPADLINNLMGYWRFEDDSALGSGHNEFNIIETEDGTQALYAHWIHGRLMKSDTD
                     LIVKSPFVLGGRIMAQGDTATNHGTFIWIRSSKTPLVSQNGRLLNEYEEDIDKEVGGT
                     GLALELYSNKIDVIIREWSEETEDGLFSNRTPIDLPEGVDLTNNFVDFLVEDDGNVIK
                     VTFDNTLLCTIEYSEPKEGYYSKAVVKDAEGNVLNESEVATLCADDEAAIAFANRAGI
                     FTIDEVYYIGNAAAGKNVYANKKTYTTEDDIEIGYLGAEENYWIGVYKKGTTPSNENQ
                     PIKRVDVVGMGAELLKAVDLTLEPGEYDIVLFENNEYKEVSKITVTVEEPPTPEPTDE
                     PADETPAPSSPSDEDEDDKEGGNTLVYILVAVGVVVIAAVVIALSVIKKKKK"
     gene            3578..>4484
                     /locus_tag="PLI11_05095"
     CDS             3578..>4484
                     /locus_tag="PLI11_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HPZ52274.1"
                     /translation="MLKNLLRNEYGINDDLFRLSQEIEDQIKEKFKEIDNIKEYNQLK
                     VLSAMQKMGLSDRHLLGTTGYGYNDIGREIIEEIYSEVFKTEDALVRNQIVSGTHALA
                     ICLFGILRPGDTMLSCAGKPYDTLESVIGTKPTPGSLLEFGVKYEQVDLLPNDEPDLE
                     KIFEAVNENTKLALIQRSRGYSYRKALSIESIRDIIKTVKSKNKNTICMVDNCYGEFV
                     CCKEPSEVGADIIAGSLIKNPGGGLALNGGGYVAGKESIVKLCANRLTAPGLGKRVGA
                     SIGAIRNIIQGFFLAPHVVSECLKGA"
BASE COUNT         1387 a          790 c         1021 g         1286 t
ORIGIN      
        1 aatcatgggg cctgcagtat cctgtgaatg ccttgagcaa aagggaatgc acatacagga
       61 agaccatttc ctgattgaga ttatagatcc cactaccgga aatcatgtac ctgagggtga
      121 aaagggtgaa gttgtattta caactctcac aaaagaaggc ttcccactaa tcagatacag
      181 aacaagggac gtatcatgta ttgacacaag ccagtgtgcc tgcggaagaa ccactgccag
      241 aatgatgaaa ctgtgcggta gaactgatga tatgctgata attagaggtg taaatgtatt
      301 cccatcacaa attgaaagtg tgcttctgga acttggagaa acagctcctc attatttaat
      361 aattgttgac agagtaggca atcatgatac aatagaagta caggtagaga tgactgaaaa
      421 ctttttctcc gactcggtta agtacattga gagtttggag catagaatca agtcaaagct
      481 tgattctgtt cttgggctta atgttaaagt aagtctcgtt gagcctaaaa ccttggccag
      541 aagcgaaggg aaagccaaga gagtcattga taagagaaag atgtaagggg gcttggttat
      601 gggggtaaga cagatttccg tattcttgga gaacaaggca ggcaggctgt ccgatgttac
      661 caaagttttg ggagacaata acgttgacat atcggctttg tccattgctg atactgctca
      721 ttttggaatt ctacgactta tcgtcaacaa tcctgatttg gcagagacgg tcttgaagaa
      781 aaatgggtat acagtcagta tttcaaacgt aattgccatt gcgatagacg ataatcccgg
      841 tgctttggca aaagcgctcg aagtattaac agacgaggac atcacaatag agtatatcta
      901 tgcctttaca ggcaagacgg agaacaaggc attggttgtt cttaaagtag aggatataga
      961 gaaggcgaca gagctcctca gatcaagaaa catcagggtt cttgatgatt ccgaggttta
     1021 cagcctttaa ttatatttaa tgcgtctgcc atgtcttttg gcagaggtgc atgaatttcc
     1081 atgggcatgt taatgacagg gtgaatgaaa tacaccttgt aggcatgaag tgcttgtctc
     1141 tccataaatg aacaaggctt gttatacaag ctatcaccaa gcagaggaaa gttttcatac
     1201 aggcagtgca cacgaatctg gtgtgttctg cctgttattg gtctaaactc aacaagcgca
     1261 tggttttcat atctttcaat taccttgtat tctgtaactg aagcatagcc ttcttcatga
     1321 actattcttt ccattgaata ctcttctttt cttgcaattg gcgcatctat tattccagaa
     1381 tcataattgg gttgtccatt cgttaatccc aaatactttt taataaatac atttctttcg
     1441 ccttgaagct tcaattgctc ctgaacatag gggttttttg caataattac tatgcctgaa
     1501 gtatccttgt caaggcggct taccaagtgt attccgtgtc tttttccttc ttcattcatc
     1561 ctgtacacca ctgcgtttaa caaagtgtcg gcaagatgat tccttacagg gtgggatacc
     1621 atgtaaggtg gtttgttgca tacaagcatt gcatcatctt catacaggat ttcaagggga
     1681 atgtttgtcg gaattatgtc tgtatcttcc tgtctgcagt ttaacaacac tgtgattaca
     1741 tctccgcatt taaccaaatg attaacacgt acattttcac cattcaacaa aaccccttcg
     1801 ttatacttca caacccgtat cattccggaa gaaagcttcg ttctatcctt caatattctg
     1861 ctgattttct ttccttcatc cttttcactt accacatatg tcagtctgtc tgttggctgc
     1921 attttatcct ctttcaaaac tatattctat tcatttttat tatagcatat tttgttaatg
     1981 ttaacagata ggtctctacg gccttgttga gccaaatggt tctgacaaaa ctctcctttt
     2041 attatactgc ttaaagattt caaaatatta tgttgaaaga tgtattttaa taatgttata
     2101 ataatgttaa caaaaaaact gttaggaggc gtacaggtat gaaaagaaaa cttatttgct
     2161 taatacttgt aacgattttg ttgtgttcgt tattttcaat gaatgtaatg ggaaagacgg
     2221 tcctgttttc aaatgaagat ggcgtaacca tcttctacga ggattttgaa gattatgaga
     2281 tgcctgctga cttgattaac aatctcatgg gctactggag gtttgaagac gacagcgcac
     2341 tgggttccgg acataatgag ttcaacataa ttgaaactga agatggaacc caggcgttgt
     2401 acgctcactg gattcatggt cgacttatga aaagtgatac agacctgatt gtaaagagtc
     2461 cgtttgtatt aggtggaaga ataatggcgc agggagacac tgctactaac catggaacct
     2521 ttatttggat aagaagctcc aaaacacctt tggtttcgca aaatggcagg cttctcaatg
     2581 agtatgaaga agacattgac aaggaagttg gaggcaccgg acttgctctg gaactatata
     2641 gcaacaagat agatgtcata attagagagt ggagcgagga gacagaagac ggactgtttt
     2701 ccaacaggac accaatagat cttcctgaag gagtagatct aacaaacaat tttgttgact
     2761 ttcttgttga agatgatgga aatgttataa aagtaacatt tgacaacaca ttattgtgca
     2821 ctattgaata ttccgaaccc aaggaagggt actacagtaa ggcggttgta aaggacgcag
     2881 agggcaatgt gctgaatgaa tccgaggttg caaccttgtg tgctgatgat gaggctgcga
     2941 ttgcctttgc aaaccgtgca ggcattttta ctattgatga agtatactac attggaaacg
     3001 ctgcggcagg aaagaatgtt tacgcaaata aaaagactta tacaaccgag gatgacatag
     3061 agattggata tcttggagct gaagagaact actggattgg tgtatacaag aagggaacaa
     3121 caccaagcaa tgagaatcag ccaataaaga gagtagacgt ggttggtatg ggagccgagc
     3181 tacttaaagc ggttgatttg acgcttgaac cgggtgaata tgatatcgta ttgtttgaaa
     3241 acaacgagta taaagaagtt tccaagataa ccgtcacagt agaagaacca ccaactcccg
     3301 agcccaccga tgagcctgct gatgaaactc ctgcacctag cagtccttcc gatgaagatg
     3361 aggatgacaa agagggtggc aatactctgg tatatatcct tgttgcagta ggtgttgttg
     3421 ttatagcggc tgttgtgatt gctttgtcgg taattaagaa aaaaaagaaa taatggatga
     3481 tacagtggat gtctaaatgg catccattgt ttgattaata gcaagtaatg tgataaaatt
     3541 attcctcgtt atatttatca tgaattggat gggtgttatg ttgaaaaacc ttttgaggaa
     3601 tgaatatgga ataaacgatg atctttttcg tttaagtcag gagattgaag accagattaa
     3661 agaaaaattc aaagaaatcg ataatattaa agagtacaac caactaaaag ttctttcagc
     3721 aatgcagaaa atgggattgt cagacaggca tttgcttgga accacaggtt atgggtacaa
     3781 cgatattgga agagaaataa ttgaggaaat atattctgaa gtatttaaga ctgaggatgc
     3841 attggttaga aaccagattg tatcaggaac ccatgccttg gccatatgtt tgtttggaat
     3901 actaagacct ggcgatacga tgcttagttg cgcagggaaa ccctacgaca cactggaatc
     3961 agttattggt acaaaaccaa caccaggttc cttgcttgaa tttggagtca agtatgagca
     4021 ggtggatctt ttgccaaatg acgagccgga tttggaaaag atatttgagg cggtaaatga
     4081 gaacaccaaa cttgcactga tacagcgttc aagagggtac agctacagaa aggctctttc
     4141 tattgaaagc ataagggata tcatcaagac ggtgaagtca aagaacaaga acaccatatg
     4201 catggtggac aactgttacg gggagtttgt atgctgcaag gaacccagtg aagtaggtgc
     4261 tgatattatt gccggctccc ttattaaaaa tccaggtgga ggacttgcct tgaatggcgg
     4321 tggatatgta gcaggcaagg agagtattgt taagctgtgt gccaacaggc ttacagcacc
     4381 cggacttgga aaacgtgtag gagcatccat aggcgccata aggaacatta tccaagggtt
     4441 ctttcttgcg cctcacgtgg tatcggaatg tctcaaagga gcgg
//
LOCUS       DAONVY010000146         4480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13885_length_4480_cov_3.839548, whole genome shotgun sequence.
ACCESSION   DAONVY010000146 DAONVY010000000
VERSION     DAONVY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13885_length_4480_cov_3.839548"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1629
                     /locus_tag="PLI11_05100"
     CDS             184..1629
                     /locus_tag="PLI11_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPZ52275.1"
                     /translation="MRKSFVLPKSLVKDSEVKAKEKWLGYLLGPAGALLLNAVLATYL
                     NVYYTDVLGLTKLWGGAFLVIFPIISKIIDAITNVIMGYIIDRTHTKEGKARPWLLVS
                     APLLTIAGILLFTVPKASTTVQAIWVIVSYNLFYSFAYTIFNMSHNLMVPLSTRDTKQ
                     RGGLSVFNQITTIMMSGILVALIFPMVIMPMIGVDKSKWILLMSLLSILALPLTLLEY
                     FFTKERVTLEKAEFKNEVKIPYLKQLKAIFTDKYMIIIYLYFLIYTMGSSIKNLSLVY
                     FCNYVLGTYNDGITQTMISVIGGIPMGIGIFAVWPLAKRFGKRNVTLAGFILYSIGGI
                     ICWIFPTNMVIMLIGQFIKNIGGLPCSYVFMALFADVLDNFEWKVGFRSDGLAMSVYN
                     IIAVAMVGICTGIFNGLLSAAGYVQPYYDEAGNLIATQTEAVKSAITFGFVGLEVFTG
                     IILAILLIFLNVEKGLEKKQAEIKARQEAKE"
     gene            1626..4181
                     /locus_tag="PLI11_05105"
     CDS             1626..4181
                     /locus_tag="PLI11_05105"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPZ52276.1"
                     /translation="MKAVRIKINHLFSPIGITDSKPTISWNLSDGIKQTAYRIQAYDE
                     KSNILWDSGKVDSEHLHAKWLGEPVLPHTRVRFTLTIWDENGNEGKISESFFETGLPV
                     GEEWQARWISGNYRVRKSKRYPVDCFKKVFSANNIVKARLYITACGLYATRINGKRCG
                     SFVLSPGITDYRRRVQYQTYDVTDLVKDGENVIEVELADGWYRGSCGAWGRRNQYGTQ
                     TKLLAQLELKKADGSTEIIGTDDTWSWSNDGPIRFADNKDGERVDARRVPSYSGYAKE
                     ARHNVIPTPSDNVPVTEHERFKAKIIKTPLGKTVLDFGQNMAGYVEFSVNAKAGQKIL
                     LRYGEYIDKDGEFTQKNFQLSIRNYTTPLQQTEYICRGGENRYKPKFSIFGFRYVQIE
                     SDIEINPEDFTAIAVYSDLETTGTFSCSNTLINKFFENTVWSAKSNMCDLPTDCPTRE
                     RHGWSGDAQIFCSTACYLFDYYTFAVKYLNDLYDWQKKNGCLPQIAPEGGTDFYMRAM
                     NGSVGWADAGIIIPYVLWKQYNDRKIIKKYLSGMEKYAAFQMKRCGKWYPTAKRTGLC
                     GKDKRYLNNAGQSYGEWAEPDDVHHMTWKDCVVPHPEVATAYTSYVLSLLAEMEEELD
                     RADKAEEYRQFSNNCKESYRALVKTDKYLLDTDRQARLVRPLALDMLDSEQKKYAEQR
                     LIKALDNYDWRVGTGFLSTPFILDVLKDINPEHAYRLLENEEMPGWLFMPKNGATTVW
                     EAWKGPESKKGGIGSLNHYSKGAVCRFLFDTVCGIKVSGDNEFIVSPLPGGSLTYAKA
                     SYKSVYGEITSSWERKEGKTVYTVTVPPGCRAMVKLPDGKEYIQTAGTWEYEE"
     gene            4183..>4480
                     /locus_tag="PLI11_05110"
     CDS             4183..>4480
                     /locus_tag="PLI11_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52277.1"
                     /translation="MSKIKEIISRMTLEDKVAFCSGADFWHTKAMSQYEIPEMMLSDG
                     PSGLRKQESASAQDMLGINKSVPATCFPSASTTASSFDPAVTESIGCAIGVEALA"
BASE COUNT         1355 a          867 c         1061 g         1197 t
ORIGIN      
        1 ggacaggcaa agcagataac gaagccaggg attttgccaa atactcatag tctttaagca
       61 tgctgccacc tcatttttta ctacaatata taataaaaca gacaataatg caataaatca
      121 ggagggtgat aaggtataat aaaatgcatg attgacttag tgtatattgc ggagggttaa
      181 tatatgagaa aatcctttgt tttgccgaaa tctcttgtta aggattctga agtaaaagca
      241 aaagaaaaat ggctagggta tcttctgggg cctgccggtg cattgcttct caacgcagtt
      301 cttgcaacct atcttaatgt atattatacc gatgtgctcg ggctgaccaa gctgtgggga
      361 ggagcgttcc ttgttatctt tccgattatt tcaaaaataa tcgatgccat caccaatgtt
      421 ataatgggat atatcattga ccgtactcat accaaagaag gcaaagcgcg gccatggctt
      481 ttagtatccg caccactttt gacaattgcg ggtatcctgt tgtttacagt acctaaggca
      541 tcgacaacgg ttcaggcaat ttgggtaatc gtatcataca acctctttta ttcctttgcc
      601 tatacgatct tcaacatgag ccataatctt atggttcctc tttctacacg ggatacaaaa
      661 cagcggggag ggttgtcagt tttcaatcag attaccacga taatgatgag cggcattctc
      721 gtggctctca ttttcccgat ggtaattatg ccgatgattg gcgttgacaa gtcaaaatgg
      781 attcttttga tgtcactgct ttcaattttg gccctgccgc taaccttgtt ggaatatttc
      841 tttacgaaag aacgtgtcac cctggaaaaa gctgaattca aaaacgaggt taagataccg
      901 tatttaaaac agctaaaagc aatttttaca gataaataca tgattatcat ctatctgtac
      961 tttctcattt acaccatggg atcgtcaatt aagaatctca gtcttgtata tttctgcaat
     1021 tatgtgctcg gtacatacaa tgacggcatc acacagacaa tgatatcagt tatcggcggt
     1081 attccgatgg gaataggcat tttcgcagta tggcctctgg caaagagatt tggaaagcga
     1141 aacgttactc tggctggatt cattctatac tctataggag gcattatctg ctggattttc
     1201 ccgacaaata tggtcattat gctgataggg cagtttatca agaacattgg aggtcttcct
     1261 tgctcatatg tctttatggc tttatttgcc gacgtacttg acaacttcga gtggaaggtt
     1321 ggttttcgtt cagatggttt ggccatgtct gtgtataata taattgcagt agccatggtt
     1381 ggaatttgca cgggtatatt taatgggctt ctttcggcag caggatatgt tcaaccatat
     1441 tatgacgaag caggcaatct gattgcaacc cagacagaag ccgtcaaaag cgccattact
     1501 tttggttttg taggtcttga agtgtttaca ggaattatac ttgccatact tctgatcttt
     1561 ttgaatgttg agaaaggtct ggagaagaaa caggccgaaa tcaaagcaag acaggaggca
     1621 aaagaatgaa agctgtaagg attaaaataa atcatttgtt ttcacctatt gggataaccg
     1681 attccaaacc tacaatttcg tggaatcttt ctgatggtat aaagcagact gcgtacagga
     1741 ttcaggcata tgacgaaaaa tcaaacatac tttgggacag tgggaaggta gatagtgaac
     1801 atcttcacgc caaatggctt ggagaaccag ttctgccgca tacacgggtg agatttacac
     1861 tgacaatatg ggatgaaaac ggcaatgaag ggaagatttc tgaatcgttt tttgaaacag
     1921 gcttgcccgt aggcgaagag tggcaggcca ggtggatttc tggcaactat cgtgttcgga
     1981 aatcaaaacg ctatccggtt gactgtttta aaaaggtttt ctcagccaac aatattgtaa
     2041 aggccagatt gtacattact gcatgcgggc tatacgcaac aagaatcaac ggcaaaaggt
     2101 gcggtagttt tgtcctttcg ccaggaatta ccgactacag gcgcagggtt cagtatcaga
     2161 catatgatgt gacagacctt gtcaaagatg gtgaaaacgt gatagaggtg gaactggcag
     2221 atggttggta tcgaggttct tgcggtgcct ggggtagaag aaaccagtac ggcacacaga
     2281 caaaactact ggctcagctt gagctaaaaa aagctgatgg cagtacagag attatcggca
     2341 cggatgatac ctggagttgg agtaatgatg gtccaatacg ttttgcagac aacaaggacg
     2401 gggaaagggt agacgcaagg agggtgccgt cctattcggg ttatgcaaaa gaagcaaggc
     2461 ataatgtaat tcctaccccc tccgacaatg tgcctgtgac agagcacgag cgcttcaagg
     2521 caaagattat aaaaactccg cttggcaaaa ccgttcttga ttttggtcag aatatggcag
     2581 gatatgtgga gttctccgta aatgccaaag ccggacagaa gattctgctg cgctatggtg
     2641 aatatattga caaggacgga gagtttacac aaaagaactt tcaactgtca ataaggaact
     2701 atacaacacc tcttcagcag actgaataca tctgccgggg tggagaaaac aggtataaac
     2761 ccaagttttc aatatttggc ttccgctatg tgcagataga gagcgatatt gaaataaatc
     2821 cagaggattt tactgcaatt gcagtttatt ccgatttgga gacaacagga accttttctt
     2881 gctcaaacac actaattaac aaattctttg agaatactgt atggagtgca aaaagcaata
     2941 tgtgcgatct gccaacagac tgcccgacaa gagagcggca tggatggtca ggagatgcac
     3001 agatattctg ctcaactgcc tgttatctgt ttgattacta tacctttgcg gtcaagtatt
     3061 tgaatgactt gtacgactgg cagaagaaaa acggatgcct tccccaaatt gcacccgaag
     3121 gcggtacgga tttttacatg cgtgcaatga acggttctgt gggatgggct gatgcaggca
     3181 taataattcc ctacgttcta tggaaacaat acaacgacag aaaaatcatt aaaaaatatt
     3241 tatccggcat ggaaaagtat gctgcatttc agatgaaaag atgcggcaaa tggtatccaa
     3301 cggcaaagcg gacgggactg tgtggaaaag acaaaaggta ccttaataat gcagggcaga
     3361 gctatggtga atgggcagaa cctgacgatg tacaccatat gacgtggaag gattgcgtgg
     3421 ttccacatcc tgaagttgcc accgcatata cctcatacgt tctttctctt cttgcagaaa
     3481 tggaagaaga acttgatcgg gctgataaag ctgaagaata cagacaattt agtaataact
     3541 gcaaagagtc ctaccgtgcg cttgtgaaaa ctgacaagta cttgctcgat acggacagac
     3601 aggcacggct tgttcgtccg cttgcccttg acatgcttga cagtgaacag aaaaagtatg
     3661 cagagcaaag attgataaaa gctcttgata attacgattg gcgtgtcgga accggctttt
     3721 tgtcaacgcc cttcatactt gacgtactaa aggacattaa tcctgagcac gcttacagac
     3781 ttcttgaaaa tgaagaaatg cccggctggc ttttcatgcc gaaaaatggc gcaaccacgg
     3841 tctgggaagc atggaaagga ccagaatcaa aaaagggcgg tataggttct ctcaatcact
     3901 attcaaaggg agcggtgtgc cgtttcttgt ttgacactgt ctgcggaatc aaagtttccg
     3961 gcgataatga atttattgtt tcacctttgc ctggaggcag tctgacatat gcaaaagctt
     4021 cgtataaaag cgtttacgga gaaattacaa gctcctggga gagaaaagaa ggtaaaactg
     4081 tctatactgt aacggttcct ccaggctgcc gtgcaatggt aaaattgcca gacgggaaag
     4141 aatacattca aactgcgggg acgtgggaat atgaggaatg atatgagtaa gataaaagaa
     4201 attatttctc ggatgacatt ggaggacaaa gtggcttttt gctcaggtgc ggatttctgg
     4261 catacaaaag caatgtcgca gtacgaaatt cccgaaatga tgctctccga cggcccgagc
     4321 ggactaagaa aacaggaaag tgcaagcgct caggacatgc tcggaataaa caaatccgta
     4381 cctgcgacct gttttccttc tgcatctaca acagcatcaa gctttgaccc tgccgtgaca
     4441 gaaagcatcg ggtgtgctat tggtgtagag gctttggcac
//
LOCUS       DAONVY010000147         4464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13938_length_4464_cov_3.235654, whole genome shotgun sequence.
ACCESSION   DAONVY010000147 DAONVY010000000
VERSION     DAONVY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13938_length_4464_cov_3.235654"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="PLI11_05115"
     CDS             <1..1539
                     /locus_tag="PLI11_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52278.1"
                     /translation="IYFSYPLVGHMYASIVISNANGENITFDGRNIYLEGGGRIAFVA
                     GGHYPDTVEETKKYFELNVEDEKKKARDRYAEYFNNLPDFESMIPLDMPMRDTLLKAI
                     ESNVINIKTQQHEEGGVIAGYPYHLGYVRDQYGVVRGMLKLGMKEEALGVLYFFLNEF
                     KREGRINNAHGMGLRGVMHIHENDDVEITGYIVLMFFDYLGFVEDEGERRRLFDEFLP
                     LLKWALEAQISQVVNGMLPFNGDETYIAGGILPRNAIFDGSSEATLMFIDSALKFMNE
                     VKRLGIDFDVSHIKEKVDECRKLYARNFFVDGKLITNNPDRITDDEKPMYRHGVCEGY
                     CGYFGWIEKSKEALYLCVDCLEKEVLGEREFRNHKKVHKLKSVLLLPIFINSTVITIN
                     DIMNDIYEISLKYLETGRIPSASGMDRNLGYDFGFLLGSLNEVMKVIDKEDERFDVFL
                     RAIMRIYEDTLNLVDRTYTWCEYYNDLKSSGTQYRPWESAINIKALIEFAENFNIIKE
                     YIEK"
     gene            1536..2411
                     /locus_tag="PLI11_05120"
     CDS             1536..2411
                     /locus_tag="PLI11_05120"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPZ52279.1"
                     /translation="MNQVGGTYIFTPVNNIKGVVHVIHGMAEHFDRYREFASFLNDHG
                     YVVCGTDLPGHGRSVGEKKGYFKSHEDVVKAVLELNKSIREMYNGMRIICFGHSMGSF
                     FAEYIASKHKGEFDAYVFSGTQGVNGIIPLAKFIAGIEVLFRGEKHESKLLSRLSFGM
                     YNKAFKPTRTDFDWLTRDHERVVDYVKDDLCGFTFTSKAYFEMFKVLKEVNSSKWAKS
                     LDKDLKYLFVSGDRDPVSNNGKGVLKLVERMKKAKVKDVRVKLYEDCRHELLNELNRE
                     MVFKDILDFIEEAVS"
     gene            2408..3883
                     /gene="glpK"
                     /locus_tag="PLI11_05125"
     CDS             2408..3883
                     /gene="glpK"
                     /locus_tag="PLI11_05125"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPZ52280.1"
                     /translation="MKKYVISLDQGTTSSKALLFDRDLNLIHKKQKELTQIYPNAGWV
                     EHDPKEVYESSFSVINDVIKEAGISAAEICAIGITNQRETAIVWDRYTGEPVYNAIVW
                     QCRRSASMCDEIKRRKELFGYIKENTGLIVDAYFSATKVKWILDNVEGAYDRAKNGDL
                     LFGTVDSWLLYNFTKKHMTDSTNASRTMLFNIRDMKWDERLLSEFDIPKSMLPEVCPS
                     GHVFGETTCFGHTIPIAALSGDQQSSLFGQNCFEKGDMKNTYGTGCFLLVNTGDEFIQ
                     SDSGMITTLASDVGVMRKYAVEGSVFTGGSVIQWLRDELGILESSAQSEEFALKVKDS
                     LGVVFVPAFTGLGAPYWNMDVRGSMFGITRGTNKYHIARAALESIAFQCRDVIEVIKQ
                     DAKVDISSLKVDGGATSNNFLMQFQADITNMEIVRPKMTESTGFGAALLAGLTVGFFE
                     SKDEIRQKYKVDRKFVPEMTKDERRKIIDRWKWAVGLVTQK"
     gene            4043..>4464
                     /locus_tag="PLI11_05130"
     CDS             4043..>4464
                     /locus_tag="PLI11_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52281.1"
                     /translation="MKTISILSAKERFLSKFPCNILKEYPVDIEIAREDKLLERIEGI
                     FERNGRDLFTLMSLNEQGELSNATTLIYQDKAYVLIKDVPKLTDFYVTGAVYTEMARI
                     YVTRSCCVKRSDILQFSFNANCLIGYGFWIEYATKFIA"
BASE COUNT         1382 a          727 c         1138 g         1217 t
ORIGIN      
        1 atctactttt catacccttt ggttggacac atgtacgcat ccatcgtgat ttcgaatgca
       61 aatggagaaa acattacttt tgatggaagg aatatttatc ttgagggcgg cggaaggatc
      121 gcctttgtag ccggtgggca ttatccagat acggtagagg aaacgaaaaa gtattttgaa
      181 ttgaatgtgg aagatgaaaa gaaaaaggca agagatagat acgcagagta ttttaacaac
      241 cttcccgatt ttgaatccat gattcctctg gatatgccga tgagggatac actcctaaag
      301 gcgatagaat ccaacgtgat taatattaag acacagcagc atgaggaagg cggagttatt
      361 gcaggatatc cttatcatct gggttatgta agagaccagt atggtgttgt aagggggatg
      421 cttaagctgg gcatgaagga agaagctctt ggtgttttgt atttcttcct caatgagttc
      481 aagcgagagg gaaggataaa caacgctcac ggcatgggtc tgaggggtgt catgcacata
      541 catgaaaatg atgatgtaga gataaccgga tatatagtcc ttatgttctt tgactacctg
      601 ggtttcgtgg aggacgaggg cgagaggaga aggctttttg acgagttttt gccacttttg
      661 aagtgggctc tggaggcaca aatcagtcag gtagtaaacg gcatgctgcc attcaatggt
      721 gatgaaacat acattgctgg cggaatactg ccaaggaatg cgattttcga cggatcctcc
      781 gaagcaacac tcatgtttat tgattcggct ctcaaattca tgaatgaagt taaacgattg
      841 ggaattgact ttgatgtaag ccacataaag gagaaagtgg atgagtgtag gaagctttat
      901 gcaaggaatt tctttgttga cggaaaactc ataaccaaca atcccgacag aataacggac
      961 gatgaaaagc caatgtacag acatggtgtt tgtgaaggct actgtggata ttttggctgg
     1021 attgaaaaga gcaaggaagc gctttatctg tgtgtggatt gtcttgaaaa ggaagtcctt
     1081 ggagaaaggg agttcaggaa ccacaagaaa gtgcataagc tgaaaagtgt tctgcttctt
     1141 ccgattttta tcaattcaac cgttatcacc attaatgaca taatgaatga tatttatgag
     1201 atatctttga aatatcttga aacgggtaga ataccctctg catcaggcat ggacagaaac
     1261 ctaggatatg actttggttt ccttcttggc agtttgaacg aggttatgaa agttatcgac
     1321 aaggaggatg aaaggtttga tgtgttctta agggcaataa tgcgcattta tgaggatact
     1381 ctcaatctgg ttgacagaac atacacctgg tgcgaatact acaacgacct caagagttcc
     1441 ggaacccagt acagaccctg ggagagtgcc ataaacataa aagccttgat tgagtttgct
     1501 gaaaatttca atatcataaa ggagtatatt gaaaaatgaa tcaagtaggc ggaacatata
     1561 tctttactcc tgtgaataat atcaaaggtg tcgtgcatgt catacacggc atggcggaac
     1621 attttgatcg atacagagag tttgcgtcat ttttgaatga tcatgggtat gtggtttgcg
     1681 gtacagacct gcctggacac ggcaggtctg tgggtgaaaa aaaggggtat ttcaaaagcc
     1741 atgaggatgt tgtgaaggct gttttggagt taaacaagag tattagggaa atgtataatg
     1801 gcatgaggat catttgcttt ggacattcca tgggttcgtt ttttgcagaa tacatcgcat
     1861 caaaacacaa aggtgaattc gatgcatatg tttttagcgg aacccagggg gtaaatggaa
     1921 ttattcctct tgccaaattt attgctggta ttgaagtttt gtttagaggc gaaaaacatg
     1981 agagcaaact gctgtctcgt ttaagtttcg gcatgtacaa caaagctttc aagccaacca
     2041 ggacggattt tgactggctg acaagagacc acgaaagagt tgtggattat gtgaaagatg
     2101 atttgtgcgg ttttactttt acatctaaag catattttga gatgttcaag gttttgaaag
     2161 aagtaaatag ctcaaaatgg gcaaaaagcc tagacaagga tttaaaatat ttgtttgtat
     2221 caggggacag ggatccggtt tcaaacaacg gaaaaggtgt tttgaagctt gtcgagagga
     2281 tgaagaaggc aaaagtcaag gatgtaaggg tgaaactgta cgaagactgc aggcacgagc
     2341 ttctgaatga gctgaacagg gaaatggttt ttaaggatat tcttgatttt atcgaggagg
     2401 cagtaagttg aaaaagtatg tcattagtct tgaccagggt accacatcgt caaaggcgct
     2461 tctttttgat agagacttga atctgattca caagaagcag aaagagctga ctcaaatata
     2521 tccgaatgcg ggatgggttg agcatgatcc taaggaagta tatgagtcgt ccttcagtgt
     2581 aattaatgat gttataaaag aggcgggaat ttctgctgcg gaaatttgtg caattggcat
     2641 tacgaaccaa agggagactg caatcgtctg ggacagatac actggagagc ctgtctacaa
     2701 tgccattgta tggcagtgca ggcgaagtgc ctccatgtgt gacgagatta agagacgcaa
     2761 ggagcttttt ggttatataa aagagaatac aggtttgata gttgacgcat atttctccgc
     2821 cacaaaggtt aagtggattc ttgacaatgt ggaaggtgcg tatgacaggg ccaaaaacgg
     2881 agatttgctt tttggtactg tagattcctg gcttttgtac aatttcacaa aaaagcacat
     2941 gacagatagt acaaatgcct cgaggacaat gctttttaat ataagggaca tgaagtggga
     3001 tgaaaggctt ctttcagagt ttgatattcc aaaaagcatg cttccggagg tttgcccttc
     3061 agggcatgta tttggagaaa cgacttgttt tggccatacg atacctattg cagccttaag
     3121 cggagaccag caatcctcct tgtttgggca gaattgtttt gaaaaaggcg atatgaagaa
     3181 cacctatgga accggatgct ttttgctggt aaatacaggc gatgagttta tacaaagcga
     3241 cagcggcatg ataacaactc ttgcatcgga tgtgggcgtc atgaggaagt atgctgttga
     3301 aggcagcgtg tttacaggag gttctgtaat ccagtggctt agggacgaac ttggaatact
     3361 tgagagttca gcccagtcgg aagagtttgc cttaaaggtg aaggacagtt tgggcgttgt
     3421 ttttgttcct gcatttacag gtctgggtgc accttattgg aacatggatg taagaggctc
     3481 catgtttggc ataaccaggg ggacgaacaa gtatcatatt gcaagagccg ctcttgaatc
     3541 catagcattc cagtgtaggg atgttattga agtcattaaa caggatgcca aggtggacat
     3601 atcaagtctc aaggtggacg gaggagctac aagcaacaac ttcctcatgc agttccaggc
     3661 agatataaca aatatggaga ttgtaaggcc gaagatgacg gaatccaccg gttttggtgc
     3721 tgctttgctt gcgggtctta cggttgggtt ctttgaatca aaggatgaga taagacagaa
     3781 atacaaggta gacagaaagt ttgtgccgga aatgacgaag gatgaaagac gtaaaataat
     3841 tgacagatgg aaatgggcag taggccttgt tacacaaaaa tgaagtaata tttgacaaat
     3901 ggaattggac atattagaat aaggttaaac tgaatagata cgtagtaatg aatgctcctt
     3961 atacgaatta acgaataaaa gtctgcgtta atttttgtga ggagcttttt tgtatattac
     4021 cccctcaagg aggttccccc gaatgaaaac catctctatt ctaagtgcaa aagagcgctt
     4081 cctttccaag tttccctgca atatcctgaa ggaatacccg gtagatattg aaattgccag
     4141 ggaggacaag ctgcttgaaa ggatagaagg catatttgaa aggaacggca gggacttgtt
     4201 cacattaatg tccctgaatg aacagggcga actgtcaaat gccaccacac ttatatatca
     4261 ggacaaggca tatgtattga taaaggatgt gccaaaactg acggattttt acgtaaccgg
     4321 cgctgtgtat actgaaatgg cgagaattta tgtaacaaga agctgctgtg taaaaagaag
     4381 cgacatactg caattttcct ttaatgcaaa ctgccttatc gggtacggat tctggattga
     4441 atacgccacc aaatttatag ccaa
//
LOCUS       DAONVY010000148         4457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_13962_length_4457_cov_4.036347, whole genome shotgun sequence.
ACCESSION   DAONVY010000148 DAONVY010000000
VERSION     DAONVY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13962_length_4457_cov_4.036347"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="PLI11_05135"
     CDS             <1..1743
                     /locus_tag="PLI11_05135"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPZ52282.1"
                     /translation="GTGAASGVVVGILLTASTMSTGGQFSATIISLYAFCGFLAGLFN
                     RYKKVGVSLGFFLGNFILSILLTATPEIIINVYEIFFAMIIFIIIPSKFTEFLKLPQL
                     TGVKIPTVRVNYAEKLRQVASERLNGFAGILSKMSEVCREINTHSLENTQKDMVQIID
                     RITDRVCAKCALNSDCWERNFYKSYQAFTAVTEILESKGRILPEEVPKNLSSKCPNLN
                     SILFEAKSSFEIWRIERLWRKRVEECKSVLPAQLAGMSEILTSLADEVDLSVNFVESL
                     EKDIMREFALQDLKIRNVTVSSNKYGRYEVVADVKCCKSKKQCIYSYQKIISKVLGVP
                     MALQTPTCQNTGDNLWCTLVYTEEEPLNISVKVQSVKAHGSEISGDSYLFTELDKGIY
                     LVCLSDGMGSGEIARKQSESVITLIELFMESGLSFPAAVSMIDSLLETGVDKVMSATV
                     DLAVIDLYQGVASFMKLGAVPSVIKKKDKVETVVIASLPMGFVQGAENEYQTVTRKLT
                     AGDYVILLSDGVMEAFREADYTDIEFYAYIESIKAANPQNIADLIIEKAMELSCDAPK
                     DDMTVIVSKIWQKG"
     gene            1789..2250
                     /locus_tag="PLI11_05140"
     CDS             1789..2250
                     /locus_tag="PLI11_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HPZ52283.1"
                     /translation="MAVIDVLVECFNNMMDGLYYYYDRRNGEIVEIYIMHLELAHKYL
                     NGDNLSYIEEWERKVVLETVDFLKNIRFYVKLPARYDVDEYRIMARFCYTRKKKKHVN
                     KLMDALSGKGRMRTFNETVKKLRLVEEWKEFRHKELVNIAIKWCEENGIPY"
     gene            complement(2257..3507)
                     /gene="larA"
                     /locus_tag="PLI11_05145"
     CDS             complement(2257..3507)
                     /gene="larA"
                     /locus_tag="PLI11_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPZ52284.1"
                     /translation="MILEFGYGEGIQKVEVPDENVIDILKPNKVEYDRIDEEEVKRAL
                     LNPIGSKRLKDIVKPGEKIVVITSDITRPMPTYKVMPLLLDELYEAKVNPKDITLVFA
                     LGSHRKHTREEMIKLAGERAANEINMVDSDMRDCVRMGVTEKGTPVDIFRLVAEADRR
                     ICLGNIEYHYFAGYSGGSKAIMPGVSTREAIRKNHSLMVEEGSHAGNLDTNPVRQDIE
                     EAASLCPIDFILNVILDEKKTIIKAVCGHPVLAHREGAKFLDKLYLKEIKERADIVLV
                     SQGGKPKDINLYQTQKALDNAKHAVKKGGTVILIGECTEGLGEKVFEEWMTKSKSPDE
                     MIERIRKDFQLGGHKAAAISLVLKDADVYLVSAMDEELVRSMFLKPFKSAQDAFDTAF
                     KIHGENATVIAMPYGGSTLPKEIK"
     gene            3976..4137
                     /locus_tag="PLI11_05150"
     CDS             3976..4137
                     /locus_tag="PLI11_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52285.1"
                     /translation="MEKIKSLTIRINAEKWQKAKVKMLIEGKGRTFQDICEEAIDRYI
                     EESDDKEKK"
BASE COUNT         1326 a          834 c         1017 g         1280 t
ORIGIN      
        1 ggtacaggag ctgccagtgg tgtggttgta ggaatacttc ttaccgcttc caccatgtcg
       61 acaggtggac agttttctgc gaccatcatc tctctgtatg ctttctgtgg ttttcttgca
      121 gggcttttca accgctacaa aaaggttgga gtaagcctgg gtttcttcct tggaaacttt
      181 attctttcca tacttctgac agccaccccg gaaatcataa tcaatgttta cgaaatattc
      241 tttgcaatga taattttcat tatcattcct tcgaaattta cagaattttt gaaactgcct
      301 cagctgaccg gtgtaaaaat accgacagtg agggtaaact atgctgaaaa gctcaggcag
      361 gtggccagtg aaagattgaa tggctttgcc ggcattttga gtaaaatgtc agaagtctgc
      421 agggagataa acacccattc gctggaaaac acgcagaagg acatggttca gataatagac
      481 aggattactg acagggtttg tgcaaaatgt gcattgaact cggattgctg ggaaagaaac
      541 ttctataaat cctatcaggc ttttaccgca gttaccgaga ttctggagag caagggaagg
      601 attttgcctg aggaagttcc aaagaacttg tcatcgaaat gtcccaattt aaacagcata
      661 ctttttgagg caaaaagttc gtttgaaatc tggaggattg aaagactctg gagaaaaaga
      721 gtggaggagt gcaaaagcgt actgccagca cagcttgcgg gcatgtctga gatattgaca
      781 agccttgccg atgaagtgga tttgagcgtg aactttgttg aaagtcttga aaaggatatc
      841 atgagggaat tcgcattgca ggacttgaag ataaggaatg tcactgtctc cagtaacaag
      901 tatgggcggt atgaagtggt ggcagacgtg aaatgctgca agtcaaaaaa acagtgcata
      961 tattcctacc agaagataat ttccaaagtg ttgggagtac caatggccct tcaaacgccc
     1021 acctgtcaga atacagggga caacctctgg tgcacccttg tatataccga agaagaacct
     1081 ttaaacatct ctgtaaaggt gcagagtgtg aaggcgcatg gaagcgaaat tagtggggac
     1141 tcatatcttt ttacagagct tgacaaggga atttatcttg tatgtctgag cgatggcatg
     1201 ggcagcggag aaattgcacg caagcaaagc gaatcagtta taactttaat agagctgttt
     1261 atggagagtg gtcttagttt ccctgcagcg gtgtccatga ttgattctct tcttgaaacc
     1321 ggtgtggaca aggttatgtc cgccacggtg gatctggcag ttattgattt gtaccaggga
     1381 gttgcatcct tcatgaagct gggcgctgta cccagtgtta tcaaaaagaa ggacaaggtg
     1441 gaaacagtgg ttattgcatc gctgccaatg ggatttgtgc agggggctga gaacgaatac
     1501 cagacggtta caagaaaact gacagcagga gattatgtaa tacttttaag tgatggagtg
     1561 atggaagcat tcagggaggc tgactatacg gatattgagt tttatgcata tatagaaagc
     1621 attaaagctg caaatccaca gaatattgcg gacctgataa ttgaaaaggc catggagttg
     1681 tcttgtgatg ctccaaagga tgacatgact gtcatagtgt cgaaaatatg gcaaaaaggc
     1741 tgagttgttg atataatcta aatgaaagaa acttacgggg tagttgttat ggcggtaatt
     1801 gatgtattgg tggaatgctt caacaatatg atggatggac tatactatta ttacgataga
     1861 agaaatggtg aaattgtcga aatatacatc atgcacttgg agttggcgca caagtatttg
     1921 aatggtgaca atctttcata tatagaagaa tgggaaagaa aagtggtact ggaaacagtg
     1981 gattttttaa agaacattcg attttatgta aaattgcctg caagatacga tgttgatgaa
     2041 tacagaatca tggcaaggtt ttgttatacg agaaagaaga aaaagcatgt aaacaagctt
     2101 atggatgcct tgtccggcaa gggtcgaatg aggactttta acgagactgt aaaaaaactc
     2161 aggcttgttg aggaatggaa ggagttcagg cataaagagc ttgtaaatat tgcaattaaa
     2221 tggtgtgaag aaaacggcat cccgtattga tgccgtttat ttgatttctt ttggtagtgt
     2281 tgaaccgcca tagggcatgg ctataactgt ggcgttttcg ccgtgtattt taaaagctgt
     2341 gtcaaaagca tcctgggcag atttaaatgg ttttaaaaac atgcttctta ccagttcttc
     2401 atccatggcc gaaaccaggt aaacatccgc atctttcagt acaagcgata tggctgcggc
     2461 tttgtgacct cccaattgaa aatcttttct gattctctca atcatttcat cagggctttt
     2521 ggactttgtc atccactctt caaagacttt ttcaccaagc ccttcagtac actccccgat
     2581 tagtattaca gtaccgccct tctttactgc atgcttggca ttgtccaatg ccttctgtgt
     2641 ctgatacaag tttatatcct tgggctttcc tccttgagat acaagaacaa tgtcagctct
     2701 ctcttttatt tcttttagat acaacttgtc caggaatttt gcaccttccc tgtgggcaag
     2761 cactggatga ccgcataccg cttttattat cgtcttcttc tcatccaata tcacattcag
     2821 gataaagtcg atagggcaca ggctggctgc ttcttcaata tcctgtctga ccgggtttgt
     2881 atccaggttt cctgcatggg aaccttcctc caccataagg gaatgattct tccttatggc
     2941 ttctcttgtg gaaactcccg gcatgattgc cttggaaccg ccgctgtatc cggcaaaata
     3001 gtggtattca atatttccaa gacatattct tctgtcagcc tctgccacaa gtctgaatat
     3061 gtcaacgggg gtaccttttt cagttactcc cattctgaca cagtcacgca tgtcgctgtc
     3121 taccatgttt atttcgttgg cggctctttc gcctgccagt tttatcatct cttcccttgt
     3181 atgttttctg tgactgccca aagcaaatac aagagtgata tcttttggat tgacctttgc
     3241 ctcatacagt tcatccagca atagaggcat gaccttgtag gtgggcattg gtcttgtaat
     3301 atcgctggtg ataacaacta tcttttcgcc aggcttgact atatccttaa gtcttttgga
     3361 gccgatggga ttcaaaaggg ctcttttcac ttcctcttca tcaatcctgt catactccac
     3421 cttgttcggc tttaatatat ctattacatt ttcatctggt acttcaactt tttgtattcc
     3481 ttctccataa ccaaattcca gtatcattgc atcaactcct gatatcaaat acttctaaag
     3541 tatacttgat ttttgcaccc tttacaagac gatttgaaag gtagccaatc taaatgatat
     3601 actatacctg gaaaaggcat ggaggtgaaa aaatgtcaat ggatacgctg gttaacatat
     3661 tcccatacct aacacatgtc gagtgcgtgg tagagataca aagaagcgaa tcgtcgaaat
     3721 agcacatttt acaaaggtta cagactatta tcagtttact agaaccggcc atgattttcg
     3781 aaacaaggtg ccagtttcgg agctagaaat taggatagcc ttgttaatat cgtgtgagta
     3841 aacacaaaaa atacatacat atttacccgc tcaaaaagag cgggatttta acttttttgt
     3901 aagaaaattg taatatttgg atgcttgact tcatgagctc tagattgtat aatgaaattg
     3961 gcaggaggga ggaaaatgga aaaaataaaa agcttgacta tcaggattaa tgcagaaaaa
     4021 tggcaaaaag caaaagtcaa gatgcttata gaaggaaaag gaagaacttt tcaagacatt
     4081 tgtgaagaag cgatagatcg ttacatcgaa gaaagtgatg acaaagaaaa aaaataaata
     4141 gaactcttga cttctagagc tcaatatgtt aaaatataag tgaaagagat aagtaaataa
     4201 atttttcggc acggtggagc tagccgctaa agcaacgaaa ccgggccgaa cccagggcgg
     4261 acgctacttc gaggaccacg ggggtccaat gagaagaatg cgctgggggc tgaatacccg
     4321 gaatttatgc gagtacgcaa agataaaaga tattttgcgt ttttgcataa gttccgggtt
     4381 tttgttttgt acatatgcaa aaaaaacaaa ataagaggcc ctgaagaggg cggacgtggc
     4441 accggagccg tgaggcc
//
LOCUS       DAONVY010000149         4443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14013_length_4443_cov_3.540109, whole genome shotgun sequence.
ACCESSION   DAONVY010000149 DAONVY010000000
VERSION     DAONVY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14013_length_4443_cov_3.540109"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PLI11_05155"
     CDS             <1..1061
                     /locus_tag="PLI11_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52286.1"
                     /translation="NLRYFYSDLLFEACLHTVCSRTEENANKAKEQYGFKYEVTDYDK
                     VVLNPEIDVVSICTPNSMHAEQIIKAVRNGKHVYCEKPAVTTRDEAIMVQNVLKDTQN
                     VCGIVFNNRYFPATMRAKQLVEEGRIGNVVGFSGRYLHSSGLNRSSYGWRGDRKMSGG
                     GALYDLGSHVVDLITHITGNRIKSVIGKGHILNKAITDCDEHFSMIARLENGGLGTIT
                     VSRITIGTNDDLEIEIHGDKGAIKFNLMEPNKLYFFDGSKKTSPYGGESGYTVIDCIG
                     KYEYPGGVFPSSKAPVSWLRGHVHSMYIFLQSVYEGDTELEKGAKLLDSLNINCILEA
                     ALVSNETGKEEEVRYINN"
     gene            1076..1789
                     /locus_tag="PLI11_05160"
     CDS             1076..1789
                     /locus_tag="PLI11_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52287.1"
                     /translation="MKDKFYYNFVNSLIAIGILSIPISILMYILFETGIIVTNINNFV
                     VLVIGGLVCILSFVLHFVLSKTYGYKKVMWKYKEENEIELPFDNLSEGFGKSLKDLGY
                     HVVSLPDLFDVFVLEEPLKDGLYIKAAFCVKEEEFNLKKVRSLENTLTRYYLDNDMAI
                     KAITGICCVVLDGVNKRKVDKYKQVRDYFNYNESKDIVAQIAVCAADLHNRKVYFNSE
                     NITGKRNIPILLINDIFKR"
     gene            1813..2247
                     /locus_tag="PLI11_05165"
     CDS             1813..2247
                     /locus_tag="PLI11_05165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPZ52288.1"
                     /translation="MEAYVHKVQYYETDQMGVVHHSNYIRWFEEARIDYLDKMGMSYD
                     AVEKKGIISPVVSLNCEYKSPARFGDTVFIVTCLKMVSGVKFCFGYEVYDLKSREIRA
                     TGESRHCFLKDNKIISIKKENEEVYELFKSYEGVSTTIEGIY"
     gene            complement(2228..4054)
                     /locus_tag="PLI11_05170"
     CDS             complement(2228..4054)
                     /locus_tag="PLI11_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPZ52289.1"
                     /translation="MKKVLKDSNQYIKDSIVDYGDFKLFLDLDNGFSKNNLIPPKQGS
                     EHIIKKAEEYLNHPYPQQTASMYMEFKKNGNRTNYEKPYFKRRDMLFYFLLAECIERK
                     GRFTDNLIDLLWMILEESTWVLPAHNGHMPHSSYICPLPYCFKDEVDYIDLFAAETGA
                     LLAWVYYLAEEILNSITPVIASRLLYELDRRIINPFLINDDMWWTSLKGNAVNNWNPW
                     IVSNALVVCALCVKDTNKRGVFVERACRMLDGFTKHYPDDGGCDEGPSYWNVAGASYF
                     DCLELIYDMTNGRIDVFSNPFVGRIMEYIMHMHVVDDFYLNFADSPAKITVDFSAVAR
                     MGRRTKSNDLEAFGLAQYNLTKPCIISQRTYRSIKSLYDVPQKQGAEYTAKDAALPQL
                     GVMVLRSDKSLLGIKGGHNAEGHNHNDVGNFIVFANSNPLIIDVGVGTYTKETFSSQR
                     YSIWTMRSEYHNLPNIAGHEQIAGFAKRATNCVFDLHNRTFRAELKETYGFEKLVSYT
                     REARMYEDGCIEIIEDIKMEEKSDVNFHLMTIDRPKIGENCLIFENGKVEFDKSLEVF
                     VEQIEMNDEKLSGVWKTNSVFRIHLKAKDIKEGIYHFNIFPQ"
     gene            4163..>4443
                     /locus_tag="PLI11_05175"
     CDS             4163..>4443
                     /locus_tag="PLI11_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPZ52290.1"
                     /translation="MRKYRIELHSHTSEVSPCGKITAQEMIELYVQKGYNGIVITDHF
                     SGPLFSDLEDIGDILARFCKGYKNALLAAQDRIKVYLGAEVRLDESYND"
BASE COUNT         1286 a          751 c         1012 g         1394 t
ORIGIN      
        1 ctaatctcag gtatttctat agtgacttgc tgtttgaggc atgtctgcat accgtgtgct
       61 cgagaactga agagaatgca aacaaggcta aagagcagta cgggttcaaa tatgaagtta
      121 cggattatga taaggttgta ttaaaccctg aaatagatgt ggtatccata tgcactccca
      181 acagcatgca tgcagagcag attattaaag ctgtaaggaa tgggaaacat gtttattgtg
      241 aaaaacctgc tgtcaccaca agggatgagg cgattatggt gcaaaacgta cttaaagata
      301 cacaaaatgt ctgtggtatt gttttcaaca acagatattt ccctgccacc atgagggcga
      361 aacaattggt ggaagagggt agaataggta atgtggtggg ttttagtgga aggtatcttc
      421 attcctccgg attgaacagg tccagctatg gatggagggg agacaggaag atgagtggtg
      481 gcggcgcttt gtatgatttg ggttcccatg tggtggatct gatcactcac attacaggaa
      541 acaggataaa gtcggttatt ggcaaggggc atatactgaa caaggcgata acggattgcg
      601 atgagcattt ttccatgata gcaaggcttg aaaatggagg ccttggaact attacagtaa
      661 gcagaattac cattggcacc aatgatgatt tggagataga gatacatgga gacaagggag
      721 caatcaagtt taatttgatg gaaccgaata aactgtattt ctttgatggc tcaaagaaga
      781 caagtcccta tggaggagaa tctgggtata cagtaattga ctgcataggc aagtacgagt
      841 atcctggagg ggtgtttcca agttccaagg ctcccgtcag ctggcttaga ggacatgtgc
      901 attcaatgta catctttttg cagagtgtgt atgaaggcga cacagagctt gaaaaggggg
      961 caaagctttt ggattctctg aatattaact gcattcttga agcggcgctg gtttccaatg
     1021 agacgggcaa ggaagaagaa gtaaggtata ttaataattg aaggaaggtt tatttgtgaa
     1081 ggacaagttt tattataatt ttgtcaactc tttgattgcg attggaattt tatcaattcc
     1141 cataagtatt ttaatgtata ttctttttga aacaggtatt attgtcacca atatcaacaa
     1201 ctttgttgtg ttggtcatag ggggtttggt ttgcattctt tcttttgttt tgcactttgt
     1261 gctttccaaa acgtacgggt acaaaaaagt gatgtggaag tacaaggagg aaaatgaaat
     1321 agaactgcct tttgacaatt tgtctgaagg ctttgggaag tcgttgaagg atcttggata
     1381 ccatgtggta agtcttcctg atttgtttga tgtttttgtt cttgaagagc ctttgaagga
     1441 tggtttatac ataaaggctg ctttttgtgt taaagaggaa gaatttaatt taaaaaaagt
     1501 cagaagtttg gagaatacat tgacaaggta ttatcttgat aatgacatgg ctatcaaggc
     1561 cataaccggc atatgctgtg ttgtactgga tggggtaaac aaacgcaagg ttgacaaata
     1621 caagcaggtt agggattatt tcaattacaa tgaatcaaag gacattgttg cgcagattgc
     1681 ggtttgtgca gcagacttgc ataataggaa ggtttacttt aattccgaga acataacagg
     1741 caagagaaat atcccaattc tactgattaa tgatattttc aagagataaa ttggaggatt
     1801 gtagtaggag caatggaagc atatgttcat aaagtgcagt attatgagac ggaccaaatg
     1861 ggagtggttc atcactcgaa ttatataagg tggtttgaag aggcaagaat tgactatttg
     1921 gataagatgg gaatgagtta tgatgccgtt gagaaaaaag gaatcatatc acctgttgtg
     1981 tctttaaact gtgaatataa atcacctgca agatttggtg atacggtttt catagtgaca
     2041 tgcctgaaaa tggtcagtgg tgtaaagttt tgctttggtt acgaggtata tgacttgaaa
     2101 agcagagaaa tacgggcaac aggcgaaagc cgccattgtt ttctgaagga taataaaatt
     2161 atctccataa agaaggagaa cgaagaagtt tatgagcttt tcaagtcata cgaaggagtc
     2221 tctactacta ttgagggaat atattgaagt gatatattcc ttctttgata tcctttgctt
     2281 tcaaatgtat tctgaatacg ctgtttgttt tccacacgcc tgaaagtttt tcgtcattca
     2341 tctctatttg ctctacaaaa acttcaagag atttgtcaaa ctctaccttt ccgttctcaa
     2401 agattagaca gttttcacct atctttggtc tatcaattgt catcaggtgg aaattcacat
     2461 ccgatttttc ctccattttt atgtcctcaa taatttcaat acagccatcc tcatacattc
     2521 tggcttccct tgtgtaggaa acaagtttct caaaaccgta ggtttcttta agctctgctc
     2581 tgaaggttct gttatgcaaa tcaaatacgc aattggtggc tctttttgca aaacctgcaa
     2641 tttgctcgtg tcctgcaatg ttgggaaggt tgtggtattc cgaacgcatt gtccagatac
     2701 tgtatctttg ggagctgaag gtttcttttg tgtaggtgcc tacacctaca tctataatga
     2761 ggggatttga atttgcaaag acaatgaagt tgccaacatc attatggttg tggccttctg
     2821 cattgtgtcc tccctttatt cccaaaaggg atttgtcact tctcagaacc ataactccaa
     2881 gctgggggag tgcggcatct tttgccgtgt attctgcgcc ttgtttttgc ggcacatcat
     2941 aaagggattt tattgacctg tatgttcttt gcgatattat gcagggtttt gtgagattat
     3001 attgtgcaag tccaaaagct tcaaggtcat ttgattttgt tcttcttccc attcgggcaa
     3061 ctgcagaaaa gtccactgta atttttgcgg gactgtctgc aaagttgaga taaaaatcat
     3121 caacgacatg catgtgcatt atgtattcca ttattcttcc tacaaatgga tttgaaaaca
     3181 cgtctattct gccgtttgtc atatcataaa tcagttcaag gcagtcaaaa tatgaagcgc
     3241 cggcaacgtt ccagtaggag gggccttcgt cgcatccgcc atcgtccgga tagtgtttag
     3301 taaagccgtc cagcattctg caggctctct ctacaaaaac tcccctttta tttgtatctt
     3361 ttacacaaag tgcacagaca acaagagcat tggatactat ccaggggttc cagttgttca
     3421 cagcatttcc ttttagtgaa gtccaccaca tatcgtcgtt gataaggaac ggatttatta
     3481 tccttctgtc cagctcatat agcagcctgc ttgctataac gggggttatt gaatttagga
     3541 tttcttctgc caggtagtat acccatgcaa gaagagcacc ggtttccgcc gcaaaaaggt
     3601 cgatatagtc gacttcatct ttgaaacagt agggaagagg gcatatataa cttgagtggg
     3661 gcatgtgacc gttgtgggca ggcaacaccc aggtgctttc ctcaagtatc atccataata
     3721 aatcaatcag attgtctgtg aaacgtccct ttctttcaat gcattcggca aggagaaagt
     3781 aaaagagcat gtcgcgtctt ttgaaatacg gtttttcata gtttgttctg tttccgtttt
     3841 ttttgaattc catgtacatg gaggctgtct gttgtggata agggtgattc aaatactctt
     3901 ccgctttttt tataatatgc tcactgcctt gttttggcgg aataagattg ttttttgaaa
     3961 aaccgttatc caggtcgaga aacagtttga aatcaccata atcaacaata ctgtctttta
     4021 tgtactgatt gctgtctttt aaaactttct tcatgttcct cagatccttt tcattttttt
     4081 caatattaac actttatata gcaattatca acatgtattt gctacaatta aaaagcaata
     4141 tttaactggg agatgaaggt gtttgagaaa gtacaggata gagttacata gccataccag
     4201 tgaagtgagc ccctgtggga aaattacggc tcaagaaatg atagagctgt atgtccagaa
     4261 gggttataac gggatagtta taactgacca tttttccggt cccttgttta gcgatttgga
     4321 ggatatcgga gatattctgg caaggttttg caaagggtac aaaaacgcgc ttttggctgc
     4381 ccaggataga ataaaagttt acttgggtgc ggaagtaagg cttgatgaat catacaacga
     4441 tta
//
LOCUS       DAONVY010000150         4368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14286_length_4368_cov_3.210526, whole genome shotgun sequence.
ACCESSION   DAONVY010000150 DAONVY010000000
VERSION     DAONVY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14286_length_4368_cov_3.210526"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..2370)
                     /locus_tag="PLI11_05180"
     CDS             complement(214..2370)
                     /locus_tag="PLI11_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52291.1"
                     /translation="MFTDYTKGMITEYFVRGAVSHIYRGDRGYPLTIHYRNGRKIVHR
                     SIPCFVVYCRMSFDGKLLYVGNENCVLHCFDSETLDPLDPRLLGMFDNEGAVVNKENF
                     NIFKGLMLTEYEFKAGDYCTQCNSANDVRYSNFDGSILICQKCFNDFYRMMYFRIIDM
                     LADESLAEKRRVKMTSEYTRTECKKEGIHHISAYIKVETSDDVKYYLTHNHPDNYLKS
                     LCYQDKYRDTKITICGLHDDICYEIFIDPENKVKTYIYQPEAISREDREGYKELIKDL
                     SDELLENPFTERLTEYELIQTFSYSTQTPFSNMNVLFKILKDTMDHFGERELLLYTFS
                     DGKIKDCYKYDITKDFKISGYLNDVGGSFLLFSEEKRFAIIHSGYGFAAVGGTYEFMK
                     KFRQFMIKNGIYGTQDFVRYYRLYKKDMNSFYARKRKIVTWATSQKSQAGLYDILHTH
                     IFSSNNRRLIRNDTICHCFYCLKQFEGSKINEWINDRNGKTAVCPFCGIDAILPETCG
                     QKLDEDFLIKMKEYWFESDKLIQINSKLQTYETYYSFILLDTDSLNIILNGIQCYLDG
                     KYILCMNKSDVIQEISGRYTVMNLSFSPFVLNLNINKENNINACDLYLFSQRNEKYKG
                     IISLNNDEYDTIILYFKRIERFMDHKEYLWVCKVSKDMSSILNVAECAFSNRRAPEGN
                     EIIRYIKDHIRDNLTLTKLCEQFHMSRTTLAKIYMI"
     gene            complement(3067..3525)
                     /locus_tag="PLI11_05185"
     CDS             complement(3067..3525)
                     /locus_tag="PLI11_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HPZ52292.1"
                     /translation="MPKVILTNMVMIEDRKNNKVLVQDRIKSYKGYSFPGGHVKGDES
                     FYDSAVREVKEETGLDVSNLSFCGIIHWLNKTNEDRYIVFLYKTHEFKGTLLEKSEEG
                     RNIWISLDELVNSPFTNNLVDYLPMFLDKGYGEAFAPYTIEDEIENVFFK"
     gene            complement(3620..4165)
                     /locus_tag="PLI11_05190"
     CDS             complement(3620..4165)
                     /locus_tag="PLI11_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPZ52293.1"
                     /translation="MECISKDLRKEHDAILFGLTILEHLAELVRAESGYDVDDLKGMV
                     DFFRYFADKCHHGKEEDILFPAMEIAGIPKVNGPIGVMLNEHEVGRKLIKGMDSAITG
                     NEVDKEEFANAADLYVELLRSHISKENNVLFPMGDRRIPVSRQKELQEEFDKFEKEIM
                     GEGKHEEVHSFIEDMEVKYLH"
BASE COUNT         1321 a          883 c          676 g         1488 t
ORIGIN      
        1 tatttactta aatttaaatt taatttcttc tttctaggcg atattgtagc ggtgattttc
       61 catacctttt tctagagcat cgaataaaat aattatcacc tgaaaaacca tatttttctg
      121 ccagtacatt aacaggtata tctgtgaaaa caagctcagg caacacctgt gtaagcctct
      181 cttcaagtac ataggacatt ggtgatgttc ctgtcagatc atatatattt ttgcaagcgt
      241 tgtcctgctc atatgaaact gctcacataa tttcgttaaa gtaagattgt cccttatatg
      301 atccttaata tacctgatta tttcattacc ttccggtgcc cttctgttac tgaatgcaca
      361 ttctgccaca ttgaggatcg aactcatatc cttgcttact ttgcagaccc ataaatattc
      421 tttatgatcc ataaaccttt caatacgctt gaaatacagg ataattgtat cgtattcatc
      481 attattcagg gatattatcc ccttatactt ttcattcctc tgactaaaaa gatataaatc
      541 gcaagcatta atattgtttt ccttgtttat attaaggttc agtacaaagg gtgaaaagga
      601 aagattcata acagtatacc tgcctgatat ttcctgaatt acatcacttt tattcatgca
      661 taaaatatat ttgccatcca gataacactg tataccgttt aaaattatat ttagagagtc
      721 ggtatccagt aatatgaagg agtagtatgt ttcatatgtc tgaagtttag aattgatctg
      781 aatgagttta tcactttcaa accagtattc cttcattttt atcaaaaagt cttcatctaa
      841 cttttgacca caggtttcag gtaatatggc atcaatcccg cagaaaggac ataccgcagt
      901 cttcccgttt ctgtcattga tccattcatt tatcttcgat ccttcaaatt gctttaagca
      961 atagaagcaa tggcatatag tatcatttct tattaaccgc ctgttgttag aagaaaaaat
     1021 atgggtatgc agtatatcat agagccctgc ctgagacttt tggcttgttg cccaggttac
     1081 tatttttctc ttccttgcat aaaaactatt catgtccttc ttatataacc ggtagtatct
     1141 tacaaaatcc tgagtgccat atatcccgtt ctttatcata aattgtctga actttttcat
     1201 aaactcatag gtaccgccta ctgcagcaaa tccatagcct gaatgtatta tcgcaaacct
     1261 cttttcttct gaaaataaca gaaaggatcc tccaacatca ttaagatatc cagatatctt
     1321 aaaatccttt gtaatatcat acttatagca gtcctttatc tttccatcag aaaaggtata
     1381 caaaagtaat tctctttcac caaagtgatc cattgtgtcc tttaatatct taaaaagaac
     1441 attcatattt gaaaatggtg tctgtgttga atagctaaaa gtctgaatca actcatattc
     1501 agtaagcctt tctgtaaatg ggttttccaa tagctcgtct gataaatcct ttattagttc
     1561 cttatatcct tcacgatcct cacggctgat tgcttctggc tgataaatat atgtttttac
     1621 cttattttca ggatcgataa atatctcata acatatatca tcatgtagtc cgcatatggt
     1681 tattttagta tccctatatt tatcctgata gcaaaggctt tttaagtaat tatcaggatg
     1741 attatgagta agatagtatt ttacgtcatc tgaagtttct actttaatat atgcagaaat
     1801 atgatgtatt ccctcctttt tgcattctgt tcttgtatat tcagatgtca ttttcactct
     1861 ccgtttttca gctaaacttt catctgccag catatcaatg atacggaaat acatcatccg
     1921 gtaaaagtca ttaaaacatt tttggcaaat caatatagat ccatcaaaat ttgagtatct
     1981 gacatcattt gcactattac actgggtaca gtaatcacct gccttaaatt catattcagt
     2041 cagcattaaa cctttgaaaa tgttaaaatt ctctttatta acaactgcac cttcgttgtc
     2101 aaacatccca agaagcctgg gatcaagggg atctagggtc tctgaatcaa agcaatggag
     2161 tacacaattc tcatttccta catatagcaa tttgccatcg aaggacatcc tgcaatatac
     2221 gacaaaacac ggtatgcttc tatgtactat tttacgtccg ttacgatagt ggatagtaag
     2281 aggataccct ctgtcgcctc tgtatatgtg gcttacagct cctctgacga agtattctgt
     2341 tatcatacct tttgtataat ctgtaaacat gtaaatcccc ctttattgat ctaatatata
     2401 tataatatca gcctatttga gaactttgtg tacttaaaat agcacgattt acaactttac
     2461 atttacagat tactatttat tataaggata gacaatattt agtctattaa atgaaaaaaa
     2521 tatggtttta tgccacaaat aatataaagg aatccgtatt aaggatgcat atgcaaagcg
     2581 tttggaccgt ttatggcata gcggttgatg aggatgggga gtgtcgaaag actcggcggt
     2641 tgctccacgg cagacctgtc gttaaacgtc actaaaaaat ccgggtaacc ggaaaacaaa
     2701 ggcaccgtgc gagataaatg gaatagaccg cagtccactt ttaagggaga caaacaaggc
     2761 aataggtctc aactagctgc cttggataac ggatgtaaaa atattgagca aagaagaaat
     2821 cattaaaacc acaagcatgg ctttcttctt cattgtgtgg ccaaaaaagg tttcattgcc
     2881 atatacaagt atggctgaaa tacaaagagt ggtgtatgta agtattttcg ttagaaaaac
     2941 agtgtgctta tgtaagatca tttacagctg tcttggcaac atccttaggc agaaggaaaa
     3001 ttgagtagca aatgaaatac aaaaatatat gaagtcttct tgattctctc tttcatgtaa
     3061 cctcccctat ttgaaaaaaa cattttcaat ctcgtcttct attgtatatg gagcaaatgc
     3121 ttcgccatac cccttgtcca aaaacatggg caaataatca acaagattgt ttgtgaaagg
     3181 actatttaca agttcatcaa ggctaatcca tatgtttcta ccttcttcgc ttttttcaag
     3241 aagggttcct ttaaactcat gagttttgta aagaaataca atatatctgt cctcattggt
     3301 tttgttcaac cagtgaatta tcccgcaaaa actcaagtta cttacatcaa gtccggtttc
     3361 ctcttttact tcccgtacgg cactgtcgta aaagctttcg tctcctttta catgacctcc
     3421 aggaaaggaa taccctttgt agcttttaat cctgtcttgc accagtacct tgttgttttt
     3481 cctgtcttca atcataacca tgttggttag tatgactttt ggcaaataac gttcacatcc
     3541 tttacacaaa accaattata ctacaatatt tttatatttc gttgcatgat ataaaatttg
     3601 ttttgtctgt tattaaatat taatgcagat acttgacctc catgtcttct atgaagctgt
     3661 gaacctcttc gtgcttgccc tctcccatga tctctttctc aaacttgtca aactcctctt
     3721 gaagttcctt ctgtctgctt actgggattc ttctgtctcc catggggaag agaacgttgt
     3781 tttccttgct tatgtgagac cttaaaagct ccacatacaa atcagcggca tttgcgaatt
     3841 cttccttgtc cacttcattg ccagttattg ccgaatccat gcctttaata agctttctgc
     3901 caacttcatg ctcattcagc ataacaccta tagggccgtt cacctttgga attcccgcaa
     3961 tctccatggc tgggaacagg atgtcctctt ccttgccgtg gtgacacttg tcagcaaaat
     4021 acctgaagaa atccaccatg cccttcaagt catcaacatc ataaccactt tcagcacgaa
     4081 ccagctcagc caggtgctcc aaaatggtca atccaaaaag aattgcatca tgttcctttc
     4141 ttaaatcctt gctaatgcat tccatacgat tccctcccca tattttttac tctcatgcgg
     4201 atgtgtatat tataccattt atcccattac ctgcaaccaa tattgttgcc cggtacatat
     4261 attttaccta ttattcttaa aaagaatgtg attttaatca aaatttgaag aaaaagatta
     4321 tgggatatat aattgaaggt aagaagagat ataaggatgt gttgtcat
//
LOCUS       DAONVY010000151         4364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14299_length_4364_cov_3.283128, whole genome shotgun sequence.
ACCESSION   DAONVY010000151 DAONVY010000000
VERSION     DAONVY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14299_length_4364_cov_3.283128"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLI11_05195"
     CDS             complement(<1..335)
                     /locus_tag="PLI11_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52294.1"
                     /translation="MDFNDEEVDYDARNIIDLTDFWQVTLAGSGPVAVVVEDGDNKYL
                     SLQGYMEFFTYDLIKPPYTFSVDVQLVDTENIAFFVRTYEPITKHNPAHGEKDDRIGY
                     FEADWYKENG"
     gene            519..3191
                     /locus_tag="PLI11_05200"
     CDS             519..3191
                     /locus_tag="PLI11_05200"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPZ52295.1"
                     /translation="MKGNQKYVTTIILLLCFLTPALVLLFAFSAYGMAPFGEKSIMIM
                     DMSGQYSEFYIGLRHFGKMGNMFFSWSKTLGTSYIGVFAYYLSSPLSFLTLLSSEANM
                     PIFLLFLTVLKIGLCGMTFGIYLMYMFKRRDFSVVLFSLFYGLMSYNIVYSMCLMWID
                     GVIWLPIILIGIEELLKKGKRKLLVTSLFCVFISTYYISYMIGAFSCIYLVYRYLSHY
                     SDEMPVKRLFIYFTKLISSALIAALLGAWFLIPTLVSLFDGKIGGANYTPQGNINFEL
                     KNLVSKLFLGSYDSITNSGMPFIYCGIVVTILFIGYFFIRSVSVKEKASTFLVASYLL
                     ASMYFINLDVAWHIFQFPNWFPYRYSFVFLFFMIFTAYKAFDSIKDLSKAFFVTFPLV
                     SIMVAQLVLKIRYRYLDEERITLTVKLLVFYIFCLLAINLIKHVKEKEGFDDSVKRYA
                     IAFLTLTMLVISSYELYKHSIYMLKGLDKAHHYEDIKPYQEYRNAVNKLLEEAKSQKG
                     KHDFYRVTKNFSRSINEPISFGYSGIDHYSSAYNRVVNDTLRTLGFSSAYLWITGYGS
                     TIVTDTILNIEYLMSISPVTKRYEKLKTIENISLYRKPEDRNIYGEPKDLSIYRNPIA
                     LPVAFMADSELLRKKLKAPNMFAAQNLFLSHLVGENINCFERITPERYGKYSYPKEDD
                     DYVYMKSGSEGIVSYYFIAEKDMELYAYFPFNNNHTNCSVYVNDVYVANLYYGQYDCA
                     HFLGSFKKGDEARIDLIFKSTYLNTRGNMFYALDYDKFASICDEFREESLKVDYFDGN
                     EIKGTVSAKDNGTLFTSIPYDKGWSVYVDGKKVKTHKYSDTFLCIEVPKGEHEIIFKF
                     THVGFVPGLILSITTLLGIITFEILQRRKLISKS"
     gene            complement(3175..4233)
                     /locus_tag="PLI11_05205"
     CDS             complement(3175..4233)
                     /locus_tag="PLI11_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52296.1"
                     /translation="MKKINVGIIGTGGISHIHMNGYKAIEGVDVVAVCDIDEEKVKAY
                     AARYNVPRYYTDYNEMLEKEELDCVSVCTWNSEHKNATIAALNAGANVLCEKPMAMNQ
                     EEALEMEKAAKDNNKLLMIGFVRRFGNDTDIIKRFIEAGDMGDIYYAKASYLRRNGTP
                     GGWFADKRYSGGGPLIDLGVHVIDLVRYLAGNPLPVSAYGVSYGKLRDKEVAGDKAWK
                     IDSEGAFEANTEDFAAGFVKFENGLTLYIEASFNLHIKHDVGSVELFGTKSGAKIDPY
                     VHIFTDLNDTMVDIEPSGSSAFDFGTIFNKEIYHFVDCVRTGKTCISPAEDGVVLMKI
                     IDALYESARTNKEVKISI"
     gene            complement(4220..>4364)
                     /locus_tag="PLI11_05210"
     CDS             complement(4220..>4364)
                     /locus_tag="PLI11_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPZ52297.1"
                     /translation="LMLSGIQLFSIGILGQYLAKTYLEVKRRPLYIIKDSNIIRREHN
                     EEN"
BASE COUNT         1308 a          945 c          765 g         1346 t
ORIGIN      
        1 ccattctctt tgtaccagtc tgcctcaaaa tatccgattc tgtcgtcctt ttctccatgt
       61 gcaggattgt gctttgtaat aggttcatag gttcttacaa aaaacgcaat gttttcagta
      121 tcaacaagct gtacatcgac gctgaaggta tatggcggct taatcaggtc gtaagtaaag
      181 aattccatat acccctgcag agaaagatat ttgttgtctc catcttccac tactactgcc
      241 acaggaccgg aacctgccaa tgttacttgc caaaaatcag taagatcaat aatgtttctg
      301 gcatcatagt ccacttcctc atcattaaaa tccattttga actcaaaggt tgcagcggac
      361 gatgcaataa tgaaataagt atacataagt caataataat taataaattt tatgcggtta
      421 tatatgcaaa aatgatatat ccatctatgc cccggtttga ttatgaaaaa aatttatgat
      481 aaactacatg gcagtcttta atgaaaaagg agccaattat gaagggaaat cagaaatacg
      541 taacaacaat tattctttta ctttgctttc tgacacctgc actggtgctg cttttcgcat
      601 tttcagctta cggaatggca cccttcggtg aaaaatccat aatgataatg gacatgtctg
      661 gtcaatattc tgaattctac ataggactgc gtcatttcgg gaaaatgggc aatatgttct
      721 tttcctggtc caaaactctg ggaaccagct atattggcgt ttttgcatac tatctatcaa
      781 gccccttgtc tttcctaacc cttttatcct cagaggccaa catgcccata tttcttctat
      841 ttctgactgt acttaaaatt ggactttgcg gtatgacttt cggaatatac cttatgtata
      901 tgtttaaaag aagagatttt tcagttgttc ttttttcctt gttttatgga ctaatgtcgt
      961 ataacatagt ttactccatg tgtcttatgt ggatagatgg agtcatctgg ctgccaatca
     1021 tcctgattgg aattgaagaa ctgcttaaaa aaggcaagag gaaattgctt gtaacaagct
     1081 tgttttgcgt attcatctcc acctactaca tctcctatat gataggggca ttttcctgca
     1141 tatatcttgt ctacagatat ttatcccact actctgatga gatgcctgta aaaaggcttt
     1201 tcatctattt taccaaactg atttcatcag ctttgattgc cgcacttctt ggtgcatggt
     1261 ttttgattcc aacccttgtt tccctttttg acggaaaaat cggaggagca aactacacgc
     1321 ctcaaggaaa catcaacttt gaactgaaaa accttgtatc aaaactcttt cttggcagtt
     1381 acgacagtat aaccaacagt ggcatgccct ttatttactg tggcattgtg gtaacaatac
     1441 tctttattgg atacttcttt ataaggagcg tatcggtaaa ggaaaaggca tccacatttc
     1501 ttgttgcctc ttacctgctg gcaagcatgt attttattaa tctggatgtt gcatggcaca
     1561 ttttccaatt tcccaactgg tttccctaca gatactcctt cgtcttttta ttcttcatga
     1621 tatttacagc atacaaggca tttgattcaa taaaggattt gtcaaaagcc ttctttgtaa
     1681 cttttcccct ggtttcgata atggtagctc agctggtgtt gaaaataagg tacaggtatt
     1741 tggatgaaga aagaataaca cttactgtaa agctccttgt tttctacatt ttctgcctgc
     1801 tggcaatcaa tctgataaag catgtcaagg aaaaagaagg gttcgacgat tccgtaaaaa
     1861 gatatgccat agcatttcta acattaacaa tgcttgtcat aagcagttac gagctttaca
     1921 aacactccat atacatgtta aaaggacttg acaaggcaca tcattacgag gatattaagc
     1981 cgtatcagga gtacaggaat gctgtaaaca aactccttga agaagcaaaa tcccaaaaag
     2041 ggaagcacga cttttacagg gttacaaaaa acttctcaag aagcatcaac gaaccaataa
     2101 gttttggtta cagtggtatt gatcattaca gctccgccta caacagagta gtcaatgaca
     2161 cattaagaac ccttggattt tcttccgcct atttgtggat taccggatac ggctccacaa
     2221 ttgttacaga cacaatatta aatattgaat accttatgtc aatttcacca gttactaaaa
     2281 gatatgaaaa gcttaaaacc attgaaaaca tatccttgta cagaaaaccg gaagacagaa
     2341 atatttacgg agagcctaaa gacctaagca tatacagaaa cccaattgcc ttgcccgtgg
     2401 ctttcatggc tgattcggag ctattgagaa agaaattgaa agcaccaaat atgtttgcag
     2461 cacaaaacct ttttctttcc catcttgttg gagaaaacat aaactgcttt gaaagaataa
     2521 caccggaaag atacgggaaa tactcttatc ccaaagaaga cgacgattat gtatacatga
     2581 aatcaggcag cgaaggtatc gtatcctact attttattgc agaaaaggac atggagcttt
     2641 atgcttactt tccttttaac aacaatcaca ccaactgctc tgtatatgta aacgacgtat
     2701 atgttgcgaa cctttattac ggtcagtatg actgcgcaca cttcttaggc agcttcaaga
     2761 aaggcgatga ggcaagaatc gaccttatat tcaagtccac atatttaaat acaaggggca
     2821 atatgttcta cgctcttgac tatgacaagt ttgcaagcat ttgcgatgaa tttagagaag
     2881 aaagccttaa agttgactac tttgatggaa atgaaataaa aggcaccgtt tccgcaaagg
     2941 ataacggcac cctgtttaca agcattccct atgacaaggg ttggtcagtt tatgtggacg
     3001 gaaagaaagt aaaaacacac aagtactcgg acactttcct ttgtatagaa gttccgaaag
     3061 gcgaacacga gatcatcttc aagttcacac atgtaggatt tgtaccaggc cttattcttt
     3121 ccataacaac cctattgggt ataattacat tcgaaattct gcaaagaaga aaacttatat
     3181 cgaaatcttg acttccttgt ttgttcttgc agactcgtaa agagcatcaa ttatcttcat
     3241 caacaccaca ccatcctcgg cagggctgat gcatgttttt cctgttctta cacagtcaac
     3301 aaagtggtaa atctctttgt tgaaaatagt accaaaatca aaggcactgg aaccagaggg
     3361 ctctatgtcc accatggtat cgttcaagtc agtgaagata tgaacatagg gatcaatctt
     3421 tgcaccggac tttgtgccaa acaactccac acttcccaca tcatgcttga tgtgcaggtt
     3481 gaagcttgct tcaatgtaca aggtcaagcc attctcaaac tttacaaatc ccgctgcaaa
     3541 gtcttctgta tttgcctcaa aagctccttc cgaatctatc ttccaggctt tgtcaccagc
     3601 aacttccttg tccctcagct tgccgtaaga aacaccatag gcggatacgg gaaggggatt
     3661 tcctgcaaga taccttacca ggtcaattac gtgaactccc aagtcaatca aaggtccgcc
     3721 tcctgaatat ctcttgtcag caaaccatcc gccaggtgtc ccgttccttc ttaaatatga
     3781 agccttagca tagtaaatat ctcccatgtc tcctgcttca ataaatcgct tgattatatc
     3841 cgtgtcgttt ccaaaacgtc tgacaaagcc aatcattaaa agcttgttgt tatcctttgc
     3901 agccttctcc atctccagtg cttcttcctg gttcattgcc ataggctttt cacacaacac
     3961 gttggcgcct gcattcaatg ccgcaatggt agcgttttta tgttcactgt tccatgtgca
     4021 gacactaaca cagtcaagct cctccttttc aagcatttcg ttataatccg tatagtatct
     4081 tggcacattg tatcttgcag catatgcttt taccttttcc tcgtcgatat cgcataccgc
     4141 cacaacatcc acgccttcta tggctttgta tccattcatg tggatgtgac ttatacctcc
     4201 cgtgccaata atacctacat taattttctt cattatgttc cctcctgata atattgctgt
     4261 ctttaattat atacaaaggt cttctcttga cttccagata ggtttttgca agatactgac
     4321 ccagtatccc tattgaaaat aattgaatac cactaagcat aagg
//
LOCUS       DAONVY010000152         4358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14320_length_4358_cov_2.999768, whole genome shotgun sequence.
ACCESSION   DAONVY010000152 DAONVY010000000
VERSION     DAONVY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14320_length_4358_cov_2.999768"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLI11_05215"
     CDS             complement(<1..245)
                     /locus_tag="PLI11_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HPZ52298.1"
                     /translation="MRLFAIILAFISLCLTGCSNTGNSTKKNAGVNTSLKYEKAPVIT
                     ADTEVYNVMDFGAVGDGKKSDTAAIKEAYEYVSNNGGG"
     gene            402..2000
                     /locus_tag="PLI11_05220"
     CDS             402..2000
                     /locus_tag="PLI11_05220"
                     /inference="COORDINATES: protein motif:HMM:NF040163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HPZ52299.1"
                     /translation="MYIKEITAAKDSIVIKYDGDNRSSVYVREKVPTIGLSSREIAFI
                     KAESVDNTITIPRFDGSHDRIWSEFEIWHDSKKAEGIKYVTEFDNVAKYDYPYPQPES
                     IQAAGGTDEDIKFFGTRQNLLNVSLTAIMTPYKGDDTITYVCDGKEYYFIKSEIDNID
                     NTMRRAKRLNLLMTFILLNSPKHFGSKRDPLLLSKVIHPNFDWNDPHAFISSFNMVTE
                     DGQNYYKAFTEFLAERYTNPNSEFGLLGGMIIGNEVDSQYVWGNSGEMTVEDYTLEYT
                     QAMRIAWISARKHYANFRVYVSLDHYWHGLTHNVTMPLRYYDGKSVIDNINKHAKRDG
                     NFDWNVAHHPYPEDLRFPDFYNDRSPEFHFLTRRITFKNIEVLPAYLSQEELLYKGKP
                     RRIILSEQGFNSRGDAISEEQAAAAYCLAYLKITNIPTIDLFTHHAYVDNKYEFGLNL
                     GIRRRNEDGSMGEPKPIYYTIVDLNDETKREERIAKSRAFIGEKLFDSLLNPEVIHGG
                     PILKGNEGFGEPIKKDEKKTYAGM"
     gene            complement(2015..2551)
                     /locus_tag="PLI11_05225"
     CDS             complement(2015..2551)
                     /locus_tag="PLI11_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPZ52300.1"
                     /translation="MYELVKTRRSIRKYLQKEVEKDKIDVIIKSALMAPSSRSRRPWE
                     YVVVTDREKLKELSACREHSAKFLEGAPLGIVVIADSSKSDVWVCDASIASTIIQLTA
                     HSLGLGSCWIQVHNRFDKEGVSAEENVRKALDIPERHSVLCIIAIGYPDESKKPYEEE
                     NLPYEKVHYERYDKEDAE"
     gene            complement(2570..4336)
                     /locus_tag="PLI11_05230"
     CDS             complement(2570..4336)
                     /locus_tag="PLI11_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPZ52301.1"
                     /translation="MEGISIFNIIYLIGGLAIFLFGMTLMGEALERRAGNQLKNVLSK
                     LTTNKYKGFLLGLIVTAIIQSSSATTVMVVGFVNSGIMVLRQAINVIMGANIGTTVTA
                     WLLSLIGIEGDAWFVQILKPTSFTPILALIGCIMYCFINEKKKKHTGLILLGFSVLIF
                     GMDMMSEAVKPLAEVEGFRNILLLFSNPILGVLAGAILTSIIQSSSASVGILQMLSAS
                     GAVTMGSAIPIIMGQNIGTCVTAMISSVGANKNARRAALVHLYFNILGTIVWLTVFSI
                     LNYIFSFAFVNQAATPFTIAIAHTAFNILSTALLFPFTKQLEWLAVRSIPDKKDKSEE
                     IKLLDARLLATPSVAVNRSRTVAVTMANTSVETFKQSMTILKAYDKDLAEKIREGESR
                     VDDLEDMLGSYLVKLSGRNLSEDNSQESYKLLHLLNDFERISDHAVNLLESAEEIHEK
                     QLIFSDEAKKEISVMMEAVNEILDLALRAFVDNDLQAAHSVEPLEEVIDFLKSKLKTQ
                     HIKRLQRNECTIELGFVLTDIVTNLERVADHCSNIAGLVIEVSQNSFDMHNFLKTIKR
                     DPGSEFARLFETYMEKYTIDAD"
BASE COUNT         1335 a          943 c          799 g         1281 t
ORIGIN      
        1 cccccaccat tattcgaaac atattcataa gcctctttta ttgcagcagt atcagacttt
       61 ttcccatcac caactgcccc aaaatccatg acattgtata cttctgtatc agccgtaata
      121 actggtgcct tttcatactt cagcgatgtg ttcacacctg cattcttctt tgtagaattc
      181 cccgtattgc tgcatcctgt aaggcaaagt gatataaaag caagaattat ggcaaataat
      241 ctcatgtttt ctaccctcca catttatgtt aaatacatga tatccgatta aagataattc
      301 atcaatacaa tattacaaat tttgcgtaca atccttattg acacaccgaa atatatgtga
      361 taatagttag acgtccaata agagatgaaa gagggagaga tatgtatatt aaagaaatca
      421 cggcagcaaa ggacagtatc gttatcaaat acgatggcga taaccgctca agtgtttatg
      481 taagagagaa agtgccgaca ataggtctga gcagcagaga aattgctttc ataaaggcag
      541 aaagtgttga taacaccatc actataccca gatttgatgg ctcccatgac aggatttgga
      601 gtgagttcga aatctggcat gacagtaaaa aggcagaggg tatcaaatat gttactgagt
      661 ttgacaatgt ggcaaagtat gattatcctt atccgcaacc tgaatccatt caggctgcag
      721 gcggtacgga tgaggatata aaattttttg gaacccgtca aaatctactt aacgtaagcc
      781 ttaccgccat aatgacacca tataaagggg acgacacaat tacatatgtc tgtgacggca
      841 aggaatatta tttcatcaaa tcagagattg acaatattga taacactatg agaagagcaa
      901 aacgtctcaa tcttttgatg acatttattc ttttgaactc tccgaaacat tttggaagca
      961 aaagggatcc tttgcttcta tcaaaagtta ttcatcccaa ctttgactgg aacgaccccc
     1021 acgcatttat tagcagtttc aacatggtaa ccgaggacgg acaaaactac tacaaggcgt
     1081 ttactgagtt cctggcagaa agatacacaa accccaactc agagttcggt ctcctcggtg
     1141 gtatgattat cggtaacgag gttgacagcc agtatgtatg gggcaactca ggagaaatga
     1201 cagttgaaga ctacactctt gaatacactc aggccatgag aattgcatgg atatccgcaa
     1261 ggaaacacta cgcaaatttt agagtgtatg tttctcttga ccattactgg catggtctta
     1321 cccacaacgt taccatgccg cttcgctact acgatggaaa atccgttata gacaatatta
     1381 acaagcatgc taaaagagat ggcaactttg actggaatgt tgcacatcac ccatatccag
     1441 aagatttaag atttcccgac ttctataacg acaggtctcc agagttccat ttcctgaccc
     1501 gtagaattac attcaagaat attgaagttt tgccagctta cctttcacaa gaagaactct
     1561 tgtacaaagg caaacctaga agaattatcc tatccgagca gggtttcaac tcccgtggag
     1621 atgcaatcag tgaagaacaa gctgcagctg catactgtct tgcatatttg aagataacaa
     1681 atattcctac aattgatttg tttactcacc atgcatatgt agacaataag tatgaatttg
     1741 gtttgaatct aggtatcaga cgcagaaatg aagacggttc catgggtgaa cctaaaccaa
     1801 tctattacac cattgtggat cttaacgacg aaacaaagcg agaggaacgt atagcaaagt
     1861 cacgggcttt catcggagaa aagctgtttg acagtctcct taatccggaa gtaatccatg
     1921 gtggtcctat tctaaaagga aatgaaggct ttggagaacc cataaaaaaa gatgaaaaga
     1981 aaacttatgc aggaatgtaa tacaaaagag gatgtcactc ggcatcctct ttgtcatatc
     2041 tttcataatg tactttttca tagggtaagt tctcttcttc atatggcttt tttgattcat
     2101 cggggtaacc tatagctatt atacacaaga cagaatgtct ctcaggaata tcaagggctt
     2161 tccttacatt ttcttctgca ctcacacctt ctttgtcaaa cctgttatgg acttgtatcc
     2221 aacaagaccc aagaccaagc gaatgcgctg ttagttgaat gattgttgat gcaattgatg
     2281 catcgcaaac ccagacatca ctctttgatg aatccgcaat tacgacaatt cccaaaggag
     2341 cgccttctaa aaactttgcg ctgtgctctc tgcaggcaga aagctctttc agcttctccc
     2401 tgtctgtaac aacaacatat tcccatggtc ttctgcttcg ggaagaagga gccatcaagg
     2461 cacttttgat tattacatct atcttgtcct tctccacttc cttttgcaag tacttgcgta
     2521 tgcttcttct ggtcttcacc agttcataca tagtaagcac ctctctttct taatctgcat
     2581 ctattgtata cttctccatg taggtttcaa acaatcttgc aaactcgctt cctggatctc
     2641 gttttatggt cttaaggaaa ttgtgcatgt caaagctgtt ctgggatact tcaataacca
     2701 atccggcaat gttggaacag tggtccgcta cacgctccag attagttaca atatcagtca
     2761 gcacaaagcc aagttcaatg gtgcactcgt ttctttgcag tctcttgata tgctgagttt
     2821 taagtttcga cttcaggaag tcaataactt cttcaagagg ttccacggaa tgggccgcct
     2881 gcaaatcatt gtcgacaaag gctctcaacg caagatcgag aatctcattg acagcttcca
     2941 tcattacact aatttccttc ttggcttcat ctgaaaagat aagctgtttt tcatgtatct
     3001 cttctgcaga ctcaagcaaa ttgactgcat ggtctgatat ccgttcaaag tcattcagaa
     3061 gatgcaaaag cttgtagctt tcctgactgt tgtcttcgga caagtttctt ccgctaagct
     3121 taaccaaata cgatccaagc atgtcctcta aatcatcaac cctgctctcg ccttccctaa
     3181 ttttttctgc caagtccttg tcgtatgcct taagtattgt catagactgt ttgaatgttt
     3241 caactgatgt atttgccata gtaacagcga cagttctgct tctgttaact gccactgacg
     3301 gagttgcaag caaacgtgcg tcaagtagtt tgatctcttc ggacttatcc tttttgtcag
     3361 ggattgaacg aacagcaagc cactctagtt gttttgtaaa tgggaataac aatgctgtgc
     3421 taagtatatt gaaagctgta tgtgctattg caattgtaaa tggagttgct gcctgattca
     3481 caaaagcaaa tgagaagata tagttcaata tactgaacac cgtcaaccaa acaattgtac
     3541 ctaatatatt gaaatacaga tgtaccaatg cagcacggcg tgcattttta ttcgccccaa
     3601 cagaagatat cattgcagtt acacacgtac caatattctg tcccattata attggtatag
     3661 cagaccccat cgttacggca ccagatgccg ataacatttg gagaatacct acagacgctg
     3721 atgaactttg aataattgat gtcaaaatcg ctcctgcaag cacacctaag attggattgc
     3781 tgaatagaag caggatattt ctaaaacctt ccacttcagc cagcggctta acagcctcac
     3841 tcatcatatc cataccaaaa atcaatacag aaaatcctaa aagaatcaat ccggtatgtt
     3901 tctttttctt ttcattaatg aaacagtaca taatacagcc gattaatgca agaattggtg
     3961 taaaggatgt aggtttaaga atctgtacaa accatgcatc accttcaatt ccaattaacg
     4021 acaaaagcca tgcggttact gttgtaccta tgttggcacc cattatgaca tttatggctt
     4081 gcctcaacac cattattcca gagtttacaa atccaactac cataacagtt gtggctgatg
     4141 aactctggat gatagccgta actattaagc ccaacagaaa acctttgtat ttgtttgtgg
     4201 ttagtttgct tagaacattt tttagctgat tgccggcacg tcgttccaag gcttcaccca
     4261 tcaatgtcat gccgaataag aagattgcta gtccgcctat tagatagata atgttgaata
     4321 ttgatattcc ttccatacca tgtccccttc atatatat
//
LOCUS       DAONVY010000153         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14382_length_4340_cov_3.189032, whole genome shotgun sequence.
ACCESSION   DAONVY010000153 DAONVY010000000
VERSION     DAONVY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14382_length_4340_cov_3.189032"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLI11_05235"
     CDS             <1..1057
                     /locus_tag="PLI11_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPZ52302.1"
                     /translation="IYFPPGRYIVEGTLDLSHQNGNKVVFAGNPDRKKDTFIQSTRSL
                     NADFIILSGSNIHFTYLTIRNLGETGSVVNMQYDNCTLTECAFLQENPKNEEAAIVVS
                     GSNNSISSCYFGPGTTNGYIVNFTKEPGRQSKNNSLTDSYLGGGLPKSVLIDTKDEGH
                     CPEEIFISRNVFLFPAIGQVYVVSVKGLIIHNNMLDAATTAIILNPDLTGISDVDIRY
                     NYMGSQNKDIGMFADRTDMAGGIVTDIANGGTIKNVVVTDNYFWGYYGIRITSSKFNS
                     FKIINNYFVESNGSSIYITESANNVIEGNIIYSGVGAVHTVYIGKIDDKTVFKDNAIA
                     GSISVPDVEKYKDMNMF"
     gene            1070..2218
                     /locus_tag="PLI11_05240"
     CDS             1070..2218
                     /locus_tag="PLI11_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPZ52303.1"
                     /translation="MRRWHFLLILISVLIYASYEKVDGNMNVEKVRFGDVDNRYFNVM
                     DYGAKGDGVGDDTKAIEDAIIAALKVNGTVYFPKGDYIISETIRVEKDDSRRLVLKGD
                     GESSKIIGSDNLKNHMFEILLKYNFQVIDMSFKHNGPGGSCIYSVFLRAFNCYFESNA
                     VNSSPLIDFHGSDCKIDSCTFNTSSKESFSIYYSMLDNEISINNYIVDNMFKGVGKGI
                     LVGDGKYTSSGRCEGLKINNNNFENTGENQIVIQEILHVDIANNIMKGSSMSAIVLAS
                     KGHGPDGIFINHNTISANYACIITEGVKGDYISMTVISDNILSGGSYGIYDNVGLNKC
                     FIRDNVFEKQAKNAIEIEGGVNLFIIDNLIRDKSISVSNSEKYVIKNN"
     gene            2308..2691
                     /locus_tag="PLI11_05245"
     CDS             2308..2691
                     /locus_tag="PLI11_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPZ52304.1"
                     /translation="MKSIKPGRGPSMMGGIVGIAVALFGVFWTIMAVSFGAGFMAVFG
                     IIFVFIAIVQVIYNFKNATSKNRYSSFDIVDSHEEPDPLNERFGTPTRNSSSIKGASS
                     FCPYCGNPVSSEYAFCNKCGKMLPV"
     gene            2712..3239
                     /locus_tag="PLI11_05250"
     CDS             2712..3239
                     /locus_tag="PLI11_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPZ52305.1"
                     /translation="MTIVVISDTHIPGKAKSLPQILYNEIEKSDHIIHAGDVNDIALI
                     NELEIFDPVTVVAGNTDGYQITDKHGFKTIVELSGKRIGVVHGNGPRATFENVRRAFS
                     KDNVDCVVYGHSHIPHCEFLDGILYFNPGSPTDKRLNKYYTYGLIKISEGEFYPEMIY
                     FNKNGIVKRVKVQEE"
     gene            3307..>4340
                     /locus_tag="PLI11_05255"
     CDS             3307..>4340
                     /locus_tag="PLI11_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPZ52306.1"
                     /translation="MFKVSHRCIVLRDDAVERKSLNTKLLGHHLLRVEGIQNAASMYR
                     GKNACIVASGIANIIENFPPIIQDGELIVGYNYPDNPYPDWWICPDTEEYRKLLQEIG
                     FSKERVDRYFATRDKVSELFDYAPNPEFNEVDKQLDTEWASIGRCISDNHSVIGYEDV
                     LKYGFEGLLEKVLEYERKNGSSDLYESMKIICKSACRIGEIYADLARKLQLDPSIDEI
                     RRLELEKIEKVCRQVPAKPARDFWEAVQSLWFVHIINTWEDGINANSLGRLDQILYPY
                     YIHDIETGTYAKDQIFELICCLWIKLYREYDVQQSCVGGCLSDGTSAVNELSYMMLDA
                     TEALGFVRCLS"
BASE COUNT         1468 a          659 c          951 g         1262 t
ORIGIN      
        1 tatatacttt cctcccggaa gatatattgt ggaagggaca ttggacttaa gccatcaaaa
       61 cggaaacaag gttgtttttg caggaaatcc ggatagaaag aaagatacat ttatacaatc
      121 cacaagatca ttaaatgctg attttattat cttgtcaggc tcaaatatcc atttcacata
      181 tttgactatt aggaatcttg gagaaacagg ttctgttgta aacatgcaat atgataactg
      241 tactctcaca gagtgtgcgt ttttacagga aaaccctaaa aacgaggaag ctgccattgt
      301 tgttagcggc tcgaacaact ctatttcttc ctgctacttt ggacctggca ccaccaacgg
      361 ctatattgtc aattttacaa aagagccagg gaggcaatca aaaaacaact cattaacaga
      421 ctcatatctg ggcggaggac ttccaaaatc agtattgatt gatacaaaag atgaaggtca
      481 ttgtccggaa gaaatattta tttcaagaaa tgtatttttg tttcctgcca ttggacaggt
      541 atatgtggtt tctgtaaaag gattaataat acataacaac atgcttgatg ctgcaaccac
      601 cgccattatt ctaaaccctg atttaacagg tatcagtgat gttgatataa ggtacaacta
      661 catgggttcc cagaacaagg atataggcat gtttgcagac agaaccgaca tggcaggtgg
      721 aatagtaacc gatattgcaa acggagggac tattaaaaat gtggttgtaa ctgacaacta
      781 cttttgggga tattacggta tcagaataac ttcaagcaag tttaatagtt ttaaaataat
      841 caacaactat tttgtagaaa gcaatggaag ttcaatttac ataaccgaat ctgcaaacaa
      901 tgtcattgaa ggaaacatca tatacagcgg agttggtgca gtccatacag tatacatagg
      961 aaagattgac gataaaactg tatttaaaga caatgcaata gctggaagca tcagcgtacc
     1021 tgatgtggag aagtacaagg atatgaatat gttctaaggg gtgaacaaga tgagaagatg
     1081 gcattttcta ttgattttga tttctgtttt gatatacgca agctatgaaa aggtggatgg
     1141 taatatgaat gtagagaagg ttcgatttgg ggatgttgat aatagatatt tcaatgtaat
     1201 ggattatggt gccaaaggtg acggtgttgg cgatgacacc aaggcaattg aagacgcaat
     1261 aatagccgca ttgaaagtta acggtaccgt gtattttcct aaaggcgatt atataatatc
     1321 ggaaactata agggttgaga aggatgattc caggagactt gttttaaaag gcgatggaga
     1381 atccagcaaa atcattggta gtgacaacct taaaaatcat atgtttgaaa tactcttaaa
     1441 gtataatttt caggttattg atatgtcatt caagcataat ggacctggtg gaagctgcat
     1501 atattcagtt ttcctaagag cattcaattg ctactttgaa tcaaatgctg tcaattcatc
     1561 gccacttatc gactttcatg gttctgattg caagatagat tcctgtacat tcaataccag
     1621 cagtaaggaa agtttctcca tctattattc aatgctcgac aatgagattt ccatcaacaa
     1681 ctatattgtc gacaatatgt tcaaaggtgt aggaaaaggt attcttgtag gtgatggcaa
     1741 atacacttct tcaggaagat gtgaaggtct taaaatcaac aataacaact ttgaaaacac
     1801 aggcgagaac cagattgtaa ttcaggaaat actgcatgtg gatatagcga acaatataat
     1861 gaaagggtca tcaatgagcg caatagtgct tgcaagtaaa ggccatggac ctgacgggat
     1921 ttttattaat cacaatacca taagcgctaa ttatgcctgt attataactg aaggagtaaa
     1981 aggagactac atatccatga cagtcattag tgacaatata ttgagtggag gaagctacgg
     2041 aatttatgat aatgtaggtt tgaacaaatg ttttattcgg gacaatgtct ttgaaaaaca
     2101 agctaaaaat gcgattgaga ttgaaggtgg ggtcaattta ttcatcattg acaatcttat
     2161 aagagacaaa tccataagtg taagtaacag tgaaaagtat gtgataaaga acaattaaag
     2221 caagtgtcaa gcatcaaata ttgtatcgaa atcatgtgtg tgatatactt atactgtttc
     2281 atataatatg tatatcaagg tggtttaatg aaaagtataa agcctggtag aggaccatcc
     2341 atgatgggtg gaatagtagg aatagctgta gcgttatttg gtgtgttttg gacaataatg
     2401 gcagtcagct ttggtgccgg tttcatggcg gtgtttggca taatatttgt atttattgca
     2461 atagtacagg tgatatataa tttcaagaat gctacgagta aaaacaggta ctcatctttt
     2521 gacattgttg atagtcatga agaaccagat cctttgaatg aaaggtttgg tacgccaaca
     2581 aggaatagtt catcaataaa aggggcgagt tctttttgcc catattgcgg aaatcctgta
     2641 tcttcagaat atgctttttg caataaatgt ggcaaaatgc tgccggtttg attaaaaaag
     2701 gagcaacata tatgacaatt gtggttatat ctgataccca cattccagga aaggcaaaat
     2761 ctttacctca aatcttgtac aacgaaatag aaaagagtga ccatataatc catgctggag
     2821 atgtaaatga tatcgctctg attaatgagc ttgaaatttt tgatccagtt actgtggttg
     2881 caggaaatac cgacggatat caaataacag acaagcacgg atttaaaacg attgttgagt
     2941 tatctggtaa gagaattggc gttgtccatg gcaacggacc aagggcaacc tttgagaatg
     3001 tgagaagggc gttttcaaaa gataatgtgg actgtgttgt atatggacac agccatattc
     3061 cacactgtga gtttttggac ggcattctat actttaaccc cggttcacca actgacaaaa
     3121 gactaaacaa atactataca tatggattga ttaagatttc cgagggtgaa ttttaccctg
     3181 aaatgatata cttcaataag aatggaattg ttaaaagagt aaaagtccag gaagagtagt
     3241 ggaattttaa aatagtatga tatacaatac aagtagttga attgttttgt taaaggagat
     3301 attaagatgt ttaaagtctc gcatagatgt attgtattga gagatgatgc tgtggaaaga
     3361 aaaagcttga acacaaagct tctgggtcat catttgctgc gtgttgaagg aatacaaaat
     3421 gcagccagca tgtacagggg aaaaaatgcc tgcattgttg cgtccggtat tgcaaatata
     3481 atagagaatt ttccacccat aatacaggac ggagagctga ttgttggtta caattatccc
     3541 gacaaccctt atcctgactg gtggatttgc ccggataccg aggaatatcg aaaattactt
     3601 caagaaatcg gattctcaaa agaaagggtt gatagatact ttgctacaag agacaaagta
     3661 tcagaactgt ttgattatgc accaaaccct gagtttaatg aagttgacaa acagctggat
     3721 acggagtggg cttcaatagg tcgctgcata tccgacaatc attcggtaat aggatatgag
     3781 gatgtattaa agtatggatt tgagggcctt ttggaaaagg ttttggaata tgagcgtaag
     3841 aacggctctt ctgatttgta tgaaagcatg aagataatct gtaaatcagc atgcaggatt
     3901 ggtgaaatat atgcagattt ggcaagaaaa ctgcagctgg acccttctat tgatgaaata
     3961 agacgattgg aacttgaaaa aatagaaaag gtatgcagac aggtgcctgc aaaacctgca
     4021 agagacttct gggaagctgt tcagagttta tggtttgtac acattatcaa cacttgggaa
     4081 gacggaataa atgctaactc tttaggcagg ctggatcaga ttctatatcc atattacata
     4141 catgatattg aaacaggaac gtatgcaaaa gatcagatat ttgaacttat ctgttgttta
     4201 tggattaagc tttacaggga gtatgatgtt cagcagtcct gtgttggcgg atgcctgtct
     4261 gatggtacta gtgctgtaaa tgagctgtca tatatgatgc ttgacgcaac agaagcacta
     4321 ggttttgttc gctgcttgtc
//
LOCUS       DAONVY010000154         4322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14462_length_4322_cov_3.572533, whole genome shotgun sequence.
ACCESSION   DAONVY010000154 DAONVY010000000
VERSION     DAONVY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14462_length_4322_cov_3.572533"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="PLI11_05260"
     CDS             complement(<1..155)
                     /locus_tag="PLI11_05260"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPZ52307.1"
                     /translation="MEKVIDLNKTVFELCNEDPAIKDILADSGFTDITKPGMLQTAGR
                     FMTIPKGA"
     gene            complement(157..627)
                     /locus_tag="PLI11_05265"
     CDS             complement(157..627)
                     /locus_tag="PLI11_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPZ52308.1"
                     /translation="MTTSYNDMTKQELAKHIEEKYHAYLRENLPRTSELLYTVLSSHG
                     SDYHVLFEVYSLFGQLRAILEQHLLKEETLLFSKLGHGNNAFAKKEIAIDLMKEHDRT
                     RHILNRLKDITNGYDTTNILCSDFKQAYVMLNTITQDLLNHYYLEETMLLKEFV"
     gene            complement(819..1535)
                     /locus_tag="PLI11_05270"
     CDS             complement(819..1535)
                     /locus_tag="PLI11_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPZ52309.1"
                     /translation="MKSTKGCSYCNHTSCVSKVPIFSSLDERDARDIAALIEHKNYKK
                     GDILINEGDKVNSITIINTGSVKAYKYTPDGREQILYVLTEGDFFGERSLLSSENADF
                     YIEALDDVGVCVITKERFHSLLYTHPDIAINIIDELGKRIATMENAFQSISVRNLDAR
                     IAALLLDYANRYGIMTKDGVKINLPISREGMANQLGIARETMSRKLSQLEKKGIILSE
                     NNKTLIIYNMDMLDEMAGIK"
     gene            1691..2818
                     /locus_tag="PLI11_05275"
     CDS             1691..2818
                     /locus_tag="PLI11_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52310.1"
                     /translation="MVHKRATCIFLIILVLTSLVGISAQEEEREEETNRKVTFSNAQF
                     SVLETYGMERMGAMYMRGFAVSKDGKYLYVGLVHGENGLFRLNTSDGSFDGISYYDKD
                     NGMIKGIATDDRGYVYIGITSETAEGSVSIACLDKDLNELSYEVIEIDGKVGINGVEV
                     AKKGERYYMYFITNYDVNTVRCYDVTDPGNISPAKDVGINGVIYLDKFLGSPIAQANY
                     LKIDEDGSIYLTANMNMFRPKGDTVLKIDKDWKKVQRIFALDDAYGIHLHKDYVFVSR
                     YGEGESNVYILDKNLKVIEVVGWMPGCVNYSGVAVANGRIYIADQLFMGIDRIIVSEV
                     IDDLVEPSRTKQVLTIVLISVGVVVVAIVVYSVKKLQKMLR"
     gene            2867..>4322
                     /locus_tag="PLI11_05280"
     CDS             2867..>4322
                     /locus_tag="PLI11_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52311.1"
                     /translation="MTRRILVILLMLVCVLLTSCTKEKELVMGKMDTYRGYGDGEIII
                     SVFWPPMKGYATEEQYDLLVEAGIDLLEWGTDPIFTDEETLENTLRLCKDKGIKITIC
                     DKDFINLPAKSDEEIRELVRRYKDHDSVVGYYVLDEPRNANPFGRVARIMVEEHPGCI
                     TQLNMLPAFALEDPRGHAEDWINSVGAENLRYLSFDQYPFDLQEGSVPQMFPNFKLIW
                     ETGLKYNVDTALYIQSIGVIGGFRRPTVAETRYHTSAALAYGFKNLKYFTWFTPTERS
                     EEFTEAIVTPDGKKGKTFDGIARINKDIKKVSKILGRVDALEIYHNGRQDLETTMLEK
                     GWHVEAVDDKDFIVSLMKDRFDGRNYIMIVNKNFKDDEKITLKLNGIDKLYDVTSGKA
                     REAKINKGRIKCEIAAGGFRLYALEEGVDLTKPYKDDDEKNLAYNKPVFATSSLGEGG
                     YYVRNAVDGIRIPSQGSKGWRYEGDADSEIYFMVD"
BASE COUNT         1279 a          748 c          978 g         1317 t
ORIGIN      
        1 gctccttttg gtattgtcat gaacctgccg gcggtttgaa gcattcccgg ttttgttatg
       61 tcggtaaaac ctgaatccgc cagtatgtct tttattgcag gatcctcatt gcataattca
      121 aatacggttt tattcaaatc aataactttt tccattttaa acaaactcct ttaacagcat
      181 ggtttcttca aggtaataat gatttaacaa atcctgcgtt attgtgttca acattacata
      241 tgcctgcttg aagtcagaac atagtatgtt ggtagtatca tagccattgg ttatatcctt
      301 gagcctgttg agaatatgcc ttgttctgtc gtgttctttc atgaggtcta ttgcaatttc
      361 tttctttgca aaagcattgt tcccatggcc cagcttgctg aagagcagtg tttcctcttt
      421 cagcaaatgc tgctcaagga ttgctctcaa ttgaccaaac aggctgtata cttcaaacag
      481 tacatggtaa tcactgccgt ggctgcttag gacagtgtaa agaagttccg atgtcctggg
      541 gaggttctcc ctaaggtatg cgtggtattt ctcttcaata tgttttgcaa gttcctgttt
      601 ggtcatgtca ttgtacgaag ttgtcatgct ccatcccctt tacattttct acagttttat
      661 tctacccggt aaaacgaaac ctgtatgtga tttgaatcac ctggcgttaa cttgtggggt
      721 gaagcatacc attaatttat taaataattt gtcatgtaca atgaggaatg ttaaaataaa
      781 tttgtgatta tattaattaa aaataaatca gcggagcatt attttatccc cgccatttca
      841 tccagcatat ccatgttgta aattatcaaa gttttattat tttctgaaag aattatacct
      901 tttttctcaa gctggctcag cttcctgctc attgtttctc ttgcaatacc aagctgatta
      961 gccatgcctt ctctgcttat gggaagattt attttaaccc cgtcttttgt cattatgccg
     1021 tacctgtttg cgtaatccaa taaaagtgct gcaatgcgtg catcgaggtt tctcacacta
     1081 atgctttgga atgcgttttc cattgttgca atccttttac ccagttcatc tattatgttt
     1141 atggcgatgt ctggatgggt gtaaagtagg ctgtggaacc tttcctttgt tatcacgcat
     1201 actcccacat catcaagagc ttctatgtaa aaatcggcat tttcagagct taacaggctg
     1261 cgttctccga aaaagtcacc ttcagtcagt acgtaaagta tctgttccct cccgtctgga
     1321 gtatatttat atgcctttac actaccggtg ttaatgatgg ttatggagtt taccttgtcg
     1381 ccctcattga ttagaatgtc gcctttttta tagtttttgt gttctatcaa tgctgcgatg
     1441 tctcttgcat ccctttcatc aagagaggaa aagatgggta ccttgcttac acagcttgtg
     1501 tgattgcagt atgaacatcc tttggttgat ttcattttat ccctccataa gcaatgtata
     1561 aagatatgat aacattagga atacaggtta tgcaacttta tttatatgtg cttgcagacc
     1621 gaattgattg aaaaacctgt caggacgtat tataataccc gtgttagatt atcaaaagag
     1681 aggtattatc gtggtacata aaagagctac atgcatattt ttaatcatac ttgttctgac
     1741 aagtcttgtt ggcatatcag cccaggagga agagcgcgaa gaagaaacca acagaaaagt
     1801 aactttttcc aacgcacagt tttcagtcct tgaaacttac ggcatggaaa gaatgggagc
     1861 catgtacatg agggggtttg ccgtttctaa agatggcaaa tacctttatg taggtcttgt
     1921 ccatggagaa aatggcttgt tccgtttgaa cacttctgat ggaagttttg acggcataag
     1981 ttactatgac aaggacaacg gaatgataaa aggcattgct acagatgaca gaggttatgt
     2041 gtacatagga ataaccagtg aaactgcaga aggaagtgtc agcattgcct gtcttgacaa
     2101 ggatttgaat gagttgtcct atgaggtcat agagattgat ggcaaggtag gcattaacgg
     2161 tgtggaagtt gccaaaaagg gcgagagata ttacatgtac ttcataacca actacgatgt
     2221 gaataccgtt agatgctatg atgtaacaga ccctggcaat atttctccgg caaaggatgt
     2281 tggaataaat ggtgtgattt atctcgacaa gtttttggga agtcctattg cgcaggcaaa
     2341 ttacctgaag attgatgagg acggcagcat atacttaact gcaaacatga acatgtttcg
     2401 cccaaaaggg gatacggttc ttaaaattga taaggattgg aaaaaggttc agaggatatt
     2461 tgcccttgac gatgcatatg gaatacattt gcacaaggat tatgtatttg tttcaagata
     2521 tggcgaaggc gaatccaatg tgtatatact ggacaaaaat ctgaaagtga ttgaggttgt
     2581 tggttggatg ccaggatgtg taaattactc aggtgttgct gttgcaaacg gaaggattta
     2641 catagcggat cagctcttca tgggaattga cagaataatt gtttcagaag taattgatga
     2701 ccttgtagaa ccttcacgta ccaaacaagt tctaactata gtcttaatat cagttggcgt
     2761 ggttgttgtg gcaatagttg tttactccgt caagaaactt cagaagatgc tgagataaaa
     2821 atatggtata ctaatcatta gtatacaaaa agcaggtgca tgtaaaatga caagaagaat
     2881 tctagtaata ctattgatgc ttgtatgcgt tttattgaca tcttgcacaa aagaaaagga
     2941 gttggtgatg ggaaaaatgg atacatacag aggatacggg gacggcgaga taataatttc
     3001 tgtattctgg ccgcccatga aaggctatgc cacggaagag cagtatgatt tgctggtgga
     3061 ggctggaatt gatcttttgg aatggggaac cgacccaata ttcacagacg aggaaacgct
     3121 tgagaataca ctaagattat gcaaggataa gggtataaaa attaccatat gcgacaagga
     3181 ttttattaat cttcctgcca agagtgacga agagataagg gagctggtaa gaaggtataa
     3241 ggatcatgat agcgttgttg gttactatgt tcttgatgag ccccgcaatg caaatccttt
     3301 tggcagggtg gccagaatca tggtagagga gcatcctggc tgcataaccc agcttaacat
     3361 gctgcctgcg tttgctcttg aagacccaag aggacatgca gaggactgga taaattctgt
     3421 aggtgctgag aatttgaggt acctgtcttt tgaccaatat ccttttgacc ttcaggaagg
     3481 ttccgtccca cagatgtttc caaacttcaa actgatttgg gaaacgggtc ttaaatacaa
     3541 tgtggatacc gccttgtaca tacagtcaat tggtgtaatt ggtggtttta gaaggcctac
     3601 agttgcagaa actagatacc atacatctgc tgctttggct tatggtttta agaatttaaa
     3661 gtattttacc tggtttactc ctactgaaag aagtgaggag tttactgaag caattgtaac
     3721 gccggacgga aagaaaggta aaacctttga tggtattgcc aggataaaca aggatattaa
     3781 aaaggtatca aagattcttg gaagagtgga tgctctagag atatatcata atggcagaca
     3841 ggatttggag accacaatgc tggaaaaagg ctggcatgtt gaggctgtcg acgacaagga
     3901 ctttatagtc tctcttatga aagacagatt tgacggcaga aactatatca tgattgtaaa
     3961 caagaacttc aaggatgatg agaaaataac attaaaattg aatggtatag acaaacttta
     4021 cgatgttacc agcggaaaag caagggaagc gaagataaac aagggcagaa tcaagtgtga
     4081 gattgctgcg gggggcttca gactgtatgc ccttgaagaa ggcgtggatt tgacaaagcc
     4141 atacaaggat gatgatgaaa agaatcttgc atacaacaaa ccagtgtttg ccacctcttc
     4201 tcttggagaa ggcggatact atgtccgcaa tgcggtggat ggtataagga taccgtcgca
     4261 aggctcaaaa gggtggaggt atgaaggaga tgctgatagt gaaatatatt ttatggtgga
     4321 tt
//
LOCUS       DAONVY010000155         4282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14617_length_4282_cov_3.845517, whole genome shotgun sequence.
ACCESSION   DAONVY010000155 DAONVY010000000
VERSION     DAONVY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14617_length_4282_cov_3.845517"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLI11_05285"
     CDS             <1..660
                     /locus_tag="PLI11_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPZ52312.1"
                     /translation="SQDAGAARTFIISEPLAAAIGAGIDISKPTGNMIVDVGGGTTDI
                     AVISYGGIVVNKSVKVAGDALDETIKNYLKKTHNLLIGDRTAEQIKVEIGCVYHGIRN
                     ESLEIRGSDLITGLPNERIITSEEILEPLMEAVIPILDGIHAVLEETPPELAADIYSR
                     GIILTGGGAMIAGLDELIRKSTGIDAIIADEPQYCVAKGCNMFIMSMSSKEKIEAGKI
                     S"
     gene            complement(757..1812)
                     /locus_tag="PLI11_05290"
     CDS             complement(757..1812)
                     /locus_tag="PLI11_05290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPZ52313.1"
                     /translation="MTHSVVCYNDNEYSGWPANGGIWSWGSEILVSFNRGRFQENNLF
                     HRVIESDMQCVFSRSVDGGLTWAEVDFDNSIYEKELKQVPEEGFEFDENFVMRVGRPA
                     ITIKGDVYIVSCDKGKTWDGPFEFPSFGRENTSRTSYLIERNKKMRVFMSHNVLENPI
                     NKPFYDRAFVAITEDGCKSWSFVGDITDDAPRSVMPSVVRLDDNTLVAALRRRMKRPP
                     MDDVWIEVKRSNDNGRTWHTPVRAADTYNINNAANTNGNPPAMCKLSDGTLVLAYGRR
                     EPGNSSLRVCISTDGGLTFHSDRAIRQDAIDMDMGYPRIVARPDDKCVVVYYIATKER
                     PVQHIEATIFDPMSLNI"
     gene            complement(1899..3317)
                     /gene="murC"
                     /locus_tag="PLI11_05295"
     CDS             complement(1899..3317)
                     /gene="murC"
                     /locus_tag="PLI11_05295"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPZ52314.1"
                     /translation="MNLTGNHIERENPSRESDLNLPAGCRIHFIGIGGSSMSGLAEIV
                     LERGFRVSGSDRVESCNTQKLRQKGAKLYIGHDALNVPDDTYLVVYTLAISKDNPEYL
                     KAIEKGIPVMERGHFLGLLTREHDYSICIAGTHGKTTTTSMLAHVMLHANLDPSVHIG
                     GNLPVLGGNVRSSKSPYFVTEACEYHENFLNLSPYAGVILNIEAEHLDYFKDIDDIIY
                     AFKKFIKLIPKEGFAVVCAESKPALFAAEAADCKVYTYGFSDDCDFIAKDIQYKENET
                     RFKVYNNDGILTDLVLKVPGKHNVLNALATCAVCINLGCEKEAVKEGLLNFVSPDRRF
                     QNRGFINGAPIIDDYAHHPTEIKATLDSAKQITKGRVLCVFQPHTYSRAKAFGDDFAE
                     ALLDADNLVVTDIYAAREKNPGDINSQMLAEKFLSKGINTKYISDFKEIAEYLKKEVR
                     ENDTILLLGAGNVNDIVSFLNE"
     gene            3530..>4282
                     /locus_tag="PLI11_05300"
     CDS             3530..>4282
                     /locus_tag="PLI11_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52315.1"
                     /translation="MLKLVNVTKSYVTGDFKQVALNNVSLAFRENEFVAILGPSGGGK
                     TTLLNIIGGLDRYDSGEMYINGRPTSDFKGVEWDAYRNNSIGFVFQNYNLISHISILE
                     NVEMGMTLSGMSASERRERALRLLDRVGLKDHVKKKPNQLSGGQMQRVAIARALANDP
                     DIILADEPTGALDSETGVQILDLIKEIAKNKLVIMVTHNPELANKYATRIVNLVDGRI
                     VSDSNPYEEEEHKQSGYKLKMTAMSFFTALKLS"
BASE COUNT         1218 a          887 c          897 g         1280 t
ORIGIN      
        1 tcccaagatg ctggtgctgc aagaaccttt attattagcg agccattggc tgctgcaata
       61 ggtgccggaa tagatatttc aaagcctaca ggaaacatga ttgtagacgt tgggggagga
      121 accacggata ttgcagtaat ctcttacggt ggcattgtag taaacaaaag tgtaaaagtt
      181 gctggagatg ccctggatga aaccataaag aactatttga agaaaactca caatctgctt
      241 ataggtgaca ggactgcaga acaaataaag gtggagatag gatgcgttta ccatggaata
      301 cgcaatgaat ccttggagat tagaggcagt gaccttataa caggtcttcc aaacgaaaga
      361 ataattactt ccgaagaaat ccttgagcct ttgatggagg cggtaattcc aattcttgat
      421 ggaattcacg cagtgcttga agagacacct cctgagcttg ctgctgacat atacagtaga
      481 ggcataatcc ttacaggagg gggcgcaatg atagcaggtc ttgatgagtt aatcagaaag
      541 agtaccggta tagatgcgat aattgcggac gagcctcagt actgtgtggc aaaaggatgc
      601 aacatgttta ttatgtccat gtcatctaaa gagaaaattg aagcgggtaa gattagctaa
      661 aaagatagca attacggcag gtattattac ctgcctcttt ttttgtcttt taggtaaata
      721 attggtctga ttgaagtctt taaccgggtg cttcaattat atgtttaagc tcatagggtc
      781 aaatatcgtc gcctcaatat gttgcacggg tctttctttt gtagctatgt aatataccac
      841 tacacatttg tcatccggtc ttgcaacaat tcttggatat cccatatcca tgtctatggc
      901 atcttgcctg atggccctgt cactgtgaaa agttaaacct ccgtctgttg atatgcaaac
      961 tcttaaagaa gagttaccag gctcccgcct tccgtatgca agaaccaggg tgccgtccga
     1021 aagtttgcac attgcaggcg gattgccatt tgtgttggct gcattgttta tgttatacgt
     1081 atccgctgcg cgaaccggag tgtgccatgt cctgccattg tcgttcgatc ttttaacctc
     1141 aatccaaaca tcatccatgg gcggtctttt cattcttctt cttagtgcgg cgaccaatgt
     1201 gttgtcgtca agacggacca cacttggcat aaccgatctt ggcgcatcat ctgtaatatc
     1261 ccctacaaaa ctccagcttt tacagccatc ttctgttatg gcaacaaaag cacggtcgta
     1321 aaagggtttg tttattgggt tttccaatac attgtgtgac ataaagaccc gcatcttttt
     1381 gtttctttct atcagatagc tggttcttga ggtgttttct ctgccaaacg aaggaaattc
     1441 aaaaggacca tcccaggttt tgcctttgtc gcatgaaact atgtacacat ctcccttgat
     1501 tgttatggct ggtcggccta ctctcattac aaagttttca tcaaactcaa aaccttcttc
     1561 gggcacctgt tttaattctt tttcataaat tgagttgtca aaatcaactt ctgcccatgt
     1621 cagtccgcca tctacactgc gagaaaacac gcactgcata tctgactcta taacccgatg
     1681 aaatagattg ttttcttgaa atcttcctct attgaaggaa acaagtatct cgcttcccca
     1741 actccagata cctccgtttg ccggccaacc tgaatactcg ttatcgttgt agcaaaccac
     1801 actgtgtgtc atgaaacaac ccctccaatt ctacattgag ttataaaatt actaaattta
     1861 atggcaggga caaataccct gccataccaa gtaggtgttt attcatttaa aaacgatact
     1921 atatcattca cattaccagc gcctagaagc aggatagtgt cgttttctct tacttctttc
     1981 tttaaatact cagctatttc cttaaaatcg ctgatgtatt ttgtattaat accttttgag
     2041 agaaatttct ctgcaagcat ttgcgagttt atgtctcccg ggtttttctc ccttgcagca
     2101 taaatatctg ttacaaccag gttatctgca tctagtaaag cctctgcaaa gtcgtccccg
     2161 aaagcttttg ctctggaata tgtatggggc tggaatacgc aaaggactct tcctttggta
     2221 atttgttttg cggaatcaag agtcgcctta atctcagtag gatgatgggc gtagtcgtca
     2281 ataattggcg caccgtttat aaatccgcga ttttgaaaac gtctgtcggg tgaaacgaaa
     2341 ttcagaagcc cttcttttac tgcttctttt tcgcagccta ggtttatgca aactgcgcag
     2401 gttgccagag cattcagaac attatgtttg ccaggtactt tcaataccag gtcagtaaga
     2461 attccgtcat tgttatacac cttgaaacga gtttcgtttt ccttgtactg tatgtctttt
     2521 gctatgaagt cgcaatcgtc ggaaaatcca tatgtataga ccttgcagtc tgctgcttct
     2581 gctgcaaaca acgcaggttt gctttccgca caaacaaccg caaagccttc tttgggaata
     2641 agtttaataa attttttaaa cgcatatatt atatcatcta tatccttgaa atagtctaga
     2701 tgttctgctt caatgtttag aataacgcct gcataaggag aaaggtttaa aaaattctca
     2761 tgatattcac aggcttccgt aacaaagtac gggctttttg atgatctgac atttcctcca
     2821 agaacaggta agttcccgcc aatatgtacg ctgggatcaa gatttgcgtg gagcatgaca
     2881 tgagccagca ttgaagtagt agttgttttt ccatgagttc ctgcaatgca aatggagtaa
     2941 tcgtgttccc ttgttaaaag gccgagaaaa tggcctcttt ccataaccgg gatgcccttt
     3001 tcaatggctt tcaaatattc gggattgtct ttcgaaattg caagagtata aacaaccaga
     3061 taggtgtcgt cggggacatt gagcgcatcg tgccctatat ataatttggc acctttttgt
     3121 ctcagttttt gtgtattgca ggattcgact ctatcggaac cgcttacacg aaagcctcgt
     3181 tcaagtacga tctcagccag accgctcatg ctggagccgc ctatccctat gaaatgtatt
     3241 ctacatcctg ctggcaggtt gagatcagat tccctggaag ggttctcgcg ctctatatga
     3301 ttgcctgtca agttcattta tcagatcacc gcatccttta tcacactaaa ttgatacata
     3361 aatatattat tccttgaatt atcaaattgt ccggaatcag aacaattata tcacatatgt
     3421 tcgtttattg ctgaatattt tttgaccatg gtataaaaaa acagtataat tcgaaatatg
     3481 tattttaaaa tgctgtatta gtaaaatata ttgagaaggt agagggttta tgttaaaact
     3541 tgtaaatgtt acaaaaagtt atgttaccgg cgatttcaag caggttgctt taaataatgt
     3601 gagtcttgca ttcagggaaa atgagtttgt tgcaattctg ggtccttcag gaggcggaaa
     3661 gacgacgcta ttaaacatca taggcggcct ggacagatat gacagtggcg agatgtatat
     3721 aaacggcaga cccacatccg acttcaaggg tgtggagtgg gacgcttata ggaacaacag
     3781 catcggcttt gttttccaga attacaacct tatttcccat atatctatcc ttgaaaatgt
     3841 agagatggga atgactcttt ctggaatgtc tgccagcgaa cgaagggaaa gggcccttcg
     3901 attgcttgac agggtaggtc ttaaagacca tgtcaaaaag aaacccaacc agctgtcagg
     3961 cggacagatg cagagagttg cgatagcaag agcgctcgca aatgatcctg atattattct
     4021 ggcggatgag ccaactggag ctttggatag cgaaacagga gtacaaatac ttgaccttat
     4081 aaaagagatt gcgaaaaaca aacttgttat aatggttaca cacaatcccg aactggcaaa
     4141 caagtatgcc accagaattg tcaatttggt ggacggacgg attgtaagcg actccaaccc
     4201 gtatgaggag gaagaacaca agcagtcagg atacaaactc aaaatgactg ccatgagctt
     4261 tttcaccgcg ctgaagctat cc
//
LOCUS       DAONVY010000156         4279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14639_length_4279_cov_2.556345, whole genome shotgun sequence.
ACCESSION   DAONVY010000156 DAONVY010000000
VERSION     DAONVY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14639_length_4279_cov_2.556345"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="PLI11_05305"
     CDS             complement(<1..1960)
                     /locus_tag="PLI11_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52316.1"
                     /translation="MKAVKACLIIISAMLGFGIIVANGASEKVALFDKEKLVNEMRLY
                     SHDNLAIQFNATEDFDEVTIEVNAGKHGKGKLTVSIYEWDEFYAKTLRKEPVRKKSFE
                     GFGNKITYKFDTLPKGEYILLLNKASGKAVNIKIVEKSINNVRVYYNDRAEPSIGVAA
                     YVRFISGNGKKLGEISENKSDEPILEFDYFNPVDDREYKVRPDTWAAVDGLGRILPAY
                     KDVGNLKDKKVGIFYTIWFEEEYSNSITPYSLQKLMTEYPDARLDPIHRLWSTIESQS
                     RVIYYWNEPLYGYYSGTDAFVYRKHAELLADAGVDFIVFDCTNATYLWRAQFLCLVDA
                     FMKAKSEGVKVPQFAFFLNSRSQYELSTQLDMLYRDIFARGLYQDLWFYLDGKPLIMA
                     NKNILDNEKHKYLLDSFTFRANSGDYADKHNEEIGYWQWLRIHPQGRFGVRKDGSVEY
                     VPVSVAQNGNRELPGFSAMNQTDPKLRPTGRSWTYKNGWIGDDDFTEGNIISKTKLQG
                     LNFQEQWDYAHEVDPDIVFIVGWNEWTVHRYAEFDTGHVKLINAFVDQFDDENSRDIE
                     MTKGDLKDHYYYQMVSNIRKFKGMSVPDAVNAYKKIDILKGVEQWKDVYPEYHHYTGS
                     TPYREAGGFRGAVFDANDTMRNDFVLAKV"
     gene            complement(1957..2934)
                     /locus_tag="PLI11_05310"
                     /pseudo
     CDS             complement(1957..2934)
                     /locus_tag="PLI11_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
     gene            3218..>4279
                     /locus_tag="PLI11_05315"
     CDS             3218..>4279
                     /locus_tag="PLI11_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52317.1"
                     /translation="MILNKNLAGYLQPLTEEELIYKEYYELKQKNAPLEEFFESIANR
                     NDIRKDLFIKELEGSGYYEKVTDIFYPFDYDIVAARHTRYHPLFLHRHPFYEIVYQIE
                     GTCTNTINNITVEMTEGDICIIPPGPVHTVGVFDNSIIVNILVKPESITNSLHELLAR
                     NNIITKFFEGNEFTYSYNYALFRVTNDIELKDIMESLILECFNKDIHTLPMRKTLLML
                     AFNHLLRYQLNNMELSENRNKLHTKVQSICQYLKTSYQTTNLHDTARQFNYSPTYLCK
                     LIRNSTGHTFNDILTRIRITKACDLLATTKKGINDIAELVGYESPEYFNKVFKKYMGV
                     SATEYRKDYNMSVIKNMQAT"
BASE COUNT         1294 a          966 c          696 g         1323 t
ORIGIN      
        1 caacttttgc aaggacaaaa tcattcctca tggtatcatt ggcatcaaac acagcacctc
       61 taaagccacc tgcttcccta tatggcgtgg agccagtgta gtgatggtac tcaggataaa
      121 catccttcca ctgttccact cctttcaata tgtcaatctt tttgtatgca ttaaccgcat
      181 caggcacgct cattccttta aactttctaa tattgcttac catttgatag taatagtggt
      241 ctttcaagtc accttttgtc atttctatat cacgactgtt ttcatcatca aattgatcaa
      301 caaaggcatt aatcaatttc acatggcctg tgtcaaattc agcatatcta tgtacggtcc
      361 actcattcca acccacaatg aatacaatat cagggtcaac ctcatgcgca taatcccact
      421 gttcctgaaa attcagacct tgtagttttg tcttagagat gatgttgcct tcagtaaaat
      481 catcatctcc aatccatcca tttttatatg tccagcttct acctgtaggt cttagtttcg
      541 ggtcagtctg attcatagca gaaaaaccag gtaactccct gttgccattt tgggcaacac
      601 ttactggcac atactctaca gagccgtcct tccttactcc aaatctaccc tgtggatgaa
      661 ttctcagcca ctgccagtat cctatctctt cattatgctt gtctgcataa tcgccactgt
      721 ttgccctgaa tgtaaacgaa tcaagaagat atttgtgttt ttcattatcc aatatgttct
      781 tgtttgccat gattaatggt tttccgtcca aataaaacca tagatcctgg tacaacccac
      841 gagcgaatat gtctctgtac aacatgtcaa gctgtgtact taattcatat tggctccttg
      901 agtttaaaaa gaatgcaaac tgcggaactt taactccttc cgacttggcc ttcatgaatg
      961 catccacaag gcacagaaac tgtgctctcc agagatacgt tgcgtttgtg cagtcaaata
     1021 caatgaagtc cacacctgca tcagcaagaa gttctgcatg ctttctataa acaaaagcat
     1081 ctgttccgga gtagtatccg tacaaaggct cattccagta atatatcact ctgctttggg
     1141 actcaatagt cgaccacagt ctatgaattg gatcaagtct tgcatccggg tattctgtca
     1201 tcaacttctg caggctgtat ggggttattg aattggaata ctcttcctca aaccatattg
     1261 tgtagaatat gccaaccttt ttatccttta agttacccac atccttgtag gcaggaagta
     1321 ttcttccaag cccgtctact gcagcccagg tatcaggtct gaccttgtac tctctatcat
     1381 caacaggatt gaaatagtca aactcaagta tgggctcgtc actcttgttc tccgatatct
     1441 caccaagttt ctttccattg ccgctaataa atcttacata cgcagcaaca ccaatagaag
     1501 gctctgccct gtcattgtaa tagactctta cattattgat actcttctcc actattttga
     1561 tatttaccgc tttaccagat gccttgttta aaagaagtat gtactcacct tttggcaggg
     1621 tgtcgaactt gtaagttatt ttattgccaa aaccttcaaa tgacttcttg cgaacaggtt
     1681 cctttctcaa tgttttggca taaaactcat cccactcata aattgacact gtaagttttc
     1741 ccttgccgtg ctttccagca ttaacctcta tggttacttc gtcaaaatcc tccgttgcat
     1801 taaactgtat agccaggttg tcatgggaat acaacctcat ttcatttacc agtttctcct
     1861 tatcaaacaa ggcaactttt tcagacgcac catttgccac tattatccca aaacccagca
     1921 ttgctgaaat aataattaga catgctttta cagccttcat tgccaacctc cgttcattct
     1981 tgccaaatct ttcaaaacct gcattgcagg tgctggaaac cttcccaaat gatcaggccc
     2041 aatgttcaat aaataatttg caccaatcct tttaagatgt tcttttgttt taaacacttc
     2101 ctctgcactt ttccagttgt tatcgtaata tttaaaaccc caggaatcat tcaaggttgc
     2161 tggactttct tacaaaacat ccaaaacctt atctataggt atctcattgt ctcctgctga
     2221 tatatagtcg taaatgccat tgccaagtct agagtttaca agacaatctg gctgataatg
     2281 ttttaccatg ctgtatagtt caagactttt ttctttcgat attacaagtg gtgtatcaaa
     2341 ccagatcagg aacagctctc catagtttgt gaggatttct ttaagctgag gctttatctt
     2401 ttcttcatag caaatatcgt agcatttgct ttcattatca ggaaaatccc agtcattcgt
     2461 ccaggaagta gtcccttcaa gatttaaaaa aggatatctg tacccaccgc catgcggatg
     2521 ggaccaatcg atatcctgtg aatagtaaag accgagttta atgccgtgtt tgtaacaggc
     2581 ttcagcaagt tcacctacaa catctcgtcc aaaaggtgtc atgtccacca cattgaattt
     2641 tgttgctttg gaatgaaaca tggcaaatcc atcatggtgt tttgaagtga agaccatgta
     2701 cttcataccc gctgcttttg caagtcttgt aaactcttct gcattaaaat atattggatt
     2761 gaacgctttt gcaagttcat ggtattcctt gttaggtatg cggaaatatg cctgaatcca
     2821 ttctgcgata tacggcattc tttgcccttt ccactcccca cccaataaag aatacaatcc
     2881 aaaatgaatc ataagaccaa actgcgcttc cttaaaacgt aacctgacat ccataaaagc
     2941 acctatcttt cgcaccttgt atactgttag taattatatt gcttgtagga aaatattgca
     3001 attagaaaag caaaaagcgg acctttcagc ccgcttaagc ttttctaact tatttgtttt
     3061 ttataaccca gtctatacaa ttataaatta cattgcttta tattacaggt tcattcttgt
     3121 caatttgcca ctttttcggt cttttcttgt tgtttcagct taatttatta ttgaaccaac
     3181 ctggttaatc tgtataataa tatcaggagt gatgaagttg attttaaaca aaaacctagc
     3241 aggatacctg cagccattaa ctgaagaaga attaatatac aaagaatatt atgaacttaa
     3301 gcaaaagaac gcacctcttg aggaattctt tgaatccatt gcaaacagga atgatataag
     3361 gaaagacttg ttcataaaag aattggaagg ctccggatac tacgaaaagg taacggatat
     3421 attctatcct ttcgactacg acatagttgc agcaagacac acaagatatc atcccctatt
     3481 cttacacaga catccttttt atgagattgt ctatcagatt gagggtacct gcacaaatac
     3541 aataaacaac ataacagtag agatgacgga aggggacata tgtataatac ctccaggacc
     3601 tgttcatacc gttggtgttt ttgacaacag cataatagtt aacatacttg taaaaccgga
     3661 atcaatcaca aactcgttac acgaacttct tgcaagaaac aatattataa ccaagttctt
     3721 tgagggtaat gagttcacat actcttacaa ctatgcactg ttcagggtaa caaacgacat
     3781 agagttgaaa gacataatgg agtcgctgat acttgaatgt ttcaacaagg atattcatac
     3841 gctgcccatg agaaagactc ttctaatgct ggcattcaat cacttgctga gataccagct
     3901 taacaacatg gagctttccg agaacagaaa caagctgcac acaaaggttc aaagcatatg
     3961 ccagtatctc aaaacaagtt accagactac aaatctgcac gataccgcaa ggcagttcaa
     4021 ttactctccc acatatcttt gcaaattaat tagaaacagt accggacata ccttcaacga
     4081 catattaacc aggataagaa taaccaaagc ttgcgatctc cttgccacaa caaaaaaggg
     4141 cattaacgat attgcagaac ttgtaggata cgaatctcct gaatacttca acaaagtctt
     4201 caaaaaatac atgggtgtgt ccgcaacaga atacaggaag gattacaata tgtcggttat
     4261 caagaatatg caggcaaca
//
LOCUS       DAONVY010000157         4274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14662_length_4274_cov_4.626926, whole genome shotgun sequence.
ACCESSION   DAONVY010000157 DAONVY010000000
VERSION     DAONVY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14662_length_4274_cov_4.626926"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1351)
                     /locus_tag="PLI11_05320"
     CDS             complement(2..1351)
                     /locus_tag="PLI11_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPZ52318.1"
                     /translation="MQIFYTVRKGDTLYNIASRWQVPVESLVYMNHIREPNLLQIGSQ
                     LSMPMGITSYVVKPGDTLFKISSFYGISLDTLINANNISPPYTIYPDQVLTVPAGVPW
                     YTVRKGDTLFSIARRYNTSSSLIFEANNLETPIIHPGTNLKIPFSPDESFNNLVLIIF
                     DDINHFLIIYNGFTGGFESIKVPDGGDGARIFVSPNGKHISYVSNEGIIYVIDKDTEE
                     VSSIDKIDVPGFVSWSKHGNRIVYSNSSVIRLYNVLTRQFETIERPLARYVQFLDEAS
                     LLFESGMSIYKLNLIDNTEERVFQFSNPINDVLASPDGSWFLFTSPGASISEIYTVNL
                     NDGSLNKLPSGQQAKNFNPVWSHDSSRIAYSETVFENGRYYSIIRITEPVGNLARKVA
                     ISNSYATPLVWSKNSQNVAYMSGRHDLSVFTEVWSVNTSLDVPKSILTGFTFIDFDM"
     gene            1436..2680
                     /locus_tag="PLI11_05325"
     CDS             1436..2680
                     /locus_tag="PLI11_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPZ52319.1"
                     /translation="MKYINDIWTRLTESKVLVAVLVQGLKVVSILAVCIIAYLITTKV
                     VLKLLSMYINKTKSSWDDAFLKRKVFTKLARLVPAIIIYVSAPIIPEIRVVIERAALS
                     FVYLNVAFVLSAVLDAANDIYMTFPVSKARPIKGFLQIIKIVVYIIMGVLVISVILDK
                     SPWILLSSIGALTAITTLVFKDSIMGFVAGVQLTTNDMLRIGDWIEMPKYGADGDVIE
                     ITLNTVKVRNWDKTITTIPAYALVSDSFKNWRGMHETGGRRIKRAIYIDVTSIKFCTP
                     EMLERFKKIQLLTEYIENKEKEIEEYNEEHAIDTSLLVNGRHLTNIGTFRKYIEYYLE
                     NHPRVHKGLIRMVRQLSPDDRGLPLEIYAFVDNTQWVTYENVQADIFDHIFSVVDTFE
                     LRIFQKPSGNDMQLLGSSLKDS"
     gene            complement(2677..3174)
                     /locus_tag="PLI11_05330"
     CDS             complement(2677..3174)
                     /locus_tag="PLI11_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52320.1"
                     /translation="MKRIINLILIISTLSSLFVNATQNKEQVNKQLVQNILNTAYSHL
                     KTCEQEYKAVSEKFNREKPFIKLQRRLEPCMDMLNQLYKEYGLKPPRIAVSTKAPDSL
                     DEYYKFALEKEKNMLETYRGFLKEEMPQDVRSAVHKLMELSANNAKVYRFILEAKNKL
                     NKARS"
     gene            3280..3783
                     /locus_tag="PLI11_05335"
     CDS             3280..3783
                     /locus_tag="PLI11_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPZ52321.1"
                     /translation="MFLDLARKRYSLRSFLNKEIEREKLEYVVEAARYAPSACNLQPV
                     KFYVVTDKDMLSKIHQAYPREWFKKAPCVIVAVGDRQSSWKRADGKDNVDVDVAISVD
                     HLTLAASDVGLGTCWVCAFDAKLCHEILNLPVHMEAIALIPIGYPEKEEVKEKRRKSL
                     DEIVEWI"
     gene            complement(3780..4025)
                     /locus_tag="PLI11_05340"
     CDS             complement(3780..4025)
                     /locus_tag="PLI11_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52322.1"
                     /translation="MKVKKVFVEVIAKFDPDGVVTPLAIIWEDGRRFEIDRVLDMRRA
                     ASLKAGGIGIRYTCRIRNKETYLYYEGPNWFVEQVIS"
     gene            complement(4015..>4274)
                     /locus_tag="PLI11_05345"
     CDS             complement(4015..>4274)
                     /locus_tag="PLI11_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52323.1"
                     /translation="HNGDPIRSIGVRACNLFVQSHVQLSFYPDVAQNERRYQLECAID
                     KIRNRFGRNILTRGVMLTQRKLSSFDPTSNTFGGKVSTHEG"
BASE COUNT         1212 a          816 c          899 g         1347 t
ORIGIN      
        1 tctacatatc aaaatcaatg aatgtaaagc ctgttaatat acttttagga acatccaagc
       61 ttgtgttaac agaccacact tcggtaaaaa ctgacaaatc atgcctgccg gacatatatg
      121 ctacattttg agagtttttt gaccagacca gtggagttgc atatgagttt gatatggcaa
      181 cttttctggc aagatttcca acaggctcag ttatcctgat tatcgagtaa tatcttccat
      241 tttcaaatac tgtttcgcta taggcaatcc tggaagaatc atgagaccaa acgggattga
      301 agtttttagc ctgctgccca cttggaagtt tgtttaaact tccgtcgttt agattaactg
      361 tgtatatttc agatatgctt gcaccgggtg atgtgaaaag aaaccagctg ccatcgggag
      421 atgcaagtac gtcattaatg ggatttgaaa attgaaacac tctttcttcc gtattgtcta
      481 ttaaattcaa cttgtagatg gacatgccgg attcgaatag aagtgaggct tcgtccagaa
      541 actgcacata tcttgccagc ggtctttcaa tggtttcaaa ctgccttgtc aacacattat
      601 acaaccttat taccgagctg tttgaataaa caattcggtt cccatgtttt gaccaggata
      661 caaaaccagg tacatcaatc ttatcaatgg aggaaacttc ttccgtgtcc ttgtctatga
      721 catatattat tccttcattg gatacataag agatgtgttt tccgttcgga gaaacaaaaa
      781 tacgtgcacc gtctcctcca tccggaacct taatgctttc aaagccgcct gtaaatccgt
      841 tatatataat aaggaagtga tttatatcat cgaagattat aagaacaaga ttattgaagg
      901 attcatccgg cgaaaaaggt attttcaggt ttgtgcctgg atggattatt ggtgtttcca
      961 gattgtttgc ttcaaatatt aaactgctgg atgtgttgta ccttctcgca atggaaaaca
     1021 atgtgtctcc tttcctgaca gtgtaccagg gcactccggc tggtactgtt aatacctggt
     1081 ctggataaat tgtgtacgga ggcgatatgt tgtttgcgtt gattagtgta tccaaggaaa
     1141 ttccatagaa acttgatatt ttaaaaagcg tgtctcccgg ttttactaca tatgaagtta
     1201 tacccatggg catggacaat tggcttccaa tctgtaacag gtttggctcc ctgatatgat
     1261 tcatatagac aagcgattca accggaactt gccatctgga agcaatattg tacaaagtgt
     1321 cacctttcct gacagtataa aaaatctgca ttaaatttcc ctctctttta atatcctgtt
     1381 ataatacaag tgtatgcgtg gaaacattat catataatct cagggagggg ttactttgaa
     1441 atatattaat gacatatgga ccagattgac agaaagcaag gttcttgttg ccgtgttggt
     1501 acaaggactc aaggttgtca gcatacttgc tgtttgtatt attgcctatc tcataacaac
     1561 aaaagttgta ctgaagctgc tgagcatgta tattaacaag acaaagtcat cctgggacga
     1621 tgcgtttttg aaaagaaaag tttttaccaa gttggcaaga ctagtgcctg cgataattat
     1681 atatgtatct gcgccaataa ttcctgaaat cagggttgtg attgaaaggg cggctttatc
     1741 tttcgtatac ttgaatgtag cctttgtttt atctgccgtt cttgatgctg caaacgacat
     1801 ttacatgaca tttccagtat ccaaggcaag accaattaaa ggatttttgc aaatcatcaa
     1861 gattgttgtt tatatcataa tgggcgtcct tgtgatttcc gtaatacttg acaagagtcc
     1921 ctggatacta cttagcagta tcggtgcttt gacagcgatt accactcttg ttttcaagga
     1981 ctccatcatg ggttttgttg caggtgttca gctcactaca aatgacatgc tcagaattgg
     2041 cgattggata gagatgccta aatatggagc ggatggtgat gttattgaaa taactttgaa
     2101 tacggtcaag gttagaaact gggacaagac cataaccacc atacctgcat acgctcttgt
     2161 ttctgattcc ttcaagaact ggaggggcat gcatgaaaca ggtggaagac gtatcaagag
     2221 ggcaatatat attgacgtga ccagtataaa gttctgtact cccgaaatgc ttgaaaggtt
     2281 taaaaagatt cagctgttga cagaatatat tgaaaacaag gaaaaagaga ttgaggagta
     2341 taacgaggag catgctatag ataccagcct gctggtaaat ggaaggcatt taaccaacat
     2401 aggtactttc agaaagtata ttgagtatta tttggagaac catcccagag ttcacaaggg
     2461 acttataagg atggtcaggc agctttctcc cgatgacagg ggactgcctc ttgagattta
     2521 tgcctttgtc gacaataccc aatgggttac ttacgagaat gtgcaggctg atatctttga
     2581 ccatatattt tcagtggttg atacttttga acttcgtata ttccagaagc cgtcaggaaa
     2641 cgacatgcag cttcttggat caagtctgaa ggattcctag cttctagctt tgtttaactt
     2701 gttttttgcc tcaaggatga acctgtatac ttttgcattg ttcgcagaca attccatcag
     2761 tttgtgaact gcactcctta catcctgggg catttcttct tttagaaaac ctctgtaggt
     2821 ttccagcatg ttcttttctt tttcaagtgc aaatttataa tattcgtcaa ggctgtccgg
     2881 agcctttgta ctcacagcta ttctgggtgg ttttagaccg tattccttgt ataactgatt
     2941 aagcatgtcc atgcaaggct ccagtcgtct ttgtaatttg ataaagggtt tttccctgtt
     3001 gaacttttct gaaactgcct tgtactcctg ctcgcaagtc ttaagatgcg agtaagctgt
     3061 attgagtatg ttttgaacaa gctgtttgtt tacctgctct ttattttgcg ttgcattaac
     3121 aaacaaactt gataatgtcg agattatcaa tataaggttt attattcttt tcatggtttc
     3181 atctcctccc attttatttt aaacccgtac tctgaaaata tacctttttg acaaaggtag
     3241 tgtctggcca tataatgtaa aaaagaaggg ggttttaata tgtttctcga tttggcaagg
     3301 aaaagatatt ctttgagaag ttttttaaac aaagagattg aaagggaaaa acttgaatac
     3361 gttgttgaag ctgcaagata tgcgccttct gcctgcaatc tgcagccggt taagttctat
     3421 gttgttactg acaaggacat gctttcaaaa atccaccagg cttatcccag ggaatggttc
     3481 aagaaagctc cttgtgtgat tgtggcggtg ggagacagac aaagctcctg gaaaagggcg
     3541 gatggaaaag acaatgtgga tgtcgatgta gcaattagcg tggaccacct gactctggca
     3601 gccagcgatg tgggccttgg cacatgctgg gtttgtgcct ttgatgcaaa attatgtcat
     3661 gaaatattga atttgcctgt ccacatggaa gcaatagccc tcatacccat aggatatccc
     3721 gaaaaggaag aagttaaaga aaaacgaagg aaatctctag atgagattgt ggagtggatt
     3781 taggaaatga cctgctccac aaaccagttt ggtccttcgt agtaaaggta tgtttccttg
     3841 tttctaattc tacaagtata tcgtattcct atgccgccag ctttcagact ggcggctctt
     3901 ctcatatcaa ggaccctgtc gatttcaaac cttcttccat cctcccaaat gatagccagg
     3961 ggtgtaacaa ctccatcagg atcgaatttt gcaataactt cgacaaaaac ctttttaacc
     4021 ttcatgtgtc gacacctttc ctccaaaagt atttgaagtc ggatcaaaag aagacagctt
     4081 tctttgtgtg agcattacgc ctcttgtcag tatgtttctt ccgaagcggt tgcgtatttt
     4141 gtcaattgca cattcaagtt ggtagcgtct ttcgttttgt gccacgtccg gatagaagga
     4201 aagctgcaca tggctttgca caaacagatt gcatgccctc acgccaatgg atctgatggg
     4261 gtctccgttg tgat
//
LOCUS       DAONVY010000158         4251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14733_length_4251_cov_3.419209, whole genome shotgun sequence.
ACCESSION   DAONVY010000158 DAONVY010000000
VERSION     DAONVY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14733_length_4251_cov_3.419209"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLI11_05350"
     CDS             <1..521
                     /locus_tag="PLI11_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52324.1"
                     /translation="LNLSTRKVERDYTDFTCINNYRLPKPRNFVTEIRVSDDEESIYF
                     FIKTDEDMVLKRHEKSNLVNLFLGFDIKDKNSFYSYKYVVNRYPGDSTTSIEKYENGF
                     VKVGDAEYRVDGNIMQIKMPKALFGIKDEFDMHFKVADSVENEDDILDYYVSGDSVPM
                     GRLSFLYSRIKQ"
     gene            538..2493
                     /locus_tag="PLI11_05355"
     CDS             538..2493
                     /locus_tag="PLI11_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52325.1"
                     /translation="MMKRLLSLTFAVFFLCIGLFSQVSSVGNSNNANKRSVTYITGTD
                     ALGRKIKQVADFRQDRYVGIFYFAWHNSDQHKVFDVTKILRENPDDLWSMDPKNPIAP
                     RATYYFNEPLYGYYSSSDPYVIRKHIELFIAAGIDFIAYDFTNFQIYWDPLYRMLDLL
                     IEYDNAGWKVPQVMFFTKKDCANQIPQLYEKLYMEEKYSHLWFRGSGSKPYLIGEPKD
                     IPEEYHDYFNLRYSFWPSDPYREDGWPYVDKVRPQRVFTNLVSVSVAQHTGGAFSWSI
                     KGPNNQQRPSWGRGYTSKNPVNGNIEAIERGDNFQEQWDTAIELDPEIIFVTGWNEWI
                     AGKFIDEDPKYGGVPYWVDTFNTEFSRDAEMTKSRGYVVDEKGNFLEEGYGDNFYMQM
                     VENIRRYKGLPITESNYVEPLSKTIDIFGDVSQWNDVNSVYLSHATEKVERDFRGYVA
                     RMKYTQAKPDNVVKEIRVTHDNDNLYFYIKTDNDITAYEKGKTNWMNLFIGIDGRKGN
                     SWEHYHYVVNRHPQEKSTSLEKFTNGYEGEVVSEVKYSVKGNVMQLEIPRKNLGLDDD
                     FSIYFKVADSIEKEDDIMDYYVSGESVPLGRLSFSYFGSLEAVKNLDEEPASSPQILK
                     WIILIACTIIICACILILGLGTKKQKL"
     gene            2506..3354
                     /locus_tag="PLI11_05360"
     CDS             2506..3354
                     /locus_tag="PLI11_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52326.1"
                     /translation="MKKIFLVLLATLIFAMSFSVHGEEERVNLINEHTETLEMIDAEF
                     DDGVFRITNPNKPVLALFGIPGLTNNYTWSGDVKIIEYGDDRTPNGVRLCVGYDVDTG
                     AYLNLIITRSIGIAAEVRGGGTYIPDVYKLTKEHFDRILDPGAEFHFEVIKNGTHVIL
                     KIDGDVVMDYVFPEEYDYFTEDDDYQIGFHAEKCAIEVRNLAVYCEDVEITPEPTETP
                     VPEETETPTPTEAPKTTPTPKKGKPATESDKGNEVDPFIIAVIVATLVIISVTVAVVV
                     IRRKKN"
     gene            3476..>4251
                     /locus_tag="PLI11_05365"
     CDS             3476..>4251
                     /locus_tag="PLI11_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52327.1"
                     /translation="MRNSLIKILVISLILCLVIPTLPTGAQEKKNLVTDSTTLIGSEN
                     GTFEDGVFKHTNPSGEHMTFLNIPNLINQYSWSGDVKILEYGTDSPANGVRFCIGHDK
                     DKNAYLNLILTRSVGISAEQRGAVYESDVYPLSKETFDRTLEPGDEFHFEIRKQGNYV
                     KMLVDGEVVMDTAFPSRYDFFTPGNEANIGFYSANCSFEVRNIEVYNEPDLLFNNQYT
                     LTFNDLALGELARGRYENWFVEWPSSNISSVRESGDLKYL"
BASE COUNT         1384 a          805 c          962 g         1100 t
ORIGIN      
        1 accttaacct gtccaccaga aaagtggaaa gggattatac cgactttaca tgcataaaca
       61 attacaggtt gccaaagcca agaaactttg taactgagat aagggtttcg gatgacgagg
      121 aaagcatata cttcttcatt aaaacagatg aagacatggt cttgaaaagg catgaaaaat
      181 cgaaccttgt aaacctgttc cttggttttg atattaaaga caagaattca ttttacagct
      241 acaagtatgt tgtaaacagg tatccgggtg acagcacgac ctcgatagag aaatatgaaa
      301 acggttttgt gaaggttggt gatgctgaat acagggtgga tggaaacata atgcagataa
      361 agatgcccaa ggctctgttt ggtattaaag acgagttcga tatgcacttc aaagtagctg
      421 acagtgtcga aaacgaagac gacattctcg actactatgt gtcaggagac tcggtgccaa
      481 tgggcaggct gtcatttttg tactcaagaa taaagcaata acggaaagag gtatgatatg
      541 atgaaaagac tattatcatt gacatttgcg gttttctttc tgtgtatcgg cttgttttcc
      601 caggtttcca gtgtgggaaa ctcaaacaat gccaacaaaa ggtcggttac atatattacc
      661 ggaaccgatg ctttgggaag gaaaatcaaa caggtggcgg atttcagaca ggacaggtat
      721 gtcggaatct tctactttgc ctggcataac tcagaccagc ataaagtgtt tgacgtaacg
      781 aaaattctta gggaaaaccc ggatgatttg tggagcatgg atcctaaaaa ccccatagct
      841 ccaagagcaa cctactactt caacgagcct ttgtatggct actacagctc atccgaccct
      901 tacgtaatca gaaagcatat tgagcttttc atagcagctg gcattgattt tatcgcctat
      961 gactttacaa acttccaaat ttactgggat ccgttgtaca gaatgctgga tctgctaatt
     1021 gaatacgaca atgccggctg gaaggttcca caggttatgt tctttacaaa gaaagactgc
     1081 gcaaatcaga tcccacagtt gtatgaaaaa ctgtatatgg aagagaaata ctcccatctt
     1141 tggtttagag gcagtggcag caaaccctat ctgataggag aaccaaaaga catacctgag
     1201 gaatatcacg actacttcaa tctaaggtac tccttctggc cttccgatcc atacagagaa
     1261 gacggctggc cttatgtgga caaggtacgc ccacaaaggg tgtttacaaa ccttgtgagc
     1321 gtatcggtag cccagcatac gggaggcgcc ttcagctggt caataaaagg accaaacaac
     1381 cagcaaaggc cgagctgggg aagaggatac acctcaaaga atcctgtcaa tggaaacatt
     1441 gaagcaatag aaagaggaga caacttccag gagcagtggg atacagctat agagttggat
     1501 ccggaaatca ttttcgttac tggttggaac gaatggattg caggcaagtt catcgacgag
     1561 gatccgaaat acggcggcgt cccctactgg gtggatacct tcaataccga attctcaaga
     1621 gatgcggaga tgaccaaatc aagagggtat gtggtggatg agaaaggcaa cttccttgaa
     1681 gagggttacg gcgacaactt ctacatgcag atggttgaaa atattagaag gtacaaaggc
     1741 ttacccatta ccgaatcaaa ttatgtggaa cctttgtcaa aaacaataga catctttggc
     1801 gatgtttctc aatggaatga tgtcaatagt gtatacctaa gccacgctac tgagaaggtt
     1861 gaaagggatt ttagaggata tgtggcaaga atgaagtaca ctcaggcaaa gcctgataac
     1921 gttgtaaagg aaatccgtgt aactcacgat aatgacaatt tgtatttcta cataaagacg
     1981 gataacgaca ttactgctta cgagaaagga aaaaccaact ggatgaatct ttttattgga
     2041 attgatggca ggaagggcaa ttcctgggag cactaccact acgttgtaaa caggcaccct
     2101 caagagaaga gcacttcctt ggaaaagttc acaaacggtt acgaaggcga ggttgtttcc
     2161 gaagttaaat acagtgttaa aggtaatgtt atgcagctgg agataccaag gaaaaacctg
     2221 ggccttgatg acgacttctc catctatttc aaggttgcag attccatcga gaaagaagac
     2281 gacattatgg actattacgt ttccggagaa tccgtgcctt taggaagact ctctttcagt
     2341 tatttcggca gtctggaagc tgtgaaaaat ttggatgagg aacccgcaag ttctccccaa
     2401 atacttaagt ggattattct tattgcttgc acgattatta tttgcgcttg tattttaatc
     2461 cttggattgg gaacaaagaa acaaaaattg taaggagatg atggtatgaa aaagattttt
     2521 cttgttttac tagctacatt aatatttgcc atgtcttttt ctgtacatgg tgaagaagaa
     2581 agagtaaatc tcataaacga gcatacggag acccttgaaa tgattgatgc agagtttgac
     2641 gacggggtat ttagaataac gaaccccaac aaaccagttc tggcactttt tggaatccca
     2701 ggtctgacaa acaactatac ttggtcagga gacgtcaaaa tcattgagta tggtgatgat
     2761 agaactccaa atggagtgag actctgcgta ggatacgatg ttgatacagg agcgtatctt
     2821 aaccttatca tcacccgaag cataggcatt gcagctgaag ttcgcggagg aggaacttat
     2881 attcccgatg tatataagct tacaaaggaa cattttgaca ggatacttga cccaggcgcc
     2941 gagttccact ttgaagttat taaaaatggt actcatgtta tcctgaagat tgacggagat
     3001 gtggtaatgg attatgtctt cccagaagag tatgactact tcacagaaga cgatgactac
     3061 cagattggat ttcatgcgga aaagtgtgcc attgaggtta gaaacctggc agtttactgc
     3121 gaggatgtgg agattacacc agaacctacg gaaactccag ttcctgagga aactgaaact
     3181 ccaaccccaa ccgaagcacc aaaaacgaca ccaactccaa agaaaggaaa accagctacg
     3241 gaatcagata aaggaaatga agttgatcca tttataattg cagtgattgt cgcaacactt
     3301 gttataattt ccgttactgt ggccgtggtg gttattagaa ggaaaaagaa ctaaatgagc
     3361 actagatgtt aaaaatagta taacttttgg ttaatttata tactactttt tacaacctga
     3421 tttatctata atatttatag agacaagttg tagattaata aatgggagga ttaaaatgag
     3481 aaactcttta attaaaatct tggtaatcag tttaattctg tgccttgtta ttcccacact
     3541 gccaacaggc gcacaagaaa aaaagaatct ggtaactgac agcactactc tcataggtag
     3601 tgaaaatgga acctttgagg atggcgtgtt caagcacacc aacccaagcg gcgagcacat
     3661 gacatttctc aatatcccaa atctgattaa ccagtattct tggtcaggag atgttaaaat
     3721 cctagaatac ggcaccgact ccccagcaaa cggagtaagg ttctgtatcg gacatgacaa
     3781 agacaagaat gcctatctca acctgatact tacccgcagc gtaggtattt ctgctgagca
     3841 aagaggagcg gtttatgaga gcgacgttta tcctctttcc aaggaaacct tcgatagaac
     3901 gctggaaccg ggtgatgaat tccattttga aatcagaaaa cagggtaatt atgtaaagat
     3961 gcttgttgac ggagaagttg tcatggatac agcattccca agcagatatg acttctttac
     4021 ccctggcaac gaggcaaaca taggcttcta tagtgcaaac tgttcttttg aagttagaaa
     4081 cattgaagta tataacgaac cagatcttct gttcaacaat cagtacacct tgactttcaa
     4141 tgatctggca ctgggcgaac tggcaagagg cagatacgaa aactggtttg tcgaatggcc
     4201 atcaagcaat atttcctcgg tgagagaaag cggagatctg aaatatcttg a
//
LOCUS       DAONVY010000159         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14921_length_4206_cov_3.817393, whole genome shotgun sequence.
ACCESSION   DAONVY010000159 DAONVY010000000
VERSION     DAONVY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14921_length_4206_cov_3.817393"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="PLI11_05370"
     CDS             <1..1602
                     /locus_tag="PLI11_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52328.1"
                     /translation="YFGYYRNTDAWVYRKHAYMLSAAGIDFIFIDISNEPTFNEAHTL
                     LFDTWLQMRKEGFKTPQIMFLTGDAEGRLGSHLRQLRRTVYSDENWDKYEELFFKWEG
                     KPLIFGNPKGLTEDMQKLINEKFTLRGSWAWKDEDGYWNWIMEYPQAKGRSFEGVFEQ
                     MAVTMGHHPSASKGRSFVSGKQPNNGKEDFEFSSDTARYGLSFKQQFEYALEMDPQVI
                     MITGWNEWIAGKPTGDELNYFANTPVNGYTYVDQFNPEFSRDGEPMKIRDGVGFGDNF
                     YYQMVGYIRKFKGLNEIEKAKNQKTININGGMSQWDDIGPEFRDTIGDTKFRNEPSYD
                     LDFRYINNTGRNDFDYAKVSQDNENIYFMVKTVNDIVHADGPNWMNLFIDLDQSHKTG
                     WEGYDYIINRTGNNGKCTIERFKNNSWDFEKVGEARYTVNGQYMMVSVPKKALGISDK
                     AVSFDFKWADNSTTSGDVMQFMDLGDAAPNDRFKFRYIASTSIFDNLINTILIIGGAV
                     VLLVVVSVIVFVLLRRRKKRSMQFM"
     gene            complement(1796..2233)
                     /locus_tag="PLI11_05375"
     CDS             complement(1796..2233)
                     /locus_tag="PLI11_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPZ52329.1"
                     /translation="MICILIAILSGALMSIQGVFNTELTNDTSIWVSVSFVQLSALIT
                     SLCVWFISDRNNSFKKLFHVQPRYILLGGIIGAFITYTVIKAVDKLGPAKAVMIIVIS
                     QLLTAYLIELFGMFGTEKVSFSIRKIIGLILAIIGIVIFKWNC"
     gene            2396..3331
                     /gene="fba"
                     /locus_tag="PLI11_05380"
     CDS             2396..3331
                     /gene="fba"
                     /locus_tag="PLI11_05380"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPZ52330.1"
                     /translation="MPLVTTKEMFENAYKGGYAIGAFNVNNMEIIQGITEAAKEVNAP
                     LILQVSAGARKYANHTYLMKLVEAAVIETGLPIALHLDHGDSFELCKSCIDGGFTSVM
                     IDGSHLPFEENIALTKKVVEYAHERGVVVEGELGRLAGIEDAVNVSEEDAMYTNPDEV
                     QEFVEKTGVDSLAIAIGTSHGAYKFKPGQKPQLRFDILEEIQNRLPGFPIVLHGASSV
                     VPEFVEMINKYGGNMPDAIGIPEDMLRKAASMAVCKINIDSDLRLAMTASIRKYMTEN
                     PSHFDPRQYLKPARAAIKELVKHKIVNVLGCDGKA"
     gene            3359..>4206
                     /locus_tag="PLI11_05385"
     CDS             3359..>4206
                     /locus_tag="PLI11_05385"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPZ52331.1"
                     /translation="MSVLKGAAIFGQSGGPTSVINASAAGVFLTALQQDCITKVYGAA
                     HGIKGILEEQFYDIGMEDKYELELLKTTPSSALGSVRYKLADPDVDDTDFKRLLEVFK
                     KYNIRYFFYNGGNDSMDTCNKVSKFMKQAGYECRCMGVPKTIDNDLFGTDHSPGYGSA
                     AKYVATSTMEVFHDARVYDTGMVAILEVMGRNAGWLTAATALAAYKGQGPDLIYVPEL
                     PFDDEKFLSDVKRVYENNNKVIVAVSEGLRYADGTYVADSGVRDSFGHAQLGGVAYKL
                     ADMAKSA"
BASE COUNT         1351 a          721 c          968 g         1166 t
ORIGIN      
        1 tatttcggct attacagaaa cacggatgca tgggtgtata gaaaacatgc atacatgctg
       61 tctgctgcag gaattgactt tatcttcatt gacatctcca acgagcccac ttttaatgaa
      121 gctcatacac tgttgtttga tacctggctt cagatgagga aggaaggatt caagactcca
      181 caaatcatgt ttttgactgg agatgcggaa ggaagacttg gttcccatct gagacagctt
      241 cgcagaactg tctacagtga cgagaattgg gataaatatg aagagctttt cttcaaatgg
      301 gaaggcaagc ctctcatttt tggaaatccc aaaggtttga ctgaagacat gcagaagttg
      361 atcaatgaaa agtttacgct gagaggcagc tgggcatgga aagatgagga tggatactgg
      421 aactggatta tggagtatcc gcaggcgaaa ggaagaagct ttgaaggagt atttgagcag
      481 atggcagtta caatgggtca tcatccttca gccagcaagg gaagaagctt tgtaagcggc
      541 aaacagccaa acaacggcaa ggaggacttt gagttttcat cagataccgc acggtatggt
      601 ttgtccttta agcaacagtt tgagtatgca ttggaaatgg atccccaggt aatcatgata
      661 accgggtgga atgaatggat tgcgggcaag ccaacaggag atgaattaaa ctattttgca
      721 aacactcctg ttaatggcta tacatatgtg gatcagttca atccagagtt ctcaagggac
      781 ggagaaccta tgaagatacg tgatggtgtt ggctttggag acaacttcta ctatcaaatg
      841 gtaggatata taaggaagtt caagggactg aacgaaatag aaaaagctaa aaatcagaaa
      901 acaataaata ttaatggggg catgtcccag tgggatgata ttggtcctga gttcagggat
      961 acaatagggg atacgaagtt cagaaacgag ccttcgtacg atcttgattt caggtatatt
     1021 aacaatactg gaagaaacga ctttgattac gctaaagtat cccaggacaa tgaaaacata
     1081 tacttcatgg taaagacagt gaacgacatt gttcatgctg acggtcctaa ctggatgaac
     1141 ctgtttattg accttgatca aagccataaa actggctggg aaggttatga ttacataatc
     1201 aacaggaccg gaaataacgg caaatgcaca attgaaagat tcaaaaacaa tagctgggat
     1261 tttgaaaagg ttggggaagc aagatatact gtaaatggcc aatatatgat ggtaagtgtt
     1321 ccaaagaaag cattgggtat aagcgacaag gccgtttcct ttgatttcaa atgggctgac
     1381 aactccacaa catctggaga tgtaatgcaa tttatggatc ttggagatgc tgctccaaat
     1441 gatcgcttca agtttagata tattgcttca acctccatct ttgacaacct aataaacacc
     1501 attttaatta ttggtggtgc agtggtatta ctggttgttg ttagtgttat agtttttgtc
     1561 ttgcttagaa gaagaaagaa aagaagcatg cagtttatgt aaaggggctg tcgcattagt
     1621 cggttttcag actgatgcga cgcttttttt tgttattagc tgtaagtata ttctgaattg
     1681 caggtggtca aagaagtgtt agaaaggttt ttctccttat agtacttgcc aaaaccttta
     1741 tttaaatcaa tggcgtttgc cttctagatg atggaatgat ggggtattat tgcttttaac
     1801 aattccattt aaatattacg ataccgatta ttgcgagtat cagcccaatt attttcctga
     1861 tagagaagga tactttttct gttccgaaca ttccaaacaa ttctattaaa tacgctgtta
     1921 aaagttgtga tatcacaatt atcataacag cctttgcagg tccaagcttg tctactgcct
     1981 ttatgacagt ataagttatg aatgcaccta taattccgcc aagaagaatg tatcttggct
     2041 gcacatggaa aagcttttta aagctgttat ttctatctga gatgaaccag acacataggc
     2101 tggttataag cgctgaaagc tgtacaaagc ttactgaaac ccatatgctg gtgtcatttg
     2161 taagttcagt attgaaaact ccttgtatac tcattaatgc gcctgacaaa atggcaataa
     2221 gaatacaaat catagcgtca cccaatttta ttgatatact ctattttgct gcatacatca
     2281 gatagatatg cagctaatat tttcataatt atcaactaaa cttgataatt attctacaaa
     2341 gtgataaaat acctaaatgt agagaatttt attaattaaa aaggagagag gataaatgcc
     2401 attagtaaca acgaaagaaa tgtttgaaaa tgcctataaa ggcggatacg caataggtgc
     2461 tttcaacgta aacaacatgg agattataca aggaattacc gaggctgcaa aggaagttaa
     2521 tgctccctta atccttcagg tatctgctgg tgcaagaaag tatgcaaacc acacatatct
     2581 tatgaaattg gtagaagcag ctgtaataga gactggcttg ccaatcgctt tgcaccttga
     2641 ccatggtgac agctttgaat tgtgcaaatc ctgtatagat ggcggcttta catctgttat
     2701 gatagacggt tcacatctgc cctttgagga aaacatagct cttacaaaga aagttgtaga
     2761 gtatgcccat gaaaggggag ttgttgtaga aggcgaactg ggaagactgg ctggtataga
     2821 agacgctgtt aatgtaagtg aagaggatgc aatgtacaca aatcctgatg aagttcagga
     2881 gtttgttgag aaaacaggtg ttgactccct cgcaattgca attggaacaa gccatggcgc
     2941 atacaaattc aaacctggtc agaagccaca gctaagattt gacattcttg aagaaattca
     3001 aaacagactg cccggtttcc caatcgttct gcatggcgca tcttctgttg tacctgagtt
     3061 tgtagaaatg attaacaagt atggtggaaa catgcctgat gctatcggta ttccagaaga
     3121 tatgcttcgc aaggcggctt ccatggcagt ttgcaagatt aacattgact ctgacttgag
     3181 acttgccatg acagcatcca ttagaaagta tatgactgag aatccatcac acttcgaccc
     3241 aagacagtat ttgaaaccag caagagctgc aataaaggaa cttgtaaagc ataagatagt
     3301 aaacgtattg ggttgcgacg gaaaagctta agtatagttt atgaaggaga gaagttaaat
     3361 gagtgtattg aaaggcgctg caatattcgg tcaatcaggc ggaccaacat cagttattaa
     3421 tgcaagtgct gcaggtgtat ttttgactgc acttcagcaa gattgcatta ctaaggttta
     3481 tggagccgcc cacggtataa aaggcatttt ggaggaacaa ttttatgata taggaatgga
     3541 agacaagtat gagttggaac tcttgaagac aactccatct tccgctcttg gctcagtaag
     3601 atacaaactg gctgatccag atgttgatga tacggatttc aagcgacttc ttgaagtatt
     3661 taagaaatat aacattaggt acttcttcta taacggtgga aatgactcca tggatacctg
     3721 caacaaggtt agcaagttca tgaagcaggc aggatacgag tgcagatgca tgggagttcc
     3781 caagaccata gataacgacc tgtttggtac agaccacagc cccggctatg gaagtgcagc
     3841 caagtatgtt gctacttcta ccatggaagt attccatgat gctagagtct atgatacagg
     3901 catggttgca attcttgaag tcatgggaag aaatgcaggt tggttgacag cagctactgc
     3961 acttgcagca tacaagggac aaggacctga tttgatctat gtgcctgaac ttccatttga
     4021 tgatgaaaaa ttcctttctg atgttaagag agtatatgag aataataaca aggtcatagt
     4081 tgcagtatca gaaggattaa gatatgcaga tggaacctat gtggcagaca gcggtgtaag
     4141 ggacagcttt ggacatgcac agctgggtgg agttgcttac aagctggcgg atatggcaaa
     4201 gagtgc
//
LOCUS       DAONVY010000160         4197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14952_length_4197_cov_2.764606, whole genome shotgun sequence.
ACCESSION   DAONVY010000160 DAONVY010000000
VERSION     DAONVY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14952_length_4197_cov_2.764606"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1822
                     /gene="polA"
                     /locus_tag="PLI11_05390"
     CDS             <1..1822
                     /gene="polA"
                     /locus_tag="PLI11_05390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPZ52332.1"
                     /translation="VLNKLEFRSIIKRLNLEQVEPEAEVVFEKENIYKDIEDMILPEK
                     VVELSGIDDMTNLILPEKIDVAYITYSLDNSGLSEVNFRFDDSNVGYTVILPGALFVP
                     NFMDKFASVFENESIKKVLFDAKPFILWLKNNGCDFKGLYCDVAVCAYLSDSTRKSNG
                     ITDVYRFYTSKEIKQGSLNELFALKEIQEYAFRRLEEDGQTQLFEDVELPLVCILADL
                     EYTGFRVNPDILKAEGRDFDKRINELTGLIHDYAGKAFNINSPKQLGEVLFEDLKLEV
                     KKKTKTGYSTNIEVLEKLYDAHPIIPCIIEYRQNTKLKSTYIDGLLSAINLMTGRVHS
                     SFNQMATATGRLSSTEPNLQNLPIKSEIGRHIRRAFEPRDENHILIDADYSQIELRVL
                     AHISQDSTMIEAFRNNEDIHTVTAAKVFNVGLEDVTYEMRSKAKAVNFGIIYGISEYG
                     LSRDLNISFYEAKQLKTDYLKKYPGVAAYMKDIVESARKLGYVTTLMGRRRYLPELAS
                     SNNQIRSFGERIALNTPIQGTAADIIKIAMVKVYNELARRKLESKLILQVHDELLIDA
                     LKEEKEEVIALLKSCMENAFELSVPLVVSIDTGMNFDFNE"
     gene            1836..2438
                     /gene="coaE"
                     /locus_tag="PLI11_05395"
     CDS             1836..2438
                     /gene="coaE"
                     /locus_tag="PLI11_05395"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520919.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPZ52333.1"
                     /translation="MRILGVTGGTGSGKSLVCQILKKQGAEIIDADEITRELQKKGSD
                     VYNEMVRFFGDDIVKADGELDRKKIAELVFKDKEMLLKLNHIVHKHVAAEIKKRVAAL
                     KEQGSGLVVLDVPIPVEEGFFDTAECIWTVVANADLRVERIMKRMNISEEQALTRINA
                     QLSNKEYEEIADVIIENEGSYEELEDLVLFELKRFLARLC"
     gene            2451..3779
                     /locus_tag="PLI11_05400"
     CDS             2451..3779
                     /locus_tag="PLI11_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPZ52334.1"
                     /translation="MGFPKDFLWGVATSAAQIEGAAFDDGKGASIWDVFASKPGKTFN
                     GETPSRACNSYYMFDEDLKNLKELGVNSYRFSISWPRVLPKGTGKLNEKGMDYYKRVI
                     NGLLENDIIPNVTLYHWDLPQALEDKGGFANRDVIEWFGEYAHKMFVAFGDVVPMWST
                     VNEPIAVYVGYAHGGFAPGYTDEKKGNQARHNILVAHGNAVRAFRAENLKNGRIGIVI
                     DMWKRHALTDSEEDKALERDEDERNWKFYFDPVLKGSYSDYILTKLEEEGTLMDIKPG
                     DLEMANEPIDYFGLNVYNRVVVSKDQKAIMDFSQGGNFLNNRTEYYPKAVYDALHILK
                     DKYNLRIPVYITENGTYSVGEEKVNEKTGMIEDDDRIRYVSGFFSWLEKAIDEGFDVR
                     GYYLWTLMDNFEWSAGSNYRFGIIHTDFDTCERTWKKSAYWFRDYINSKR"
     gene            3798..>4197
                     /locus_tag="PLI11_05405"
     CDS             3798..>4197
                     /locus_tag="PLI11_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52335.1"
                     /translation="MSFTSRVLCLLLAILMLLPIGCTKEEEDLKIPKGWLRRIPLGNS
                     DVASKYLFGPNQCEFDFVKDKKEGNVLKLSTSVDSATDPYIHFNFRDYIKEIGEKDLF
                     ADDFDVVMLRVRVENLTGSNFEMYYANGVST"
BASE COUNT         1325 a          649 c         1087 g         1136 t
ORIGIN      
        1 agttcttaac aaacttgagt tcaggtcaat tattaaaaga ctcaatctgg aacaggttga
       61 accagaggct gaagttgttt ttgagaagga aaacatttat aaggacatag aagatatgat
      121 acttcctgag aaagttgttg agctttcggg tatcgatgat atgacaaatt taatactgcc
      181 tgaaaagatt gatgttgcat acatcacata cagcttggac aatagcggac tttccgaggt
      241 aaatttcagg tttgatgact ccaatgtggg ttatacggta atacttccag gagccttgtt
      301 tgttccaaat tttatggaca agtttgcttc tgtatttgaa aatgagagta taaagaaagt
      361 tttgtttgat gcaaaaccat ttattctgtg gcttaagaac aatggttgtg attttaaagg
      421 tttgtactgc gatgtggctg tttgtgccta tttgtccgat tcaaccagaa agtcaaacgg
      481 tataacggat gtatacaggt tttatacatc aaaagaaata aagcagggca gtcttaatga
      541 gctttttgca ctaaaggaaa tacaggaata tgcctttaga aggcttgagg aggatggaca
      601 gacacagctc ttcgaggatg tggaacttcc tcttgtatgt attcttgcgg atttggagta
      661 tacagggttc agggttaatc ctgacatatt gaaagctgaa ggcagggatt ttgacaaaag
      721 gataaacgaa cttacaggct tgatacatga ttatgccggg aaagctttta atattaattc
      781 gccaaagcaa ttaggcgagg ttctttttga ggatttgaag ctggaggtaa aaaagaaaac
      841 taaaacaggt tacagtacca atattgaggt tttggaaaag ctttatgatg cccaccccat
      901 aatcccctgc attattgaat acaggcagaa tacaaaactt aagtcgacat atattgacgg
      961 gcttttgtca gcaataaatc ttatgacggg aagggtgcat tcgagtttca atcagatggc
     1021 aacagcgaca ggaaggttaa gcagtacgga gcccaatttg caaaacctgc ctataaagag
     1081 cgagatagga aggcatataa ggagggcctt tgaaccaagg gacgaaaacc atatcctaat
     1141 agatgccgac tactcgcaga ttgaactgcg ggtccttgcc cacatttcac aggacagtac
     1201 gatgattgaa gccttcagga acaatgagga tattcataca gtaactgcag ccaaggtttt
     1261 caatgtaggt ttggaggatg ttacgtatga aatgcgctcc aaggcaaagg ctgtcaactt
     1321 cggtataata tatggtatta gtgagtatgg cttgtccagg gatctaaata tcagttttta
     1381 tgaggcaaaa cagcttaaga cggattatct taaaaaatat ccgggtgttg ccgcatacat
     1441 gaaggatatt gtggagagcg ccaggaaact tggatatgtc acgactttga tgggcagaag
     1501 aagatatttg ccggaacttg cttcctccaa caatcagatt agatcttttg gtgaaagaat
     1561 agctctaaac acgcccatac agggaacagc agccgatatt atcaagattg ccatggtaaa
     1621 ggtgtacaac gagcttgcaa ggaggaagct tgaatccaag ctcatactgc aggtgcatga
     1681 cgagttgttg attgatgccc ttaaagaaga gaaggaagag gtaatcgcct tgttaaagag
     1741 ttgcatggaa aatgcctttg agttatcagt tccgttggtg gtgagcattg atactggaat
     1801 gaattttgat tttaatgagt aggcaggtgg taagtgtgcg cattttaggt gtaacgggag
     1861 gaacaggtag tggaaaatct cttgtatgcc agatattaaa aaagcaggga gcagaaataa
     1921 ttgatgctga cgagataacc agagagcttc agaagaaggg aagcgatgtg tacaacgaga
     1981 tggttcgttt ctttggagat gacatagtga aagcagatgg agaactggat aggaagaaga
     2041 ttgcagagct ggttttcaaa gataaagaaa tgctccttaa actgaatcat atcgtccaca
     2101 agcatgtggc ggccgaaatt aagaaaaggg tggctgcact gaaggaacaa ggtagtggac
     2161 ttgttgtttt ggatgttcct atccctgtgg aagaaggttt tttcgatact gccgagtgca
     2221 tatggacagt ggtggctaat gcagatctta gggtggaaag aatcatgaag aggatgaata
     2281 tatccgaaga acaggcattg acaaggataa atgcacagtt atccaataaa gagtatgaag
     2341 aaattgcgga tgtcattatt gaaaatgaag gaagctacga ggaactggag gacctcgtat
     2401 tgtttgaatt gaaacgtttt cttgcaagat tgtgttagga gggtgttatc gtgggatttc
     2461 caaaggattt tttatggggt gttgcgacaa gtgctgcaca gattgaaggt gctgcttttg
     2521 acgatggcaa aggtgccagc atatgggatg tatttgcatc gaagccgggt aagaccttta
     2581 acggtgagac tccgtccaga gcgtgcaaca gctactacat gtttgacgag gatttgaaga
     2641 accttaagga gcttggcgta aattcataca ggttttccat atcatggcca agggttttgc
     2701 ctaaaggaac aggaaaactg aacgaaaagg gcatggacta ttacaaaaga gtaattaatg
     2761 ggttgctgga aaatgacata attccaaacg ttacattgta tcactgggat cttccccagg
     2821 ctcttgagga caagggcgga tttgcaaaca gggatgttat cgaatggttt ggagagtatg
     2881 cgcataaaat gtttgtggct tttggtgatg tggttcccat gtggtcaaca gtaaatgagc
     2941 ctattgcagt atatgtgggg tatgcgcatg gcggctttgc tcccggatat acggatgaga
     3001 aaaagggcaa tcaggcaaga cacaatattc ttgttgccca tggtaatgca gtaagagcgt
     3061 tcagggcaga gaatttgaaa aatggcagaa ttggtattgt gattgacatg tggaagagac
     3121 atgctcttac agatagtgag gaagacaagg ctctggaaag ggatgaggat gagaggaact
     3181 ggaagtttta ctttgatccg gttctaaaag gaagctacag cgattatatt ttaaccaagc
     3241 tggaggaaga gggcaccctt atggacatca aaccgggtga cttggaaatg gcaaacgagc
     3301 caattgacta tttcggccta aatgtataca acagggtggt tgtgtcaaaa gatcagaaag
     3361 caatcatgga cttttcacag ggtggaaact tccttaacaa cagaacggaa tattatccaa
     3421 aagccgtcta tgatgccttg catatattaa aggataaata caaccttcgg atcccagtat
     3481 atattacaga aaacggcact tacagcgtag gggaagagaa ggtaaatgaa aagaccggca
     3541 tgatagagga tgacgacagg attaggtatg taagcggatt ttttagctgg ttggaaaaag
     3601 ccatcgatga aggttttgat gtcagaggtt actatttatg gaccttgatg gacaactttg
     3661 agtggtcggc aggatcaaat tacagatttg gaattattca tacggatttt gatacttgtg
     3721 aaagaacatg gaagaagagt gcgtactggt tcagagatta tattaattca aaaagataaa
     3781 aaaggagaag tggttacatg agctttactt caagggtgtt atgtttgttg cttgctattc
     3841 tcatgcttct gcccattgga tgtacaaagg aggaagagga cttgaaaatt ccaaagggct
     3901 ggctgagaag aattccgttg ggcaacagtg acgttgcttc caagtatttg tttggcccga
     3961 atcagtgtga gtttgatttc gtcaaggaca aaaaggaagg aaatgtcctc aaattgtcca
     4021 catctgttga ttctgcaaca gacccatata ttcatttcaa tttccgtgac tatataaagg
     4081 aaattggcga aaaggatctg tttgcggatg attttgatgt ggttatgctc agagtacggg
     4141 tagagaacct gacgggaagc aattttgaga tgtactatgc caatggagtc agcaccg
//
LOCUS       DAONVY010000161         4196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_14956_length_4196_cov_3.310070, whole genome shotgun sequence.
ACCESSION   DAONVY010000161 DAONVY010000000
VERSION     DAONVY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14956_length_4196_cov_3.310070"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="PLI11_05410"
     CDS             complement(<1..1790)
                     /locus_tag="PLI11_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPZ52336.1"
                     /translation="MDGNIVIQSVNAVHGSKKFIVKAVADTFVSYNKIKEIKKKICKK
                     IGYDKVILQLSCKACMESVINGEDFWENMLSYFKEENNSVWALLEGSKCELRNKRLVV
                     DLDKKVAGILKEKQVDKFIADQIKMMWDEHVEIDFSDCDVEIEGINTEVVKVDFTSQD
                     HDRAIQESIKRNEEKLRQQKYNGSNGTSNGGNGNFVRRNGGGSRRGNKLNFQEDGSLP
                     KDSKGNEIIYGREIDMPITPMKDVDVYTGQVAIRGRVVGIEIKEIANEKAICSFHIAD
                     NTGAVTVKFFFEQKDLKAVNGAIKKNMHFTVMGKTEFDPFTHELSISAWSIVRFKPVE
                     KEDNAPVKRVELHLHTNMSPQDAVTRPEQLIPRALKWGHKAIAITDHGVVQAYPEVYK
                     AAKNKDIKIIYGVECYLTNQMEEMPLFKAKQFKSYHCILLVKNQVGLKNLYKLISRSH
                     IDHFYKRPRIPKYILDAHREGILVGSACCEGELFNAFLENKSTEEIEKIASYYDYFEI
                     QPVGNNMFLIREGILNSVKDIERINTEIVKLGDKHGKMTVATGDVHFLEENDSIYRAV
                     MMGAQGYSDADLQPPLFFRTTEEMLNEFTYL"
     gene            complement(1794..2870)
                     /gene="ispG"
                     /locus_tag="PLI11_05415"
                     /gene_synonym="gcpE"
     CDS             complement(1794..2870)
                     /gene="ispG"
                     /locus_tag="PLI11_05415"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPZ52337.1"
                     /translation="MQLYLRNETRVVRAGNVYIGGGNRISVQSMTNTDTRDVKSTVKQ
                     IRMLEEAGCDIVRVAVPDLEAAIAVKEIKKQINVPLVSDIHFDYRLALECIKNGVDKV
                     RINPGNIGDRDRVEQVVKAAKERNIPIRIGINSGSLDKSIKERYGLSKEALVESAMEH
                     IKILEGLDFYDIVISLKATNIKLCVDSYMLMANMRNYPLHVGVTEAGTFLSGTIKSSA
                     GIGAILMQGIGDTIRVSLTGDPVREIEVGKSLLAALELGGRNFTFISCPTCGRTELDL
                     TGIAEEVEKALFEMDGKGEIKRHITVAVMGCAVNGPGEASYADIGIAGGRNEALLFSN
                     GKIVGKLKGDSIAEQFIQKIRELG"
     gene            complement(2883..3941)
                     /locus_tag="PLI11_05420"
     CDS             complement(2883..3941)
                     /locus_tag="PLI11_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPZ52338.1"
                     /translation="MGMLGSVLIMLFMLSILVVVHEFGHFFAARLFKVKVEEFSIFMG
                     PKIFSRVSKKTGTRWSIRALPIGGFCLMEGEEETVQSSTSFDSKPWYARAVILLAGAL
                     MNLLLAILIIAIMFSFFGYETNKVSEVLVAEEGTYPAQELGFEVGDRIISYDGKRVHT
                     PNDYRIFEMVDKDMTSEIEIKKADGTVRKHTIEREKLENGAKSYIGFRFGVEKGNFFG
                     ILGNTMLYIFSLIKSVFYAIFWLITGQLGLDAVASPIGMTGIVNEVVQQDASWLPKLL
                     ALINMTALISANLAVFNLLIVPGLDGGKLLFILIELLRGGKKISPETEAKFSYVGIGL
                     LLLLALVVAGNDILRLIR"
     gene            complement(3925..>4196)
                     /locus_tag="PLI11_05425"
     CDS             complement(3925..>4196)
                     /locus_tag="PLI11_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPZ52339.1"
                     /translation="PDMDRFPCLKYAYEAARIGGTMPCVMNAANEVCVESFLRGEIPF
                     MMISEIVGKVMEGHKSLPSPDLEDILRCDLEARRTAGGIINGYVR"
BASE COUNT         1094 a         1072 c          696 g         1334 t
ORIGIN      
        1 agatatgtaa actcgttaag catttcctcc gtagtcctga aaaacaacgg aggctggagg
       61 tcggcatcag agtatccttg agcacccatc attaccgccc tgtatatgga atcattctcc
      121 tcaagaaaat gcacatctcc cgtggcaaca gtcattttcc catgtttgtc tccaagtttt
      181 acaatctctg tatttattct ctcaatatcc tttacactgt tcaaaatacc ctcgcggatt
      241 aagaacatgt tgtttccaac cggctgtatt tcaaaatagt cgtagtatga agcaattttc
      301 tcaatttcct cagtgctttt gttttccaaa aatgcattaa aaagttctcc ctcacagcag
      361 gcactgccta caagaattcc ttccctgtga gcatccagta tgtacttggg tattctcggt
      421 cttttgtaga aatgatctat atgggacctc gatatcaatt tatacaagtt tttaagtccc
      481 acctgattct taaccaaaag aatgcagtgg tatgacttga actgttttgc cttgaacaag
      541 ggcatttcct ccatctgatt tgtcagatag cactcaacac catatattat cttaatatcc
      601 ttgttcttgg ccgccttgta tacctcggga taagcctgca caacaccatg gtcggtaatg
      661 gcgatggcct tgtgtcccca tttcaatgct ctggggataa gctgttcagg tcttgtaacc
      721 gcatcctgcg gactcatgtt tgtatgcaga tgcagttcca ccctcttgac aggagcgttg
      781 tcctccttct caacaggctt gaatcttact atactccatg cactgattga cagttcatgt
      841 gtaaatgggt caaactccgt cttgcccatg acggtaaaat gcatgttctt tttaatagcc
      901 ccgtttactg cctttaggtc cttttgctca aaaaagaact ttacagtaac cgcacctgta
      961 ttgtcagcta tatggaatga gcatattgct ttttcatttg caatctcttt tatttcaata
     1021 ccaaccactc tgccccttat tgccacctgc cctgtgtata cgtcaacatc tttcatggga
     1081 gtgattggca tgtctatttc tcttccataa ataatttcat tacctttgga atcctttggc
     1141 aggcttccat cctcttgaaa attaagcttg tttcctcttc ttgaccctcc gccatttctt
     1201 cgtacaaagt ttccatttcc gccgtttgac gtgccattcg agccattgta cttctgctga
     1261 cgcagctttt cttcattgcg cttaatactt tcctgaatag ccctgtcatg gtcctgggac
     1321 gtaaaatcca ccttgacaac ttcagtgttt attccttcga tctccacatc acaatctgaa
     1381 aaatctatct ctacatgctc gtcccacatc attttaattt gatctgcaat gaatttgtct
     1441 acttgttttt ccttaagaat gccagcaacc tttttgtcca aatcaacaac aagtctttta
     1501 tttctaagct cgcatttgct tccttccaaa agggcccaaa cggaattgtt ctcttccttg
     1561 aaatatgaaa gcatattttc ccaaaagtct tctccattaa taacagattc catgcatgcc
     1621 ttgcatgaaa gctggagtat gactttgtcg tatcctatct tcttgcatat cttcttcttt
     1681 atttccttta tcttgttgta tgatacaaat gtatcagcaa cagccttgac tatgaacttt
     1741 ttgctgccgt gtacggcatt aacactttga ataactatat ttccgtccat gacttatcct
     1801 aactccctaa tcttttgtat gaattgttct gctattgaat cgcctttcaa tttgcctaca
     1861 atctttccgt tagaaaacaa aagtgcctcg tttctgccac ctgcaatgcc aatatcagca
     1921 tacgaggctt ctccaggtcc gttcacagca caacccatca ccgcaacggt aatatgcctc
     1981 tttatctccc cctttccgtc catttcaaaa agcgcctttt ccacctcttc tgcaatacct
     2041 gtcaaatcaa gctctgttct tccgcaagta ggacaggaaa tgaaagtaaa attccttcct
     2101 cccagttcca atgcagcaag aaggctcttt cccacttcta tttccctgac aggatccccg
     2161 gtaagagaga ctcttatggt atcaccaatc ccctgcatga gaattgcgcc gatgccagca
     2221 gacgacttga tggttccaga caggaaggtt cccgcctcgg ttacacccac atgaagagga
     2281 taattcctca tgttggccat cagcatatat gaatcgacgc acaatttaat gtttgtagct
     2341 tttagcgata ttacaatatc gtaaaaatca agaccctcca aaatctttat gtgttccatg
     2401 gcgctttcca ccaaagcttc ctttgaaagc ccgtatcttt cctttatact tttgtccaga
     2461 gagcctgaat taattccaat ccttatggga atgtttcttt cctttgccgc ctttacaacc
     2521 tgttcaaccc ggtctctatc cccaatattg ccagggttga ttcttacctt gtctactcca
     2581 tttttaatac actccaaagc aagcctgtag tcaaaatgta tgtcgctgac caaaggaaca
     2641 ttaatctgtt tctttatctc ttttacagca attgcggctt ccaaatcggg aactgcgacc
     2701 cttacaatgt cgcagcccgc ttcctcaagc atccttatct gttttacggt gctctttacg
     2761 tccctggtat cggtattagt cattgattgc acactgattc tattaccgcc acctatataa
     2821 acgttacctg cacgaacaac tcgggtttca ttccgtagat acaattgaat ccccaatcct
     2881 tcttatctta tcagtcttag tatgtcattt cctgctacaa ccaatgcaag caacagaagc
     2941 agtccgattc cgacatatga gaattttgct tctgtctccg gggaaatctt ttttcctcct
     3001 cttaaaagct caataagtat aaacaatagt tttccgccat caaggcctgg cactataagc
     3061 agattgaaaa ctgcaaggtt tgcgcttatt aaagcagtca tattaataag tgcaagcagt
     3121 tttggaagcc aggaagcatc ctgttgaacc acctcattga caatacccgt cattcctatc
     3181 ggacttgcta cggcgtcaag tccgagctgg ccggttatga gccagaagat tgcgtagaaa
     3241 acagacttaa tcaaggagaa aatatagagc atggtgtttc ctaatatgcc aaagaagttg
     3301 cctttctcga caccaaaacg gaaacctata tagctttttg ctccattttc aagcttttct
     3361 ctttctatag tgtgtttcct gaccgtgcca tccgccttct ttatttcaat ctcacttgtc
     3421 atatccttgt caaccatttc aaaaatacgg taatcattcg gagtatggac tctctttccg
     3481 tcataggata tgattctgtc tcctacctca aagccaagtt cctgagcagg atatgtgcct
     3541 tcctcagcga ccaacacttc ggaaaccttg tttgtttcat aaccaaaaaa agaaaacata
     3601 atggcaataa tgaggatggc taaaagcaga ttcataagag ctccggcaag caaaattacc
     3661 gccctggcat accagggttt ggagtcaaag gaagtcgaac tctgtaccgt ctcctcttct
     3721 ccttccataa ggcaaaatcc gcctatcggc aaagctctta tcgaccatct tgttccggtt
     3781 ttcttgctca ctcttgaaaa gatctttgga cccatgaata tggaaaactc ttcgaccttg
     3841 accttaaaaa gtctggccgc gaaaaaatgc ccaaactcgt gtacgactac gagtatgctg
     3901 agcataaaga gcattatcaa cacactacct aacataccca tttataattc ctcctgctgt
     3961 tcttctggct tccaggtcac accttagtat atcctccaaa tcaggacttg gcaaactttt
     4021 atgtccttcc ataaccttgc cgacaatctc ggaaatcatc atgaatggaa tttcacctct
     4081 cagaaagctt tctacacata cttcatttgc tgcattcatg acacaaggca tggtgcctcc
     4141 tatccttgca gcttcatatg catacttaag gcaagggaat ctgtccatat ccggct
//
LOCUS       DAONVY010000162         4180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15021_length_4180_cov_3.447758, whole genome shotgun sequence.
ACCESSION   DAONVY010000162 DAONVY010000000
VERSION     DAONVY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15021_length_4180_cov_3.447758"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLI11_05430"
     CDS             <1..969
                     /locus_tag="PLI11_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPZ52340.1"
                     /translation="RDDHPNSYWFQEDYIREKDIMVGDIVEVMKAGDIIPAIVSVDKD
                     ARNDGYERKGFVMPENCPVCGSDVVREAGEAAARCIGIECPARVFRGIVHFASRDAMN
                     IENLGPSLIQRLIDEGFISNIPDLYTLHEKKDKLVRLERMGEKSVSRLLENIENSKKN
                     EIYRLIFGLGIRHIGVANSKELAKRFKSMESLQKATVEDLVAINDIGYTTAVSIVEFF
                     RMPQTIHTLELLKEAGVNMEDRQEGSKEDIRFEGLNFVLTGTLPSLKRAEAKKLIEDR
                     GGKCLSSVSKNTDYVLAGEDAGSKLQKAKELGITIIDEEAFLKMLE"
     gene            1038..2486
                     /gene="gltX"
                     /locus_tag="PLI11_05435"
     CDS             1038..2486
                     /gene="gltX"
                     /locus_tag="PLI11_05435"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPZ52341.1"
                     /translation="MRTRFAPSPTGYMHIGNLRTALYEYLIAKSNGGKFILRIEDTDQ
                     ERYVEGAMDAIFNTLEMTGIKYDEGPYVGGDYGPYIQSERLHLYKKYAQELVDKGEAY
                     YCFCTKERLDSLKKSAAELGDALNYDRHCFYLDKEEVEKNLRNNVPHVIRQKMPTEGT
                     TTFHDVVYGDITIENKTLEDQVLLKSDGYPTYNFANVVDDHLMKITHVVRGNEYLSST
                     PKYNLLYSAFGWEIPTYIHLPPVMKDQHTKLSKRHGDASFQDLVERGYLPKAILNYIA
                     LLGWSPKDTEEIFTLEELEKSFNLDGLSKSPAIFDIEKLTWMNGEHIRRMDLDEFYDV
                     ARPYIEKGTNSSLTEEQMKKIAGLIQKRTEVLTEIPGKISFFAELPDYDIQIYVHKKM
                     KTDLENSLTYLKEGFDYLKEAQDFTSNSLYEGIKELAAKLGCKNSQILWPMRTALSGV
                     EVSPGGATEIAEILGKEESLKRMEYGISKLGG"
     gene            2488..4173
                     /locus_tag="PLI11_05440"
     CDS             2488..4173
                     /locus_tag="PLI11_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPZ52342.1"
                     /translation="MDSEFVTTNFIHEMVANDIKEGGRYYGKRVHTRFPPEPNGYLHI
                     GHAKAICVDFGTAEKFGGLCNLRFDDTNPEKEDEEYVEAIKEDIKWLGFKWDGIYYAS
                     DYFPRMYECAKKLIKKGLAYVCDLSPDEIREYRGTLTEAGKNSPYRDRSIEENLDLFE
                     RMKNGEFEEGSRVLRAKIDMASPNIVMRDPVIYRILYATHHRTGDEWCIYPMYDFAHP
                     LEDAFEGITHSLCDIGFKNNRPLYDWFIRECGPFDPEPCQTEFARLNLTHTVMSKRLL
                     RRLVENNVVSGWDDPRMPTLRGLRRRGYTPKAIRNFIEAVGVSTTLSVVDHALLEHYV
                     REDLNRVAPRGMAVLDPIKLVIDNYEDGLVEEFEAEVNPENPEMGKRKVEFSKVLYIE
                     REDFMIEPVKGYFRLYPGNEVRLKYAYYVKCTSYETDENGNVTVVHATYDPESRGGWL
                     PTRKVKGTIHWVSAHNAVQSEVRLYDKLFLQEDPLDDSDGIDFVDKANPDSLQVVTNA
                     YVEPFLANSEKNAHFQFLRLGYFSTDNDHTPEKPVFNRTVSLKDSWAKKAKSK"
BASE COUNT         1342 a          720 c         1079 g         1039 t
ORIGIN      
        1 cgtgacgatc accccaactc ctactggttc caggaagact acatcaggga aaaagatatt
       61 atggttggcg atattgtgga ggtaatgaag gctggtgaca ttattcctgc aatagtttct
      121 gtggacaagg atgccagaaa tgacggttat gagagaaaag gatttgttat gccggaaaac
      181 tgtcccgtgt gcggctctga tgttgtcagg gaggctgggg aagctgcagc aaggtgtatc
      241 ggaattgagt gtccggcaag agtatttaga gggattgtac attttgcatc cagggatgct
      301 atgaatattg aaaatctagg accaagtctc atacaaaggc tcatagacga gggctttatc
      361 tccaacatac cggatttgta tactcttcat gaaaaaaaag ataaacttgt tcgtctggag
      421 agaatgggtg agaagtccgt cagtaggctt ttggagaata ttgaaaacag caagaagaac
      481 gaaatataca ggcttatttt cggtctggga ataagacata taggtgtggc aaattccaag
      541 gagctcgcaa aacgtttcaa atcgatggag agtttgcaaa aggcaaccgt ggaggatttg
      601 gttgctatta atgatatagg gtatacaacg gctgtaagca ttgttgagtt tttcaggatg
      661 cctcagacaa ttcatactct cgaactcttg aaggaagcgg gagtcaacat ggaagacagg
      721 caagaagggt caaaggaaga cataaggttt gaagggttga acttcgtgtt aacggggacg
      781 cttcccagtc tgaagagggc agaggcgaag aaattaatag aggacagagg cggtaaatgt
      841 ttaagcagtg tttcgaaaaa tactgattat gtgcttgctg gagaggatgc cggaagcaag
      901 ctgcagaagg caaaggagct tggtatcact attattgacg aggaagcctt cttgaaaatg
      961 cttgagtaaa gcttgttgac acggttcatt agtgattata taattttaag gatatatgta
     1021 attattggag ggaagacatg aggacaagat ttgccccaag tccaacgggg tacatgcata
     1081 taggcaattt gaggacggct ctttatgagt atcttattgc aaaatcaaac ggaggaaagt
     1141 tcatattaag aatagaggac acggaccagg aaagatacgt ggaaggcgcc atggatgcca
     1201 tcttcaatac acttgaaatg acaggtatca aatacgacga aggtccttat gtgggtggag
     1261 attacggacc gtatatccaa agtgaaaggc tgcatttgta taaaaagtat gctcaggaat
     1321 tggtggacaa gggagaggca tactactgct tctgcacaaa ggaaaggctc gactctctaa
     1381 agaagagtgc tgccgaactt ggcgatgctt taaactatga caggcactgc ttttatttgg
     1441 acaaggaaga agtggagaaa aacctcagga acaatgttcc gcatgtaatc aggcagaaga
     1501 tgcccaccga aggaacaaca acctttcacg atgtggtata tggcgacatt acaattgaga
     1561 acaagacttt ggaggatcag gtgcttttaa aatccgatgg atatccaaca tacaactttg
     1621 caaatgtagt ggatgaccat ctgatgaaga ttacgcatgt tgtaagggga aacgaatatc
     1681 tttcatcaac acccaaatac aacctgctgt atagtgcttt tggatgggag atacccacat
     1741 acattcatct tcctcccgtc atgaaagacc agcataccaa actttcaaaa agacatgggg
     1801 atgcttcttt tcaggatttg gttgaaagag gttatctgcc caaggcgata ctaaactata
     1861 tagcactttt gggatggagt ccaaaagata ctgaggaaat cttcacactt gaggagcttg
     1921 aaaaatcttt taatctggac ggactaagca agtcgccagc cattttcgat attgaaaaac
     1981 tgacttggat gaatggcgaa catataagaa ggatggacct ggatgagttt tatgacgttg
     2041 caagaccata tatcgaaaaa ggcaccaatt cttcgttgac agaagagcag atgaagaaga
     2101 ttgccggact tatacaaaag agaacggaag tgctgacaga aataccgggg aagatatcgt
     2161 tctttgcaga attacccgat tatgatattc agatatatgt gcataaaaaa atgaagaccg
     2221 atttggagaa ctctttgact tacctgaaag agggattcga ctacctgaaa gaagcgcaag
     2281 attttacatc aaacagcttg tatgaaggaa tcaaggagct ggctgcaaaa ttaggatgta
     2341 agaacagtca gattctctgg cccatgagaa ctgctttgag tggagttgag gtaagtccgg
     2401 gaggagcaac ggaaattgca gagatactgg gaaaagaaga atcgctaaaa agaatggaat
     2461 acggtattag caaactggga ggttaaaatg gatagcgaat ttgtaacaac caactttatt
     2521 catgaaatgg tggccaacga cataaaagaa ggtggcagat attacggtaa aagggttcac
     2581 acaagatttc ctcccgaacc caacggatat ttgcacatag gacatgcaaa ggcgatttgt
     2641 gtagactttg ggactgccga aaagtttggc ggactttgca acctgcgttt tgacgacacc
     2701 aatccggaaa aagaggatga agagtatgtc gaggccatta aagaggatat taaatggctg
     2761 ggattcaaat gggatggcat ttactacgca tccgattatt tccccaggat gtacgagtgt
     2821 gctaaaaaac tcataaagaa gggactggca tacgtatgcg atttgagtcc tgatgaaata
     2881 agggagtata ggggaacttt gaccgaagct ggcaaaaaca gtccataccg ggacagaagc
     2941 atagaggaga atcttgacct gttcgagcgc atgaagaatg gtgaatttga agaagggtca
     3001 agggtattga gagccaagat agatatggct tctccaaaca tcgtgatgag ggatcctgtt
     3061 atttacagga tattgtatgc aacacaccac agaacaggtg acgaatggtg catatatccc
     3121 atgtatgact ttgcgcatcc tcttgaggac gcatttgaag gaataactca ttccttgtgt
     3181 gatataggtt tcaagaacaa cagacctctt tatgactggt ttatcaggga gtgcggtcct
     3241 tttgatcccg agccatgtca gaccgagttt gcaagactta atctcactca tactgttatg
     3301 agcaaaagac tgcttagaag actggttgag aacaatgtag tgtcgggttg ggatgaccct
     3361 agaatgccaa cccttcgcgg attgagaaga aggggctaca cacccaaggc cataaggaac
     3421 tttattgagg cagtgggcgt ttcaactact ttgagtgttg ttgaccatgc tctacttgag
     3481 cattacgtta gggaagacct taatagagtt gcgccaagag gaatggctgt tctcgatcca
     3541 atcaagctgg ttatagacaa ctatgaagac ggtttggttg aggaatttga agcggaggtc
     3601 aatccagaga atcctgagat gggaaaaagg aaggtagagt tctcaaaggt tctttatatt
     3661 gaaagggaag actttatgat agaacctgta aaaggatatt tcagacttta tcctggcaat
     3721 gaggtaagac tcaagtatgc atattatgta aaatgtacat cctatgaaac cgatgagaat
     3781 ggcaatgtga ccgtcgtgca cgcaacttat gaccctgagt ccagaggtgg atggctccca
     3841 acaagaaagg tgaagggtac catacactgg gtgtctgcac acaatgcggt acagagcgag
     3901 gtgcgtttgt acgacaagtt gttcctgcag gaagacccct tggatgattc cgatggaatt
     3961 gattttgtag acaaggcgaa tccggattca ttgcaggtag tgacaaacgc atatgtggag
     4021 ccttttcttg ccaatagtga aaagaatgca cacttccagt tcctaagact tggatatttc
     4081 agcacagaca atgatcatac accggagaaa cctgtgttca ataggactgt gtctttgaag
     4141 gattcctggg ctaagaaggc aaagtccaaa taatggtagg
//
LOCUS       DAONVY010000163         4171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15068_length_4171_cov_3.301749, whole genome shotgun sequence.
ACCESSION   DAONVY010000163 DAONVY010000000
VERSION     DAONVY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15068_length_4171_cov_3.301749"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLI11_05445"
     CDS             complement(<1..778)
                     /locus_tag="PLI11_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPZ52343.1"
                     /translation="MRYEIDHDIHIHSHLSLCSNDPIQTKENILAYAIRNNLKYICLT
                     DHFWDENIPRANEFYEPQNFEHIKKALPLPESDSVKFYFGCETEMDKNYRLGISEERF
                     DSFDFVIIPTTHLHMMSFTIDEKDTSLERRADLYVARLDRLLDMDIPFHKIGIAHLTC
                     PLMAPNNKDDHLKVLDLIPDKVFKELFSKIAKKKAGFELNFSFFQYEGESLERVLRPY
                     RIAKECNCKFYLGSDAHHPQGFDYAIKNFSAIIDALDLKES"
     gene            complement(1372..2631)
                     /locus_tag="PLI11_05450"
     CDS             complement(1372..2631)
                     /locus_tag="PLI11_05450"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPZ52344.1"
                     /translation="MTKPYCFERYIKTETCIPKSIKKVSEPRIYITVNEAIEDFSAFK
                     SIILYAYSGRQYWESPSLTFNDLFSSIDKKLHNMDSAKISIPELCDWYHTCLKGMKIV
                     DNHLTIYTLYNGIFRFGAEYAAYFTDVTVEKDFNSYRVIQSNDKQINVGSIIDVNKDN
                     LFKTLSPSDKEYYLVGIRSWTPVETISVECNGKEIQLPLHKCRASANKFTGRRNHFIH
                     SDVDGICVVTSNTFELNENYTFDMAKTLGKNLQSKKALIWNLSDNSGGTSDYADYFIR
                     GLNGYSECNTNVAILHSNYTDASYNNTPYYKWDYIIDEGYDYSKSMYKGFLYVIMNSR
                     TASSGETAIGYAKSVEKCLTFGENSYGCGVFGELKYYQLPYSKTTMAVPSKIFLGDFE
                     EGKGYTPDYWVDSIDVLSEVLKWLKYK"
     gene            complement(2945..3808)
                     /gene="ispE"
                     /locus_tag="PLI11_05455"
     CDS             complement(2945..3808)
                     /gene="ispE"
                     /locus_tag="PLI11_05455"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPZ52345.1"
                     /translation="MGNIVVKSKAKVNLSLDILGLRDDGYHEVLMIVQTIPLYDMITI
                     TTEKSIAPSIELTCNKKTLPTDSRNLAYKAAKMLMDKAGINDRIKISIKKRIPVGGGL
                     GGGSSNAASVLNGLNTLYKLGIPTEELAEMSKTLGSDVPALVYGGCVLAKGTGTDVSP
                     LPSLKKGYMLLVNPGVFVSTEKVYHKYDTMHIPKDAHPDTNQLIKFLIQGDLHAFAKN
                     MKNVLEYPVLESKSKIRNLKQEMMQTGALGTMMTGSGSSIFSLFDDIKKAYAALEHFR
                     KQHYFAVLINL"
     gene            complement(3880..4152)
                     /locus_tag="PLI11_05460"
     CDS             complement(3880..4152)
                     /locus_tag="PLI11_05460"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HPZ52346.1"
                     /translation="MIAILKNNAFRIVDRIREMDREKKEKKRIEMEYALLQEELYKTN
                     VQIRSAYNNFNNTTDKDCISYYLFLIKALESRYALLLKRAKDIDYA"
BASE COUNT         1264 a          755 c          731 g         1421 t
ORIGIN      
        1 cagattcctt aagatccagt gcatcaataa tagcactgaa gttctttatt gcataatcaa
       61 aaccttgtgg atggtgggca tcactgccca gatagaactt acaattacat tccttggcta
      121 tcctgtatgg tctaagcact ctctccaagg attctccttc atattgaaaa aaggagaagt
      181 ttagctcaaa acctgctttc tttttagcaa tttttgaaaa cagttcttta aagactttgt
      241 ctggtatgag gtccaatacc ttcaagtgat cgtccttgtt gtttggggcc attaagggac
      301 aggtcagatg ggcaattcca atcttgtgga aaggtatatc catatcaagt agtctgtcaa
      361 gcctggcaac ataaaggtct gcgcgccttt caagtgatgt atccttttcg tctattgtga
      421 aggacatcat gtgcagatgg gtagttggga tgataacaaa gtcgaaagag tcaaaccttt
      481 cttccgatat gccaagtctg tagttcttat ccatctctgt ttcacagcca aaatagaatt
      541 taactgaatc actttcaggc aaaggaagag cctttttaat atgctcaaaa ttctgcggct
      601 cataaaactc attagctctg ggtatatttt catcccaaaa atgatcagtc aaacaaatgt
      661 atttcagatt attccttatt gcatacgcaa gaatattctc ttttgtctgt attgggtcat
      721 tggaacacaa tgagagatga gagtgtatat gtatatcatg gtctatttca tatctcatac
      781 taagcctccc aatcaataat taagattata atacctccaa tactgtcaaa tgtcaattga
      841 ggcatccatc aagaaaaact acctatttgt ataattttgt ataatgttag taataaaata
      901 gtttgaatgc aaatcacagc ctatcgtctc attcatagag gatgagaggc tgtttttgcg
      961 taactatata tatatatata agttaagatg ggtaatgtac atataataaa agcatgtgtt
     1021 aatataaact gaaaaaatat aaggaattga taagatgaca caggaagagt tttcaaataa
     1081 atttatagca catctgaata tgcagcagaa agaagcggtc ggggaagtat tataaccatg
     1141 acatacacgg taaccgccac cgaggaaatg aagcagcgtt ttgcactgaa gtttggatga
     1201 gggaaagccg tgaagttata cagcctttga ttttaaagat gcctactgag aacctcaata
     1261 ggcattaaac cgttcagtat tcattttatt tgggaaaggt gatccatttt aatatacaat
     1321 gcacaccgta gtcaaaaaga tgcacaacac accccctttt aaattagtat tttatttata
     1381 cttaagccat tttagaactt cgctcagaac atcaatgcta tcaacccagt aatccggtgt
     1441 atatccctta ccttcttcaa aatcacctaa aaatattttg cttggtacgg ccattgtagt
     1501 tttactataa ggtaattgat aatattttaa ttcgccaaac accccacaac cgtaggagtt
     1561 ttctccgaat gtcaaacatt tttctacact ttttgcatat cctattgccg tttctccaga
     1621 agaagctgtc cgactattca taataacata tagaaaacct ttgtacatgg atttcgaata
     1681 atcataaccc tcatcaataa tgtaatccca tttataatat ggtgtgttat tatacgatgc
     1741 atcagtataa ttggaatgta aaatggcgac attagtatta cactctgagt aaccattaag
     1801 acctctgatg aaataatctg cataatcaga tgttcctcca ctattgtctg atagattcca
     1861 tattaatgct tttttgcttt gtaaattctt tcctaacgtt tttgccatat caaatgtata
     1921 attctcgtta agttcgaatg tgttggatgt aactacacag ataccatcaa cgtcactatg
     1981 aatgaagtgg tttcgtctac ctgtaaattt attggccgag gcacgacatt tatgtaatgg
     2041 caactggatt tctttgccgt tacattctac agaaatagtt tccaccggag tccaactgcg
     2101 tattcctaca agataatatt ctttatcaga aggagataaa gttttgaata gattatcctt
     2161 atttacatca attatactgc cgacatttat ttgtttgtca ttactctgta taacacggta
     2221 tgagttaaaa tctttttcaa cggtaacgtc ggtaaaatat gcagcatatt cagcaccaaa
     2281 tctaaagata ccattataca aggtatagat agtcaagtga ttatctacga ttttcattcc
     2341 ttttaggcat gtatgatacc aatcgcagag ttcaggtatc gaaattttag ctgaatccat
     2401 attatgcagc ttcttgtcaa tggaagaaaa aagatcatta aatgttagtg aggggctttc
     2461 ccaatactgt cttccagaat aagcatataa gataattgac ttgaaagcgg agaaatcttc
     2521 aatggcttca ttaaccgtaa tataaatacg tggttcggaa acttttttga tactttttgg
     2581 tatgcatgtt tctgttttaa tatatctttc aaagcaatag ggttttgtca ttttcaaatc
     2641 atctccgatt tataagcttt aatgaaaaaa attatatcat aattaacaat aaagaaaagt
     2701 aattctagat gccgcttgtt atgatacaag tggtataatc tgcacctcgg atcttgtggt
     2761 caaatgttat tcggtccctt aggtataaat attattgaaa tatcattgct ttgtattata
     2821 atgcaatatt tggaaggagg gtcgagtagt gaaagaaaaa atggttaaag aagttactgt
     2881 ttattctaat aattgtcata ctctctcccg taatcattgt gttgtaattt taccggagta
     2941 agctctataa atttatcaag acggcaaagt aatgttgttt tctaaagtgt tccagggctg
     3001 cgtaagcctt tttaatatca tcaaacagtg aaaagatgga tgagcctgaa ccggtcatca
     3061 tggtgccaag tgcaccggtc tgcatcattt cctgtttcag attcctaatt tttgacttgc
     3121 tttccaggac aggatattca agaacattct tcatgttttt agcaaaagca tgaagatccc
     3181 cctgtattaa gaactttatg agctgatttg tgtcagggtg tgcatctttt ggaatatgca
     3241 tggtgtcgta cttgtgatac actttttcag ttgatacaaa aacacccgga ttaacaagaa
     3301 gcatatagcc ttttttaaga gaagggagag gggagacatc cgtacctgtg ccttttgcaa
     3361 gaacgcaacc tccatataca agagcgggga catcggaacc caatgttttt gacatttcag
     3421 ccagctcttc cgttggtatg cccaacttgt acaatgtgtt aaggccattt aggactgaag
     3481 cggcatttga gctgcctcca cctagacctc ctccgacggg tattcttttt tttatactaa
     3541 tcttgattct atcgttgatt cctgccttgt ccatcagcat ttttgcggct ttgtaagcca
     3601 gatttcttga atctgtagga agtgttttct tgttgcatgt aagttcgata gatggggcta
     3661 tcgacttttc tgttgttatg gttatcatat cgtaaagagg aattgtttgc actatcatta
     3721 acacttcgtg atagccatca tctcttaatc caagtatgtc cagtgaaaga tttacttttg
     3781 cttttgattt tacgactatg ttgcccatac aatactcctg acatttttca tatactttat
     3841 ctctttcaat tatacaacct attgtatgct gtttcaagat tatgcataat cgatatcttt
     3901 tgcccgtttc agaagaagtg catatcttga ttcaagcgct tttatgagaa atagatagta
     3961 ggagatgcaa tccttgtcag tagtgttgtt aaaattattg tacgcagaac gtatctgaac
     4021 attcgtcttg tatagttctt cttgaaggag tgcatactcc atctcaatac gcttcttttc
     4081 ttttttctct ctatccattt cccttattct atccactatc ctgaaggcgt tgttttttaa
     4141 aattgcaatc attttttatc cctctttata a
//
LOCUS       DAONVY010000164         4161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15109_length_4161_cov_2.868729, whole genome shotgun sequence.
ACCESSION   DAONVY010000164 DAONVY010000000
VERSION     DAONVY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15109_length_4161_cov_2.868729"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLI11_05465"
     CDS             complement(<1..204)
                     /locus_tag="PLI11_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52347.1"
                     /translation="MVITVELFHTLKKYNKERFKDDENSFELSIDGRDEILLDELVSL
                     LNIPDEEVGFATINSVKHNWNDPI"
     gene            complement(206..1984)
                     /locus_tag="PLI11_05470"
     CDS             complement(206..1984)
                     /locus_tag="PLI11_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ52348.1"
                     /translation="MFYKDYIRVLNINLSTGRIRVDKRKDLRAYLGGVGVASKLLQEN
                     MNPDLPPLHEDQPIIFAIGAASSVFPVITKTVAMFVSPLTGELGESYAGGRLAFTMLH
                     AGYDAIVITGKSQKPCYVTVTQTGVEIKDARAFWGMDSDKVGSYIRNREHGSGKRSII
                     RIGPAGENLCSYASVCVDTYRHFGRLGLGAVFGSKLMKAMQVTGDLPMPIPNFKDYFK
                     TYSKLYKQVTETEVMAKYHDAGTPINVEPLSAMGGLPTRNLTSGTFEHAENISGEAFA
                     KNNLIRKMACTGCPVGCIHIGQFRREFDHGYEYEAISVGYDYELIYALGSFLGVDNTD
                     GVLYVIDAVERNGLDAMSCGVVLGWACEALERGIITEEQTILPLKMGDYVNLAKAVDY
                     IAQGVNQLYTDMGKGVKYVAKKYGGYDFGMQIAGNEMPGYHTGYGSLVGALVGARHSH
                     LCNAGYSYDQSNRDKELDKEKLVDYLISEEIERCLLNSLIICLFARKVYSRENIRMVL
                     KCVGLDYTDETLTDIAKRILRTKLEIKKSLGFAIQDVKLPKRFFETPTTTGKLDQYMA
                     NEIAEEYARRCEEILAEEDESIKEFV"
     gene            complement(1974..2393)
                     /locus_tag="PLI11_05475"
     CDS             complement(1974..2393)
                     /locus_tag="PLI11_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPZ52349.1"
                     /translation="MARALRTDGMRKCIGCFSCMTVCAAVNKHNFSISKSAIKIKTNG
                     GLKHGFYATVCHACKDDRACLEACPTGALVERKGGGVLLNKDMCTGCKKCVDACIVGA
                     VFFDEEENLPIICKHCGSCTRFCPHQCLTMEEVDDVL"
     gene            2555..3283
                     /locus_tag="PLI11_05480"
     CDS             2555..3283
                     /locus_tag="PLI11_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPZ52350.1"
                     /translation="MSTHKSSKRYERQMNLQSLPDNFQDLLRNSKVLILGAGGLGCAA
                     SIYVAAAGVGKITVVDRDTINEPDLNRQILYTPEDIGKKKAKTCVERLHKFNPDIECV
                     GLAKEINDKLLKDLVPKHDIVMDLCDNYSTRIKIAEACQKYGRQCVVAAVNGLEGFVM
                     VTDKGSACFGCLYPDEVKLSVSPPRVLGAAAGNVGVLAAMRCILTLCNVGKVGVMYCL
                     DFINMIIDEIEITKSTNCHVCCKA"
     gene            3355..>4161
                     /locus_tag="PLI11_05485"
     CDS             3355..>4161
                     /locus_tag="PLI11_05485"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPZ52351.1"
                     /translation="MNYRELKIFAGNSNIPLARSIAKRLGRDLGKCTVGKFSDGETSI
                     DIAEAVRGDDVFIVQSTSRPVNDHLVELLILIDAMKRASAGRITAVIPYFGYARQDRK
                     AKRRDPISAKLVANMITCAGADRVLSMDLHTAQIEGFFDVPVDHLSGAPILADYFMKT
                     FGDEEFVVVAPDVGGVARARKFAQKLDASPLAIIDKRRQAANECEIMNIIGDVKGKHC
                     IMIDDIIDTAGTVCKGAELLMEKGAKTVYACCTHGVFSGNAIENINNSAIK"
BASE COUNT         1164 a          871 c          915 g         1211 t
ORIGIN      
        1 aatcggatcg ttccagttgt gtttgacact gtttatggtt gcaaatccaa cttcttcatc
       61 aggaatgttc aataacgaaa caagttcatc caataggatt tcatctcttc cgtcaattga
      121 gagttcaaaa ctgttctcgt cgtccttgaa cctttccttg ttgtactttt tcaatgtgtg
      181 aaacagctca acagttataa ccatcttaga caaactcctt tattgactca tcttcttcag
      241 ccaaaatttc ctcgcaacgc cttgcatatt cttctgcaat ctcgtttgcc atatactgat
      301 caagtttgcc tgttgttgtt ggtgtttcaa aaaatctctt gggaagcttt acatcctgta
      361 ttgcaaagcc caaggatttc ttaatctcta gctttgttct caggatacgc tttgcaatat
      421 ctgtcagtgt ttcgtccgtg tagtcaaggc caacgcattt aaggaccatc ctgatatttt
      481 ccctgctgta tactttacgt gcaaacaggc atatgattaa agaattcagc aggcagcgct
      541 cgatttcctc gctaataagg tagtctacga gtttttcctt gtcaagctcc ttatccctgt
      601 tggactggtc atatgaatag cctgcattac agaggtgaga atgtcttgcg cctaccaatg
      661 cgcctacaag agagccgtag cccgtgtggt aaccaggcat ttcgttacca gcaatctgca
      721 tgccaaaatc ataaccgcca tacttcttgg ctacgtattt tactcccttg cccatgtcgg
      781 tatagagctg attgacacct tgtgcaatgt agtcgacagc tttagcaagg tttacataat
      841 cgcccatctt caaaggcaga atggtttgct cttctgtaat gatgcccctt tcaagagctt
      901 cacaagccca tcccagaaca acaccgcagg acatggcgtc aagcccgttt ctttcaacag
      961 catcgataac gtataaaact ccatccgtat tatcaacacc aaggaatgag ccaagtgcat
     1021 aaattagttc gtagtcgtat ccgaccgaaa tcgcttcgta ctcatagccg tggtcaaatt
     1081 cacgtctgaa ctgtcctatg tgtatgcatc caacaggaca tcctgtgcat gccatttttc
     1141 taatcaggtt gttcttcgca aaggcctctc ctgaaatatt ttcagcatgc tcaaaagttc
     1201 cagaggtaag gtttcgggtc ggaagacctc ccatggcgct caatggctct acgtttatgg
     1261 gagtgccggc atcatgatat tttgccatta cttctgtttc ggttacctgt ttgtacagtt
     1321 tggagtaggt cttgaaatag tccttgaaat tgggtattgg catgggaaga tcgcctgtaa
     1381 cctgcattgc tttcataagc ttgctgccaa aaacggcacc caggcccagt cttccaaaat
     1441 gtctgtatgt gtcaacgcaa acacttgcat aggagcacag attttcacct gcaggaccta
     1501 tgcggatgat ggaacgcttt ccgcttccat gctccctgtt tctgatatat gagcctacct
     1561 tgtcggagtc cattccccaa aaagcgcgtg cgtcctttat ttccactcct gtttgtgtaa
     1621 ctgtaacata acaaggtttt tgagatttac ctgtaattac tatggcgtca tagcctgcat
     1681 gcagcattgt gaaggcgagt ctgccacctg cataggactc acccagttct cctgttagag
     1741 gagaaacaaa catggctaca gtcttggtta tgactggaaa tacagatgaa gctgcgccaa
     1801 ttgcaaaaat tataggttga tcctcatgaa ggggaggcaa atcaggattc atgttttcct
     1861 gtaggagttt tgaggcaact cccacacctc ccaaataggc tcttagatcc tttctcttgt
     1921 caacccttat gcggcctgtt gagagattta tattcaaaac tcttatgtaa tctttataaa
     1981 acatcgtcga cttcctccat tgtaagacat tgatgtggac agaatcttgt gcaggatcca
     2041 cagtgtttgc agattattgg caaattctct tcctcgtcaa aaaacaccgc accaactatg
     2101 caagcatcaa cacacttttt gcatccggta cacatatcct tgttaagcag gacaccgcct
     2161 cctttacgtt caaccaacgc acctgtagga caagcttcaa gacatgctct gtcatccttg
     2221 catgcgtggc atacagttgc atagaatccg tgtttcaagc ctccgtttgt ctttattttt
     2281 attgcacttt ttgaaatgga gaagttgtgt ttgttgacag ctgcacatac ggtcatgcat
     2341 gaaaagcagc caatgcactt cctcattccg tctgttctaa gcgctcttgc cacttaaatg
     2401 acttcctttc taaaaaaatt gtacagtatc tatcatatca tatattttat gtatttcaat
     2461 atactttgtt aaaaaataat caatttctac atgtccaatt ttatttgtat tattgaggga
     2521 aaattagtat actaataata taggggtgaa cagaatgagt acacacaaat caagcaaaag
     2581 atatgaaagg cagatgaatt tacagagtct tcccgacaac tttcaggact tattgcgtaa
     2641 tagcaaggtg ttgattctgg gagcaggagg cttgggctgt gcagcctcca tttatgtcgc
     2701 tgcagctggt gttggaaaaa taacggtggt ggacagggac actataaacg agcccgactt
     2761 gaacagacag atactctata caccggagga cataggaaaa aagaaagcca aaacatgtgt
     2821 ggagaggcta cataaattca acccggatat agaatgtgtg ggattggcaa aggagataaa
     2881 tgacaaacta ctaaaggacc ttgttccgaa gcatgatatt gtcatggatt tatgcgacaa
     2941 ctattccacg agaataaaaa tagctgaagc atgtcaaaaa tatggtaggc aatgtgtggt
     3001 tgcagctgta aatggacttg aaggatttgt gatggttaca gacaaaggtt ctgcatgttt
     3061 tggttgcctt tatcctgacg aagtaaagct ttcagtcagc ccgccgaggg ttttgggagc
     3121 agcagcagga aatgtgggag ttctagctgc catgcgatgc atactgactt tatgtaatgt
     3181 tggaaaggtc ggtgtcatgt attgtcttga ttttataaac atgattattg acgagataga
     3241 gataacgaaa agcaccaatt gtcatgtttg ctgcaaggct tgacgccttg ttttttagtc
     3301 ttaaaagtgg gataatatat atctcaccgt aaagttgata aagcggaggt tgttatgaat
     3361 tatagagaat tgaagatatt tgcagggaac tcgaacatac ctttggccag gtcgattgcc
     3421 aaaagacttg gaagagacct ggggaaatgt acggtgggaa aattcagcga tggagagacc
     3481 agcatagata ttgcagaagc ggtgagagga gatgacgtct ttatcgtaca atctacctca
     3541 agacctgtaa acgatcatct ggttgaactt cttattctta ttgacgcaat gaagagagca
     3601 tctgcgggaa ggattactgc ggtaataccc tattttggct acgcacgcca ggacagaaag
     3661 gcaaagagaa gggaccccat atcagcaaag ctggttgcaa atatgattac ctgtgcaggt
     3721 gccgacaggg tactttccat ggatctgcat acagcacaga ttgaaggttt ttttgatgtg
     3781 cctgtggatc atctgtctgg tgctccaata ctggcagact acttcatgaa aacctttggt
     3841 gatgaggaat ttgtagttgt agccccggat gtgggaggcg tggcaagggc aaggaagttt
     3901 gcccagaagc ttgatgcatc gcctcttgca attatagaca agcgtcgtca ggcggccaat
     3961 gaatgtgaga ttatgaatat aattggagat gtaaagggaa aacactgcat aatgatagac
     4021 gatataattg acacggcagg cactgtatgc aaaggtgccg aacttttgat ggagaaggga
     4081 gcgaagacgg tatacgcatg ctgtactcac ggtgtgtttt caggaaacgc aattgagaat
     4141 ataaataatt ctgcgataaa g
//
LOCUS       DAONVY010000165         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15254_length_4128_cov_4.572796, whole genome shotgun sequence.
ACCESSION   DAONVY010000165 DAONVY010000000
VERSION     DAONVY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15254_length_4128_cov_4.572796"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLI11_05490"
     CDS             <1..210
                     /locus_tag="PLI11_05490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPZ52352.1"
                     /translation="WYIIIDHGGGISTLYAHARELVAKAGDKVERGQVIAKVGTTGTS
                     TGNHLHFEVRVNGTPVNPLNYVVVP"
     gene            290..1657
                     /locus_tag="PLI11_05495"
     CDS             290..1657
                     /locus_tag="PLI11_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPZ52353.1"
                     /translation="MGKKFYLKDTKLTRKIKRIMKKKKEHQGIDVLEYREDDINETQS
                     PKKEYYFKLSASTLFFSAAIIIVITVFLSFFVADRLFYGGYFAEKSNKVSFDRTVDYR
                     KVAKFQDVLNFIRKNYYLEYDENELLEGALKGLVDALGDPYSNYFEPGSMDDYNDYIT
                     GTYRGIGVEVQEVENGLKVTNVFEDTPSWRVGIKKDDVITHINGKAATEMSSEERSNL
                     LGKEGNEVILTVVTSGGKTVQYDVKVEVIKKQTVFTKYYDNGIAYIQITQFDDDTGKE
                     FENVINEILKKDTKGLILDLRNNGGGFESQASIVADVILPEGVIAYSEDRNGKKIKEI
                     KSDKSELDLPIVMLINQNTASASELVAGAFRDFEKGVIIGVKSYGKALGQVSKTYEVD
                     GSGIVLTVARYFTPSGQCIHDIGIEPDIKVELAEEYKNISPDKLPFDFDDQLQRALME
                     IEKMI"
     gene            1654..2421
                     /locus_tag="PLI11_05500"
     CDS             1654..2421
                     /locus_tag="PLI11_05500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPZ52354.1"
                     /translation="MMYDSLAEFYNRFTKDINYKQLAEFVHEQFSGSGLFKGTDSPCV
                     VDLACGTGSLALELLKYAYDMIGVDLSYEMLEAARDAEYEVTGGSSILWICRDMSELE
                     LHGPVSGMVCITDGVNHITDKKKLDRFFKRVCKYLDVGGMFIFDILTERYFRDVVGSN
                     VYCESDDFESCIWESKYDVESRIMEYDITLYKLLDPEEDIYQRLDDVVVERAWTEEEL
                     YDSITKSKLKLKNVYSSVEKTNITNEDTRRYYILEKV"
     gene            2625..2825
                     /locus_tag="PLI11_05505"
     CDS             2625..2825
                     /locus_tag="PLI11_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPZ52355.1"
                     /translation="METGKVKWFNSDKGFGFIEQDNGNDVFVHYSAIEMDGFKTLEEG
                     MEVQFEIVEGSKGPQAANVRRV"
     gene            2857..3720
                     /gene="hslO"
                     /locus_tag="PLI11_05510"
     CDS             2857..3720
                     /gene="hslO"
                     /locus_tag="PLI11_05510"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPZ52356.1"
                     /translation="MLVNYLGANGQVRCVGVDSLEVLKNAARIHSTSNVATAAFGRTL
                     TGALILTRHLKNKDDILTLQIRGDGPLGGVVAVTTSDCKVRGYVINPDVTLPLKDGKL
                     DVGGAIGKGNITVVKDFGLKEPYVGSVPLLTGEIAEDLAYYLAVSEQIPSLISLGVLV
                     KGNEVLASGGFMIQPLPNTDEEIIVELEKRSMSFPPVSQLLYAGATIDDVLNDFMRGF
                     SMERLEEVETSYACNCSRDRIIGVIASLGKDGLAEIIEDDEGAEVKCHFCNTAYYFNT
                     DELKKINDELE"
     gene            3832..3907
                     /locus_tag="PLI11_05515"
     tRNA            3832..3907
                     /locus_tag="PLI11_05515"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3865..3867,aa:Val,seq:tac)
     gene            3913..3989
                     /locus_tag="PLI11_05520"
     tRNA            3913..3989
                     /locus_tag="PLI11_05520"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3947..3949,aa:Asp,seq:gtc)
     gene            3995..4070
                     /locus_tag="PLI11_05525"
     tRNA            3995..4070
                     /locus_tag="PLI11_05525"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4028..4030,aa:Phe,seq:gaa)
BASE COUNT         1275 a          648 c         1045 g         1160 t
ORIGIN      
        1 tggtacataa ttatagacca cggtggtgga atttcaacac tgtatgccca tgcaagagag
       61 ctggtcgcta aagcgggtga caaggtggaa cgtggccagg ttattgcaaa agtaggaacg
      121 actggaacat caacaggaaa ccatctgcat ttcgaagtaa gagttaatgg aacaccggtt
      181 aatccgctca attacgttgt ggttccttga ttttattgga ttattaaggt atactttacc
      241 cttgcaaggt atggtatacc ttattttttt tggaaggata gaggttaaaa tgggtaaaaa
      301 gttttatctt aaggatacta agttaacgag aaagataaaa agaataatga agaagaagaa
      361 ggaacatcag ggaattgatg ttctggaata cagggaagac gatattaacg aaactcagag
      421 tcctaaaaag gaatattatt ttaaacttag cgcttccaca ttatttttct cagcagcgat
      481 aataattgta ataaccgtat ttttatcatt ctttgttgca gacagacttt tctatggcgg
      541 atactttgct gaaaaaagca ataaggtttc atttgataga actgttgatt acagaaaagt
      601 agcaaaattt caggatgtgc ttaatttcat aaggaagaac tattacttgg agtatgatga
      661 aaacgaactg cttgaaggtg cattgaaagg ccttgttgac gctttgggag acccttactc
      721 caactatttt gaacctggta gcatggatga ttacaatgac tacataacag gtacctacag
      781 gggaatagga gtcgaagtcc aggaagttga aaacggactc aaggtaacaa atgtatttga
      841 ggatactcca tcatggagag tgggaataaa aaaggatgat gtcattactc atattaatgg
      901 taaagctgca actgaaatga gcagtgagga aagatcaaat cttctgggga aggaaggtaa
      961 tgaggtaatc cttacagtgg tgacttcagg tggcaaaaca gttcaatacg atgtaaaagt
     1021 tgaggttata aagaagcaga cggtttttac aaaatattat gataacggta ttgcatatat
     1081 ccagattacc cagtttgatg atgatacggg taaggagttt gagaatgtaa taaatgagat
     1141 attaaaaaag gataccaagg gacttatact ggatttgaga aacaacggag gaggatttga
     1201 atcccaagct tcaattgttg cagatgttat cctgcccgag ggagtaattg catactcaga
     1261 agacaggaac ggaaagaaga taaaggagat aaagtctgac aagagcgaac ttgatttacc
     1321 tatcgtaatg cttattaatc agaacacggc cagtgcctcc gagcttgttg ctggagcttt
     1381 ccgtgatttt gaaaagggtg tgattatagg ggtcaagtct tatggaaagg ctttgggtca
     1441 agtaagcaag acatacgagg tagacggaag cggaattgtc ttgactgttg cccgttattt
     1501 cacaccttca ggtcagtgca ttcatgatat tggcattgag cctgatatca aggttgaact
     1561 tgctgaagag tacaagaata tatcgccgga caagctgccc tttgattttg atgatcagct
     1621 tcaaagagct ttgatggaaa tagagaagat gatatgatgt acgattccct tgcagaattt
     1681 tacaacagat ttacaaaaga cataaactac aagcaactgg cggaatttgt ccatgagcag
     1741 ttttccgggt ccggcctgtt taaaggtacg gactctcctt gtgttgtgga ccttgcctgt
     1801 ggaacaggga gccttgcgtt ggagcttctc aagtatgcgt atgatatgat tggtgtcgac
     1861 ttatcctatg agatgctgga agccgcaaga gatgcagaat acgaggtcac aggtggaagc
     1921 agtatactat ggatatgtcg ggatatgagc gaacttgagc tgcatggtcc tgtttctggc
     1981 atggtttgta ttactgacgg agttaatcat attaccgata agaagaagct tgacaggttt
     2041 tttaaaagag tatgcaaata cctggatgtt ggcggcatgt tcatattcga tattcttacg
     2101 gagaggtatt ttagggatgt tgtagggagt aatgtatatt gtgaatcgga tgactttgaa
     2161 agctgtatat gggaatcaaa atatgatgta gagagcagga taatggaata cgatattaca
     2221 ttgtacaagc ttttggatcc cgaagaagac atatatcagc gtttggacga tgtggttgtg
     2281 gagagagcat ggactgaaga ggaactatat gactcgatta cgaagtcgaa gctgaagctg
     2341 aagaacgtat actcgtctgt tgaaaagaca aacataacga atgaagatac aaggcgttac
     2401 tatatactag aaaaagtttg attagaaatg tacatatcgt gtatactatc taaaagctta
     2461 tcaggctttt ggtcaaataa aaacgtaagg gttaacgcat tcttgagtgg tttgtgggtt
     2521 gacttacatt tccctgcgtg ttacacttat attgttgctt acgatattga tataaatata
     2581 tattggtaat gtgggcaaag ttaagtcagg aggagtttag aaatatggaa actggtaaag
     2641 taaaatggtt taattcggac aaaggctttg gctttattga gcaggataat ggaaacgatg
     2701 tctttgtcca ctattcggca attgaaatgg atggctttaa gactttggaa gaaggcatgg
     2761 aagttcaatt tgagatagtc gagggaagca aaggcccaca agccgcaaac gtaagaagag
     2821 tttgagtttt cctttggaac taaggtggaa taattaatgt tggtaaacta tctaggtgca
     2881 aacggtcagg tccgatgtgt aggagttgac tctcttgagg tgttgaaaaa tgctgcaaga
     2941 attcatagta caagcaatgt ggcgacagct gcgtttggac ggactttgac cggtgcatta
     3001 atattaacca gacatttaaa aaacaaggat gatattctca cattgcagat aagaggtgac
     3061 gggcctttgg gaggagttgt agcagttacg acatcagact gcaaggttcg tggctatgta
     3121 atcaatcccg atgtcacttt gcctcttaaa gacggtaagc tggatgtagg cggagctatt
     3181 ggtaaaggca acataaccgt cgttaaagac tttggtttaa aggaacccta tgtgggctcc
     3241 gtgccattgc ttacaggcga aatcgccgaa gatctagctt attatcttgc tgtgtctgag
     3301 cagataccat cactcatttc tctaggcgtg ttggtaaagg gtaacgaggt tcttgcgtct
     3361 ggaggattca tgatacaacc tctacccaat acggatgagg agataatagt cgagctggag
     3421 aaacgttcaa tgtcctttcc tccagtgtca cagcttttgt acgctggtgc aactattgat
     3481 gatgttctta acgatttcat gaggggtttc agtatggaaa ggcttgaaga agttgagaca
     3541 tcctatgcct gcaactgctc tagggacaga ataattggag tgatcgcaag tctaggcaaa
     3601 gatggacttg ctgaaattat cgaggatgac gaaggtgcag aagttaagtg tcatttctgt
     3661 aataccgcat attactttaa cactgatgag ttgaagaaaa taaatgacga gctggagtag
     3721 aaaaaattgg tgaaaaaata tttggttttt tcagaaaata gtgttgacaa tcatgaaaat
     3781 gtttagtata atgtattgcg tcgctgagat ttaagcggca ctggtacaag tgggagttta
     3841 gctcagctgg gagagcatct gccttacaag cagggggtca taggttcaag tcctataact
     3901 cccaccactt atggcccggt agttcagttg gttagaacgc tagcctgtca cgctagaggc
     3961 cgagggttcg agtcccttcc gggtcgccat ttttgcccag atagctcagt tggtagagca
     4021 gaggactgaa aatcctcgtg tcagtggttc gattccgctt ctgggcacca ggtgcgggtt
     4081 taactcagcg gtagagtgtc accttgccaa ggtgaaagtc gcgagttc
//
LOCUS       DAONVY010000166         4114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15320_length_4114_cov_3.152993, whole genome shotgun sequence.
ACCESSION   DAONVY010000166 DAONVY010000000
VERSION     DAONVY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15320_length_4114_cov_3.152993"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="PLI11_05530"
     CDS             complement(<1..1657)
                     /locus_tag="PLI11_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52357.1"
                     /translation="MARISDNVTPSLDLLDERASAFQRSVVFMRQAYPNMAYMTFDSN
                     EHRGANHLILAKFVVGIGEDNPVWLTYDECDDSTTCDNVPGGLFGTCHTKDGVRVETG
                     IYPLLVGRDTEKAEGACVIKVKCHGDKKVWLKFGNGNISFMHFSPNKDMMGPEIDCEK
                     GNAVIEDGVVVITRQERDEITCVRGNMEYERKLKGEGSYIVGKGASNELKVIVSFSKD
                     KDRALELSRLDPDEEFKKVQDYYADLLNNIYIKTPNPELDEAFAHALLNVEYSYLYPY
                     GWIESIHHWPTMWHMEHTAAEEWYGKPERTKIVLRAQMKNMFENGAIPDMCTTGKGRR
                     DWGGNNQFFFREVEHYLKMTLDMDFACEVEPYMDRVIKQTFDEYDPTYSGIIGWGTQI
                     GNQEDFESTPGKGAAPGSCGAEMLKTMAVVKGLLGKTEEADKYRRLSEYVIEKMKKTI
                     WLDDLGRYAWYEDVVGKKYLETTYHGIIYPIMHDQLDDFDMVSSIDHLEYRLTGPEGE
                     VYQTNHFGDHAYHWVPTWGMQCGSNMQPFATKAYSKLGMADSAI"
     gene            complement(1680..2867)
                     /locus_tag="PLI11_05535"
     CDS             complement(1680..2867)
                     /locus_tag="PLI11_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52358.1"
                     /translation="MMKENTIIKYFEENKGILNMVPVFVPRRFGNAGKRLRLHPHDYY
                     ALGTKRGSIKERWFSSVIAAMNGELAAQDEGMSYVNIDDNVENKILFKDFVDVLKENI
                     LGKELYNKFGTWPMYSKFFDYEGPLFHHLHLDFESAARVGKLGKPEAYYYPPQYNNYP
                     GKFPHTYFGFNPSVTKEEIRERLFNYEKEDIRITELSRAYRIELGTGWYTPPGVLHAP
                     GSYLTYEPQWNSDVNSVYENITDGEIYDSSFLYENCPEDKMGDVDYVLSLMDWEKNVD
                     PYYKEKYFRAPIVLEDNDDYTEKLIVYGNEYIEAKELTIKPGCEAVVKDETAYGAILV
                     QGYGYFGHHKCSTPSMLRFGQLSEDEFFVSYDAASKGVRIKNESKFEPLVMLKHYPYM
                     EKK"
     gene            complement(3108..3464)
                     /locus_tag="PLI11_05540"
     CDS             complement(3108..3464)
                     /locus_tag="PLI11_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52359.1"
                     /translation="MAFTENNQITVFPSGARTKEYWPDGAVTVDIPEGFGEMRKLVIV
                     DTGDSLYYYMNTAEEENVLILKIDINESEIKVFDANGEELCKAENFLDMDLGNHFKVF
                     SHFAYTFIDSIAVKAY"
     gene            complement(3703..3990)
                     /locus_tag="PLI11_05545"
     CDS             complement(3703..3990)
                     /locus_tag="PLI11_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52360.1"
                     /translation="MKKRVAIYILIVLCIFILSSCDQKIENKDDPTPQPTETATAGPT
                     VEPTEEPTPKPTKEPLPEGERGKPSEKSEVAYENEFIEEDLDGTLLVTTNI"
BASE COUNT         1047 a         1022 c          720 g         1325 t
ORIGIN      
        1 ttatagcgct gtcagccata cccagcttgc tgtatgcttt agtagcaaaa ggctgcatgt
       61 ttgaaccgca ctgcattccc caggtgggca cccagtggta tgcatggtcg ccaaagtggt
      121 tggtctgata aacctctcct tcaggacctg taagcctgta ttccaaatga tcaatggagg
      181 aaaccatatc aaagtcatca agctggtcgt gcatgattgg atagatgatg ccgtgatagg
      241 tggtttcaag atacttttta cctacaacat cctcatacca ggcgtatctt cccaaatcat
      301 caagccatat agttttcttc atcttctcaa taacatattc agataatctc cgatacttgt
      361 cagcttcctc tgttttaccc aacagccctt ttacaaccgc catcgtcttc agcatctcgg
      421 caccgcaact gccaggagca gctccctttc caggagttga ttcaaagtcc tcctgatttc
      481 caatctgtgt accccaacct ataatgccgc tgtaggttgg atcatactca tcaaaggtct
      541 gtttaatcac cctgtccata tagggttcga cctcgcatgc aaaatccata tccagagtca
      601 ttttcaggta gtgttccact tccctgaaga aaaactggtt gttgccgccc cagtcccttc
      661 tgccttttcc tgtggtgcac atatcgggaa tggctccatt ttcaaacatg ttcttcatct
      721 gggctctaag aacaattttg gttctttcag gctttccgta ccattcctcc gctgctgtat
      781 gctccatgtg ccacatggtt ggccagtgat ggatgctttc gatccatcca tatggataaa
      841 gatatgagta ttccacattc aaaagggcat gcgcaaaggc ttcatccagt tcaggatttg
      901 gtgtctttat gtatatgttg tttaagagat ctgcatagta atcctgcacc ttcttgaatt
      961 cttcgtcagg atccagtctt gacagctcaa gggccctatc cttgtctttt gagaaggaaa
     1021 caatgacttt cagctcattg gaagcaccct tcccaacaat gtagctgcct tcgcccttta
     1081 actttctttc atactccata ttacctctca cacaggtaat ttcatccctc tcctgcctgg
     1141 ttattaccac cacaccatct tcgattaccg cattgccctt ttcgcagtca atctcaggcc
     1201 ccatcatgtc cttgttgggt gaaaaatgca taaacgatat gttgccattg ccaaacttta
     1261 gccatacctt tttgtcacca tggcacttga ccttaataac gcaggcacct tcagcttttt
     1321 ccgtgtccct gccgacaaga agaggataaa tgcctgtttc aacccttacg ccgtcttttg
     1381 tatggcatgt tccaaacaat cctccaggca cgttgtcgca ggtggttgaa tcatcacatt
     1441 catcataagt cagccataca ggattgtctt caccaatacc cacaacaaac tttgccagta
     1501 tcaaatggtt ggcacccctg tgctcattgc tgtcaaaggt catgtaagcc atattgggat
     1561 atgcctgcct cataaataca acgcttcttt gaaaggcact cgccctttca tcaagcaaat
     1621 ccaatgaagg tgttacattg tctgaaattc tagccactgt taatctcctt tacaaatatt
     1681 tatttctttt ccatatatgg atagtgttta agcattacca atggttcaaa cttgctctcg
     1741 ttcttaatcc ttacaccctt tgaagcagca tcgtatgata caaagaattc gtcctcgctc
     1801 agctggccga atctaagcat tgaaggtgtg ctgcacttgt ggtgaccaaa gtatccgtat
     1861 ccctgaacaa gtattgctcc ataggccgtc tcgtctttaa caactgcctc gcatccaggc
     1921 ttgatggtaa gttcttttgc ttctatgtac tcgtttccgt aaactatcag tttctcagta
     1981 tagtcatcat tgtcttcaag cactattgga gcacggaagt atttctcctt gtaatatggg
     2041 tccacattct tctcccagtc cataagcgac aatacatagt ccacatctcc catcttgtct
     2101 tccggacagt tttcataaag gaatgaagaa tcgtaaatct ctccgtcagt tatgttctcg
     2161 tatacgctgt tgacatcgga attccactga ggttcatatg taagatacga gccgggtgca
     2221 tgcagaactc ctggaggtgt ataccagccg gttcccagct ctattctgta tgctcttgaa
     2281 agctcggtta tcctgatatc ctccttctca tagttaaaaa gcctttccct tatctcttcc
     2341 tttgtaacgg aaggattaaa gccaaagtat gtatgcggga atttccctgg atagttgttg
     2401 tactgaggag gatagtagta ggcttcgggc tttccaagct ttcctaccct tgcagcagat
     2461 tcaaagtcga ggtgcaaatg gtggaagagc ggtccttcat agtcaaagaa ctttgaatac
     2521 atgggccatg taccaaactt gttatatagc tcttttccca gaatattttc tttcaacaca
     2581 tctacaaaat ccttgaacaa tatcttgttt tcaacattgt catcaatatt tacatagctc
     2641 attccctcat cctgcgctgc cagttctcca ttcattgcag ctataacgga actgaaccat
     2701 ctttccttta tcgagcctct ttttgttccc agagcgtaat agtcgtgtgg atgcagtctt
     2761 agtcttttcc ctgcatttcc aaatcttctc gggacaaata cgggcaccat gttgagtata
     2821 cccttattct cttcgaaata ttttataatt gtattctctt tcatcatatc actctcctca
     2881 aattattcta acaatcatgt tttagtataa aaatagactg gacagacgtt tatttgcaca
     2941 aaacaaaagg aaattaataa tcatgtttta ataccccttt tcaacaagtc gtgggcatac
     3001 tcaccctcca ttgactctct gctagactcc tgactgcagt tgggtacaaa cctggacttt
     3061 catcctcatt caaaaatctc cactcaggca ttcatttatt gcctccacta gtacgctttt
     3121 acagcaatac tgtctatgaa tgtataggca aaatgactga ataccttgaa gtggtttccc
     3181 aaatccatat ccagaaagtt ttccgctttg cataattctt cgccatttgc gtcaaatacc
     3241 tttatttcac tctcattaat atcaattttc aatatcaaca cattctcttc ttctgccgtg
     3301 ttcatgtagt agtacaaact gtcaccggta tccacaatga ctagctttct catttctcca
     3361 aatccttctg gaatatcaac cgttacagcg ccgtccggcc aatattcttt ggttcttgca
     3421 ccacttggaa atactgtaat ttgattgttt tctgtaaaag ccacccatat tccgtcatcg
     3481 gtattggcta tagaacttac gtagtcgctt accctggccc caataattgt cgctatccaa
     3541 ggcgatgtta catcttcagg aaacctggat tcaaactcga tatggaactc ccactggtgt
     3601 gtatcgaatg tattgcagtc aatatcaggc gtcaggctat tccagccatc ccaagtcctt
     3661 ccgccgtctt cagagtttag aataagcttg ccatccttaa aactatatat tggttgttac
     3721 caacaatgtc ccgtccaaat cttcttctat gaattcgttt tcgtatgcaa cttccgactt
     3781 ctcagaaggc ttgccccttt ccccttcagg caaaggttct ttagtaggtt tgggagtcgg
     3841 ctcctccgtt ggttccacag tcgggcctgc agtagcagtt tccgtaggtt gtggtgttgg
     3901 gtcatccttg ttctcaattt tctgatcgca ggatgacagg ataaatatac aaagtacaat
     3961 caaaatatat atggctactc tctttttcat tatagtgcct cccttttttt ccgataataa
     4021 ttaccagcta caaatattat aatactgtgg aaacgtatct gaatatataa aatagactta
     4081 tatttgcaca attttaaaat ttttaactat ttgt
//
LOCUS       DAONVY010000167         4109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15341_length_4109_cov_3.671682, whole genome shotgun sequence.
ACCESSION   DAONVY010000167 DAONVY010000000
VERSION     DAONVY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15341_length_4109_cov_3.671682"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLI11_05550"
     CDS             <1..690
                     /locus_tag="PLI11_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52361.1"
                     /translation="NATILHAVSLGTIKHTYEKLVNGVDYTAKQAISYGFKRLLWYIL
                     YLFIVSFVTGFIYNIVSFVVALTFLSSFMDFGVIKVIIGAQVALALIVAVVYFMIRLY
                     FIPHAISIERIDCFKALSLSWNMTKGRVKKVFWPICFGVIFCAALPFVIRSLNNFLIF
                     NDPLVNRILAALIMSISSVLHPILYIHTTQLYIYLKHETGMIEFMNKLGDLVAGESDR
                     ITFGKDREVAG"
     gene            687..1385
                     /locus_tag="PLI11_05555"
     CDS             687..1385
                     /locus_tag="PLI11_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52362.1"
                     /translation="MNDRELAQRVIEEVLSQPEFQPEKVGEFKFLQKILRTIGEILQK
                     IMDFISDLIFEILSRVNLGSLGSKFLKLGPVARVIINVLAILIVGALVFFLVKLVLKI
                     IKSKRFRFKKEDISDELEEFTKTPSEPLRLALEFKRIKEFRLSFRYFFLALLINFNER
                     ELIRIQKFKTNRQYFRELSANDSEIAKASEPFFEAFYYIWYGKRDIGMKELLEWERLY
                     YDLSGGLEEGENDE"
     gene            1378..2418
                     /locus_tag="PLI11_05560"
     CDS             1378..2418
                     /locus_tag="PLI11_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52363.1"
                     /translation="MNNRLRFVVLGVVFVVLFFVAISLISDDDRQFENLSISAYSVKD
                     GGAKAFYLSVKEYGRRTNLYETEIFRKYARFTPDESLSVSLLPSLNYLIDDFEIEAIR
                     EHVKKGSSFLFFIEENEFNMYKDIVEDMEESINSTEEMYYKEYVFEDGKGGRIYLFPH
                     GDRILNGYMKKEHSYATDGLVLIGEIAKKGRYKTVAINEYYHVGVSASGGVDVYGMGV
                     TLLMVQIAITAILWMIYKGVRFGKVEPVYSTIKRNETENVHALGNLYKRVNSPQIVFD
                     VHMEALLGDVAHYLGYRQYNDTIRDEMLSDKKLCELGFDKLFSLYENKNMLKKGDIKK
                     YIEKIEKIRREL"
     gene            2420..3367
                     /locus_tag="PLI11_05565"
     CDS             2420..3367
                     /locus_tag="PLI11_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPZ52364.1"
                     /translation="MFVKEKSEQIINEVSKVLIGQDEFVRHLVVAFLTGGHVLMEGVP
                     GLGKTLSAKALAKTVDCEYKRVQFTPDLMPTDIIGTKVFDLKKTEFYLKKGPIFTNIL
                     LADEINRTPPKTQSALLEAMEEGHVTIDGETMELGDPFFVMATQNPIEYEGTYPLPEA
                     QLDRFLMKLNITYFSQEDENNLLKKYKEGFVSSHKEVESLETCLTYEELVTCREEISR
                     VICEDGVINYITSISNATRKSPAIILGGSPRSSISMLLCAKTTAAMNGRDFVTPEDVL
                     DIALPVLRHRVILKPEAEVDGLDPDTVLNAFIRKIEVPR"
     gene            3380..>4109
                     /locus_tag="PLI11_05570"
     CDS             3380..>4109
                     /locus_tag="PLI11_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPZ52365.1"
                     /translation="MTFTKRLIYLSLAGGVLVLVAGFAGWAFAVVAFTLVNLVLASLV
                     VVDIVQTPKPEVFEVHRELNTKLSLGAENDVEIIVRNNSPIPLLVSVTDSVPEHFKHT
                     LPMEAKWVPAHGQEIFKYQVTPMKRGEVCFENIYVRTLGIFGFIQRQTKIRSDEKYKV
                     YPNMKALTDYRISALNMNMFLHGIKKTRRNVQGGEFDSLREYVQSDPYNIINWAATAR
                     RHELIVNTYVPEKNQYIFIMIDSSR"
BASE COUNT         1236 a          700 c          974 g         1199 t
ORIGIN      
        1 aatgccacca tactgcatgc agtttctctg ggtactatca agcatacata tgaaaagctt
       61 gtaaacggag ttgactacac tgcaaaacag gcaatatcct acggtttcaa acgcctcctt
      121 tggtatattc tttatctgtt tattgtttcg tttgtcacag gttttatata caatattgtg
      181 tcttttgtag tggcgttaac ctttttgtcc agctttatgg attttggagt aataaaagta
      241 ataattggag cgcaggttgc ccttgcatta attgtcgctg tcgtttattt tatgattaga
      301 ctgtatttca ttcctcatgc aataagtatt gaaagaattg actgcttcaa ggcattaagt
      361 ctttcgtgga atatgacaaa gggaagggta aagaaagttt tttggcccat atgctttggt
      421 gtcattttct gcgctgccct gccttttgtc atacgttcac tgaataattt cctgatcttc
      481 aacgatcctt tggtgaaccg gattctggct gcactgatta tgtctatatc gagtgttttg
      541 catccaatac tttacatcca taccacacag ttgtatattt acctgaagca cgaaacggga
      601 atgatagaat ttatgaataa actgggtgat ttggttgcag gcgaaagtga tagaatcacg
      661 ttcggcaagg atagagaggt ggcaggatga acgatcgtga acttgcccag cgtgttattg
      721 aggaggtatt gtcccaaccg gaattccaac ctgaaaaagt tggagagttt aagtttttac
      781 aaaaaatcct taggactatt ggtgaaatac tgcaaaagat aatggacttt atttcagatt
      841 tgatttttga gattctgagc agagttaatc tgggttcact tggcagcaaa ttcctcaaac
      901 tgggtcctgt tgcaagggtg attattaatg ttttggcaat tctgattgtc ggggctttgg
      961 ttttctttct tgtcaagctt gttttgaaaa tcatcaaaag taaaagattc cggttcaaga
     1021 aggaagatat ttcagacgag cttgaggagt tcacgaaaac tcccagcgaa ccattgagac
     1081 tagcattgga attcaagaga ataaaagagt tcagattgtc tttcagatat ttcttcctgg
     1141 ctttgcttat caatttcaac gagcgggaac ttatacggat tcagaagttt aaaacaaaca
     1201 ggcagtattt cagggaactt tccgcaaatg actccgaaat cgcaaaggct agcgagcctt
     1261 tcttcgaagc cttttactac atttggtatg gaaaaaggga tataggcatg aaagagcttt
     1321 tggaatggga gcgtttgtac tacgaccttt cgggggggtt ggaggaaggt gagaacgatg
     1381 aataatcgat tgagatttgt tgtgcttggt gttgtatttg ttgttttgtt ttttgtggca
     1441 atttctctca tctccgatga tgaccggcag tttgaaaacc tttcaatttc cgcctattcg
     1501 gtaaaagatg gaggtgcgaa ggctttttat ctttcggtta aggaatacgg caggcgaacg
     1561 aatctgtatg agacggaaat tttcaggaaa tatgcaagat tcacccctga tgaaagcctc
     1621 agtgtttctc ttttgccttc acttaattat ttgattgatg attttgagat tgaagccata
     1681 agggaacatg ttaaaaaggg cagcagcttt ctgttcttca ttgaggaaaa tgaatttaat
     1741 atgtacaagg atattgtaga ggacatggaa gagtccataa actcaacgga agaaatgtac
     1801 tacaaagagt atgtgtttga ggatggaaaa ggcggaagga tctacttgtt tccccatgga
     1861 gatagaatat taaacggtta catgaagaag gaacattcgt atgcaacgga cggccttgtg
     1921 ctaatcgggg agattgcaaa aaaaggcagg tacaagactg ttgccattaa tgagtattac
     1981 catgttggag tgtcggcatc aggaggcgtc gatgtatacg gcatgggtgt tacacttttg
     2041 atggtgcaga ttgctattac tgcaatcctt tggatgattt acaagggcgt gcgctttgga
     2101 aaggttgaac ctgtttactc aaccattaaa aggaacgaaa cggaaaatgt tcatgctctc
     2161 ggtaatttat acaagagggt gaattcgccc cagattgtat ttgatgttca catggaggct
     2221 ttgcttgggg atgtggccca ttatctgggt tacagacagt acaatgacac aattagagat
     2281 gaaatgcttt cagacaaaaa actttgtgaa ttgggatttg acaagctgtt ttcgttgtat
     2341 gagaacaaaa atatgctgaa aaaaggagat attaaaaaat atattgaaaa aatagagaag
     2401 attagaaggg agctctaata tgtttgttaa ggaaaaatca gaacagatta taaatgaggt
     2461 atcgaaggtt cttattggac aggacgaatt tgtaaggcat cttgtggttg cttttttgac
     2521 tggcggacat gtgcttatgg aaggtgtgcc cggtcttggc aaaaccctgt ctgccaaagc
     2581 acttgcaaaa acggtggatt gcgagtacaa aagggtgcag tttactcctg acctaatgcc
     2641 aactgatatt atcggcacaa aagtatttga cttgaaaaag actgaattct atctaaaaaa
     2701 aggtcccatc tttaccaata tacttttggc tgatgagata aacaggacac ctcccaaaac
     2761 ccagtctgcc ttgctggagg ccatggaaga aggccatgta acaatagatg gagagacaat
     2821 ggaactggga gatccgttct ttgtaatggc aacacagaac cccatcgaat atgaaggaac
     2881 gtatccgctg ccagaagcac agctggacag attccttatg aaacttaata ttacgtattt
     2941 ttcccaggaa gatgagaaca accttcttaa gaaatacaag gagggttttg tgagttccca
     3001 taaggaagtt gaaagtcttg aaacatgcct gacttatgag gagctggtga cgtgcaggga
     3061 agagataagc agagttattt gtgaggacgg agttatcaac tacataactt caatatcaaa
     3121 tgcaaccaga aaatcacctg ccataatcct gggtggttcc ccaagaagct ccatcagcat
     3181 gcttttgtgt gcaaagacca cagccgccat gaatggaagg gattttgtaa ctcctgagga
     3241 tgtattggat atagctctgc cggtattgag acacagggta atattgaaac cggaggcgga
     3301 agttgacggt cttgatcctg atacggtttt gaatgcgttt attagaaaaa tagaggtgcc
     3361 tagatagagg agtaacaaaa tgacttttac caaaagactc atatatttat cacttgcagg
     3421 aggagttctt gtacttgttg ccgggtttgc aggctgggct tttgcagttg tggcattcac
     3481 actggtcaac ctggttttgg caagccttgt tgtggtggac attgtacaga ctccaaaacc
     3541 tgaagttttt gaggtccata gggagctgaa tacaaaatta agccttggtg ctgaaaatga
     3601 cgtggagata attgtaagga acaacagccc aatacctctt cttgtgtctg ttacggactc
     3661 cgtgcccgaa catttcaaac acaccttgcc catggaagcc aaatgggtac ccgcccacgg
     3721 ccaggagatt tttaaatacc aggtgacccc tatgaaaagg ggagaggtgt gttttgagaa
     3781 tatatatgta agaactttgg ggatatttgg tttcatacaa aggcagacca agataagaag
     3841 cgatgagaaa tacaaggttt accccaatat gaaagccctt accgattaca gaatcagcgc
     3901 tctcaacatg aacatgtttc ttcatggcat aaagaagact agaagaaatg tgcagggtgg
     3961 tgagtttgac agcttgagag aatatgtgca gtcggaccct tacaatatta tcaactgggc
     4021 agccacggca agaagacatg agcttattgt aaatacttac gttcctgaaa agaatcaata
     4081 tatatttatt atgatagatt ccagcaggg
//
LOCUS       DAONVY010000168         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15384_length_4101_cov_2.461691, whole genome shotgun sequence.
ACCESSION   DAONVY010000168 DAONVY010000000
VERSION     DAONVY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15384_length_4101_cov_2.461691"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1081
                     /locus_tag="PLI11_05575"
     CDS             65..1081
                     /locus_tag="PLI11_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPZ52366.1"
                     /translation="MFRRIMTMMLGLVFIFTMASCDVSSKKDGNDSEATPTPEPTSEA
                     EGKTILYQVVPEKLSLMMCYVIKTKNNKLIVIDGGIDGHGLEAPAYLLEELQEISGEE
                     HPVIDAWFITHAHYDHYYEFVKMVEQKNDKFTVNNVYFNFPSDEFIAKYEPNGEPYMD
                     RFRAAYNTLFGENAYQEYEGVQVGDIIEVDNVKFEILQVPDEKITANPINNSSLIFRM
                     EVEGQTVLFTGDAGAEAGSKLIREYKDTIQSDMVQMSHHGQAGLQKAHYVKINPKVCL
                     WPIPEWVWANTNKQFRTDETKKWMFEDLQVKHHYISGLYGTEYIEFPFDFDMEPKNNV
                     DYSY"
     gene            1616..>1988
                     /locus_tag="PLI11_05580"
     CDS             1616..>1988
                     /locus_tag="PLI11_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HPZ52367.1"
                     /translation="MIDVTLKDGSKIQVEKGTSVIEVAKMISHGLARNAVLAEVDGVV
                     TELGTTLDKDCTLNILTFDSEEGKKAYRHTTSHILAQAVMRLYKDVRLAIGPATENGF
                     YYDFDIDGTFTSEDLAKIEEEM"
     assembly_gap    1989..1998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1999..3549
                     /gene="thrS"
                     /locus_tag="PLI11_05585"
     CDS             <1999..3549
                     /gene="thrS"
                     /locus_tag="PLI11_05585"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPZ52368.1"
                     /translation="LPIERFVLPRDKAIEYMNELGETYKVELINDLPEDAEISFYRQG
                     EFVDLCSGPHLDNTGRVKAFKLIQTAGAYWRGDEKNKMLKRIYGTSFPDKKLLKEYLE
                     KLEEARKRDHNRLGRELELFTTVDYIGQGLPIMLPKGAKILQILQRFVEDEEEKRGYQ
                     ITKTPFMAKSDLYKISQHWYHYRDGMFIIGDPEKADEEEVLALRPMTCPFQFQAYLTK
                     TRSYRDLPIRYAETSTLFRNESSGEMHGLIRVRQFTISEGHLIVTPSQLEEEFRDCLD
                     LATFMLKTLGLHEDVFFRFSKWDPENKEKYIGSPEEWENVQSAMKTILDNSGLEYTEG
                     VGEAAFYGPKLDIQIRNVHGKEDTLITIQIDFQLAERFGMVYVDRDGTKKHPYIIHRT
                     SIGCYERTLALLIEKYAGAFPTWLAPVQVRVMPIVEKQADYCAEVVRKLKALGVRAEL
                     DDRNEKIGYRIREAQVEKIPYMLVAGDRELEEGKVSVRSRKQGEMGSIAVDEFCRMIK
                     EEIDEKRI"
     gene            3559..>4101
                     /locus_tag="PLI11_05590"
     CDS             3559..>4101
                     /locus_tag="PLI11_05590"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPZ52369.1"
                     /translation="MGILDRTFKETCRDILDNGVWVKDENIRARWEDGTPAYTIKKFG
                     VVNRYDLSKEFPLITIRPISFHKAVDEILWIWQKKSNNIKDLNSRIWDQWADENGSIG
                     KAYGYQMGIKHKYKEGEFDMVDRILYDLKNNPHSRRIMTNMYNFADLNEMALYPCAYS
                     MTFNVVDGRLCAILNQRSQDM"
BASE COUNT         1327 a          686 c         1005 g         1073 t
ORIGIN      
        1 ttaaatacat ggaagaagcc atggcatggt atgatagata aagctagttt tgtggggggt
       61 acatatgttt agaagaatta tgactatgat gttgggtctt gtgttcattt ttacaatggc
      121 gtcatgtgat gtgagcagca aaaaggatgg caatgactcg gaggctacac caacaccgga
      181 gccaaccagc gaggctgaag gaaagaccat cctttatcag gtggtgcctg aaaaacttag
      241 tcttatgatg tgttacgtta tcaagaccaa aaacaacaaa cttattgtta ttgacggggg
      301 aattgatggt catggtcttg aagcacctgc ttaccttttg gaagagctgc aagagataag
      361 tggtgaagag catccggtta ttgatgcctg gttcataacc catgcccatt acgaccatta
      421 ctatgagttt gtaaagatgg tggaacaaaa gaatgataag tttactgtaa acaatgtgta
      481 cttcaatttc ccgtctgatg agttcatagc caagtatgaa cccaatggtg aaccatacat
      541 ggacagattc agagctgcat ataacactct gttcggtgaa aatgcatatc aggaatatga
      601 gggtgtccag gttggcgaca tcattgaagt agacaatgtt aagtttgaaa ttcttcaggt
      661 gcctgatgag aaaattacag ccaatcccat aaacaactca tcccttatct tccgcatgga
      721 agttgagggc cagacagttc tgttcacagg tgatgcgggt gcggaagctg gctcaaagct
      781 aataagggag tataaagata ccatacaaag tgacatggtg cagatgtccc accatggtca
      841 ggctggtctt cagaaagcac attatgtgaa gattaatcca aaggtatgtt tgtggcctat
      901 acctgaatgg gtttgggcga acaccaacaa acagttcaga acagatgaga caaagaaatg
      961 gatgtttgag gatttgcaag tcaaacacca ttatatctca ggactatatg gtacggaata
     1021 tattgagttc ccgtttgatt ttgatatgga accaaagaat aatgtggatt acagttatta
     1081 ataaaggggc tgttgcaatt ggcaacagct ctttctaatt tacttcaggt tccttctcta
     1141 actccaattt tgcatactta tctttacttt aatctttgaa atattcaatc tttttctgca
     1201 actttgccat ttctttgggg cttagtgccg cactttttgc ggaaaacgac gcaagggcaa
     1261 ttgccgctac aaaagccaac aacaaaactc gttcgatggc tatactttga atacagtata
     1321 tgtttttttt aattaccgtt aaacttgtat ttatggataa aaataaacaa gtaaatgcat
     1381 tgaaatattc cagtatgtgg tatacaattt gatttagagg taaagaaaca tttggaaaac
     1441 aggaaaacag aaatatgtaa tgacggaaga cgctcaaggg agaagaacag agtaatgaga
     1501 taatcaccat aactttaaga ctagaagtca aattcttgat tttttgctga attgagcgta
     1561 caataaagag acaggtggat tgtagccacc cagtatattt taaagaggtt tcaatatgat
     1621 agacgtaact ttgaaagatg gatctaaaat acaagtagaa aaaggaacta gtgttattga
     1681 agttgctaaa atgattagtc atggacttgc aagaaatgca gtgcttgcag aagttgatgg
     1741 agttgttact gagcttggca caactctgga taaggactgc acattgaata ttttaacgtt
     1801 tgacagcgag gaagggaaaa aggcatacag acacacaacg tcccacatat tggcccaggc
     1861 agttatgaga ctgtataaag atgtacgctt ggcaataggt cctgctacgg agaatggatt
     1921 ttattacgac tttgatatag atggaacatt tacgagtgaa gatcttgcca aaatagaaga
     1981 ggaaatgann nnnnnnnnct ccctattgaa aggttcgttc tgcctaggga caaggctata
     2041 gagtatatga acgagctagg ggaaacatac aaagtggagc ttatcaatga tttgcctgag
     2101 gatgcggaaa tatcctttta cagacaggga gagtttgtgg atttgtgttc cgggccgcat
     2161 cttgataata caggaagagt gaaggctttc aagcttatcc agacagcagg agcttactgg
     2221 agaggtgatg aaaagaacaa gatgcttaag agaatctacg gaacaagctt tcctgacaag
     2281 aaacttttga aggagtatct tgagaagctt gaagaggcga gaaaaagaga ccataacaga
     2341 ttgggccgtg agttggagct gtttaccacc gtagattata ttggtcaagg actacctatt
     2401 atgctgccaa agggagctaa aatactgcag attctgcagc gttttgtgga agacgaagaa
     2461 gaaaagcgtg gttaccagat tacaaaaaca ccatttatgg caaagtctga tttatacaag
     2521 atatcacagc attggtacca ctaccgtgat ggcatgttca ttattggcga ccctgaaaag
     2581 gctgacgaag aggaagtact tgcactaagg ccaatgactt gtccattcca gtttcaggct
     2641 tacctaacaa agactagaag ttacagggac cttccaataa ggtatgctga gacttcgact
     2701 ttgtttagaa acgagtcttc cggtgaaatg catggactta tcagagtaag acagtttacc
     2761 atttcggagg gacatttgat agttactcct tcgcagcttg aagaggagtt cagagactgt
     2821 ctggatcttg caaccttcat gttgaagaca cttggactgc atgaggatgt gttcttcagg
     2881 ttctcaaaat gggatcctga gaacaaggag aaatatattg gaagtcctga ggagtgggag
     2941 aatgttcaaa gtgcaatgaa gaccatcctt gacaattcgg gcttggagta tactgaaggc
     3001 gtgggtgaag ctgcgttcta tggtccgaag cttgacattc agattaggaa cgtccacggc
     3061 aaggaagata cccttattac aatccagatc gacttccagc tggcagagag attcggaatg
     3121 gtctatgtgg acagggatgg aacaaagaaa cacccatata tcattcaccg tacttcaatt
     3181 ggatgctatg aaagaaccct tgctctcttg atagagaagt atgcaggtgc tttcccgaca
     3241 tggcttgctc cggtgcaggt aagagtcatg ccaattgttg aaaagcaggc ggactactgt
     3301 gcagaggttg taaggaagct taaggcgcta ggtgtaagag ccgagcttga tgacaggaat
     3361 gaaaagattg gctacaggat aagggaagca caggtggaga agattccata catgctggta
     3421 gctggggata gagagctgga ggaaggaaaa gttagtgtaa gatccagaaa gcaaggagaa
     3481 atgggcagta ttgcagttga tgaattctgc agaatgatta aagaagaaat agatgagaag
     3541 agaatatagg gggttgaaat gggaatactg gacaggacat tcaaggaaac atgcagggat
     3601 atactagata atggagtatg ggtaaaggat gagaatatta gagccagatg ggaagacggt
     3661 actcctgcat atacgataaa gaagtttgga gttgtaaata gatacgattt gtcaaaggaa
     3721 ttcccgctta ttactatccg ccctattagc ttccacaaag ctgtagatga aatactttgg
     3781 atttggcaga agaaatccaa caacataaag gatttgaaca gcagaatctg ggaccagtgg
     3841 gcggatgaaa acggctccat tggtaaagcc tacggatatc agatgggaat aaagcacaag
     3901 tacaaagaag gggagttcga catggtggac aggattcttt atgaccttaa gaacaatccc
     3961 cacagccgca gaatcatgac taatatgtac aattttgcgg accttaatga aatggcactt
     4021 tacccttgtg cttactccat gacttttaat gtggtggatg gaagactttg tgcaatactt
     4081 aaccagcgct cccaggatat g
//
LOCUS       DAONVY010000169         4084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15467_length_4084_cov_3.433606, whole genome shotgun sequence.
ACCESSION   DAONVY010000169 DAONVY010000000
VERSION     DAONVY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15467_length_4084_cov_3.433606"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLI11_05595"
     CDS             complement(<1..400)
                     /locus_tag="PLI11_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPZ52370.1"
                     /translation="MDYSKMLNLPKTDFPMRANLPQREPEYLKKWEEDKIYEKLIERD
                     RNNKKFVLHDGPPYANGDIHLGTALNKILKDIVVKYYSMKGYYSPYMPGWDTHGLPTE
                     QRAIKELNLRREEVGPVVFRNACRDFALKYL"
     gene            1186..1323
                     /locus_tag="PLI11_05600"
     CDS             1186..1323
                     /locus_tag="PLI11_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52371.1"
                     /translation="MVDNSKKRTLFRRVVFLKDNTAYYRFLSKHDPYGFDLTNPEDIY
                     I"
     gene            complement(1488..1862)
                     /locus_tag="PLI11_05605"
     CDS             complement(1488..1862)
                     /locus_tag="PLI11_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPZ52372.1"
                     /translation="MRFHHIGITTRDIEKSIEFYKVLGFKESKTWGEGKKRVVMLESD
                     EGAIVELFADGEDLLPEGAFRHLALKVDNTRELFEKIKVHGAEIVSEPRDMDLPSNPP
                     VPVTLAFFKGPDGEIIELFQER"
     gene            1984..2535
                     /locus_tag="PLI11_05610"
     CDS             1984..2535
                     /locus_tag="PLI11_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPZ52373.1"
                     /translation="MNGQTMCRKCQRRIKVHNAMRNLWEHHIFWTYNFINSMVQNLPD
                     AKYARQRLLQNPEDFERELVPYYSRYVSNLFATLLREHLVLAADMLSAILSGNPREAD
                     RLKRQWYQNAEDIARFLSEINQYWRRDEWKNMLFEHLKMVERMVELTLSENQKEKVFF
                     LDEMEKQALSMADYMTQGILSQF"
     gene            2599..3306
                     /locus_tag="PLI11_05615"
     CDS             2599..3306
                     /locus_tag="PLI11_05615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPZ52374.1"
                     /translation="MRLYIDSIGRDVDIDIEYKRTSRYLVRVTPDMNVRITCPLGAKT
                     FEINRVLDNAKPWIAQRIKKLEKSIILGNESLSSGSYIYLFGDRKTIDTRQGKPKVLL
                     EDDSMIVYVEDESKVKNTFDKWWKNLAEITFSDIINKQSRFFNDMCLDTPSISVKKMY
                     TRWGSCNKRLNKVNLNSYLLSLPYECIEYVIMHELAHLVHQNHKQEFYSLLVKYMPDW
                     KQRKKVIDDNFLLCKKS"
     gene            complement(3307..3921)
                     /locus_tag="PLI11_05620"
     CDS             complement(3307..3921)
                     /locus_tag="PLI11_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52375.1"
                     /translation="MDLAFKDRFADTALSLKKSENYCIYIVLSHSSSVISKAIYLYTR
                     DEYTHASISIDRNLEALYGFGRKWPRFPFIGCLRRELMDQGFYSLCKTVPGLILKFDV
                     TEEQYRRIKRLLSFYVKNKDLFKYNFLGFFGYMFKTEIRSKRRFTCSEFVAHLLQSAQ
                     VVKFDKPLNLIRPQTFTELGGQIVYKGDLKSFFKNLEKNNSLIV"
BASE COUNT         1270 a          827 c          693 g         1294 t
ORIGIN      
        1 ccagatattt caaggcaaaa tccctgcatg cgtttctaaa cactacaggt cccacttctt
       61 cccttctcag attaagttcc ttgattgctc tctgctctgt aggaagtcca tgagtgtccc
      121 atcccggcat gtatggtgag tagtatccct tcatgctgta gtatttgaca acaatgtcct
      181 tcaatatttt atttagtgcc gtacccaggt gaatatctcc atttgcataa ggagggccgt
      241 catgaagaac aaacttcttg ttgttcctgt ctctttctat taatttttca tatatcttgt
      301 cctcttccca tttcttcaaa tattccggtt ccctttgagg caaatttgct ctcattggaa
      361 aatcagtctt gggcaaattt agcattttgc tgtaatccat tttcttccct ccattaatgt
      421 ttatataaat tattgcctgt aattgccata catatgatta aaacacaaaa acacatgcca
      481 cacttgcatg ttctttcaat tatatttcat gctttttgca atgtcaattc ttttaattcc
      541 cttcaaaaca catcctccat tatgcaaaaa aaccccgtct catttgagac ggagattgtg
      601 tttacctacc gcaaccactc aaaatgcaga ttcaatcaaa tcctggcatg taacggggcc
      661 atccggaaaa cgcctaatag acagacatcg ttcagcgtat cagctcggga gggatgtgca
      721 ttcagtgaga tttccgctga tttccaccaa ccatcagctc tctattgggt catcaccgac
      781 gccaggctcc ttcatcgcct ttttttgttt tgattaatta tacaaaaagt ggctgttttt
      841 gtcaaacata tttcttttct attgtatcac tatatgcgaa ggtcttttta ttcaaatatt
      901 catattacac cacatgactg tcaacccttg ttgatttcat accattccag aattgtcgta
      961 atagcatagg ttccgtaatt gtttatgtag taaatttggt tatccttatt gtatactacc
     1021 tttttgcagc cttttgaaga tgtatccttg tactaataat caacgacctc cttgccgtta
     1081 taaatcatta tttcaccttc cgtttcaaaa cgtgcttttt taccatccct gcttgaggac
     1141 acaatcacag atatacttag cctatatctt tttaatggag gagttatggt ggataattca
     1201 aagaaaagaa cattattcag gcgagtcgtt tttttaaaag acaacaccgc atactacaga
     1261 ttcttgtcaa agcacgaccc atatggattt gatttgacaa acccggaaga tatatacata
     1321 tagcaaaaaa gaagagttcc ccaaatacat atataagttt ttagattctg atgaaaaatt
     1381 gagcgtgact cttgtaaata tattttacga tcaattatga ggatgcagtt accgaagaca
     1441 taaccgttta tgtgaataga taaacaaaga ggcattcgag cctcttttta tctttcttga
     1501 aacagttcaa ttatctctcc gtccggacct ttaaaaaacg caagagtaac aggaacagga
     1561 ggatttgaag gaagatccat atcccttggt tctgaaacaa tctcagcacc atgaactttt
     1621 atcttttcaa acaactccct tgtattgtcc actttaagtg ccagatgcct gaaagcacct
     1681 tcagggagca aatcctctcc atctgcgaaa agttcaacaa tagcgccttc gtcgctttcc
     1741 agcatcacaa ctcgcttctt gccctctccc cacgtctttg actctttgaa tcccaacact
     1801 ttataaaatt ctattgattt ttcaatatcc cttgtagtta taccgatatg atgaaacctc
     1861 atgtcttgct cctttcattt ttcctatatt ctacttcacg gcttccaagt tgtccatatg
     1921 tttttcactt caaacatgtc aaaaacatat tctgtattga tattagtttt aggagagatt
     1981 tgtatgaacg gtcaaacaat gtgtagaaaa tgccagcgaa ggataaaagt acacaatgca
     2041 atgagaaacc tatgggaaca ccacatattc tggacataca atttcataaa cagcatggtc
     2101 cagaatcttc ctgatgcaaa atatgcaaga caaaggcttc tgcaaaaccc ggaagacttt
     2161 gaaagagaac tagtaccgta ttattcaaga tacgtctcga atctttttgc aactcttttg
     2221 agagaacacc tggttctggc tgcagacatg ttgagtgcta ttctgtcagg caatccaagg
     2281 gaggcggaca gactgaaaag acaatggtac cagaatgcag aagacattgc cagattcttg
     2341 tctgagataa accagtactg gaggcgggac gaatggaaga acatgctgtt tgagcatttg
     2401 aaaatggtgg aaagaatggt tgaactaacc ctttcagaaa accaaaagga aaaggttttc
     2461 tttcttgatg aaatggaaaa gcaggctctt tccatggccg attacatgac tcaaggtatt
     2521 ctgtctcagt tttaaggttg ttgtttcttc atatgtatga tatattcttg tagaacaatt
     2581 tgtgagggtg tttgttaagt gagattgtat atagacagta taggcaggga cgtagatatt
     2641 gacattgagt acaaaaggac aagcaggtac cttgtgaggg ttactcctga catgaatgtt
     2701 aggataacat gtcctcttgg tgcaaaaact tttgaaataa acagagttct tgacaatgca
     2761 aaaccctgga ttgcacaaag aattaaaaaa cttgaaaaat ccattatcct cggaaacgag
     2821 tccctgtcct ccggcagtta catatatctc tttggagata gaaaaactat agatacaaga
     2881 caaggaaaac ccaaagtatt gctggaagat gattctatga ttgtctatgt ggaagacgaa
     2941 agcaaagtga aaaatacttt tgataaatgg tggaaaaacc ttgccgaaat aaccttttcg
     3001 gatattataa acaagcaaag cagattcttc aacgatatgt gtttagacac accttccatc
     3061 agtgtcaaaa aaatgtacac cagatgggga agctgcaata aaaggcttaa caaggtaaac
     3121 cttaactcat acctcctaag ccttccatat gaatgtatcg aatatgtaat tatgcacgaa
     3181 cttgcacatc tggttcatca aaaccacaag caggaatttt actccctgct tgtaaaatac
     3241 atgcccgatt ggaaacaaag aaaaaaagtt attgatgaca actttttgct ttgcaaaaag
     3301 agttaatcaa acaatcagac tgttgttctt ttcaagattt ttgaaaaaac ttttcagatc
     3361 ccctttatat acaatctgtc cacctagttc ggtaaatgtt tgaggtctaa tgaggtttaa
     3421 aggtttgtca aactttacaa cctgggcact ctgcaaaaga tgtgctacaa actcggagca
     3481 tgtaaatctt cttttgcttc taatctctgt tttaaacatg tacccaaaga agccaaggaa
     3541 attgtattta aataaatctt tattctttac ataaaagctc aacagccttt ttatgcgtct
     3601 gtactgttct tccgttacat caaatttaag tatcaggcct ggaacagttt tacataacga
     3661 ataaaaccct tgatccatga gctctcttct cagacagcct ataaaaggaa aacggggcca
     3721 cttcctgcca aagccatata gggcttccaa atttctatct attgatatgg atgcatgcgt
     3781 atactcatct cttgtataga ggtaaatcgc tttgctgatt acggaactcg aatggcttaa
     3841 tactatgtaa atgcagtaat tttcagattt tttcaaactt aacgccgtat cagcaaatct
     3901 gtctttaaac gctaaatcca tttatacatc accttgtttc tagtttgact ccctgtttat
     3961 tcaggataaa gggaattata taaaataaat attaagaatc tatgaacatt agcttttaaa
     4021 aaatttacca tataaaagag gagttttcaa gccccctctt ttatccgttt aacttttccc
     4081 tcag
//
LOCUS       DAONVY010000170         4075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15508_length_4075_cov_3.939303, whole genome shotgun sequence.
ACCESSION   DAONVY010000170 DAONVY010000000
VERSION     DAONVY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15508_length_4075_cov_3.939303"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLI11_05625"
     CDS             <1..319
                     /locus_tag="PLI11_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPZ52376.1"
                     /translation="HEDALFLSERKNRISPKTVEYTVKKYIKKANLDPKKYSTHKLRH
                     TAATLMYQHGDVDIRSLQEILGHESIATTEIYTHVSNKRLKQAVSSNPLANFRIDEDE
                     NES"
     gene            380..1339
                     /locus_tag="PLI11_05630"
     CDS             380..1339
                     /locus_tag="PLI11_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPZ52377.1"
                     /translation="MDNLRRFVIVITIFLFFATSVCGFLLALGYSGISKEKETTIDID
                     ADPYKPDDDQPTSFKGNILVIVGEKSRPETDLLFVVNYDSNHNQLSFLYIPKDLKYTD
                     PIIKETNSIGSYYSKNSAEKTATLVSSILDINIPYYINLNDNAFARMINMFEAVNFNM
                     PISIKYKDAYYDIDIKKNENVFDGTMALKLIQFYKTEDDTYTNTMLNYYNGKDTSRIR
                     MASNFLNAFVSQKFGEQYKDQYFDLFKLLLPECETNITESVLRSMTAKLDKINHDISR
                     SYMITGKETISTKYYIEFDNLFLDFTSGLNVSSRQVLDTRFVS"
     gene            1483..2655
                     /gene="nifS"
                     /locus_tag="PLI11_05635"
     CDS             1483..2655
                     /gene="nifS"
                     /locus_tag="PLI11_05635"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPZ52378.1"
                     /translation="MSEKRIYCDHSATTPLHEEVLNEMLPFFRTNFGNASSVYKEGRE
                     SRKAVELARDRVANAIGAEPNEIYFTSSGTESDNWVLRGISTKKGKHIITSSIEHPAV
                     LETCKDLEKKGYEVTYLPVDEDGLVSPQEVKDAIRDDTILVSIMFANNEIGTIQPIAE
                     IGEVCKNMGVPFHTDAVQAVGNVPINVKEMNMDFLSMSAHKFYGPKGVGALYVRKGLR
                     LANFLTGGHQERGRRASTENVPGIVGLGKAIEIATNDIGNKAEYLRKMRDRAIERIEK
                     EIPYVKLNGHREKRLPGHVNFSFRYIEGESILLMLDLNGVAASSGSACSSGSLDPSHV
                     LLAIGLSHEVAHGSLRLTFGRENTLEEIDYVVDKLKEIVERLRFMSPLYEGVEKEV"
     gene            2661..3116
                     /gene="nifU"
                     /locus_tag="PLI11_05640"
     CDS             2661..3116
                     /gene="nifU"
                     /locus_tag="PLI11_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPZ52379.1"
                     /translation="MYSEKVIDHFSNPRNVGEIPDADGVGEVGNAKCGDIMKMYLKIV
                     DDVIIDVKFRTFGCGAAVATSSIATEMILGKTVDEALQLTNKAVADALGGLPPVKMHC
                     SLLAEEAVKAAILDYKKRRGLFIDDSLAVCEGCCDACYHKQDDEEVHEE"
     gene            3143..>4075
                     /gene="tyrS"
                     /locus_tag="PLI11_05645"
     CDS             3143..>4075
                     /gene="tyrS"
                     /locus_tag="PLI11_05645"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPZ52380.1"
                     /translation="MSNVYDTLVEREIIAQCTNEEKVREILENENVSFYIGFDPTADS
                     LHIGHFVQIMVMVHMQRAGHRPIALVGGGTGKIGDPSGKTDMRKMLTDEELAHNVESI
                     KSQLSRFLDFGEGKAIMVNNADWLLKLNYVEFLRDIGVHFSVNRMLAAECFKQRLEKG
                     LNFIEFNYMLMQSYDFLKLYQDYECRFEFGGDDQWSNIIGGVELIRRVEGAEAFGVTF
                     KLLTNSEGVKMGKTVKGAVWLDKEKTPPYELYQYLRNVDDADVIKCLKMLTFVPLEEI
                     REMEKLEGSEINQAQVRLAYEVTKMVHGEEEAEKA"
BASE COUNT         1368 a          674 c          937 g         1096 t
ORIGIN      
        1 tcatgaagat gccttgtttc tcagcgaaag aaaaaacaga atcagcccca aaactgtaga
       61 gtatactgtt aaaaagtaca taaaaaaggc caatctcgat ccaaaaaaat attcaactca
      121 caaactaaga catactgctg ccacacttat gtaccagcat ggagatgtgg acatacgctc
      181 gctacaggag atactggggc atgaaagcat agcaacaact gagatatata ctcatgtaag
      241 caacaagagg ctgaaacagg cagtatcaag caatcctctt gcgaacttca ggattgatga
      301 agatgaaaac gagagttgac ggaaatcagg aaatatatat actatactag tacctaatat
      361 tttgaaaggt gaaagaagaa tggacaatct aagaagattt gttattgtaa ttactatatt
      421 tcttttcttt gccacttccg tatgtggttt tttgcttgca ttgggctata gtggtatatc
      481 gaaagaaaag gaaaccacta ttgatataga tgcagatccg tataaaccgg atgacgatca
      541 acctacaagt ttcaaaggca acattcttgt aatcgtaggg gagaagtcaa gaccggaaac
      601 ggatttgttg tttgttgtaa attacgactc aaatcacaac caactgtctt ttttatacat
      661 tcctaaagac ttgaaatata ctgatccaat aataaaggaa acaaactcaa ttggaagcta
      721 ctattcaaag aatagtgccg aaaagacggc aacgttggtt tcctcgattc tggatataaa
      781 cattccatat tacattaatc ttaacgacaa tgcttttgcc aggatgatta acatgtttga
      841 agcagtaaac ttcaacatgc ccatcagtat aaagtacaag gatgcatatt atgatataga
      901 tattaagaag aatgaaaacg tttttgatgg aacaatggca ctaaaactta tccagtttta
      961 caagactgag gatgatacgt acacaaacac aatgcttaat tattacaatg gtaaggatac
     1021 aagcaggatt agaatggcat cgaatttctt aaatgctttt gtatcacaga aattcggcga
     1081 gcaatacaaa gatcagtatt ttgatttgtt caagctgctt ttgcctgaat gcgagacaaa
     1141 tattactgaa agtgttttga ggtcaatgac tgcaaagctt gacaagataa atcatgatat
     1201 atcaagatcc tatatgataa caggaaagga aactattagt acaaaatatt atattgagtt
     1261 tgataatctg ttcttggatt tcacttcagg actgaatgtc agctcaagac aggtccttga
     1321 tacaagattt gtatcataat taggatataa taggaataaa atgcaactct ttgttaaggg
     1381 ttgcattttc tttaattgaa aattctgtat caaagtgcta taatgaagaa taagtatttt
     1441 attgaaatac ttaaatccac aacagaaaag aggtaagcaa tcttgagtga aaaacgaata
     1501 tattgcgatc actcagcaac cacaccgtta catgaggaag ttttgaacga aatgcttcca
     1561 ttttttagaa caaactttgg aaatgcttcg agtgtttaca aagaagggcg tgagtccaga
     1621 aaagcagtag agcttgcaag agacagagtg gcgaatgcaa tcggtgcaga accgaatgaa
     1681 atatatttta caagctccgg aacggagtct gacaattggg tgctgagagg gatcagcacg
     1741 aagaaaggca aacatatcat aacatcttcc atagaacatc ctgcagttct tgaaacatgc
     1801 aaggacttgg agaagaaagg gtatgaagta acatacctgc ctgttgatga agatgggctt
     1861 gtatcaccac aagaggtcaa ggatgcgata agggatgaca ccattttggt aagcattatg
     1921 tttgccaaca atgagattgg aacaatccag cctatagctg aaatagggga agtatgcaaa
     1981 aacatgggtg ttcctttcca taccgacgct gttcaggctg taggaaatgt tcccattaat
     2041 gtaaaggaaa tgaatatgga tttcttgtca atgtctgccc ataaatttta cggaccaaag
     2101 ggagttggcg ctctttatgt aaggaaggga cttagacttg ccaacttttt aaccggcgga
     2161 caccaggaaa gaggtagaag agcttctact gagaacgttc ctggtattgt ggggctcggc
     2221 aaggctattg aaattgctac aaacgatata gggaataagg ctgaatatct ccgtaaaatg
     2281 agagatagag ctatagaacg tattgaaaag gaaatacctt acgtcaagct gaatggtcac
     2341 agggagaaga gattgcccgg gcatgtcaat ttttcattca gatatattga aggtgaatca
     2401 attcttctaa tgcttgatct gaatggtgtt gccgcttcca gcgggtctgc gtgctcttca
     2461 ggttcacttg acccatcaca tgtattactg gctataggtc tttcccatga agttgctcat
     2521 ggctccttga gacttacatt tggccgtgag aatactttgg aagagattga ttacgttgta
     2581 gacaaattga aagaaatagt tgaaagatta aggtttatgt cacctttata tgaaggagtc
     2641 gaaaaggagg tataaaaatg atgtacagcg aaaaggtcat tgatcatttt tcaaacccac
     2701 gcaatgtagg cgagataccc gatgctgacg gagttggaga agttggcaac gcaaaatgcg
     2761 gggacattat gaaaatgtat ttgaaaatag ttgatgatgt aataatagat gtcaagttca
     2821 gaacctttgg ctgcggtgct gctgttgcta ccagcagtat tgctacagaa atgattctgg
     2881 gcaagactgt tgacgaggct cttcagctta caaacaaggc tgttgccgat gcactgggtg
     2941 gattgccacc agtcaagatg cactgcagtc tgcttgctga ggaagctgta aaggctgcta
     3001 ttcttgacta taaaaagaga agaggtcttt tcatagatga ttcgctggct gtttgtgaag
     3061 gatgttgcga tgcatgctac cacaagcagg acgatgagga agtacacgag gaataacata
     3121 aacaattgaa cggaggtttg aaatgtcaaa cgtttatgac actttggtcg agcgagagat
     3181 tattgcacaa tgtacaaacg aggaaaaggt aagagagatt ctcgaaaatg agaatgtatc
     3241 tttctatatt ggttttgatc ctactgctga cagtctccac atagggcatt ttgttcaaat
     3301 tatggtaatg gttcatatgc agagggcagg gcataggccg attgctcttg ttggcggagg
     3361 aaccggtaaa atcggggatc cgtccggaaa aaccgacatg agaaaaatgc tcactgacga
     3421 ggagcttgcc cataatgtgg aaagcattaa gagccagttg tcaagatttc ttgactttgg
     3481 tgaaggaaag gcaataatgg ttaataatgc cgactggtta cttaagttga actatgtgga
     3541 atttttgaga gatattggcg ttcatttttc ggtcaataga atgctggctg cagaatgctt
     3601 caaacaaaga ctggagaagg gactcaattt catagagttc aactacatgc tcatgcagag
     3661 ctacgacttt ttgaaactgt atcaggatta cgaatgcagg tttgaatttg gaggagacga
     3721 ccaatggtcg aatatcatag gtggcgttga acttatacgc agggttgaag gcgctgaagc
     3781 atttggcgtc acattcaagc tgctcacaaa cagcgaaggt gtgaaaatgg gcaagactgt
     3841 aaaaggtgca gtctggcttg acaaggaaaa gacaccacca tatgagttgt atcagtattt
     3901 gcgcaatgtc gacgacgcag atgtcataaa atgtttgaaa atgctcacct tcgttccttt
     3961 ggaagaaata agggaaatgg aaaagcttga aggtagtgaa ataaatcagg cccaggttag
     4021 actagcttac gaagtcacca aaatggttca tggagaagag gaagcagaaa aggca
//
LOCUS       DAONVY010000171         4060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15591_length_4060_cov_3.674657, whole genome shotgun sequence.
ACCESSION   DAONVY010000171 DAONVY010000000
VERSION     DAONVY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15591_length_4060_cov_3.674657"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1366
                     /locus_tag="PLI11_05650"
     CDS             71..1366
                     /locus_tag="PLI11_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52381.1"
                     /translation="MKKYLPILISMLVLSSLSSCNKRKLKEVYDNTEETELLLGLWIP
                     PIYQLTPTQEEADKRYAEIKEAGMVMAYSIHVDPNNMDMLMRSLDAAEKNGIKMLVYL
                     MRYNNEKNLEIVEATKNHPAVLGYNLFDEPEYSEFDNIAQLRDEIKKIVPEDKIIMCN
                     MFPNYATDSVFGPNPNLYESNYNNYLDTYMQKVRPDVLSFDYYPFMKDPKADPDWIAG
                     MLTNLSDIRNIGKKYGVDTWGFVQNSGWSYTRVPNANELRFICHLHLIFGLKSYSYFL
                     YCQPNDKPGTAGIFEGMLTFHGEKTDIYYRVKKQNKDLKKMKGVFLNYDHVGFVTHNM
                     TKKHTDAIAKDLRYDKYKELEKIKSKGSILVGIFEKDGKTGLYVMNFDYKKNNKVTLE
                     LDSKTEFKVWGDDGLEQMKKADSIKLKLDPGEGKFIELG"
     gene            1436..2746
                     /locus_tag="PLI11_05655"
     CDS             1436..2746
                     /locus_tag="PLI11_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52382.1"
                     /translation="MKKYLCLFMSMLLLTSVTSCDSSKEDKIDLQEVYDNTEETELLL
                     GLWVTPVTHLTSTPEDAEARYAEIKEAGIVMAYTHHHETHDMEQLMRALDAAEKNGIK
                     MMVSLLPDNYRKSMNIVEATKDHPAVYGYNMADEPGGAAFDSLAKLRDDIKAIVSEDK
                     IIMCNMFPNYAPDWALGTKASDGNTVYYNYLDQYMEKVRPDILSFDHYPFMADSNADF
                     NRILIMLDNLSDVRNVGMKYNVDTWGFVQNSSWAGTRIPNDDELRFVCHLHLIFGLKS
                     YSYFLYCQPSDQSGVEGVFEGMLTYHGEKTDIYYRVQKQNQDLKKMKGVFLNYDHVGF
                     VTHNMDERHVNAIRENLRFGTYKELEKIDSEGMILTGLFEKDGKTGLYVMNFDYKQDN
                     TLTLKLNTKSDFKVWGAGGLEQMKKSNSIKLSLKPGEGKFIELG"
     gene            2765..3550
                     /locus_tag="PLI11_05660"
     CDS             2765..3550
                     /locus_tag="PLI11_05660"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HPZ52383.1"
                     /translation="MQMNSTLRVLAVGNSFSEDSVEYLYQIFKETGHDKDYDEIVIGN
                     LVVGGCSLDMHVNFALENKPVYLYTKFSKESNGDMEVVSTNATLLKGLQDEEWDIITL
                     QQRSGFSGDKDTFYPYLDMLINYINKHIIKKNAVLAWNLTWAYDEDSTHPDFARYNGS
                     QTEMYNSIVKAALDVIVPNKSISFVIPTGIAIQHARNKYKNLTRDGFHLSIPFGRYIA
                     ALTWFCTFTNRDIDVLETDILSKEQLETAKNSVKKATRNNLGR"
     gene            complement(3571..>4060)
                     /locus_tag="PLI11_05665"
     CDS             complement(3571..>4060)
                     /locus_tag="PLI11_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52384.1"
                     /translation="HSQYEIFIDMQTTFERDDQWMACFLGVRTQYNSGDQGIPTDPTS
                     GFWVAICKDRATIYPGTANQWAAGLVQIQLPESAETMHTYIIVDDGQCVYYYMVLSSG
                     ERHLLLKADISGETTVITDANGEELLQCDNMIQDNTGGYFRIFNHMGNTIVDKVVIKG
                     YR"
BASE COUNT         1378 a          808 c          828 g         1046 t
ORIGIN      
        1 caaatgcatt tacaataatc attggtaatg atatgcttaa aacagttaaa ccgacaacaa
       61 ggaggaaatc atgaaaaagt atttgcccat tttaatctca atgcttgtat tgtcatcact
      121 atcctcctgc aacaagcgaa aactgaagga ggtatatgac aataccgaag aaacggaact
      181 tcttcttgga ctgtggattc ccccaattta tcagctgact ccaacgcagg aagaagcgga
      241 caaaagatac gcagaaatca aggaagcagg catggttatg gcttattcca ttcatgttga
      301 tccaaataat atggatatgc ttatgaggtc actggatgcg gcagaaaaga acggtataaa
      361 aatgcttgtc tacctcatgc gctacaacaa cgaaaaaaat ctggagatag ttgaggcgac
      421 aaaaaaccac ccggcagtac tgggatacaa cctgtttgac gaacctgagt attccgagtt
      481 tgacaacatc gcacaactaa gggacgaaat aaaaaaaatt gtgcctgaag acaaaatcat
      541 catgtgcaac atgtttccca attacgccac cgattctgtt tttggcccaa atcccaattt
      601 gtatgaaagc aattacaaca attacctgga cacctacatg cagaaagtaa ggccggatgt
      661 attgtccttt gactactatc cttttatgaa ggatcccaaa gcagatcccg actggattgc
      721 tggcatgctt acaaacctaa gtgatattag gaatattggc aagaaatacg gcgtggatac
      781 atggggtttt gttcaaaaca gcggctggtc ttacacaaga gtaccgaatg caaacgagct
      841 cagatttatt tgccacctgc acctgatttt cggactaaaa agctactcgt actttcttta
      901 ctgccagcct aatgacaaac ccggaacagc aggcatcttt gaaggtatgc ttacctttca
      961 tggcgaaaaa accgacatct attacagagt taaaaaacag aacaaagacc ttaagaagat
     1021 gaagggagta tttttaaact acgatcatgt agggtttgtg acccacaata tgacaaaaaa
     1081 acacacagac gcaatcgcca aggaccttag atatgataaa tataaggaac tggaaaaaat
     1141 taaatcaaaa gggtccatcc tggtaggcat atttgaaaaa gacgggaaaa caggtcttta
     1201 tgtaatgaac ttcgactaca aaaaaaacaa caaggtgact ttggaacttg attcaaaaac
     1261 agagttcaaa gtttggggag atgatggtct tgaacaaatg aaaaaggcag actcaataaa
     1321 actcaaactg gacccaggcg aaggcaaatt catagaactt ggttgaaaca atatttacat
     1381 ccccaaatct tgatgataag atattatcac ttagttaaac cacaaggagg agaccatgaa
     1441 aaagtattta tgcttgttta tgtcaatgct tctactgacg tcagttactt cctgtgacag
     1501 cagtaaagaa gacaagatcg accttcaaga agtgtacgac aacactgaag aaacggaact
     1561 attgttggga ctgtgggtaa ctccggtaac ccacctgaca tccactcccg aggatgctga
     1621 agccagatat gcggaaatta aagaagcagg tattgtaatg gcatacaccc atcatcatga
     1681 aacccatgat atggagcagc ttatgagagc actggatgcc gctgagaaaa acggcattaa
     1741 aatgatggtt tcgctattac ccgacaacta taggaaaagc atgaacattg tcgaggcaac
     1801 aaaggaccat cccgcagttt acggctacaa catggctgat gagcctggag gagccgcctt
     1861 tgatagtctt gcaaagttga gggatgatat caaggcaatt gtgtcagaag acaaaatcat
     1921 aatgtgcaac atgtttccaa actatgctcc cgactgggct cttggcacaa aggcatccga
     1981 tggaaacacc gtttactaca actacctgga tcaatacatg gaaaaggtaa gacccgacat
     2041 tctgtctttt gaccactacc ctttcatggc cgacagtaac gcggacttca acagaatact
     2101 cataatgctg gacaatctga gcgatgtcag aaacgtaggc atgaaatata atgtggacac
     2161 ctggggattt gttcagaaca gcagctgggc aggcactaga attccaaatg atgatgaact
     2221 tagattcgta tgccatctcc acctcatatt cggactaaag agctattcat attttctgta
     2281 ctgtcagcct tccgaccagt ctggagtcga aggagtattc gaaggaatgc tgacatacca
     2341 cggcgaaaag accgacatat attacagagt tcagaagcag aatcaggacc ttaagaaaat
     2401 gaaaggcgtc ttcctgaatt acgaccatgt aggatttgta acccacaaca tggacgaaag
     2461 acatgtcaat gcaataaggg agaatttgag atttggtaca tacaaggaac ttgagaaaat
     2521 cgactctgag ggcatgattc ttacaggtct tttcgaaaaa gacggaaaga ctggtcttta
     2581 tgtcatgaac tttgattaca aacaggacaa tactctgact ctgaaactaa acaccaaatc
     2641 agatttcaag gtttggggtg caggaggtct ggaacaaatg aagaagtcaa attcaataaa
     2701 acttagcttg aaacccggtg aaggcaaatt tattgaactt ggataattga aaggattatt
     2761 gaacatgcag atgaacagta cacttagagt tcttgcagtt ggaaacagtt tcagcgaaga
     2821 cagcgtggaa tatctttatc aaatctttaa agaaacaggt catgataaag actacgatga
     2881 aatcgttata ggaaaccttg ttgtgggagg ctgcagtcta gatatgcatg tgaattttgc
     2941 tttggaaaac aagccagttt atctatatac aaagttttct aaagaaagca atggagacat
     3001 ggaagttgta tcaacaaacg caactcttct taaaggcttg caggatgagg aatgggacat
     3061 aatcaccctc cagcaaagaa gcgggtttag cggagacaag gatacctttt atccctatct
     3121 cgatatgtta ataaactaca ttaataaaca cattataaaa aagaatgcag ttcttgcgtg
     3181 gaatcttacc tgggcatatg atgaagactc tacgcatccg gactttgcaa gatacaacgg
     3241 ctctcagaca gaaatgtata attcgatagt gaaagctgca ctagatgtaa ttgtacctaa
     3301 taaaagcatt agctttgtaa tcccaacagg tattgcaatc caacatgcaa gaaataagta
     3361 taaaaaccta acgagagacg gatttcacct ctcgatacct tttggcagat atattgcagc
     3421 actgacctgg ttctgtacat ttacaaacag ggatatagat gttctggaaa ccgacatact
     3481 atcaaaagaa caacttgaaa cagcaaagaa ttctgttaaa aaagcaacaa gaaataattt
     3541 agggagatga tttttcatct ccctgattta ttacctatac ccttttataa ctaccttgtc
     3601 aactattgtg ttacccatgt ggttgaatat tctaaagtat ccaccagtat tatcctgaat
     3661 catgttgtcg cattggagta gttcttctcc atttgcatca gtaataactg ttgtttctcc
     3721 ggatatatcg gctttcaaca gcaaatgtct ttcgccactt gacaatacca tatagtaata
     3781 cacacattgg ccgtcatcaa ctataatata tgtatgcatg gtttctgcac tttctggcaa
     3841 ctggatttga acaagtccgg cagcccattg atttgctgtt cctggataaa tagttgccct
     3901 gtccttgcaa attgcaaccc agaatcctga agtcggatct gttggtatac cctggtcgcc
     3961 gctgttgtac tgcgttctta ctcccaggaa acatgccatc cactgatcat ctctttcaaa
     4021 cgtagtctgc atgtcaataa aaatctcata ctggctgtgg
//
LOCUS       DAONVY010000172         4058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15600_length_4058_cov_3.837372, whole genome shotgun sequence.
ACCESSION   DAONVY010000172 DAONVY010000000
VERSION     DAONVY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15600_length_4058_cov_3.837372"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLI11_05670"
     CDS             <1..310
                     /locus_tag="PLI11_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52385.1"
                     /translation="EVFDTRWSHCHQWSGCPTWILTHYGLGLAKRFTKGVNHFEFKLR
                     PGRIKSAKGAIPLPCGSKVYTEWKDGIYKVLPEKDIVIHFDNGETLECKANTETVFDI
                     "
     gene            complement(366..1550)
                     /locus_tag="PLI11_05675"
     CDS             complement(366..1550)
                     /locus_tag="PLI11_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPZ52386.1"
                     /translation="MKPNYRRRAKLLKLFTFFILLAAILYITYIGPLLKFINNYIPDD
                     STDGDPVLSPGAPVVDLSGYLNPTPTPVPTISETPDNGSTPDPIITSSPTPTPVPTPT
                     IPYVPDPKVPGVKHINGKDLRLDSDSINLLMLGVDEKYSNVDSIFIVSISNSKKSIKV
                     ISVPRDAYVPYPENIASLLKSPRYYFKGIFKVNAAYHVGNNILNYQDGIFASKGINFL
                     CSVLDGLLVNVNLTIHDYVRVNFEGFTEIVDIAGGVYVDVPEDMYNSNGKLFLAKGRQ
                     FLNSKQALEFVRRRNRYDSNGKMISSSGDPYRKANQLAFLKGCAEQLVTVDSITKAPQ
                     ILESLSKNVHHSVNNFSKMSKYMGIAMDYASGKYEVELLLIRGKSIDPLGDRASYVDI
                     IT"
     gene            complement(1601..2443)
                     /locus_tag="PLI11_05680"
     CDS             complement(1601..2443)
                     /locus_tag="PLI11_05680"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPZ52387.1"
                     /translation="MLKIGCHLSNAKGYVNMMNQALDIGANTFQFFTCNPRGSRVKNI
                     DHEDIGNFNNMMKENNFNKIVGHAPYTLNPASPDNRVLNFAYLTVEEDLSLMELMPGN
                     FYNMHPGSHVKSGVEEGIKRVIDVLNQNMSNTMTTTILLETMSGKGSEIGRTFEELLM
                     IIDGLNHPDKVGICLDTCHVYDGGYDIKEDLDGVLYELDKVIGIERLHAIHINDTKNP
                     FASHKDRHEKIGDGYLGEEFFFKLINHKDLKDKIFILETPNEIEGYAAEIKLLKYNYG
                     NIDF"
     gene            2645..>4058
                     /locus_tag="PLI11_05685"
     CDS             2645..>4058
                     /locus_tag="PLI11_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPZ52388.1"
                     /translation="MRHELRKIGKNEYEVLIFLDDDAEFAMEPGEKATYNPRSIREFI
                     KSKYPNLRITAAKVMIGGLLIASIPIGASLAKPVPVAAATCIVQQDSSIYYRVKQGNS
                     LWGIAQKFNTTVNAIKDSNNLTANTIYVNQPLVIPKAVHTVKKGEYLSLIAKNYNTTV
                     SALKEANNLTGDLIIVGQRLVIPTHVDASRYTVRPGDSLWSIAQKYGVTVNSLKEANG
                     LTNNVIHTGQELIIPEENHDNLGYTVKAGDTLYAVARKYNVSVESLKKANNLSTNTIY
                     VGQKLVIPTDDQTNDAGTEATVKLQQNLKKLGYLDVPAYSGNYDEPTVTAIKKFQANY
                     QLNVTGKADSATITAIEHAVVKQGIVVDTRNYIGVPYLWGGSTPNGFDCSGFVYYMFN
                     KHGVKMPRQSSASLFSQGTSVSKSNLRLGDLVFFAVNSTGTISHVGFYLGDNEWISAT
                     TSKGIAVYSMDNTYWSQYYVG"
BASE COUNT         1224 a          822 c          820 g         1192 t
ORIGIN      
        1 tgaagtgttt gataccagat ggagtcactg tcatcagtgg tcgggatgcc caacttggat
       61 tttgacacat tatggtcttg gattggccaa gcgcttcaca aagggggtaa atcactttga
      121 gtttaagtta aggccaggaa gaattaagag tgccaaggga gcgatacctc tcccttgtgg
      181 cagcaaagta tatactgaat ggaaagatgg tatatacaaa gtactccccg aaaaagacat
      241 tgtcatccat tttgacaacg gagaaacact ggaatgcaag gcaaacactg aaactgtatt
      301 tgatatataa aggcatcaaa aaaaccagga gcttatttaa gttcctggtt tccgtttact
      361 tcggattaag taattatatc tacatagctt gccctgtcgc ctaaggggtc tatcgatttg
      421 cctcgtataa gcagcaattc cacttcatac tttccgcttg cataatccat agcaatcccc
      481 atatactttg acatctttga gaagttattc acactatgat gcacgttttt gcttagagac
      541 tcgagtattt gcggtgcttt tgttatacta tccacagtaa ccagctgttc cgcacagccc
      601 ttcaggaaag cgagctggtt tgcctttcta taggggtcgc ctgagcttga gatcatcttg
      661 ccgttggaat catatctatt tcttctccga acaaactcaa gagcttgttt tgaattcaaa
      721 aactgtctac cttttgccaa aaacagcttg ccgttagaat tgtacatatc ttctggcacg
      781 tcaacataaa ctccgcctgc tatatccaca atctcggtaa agccttcaaa attgactctt
      841 acataatcat gaatggtaag gttgacattt accaacagtc catccagaac gctgcataaa
      901 aagtttatgc ccttgcttgc aaatatcccg tcctggtaat ttagaatatt gtttccaacg
      961 tggtaagcag cgtttacctt gaaaataccc ttgaaataat atctggggct ctttaaaagg
     1021 ctggcgatgt tttcagggta gggcacataa gcatcccttg gaacagaaat aactttaatg
     1081 ctcttcttgc tgttactgat gcttacaata aatatggagt ccacgttgct gtatttctca
     1141 tcaacaccca acatgagcaa atttatactg tcagaatcca gcctcagatc cttgccgttt
     1201 atatgcttta ctcctggtac ttttggatca ggtacatagg gtatggttgg agtaggaacc
     1261 ggagttggcg taggggatga agtaataatc gggtccggag ttgaaccgtt atcaggtgtt
     1321 tctgaaatcg taggtacggg tgtcggagtt ggatttagat atccgctcaa atcaacaaca
     1381 ggcgcacctg gacttaatac agggtctccg tctgttgaat cgtctgggat atagttgtta
     1441 ataaatttca ataaaggccc aatatatgta atatataata ttgcggctaa caaaataaaa
     1501 aatgtaaata actttaaaag tttggctctt cttctgtaat taggtttcat tcagaatcct
     1561 ccccatcaaa aatttcatgg ttatagtatc accgagatac ctaaaagtca atattaccgt
     1621 aattgtactt aagcagcttt atttctgctg catatccttc aatctcattc ggggtttcaa
     1681 gaataaatat tttgtccttc aaatctttat gattaatcaa tttgaaaaag aattcttctc
     1741 ccagataacc atcgccaatc ttttcgtgtc tgtccttgtg gctggcaaag gggttcttgg
     1801 tatcgtttat gtgaattgca tgcagccgtt caatcccgat aaccttgtca agctcataaa
     1861 gtactccatc caaatcttct ttaatgtcgt atcctccgtc gtatacatgg caagtatcaa
     1921 ggcatatgcc aaccttgtca ggatggttaa ggccatcgat tatcatcagc agctcttcaa
     1981 aagtcctgcc gatttcagac ccttttccag acattgtttc aagcagtatg gtagtagtca
     2041 tggtgtttga catgttttgg ttaagaacgt caattactct tttaatgcct tcttccactc
     2101 ctgatttgac atgacttcca ggatgcatgt tgtagaagtt gcctggcatt aattccataa
     2161 gggacagatc ttcttccaca gtgagataag caaagttcaa cactctattg tcgggagaag
     2221 caggattcaa tgtgtatggg gcatgcccga caatcttgtt gaaattgttc tccttcatca
     2281 tgttgttaaa attgccgata tcttcatggt ctatgttttt tactctgctg cctcttggat
     2341 tgcaggtaaa gaactgaaaa gtattagctc ctatgtctag agcctggttc atcatgttta
     2401 cataaccttt tgcattggac aagtgacaac caattttcaa catattgttt actcctcctt
     2461 tctgactatt atacatcttt ccagcaaaat gtttaagcaa caaacagaaa acatacgata
     2521 aataatagtt aaacaatgaa gaaacaaagt cgcacgagat gcatctcttc aactttttta
     2581 tttcctttaa tgtattgatt caattgttca tgcagataat aaataaaaag aagggagtat
     2641 caatatgcga cacgaattaa ggaaaattgg taaaaacgaa tatgaagtcc tcatatttct
     2701 cgatgatgat gcagagttcg ctatggaacc aggagagaaa gcgacctaca atcccaggag
     2761 cataagggaa tttataaaga gcaagtatcc caacctgaga ataactgctg cgaaggtcat
     2821 gatcggcggg ctattgatag cttctatccc aataggtgca tcgcttgcaa agcctgtgcc
     2881 tgtagctgct gctacctgca ttgtgcagca ggacagttca atatactacc gggtgaagca
     2941 gggtaattca ttatggggta ttgcgcaaaa attcaacaca acggtaaatg caatcaagga
     3001 ctcaaacaac ttgacagcaa acactattta tgttaatcag cctttggtta tcccgaaagc
     3061 tgtgcatact gttaaaaagg gagaatacct ttccttgatt gcgaaaaact ataatacaac
     3121 cgtatcggcg ttaaaagagg caaacaacct tacaggagat ctaataatag tgggacagag
     3181 acttgttatt cccacacatg tggatgcttc caggtacacc gtaagacctg gcgattcctt
     3241 gtggtcgata gctcagaaat atggtgtaac tgtcaacagt ttaaaagaag caaacggtct
     3301 gaccaacaat gtcatccata caggacagga actcataatt ccggaggaaa accatgataa
     3361 ccttggctat acggttaaag caggagatac attatatgca gtagcccgaa agtacaatgt
     3421 aagtgtcgag agtttgaaaa aagcaaacaa tttaagtacg aacaccattt atgtgggcca
     3481 aaaacttgtg atacctacgg atgatcagac aaacgatgca ggcacggaag ccactgtaaa
     3541 actacagcaa aatcttaaaa agcttggtta ccttgatgtt cctgcttact ccgggaacta
     3601 cgatgagcct acagtcacgg caataaagaa atttcaagcg aattatcaat tgaacgtcac
     3661 aggtaaggcg gacagcgcaa ccattacagc tatcgagcat gcggtagtta aacagggcat
     3721 tgtagtagat acaagaaatt atataggtgt gccttatctc tggggaggaa gcacacccaa
     3781 tggttttgat tgctcaggct ttgtttatta catgttcaac aaacatggcg taaaaatgcc
     3841 aagacagtct tcggccagtt tgttttccca gggcacaagt gtttcaaaga gcaatctgcg
     3901 tctaggtgac cttgttttct ttgcggttaa ctccacaggt accatttcac atgtcggttt
     3961 ctatctggga gataatgagt ggatatccgc aaccacttcg aaaggtattg cagtttactc
     4021 aatggataat acctattggt cacagtacta cgtaggag
//
LOCUS       DAONVY010000173         4049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15640_length_4049_cov_3.867802, whole genome shotgun sequence.
ACCESSION   DAONVY010000173 DAONVY010000000
VERSION     DAONVY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15640_length_4049_cov_3.867802"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLI11_05690"
     CDS             complement(<1..896)
                     /locus_tag="PLI11_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase family polysaccharide lyase"
                     /protein_id="HPZ52389.1"
                     /translation="MIEDLMVIKQRYYEYLWRNVEDAEEYLPLLENEGDLYRWSDLNC
                     QDSNTANWQGHTFLNRTRLVLQRYGRDMDKELFDKLAGAFRYWLKHDFQNTNWWYMQI
                     GVPSALCDLTIMLEDELTEEELDKAVSIIQRGSFSHNQHAYNMTGANLIWGVWTTVKH
                     ALLVQDCELLKKAVDRASYEFEIADGLKEGVKPDYSFFQHGPQHYTMGYGRSFTESLS
                     PMVYILSGTEYQFSKEILKLLVDFLLEGQVYMIRNGTVDYLAIGREYCRPEAYTSIAI
                     RRAAELYSKTSSIPRKADLEAF"
     gene            complement(1142..1807)
                     /locus_tag="PLI11_05695"
     CDS             complement(1142..1807)
                     /locus_tag="PLI11_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52390.1"
                     /translation="METGKKAINIITGRYGMGTSELNHIVDAIFGGIHARERFETYFH
                     WYNLLHELGHGIMCFNADVRPHPISEEQIVNDFAVAYWLIYGENTKINFLDKIISNAL
                     ENITCPAPSGVSHIEYAYEKWNTEDFHNFNNYGWFQFSCVKDSLRKRKNLEIVLTQMG
                     VKNIKVQPKKTFIYPVIDENVVREIIDDAVSVLRKWGVKLPDTYVTFDSDPNKHMCNV
                     IDL"
     gene            complement(1901..3523)
                     /locus_tag="PLI11_05700"
     CDS             complement(1901..3523)
                     /locus_tag="PLI11_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPZ52391.1"
                     /translation="MWRHLFKEHILDKGIDYFITNRVGNVYTKDNMIEATVYGTEEYK
                     VGIVKDDEEIIDLSCNCPYADAGYNCKHMAAVLFYLEEKNAFLSQDIEESLVKLVEEA
                     EPTLIKEFLIDVLRTDEKLLNRFKSRLQCVDMECYKNQINNIFMRYSGYHGFVDYKNA
                     WGFISELEEILDNDIQAMLDNDQLKEAFDLTNYIFVKVANQDMDDSDGGTGEIAGRCL
                     EIWQEILDNCSIDFKRDMYKWFVEHLDGSVIDYMEDYVEKILFEDFREEEFMESKLMY
                     LDDKIKEYKVEKDSWFNQYKLGKLILRSIETLEEMNISQQEIDEYCRENIEIDEVRKY
                     YIDICINKKDYDIAITLLKEGKDKGKDLPGLLIYYSMKLKDLYKEIGEKQLYEEELWS
                     LVLDYNAGDVELYKELKSIYTEEEWLEKREIIFSKLSPYSGIDKLYEMEGLYDRLIDL
                     VMSSYGLYMLMEYEEILKDIYPKELLKKYEYTVKDMATKTTSRTRYREIVSILRRMEK
                     YPGGKELVLEITNDWKSRYKNRTAMMDELSKL"
BASE COUNT         1224 a          901 c          497 g         1427 t
ORIGIN      
        1 ataaatgctt caagatcggc ttttcgcgga atagatgaag tttttgaata cagttccgca
       61 gctcgtctaa ttgctatact ggtatatgcc tccggcctgc agtattcacg acctatggca
      121 agatagtcaa cagtaccgtt tctaatcatg tacacttgac cttcaagcaa aaagtcaaca
      181 agcagcttca gtatttcttt agagaactga tattcagttc ctgatagtat gtataccatt
      241 ggtgataggg attctgtaaa cgatctgcca taccccattg tataatgctg ggggccatgc
      301 tggaagaagc tgtagtcagg ctttactcct tcctttaagc catctgcaat ttcaaactca
      361 taagaggcac gatccaccgc ctttttcaaa agctcacagt cctgaacaag gagggcatgc
      421 tttactgtag tccatacacc ccatatcaga ttggcacctg tcatgttata tgcatgctga
      481 ttgtgggaaa aagaacctct ttgaataatt gaaacagctt tgtcaagctc ctcttctgtt
      541 aactcatcct ccagcattat tgtcaggtca cacaaagcag aaggcacacc tatttgcata
      601 taccaccagt tggtgttttg aaaatcatgc tttaaccagt agcggaaagc gccagcaagt
      661 ttatcaaaaa gctccttgtc catatctctt ccgtaccgct gcagtacaag gcgtgttcta
      721 ttcaagaatg tatgtccctg ccagttagcc gtgtttgaat cttgacaatt caagtctgac
      781 catctgtaca aatcaccctc gttttccagt aaaggcagat actcctccgc atcttccaca
      841 ttacgccaca gatattcata gtacctctgt tttataacca ttaaatcctc tatcaaaaca
      901 gtatcctcct tttacccttg tcttgatttt agattaaagt atcccgcata caagcacttt
      961 gtctactaca aactcctcaa agctatcaat atctttagta gagcttatcc ttgccgtctg
     1021 cctcaattaa tacagcacag tcaggattgt agctgccagc ttggcagtta tgcatacaat
     1081 ttgactcaaa gctttttgca aaactcatca ataaaatgca tcctatgcct tctaaatcat
     1141 tctataaatc aataacatta cacatatgct tattggggtc agaatcaaaa gtaacatatg
     1201 tatcaggtag ttttactccc cattttcgta atacagacac agcatcatca ataatttctc
     1261 ttactacatt ctcatcaatc accggatata taaaagtttt cttcggctgt acttttatat
     1321 ttttgactcc catctgagtc aatactattt ctagattttt tcttttacgt agagagtctt
     1381 ttacacaaga aaattgaaac caaccataat tattgaagtt atgaaaatct tctgtgttcc
     1441 atttctcata tgcatactca atatgagata ctcctgatgg agcagggcat gttatgttct
     1501 ccaaagcatt tgatataatt ttatccagaa aatttatctt agtattttct ccataaataa
     1561 gccaatatgc tacagcaaaa tcatttacta tctgttcctc acttataggg tgtggacgca
     1621 catctgcgtt aaagcacatg ataccatgtc ccaactcgtg caataaatta taccagtgaa
     1681 agtatgtttc gaatctttct ctcgcatgta ttcctccaaa aatagcatca acaatatgat
     1741 ttaattctga tgtacccata ccatacctac ccgttattat gttaattgcc tttttacccg
     1801 tttccatatg ttatctccta ttcataaata caaattttac taattacccc acaaatcaga
     1861 ctatgcattt tttaattttg agctcagatt atagccacct ttacaattta cttaattcat
     1921 ccatcattgc agttctattt ttatatcttg atttccagtc attagtaatt tctaacacta
     1981 gctctttacc tcctggatat ttctccattc ttcttaaaat cgatactatc tctctatagc
     2041 gagttctact tgttgttttt gttgccatat cctttactgt atactcgtat tttttcaaaa
     2101 gttcttttgg ataaatatct tttaaaattt cttcatattc cataagcata tataatccat
     2161 aagagctcat aaccaaatca attaatctat catacaatcc ctccatctcg tataatttat
     2221 caattccact atatggagat aacttactaa aaattatttc tctcttctct agccattctt
     2281 cttccgtgta tattgatttt aactctttat ataattccac atctcctgca ttataatcta
     2341 atactaatga ccacagctct tcttcataaa gttgtttttc tcctatttct ttatataaat
     2401 cttttagctt catgctataa tagattaaaa gccctggcaa atcttttcct ttgtctttac
     2461 cttccttaag caaggtaatt gctatatcgt agtccttctt gttaatacaa atatcaatat
     2521 aatattttct aacttcatca atttctatat tttctctaca atactcatca atctcttgtt
     2581 gacttatatt catttcctct agagtctcta tacttcgtaa aattaattta ccaagtttat
     2641 attgattaaa ccatgaatct ttttctactt tatattcttt tatcttgtca tccaggtaca
     2701 tgagtttgct ttccatgaac tcttcttctc taaaatcttc aaataaaatc ttctctacat
     2761 aatcctccat ataatctata acagacccat ccaaatgttc aacaaaccac ttgtacatat
     2821 ctcttttaaa atcaatactg caattgtcca atatctcttg ccatatctct aaacatctgc
     2881 cagctatttc tcccgttccg ccatccgaat catccatatc ttgatttgct actttaacaa
     2941 atatgtaatt cgtaaggtca aaggcctctt ttaattgatc attatctagc attgcttgta
     3001 tatcattgtc tagaatttct tcaagttctg atatgaatcc ccaggcattt ttataatcaa
     3061 caaaaccatg ataccctgaa tatctcataa atatattatt tatttgattt ttataacatt
     3121 ccatatcaac acattgcaat ctacttttaa atctatttaa taatttttca tcggttctta
     3181 aaacatcaat taaaaactct ttaattaaag taggttctgc ttcttccacc aatttaacaa
     3241 gactctcctc tatatcctga cttaggaaag catttttttc ttccaaataa aataatactg
     3301 cagccatatg tttgcaattg tatccagcat ccgcatatgg acaattacaa cttaaatcta
     3361 tgatttcctc atcatccttc acaatgccaa ccttgtattc ttccgttcca tacactgtag
     3421 cttctatcat attatcttta gtatatacat ttccaacacg gttagtaata aaataatcta
     3481 tacctttatc taatatatgt tctttaaata aatgcctcca cattttatca actccttaat
     3541 actaaggtgt atccacattt aattagtaca attatatgaa ccagtgagtc cgaatcaatt
     3601 gaccacttac aacttcgttt tgacactcat gtgctatata taataaaata caaaattgca
     3661 tattttaatg atagcatttt taattttgat aaacaagatt gtcaatctct ctggtgagca
     3721 ctacatacta ttttctgtat ttcctcatac tctcctcttc cagttcaatt ttgcatgtac
     3781 catgattaaa ctgcccaaaa cgccatcagc atcagcttgc ggctcacact cagctttccg
     3841 cattacatgc aaacttgtgc ggaacgaaaa aatcgcatgc accaaaagta ctcctcgcaa
     3901 cacttatgca aggggcgcat gcttactgat attgagttat ctataaagca aatgggataa
     3961 cggtgttttt ataaacatat cggtaacacc acggatagca gcagtactat agagaacaca
     4021 agcactgccc acccaagcca aacagagag
//
LOCUS       DAONVY010000174         4020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15773_length_4020_cov_2.879950, whole genome shotgun sequence.
ACCESSION   DAONVY010000174 DAONVY010000000
VERSION     DAONVY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15773_length_4020_cov_2.879950"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1491
                     /locus_tag="PLI11_05705"
     CDS             196..1491
                     /locus_tag="PLI11_05705"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPZ52392.1"
                     /translation="MKRLTAIILVVLTILSITSCAREGWKMTSPSKTIEDNSITYIYD
                     KFTGKKIENIYLEHGQRPEIMFNVESISGSLDVFIQDEYGKTVYENYDIQSGEFLVEL
                     SRAGDYKVILNAEDHVGSYNMTWSLPKASSDEYVNPYENLAGGKWLKANFHTHAGTGP
                     GTCGVNPQDVVIETYKQLGYELLTISNHDIFTDTSDFTDDKILMIPGVEYSPSSKGHM
                     LTIGITQSLHEHTNQKAIEMTNEMGGFVVLAHPNWGRQGYWPLNELLSLKGYIGIEVI
                     NQLIYRLSGSGLATNAWDFLLSRGKLVYGFGNDDLHALGDAGRSFNIIYCKERTWESM
                     KEAIDNGQFVASTGLYPEYLVLDGDTIRVKASYPIDTYVDTFTYRFIENGKVLSEQTA
                     REGVYKLNGEKYVRVEVIGENGAMLFFQPVYLKDTLVKP"
     gene            1729..>4020
                     /locus_tag="PLI11_05710"
     CDS             1729..>4020
                     /locus_tag="PLI11_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52393.1"
                     /translation="MKKRRILISVIIVAVLAAVTSLALYKFLDTNKNKSPGIITATEA
                     EIMDIYLDMLIEHKKFADTIFTDTSNIMEGTGYFGKGGGQKIRTHNNYTLYYAFLYTQ
                     LHGTKYMPEEDQENTEYAIKDKEAFLEEVKQKALAGIRYGIYTHNSVKKMQTIEAMAT
                     DSGKWWGPNHWQSSLYANGVLFAASLMWDHLDEPLKADVETVAAAEGDRAIRTNPRSY
                     APGNTGAEENAWDTNGPSLAYCMFPKHKNAEKWREAAIKFAMNTLSRELDRNDSAVID
                     GKPASKWITTSNLFSDYSLENHQILHPKYIASPLISFGDSVVAFAYHGKPIPEAFKYN
                     VQKVSDNVLKKLVYPTGEWIYPNGCDWTMILPGKLEAFALMSCLLGDEEALMLEARAA
                     KLAYERQKLTEDGRFVHIADIGTEREAVNVKRVVYSYLLHKHVGFKGDGSTATWESFI
                     DNHKGTTTFLDGFVITNNSISRFAALSWKNKLIGLVTPSSDKYSDNKYITHPNLPNIV
                     GSMSIVGTSADKAYITHKNYTVGEKFANGLYAGFSSIGKLSENGGAVSRYIAMTALPS
                     NAVIVMDSTVAERNLQIAAGYVVPISFQTDIVSGQKKTIITEKDKDSFTAGPGVNKEY
                     EGSYVLVDNHTSVILDKKMRITFGDYTLSSGLGGSTLKFYIEPGQFQKGQSINKTSAV
                     IFSNVEESELKKLKSDVEYLNMPEGWQGIFVKDTDGKKYYVVNNFYGQKGAFEYTGET
                     YAPIFMDDMTVAGKTATVSLNDGM"
BASE COUNT         1315 a          729 c          880 g         1096 t
ORIGIN      
        1 taggcatgga agcctttttt attgttgctt ccgtatcttt cttttcacag catctaattc
       61 ttaacaaagc taattttgta ttgcggatat ttaagtacaa ttattttaca tataatccaa
      121 tgaaaattac atatttataa caaatatttt aatatacaat aattttacga tacgtgaaag
      181 gaagaactaa ccaatatgaa aagattgaca gccattatac ttgttgtttt aaccatatta
      241 tcaattacat catgtgcaag agaaggctgg aaaatgacta gcccaagcaa aacaattgaa
      301 gataattcaa ttacttacat atatgacaag tttacaggca agaaaataga gaatatttat
      361 ctggaacacg gtcagagacc tgaaattatg tttaatgttg agtccatttc tggttccttg
      421 gacgtgttta ttcaagatga atatggtaaa acagtttatg agaattatga tattcaaagc
      481 ggagagtttt tggtggaact aagccgtgca ggcgattaca aggttattct caatgcagag
      541 gaccatgtag gcagttacaa tatgacgtgg agccttccaa aagcgtcttc cgatgaatat
      601 gtaaatccgt atgagaattt ggcaggaggc aaatggctca aggccaactt ccatactcat
      661 gccggaaccg gtccgggcac ctgcggtgtg aatcctcagg atgtggttat cgagacatac
      721 aagcaacttg gttacgagct gttaaccata tccaaccacg acatatttac agacacatcg
      781 gattttacag atgacaagat attgatgatc cccggtgttg agtattctcc atctagcaag
      841 ggtcacatgc tgaccattgg aattactcaa tcccttcatg aacatacaaa tcaaaaagct
      901 atcgaaatga cgaacgaaat gggcggtttt gtagtattgg cacatccgaa ctggggaagg
      961 cagggttact ggcctcttaa tgaactgctt tcactgaagg gatatattgg tattgaggtt
     1021 atcaaccagc ttatataccg tctatcagga tcaggccttg ccacaaacgc ttgggatttc
     1081 cttctttcaa gaggaaagct cgtttatggt tttggaaatg acgatctgca tgcacttggc
     1141 gatgctggca gaagctttaa tataatctac tgcaaggaaa ggacctggga gtccatgaag
     1201 gaagcgattg acaatggaca gtttgtagca tcaacaggct tatatcctga gtacttggta
     1261 cttgacggag acactattag agttaaggca agttatccta ttgataccta tgttgatacc
     1321 tttacataca gatttattga gaatggaaaa gttttgtccg agcagacggc aagagaaggt
     1381 gtgtataagc tgaatggcga aaaatacgta agggtagagg ttattggaga aaatggagcg
     1441 atgctctttt tccaaccggt atacctgaag gatacacttg ttaaaccata atgatgtgaa
     1501 ttaataaact tttatacaag gtcttcatat ggaagattaa attgtttcgg ttggctttaa
     1561 tcttactagt ggaccgatgt atttattata tagaataact taaaaagact tcatatcaaa
     1621 gttttaattc tttttttatt aattatgata aaatgttatt agagataaca tctgcctttt
     1681 ttagaaaaaa ttgaataggt tgaacctaac caattaaggg gagataatat gaagaaacgt
     1741 cgaattctga taagtgttat tatagttgca gtcctggctg ctgttacttc cttggctcta
     1801 tataaattct tagacacaaa caagaacaaa tctccaggca taatcacagc tactgaagcg
     1861 gagataatgg atatttatct cgatatgctt atcgagcaca agaagtttgc agacaccatc
     1921 tttactgata caagcaatat catggaaggc acaggctatt ttggcaaggg tggaggtcag
     1981 aagattagaa cgcacaacaa ctacaccttg tactacgcct tcctgtatac acagcttcat
     2041 ggtaccaaat acatgccaga agaagaccag gaaaataccg aatatgctat aaaagacaag
     2101 gaagccttcc ttgaggaagt aaaacaaaag gctttggctg gtattcgtta tggaatatat
     2161 actcacaact ctgtaaagaa gatgcagaca atagaggcaa tggcaaccga ctcaggcaag
     2221 tggtggggtc ccaatcactg gcagtcatcc ctctatgcca atggagtttt gtttgctgct
     2281 tcgctgatgt gggatcatct tgatgaaccg ctgaaagcag atgttgagac ggttgcagct
     2341 gctgaaggag acagggcgat aaggacgaat ccgagaagct atgctccagg aaacacagga
     2401 gctgaagaga atgcctggga taccaacgga ccatcccttg catactgcat gtttcctaaa
     2461 cacaagaatg cggagaaatg gagagaagca gcaatcaagt ttgcgatgaa caccttgtct
     2521 cgtgaacttg acaggaatga tagtgctgta atagatggaa agcctgcatc caaatggata
     2581 acaacatcta atctttttag tgattattct cttgaaaacc accaaattct tcatcccaaa
     2641 tacatagcaa gtcctcttat cagttttgga gattccgttg tggcttttgc ttatcacgga
     2701 aaaccaatac cagaagcatt caaatacaat gtccagaaag taagcgataa tgttttgaaa
     2761 aagcttgtat acccgaccgg agaatggatt tatcccaacg gatgcgactg gaccatgatt
     2821 ctgcctggaa aactcgaggc gttcgccttg atgtcctgtc tcctaggtga tgaagaagct
     2881 ttgatgcttg aagccagagc tgcaaagctt gcatatgaaa gacaaaaact cacagaggat
     2941 ggcagattcg ttcatattgc agatattggc acggaaaggg aagctgtaaa tgtaaagagg
     3001 gtggtctatt catatctttt gcacaagcat gttgggttta aaggtgatgg ttctacagca
     3061 acctgggaaa gttttataga caaccacaaa ggcacaacca ccttcctgga tggatttgta
     3121 ataaccaaca acagcataag cagatttgca gcgctttcat ggaagaacaa acttattggc
     3181 ttggttacac caagcagcga caagtattcg gacaacaaat acataacaca tccaaacctg
     3241 ccgaatattg tcggatcaat gtccattgta ggcacatcag cagacaaggc atatataacc
     3301 cataaaaatt acacggtggg agagaaattt gcaaacggac tatatgctgg attctcgtcc
     3361 ataggcaaat tgagtgaaaa cggtggagcg gtatcaagat acatagcaat gactgctctt
     3421 ccgtctaatg ctgtgattgt catggacagt accgttgcag aaaggaatct ccagatagcc
     3481 gcaggttatg tggtacccat atccttccag acagatattg tttcaggaca gaaaaagaca
     3541 atcattactg agaaggataa agacagcttt accgcaggtc ctggagttaa caaagagtat
     3601 gaaggatcat atgtgttggt tgacaatcat acatcagtga tactggacaa aaagatgagg
     3661 atcacctttg gcgattatac attgagctct ggtttgggtg gctccacatt gaagttctat
     3721 atcgagccag gacagtttca gaaaggacag agtataaata aaacatcagc tgtcatattc
     3781 tccaatgtgg aagaatctga gcttaagaaa cttaagagtg atgttgaata tctcaacatg
     3841 cctgaaggat ggcagggcat attcgtaaaa gatactgatg ggaaaaagta ctatgttgta
     3901 aacaactttt atggtcagaa gggtgcattt gaatacactg gagaaaccta cgcaccaata
     3961 ttcatggatg acatgactgt agctggcaaa acagcaacgg tttcccttaa tgatgggatg
//
LOCUS       DAONVY010000175         3990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15915_length_3990_cov_4.280305, whole genome shotgun sequence.
ACCESSION   DAONVY010000175 DAONVY010000000
VERSION     DAONVY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15915_length_3990_cov_4.280305"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLI11_05715"
     CDS             complement(<1..832)
                     /locus_tag="PLI11_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52394.1"
                     /translation="MRKLLFFVIASILFLVMLMLAASNEQTNIISIEPYYHNMYEESG
                     ESYEVALNNNTEVAVQFHATTSFNGFNIFFSGGLISRNPYLTVSLYEWNANYAKTIKG
                     NPVFTQKYFINKRGIWLEVNTPETKKGEYLLVVNDAYGGLNAEVVLPATPNTMTYVDR
                     TETYGSLMCTIKYINTPDKKLGAISENYREYNNTADTWVYTDGLGRKNPDYSKVRSKQ
                     KDKFVGIFYHTWHAKHSKVNSRNITEILKEHPEIKNNYSSPIWPKESTNYFWNEPLFG
                     Y"
     gene            1046..1903
                     /locus_tag="PLI11_05720"
     CDS             1046..1903
                     /locus_tag="PLI11_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52395.1"
                     /translation="MAYSESELLYYLEKSEVKSSIKLAGVRLFPAGYTYGPHKHDDIE
                     IVCVDSGVCIINFVADNKYVYVNKGEAIIIYPCVNHKFIIDEKAKCRITQIQFSLSDL
                     HDVEELSKSYEFINSLYSKKQYEVIDSCLDVKGCINRINNNMESSSLIMLYVLELVVH
                     LSISLTKIHDDTVKDKKLQRIIEYINMHLSEKIVIEDMCDVFGISSRYLRKKFKEAFG
                     VNVNHFITLQRINLAKKLLADRSLSVVKVACEAGFSSSQYFSHVFKKMVGVTPTEYRI
                     KHYRTKARF"
     gene            complement(1900..3609)
                     /locus_tag="PLI11_05725"
     CDS             complement(1900..3609)
                     /locus_tag="PLI11_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52396.1"
                     /translation="MCIAWRLLLIFITLSIVLLLFGCNKKANQVLLQKIADNEVEYDE
                     NVVPINWDNLDLNVLDSDIAKGLVQAINNSNRYAVNTWWTQVKKFDKQDDQEYISIGG
                     KSEATIRSISEMARLLAVSIKLNIYDESHTEVPLEEARAMMLKLVKSLAKAHLANTEG
                     GWGDHWQSAFWAADVAFSGWLFWENFGMEDQEMIRKVVVHEANRFISYEVPYYMDKEG
                     KIVFKGDTKAEENAWNANITTLACAMMPNHENHNLWMYKNLELIISSYALPEDVTSNK
                     LVNKFRLKEILDGSNINSDGTVVNHNIVHPDYQTSVLTQNMTKAAYFAFGGMEVPEAL
                     IFNADIIYSALITLDIGKFNADKKGSYIYERNPDGSASPEINYPTGTDWGVDRQLNFF
                     TSDVFAHVFNLDRDCPVKAIDYAHARMQVILSMQGRSDTGQYYQAGDSDSYALREEWV
                     AFHLINTYLVLWAENNGLVNISKDTFLKPAPLRSALPVLPEKIYVGNEFPISFEVNKD
                     LVVLPKDLKVKYYSSDESIVVIKNGVFKAVEVGQCKITVVVEYGKLRTSGTVSVTIND
                     YNY"
BASE COUNT         1157 a          794 c          743 g         1296 t
ORIGIN      
        1 aatatccaaa caagggttca ttccagaaat agtttgtgga ttcctttggc catatagggc
       61 tgctataatt gttcttgatt tcgggatgtt cctttagtat ttcagtaata ttcctgctat
      121 ttacttttga gtgttttgca tgccatgtat gataaaatat tccgacaaac ttatcctttt
      181 gttttgatct cacctttgaa taatctggat ttttccttcc aaggccatct gtataaaccc
      241 aagtgtctgc agtattgttg tactctctgt aattttcaga gattgcacct agttttttat
      301 caggtgtgtt aatatatttg attgtacaca tgagactgcc ataggtttcc gttctgtcca
      361 catatgtcat ggtatttggt gtggcaggga gtaccacctc tgcattcaga cctccataag
      421 catcgtttac aactaaaagg tactcacctt ttttcgtttc gggtgtgtta acttccagcc
      481 atatgcctct tttgttaata aagtacttct gtgtaaacac aggattgcct ttgatggttt
      541 tggcatagtt tgcattccat tcatacaaag acacggtcaa gtatggattt ctcgatatta
      601 gcccaccaga gaagaatatg ttaaatccat taaacgaggt ggttgcgtgg aactgaacag
      661 ccacttccgt gttgttgttt aaggcaactt cataagattc tccactttct tcatacatgt
      721 tgtggtaata tggctcgatt gaaattatat tggtctgttc gttgctggca gcaagcatca
      781 gcatcactaa aaatagtatt gaagcaatta caaaaaacag caactttctc atgacactcc
      841 tccataaatt tgtaggttgc attacttctc ttgatggcaa ttatatcttt gcaagatttt
      901 cagagtcgtt caattttcgg aacttattaa atatcctgtt caaaaaacat acaagacctt
      961 gcgaagcagc ttgtaaaggc atgttgcaga cagtataata caatcaaaag tatagaaaaa
     1021 tgcaatatat agtgagggga gtgccgtggc atattctgaa agcgagttgt tatattatct
     1081 tgaaaaaagc gaagtaaagt cttcaataaa acttgcagga gtacgccttt tccctgcagg
     1141 ttatacatat ggtccccata aacatgacga cattgagata gtgtgcgttg atagcggtgt
     1201 ctgcattatc aactttgttg cagacaacaa gtatgtttat gtaaacaaag gagaagcgat
     1261 tattatatat ccctgcgtta atcataagtt tatcatagat gaaaaagcta aatgccgcat
     1321 tacccaaatt cagttcagtc tttcagattt gcatgatgtt gaagaactgt ctaaatccta
     1381 tgagttcata aatagtcttt attcaaaaaa gcagtatgaa gtaattgatt cttgccttga
     1441 tgtaaaagga tgtataaacc gtataaataa taatatggaa tcaagctccc ttattatgtt
     1501 atacgttctg gaacttgtgg tccatctgtc aatcagcctg accaaaatcc atgatgacac
     1561 tgtcaaggac aagaaacttc agcgcattat tgagtatata aacatgcatt tgtctgaaaa
     1621 aatagtgata gaagacatgt gtgatgtatt cggaatcagc tcccgctact tgagaaagaa
     1681 gttcaaggaa gcctttggcg taaacgtaaa ccatttcata actcttcagc gcattaatct
     1741 tgcaaaaaag cttcttgcag accgctcact ttccgtagta aaagtggcat gtgaagcagg
     1801 ttttagcagt tcacagtatt ttagtcatgt gtttaagaaa atggttggcg tgacgccaac
     1861 cgagtacagg ataaaacatt ataggacaaa ggcgagattc taataattgt aatcgttaat
     1921 ggttacactt acagtgccac tggtgcgaag tttgccatat tccacaacaa cagttatttt
     1981 acactggcca acttcaactg ctttgaatac accattttta atgacaacta tactttcatc
     2041 actgctgtaa tatttgacct tcaaatcctt tggcagtaca accagatcct tgtttacttc
     2101 aaagcttata ggaaactcat tgccaacata tattttctct ggcaaaacag gcagggcaga
     2161 acgcaagggt gccggcttta gaaatgtatc tttcgaaatg ttgacaagcc cgttattttc
     2221 agcccataaa accagataag tgttaatcaa gtggaacgcc acccattctt ctctaagggc
     2281 ataggagtcg ctgtcgcctg cctggtaata ttgtcctgtg tcactgcgtc cctgcatcga
     2341 taaaattacc tgcatgcgtg catgggcata atctattgct tttacaggac aatccctgtc
     2401 cagattgaat acatgggcaa agacatccga ggtgaagaag ttaagctgtc tgtcaacacc
     2461 ccaatccgtt cccgttggat agtttatttc gggagatgcg cttccgtccg gattcctttc
     2521 atatatgtag ctgcctttct tatccgcatt aaacttgcct atgtcaagag ttataagtgc
     2581 gctgtagatt atatcagcat tgaaaatcag agcttccggt acctccatgc ctccaaatgc
     2641 aaaataggct gcttttgtca tattttgtgt cagcacggat gtctgatagt caggatgtac
     2701 tatattatga tttacaaccg ttccatcgct atttatattt gagccgtcta aaatttcctt
     2761 caacctgaac ttatttacca gcttgtttga tgtgacatct tccggtaaag cataggagga
     2821 tattatcagt tccaggttct tgtacatcca taaattgtgg ttttcgtgat tgggcatcat
     2881 cgcgcaggcc aaagtagtaa tgtttgcatt ccatgcattt tcttcagcct ttgtgtctcc
     2941 tttgaaaacg attttgcctt ccttgtccat gtagtaggga acttcgtatg aaataaagcg
     3001 gttggcttca tgcaccacaa cctttctaat catttcttga tcttccatac caaaattctc
     3061 ccaaaagagc catccggaaa atgcaacgtc agctgcccag aaggcgcttt gccaatggtc
     3121 gccccagcct ccttctgtgt ttgcaagatg tgctttggca agggatttta ccagcttcag
     3181 catcattgct ctggcttctt caagtggaac ttcagtatga ctttcgtcat atatgtttag
     3241 ttttattgaa acagcaagga gcctggccat ttcagatatg cttcttattg ttgcttctga
     3301 ttttcccccg atacttatgt attcttgatc gtcctgcttg tcaaactttt taacttgcgt
     3361 ccaccatgta tttactgcat atctgtttga gttgtttatc gcttgaacaa gtccttttgc
     3421 aatatcgctg tccagcacat tcaagtcaag attatcccag ttgattggga ctacattctc
     3481 atcgtattct acttcattat cagctatttt ttgcagtaat acttggtttg cctttttatt
     3541 gcacccaaag aggagtaaaa ctattgacaa tgtaataaat atgagtaata acctccatgc
     3601 aatacacata acaattttcc tttcacatta atgttgcttt ataaattata tcaactgtta
     3661 aatagtgtag tcgtgcataa atcggaattc gttaaaaaat atcgtacaaa ttatattcaa
     3721 ttccgttttt atcacaagta gttttcccta caaccgaaac acaaacgaca agtaatatat
     3781 gcagctatat actgggcgta gaaaaggttc aaaggaggaa ttataatgtg gagaagaatc
     3841 tttattcttg ttatgtgtgt catacatatt tgctgtatta tgatggttgg atgtgataga
     3901 aaaccggaag agcctgtccc gacagaaact acaaaagcaa gtacgccaac accaacacca
     3961 tcgccaactc cggaacctaa agaaaagtac
//
LOCUS       DAONVY010000176         3989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15922_length_3989_cov_3.669039, whole genome shotgun sequence.
ACCESSION   DAONVY010000176 DAONVY010000000
VERSION     DAONVY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15922_length_3989_cov_3.669039"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..2475)
                     /locus_tag="PLI11_05730"
     CDS             complement(22..2475)
                     /locus_tag="PLI11_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52397.1"
                     /translation="MRKYISLFLFVSVLFSLIFCRMVFHADGKVVDLYDSDGETDIFD
                     LEIGSTYAVKFHTDEVINEFHLYIENAPVNRSRLTLSLVPCDSTYGKTIRRQPEFTKT
                     YKKFGRDTWLVLNVDSLKAGEYLIVITPEQGNIKLRASIDNVDKAIFYENASALDIDF
                     YARVVFNTGNGEDLKNVSDFNNEFMAKPDTWACTDGLGRSLPLWEDAGKKREGKYVGL
                     FFHTWHSRFSAHSFADISRILKQNPNEKNYSNIDWGNKYSYFWSEPIWGYYDTRDRWV
                     LRKQAEMLADAGVDVIIMDNTNGTDIFKEGLLALLQVFSEARADGVKTPQVSFMLPMF
                     GPYEDTANQLRQLYDLIYSKGRYKDLWFYWKGKPLIMGCPDALMEYDPVDKEILDFFT
                     FRPANPSYREDHAQVFDEKGNVQVYIHPPKGYISWQWISVIPQQIAYNEDGTPEQVCV
                     SVAQNWSNEQGLTPMSFSEPEKLFGRSYSTLLKGHDKRENAHLYGKNFEEQWEYALKV
                     DPEFIFITGWNEWRAGLNEEIWGVRNAFADTFDDNNSRDIEPSKGELKDHYYYQMVSY
                     IRKFKGVSAPPRVSEPQTIDIHSEEDQWEKVTPYFASYPGNNIGRDYRGYGDMHYQQE
                     GSVNDIIGAKVARDDEYIYFMVETREKMQFGSNFMRLFIDVEGKELPDWETFEYVVNR
                     IAPGQKAVVERSLGGWNWEKVGEVEYSIKHNRLQLKIPKSMLGIEGDQFTINFKWSDN
                     MKEEGDIMDFYVNGNVAPGGRFKYRYSTVKYKSNSFGTWKLVLVVVLVLATAGLAFAV
                     GLRKRKKRGGALERSDRDY"
     gene            complement(2498..3715)
                     /locus_tag="PLI11_05735"
     CDS             complement(2498..3715)
                     /locus_tag="PLI11_05735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPZ52398.1"
                     /translation="MKILHVNVVYAVGSTGKIVYDIHKSLIDKGYESIVLYGREKKVK
                     ERNVYKTSTQMAVVYNALKSRVIGIPYAGSFFSTNRIIGIIKREKPDIVHLHCINGHF
                     VNIKRLMCFLKENNIKTVLTLHAEFMHTGSCPHAFDCERYMTGCGKCPYLWDATKSYF
                     FDFTAYAWRRMKSAFEGFNNLIIVASSEWILSRAQKSPITKDFEFCVIKNGIDTENIF
                     KPVPFEHLKEKHKLKDEKILLHVTANFDDPVKGSKYFVELARKLDGDNIKILLVGRNV
                     QDAHLPDNVIAVGGIYDQKELAAYYSMADLFVITSVRETYPTVCIEAVSCGTPVVGFD
                     TGGVKETIPEGMGMAVEAYSVDKLHEAVLEYIDKKDSIPKEVKEEARYRNSKERMARD
                     YLKVYTELYNRKI"
BASE COUNT         1082 a         1001 c          650 g         1256 t
ORIGIN      
        1 actataccga attttcagga attaataatc cctatctgac cgctccaaag cacctcccct
       61 tttcttcctc ttcctcaatc cgacagcaaa ggccaaacca gcagttgcaa gcaccaaaac
      121 aacaactaat acaagcttcc atgtgccaaa gctatttgat ttgtatttta cagtcgaata
      181 cctgtacttg aatcgtccac caggagcaac atttccattc acatagaagt ccattatgtc
      241 tccttcttcc ttcatgttgt cggaccactt gaagttgatt gtaaattgat ccccttcaat
      301 ccccagcatg gactttggaa tttttagctg cagtcggttg tgtttaatgg agtactccac
      361 ctctccaacc ttctcccagt tccagccacc caaagacctt tcaaccacag ccttctgccc
      421 cggagcaatc ctgttgacaa catactcaaa agtctcccag tcaggcaatt ccttgccttc
      481 gacatctata aacagccgca tgaaattgct gccaaattgc atcttttccc tggtttccac
      541 catgaagtat atgtattcat catctcttgc cacttttgca ccaatgatat cattaacact
      601 gccttcctgc tggtagtgca tgtctccgta acctctgtaa tcccttccaa tattgttgcc
      661 aggatacgat gcaaaatatg gggtcacttt ttcccactgg tcctcttcgg agtgtatgtc
      721 tattgtttgg ggttcggaca cccttggcgg cgcggatacc cctttgaact tgcgaatata
      781 tgataccatc tggtagtaat agtggtcctt caactctcct tttgatggct ctatgtccct
      841 gctgttgttg tcgtcaaagg tgtctgcaaa ggcattcctt actccccaga tttcctcatt
      901 taatcctgcg cgccactcat tccatccggt tatgaagata aattcagggt ccactttcaa
      961 agcgtattcc cattgttctt caaagttctt tccgtacaaa tgagcattct ccctcttgtc
     1021 atgccctttt agcagtgtgg aataggatct gccaaaaagt ttctcaggtt cgctaaaact
     1081 catgggtgta agcccttgtt cattgctcca gttctgggca acggaaacgc acacctgctc
     1141 cggtgttcca tcctcgttgt aagctatctg ctgtggaatt acggatatcc actgccagga
     1201 tatatatccc tttggcggat gaatatatac ctgtacattt cctttttcgt caaatacctg
     1261 tgcatggtct tccctgtatg aagggtttgc tggccggaaa gtgaagaaat caagaatctc
     1321 cttgtccaca gggtcatatt ccatcaaagc atcaggacaa cccataatca agggctttcc
     1381 tttccagtaa aaccataaat ccttgtatct tccctttgaa taaatcaaat cataaagctg
     1441 tctcaattga tttgcagtat cttcgtatgg accaaacatc ggcagcatga aggaaacttg
     1501 tggtgttttg actccatccg cccttgcttc agaaaagacc tgaagcagag caagaagtcc
     1561 ttccttgaat atatcggtgc cattggtgtt gtccattatg ataacgtcaa cacctgcatc
     1621 agccagcatc tctgcctgct ttctaaggac ccatctatcc cttgtatcgt aatagcccca
     1681 gatgggctcg ctccagaagt aggaatactt gttgccccaa tcaatatttg aatagttttt
     1741 ctcatttggg ttctgcttaa gtatccggga tatgtctgca aagctgtggg cagaaaatct
     1801 tgaatgccat gtatggaaga acagcccgac atactttcct tctcttttct ttcctgcatc
     1861 ctcccataaa ggaagacttc tgccaagacc gtcggtacat gcccatgtgt caggcttggc
     1921 cataaactca ttattaaaat cagacacatt tttcaaatct tcaccattcc ctgtattaaa
     1981 tacaactctt gcataaaaat cgatatccaa agcggatgca ttttcataga atattgcctt
     2041 atccacattg tcaatacttg cccttagctt gatattccct tgttccggtg taataacaat
     2101 gaggtactct cccgctttta agctgtctac attaagtaca agccatgtat ctcttccaaa
     2161 cttcttatat gtttttgtaa attccggctg tcttcgtatt gtttttccat aagttgaatc
     2221 gcatgggacc aaactcaatg taagtctgct gcggttgacc ggggcatttt caatatacaa
     2281 atggaactca ttgattactt catctgtatg aaactttaca gcatatgtag agccaatttc
     2341 cagatcaaaa atgtctgtct ctccatcgga gtcatacaaa tccaccacct tgccatccgc
     2401 atggaaaacc atccggcaaa aaatcaatga aaacaagaca gacacaaaaa gaaacaagct
     2461 tatgtacttc ctcatactct tacctcctct tacctctcta tatctttcga ttatatagtt
     2521 ccgtatacac tttaagataa tcccttgcca ttctctcctt gctgtttcta taccttgctt
     2581 cttctttgac ttccttgggt atactgtcct ttttgtcaat atactcaaga accgcttcat
     2641 gaagcttgtc aacgctgtat gcctccactg ccatccccat tccttcgggg atggtttcct
     2701 ttactccacc ggtatcaaac cccaccactg gagttccgca ggatacagct tctatacata
     2761 cggtgggata cgtctccctt acacttgtta tgacaaacaa gtctgccatg gaatagtaag
     2821 ctgcaagctc tttctgatca tatattccac caacagctat tacattgtca ggaaggtgtg
     2881 catcttgcac atttcttccc acaagcaaaa tcttaatatt atctccatca agctttctgg
     2941 caagctcaac aaagtatttg ctgcctttta cagggtcatc aaagtttgca gttacatgaa
     3001 gaagtatctt ctcatccttt agtttgtgct tttctttcaa atgctcaaaa ggaaccggtt
     3061 tgaatatatt ctcagtgtct atgccgtttt tgataacgca aaattcaaaa tcctttgtta
     3121 ttggagactt ttgggcacga cttaaaatcc attccgaaga agcaacgata atcagattgt
     3181 taaagccttc aaaagcactc ttcattcttc tccaggcata cgctgtaaag tcaaagaaat
     3241 atgactttgt tgcatcccac aaatacggac acttgccgca acctgtcata tatctctcac
     3301 agtcaaaagc atgaggacaa gaacctgtat gcatgaattc tgcatgcaga gtcagcaccg
     3361 tctttatatt attctcctta agaaaacaca taagcctctt gatatttaca aaatgcccgt
     3421 ttatgcagtg aagatgtaca atatccggtt tctcccgctt aattattcca attatcctgt
     3481 ttgtagaaaa aaagcttcct gcataaggaa tccctattac ccgagacttt aaagcattgt
     3541 atacaaccgc catttgggtt gaggttttat acacattccg ctcttttacc tttttttctc
     3601 ttccgtaaag aacaatggat tcataaccct tgtcaattag agatttatga atgtcatata
     3661 caattttacc agtactgcca acagcataaa ccacattcac atgcagtatc ttcataacat
     3721 tcctctttca tcttttctac accatattat atcccacgct caaaaaaatt taaaggtaga
     3781 aaaaaggcag cctgtttaaa gaccgccttt taaactttta aaattcactt attccacttt
     3841 tgtcaatcct ataagttgca aactcacgag cttttacaat cacgcaagat tccacatcca
     3901 tagcaggtag cttaatcttt actatctggt caacacccga tggattaaac agtctgagaa
     3961 tatatttctt tccgtcatct gccagtctg
//
LOCUS       DAONVY010000177         3971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_15993_length_3971_cov_3.911645, whole genome shotgun sequence.
ACCESSION   DAONVY010000177 DAONVY010000000
VERSION     DAONVY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15993_length_3971_cov_3.911645"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="PLI11_05740"
     CDS             complement(<1..2002)
                     /locus_tag="PLI11_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HPZ52399.1"
                     /translation="MVYLAVAGIIKAEDAIDEICIQGDKYHDLDRHKGYREYRGSVKT
                     EQIYDEFPFVLSDQTSLVLVGFAESINDGAYGDGPEIVDTGELLKDAKTYGIKKLIAY
                     LWTIGINIDWNEYYDGKRHNRIIAPTYPFERKSYFIEPMANNEVAADGTFYNKCMVKE
                     PEVKADCSGDEIISYEAVQAKLVEIWEKALGQKINNFQNSFFELGGHSLLATQVLFTV
                     CREFKIEIPLQKFFENPTIPGFTNTLLQYLIARESRYEPLPEVETDLENRYEPFPLTD
                     VQKAYWVGRNDKMELGNTSTHMYFENDIKQLDIERFNIAFNKMIERHEMLRAVMLPDG
                     TQKILKEVPKYNIKVNNMANSSVEEIRRYMDTIRNEMSHQVLDCYNWPLFDVRATLLP
                     DDCVRLHISIDLLITDAWSLELLLNELSILYHKPDYVFEKLDLSFRDYVISLNKIENT
                     QNFVRSKEYWQKRLDTLPPGPQLQLAKDPDTIITPVFRRWKYILKKENWKKLKMKASL
                     LGITPTVMLLSAFSEVLSTWSKSSHFTLNLTLFNRLPMHPQVNEVIGDFTSLTLLEVN
                     YSEVEPFIKRVRANQVQLLKDMDHRYYSGVRVTRDLIAKYKDPARAIIPVIFTSLLNQ
                     VEATWEDPDNAFTSNIGNRNDDRYSISQTPQVWLDHQVIEKGG"
     assembly_gap    2023..2032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2033..3880)
                     /locus_tag="PLI11_05745"
     CDS             complement(<2033..3880)
                     /locus_tag="PLI11_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HPZ52400.1"
                     /translation="MENKNERMKFDMDSNKELYINSSNDIAIIGMVCRFPGVKTVDEF
                     WEVLRDGVEVIHRDTDKYYQVNYLSDMIHVNTVIEDAEMFDANFFGFTAREAEITDPQ
                     HRLFLEGSWELMERAGYNPIDYKGLIGIYAGVSTSTYLINNILSRKDIMNSVGDLQLM
                     IGNDKDHLTAQVAYKLNLKGPCVTVQAACSTSLVAVHLACESLLSGQCDMAIAGGITI
                     KYPQLPGYKFQQGGLVSDDGHLRPFDGNATGTVYSNGMGIVLLKRLQDAINDGDQIYS
                     IIKGTAVNSDGSSRVGYTAPGVDGQVAVITEAQSVAGVSPEDITYIEAHGSGTPLGDS
                     IEIEAMIRAFSLNTDKKEFCAIGSVKANIGHTEMASGVAGLIKTALALKHKKIPPSLN
                     YDEPNPKLGIYETPFYVNTMLTDWDTDCLPRRAGVSSFGLGGINCHVVLEEAPETEAK
                     NNDEETEFLVLSAKTPTALKKYISNMLTYLRLNPEVNLSDLAYTMKVGRNSFPYRKAF
                     VFKDYGHLMEMLNNCEKAIDTDVKCVSSRTAEFLFSSKPSAKWEDVMDLYNEVSEYHQ
                     IFDRLLAYIRAKYGIILIEENEKYDVLSSFVIQYTIGTLLTKWGIKIETA"
BASE COUNT         1146 a          891 c          588 g         1336 t
ORIGIN      
        1 cccctccctt ctctattacc tggtggtcca gccatacttg gggagtctgg gatatgctat
       61 accgatcatc atttctgttt ccaatattgc tagtaaaggc gttatcaggg tcttcccacg
      121 tagcttcaac ctgattcaat aaacttgtaa atataacagg aataattgct ctggcaggat
      181 ccttatattt tgcaataagg tctctggtaa ctcttacacc actgtaatac ctgtggtcca
      241 tatctttaag caattgtacc tgattggcgc gcactctttt aataaaaggc tctacttcag
      301 aataattgac ctcaagcaat gttagagatg taaaatctcc gatcacttca tttacctgag
      361 gatgcatagg aagacgatta aacagggtta gatttaacgt aaagtgcgaa cttttgctcc
      421 atgttgacag tacctccgaa aaagctgaca gcagcataac tgttggagtt atccctaaca
      481 acgaagcttt cattttcagt tttttccaat tttccttttt gagtatatat ttccatcttc
      541 taaatactgg agttattatg gtgtctggat cctttgccaa ttggagttgt ggacccggcg
      601 gcaaagtatc taacctcttc tgccaatact cttttgacct tacaaaattt tgtgtgttct
      661 ctattttatt aagactaatt acataatctc taaatgaaag atccagtttt tcaaatacgt
      721 aatcaggttt atggtataaa atagataatt cattaagcag caattccaga ctccaagcat
      781 cagtaataag aaggtctata cttatatgaa gccttacaca atcgtcagga agcaatgttg
      841 cgcgcacatc aaacaatggc cagttataac agtctaacac ttgatgtgac atttcatttc
      901 ttattgtatc catatacctt ctgatttctt caacactgct gtttgccata ttgttaactt
      961 ttatattata cttgggaacc tctttcaata tcttttgagt tccatctgga agcattactg
     1021 ctctaagcat ttcatggcgc tctatcattt tgttaaaagc aatattaaat ctttcaatat
     1081 ccagctgctt gatatcattc tcaaaataca tgtgagtcga agtattaccc agttccattt
     1141 tatcgttccg tccaacccaa tatgccttct gcacatcagt aagaggaaat ggctcatatc
     1201 ggttttccaa atctgtttca acttccggta aaggttcata cctactttct ctggcaataa
     1261 gatactgtaa caaagtattt gtaaaccctg gtattgtagg attttcgaaa aatttttgca
     1321 atggtatctc aattttgaat tctctacata ctgtaaaaag tacttgtgta gcaagaagcg
     1381 aatgtccacc aagctcaaaa aaactatttt gaaaattatt aatcttttgt ccaagggctt
     1441 tttcccatat ttccacaagc tttgcttgta ctgcttcata tgaaataatc tcatcaccag
     1501 aacaatcagc ttttacttca ggctctttta ccatacattt attataaaaa gttccatctg
     1561 cagcaacctc attattggcc ataggctcta tgaaataact ttttcgctca aacgggtagg
     1621 taggagcaat aatcctatta tgcctttttc cgtcatagta ctcattccag tcaatattga
     1681 ttccaatagt ccataaatat gcaattaact ttttaattcc atacgttttt gcatccttta
     1741 aaagttcacc agtatccact atttccggac catcaccata agctccatca ttgatagatt
     1801 ctgcaaagcc tactagaact aaagaagttt gatcgcttaa tacaaacgga aactcatcat
     1861 atatttgttc tgtcttaaca gatcccctat attctctata gcctttatgc ctgtccaaat
     1921 catgatattt gtccccttgg atgcatattt catcgatagc atcttctgcc ttgattatcc
     1981 cggcaacagc aagatatacc aattctccta ctccttgcac atnnnnnnnn nnggcagttt
     2041 caatctttat cccccatttt gtcaataagg ttccaattgt atactgaata acaaagctag
     2101 acaagacatc gtatttttca ttttcctcaa ttaaaataat cccgtattta gctcttatat
     2161 acgctaaaag cctgtcaaat atttgatggt actcgcttac ttcattataa agatccataa
     2221 cgtcttccca cttagcagaa ggtttactag aaaataagaa ttcggctgtt ctgctactta
     2281 cacactttac atcagtgtca attgctttct cacaattatt gagcatttcc atcagatgcc
     2341 cataatcctt aaaaacaaaa gcctttctgt aaggaaaact gtttcttcca actttcattg
     2401 tatatgccaa atccgataga ttcacttcag gattaagcct tagatatgtc agcatatttg
     2461 aaatatattt cttcaaggcc gttggtgttt ttgctgataa tacaagaaat tcagtttcct
     2521 catcattatt tttagcttca gtttcaggtg cttcctcaag aacaacatgg caattaattc
     2581 ctcccagtcc aaaagaactg acacctgctc ttctcggtaa gcaatcagta tcccagtctg
     2641 tcagcatggt attgacatag aagggagttt catatattcc gagcttagga ttcggttcat
     2701 cataatttaa actcggcgga attttcttat gttttagagc aagtgcagtt ttgataagtc
     2761 ccgcaacccc tgaagccatt tcagtgtgtc caatatttgc ctttactgaa ccaattgcac
     2821 aaaattcttt cttatcagta tttaaagaaa aagcgcgtat cattgcttct atttcaatgc
     2881 tatcacccaa tggtgtacca cttccatgag cttctatata ggttatatcc tcaggtgaaa
     2941 ctcctgcaac actttgagct tcagtaataa cagctacctg accgtctact ccaggagcag
     3001 tataacctac ccttgatgaa ccatcactat ttacagctgt ccctttaata atggaatata
     3061 tctgatctcc atcatttata gcatcttgta atctttttaa aagcacgatt cccattccat
     3121 tgctgtaaac ggttccagtt gcattgccat caaacggccg taagtgacca tcatctgaaa
     3181 ccagcccacc ttgttggaat ttatatccgg gtagctgtgg atatttgata gttatccctc
     3241 ccgcaatagc catgtcacat tgtccactta ataaactttc gcatgccaga tggacagcca
     3301 caagggaagt cgagcaggct gcctgtactg taacacaagg gcctttaagg tttagcttgt
     3361 atgccacttg tgctgtgaga tgatctttgt catttccaat catcaactga agatctccca
     3421 cagaattcat tatatcttta cggctcaaaa tattattgat aagatatgta ctcgtgctta
     3481 cacccgcata aattcctatc aaccccttat aatcaatcgg gttgtatcct gccctttcca
     3541 tcaattccca cgaaccttcc aaaaacaacc tgtgctgtgg atcagttatt tctgcttctc
     3601 ttgctgtaaa accgaagaaa tttgcatcaa acatttctgc atcttcaatt acggtattta
     3661 catgtatcat atctgaaaga taattaacct gataatattt gtcagtatct ctatgaatta
     3721 cttccacccc atctcttagt acttcccaaa actcatcaac tgtcttcact cccggaaaac
     3781 ggcagaccat acctataata gcaatatcat tcgaactgtt tatatacaac tccttattac
     3841 tatccatatc aaacttcatc ctctcattct tattttccac gtgttctcct tgaattcaga
     3901 cgcattgccg cacggctcac tgcactttct acaagttttt tatcattact tccttgattt
     3961 agatattctg c
//
LOCUS       DAONVY010000178         3962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16033_length_3962_cov_2.487330, whole genome shotgun sequence.
ACCESSION   DAONVY010000178 DAONVY010000000
VERSION     DAONVY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16033_length_3962_cov_2.487330"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /gene="nadB"
                     /locus_tag="PLI11_05750"
     CDS             <1..1477
                     /gene="nadB"
                     /locus_tag="PLI11_05750"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPZ52401.1"
                     /translation="EGVDISSSVLAQGGIAVSLDKSDSPDIHMQDTLYAGAGLCDEES
                     VRVLVDEAAENINLLCKYGVEFDRSPEDKRKLALTREGAHSKNRIIHAGDATGKEVCD
                     KLIYNVTKRSNVTIYEKTWALDLLVKDGACHGLIAMEEETEKPIVFYADAVICATGGY
                     GQLYYYTTNPVVATGDGAAFTFRAGGELEDLEFVQFHPTVLYHPENKSFLISEAVRGE
                     GAILRNAKGERFMPNYHELAELAPRDVVSRCIFMEMKKTGTENVYLDITHEGKDYLEK
                     RFPNIYSTLQKYGMDMSKDHIPVAPAEHYCMGGIKTNLDGETNIINYYACGEAACTGI
                     HGANRLASNSLLEGIVFGRRIALKVSEMLMNEKNNQKHHARFFVKTERMNKGYNSKEA
                     ILSIKQTMTELVGIERTYDTLNEALQRVVEIKKEIADMNNTTFDDYVLQNMVLLAELV
                     IKAAIMREESRGAHYRSDFKETKDEWKVHIVFSKDNIVREE"
     gene            1486..2334
                     /gene="nadC"
                     /locus_tag="PLI11_05755"
     CDS             1486..2334
                     /gene="nadC"
                     /locus_tag="PLI11_05755"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPZ52402.1"
                     /translation="MNEVYLDMKQVDKVILTALEEDMPFGDITTDNIIAGSSISKARL
                     IAKEDGVLAGVPVAVRTFELLSRDIKVEVLLQDGAKVKKGDIIANIEGPTREILKGER
                     TALNLLQRLSGVATATNKLVELVKDYPVNVVDTRKTTPGLRYLEKYAVRVGGGQNHRF
                     SLSDGVLIKDNHISAAGGIKNAVEMVRRRIPHTVKIEVETENLEMVREALDVGADIIM
                     LDNMTNEMMSEAVKLINKKALVEASGDINEQRIVEVTKTGVDIISVGRITHSVKALDI
                     SLRFYN"
     gene            2430..3548
                     /gene="spoIID"
                     /locus_tag="PLI11_05760"
     CDS             2430..3548
                     /gene="spoIID"
                     /locus_tag="PLI11_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HPZ52403.1"
                     /translation="MFKLNRHIISYAVLSIILSALVVLSIVSGNAKTPLTSRNFHSII
                     GLIRSQLDNVPKTDDNDIQTAVGSISTDDLDDLLIRVYDSGEKRVREMFLEDYIMGVV
                     YAEMNLRNNIEALKAQAVAARSYTLYKFYNDSEGVNIHKDGAIICTDYTHCQAWKDPE
                     GIIPAYGKETGQELYNKLKKAVESTKGIVMTYEGEVIKAMYFSSSGGYTESMEDVWYE
                     KLEYLKSVPSYGELANDYYCNFAYFSKSDFVARFKKAYGYNFKAEPYNIMESITNIER
                     SSTGRIKKITIGGVEMSGIAARSILGLRSTNMIFREFNKDTILIITIGTGHGVGMSQL
                     GAITMADEGKTYDEILKHYYSGVELERELYTSIGLPSN"
     gene            complement(3560..3799)
                     /locus_tag="PLI11_05765"
     CDS             complement(3560..3799)
                     /locus_tag="PLI11_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52404.1"
                     /translation="MLKIDPDKYEEMPPSKVEFQYPSAFIDNDILYVLLRTAFNHAFN
                     YHDANYITFHKYLKNIKGKAAWQAINAIPCRLFQD"
     gene            complement(3780..>3962)
                     /locus_tag="PLI11_05770"
     CDS             complement(3780..>3962)
                     /locus_tag="PLI11_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52405.1"
                     /translation="SIHASGVYIYNSGFLKHNPNWANAEDISGAIEGNIVVAPDGSIV
                     NMLRYSKNKANAKNRS"
BASE COUNT         1340 a          657 c          940 g         1025 t
ORIGIN      
        1 agagggagta gacatatcaa gctcggttct tgcacagggc ggtattgcag tttctcttga
       61 taaaagcgac tctcctgaca tacacatgca ggacaccctt tatgcaggcg caggcttgtg
      121 cgatgaggaa agtgtaagag ttctcgttga tgaggcagct gaaaatatta acctattgtg
      181 caagtacgga gtggagtttg atagaagccc tgaggataaa agaaaactgg cacttaccag
      241 ggagggtgca cacagcaaga acagaataat acatgccgga gatgcaaccg gtaaagaagt
      301 atgtgacaag ctgatataca atgtgaccaa gagaagcaat gtcacaatct atgaaaaaac
      361 ctgggctctg gacctccttg taaaggatgg tgcctgccat ggccttattg ccatggaaga
      421 ggaaacagaa aagcccatag tgttctatgc cgatgcggtg atatgtgcta caggcggcta
      481 tggacaactt tactactata ccacgaatcc agttgtggcc acaggagatg gggctgcttt
      541 tacattcaga gccggcggtg aactggaaga tctagagttt gttcagtttc atccaacggt
      601 tttgtatcat cctgaaaaca aaagttttct catatctgag gcggttagag gagaaggcgc
      661 tattttgcgc aatgcaaaag gcgaaagatt catgcctaac tatcatgaac ttgcagaact
      721 tgctccaaga gatgtggtat cgcgctgcat ttttatggag atgaagaaaa caggtactga
      781 aaatgtgtat ttggatataa cccatgaggg caaggattat ctcgaaaaaa gatttcccaa
      841 catctattcc acattacaaa aatacggtat ggatatgtca aaagaccata taccggttgc
      901 tccagcagaa cattattgca tgggtggtat taagacaaat ctggacggag aaacaaacat
      961 aataaactat tatgcgtgtg gggaagctgc atgtacagga attcacggag caaacaggct
     1021 tgcgtctaat tcccttcttg aaggtatagt ttttggacgc agaatagctt taaaagtatc
     1081 tgaaatgctt atgaatgaga aaaacaatca aaaacatcat gcaaggttct ttgtaaagac
     1141 cgaaaggatg aataaaggct ataattcgaa agaagcaatt ctttcaataa agcagacaat
     1201 gacggagctg gttggtatag aaaggaccta tgatacttta aacgaagcac tgcagagggt
     1261 agttgaaatt aagaaagaaa ttgcagatat gaataacaca acctttgacg attatgtact
     1321 tcagaatatg gtcttgttgg ctgagttggt tataaaagcg gctatcatga gggaagaaag
     1381 ccgtggagcc cattacagat cagactttaa ggaaacaaaa gacgagtgga aggtgcatat
     1441 tgtattttca aaggataata ttgtacgaga ggagtaatag tagctatgaa tgaagtatat
     1501 ttggacatga aacaggtaga taaggtaatc ttgacggctc ttgaagagga tatgcccttt
     1561 ggagatataa caacagacaa cataattgca ggaagcagta tttcaaaagc tagactgatt
     1621 gcaaaggaag acggcgtact ggcaggtgtt cccgttgcag ttaggacatt tgagcttttg
     1681 agtagagata taaaagtgga ggtcttattg caggacggag caaaagtcaa aaaaggcgac
     1741 attatagcaa atattgaggg accaacgaga gagatattaa agggcgagag aacggcttta
     1801 aatcttttgc agagactatc cggtgtggct actgccacga acaagcttgt ggagcttgtt
     1861 aaggattatc ctgtaaatgt tgtggataca agaaaaacca cacctggtct tagatatttg
     1921 gagaaatatg cagtgagagt ggggggagga cagaaccaca gattctctct ctcggatgga
     1981 gtacttatta aagacaatca tatctctgcc gcaggaggta taaagaatgc agttgagatg
     2041 gtaagaagaa gaattcctca cacagtaaag atagaagttg agaccgagaa tctcgaaatg
     2101 gtccgagaag cactggatgt tggtgcagat atcattatgc ttgataacat gacgaatgag
     2161 atgatgtcgg aggctgtaaa gctcataaat aaaaaagccc tggttgaagc atctggtgat
     2221 attaatgagc agagaatcgt agaagttaca aaaaccggag ttgacataat ttctgtagga
     2281 aggattacac attcagtaaa agcactggat ataagtctta gattctacaa ctaaacattc
     2341 tttgttggca attattagat agaaaattga ataaagtggc aataacagag gaataatata
     2401 cctgtaatga tgaatatgga ggtgttctcg tgttcaaact taacaggcat attatatcct
     2461 atgctgtttt atccataatt ctgtcggcac ttgtagtact tagcatcgta tcaggaaatg
     2521 ccaaaacgcc tttaacaagc aggaactttc atagcataat aggccttatc aggtctcaat
     2581 tggacaacgt gccaaagaca gatgataatg acatacaaac agctgtgggg agtattagta
     2641 cagacgacct ggacgatttg ttgataagag tatatgactc cggagaaaaa agagtcagag
     2701 agatgtttct cgaggattac attatgggcg ttgtttatgc agagatgaat cttagaaaca
     2761 acattgaagc actaaaggcc caagcggttg cagccaggag ctatactctg tacaaattct
     2821 acaatgatag cgaaggagtc aatatccaca aggacggagc aattatttgt accgattata
     2881 cccactgcca ggcatggaaa gatcccgagg ggataattcc cgcctatggc aaggagaccg
     2941 ggcaggaatt gtacaacaaa ctgaagaagg ctgtggaatc gacaaaaggc atagttatga
     3001 cgtatgaagg agaagtcata aaagccatgt atttttccag cagcggtggt tatactgaaa
     3061 gcatggaaga cgtatggtat gagaagctgg agtatttgaa aagtgtaccg agttatgggg
     3121 aacttgcaaa cgattattac tgcaattttg cgtactttag taaaagtgat tttgtagcaa
     3181 gattcaaaaa agcatatgga tacaacttca aggcagaacc gtacaatatt atggaatcaa
     3241 taaccaatat tgaaagaagc tctacaggac gcatcaaaaa aatcaccata ggtggtgtag
     3301 aaatgtccgg cattgctgca cgttcaatac tgggattgcg atcaacaaac atgattttta
     3361 gagagtttaa caaggataca atattgataa taaccatagg taccggacat ggcgtaggaa
     3421 tgagtcagct tggagcaatt acgatggcag atgaaggaaa aacatacgat gaaatattaa
     3481 agcactacta ctccggagta gaacttgaaa gagagttgta tacaagtata ggactacctt
     3541 ccaactagca cttcatttat caatcctgga aaagccggca tggtatggcg ttaattgcct
     3601 gccatgccgc ttttcctttt atatttttca agtacttgtg aaatgttatg taatttgcat
     3661 cgtgatagtt gaaagcatga ttgaaagcag ttcttaaaag gacatataga atatcgttat
     3721 cgataaatgc agaaggatat tgaaactcca cctttgacgg aggcatttct tcatatttgt
     3781 caggatcgat ttttagcatt tgccttgttc tttgagtatc tcagcatgtt tacaatagaa
     3841 ccatctggag ctacaacaat attaccttct atagccccgg aaatgtcttc agcatttgcc
     3901 caattgggat tatgttttag aaaaccactg ttataaatgt agacacctga tgcatggatt
     3961 ga
//
LOCUS       DAONVY010000179         3950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16092_length_3950_cov_2.533761, whole genome shotgun sequence.
ACCESSION   DAONVY010000179 DAONVY010000000
VERSION     DAONVY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16092_length_3950_cov_2.533761"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLI11_05775"
     CDS             complement(<1..515)
                     /locus_tag="PLI11_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52406.1"
                     /translation="MLESFRKHKKILLLLSGLILIVILTLMVMSQNKNSKFSDVNLTP
                     DRITKIHIRMMAPIDKYSTDQFVIEEEENIETVLDYVKKMSVVEVVKEDFGIKPGAYE
                     FTYYMKDGKVKRYVHEFLAVTSDDGFENIFKLKEVIPQINYIFTTKKEDVREVALFGW
                     KNENDREAERA"
     gene            complement(551..1381)
                     /locus_tag="PLI11_05780"
     CDS             complement(551..1381)
                     /locus_tag="PLI11_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52407.1"
                     /translation="MFKNRILIVIAVFLLIFGTATIATYSGYRNSRAANMKKDAEIDN
                     YGMYINKRAKGARNIVYSDERVKLKYDRSENLSSRKPEDRSDAYGSYDVYVDDNGNEF
                     YYLQNTDMLCGVKNKKIDGERLTPFDNDDEAIKIAEEFMIKIFGSDHDYDFESCKIPP
                     QRFYYEVIYRKYVNGYRTDDYVRLWVNFDGEVCAFSAFNRDRYDHITINRPSAIASQQ
                     RSKSNIVDTLNSENFTIVDQYISKNEEGKLVMVSVIEYSLTDGVSVYPIKDEVSVVIE
                     "
     gene            complement(1396..2568)
                     /locus_tag="PLI11_05785"
     CDS             complement(1396..2568)
                     /locus_tag="PLI11_05785"
                     /inference="COORDINATES: protein motif:HMM:NF025563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HPZ52408.1"
                     /translation="MQKKICMVLLFAMMFSFLGFNGYAAMAATNDVPGLETGEVYYIR
                     NVITGRYLEVQNGADSNGTPVISSEGNRIYAQQWKITRNSNGTYKLQPMSSPTRKVLD
                     ITGNNVDIWTDNGASYQQFTIDRISTTHDPKINGCYHIKYNGRFLATNGFGTVYLSFL
                     NLGDGTAWSFEKVTKHDADIYSQIILVDWILFVPNYFDTRGADKTFVDTMTAKGYYAF
                     KLNNATASFAYHYLQRDAVWVFVGMTTVDEMGNPCAVICFANEENESLGNIAASRSLF
                     NGASTYTIEEIDANGLARAKIVIYLAAYTDADIMLGSRKYGLIRDTYDKGAHFVVGVN
                     EKCSHLGATYWAKLFFIRAGWSSDATIKECVEYADYSAYQAGTINTTYYGDVNSRV"
     gene            complement(2726..>3950)
                     /locus_tag="PLI11_05790"
     CDS             complement(2726..>3950)
                     /locus_tag="PLI11_05790"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPZ52409.1"
                     /translation="ERKYARCLKYTLALLTLLVRVVFFYFIPTFALNIILSFVLAYAF
                     LAIVFKGDTIDKLLTIVAYILYILIAELIILQFQNMFLINPLYLNDISYQLIYITTTR
                     TILFWLCLTNANIVNRRFSHIPLYYWVIIITVPLASVIMLNTVYWSIIYSMDKYALYL
                     GISIICLLYMNFSIFAFIDSYTTNVKVTVLESLIEKENENYKQLGFSYDEMRKMRHDF
                     KNHIVMIESLIKNNEIRLLHKYIKDFEAEIEDASMIYTKNPAIDAIINIKGLLAKEND
                     IRYVVKTSNIIDNIYISPYNVCRILGNAIDNAIEACMRIKDETLEKFIFISFQRIENN
                     IVILIENSADRVKKTLTDQFESLKDNKSMHGLGLSIIRDAVQELGGFMNVEFQNSVFT
                     LHVVIPYKGSASKTG"
BASE COUNT         1090 a          830 c          689 g         1341 t
ORIGIN      
        1 atagcccttt cggcctctcg gtcattttca tttttccaac caaacaaggc tacttctcta
       61 acatcctctt tcttggtggt aaatatgtag ttaatctgtg gtataacttc tttcagttta
      121 aatatattct caaatccatc atcagaggta actgctagaa actcatgtac ataccttttg
      181 accttgccat ccttcatgta gtaggtaaat tcataagcgc ctggttttat tccgaaatcc
      241 tcttttacta cctccaccac agacattttc tttacataat caaggaccgt ttctatgttc
      301 tcttcttctt caatcacaaa ctggtctgtt gaatacttgt ctataggcgc catcattcta
      361 atatgtattt tcgtaattct gtctggagta agatttacat cagaaaattt actgttttta
      421 ttttgcgaca tcaccatgag ggtcaatatg acaatcagga ttaagccgct aagtagcagc
      481 agtattttct tgtgttttct aaagctttcc aacataaata tcgtctccag ttatcttttg
      541 taaaatttaa ttactcaatt accacagaca cctcatcctt aatcggatac acactaactc
      601 cgtcagtcaa tgagtactca atgaccgaaa ccatgaccag ctttccttct tcattcttgg
      661 aaatgtactg atccacaatt gtaaaattct cagagtttag cgtgtcaaca atattgctct
      721 tgcttctttg ttgtgaggca atagctgaag gtctattgat tgttatgtgg tcgtatctat
      781 ccctgttaaa agctgagaat gcgcatactt ctccatcgaa gtttacccaa agtcgtacat
      841 agtcgtcggt cctgtaaccg tttacatact tgcggtatat tacttcgtag taaaaccttt
      901 ggggaggaat cttacatgat tcaaaatcat aatcatgatc tgaaccaaaa atcttaatca
      961 taaactcttc agctattttt attgcctcat catcgttatc gaagggtgtc aacctttctc
     1021 catctatctt tttgttttta actccgcaaa gcatatcagt gttttgcaga tagtaaaatt
     1081 cattaccatt atcatctacg tatacatcat aggaaccata agcatcgctt ctatcttcag
     1141 gcttcctgga gctcaggttt tctgatctat catatttaag tttgactctc tcgtcagaat
     1201 atacaatatt cctggctccc tttgcccttt tgtttatgta cattccgtaa ttgtcaatct
     1261 cagcatcctt tttcatattg gcagcccttg aatttctgta accgctgtat gtagcaatag
     1321 tggctgtacc aaatatcaat aaaaaaactg caatcacaat aagaattcta ttcttaaaca
     1381 tcccaaaccc ctcctctaca cacggctgtt gacatcgcca tagtatgttg tgttaatggt
     1441 tcctgcctga taagcgctat agtcggcgta ttcaacgcat tccttgattg ttgcatcact
     1501 gctccagcct gccctgataa agaacagctt tgcccaatat gtggcaccaa gatggctgca
     1561 tttttcgtta acaccaacta caaaatgtgc gcctttatca tatgtatctc taatcaaacc
     1621 atattttctt gaaccgagca ttatatcagc atctgtatat gcagctagat aaattacaat
     1681 tttagctctg gcaagtccat tagcatctat ctcttctatc gtatatgtag atgcgccatt
     1741 aaaaagacta cggctggctg caatgttacc tagactttcg ttttcctcat ttgcaaaaca
     1801 tataactgca cacgggttgc ccatttcatc gactgtagtc attcccacaa acacccatac
     1861 agcatctctc tgaaggtagt ggtatgcaaa ggatgcagta gcgttgttta atttaaatgc
     1921 gtagtaacct tttgctgtca tggtgtccac aaaggtcttg tccgctccgc gggtatcaaa
     1981 gtagttgggt acgaacaaaa tccaatccac caaaattatc tgggagtata tatcagcatc
     2041 atgttttgtt accttttcaa aggaccaggc agttccgtcg ccgagattca ggaaactcag
     2101 atatactgtt ccgaatccat tggttgccag aaaccgtccg ttatatttta tatgatagca
     2161 tccattaatt ttagggtcgt gtgttgttga aattctatct atagtaaact gttgatagga
     2221 tgctccattg tcagtccata tgtcaacatt gttaccggtt atatccaaca cttttcttgt
     2281 tgggctggac ataggctgca gtttgtatgt accatttgag tttctggtaa tcttccattg
     2341 ttgagcatat atcctgtttc cctcactgct tataactggg gtgccgttcg agtctgctcc
     2401 attttggacc tccaaatatc tgccagttat tacattcctg atgtagtata cctctcctgt
     2461 ctccaaaccc ggcacatcat tggtagctgc catcgcagca tatccgttaa agcccaggaa
     2521 gctgaacatc attgcaaaca gcaaaaccat acatattttt ttctgcatct gtacatcaac
     2581 cccttcaaga attatttaga ctattagctt gtctagtatt atatcatgaa aaaacgcaat
     2641 ttttcgggca ttgttccaaa atgtgtccca aatgttccat tcggtaattt gtatcatata
     2701 ttatggtatt tattccattt tatccctacc ctgttttgga tgcgcttcct ttatatggta
     2761 ttaccacatg gagcgtaaat acgctgttct gaaactccac attcatgaat ccaccaagct
     2821 cctgcaccgc atcccgtata atactaagac ccaaaccatg catgctcttg ttatctttga
     2881 gtgactcgaa ctggtctgtg agcgtctttt taactctgtc cgcagagttt tcaattagaa
     2941 tgactatgtt gttctcaatc ctctgaaaac ttataaatat gaatttctcc agcgtctcat
     3001 ccttgatacg catgcatgcc tcgatagcgt tgtcaatggc attgccaaga atacgacaga
     3061 cattgtacgg agatatgtat atattgtcta ttatgttgct ggtctttact acgtatctaa
     3121 tatcattctc cttggcaagc agacccttta tgttaataat tgcgtctatg gcaggattct
     3181 tcgtgtaaat catagaagca tcctctattt cagcttcaaa gtcttttatg tatttgtgca
     3241 gaagcctgat ttcattgttt tttattaagc tttcgatcat gactatatgg tttttaaagt
     3301 catgcctcat tttcctcatt tcatcgtagg agaatcccaa ttgcttgtag ttctcgtttt
     3361 ccttttctat aaggctttct aacactgtta cttttacgtt ggttgtgtag gagtcaataa
     3421 aggcaaatat gctgaagttc atgtaaagaa gacaaataat tgagatgcct aagtaaaggg
     3481 catatttatc catggaataa ataattgacc agtacactgt gttgagcatg atgacactgg
     3541 ccaatggaac agttattatt atcacccagt agtatagagg gatgtggctg aatcttctgt
     3601 tgacaatgtt agcatttgtc aagcacagcc agaacagaat tgttcgggtt gtggtaatgt
     3661 atattaattg gtaggagatg tcgtttaggt acagagggtt tatcaggaac atgttttgga
     3721 attgaaggat tataagttcc gctattagaa tataaagtat gtatgcaact attgtaagga
     3781 gcttgtctat agtgtctcct ttgaaaacta tggctagaaa tgcgtaggca agaacaaagg
     3841 aaaggataat gttgagtgca aaagtgggga tgaagtaaaa aaagacaaca cgaacaagca
     3901 gtgtcagaag ggctaatgtg tacttcaggc accttgcata ttttctttct
//
LOCUS       DAONVY010000180         3935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16161_length_3935_cov_3.615464, whole genome shotgun sequence.
ACCESSION   DAONVY010000180 DAONVY010000000
VERSION     DAONVY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16161_length_3935_cov_3.615464"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLI11_05795"
     CDS             <1..652
                     /locus_tag="PLI11_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPZ52410.1"
                     /translation="KLMEKHGIKYDRVDKIGTVVHAAKGSVYLGYIVISDKIKIDTKG
                     AVESLKREGVHNLVMLTGDSERTAVHVAKEVGIRKVYSSLLPQDKVSIVEKLLAEMGK
                     KKTLVFVGDGMNDAPVLMRADVGIAMGALGSDSAIEAADIVIMDDKPSKIPEAVQIAK
                     KTVRIVKQNITIALFVKILVLLLGAFGFANMWAAVFADVGVSIITILNAVRIYKGY"
     gene            complement(654..1307)
                     /locus_tag="PLI11_05800"
     CDS             complement(654..1307)
                     /locus_tag="PLI11_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPZ52411.1"
                     /translation="MQNRVIKGIIFDMDGVLIDSEPVMFKASVKAFKEFGIDALPSDF
                     LPYIGMDEVHFFGNVSMKYGYPYDEKIKDRAYDIYTEMIGHENAVMPNVYETVSKLKE
                     RGYKVSVASGAADIKVEANIRALGLPEDIFDYIITGSNVTKNKPDPDIFMKAAEGMGL
                     NYEECLVVEDSISGIKASKGCGMVCVGITSSLDEEKLLAAGADLTANDLIFLLNLLK"
     gene            complement(1310..3430)
                     /locus_tag="PLI11_05805"
     CDS             complement(1310..3430)
                     /locus_tag="PLI11_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52412.1"
                     /translation="MKTISLSNPDYIYRVLKIDHHLCMDINHAKKRMTDLKNKGYGGV
                     VTNVAFDDYLSNPEYMNTFSQVVRYAKEIGMRVWIYDELGYPSGTAGGLTLKANDEYE
                     AKALCATIIKVNSGDEIVIEKAKNHDFLTAYAFPINESKKESDNQELLSKPEDVYVDA
                     IIGERICLDGYLDENHTLCYIPNETSLVCYFQTKHLYEGTHAQHNVHASRRYISVASK
                     DAVREFISNTYEKYTKSVGDLYGNHIEAYFTDEPSYMAGYLNKGLYPPRVDDPYDDSI
                     PLLPVVNWDTFVRDEFFTKYGYDIYDNLHYLFAGTNNKAQKIRLDYNSLLSDLYYEAY
                     FKQIGDYCENNNVHFSGHLLLEENILHHGIYEGNVFRLIGKMGIPGIDMLTTIPEEVL
                     KWAQTPKLISSSAKWHKKQHVMTEASGHMQGAANKPIDLQMMIGSCATQFALGVDTFT
                     SYYNDNVITDEENRIFNTFVARLCNMFDGGSEEISTAIYYPIESVWVSTKGSDNQLDQ
                     REYGIGALKLEESWRGIIYELLNHQVEFDCLDANAVTESGITYSHIVVPRILSIPDFL
                     YDRLDNLAKDGVTIIFHETSEMVKEKIEKLSTESNVMIVDKIEDIARTIKDNTSKDIE
                     IDSNRGVVVKSKNNVKIGFDRVVFFVNTNKDKTNVTVDINKECELKIYDPLENTFVNY
                     DSQTVNFLLGGYQSKILCIDWKGN"
     gene            complement(3436..>3935)
                     /locus_tag="PLI11_05810"
     CDS             complement(3436..>3935)
                     /locus_tag="PLI11_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977160.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPZ52413.1"
                     /translation="DIIGVKFKNNEVYVPEVLVAARAMNMGMEILKPHLAEAGVKAKG
                     KVVIGTVAGDQHDIGKNLVKMMMEGKGLEVIDLGVDVPAETFVQAAIDNNAEIIACSA
                     LLTTTMVSIKSIVEKAKEMGIRDKVKIMIGGAPVTQDFCNEVGADCYRPDAASAAEAA
                     VELLG"
BASE COUNT         1120 a          826 c          677 g         1312 t
ORIGIN      
        1 caagttgatg gaaaagcacg gcataaaata cgacagagtg gataagatcg gaactgttgt
       61 gcatgcggca aaaggttctg tttaccttgg atatattgta atatcagaca aaataaaaat
      121 agataccaaa ggtgcagttg aaagtctgaa aagagaaggg gtacataatc tagtcatgtt
      181 gactggtgac tctgaaagga ccgcagtgca tgtggcaaag gaagtaggaa ttaggaaggt
      241 atattcttct cttctgcctc aagacaaagt gagtattgtt gaaaaacttc ttgcagagat
      301 ggggaaaaag aaaacccttg tttttgtagg agatggtatg aatgatgcgc ctgttttgat
      361 gagagctgat gttggtatcg ccatgggagc gcttggctct gacagcgcta ttgaagctgc
      421 tgacatagtt attatggacg acaagccgtc caaaattcca gaagctgttc aaatagcaaa
      481 aaagacagta agaattgtaa aacagaacat aacgattgcc ctgtttgtaa agattcttgt
      541 tcttttgcta ggtgcttttg gttttgccaa tatgtgggct gcagtatttg cagatgttgg
      601 cgtttcaata ataacaattc tgaatgcggt aaggatatac aaaggatatt gatctattta
      661 agcaaattca ataagaaaat cagatcattg gctgtgaggt ctgcacccgc agccaataat
      721 ttttcctcat caagagaact tgtaatgcca acacatacca ttccgcatcc cttggaagcc
      781 tttatgccgc ttattgagtc ttccacaacc aaacattctt cataattcaa gcccattcct
      841 tctgcagctt tcataaagat gtcaggatcc ggcttgtttt ttgttacatt actaccggtg
      901 attatgtaat caaaaatatc ttcaggcaat cccagtgctc ttatatttgc ctcaactttt
      961 atatcagcag caccagatgc aactgaaact ttgtatcctc tttcttttag tttgctgact
     1021 gtttcataaa catttggcat gacagcattt tcatgcccta tcatttccgt ataaatgtcg
     1081 tatgccctat cttttatttt ctcatcataa ggataaccat acttcatgga aacatttcca
     1141 aagaaatgta cctcatccat cccgatatat ggcaggaagt cgcttggtaa tgcgtctatc
     1201 ccaaattcct tgaaagcttt aacagacgcc ttgaacataa caggttctga atcaatcaaa
     1261 accccgtcca tatcgaagat tattcccttt attactctat tttgcaattc taattccctt
     1321 tccagtcgat acaaagtatt ttagattgat atcctcccaa taagaagtta acggtctgac
     1381 tgtcataatt aacaaatgtg ttttccaaag gatcataaat tttcaattca cattccttat
     1441 ttatatccac agttacatta gtcttatcct tattggtgtt tacaaaaaaa acaaccctgt
     1501 caaacccaat cttgacattg ttcttggatt ttactacaac tcctctgttt gagtcaatct
     1561 cgatgtcttt acttgtattg tcttttatag tccttgcaat atcttcaatt ttatccacaa
     1621 tcattacatt actttctgtg gataactttt ctattttctc ttttaccatt tcacttgttt
     1681 catgaaatat tattgttact ccatcttttg ctagattatc aagcctgtca tatagaaaat
     1741 ctggaatgct tagtattctt ggaacgacaa tatgtgaata tgtgatgcct gattctgtaa
     1801 cagcatttgc atccaaacaa tcaaactcaa cctgatggtt tagaagctca taaattatcc
     1861 ctctccagct ctcttccagc tttaatgctc caataccgta ttccctttgg tcaagctgat
     1921 tgtctgagcc tttcgttgat acccaaacac tttcaatggg atagtatatg gctgtggaaa
     1981 tctcttcact tccaccatcg aacatattgc aaagtcttgc tacaaaggtg ttgaaaatac
     2041 ggttctcttc atcagtaatt acattgtcgt tgtaatatga tgtaaatgtg tccacaccta
     2101 aagcaaactg ggttgcgcaa gagcctatca tcatttgcag gtctataggc ttattcgctg
     2161 caccttgcat atgtccggaa gcttctgtca taacatgttg tttcttatgc cattttgcag
     2221 aagaacttat gagttttgga gtttgtgccc atttcaacac ctcttcaggt attgtggtca
     2281 gcatatctat tcccggtatg cccattttcc caatcagcct aaacacattt ccttcatata
     2341 ttccatgatg cagtatgttt tcttcaagaa gcaaatgtcc tgaaaaatgt acattgttgt
     2401 tttcacaata gtcgcctatt tgcttgaaat atgcttcata gtacaaatct gacagtaaag
     2461 aattgtaatc caatcgaatc ttttgggctt tattatttgt tcctgcaaac aaatagtgaa
     2521 gattatcata aatgtcatat ccatatttgg taaaaaactc atctctcaca aaagtatccc
     2581 aattaacaac aggaagtaga ggtatggaat catcatatgg atcatccacc cggggtggat
     2641 aaagcccttt attcaaataa cctgccatat aggaaggttc atcggtaaag taggcttcaa
     2701 tatgattgcc gtacaaatcc cctactgatt ttgtatactt ttcatatgtg tttgaaataa
     2761 attctctgac tgcatcttta gaagcaacac taatgtatct tctggatgca tgcacattgt
     2821 gctgtgcatg agttccttcg tatagatgtt tcgtttggaa gtaacagaca agggatgttt
     2881 catttggtat atagcagaga gtatgattct cgtccaaata tccgtcaaga cagatcctct
     2941 cccctattat tgcatccaca tagacatctt caggcttgga aagcagctct tgattatccg
     3001 attccttttt gctttcattg ataggaaaag cataagctgt gagaaaatca tgattcttag
     3061 ccttttcaat gacaatctca tctcctgaat taacctttat gatagtagca catagtgctt
     3121 tcgcttcata ttcatcattt gcctttaatg taagtcctcc tgcagtaccg ctgggatagc
     3181 cgagttcatc gtatatccag actctcatac ctatttcctt tgcatacctc acaacttggc
     3241 tgaaggtatt catgtattca ggatttgaca aataatcgtc aaaggctacg tttgtgacga
     3301 cacctccgta gcctttgttt ttcaaatctg tcattctctt ttttgcatga ttaatatcca
     3361 tgcagagatg gtgatctatt tttaatactc tgtatatgta atcaggattg cttaatgaga
     3421 ttgttttcat actttttagc ctaacaattc aacagccgct tcagctgccg atgcagcatc
     3481 cggacgatag cagtcagctc ctacttcatt gcagaagtcc tgagtaacag gagctccacc
     3541 gatcatgatt ttcaccttgt ctcttatgcc catttctttt gctttttcaa caatggactt
     3601 aatgctgacc atggttgtag taagcagagc tgaacaagct atgatttctg cattgttgtc
     3661 aattgcagcc tgaacaaacg tctccgcagg aacatcgaca cccagatcaa taacttcaag
     3721 gccttttcct tccatcatca tcttgacaag attcttgcct atatcgtgct ggtcaccggc
     3781 aacagtgcct attacgactt ttcctttagc ctttacgcct gcttctgcca agtgtggctt
     3841 taaaatttcc attcccatat tcattgctct agctgcaacc agtacctctg gaacgtatac
     3901 ctcgttattc ttaaatttaa caccaattat atcca
//
LOCUS       DAONVY010000181         3927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16197_length_3927_cov_3.341684, whole genome shotgun sequence.
ACCESSION   DAONVY010000181 DAONVY010000000
VERSION     DAONVY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16197_length_3927_cov_3.341684"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3244)
                     /locus_tag="PLI11_05815"
     CDS             complement(<1..3244)
                     /locus_tag="PLI11_05815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPZ52414.1"
                     /translation="MVQDVDVNVKDVQYGRTTRKSYSKINEILEMPNLIAVQKDSYQH
                     FKDVGLKEVFRDISPITDYSGNLVLEFVDYVIEEDKAPYTIEECKERDATYAAPLRVK
                     ARILNKLTGIVKETDTIYFGEFPLMTENGTFIMNGAERVIVSQLVRSPGVYFDRTYDK
                     TKGKYITTSTLIPNRGAWLEFEIDHSDVIYVRIDRNRKMPATQLLRAIGYGTNDQIIE
                     LLSVNKEEATADGFDDSDDGWIKDERVMNSLDKDSAEDNGEDRALIEIYKRLRPGEPA
                     LVDNARTLLESMFFDPRRYDLARVGRYKVNKKLSLAARITGKAAAGDIVDPQTGEILV
                     EAGKLIDEKTAWDIQNSGLNEVNVMTPYRPVKIIGNKMVDAKAYLDFDPAEVGINEHV
                     YFDVLMEILESTDDENELKVQLATRINELIPKHITREDIVATINYMLCLQYDLGQIDD
                     IDHLGNRRVRAVGELLQNQFRIGLSRMERVVKERMAMQDMDAVTPQALINTRPVTAAI
                     KEFFGSSQLSQFMDQTNPLAELANKRRLSALGPGGLNRDRATFEVRDVHYSHYGRMCP
                     IETPEGPNIGLIGSLSTYARINEYGFIEAPYRKFNKETGKVTDEIHYLMADEEDNYVI
                     AQANEPLDEEGRFVNKKVVCRFRDEFIEVESDKVDYMDVSPKQVVSVAAAMIPFLEND
                     DASRALMGSNMQRQAVPLLRSEAPIVATGIEYKAARDSGAVVIAKADGEVVDVTSNYI
                     TIRRDNGKIDTYNMLKFMRSNQGTCINQKPVVRIGERVKKDEVIADGPSTDNGEIALG
                     KNVLIGFMTWEGYNYEDAVLISERLVKEDVYTSIHIEEYECEARDTKLGPEEITNEIP
                     NVGEDALRYLNHEGIIHIGAEVQAGDILVGKVTPKGETELTAEERLLRAIFGEKSRDV
                     RDTSLRVPHGESGIVVDVKVFTREHDDDLAPGVNKLVRVYVAQKRKLSVGDKMAGRHG
                     NKGVISRILPEEDMPFLPDGTPLDVVLNPLGVPSRMNLGQVLEVHLGYAARALGWKVA
                     SPVFDGATEDDIMNTLSKAGLPEHGKTILRDGRTGEEFDNPVTVGYMYYMK"
     gene            complement(3543..3764)
                     /locus_tag="PLI11_05820"
     CDS             complement(3543..3764)
                     /locus_tag="PLI11_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52415.1"
                     /translation="MSKTVSILLVLFLFVNMMPVAANADTSPEPEDNGDGKGFNWTLA
                     FILSSIASFIIATIIAVKQANKRFPDEKS"
BASE COUNT          996 a          960 c          727 g         1244 t
ORIGIN      
        1 gcttcatgta gtacatgtat ccgactgtta cggggttgtc aaactcttct cccgttctgc
       61 cgtctctgag tatggtcttt ccgtgctcgg ggagacccgc cttgctcaga gtattcatga
      121 tgtcatcctc ggtagcaccg tcaaatactg gagatgcaac cttccagccc agcgctcttg
      181 ctgcatatcc caggtgaacc tccagaacct gacccaggtt cattcgtgaa ggaacgccca
      241 gaggattcaa tacaacatcc aatggagtgc cgtccggcag gaatggcata tcctcttcag
      301 gaagtattct ggatataacg cccttgttac cgtgacgtcc cgccatcttg tcgccaactg
      361 aaagctttct cttttgtgca acatatactc taacaagctt gtttacgcca ggtgcaagat
      421 catcgtcatg ctcccttgtg aacaccttga catccacaac gatacctgac tctccatgag
      481 gcactctgag ggaagtgtct cttacatccc ttgacttctc accgaagatt gcacgcaaga
      541 gtctttcttc agctgtaagt tcggtctccc cttttggagt aacctttcct actagaatgt
      601 ctcctgcctg aacttcagca ccgatgtgaa tgataccttc atggttgaga tatctcaaag
      661 cgtcctcgcc tacgttgggt atttcgtttg tgatctcctc aggaccaagc ttggtatccc
      721 tggcttcgca ttcatactct tctatgtgta ttgatgtgta tacgtcttct tttaccaatc
      781 tctcgcttat aaggacggcg tcctcataat tgtagccttc ccatgtcata aaccctataa
      841 gcacgttctt tccaagggca atctcgccat tgtcggtgga tggaccgtca gcaattactt
      901 cgtccttctt aaccctttcg ccaatcctta caacaggctt ctgatttatg caggttccct
      961 ggttggaacg catgaacttc agcatattgt atgtgtcaat cttgccattg tctcttctta
     1021 tggttatata gttgctggta acgtcaacaa cctcgccgtc tgccttggca atgacaacgg
     1081 ctccggagtc ccttgcagcc ttgtattcaa taccagtagc aacaatgggt gcctcactgc
     1141 ggagaagcgg cactgcctgc ctctgcatgt tggagcccat gagtgcacgg gatgcgtcgt
     1201 cgttctcaag gaatggaatc attgctgctg ctacggaaac aacctgtttt ggcgaaacgt
     1261 ccatgtagtc aaccttgtca gattcaacct ctataaactc atccctgaaa cggcagacaa
     1321 ccttcttgtt tacgaatctg ccttcttcgt ccagaggttc gtttgcttgt gcaattacat
     1381 agttgtcctc ttcgtcagcc atcaggtaat gtatctcgtc tgttaccttg cctgtttcct
     1441 tgttaaattt acgataaggg gcctcaataa atccgtactc gttgattctc gcgtatgtac
     1501 tcagcgagcc aataagaccg atgtttggac cttcaggtgt ctcaattgga cacatcctgc
     1561 catagtgcga gtagtggacg tcacgaacct caaaggttgc cctgtcccta ttcaaaccac
     1621 caggtcccaa tgcactgagt cttcttttgt tcgcaagttc agcaagcggg tttgtctgat
     1681 ccatgaactg cgacagctgg ctggagccaa agaactcctt gatagctgca gttacaggtc
     1741 ttgtgttgat gagagcctgt ggagtaacgg catccatgtc ctgcattgcc atacgctcct
     1801 ttacaacacg ttccattctg gacaaaccaa ttctaaactg gttctgtaaa agctctccta
     1861 ccgcccttac tcttctgttg cctaaatgat ctatgtcatc tatttgtccc aaatcatact
     1921 gcaagcacag catgtagtta attgtggcta ctatgtcttc tcttgtaata tgttttggaa
     1981 tcagctcgtt aattcgtgtt gcaagttgaa ccttgagctc attctcatcg tcggttgatt
     2041 caagtatttc cataagcacg tcaaaataga catgctcgtt tattcccact tcagcaggat
     2101 caaaatcgag atatgccttt gcatcaacca tcttgttgcc gatgatcttt acaggtctgt
     2161 atggagtcat tacattaact tcattaagac cactgttttg tatatcccaa gctgtttttt
     2221 cgtcaatcag cttgccagcc tcaacaagaa tctctcctgt ttgaggatct acaatatcac
     2281 ctgccgctgc ctttccagtt attcttgcag caagtgaaag ctttttgtta actttgtatc
     2341 ttccaaccct tgcaaggtca tatcttcttg gatcgaaaaa catgctttca agcagtgtcc
     2401 ttgcattgtc aaccagagcg ggctcgcctg gacggagtct cttgtatatc tcaataaggg
     2461 ctctgtcttc tccattgtcc tccgcagaat ccttgtcaag actattcatt acacgctcgt
     2521 ctttaatcca gccgtcatcg gaatcgtcaa agccgtcagc tgttgcttct tccttgttaa
     2581 cggacaaaag ctctatgatc tggtcattgg ttccgtaacc aatagccctc aagagctggg
     2641 ttgcaggcat ttttctattc ctgtcaatcc taacgtatat gacatcgcta tgatcaattt
     2701 cgaactcaag ccatgctcct ctgtttggta tgagggttga tgttgtgatg tacttaccct
     2761 tggtcttgtc gtatgttctg tcaaaataca cgcctggaga ccttaccagc tggctaacta
     2821 ttaccctttc ggcgccattc ataataaatg taccgttttc cgtcatcagc ggaaactcac
     2881 caaagtatat agtgtcggtt tcttttacaa tacctgtcag tttgttcaga atacgggctt
     2941 ttaccctgag aggagccgca taagttgcgt ctctttcctt gcactcctct attgtataag
     3001 gagccttgtc ttcctctata acgtaatcga caaattcgag caccaaatta ccggaatagt
     3061 ccgttatagg tgatatgtct ctaaaaactt ctttaagtcc aacatcctta aaatgctgat
     3121 atgaatcttt ctgaacagct ataaggttgg gcatctcaag aatttcgtta atcttggaat
     3181 aactctttct tgtagttcgg ccgtactgta catccttaac attaacatca acatcttgca
     3241 ccatactgat tgttcacccc aaattcaatt ttttgctacc ataccttatt attccacatt
     3301 attccttgtg ttaaacaagc tatttggttg atgttcatac aaaaatatgt cagagcaaat
     3361 attttttatt gccatctata tttttgtgtg ctataatatc cttgtaactt caccatattt
     3421 aacttaaaaa cataataaag cctaaaaaga gattgcagcg cagtttatta ttctatcact
     3481 taaacaataa caaagtcaac aggtaataat taaaaacctg ctgattaatt gttaacattc
     3541 tattaactct tttcatctgg gaaccgttta ttagcttgtt ttactgcaat gatagttgct
     3601 atgataaaac tagctatgct ggacaaaata aatgccaatg tccagttgaa gcctttgcca
     3661 tctccattat cctctggttc aggtgaagta tcggcatttg ccgccactgg catcatatta
     3721 acgaataaaa aaagaaccaa caatattgaa actgttttag acatatagcc cccgatatta
     3781 taatattgga tactaaccta taatatcaca attaaaaaaa atatacaaca taaatttttc
     3841 tataaaaaaa agaggtggat ttccacctca attcgatagt ttgacacttg ctcacatatc
     3901 cgattttcag aaaagctcat aatatga
//
LOCUS       DAONVY010000182         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16263_length_3914_cov_3.827676, whole genome shotgun sequence.
ACCESSION   DAONVY010000182 DAONVY010000000
VERSION     DAONVY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16263_length_3914_cov_3.827676"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLI11_05825"
     CDS             <1..1049
                     /locus_tag="PLI11_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPZ52416.1"
                     /translation="TDIFHPVTKKLLSTIYIDDKPRLVTLDDEFEKIMANVREDFDTN
                     TFPVTVSFDLNYMVLMHRDHRDLGTYYLYNTKTRSSKVLASPQIDAESLGITLPISFK
                     ASDGETIYGYLTLPKGKKPDNLPLMVNVHSGPSSRFGWTPSVETILASDAGYAVLSVN
                     SRISTGYGNEYIDFAVKDLLLPQKDIREAVDWAIETGIADSKNISIMGHSYGGFSALY
                     QAGVYPSMYKSVIAMMGVWDWTDLGNELAEEEELPVWYKRFAPVPKSELATVLSPSTY
                     VDRIKMPIFIVYSGKDEVVYPSQNIRAILELSANGNTPESLYLTEEGHFPENISSRKM
                     IISHLSEFLNKNIN"
     gene            1074..2390
                     /locus_tag="PLI11_05830"
     CDS             1074..2390
                     /locus_tag="PLI11_05830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPZ52417.1"
                     /translation="MQKRLKNFKFLLLSFVVGTIVLIYIVVYLPVHSELAVMFNKTFW
                     QSSQDYGSMINHTLEDCIRDAAYLSSNHTMLTQIEDYEANNISFEALKEQLQQVYGNF
                     AIKIDRLTSAARYLGNYVVCSYNDGTSYDISPDNDFLFNGNNTYFKVYSPIYSNNKQI
                     GFDIIIFDITGMLRTRTHVSKITSPEAVKKIVDNKTIYQTSNKTLYYDRNGIIYTEEI
                     TGMEYYLYITADNETLYKPLRSIAIYFFIGLAASLSLIALLTNFLVVKRAGRMIAISE
                     KRGEMYKKDSMLDPLSGAYSRRFFEEWIKKESISNSKENFHYTMVLLDVDRFKQINDS
                     CGHLSGDEAIKIVGSALFSCVRTTDYVIRYGGDEFMLLLNECSESQAEDIMYRVCDKL
                     RKIDTVPFEISISYGIQEIKSRNDILKAMNQADNKMYEAKKTKNIS"
     gene            complement(2431..2667)
                     /gene="tlp"
                     /locus_tag="PLI11_05835"
     CDS             complement(2431..2667)
                     /gene="tlp"
                     /locus_tag="PLI11_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein Tlp"
                     /protein_id="HPZ52418.1"
                     /translation="MSKKKPKPDDRSDNVEKIQYNINRTIQNMELADELIAKSSDENL
                     KQALIEKNERRRHALEGMREEIRDEALARERGET"
     gene            complement(2779..3072)
                     /locus_tag="PLI11_05840"
     CDS             complement(2779..3072)
                     /locus_tag="PLI11_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52419.1"
                     /translation="MKRKIVLIVLMSLILMVMLAGCIPGDGKHNEENKAGFFWGVWHG
                     WIAPISLIGGIFNKDLRVYEVANTGWWYDFGFYIAIISGFGGISLTRRKIKRD"
     gene            complement(3095..>3914)
                     /locus_tag="PLI11_05845"
     CDS             complement(3095..>3914)
                     /locus_tag="PLI11_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52420.1"
                     /translation="NVKIMPPTHVTHAARLLNYLCHDLTEKKKKPNIKQIKLHQQARI
                     GESIQMYKDIESEGSNYPYEKEKELITKVKLGNIADAKGILNDLLGYVFFSEGNNLDA
                     IKTRAVELSSLLSRAAIEGGAATDRILKMNNYFLRTIQQINNLEDLCYRLQEVVIEFT
                     EAMFHQIPSKNSEVIKNAIKYITKNFSRQITLEEVASVVHLSSSYFSTLFKECTGSTF
                     KEYLNMIRVEESKRLLSNTDYSIIDIAIATGFESQSYFTKIFKRYTGYTPKQFR"
BASE COUNT         1196 a          816 c          731 g         1171 t
ORIGIN      
        1 ccaccgacat atttcaccct gtgacaaaga aacttctttc aaccatatat attgatgaca
       61 agccccgtct ggtaaccctg gacgatgaat ttgaaaagat aatggcaaat gtaagggaag
      121 attttgatac caatactttt ccagtaacgg tctcgttcga ccttaactac atggtgctga
      181 tgcacagaga ccaccgtgac cttggaacgt attaccttta caacacaaaa accaggtcat
      241 ccaaggtttt ggcttctcct caaatagatg ccgaatccct gggaataacc cttccaatat
      301 ccttcaaggc atcagacgga gagacaattt atggctatct gactttacca aagggaaaga
      361 agcctgataa ccttcccttg atggtaaatg tgcacagcgg tccctcatca aggtttggct
      421 ggacaccttc ggtggaaacc atcttggcat cagacgcagg ttacgcagtt ctgtcagtaa
      481 acagcaggat atccaccggt tatggaaatg agtacattga ctttgcagta aaagaccttc
      541 ttttacctca gaaagacata cgagaggctg tggactgggc aatagaaaca ggaattgcag
      601 acagtaaaaa tataagcata atggggcatt cctacggagg attctctgcc ctttatcagg
      661 caggagtgta tcccagcatg tacaagtctg taattgccat gatgggtgtc tgggattgga
      721 cggacttagg taatgagctg gccgaagaag aagaacttcc tgtttggtac aagagatttg
      781 cacccgttcc taaatccgaa ctagccaccg tattgtcacc gtcaacttat gtagacagaa
      841 tcaagatgcc catatttata gtatactcag gcaaggatga ggttgtttat ccatcccaga
      901 acataagggc gatacttgaa ctttcagcaa acggcaatac tcctgaaagc ctttatctta
      961 ctgaagaagg tcattttccg gagaatataa gcagtcgcaa gatgataata agtcacttga
     1021 gtgagttttt gaataaaaat attaattgac gcaaggggta cgatagtgta gaaatgcaaa
     1081 aaagactaaa aaacttcaaa ttcctgctat taagttttgt agtaggtacg atagttctca
     1141 tctacatagt tgtatatctg cctgttcaca gtgaactggc cgtcatgttt aataaaacct
     1201 tttggcaatc ttcgcaggat tatggctcaa tgataaacca taccctggaa gactgcataa
     1261 gggatgctgc gtatttgtcc agcaaccaca ccatgcttac tcagatagaa gactacgaag
     1321 caaataacat atccttcgaa gcgttaaaag aacaactaca acaggtatat ggaaactttg
     1381 ctattaaaat tgacagactg acaagtgccg caaggtatct tggcaattat gttgtctgct
     1441 catacaatga cgggaccagt tatgatattt cgccagacaa tgattttctt tttaatggca
     1501 acaacaccta cttcaaggta tattctccaa tttactcaaa caacaagcaa attggttttg
     1561 atattataat atttgacata acaggtatgt taagaaccag aacacatgta tcaaaaataa
     1621 caagccctga agcagtgaag aaaattgttg acaacaaaac aatatatcaa acaagtaaca
     1681 aaacattata ttatgacaga aacggaatca tatacacaga agaaataact ggtatggagt
     1741 attacctcta tataacagcc gacaacgaga ctctttacaa gcccttaaga agtattgcca
     1801 tatacttttt cattggcctt gctgcatcct tgtcgttaat tgccctgctt acaaacttcc
     1861 ttgttgtaaa aagggcagga agaatgattg ccattagtga aaaaagagga gaaatgtaca
     1921 aaaaagattc catgctggat ccactttcag gggcgtattc aagacgattc tttgaggagt
     1981 ggattaagaa ggagtcaata agcaactcta aggaaaattt ccactacaca atggtgctgc
     2041 ttgacgtgga ccgtttcaag cagatcaacg actcctgcgg acatttaagt ggagacgagg
     2101 caataaaaat tgtgggttcg gctttgtttt cctgcgtccg tactacggat tatgttattc
     2161 gctatggagg agacgagttt atgcttcttt tgaatgaatg cagcgaatcc caagcagagg
     2221 acatcatgta cagagtttgt gacaaattaa gaaaaattga tactgttccg tttgaaatat
     2281 cgatttccta tgggattcag gaaatcaaat caagaaatga catattaaag gccatgaatc
     2341 aggcagataa taaaatgtac gaagcgaaaa agacaaaaaa catctcatag aaaaaaccat
     2401 atctccaaga gatacggttt tttatttata tcaagtttcg cctctttcac gagccagagc
     2461 ctcatccctg atttcctccc tcatgccttc aagggcatga cgccttcttt cattcttctc
     2521 tataagtgcc tgtttcagat tctcatcgga ggattttgca atcaactcat cagcaagctc
     2581 catgttctgt atggttctgt taatgttgta ctggattttt tccacattat cgcttctgtc
     2641 atccggttta ggcttctttt ttgacatttc atcccctcct tttacttgtt cttttaatat
     2701 tttttcaaga tgaatagaaa aatatttgtg aaagattatg ttaataaaaa accaggaggc
     2761 aatactcctg gttctccatt aatccctttt tattttcctt cttgtcaatg atattcctcc
     2821 aaatccggat ataattgcta tataaaagcc gaagtcatac caccagcctg tatttgccac
     2881 ctcatacacc cttaaatcct tgttgaatat accaccaatc agagatattg gcgcaatcca
     2941 gccgtgccat actccccaga aaaagcctgc cttgttttcc tcattatgct tgccatctcc
     3001 aggaatgcag cctgccagca tcaccattag aatcaaggac atcaaaacaa tcaacactat
     3061 ctttcttttc aaattaattc ccctttacca tatgttatct aaactgcttg ggcgtataac
     3121 ccgtatatct cttaaaaatt ttggtaaagt agctttggct ctcaaagcct gtagctattg
     3181 caatatctat aattgagtaa tcggtgtttg aaagaagtct tttgctttct tccactctta
     3241 tcatattcag atactccttg aaagtagacc cggtacattc cttgaacagt gttgagaaat
     3301 aggaggaact caagtgtaca actgaagcaa cctcctcaag ggtaatctgt ctcgaaaagt
     3361 tctttgtaat atatttgatt gcattcttga taacctcgct gttctttgaa ggaatctgat
     3421 ggaacatggc ttctgtaaac tcaataacca cttcctgcag tctgtagcac aaatcttcca
     3481 gattgtttat ctgctgtatt gttctaagga aataattatt catttttaga atcctgtccg
     3541 tggcggctcc tccttcaatt gcagcacggg ataaaagcga gctaagttca acagctctgg
     3601 tctttatggc gtcaagattg tttccttctg agaaaaatac atatcccaac aggtcattca
     3661 aaataccctt cgcatccgct atgttgccca gttttacctt tgttattagt tccttttcct
     3721 tttcataagg atagtttgaa ccttcactct cgatatcctt atacatctgt atggattcgc
     3781 ctattctcgc ctgctggtgt aactttatct gctttatatt tggctttttc ttcttttccg
     3841 taaggtcatg gcacaaataa ttaaggagcc ttgcagcatg ggtgacatgc gtaggcggca
     3901 ttattttcac attt
//
LOCUS       DAONVY010000183         3908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16302_length_3908_cov_3.404360, whole genome shotgun sequence.
ACCESSION   DAONVY010000183 DAONVY010000000
VERSION     DAONVY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16302_length_3908_cov_3.404360"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLI11_05850"
     CDS             complement(<1..298)
                     /locus_tag="PLI11_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52421.1"
                     /translation="MTICILMGPYPTDENPINAFTDMLVTEMANQGVNCVVIAPQSRT
                     EHFFWKTKPAPLVREKTAPNGNKITIYSPVYNSWSAKSILGFNTARLTQRSYERV"
     gene            421..1476
                     /locus_tag="PLI11_05855"
     CDS             421..1476
                     /locus_tag="PLI11_05855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPZ52422.1"
                     /translation="MLFSIITPVYNEEKYITQCIESVLNQTYANFELILVDDGSTDNC
                     VQIMDDYAKKDARVKVHHQENQGPIAAKDKAISLATGEYCVFLDSDDYFEPILLETVY
                     EAIKRTNCDLCMYKWYNVDEKGKFTIEGVFEHNTFFDKDNMHEFYKKLITVNELNSVW
                     IKAIRADILKSIKRDPNFLWIRREEDLYISLQIFTRAESILYLDVPLYNYRTTNRESM
                     TKKFDPTIFKSSTAVRKEVFRHLGIWGIDDEQSKTTFYNWFVKNAFRLMKGSFMLGIS
                     RKEKLVLLREMVQDDFFNDALRKVDKSYYSKLDRIEIFLFKHKLVVLLYLFNTMIFNR
                     YAAKPLIERKEMRKRNA"
     gene            1469..3061
                     /gene="cls"
                     /locus_tag="PLI11_05860"
     CDS             1469..3061
                     /gene="cls"
                     /locus_tag="PLI11_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPZ52423.1"
                     /translation="MHKKTELDKLHTMDLPKRKKASLVRIIAALTVFLVQIVFLIYAL
                     YSLNVFALSAYIVFQGISAVTAMRIVSNQSNPSYKIAWIVLILSIPFLGLLFYIMWGR
                     GRNPNNTKRRIAQLSTMTVPLYKRFKDVEESILKEYPSQSRVTKFLLESGFPVYNNTE
                     TVYFSEGDKFYEAMLEEVKKAEKFIFLEFFIISKGHMWDTLLEVLKEKVSKGVEVRLV
                     YDDFGCISTLPKKYYLELRKHGIKAVVFNPIKPLINNFYMNYRNHQKICVIDGNTAFT
                     GGINIADEYINRVEAYGHWKDSGIMLKGEGVWSLTVMFLQIWSYAAAKSYPADCDYTK
                     FKPTYIHNSGIIHKGYVQPFSDGPLNNPNNPAEFSYMQMINTARRYIYITTPYLILDN
                     EMVTSLRLAAQSGIDVRIITPGIPDKKYVFAVTRTFYGVLLETGVKIYEYVPGFIHSK
                     NVVCDDETAIVGTINMDFRSFYLHFENGVWLCGTDSVFDIKKDFLETLDMCREITYEE
                     WKKRPLYYRFIQSILHVFAPML"
     gene            3168..>3908
                     /locus_tag="PLI11_05865"
     CDS             3168..>3908
                     /locus_tag="PLI11_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPZ52424.1"
                     /translation="MDFAYLIVGISKNEEKDLKELYDKTHHTVYGLALSLVRDRVLAR
                     DITIETYRRVKQFANTYDTDINAEYWLLDITKNLSINALHDKDIHAALKEGKLDNVSK
                     AVRDTVSSLKNDRGSIIVLKAMSGLSMKEIANLLWYYHASARNEYKRGLKELRKKDNV
                     KRSASDILEYIRRDITSSAPDVYDQILSDKQTRVSFVSHEVLNLSEDEITFTDKDAEL
                     EYIRRLAKSGKSAKRARLGGIAAMVLVCV"
BASE COUNT         1269 a          709 c          808 g         1122 t
ORIGIN      
        1 caactctttc ataacttctt tgcgttagtc ttgctgtatt gaaccccaat atgctctttg
       61 ccgaccagga gttgtaaact ggtgaatata tcgttatctt gttgccattg ggtgctgttt
      121 tctcccttac cagtggagca ggcttggtct tccaaaaaaa atgttccgtc ctgctctgtg
      181 gcgcaataac cacgcaatta acaccctggt ttgccatttc ggtaaccaac atgtcagtaa
      241 acgcattaat tggattttca tcagttggat aaggtcccat caagatgcat atggtcaaaa
      301 caatcactcc ttgacatttc taccattaat cgtacccagt tacacgtaat tagtcaagct
      361 tgaattattt acttgtattg catataataa cagtgttcat tgagagaggt ggattttatc
      421 gtgcttttta gtataattac tcccgtttac aacgaagaaa aatatataac acagtgcata
      481 gaatcggtat tgaaccagac ctatgccaac tttgagctga tacttgtaga tgacggctcc
      541 acagacaact gtgtacagat aatggatgat tacgcaaaaa aggatgcgcg tgtcaaggtc
      601 caccatcagg aaaaccaagg ccccattgct gcaaaagaca aggcaatttc acttgcaaca
      661 ggagaatatt gtgttttttt ggactcagac gactactttg agcccatatt gctcgaaaca
      721 gtgtatgaag ccataaagag aacaaactgc gatttgtgca tgtacaaatg gtacaatgtc
      781 gatgaaaaag gaaaatttac aatagaaggc gtgtttgagc acaacacctt ctttgacaag
      841 gataacatgc acgaattcta caaaaaactc ataacagtaa acgagctcaa ttccgtttgg
      901 ataaaggcaa taagggcaga cattttaaaa agcataaaaa gggatccaaa cttcctatgg
      961 ataagaagag aagaagattt atacatttcc cttcagatat tcacaagagc tgaaagtatt
     1021 ctatatcttg atgtgcctct ttacaactac agaaccacaa accgtgaaag tatgaccaaa
     1081 aagtttgatc caacaatttt caaaagcagc actgcggtta gaaaagaagt tttcaggcat
     1141 ctgggcatat ggggtataga cgatgagcaa tccaaaacaa ccttttacaa ctggtttgta
     1201 aaaaacgcct tcaggcttat gaagggctcg tttatgcttg gaatatcaag aaaagaaaag
     1261 cttgttcttt tgagggaaat ggttcaagac gatttcttta atgatgcttt aagaaaagtc
     1321 gacaaatcat attactccaa attggacagg atagaaatat ttttattcaa acataagtta
     1381 gtagttttac tttacctttt caatacaatg atttttaaca gatatgcggc aaaaccattg
     1441 attgaaagaa aggaaatgag gaaaagaaat gcataaaaag actgagttgg acaaattgca
     1501 cacaatggac cttccaaaga gaaaaaaagc aagtctggta agaataattg ctgctttaac
     1561 agtgtttctt gtgcagattg tcttccttat atatgccctt tattcattaa atgtttttgc
     1621 cttgtccgca tatatcgtat ttcaaggaat aagtgccgtt accgctatga gaattgtatc
     1681 aaaccagtcc aatccgtcat acaagattgc ctggattgta ttgatactat ccatcccttt
     1741 tttgggactt cttttctata tcatgtgggg aagaggaaga aaccccaata atacaaaaag
     1801 aagaattgcg caacttagta caatgacggt acctttgtac aaaagattca aggacgtcga
     1861 ggaatccatc ctgaaggagt atccttccca aagcagggtc acaaagttct tgcttgaatc
     1921 cggatttcct gtatacaaca atacggaaac cgtatatttc tctgaggggg acaagtttta
     1981 tgaagcaatg ctggaagaag tcaagaaggc agaaaaattc atttttcttg agttttttat
     2041 aatctcaaaa ggacacatgt gggacactct tcttgaggtt ctcaaagaga aggtgtccaa
     2101 gggtgtggag gtaagacttg tttacgacga ctttggctgc atatccacgc taccaaaaaa
     2161 gtattacctt gaacttcgca agcatggcat aaaggctgtt gtttttaatc cgataaagcc
     2221 tttaataaac aacttttaca tgaattacag gaaccatcag aagatttgtg taattgatgg
     2281 aaacaccgct tttaccggtg ggataaatat tgcggatgag tatattaaca gggttgaagc
     2341 atatggccac tggaaagact caggaattat gttaaaaggc gaaggtgtct ggagtcttac
     2401 ggtcatgttt ctgcagatat ggagctatgc tgcagcaaaa tcatatccag cggattgtga
     2461 ttatacaaaa tttaaaccta cgtatataca taattcaggt ataattcata aaggctatgt
     2521 acagcctttt tctgacgggc cacttaacaa cccaaacaat ccggcagaat tttcatacat
     2581 gcagatgata aacactgcaa gaagatatat ttacattaca acgccgtatc tcattctgga
     2641 caatgaaatg gtaaccagtc ttaggctggc tgcccagagc ggtattgatg taaggataat
     2701 aactccaggt attcctgaca agaagtatgt ttttgcggta acgcgaacct tctacggtgt
     2761 gcttcttgaa accggtgtga aaatttatga gtatgtgcca ggatttatac attcgaaaaa
     2821 tgtggtgtgt gatgacgaga cggcaatagt tggcacaatt aacatggatt tcaggtcgtt
     2881 ttatctgcat tttgaaaacg gtgtatggct gtgtggtacc gattccgtgt ttgatataaa
     2941 gaaagatttc ctggaaactc tggacatgtg cagggaaata acgtatgaag agtggaaaaa
     3001 aagacctttg tactatagat tcatccagtc cattttgcat gtctttgcac ccatgctcta
     3061 gtcttgccta agaaagtctc cggatggtat aattaagcaa gtagattttg tcatacgtct
     3121 gtttttgggg tataatatat tgttataatt ttttgggagt gaatggattg gattttgcat
     3181 accttatagt tggcatatca aaaaacgagg agaaagatct aaaagaactc tacgacaaga
     3241 ctcatcacac tgtctatggt cttgcccttt ctcttgtacg agacagagtg ctggcaaggg
     3301 acattactat cgaaacctac agacgagtaa aacagtttgc caacacatat gatactgata
     3361 ttaacgctga atattggctt ttggatatta ccaaaaacct ttctattaac gcacttcacg
     3421 acaaggacat acatgcggcc ttgaaggaag gaaagctcga caatgtgtca aaagctgtaa
     3481 gagacaccgt gtcatctttg aaaaacgata gaggctccat tattgtcctt aaagccatgt
     3541 ctggtttatc catgaaggag attgcaaatc ttctgtggta ttatcatgca tctgccagaa
     3601 atgagtacaa gcgtggatta aaagaacttc gcaaaaagga taatgtaaaa agaagtgcaa
     3661 gtgatatcct tgaatatatc aggagggata ttacatcctc cgcacctgat gtttatgacc
     3721 agattctttc agacaaacag acaagggtct cttttgtaag tcacgaagta ttaaatctgt
     3781 cggaggatga aattaccttt accgacaagg atgcggagct ggaatatatc agaaggcttg
     3841 ccaaatcagg caagagcgca aaaagagccc gcttgggagg tattgcagcc atggttcttg
     3901 tctgtgtg
//
LOCUS       DAONVY010000184         3902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16324_length_3902_cov_3.177801, whole genome shotgun sequence.
ACCESSION   DAONVY010000184 DAONVY010000000
VERSION     DAONVY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16324_length_3902_cov_3.177801"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="PLI11_05870"
     CDS             <1..1132
                     /locus_tag="PLI11_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPZ52425.1"
                     /translation="LGMCTFANGDRIGAVRIFNSVKDPVMREKALNYLDYLANEMDTI
                     DNRDVVRPEIAEDEMFEFEAGTPTDKQTFKKFEVIEVEDTMENLKDLKIELVVKDVQE
                     EVKVEKVEEVEEVAEEEEVEEEEEIEEKIVNFGGVEKKTNVKQEIYTEEKRKEKPPAK
                     RIKNALTYYFVAGYVICAALMFALFMYAMDTKNENMELKDTLAKYTGSNTTASTPTPE
                     PTQTTSKATPTPTEVPIDHYAETKKLVRECIKNYNFKQYFNVVEIYHTIKMEYVSESD
                     LEYLKKIYEASLNQFTKQNHDSIYALSDQGRYDEVIEMLLPIQKYNPNYSRIDSVIFY
                     IGKAYEDSKNYAKAKEYYTEIVDKYPDSSYYSWASYRLKVIG"
     gene            1148..2647
                     /gene="thrC"
                     /locus_tag="PLI11_05875"
     CDS             1148..2647
                     /gene="thrC"
                     /locus_tag="PLI11_05875"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPZ52426.1"
                     /translation="MQYKSTRGSSKIVPFHQAVLQGIAPDGGLFVPTQEVYFDSLDLA
                     DMSSMTYEALAFTIIKRYVNDDIPDSKLMGFIKNAYYQGAFPPTVAPISRMSKTLSVL
                     ELWHGPTCAFKDMALQILPEFMVGSLAVADEKNEILILTATSGDTGKAALEGFKNKKG
                     IKIMVFYPENGVSEMQKYQMITQEGDNVSVVAVKGNFDDAQTGVKEIFTDKEFNEMLL
                     QKGYRLSSANSINWGRLLPQIIYYFYAYGSLVRQNVIKLGSKVNFVVPTGNFGNILAC
                     YYAMKAGLPVSKLICASNDNNILTDFFNTGTYDKNRKFVTTISPAMDILVSSNLERLL
                     YDVSGEDDAIVSRCMTSLKENGLYSVDKRTINSMNNFMWASYSTEEETKETIKNVYEK
                     YNYLIDPHTAVGINVYDKYVISTGDMTKTICVSTASPFKFGKTVAESLYGSDVVEGMS
                     EFEINRMLSERTNMPIPTPLNDLDKKEVVHEGVCDTAEMKDEVLKFLGI"
     gene            complement(2694..>3902)
                     /locus_tag="PLI11_05880"
     CDS             complement(2694..>3902)
                     /locus_tag="PLI11_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPZ52427.1"
                     /translation="ALELLSPKLYNHHQQTTYMSYIIAEHLGLPANKKRDLFYAALIH
                     DIGTLFGSENLEIAETETVSIKYHGFRGWRLLKNFEPLKHSAQIIKYHHVSYEYGKGM
                     QFENENVPEESHIIHLADRVCMAFAQKKNILSQVGSVLDYIESRAGSVFDPVYVEVLK
                     KAAQKEYIWLNLFSRSPIEHIQEQICDATVLSSDETLEFTQIISHVIDFKSRFTACHS
                     AGVAQIAKEIARLMHFSESECIMMQIAGHLHDIGKLAVSNKILEKDNVLEIHEYAEMK
                     AHTYYTYHLLNIVPEFSIIKEWAAYHHEKLNGNGYPFHLSGENLTLGSRIMTVSDIFA
                     AITEDRPYRKGMEKEKAVNVLEQMVERNEIDKNVVKVLFENYDNILQVRKKAQKAALI
                     EFDKFTAPFV"
BASE COUNT         1214 a          714 c          882 g         1092 t
ORIGIN      
        1 ccttggcatg tgtacttttg caaatggaga cagaattggt gcggtaagga tatttaactc
       61 ggtcaaggat cctgtgatgc gtgaaaaagc acttaattat ctggattatc ttgccaatga
      121 aatggacacc attgacaaca gagacgtggt gaggccggag attgccgagg atgaaatgtt
      181 tgaatttgaa gcgggtacac caacagacaa gcagacattt aagaagttcg aggtaattga
      241 agtcgaggat accatggaga acctgaagga tctaaagata gagctggttg tcaaggatgt
      301 ccaagaagaa gtaaaggtgg agaaagtcga agaggttgaa gaagtagcag aagaggaaga
      361 ggtggaagag gaggaggaaa tcgaggagaa gattgtaaat tttggcgggg ttgagaaaaa
      421 aaccaatgta aaacaggaaa tatatacaga agaaaaaaga aaagaaaaac ctcctgcaaa
      481 gagaattaaa aatgctttga cgtattattt cgttgccggt tacgtgatct gtgctgcatt
      541 gatgtttgct cttttcatgt atgcaatgga tacaaaaaat gagaatatgg agctaaaaga
      601 cactttggcc aagtataccg ggtcgaacac cactgccagc acaccaactc ccgaacctac
      661 gcaaacgaca agcaaagcga cgcctacgcc tacggaagta ccgatagatc actatgcaga
      721 gacaaagaag cttgtaaggg agtgtataaa gaactacaac ttcaagcagt atttcaatgt
      781 agtggagatt tatcatacaa tcaagatgga atatgtgtcc gagtcggatt tggagtattt
      841 gaaaaagatt tatgaggcat ctctgaatca gtttaccaag cagaatcatg attcaattta
      901 tgcgttgtct gatcagggca ggtatgatga agttatcgaa atgctcctcc caatacagaa
      961 atacaatcca aattacagtc gaatagactc cgtaatattc tatattggaa aggcgtatga
     1021 ggactccaaa aactatgcca aagcaaagga atactatacg gagattgttg acaagtatcc
     1081 cgattcatca tattatagtt gggcttcgta tagattaaaa gtgattggtt aggatggtgt
     1141 tgtatagatg cagtataaaa gtaccagagg aagttcaaaa atagttccgt tccaccaggc
     1201 ggttctacag ggaatcgctc cggacggagg actttttgta ccaacacagg aagtatactt
     1261 tgattctctg gatttggctg acatgagttc aatgacttac gaggcattgg cttttacaat
     1321 aattaaaaga tatgttaatg acgatatacc cgacagtaag ttaatgggtt ttataaaaaa
     1381 cgcatactat cagggtgcgt ttccgccaac ggtggcaccc atcagcagaa tgagcaagac
     1441 attgtcagtg cttgaacttt ggcacgggcc aacatgtgct ttcaaggaca tggctcttca
     1501 gattctgccc gagttcatgg ttggcagtct ggcagttgct gatgagaaga atgaaatact
     1561 gatccttact gcaacaagcg gagataccgg taaagcagcc cttgaaggat tcaaaaacaa
     1621 aaagggtatt aaaatcatgg tgttttatcc tgaaaacggt gtcagcgaaa tgcagaaata
     1681 tcagatgatt acacaagagg gagacaatgt ttcagtagtc gccgtaaaag gcaattttga
     1741 cgatgcccag acaggagtta aggagatttt caccgataag gaattcaatg aaatgctttt
     1801 acagaaaggc tacaggctgt cttctgcaaa ctccatcaac tggggaagac tactgcctca
     1861 aatcatctat tatttctacg cctatggaag ccttgttagg cagaatgtaa tcaagctggg
     1921 cagcaaggtg aatttcgtgg tacccacagg caattttggc aacatacttg cctgctacta
     1981 tgctatgaag gcagggctgc ctgtgtcaaa gcttatctgt gcatccaatg acaacaacat
     2041 actgactgat tttttcaata ccggaactta tgacaagaac cgcaagtttg tcacaaccat
     2101 ctcgccggca atggatatac tggtgtcttc aaatcttgaa aggcttttgt atgatgtatc
     2161 cggagaagat gatgcaatcg tatcaagatg catgacatct ctaaaggaaa acggtctgta
     2221 ctccgttgac aagaggacaa tcaacagcat gaacaacttc atgtgggcat cttactcaac
     2281 ggaggaagaa acaaaagaaa caataaagaa tgtttatgaa aagtataatt atctaattga
     2341 tcctcatact gcggtaggta tcaatgtgta tgacaaatat gtcatttcca caggagacat
     2401 gacaaagacc atatgtgtgt ccacagcaag tcctttcaag tttggaaaga cggttgcaga
     2461 gagtctttat ggttccgatg ttgttgaagg aatgagcgag tttgaaatta acaggatgct
     2521 gagtgaaagg acaaacatgc caataccaac gcctttgaat gaccttgaca agaaggaagt
     2581 tgttcacgaa ggtgtatgcg ataccgctga aatgaaggac gaagtattga aattccttgg
     2641 aatataatta tacataaaga aggatcgtga cttttttgtc atgatccttt tttctataca
     2701 aacggtgctg tgaatttatc aaattcgata agtgctgctt tctgcgcttt tttccgaacc
     2761 tgtaaaatgt tatcgtaatt ctcaaaaaga acttttacca cattcttatc tatttcattt
     2821 ctctcaacca tttgttcaag cacattgacc gccttttcct tttccatacc ttttctgtag
     2881 ggcctgtcct cggttatggc agcgaatata tcgcttacag tcattattct ggaacccaag
     2941 gtcaagttct ctccggatag gtggaaagga tagccgttcc cattcagttt ttcatgatga
     3001 taagctgccc attcttttat aattgaaaac tctggaacga tgttcagtag atgatatgtg
     3061 taataggtgt gggctttcat ttcagcgtac tcatgtattt caagtacatt gtccttttca
     3121 agaattttat tgcttactgc aagtttgcca atatcgtgca gatgtcccgc tatctgcatc
     3181 atgatgcatt cagactctga aaaatgcatt agtctggcaa tttcttttgc aatctgtgca
     3241 acaccggcgg agtgacaggc ggtgaacctg cttttaaagt cgataacgtg ggaaataatt
     3301 tgtgtaaact caagtgtctc atctgaggac agaacggtag catcacaaat ctgttcctga
     3361 atgtgctcga taggcgagcg ggagaacaag ttgagccaga tatattcttt ttgtgcagcc
     3421 tttttcaaaa cttccacata aacaggatca aacacactgc ctgctctgga ttcgatgtaa
     3481 tccaataccg agccaacctg gctaagtatg ttcttctttt gtgcaaatgc catgcagact
     3541 ctgtcagcca ggtggatgat atggctttct tctggaacgt tctcgttttc aaattgcatg
     3601 ccttttccgt actcgtagct gacatgatgg tattttataa tctgagcgga atgctttaag
     3661 ggctcaaaat tttttaacag cctccagcct ctgaaaccgt gatactttat ggatacggtt
     3721 tcagtttctg caatttcgag attctcagat ccaaaaaggg ttccaatgtc atgtatgagt
     3781 gcagcataaa aaaggtctct ttttttgtta gcagggagac ccaggtgttc agctatgatg
     3841 tatgacatgt atgtggtttg ttgatgatga ttgtataatt ttggcgacaa tagttccaat
     3901 gc
//
LOCUS       DAONVY010000185         3860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16521_length_3860_cov_2.597898, whole genome shotgun sequence.
ACCESSION   DAONVY010000185 DAONVY010000000
VERSION     DAONVY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16521_length_3860_cov_2.597898"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(642..1490)
                     /gene="folD"
                     /locus_tag="PLI11_05885"
     CDS             complement(642..1490)
                     /gene="folD"
                     /locus_tag="PLI11_05885"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPZ52428.1"
                     /translation="MIVVLIDGKKVSMRIREEIKKEVVELKEKGINPCLSVVLVGSDM
                     ASRTYVNNKKKACAEVGMLSKEFLLPDEASEEELLELIDRLNKDKEVHGILVQLPLPK
                     HIDEMKVIEAIDPKKDVDCFHPYNVGRLYTGSGIFAPCTPAGVMELIKETGTEIEGKE
                     CVVVGRSNIVGKPMAMLLLAANGTVTVTHSRTKDLKSVCKRADILIFAIGKAKFVNEE
                     YVKPGAVVIDVGMNRDENNKLCGDVDFERVKDICGAITPVPGGVGPMTITTLLSNTLL
                     AARMFG"
     gene            complement(1487..3784)
                     /locus_tag="PLI11_05890"
     CDS             complement(1487..3784)
                     /locus_tag="PLI11_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPZ52429.1"
                     /translation="MANSSSQKKKSQNGKTARVKSNKTEHREIVSIVLFATGLLMFLS
                     YIDKVGIVGVLIRGFLFGFFGQVASFLILGLIWYLAWCLLRGTERQVFTFKKTFLFTL
                     LIIMISSLIHTISFDASHEDFFETVRQLIDSHKQKPSGGLLGGGLSLVLQKYMEKAGA
                     LVVLIPLTIIITMVLFSLSIMNFINKVSFIGSKVGRTGRKIYERLRNNKQQSVVIDRQ
                     KIKNAIEESEMLKDEYLLEEQELTFDDEIIEKHEPFINEPEQYTFSPEKIDVEETTFV
                     ENIISKEKHCYLKPPISLLADSDQEQDFSDEQKKHASRVARKIEDILFSFNIDAKVVN
                     ISRGPTITRYDVQPSSGVKVSKIKNLSEDIALNLASPGIRIEQIPGKAAIGVEVPNDA
                     VRVVSLREIIDSKAFRNHRSNTAFCLGCDITGEPVVADIARMPHLLIAGATGSGKSVC
                     INSLIMSLIYKASPDDVKLILIDPKVVELGIYNGIPHLMIPVVTQTKKAAGALSWAVQ
                     EMEKRYTMFAEKGVRDIAGYNRQAKAEGEDKLPKIVIIIDELADLMLAARDSVEDAIC
                     RIAQKARACGIHLVVATQRPSVDVITGLIKANIPSRIAFKVASQVDSRIILDNAGGAG
                     ADKLLGRGDMLFLPVGSIKPIRVQGAFVSDAEVEAVVDYLKKSCEAEYDMEVVKEVEN
                     TQSAEGTRNTALEEDELLPQAVQIAIEMKQISASYLQRKLGIGYNRAARIIDQMEERG
                     YISCKDGSNPRMVLIDKFPEELQGR"
BASE COUNT         1112 a          937 c          609 g         1202 t
ORIGIN      
        1 caacaaatta agccaactag tgaactgaaa actaaaggga cgactatgaa tagaataata
       61 tcgctagttc ctataccgaa cacctccctt atttagttgt caaggtactc atatttatct
      121 gaacaactta aattcttcta aatgcacaaa atgaattgta tgcactttat gcattttgtg
      181 catattatgc attgtgcata atatgcatca cagttaattc taaataaaaa gataatattt
      241 gtcaagaaga aataatgcaa gttacgatca atacaaccta aattatgaaa gcctgcataa
      301 atattgaata ttttggctcg attttgtgaa tataccaacc taatacatta acaatatata
      361 tgataaaata ttttcaaatt aatatgcgaa cataacggaa ggcgtatttc atacaatcct
      421 caatctgagc agtttagact ccccgatgac ggaataatga aggaagttac aatagaacag
      481 tatgaaaatg gcaaagccag agtaacattc aatattactc atacccccta cagtgataat
      541 cagtcaagaa aacaccgtta ctattgagtt taaacatata tttgttcaga agaaatttta
      601 cacaaaacct ggccatcagg ccaggttttt actaacataa atcaaccaaa cattcttgca
      661 gccaaaagtg tattcgaaag aagtgttgta attgtcatgg gacccactcc acctggaaca
      721 ggagtaatgg ctccacaaat atcctttacc ctctcaaagt ccacgtcacc gcacaacttg
      781 ttattctcat ccctgttcat gcccacatca attacgacag caccgggctt tacatactct
      841 tcgttaacga acttggcctt tcctatggca aaaatcagaa tgtcagccct cttgcaaacg
      901 ctttttaaat cctttgttct tgaatgggta accgtgacag taccatttgc agcaagaaga
      961 agcattgcca taggctttcc tactatattg ctcctgccaa caacaacaca ctctttacct
     1021 tcaatttcag ttcctgtttc tttaataagt tccataacac ctgctggagt acaaggagca
     1081 aagattccac ttcccgtata cagccttccg acattatatg gatggaagca gtctacatcc
     1141 tttttgggat caattgcttc aataactttc atctcgtcta tatgtttggg aaggggcagc
     1201 tgaaccagaa ttccgtgcac ttccttgtct ttattcagcc tgtcaatgag ttcaagaagt
     1261 tcttcctcgc ttgcctcatc aggcagaagg aattcctttg acagcatccc gacttccgca
     1321 catgccttct tcttgttgtt tacatatgtc ctcgaggcca tgtccgaacc aaccagaacc
     1381 accgaaagac aaggattaat tcccttttct ttaagctcaa ccacttcctt ctttatctct
     1441 tcccttatac gcattgatac ctttttacca tctattaaca caacaatcac cttccttgca
     1501 attcttcggg aaatttatct atcaaaacca ttcttggatt acttccatcc ttgcaggata
     1561 tatatcccct ttcctccatc tggtcaatta tcctggccgc tctattgtat cctatcccaa
     1621 gctttctttg gagataagag gctgaaatct gtttcatttc aattgcaatc tgtacagcct
     1681 gaggaagcag ttcgtcctct tccaatgcag tatttcgtgt tccttcagca ctttgagtgt
     1741 tctcaacttc ttttacgact tccatgtcat actccgcttc gcatgacttc ttaagatagt
     1801 ctacaaccgc ctcaacctct gcgtcggaaa cgaaagctcc ctgcactctt attggcttta
     1861 tacttcctac cggcagaaac agcatgtcac ctctacccaa aagcttgtcc gctcctgctc
     1921 caccggcatt gtcaagaatg attcttgaat ccacttgcga tgcaactttg aaagctatcc
     1981 ttgacggtat attggcctta atcagtcctg taattacatc aaccgagggc ctttgggtag
     2041 caaccaccag atgtatgccg caggctcttg ccttctgtgc tattctgcat attgcatcct
     2101 caacagagtc ccttgctgca agcatgaggt ctgccagctc gtctataatt atgacaatct
     2161 tgggcagctt atcctcgcct tcagcctttg cctgcctgtt gtaacctgca atgtccctta
     2221 cacctttttc agcaaacatg gtgtatcttt tttccatttc ctgaactgcc caggacaatg
     2281 ccccagccgc cttcttggtt tgtgttacca caggtatcat caagtgaggt atgccattgt
     2341 atataccaag ttcaacaacc ttggggtcta tcagtattaa ttttacatca tcgggagacg
     2401 ccttgtatat taggctcata atcaagctgt taatacagac gctcttaccc gaacccgtcg
     2461 caccagctat caaaagatgc ggcatccgtg caatatctgc tacaacaggt tctcctgtta
     2521 tgtcacaacc aagacagaat gcagtgtttg acctgtgatt tctgaaagcc tttgaatcta
     2581 taatctctct caaagaaaca actcttacag catcattagg aacctccaca ccgattgcgg
     2641 cctttcctgg gatctgctct atccttatac cgggcgatgc cagattaagc gcaatatctt
     2701 ccgatagatt ctttatcttg cttaccttta caccggaact gggctgcacg tcatacctgg
     2761 ttattgtagg tcccctacta atattcacaa cctttgcatc aatgttgaaa ctgaacaaaa
     2821 tatcctcaat ctttctggca acccttgacg catgtttttt ctgctcatcg gaaaaatcct
     2881 gttcctgatc gctgtcagca agaaggctta tgggaggttt taaatagcag tgtttttcct
     2941 tgcttataat attctccaca aatgttgttt cttccacatc aatcttctct ggagaaaagg
     3001 tgtattgctc aggctcatta atgaaaggtt cgtgtttctc tataatttca tcgtcaaagg
     3061 tcagttcctg ttcctcaagc aaatattcat ccttcaacat ctcgctttct tcaatcgcat
     3121 tcttgatttt ctgtctgtca attaccacac tctgctgctt attatttctc aacctctcat
     3181 atatttttct tccagtcctt cccactttgc ttccgataaa tgaaaccttg tttataaaat
     3241 tcattatgga aagcgaaaaa agaaccattg ttataattat tgtcaatggt atcaatacta
     3301 ccaaggcacc agccttctcc atatatttct gcaaaacaag actcaaccct cctccaagca
     3361 agcctccgga aggtttttgc ttatgcgaat caataagctg tcttaccgtt tcaaagaaat
     3421 cttcatggga ggcatcaaac gaaatcgtat gtataagtga cgatatcatt atgattaaga
     3481 gagtaaacag aaaagtcttc ttaaaagtaa aaacctgtcg ttcagtaccc cttaatagac
     3541 accatgccaa ataccatata aggccaagaa ttagaaaaga agcaacttgt ccaaaaaaac
     3601 cgaacaaaaa tcctctaatg agaacgccaa caattcctac cttgtctata tacgaaagga
     3661 acatcaataa gcctgtggca aatagaacaa ttgacacaat ctcacgatgt tccgtcttgt
     3721 tgcttttgac tcttgccgtt ttgccatttt gtgacttttt cttttgtgat gatgagttgg
     3781 ccaaaaatct cactcccaat tattattcaa taatcatttt atcatttatg ccaacaattt
     3841 gcaatattca gctattccgc
//
LOCUS       DAONVY010000186         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16572_length_3849_cov_3.411439, whole genome shotgun sequence.
ACCESSION   DAONVY010000186 DAONVY010000000
VERSION     DAONVY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16572_length_3849_cov_3.411439"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /gene="argS"
                     /locus_tag="PLI11_05895"
     CDS             complement(<1..1649)
                     /gene="argS"
                     /locus_tag="PLI11_05895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPZ52430.1"
                     /translation="MDYKGYIAECISKALNDDLDKVLGLLSVPPNENMGDVAFPCFSY
                     AKQFRKAPAMIAQEVLERIKSDSDFDEKIIAKMEVAGGYLNFFLNRNEFISSVVSDVV
                     EKGVDFARSDEGNNKKVIVEFSSPNIAKPFHIGHLFSTSLGNSIEKIYSYLGYDTVKI
                     NHLGDWGTQFGKLISAYKRWGVDEDILKDPINELLKIYVKFHQKAEENPSLEDEARMY
                     FKKLEDGDEEIVRLWKFFVEVSMKEFSRVYNMLNISFDSYAGESFYQDKMPEVIEMLE
                     EKGLLEDSEGARVVTFGDMPPCIIIKKDGTTIYATRDIAAAIYRKRHYDFHKNIYVVG
                     LPQTLHFKQVFGVVDKMGFGWADDCVHVGFGTVRFPDKKLSTRHGDVVFLEDVLNESI
                     AKTREIIENSSTTKEIENVDEVATKVGIGAILFTFLRNARERDIVFTWEDILDFEGES
                     GPYVQYSYARGKSILRKAEEQNIEYRGAKLDVLDSQEEFALVKLIKDFPEAIRNAANK
                     YEPSIVTRHIVDVAQAFNKFYNSYSILKAEEDVMKARLNIV"
     gene            complement(1658..1918)
                     /locus_tag="PLI11_05900"
     CDS             complement(1658..1918)
                     /locus_tag="PLI11_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52431.1"
                     /translation="MGKMTEFIVTRGDMQTVLWLMIGIILSIIYALARDRAVFFLMVS
                     SYITCIASFSKMTLTSQVITFIVLYLCTYLGLKYFKPNSKKE"
     gene            complement(1935..>3849)
                     /gene="leuS"
                     /locus_tag="PLI11_05905"
     CDS             complement(1935..>3849)
                     /gene="leuS"
                     /locus_tag="PLI11_05905"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPZ52432.1"
                     /translation="NEEVVGGKCERCDGEVVRRVKSQWMLKITEYAEKLLEGLEEVDY
                     IEKVKTQQRNWIGKSKGAEVDFAIPCINEKLTVFTTRPDTLFGATYMVISPEHPILDR
                     MKEHITNFDEVEAYRAEAIKKSDFERSELNKEKTGVSLKGVVAINPVNNTEIPVWISD
                     YVLMSYGTGAIMAVPAHDERDYEFAKKFDLPIIEVVAGGDIENEAFTDIETGTMVNSD
                     FLNGMSVAEAKEAITKWLAEKGLGKEKINYKLRDWVFSRQRYWGEPIPIIHCEKCGYV
                     PVPYDQLPVTLPDIENYQQSDSGESPLVNIPEWVNTTCPKCSGPAKRETDTMPQWAGS
                     SWYFIRYADPHNDDAMIDKKNAAYWLPVDWYNGGMEHTTLHLLYSRFWHRFLYDIGEI
                     PCKEPYKKRTSHGMILGADGQKMSKSRGNVINPDDVVDEFGADTFRLYEMFMGAFDQT
                     TPWSEQGVRGCYRFIDRVWKLQELVTDEEEVSEDHRYLVHTTIKKVSEDYESMKYNTA
                     IAQMMTFVNEVNEKGKLSRGDLRVFVKLLNPVAPHIAEEIWEKMNLGEGYLYEQSWPE
                     YDAKYTVKDEIEIAVQINGKVRERLDVPSKYDKKQIEEFVLANEKVQDLIGDKQIRKI
                     IVVPGRLVNIVVG"
BASE COUNT         1020 a          959 c          645 g         1225 t
ORIGIN      
        1 tctacaatgt ttaatcttgc cttcatgaca tcctcttcag ccttcaatat actgtagctg
       61 ttgtaaaact tgttgaaggc ctgagcaacg tcaacaatat gccttgttac gatagaaggc
      121 tcatatttgt ttgcggcatt cctaatcgct tctggaaaat ctttgataag ttttacaagg
      181 gcaaactcct cctgactgtc gagaacatca agttttgcac ccctgtactc aatattctgt
      241 tcttctgcct ttctcaagat gctctttcca cgagcatagc tgtactgcac atagggaccg
      301 gattcaccct caaaatcaag aatatcctcc caggtgaaca caatatccct ttcccttgca
      361 tttcttagga aggtaaagag aattgcacca ataccaacct tggttgcaac ttcgtcaaca
      421 ttttctattt ccttggtagt cgaactgttt tcaatgattt cccttgtttt tgcaatggat
      481 tcatttaaaa catcttcaag gaacaccaca tctccatgcc ttgtggaaag tttcttgtca
      541 gggaatctta cagtaccaaa tccaacgtgg acgcagtcat cagcccagcc aaatcccatt
      601 ttgtcaacta caccgaaaac ctgcttgaag tgtagagtct gaggcagtcc taccacataa
      661 atattcttat gaaaatcata atgtctcttc ctgtaaattg cagctgcaat atccctggtt
      721 gcataaatgg tggttccatc cttcttgatg attatacaag ggggcatgtc gccaaaggtt
      781 acaactcttg caccttcgct gtcttcgagg agtcccttct cttccagcat ctcaataacc
      841 tctggcattt tatcctggta gaagctttct cctgcatagg agtcaaagga aatgttgagc
      901 atgttgtata ctctggagaa ttccttcatg gaaacttcca caaagaactt ccagagccta
      961 actatctcct catccccatc ttcaagcttc ttaaagtaca ttctagcctc atcctcaagg
     1021 gaaggatttt cctccgcttt ctggtggaac ttaacatata ttttcaaaag ctcattaata
     1081 gggtctttta atatatcctc atcaacaccc caccttttgt aggcagagat aagttttcca
     1141 aattgggtac cccagtcacc caagtgattt atcttgactg tgtcgtatcc caggtaggaa
     1201 tatattttct caatagagtt gcccaaggag gtgctgaaca ggtgtcctat atgaaacggc
     1261 tttgcaatgt tcggagacga aaactcaaca atgacttttt tattgtttcc ctcgtcgctg
     1321 cgtgcaaaat caacgccttt ttcaacaaca tcacttacta ccgaagagat gaattcattc
     1381 ctgttcagaa agaaattcaa gtatcctcct gcaacttcca tctttgcaat aatcttttca
     1441 tcaaaatcac tgtcagactt tattctctcc agtacttcct gtgcaatcat ggcaggagcc
     1501 tttctgaatt gctttgcata tgaaaaacaa gggaatgcaa catcccccat gttttcatta
     1561 ggcggtaccg acaaaagtcc aagcaccttg tcaagatcat cattcaatgc cttgctaata
     1621 cattctgcta tatatccttt gtaatccata agactattta ctcctttttg ctgtttggtt
     1681 taaagtattt caaaccgaga tacgtacaaa ggtacagtac aataaacgta atcacctgtg
     1741 atgtcaatgt cattttcgaa aaagacgcaa tacaggtaat atacgagctc accatcaaaa
     1801 agaacactgc cctgtctctt gccaaggcat agataattga cagtataatc cctatcataa
     1861 gccacagaac tgtttgcatg tctccccttg taacaataaa ctcggtcatt ttacccattt
     1921 tttacctcct caaatcaacc aacaactatg tttacaagtc ttccaggaac aacgataatt
     1981 ttcctaatct gtttgtcccc tatgaggtct tgtaccttct catttgccaa aacaaactct
     2041 tcaatttgtt tcttgtcata ttttgaaggc acatccaatc tttcccttac cttgccgttt
     2101 atctgaacag caatctctat ctcatccttg accgtatatt ttgcatcata ctcaggccaa
     2161 ctttgctcgt atagataacc ttcgcccaag ttcattttct cccaaatctc ttctgcaata
     2221 tggggggcaa cgggattcag aagctttaca aataccctga gatctcctct tgagagtttg
     2281 cccttctcat taacttcatt cacaaaagtc atcatttgag caattgccgt attatacttc
     2341 atgctctcat agtcttcgga aaccttcttt atggttgtgt gtaccaaata tctatggtct
     2401 tctgacactt cttcttcatc agtgaccagt tcctgaagct tccataccct gtcaatgaat
     2461 ctgtaacagc ccctaacacc ctgttctgac cagggagtgg tctggtcaaa agcgcccatg
     2521 aacatttcgt acaatctgaa agtatccgcg ccaaactcat ctacaacatc atcaggattt
     2581 atgacattgc ctcttgactt ggacatcttc tggccatcgg caccaaggat cattccatgg
     2641 gaagtcctct tcttgtacgg ctccttgcat ggaatctctc caatatcata gaggaatctg
     2701 tgccagaatc ttgaatacag aaggtgcagt gtagtatgct ccataccacc attgtaccag
     2761 tcaactggca gccagtatgc agcattcttc ttgtcaatca tcgcatcgtc attgtgtggg
     2821 tcggcgtatc tgatgaagta ccaggaagaa cctgcccact gaggcattgt atcggtttcc
     2881 cttttcgcag gtccactgca ttttggacaa gttgtgttaa cccactcagg tatattgaca
     2941 agaggcgact ctccgctgtc actctgctga tagttttcaa tatcaggaag tgttactggc
     3001 agctgatcat atggaacggg cacataaccg catttctcac agtgtataat gggtatgggt
     3061 tctccccagt agcgctgtct tgagaatacc cagtccctta acttgtaatt tattttttct
     3121 ttgcccaaac ccttttctgc aagccacttt gttattgctt cttttgcttc cgcaacactc
     3181 attccgttaa ggaaatcgga atttaccatg gtgcctgttt caatatcagt aaaagcctca
     3241 ttctctatat cgccaccggc aacaacttca ataataggca aatcaaactt ctttgcaaat
     3301 tcatagtctc tttcatcatg tgcaggaacc gccatgattg ctcctgttcc gtaagacatg
     3361 agtacatagt ctgagatcca aacaggtatt tcagtgttgt ttaccggatt gattgcaaca
     3421 acgcccttaa ggcttacgcc tgttttttcc ttgttcaatt cgcttctttc gaaatccgac
     3481 ttcttgattg cctctgccct gtatgcttca acctcatcaa agttggttat atgttccttc
     3541 attctgtcca aaattggatg ctccggagaa ataaccatgt aggtagcacc aaacaatgta
     3601 tcaggtctgg ttgtaaatac agtcaatttt tcattaatgc agggaattgc aaaatcgact
     3661 tccgcacctt tgctcttgcc tatccagttt ctttgctgtg ttttaacctt ctctatataa
     3721 tcaacttctt caagaccttc aagaagcttc tctgcatact cagttatctt aagcatccac
     3781 tggctcttaa ctctgcgaac aacttcgcca tcgcagcgct cgcattttcc tcccacaact
     3841 tcttcgttt
//
LOCUS       DAONVY010000187         3843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16597_length_3843_cov_3.697994, whole genome shotgun sequence.
ACCESSION   DAONVY010000187 DAONVY010000000
VERSION     DAONVY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16597_length_3843_cov_3.697994"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2138
                     /locus_tag="PLI11_05910"
     CDS             <1..2138
                     /locus_tag="PLI11_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52433.1"
                     /translation="PIISEVVEEGIKSRTWLEWDLNGKMPSLPEAGEYVVHIERSGEG
                     TMTFNIANSSHSKVRLYESYAERGGSLQMRINYTKGGGNMEDISESKMVFNEDFDRYA
                     ATDGLGRVLPGYKEAGPVREGKYVGIFFHTWHDWNASRGTRNITEILKEHPDIVNDYN
                     SLYWGDSPVYHWNEPIYNYYKNTDRWVLRKQAELLAEAGVDVIIFDNTNGATTYLDVV
                     EVLLEEFAKARQDGVKTPQISFLMNFFESSFGDTVKQLKELYNAIYRDGRYKDLWFYW
                     KGKPLMMAYPDKLNENDPLEKEIKEFFTFRPGQPSYTMGQQFVGQWGWLSIYPQQVFY
                     NEDGTPEQITVGIAQNHSKKRGLTAFNGESVFGRTWSGKLDDYDKRENAKLYGANFAE
                     QFEYALEVDPEFIFITGWNEWVVSRYKEWGGVVNAFPDSFNDEYSRDIEPSRGDLKDH
                     YYYQMVSYIRRFKGVKPPMETGDKKAIDIYSDEDQWADVLPEFKAYPGNTVHRDSSGY
                     INNETRKTYRYSNTTGRNDIRIAKVARDDKFVYFMVECVEDITPPTDEKWMRLFLDIN
                     GKETANWETFNYVVNRVNPVNNKAVLEKSTGGYNWEKVGEVDFSLKGKRLQIKIPKSM
                     LGIKGDKFTINFKWSDNMQVEGDIMDFYVNGDAAPVGRYKFQYTTIDAANNKRSGKVF
                     VYAGGALVLCALALTGFIIARKKKRANKG"
     gene            complement(2189..2815)
                     /gene="safA"
                     /locus_tag="PLI11_05915"
     CDS             complement(2189..2815)
                     /gene="safA"
                     /locus_tag="PLI11_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SafA/ExsA family spore coat assembly protein"
                     /protein_id="HPZ52434.1"
                     /translation="MKRRFKQILIVVALIIILQSTLFANAQGVTYTVKKGDSMWKIAV
                     KYEIGISELIKANPQIKNPNLIYVGQKIYIPEASSLKSYEDEVVRLVNVERAKYGLPA
                     LKSHWELARVARYKSQDMIDKNYFSHQSPTYGSPFNMMESFGLRFSSAGENIAYGQRT
                     PQEVVNAWMNSPGHRANILSKTYTHIGVGAAKKSNGTLYWTQMFMKPY"
     gene            2997..>3843
                     /locus_tag="PLI11_05920"
     CDS             2997..>3843
                     /locus_tag="PLI11_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017688658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52435.1"
                     /translation="MSKKLRTAIIGCGGIANGKHMPAIKSTGVADIVAFCDIIEERAV
                     DAKNRYGTPDSKVYTDYKELLKDESIDVVHVCTPNSSHAPIAIDALNAGKHVLCEKPM
                     AKTAAEAVAMVEAARKNNRKLSIGYQNRFRPDVQYMKRAAEAGELGEAYFAKAHAIRR
                     RAVPTWGVFMDEEKQGGGPLIDIGTHALDMTLWIMDNYKPKYVVGTTYRKLADQTETG
                     NAWGDWDPKEFTVEDSAFGFVVMENGATIILESSWALNTLDIGEAECSISGTLAGIDN
                     RGGKVK"
BASE COUNT         1225 a          635 c          928 g         1055 t
ORIGIN      
        1 cgccaataat atctgaagta gttgaagagg gcattaagtc aagaacatgg ctggagtggg
       61 atttgaacgg aaaaatgcca tcgctgcctg aagctggaga atacgttgta catattgaaa
      121 ggtctggtga aggaaccatg acatttaata ttgcaaatag ttcacacagc aaagtcaggc
      181 tgtatgagtc ttatgcagaa agaggcggaa gcttgcaaat gagaatcaac tacacaaagg
      241 gaggtggcaa tatggaagac atatcagaaa gcaaaatggt tttcaatgag gattttgaca
      301 gatatgcagc aacggacggt cttggtagag tgctgccagg gtataaggaa gctggtcctg
      361 taagggaagg gaaatacgtt ggtatattct ttcacacatg gcacgattgg aatgcatcaa
      421 gaggtacaag aaacataact gaaatactga aagagcatcc tgacatagta aacgattata
      481 acagtcttta ctggggagat tcgcctgtat accactggaa tgagcctata tacaactatt
      541 acaagaatac cgacagatgg gttttgagaa agcaggcaga actgctggca gaagctggag
      601 tggatgtaat cattttcgac aataccaatg gagccaccac atatctggat gttgtggaag
      661 ttttacttga ggagtttgca aaagcaagac aggacggagt aaaaacacct caaatatcgt
      721 ttcttatgaa tttctttgaa agttcatttg gagatacggt caaacagctg aaggaactgt
      781 ataacgcaat ctacagagat gggagataca aggatttatg gttttattgg aaaggcaagc
      841 ctcttatgat ggcgtatcca gacaaactca atgaaaatga tcctttggaa aaagaaataa
      901 aagagttctt tacattcaga ccaggtcagc catcgtatac catgggacag cagtttgtag
      961 gtcagtgggg ctggctcagc atatatccac aacaagtctt ttataatgaa gacggcactc
     1021 ctgagcagat aactgttggt attgcacaga atcacagcaa aaagagggga cttaccgcct
     1081 tcaatggtga gagtgttttt ggaagaacct ggtcaggaaa gctggatgat tacgataaga
     1141 gagaaaatgc aaagctttat ggtgcaaact ttgcggagca atttgagtat gccctggaag
     1201 ttgatcctga gttcattttc attaccggat ggaatgaatg ggttgtaagc aggtacaagg
     1261 aatggggagg cgtagtgaat gcttttccag actccttcaa tgacgaatac tcaagagaca
     1321 tagaaccttc gaggggcgat ttaaaagatc actactatta ccagatggta agttacataa
     1381 ggcgtttcaa gggtgtaaag ccgccgatgg aaactggtga taaaaaggcc atagacattt
     1441 attcagacga agaccagtgg gcggatgtct tgcctgagtt caaagcgtat cctggtaaca
     1501 cagtacacag agacagtagc ggatatatta acaatgagac aagaaaaaca tatagatact
     1561 ccaataccac aggcagaaat gacataagga ttgcgaaagt ggcaagggat gataagtttg
     1621 tttacttcat ggtggagtgc gtggaggata taactccgcc tacggatgaa aaatggatga
     1681 ggttgttcct tgatattaat ggcaaggaaa ctgcgaactg ggagactttc aactatgttg
     1741 taaacagggt gaatcctgtt aacaacaaag ctgttcttga aaagagtact ggaggataca
     1801 actgggaaaa ggttggagag gttgatttta gccttaaggg aaagaggctg cagataaaga
     1861 tacccaagtc catgttgggt ataaagggtg acaaatttac cattaacttt aaatggtccg
     1921 acaacatgca ggttgaagga gacataatgg acttttatgt aaatggtgat gcggctcctg
     1981 ttggaaggta taagttccag tacactacaa ttgatgctgc aaacaataaa cgaagtggaa
     2041 aagtgtttgt atatgcagga ggagctttgg tgctttgtgc tttggctttg acgggtttta
     2101 ttatagcaag gaagaaaaaa agagcaaata aagggtaaag ggcgtcgcat tagtttggaa
     2161 tttgactaat gcgacagcct ttttgttgct aatatggctt cataaacatt tgtgtccagt
     2221 aaagggttcc atttgatttc tttgcagctc ccacacctat gtgagtgtag gtttttgaaa
     2281 gaatgtttgc cctgtgtcct ggtgagttca tccaggcgtt aacaacctcc tgtggagtcc
     2341 tctgaccata tgcaatgttt tcacctgcag aggaaaacct gaggccaaaa gattccatca
     2401 tattaaatgg agaaccatat gttggagatt gatgtgagaa gtagttttta tctatcatgt
     2461 cttgtgattt gtaccgagca actcttgcca gctcccagtg ggatttgagt gcgggcagtc
     2521 cgtattttgc cctttctaca ttcacaagtc gtacaacttc atcctcatag gattttaatg
     2581 aacttgcttc aggtatatat attttctgtc caacatatat caggttggga tttttgattt
     2641 gagggtttgc ttttatcaat tccgaaatac caatttcgta tttgacagca attttccaca
     2701 ttgagtctcc tttttttaca gtgtaggtaa ctccctgcgc atttgcaaaa agtgtactct
     2761 gcaaaataat gataagagct acgacaatta aaatctgttt gaatcttctt ttcaaaatat
     2821 cacctccgca cttatttttg ctgttgctgt tatatatatt ctataaattt aatggaatta
     2881 gaaataaaaa cacatgatgt atttttacag tttgatttgc tttttgattt gctttacaaa
     2941 aggacatgtt ggtgttaaaa ttatcgtaaa tacaacatga gtaaggagtg tatcagatgt
     3001 caaagaaact tagaacagct attattggtt gcggtggaat agcaaacggc aagcatatgc
     3061 cggccattaa atcaacaggt gttgcggata ttgttgcttt ttgcgacatt attgaagaaa
     3121 gagcagttga tgcaaagaac agatatggca caccagacag taaagtatat acagattata
     3181 aagagctttt aaaagatgag agtattgatg ttgtacatgt atgtacacca aacagttcac
     3241 atgcacctat tgctattgat gcattgaatg caggcaagca cgttctgtgt gagaagccaa
     3301 tggcaaaaac agcagctgaa gctgttgcaa tggttgaagc ggcaagaaag aacaacagaa
     3361 aactttcaat tggctaccag aacagattcc gtcctgacgt tcagtacatg aagagggctg
     3421 ctgaagctgg tgagcttggt gaagcatatt ttgcaaaggc tcatgctatt agaagacgtg
     3481 cggttccaac ctggggcgtt ttcatggatg aggaaaaaca gggtggaggt cctctcattg
     3541 atattggtac tcatgctctt gacatgactc tctggattat ggacaactac aaaccaaagt
     3601 atgttgttgg aacaacatat cggaagcttg ctgatcagac tgaaacaggc aatgcatggg
     3661 gcgactggga tccaaaggaa tttacagttg aagactctgc ttttggcttc gtagttatgg
     3721 aaaacggtgc aacaatcatt cttgaatcaa gctgggctct taacactctt gatattggag
     3781 aagcagaatg cagtatttca ggtactttgg caggtataga caacagaggc ggaaaagtaa
     3841 aag
//
LOCUS       DAONVY010000188         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16630_length_3837_cov_3.324167, whole genome shotgun sequence.
ACCESSION   DAONVY010000188 DAONVY010000000
VERSION     DAONVY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16630_length_3837_cov_3.324167"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLI11_05925"
     CDS             <1..578
                     /locus_tag="PLI11_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52436.1"
                     /translation="GLPEEVVSVLSNVAHDNAEVEDISVSILKYPQGTLGQITSSVVH
                     HGEEQKLIFQCENAKVSVPWSLYASKSLGSGFPQRDVDLEKLIQAEYDKIPDLKYEGH
                     VGQIDDVLTAIENNSVPLIKGVDGRKTLELITAIYKSGCTNQCVKLPLDKKDPFYTVE
                     GILKNAKYFNEKKTSKLNLDSQGVNLGSDYR"
     gene            591..1694
                     /locus_tag="PLI11_05930"
     CDS             591..1694
                     /locus_tag="PLI11_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52437.1"
                     /translation="MKANDGMNYAPTGKPHPVCEKGEFVFAAMALEHGHIYGMCNGLL
                     EAGGELKYVYDKDPQKVKSFVERYPFVKVASSEEEILEDESVKLIAAAAITSERCDLG
                     LKVMEHGKDYFTDKAPFTTLEQLERARKKVKETNRKYMVYYSERLHVESAVFAEQLIE
                     EGAIGDVIQVIGLGPHRLNAPLRPKWFFEKEKYGGILCDIGSHQIEQFLFFGKVEDAE
                     VVRSHVANFAHPEYPELEDFGEAMLLGNNGVVNYFRVDWFTPDGLGVWGDGRTIILGT
                     SGYIELRKYTDIARDPKGDNVYLVNHKGEKYFNVHGKVGFPFFGRLILDCLNRTENAM
                     TQEHAFKAAELCLKAQAQAVRIERERKYMYDIK"
     gene            1681..3210
                     /locus_tag="PLI11_05935"
     CDS             1681..3210
                     /locus_tag="PLI11_05935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HPZ52438.1"
                     /translation="MILSNEGIKNKDLWTDKKYKLPVYDRDKMISETKCSPVWIHFGA
                     GNIFRAFLANLQEELLAKGIEKAGIVVVEAYDDEIIDKAYRAYDNLCMLFTLKASGGV
                     DKKVIGSVAESLKAVEDWDRLVEIFENSSLQMASFTITEKGYEDTSENSVLSMIAKLC
                     YRRYKKGKYPISLVSFDNCKDNGKKLFDRVVYHAQRLVEDEGFMEYLANENYVAFPWS
                     MIDKITPRPDEGILKTIAEDGIESIDIYCTSKNTYFSAFVNAEETEYLVIEDKFPNGR
                     PRLEESGVIFSDRETVSLVERMKVSTCLNPLHTALAIFGCLLGFETIYEEMQDEDLAR
                     LVHTLAYKEGLPVVEDPKVINPEDFLKEVIEDRLSNPFIKDSPKRIATDTSQKLGIRF
                     GETLKAYARYEYLDVGNLVAIPLVFAGWCRYLMGVDDEGCEFEISPDPLLETLQEHLK
                     GVKLGSTGPFGKYLKPILCDKSIFGVDLYEIGLGNKVEGYFEKMVSGRHMVRKTLRDS
                     I"
     gene            complement(3236..>3837)
                     /locus_tag="PLI11_05940"
     CDS             complement(3236..>3837)
                     /locus_tag="PLI11_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPZ52439.1"
                     /translation="FLSCFSGPVFGQITALIDWQANNGERLLQETRSMRIYNMPNDSR
                     YIDLVCQLHATEGDVEFSDTKEGGFPFIRVNEEINGHHTGQILSSNNDVGESSVFGKE
                     ADWVDYTGKIKIGQNYVTAGLSVFLHPTSDDYPAKWFVRDYGPFTPSNFHFCGGHTIK
                     KGDKYTFKQRLYIHKNHPEEAFVNRRYEEYKDVLKAAWT"
BASE COUNT         1176 a          676 c          931 g         1054 t
ORIGIN      
        1 tgggtctgcc tgaggaagtg gtctcggtat taagcaatgt ggctcacgac aatgctgaag
       61 tggaagacat ctccgtatct attctcaagt atccgcaagg cactttgggg cagatcacca
      121 gctccgttgt gcaccatggt gaggagcaga aactaatatt ccaatgtgag aatgcaaaag
      181 tatcagttcc ctggtccttg tatgcttcaa aatcactagg cagcggtttt ccgcaaaggg
      241 atgtggatct ggaaaaactc atccaggcag aatacgataa aatacctgat cttaagtatg
      301 aaggacatgt aggacagata gatgatgtct tgaccgctat tgagaataac agcgtgcctt
      361 tgattaaagg tgttgatggt agaaaaacat tggaacttat aactgcgata tacaagtcgg
      421 ggtgtacaaa ccagtgtgtg aaactgcctc ttgacaaaaa agatcctttc tatacagtag
      481 aaggcatatt aaagaatgcc aaatatttta atgaaaagaa aaccagcaaa cttaatctgg
      541 attcccaagg agtcaattta ggcagtgatt acaggtaagg aggaagataa atgaaagcaa
      601 acgatggaat gaattatgct cctacaggca aaccacaccc tgtatgtgaa aaaggagagt
      661 ttgtatttgc tgccatggcc ttggaacacg gccatattta cggcatgtgc aacggacttt
      721 tggaggcagg aggagaactc aaatacgtat atgacaagga ccctcaaaag gtgaaaagtt
      781 ttgtagaaag atatcctttt gttaaagtgg cttcaagtga agaggagatc cttgaagacg
      841 agagtgtaaa actcattgca gctgctgcaa taacatctga aagatgtgat cttgggctta
      901 aagtgatgga acatggaaaa gactatttca ccgacaaggc tccctttacc actttggagc
      961 agctggagag ggcaaggaaa aaggtaaagg aaaccaacag gaagtacatg gtctattaca
     1021 gtgaaagact tcatgtggaa agtgcggtat ttgcagagca gttgattgaa gagggtgcaa
     1081 taggggatgt tatacaggta ataggtcttg ggcctcatag acttaatgca ccgctgcgtc
     1141 caaagtggtt ttttgaaaag gagaaatacg gcggcatatt atgtgatata ggaagccatc
     1201 agattgaaca gttccttttc tttgggaaag tggaggatgc ggaagttgtc agaagtcatg
     1261 tggcaaattt tgcccatcct gaatatccgg agctggagga ctttggagaa gccatgcttc
     1321 ttggcaataa tggtgtcgtc aactatttca gggttgactg gtttacacct gatggattgg
     1381 gagtatgggg tgatggaaga accataattc ttggcacctc cggatatatt gaacttagaa
     1441 aatatacaga catagccagg gatcccaaag gagacaatgt gtatcttgtc aaccacaagg
     1501 gagaaaagta ttttaatgtg catggaaaag taggcttccc gttctttgga cgacttattc
     1561 ttgactgcct taacagaact gaaaatgcca tgacccagga gcatgccttc aaggctgctg
     1621 aattatgcct taaagcccag gcgcaggctg taaggataga aagggaaagg aagtatatgt
     1681 atgatattaa gtaatgaagg cataaaaaat aaagatttat ggactgacaa aaagtacaag
     1741 cttcctgtat atgacaggga caaaatgatt tccgaaacca aatgcagccc tgtgtggatt
     1801 cactttggtg caggcaacat attcagggca tttcttgcaa atttgcagga agagcttctg
     1861 gcaaagggta ttgaaaaagc agggattgtt gttgtggaag cctatgatga tgagataata
     1921 gacaaagcct acagggcata tgacaacttg tgcatgttat tcaccttgaa agcaagcggt
     1981 ggtgttgaca aaaaagttat aggttcggta gctgaatcct tgaaggcggt agaggactgg
     2041 gatcgtcttg tagagatatt tgaaaattcg tctcttcaga tggcaagctt cacaataact
     2101 gaaaaaggct atgaggatac atcggaaaac agcgtgcttt caatgattgc caagctgtgc
     2161 taccgccgtt acaaaaaagg caagtatcca ataagtcttg ttagttttga caactgcaag
     2221 gataatggca agaaactctt tgacagggtt gtttatcatg cacagcgcct ggtggaggat
     2281 gaaggattca tggagtatct ggctaatgag aattacgtgg catttccttg gagcatgatt
     2341 gacaagataa ctccaagacc ggatgaaggc atattaaaga cgattgcaga ggatggcata
     2401 gaatccatag atatttattg cacatcaaaa aatacttatt tttctgcttt tgtcaatgcg
     2461 gaagaaacgg aataccttgt cattgaagac aagtttccca atggacgacc aaggcttgag
     2521 gaaagtggtg tgattttttc tgacagggaa accgtatctc ttgttgaaag aatgaaagtt
     2581 tcaacatgtc ttaaccctct tcatactgct cttgccatct ttggctgtct tcttgggttt
     2641 gaaacaatat atgaggagat gcaggatgaa gatcttgcaa gacttgttca tactcttgca
     2701 tacaaggaag gtttgcctgt ggtagaggat cccaaagtta taaatcctga ggattttctc
     2761 aaggaagtta ttgaagacag attatcaaat ccatttatta aagacagccc caaaaggatt
     2821 gccacagata cttcgcaaaa actaggcata aggtttgggg aaacccttaa ggcatatgca
     2881 aggtatgaat atcttgatgt tggcaacctt gttgcaatac ccctggtatt tgcaggatgg
     2941 tgtcgatatc ttatgggggt tgacgatgaa ggttgcgagt ttgaaattag tcctgatcct
     3001 ttgcttgaaa cactgcaaga gcatttaaag ggcgttaaat tgggtagcac aggacccttt
     3061 ggcaaatatc tgaaacccat actttgcgat aaaagtatat ttggagttga tctatacgag
     3121 ataggtcttg gaaacaaggt ggaaggatac tttgaaaaaa tggtatcggg aaggcatatg
     3181 gtgcgaaaga cccttcgtga cagcatataa tacccggaaa gatatccggg tattttcatg
     3241 tccaagctgc ttttaaaaca tccttgtact cctcgtagcg cctgtttaca aatgcttctt
     3301 caggatggtt cttgtgtatg tatagtcttt gtttgaatgt gtacttgtcg ccttttttta
     3361 tggtatggcc gccacaaaaa tggaagttgg atggagtaaa ggggccgtag tctctaacaa
     3421 accatttggc aggataatcg tccgatgttg gatgtagaaa gacagaaagc cctgcggtta
     3481 cataattttg tcctatctta atctttcctg tatagtcaac ccagtctgct tcttttccaa
     3541 agacactgct ttctcccaca tcattgtttg acgacagtat ttgtcccgtg tgatgaccgt
     3601 ttatctcttc attaacacgg atgaaaggaa aaccgccttc ttttgtgtca ctgaactcaa
     3661 catctccttc ggttgcatgc agctggcaga caagatcaat atatcttgaa tcgtttggca
     3721 tgttatatat tctcatgctt ctggtttcct gtagaagtct ttcaccattg ttggcttgcc
     3781 aatctatgag ggcggtgatt tgaccgaaaa ccggtccgct aaaacaggaa aggaaac
//
LOCUS       DAONVY010000189         3826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16686_length_3826_cov_4.141872, whole genome shotgun sequence.
ACCESSION   DAONVY010000189 DAONVY010000000
VERSION     DAONVY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16686_length_3826_cov_4.141872"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLI11_05945"
     CDS             <1..219
                     /locus_tag="PLI11_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPZ52440.1"
                     /translation="NQLPIVYNIAWYEQKAVIVLLALLYLGVKNIHLGPTLPAFLSPN
                     VAKVLVENFGISGIGTVEEDIENLVKAG"
     gene            411..1220
                     /gene="zupT"
                     /locus_tag="PLI11_05950"
     CDS             411..1220
                     /gene="zupT"
                     /locus_tag="PLI11_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPZ52441.1"
                     /translation="MDLGFSNTIIAFGITLLAGLATGIGSLLAFYTKRTNEKFLAATL
                     GFSAGVMIYVSLVEIFAEAKSSLIEELGAKKGYWVCVISFFAGIALIALIDKLVPQKE
                     NPHEMRDVNEMDKKVDNDSLLRMGLFSALAIAIHNFPEGIATFMSAIQDPKLGISIAV
                     AVAIHNIPEGIAVSIPVYYATGNRKKAFMYSFLSGLSEPLGAIVGFILLRRFISPSLF
                     GIIFASVAGIMVYISLDELMPTAEKYGHHHISIYGLIAGMAVMALSLLMFA"
     gene            complement(1206..1361)
                     /locus_tag="PLI11_05955"
     CDS             complement(1206..1361)
                     /locus_tag="PLI11_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52442.1"
                     /translation="MFEDKIPSRPMKPEIYPPDPLKRPIIPDKPKENEPDTIPNAEPI
                     VNQLGEH"
     gene            complement(1458..1760)
                     /locus_tag="PLI11_05960"
     CDS             complement(1458..1760)
                     /locus_tag="PLI11_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52443.1"
                     /translation="MNRKSKKKKTYNDDDGRVIANMDFEYEPGLKWFAPLKRERPKAE
                     KESEKKNEVDMNPQPPLSRKETFIIMRSALLAGLVIGTVFILIYFLVILFFYLLWS"
     gene            complement(1723..2775)
                     /locus_tag="PLI11_05965"
     CDS             complement(1723..2775)
                     /locus_tag="PLI11_05965"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HPZ52444.1"
                     /translation="MTTNNDVKDKNVEEQPIIEVKDLKKYFPIKSGVIQRTVGHVKAV
                     DGISFSINKGSTMGLVGESGCGKSTAGRTILRLLEKTSGNVYFKGEDVFAVNKKRLRE
                     LRPKMQIIFQDPYSSLSPRLPVGEIIGEAVRQHKIVSDAEYNDYIDDIMNNCGLMPYH
                     KDRYPHEFSGGQRQRICIARALALNPEFIVCDEPVSALDVSIQAQIINLLMDLQEKYN
                     LTYLFISHDLSVVEHISDTVAVMYLGNIVEYGKKEDIFSNPLHPYTEALFSAIPVPDP
                     NYKMKRIVLSGALPSPANPPSGCKFHTRCPKKMEICSKVAPTPIEMEKDHYVVCHLYN
                     KETENEPEVKEEENVQ"
     gene            complement(2762..>3826)
                     /locus_tag="PLI11_05970"
     CDS             complement(2762..>3826)
                     /locus_tag="PLI11_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52445.1"
                     /translation="ELERMRSLMTRDDYITEMKDKNNVLEIDNLHTYFFTDIGVVKAV
                     NGVSFSIPKGKTVGVVGESGCGKSVTSLSVLQLVQRPQGQIVDGAIRFNDEDIAYDIA
                     KMPVDEMQKIRGNKISMIFQEPMTSLNPVFRIGAQINEVIELHNPELDQEKVTARTIE
                     LLDLVGIANSGGVYKMYPHELSGGMRQRVMIAMALACNPKLIIADEPTTALDVTIQAQ
                     ILDLLKNLKDKINSSIMLITHDLGVIAEMADYVVVMYAGKVVEQGLASEVFSNPSHPY
                     TIGLMNSKPSINKKVNRLYSIPGKVPNPINMPNYCFFRDRCDRAFEKCAGPYPDTYSL
                     TDSHSVACYHYEKEERDDNK"
BASE COUNT          990 a          794 c          776 g         1266 t
ORIGIN      
        1 aaccagctcc caatagtgta taacatagca tggtatgagc agaaagcagt cattgtactt
       61 ctggcactat tataccttgg agttaaaaac atccatcttg gtccaacact tcctgcattc
      121 ctatcaccga atgttgctaa agttctggtt gagaactttg gtatatcagg aataggaacc
      181 gtggaggagg atattgagaa tcttgtaaag gcaggctaag atggtttata tagatggagc
      241 agtgggattg ttgtcctgct gcttttttgt ttatagcggt tttaactgaa aatcagcaag
      301 aactctccca tcctcaataa catactaaac aaatctcata gtctattttg aattatctcc
      361 cgttctggta taatatacgt atgcaagtac aatgaacaag gagtgacggt atggacttgg
      421 ggttttctaa cactataata gcctttggaa taactttatt ggcaggtctt gcaacaggaa
      481 ttggaagctt gcttgcattc tacacaaaga ggacaaatga gaagtttctt gccgctacat
      541 tgggtttttc tgcaggtgtt atgatttatg tgtccttggt tgagattttt gcagaggcaa
      601 aaagctccct tattgaagaa cttggagcta aaaagggcta ttgggtttgt gtgatatcct
      661 tttttgcggg catagcatta attgcgttaa tagacaaact tgttccacaa aaagaaaacc
      721 ctcatgaaat gagggatgtg aatgaaatgg ataagaaagt tgataatgat tcacttctta
      781 gaatgggtct tttttcagca cttgccattg caatacacaa ctttcctgag ggtattgcaa
      841 cattcatgag tgcaatacag gatccgaaac taggtatcag tattgccgta gcagttgcaa
      901 tacacaatat tccggaggga atagcagtat ccatccctgt atactatgcc acaggaaata
      961 gaaagaaggc tttcatgtat tcctttttgt ccggtctgtc agagcccttg ggagcaattg
     1021 taggattcat acttcttagg aggtttatct ctccatcgtt attcggaatt atatttgctt
     1081 cagttgcagg cattatggta tatatttctc ttgatgagtt aatgcctacg gccgagaaat
     1141 atggtcacca ccatatttca atttacgggc tgattgcagg aatggctgta atggctttaa
     1201 gtttattaat gttcgcctaa ttgattaact attggctccg catttggtat tgtatctggt
     1261 tcgttttcct ttggtttgtc tggtattatt ggtcgcttta aaggatctgg aggatatatt
     1321 tcaggtttca ttggtctgct tggtattttg tcttcaaaca taatagtcat cctttcatgt
     1381 ttttgttagt ttatcttcta tggatgaaat tatacataaa aaaaccacca tctgctttcg
     1441 caggtggtgg ttttttgtca actccaaaga aggtaaaaaa acagaatgac taaaaaatat
     1501 attaagatga aaacggttcc gattacgagc cctgcaagta gggcggacct cattatgata
     1561 aacgtttctt tcctagaaag aggaggctgt ggattcatat ccacttcatt cttcttttca
     1621 gattcttttt ccgcttttgg tctttctctc tttagtggag caaaccattt aaggcccggt
     1681 tcatattcga aatccatgtt ggctataact ctgccgtcat catcattgta cgttttcttc
     1741 ttctttgact tccggttcat tttccgtctc cttgttgtac agatggcaga caacatagtg
     1801 gtctttttcc atctctattg gcgttggagc aacttttgag caaatctcca ttttctttgg
     1861 acaccttgtg tggaacttgc atcctgaagg aggatttgct ggtgatggaa gcgctcctga
     1921 aagaactatt ctcttcatct tatagtttgg atctggtaca ggaatagcac taaacagagc
     1981 ttccgtatat gggtgaagag gattactgaa gatgtcttct ttcttgccgt actcaacaat
     2041 attacccagg tacatgactg caacggtatc ggatatgtgc tcaaccacgg acaggtcatg
     2101 agatatgaac aggtaagtaa ggttgtactt ctcctgcaaa tccattaaga gatttataat
     2161 ctgtgcttga atcgacacat ccagagcgga taccggctcg tcacacacaa taaactcagg
     2221 attcagagcc aatgctcttg caatgcatat tctctgtctt tgtccacctg aaaactcgtg
     2281 cggatatctg tctttgtggt aaggcataag tccacagttg ttcatgatat catcaatata
     2341 gtcgttgtat tccgcatccg atactatctt gtgctgcctt actgcttccc ctatgatttc
     2401 tcctacagga agtcttggtg agagggaaga gtaggggtcc tggaagatta tctgcatctt
     2461 tggtcttaac tctctcagcc ttttcttgtt gactgcaaag acatcttctc ccttaaagta
     2521 tacattgcct gaagtctttt caagaagcct cagaatggtt cttcctgctg tggacttgcc
     2581 gcatcctgac tcaccgacaa gacccattgt ggaaccttta tttatgctga aactaattcc
     2641 atccacagct ttaacatgtc ctaccgtgcg ctgtattaca cctgacttta ttggaaagta
     2701 tttctttaaa tctttaactt ctattattgg ctgttcttcg acattcttgt cttttacatc
     2761 gttatttgtt gtcatctctt tcctcctttt catagtgata gcatgcaaca ctgtgagaat
     2821 ccgtcaggct gtaggtatct ggatacggcc cagcacactt ttcaaaggcc ctgtcgcatc
     2881 tgtctctaaa gaaacagtag tttggcatgt tgattggatt gggcaccttt ccagggatgc
     2941 tgtataacct gttaactttc ttgttgatgg aaggcttgct gttcataagc cctattgtat
     3001 aagggtggga aggattcgaa aatacttccg atgcaagtcc ttgttcaacg accttgcccg
     3061 catacattac taccacgtaa tcagccatct ctgctataac acccaggtcg tgggtaatca
     3121 gcataatact gctgtttatc ttgtccttaa ggtttttcaa caaatcaagt atctgtgcct
     3181 gaattgtaac gtcaagagcg gttgtaggct cgtcggcaat gataagcttt ggattgcatg
     3241 ccagcgccat ggctatcata actctttgcc tcattccgcc ggacaattcg tgaggataca
     3301 ttttgtatac tcctccgctg tttgcaatac caaccaagtc caaaagctca attgttcttg
     3361 ccgttacttt ttcttgatca agctctgggt tgtgcagttc aattacctca tttatctgag
     3421 ctcctattct aaagacagga ttaaggcttg tcatgggctc ctggaatatc attgatatct
     3481 tattgcccct tattttctgc atttcatcaa cgggcatttt agcaatatca tacgctatat
     3541 cctcgtcatt gaagcggatc gctccatcaa caatttgccc ctgtggtctc tgaacaagct
     3601 gcagaacaga caagctggta actgacttgc cgcaaccgga ctctccaaca acacccacgg
     3661 tttttccctt ggggatgctg aacgaaactc cgtttactgc cttcacgaca ccaatgtctg
     3721 taaagaagta tgtatggaga ttgtctatct ccaatacatt gtttttatcc ttcatttcag
     3781 taatatagtc atccctggtc atcagagagc gcattctttc aagttc
//
LOCUS       DAONVY010000190         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16711_length_3822_cov_3.429785, whole genome shotgun sequence.
ACCESSION   DAONVY010000190 DAONVY010000000
VERSION     DAONVY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16711_length_3822_cov_3.429785"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLI11_05975"
     CDS             complement(<1..494)
                     /locus_tag="PLI11_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPZ52446.1"
                     /translation="MIYIHTALSVEASPIIEGFKLKKDMDTKEFQVFGNEDVILIVSG
                     VGKVKCAMGIAYLFGKYGVCEKDILLNVGYCGSSNLDVPLGTLKLANKVVDMDTGREY
                     FPDLFVTGYEQECISCFSNVITNRKENTEGVFVDMESAGVMEASYKFLYPHQTVIVKI
                     VSDY"
     gene            complement(506..1174)
                     /locus_tag="PLI11_05980"
     CDS             complement(506..1174)
                     /locus_tag="PLI11_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPZ52447.1"
                     /translation="MKTSIKAEALVFDMDGTLLDSLYYWRNTDLYAAKAFNFEPNKNI
                     KLSEQPGSFFERVKNYVNLHGFDHDLDTIKEELHRVAIEFYTTEVTPKHKAVNFLEHV
                     LSQGKRIGIATATNGRVAKIALEHLGLLEYFEFVLSNDDIGVSKRHPDIYLLAAEKLG
                     QSVENTIVFEDNPNCVRTAKNAGFRVAGINDRHFSEEENKMTADIADWTAWDYEYYIE
                     LFKA"
     gene            1286..1474
                     /locus_tag="PLI11_05985"
     CDS             1286..1474
                     /locus_tag="PLI11_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52448.1"
                     /translation="MQKIIDKPLFKLSIAILLIILSAYKITQGLHWDETLSIAVAILA
                     IMFSLLVQEIISNIPNIL"
     gene            1836..3203
                     /locus_tag="PLI11_05990"
     CDS             1836..3203
                     /locus_tag="PLI11_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPZ52449.1"
                     /translation="MLKKSNEIDVTKGNLIANIFIFSLPIMAMNILQLLFNAADMIVV
                     GRFAGVEPFAAVGATGPLINLIINLFMGLSVGTVVVVSQEFGAGDSKGVVESLHTSIF
                     FSIILGIFVMVLGLFIYEPLLVLMGTPEEILPLASLYIKIYFFGLPAAMVYNFCASIL
                     RAVGDSKNPMYFLVIGGIVNVILNLIFVIVFHMSVAGVAFATVISQYLSMVLILIFLY
                     RHEGLIRFKPKYLRLEWEKLVQIFRIGFPAGMQGLLFSISNTVVQSSINSFGTTTIAA
                     NTAALNVEGFVATTTNAYYNAAITFTGQSMGAKKYDKIDSVAKVCTALIFATWLILGS
                     LVIIFGRPLLSIFNSDPEVLNLGMHRIFIMMSLYFTCAFMNVFPGITRGMGYSILPML
                     CTLVGACLLRIVWIYSVFKWYPTITVLYLCYPVTWSIAGLGQVLIFFYARKRIRKKHI
                     VESTT"
     gene            complement(3248..>3822)
                     /locus_tag="PLI11_05995"
     CDS             complement(3248..>3822)
                     /locus_tag="PLI11_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPZ52450.1"
                     /translation="RMEKMKKSIWGTSLLECKYFVIHPIMPYGADKDPEPERLFEMNF
                     EFMSALLPVAKEHGVVICLENMPMTQLSLSRPAEILEFVKAINSENMQVCIDTGHCAV
                     FGESPADAVRLTGEYLKVLHVHDNDGRMDLHWLPYYGVVDWDDFNNALNEIGYDGCLS
                     LETNVSRKIPADIKEHHQIGLALIAKKLAE"
BASE COUNT         1123 a          799 c          664 g         1236 t
ORIGIN      
        1 atgtaatcag atacaatttt aacaataaca gtctgatgag gatacagaaa cttgtaagaa
       61 gcctccatta ctcctgcaga ttccatatct acaaaaacac cctcagtgtt ttcttttcta
      121 tttgttatca cattcgaaaa acaggatatg cattcttgct cgtatcctgt cacaaacaaa
      181 tcgggaaagt actccctgcc tgtatccata tctacaacct tgtttgcaag tttaagtgtt
      241 ccgagcggta cgtcaagatt gctactgccg caataaccta cgttaagaag aatatccttc
      301 tcgcacactc cgtatttgcc aaaaagataa gcaataccca tggcgcattt gactttccct
      361 acaccgctaa caatcagtat gacatcttcg tttccaaaca cttggaactc ttttgtatcc
      421 atatcctttt tcagtttgaa tccctctatt atcggagatg cttcaacaga aagagctgtg
      481 tgtatataaa tcataaacgt cccttctatg ctttaaacaa ttcaatgtaa tattcataat
      541 cccaagcagt ccagtccgct atatctgcgg tcattttgtt ctcctcttcg ctaaagtgtc
      601 tatcatttat acctgcaact ctaaaacctg cattcttggc agttctaaca caattgggat
      661 tgtcttcaaa tacaattgtg ttctctacag attgtcccag cttctctgca gcaagcaaat
      721 agatatcagg atgccttttg ctgactccaa tatcatcatt tgaaaggaca aactcaaaat
      781 attcaagaag cccaagatgc tcaagtgcga ttttcgcaac cctgccattg gtggctgttg
      841 caataccaat ccttttgcct tgtgaaagaa catgctccaa aaaattaacc gccttgtgtt
      901 ttggagtaac ctcagttgta taaaactcta tagccactct atgtaattcc tcttttatcg
      961 tatccaaatc atgatcaaaa ccatggaggt ttacataatt ctttactctt tcaaaaaaac
     1021 ttccaggttg ctcactaagc ttgatattct tgtttggttc aaagttaaat gctttggcag
     1081 catacaaatc agtattacgc caataataca atgaatccaa caacgttcca tccatgtcaa
     1141 aaaccaatgc ttctgcttta atactcgttt tcataatcat ccccttgcct tttgttacga
     1201 atatagtata gcatttttga aactgctaga caaactattg aaataagcat aatataaatg
     1261 gaaaagaaat tggaagaggt tatacatgca aaaaataatt gacaaaccct tgtttaaact
     1321 aagtattgcg atacttctca ttatacttag tgcttataaa attacccaag gacttcattg
     1381 ggatgagacg ctgagtattg cagttgcaat tcttgccatt atgttttcac tgttggttca
     1441 ggagataatt agcaacatac cgaacatttt gtaagatgaa actcatcaag agaacctgat
     1501 aataaaagca ttgtagagat tgattgtata tatcgccttt gctatagcag aatacctgcc
     1561 aatattgatt attgatagaa accctgtttt atacgtccaa ttggatttgc acaggaagat
     1621 ggtttgcatc ttttggaatg attttggcaa taacacttgc gtccaaccag agtgtagcca
     1681 taagtggtct tgcagccgta tttctgccaa cgccctcctg agtatatata cttatttatt
     1741 aattcgtaat gatgtttaaa tatttgtttg atataattac tgtacttgtg gattgtcaca
     1801 acgttttagt taaattttag gaaagtgggc gtaaaatgct aaagaaaagt aatgaaattg
     1861 atgttaccaa gggtaactta attgcaaata tttttatatt ctcattacct ataatggcga
     1921 tgaatattct gcaacttttg tttaatgctg cagacatgat tgttgtagga cgatttgcag
     1981 gagttgaacc ttttgctgca gtcggcgcaa ccggacctct cataaaccta ataataaacc
     2041 tttttatggg tttgtcggtc ggaactgttg tcgttgtatc tcaggaattt ggcgcaggag
     2101 attccaaagg cgtggttgaa tcactacata catccatatt cttcagtatc attctcggta
     2161 tttttgttat ggttctgggt ttgtttattt atgaacccct gctggtactt atgggaaccc
     2221 ctgaggagat ccttccttta gcttcattat atataaaaat atatttcttt ggtttacctg
     2281 ccgccatggt atacaacttc tgcgcatcaa tattgagagc agttggagac agcaaaaacc
     2341 ccatgtattt tctggtcata ggaggaatag taaatgtaat cctaaacctc atatttgtta
     2401 ttgttttcca catgagtgta gctggtgttg cgtttgccac cgttatttct cagtatttat
     2461 ccatggttct cattcttatt ttcctttata gacacgaagg gcttatacgc ttcaaaccga
     2521 aatacttgcg tcttgagtgg gaaaaacttg ttcagatatt caggattggt ttcccagctg
     2581 gaatgcaggg cctactgttt tctatttcca acacagtagt acagtcttca ataaactctt
     2641 ttggaacaac aaccattgca gcaaacacag ctgctcttaa cgtagaagga ttcgttgcaa
     2701 caaccaccaa cgcctattac aatgcagcaa taacatttac aggtcaaagc atgggagcaa
     2761 agaagtacga caagattgat tccgttgcca aggtatgtac tgccctaatt tttgcaacct
     2821 ggctcatact cggcagtctt gtgataatct ttggaaggcc gcttctcagc atattcaatt
     2881 cagatcctga ggtacttaat ctgggaatgc accgaatatt cataatgatg agcctttact
     2941 tcacctgcgc cttcatgaac gtttttccag gcataactcg aggtatgggc tattcaatcc
     3001 ttcctatgct ctgcaccttg gtaggtgcat gcctgctaag gatagtatgg atatattctg
     3061 ttttcaaatg gtatccaact ataactgttc tatacctatg ctatcccgta acctggtcga
     3121 tagcaggtct tgggcaggtc ttaatcttct tctatgcacg gaaacgaata agaaaaaagc
     3181 atattgtaga gtcaacaaca tagacaaaag acagactgat aaattcggtc tgtctttttt
     3241 aatcaagtta ttcagcgagt ttttttgcta tcaatgcaag tccgatttga tgatgttcct
     3301 taatatcagc aggtattttc cttgagacgt tggtttctaa cgacaagcat ccatcataac
     3361 caatttcatt caatgcattg ttgaaatcat cccagtcaac aaccccgtag tatggaagcc
     3421 agtgaagatc catcctgccg tcattatcat gcacatgtaa aaccttcaaa tactcccctg
     3481 tcaatctgac agcatcagca ggagattctc caaaaaccgc acaatgacca gtatctatac
     3541 acacctgcat gttttcactg tttatcgctt ttacaaactc cagtatttct gctggtcttg
     3601 atagcgatag ttgagtcatg ggcatgttct caagacaaat cacaacacca tgctcttttg
     3661 cgacaggaag caaagcactc atgaattcga agttcatttc aaatagtctt tcaggctcag
     3721 ggtccttatc agcgccatac ggcatgatag gatgtattac aaaatacttg cattcaagca
     3781 atgatgtgcc ccatatcgat tttttcattt tttccatacg tt
//
LOCUS       DAONVY010000191         3820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16726_length_3820_cov_3.290571, whole genome shotgun sequence.
ACCESSION   DAONVY010000191 DAONVY010000000
VERSION     DAONVY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16726_length_3820_cov_3.290571"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLI11_06000"
     CDS             <1..726
                     /locus_tag="PLI11_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPZ52451.1"
                     /translation="VIDSEDGKIKPAMVEKMCQAHYEDGDREHTVKPGMVYISNPTEL
                     GTIYLKRELEELREVCNKYNLYLFLDGARLGYGLCAEENDLTMADIASLCDVFYIGGT
                     KVGALFGEAVCITNTNLKKEFRYLIKQKGALLAKGWIMGIMFLVLFEDSLYFKIADNA
                     VKMAMRIKEAFNNKGIDFYCDSPTNQQFPILDNLQYDILKEKYLFSYIAKIDDNRHVV
                     RFCTSFATRPENVYMLVRDIEKL"
     gene            complement(727..1575)
                     /locus_tag="PLI11_06005"
     CDS             complement(727..1575)
                     /locus_tag="PLI11_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52452.1"
                     /translation="MDNRILRINFETSPDTIFIKHIKKQYPTVQPPVEHSHNRYELFA
                     ILQGVCTFFVQNTLLHMEAGDLCIVPPSCIHSISYPNTYHEHIVVNFSEQYVDKKIAD
                     ELNEVISWGKYTPTPEDTPYLVDILEDIFANSESIEEYNSLYLKTCFDRLFVKMLRTK
                     EQFNKNIKTDTLSRDISRVLEYISHNYYQDITLVEMAESCHVSPSYFSKFFKKTIGMG
                     FKEYVTFTRLTVAENLLKTTDLSIADIAYETGFNDSNYFSLVFRQHYGTPPTDYRKKR
                     RKKTTL"
     gene            1746..2045
                     /locus_tag="PLI11_06010"
     CDS             1746..2045
                     /locus_tag="PLI11_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52453.1"
                     /translation="MKRRLVLILAVMMIFSMLDVGVVQYNATGRKTVDRSDNTIVYTE
                     DFEDYANSEEMRSALGQHWTLLQPTTPDAHSLPFSYFATQLALCIVIHPIVNTAK"
     gene            2268..3452
                     /locus_tag="PLI11_06015"
     CDS             2268..3452
                     /locus_tag="PLI11_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52454.1"
                     /translation="MRKKINLLLIILIVVLSVCQLGVFGQTGNAENEPLEYFDFSIDG
                     AISGPTATNKYYSWQGENGMLRPTGTNEDKIEFVFNEEYEALQINFNGDMVTNGSCLW
                     LSLREAFEVSEGKSYYIVFRYKYLGDTEGGFLSPTQLLTGYSSVNNWSPANSGGRVLK
                     SLTAAGMPTIQDGEWLLSFARISWTDKMTSIEYDGEKTNIKVIRTSLEDKISNGSVVL
                     KSIALFEDHAGLGTMDKITEDVVKDLRTTPPVMFSMKKNFTSGEEVKYTLDDENAESV
                     TFYENVDGEWKAMEGTPQEDGEYRIEVKLKDGYWFDRAWLGTSEDVANGILVYEFTIG
                     EDVSETPEPTDEKPKETQKPDSTPKDEDGKGGNTGIIIGVVIGLVVIAAVVVVIIVRK
                     KK"
     gene            3466..>3820
                     /locus_tag="PLI11_06020"
     CDS             3466..>3820
                     /locus_tag="PLI11_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52455.1"
                     /translation="MTIRKKVLRLLFMLVCLPVFVFLVSCEGGRIAKPKDEDQRINKG
                     SRTLTGTSDTGVKVSMYGELETDKQVGMFYFIWHGTGLGSSYGTNYDNSKILKNIDFS
                     GGRKSINPEEWLEAGG"
BASE COUNT         1180 a          602 c          882 g         1156 t
ORIGIN      
        1 gttattgact cggaagatgg aaagataaaa cctgcaatgg tagagaaaat gtgtcaagct
       61 cattatgagg atggtgacag ggaacatact gtaaagcctg gaatggtgta tatatcgaat
      121 cctacggaac tgggtacgat ttatttgaag agggaactgg aagaactaag ggaggtatgc
      181 aacaagtaca acctttattt attcctggac ggagctaggc ttggatatgg cttgtgtgct
      241 gaggaaaacg atttgaccat ggcagacatt gcttccttgt gcgacgtctt ttatattggc
      301 gggacgaagg taggagcttt gttcggagag gcagtttgca ttacaaatac aaatcttaag
      361 aaagaattca ggtatctgat aaagcaaaag ggagctctct tggcaaaggg gtggattatg
      421 ggcattatgt ttcttgtcct ttttgaggac agcctgtatt ttaaaatcgc tgacaatgca
      481 gtcaaaatgg caatgagaat caaagaagca tttaacaaca aagggattga cttttactgc
      541 gattctccga caaaccagca gttcccgata cttgataatt tacagtatga catactgaaa
      601 gagaaatatc ttttcagcta tattgcaaaa atagatgaca acagacatgt ggtgcgcttt
      661 tgcaccagct ttgctacaag accggaaaat gtatatatgt tagtaagaga tatagagaag
      721 ttgtgattaa agcgtggttt ttttccttct tttctttctg taatccgtag ggggagtacc
      781 atagtgctgt ctgaaaacca gggagaaata attgctgtca ttgaagcctg tttcataggc
      841 aatgtcagct atcgacaaat ccgtggtttt cagaagattc tccgctacgg taagtcttgt
      901 aaaagtcaca tactctttga atcccatacc aattgttttt ttgaagaatt ttgagaagta
      961 tgagggtgat acatggcagg attcggccat ttccaccaag gttatatctt gataataatt
     1021 gtggcttatg tattccaata ctcttgaaat atctcttgaa agtgtgtctg tttttatgtt
     1081 tttgttgaac tgctcttttg ttctcagcat tttgacaaaa agtctgtcaa agcatgtttt
     1141 taagtaaaga gaattgtatt cttctatgct ttcggaattt gcaaaaatat cttcaagaat
     1201 atccactaag tatggggtat cttcaggagt aggcgtatat tttccccagc tgatgacttc
     1261 attaagttca tctgcaatct tcttatccac atactgctct gaaaaattga ccactatgtg
     1321 ttcgtgatat gtatttggat atgagatgga gtgtatgcag ctaggaggaa ctatgcataa
     1381 gtctccagct tccatgtgca aaagcgtgtt ttgtacgaag aatgtacata ctccctgcaa
     1441 tatggcaaac agctcgtatc tgttgtgcga gtgttctaca ggaggctgta cggtcgggta
     1501 ttgctttttt atgtgtttta taaagatagt gtcaggtgaa gtttcaaaat taattcttag
     1561 tattctgtta tccattatat ctgtcctctc cgattttata attgcattat acagaaatag
     1621 agcaaaaaat tcaagatgag tatacttttt tgacaaaaat attatggagt tctttaaatt
     1681 aatgtggttt actagtatca gatacctgca tgaggataga attattttag ggaggatgtt
     1741 atattatgaa aaggagatta gtattaatac ttgctgttat gatgattttc tccatgttgg
     1801 atgttggggt tgtccaatat aacgcaacag gtagaaaaac tgtcgacaga agtgacaaca
     1861 caatagtata cacagaggat tttgaagact atgctaattc agaagagatg aggtcagcac
     1921 ttgggcagca ttggactttg cttcagccaa ccacgccaga tgctcacagt ttaccattca
     1981 gctacttcgc cacccaactg gctctttgca ttgtcatcca tcctattgta aacactgcga
     2041 agtgaatctt ctttggtttc accatagaaa tagtatgtat tgccatcctc atcaacacac
     2101 tcaagataaa ctcttacatc aatgattgag gaggtttata caatgaagat gtattatgaa
     2161 gagcctgatt ttgatataat tcagtttgaa gttgaggata tgattacttt aagtggtact
     2221 gatttcatgg gtattgattt tgaggatttg atttaagggg tgaaaatatg agaaagaaga
     2281 taaatttatt gctaataata ttgatagttg ttctttctgt ttgccagctt ggtgtttttg
     2341 gtcagacagg aaatgctgaa aacgaacctc tcgaatattt tgatttttca atagatggag
     2401 cgatttcagg gccaactgca acaaacaagt attatagctg gcagggcgaa aacggaatgc
     2461 tcaggccaac tggaacaaac gaagacaaga tagagtttgt attcaacgaa gagtacgagg
     2521 ctttgcagat aaactttaat ggcgacatgg ttactaacgg ttcttgtctg tggttaagtc
     2581 tcagagaagc ttttgaggta agtgaaggca agtcatacta tattgtattc aggtacaagt
     2641 atctgggcga tactgaaggt ggattcttgt cacccacaca gctgctaacg ggatattctt
     2701 ctgttaacaa ctggtctccg gcaaactcag gtggaagggt gctgaagtca ttaacagctg
     2761 caggaatgcc tacaatccag gatggcgaat ggctccttag ctttgcaagg atttcctgga
     2821 ctgataaaat gacttctata gagtatgatg gagaaaagac gaatatcaag gttatacgta
     2881 cttctcttga ggataaaata tcgaacggat ccgttgtatt gaagagtatt gcattgtttg
     2941 aggaccatgc tggactagga accatggata aaataactga agatgtggta aaagacttaa
     3001 gaaccactcc tcctgttatg ttctcgatga agaagaactt tacatccgga gaggaagtta
     3061 aatacacttt ggatgatgaa aatgctgaaa gcgtaacctt ctatgagaat gttgatggtg
     3121 agtggaaagc catggagggt acccctcagg aagatggaga atacagaatt gaagttaagt
     3181 taaaggacgg ttactggttt gatagagcat ggcttggaac gtccgaagat gtggcaaatg
     3241 gtattcttgt ctatgagttt acaataggtg aagatgtttc tgaaactcca gagcctactg
     3301 acgagaaacc taaagaaaca caaaagcctg actcaacacc caaggatgag gatggcaagg
     3361 gtggcaacac aggtattatt ataggtgttg ttattggttt ggttgttatt gcagcagtcg
     3421 ttgtagttat aattgtacgc aagaaaaaat agaaaggtag aaaagatgac aattagaaaa
     3481 aaggttttga gactattatt catgcttgtg tgtcttcctg tatttgtttt tcttgtttcc
     3541 tgtgagggtg gaagaatagc aaaacccaag gacgaagacc agaggataaa caagggtagc
     3601 agaaccctca caggaacgtc tgatacagga gttaaggttt ccatgtatgg agaactggaa
     3661 accgacaaac aagttggcat gttctatttc atctggcatg gtacaggact tggttcttcc
     3721 tacggaacca attatgacaa tagtaagata ctgaagaata ttgatttttc aggtggacgg
     3781 aaaagtataa accctgaaga atggcttgaa gcaggaggcg
//
LOCUS       DAONVY010000192         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16840_length_3799_cov_3.235043, whole genome shotgun sequence.
ACCESSION   DAONVY010000192 DAONVY010000000
VERSION     DAONVY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16840_length_3799_cov_3.235043"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1044
                     /gene="pdxA"
                     /locus_tag="PLI11_06025"
     CDS             100..1044
                     /gene="pdxA"
                     /locus_tag="PLI11_06025"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPZ52456.1"
                     /translation="MAKPAVAITMGDPLGIGPEIVIKSMAHIEVFEVCKPIIVGSRSI
                     LKKAAELQNLVETYEKVEESIVDVYTEENITQKAAGKAAFEYINKAVSLCMDKRTDAI
                     ATAPINKEHLKEANIPYIGHTEILAGLTNTDDPLTMFETDSLRIFFLSRHVSLIEACN
                     LVKKERIYQYISRCDNALKKLGVDKGTMAVAALNPHSGEGGMFGREEIEEIIPAVNMA
                     KTDGIDVVGPISADSVFHLAHIGRYNSVLSMYHDQGHIASKTMDFEGTISLTIGLPFL
                     RTSVDHGTAMDIAWKGIANERSMVEAILVAAKYAPKYN"
     gene            complement(1090..1794)
                     /locus_tag="PLI11_06030"
     CDS             complement(1090..1794)
                     /locus_tag="PLI11_06030"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPZ52457.1"
                     /translation="MTESNFSRQMMDFFEGLLQINGIENVLRAAMTVLVASVFGLLIY
                     FTYKLTCRPFDYQREFGLVLIVVPIVVSILLTVIGTNIARAFSLAGALSIIRYRSTLV
                     KPKEIVFIFFGMGTGFITGCFLYLPALVFVLITCVVIIVYTAFTLERGRNVAKTLTIA
                     VPENINYDGLFDEILEKFTSSYALNSIRIISGGAVTELQYHIKIKDISMTKELLDEVR
                     VLNQNFKIQLSQFATQ"
     gene            complement(1862..2638)
                     /locus_tag="PLI11_06035"
     CDS             complement(1862..2638)
                     /locus_tag="PLI11_06035"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPZ52458.1"
                     /translation="MIKLFIENFSRHEYKFPMTYDNMDRLLDDLLPYIEQDEHADENG
                     YYTISSIYLDNDTWQCFYETINKDRYRQKVRLRVYGEVNNDSVSFFEIKSKFKGLVLK
                     RRVKMRLGDAMRFMEACVNGEDPNVDEYECSNRQILKELKQVIVSKNLKPVVVVSYER
                     LALFYKEDPRLRITFDVNIRTRDYDIDLTKGSHGDYVTADNVAILEVKSDRNVPFWLV
                     RILSKYGYRNQTFSKYCSHFIKYSEEEQIEEYDELDNDAV"
     gene            complement(2732..>3799)
                     /locus_tag="PLI11_06040"
     CDS             complement(2732..>3799)
                     /locus_tag="PLI11_06040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPZ52459.1"
                     /translation="VQKYPVDSIPEEKNPELEDRTFTRHRAFVSDLKPGTRYLYRLGE
                     GDNLSDVYTFKTPEQGKDFSFIIVADSQGYKRSDFEHYKRVLDKANDMFGDVDFYIHL
                     GDFVDDGKNFLQWKYVLDIPRDIMANNTFVPVAGNRDDMEVFEYFTLGSVYDTRPMAN
                     GYYSFSFNDVHFVVLYTAGNKSLPKKQMSFLEDELKKHKDSRVVVLTHKAPYTNANHA
                     DDKEIVALREDLLPVFDKYNVDLVLQGHDHFFFRSEPIHEGKVGNYTYEKVMDGDDDV
                     DMMSDVKGTIYLINSSSGVSQYDGAFRNMDDIHAFKSFNMNNPTFTYCAVTENGIILK
                     TYEVNVMRQRVQLIDSFGIRS"
BASE COUNT         1221 a          798 c          655 g         1125 t
ORIGIN      
        1 ttatcagatt gttaaaacat agttcatata atatctgtct gacactggca actgaaatat
       61 gctaaaataa atcaaacgaa atatgaaagg atgatttaat tggcaaagcc tgccgtagca
      121 ataacaatgg gcgatccgct gggaatcgga ccggaaattg taatcaaatc aatggctcac
      181 attgaagtat tcgaagtatg taaaccgata attgtaggaa gccgcagtat actcaaaaaa
      241 gcggctgaac tgcaaaacct tgttgaaaca tacgaaaaag tggaagaatc aattgtagac
      301 gtatacacag aagaaaacat aacacagaaa gcagctggaa aagcggcttt tgaatatatt
      361 aacaaggcgg tttcgctatg catggacaaa agaacagatg caattgcaac cgctccaata
      421 aacaaggaac accttaaaga ggcaaacatc ccatatatcg gtcacacgga gatattggca
      481 ggacttacga acacggacga ccctcttaca atgtttgaaa cagactccct aaggatattc
      541 ttcctgtcaa gacatgtgtc gttgattgaa gcatgcaatc ttgtaaaaaa ggaacgcatc
      601 tatcaatata tttcaagatg tgacaatgca cttaaaaaac tgggagttga caagggtaca
      661 atggcagtgg cagccttaaa tccccacagt ggtgaaggag gaatgtttgg aagagaagaa
      721 atagaagaga taattcctgc tgtaaacatg gccaaaaccg atggcataga tgtagtcggt
      781 ccaataagtg cagactctgt ttttcatctt gcccatatcg gaagatacaa ctccgttcta
      841 tccatgtacc acgatcaggg acacattgcc tcgaaaacaa tggactttga gggtaccatt
      901 tccctgacaa taggactccc cttcctcagg acatcagtag atcatggcac cgccatggac
      961 atagcctgga aaggcatagc taatgaaaga agtatggttg aggcaattct tgtagcagca
     1021 aaatatgccc cgaaatacaa ttaatggacg agtgcaatac ccgtccatta aatatattca
     1081 taagatatat tattgagttg caaactgtga caactgaatc ttgaagttct gattcaatac
     1141 cctcacttca tcaagaagct cttttgtcat cgaaatgtct tttatcttga tatggtattg
     1201 cagttcagtg acagctccgc cgctgattat gcgaatgctg ttaagtgcat aagaacttgt
     1261 gaacttctcg agtatttcat caaacaaccc atcgtaattg atattctcag gcacggcaat
     1321 tgtaagggtt tttgcaacgt ttctgcctct ttcaagagta aatgcagtgt atacgatgat
     1381 aacaacgcaa gtaataagca cgaataccaa tgcgggcaga tacaagaaac aacctgttat
     1441 gaaacctgtg cccatgccaa agaatataaa tactatttct tttggcttga ccagagtact
     1501 tctgtatctg ataattgata acgcacctgc aaggctaaaa gcacgagcta tgttggttcc
     1561 gattacagta agtagtatgg acacaactat gggaacaact atcaatacaa gaccaaactc
     1621 tctctggtaa tcgaaaggtc tgcatgtaag cttgtaggta aagtagataa gtaacccaaa
     1681 tacacttgcc accaaaacag tcatagctgc tctcagcaca ttttcaatac cattaatctg
     1741 caataaacct tcaaaaaaat ccatcatttg tcgagaaaaa ttgctttctg tcatttcttt
     1801 gtctcctatc tatttttgaa tattttttat ttattaaaaa acaaaattat gaccaacctc
     1861 attaaaccgc atcattgtcc agctcatcgt attcttcaat ttgttcttct tcactgtact
     1921 ttatgaaatg cgagcaatac tttgaaaaag tctgatttcg ataaccgtac ttcgaaagaa
     1981 tccttacaag ccagaaagga acgtttctgt cggactttac ctctagaatt gcaacattat
     2041 ccgctgtcac ataatctccg tgggaacctt ttgtcagatc aatatcataa tctcttgttc
     2101 tgatatttac atcaaatgtg attctaagac gtggatcctc cttgtagaac aaggccagtc
     2161 tttcataact cacaacaaca acaggtttaa ggttcttgga aacaataact tgtttcaatt
     2221 ctttaagaat ctgcctgttt gaacactcat actcatcaac gttcggatct tctccattta
     2281 cacatgcttc cataaatctc atggcatcac caagacgcat tttgacgcgt cgctttaaaa
     2341 caagaccttt aaattttgat ttaatctcaa aaaaagagac actgtcgttg ttcacttcgc
     2401 catatacgcg cagtcgaact ttttgtctgt atctgtcttt gttgatagtc tcgtagaaac
     2461 actgccatgt atcgttgtcc aaatagatgc tgctgattgt atagtatcca ttttcatctg
     2521 catgttcatc ctgttctatg tagggaagca aatcgtcaag caacctatcc atgttgtcat
     2581 atgtcatagg aaacttgtac tcatgtctgc taaagttctc aataaacagt tttatcacct
     2641 cgatatataa cgctaacact ttcttaatac attaacgcta ttgattatac tatttgatat
     2701 aacaaatata aagtgttaaa ttataaactt tttatgaacg aatgccaaag gaatcaataa
     2761 gctgcaccct ttgacgcatt acgtttactt catacgtttt taatattata ccattttcag
     2821 ttacagcaca ataagtgaag gttggattat tcatgttgaa tgacttgaaa gcatgaatat
     2881 catccatatt tcggaaagct ccgtcatatt gactgacacc ggaactgctg tttatcaaat
     2941 atatagtacc ttttacatca ctcatcatgt caacatcgtc atctccatcc ataacttttt
     3001 catatgtata atttcctacc ttgccctcat gaatgggttc tgatctgaaa aagaaatgat
     3061 catgaccctg cagcacaaga tccacattat acttgtcaaa cacgggcaaa aggtcttccc
     3121 gcagtgctac aatttccttg tcatctgcgt gattcgcatt agtataaggt gctttatgag
     3181 taagtactac tactcttgaa tccttgtgct ttttaagctc atcttcaaga aagctcatct
     3241 gttttttagg cagactcttg tttcctgcgg tatacaaaac aacgaaatga acatcattaa
     3301 atgaaaaaga ataataaccg ttggccatgg gtcttgtatc ataaacactt ccaagggtaa
     3361 aatactcaaa aacctccatg tcgtcacggt tgccggccac aggaacgaaa gtattgtttg
     3421 ccattatatc ccttggaata tccaaaacgt atttccactg aagaaaattc tttccatcat
     3481 caacaaaatc cccaagatgt atgtagaaat ccacatctcc aaacatatcg ttagctttat
     3541 caagcaccct tttgtaatgc tcgaaatcgc ttctcttgta tccttgcgaa tccgcaacaa
     3601 ttatgaatga aaaatccttg ccctgttcag gtgtcttgaa ggtataaaca tcgcttagat
     3661 tgtctccttc ccccaacctg tacaaatacc tggtgccagg ctttaaatct gaaacaaacg
     3721 ccctgtgacg tgtaaatgtt ctgtcttcca attcaggatt cttttcttct ggtatgctgt
     3781 caactggata cttctgcac
//
LOCUS       DAONVY010000193         3797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16847_length_3797_cov_3.236237, whole genome shotgun sequence.
ACCESSION   DAONVY010000193 DAONVY010000000
VERSION     DAONVY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16847_length_3797_cov_3.236237"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PLI11_06045"
     CDS             complement(<1..1673)
                     /locus_tag="PLI11_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPZ52460.1"
                     /translation="MKMVNITIDGMNMQVPEGTTILEAARQANIRIPTLCFLKDINEI
                     GACRMCVVEVKGARALQAACVYPVAEGLEIKTNSDKVRRARKVTLELILSNHDRECLT
                     CVRSNNCELQKLSKELNIRDIRYKGEQTEHDIDNLSPSIVRDNNKCILCRRCVSVCQN
                     VQTVSVLETVSRGFDTKIGTAFDIPLANTPCVNCGQCINVCPVGAIYEKSDIEKVWSA
                     INDPDKYVVVQTAPAVRVSIGEAFGMPLGSRPTGKMVSALKKLGFDKVFDTDTAADLT
                     IMEEGTELLQRMQNGGKLPLITSCSPGWIKFCEHNYPDFLDNLSSCKSPHEMFGAVLK
                     TYYAKKMNIEPEKIVVVSIMPCTAKKFEAGRPELSAAGYPDVDYVLTTRELAQMIDEA
                     GIDFVNLEDETFDDPIGDASGAGVIFGATGGVMEAALRTVADILSGESLPEDKIEYTE
                     VRGVQGIKEAQIEVAGKVIRACVVHGLGNARKVLDRIRAGEAEYDFIEIMACPGGCVN
                     GGGQIIQPSEVRSWIDLRVERTKAIYAEDRSLPIRKSHDNPFIKKLYGE"
     gene            complement(1695..3485)
                     /locus_tag="PLI11_06050"
     CDS             complement(1695..3485)
                     /locus_tag="PLI11_06050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPZ52461.1"
                     /translation="MQIYRAHVLICGGTGCTSSGSIGLMDEFEKCIKEANIENEIKIV
                     KTGCFGLCAEGPIVVIYPEGCMYCRVTKEDIKEIVEEHLVKGRIVKRLLEGADAPEVD
                     TSAEQTDFFKKQYRIALRNCGKINPESIEEYIAEEGYMALGKVLTEMTPEQVIQTILD
                     SELRGRGGAGFPTGRKWEFARRSVSDQKFVCCNADEGDPGAFMDRSILEGDPHVVLEA
                     MAIAGYAIGADKGYIYVRAEYPIAVQRLKTAIKQAKEYGLLGDNIFGTDFSFDIELVL
                     GAGAFVCGEETALMTSIEGMRGEPRPRPPFPAVKGLWQQPTILNNVETYANIPVIINN
                     GPEWFKQIGTEKSRGTKVFAVGGKIKNTGLVEIPMGTTLREVVYEIGGGIPGDKKFKA
                     AQTGGPSGGCIPASLIDTPIDYDSLIKIGSMMGSGGLIVMDEDTCMVDIAKFFLEFTV
                     EESCGKCPPCRIGTKRMYDILEKITNGNGELSDIDDLYELAEGIQSSALCGLGQTSPN
                     PILSTLQYFKDEYIAHVVEKRCPAGVCKSLLRYRIIADKCRGCSLCARHCPTNCISGE
                     IKKPYVIDESKCIKCGVCMEKCKFDAVVRE"
     gene            complement(3507..>3797)
                     /locus_tag="PLI11_06055"
     CDS             complement(3507..>3797)
                     /locus_tag="PLI11_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPZ52462.1"
                     /translation="FRIVVGMATCGIAAGARPVMHAFVEELNKRKLTNAAVTMTGCMG
                     MCRLEPIVEVIDQEGNKVTYVKMTPEKAVKVVTDHIVNGKIVTEYTVGAVTD"
BASE COUNT          950 a          991 c          674 g         1182 t
ORIGIN      
        1 tactcgccgt agagtttctt gataaacgga ttatcatggg actttctgat tggcagagac
       61 ctgtcttcag cgtaaatcgc cttggttctt tcaactctaa ggtcaatcca gctcctaact
      121 tctgatggct ggattatctg tccaccacca tttacgcatc cgcctgggca tgccatgatt
      181 tcaataaaat catactcagc ctcgcctgca cggattctgt caagaacctt cctcgcattt
      241 ccaagaccgt gaactacaca agctcttatc actttgccgg caacttcaat ctgagcttct
      301 ttgattcctt gaacacctct tacctcggtg tactcaatct tgtcctcagg cagcgactct
      361 ccacttagga tatcggcaac tgtcctaaga gcagcctcca taacaccgcc tgttgcgccg
      421 aagatgactc ccgcaccgga tgcatctcca ataggatcgt cgaaggtttc gtcttcgagg
      481 ttaacaaaat ctataccagc ttcgtcaatc atttgagcaa gctctcttgt agtcagtaca
      541 taatcaacat caggatatcc tgctgctgaa agctcaggtc tgcccgcctc aaacttcttg
      601 gcagtgcatg gcatgattga tacaactaca atcttttccg gttctatgtt cattttcttt
      661 gcatagtatg tcttaagcac tgcaccaaac atttcatgtg gtgatttgca gcttgacaaa
      721 ttgtccagga aatcaggata gttgtgctcg cagaacttaa tccatccagg actgcaggat
      781 gtaatgagcg gcagtttacc gccattctgc attctctgca gaagctctgt accttcctcc
      841 attattgtca ggtcggcagc tgtatcagta tcaaatacct tgtcaaaacc aagcttctta
      901 agcgcgctaa ccattttgcc tgttggacgt gaacctaatg gcattccaaa agcttcacct
      961 attgatactc tgacagcagg agcagtctga acaacaacat acttgtcagg gtcgttgatt
     1021 gcagaccaaa ccttttcgat gtcgctcttc tcatagattg caccaaccgg gcatacattt
     1081 atgcactgtc cgcagtttac gcatggagtg ttggcaagag gtatgtcaaa cgcagtacca
     1141 atcttggtat cgaaacctct gctgacagtc tctaaaacgc ttacagtctg aacattctga
     1201 caaacactta cacatcttct gcaaagtatg catttgttgt tgtcccttac aattgaaggt
     1261 gacaaattat ctatatcatg ttctgtctgt tcgcccttat agcgtatatc tcttatgttc
     1321 aattcctttg acaacttttg aagctcgcaa ttgttagacc ttacacaagt gaggcactcc
     1381 ctgtcatgat ttgagagtat gagctccaaa gtaacttttc tggctcttct taccttgtcg
     1441 gagttggtct ttatttcaag accttccgct acaggataaa cacatgcagc ctgaagagcc
     1501 cttgcacctt taacttccac aacgcacatt ctgcaggcac caatttcatt gatgtccttc
     1561 aggaagcaca gcgttgggat gcgtatattt gcttgtctgg cagcttccag tatggtagtg
     1621 ccttcaggca cctgcatatt catgccatct atcgttatat taaccatttt catagtgtaa
     1681 tcctctttcc tcctttactc tctgacaacc gcatcgaatt tgcatttctc catgcaaact
     1741 ccgcacttta tgcatttgct ttcgtctatt acataaggct tcttgatttc tccgcttatg
     1801 caatttgtgg gacaatgtct tgcacaaagg ctacatcctc tgcacttatc cgcaatgatt
     1861 ctgtagcgaa gcagtgactt gcatacgcct gcaggacatc tcttttcaac aacgtgagca
     1921 atatattcgt ccttgaaata ttgcagtgtg ctaagaatcg gatttggcga agtttgtcct
     1981 aggccgcaca gagcacttga ctgtatgcct tctgccagct catacaaatc atctatatca
     2041 gaaagctcac catttccatt tgtaatcttt tcaagaatgt catacattct ctttgttccg
     2101 attctgcatg gcgggcattt tccgcaggac tcttcaacag taaactcaag gaaaaacttt
     2161 gcaatgtcaa ccatgcatgt atcctcgtcc attactataa gtccacccga ccccatcatt
     2221 gaaccaatct ttatcagtga gtcataatcg attggtgtgt cgatcaagct tgcaggtatg
     2281 catccgcctg atggaccgcc ggtttgagcc gccttgaact tcttgtctcc tgggattcct
     2341 ccaccaattt catatacaac ttctctcaaa gttgtaccca ttggtatttc aacaagacct
     2401 gtattcttga tctttccacc aacagcaaat acttttgtac ctctgctctt ttcagttcca
     2461 atctgcttga accactccgg tccattgttg attattacag gaatgtttgc ataagtttca
     2521 acgttgttga gaattgtagg ctgctgccac aaacctttaa ccgctgggaa aggaggtctt
     2581 ggtcttggct ctccacgcat tccttcaata cttgtcatca aggcagtttc ctcaccacaa
     2641 acaaatgcgc cagcgccaag tacaagctct atatcaaatg aaaaatcggt tccaaaaatg
     2701 ttgtctccaa gcaaaccata ctctttagcc tgcttgatag ctgtcttgag tctttgaacc
     2761 gctattggat attcggctct aacataaata tatcccttgt cagctcctat ggcataccct
     2821 gctattgcca tagcttcgag tactacatgt gggtcgcctt caaggatact tctatccata
     2881 aatgctccag ggtcgccttc gtcggcgttg cagcatacaa atttttggtc tgatacgctt
     2941 ctgcgtgcaa actcccattt tcttcctgtt gggaaacctg ctccacccct gcctctcaat
     3001 tctgaatcca gaatcgtctg aataacctgt tcaggggtca tttcagtaag tacctttcca
     3061 agagccatat aaccttcttc agctatgtat tcttcaatgg actccggatt tatcttaccg
     3121 caatttctaa gagctattct atactgtttc ttgaagaaat ccgtttgttc agcacttgta
     3181 tcaacttctg gagcatcagc accttctaga agtctcttaa caatacgacc ttttacaaga
     3241 tgttcctcaa ctatctcctt gatatcttcc ttggttaccc tgcagtacat gcaaccttca
     3301 ggatatatta ccactatggg tccttccgca cacagaccaa agcaacctgt cttgacaatc
     3361 tttatctcat tttctatgtt ggcttccttg atgcactttt caaattcatc catcaatcct
     3421 atggaacctg atgaagtgca gccggttcca ccacaaatga gcacatgtgc tctatatatc
     3481 tgcattactt atcctccttc aatagattaa tcggtaactg caccgacagt atactcggtg
     3541 acaatctttc catttactat atgatcagta acaaccttga cagccttctc aggtgtcatc
     3601 ttaacgtatg ttactttgtt gccttcctga tctattacct caacaatggg ctccaaacgg
     3661 cacataccca tgcagccagt cattgttacg gcagcatttg tcagctttct cttgtttaat
     3721 tcttctacaa aagcgtgcat tacaggacgc gcgccagcag ctattccaca tgtggccata
     3781 ccaaccacta tcctaaa
//
LOCUS       DAONVY010000194         3779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16926_length_3779_cov_4.320892, whole genome shotgun sequence.
ACCESSION   DAONVY010000194 DAONVY010000000
VERSION     DAONVY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16926_length_3779_cov_4.320892"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..484
                     /locus_tag="PLI11_06060"
     CDS             20..484
                     /locus_tag="PLI11_06060"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HPZ52463.1"
                     /translation="MKKHGYYRRNYFSKTFSGKVLIRRYLCSKCKKTLSFLPSFCQPF
                     FQYSPGDFMDILHQSFSRDTSLDVFCSEIRNKFPNLSFFRQHIRFYLNRIAKKLNTIK
                     MYLRDINIKVKLPWSEAASGEKVRDVLDNIKAFCSFSEFITKQNKAILSFSF"
     gene            644..1114
                     /locus_tag="PLI11_06065"
     CDS             644..1114
                     /locus_tag="PLI11_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPZ52464.1"
                     /translation="MDRTLDDKTREAVALKKFSLIGPVLNGLVKNQNEYFRKLSNKVI
                     QMPYYGPKQYSFKTFKGWVYDYRRGGIDALKPGYRKDRGSSRKVSGALSSVICKRIKE
                     NERMPLKLIYEKMVEDDEINPEKVSLSTFYRHVSELQKTCRQGQVKMIKNRKEY"
     gene            1423..2019
                     /locus_tag="PLI11_06070"
     CDS             1423..2019
                     /locus_tag="PLI11_06070"
                     /inference="COORDINATES: protein motif:HMM:NF020859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HPZ52465.1"
                     /translation="MVPRGRGKIERYFRTVRTRFLSRINSIKLKHVDELNEKYFYWLE
                     HDYQNKKHSSLGISPLDFFLKQADDIRLPSNPQWLEEVFLIRKKRTVNHDATIQIENI
                     LYETQPHLKGMRVEIRYEPEWLLGSDKPVLIYVDGKKAGDARRVDFISNVYVKRSKSI
                     KKRVDASEGEADCNDEELSKEEYTQSISFTSMMEGSEK"
     gene            2019..2816
                     /locus_tag="PLI11_06075"
     CDS             2019..2816
                     /locus_tag="PLI11_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ52466.1"
                     /translation="MFTQYFGMKFNPFSKEIPSDNLYESNDFKELESRLKYLQKTRGI
                     GLVIGEPGTGKSTALRRYVSSLNRSLYKPCYMALSTLTVMDFYHSLAQMLGEVPAHRK
                     IKMFNQIQDAINSLYYDQKITPILIMDEIHMATSGILEDLRLLLNFRMDSANPYILIL
                     CGQPPIRNRLAMNANLPLRQRISVRYMMKGLNKDEVPEYIKTRLKAAGTREDIFLPET
                     MKLIHANTGGFTRPINNLATASLMYAASVKKDYVDEEVIFQAQKELN"
     gene            2819..3355
                     /locus_tag="PLI11_06080"
     CDS             2819..3355
                     /locus_tag="PLI11_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04540 family protein"
                     /protein_id="HPZ52467.1"
                     /translation="MKSIKRDCIEGIYRDGHGAITRIIVGKYRKGVFFCIPKFKTGIQ
                     IDQNCSVDSITNILKKTVLGSHTKNIEGIAKKTIEIINELSDLTSNEDPAKNIIKNPS
                     TVKQMAEQIIVAADAYFSLRLEEDEFQELILNYASRHGKKLFGKNGKINPTIRNIIGK
                     KRRELVELMLRGYQLSVF"
BASE COUNT         1310 a          638 c          777 g         1054 t
ORIGIN      
        1 ctgtggaaag tattgcagga tgaaaaaaca tggatactat cgtaggaact atttcagtaa
       61 aacattttca ggaaaagtcc tcataagacg ttatctatgc agcaagtgca aaaagacgct
      121 atcatttctc ccaagctttt gccaaccgtt cttccaatat agcccgggag atttcatgga
      181 tatattgcat caatcattca gtcgggatac cagccttgat gtattctgca gtgagataag
      241 aaacaagttt ccaaaccttt cttttttcag gcagcacatt agattctatc ttaacaggat
      301 tgcaaagaaa cttaatacta ttaaaatgta ccttcgtgat atcaatataa aggtaaagct
      361 gccttggtcg gaggcagctt caggtgaaaa ggtcagagat gttttagata acatcaaagc
      421 tttttgttct ttctcagaat tcataaccaa acaaaacaaa gcgatactgt ccttttcatt
      481 ttaatgataa catttttgga tatgaaaatg gaagagtttt tgttgatttt tagcagcaac
      541 acaacttttc cgctgacttg ttgacaaacc tggtataaaa tgtaccacag gaacaataaa
      601 accggttttg ctgctcctta aataaatttt aggggtgtgt ataatggata gaacacttga
      661 tgataagaca agggaagcag tagccttaaa aaaattttca ttaattggac ctgtgttaaa
      721 tggtcttgtc aaaaatcaga atgaatattt cagaaaacta agcaacaagg ttatacaaat
      781 gccttactac ggaccaaaac aatattcatt caagactttt aaagggtggg tctatgatta
      841 ccggcgagga ggaatagatg ccttgaaacc agggtatcga aaagacagag gttcatccag
      901 aaaagtcagc ggggcgcttt ctagtgtaat ttgtaaaagg attaaagaga atgaacgtat
      961 gccattaaag ctaatttatg agaaaatggt ggaagatgac gaaatcaacc ctgaaaaggt
     1021 ttctctttca acattctacc ggcatgtgtc agaactgcaa aaaacatgcc gtcagggtca
     1081 ggtcaaaatg ataaagaacc ggaaagaata ctgagatatt cctttgaaaa aatcaatgcc
     1141 tgttggcaaa ttgacatcat gtatgggcca tatatacatt acgggaaaag caaaagacag
     1201 acatatctgg tcggtataat tgatgatgct agcaggttaa tagtacattc tgccttttat
     1261 cataatcaga agtatgaatc acttcgacat tgtcttaagg aggccgtatc aaggcgaggt
     1321 atccctggta tagtctatac agacaacgga aaaattttca ggtcccagca atttgaatat
     1381 ctgtgtgcgg agataggtag ttctcttatt cattcacaac ccatggttcc ccgtggtcgc
     1441 gggaaaattg agaggtattt caggacagtg cgcacaaggt tcttaagcag gatcaacagt
     1501 attaaactaa agcatgttga tgaactcaac gaaaagtatt tttactggtt agaacatgat
     1561 taccagaata aaaaacattc ctcgcttggc atcagtcctc ttgacttttt tcttaaacaa
     1621 gcggatgata tcaggcttcc aagtaatcca caatggcttg aagaagtctt cctgataaga
     1681 aagaagagaa cagtcaatca tgatgcaacc atacagattg agaatatact ttatgaaaca
     1741 cagccgcacc ttaagggaat gagggtggaa atcaggtatg aacctgaatg gcttttgggg
     1801 tcagacaagc ctgttctgat atatgtcgat ggcaaaaaag ccggggacgc acgcagggtc
     1861 gattttatat ccaacgttta tgtaaagcgc agcaaaagta ttaaaaaacg tgtagacgct
     1921 tcagaaggtg aagctgattg taatgatgag gaattatcaa aagaagaata cacacaatcg
     1981 atatcattta ccagcatgat ggaaggaagt gagaagtgat gtttacacaa tattttggta
     2041 tgaagtttaa tccgttcagt aaggaaattc cttctgacaa tctatacgag agcaacgatt
     2101 ttaaggaact tgaatcaaga ctcaaatatc tgcagaaaac acgtgggata ggtcttgtca
     2161 tcggagagcc cggcaccggc aaatctactg ctttaagaag gtatgtaagt agtcttaaca
     2221 ggtctttata caagccttgt tacatggcat tgtcaacatt aaccgttatg gatttttatc
     2281 acagccttgc gcagatgctc ggtgaagtac ccgcacaccg taaaatcaag atgtttaacc
     2341 agatacagga tgcaataaat tcattgtact acgaccagaa gataacacca atactgatca
     2401 tggatgaaat ccatatggcg acaagcggta tccttgaaga tttaaggctt ttgttgaatt
     2461 ttcggatgga ctcagcaaat ccatacatat taatactttg cggccaacca cctatacgca
     2521 atagactggc tatgaatgcc aaccttccgt taaggcagag aatttcagta agatatatga
     2581 tgaagggact gaataaagat gaggtacccg aatatattaa gacccgtctc aaggcagcag
     2641 gtaccagaga agatatattt cttcctgaaa caatgaaact tattcacgca aatactggtg
     2701 gatttacacg tccgatcaat aatcttgcaa cagcttccct catgtatgca gcctcggtca
     2761 aaaaagatta tgttgatgaa gaagtaatat ttcaggcaca aaaggagttg aattgagtat
     2821 gaaaagcatc aaaagagatt gtattgaggg tatatacagg gatggacatg gagctattac
     2881 cagaattatc gttggtaaat accgtaaggg tgtgtttttt tgcatcccaa agtttaaaac
     2941 aggaatacag atcgatcaga actgctcggt agatagcatt actaatattt taaaaaaaac
     3001 tgtcttgggt tcccatacta aaaacataga gggcattgcc aaaaaaacta ttgaaatcat
     3061 taacgagcta tcagatttaa catccaatga ggatccggct aaaaatatta tcaaaaaccc
     3121 ttctacggtt aagcagatgg cagaacagat cattgttgca gcagatgcat atttttcttt
     3181 acgtcttgaa gaagatgaat ttcaggagct aatactaaat tatgcaagca ggcatggtaa
     3241 aaaactgttt ggtaagaacg ggaaaatcaa cccgacaatc agaaatatta ttggcaagaa
     3301 acggcgagaa ctggttgaat tgatgcttag aggatatcaa ttatctgtgt tttagattgt
     3361 atgggataaa aaattggcgc ttctgataat ttcaggagcg ttttttttct gaccttcata
     3421 atttgaaaaa atatgttttt aaccatgtgc tttttaaact aatttactgc ctgaataggc
     3481 tcaaataatt tgagaaagtg gtttgtttta atttgaaaat ctacacagta tgatgaaaac
     3541 tttgaaatac tgctggattc cctttggcag tatcagtgga cgaggaaaga aagcatatta
     3601 acaaagagca tgcgagaatt gttgtatgaa gaatctcagg gaataattga tataatctgt
     3661 aaaatcattg tcatggcaca aactattgca atttccactg gaaaagagca ggttgatgaa
     3721 aaattaatac aacaagtagc aagagaacat ctccaattgg ttagaccgat gattttagc
//
LOCUS       DAONVY010000195         3776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16943_length_3776_cov_3.973663, whole genome shotgun sequence.
ACCESSION   DAONVY010000195 DAONVY010000000
VERSION     DAONVY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16943_length_3776_cov_3.973663"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..346)
                     /locus_tag="PLI11_06085"
     CDS             complement(119..346)
                     /locus_tag="PLI11_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPZ52468.1"
                     /translation="MKSATIQLETLACPSCMQKIDKAVKSVDGVDKESVNVMFNASKV
                     KLQFDPEKTTIDNITDSIGKIGYEVKKSVVR"
     gene            complement(368..2221)
                     /locus_tag="PLI11_06090"
     CDS             complement(368..2221)
                     /locus_tag="PLI11_06090"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPZ52469.1"
                     /translation="MQRFLLKHKNKIALIGGVLIALSFLVRWTIGNMFLFEILMIAAS
                     VLGAAPIAIMAFQSLRIKVVSIDVLVTIAVTGAFVIKNYEEAAIVTFLFLFGAYLEQR
                     TLNKTRSAIKELAEMAPESALKLMENGEFEEVDVDEVDIGDVLLVKTGAKVPVDGTVV
                     LGEGSVNEASITGESLPVAKEKGSKVFAGTILDNGTIQIVADRVGEDTTFGRIIELVE
                     EAQDSKSQAERFIDRFSKYYTPFVLVLAFVVGLVTRNVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLFKGSEVISDFSRLDTMVFDKTGTLTVGNPSVMEKEYYGENVE
                     EVLSYLASVEKESDHPLAKAVLEHIGEVETFSVENTGVVKGEGIIATVNGRRIAVGNV
                     ALMEREKVHLDDRMRADIDRFEKNGNSLVLTAVDEELKVLMGVRDKIRPDVKKDLDSL
                     KRLKVKNLVMLSGDNQGTVDLVAKELRLTEARGNMLPEDKSEYVKDIIDKGQIVAFVG
                     DGVNDSPSLALAHVGIAMGSGTDVAIETSDVVLMNSDFGRLPHALGLSKAITRNMRQN
                     IFIAIGVVIVLLVSVLFSDWMNMSIGMFVHEASILAVILNGMRLLRYRLRR"
     gene            complement(2252..2530)
                     /locus_tag="PLI11_06095"
     CDS             complement(2252..2530)
                     /locus_tag="PLI11_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HPZ52470.1"
                     /translation="MARTFDEVKKEHMKTLEMYVPIVARVHGEHHPEFHEVKKVFDEM
                     KGKVDADLKDKFARLRDITGNYSVPEDVCESYEAVYKMLEELDIAYHA"
     gene            2681..3541
                     /locus_tag="PLI11_06100"
     CDS             2681..3541
                     /locus_tag="PLI11_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPZ52471.1"
                     /translation="MENKKKSQRTLILVMIINFLISIIKICLGLYTKSTSMTADGFHS
                     ISDTSSNIVGIIGLHYASKPKDKKHPYGHYKFETLASLFISFMLFALSGQIVVDAISK
                     FINPTITRIPPISIAVLIFTIVVNTVVSVWENKKGKELNSQVLASDSIHTRSDIFVSL
                     GVLVTLILLKLGANPIIDPIASLVVAVFIIHAALKIFKDSSFILVDGTQISEDDIRNI
                     VMEFDEVVDVHKIRSRGSLDNLNIDLHIVVNADLDVEKSHILVHDIEDAMKEKFNKNT
                     QVLAHVEPDN"
     gene            complement(3542..>3776)
                     /locus_tag="PLI11_06105"
     CDS             complement(3542..>3776)
                     /locus_tag="PLI11_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52472.1"
                     /translation="DNPLGGFEVKGLLIGDENHVGDTAHVPSCSEVFEFKGKWYISVA
                     ERLPGNEQYIEFMRFYWNEDGSVSVGDFVREPV"
BASE COUNT         1123 a          927 c          600 g         1126 t
ORIGIN      
        1 caaagttaat gatagctatg tatgattgat gtgatggaaa aaacggcata gccgaatatc
       61 aacgactatg ccgcattttt ataaacaatc ccaatctgac ctatagaggg aattttttct
      121 atcttaccac ggatttctta acctcatacc ctatcttgcc aattgaatcg gtaatgttat
      181 ctattgtggt cttttccgga tcaaactgaa gtttaacctt gcttgcattg aacattacat
      241 tcacactttc cttgtcaaca ccatccacac tctttaccgc cttgtcaatc ttctgcatgc
      301 aggatgggca tgccagagtt tccagttgta ttgtagctga tttcatttag catctctcct
      361 tcatttttta tcttctcaat ctatatctca aaagcctcat gccgttcaat atgacggcca
      421 gaatgctggc ttcatgtaca aacatgccaa tcgacatatt catccagtca ctgaacaaaa
      481 cactcacaag caaaacaatt acaacgccaa ttgcaatgaa tatgttctgt ctcatattcc
      541 tggttattgc ctttgacagc ccaagagcat gtggcagtcg tccgaaatca gagttcatta
      601 gtacgacatc cgaagtttca attgccacat ctgttccgct acccatggca atacctacat
      661 gggcaagcgc aagggacgga ctgtcattaa caccatcacc tacaaaggca acgatttgtc
      721 ctttgtcaat aatatccttg acatattctg acttgtcttc aggaagcatg tttccacgag
      781 cttctgtaag tctcagctcc tttgccacca gatccaccgt accttggttg tcaccagaaa
      841 gcatgacaag atttttcacc ttcagcctct tgaggctatc caaatccttc tttacatctg
      901 gacgaatctt gtcacggaca cccatcaata ctttaagctc ttcatcaaca gctgtcaata
      961 ccagagaatt accgtttttc tcaaacctat caatgtcagc tctcatcctg tcatcaaggt
     1021 gaaccttctc tcgttccata agagccacat tgccaacagc tatcctgcgt ccatttactg
     1081 tggcaattat gccctctccc ttgacaactc ccgtattctc aacagaaaag gtttccacct
     1141 ctccaatgtg ctcaagaaca gcctttgcca gaggatgatc agattccttt tccacactgg
     1201 caagataaga aagaacctct tccacattct ctccatagta ttccttttcc atgacagaag
     1261 gatttcctac tgtcagggtt ccggttttgt caaacaccat ggtatcaagc ctgctgaaat
     1321 cacttataac ttcgctacct ttaaacaaaa ctccatgtct tgcaccattt ccaattcccg
     1381 ccacattcga tacaggaaca ccaattacca aggcaccagg gcatcccagc accagtatgg
     1441 ttattgcaag ttctacgttc cttgtaacaa ggcctaccac aaaagccagc accagtacaa
     1501 aaggtgtata gtatttagag aatcggtcaa tgaatctttc tgcctgggat ttggaatcct
     1561 gggcttcttc cacaagctct attatccttc caaaagtggt gtcttcccca accctgtcag
     1621 caactatctg aattgttcca ttatcaagta ttgtacctgc aaatactttg gaacctttct
     1681 cctttgcaac gggcagagat tcgcctgtga tacttgcctc gttcacactt ccttcaccca
     1741 atacaactgt tccgtctaca ggcacttttg cacccgtctt tacaagaagc acatcgccaa
     1801 tatctacctc gtcaacatca acttcctcaa actctccgtt ctccataagc ttaagagcgc
     1861 tctcgggagc catttcagca agttccttta ttgctgaacg tgttttgttg agagttctct
     1921 gttccagata tgcaccaaac aggaataaaa atgttactat cgcagcttcc tcatagttct
     1981 ttataacaaa cgcaccggtt actgcaatgg taaccaaaac atctatacta acaaccttta
     2041 tccttaaaga ttggaaagcc attatggcaa taggtgcagc acccagtacg gaagcagcta
     2101 tcatcaatat ttcaaacaaa aacatgtttc ctatcgtcca tcttacaagg aaggacaaag
     2161 ctattaacac tccgccaatc aacgctattt tattcttgtg ctttaataaa aaccgctgca
     2221 ttacctatca cccctttaat ttaaattaac attatgcatg atatgcaata tccagttctt
     2281 caagcatttt atatacagct tcatagcttt cacaaacatc ttccggaact gaataattgc
     2341 cggttatgtc gcgcagtctt gcaaacttat ccttaaggtc tgcgtctact ttacccttca
     2401 tttcatcaaa tacttttttc acctcatgaa actcaggatg atgctctcca tgcactcttg
     2461 ccactatggg aacatacatc tccaaagttt tcatgtgctc cttcttaacc tcatcaaatg
     2521 ttcttgccat aatagatacc ctctttctat caattgttat caatcacaag catattatac
     2581 caaccatttt cacagattgc tttgacttga atcacactaa atattttgca ttcataatat
     2641 tacatatgtg tatttaaaat atgcagcatg gagaaatgtc atggaaaaca aaaagaagtc
     2701 acaacgcaca ttaatactag tcatgataat taatttcctc atatccataa taaaaatctg
     2761 tttgggactt tatacaaaaa gtacaagcat gacagctgat ggtttccatt ccatttccga
     2821 cacttcttca aatattgtgg ggattattgg actgcattat gcatcaaagc caaaagacaa
     2881 aaagcaccct tacggccatt acaagtttga aacccttgct tccttgttta tatccttcat
     2941 gctctttgcc ttgagcggac aaatagttgt tgacgcaata tcaaaattca taaatccgac
     3001 aatcaccaga attccaccca taagtattgc cgtattaata ttcactattg tagttaacac
     3061 tgtagtatcc gtctgggaga acaaaaaggg aaaggaacta aacagccagg ttcttgcatc
     3121 tgactccatc catacaagaa gcgacatatt tgtgtcttta ggtgtgctgg ttactctgat
     3181 attattaaaa cttggcgcaa acccaataat cgatccaatt gcatcccttg tggtggctgt
     3241 attcataatc cacgctgcat tgaagatatt caaagacagc agttttattc tggttgatgg
     3301 aacccagata agtgaagatg acatacgaaa catagtcatg gagtttgatg aagttgtgga
     3361 tgtacataaa atacgcagca gagggagttt ggacaacttg aacattgatt tgcacatagt
     3421 agttaatgca gaccttgatg tggaaaagtc gcacattctc gtgcatgata ttgaggatgc
     3481 tatgaaagaa aaattcaata aaaacacaca ggtgttggct catgtggagc ctgataatta
     3541 attaaacagg ctcccttaca aaatcgccga cacttacaga accatcctcg ttccaataaa
     3601 atctcataaa ctcaatatat tgctcatttc caggcaatct ttctgcaaca gaaatgtacc
     3661 acttaccctt gaattcaaac acctcactgc aggaaggcac atgagcggta tcccctacat
     3721 ggttctcatc gcctataaga agacctttga cttcaaaacc accaagcgga ttgtct
//
LOCUS       DAONVY010000196         3775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_16949_length_3775_cov_4.340323, whole genome shotgun sequence.
ACCESSION   DAONVY010000196 DAONVY010000000
VERSION     DAONVY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16949_length_3775_cov_4.340323"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLI11_06110"
     CDS             complement(<1..504)
                     /locus_tag="PLI11_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HPZ52473.1"
                     /translation="MAIKTEYSVEEIFVDHISNKTIDVLVSDINPMHAALPFVVTSMG
                     LYTEGPNFRGWNEGYLNYSLHFTLSGSGLVKYNGKEYVIKPGAVFFKNNRERKYFCTY
                     GDHWKFCYINCIGTSTMIFDKLLNSNDKLNIIYTNNINELFNSFVELNVLIKKNDILS
                     YIESSTII"
     gene            complement(584..>1783)
                     /locus_tag="PLI11_06115"
     CDS             complement(584..>1783)
                     /locus_tag="PLI11_06115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPZ52474.1"
                     /translation="ILFVVIILTCDYTPVDTSSLPQEYLYNDRINKKEVEKLKEILEN
                     LEDYEKDCEEYDLHKYMKYLWDTNIVYNESVYPLENEDGTISPIPLMYEPVCILSVRD
                     SSLETEYKKGVDYDLVNGKLVIKETGSIPRVKHKEYWLDHEISGSSFPRTGGGYILYG
                     QGEPFLSKQLAVTYIHLGEWKGEIPKGKSSLLPKTTAKLENKEELKIVFYGDSIGEGC
                     NSSGFHKVEPFADDWCSMATKYLKEKYDNDYITMTNASVGGMDSAWGYNNYKNVSRLK
                     PDLVVVAFGMNDGSLRVPVNSYTWNIGKIVERIRSENEECEFVLVATMLANEDVAGFA
                     GFQEDYLPALLGLEESGVAVADMTTVHKYLLSKKRYYDMTGNNVNHPNDFLARFYAQV
                     VIETITG"
     assembly_gap    1784..1883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1916..2779
                     /locus_tag="PLI11_06120"
     CDS             1916..2779
                     /locus_tag="PLI11_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52475.1"
                     /translation="MKKILITIVFILILIIPAYASEAPGYVVDLSTKGGYEAYVELVD
                     LDKYPVGITNGSEFTTHGMYIEYKDANGKGLGEYRWTVDYTFNEEDKCITFVGKPEEL
                     KTSQKDDLRLTIQTEFSPKELPYVVICYRITDKAPISTNHIYLRDNRKNKEYSGVPTT
                     WTPHQLVANGQWNYRILDMQKELAAITGQCMGIRLPICTREGDKFDVKFVAAFATKEA
                     AENFDIEAFETYLASITTPEPTEDNTKTPGPYEGGKSSLGVWGTVLFVAVAIIVVVGV
                     TALYRFLAKKL"
     gene            complement(2783..>3775)
                     /locus_tag="PLI11_06125"
     CDS             complement(2783..>3775)
                     /locus_tag="PLI11_06125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPZ52476.1"
                     /translation="YGEIGSNVYVSTDKGKTWSFRGGVKGIQGSSSADENMVVELGDG
                     SLMMYIRTTIGIEKSYSYDKGFTWSDAVNANITKTVSRFFLSKLDSGKLLLVYHNPPA
                     KDGSRSYLTASISEDDGKTWKGNLIIDERNGVSYPDGFQTKDGKIYVIYDRNRGSDME
                     ILMAVITEEDILAGRIVNPESRLKVLVNNNGKQPPKTPEDLKNTGITPADGGQVAVLE
                     NNKTIFSNRSESRVFVNLPKKFHGMYYVYSELEYSSARIDKDGDVYIFTKRNNGVVAL
                     HGHLTSLGFVQDKSVEPFRMFDQATGEDTVVYHKRCKAGEEISFGNWVVIAYKP"
BASE COUNT         1095 a          817 c          653 g         1110 t
ORIGIN      
        1 tatgatagtg cttgattcaa tataagacag gatgtcgttc tttttaatta aaacattaag
       61 ttcaacaaaa ctgttgaaaa gttcgtttat attgtttgtg tatataatat tcagcttgtc
      121 attggaatta agtaatttat caaaaatcat tgtgctcgtt ccaatgcagt taatatagca
      181 gaacttccag tggtcaccat aagtgcaaaa gtactttctt tctctgttgt tcttaaagaa
      241 tacagctccc ggctttatta cgtactcctt gccattatac ttgaccaatc cggaaccaga
      301 aagggtaaaa tgaagcgaat aattcaaata cccttcattc catccacgaa agttgggtcc
      361 ttcagtatac aaccccatgg aagtaacaac aaaaggaaga gctgcatgca tgggatttat
      421 gtcgctaact aaaacatcga tggttttatt tgaaatatga tcgacaaata tttcctcaac
      481 agagtattca gtttttattg ccataagttc acatccttaa atcgttaacc caattatatt
      541 ttaacaaaat tatcctgtca acaacacatc actcttccct ccctcatcca gttatggttt
      601 caatgactac ctgcgcataa aatctggcca ggaaatcatt tggatgatta acattgttgc
      661 cggtcatgtc gtagtagcgt ttttttgaaa gcagatattt gtgtaccgtg gtcatatccg
      721 ccactgcaac acctgactct tccaatccca aaagagctgg cagatagtct tcctgaaaac
      781 ctgcaaatcc agccacatcc tcatttgcaa gcattgtggc aacaagaaca aattcacatt
      841 cctcattttc agagcgtatc ctctccacaa ttttacctat gttccatgta tatgaattca
      901 caggaactct caaacttccg tcattcatgc caaaagccac taccaccaga tccggcttta
      961 accttgacac atttttataa ttattatacc cccaggcaga atccatgccg ccaacagaag
     1021 cgtttgtcat tgtaatgtaa tcattatcgt acttttcttt cagatacttg gttgccatgc
     1081 tgcaccagtc gtctgcaaaa ggttctactt tgtgaaatcc actgctgttg catccttctc
     1141 ctatactgtc gccatagaat actatcttca gttcttcctt gttttcgagt tttgcagttg
     1201 tctttggcaa cagactgctt ttgcctttgg gaatttcacc tttccactct cccaggtgaa
     1261 tatatgttac tgccagctgt tttgataaaa agggctcgcc ttgtccgtac aatatgtatc
     1321 caccaccagt acgcggaaaa ctgcttccgc ttatctcatg atccagccag tactctttgt
     1381 gtttgaccct ggggattgaa cctgtctcct tgatgactaa ttttccgttt actaaatcat
     1441 aatccacacc ttttttgtat tcggtttcaa gtgatgaatc tcttaccgat agaatacata
     1501 caggctcata cataagcgga ataggcgaaa ttgtgccgtc ttcattctcc agaggataga
     1561 cagattcatt gtaaacaata ttggtatccc acagatactt catgtatttg tgaagatcat
     1621 actcttcaca atccttttca taatcctcta gattctcaag aatctctttc aacttttcca
     1681 cttccttttt gttaatcctg tcgttataca aatattcctg cggcaaagat gaagtatcca
     1741 ccggagtata atcgcacgta agaataataa cgacaaagag aatnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnntgaaaac aagctcgttt ggaggagata ggctgatgaa
     1921 aaaaatattg ataacaattg tgtttatcct gattctaatt atacctgcat atgcaagtga
     1981 agctcctggt tatgttgtgg atctcagtac aaagggagga tacgaggcat acgtagagct
     2041 ggttgacttg gacaaatatc cagttggtat taccaacggt tctgaattta caacccacgg
     2101 catgtatatt gaatacaagg atgcaaatgg caagggtctt ggcgaataca gatggactgt
     2161 agactatacc ttcaatgaag aagacaaatg cataacattt gtaggaaaac cggaagagct
     2221 gaaaacaagt caaaaagacg acctaagact aacaattcaa acagagttca gtccaaaaga
     2281 actgccttac gttgttatct gctacagaat aacagacaaa gctcccatat ctacaaacca
     2341 tatctatctt agagacaaca gaaaaaacaa agagtacagc ggagtgccaa ctacatggac
     2401 accccaccaa cttgtagcaa acggacagtg gaactacaga atacttgaca tgcaaaaaga
     2461 gctggctgcc ataactggcc agtgcatggg aataaggctt cccatatgca ccagggaagg
     2521 cgacaagttt gatgtaaagt ttgttgcagc ttttgcaaca aaagaagctg ctgaaaactt
     2581 tgatatagaa gcattcgaaa catatctggc ttcaataacc acacccgagc caactgaaga
     2641 taataccaaa acaccaggac cttacgaagg tggaaaaagc tctttaggag tatggggtac
     2701 agttcttttt gttgctgttg caattattgt cgtagttggt gtaacagcac tctacagatt
     2761 cctggcaaag aaattatagt atttatggtt tatatgctat aaccacccag ttcccaaaac
     2821 ttatctcttc accagctttg cagcgcttgt gatataccac ggtgtcctcg cctgttgcct
     2881 ggtcaaacat tctaaacggt tcaacggatt tgtcctgcac aaaaccaaga gaggtcaaat
     2941 gaccatgcag agcaacaact ccgttgtttc tttttgtgaa gatgtataca tctccatcct
     3001 tgtcaattct tgcagaactg tattccagtt cagaataaac atagtacatc ccgtgaaact
     3061 tctttggcag attgacaaat accctggatt ccgaacgatt tgaaaagatg gttttgttgt
     3121 tttccaaaac agcaacctgc ccaccgtcag caggagttat tcctgtattt ttcaaatctt
     3181 caggggtttt tggcggctgt ttaccgttgt tgttaacaag aactttcaat ctcgactctg
     3241 gattaacaat cctgcctgca agaatatcct cctccgttat aactgccata agtatttcca
     3301 tgtcggaacc tctgtttctg tcatagatga catatatctt tccatccttt gtctggaagc
     3361 cgtcaggata agacactccg tttctctcat ctatgattag attgcctttc catgtctttc
     3421 cgtcatcttc cgatatggag gcggttagat aggaacggct gccatccttt gctggagggt
     3481 tgtgatacac caaaagcagt ttgcccgaat caagttttga caaaaagaat ctggatacag
     3541 tctttgtaat atttgcattt acagcatcag accaggtaaa acccttgtca taagaatagc
     3601 tcttttcaat accgatggtg gttctgatat acatcataag gcttccatca ccaagttcca
     3661 ccaccatgtt ttcatctgca ctgcttgaac cttgaatgcc tttaacaccg cctctaaaac
     3721 tccaagtctt gcccttgtct gtggatacat aaacattgga acctatttca ccata
//
LOCUS       DAONVY010000197         3761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17030_length_3761_cov_3.039935, whole genome shotgun sequence.
ACCESSION   DAONVY010000197 DAONVY010000000
VERSION     DAONVY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17030_length_3761_cov_3.039935"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /gene="mutL"
                     /locus_tag="PLI11_06130"
     CDS             <1..1551
                     /gene="mutL"
                     /locus_tag="PLI11_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPZ52477.1"
                     /translation="LVSKTAEAPVGVCVHLEGGDLIEVSEIGCAKGTTLEVMDLFFNT
                     PARYKFLKKDHVEASYVTDAIEKIALANPDVSFKLVSNGKTVLQTPGNGDLQSTIYAI
                     FGRDVATKLKEVKYEESHINVNGYVGDISLMYSNRNRQIFFVNGRYIKNKTLSAALDK
                     AYSTLAMKGKFPFCVLSVKINPMHVDVNVHPAKIEVRFDNEGLVFEVIYRAVTSALFE
                     DRRKSSIATPKTIQNHTQSTERSYDTKPVTFETFEQYLKIKEEEIPLEVEVEVEVEEK
                     EEEIKEEAVAEQIPIMQSAVEVAKETVVAESLPQENIIQERTENKKRENEVFEFARII
                     GQVWNAYILFEHEGKLYMMDQHAAHERIMYEELRRIINGSEPMVQMLLSPITMELSPS
                     EFQTLKNNFDYFARLGMEMEEFGTASVIIRTLPTILLDENIHSFIQEGLDNIISGGET
                     SLYNDETIFQMACKAAVKANKRLDLREMEELIRRMSTLDNPFTCPHGRPIVIEITKYE
                     IEKRFKRK"
     gene            1548..2477
                     /gene="miaA"
                     /locus_tag="PLI11_06135"
     CDS             1548..2477
                     /gene="miaA"
                     /locus_tag="PLI11_06135"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPZ52478.1"
                     /translation="MIKAIVITGPTASGKTGISIDLAEKLNGEIVSCDSMQVYKGMDI
                     GTAKPVIEERRGIPHHMLDIVEPYETFDAATYKDMAVCCIKEIANRGRTPILVGGTGL
                     YVNSLVDNIQYVESESDSELRKALYDYAKKHGAEALHAKLKEIDPKAAENIHANNIKR
                     VVRAIELSKLTGMTQEERNALSKSVKSDIEYVVYAVQMDRELLYKRIDERAEKMVEMG
                     LVEEVTGIREKCIEAYLKGISPSPTLSKTALQGIGYKEILEYLDNKCTLEESIDKIKL
                     NTRRYAKRQITWFRKPKWVNWVTIEELKNIYSL"
     gene            complement(2472..3113)
                     /gene="lexA"
                     /locus_tag="PLI11_06140"
     CDS             complement(2472..3113)
                     /gene="lexA"
                     /locus_tag="PLI11_06140"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPZ52479.1"
                     /translation="MARKKNTPTPKQQEILDFIEAYIRDNGYPPSIREICAAVNLKSS
                     STVYTHLEALKRKGYIERDDSKTRGIRLTGTKNVVNPFLNIDFDAPVVQVPVIGKITA
                     GAPILAVENIERTFPVPRDFTSSKETFMLNVVGDSMVEAGILDGDMVLVARQNTANDG
                     DIVVALLEDEATVKTFYREENCYRLQPENRYLKAIYVNELIILGKVIGVFRKL"
     gene            complement(3196..>3761)
                     /locus_tag="PLI11_06145"
     CDS             complement(3196..>3761)
                     /locus_tag="PLI11_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52480.1"
                     /translation="EQLLYSTENDNDMEKEPAIPNSDQYDENLDPMLDEMETTNWSEQ
                     NTENTEQLEVQEEVQDIHTEEVDEEQAGDNDVLEILEFSVPEIEEKEEKTELEKVYEM
                     FDVSVPKRSIKNDDFDNISFSATNIYPKEDSGYSEKKFTLKRAYINSGLKFSKEDSKV
                     DSEETGNTIDAMVDMLFENKNDKKRKK"
BASE COUNT         1201 a          711 c          825 g         1024 t
ORIGIN      
        1 cttgtaagta aaactgcgga agcccctgtt ggcgtatgtg tgcacctgga aggtggagat
       61 ctgatagagg ttagtgagat aggctgcgca aagggtacca cgttggaggt aatggatctt
      121 ttcttcaaca cacctgcccg atacaaattc ctaaaaaagg accatgtgga ggcatcctac
      181 gtaaccgatg ccattgaaaa gatagcgctg gcaaacccag atgtgtcttt caaacttgtt
      241 tcaaatggaa aaaccgttct acaaactccg ggcaacggag atttgcaaag cacaatatat
      301 gcgatattcg gccgtgacgt cgccactaaa ctgaaagagg taaagtatga ggaatcccat
      361 ataaacgtaa atggttatgt gggggatatc tctcttatgt attcaaacag aaacaggcag
      421 atattttttg taaacggcag atacataaag aacaaaacct tgtccgctgc tttggacaag
      481 gcctacagca ccctagccat gaaggggaaa tttccttttt gcgtattgtc ggtaaagata
      541 aatccaatgc atgtggatgt gaatgtgcat cctgccaaga tagaggtcag atttgacaac
      601 gaaggccttg tttttgaagt tatatacagg gcggtgacga gcgcattgtt tgaagacaga
      661 agaaagtctt caatagctac tccaaaaacc atacaaaacc atacccaaag tacggaaagg
      721 agttatgaca ccaaacctgt tacatttgag acctttgagc agtacctgaa gataaaggaa
      781 gaggaaattc ctctggaggt tgaagttgaa gttgaagtgg aagaaaaaga agaggaaata
      841 aaggaagagg cggtagctga gcaaataccg ataatgcaaa gcgcagttga agtcgctaag
      901 gagacagtag ttgcagagtc cttgcctcag gagaatataa tccaggaaag gacggagaat
      961 aagaaaaggg agaatgaagt atttgaattt gccagaataa ttgggcaggt atggaatgca
     1021 tacattttgt tcgagcatga gggcaagctc tacatgatgg atcagcatgc tgctcatgaa
     1081 agaataatgt atgaagaatt aagaagaata ataaatggaa gcgagccgat ggtacaaatg
     1141 cttttgtctc caataacaat ggagttgtca ccgtcagagt ttcagacact aaaaaacaac
     1201 tttgactatt ttgccagact gggaatggaa atggaggagt ttggcacggc ttcagttatc
     1261 attagaacac tgccaactat tctgcttgac gaaaatatac actcgttcat ccaggaaggt
     1321 ctcgacaata ttatttcagg tggagaaacc tctttgtaca atgacgagac aatattccaa
     1381 atggcttgta aggcagcagt aaaagcgaat aaaaggttgg acttaaggga gatggaggag
     1441 ctgattagaa gaatgagcac attggataat cctttcacct gtccgcatgg cagaccaatt
     1501 gttattgaga ttacgaaata tgagatagag aaaaggttca agagaaaatg ataaaggcga
     1561 ttgtgattac aggaccgacc gcatccggaa agacgggcat atcaatagac ttggcggaga
     1621 aactgaatgg ggagattgtt tcctgcgact caatgcaggt gtacaaaggc atggatatag
     1681 ggacagcaaa acctgtcatt gaggaaaggc gtggaatacc ccatcatatg ctggatattg
     1741 tagagccata tgaaaccttt gacgctgcca catacaagga catggctgtt tgctgcataa
     1801 aagaaattgc aaatagaggc aggactccta tactggttgg gggtactgga ttatatgtta
     1861 attcccttgt tgataacata caatatgtag aatccgaatc agattctgaa ctaagaaaag
     1921 ctttgtacga ttatgcaaaa aaacacggag ccgaagcact tcatgcaaaa ttgaaggaaa
     1981 ttgacccaaa ggctgcggaa aacatacatg caaacaatat aaaaagggtt gtaagggcaa
     2041 tcgagctgtc aaagctgaca ggtatgactc aggaagaaag aaacgcattg tcaaaaagcg
     2101 tcaaaagcga tatagagtat gtagtatacg cagtacaaat ggacagggag ctattgtaca
     2161 aaagaataga cgaaagggcg gagaaaatgg tagagatggg cctggttgaa gaagtaacag
     2221 gcataaggga gaaatgcatc gaggcctatt taaaaggcat aagcccctcg cccacactga
     2281 gcaagacagc attgcaggga attggataca aggagatcct tgagtatctt gataacaagt
     2341 gtactctcga agagagtatt gataaaatta aattaaatac aaggagatat gcaaaaagac
     2401 aaataacctg gttcagaaaa cccaaatggg ttaactgggt aacaatagag gagttgaaga
     2461 atatatattc attatagctt tctgaacact ccaataacct ttccgagaat aatcagctca
     2521 tttacgtaaa tcgccttcag atacctgttt tcaggctgga ggcgataaca gttttcttcc
     2581 ctgtaaaagg tttttactgt ggcctcgtcc tcaagaagag ctacaacaat atcgccatca
     2641 tttgcagtgt tctgtcttgc tacaagaacc atgtctccat caaggatgcc tgcttcgacc
     2701 atgctgtcgc ccacaacatt aagcatgaat gtttccttgc tgctggtaaa atcccggggg
     2761 acaggaaatg ttctctcaat attttcgaca gcaagaattg gcgcgccagc agtaatcttc
     2821 cctataactg gaacctgaac tacaggtgcg tcaaaatcaa tattcaaaaa aggattcaca
     2881 acatttttgg taccagtaag ccttatacct cttgtttttg aatcatctct ttctatgtaa
     2941 ccttttctct tcaatgcctc aagatgtgta tatacagtgg aggatgattt caagttgaca
     3001 gcagcacaga tttctctaat tgaaggcgga taaccgttat ctcgtatata tgcttcaata
     3061 aaatccagaa tttcctgctg tttcggtgtc ggtgtgttct tctttctagc catttaagct
     3121 gaaacctcct attatttgtt atgaacagta tagcatacta aaatgttccg agcaaacttt
     3181 ttatcgaaca aatatctact ttttacgttt tttatcgttt ttattttcga acaacatgtc
     3241 aaccattgca tctatggtgt tcccggtttc ctcagaatca acttttgagt cttcctttga
     3301 aaacttcaaa ccactgttaa tatatgccct cttcaaggta aacttctttt cactgtagcc
     3361 cgaatcttct ttaggatata tgttggttgc agagaaggat atgttatcaa aatcgtcatt
     3421 ctttatacta cgtttcggta cacttacgtc aaacatctca tatactttct caagctcagt
     3481 cttctcttcc ttctcctcta tctcaggtac tgaaaactcc agaatttcaa gtacatcgtt
     3541 gtcaccagct tgctcctcat caacttcttc agtatgtata tcctgaactt cttcttgaac
     3601 ctcgagctgc tctgtgtttt ctgtgttttg ctcggaccaa tttgttgttt ccatttcatc
     3661 cagcataggg tcaagatttt catcatattg gtccgagtta gggatggctg gttctttctc
     3721 catatcgttg tcattttctg tactgtacaa tagctgctct g
//
LOCUS       DAONVY010000198         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17045_length_3759_cov_2.681425, whole genome shotgun sequence.
ACCESSION   DAONVY010000198 DAONVY010000000
VERSION     DAONVY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17045_length_3759_cov_2.681425"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLI11_06150"
     CDS             complement(<1..610)
                     /locus_tag="PLI11_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPZ52481.1"
                     /translation="MLVRLIKYFKKYKFYAILCPILMLVEVLCDVLMPYMMALIVNRV
                     SDSVRANTSFTNDDTRYIITVGAVMILLALIALLCGALAARLAAAAAQGGGTELRYDL
                     FKQIQTFSFADLDKFSVPSLITRLTTDITNIQMAGMMSLRMLIRAPFMLLFALILALS
                     INSELALVFLVAIPILGIALYFIIRTAHPRFRKLQKKFDALNA"
     gene            770..1669
                     /locus_tag="PLI11_06155"
     CDS             770..1669
                     /locus_tag="PLI11_06155"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPZ52482.1"
                     /translation="MLNFKEVELSDINRLGEYLKYQEFRTCDFTAGAIYMWRDYFYSE
                     FAIHKDMLIFKVAYPTRGTSFSYPIGKGSTDEAMKAIEEYTRANGLELKFCTLPEKAM
                     EYVKFRYGDKCHFITSRDWYDYLYNAESLKTFKGKKYHGQRNHVNRFKREYPNHKYHR
                     ITQENLGRVVEAFDKYFVPSDDASSSELKEARRAREYLNYFLSFNHLGGFIEVDGEVI
                     AFALGEVVKDTLFVHVERAVTDYHGSYQMIVSEFAKDYATDGVIYINREEDVGDPGLR
                     KSKLSYKPIRLLEKYCAHIELFE"
     gene            1705..2364
                     /locus_tag="PLI11_06160"
     CDS             1705..2364
                     /locus_tag="PLI11_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HPZ52483.1"
                     /translation="MNLTSKLFSLDKSIAGDLLKNYEFPWECLKDIGKYILEAALLLP
                     YDFVQIKENVWVANSAKISLTACIEGPAIICRGAEIRHCAYIRGNVIVGEDAVVGNST
                     EVKNSILFNDAQVPHFNYVGDSILGYKAHFGAGVITSNVKSDKKNISVRMEDGSIIPT
                     NLRKFGALVGDFVEVGCNTVLNPGTIIGRNTNIYPLSCVRGYVKENSIYKRLGEVVDK
                     L"
     gene            2379..3275
                     /locus_tag="PLI11_06165"
     CDS             2379..3275
                     /locus_tag="PLI11_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPZ52484.1"
                     /translation="MSKETQKSKFDRPRKFGDRKDGWRVRNIDPYMNIVPVLMPGRNV
                     SCIYFSETIDATNLVDFVKRMNEDEQAKREKGIRKYTYFGVFMAALVRTLAMRPHLNR
                     FISSGKIYQRRNIKLCFVAKKDMTEDAPETDVLAVFKRTANLDDVMRKLQGGIREAKE
                     GDDDTTDVLNALFKLPTFMLRGFKGLMDLLLKFDLFPKSLEKVDPMQSSAFVSNLGSI
                     NLVNVPFHHLYERGTCSLFVVMGKIYPEGDRYKMNFTVTLDERVSNGFYYIRSIEFFK
                     ELLSNPEVLLESPSEEEIPLDI"
     gene            3297..>3759
                     /locus_tag="PLI11_06170"
     CDS             3297..>3759
                     /locus_tag="PLI11_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52485.1"
                     /translation="MQSKVGRFFIGIMLAAAAFLVYFLVSNGISIVILFSISPVILSQ
                     NPEMSQQELQIKLMEVFEALVPTIFVLTTIILVIIFYAFFIFINKNLNEYTRLKKIQW
                     PFVMLSFFLGYALNMASIAVMELVPIPETWIEDHDQHTSFYGPVLFIILAVV"
BASE COUNT         1158 a          611 c          886 g         1104 t
ORIGIN      
        1 aggcgttgag ggcgtcaaac ttcttctgaa gttttctaaa cctgggatgg gcagttctta
       61 ttatgaaata caaagcgatg cccagtattg gaattgcgac aaggaataca agggcaagct
      121 cgctgtttat gcttagtgcg agtatcaagg caaaaagcag catgaagggt gctcgtataa
      181 gcatcctcaa agacatcatt cctgccattt gaatattggt tatgtctgta gtgagtctgg
      241 taataagtga tggaacactg aatttgtcca gatctgcaaa agaaaaggtc tgtatctgtt
      301 tgaaaagatc atatcggagt tcggtgcctc caccctgggc tgccgccgct gccagacgag
      361 cagcaagagc tccgcacagc agtgcaatca atgccaggag tatcatcaca gcacctacgg
      421 ttataatata tcttgtgtca tcatttgtga aacttgtgtt cgcccttaca gaatcgctga
      481 cccggtttac tatcaaggcc atcatgtagg gcatgagcac atcgcacaat acttccacca
      541 gcattagtat agggcataaa atagcataaa atttgtactt tttaaagtat ttaatcagtc
      601 gaacaagcat ccgatatatt tcctccactt cataataatt atcatagaat caaatatatc
      661 tttttttaaa tatttaatca acagtttggt gttaaaaatg tatgaatgtg aaaaaaatgc
      721 cgatatggta taatagggcg aatgtattat gattaatcaa ggaggactta tgcttaattt
      781 caaggaagtg gaactttctg atataaatag acttggtgag tatctgaaat atcaggagtt
      841 tagaacctgt gattttacag caggcgcaat atacatgtgg agagactatt tctattcaga
      901 atttgctata cacaaggata tgcttatatt caaggtggca tatcctacaa gagggacaag
      961 tttcagctat cccataggca aaggctccac cgatgaggcg atgaaggcaa tagaggagta
     1021 cacaagagca aatggcttgg agctgaagtt ctgtaccctg cccgaaaagg caatggagta
     1081 tgttaagttt cgatatggtg acaaatgcca ctttataacc agcagggact ggtatgatta
     1141 tctgtataat gcagaaagct tgaaaacatt caaagggaag aagtaccacg gtcagaggaa
     1201 ccatgtaaac aggttcaagc gtgaatatcc aaaccacaaa tatcatagga ttacacaaga
     1261 gaatctgggc agggtcgtcg aggcttttga taagtatttc gtaccatccg atgacgcttc
     1321 cagttcggaa ctgaaggagg caagaagggc aagggagtat ttgaactact ttttgagttt
     1381 caaccatctt ggtggattta ttgaagtgga tggtgaagtg attgcttttg cactgggaga
     1441 ggtggttaag gatactcttt ttgtacatgt ggagagggct gttaccgatt accacggctc
     1501 ctatcagatg attgtaagtg agtttgccaa agactatgca accgacggcg tgatatatat
     1561 taacagggag gaagatgtgg gagatcccgg tcttagaaaa tcaaagctgt cttacaaacc
     1621 aataagactt cttgaaaaat attgcgctca tattgaactt tttgaataaa gaatatataa
     1681 tgagaaaaaa caaggaagtg tcttatgaat ctaacctcaa aacttttttc tctagacaag
     1741 tccatagcag gtgatttgct taaaaactat gagtttccat gggaatgcct taaggatatt
     1801 ggcaaatata ttcttgaggc agctctttta cttccatatg actttgtgca gataaaagaa
     1861 aatgtatggg ttgcaaatag tgcgaaaatt agtcttacag cctgtataga aggtcctgca
     1921 atcatatgca ggggagcaga gataaggcat tgtgcttaca ttagaggcaa tgttattgta
     1981 ggtgaagatg ctgttgtagg gaattcgact gaggtaaaga attccattct gtttaatgat
     2041 gcgcaggtac cccatttcaa ttatgtagga gattccattc taggctacaa agcccatttc
     2101 ggtgcgggag tcattacttc gaatgtaaag tcggacaaga agaacatatc tgtgaggatg
     2161 gaagatggca gcataatccc aaccaatttg agaaagtttg gtgcattggt tggagatttt
     2221 gtggaagtag ggtgcaatac ggttcttaat cctggaacaa ttatcggacg taatacgaat
     2281 atttatccct tgtcgtgcgt aagaggatat gtgaaggaaa acagcatata caagaggttg
     2341 ggagaagtag tggacaaatt atagaaggat ggtgtgaaat gtcaaaagag acgcagaaaa
     2401 gcaagttcga cagaccaaga aagtttggtg acagaaagga tggctggagg gttaggaata
     2461 tagatccata tatgaacata gttcctgttt tgatgcctgg cagaaatgtt tcctgcatat
     2521 atttcagtga aactattgat gctacaaacc tggttgattt tgtgaaaagg atgaacgagg
     2581 atgagcaggc aaaacgggag aagggaataa gaaagtatac atatttcgga gtgttcatgg
     2641 ctgcgctggt taggaccctg gcaatgcgtc ctcatcttaa tcgtttcatt tcttcaggca
     2701 aaatatatca gaggaggaat ataaagcttt gttttgtggc gaaaaaggat atgacggaag
     2761 atgcgcctga aaccgatgtg cttgcggttt tcaaaaggac tgcgaatctt gatgatgtta
     2821 tgaggaaact gcagggcggt atcagggaag caaaagaggg cgatgatgat accacggatg
     2881 tattgaatgc tcttttcaag ctgccaactt tcatgctcag gggattcaag gggctcatgg
     2941 atttattgtt gaagtttgac ttgtttccaa aatctttgga gaaggtggat ccaatgcagt
     3001 caagcgcttt tgtaagcaac ctgggcagca taaaccttgt caatgttcct ttccatcatt
     3061 tgtatgaaag aggtacttgt tccttatttg tagtaatggg caagatttat cctgaaggcg
     3121 atagatacaa gatgaatttt acggtaacgc ttgatgaaag agtgtcgaac ggcttttatt
     3181 acatacgttc cattgaattt tttaaagagc ttctgagtaa tccggaagta ttgctggaaa
     3241 gtccttcgga agaagaaatt ccattggata tttaatatat ttttgtgagg tgtggtatgc
     3301 aatctaaagt tggtcgtttt tttataggta ttatgttggc agctgcggct ttcctggtat
     3361 actttctggt aagcaacggt attagtatag ttattttgtt ctcaataagt ccggtgattt
     3421 tatcacagaa tccggagatg agtcagcaag agttgcagat taaactgatg gaagtttttg
     3481 aagctctcgt tccaaccata tttgttttaa ccacaataat tttagttata attttttatg
     3541 ctttttttat atttatcaac aagaatctta acgagtatac aaggctgaaa aaaatacagt
     3601 ggcctttcgt aatgttatcc tttttccttg gatatgcatt gaacatggca tccatagcag
     3661 tgatggaact tgtgccgata cccgaaacct ggatcgagga ccatgatcaa catacatctt
     3721 tttatggccc ggtattattt atcatactgg ctgttgttg
//
LOCUS       DAONVY010000199         3754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17082_length_3754_cov_2.682887, whole genome shotgun sequence.
ACCESSION   DAONVY010000199 DAONVY010000000
VERSION     DAONVY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17082_length_3754_cov_2.682887"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PLI11_06175"
     CDS             complement(<1..1202)
                     /locus_tag="PLI11_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPZ52486.1"
                     /translation="MDQNRAPLFEAVKSHKVERVVAFDVPGHKGGRGNPELAEFLGEN
                     CLRVDYNSRKPLDNLIHPSSVIKEAQKLCAEAFSAREAFFMVNGTSSAVQTMIMSTCK
                     AGDKIIIPRNVHRSAINALVVIGAKPIYINPGISTKLGIPLGMSVADVKKALDEHPDA
                     KAVFVNNPTYYGICSNLKEIVRLAKDKGVKVLVDEAHGTHFYFGDDMPVSAMAVGADM
                     AAVSMHKTGGSLTQSSLLLCGPDVDADYVRQVLNLTQTTSGSYLLMMSLDVARRNLAL
                     RGREMIAKTVEYADYARKEIDQIGGYYTFSSDLVNGDTVYAYDNTKLTVHTRNIGLAG
                     IEVLNILRDEYGIQLEFGDMGNILAIISAGDRYQDIERLISALSEIKRVYAKESEEVF
                     DYDYMVPDV"
     gene            complement(1222..2043)
                     /gene="speD"
                     /locus_tag="PLI11_06180"
     CDS             complement(1222..2043)
                     /gene="speD"
                     /locus_tag="PLI11_06180"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPZ52487.1"
                     /translation="MMLELAKKLALYGFNNLTKTLSFNIYDVSYAKTDREQRDYIAYI
                     DEQYNSERLTSILCKVTELIGAHILNISKQDYEPQGASVNILIAEGALSESQVDETCN
                     LGKGISGYKETIHAHLDKSHVTVHTYPEAHPDNAISTFRVDIDVSTCGRVSPLKALNY
                     LIESFDSDIITIDYRVRGFTRDLEGKKYYIDHDINSIQDYIDEETLNMYDAIDVNVYQ
                     ANIFHTKMLIKEIDLQNYLFNQDVYELPPKQRLEITDRLRKEMIEIFSGMNIYQK"
     gene            2233..2544
                     /gene="trxA"
                     /locus_tag="PLI11_06185"
     CDS             2233..2544
                     /gene="trxA"
                     /locus_tag="PLI11_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPZ52488.1"
                     /translation="MSVITLTKDNFEDEVLNSDVPVLVDFWASWCGPCRMISPIIEEI
                     AAEENGIKVGKVNVDDEPELASTYNIMSIPTIMVFKNGEVVNVAVGARPKSALLSLVK
                     S"
     gene            2549..3055
                     /locus_tag="PLI11_06190"
     CDS             2549..3055
                     /locus_tag="PLI11_06190"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HPZ52489.1"
                     /translation="MENSKFGFVRHPYEPVFNIDSKVLILGTVPSVASRKYGFYYTHP
                     QNRFWKVLSSILNEEFPDTIEKKTDMLLKNHIALWDVLKETQIKGSSDSSIKNPKPND
                     INRILNSTDIKAIFTTGKKAFELYNKLCYPKTKFKAICLPSTSPANCVYTLDRLIDEY
                     SIILKYIQ"
     gene            3065..3628
                     /locus_tag="PLI11_06195"
     CDS             3065..3628
                     /locus_tag="PLI11_06195"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPZ52490.1"
                     /translation="MKDIILASKSPRRIELFKKLNLPFSCITPDIDEAMDLSCGLENA
                     VAKLAFRKANSIIEKHHNTIVIGADTIVTLENFILGKPRDKQDAFNMLMQLSGKTHKV
                     MTGVCILTDNRMESFCDTSEVTFYHLTDNEIKKYIATGEPMDKAGAYGIQGQGALLVK
                     HINGDFYSIMGLPIARINQILKSYTID"
BASE COUNT         1123 a          812 c          666 g         1153 t
ORIGIN      
        1 acatcgggaa ccatatagtc gtagtcaaac acctcttcag attcttttgc atataccctt
       61 tttatctcag acagtgccga tatgagcctt tctatgtcct gatacctgtc ccctgcagat
      121 ataattgcaa gaatgttacc catgtcgcca aattccaact gtattccgta ttcatctctc
      181 aatatgttga gtacttcaat acctgcaagc cctatgtttc tagtgtgtac tgtcagcttt
      241 gtgttgtcat aggcgtatac ggtatcacca tttacaagat cgcttgaaaa ggtataatag
      301 cctccaatct gatctatttc cttccttgca taatctgcat attccacagt tttggctatc
      361 atttccctgc ctcttaaggc aagatttctt ctggctacat caagagacat cataagaaga
      421 taggatccgc tggtggtttg agtaagattc aaaacctgtc ttacataatc cgcatccacg
      481 tcaggtccgc acaagaggag cgagctttgt gtcaaagaac cacctgtttt gtgcatgctg
      541 actgcagcca tgtcagcacc tacagccatt gcggatacag gcatgtcatc gccaaaatag
      601 aaatgggttc cgtgagcctc atccaccagt acttttacac ccttgtcctt tgccagtctt
      661 acaatttcct taagattcga gcaaataccg taatatgtgg gattgttcac aaataccgct
      721 tttgcatccg ggtgctcatc cagagctttc ttaacatctg cgacagacat tcctaaagga
      781 attccaagct ttgtacttat acctggatta atataaattg gttttgcacc aattaccacc
      841 agtgcattta ttgcacttct gtgcacattt cttgggatta ttatcttgtc acctgccttg
      901 caggtggaca ttatcatcgt ctgtacggca gacgaagttc cattcaccat gaaaaacgct
      961 tctcttgcag aaaacgcctc tgcgcacagc ttttgcgcct cctttataac tgaggatggg
     1021 tgaatcaggt tatcaagagg ttttctcgag ttgtaatcca ctctgagaca attctcaccc
     1081 agaaactctg ccagttctgg attgccacgt cctcccttat gtccgggcac atcgaatgca
     1141 actacccttt ccaccttgtg gctctttact gcttcaaata gtggtgctct attttgatcc
     1201 attgttgcct ctctccaaag attatttctg gtatatattc ataccactga aaatttcaat
     1261 catttctttt ctcaatctat ccgttatttc aagtctttgc tttggaggca attcatatac
     1321 atcctgatta aataaataat tctgcaaatc tatttcctta attagcattt tagtatgaaa
     1381 aatatttgcc tgataaacat ttacatcaat ggcatcatac atattcaagg tttcttcgtc
     1441 tatataatcc tggatggaat taatatcatg atcgatataa tattttttcc cttcaagatc
     1501 tcttgtaaag ccgcgaaccc tgtaatctat tgtaattatg tcggagtcaa agctctctat
     1561 taagtagttt agggctttta gcggagagac tcttccgcag gttgatacat ctatatcaac
     1621 cctgaaggtg gatattgcgt tgtcaggatg ggcttcagga tatgtgtgca ctgtaacatg
     1681 acttttgtcc aaatgcgcat gaattgtttc cttataccca cttattcctt ttcctaaatt
     1741 gcacgtttca tcaacttgcg attctgacaa ggctccttct gcaatcaata tgtttacaga
     1801 agccccttga ggctcataat cctgtttcga tatgttgagt atgtgcgcac ctataagttc
     1861 agtaactttg catagtatcg atgtcagcct ttcagagtta tactgttcat caatgtatgc
     1921 gatgtaatct ctttgctccc tatccgtttt tgcatagctt acatcataaa tattgaagct
     1981 aagagtcttg gtcaggttat tgaacccata caatgccaac ttttttgcca actctaacat
     2041 cacacctttc aaatataatg atacagctag aataataccc tatttccgtt caaaaaacaa
     2101 caaattaatt gtgtgatatt gttacagata ttagcgtttt ttgtgttata atatatcctg
     2161 aacaagaaat aatataataa agcagtaaca gctatattaa ttgtagtcaa taaaatgtaa
     2221 aggaggataa acatgagtgt aataacattg acaaaggata atttcgaaga tgaggtgttg
     2281 aattcagatg tcccggtttt ggtggatttc tgggcaagct ggtgcggtcc atgcagaatg
     2341 atttccccta tcatcgaaga aattgcagca gaggaaaatg gaatcaaggt tggcaaggtt
     2401 aatgttgatg acgagcctga acttgcatca acttataaca taatgagcat ccctacaatc
     2461 atggtattta aaaacggcga ggttgtaaat gtcgcagtag gtgccaggcc aaaatctgca
     2521 cttctctcac tggtaaaaag ctaatattat ggaaaacagc aaattcgggt tcgttcgtca
     2581 tccttacgag cccgttttta atattgattc taaagtacta attttaggga ctgttccatc
     2641 tgttgcgtcc cgcaaatacg gcttttacta tacccaccct caaaacaggt tttggaaggt
     2701 tttgtcttcc attttaaacg aagaatttcc cgatactatc gaaaaaaaga ctgacatgct
     2761 gttgaaaaac cacattgctc tatgggatgt gttaaaagaa acacaaatca aaggaagtag
     2821 cgacagctct attaaaaatc ccaaacccaa tgatatcaac cggattctaa acagcactga
     2881 catcaaagca atttttacaa cgggcaaaaa agcctttgaa ctttataaca aattatgtta
     2941 tcctaaaacg aagttcaagg cgatttgcct tccttccacc agtcctgcca actgtgttta
     3001 cacattggac aggcttattg atgaatactc aatcatacta aaatatatac agtaaggaat
     3061 gaatatgaaa gatattattt tagccagcaa aagcccgaga agaattgaac tgtttaaaaa
     3121 actaaatcta cctttttcat gcattacgcc ggacatagat gaagcaatgg atttatcctg
     3181 cggccttgaa aatgcggttg ccaagcttgc tttcagaaaa gctaattcca ttattgagaa
     3241 gcatcataac accattgtta tcggcgcaga tacaattgta acactggaaa atttcatcct
     3301 gggaaaaccg agagacaaac aggatgcctt caacatgctc atgcaactgt caggaaaaac
     3361 acacaaggta atgacagggg tatgcatatt gacagataat aggatggaat ccttctgtga
     3421 tacctccgag gtcactttct accacttgac agacaatgag attaaaaaat acattgcaac
     3481 aggcgaaccc atggacaagg caggtgcgta cggcatccag ggacaaggcg ccctgcttgt
     3541 aaaacacatt aacggagact tttactccat catgggtctt ccaattgcaa gaataaacca
     3601 aatcctgaaa tcctatacta ttgattaaat gttactttct ccacatcata catggatgaa
     3661 agtacaatcc aatttgctgc attgaaactg tttatagtcc agtagctcac accaccaaga
     3721 ccatactcat caaccagcct taatcttgcc tgta
//
LOCUS       DAONVY010000200         3752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17090_length_3752_cov_3.604544, whole genome shotgun sequence.
ACCESSION   DAONVY010000200 DAONVY010000000
VERSION     DAONVY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17090_length_3752_cov_3.604544"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1350)
                     /locus_tag="PLI11_06200"
                     /pseudo
     CDS             complement(77..1350)
                     /locus_tag="PLI11_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679861.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
     gene            complement(1516..2844)
                     /locus_tag="PLI11_06205"
     CDS             complement(1516..2844)
                     /locus_tag="PLI11_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPZ52491.1"
                     /translation="MKSAFKTAAVVFMMMAVVVGFTACGGYNIPHHTPHQYQIAVEGN
                     VTLSVKATTQKEMLSYHAFIKEFENTCPEASVSLDTLGGDLDARIASGDIGDVFVFNA
                     DRLSHLVETRTLIKLDLYASHLGFDFSNIYSAAYDAGTYSGGLYMVSVDYARLSLVAN
                     KDALSEEGLDLPSNDWTWREFIEDYAQKLVKINEDGAYSQVPMFLDLNWDLVYIAFME
                     GWGGKWYDTVNRKISLLSDEDVLSGITEMFEAADEGYLYPTGKITSDYDGLEKSDYVF
                     HQMLYSNLKDYGEEYDGLGVDWDIASFPLLPTPKVGTYITGAGVYCRTKRPDTAAVLA
                     LFFNTEEGQKAFHGEKGGGVPVFKTFEKSDFWRKRESSWEGKNMDAFICYPEYDTVGH
                     LSAKVPEGVADLIKTPWSLMLEQYFNGTKHFMDWLDEIEREASEYWQKLD"
     gene            complement(2866..3006)
                     /locus_tag="PLI11_06210"
     CDS             complement(2866..3006)
                     /locus_tag="PLI11_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52492.1"
                     /translation="MAFIIEESWEKMLKQHFDGCKHFVDSLYDMERKANELWGTLDEA
                     DS"
BASE COUNT         1101 a          892 c          612 g         1147 t
ORIGIN      
        1 atcaaaatgc gggttttatc agtacctaca aaaatacaat tgccactgct gccggaatac
       61 aggctggcaa attttatcat atcgggaatt cctcagcttt tactctatga atgtcagcac
      121 caagttgtgc aagtttcttt tcgaaaccgt cgtaacctct gtctatgtat ttaatattgt
      181 aaacatcgga tctgccttca gatgcaagag cggcaataac aagagcagca ccagctctca
      241 aatcggtgca tttgacaaag gtccctttgt atttcctgtt tcctgtaatg tatgcagtgc
      301 gtccctctac cttaatcatt gcacccattc gtttaagttc atctacatat tggaatctgt
      361 cccagatacc ttctgaaacc acactgtctc cgtctgcaag agacaataga gctgttatct
      421 gaggctgcat gtcggtggga aaaccaggat aaggcagtgt ctttacattt gcgccattca
      481 tcctgccttt gtgaaatact cttatgtaat cgtccccttc ctcaattccc acattcatct
      541 cctgtagttt tgcggtcaga gactcttgat gtcttggaat aatatttctc agtgtaacat
      601 cgccttttgt ggctgcagca gccaccatga aagttcctgc ttcaatctga tcgggaataa
      661 tagaataagt aataccgcct tttatctctt tgcatccctt gattctaatg gtatcagtac
      721 cagcaccttt aatatttgct cccatggcat tcaaaaagtt cgccacgtca accacatggg
      781 gctctcttgc acagttctca ataattgtat tgccttccgc cctgctggca gccagcataa
      841 gattgatggt tgcacctact gtaacgccgt caagataaat actcgttcct ttaagtccct
      901 ttttagcgtt ggtctttacc ataccgtatt cttcaacggt atccgcacca agtgcattga
      961 aacccttcaa atgaaggtca attggtcttg gacctaagtt gcatcctccg gggagggata
     1021 cttcagcttt tccgaaacga cccaaaagcg ctcctatgaa atagtaggaa ccccttaaag
     1081 tacttatgga ttcgcttatt gccttgtggc ttttaacgtt acggggatca atcttgataa
     1141 cattcttgct catccaggcg catttagctc ccaatgcaat agcatatcaa caattatctt
     1201 gacatccagt atgtcaggta cgttttcaat aatacaggga ttttctgcta aaagtactgc
     1261 aggcaaaatc cccaatgtag catttttgct tccactaaca gttatatctc catttaatgg
     1321 ctttccgccg tatattataa acttctccaa atttcacacc tactcatgat ttcttactct
     1381 taacttctct tttataacca catattgagt aatcctactg caacgcctac agtatatcat
     1441 accataaagt ttgtttcaat gagaagtaaa gtataaatca taagttgatt gaaattttac
     1501 aattaaattc tataactaat caagcttttg ccaatactca ctggcttccc tttcaatctc
     1561 atcaagccaa tccataaaat gtttggtacc attaaagtac tgttcaagca taaggctcca
     1621 tggtgtcttt atcaaatccg ccacaccttc aggaaccttt gcacttaagt ggcccaccgt
     1681 atcatactcc ggataacaaa taaaagcatc catgttcttt ccttcccagg aagactctct
     1741 ctttcgccag aaatctgact tttcaaaggt tttaaaaaca ggcactccgc cgcccttttc
     1801 accatgaaaa gccttctgcc cttcttcagt attgaagaac agagccaaaa ctgccgccgt
     1861 gtcgggtctt ttggttctgc agtacacacc agcccccgtg atgtaagttc caacctttgg
     1921 agttggtaat agcggaaaag acgctatatc ccaatcaact cccagaccat catactcctc
     1981 tccataatcc ttaagatttg aatacagcat ctgatggaat acataatccg atttttcaag
     2041 accatcatag tcggaggtga tttttccagt aggatacaag taaccttcat ctgctgcttc
     2101 aaacatttca gtgataccgg agagaacatc ttcatcagac aggagactga tcttcctgtt
     2161 aacggtatca taccatttgc cgccccaacc ttccatgaag gcaatataaa ccaagtccca
     2221 gttcaagtcc agaaacatgg gaacttgtga atatgcacca tcttcattta ttttaacaag
     2281 tttttgtgcg taatcctcta tgaactctct ccatgtccag tcattggatg gcaaatcaag
     2341 tccttcctca gaaagagcat ccttgtttgc cacaagcgaa agcctggcat aatcgacact
     2401 caccatgtac aatcctccgc tatacgttcc tgcatcgtaa gctgctgaat aaatattgct
     2461 aaaatcaaaa cccagatgtg aagcatatag atcgagcttt atcaatgttc ttgtttctac
     2521 caaatgggaa agcctatcag cattaaacac aaaaacatca ccaatgtctc ctgatgcaat
     2581 ccttgcatcc aaatctccac caagggtatc cagcgaaacg gatgcctcag ggcatgtatt
     2641 ttcaaattcc ttaataaaag catgatatga cagcatctcc ttctgagttg tagccttaac
     2701 tgacaaggtc acgttgcctt ccacggcaat ctgatactga tgcggagtat gatgcggtat
     2761 gttatagcct ccacaggctg taaatccaac aacaaccgcc atcatcataa acacaactgc
     2821 agctgtcttg aaagcacttt tcataaaaaa ctcctttcaa aataattatg aatccgcctc
     2881 gtcaagagtc ccccaaagct catttgcttt gcgttccatg tcatatagcg aatctacaaa
     2941 gtgtttgcaa ccatcaaagt gctgttttag cattttttcc catgattctt cgattatgaa
     3001 tgccacttcg gaaggaatcc tcccctttat tattccaatc gtatcgagtt caggaaactt
     3061 acaaatgctg taccaaaacc ataaacacaa caagtcctgt ttttacagta cgtttcatat
     3121 gaaagactcc cctcaagagt gtttgttcgg ttattactac catcatttta taccactgcc
     3181 ttgtttcagg tcaatgcaca acccctgctt tttttaatat ttgactaaaa atttacatta
     3241 tgtaaacctt tgtgcaataa ataaaaagag ctaagcaatt agacgaagtt acaggtatga
     3301 gtctattatg gattccttat caaatataaa gtaaggtcgt cattaaccgg atcgattgaa
     3361 ccagtcccgt cgtcatatac attggtatgc tctgaatcag tgtataatgt aaaattatca
     3421 taatacttca cactaaacat tgaatctttt tgaatttcaa atttatcaaa caaagcattc
     3481 ccatttcgca tttcaattat tgtaaccgta acagtagtgc caaattcata atctcttacg
     3541 aaacttggga agtcatgacc atatgattaa agaagtcatt aacaaggaaa ttttccgtac
     3601 cactccaccg ttagagatca agaaatttct gctgactttt tacttttagg aattgcctta
     3661 ctaagatgac tgctacaagc gcaataaaca ataaaattgc tataaaagtt acgattgctt
     3721 tggtcagttg acctgagttg acatcataca at
//
LOCUS       DAONVY010000201         3750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17098_length_3750_cov_3.261976, whole genome shotgun sequence.
ACCESSION   DAONVY010000201 DAONVY010000000
VERSION     DAONVY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17098_length_3750_cov_3.261976"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLI11_06215"
     CDS             <1..237
                     /locus_tag="PLI11_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52493.1"
                     /translation="VLMLRKKLGARNISFPIDDTFILGARVKMHADNIHEILLEQSLK
                     EEPYIISEKILIKLGVELRKFDDAMFDYKGLTGS"
     gene            259..1086
                     /locus_tag="PLI11_06220"
     CDS             259..1086
                     /locus_tag="PLI11_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52494.1"
                     /translation="MVKREVIELLNVKKERFFDEYCNVDFSKYEIYVEFCHNEQIQDR
                     IKEISQVKGEDKLSLEELNERTTFLRSVSLVYEDCAYVLVTNRPYSTEKYFTFALYSE
                     LAKVYAIHKGEYDRNRIGEDEFEIDSMMGYAFWNAFASRMIAYELMSKEYPEVKKICQ
                     RKTLISENIQFIDVDIRKRHLGCVLLADLYAICVVQSRLRARNIDADKYSFPVDKRWY
                     VGKKLHKELAVLLKLIVNQLNKDEPYSIDLAALEKIGSSVYDIFWKLKEYEATFAVV"
     gene            complement(1083..2657)
                     /locus_tag="PLI11_06225"
     CDS             complement(1083..2657)
                     /locus_tag="PLI11_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52495.1"
                     /translation="MLDRSLQNCEIVSNINMERKEDFEFLCNHIVLCIRTNNKPNRLY
                     TNFVNTHTGNIKQKEKVDEREAPLRHAGFPEKEGHLANGQRDAINNLNFMKSGEFFSV
                     KYANTDIKLSTIKSMVFNNLIQSALGSGPAPITVYAGNDETIKDLLAYMNMQDEGNTL
                     CKRWLEGINTIATYVNDKYDAREFQAITKDGKGFITKKDEMVAIDDMKQKFMYNCFLL
                     FDRNFTDLTTCRDLLQTRLVLLEDAKKRIIEASQNLNFDKSPQELKRKLDEDISKFTD
                     KQNKLNARLNEWKEYFNSLPKGLKFLVKKKIQEKFEGFVQDEEKDIFSSDSTYEDVEK
                     HYNDKIQTVKNGLDDYKKDKKYAFDVLDALDALKNLGIDIENKLTSLTLDEVNEILDS
                     TVRYHQVWYALHYYECRWLMGEYSLKDVYKHLVTKDVIERRYRRLSMITPLLVMNFED
                     VPYNFFAYVSEDKPISPITDFMDLLILDNADTIPAGVGANVFFLAKQAVVFGKEEKLN
                     DSVFDVSSKSCIVNID"
     gene            2778..3674
                     /locus_tag="PLI11_06230"
     CDS             2778..3674
                     /locus_tag="PLI11_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPZ52496.1"
                     /translation="MSKSGKFLGIGMKAVKPIINSLGLNKRRKGHNLLGVIKGVPKEV
                     GLSPAPDFPCQAEWIIPKEENSAKIILFVHGGAYLSGSIISSRPVAGLLCQVTESRVL
                     TFEYGLAPENPFPRALEDAVKVWKHLTKDYNPKNIVVVGESAGGGLALALGLYLKDNN
                     MELPIGMVTLSAWADLTITNESHKTLDKKDPILSSDELRIAALKYSHGEYLKNPYISP
                     VYGDFTGFCPVLVHVGSDEVLLDDSKILYERLKEANVEAKLSIFEGMWHVWHGFEVPE
                     SIEALNEIKEFMEGLSDGEEMV"
BASE COUNT         1102 a          669 c          822 g         1157 t
ORIGIN      
        1 gttcttatgt tgagaaagaa acttggtgca aggaatatct cttttcccat agatgacacc
       61 tttatacttg gtgcacgagt caaaatgcat gcggacaaca tccatgagat actgctcgaa
      121 caaagtctca aggaagaacc atatatcatc tctgagaaga tattaataaa acttggagtt
      181 gagctgagaa aatttgatga tgccatgttc gattacaagg gtctgacagg ttcttgatta
      241 tgaagggagt gaagaattat ggtcaaacgt gaagttattg agctgttgaa tgtcaaaaag
      301 gaaaggttct ttgatgagta ttgcaatgtt gacttttcga aatatgaaat atacgtggag
      361 ttttgtcaca atgagcagat acaggacagg ataaaagaga taagccaggt taaaggagag
      421 gacaagttgt cccttgaaga acttaatgag cgtactacat ttttgaggag cgtttctttg
      481 gtgtatgagg actgtgctta tgtgctggtt accaacagac cgtactcaac ggaaaaatac
      541 tttacctttg ccctgtattc ggaacttgcg aaagtgtatg caatacacaa gggagagtat
      601 gacaggaata gaatagggga ggatgagttt gaaattgact ccatgatggg ttatgctttc
      661 tggaacgcat ttgcatcgcg gatgattgca tacgagctta tgagcaagga atatcctgag
      721 gtaaaaaaga tatgccagag aaagacattg ataagtgaga atattcaatt cattgacgtg
      781 gatattagaa agagacatct tggatgcgtt ttgcttgcag acctttatgc aatatgtgtt
      841 gtccagagca gattgagggc aaggaatatc gatgcggaca aatactcctt tcccgtggat
      901 aaaagatggt atgtgggcaa gaaactgcac aaggaattgg cagtactgtt aaaattaatt
      961 gtaaatcaat tgaataagga cgagccttat tcaattgatt tggccgctct tgagaaaatt
     1021 ggaagcagcg tatacgatat tttctggaaa ctaaaagaat atgaagccac ttttgcagta
     1081 gtctaatcaa tattcacaat gcagcttttg cttgatacgt caaacacgct gtcgttcaat
     1141 ttttcttctt ttccaaagac aactgcctgt ttggcaagga aaaagacatt tgcgccaact
     1201 cctgcaggaa ttgtatccgc attatccaga atcaacagat ccatgaagtc ggtaatgggg
     1261 gagatgggtt tgtcctccga tacataggca aagaagttgt agggaacatc ctcaaaattc
     1321 attacaagaa gtggtgtaat catggaaaga cggcgatatc ttctttctat aacatctttt
     1381 gtaaccaaat gcttgtaaac gtctttgagg gagtattctc ccataagcca cctgcattcg
     1441 taataatgga gggcatacca tacctgatga tagcggactg tgctgtcaag aatctcattt
     1501 acttcatcca gtgttagact tgtcagctta ttttcaatgt caattccaag gtttttcaag
     1561 gcatcaagtg catctaacac atcgaaagca tactttttgt ctttcttgta gtcatcaagc
     1621 ccattcttta cagtctgtat cttgtcattg taatgttttt ctacatcttc ataggttgag
     1681 tcggatgaaa agatatcttt ttcttcatcc tgtacgaaac cttcaaattt ctcctgaatc
     1741 tttttcttca caaggaattt aagtcctttt ggaagagagt tgaaatattc cttccattca
     1801 tttagtctgg cattcaactt gttttgcttg tcagtgaact ttgaaatatc ttcatcaagt
     1861 ttccgtttaa gttcctgtgg tgatttgtca aagttcaaat tttgggaagc ttcgattatt
     1921 cttttctttg catcctccag aaggacaagc cttgtctgaa gcaggtcgcg gcatgtggtc
     1981 agatcggtaa agttcctgtc aaaaagcagg aaacagttgt acatgaactt ctgcttcata
     2041 tcatcaatgg cgaccatttc gtccttttta gtaatgaagc cttttccgtc ttttgtaatc
     2101 gcctggaatt cacgagcatc gtacttgtcg tttacgtatg tggcaatagt atttattcct
     2161 tccaaccatc ttttgcacag cgtgtttccc tcgtcctgca tgttcatata tgccagtaaa
     2221 tcttttatgg tttcatcatt gcctgcatag actgtgatag gggcaggacc gcttcccaga
     2281 gcggattgaa tcaagttgtt aaacaccatg gatttgatgg tggaaagttt tatatcggta
     2341 tttgcatatt taacagagaa aaactctccg cttttcataa agttcaaatt gtttatggca
     2401 tccctttgtc cgtttgcaag atgaccttcc ttttctggga aacctgcatg cctcaatggg
     2461 gcttcccttt cgtccacctt ctccttttgc tttatattcc ccgtatgggt gtttacaaag
     2521 tttgtataca gcctgtttgg cttgttgttg gttcttatgc acaggacaat atggttgcaa
     2581 agaaattcaa agtcttcctt tctttccata ttgatatttg aaacaatctc gcagttttga
     2641 agagatctgt ccagcatttt tcatacctcc gaaaatattt gtataaacat tatatactct
     2701 gattaaggca aatacaatat gtatgaattt agtttgtatg gtataatata gtgaggagtt
     2761 gaaaggggat taagaaagtg agcaaatcag gaaaattttt aggaataggc atgaaagctg
     2821 taaaaccaat aataaacagc cttggcttga acaagaggag aaaaggacac aatcttttgg
     2881 gagttataaa aggtgtgcca aaggaagtag gactatctcc ggctccggat ttcccgtgtc
     2941 aagctgaatg gattattccc aaggaggaga actccgctaa aatcatttta tttgtacacg
     3001 gaggggcgta tttgagcggt tcgattataa gctcaagacc tgttgcaggt ctcctatgcc
     3061 aggttactga aagccgtgtt ttaacttttg agtacggact cgctcctgaa aacccctttc
     3121 ccagagctct tgaagatgcg gtgaaggtgt ggaagcattt gacaaaagat tataatccta
     3181 aaaacattgt ggttgtggga gaaagcgcag gagggggtct tgcccttgct cttgggcttt
     3241 atctaaagga taacaacatg gaactcccta taggcatggt tacattgtcc gcctgggcgg
     3301 atctaaccat aacgaatgaa tcccacaaaa ccttggataa aaaagacccg attctttcca
     3361 gcgacgagct cagaattgca gcgctcaagt acagtcatgg agagtacttg aaaaatccat
     3421 acatatcacc cgtatatggt gattttacag gattctgtcc tgttcttgtt catgtaggtt
     3481 ccgatgaagt gctgcttgat gattcgaaaa ttttgtatga aaggcttaaa gaggcgaatg
     3541 tggaagcaaa gctcagcata tttgagggaa tgtggcatgt atggcacggt tttgaagtgc
     3601 ccgaatcaat agaagcattg aatgaaatca aggagtttat ggaagggttg tccgatggag
     3661 aagaaatggt atagacttga taacgctgca aaaatttttc ctgttacaaa aaattcaatg
     3721 tggtccaaca attttcgagt atcagtaact
//
LOCUS       DAONVY010000202         3737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17178_length_3737_cov_3.502988, whole genome shotgun sequence.
ACCESSION   DAONVY010000202 DAONVY010000000
VERSION     DAONVY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17178_length_3737_cov_3.502988"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PLI11_06235"
     CDS             complement(<1..1397)
                     /locus_tag="PLI11_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPZ52497.1"
                     /translation="MEPASKDKKLRIHSIYIKFLAIFTITIVLSSVLSGTIMYKLVES
                     YLIASRMDDLKSAADTISDYVTQYLTSDEYVGEFVPVEHDKNEYFYNLYSLMKISNQT
                     MGANIFITDDKGYVGFSYPLLPDMADKRIEHGSRFLNDSIVANLILDNGRYRFPNKEQ
                     YVPSLRTDGYVVDKNHYHGLFRDEKVPFLTISRRLVYIEPETRIKQVYGTVCISVSTP
                     EILEARQKVILYFMLSTCIAVLIQVIVLSISTKRITEPVRALQEASRRLAAGSFERNV
                     IRTTKDEIGDLVDSFNNMTIALENLDRVRNDFIANVSHELRTPMTSIGGFIDGIMDGV
                     IPPEKHEYYLKIVRSEISRLSTLVNELLDIAKMQSGNMEFHFTVFDINVDIRNCIIKL
                     EPLINDKELQVELDLDNEQENVYGDRNSLQRVLINLIQNAIKFTPKGGTIKVYTKRLK
                     DKVEVNVEDTGIGIS"
     gene            complement(1400..2095)
                     /locus_tag="PLI11_06240"
     CDS             complement(1400..2095)
                     /locus_tag="PLI11_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPZ52498.1"
                     /translation="MAQYSKVKILVVDDDENICELVRLYLDKEGFEVSTANDGIEAIE
                     EFKRTTPDMVILDIMLPGADGWQVCREIRKVSQIPIIMLSAKGDTFDKVLGLELGADD
                     YLVKPFEPKELIARVKAVMRRSGGSEKDTQEEVVYPNLLVNKTTYTVKVDGKEVQMPP
                     KELELLYFLSSNPNKVFTRDQLLEYIWGYDFFGDSRTVDVHIKRLREKIEHPSHPWSI
                     KTVWGIGYKFEVK"
     gene            2258..3352
                     /gene="ychF"
                     /locus_tag="PLI11_06245"
     CDS             2258..3352
                     /gene="ychF"
                     /locus_tag="PLI11_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPZ52499.1"
                     /translation="MKLGIIGLPNVGKSTLFNAITGQGAESANYPFCTIDPNVGVVAV
                     PDPRLDVLQKMFNSEKLTPAHIEFVDIAGLVKGASKGEGLGNKFLSNIREVDAIIHVV
                     RCFDDENIIRVDGSANPKTDTETINFELIFSDIEMLERRIDKTQKMSKSGDKALLKEL
                     ELLNRLKEHLESGKSARTFPRTEEEEEIISTIYLLSDKPVIYMANISEDDIMDPENNN
                     HVRELKKIADDENAELIVVCSKLEEEISALSKEEKEEFLKELGIEESGLDQLITSSYR
                     LLGLISFLTAGPKEVRAWTIKKGTKAQKAAGKIHSDIERGFIRAEVIPFEELVAVGGM
                     TAAKEKGLVKSEGKEYIMQDGDVTLFRFNV"
     gene            3400..>3737
                     /locus_tag="PLI11_06250"
     CDS             3400..>3737
                     /locus_tag="PLI11_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52500.1"
                     /translation="MKLAIVGYGGMGAWHHENIRARIPEIEVVGAYDIREERQEEAKK
                     QGLRTYNTFDELVSDDSIDIVTIATPNNFHKKQAIRCLESGKHVICEKPVTMNADELE
                     EIIEVAKKCN"
BASE COUNT         1170 a          748 c          713 g         1106 t
ORIGIN      
        1 tttgaaatac ctattccagt atcctctaca tttacctcaa ccttgtcttt taatctcttt
       61 gtatatactt ttatcgttcc accttttggt gtgaacttga tagcattctg aataaggttg
      121 ataagtactc tttgcaagga gtttctgtct ccataaacat tctcctgctc attgtctaaa
      181 tccagttcaa cttgcaattc cttgtcattg ataagcggct ctagcttgat aatacaattt
      241 ctaatatcaa cattaatatc aaacaccgta aaatggaatt ccatgtttcc agactgcatt
      301 tttgcaatat caagaagttc atttactaga gtactgagtc tggatatttc acttcttaca
      361 atctttaaat aatactcatg cttttcagga ggtattacgc catccattat tccgtcaata
      421 aatccaccta tagaagtcat gggtgtacgc aattcatggg atacatttgc tatgaagtcg
      481 tttcttaccc ggtcaagatt ctctagagct attgtcatgt tgttaaaact gtctacaaga
      541 tcgcctattt catccttcgt tgtccttatg acatttctct caaaagaacc tgccgcaaga
      601 cgcctcgagg cttcttgcaa cgcccttaca ggttccgtaa tcctctttgt tgatatggaa
      661 agaacgatga cttgtatcaa gaccgcaata catgtggaca gcatgaagta cagtataacc
      721 ttttgccgtg cctccaaaat ctcaggagtg gatacggata tgcaaaccgt gccatatacc
      781 tgcttgattc tggtttccgg ctcaatgtat acgagtcttc tgctgatagt tagaaatggg
      841 accttttcat ccctgaaaag tccatggtaa tggtttttgt caacaacgta accatctgtt
      901 ctcaagctgg gtacgtactg ttctttgttt ggaaagcgat atctgccgtt gtcaaggata
      961 agatttgcca ctatgctgtc atttaaaaat cgactgccat gctctattct cttgtctgcc
     1021 atgtcgggta aaagagggta tgaaaaacca acatagccct tgtcgtcggt tatgaaaata
     1081 ttggccccca ttgtctggtt gcttattttc atcaggctat acaggttgta gaagtattcg
     1141 tttttatcgt gttctacggg aacaaattcc cctacatact cgtcactggt cagatactgg
     1201 gtaacgtaat ccgatatggt atccgcagcg cttttcaagt cgtccattct gcttgctata
     1261 aggtaactct caacgagttt gtacattatt gttcccgata gaaccgatga caatacaatc
     1321 gtaatagtga aaatggcaag aaacttgata tagatcgagt ggattctgag tttcttatcc
     1381 tttgacgcag gttccaaatt tatttcacct caaatttata accaattccc cacactgtct
     1441 taattgacca tggatgagat gggtgctcaa ttttctcgcg caatctcttt atgtgtacat
     1501 ccaccgttct ggaatcccca aagaaatcat atccccagat gtattcaaga agctgatctc
     1561 ttgtaaacac cttatttgga tttgaggata aaaagtataa caactcgagt tctttaggag
     1621 gcatctgtac ttcttttccg tctactttta cagtgtatgt ggtcttgttt accaacaggt
     1681 ttggatatac aacctcttcc tgggtatcct tctcggaacc acctgaacgc ctcattaccg
     1741 cctttactct agctatgagt tcctttggct caaaaggttt tacaaggtaa tcgtccgctc
     1801 ccaattcaag cccaagaacc ttgtcaaagg tatcgccttt tgctgataac atgattatgg
     1861 gtatctggct tacttttcta atttccctgc atacctgcca gccatccgca cctggaagca
     1921 tgatgtccag tattaccatg tcaggagtgg tccttttgaa ttcttcaatt gcttcgatac
     1981 cgtcgttagc tgtacttact tcgaaccctt ctttgtctag atataaacgg accagttcgc
     2041 agatattctc gtcgtcatca actactaaaa ttttaacttt gctgtattgt gccatgtaaa
     2101 agcctccttt aaaaattctt aatttattag tgattattta aaaatatagt attaacatat
     2161 tatactatat taatttaaaa atatagtatt aacatattat actatattaa ttaaataata
     2221 ctatacaaca ttataccatg aaagaaggaa ttttaccatg aaattaggaa ttataggttt
     2281 gcccaacgta ggaaaaagca cactttttaa tgcaataaca ggacaaggag cagaaagtgc
     2341 caactatccc ttttgcacca tagatccaaa tgtgggtgtc gttgctgttc ccgaccccag
     2401 attggatgta cttcaaaaaa tgttcaactc agaaaaacta acaccggcac atattgaatt
     2461 cgttgatatt gcaggacttg tgaaaggcgc cagcaaagga gaaggacttg gcaacaagtt
     2521 tctttccaac ataagggaag tggatgcgat tatccatgta gtaagatgct ttgacgatga
     2581 gaatattatc agagttgacg gttcggcaaa ccctaaaacc gatacggaaa caataaattt
     2641 tgaacttatt ttttcagata ttgaaatgct tgaaagaaga attgacaaaa cccaaaagat
     2701 gtcaaaaagt ggagacaagg cactacttaa agaacttgag ctgttaaaca gacttaagga
     2761 acaccttgaa agcggtaaaa gcgcaagaac atttccaagg acggaggaag aagaagaaat
     2821 aatatccacc atttatctac tctcggacaa gcccgttatc tacatggcaa atatatctga
     2881 agatgacata atggacccgg agaacaacaa ccatgttaga gaattaaaga aaatcgccga
     2941 tgatgaaaat gctgaactga ttgttgtatg cagcaagctg gaagaagaaa tttcagctct
     3001 ttccaaggaa gaaaaggaag aattccttaa agaactgggt attgaagaat ccggcctcga
     3061 ccaactcata acttcaagtt acagacttct tggcctcata agtttcctta cagcagggcc
     3121 gaaggaagtc agggcatgga ccataaaaaa aggaacaaaa gctcaaaaag ctgctggaaa
     3181 aatccacagc gacatagaaa gaggctttat tagagcagag gtcattccct ttgaagaact
     3241 ggtagctgtt ggaggtatga ctgctgccaa agaaaaagga cttgtcaaat ccgaaggaaa
     3301 agagtatatt atgcaagatg gcgatgtaac actgtttagg tttaatgtgt aaaataccat
     3361 gtagtttcaa ataattataa taaattttgg agggatttaa tgaaacttgc tattgttgga
     3421 tatggaggaa tgggcgcatg gcatcatgaa aacattagag cccgcattcc ggaaatagag
     3481 gttgtaggcg catatgatat aagggaagaa agacaggaag aagccaaaaa acagggtttg
     3541 cgcacataca atacttttga tgaacttgta agcgatgact ctatagacat tgtcaccatc
     3601 gcaacgccaa acaacttcca caaaaaacag gcaatcaggt gcctcgagag tggaaagcat
     3661 gtaatatgcg aaaaacccgt taccatgaac gccgatgaac tggaggaaat catcgaggtt
     3721 gcaaaaaaat gcaacaa
//
LOCUS       DAONVY010000203         3714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17316_length_3714_cov_2.968297, whole genome shotgun sequence.
ACCESSION   DAONVY010000203 DAONVY010000000
VERSION     DAONVY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17316_length_3714_cov_2.968297"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLI11_06255"
     CDS             complement(<1..400)
                     /locus_tag="PLI11_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPZ52501.1"
                     /translation="MKMLKVYDLMKYIEEIAPSEKALSFDNVGLMTGSRDKEVRGILC
                     CLDLTREVIYEAVEEGVNTIITHHPFIFDPVKRLDEDNVRGKLLGMAVRYELNVLSAH
                     TNWDFAEKGVNHALCVKIGLKDIREDEYMQH"
     gene            complement(378..1082)
                     /locus_tag="PLI11_06260"
     CDS             complement(378..1082)
                     /locus_tag="PLI11_06260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPZ52502.1"
                     /translation="MVLRNKRLLMVANMIPKCECMADIGTDHALLPIYLVLNGKCNRA
                     IASDVKIGPLQSAEKNIKRHHLSDKIETRLGSGFEVLKTGECQVSVLAGMGGILISRL
                     IEDSFDIAKETPYLVLQPNTEEASLRKYLFDNGFEILDENAVMDRGHCYLVILCRHVP
                     GLVSEYDEIDLIVGKVLNKKDTEGRVVYYQELRQKADKVLRKLEKVKNRTEEIEKRIV
                     EYKKVVRWIDENVEGI"
     gene            1239..2170
                     /locus_tag="PLI11_06265"
                     /pseudo
     CDS             1239..2170
                     /locus_tag="PLI11_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185903.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(2211..3497)
                     /locus_tag="PLI11_06270"
     CDS             complement(2211..3497)
                     /locus_tag="PLI11_06270"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPZ52503.1"
                     /translation="MSKENYNAYEHAQAQFNSVADKIGLDEATCELLRQPIREYHFTI
                     PVKMDDGSTKVFKAFRIQHNDARGPAKGGIRFHPNETVDTIRALSMWMTWKCAVVDIP
                     LGGGKGGVICDPRTMSEREQEQLCRGYIRQLAKNIGPNIDVPAPDVMTNAKHMLWMLD
                     EYETIVGGRYPGVITGKPVGMGGSLGRTEATGFGVVFVLREALKALDIDITKTTASIQ
                     GFGNVAQYAALLYTQLGGKVIAISCWDNNDKTAYTFKNADGLDIEKLRSITDSFGTVD
                     KDKAKEMGCEILPGDAWIEQEVDILIPAALENQIKADNVHKISEKVKVLAEAANGPTT
                     PEADEVIKERGIFVIPDFLANAGGVTCSYFEQVQCNMNYFWEKEEVMQKLDTKMTAAF
                     KAVYDLHKEQDLYMRDAAYVISINRVAEAVKKRGWV"
BASE COUNT         1027 a          890 c          590 g         1207 t
ORIGIN      
        1 tgtgctgcat atattcatct tctcgtatat ccttaagccc aatttttaca cacaaggcat
       61 gattcacacc cttttctgca aaatcccaat ttgtatgagc agacaataca ttcaattcat
      121 acctgacagc catacccagc agttttcctc ttacattgtc ctcatcaagt cttttgacag
      181 gatcgaaaat gaagggatga tgtgtaatta ttgtgtttac accttcctct accgcttcat
      241 aaataacttc cctggtaaga tcaaggcagc acagaatgcc tctaacttcc ttgtccctac
      301 tgcccgtcat aaggcccaca ttgtcaaagg acaaggcctt ctcacttgga gcaatctctt
      361 caatatattt catcaaatca tataccttca acattttcat caatccacct taccaccttt
      421 ttatactcaa caatcctctt ttctatctct tccgtcctgt tttttacctt ttcaagcttt
      481 ctcagcacct tgtcagcctt ttgtctcaat tcctgatagt atactactct gccttcagta
      541 tcctttttat tcaaaacctt gcccacaatc aaatcaattt cgtcatactc actgacaagg
      601 cctggaacat gtctgcatag aataaccaga tagcaatgcc ccctgtccat aaccgcattc
      661 tcatccagta tctcaaagcc attatcaaac aagtacttcc tcaatgaagc ttcttccgta
      721 tttggctgca gtaccaaata gggtgtttct tttgcaatat caaaagaatc ctcaatcagc
      781 ctggatataa ggattccacc cataccggca agcactgaaa cttgacactc tccagttttc
      841 agcacttcaa atccagaacc caaccttgtt tcaattttat cacttaaatg gtgtctcttt
      901 atgttctttt cagcagattg cagcggacct atcttcacat cagaggcaat tgctctgttg
      961 cactttccat tcaagacaag atatatcggc agtagagcat gatctgttcc tatgtccgcc
     1021 atgcactcgc attttggtat catgttggct accattaata gtcttttgtt tctaagtacc
     1081 atcaatcaac ctccaaatat actaacaaaa catattatat ctacttttct ctcttgagta
     1141 aaggaatcag atttatggga caaagtcaca caaataatat tgatactcaa aaaaatgtgg
     1201 tatactttta gctcaaacca tatatatggg agtaagctat gaggaaaaga caagatcatt
     1261 tatttgcata catatcagca acactatacg cagcaatagt tagcatgtcc tttctaacag
     1321 taaagattgg ccttgtgagt gccaatcctt ttgagttgct tgcctacaga ttcactattg
     1381 cattccttgc aattgtgcta gcccttgcat tcagagtggt caaactaaga ataaccaagg
     1441 agaagatact aaaacttgct cctcttgcat tgttttaccc ggtattctac ttttccttcc
     1501 aaagctttgg acttacttac acaaccagtg tggaagcagg aatcatgaat gcactgattc
     1561 ctgtactgac acttatactt gctactattt tcctaaaaga aaaaactaca gttcttcaga
     1621 aactgtcaat aatactttct gtactaggag ttgtatacat aaccttaatg aaaggaaaca
     1681 gctcatcctc tcataacctg gcaggcataa tacttctttt tatttcctca acatcctttt
     1741 cagcttacag tgtgctggtc aagaagcgct caaaggagtt ttcgaccact gacataagtt
     1801 ttgtagtgac catattggga tttgtatcct ttaatctgct aaacttttgc aatcacttgc
     1861 taaaagggac attatccagt tgctaaaagg gacattatcc agctattttg aacctttgaa
     1921 aaacacaagt tttatatggt caattcttta tttaggaata ctctccacct tgttcacttc
     1981 actgcttgcc aactactcac tgtcaaaaat tgaggcttca aagatgaatg tttttgtaaa
     2041 cctggcaacc gtattgtcaa tagcagcagg ctctgttttt ctggatgaaa aaatatatta
     2101 ctatcatata atcggttctg ttttgattgt tttgggggtt ttgggaacta attttcttgg
     2161 aaaacggtag aaaaaaagag gctgcaaacc agcctccgca atactaaata ttatacccag
     2221 ccacgtttct tgactgcttc tgcaactcta tttattgata taacgtatgc agcgtcacgc
     2281 atgtaaaggt cctgctcctt gtgcagatca tatactgcct tgaatgctgc tgtcatcttt
     2341 gtatcaagtt tctgcataac ttcttccttc tcccagaaat agttcatgtt gcactgaacc
     2401 tgttcgaagt agctgcaggt aactcctcct gcatttgcca agaagtccgg aattacaaaa
     2461 atacctctct cttttataac ctcatctgct tccggtgttg taggtccgtt tgcagcttca
     2521 gcaagcacct ttaccttctc actaatttta tgaacgttgt ctgctttgat ttgattctca
     2581 agagcagcag gaataaggat atccacttcc tgttcaatcc atgcatctcc aggcagaatc
     2641 tcacaaccca tttctttggc cttgtctttg tcaactgtac caaaggaatc tgtgatgctg
     2701 cgcaactttt ctatgtcaag tccgtctgca ttcttgaatg tatatgcagt tttatcgtta
     2761 ttgtcccaac aggatattgc gattaccttg ccacccaact gtgtatacag caaagctgca
     2821 tactgtgcaa cattgccaaa accttgtatg cttgcagtgg tttttgtaat atcaatatcg
     2881 agagctttca aagcttctct gagtacaaat acaacaccaa atcctgttgc ttctgttctt
     2941 cccaatgaac cacccattcc aacaggcttt ccggtaatga cgccgggata tctgccgcct
     3001 acaattgtct catattcatc caacatccaa agcatgtgct ttgcatttgt cataacatca
     3061 ggtgcaggta catctatgtt aggtccaata ttctttgcca actgccttat atatccgcga
     3121 caaagctgtt cctgctcacg ttcagacatt gttctgggat cacatatgac gccgcctttt
     3181 cctccgccca gcgggatgtc aacaacagca catttccagg tcatccacat ggacaatgcc
     3241 ctaatggtat caacagtctc atttggatgg aaacgtatac ctccctttgc aggtcctctg
     3301 gcatcgttgt gttgaattct aaaagccttg aatacttttg tactgccatc atccattttt
     3361 actggtatgg taaaatgata ttctctaatt ggctgacgca acagttcgca agtcgcttca
     3421 tcgagcccta ttttatctgc cacactatta aactgcgcct gcgcatgttc atatgcgttg
     3481 tagttttctt tgctcattcg agtgttcccc ctatagttgt tttttcgtat gcaattatac
     3541 aatcatacgg atggcatgtc aacgaattta ctatgataaa tcgttaaaag ataactcgct
     3601 gctcaaacct tctggcagtt tcatcactgt ccagcaaata atattttacc aaccatatac
     3661 tcgccatata cgtcttgctt gaaatttgca aaggttagcg ctacatcaac cata
//
LOCUS       DAONVY010000204         3702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17381_length_3702_cov_4.230875, whole genome shotgun sequence.
ACCESSION   DAONVY010000204 DAONVY010000000
VERSION     DAONVY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17381_length_3702_cov_4.230875"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..3679)
                     /locus_tag="PLI11_06275"
     CDS             complement(50..3679)
                     /locus_tag="PLI11_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HPZ52504.1"
                     /translation="MAVIKASACNNDGRRKVGFIAPSIEGQKEVIETVFEMSSIPPHS
                     ISYVETHGTGTNVGDPIEIEALKQAFGIEGIDTPRCTIGSVKANIGHLHAAAGVASFI
                     KVVLSMNKGKIPPQINYTQPNQKIKFFEKCFNVNTTPVEWKKENAILRAGISSFGVGG
                     TNVHLILEEAPKHAEKEKDAKHSFDRFLLIMSAKSAFSLEQMTNNMANFLEKENEINM
                     ADAAFTLQMGRRQYNYRKICIASDAQDGAQALKSNDAQKVFSTYQEYSCRPVAFMFTG
                     QGSQYAGMIGDLYERSCFVREQVDMMAEIVSCKLGFDIRNYLLNKSDSPDFIKEQIHQ
                     TSIAQPALFITEYTLAKYLMQFGIIPKIMVGHSLGEYVAATLAGVFDLEDALNTVVER
                     GRLMQSTRHGSMLAVGLTQAEAEEYLSQDISLAAVNNPGLCVLSGAEDAIFNVQKRLD
                     GLNVFNKQLVTSHAFHSHFMDEILDSFYNHMSGIKLNKPSIPFFSCLTGRIADDKEVT
                     DPNYWVKQLRNTVKFCQCAQSFTENTEYVFIEIGPGNTLINHAKSCSKYNIVALQSLP
                     GAKENKSHSQILLECLGKMWLSGVDIKWEELYENERRRRIPLPLYPFERKTYICKTES
                     DRKHNSQLYVDKEWKALIYTSAWKQTILPASEVANDAGVHEWLIFTDSSREMNNCVAA
                     LIDRGHKVMIVKAGDSYKKLDQDIWTVNPENNDDMMQLAEDLKNTGISIDRCVYGWLM
                     EPSRSFSQENDKIYNTVCNTFIRAVDVLAMSGIMKDASEIYVVVSELFDVTAAKCFNP
                     LKRAIIGPCTTIPQEYPGMSCRVIDIPVEMLNRSSKILLTEILSGSIDTIVAYRGGKR
                     FIRSYEVISEDFIKRGGYDFEGGVFVIFNSAKEAGYQESFLKSQGNVKIEYRTGYNYD
                     EIRFALEDVHKKNGKINGVFYCLNISDELIKGVKQLTVDDLDCLSAKGLLHLKEAVQG
                     LGVEFCWVESSLSAIVGGVGFTAISAVANQLDALVESFCTDNWTENTKWICVNWDFMK
                     SSGKAHEKTSTWVVNRGISEEERNHIYERIFTLLGRVGNLYISSGRLASEVKRESMNK
                     NERTVINESSYQKRPKMANEYIEPRNEAERIVCEIIEELLSVKPVGIQDNFIDLGGHS
                     LLLTRVASRVKDVFRIDIPLKVVMDTPTVEGIMNYISTQWEESDIELIAMAYREYKKI
                     IDERE"
BASE COUNT         1058 a          869 c          516 g         1259 t
ORIGIN      
        1 attttccata acctttttcg cccaactcat aaaatgatca ctcatacttc tactcccttt
       61 catctataat ttttttatat tccctatatg ccattgcaat caactcaata tcgctctctt
      121 cccattgtgt cgatatgtaa ttcataattc cttcaacagt tggtgtatcc attactactt
      181 ttaagggaat atctatcctg aatacatctt taacacgtga agcaactctg gtaagaagca
      241 gtgaatgtcc cccaaggtca ataaaattat cttggattcc aacaggtttg acacttagaa
      301 gttcttctat aatttcacat actattcttt cagcttcatt acgtggttca atatactcat
      361 ttgccatttt aggccttttc tggtaactag actcattgat tacagttctc tcgtttttgt
      421 tcattgactc tcttttaact tcacttgcca gtctaccact tgaaatataa agattaccaa
      481 ctctgccaag taacgtaaaa atcctttcat aaatgtgatt tctttcttct tcgcttattc
      541 ccctgttaac aacccatgta gaagtcttct catgtgcttt accagaactc ttcataaagt
      601 cccagtttac gcatatccac tttgtgtttt ccgtccaatt atctgtacaa aaactctcta
      661 ccaaagcgtc aagctgattt gctacggctg atatagctgt aaaaccaaca cctccaacaa
      721 tagctgacaa agatgactct acccagcaaa actctactcc cagcccttga actgcttctt
      781 ttaaatgcaa caatcctttt gctgataaac aatccagatc atcaacagtt agttgtttta
      841 ccccttttat caactcatca ctaatattca ggcagtaaaa aactccattg atttttccgt
      901 tcttcttatg cacatcttcc agcgcaaacc ttatttcatc atagttataa cccgttctat
      961 attcaatctt cacattaccc tgcgatttaa gaaaagattc ctgataacct gcttcttttg
     1021 cagaattaaa tataacaaac actcctcctt cgaaatcata tcctccacgc ttaataaaat
     1081 cctccgaaat aacttcgtag cttcttataa acctttttcc tcctctataa gccacaatag
     1141 tatcaatgct tccagaaagt atttctgtca acagtatttt agatgatcta tttagcattt
     1201 caaccggtat atcaatcaca cggcaactca tccctggata ttcctgagga attgtagtac
     1261 atggcccaat tattgctctt tttaacggat taaagcattt agcagctgtt acatcaaata
     1321 attccgaaac tacaacatat atctctgaag catccttcat tattccagac attgccagta
     1381 catcaactgc acgtataaaa gtgttgcaaa cagtattata aattttatca ttctcctgag
     1441 aaaagctcct actcggttcc attaaccatc cataaacgca gcggtctata gatattccgg
     1501 tattttttaa atcttctgcc agttgcatca tatcatcatt gttttccgga ttcactgtcc
     1561 atatatcttg atccagcttt ttgtaagaat ccccagcttt cactatcata actttgtgac
     1621 ctctgtcaat caaagcagca acacaattgt tcatctccct gctactatcg gtaaatataa
     1681 gccattcatg cacaccagca tcatttgcta cttcagaggc aggtagtata gtttgcttcc
     1741 atgcgcttgt ataaatcaat gctttccact ctttatcaac atataattga gaattatgct
     1801 tcctatctga ttctgttttg caaatataag tttttctttc aaaaggatat aaaggaagtg
     1861 gtattcttct tcttctttca ttttcataca attcctccca tttaatatca acacccgaaa
     1921 gccacatttt cccgagacat tccaacagaa tttgactatg tgatttattt tcctttgctc
     1981 ctggaaggct ttgcagagct actatattgt actttgaaca gcttttggca tgattgatca
     2041 aagtatttcc cggaccaatc tctatgaaaa catactcagt attttcagta aaactctgag
     2101 cacactgaca aaacttcaca gtatttctca actgtttcac ccaataatta ggatcagtaa
     2161 cctctttatc atctgcaatt ctccctgtga ggcaggaaaa gaaaggtata cttggcttat
     2221 tcagcttgat gccgctcatg tggttataga aagaatccag tatttcatcc ataaaatgcg
     2281 agtgaaaggc atgagatgtt acaagttgtt tattaaaaac gttcaaacca tcaagtctct
     2341 tctgaacgtt aaatatagca tcctctgcac ctgatagaac acaaaggccc ggattattta
     2401 ctgccgcaag tgatatatcc tgtgacaagt actcctctgc ctccgcttga gtcaatccca
     2461 cagccagcat gcttccatgt cttgtacttt gcataagcct acctctctca acaacagtat
     2521 tcaatgcgtc ctccaagtca aatacacctg caagagtagc tgcaacatat tccccaagac
     2581 tatgtcccac cataatcttt ggtattatgc caaactgcat taaatatttt gccagagtat
     2641 actctgttat aaacaatgcc ggctgtgcta tgcttgtctg gtggatttgc tcctttatga
     2701 agtcagggct atcactttta ttaagcagat agttcctaat atcaaaacct agtttacatg
     2761 acactatttc cgccatcatg tctacctgct ctcttacaaa acaacttctc tcatataaat
     2821 cgcctatcat acctgcatac tgcgaacctt gtcctgtaaa cataaatgca acaggcctgc
     2881 acgaatactc ctgatatgta ctaaatactt tttgagcatc attgctttta agcgcctgtg
     2941 ctccatcttg agcatctgaa gcaatacata tcttcctgta gttatactgt cttcttccca
     3001 tttgaagagt aaaagcagca tcagccatgt taatctcatt ttctttttcg agaaagttcg
     3061 ccatgttatt agtcatttgc tctaaggaaa atgcactttt agcagacata atcaacaaaa
     3121 atctgtcaaa actatgtttt gcatctttct ccttttctgc atgttttgga gcttcttcca
     3181 gtatgagatg cacattagtt ccacctactc caaaagagct tattcctgct cttagtattg
     3241 cattttcctt tttccactca accggtgttg tattcacatt aaagcatttt tcaaaaaatt
     3301 taattttttg atttggctgt gtatagttta tttggggtgg tattttaccc ttgttcattg
     3361 aaagtacaac ttttataaag ctggcaacac ctgcagcggc atgaagatgc cctatattag
     3421 ctttaactga tccaatggtg cacctcggag tatcaattcc ctcaatacca aatgcttgtt
     3481 ttagagcttc tatttcaatg gggtcgccta cgttagtacc agtgccatga gtttcgacgt
     3541 agcttatact atgaggaggt attgaagaca tttcaaatac agtttcaata acttcttttt
     3601 gcccctcaat gcttggtgca ataaagccaa ccttccttcg tccgtcatta ttacacgcag
     3661 atgcttttat aactgccaat atagtatctc cgtcctcaag tg
//
LOCUS       DAONVY010000205         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17491_length_3682_cov_3.280673, whole genome shotgun sequence.
ACCESSION   DAONVY010000205 DAONVY010000000
VERSION     DAONVY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17491_length_3682_cov_3.280673"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLI11_06280"
     CDS             complement(<1..397)
                     /locus_tag="PLI11_06280"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPZ52505.1"
                     /translation="MRKLRVFLYIIKQGITGMVKNGFMIFASISVVFVALFILGSLNL
                     TSQNLQHIMDKLGDKPQIRIDLETETTDERVLEIEKALQEDPRVENVITVSKEEQYER
                     FLETFKDKKELFEGYSPELLFVSFEVKLKD"
     gene            complement(363..1133)
                     /gene="ftsE"
                     /locus_tag="PLI11_06285"
     CDS             complement(363..1133)
                     /gene="ftsE"
                     /locus_tag="PLI11_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPZ52506.1"
                     /translation="MIIFDNVVKRYPNGVYGLRGISFEIYEGEFVFLMGASGAGKSTI
                     IKLLMKEEDPTCGTIIANSQNLKKVKGRRIPRYRRDLGVVFQDFRLLEKKTVYGNVAF
                     AMEIMGHSPSEIKHRVRLALQLVGLSSKEKEYPSHLSGGEQQRVALARAIVNAPKIII
                     ADEPTGNLDPENSKEILEILNQINKRGTTVIVATHERDLALSMGKRIVYLDHGLQADD
                     RILCEEEMKVGSITGRKKRGLRKGGGSDEEAKSIFIHN"
     gene            complement(1184..2314)
                     /locus_tag="PLI11_06290"
     CDS             complement(1184..2314)
                     /locus_tag="PLI11_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPZ52507.1"
                     /translation="MNSSKDVRRQLDRIALIIGRDFGVIDEKDNKIYCSDVSDNAFNT
                     ITIPEELENDIDYSDFVQKDGYSFFRYAGSDSTVYLYMKSGENLDNDRRLLELAALAY
                     ENKEQIQKSEINHFYQSLLIDGPKSVNQSELTQYGIKGGYGFMVVNIYISSDDYISNS
                     ESSSITVLLKGIFSQFQTYQIVILNSQKYAVVFGINEEKVEYEQIIETSTTIRDTLAS
                     ELMVDAYVSVGSLVGKLTEINISYKDAETAREAGLVFGLDKECYVYDKMGIEKLIYGL
                     PIRNCVSFLKETLGVEFLKDKNAKELLYTVKTFLYNNQNVSESARALYIHRNTLVYRL
                     DKFNKLTNLDSTRFEEGMKIGIALMIMKYLEKKAPGELEFER"
     gene            complement(2456..3568)
                     /gene="ugpC"
                     /locus_tag="PLI11_06295"
     CDS             complement(2456..3568)
                     /gene="ugpC"
                     /locus_tag="PLI11_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPZ52508.1"
                     /translation="MASVVFKNIYKRYPGGVTAVNDFNLEVADKEFLVLVGPSGCGKS
                     TTLRMVAGLEEVTSGELLIDGKLVNDIAPKDRDIAMVFQNYALYPHMSVFENMAFGLK
                     LRKTPKGEIKKRVHEAARILEIEHLLDRSIKALSGGQKQRVALGRAIVREPKVFLMDE
                     PLSNLDAKLRAQMRTEIIKLHQKLQTTIIYVTHDQVEAMTMGSRIVVMKDGYIQQVET
                     PQRLYERPVNMFVAGFIGTPQMNFVNCRVEKINDDIFLRIGRWTIKLPEVKAKRLEEG
                     GYIDKEVVMGIRSEHVHDEAMYLEAYKDSQIESTVEVLELLGAESLVYMMLEGHVFIA
                     KIKPRHNIYVGDTMRFALDPNKIHVFDKDTENVITN"
BASE COUNT          984 a          860 c          579 g         1259 t
ORIGIN      
        1 aatccttaag ttttacctca aacgatacaa aaagaagttc aggactatag ccttcaaaaa
       61 gctccttttt gtccttgaag gtctccaaaa atctttcata ttgctcttcc ttgctaactg
      121 ttataacatt ctcaactctt ggatcttcct ggagtgcctt ctctatctcc agaactcttt
      181 catcagtagt ttcagtctcg aggtctattc taatctgagg cttgtctcct agcttatcca
      241 ttatatgttg gaggttttgg gatgtaagat tcagtgaacc cagaataaac agcgccacaa
      301 ataccactga tatcgatgca aatatcatga agccgttttt caccattccg gttattccct
      361 gtttaattat gtataaaaat actcttagct tcctcatcgc tgccacctcc ttttctcagt
      421 cctctttttt ttctgccggt tatgcttccg actttcattt cctcttcaca cagaattctg
      481 tcgtcagcct gcaggccatg gtcaagatac acaatccttt tgcccatgga aagagccaga
      541 tctcgttcat gggttgcaac gattacggtt gttccccttt tgtttatttg attcaatatc
      601 tccagtatct cttttgagtt ttccggatca aggttgcctg ttggctcgtc tgcaataatt
      661 attttgggag cattaacaat agctcttgca agcgccactc tctgctgctc tccaccagac
      721 agatgagaag gatattcctt ttccttgctg gatagtccga caagctgcaa agcaagcctc
      781 accctgtgtt ttatttcaga gggtgaatgt cccattatct ccatggcaaa ggcaacattg
      841 ccatagactg tctttttttc aagcagtctg aagtcctgaa aaaccacacc caaatcacgt
      901 ctgtacctgg gtatccttct accttttact tttttcaagt tttgactgtt tgcgataatc
      961 gttccgcagg ttggatcctc ttccttcatt aatagcttaa ttattgtaga tttacctgca
     1021 ccacttgctc ccattaaaaa gacaaactca ccttcatata tctcaaagga aatgcctcta
     1081 agtccgtata cgccgttagg atatcttttt accacattgt caaatataat tattgggcta
     1141 tccatatgta ccatcccttt ttcatttgta ttcaatttaa acttcatctt tcgaactcta
     1201 actcccccgg tgcctttttc tctaaatatt tcataatcat caatgctata ccaatcttca
     1261 ttccttcttc aaatctggta ctgtccaagt ttgtcagctt gttgaattta tctagcctgt
     1321 agacaagcgt atttctgtga atgtataaag ctcttgcaga ttcgcttaca ttctggttgt
     1381 tgtacaagaa ggtcttcact gtatacagaa gctcctttgc atttttgtct tttaaaaact
     1441 caacacccaa agtctccttc aggaacgata cacagtttct aatgggaaga ccataaatta
     1501 gcttttctat acccattttg tcatagacat agcattcttt gtccaaacca aaaacaagtc
     1561 ctgcttctct tgcagtttcc gcatctttat aggatatgtt tatttccgtc agcttgccaa
     1621 ccaaagaacc aactgaaaca tatgcgtcca ccataagctc cgaagcaagg gtatccctaa
     1681 tagttgtact tgtttcgatt atctgttcat actctacttt ttcttcattg attccaaaaa
     1741 ccacagcata cttctgggag ttcaggatta ctatctggta tgtctgaaat tgagaaaaga
     1801 taccctttag aagtacggta atggaggatg attcgctgtt gctgatataa tcatcgctac
     1861 ttatataaat attcacaacc ataaacccgt atccaccttt aattccgtac tgggtcagtt
     1921 cactttgatt tacagacttt ggaccatcta tgagcaaaga ctggtagaaa tgatttattt
     1981 cacttttttg aatctgctcc ttgttttcat atgcaagagc tgcaagctca agaagccttc
     2041 tatcattatc caggttctct ccggatttca tgtaaagata aacggtactg tcagaccccg
     2101 catatctgaa aaatgaatat ccatcttttt ggacaaaatc actgtagtcg atatcatttt
     2161 ctagttcttc aggaatagta attgtattga aggcattatc cgatacatca gagcaatata
     2221 ttttgttatc cttttcatca attactccga aatccctgcc tattattagt gcaattctat
     2281 caagttgtct tctgacatcc ttactactgt tcaaaaatcc actcttctcc cattcaagat
     2341 ttatacaatg ctattatact tgcttatact acccttttca aggtaaatat atggcactca
     2401 atgaaaaaaa gccccacaca aagtgaggcc tgatattcaa taatatatat tccaattagt
     2461 tggttataac gttctcggta tccttgtcaa atacatgtat tttgttagga tcaagtgcaa
     2521 atctcattgt atcgccaaca taaatattgt gccttggctt aatctttgca ataaagacat
     2581 gtccttctag catcatgtat accagggatt ctgcaccaag caactctaga acttctacag
     2641 tggactcaat ctgtgaatcc ttgtacgctt caagatacat cgcttcatca tgtacgtgct
     2701 cggaacggat acccattaca acttccttgt caatgtagcc gccttcttca agacgctttg
     2761 ccttaacttc tggaagtttt attgtccatc taccaattct gaggaaaatg tcatcgttaa
     2821 tcttctcaac acggcagttt acaaagttca tttgtggagt tcctataaat ccagcaacga
     2881 acatgtttac aggacgctcg tagagacgct gtggagtctc aacctgctga atgtaaccgt
     2941 ctttcataac aacgattctc gaacccatcg tcatagcctc tacctggtcg tgagtaacgt
     3001 agataattgt tgtctgcagc ttttgatgca atttaataat ctcggttctc atctgtgcac
     3061 gaagctttgc gtccaggttt gaaagcggtt cgtccattag gaacaccttt ggttcacgaa
     3121 cgatggcacg tccaagagca actctttgct tctgaccacc ggacaacgcc ttaatggatc
     3181 tgtcaagcaa atgctctatc tcaagaattc ttgctgcttc atgtactctc tttttaattt
     3241 ctccctttgg tgtctttctc aatttaagtc caaaagccat attctcaaat accgacatat
     3301 ggggatacag ggcatagttc tggaaaacca tcgctatatc tctgtccttt ggagctatgt
     3361 cattaaccaa tttaccatct atcaacagct ctcctgaggt aacctcttca agtcctgcca
     3421 ccattctcaa tgtagttgat tttccgcatc ctgatggtcc aaccaaaaca agaaactctt
     3481 tatctgccac ttccaggtta aagtcattta ccgctgtaac tcctccagga tatctcttgt
     3541 agatgttttt aaaaacaacg cttgccatta tgtaacctcc tatttattgt tcacatgaat
     3601 ttttttggtt ttaacgtatt cacttattgt attgcatacg taatttcatt cttccctatt
     3661 atatcacttg gctcatattt ta
//
LOCUS       DAONVY010000206         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17551_length_3673_cov_2.672747, whole genome shotgun sequence.
ACCESSION   DAONVY010000206 DAONVY010000000
VERSION     DAONVY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17551_length_3673_cov_2.672747"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..495)
                     /locus_tag="PLI11_06300"
     CDS             complement(121..495)
                     /locus_tag="PLI11_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52509.1"
                     /translation="MRGYNKWSFRPYIPYDRKSDKPYICRLEPGEDYICGEWFDLAYT
                     GEHTLYYSQLGKDDVKSLPLCNNHFTMENLKPDTEYELFIKGKERSNTRVARTDGVPG
                     KVVSYLHYMDTQYDFSGHSLCY"
     gene            complement(640..1605)
                     /locus_tag="PLI11_06305"
     CDS             complement(640..1605)
                     /locus_tag="PLI11_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPZ52510.1"
                     /translation="MRRQKSVFHETYHIVNRGFGGISIFRNDKDKSKYLELLAKGAEK
                     YSVDIIAYCLMDNHYHLCVSSRNEEYANISSFLRYANSQYVRYFNRTSEPDEDGDPRA
                     GSLFGTPYKAVPVNDEFQLEAVIAYIHNNPAPLGIDVKEYKYSSYHRYLNVASGRKTV
                     EEENEKRLVLKFFSVDKYTEEKLVELTRKSSKYKFCEGKESYIGKNYVTDELLIDQIE
                     YYYEMSISEINKLKNEEKLKIIYEISKMPGVSKKQIARIFSIDYHHLCRILRVYAQKI
                     SRQSSQTPAGLKNVSVKDTVNKSKHNCVTIIHSMMGQKSNSKRSP"
     gene            1911..2585
                     /locus_tag="PLI11_06310"
     CDS             1911..2585
                     /locus_tag="PLI11_06310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPZ52511.1"
                     /translation="MKNFLKSKAYYIVLLGCVVALCVGGIFVLKNKTDNNNPIIAKPT
                     ATPSPTVTKTPSSSTATEVPSGGDPVNNPKTEKPSPTPGSSPNVVKMTMPLDGEIKTG
                     YAADRLVYNKTLKEWRTHPAIDIVGEVGAEVKSAYDGTIADIKTDPHYGLTIIVDHGN
                     DLKTVYSGLAVNSEITLGSTVSAGDVIGKLGAGAFIERADGAHLHFEVIQNGKKVDPG
                     LFLDID"
     gene            2741..3577
                     /locus_tag="PLI11_06315"
     CDS             2741..3577
                     /locus_tag="PLI11_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPZ52512.1"
                     /translation="MERFTVKIATRYNSKWLFIKEEDRKFYRMPGGRAKHGEKIYSQV
                     KNLLYEQLGVVEACLYRVNEVYFYADVKCIGAVPLDNGIDDVAEVHENSVYDVSASSP
                     MRILSTAERNSFIKIHDWLFRRRHLFKEPCVQFEKVCGAVPFKEVDGIRYFLLVKNES
                     GHIGFPKGHVEEKETEEETAKREMFEETGLDIPLLDGFRYSFEYIIPKYKRRRKTIKL
                     AVYFIAEFQDETIKIQTSEISRWWLVPYKEALSILNKENDRELLRHANAWLDKTKKIS
                     KY"
BASE COUNT         1105 a          681 c          740 g         1147 t
ORIGIN      
        1 aatcagtgac ataataccag cttttcccgt cgtcgtcgga gcgaaaaaga gacgtcagat
       61 tctgtggact ctttgctttg taatagtcgt tggaagcaag cagcgaacca tttggtagct
      121 ttaatagcaa agactgtgtc ctgaaaaatc atactgggta tccatgtaat gaagataact
      181 tacaaccttt cctggtacac catctgtcct tgccactctt gtgttgcttc tttcctttcc
      241 ttttatgaat aattcatact ctgtatccgg tttcaagttt tccattgtaa aatggttgtt
      301 acaaagaggc agggatttta catcatcttt ccctagctga gaataatata gtgtgtgttc
      361 accggtgtat gccagatcaa accattcgcc gcatatgtaa tcttctcctg gttcaagacg
      421 gcagatatat ggtttgtcag acttcctatc gtacggtatg tatggtctaa aactccactt
      481 gttgtaacct cgcattgtat cacctggctt tgtataatat aagttctgcc aattgtccac
      541 agaaggtctt tcctgctgca agctatgcca ctaatctata ttatttttat acttatatca
      601 agacaaaagt tgcctctaac ttctttttgc atgcttaaat tatggtgatc tcttggaatt
      661 gcttttttgc cccatcatgc tgtgaatgat agttacacaa ttgtgcttgc tcttgttgac
      721 tgtgtccttc acagaaacat tctttagacc tgctggtgtc tgggaagatt gtcttgagat
      781 cttttgtgca taaactctta agattctgca caagtgatga taatcaatgg aaaatatcct
      841 tgctatttgc ttctttgata cgcctggcat tttgcttatc tcgtatatga ttttcagctt
      901 ctcctcgttt ttcaacttgt ttatttcact tatactcatt tcataataat actcaatttg
      961 gtcaattaat agctcgtcag tcacatagtt tttgcctatg taactctcct tgccttcgca
     1021 gaatttgtac ttgctgcttt tccttgtaag ctcaacaagc ttttcctcag tatatttatc
     1081 tactgaaaaa aacttaagaa caagtctttt ttcgttttcc tcttcaactg tttttcttcc
     1141 gcttgccaca ttaagatatc tgtgatagct gctgtatttg tattctttta catcgatacc
     1201 caagggagcg gggttgttgt gtatgtatgc aatcactgct tccaactgaa actcgtcgtt
     1261 tacaggcacg gccttgtagg gggttccgaa cagtgaaccc gctcttggat caccatcttc
     1321 atcaggctcg cttgttctgt tgaagtacct tacatattga gagtttgcat atcttagaaa
     1381 acttgatata tttgcatact cttcattcct tgagctgaca cacaggtggt agtggttgtc
     1441 cataaggcaa taggctatta tgtccacact gtacttttct gctccctttg caagcaactc
     1501 caggtatttg cttttgtctt tgtcatttct aaatatactt atgcctccaa aacctctgtt
     1561 tactatatga taagtttcgt gaaacacact tttttgacgt cgcaacttgc agcctcctga
     1621 atatttattg cttcgtgata ggtagtgtgc agtgtaaact gtatatgatg tctcgttttt
     1681 taagaagcgt aaacaggata tcatatttcc cggtatttcc ctgtcgaatt tcgacgaatt
     1741 caaaaatttt ttggaataaa tatttgggat aaataattgg tctgagcaaa gaagagaaga
     1801 gtaaagaaaa acttactgta aatctcttca aaatttattg cataattttc caccatagtt
     1861 gtataagtta ccaattctaa ggaaacaatt aactatggag gttgatatac atgaaaaatt
     1921 ttttgaaatc gaaagcatat tatatagtat tgctaggctg tgtagtggca ctttgtgtag
     1981 gaggaatatt tgtattaaag aataagacag ataataacaa tcctataatt gcaaaaccta
     2041 cagcaacacc ttcgcctaca gttacaaaga caccatcttc cagcactgct acagaagtac
     2101 cgagtggggg cgacccggta aacaatccaa agacagaaaa gccaagccca acacctggtt
     2161 cttcaccaaa tgtagtgaag atgacaatgc ctctggatgg tgaaatcaaa acaggctatg
     2221 cggctgacag gcttgtgtac aacaaaactt taaaagagtg gagaacgcat cctgcaattg
     2281 atatagtcgg tgaggttggt gcagaggtta aaagtgccta tgacggaaca attgccgata
     2341 ttaagacgga tccacattat ggtctgacca ttatagtaga ccacggcaat gatcttaaga
     2401 cagtttatag tggactggct gtaaacagtg aaattacgct tggcagtact gtctccgcag
     2461 gtgatgtcat aggaaagctc ggagcaggtg cattcatcga acgagcagac ggtgctcatt
     2521 tacactttga agtaattcag aatggcaaga aagttgatcc cggcttattc ctggacattg
     2581 actaattgac taatagtaat cgtatgtgtc tgcttttgaa aaaaggcaca tgggtttgca
     2641 gcccgtgtgt ctttttcatt tatagatact gtgcaatatc aaactttact cagtgcaaac
     2701 cgagtgatat aataaataaa aggggcgagg agaaatcaaa gtggaaagat ttacggttaa
     2761 gatagctacc agatacaaca gcaaatggtt gtttataaaa gaagaagata gaaagttcta
     2821 cagaatgcct ggtggaagag ctaaacatgg agagaaaata tacagtcagg tcaagaacct
     2881 gctgtatgag cagctcggtg ttgtggaagc ttgtttgtat cgtgtaaatg aagtttattt
     2941 ttatgccgac gtaaaatgca taggtgccgt accccttgac aatggtatag atgatgtagc
     3001 cgaagttcat gaaaatagtg tatatgacgt aagtgccagt tctccaatga ggattttgtc
     3061 aactgcagaa agaaactcgt ttattaaaat ccatgactgg ttgttcagaa gaagacattt
     3121 gttcaaggag ccttgtgtgc agtttgaaaa ggtttgtgga gccgttcctt ttaaagaagt
     3181 ggacggcata aggtattttc ttcttgtgaa aaatgagtca ggtcacatag gatttccaaa
     3241 aggtcatgtt gaagagaagg aaacggaaga agagacagca aagcgcgaga tgtttgaaga
     3301 aacaggcttg gacatacctc ttttggatgg attcagatac agctttgaat acataatacc
     3361 aaagtacaaa agaaggcgaa agacgataaa actcgccgtc tacttcatag cagagtttca
     3421 agacgaaacc atcaaaattc aaacaagtga aatctcaaga tggtggcttg ttccatacaa
     3481 ggaagctctg agcatactga ataaagagaa tgacagggaa cttttgaggc atgccaacgc
     3541 atggcttgac aaaactaaaa agataagcaa atattagtca tattttcacc tcttcacaag
     3601 tatatttcat taagtgatat atacaaatca agaggtgacc tcaatgacta aaccaagtag
     3661 aaagagaata att
//
LOCUS       DAONVY010000207         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17580_length_3668_cov_3.449211, whole genome shotgun sequence.
ACCESSION   DAONVY010000207 DAONVY010000000
VERSION     DAONVY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17580_length_3668_cov_3.449211"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..499
                     /locus_tag="PLI11_06320"
     CDS             83..499
                     /locus_tag="PLI11_06320"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPZ52513.1"
                     /translation="MRAISIKKRTVIILLSFAAAALLALLYFFEPVKGGIFPPCPFNY
                     LTGLYCPGCGTLRGIHSLLHGNLLKAMDYNILMVLSLPLVFYLYLMELGIKIKGKPLV
                     KTRPFSKTFYTITITVIVVYWILRNVNIFPLNILAP"
     gene            complement(512..2239)
                     /locus_tag="PLI11_06325"
     CDS             complement(512..2239)
                     /locus_tag="PLI11_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52514.1"
                     /translation="MKLLWRLGKEAIRYKGLYFIAIFSTFSLTIINLAAPKILTRMTG
                     IVESGESKDYFDVILKLTFILIALYLLRVLFRYLANFLAHKAAWQLVEDIRVRIYDHI
                     QGLSMSFFHDKQTGDLMSRVINDTVTFELLYAHIIPEMLTNAVTVIGVMVMLLAINYR
                     LALLTFIPIPFILIAGWLYAKKVRPNFRAAQKYQAEINAKLQDNFSGIHEIQAFCQQE
                     YESSQVRQKAKEYTKANLHALNLSGIFHPSVEFLSSTGTIIVVGIGGMFAHAWGLSVS
                     DIIAFLLYLSLFYAPISSLARVLEEMQQAYAGAERVMAVLDTPSEIKEAKNAVVLKDV
                     KGRITFEDVSFKYQTGGMVLKDISFEAKPGQMIALVGPTGVGKTTLTQLISRFYDPTE
                     GRVLIDGVDVKDVTLNSLRYHIAPVLQDTFLFNGTVAENIAYAKPDATMDEIINAAKA
                     ARIHNSIMEMPDGYQTRVGERGLRLSGGQKQRVAIARAILRNSPIIILDEATASVDVE
                     TEREIQKAIASLAGKRTIIAIAHRLSTIQNADLILVLEEGRIVQRGTHEELMQQDGLY
                     RRMSKSQSA"
     gene            complement(2338..3546)
                     /locus_tag="PLI11_06330"
     CDS             complement(2338..3546)
                     /locus_tag="PLI11_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52515.1"
                     /translation="MNRRIKIGVFGAWRGQSFISLLKHFKEEAYCCAVCDKNEQMLEH
                     VKQWCEEDTRFFTDVDDFFEFEMDAVILCNYFHEHAPYAVRALDKGIHVLSETTAAGT
                     LKECVELVEAQERSSAIYALAENYPYMLCNQELERIYKGGTLGKVIYAEGEYVHPMNI
                     NEAKYLTPNSLHWRAWLPRTYYLTHSLAPLMMITDTMPVYVNAKSVHSELFDEMLVKT
                     GIRKVSDMVSIMLVEMDNGALFRVTGSASFGPHGNWYRLACEKGGAESIRGNQNLVRL
                     AYNPWNTPEGEETERTYQPEWPSHKELAEKAGHGGGDFWVIYHFLNNIKEGTKPYFDV
                     YKAVSMSAVGILAWRSSLERGAEYKIPDFRNKAERDKCRNDDLSPFPDENGKATLPCS
                     SKYADWYKKV"
BASE COUNT          952 a          883 c          690 g         1143 t
ORIGIN      
        1 catactgtcc gcagttattg ctgctgtatt tactgtcttt tatttgatca ttattgtaat
       61 agcaagtgtg ggaatatgat aaatgagggc gatttccata aagaaaagaa ctgtaataat
      121 tcttttgtcc tttgctgcag ctgcattact ggctctacta tacttttttg aacctgtcaa
      181 aggaggaata tttccgccct gtcctttcaa ttacctgacc gggctttact gccctggatg
      241 cgggactttg agaggcattc actctttgct gcacggaaat cttcttaaag ccatggatta
      301 caacattcta atggtactat ctttgcctct ggtcttttat ctgtatctta tggagcttgg
      361 tataaagata aagggaaagc cgcttgtcaa aacgcggcct tttagcaaaa ccttttacac
      421 aatcaccata acagtaattg ttgtttattg gattttaaga aacgtaaaca tcttcccgtt
      481 aaacattttg gcaccttgac aggtgctttt tttatgcgct ttgggatttg gacattcttc
      541 tatacagacc atcctgctgc ataagttctt catgcgtacc tctctgtaca attcttcctt
      601 cttcgagaac aagtatcaag tccgcattct gtatagtaga cagtctgtgg gcaatggcaa
      661 ttattgttct ctttcctgca agggaagcaa ttgccttttg tatttctctt tccgtttcca
      721 catctactga tgcagtagct tcgtcaagta taatgatagg cgagtttcta agaattgctc
      781 ttgcaatggc cactctctgc ttctggccgc cgctcagtct aagccccctt tccccaaccc
      841 gggtctggta gccatcaggc atttccataa tgctgttatg tatcctcgct gctttcgccg
      901 catttataat ctcatccatg gtagcatcag gtttcgcata agcaatattc tccgccacag
      961 ttccattgaa caggaaagta tcctgcaata ctggtgcaat atgatacctg agactgttga
     1021 gagttacatc ctttacatca acgccgtcaa tcagcacacg accttcggta ggatcataga
     1081 atcttgatat tagctgcgtc aaggttgtct ttccaactcc agtaggaccc accagggcaa
     1141 tcatctgtcc aggctttgct tcaaaggata tgtctttcaa aaccattccg cctgtctggt
     1201 acttgaagga tacatcctca aaagtaattc tgccttttac atccttaagc acaactgcat
     1261 ttttggcttc cttgatttct gaaggggtat ccagaacagc cattactctt tcagcacctg
     1321 catacgcctg ctgcatttcc tctagcactc tggcaagtga tgatatgggt gcgtagaaca
     1381 gagacaaata gagcagaaac gcaattatgt ccgaaacgga caatccccat gcatgagcga
     1441 acattccgcc tatacctaca acaattatgg ttcctgttga tgacaggaac tcaactgatg
     1501 gatggaatat tccgcttaag ttaagagcgt gaaggtttgc tttagtgtat tcctttgctt
     1561 tctgtcttac ctggctggac tcatattcct gttggcagaa tgcttgtatt tcgtgtattc
     1621 ctgaaaagtt gtcctgcaac ttcgcattaa tttcagcctg gtatttctgt gctgccctaa
     1681 aattcggtct taccttcttt gcatacaacc aacctgcaat cagtataaaa ggaattggaa
     1741 taaaggttaa gagcgcaagt ctgtaattta tcgcaagcag cattaccatt acgccaatta
     1801 cggtcactgc atttgttagc atttcaggta tgatatgagc gtataatagc tcaaaggtca
     1861 cagtatcatt aataacccgg ctcatcaggt ctcctgtctg cttatcgtgg aaaaaactca
     1921 tggacagccc ctgtatatgg tcatatattc taacccttat atcctccacc agctgccagg
     1981 cagccttgtg cgccaaaaag tttgccaggt atctgaacaa gactctgagc agatacaaag
     2041 caatcagtat aaatgttagt tttagaatca catcaaaata gtccttgctt tccccgcttt
     2101 caactatgcc tgtcatcctt gtaagtatct ttggagcggc aaggttgatg atggtcagcg
     2161 aaaaggtaga aaaaatggca ataaaataaa ggcctttgta tcgtatagct tcttttccta
     2221 gtctccagag aagtttcatc cttagcctcc taaaattctg tgttcagtat attttatcaa
     2281 agtattgtga aaaaatcatc acagaataag aaacccggcc tctggccggg cttttaacta
     2341 caccttttta taccagtccg catattttga tgagcagggc agagttgctt ttccgttttc
     2401 atcaggaaaa ggcgacagat cgtcatttct gcacttatct ctctctgcct tgttcctaaa
     2461 atccggtatt ttatactccg ctcctctttc aagcgagctt ctccatgcca gaattcccac
     2521 tgcagacatg gaaacagcct tgtacacatc aaagtatggt ttggtgcctt ccttgatatt
     2581 attcagaaaa tgatagatca cccagaagtc tcctccaccg tgacctgcct tttcagcaag
     2641 ctccttgtgg gaaggccatt caggctgata tgtcctttct gtctcctctc cctctggtgt
     2701 gttccagggg ttgtacgcaa gccttacaag attttgattg ccccttatgg attctgcacc
     2761 acccttttca caggcaagtc gataccagtt gccatgcggg ccaaagctag cactgccggt
     2821 caccctgaac aaagcaccat tatccatttc aaccagcata atggaaacca tgtcacttac
     2881 ctttcttata cctgtcttta caagcatttc atcaaaaagc tccgagtgaa cactttttgc
     2941 attgacataa acaggcatgg tatccgttat catcatcaaa ggagcaaggg aatgggtaag
     3001 ataatatgtg cgcggcagcc atgccctcca atgcagtgaa tttggggtca ggtattttgc
     3061 ctcatttatg ttcatgggat gaacatattc gccttctgca taaatgactt tgcccagcgt
     3121 gccgcctttg tagattcttt caagctcctg gttgcacagc atgtatggat agttttctgc
     3181 aagagcataa attgcacttg aacgctcctg tgcttcaaca agctccacgc actccttcaa
     3241 agtgcctgca gcagtagttt cactaagtac atggattccc ttgtccaatg cccttactgc
     3301 atagggtgca tgttcatgga agtagttgca caagataaca gcatccattt caaattcaaa
     3361 aaaatcatcc acatcagtga aaaaccgggt gtcttcctca caccactgct ttacatgttc
     3421 cagcatctgt tcgttcttgt cgcatactgc acagcaatat gcctcttcct tgaaatgctt
     3481 gagaagactt atgaaagact gtcctctcca ggcaccgaaa acacctatct ttatcctcct
     3541 gttcatttgt atcctcctcg cattttttta cattatacat cacaagaccc aaaattcaag
     3601 tcggtaacta atatccttcc tccacaagtc ttggacattc ttccagtttc ctgtacgctt
     3661 ttatgtac
//
LOCUS       DAONVY010000208         3625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17823_length_3625_cov_3.470028, whole genome shotgun sequence.
ACCESSION   DAONVY010000208 DAONVY010000000
VERSION     DAONVY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17823_length_3625_cov_3.470028"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLI11_06335"
     CDS             complement(<1..686)
                     /locus_tag="PLI11_06335"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPZ52516.1"
                     /translation="MTLKWDEFVDKVYGCWIGKCVAGTIGAPYEGYKGIMNVEYTPSM
                     IDNMLPNDDLDLQVLWLDVIEKKGTNFTSLDLAEAFFNLCDYSPGEYATFRKNYDLGI
                     MPPLSGKFNNNYYIEGMGCPIRSEIWACLAPGDAKLATELAAMDGCLDHYGESIIAEQ
                     YLAALESLAFTDKWDIKPLVIRALNYIDEKSRVYELVSKVVDWCDQSDNWRYVFGRIL
                     RDYGHPDCTN"
     gene            907..2031
                     /locus_tag="PLI11_06340"
     CDS             907..2031
                     /locus_tag="PLI11_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ52517.1"
                     /translation="MTIRENVMAILNYEKFERMPIIAFGYWAETVDKWAEEGHISKED
                     AENYKRYGDNGPGDKAIMSKLGFDYAWNPQVAGHHFLYPAFETTVLEVEEDGSQIMRD
                     SAGLIVKVKPGVVSIPSEIGTTLTDREAWEEHYLPKLQYTDNRVNIEAIKAVRKNHPK
                     DLPFGIHCGSLMGNIRNMLGVLQLSYLYVDDEDLYTEIIDTFGDLCYKVAKRIVETGI
                     EIDYAHFWEDICYKNGPLVTPSVFEEKVGPHYKRITDLLKKHNINIVSLDCDGCIDSL
                     VPIWVENGVNTMFPIEVGTWKADIRPWREKYGKELRGIGGMNKNVFAMDKKAVEEEVE
                     RLKALVSLGGYIPCPDHRIPPTADFSLVAYYCELMAKTTF"
     gene            2112..3326
                     /locus_tag="PLI11_06345"
     CDS             2112..3326
                     /locus_tag="PLI11_06345"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPZ52518.1"
                     /translation="MAKEKVVLAYSGGLDTSIIIPWLKENYDYEVIAVAADVGQGEEL
                     EPLREKAIRTGASKIYIEDLKHDFVTNYIFPTLKAGAIYEGDYLLGTSFARPCIAKRL
                     VEIAEQEGAVAIAHGCTGKGNDQVRFELTIKALAPHMKIIAPWRIWDIKSRDEEIDYA
                     LERGIDVPVTKENNYSMDRNIWHLSHEGMDLEDPWNEPKYDKILQLIVPPEKAPDEPS
                     YVEIEFEKGVPGKVDGKTMDPVELLTYLNKVAGENGVGIVDMVENRLVGMKSRGVYET
                     PGGTLLYKAHRALELLCLDRDTLHYKDILAQRYAELVYYGQWFTPLKEALDAFFDSTQ
                     KTVSGTVKLKLYKGNCMLAGTKSPYSLYDEDIATFGADDVYNQKDSEGFINLFGLPVK
                     INALMKKRNELK"
     gene            3323..>3625
                     /locus_tag="PLI11_06350"
     CDS             3323..>3625
                     /locus_tag="PLI11_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPZ52519.1"
                     /translation="MKLWGGRFSEATDKIVDDFNSSIRFDQRLYYYDIKGSIAHARML
                     GNCSIIPKEEAELIIAELNAILKDIEDGKIEFEVDAEDIHMNIEKILIDRIGDVGKK"
BASE COUNT         1188 a          717 c          787 g          933 t
ORIGIN      
        1 atgtttgtgc agtcaggatg accgtaatcg cggagaattc ttccaaatac atatctccag
       61 ttgtcggact gatcacacca gtcaacgacc ttgcttacaa gctcgtacac tcttgatttt
      121 tcatctatgt agttaagagc tctaataacc aaaggcttga tatcccattt gtcagtaaac
      181 gcaagagatt caagagcggc cagatactgc tcggcaatga tagactcacc gtagtgatca
      241 agacatccgt ccatagccgc cagctctgta gcaagtttcg catctcccgg tgcaaggcat
      301 gcccagattt cagagcggat aggacagccc attccttcaa tatagtagtt gttattaaac
      361 ttacctgaaa gaggaggcat tatgccaaga tcgtagtttt ttctaaaagt cgcatactct
      421 ccaggcgaat agtcgcacag attaaagaaa gcttctgcca aatccaaaga tgtgaagttt
      481 gtgcctttct tttcaataac atcaagccac aacacctgca aatcgaggtc gtcatttgga
      541 agcatgttgt caatcattga aggcgtatac tccacattca ttattccctt gtaaccttca
      601 taaggtgctc cgattgttcc agcaacacat ttgcctatcc aacaaccata taccttgtct
      661 acaaactcat cccattttag tgtcattttt aaatcctcca ctaccacatt ataattttat
      721 gcatatattc tatacatttt tcagtagtat atcaactttt ctttatattt acaacgattt
      781 gttttacttt tgttccttaa tttgtgataa aatacaacag aaacaacatt taatccacat
      841 gttgattatc ttgtgttgat aatcaaaaat aaataataca ttggaataaa ggagaggaca
      901 tgcaaaatga caatcagaga aaatgtaatg gcaatactga attatgaaaa atttgagaga
      961 atgccaatta ttgcgttcgg ctactgggcg gaaaccgttg acaaatgggc tgaagaaggc
     1021 catatttcca aagaagatgc tgaaaactac aaaagatatg gcgacaacgg acctggagac
     1081 aaggcgataa tgagcaagct gggatttgac tatgcctgga atccccaagt tgcaggccac
     1141 cactttttat accctgcatt cgaaactacc gttctcgaag tagaagaaga cggcagccaa
     1201 atcatgagag attctgcagg cctcatagta aaagtaaaac cgggtgttgt ctcaataccc
     1261 tctgaaattg gcacaaccct tacggacaga gaggcctggg aagagcatta cttgccaaaa
     1321 ctgcagtata cggacaacag agtcaacatc gaagctataa aagcagttcg caaaaaccat
     1381 ccaaaggatc ttccctttgg aatccactgc ggctcactta tgggaaacat aaggaacatg
     1441 cttggagttt tgcagctcag ttacctctat gtggatgatg aagacctata tactgaaata
     1501 attgatacgt ttggagacct ctgctacaaa gttgcaaaga gaattgttga aactggcatt
     1561 gaaattgact atgcacactt ctgggaggac atatgctaca agaacggtcc gttggtaact
     1621 ccttcagtat ttgaagagaa agtcgggcct cattacaaga gaatcaccga ccttttgaaa
     1681 aagcataata taaacatagt ttcattggac tgcgacggat gtatagattc acttgttcca
     1741 atctgggttg aaaatggagt caacaccatg ttcccaattg aagttggaac ctggaaggca
     1801 gacataaggc cttggagaga aaaatacggc aaggaactta gaggcatcgg tggaatgaac
     1861 aagaacgtat ttgcaatgga taagaaggca gtggaagaag aagtcgaaag actaaaagca
     1921 cttgtttccc ttggaggata cataccttgc ccggatcaca gaattccacc tactgcggac
     1981 ttctcactag ttgcatatta ctgcgagctg atggcaaaaa caaccttcta attgaaatgc
     2041 caaaaaaaaa tgtgtataat gttctgaacc aatattataa atattggcag aactgttcag
     2101 gaggaatgca tatggctaag gaaaaagttg tactagcgta ttcaggtgga cttgatacat
     2161 caattatcat tccatggctg aaagaaaact acgattatga agtaatagct gttgccgctg
     2221 atgtgggtca gggcgaagaa cttgaaccac ttagggaaaa ggcgattaga acaggggctt
     2281 caaaaatata tattgaagat ttgaaacatg attttgtaac caattacatc ttccctaccc
     2341 tgaaagcagg cgcaatctat gagggagact atctccttgg tacttccttt gcaagaccat
     2401 gtatcgcaaa gaggcttgtt gagattgcag aacaggaagg tgcggttgca attgcccatg
     2461 gctgcacagg caagggcaat gaccaggtaa gatttgagct taccataaaa gctcttgctc
     2521 cgcatatgaa aattatagcg ccatggcgta tctgggacat aaagtcgaga gacgaagaaa
     2581 ttgactacgc actggaaaga ggaattgatg tacctgttac taaggaaaac aactattcca
     2641 tggacagaaa catatggcat ttgagccatg aaggcatgga cttggaagat ccatggaatg
     2701 agccaaagta cgacaagatt ctacaactga ttgttccgcc ggaaaaggca ccggacgagc
     2761 catcttatgt ggaaattgag tttgaaaaag gagttccggg aaaggttgac ggcaagacta
     2821 tggacccggt tgaacttcta acctatctca acaaagtggc aggagaaaac ggcgttggca
     2881 tagtggatat ggttgaaaac agacttgttg gaatgaagtc aagaggagta tacgaaactc
     2941 ctggaggaac cctcctttac aaagcccaca gggcccttga acttttatgc ctagataggg
     3001 ataccctcca ctacaaagac attctggcgc aaagatatgc agaactcgta tactacggcc
     3061 agtggttcac cccccttaaa gaagctttgg atgctttttt tgattcaact cagaaaacag
     3121 tttcaggaac tgtcaaactc aagctctaca aaggcaactg catgctggca ggcacaaaat
     3181 ccccatactc actttatgac gaggacattg ccacctttgg agcagatgac gtatacaacc
     3241 aaaaagattc ggaaggcttc ataaatctct ttggtcttcc tgtgaaaatt aatgctttga
     3301 tgaagaaaag gaatgagttg aaatgaaact gtggggagga cgcttttctg aagcaacgga
     3361 taaaatagtt gatgacttca actcatcaat ccggtttgat caaaggctct actattacga
     3421 tataaaagga agtattgccc atgcaagaat gctgggcaat tgttctatta ttcccaagga
     3481 agaagccgaa ttgatcatag cggaattgaa tgccatactg aaggatattg aagatggcaa
     3541 gatagagttt gaagtggacg cagaagatat tcatatgaat attgagaaaa tccttataga
     3601 ccggattggt gacgtgggaa agaaa
//
LOCUS       DAONVY010000209         3604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17936_length_3604_cov_2.712032, whole genome shotgun sequence.
ACCESSION   DAONVY010000209 DAONVY010000000
VERSION     DAONVY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17936_length_3604_cov_2.712032"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLI11_06355"
     CDS             <1..339
                     /locus_tag="PLI11_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPZ52520.1"
                     /translation="VVDGRLCAILNQRSQDMLAANAWNVAQYAALVLMLAQVSNLKPG
                     ELVHVIADAHIYDRHIPIIEDLLAREEYESPTVLLDESIDDFYKFTPESFTVLNYMHG
                     EKIGRFPVAI"
     gene            355..876
                     /locus_tag="PLI11_06360"
     CDS             355..876
                     /locus_tag="PLI11_06360"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPZ52521.1"
                     /translation="MNLIVAVDTDWGIGYKGDLLARVREDLRNFAKVTSGKIVVYGTN
                     TLATFPGGKVLKNRINIILNPSPDFKVEGAIVANSLDDLFKKLESYNKEDIFVIGGAS
                     VYNQLLPYCTKAYVTKFDKSFDKDTYIPNLDESDDWICVYESEEFRTNPETDTGGDFS
                     FKFTIYEKTGKTK"
     gene            873..2015
                     /locus_tag="PLI11_06365"
     CDS             873..2015
                     /locus_tag="PLI11_06365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPZ52522.1"
                     /translation="MKVCHVCLCGPVTDNFSYQENLLTKYQAKLGMDVSMIASPYYFT
                     QEGTIAISDKKEYVNSDGVKLYRLKVKKGDYEDKFRRYDGLYETIERINPDVLFVHGV
                     QFLDLTEVVKYKKRNDYVKMYVDNHADFSNSATNFLSRYILHGVIWKRYAKLAYKHAE
                     KIYGVTPGRVRFLKEVYKLKDDKLGLLLMGADTDKVEEALNSNARKEIRTKYGIGEDD
                     FLIMTGGKIDYAKKQTLLLMDAVSTLDNAKLIVFGSVEEDLKEEVLKRAHGNVQYIGW
                     INAFDSYKYFSSADLVVFPGRHSVFWEQVLGLGIPMIVKYWEGNEHVDMGGNCLFLYE
                     DNVEEIREKISLVLKDKEFYENMKIIAKEKGAKEFSYLEIAKKSVE"
     gene            2045..3451
                     /locus_tag="PLI11_06370"
     CDS             2045..3451
                     /locus_tag="PLI11_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPZ52523.1"
                     /translation="MKKYDLVVIGSGAGLMVAEVAMQIGVKCALIEKDKFGGTCLTKG
                     CIPSKMLVYPADLIRESERADRVGVKLSKPRIDWEKIQERMWKQINYTYRIEDNLDKT
                     PDIDVYKGLAEFTGPNTLLVRYENGNTEEIHGDRIVVSAGARSFVPDIDGLEETGYVT
                     YETFFGKKFPKKLWKSVTILGGGIISAEFAHILSAFGVDVTIIARRGLLRHEEPEIRD
                     FVKRQFEKHGIKILANMDLMSVEKTKKGKSVTFKDRESGEISLVESEELFIAMGLRSN
                     SDILKVEKAGIEVDEKGWIVTNDYLETNVSHIYAIGDINGKYQFRHKANHEAEVLITN
                     LFGREKRKANYNAVPWTIFTHPQVSHVGLTEAEVKEKGIEYVTAFNYYSEVVGGISRG
                     YSDKSDDNGFVKVIVSKDRKILGVHAVGPHSSMLVQPFVYVMNSNDGTYDVVSESMVI
                     HPSLNELSAWVFEKIDLW"
BASE COUNT         1128 a          555 c          920 g         1001 t
ORIGIN      
        1 gtggtggatg gaagactttg tgccatactt aaccagcgct cccaggatat gctggcggcc
       61 aatgcatgga atgtagccca gtatgcagca ctggttctta tgcttgcaca ggtttccaac
      121 ctaaagccgg gagaactggt acatgtaatt gctgatgctc acatttatga tcgccatata
      181 ccgataatag aggatcttct tgcaagagag gagtatgaat caccgacggt tttactggat
      241 gaatccatag acgatttcta taaattcacg ccggagagct ttacggtatt gaactacatg
      301 catggtgaga agataggcag gtttcctgtt gctatttaat gaaaggacag ttttatgaat
      361 ctgattgtgg cagtagacac ggactggggt ataggataca agggagattt gcttgccagg
      421 gttagggaag acctcagaaa ctttgcaaag gttacttctg gcaagattgt tgtgtatggc
      481 actaatacct tggcgacctt tccaggcggc aaggtattga aaaacaggat taacataatt
      541 ttaaatccaa gccctgattt caaggtcgag ggtgcaattg ttgcaaactc tcttgatgac
      601 ttgtttaaga agcttgaatc ctacaacaaa gaggatatct ttgtcatagg cggggcaagt
      661 gtgtacaacc agctccttcc ttattgtacg aaagcatatg ttaccaaatt tgacaagtcg
      721 tttgacaagg acacgtatat tccaaatctg gacgaatctg atgattggat atgcgtttat
      781 gaaagtgaag agtttagaac aaatcccgag acagatacgg gaggagattt ttctttcaaa
      841 tttactatat atgaaaagac aggtaaaacc aaatgaaagt gtgccatgtt tgtttgtgcg
      901 gaccggtaac ggataatttc agctatcagg aaaatctgct gacgaaatat caggcgaagc
      961 tgggcatgga tgtttccatg atagcttcgc cctattattt cacccaggaa ggaaccattg
     1021 caatatcaga caagaaagag tatgtaaact ccgatggtgt gaaattatat cgtttgaaag
     1081 ttaaaaaggg agattacgag gacaagttta gaaggtatga tggcttgtat gaaacaattg
     1141 aaaggataaa cccggatgta ttgtttgttc atggtgttca atttctggat ttgacggagg
     1201 ttgtcaagta caagaaaagg aatgactatg taaagatgta tgtggacaac catgctgact
     1261 tttcaaacag tgccacgaac tttctgtcca gatacattct tcacggagtt atttggaagc
     1321 ggtatgcaaa gcttgcatac aagcatgctg aaaagattta tggggtaaca cccggaagag
     1381 ttagattttt gaaagaagta tacaagctta aggacgacaa gcttggtctt ttactcatgg
     1441 gtgcggatac agacaaggtg gaagaagctt taaacagcaa tgcccgaaaa gagataagga
     1501 ccaaatatgg cattggtgaa gatgattttt taataatgac tggtggaaag atagattatg
     1561 caaagaagca gactcttctt ttgatggatg cagtatctac tcttgataat gcaaaactta
     1621 ttgtttttgg ctcggtggag gaagatttaa aggaagaagt attaaaaaga gcacatggta
     1681 atgtccagta tattgggtgg ataaatgcat tcgactcata taaatacttt tcttcggcgg
     1741 atttggttgt ttttccggga agacactcgg ttttctggga acaggtactg ggtcttggaa
     1801 tacctatgat agtaaagtac tgggaaggca acgagcatgt ggatatggga ggaaactgcc
     1861 tttttctcta cgaagacaac gtggaggaaa taagggagaa gataagtttg gttttgaaag
     1921 acaaggaatt ttatgagaat atgaagataa ttgcaaagga aaaaggcgcg aaagagtttt
     1981 cataccttga gatagctaaa aagagcgtgg aataaaattt ctagtaatgt gggaggggat
     2041 gtaattgaag aagtacgatt tggtggttat tggtagtggg gcaggactga tggtagcaga
     2101 agttgcaatg cagataggtg tgaaatgtgc attgattgaa aaggataagt tcggtgggac
     2161 atgccttaca aagggttgca taccttccaa gatgctggtt tatcctgcgg atttaataag
     2221 ggaatcggaa agagctgaca gggttggagt caaactgtca aaaccccgca tagattggga
     2281 aaagatacaa gagagaatgt ggaagcagat taactacacc tacaggatag aggataatct
     2341 tgacaagacc cctgatatcg atgtttataa aggtttggca gagtttacag gaccaaatac
     2401 cttgcttgta agatacgaga atggcaatac cgaggagatt catggtgata gaatagttgt
     2461 cagtgcaggt gcaaggtcgt ttgttcctga tattgatgga ttggaagaga caggttatgt
     2521 tacctatgag actttctttg ggaaaaagtt tccaaagaag ctgtggaaaa gtgttaccat
     2581 cctgggtgga ggtataatca gtgccgagtt tgcccatatt ctgtccgcat ttggcgtgga
     2641 tgtcacaatt attgcaagaa gaggtttatt aagacatgag gaacctgaga taagggattt
     2701 tgtaaaaaga cagtttgaaa agcatggtat taaaattctt gctaacatgg acttgatgtc
     2761 agtagagaag acgaaaaaag gcaagagtgt gactttcaag gatagggaaa gtggcgagat
     2821 aagccttgtt gaaagtgagg agcttttcat cgcgatgggg cttcggtcaa attccgatat
     2881 attaaaggtg gaaaaggccg gtattgaggt ggatgaaaag ggctggatag tgactaatga
     2941 ttatcttgaa accaatgtgt ctcatattta tgccataggt gatatcaacg gcaagtatca
     3001 gttcaggcac aaggccaatc atgaagctga agttttaatt acaaacttgt ttggaaggga
     3061 aaaacggaag gcaaactata atgctgttcc ctggactatt tttactcatc cccaggtgtc
     3121 ccatgtagga cttactgagg cggaagtcaa agagaagggt attgagtatg ttaccgcatt
     3181 caactactat tccgaagtag tgggaggcat ctcaagagga tattcggata aatctgacga
     3241 caatggtttt gtaaaagtga tagtctccaa ggacagaaaa attcttggag tacacgctgt
     3301 agggcctcat tcatccatgc tggttcagcc ctttgtatat gtcatgaact caaatgacgg
     3361 cacatatgat gtggttagcg agtccatggt aatacatcct tcactgaatg agctttcggc
     3421 gtgggtgttt gaaaaaatag atttgtggta agaaaaaagg catctcgtgg atgccttttt
     3481 tgctatctgt attctggaag ccaacccttg tgagcttcaa ttaaatcatc acacattgct
     3541 acaatattat ctattgtgag ttcgctcgat gtatgtggat caagcatggc tgcatgataa
     3601 atat
//
LOCUS       DAONVY010000210         3601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17955_length_3601_cov_3.244783, whole genome shotgun sequence.
ACCESSION   DAONVY010000210 DAONVY010000000
VERSION     DAONVY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17955_length_3601_cov_3.244783"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..486
                     /locus_tag="PLI11_06375"
     CDS             52..486
                     /locus_tag="PLI11_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HPZ52524.1"
                     /translation="MVGIMEDKKYNIKDAKMLPPLVLAYIGDSVFELFIREKLVSSGI
                     RNVHKLHVNAITYVRAKSQSFMVHELADILNPDEQEIVRRGRNANTHTHPKNADISDY
                     RYATGFESLIGYLYLTNQTERMSHILERSFELMNKEKGFNEH"
     gene            476..1216
                     /gene="rlmB"
                     /locus_tag="PLI11_06380"
     CDS             476..1216
                     /gene="rlmB"
                     /locus_tag="PLI11_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPZ52525.1"
                     /translation="MSIDNEFIYGRNAVIEALKSDTPINKIMLVNEHGGSIGVIKALA
                     REKGIIFSITTKEKLTAITGTSSHQGVMAYLSARQYDTLEDILEHARELGQDPFVIVL
                     DEIQDPHNLGAIIRSADAAGAHGIVISKRRQVPLTAAVAKTSAGAIEHMKIARVSNVA
                     QTLDKLKKEGVWIVGTDADGDYSLFNCDLKGPVAVVVGSEGKGIGSLVKKKCDLVLSI
                     PMVGKITSLNASVAASLVMYEVLRQRSL"
     gene            1216..3447
                     /locus_tag="PLI11_06385"
     CDS             1216..3447
                     /locus_tag="PLI11_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPZ52526.1"
                     /translation="MDLKKELNKEQYLAATHVEGPLLILAGAGSGKTRVITYRIAHLI
                     RNCGVSPYNILAITFTNKAAREMAERASFLLGEENLYGIWINTFHAACGKILRRYADR
                     IGFNSNFIIYDETEVQVVLKECLRRLNIDEKYLDPKAVRKYISKAKDEMISWKDFTKS
                     HDTYNNHLFRMVSEVYKMYQETLLANNAMDFDDMILMTIKLFKENPDVLDYYQEKFKF
                     IMVDEYQDTNMAQYEFVRLLASKYKNLCVVGDDDQSIYGFRGADIRNILEFENEYHDC
                     KVIKLEQNYRSTQNILDAANRIIKNNTERRHKRLWTEQGHGEPITRYEAEDQNDEALY
                     IVSAIQDGVRKGMKYSDFTILYRINALSQTIEKALTRFGIPYRVYGGLKFFDRKEIKD
                     IVCYLRVLDNPLDDVALRRIINVPKRGIGEVSYNNAYDIALREDTNVLNVFLNAGNYP
                     ELSRTASKLASFALKIMDIMMKKDTMAISEYVKYVIYESGMIEELKRENTDEAESRIE
                     NLMEFISVANEYESEVLESPDAENLNNLTSFLQNIALSTDMDKQEEVDADTVTLMTIH
                     NSKGLEFPVVFVIAMEEGTFPSMRAEMPEEVEEERRLCYVAITRAKKKLYLTHSRLRL
                     LYGRTNYRVPSRFLRELELKAEKEEDRSEKSTTFRTGAVRKGALGRVVNSVGDVKALN
                     QQTGKVDTKDFKVGDRVKHKKFGNGTIVKMEGSGDKTVVEIQFDSTGMKRLMLAYAKL
                     SHI"
BASE COUNT         1183 a          591 c          899 g          928 t
ORIGIN      
        1 cagaacagta tctttgaagg attcctggtc gaaaaaggcg aagtccaatt aatggtagga
       61 attatggaag acaaaaagta caacataaaa gatgcgaaga tgcttccccc tcttgtactg
      121 gcatatattg gcgattcggt ctttgaattg tttattagag agaaacttgt ttcatcagga
      181 atcaggaatg ttcataaatt gcatgtgaac gccataacat atgtaagggc aaaatcccag
      241 tccttcatgg ttcatgagct tgcggacatt ctcaatcctg atgaacagga gattgtgaga
      301 aggggtagaa atgcaaatac ccatacgcat cccaagaatg cggacatatc agattacagg
      361 tatgcaacgg gatttgagag tctgattgga tacctttatc ttaccaatca gacggaacgc
      421 atgagtcata ttttggagcg ttcttttgaa ttaatgaaca aggagaaagg ctttaatgag
      481 cattgataat gaatttatat acggtaggaa tgctgtgatt gaggcattga aaagtgatac
      541 gcccattaac aagattatgc ttgttaatga gcatggtggg tccataggag ttatcaaggc
      601 cctggcaaga gagaaaggaa ttatattcag catcacaaca aaggaaaaac tgactgcaat
      661 tacaggtact agcagccatc agggtgtcat ggcatatttg tccgccaggc agtacgacac
      721 gctggaagat atattggagc atgcaaggga acttggtcag gatccttttg tcattgttct
      781 tgatgaaata caggatcctc acaaccttgg tgcaattatt agaagtgctg atgcggcggg
      841 agcccatggg atagtaatct caaagagaag acaggttccg ttgactgctg ctgtggcgaa
      901 aacctctgcg ggtgcaattg aacatatgaa aatagcgcgc gtgtcaaatg ttgcccagac
      961 tcttgacaag ttgaagaagg aaggagtttg gattgttggt acggatgcgg atggagatta
     1021 tagccttttc aactgtgact tgaaaggacc tgtcgctgtg gttgttggaa gcgaaggcaa
     1081 gggtataggt agccttgtca agaagaaatg cgatcttgta ttatcaattc caatggtcgg
     1141 aaaaataacg tcattgaatg catctgttgc agccagtttg gttatgtacg aagttttaag
     1201 gcaaaggagc ttgtaatgga tctgaagaag gaacttaata aagaacaata tttggcagcc
     1261 actcatgtgg aaggccctct actaattctt gcaggagcag gtagtggtaa aaccagggtt
     1321 attacctaca ggatagccca tttgataagg aactgcggcg taagtccgta caatatcctg
     1381 gcaattactt ttaccaacaa agctgcaaga gaaatggctg agagggcttc ttttttattg
     1441 ggcgaagaaa atctttacgg catatggata aacacattcc acgcagcctg tggtaaaata
     1501 ttgagaaggt atgcggatag aataggtttc aattcgaact tcatcatata cgatgaaacc
     1561 gaagtgcagg tggttttgaa ggaatgtctt agaagactta atatagatga gaaatacctg
     1621 gaccccaaag cggtaagaaa atacatatca aaagcaaaag acgagatgat atcttggaag
     1681 gattttacca agagtcatga cacatacaat aaccacttgt ttaggatggt ttcggaagtg
     1741 tacaaaatgt atcaggaaac cttgcttgcg aacaacgcca tggattttga cgacatgata
     1801 cttatgacaa taaagttgtt caaagagaat cctgatgttc ttgattatta ccaggagaag
     1861 ttcaagttca taatggtgga tgagtatcag gatacgaaca tggctcagta tgagtttgtc
     1921 cgtttgcttg catcaaagta caagaatctg tgtgtggtag gagatgacga ccagagcatc
     1981 tatggtttca gaggtgcgga tataaggaac atacttgagt ttgaaaacga gtatcatgac
     2041 tgcaaggtaa taaagctgga gcagaactat agatctacgc agaacatact ggatgcggca
     2101 aacaggataa ttaaaaacaa tacagaaaga agacacaaga gattgtggac ggagcaggga
     2161 catggagaac ccataaccag atacgaagcg gaagatcaaa acgacgaagc cttgtatata
     2221 gtaagtgcta ttcaggatgg tgttagaaaa ggaatgaaat attcggattt caccattctt
     2281 tacagaataa atgccctgtc gcagacaata gagaaagctc ttaccagatt tggaataccg
     2341 tacagggtgt acggcggttt gaagttcttt gacaggaagg aaatcaagga tatcgtctgc
     2401 taccttaggg tgttggacaa tcctttggac gatgtggcat taagaaggat tatcaatgtt
     2461 ccgaaaaggg gaattggtga ggtttcatac aacaacgcat acgacattgc actcagggaa
     2521 gacacaaatg tcttaaatgt gtttcttaat gcgggcaact atcccgagtt gtccagaacc
     2581 gcatccaagc ttgcaagctt tgcgctaaag ataatggaca ttatgatgaa aaaggataca
     2641 atggccattt ccgagtatgt aaagtatgtg atatacgagt cgggcatgat tgaagaacta
     2701 aagagagaga atactgatga agcggaatcc cgaatagaaa accttatgga gttcatttct
     2761 gttgcaaatg agtatgaaag cgaagtcctg gaatctcccg atgctgagaa cttaaacaat
     2821 ctgacttcat ttttgcagaa catcgccctc agtacagaca tggacaagca ggaggaagta
     2881 gatgcagaca cggtcactct aatgacaata cacaactcaa agggtcttga atttcctgtt
     2941 gtgttcgtaa ttgccatgga agagggtacc tttccgtcca tgcgcgcaga gatgcctgaa
     3001 gaagttgaag aggaaagaag gctttgttat gttgcaataa caagagcaaa gaaaaaactt
     3061 tatctgactc attcgaggct aagattgctc tacggaagaa ccaattacag ggttccttcc
     3121 agatttttaa gggagcttga gttaaaagct gagaaggagg aagataggag tgagaaaagt
     3181 acaactttca ggactggtgc tgtcaggaag ggagctttag gacgcgttgt caatagtgtt
     3241 ggtgatgtaa aagctctgaa ccaacaaacc gggaaggttg ataccaaaga tttcaaggtg
     3301 ggtgacagag taaaacacaa gaagtttggc aatggtacca ttgtgaaaat ggaaggcagt
     3361 ggtgataaaa ccgttgttga gatacagttt gacagtactg gaatgaaacg tcttatgctg
     3421 gcttatgcaa agctttcaca tatatagtgc ttgacatgat aggggtttta atgtaaacta
     3481 tgttcacttt attgctttat tgcgataaag tgaaggaggt ttgagatgaa acagatctac
     3541 acacctgaag gcatgcagga tattttgatt gaagaaggct tcaacatgag aaatatggaa
     3601 g
//
LOCUS       DAONVY010000211         3595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_17999_length_3595_cov_2.873446, whole genome shotgun sequence.
ACCESSION   DAONVY010000211 DAONVY010000000
VERSION     DAONVY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17999_length_3595_cov_2.873446"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLI11_06390"
     CDS             <1..483
                     /locus_tag="PLI11_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52527.1"
                     /translation="DMVDNVILKDYKRQKGLLLSRKPARKTALKMVEDLDIVTQGVQY
                     PIRLLSGGNVQKVLLGREINSSPQLLITAYPVRGLDINSSYVIYDMLNEQKKKGVAVL
                     YIGEDLNVLLELCDRIMVICGGRIMGMVEPKDVTIEDLGLMMTGHTYSQILAERGTGK
                     "
     gene            480..1562
                     /locus_tag="PLI11_06395"
     CDS             480..1562
                     /locus_tag="PLI11_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52528.1"
                     /translation="MTLPFHIVKRPYMKTYQQVIVRLLAVLTAFAVTSILMLLLKKNP
                     GKVLMALLYEPVKNGYFLVQTIKLAVVLLIISLGIGLAFKMKFWNIGAEGQIILGGIG
                     ASYFGLFHSDWPIYILLPVMLVASFVTGGMYGLIPAYFKARYNTNETLFTLMLNYIAL
                     SFLLFLQYGPWKGANAMRPEFDNFKKNALLPSLSSSKPWSDLHIGWILSLVMVAVVYI
                     YMNHTKSGYEISVVGESVNTARYAGMNVRWITLRTMMLSAGICGIAGFVQVSGNLKKL
                     SVNVAGGLGFTAIIIAWLSKLNPVVMIVASFLFSVMSKGAGFLEYQNISPAASEMIQG
                     IILFCVLGAEFFVNYKIVRTNREVAK"
     gene            1562..2500
                     /locus_tag="PLI11_06400"
     CDS             1562..2500
                     /locus_tag="PLI11_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52529.1"
                     /translation="MIIASYIYIAVRVSIPVLLGITGAIFNEKAGNTNLGIEGMMYMG
                     AFAGFYTAVATNSVIMTLIAACLFGAFGALIYAFATVSLRTNQVVAGLSLTIFGQGVA
                     RFLGNTLSGGKSESVRIPDAVTQKVSWKIPVLKDIPFLGRALFGYDMIIYLSIVLVVI
                     AYFYINKTRTGLNLRAVGENPAAADAAGINVSLYKYVHICIGGAFCGLAGAYIGVVYL
                     TTWGNNMIAGKGWIAVALVIFASWNPLKAIYGSLLFGITSLIALRFQDSPISPYLLEM
                     LPYLVTVIILVITSVKMKKENQQPASCGVNYFREER"
     gene            2575..2913
                     /locus_tag="PLI11_06405"
     CDS             2575..2913
                     /locus_tag="PLI11_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52530.1"
                     /translation="MKMAVAYIFGNDEAPGIIGMVRFYVLNDGYTRLDVDIKGLALKK
                     YSFNIKNGDSYILPDLVTSEGRARMSFYTKDISINDILAKKIYLEGDNKVVASGTIRK
                     VVYGKKSYSE"
     gene            3112..>3595
                     /gene="trpS"
                     /locus_tag="PLI11_06410"
     CDS             3112..>3595
                     /gene="trpS"
                     /locus_tag="PLI11_06410"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPZ52531.1"
                     /translation="MGDKAKKRIFSGVQPSGHLTLGNYLGALKNFAILQDEYDCIYCV
                     VDLHSLTVRQNPEELRQRSYSVLALYIACGLDPEKNILFIQSHVPAHSELAWVLGCYT
                     YMGELGRMTQFKEKSSKHSENINSGLFTYPVLMASDILLYNADLVPVGDDQKQHLELT
                     R"
BASE COUNT         1070 a          606 c          867 g         1052 t
ORIGIN      
        1 gacatggtcg acaatgttat attaaaagat tacaaaaggc aaaaaggact tctgttaagc
       61 agaaagcctg caagaaagac ggctttgaag atggtggagg atctggatat tgttacccag
      121 ggcgttcaat atccaataag gcttctgtcc ggaggaaatg tgcaaaaagt acttttgggc
      181 agggaaatca attccagtcc tcaacttttg attactgctt accctgtaag aggtcttgac
      241 ataaattcat cgtatgtcat ttatgacatg ttgaatgagc agaagaaaaa aggagttgcg
      301 gtactgtata ttggtgagga cctaaatgta cttttggagc tctgtgacag aataatggta
      361 atatgtggtg gtagaataat gggtatggtt gaaccaaaag atgtaacaat agaagatttg
      421 gggcttatga tgacaggaca cacatactcc cagatacttg ctgaaagggg gactggaaaa
      481 tgacacttcc ctttcatatt gtaaaacgtc cgtacatgaa gacgtaccaa caggtaattg
      541 taagattgct tgcagtattg acggcttttg ccgtgaccag catactcatg ctgttattga
      601 agaaaaatcc tggcaaggtt cttatggcac tactgtatga gcctgtaaaa aacggatatt
      661 ttttagtgca gacaataaag ctggctgtgg tactgcttat aatttccctg ggaatagggc
      721 ttgcgttcaa gatgaagttc tggaacatag gtgccgaggg acagattatc ctgggaggaa
      781 taggagcttc atattttggc ttgttccata gcgattggcc aatatacatt ctgcttcctg
      841 taatgcttgt agcatcgttt gtaacaggag gcatgtacgg tctgataccc gcatatttca
      901 aggcaagata caatacaaac gaaactttgt ttaccctcat gctcaactat attgcgctta
      961 gttttctgtt gtttttgcag tatggtccat ggaagggagc gaacgccatg cggccggagt
     1021 ttgacaattt taagaaaaat gcgcttcttc catcactgtc gtcatccaaa ccttggagcg
     1081 atctccacat tggttggatt ttatcccttg tgatggtggc tgttgtatac atttacatga
     1141 accatacaaa gagcggatat gaaataagtg tggttggaga gagtgtcaac actgccagat
     1201 atgcgggaat gaatgtaagg tggataactt tgagaacgat gatgctgagt gctggaatat
     1261 gtggtattgc aggttttgtg caggtttcag gaaacctcaa aaaactgtct gtgaatgttg
     1321 caggaggact tggttttaca gcaataatta ttgcatggct cagcaaacta aacccggtag
     1381 tcatgattgt agcgtcattt ttgttcagtg taatgtcaaa aggagcaggt tttctagagt
     1441 atcagaacat atctccagcc gcctccgaga tgatacaggg tatcatactg ttttgcgtgc
     1501 ttggagctga gttctttgtt aactacaaga tagttcgtac caacagggag gtggctaagt
     1561 aatgattatt gcatcatata tctacattgc ggtaagagtg tcgatacctg tacttcttgg
     1621 tataaccggc gccatattca atgaaaaggc tggaaacaca aatcttggta ttgaaggcat
     1681 gatgtacatg ggagcttttg cgggcttcta tacagcagta gccaccaata gtgtgataat
     1741 gactctgatt gcggcttgtt tgtttggtgc ttttggagca ttgatatatg cttttgctac
     1801 tgtttcacta agaacaaatc aggtggttgc gggcttgtcc ctgacaatat tcggacaggg
     1861 agttgccaga tttttgggca ataccctgag tggcggcaag agtgaaagcg taaggatacc
     1921 ggatgcggta acacaaaagg taagctggaa gattcctgtt ctgaaagaca ttccgtttct
     1981 cggaagagcc cttttcggct atgacatgat tatttatctg tcaattgtat tggttgtgat
     2041 tgcgtatttc tacatcaaca agaccagaac aggcctaaat ttgagagcgg tgggcgaaaa
     2101 tcctgcagca gcggatgcgg cgggtataaa tgtttcattg tacaaatatg tacacatatg
     2161 cataggtggt gcattctgtg gacttgccgg cgcatatatt ggcgttgtgt accttactac
     2221 ctggggcaac aacatgattg ccggaaaagg atggattgcg gtggcattgg tgatatttgc
     2281 gagttggaat ccgctcaagg cgatttacgg ttctttgcta tttggcatta cctcacttat
     2341 tgccctgaga ttccaagaca gtccaatatc gccgtatctg cttgagatgc tgccatatct
     2401 ggttactgtt ataattctgg taatcacatc tgtcaaaatg aagaaggaga atcagcaacc
     2461 tgcttcctgt ggagtcaact acttcaggga agaaagataa ttggaaaata ttcaaacagt
     2521 attccccgat tattcataac ttatatagta gagagtaatt gggggtggaa aaacgtgaaa
     2581 atggctgtag catatatttt tggaaatgac gaagcaccag gaataatagg aatggtacga
     2641 ttttatgttc tgaatgatgg ctatacaaga ctggacgttg acattaaggg gcttgctctt
     2701 aagaagtaca gtttcaacat aaaaaacggc gattcttata ttttgcccga ccttgttacc
     2761 agcgagggca gggcgaggat gtcgttttat acaaaagata ttagtattaa tgacattctg
     2821 gctaaaaaaa tatatcttga aggagacaac aaggttgttg caagcggaac aataaggaaa
     2881 gttgtctatg gaaagaaatc atattctgaa taataaaacg gctcggtaat ggattgttta
     2941 tcttttatcg agcttttagt tatatatttg ttgtatactt gttgacaaga aagaaaaata
     3001 gtgtaaaatg caacattatc gaacatatat tcgaaaaacc atggttgaaa aaaatcatat
     3061 aaaatgctaa actttataag ttaactaata ataaaacagg aagtgttatg tatgggagat
     3121 aaggcaaaaa aaagaatatt cagtggtgta caaccttcag gacatttgac attgggaaac
     3181 tatctgggcg cacttaagaa ttttgccata ttgcaggatg aatatgattg catatactgt
     3241 gtggttgact tgcattcact gacagtcagg cagaaccctg aggaactgag gcaaagatca
     3301 tactcggtgc ttgcattgta cattgcctgt ggattggatc ctgaaaagaa tattctgttc
     3361 attcagtcgc atgttcctgc ccacagtgag ctggcatggg ttcttggatg ttacacatac
     3421 atgggtgaac ttggcagaat gacacagttc aaggaaaaat cgtcaaaaca ctcggagaat
     3481 ataaatagcg ggttgtttac ttatcccgtt ttgatggcat ccgatatcct tttgtacaat
     3541 gctgatttgg tgcctgtagg agacgaccag aaacagcatc ttgagcttac aaggg
//
LOCUS       DAONVY010000212         3559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18220_length_3559_cov_2.500285, whole genome shotgun sequence.
ACCESSION   DAONVY010000212 DAONVY010000000
VERSION     DAONVY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18220_length_3559_cov_2.500285"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLI11_06415"
     CDS             <1..742
                     /locus_tag="PLI11_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPZ52532.1"
                     /translation="LETTLRELKTDYVDLYQFHNPPFCPKPDDGTGLYEEMLKAKEKG
                     YIRHIGITNHRIKVAKEAIESNLYDTLQFPFSYLASDSEMELVNECIDNNMGFIAMKA
                     LAGGLIQNSAAAFAFMMQYDVLPIWGIQKLEELEEFVSYIDQPPKYEEYTHIIEKDRD
                     ILTGNFCRGCGYCLPCPSNIDIPNCARMSLMIRRAPTTVYMSKDWIDKMKAIEDCTEC
                     NSCKERCPYGLDTPNLLKENYKDYMTFV"
     gene            757..2172
                     /locus_tag="PLI11_06420"
     CDS             757..2172
                     /locus_tag="PLI11_06420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPZ52533.1"
                     /translation="MIFASLHFVYLYLPLFLLIYFLSSNIKWRNAVLLLFSLVFYSWG
                     EPKLLILILICSFVCYLAGLFMHATQNTRKRRIYLTISTSVVLGMLAFFKYWAFLVGN
                     INYVLKTQFPLWEIALPLGISFYTFQALTYVIDVYRHEVRVQKSFSKFLLYISMFPQL
                     VAGPIVTYSHVAEQIDRRVVSPDKFAKGVVRFVTGLGKKVIIANTLGELSSLLIEGEN
                     LYKTSVVGAWVGIFSYALQIYYDFSAYSDMAVGLGSMLGFDYPENFDYPYISSSVTEF
                     WRRWHITLGSFFRDYVYIPLGGNRKHQFFNLFLVWFLTGLWHGASWNFALWGLYFFVF
                     LYIEKKFLLRVFKRVKIIGWIFTSFVVLMGWVIFYYTDTSSLTYMFKVLFGFSDRPVN
                     DLFINLTINEYFYLLMAASVGCMPIARVVKRGLNNMYNSGRVKMEFAFIATCIFVLLV
                     LFTSSAMLVAKSYNPFLYFRF"
     gene            2183..>3559
                     /locus_tag="PLI11_06425"
     CDS             2183..>3559
                     /locus_tag="PLI11_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HPZ52534.1"
                     /translation="MTRRHALSITVLFVVFIFSLMIVSLTDSDREASYKENRTLAQRP
                     KLTVKSLLDGSFAKEFEEYFQDQFPFRDFFIDTSFKFTTLMARKGEDDVSLVEGGEGA
                     DYDENTNIFNPPSGFEPIEPTPSPEPTVEPTMTPGSPQATPGEQTPTTTPAPTKTPVP
                     TPAPTTDISYEYNQKFLRIGDRILEVYWYSKQRAESYFNVLKRVADELPNAKIYALLT
                     PSSVELYTPEKYKGYGHSSKEAIDYLFSNMDKRINQVNVYDKMMSKKNEYIYFRTDHH
                     WTQRGAYYAYEEFCKAANIKARKMNEYKKDTVPNFLGSYFKYIPDDRFRRNPDYLELF
                     YTLANNEGRAYYDISMNLKNSFAVKAVRTPNEMKDYPAKYMVFMNGDNPLTHFKTGVD
                     NNKRIVVFKDSYGNSLVPFLLSHYEDVYVIDIRAEKYLSGVLEFIKQKGINEVLFVNY
                     VNCPTQSDA"
BASE COUNT         1056 a          664 c          770 g         1069 t
ORIGIN      
        1 cctggagacg accttaaggg aactcaaaac agactatgtt gacttgtatc agtttcacaa
       61 tcctcctttt tgcccaaaac ctgacgatgg aacaggtttg tatgaggaga tgctgaaggc
      121 aaaagaaaaa gggtatatca ggcatatagg tattacaaat cacaggatta aggttgccaa
      181 agaggcgatt gaatcaaacc tgtatgacac attgcagttt cctttcagct accttgccag
      241 tgacagtgag atggaactgg ttaacgaatg tattgacaac aatatgggat ttattgccat
      301 gaaagctctt gcaggaggcc ttatacaaaa cagtgctgcg gcctttgcct tcatgatgca
      361 gtatgatgtt ttacccatct ggggtataca aaagcttgaa gaacttgaag agtttgtttc
      421 atatatcgac caacctccca agtatgagga atacacgcac atcattgaga aggacaggga
      481 tatattaaca ggcaatttct gcagaggctg cggatactgc ctcccttgcc cttcgaatat
      541 tgatattcca aactgtgcac gaatgtcgct tatgataaga agggcaccga ctacagtata
      601 tatgtcaaaa gactggattg acaaaatgaa ggcaattgaa gactgtaccg aatgcaacag
      661 ctgcaaggaa agatgcccat atggacttga tacgccaaat cttttaaagg agaactataa
      721 ggactacatg acatttgtct gaaacggggt ataattttga tatttgcaag tctacatttt
      781 gtatatctgt accttccttt gtttttgctt atatactttt tatcaagcaa tatcaagtgg
      841 agaaatgcgg ttttgctcct tttctccctt gttttctact catggggaga accaaaactg
      901 cttatcctta ttctgatctg ctcttttgta tgctatctgg caggtctttt tatgcatgca
      961 acccaaaata caagaaagag aaggatatac ttaacaatat ctaccagtgt tgttcttgga
     1021 atgcttgcat tcttcaaata ttgggccttc ctcgtaggga acataaacta tgtactgaag
     1081 acccagtttc cactttggga aattgcactt ccgcttggaa tctcattcta tacattccag
     1141 gcactgacct atgtaattga cgtgtacaga catgaggtac gggtgcaaaa gtctttttca
     1201 aagtttcttt tatacatttc catgttccct cagcttgtgg caggacctat tgtaacgtat
     1261 tcgcatgttg cagagcagat tgaccgcaga gttgtgtcgc ctgacaaatt tgccaagggt
     1321 gttgtcaggt ttgttacagg tcttggcaaa aaagtgataa ttgcaaacac attgggagag
     1381 ttgtcttctc ttttgataga aggggagaat ctgtataaaa caagcgtagt tggggcctgg
     1441 gtcggaattt ttagctatgc actgcaaatc tattacgact tttctgcata ttcagacatg
     1501 gcagtgggtt tgggttccat gctaggattc gactaccctg agaacttcga ctatccatat
     1561 atctcctcat cagtcacgga attctggaga agatggcaca ttacgctagg ttcttttttc
     1621 agagactatg tttacattcc actgggaggc aacagaaaac atcagttttt taaccttttt
     1681 cttgtctggt ttctaacagg tctttggcat ggagccagct ggaattttgc tctttgggga
     1741 ttgtatttct ttgtattttt atacattgag aagaagtttt tgttgagagt gtttaaaaga
     1801 gtgaaaataa tcggatggat ttttacatcc tttgttgtat tgatgggctg ggtcatcttc
     1861 tactacactg atacttcttc actgacttac atgtttaaag ttttgtttgg tttttcagac
     1921 agacctgtta atgacttgtt tatcaacctg actattaatg aatacttcta tttgctaatg
     1981 gctgcttccg ttggctgtat gcctatagca agagttgtca aaaggggact taacaacatg
     2041 tacaacagtg gcagggtgaa gatggaattt gcatttatcg ccacatgcat ctttgttctg
     2101 cttgttttat ttacaagcag cgcaatgttg gtggcaaaga gctacaatcc attcctgtat
     2161 ttcagattct aggaggactt gcatgacaag aagacacgca ctatccatta ctgtattgtt
     2221 tgtagtattt atcttttcat taatgattgt tagtttgacg gacagtgaca gagaagcatc
     2281 atacaaggaa aacaggaccc ttgcacaaag gccaaaactg actgtcaagt ctttgctgga
     2341 cggaagtttt gcaaaggaat ttgaggagta ctttcaggat cagtttcctt ttagggattt
     2401 cttcatagac acaagcttta aattcacaac tctaatggcc aggaaaggcg aggatgacgt
     2461 gtccttggtt gaaggcgggg aaggggcaga ttatgatgaa aacacaaata tttttaatcc
     2521 tcccagcggg tttgaaccaa ttgagcccac ccctagtccg gaaccaactg tggagccaac
     2581 aatgacacct ggcagtcctc aggctacgcc cggcgagcaa acaccaacaa ctacaccagc
     2641 accaacaaaa actccagttc caactccagc tcctacaacg gatatcagtt atgagtacaa
     2701 ccagaagttt cttaggatag gagacaggat tcttgaagtt tactggtatt caaaacaaag
     2761 ggccgaaagc tatttcaacg tattaaaaag agttgcagat gaacttccaa atgcaaagat
     2821 atatgcgctt ctgacgcctt cttccgtaga actttatact cctgagaagt ataaagggta
     2881 tgggcattca tcaaaggaag cgattgatta tcttttttca aacatggaca agcgaattaa
     2941 tcaggtaaat gtgtatgaca aaatgatgag taagaagaat gagtacattt actttaggac
     3001 tgaccaccat tggactcaaa gaggtgcgta ttacgcctat gaggagtttt gtaaagctgc
     3061 aaatatcaag gcaaggaaga tgaatgaata caaaaaggat acggtgccga attttcttgg
     3121 ctcatacttt aagtatattc ctgatgacag gtttagaaga aatcctgact atttagagct
     3181 tttctatact cttgcgaaca atgaaggcag agcgtattac gatatatcga tgaatctgaa
     3241 gaattcgttt gctgttaagg cggtgagaac accgaatgaa atgaaggact atcctgccaa
     3301 gtatatggtg tttatgaatg gcgacaatcc tttgacgcat tttaaaacag gagttgacaa
     3361 caataaaagg atagttgtgt tcaaggattc ctacggcaac tccttggttc cgttcttatt
     3421 atcccactat gaggatgtat atgtaattga tatcagggca gagaaatact tgagtggtgt
     3481 tttggagttt ataaaacaga aaggcatcaa tgaggtttta ttcgtaaact acgttaactg
     3541 cccaacccaa agtgatgcc
//
LOCUS       DAONVY010000213         3546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18310_length_3546_cov_3.746205, whole genome shotgun sequence.
ACCESSION   DAONVY010000213 DAONVY010000000
VERSION     DAONVY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18310_length_3546_cov_3.746205"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /gene="alaS"
                     /locus_tag="PLI11_06430"
     CDS             complement(<1..1648)
                     /gene="alaS"
                     /locus_tag="PLI11_06430"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPZ52535.1"
                     /translation="MKNYGLNELREKYLSFFESKEHLRLPSFSLIPKNDPSLLLINAG
                     MAPLKNYFMGIETPPSKRITTCQKCIRTPDIDNVGKTARHGTFFEMLGNFSFGDYFKK
                     EAINWAWEFLTEVLEIPKEKLYASVYLEDDEAYDIWTKEIGLPEEKVVRLDKDSNFWE
                     LGTGPCGPCSEIHFDRGEKYGCGKVDCTVGCDCDRYIEIWNLVFTQFDKQVDGTYEKL
                     PNPNIDTGMGLERLACVMQGVDNLFEVDTVRRILDTVCKMAGKEYGKDYKTDVSIRVI
                     TDHIRSTVMMVSDGVIPSNEGRGYVLRRLLRGAARHGRMLGIEGIFLCNLADTVVDES
                     KEAYPELETNRKYIKQIIQAEEDRFASTINQGLSMLEEIINEKNEQNDKSPLSGETAF
                     KLHDTYGFPLSLTQDIMSEHGFDVDVDGFRKEMEAQKEKARAAIMNRESSWQMGDAKA
                     LSKYPKTEFVGYDVDECTSNILAIFAKSDEGMEEVEQADSGMSVIILTEKTPFYAESG
                     GQEGDIGVISTETGLVKVLDTVKTDDRWRHIGVVEDGVVTK"
     gene            1827..2249
                     /locus_tag="PLI11_06435"
     CDS             1827..2249
                     /locus_tag="PLI11_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HPZ52536.1"
                     /translation="MLLEDLKSSEEICVVKYANYASLAQDKQLKTLFENHAKLEQQHL
                     NTVNSLLSGQIPPMSQQNNVQQPQGDENASLSISDKELCTDMLMTEKHVSSTYNTAIF
                     EFADKRVRDILNHIQKEEQQHGEAIFKYMETKGLYQPQ"
     gene            2350..2685
                     /locus_tag="PLI11_06440"
     CDS             2350..2685
                     /locus_tag="PLI11_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52537.1"
                     /translation="MKLVVVVLSEDSRLQALLQGLVENGIKGATIISSQGLARALEDA
                     EDYSFLGSIKAFLDPQQYGSTVFSVIQDNQVELVSKAIKQTVGDLSQPDMGIMFTIPV
                     DYVEGIRFD"
     gene            complement(2682..>3546)
                     /gene="purD"
                     /locus_tag="PLI11_06445"
     CDS             complement(2682..>3546)
                     /gene="purD"
                     /locus_tag="PLI11_06445"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPZ52538.1"
                     /translation="DPAVAVVVDDVADGLALGKGVIIAHDFESAEYAIVSMMENKKFG
                     ASGTKIVIEEYLTGPEVTVLAFTDGKTLVPMVSSQDHKRAYDNDEGPNTGGMGAFSPS
                     PVYSEEVAEICMRDIFMPTLEALNKEGIVYKGVIYFGLMITKDGPKVIEYNARFGDPE
                     AQCVLARLDSDLYEIFDAIVERRLDGMEIKWKKEKACCVVLASGGYPGKYETGYEIFG
                     VEQAERVGAYVFHAGTGGIDKCVTAGGRVLGVTALGKTLEEAILRAYKASAHISFKDM
                     YYRKDIGRKFV"
BASE COUNT          966 a          851 c          644 g         1085 t
ORIGIN      
        1 cctttgtaac aacgccatcc tcaacaacac cgatatgcct ccatctgtcg tcggtcttga
       61 cagtatccag aactttcacc aatcctgttt ctgtggatat gacaccaata tctccttcct
      121 gtccaccgct ttcagcataa aatggagtct tctcagtcaa gattataacg ctcatgccgc
      181 tgtctgcctg ctcaacttct tccatgcctt catcagactt tgcaaaaatg gcgagtatgt
      241 tggatgtgca ttcatccacg tcatatccta caaactctgt ctttgggtat ttgcttaaag
      301 ctttagcatc tcccatctgc caagagcttt ctctgttcat tatagcggct cttgccttct
      361 ccttttgagc ctccatttcc tttctaaaac cgtccacgtc tacgtcaaaa ccatgttctg
      421 acataatatc ctgtgtgaga gacaaaggaa atccataagt gtcatgcagc ttgaaggcgg
      481 tttctccact taaaggagac ttgtcatttt gctcattctt ctcatttatt atttcttcaa
      541 gcatggaaag gccctgattg atggtgcttg caaatctatc ttcctctgcc tgaattatct
      601 gcttgatata ttttctattg gtctcaagtt ctggataggc ttccttggac tcgtccacaa
      661 ctgtatcagc caaattgcat aggaagatgc cttctatacc cagcatgcgt ccatgacggg
      721 cagcacctct taaaagcctt ctaagcacat atcctctgcc ttcgtttgaa ggaataacac
      781 catcggacac catcattaca gtgcttctaa tgtgatcggt gataactctt atggaaacat
      841 cggtcttgta gtcctttcca tactccttgc ctgccatttt acagaccgta tccagtattc
      901 ttcttactgt atcaacttca aacaggttgt ctacaccctg cattacgcat gcaagccttt
      961 caagtcccat tcccgtgtca atattgggat ttggaagctt ttcatacgtt ccgtcaacct
     1021 gcttgtcaaa ctgggtgaat acaagattcc aaatttcaat atatctatca cagtcacatc
     1081 ctaccgtgca gtccacttta ccacatccat acttttctcc acggtcaaaa tgtatttcag
     1141 agcatggacc gcatggacct gttcccaact cccagaagtt tgagtcttta tcaagacgga
     1201 caaccttctc ttcaggaagt cctatttcct ttgtccaaat gtcgtaagct tcgtcatcct
     1261 caagataaac ggacgcataa agtttctctt tgggaatttc caatacttca gttagaaact
     1321 cccatgccca gtttattgct tcttttttga aatagtcgcc aaaagagaag ttgccaagca
     1381 tctcaaagaa agttccatgt ctagcagttt ttcccacatt gtctatatca ggagttctta
     1441 tgcacttctg acatgtggtt attctttttg atggtggagt ttctatcccc ataaagtagt
     1501 ttttgagcgg tgccattccc gcatttatta ataaaaggct tgggtcattc tttggtataa
     1561 gtgaaaagct aggtaatctt aaatgctcct tgctttcaaa aaatgataaa tatttttctc
     1621 tcaactcgtt taatccataa ttcttcatgc tatctgtatc tcctttatct taagctttca
     1681 agctattgaa taattatatt accacaactt gtttatgtca attattgcaa cgattttggc
     1741 aatttccatg gataatattt gaacaattac aaatactatt gcaggaggtg tttaaattga
     1801 caataacttt aactcaaaaa gaacgaatgc tgcttgaaga tttgaagagc agtgaggaaa
     1861 tctgtgtagt aaagtatgca aattatgcaa gccttgcaca ggacaagcaa ttaaaaacac
     1921 tgtttgaaaa ccatgcaaaa cttgaacagc agcatttaaa tacggtcaac agccttttga
     1981 gtggacagat tccgccaatg agtcaacaga acaacgtaca gcagccgcaa ggagacgaaa
     2041 atgcgtctct atcaatttct gacaaggagc tctgtacgga catgttgatg acggaaaaac
     2101 atgtctccag cacttacaat accgccattt ttgaattcgc cgataaaagg gtgagagata
     2161 ttctcaacca tatccaaaag gaagagcagc agcacggtga agccattttc aagtatatgg
     2221 agacaaaagg cttgtaccag ccacagtaaa tatgggaagg ttctaccctt cccattacat
     2281 aaacttattg acaaccagca aataagtgtc atattataca atcataagaa atttcaggca
     2341 ggtgtatata tgaaactggt tgttgtcgtt ctcagtgaag attcaaggct acaggctctg
     2401 cttcaaggct tagttgaaaa cggtatcaag ggagccacaa ttataagttc ccagggattg
     2461 gcaagggctt tggaggatgc cgaagattat tcttttcttg gctctattaa agcttttctt
     2521 gatccgcagc agtatggcag cactgtcttt tccgttatac aagataatca ggttgaactg
     2581 gtctcaaaag ccataaaaca gactgttggc gacctgagtc aacctgatat gggtataatg
     2641 ttcactattc ctgttgatta tgtggaaggc attagattcg actagacaaa cttccttcca
     2701 atatcctttc tgtagtacat atccttgaaa gatatgtgcg cagatgcctt gtatgccctt
     2761 aaaatggctt cttccaaggt tttaccgaga gccgttactc cgaggacccg gccacctgct
     2821 gtcacgcact tgtcaatgcc gccggtgccg gcatggaaca cataggctcc cactctttcg
     2881 gcctgttcaa ctccgaagat ttcatatcct gtttcatact tcccgggata ccctccggat
     2941 gcaagaacca cacaacaggc cttttccttc ttccacttta tttccatgcc atcgagtctt
     3001 ctctcgacaa tggcatcaaa tatctcatac agatcgctgt caagtctggc aagaacgcat
     3061 tgagcttctg gatctccaaa tcttgcattg tattcaataa cctttggacc atccttggtt
     3121 atcatcaggc caaaatatat tacgcctttg tatacaatgc cctctttatt caacgcctcc
     3181 aatgttggca tgaagatgtc tctcatgcag atttctgcaa cttcctccga atatacaggg
     3241 cttggagaga acgctcccat tccaccggta tttgggccct cgtcattgtc ataagctctc
     3301 ttatgatcct gtgaggatac catgggaacc aatgtttttc catcggtaaa agcaagcacg
     3361 gtcacttcag gtccagtcag atactcttca ataactatct ttgtacctga tgcgccaaac
     3421 ttcttgttct ccatcattga gacaattgca tattctgcgc tttcaaaatc gtgcgcaata
     3481 atgacacctt tacccagggc aagaccgtct gctacgtcgt caacaacgac ggccaccgct
     3541 gggtca
//
LOCUS       DAONVY010000214         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18320_length_3545_cov_3.456447, whole genome shotgun sequence.
ACCESSION   DAONVY010000214 DAONVY010000000
VERSION     DAONVY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18320_length_3545_cov_3.456447"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLI11_06450"
     CDS             <1..554
                     /locus_tag="PLI11_06450"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52539.1"
                     /translation="LNRREKAYLEIMSEMRRQGIAERPIVLGAEVSIERDLQYAQGVE
                     KLCIEGTDIILFELPYSRLKGWEVSVIENIIFKHRLRPMIAHVDRYLDVFEVSDFMQM
                     YQIPDVIFQVDASCVDKFRYRSFLMKLIKKGSRVVLGSDCHNMGSRKPEYIKGIQYLG
                     KKLDKESFNKLLYNMRDILNITL"
     gene            569..1084
                     /gene="loaP"
                     /locus_tag="PLI11_06455"
     CDS             569..1084
                     /gene="loaP"
                     /locus_tag="PLI11_06455"
                     /inference="COORDINATES: protein motif:HMM:NF033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HPZ52540.1"
                     /translation="MTTNWYVIQVPSKKERLVCERIKDILDKDIYIDCFVPLAERVYK
                     KDGKVKIVTRPLFPGYLFIISDEIEQVYLQIKKVPYFTRVLHADFQFVPLKRNELEVF
                     VDFLNQERIMQMSKGYIEGTQVFVTDGPLKGQEGRIKKINRHKRIAIIETPFMGKTTT
                     IHVPLEIVSKI"
     gene            1182..2804
                     /locus_tag="PLI11_06460"
     CDS             1182..2804
                     /locus_tag="PLI11_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPZ52541.1"
                     /translation="MKKVLSIIMVVILVLQVSCVKKPENKGNNDDFKEVKISVYKASE
                     ILETINKVNTYFQEKLTASSNNWDDAVYHSGNIRAYMYTGNLDFYNYSLEYAKRHHYL
                     VNKGQNTVNGDLYCISQTYIDLYKLKQEDYILADVIRNADFNADGRTDFGWIDLVYMS
                     LPVFAELSEITKDTKYIDDAYRAYKKARDIMWDEEASLFYRDARFVYDKNKSGTMTPS
                     GKKILWSRGNGWAFAGLAKTLEVLDKNHESYKLYLKDFKAMAESLKGRQREDGTWNAN
                     LDDPEHFGGIETSGTVMFMYGYAIGIKKGFLKYDEYFDTLKKAYDGVVKHAISDEGRL
                     LYVQPVSDSPQNYQNYHDEEARKQSTKQYAVGIFVMAACEIMSMCEDYEKPELVIPED
                     DYEPISQEDRAIDPNYYKGKIKVTATNQQEGNEAEKLVDGVTEDKDGHRWSAPGFGSS
                     ATLEFEEVLSIKKIVVVPYQNRAYRYVIEKSMDGKDFEMVVDYRKNDKEWFFFSHDVD
                     IEAKYIRIRVTGAYNYTGAWVSINEMLVYTKD"
     gene            2821..>3545
                     /gene="kduI"
                     /locus_tag="PLI11_06465"
     CDS             2821..>3545
                     /gene="kduI"
                     /locus_tag="PLI11_06465"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HPZ52542.1"
                     /translation="MDVRYGANAKDVKRYTTKELREEFLIEKVFVPNDITSVYSHIDR
                     ILIMGCMPTEDRIAINKNFDAWKSFGVEYLLQRRELGIINIGGKGKVIVDDKEHMMGR
                     FDCLYVGMGAKEIYFESYDKNDPAKFYMASTPAHKKCPDKYISLESAAKNPMGSPKTS
                     NERVINQYIHPSVLETCQLSMGLTILKEGSVWNTMPAHTHERRMEVYIYFDIQEDNVV
                     FHFMGEPDETKHIVVKNEQAVISP"
BASE COUNT         1257 a          586 c          811 g          891 t
ORIGIN      
        1 tcttaaatag aagagaaaaa gcgtatctgg aaattatgtc agagatgcgc aggcaaggta
       61 ttgctgaaag acctattgtg cttggtgctg aagtgagcat agaaagagat cttcagtatg
      121 cgcaaggtgt agaaaaactg tgcatcgagg gtactgacat aatactgttt gagctgcctt
      181 acagcagact taaaggctgg gaagtcagtg tcatagaaaa cataatcttc aaacatagat
      241 taagaccaat gattgcccat gtggacagat atcttgatgt gtttgaagta agtgatttta
      301 tgcagatgta ccagattcct gatgtaatat tccaggtaga cgcatcctgt gtcgacaagt
      361 tcagatacag gtccttcctt atgaaactca taaagaaggg cagccgtgta gtattgggca
      421 gtgattgcca taatatgggt tccaggaagc cggaatatat aaaaggaata caataccttg
      481 gcaaaaaact tgataaagag tcctttaata agttattata taacatgcga gatatactaa
      541 acatcacgtt atagaggtta gagcataaat gacgactaac tggtatgtaa tacaagtacc
      601 atcgaaaaag gaaagacttg tatgtgaaag aataaaggac atattggata aggacatata
      661 cattgattgt tttgtgccac tggcagaaag agtatataaa aaagatggaa aggtaaagat
      721 agtaaccagg cctttgtttc ctggttactt gttcataata agtgatgaga tagaacaagt
      781 atatcttcag ataaagaaag taccatattt tacaagagtg cttcatgcag actttcaatt
      841 cgttccactc aaaagaaatg aattggaggt ttttgtggac tttttaaacc aggaaagaat
      901 aatgcaaatg tccaaaggct acatagaagg tactcaggta tttgtaactg atggacctct
      961 caagggccag gaaggaagaa taaaaaagat aaaccgccat aagaggatag cgataattga
     1021 gacccccttc atgggcaaga ctacaacaat tcatgtaccg ctggagatag ttagcaagat
     1081 ataagttgaa tatacacaaa aatacgttga gtttacatac tttaccgttt gctattggta
     1141 tatagtatgt atattaggct attccaacgg gaggtccagt tatgaaaaag gtgctgtcca
     1201 taataatggt ggttattctc gttttacagg tttcctgtgt caagaaacca gagaataagg
     1261 gaaataatga tgattttaag gaagtaaaaa tctcagtcta caaagcctct gaaatactgg
     1321 agacaatcaa caaggtaaac acatattttc aagaaaaact cacagcaagt tcaaacaact
     1381 gggatgatgc cgtatatcac tccggcaaca taagagcata tatgtatacc ggaaatcttg
     1441 atttctacaa ctactctctt gaatatgcaa aacggcatca ttatcttgtc aacaaaggac
     1501 aaaacaccgt caatggtgac ctttactgta tctcacaaac ctacatagac ctgtacaaat
     1561 taaaacaaga agactatata cttgcagatg ttatcagaaa cgcagatttc aatgcggatg
     1621 gaagaacgga ttttggttgg atagatctgg tctacatgag tcttccagta tttgcagaac
     1681 tatcagaaat cacaaaggat acaaaataca tagacgatgc ataccgggca tacaaaaagg
     1741 cacgggacat aatgtgggat gaggaagcaa gtcttttcta cagggatgca agatttgtat
     1801 atgacaaaaa caaaagcggc acaatgacgc catcgggaaa aaagatcctg tggtcaagag
     1861 gaaatggatg ggcgtttgcc ggacttgcca agacccttga agtacttgat aagaatcatg
     1921 aatcatataa gttgtattta aaggacttca aggccatggc agaatccctt aaaggaagac
     1981 aaagggaaga cggcacttgg aatgcaaatc tggatgaccc tgaacatttt ggcggcattg
     2041 aaacctcggg taccgtaatg tttatgtatg ggtatgcaat aggtataaag aagggtttct
     2101 taaaatatga cgagtatttt gacactctga aaaaagccta tgacggagtt gtcaaacatg
     2161 ccatttctga tgagggacgg cttctttatg tacaacctgt atccgacagt cctcagaatt
     2221 atcagaacta tcatgatgaa gaagcaagaa aacaaagcac aaaacagtat gcggttggca
     2281 tttttgtcat ggcagcctgt gagattatgt ccatgtgcga ggactatgag aagccagagc
     2341 ttgttattcc cgaagatgat tatgagccca taagtcagga agacagagcc atagatccta
     2401 actactacaa agggaaaatc aaagtgactg ccaccaacca gcaggaagga aatgaagcag
     2461 aaaagctggt ggatggcgtt acagaagaca aggatggaca caggtggtcc gctccgggat
     2521 ttggaagctc cgccactctg gaatttgaag aagtcctgag tattaagaaa attgtggtgg
     2581 ttccatatca gaacagagct tacagatatg taatagaaaa gtccatggat ggaaaggatt
     2641 ttgaaatggt ggtggattac aggaagaatg acaaggaatg gttcttcttc agccatgatg
     2701 tggacataga agctaaatat atacgaataa gagtaacggg tgcctacaat tacacaggcg
     2761 cttgggtaag cattaatgaa atgcttgttt atactaaaga ttaatggagg ttttgaattg
     2821 atggatgtta ggtatggcgc aaatgcaaag gatgtaaaaa ggtacacgac aaaggaactt
     2881 agagaagagt tcctgatcga aaaggtattc gtcccaaatg acataacttc tgtatactcc
     2941 catattgaca gaatactcat aatgggatgc atgccaacag aagacagaat agcaataaac
     3001 aagaactttg atgcatggaa aagctttggt gttgagtatc ttttgcagag aagggagctt
     3061 ggaatcatca atattggagg aaagggcaaa gttatcgtag atgacaagga acacatgatg
     3121 ggcagatttg actgtcttta tgtgggaatg ggagcaaaag aaatatattt tgaaagctat
     3181 gacaaaaatg atcctgcaaa attctacatg gcgtcaacac ctgctcacaa gaaatgtcct
     3241 gacaagtaca tatcactgga aagtgcggca aagaatccaa tgggaagccc caagacatcg
     3301 aatgaaaggg ttataaacca atacatacac ccgtcggtgt tggagacatg ccagctttcc
     3361 atgggcctta caatattgaa ggaaggcagc gtatggaaca ccatgcctgc ccacactcat
     3421 gaaagacgca tggaagtata tatctatttt gacatacagg aagataatgt ggtgtttcac
     3481 tttatgggtg aacctgatga aacaaaacac atagtagtaa agaacgagca ggcggtaata
     3541 tctcc
//
LOCUS       DAONVY010000215         3543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18334_length_3543_cov_4.046445, whole genome shotgun sequence.
ACCESSION   DAONVY010000215 DAONVY010000000
VERSION     DAONVY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18334_length_3543_cov_4.046445"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLI11_06470"
     CDS             <1..924
                     /locus_tag="PLI11_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPZ52543.1"
                     /translation="IDVLKEVGEMSERLEYGDHEAQEYLEDDRVDTSALKQYLREIGK
                     YRLLEPKEVEDLFKRTRDGDKEAYNKLVVHNLRLVVKYAKIIKKTYKVEESIMDLIQA
                     GNIGLMRAVDKYEYKKGYAFSTYATWWIRQSIVRHIYDNEGTIRVPVHMSEKFIAISK
                     AIEAFKEQEGREPTFEELKEHVKLSEKDYKLYKTFDKNVASLNTTIGEDGDSELMDFI
                     SSDDSPDPVYEEAKNILLSDTVREVLEKLDKREGYIIQERFGLNGRTPKTLEEIGYDL
                     GLTRERVRQIESVAMKKLRTSAKASSIRAFA"
     gene            complement(990..>3543)
                     /locus_tag="PLI11_06475"
     CDS             complement(990..>3543)
                     /locus_tag="PLI11_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52544.1"
                     /translation="TSKDEWAISSASSAVIENGKLTCKSGRSFSIRPGFTIGDDYGLE
                     PGYFTFDLVMTGGQISIGTRLFSNVTIKGTQGLWFTLTKDNLVVEDPSTKLKETINHG
                     MDLSTEQNVRVLDNLDTIEFRINDTVICSVTIENEKLMLFDANGNELAAMKNETVPHA
                     GYLRMDVDKLQGSLDNVEYANRQITQKYVERHGRDIDYSTWFAVDDLGRKTPDNEEAG
                     NRKNEKYVGLFYFINHVHSSGGEIRDHTKIYLEQGLDALKAFLPNSPGGHWAEPYFGY
                     YLSTDEWIYRKHAAMFQAAGIDFIFLDMSNAQTFPESVKVLFDTWLDIRRKGGQTPQI
                     VPMCGDMPATLVIDMYTIKDTIFSIPEYEELLFKWDGKPLILGNNDNPEGDLWTVSGT
                     TPQTREQFYEVINSNSKIKQFYESGEFAEWLSKFTIRKCWAWQASRYEEDKSFAGYWD
                     WLDWHPQAPGRNFDGVIEQISVTMGTHAHTSTGRSYVGEKDYGNGLQDFDFSLETSKY
                     GLCFEQQFKHALKENPKVIMITGWNEWYAGVQKTQNKQQTTGHTLTPGYYLVDQFNPE
                     FSRDGEPMKLRTGVGFGDNYYYQMVNYIRQFKGMDKTPVASGQKTIDISKGISEWADV
                     GPEFRDHLNDTTFRRHLSWGGVYTYINNTGRNDLDYAKVSQDADNLYFLIATAHDLVT
                     VDEDNWMNLFINTDINHETGWEGYDFVLNRSRDGKTVSVEKFVDNDWDFEKVGDAEYF
                     MGGNFIAIKVSKSILGITEELASFDFKWADNSTKTGDVMQFMDMGDAAPDSRFAFRYL
                     GTAPATKKSSPNFFANIFNPANWTAVHLVVFSVIAATIIIVVAVILAKPKKK"
BASE COUNT          956 a          737 c          781 g         1069 t
ORIGIN      
        1 attgatgttc taaaggaggt tggcgaaatg agtgaaagat tagaatatgg tgaccacgaa
       61 gctcaggaat acttggaaga tgatcgtgtt gataccagtg cgcttaagca atatcttagg
      121 gaaattggca agtacagatt gcttgaaccc aaagaagtgg aagatttgtt taagagaaca
      181 agagatgggg acaaggaagc atacaacaag ctggtagtcc acaaccttcg tttggttgta
      241 aaatatgcca agataataaa gaagacgtac aaggtcgaag agtccattat ggatttgatt
      301 caggcaggaa acataggatt aatgcgtgct gttgacaaat acgagtataa aaaaggatat
      361 gctttcagca cttatgctac ctggtggatt agacaaagta ttgttagaca tatatatgac
      421 aacgaaggaa ccattagagt tcctgttcac atgagcgaaa agttcattgc aatttcaaag
      481 gctatagaag catttaagga gcaggaaggt agggaaccaa ccttcgagga acttaaagag
      541 catgtaaaac tttctgaaaa ggattacaag ttgtacaaga cctttgataa gaatgttgca
      601 tccctcaaca ccacaatagg cgaagacgga gatagtgagc ttatggattt catctcatct
      661 gacgattcgc cagatcctgt gtatgaagaa gcaaagaaca tattgcttag tgatacagtc
      721 agggaagttc ttgaaaaact tgacaagagg gaaggatata tcatacaaga gagatttggt
      781 ttgaacggca gaaccccaaa gacgctggag gagattggtt atgacctggg tctaaccaga
      841 gagcgtgtaa ggcagattga aagtgtggcc atgaagaaac tgagaacatc cgccaaggca
      901 agcagcataa gagcttttgc atagatttga aataaatatg gacaagcata aaaaacaggt
      961 gagcattcat gctcacctgt ttcttattct tactttttct tcggctttgc cagtataact
     1021 gctacaacta taattattgt tgctgcaatc acactgaata ccaccaggtg gactgcggtc
     1081 cagtttgcag ggttaaaaat atttgcaaag aagtttggtg aagatttctt tgtggcaggt
     1141 gcagtaccca aatatctaaa agcgaatctt gagtcgggag ctgcgtcgcc catatccatg
     1201 aactgcatta catcaccggt ttttgtggag ttgtcagccc acttgaaatc aaaggatgcc
     1261 agttcttcag ttatacccag tatgctcttt gataccttga ttgcgatgaa gttgccgccc
     1321 atgaagtatt ctgcatctcc aactttttca aagtcccaat cgttgtctac aaacttttca
     1381 actgaaacag tcttgccatc acggcttctg ttcagtacaa agtcataacc ttcccaaccg
     1441 gtttcatgat ttatgtctgt gtttataaac aggttcatcc agttgtcttc atcgacagtt
     1501 acaagatcat gtgcagtggc tatcaggaag tataaattgt ccgcatcctg cgaaaccttt
     1561 gcataatcaa gatcgtttct tcctgtgttg ttgatgtagg tgtacacgcc accccatgaa
     1621 aggtgccttc tgaaggtggt atcgtttagg tgatccctaa actcaggtcc tacatccgcc
     1681 cactcggaaa taccctttga tatgtcaatt gtcttctgtc cggaagcaac tggagttttg
     1741 tccattccct tgaactgcct tatatagttt accatctggt agtagtagtt gtctccgaat
     1801 ccaactccag tacgtagttt cattggttcg ccgtcacggc tgaattcagg gttgaattgg
     1861 tcaaccaggt agtaaccagg agtcagggtg tgtcctgtgg tctgctgctt gttctgtgtc
     1921 ttctgaacgc ccgcatacca ctcgttccat cctgtaatca ttattacttt aggattctct
     1981 ttaagagcat gtttgaactg ctgttcaaaa caaagtccat actttgatgt ttccagtgaa
     2041 aagtcaaaat cctgtagtcc gttgccgtaa tctttttctc ccacatagct tctgccagtg
     2101 ctggtgtgag catgggttcc catggtaacg gagatctgtt cgattactcc atcgaagttc
     2161 cttccgggtg cctgtggatg ccagtcaagc caatcccagt atcctgcaaa tgatttgtct
     2221 tcctcgtatc ttgaggcctg ccaagcccag cattttctta tggtaaactt ggataaccac
     2281 tctgcaaatt ctccgctttc atagaattgt tttatctttg aattagaatt aataacttcg
     2341 tagaactgtt ctcttgtctg aggtgttgtg cctgatacgg tccagagatc gccttctgga
     2401 ttatcgttgt ttccaagaat gagaggtttt ccatcccact tgaacagaag ttcctcgtat
     2461 tcaggaatag agaagatggt atccttgatt gtatacatgt caattaccaa tgtagcgggc
     2521 atgtctccgc acatgggcac aatctgtggt gtctggccac cttttcttct tatgtcaagc
     2581 caggtatcga aaagaacctt cacgctttca gggaaagtct gggcgtttga catgtcaagg
     2641 aaaatgaagt ctatgcctgc ggcctggaac atggctgcgt gctttctgta aatccattca
     2701 tcggtggaaa ggtagtaacc gaaatatggt tctgcccagt gtcctccagg tgagtttggc
     2761 aagaaagctt tcagtgcatc aaggccctgt tcaagatata tttttgtatg gtcgcggatc
     2821 tctccgccac ttgagtgaac gtggttgatg aagtaaaaga gacctacata tttttcattt
     2881 tttctgtttc ctgcttcttc attatcggga gtttttcttc ccagatcgtc cactgcaaac
     2941 caggtggagt aatcgatatc tctgccgtgg cgttctacat acttttgtgt tatttgtctg
     3001 tttgcatact caacattgtc caatgagccc tgtagcttgt ccacatccat tctcaagtag
     3061 ccggcatgtg gaacggtttc attcttcatg gctgcaagtt cgtttccgtt ggcatcgaag
     3121 agcataagtt tttcgttttc aattgttaca ctacaaataa cagtgtcgtt gattctaaat
     3181 tcaatagtgt caagattgtc aagaactctg acattctgtt ccgttgacaa atccataccg
     3241 tgattaatcg tttccttcaa tttagttgat ggatcctcta ccacaaggtt gtccttcgtc
     3301 agtgtaaacc aaaggccctg tgttccttta attgttacgt ttgaaaagag cctagtgcct
     3361 attgatatct gtcctcctgt catgaccagg tcgaaggtaa agtatcctgg ctcaaggccg
     3421 tagtcgtctc caattgtaaa cccgggcctt attgaaaatg accttccaga cttgcatgtc
     3481 agcttgccat tctctataac ggcactggag gctgaggata ttgcccattc atccttgctt
     3541 gtg
//
LOCUS       DAONVY010000216         3524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18436_length_3524_cov_4.093110, whole genome shotgun sequence.
ACCESSION   DAONVY010000216 DAONVY010000000
VERSION     DAONVY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18436_length_3524_cov_4.093110"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..806)
                     /locus_tag="PLI11_06480"
     CDS             complement(108..806)
                     /locus_tag="PLI11_06480"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPZ52545.1"
                     /translation="MKFNRKVMASISLWLTRITPFVAIILLAVIWYTDFRHLTIDDIL
                     SFTPDNPLTAFFVLMVLFALKSLVIFFPVTILYLSVGAIYPPHLALIINFIGIGVCVS
                     IPYFLGKLSGQEIIDKIITKYPKSKRYIAPDKSDSWMYSFLVRTVNVLPGDMRSMLLG
                     AVGVPFKTYISSSLVAAIPYLVSVTFVGAHMDNPDSPAFITALVFTGFVTIGSVVVYF
                     LFKKRKLRNNKDVV"
     gene            complement(931..1713)
                     /locus_tag="PLI11_06485"
     CDS             complement(931..1713)
                     /locus_tag="PLI11_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPZ52546.1"
                     /translation="MIKRATLVLLTLLLAVSAAACANSKSTGDYFADEEIIENTTGNS
                     TERKIIVKARYDIETEDLDKTVESFTKQVSELGGYIEYSSIRKDSADFTIRIPVGKLD
                     TFVNFAKDSGRVTYSSQHGKDVTIEYYDTQAELNALRTQEERLLDLLEQAQSLDDILK
                     LEEQLGTIRTKIEKLTTRLNQLNNLVNFATIEVSLEEVDSMETGFGALLTKTFKSSIN
                     AALNLVKYAIVVLVWLLPFLIPVVVIIAIVLIIKKIKTKPKN"
     gene            1860..2597
                     /gene="sigI"
                     /locus_tag="PLI11_06490"
     CDS             1860..2597
                     /gene="sigI"
                     /locus_tag="PLI11_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HPZ52547.1"
                     /translation="MREFDQLASLAKDDEEKLEQLIHKCEFFIMKTASKVSKRYISKE
                     DDEFQIALLGFSEAVQEYEMDKGSFFSYAELVMRRRLIDYFRSQTKYTAEFSVSPYVF
                     SSDVEETDMQVYKAVNESLVYEEDNTSHDIKDEIASLKEVLKKYGFSFMDLADCSPKA
                     EKTQKQCALACIFMLDNPMLVENLRTTKQFPLKIIQNNCNVSRKTLERHRKYLIAAVE
                     ILSGNFPYMKEYLRYIEEVRRNESGRS"
     gene            2578..>3524
                     /locus_tag="PLI11_06495"
     CDS             2578..>3524
                     /locus_tag="PLI11_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52548.1"
                     /translation="MKAVVLDIEEKHAILLNQDGTFVRVENKEYEIGQSVEFRPRTMR
                     FRYGLVACIALFVIVFGLGTFAYFDPYTYVSFDVNPSVEFTVNRFDRVIKVRVINEEN
                     EQSLDKAVFKDINHKRIDTAIKKTLEEVKESGYLSEESKVVIATSNPNKNKAQSLANR
                     LEEVVSKEEREVAVETVCVDKNKLDDAREYGITPGKLTLIEKLKDALGNEEFDINDWT
                     KKSVKEIVNATNEVKKNEKEKRIKPGDDPKNADDLEDEKEEVKEEKKDIKDAKKEEEK
                     QEKEEKKKQEKEEKEREKQERKEEKEKQKQEKKEEKEKE"
BASE COUNT         1217 a          577 c          754 g          976 t
ORIGIN      
        1 aagcaattct ataaaacatt gtgctatttt ttcatgttta gtttacaaag cctttaatat
       61 cggaagtata taatatttga ttcaaaatgg caagttgtgt ttaatattca cactacatcc
      121 ttgttgttcc taagttttct cttcttgaaa agaaaataca ctacaacgga acctattgtt
      181 acaaagcctg tgaacaccag cgcagttata aatgctgggg aatcaggatt atccatatgc
      241 gctcctacaa aggttacaga tacaagatat ggaattgctg ctaccagtga gcttgatatg
      301 tatgttttaa aagggacgcc aactgcgcca agaagcatgc ttctcatgtc tcccggaaga
      361 acattgacgg tacgaaccaa aaaagaatac atccatgaat cgctcttgtc cggggctatg
      421 tatcttttgg attttgggta tttggttatt attttgtcaa taatctcctg gcccgatagt
      481 tttccaagga aataggggat agaaacgcat actccgattc ctatgaaatt gataataagg
      541 gccaggtgtg gaggatatat tgcccccacg cttagataca gtatggtaac cgggaagaat
      601 atcaccaatg atttcagcgc aaaaagcacc ataagtacaa agaatgccgt taatggattg
      661 tctggtgtaa atgaaagtat gtcatctatt gtaaggtgtc tgaaatcggt gtaccaaatt
      721 actgccagca atataattgc aacaaaaggt gttattcttg tcagccataa ggatatggaa
      781 gccattactt tacgattgaa cttcattaca atctccttta tagaaacatt acaatactta
      841 attatatcat attcatatta ataaacaata acccgtgcaa acaaaagata tttgcacggg
      901 ctgtaataag gagatgtaaa aaaaaaccag ctaatttttt ggtttggttt ttatcttctt
      961 aatgattagt acaatggcaa ttataactac caccggtata aggaaaggta acagccaaac
     1021 cagaaccaca attgcatatt taacaaggtt taaagcagca ttgatagatg atttgaatgt
     1081 ctttgtcaac aatgctccaa aacctgtttc catgctgtca acctcttcaa gagagacctc
     1141 tatagtagca aagtttacta gattgtttag ttggttcaac ctggtggtta gtttttcaat
     1201 cttggttctt atggtgccta attgttcttc gagtttcagt atgtcatcga gcgactgagc
     1261 ctgctctaat agatcaagaa gcctctcctc ctgagttctc agagcgttca gttcggcctg
     1321 ggtgtcgtag tactcgatgg ttacgtcctt tccgtgttga cttgagtaag tcaccctgcc
     1381 ggaatccttt gcaaaattga caaaagtatc aagctttccg acaggtatgc gtattgtaaa
     1441 atctgcacta tccttgcgaa tactggagta ttctatatat cctcccagct cggaaacttg
     1501 ttttgtgaat gattccaccg ttttgtcaag gtcttcggtt tctatatcgt atcttgcctt
     1561 gacaataatt ttacgctcgg tagaatttcc tgtagtgttt tcaattattt cttcatctgc
     1621 gaaataatcg ccggtacttt tgctgtttgc acaagcagcg gcagaaacgg caagaagcaa
     1681 ggtcaataaa actagcgttg ctctctttat cataattaag accccatttt ttttgattat
     1741 atcagaccat ggaaaaaaat aaagatgctt tatgtaaatt atatgtgaaa atttaacagt
     1801 ttgacgatcg agcggaatga agtataatgt aaaagaactt aagtattgga ggaagcaagt
     1861 tgagagaatt tgaccaactg gcctctttgg caaaagatga cgaagaaaaa ctggaacaac
     1921 ttatacataa atgcgagttt ttcataatga aaacggcatc caaagtttcg aaaaggtaca
     1981 taagcaaaga ggatgacgag tttcagattg cccttttggg gttttccgag gctgtccagg
     2041 aatacgaaat ggataaaggt agtttctttt catatgccga gctggtaatg agaagacgac
     2101 tcatcgacta tttcagatct cagacaaaat atacagcgga gtttagtgta agcccgtatg
     2161 tcttttcttc cgatgtggaa gaaacggata tgcaggtata caaggctgta aacgaatccc
     2221 ttgtttatga ggaggataat acttctcatg atataaaaga cgagattgca tctctgaagg
     2281 aagttttaaa gaaatacggt ttttccttca tggacctggc agattgttcc ccgaaggcgg
     2341 aaaagactca aaagcagtgt gcgcttgcat gcatattcat gctggataat ccgatgttgg
     2401 tagagaattt gcgcacaaca aagcagtttc cattaaaaat tattcaaaat aactgcaatg
     2461 tatcccgaaa aacattggag cggcatcgaa aatacttaat agccgcagta gagatattgt
     2521 ccgggaattt cccatatatg aaagaatacc tgaggtatat agaagaggtg cgaagaaatg
     2581 aaagcggtcg ttcttgatat agaagagaag catgctatat tgttaaacca ggatggaacg
     2641 tttgtcaggg ttgaaaataa agaatatgaa ataggtcagt ctgtggaatt tagaccaagg
     2701 acaatgaggt tcagatacgg acttgttgcc tgcattgcat tatttgtaat tgtttttgga
     2761 ctgggtacct ttgcatattt tgacccttac acttatgtga gttttgacgt gaatccttct
     2821 gttgagttta cagtcaacag atttgacagg gtaatcaaag tccgagttat aaatgaagaa
     2881 aacgagcagt ctttagacaa agcagtattt aaagatataa atcataaaag aattgatact
     2941 gcaataaaga aaacccttga agaagtcaag gaaagtggct atttgtctga agaaagcaaa
     3001 gtggttattg caacttcaaa tcctaacaaa aacaaggcgc aaagtcttgc aaaccgtctg
     3061 gaagaggttg tttcaaaaga ggaaagagaa gtagcagttg aaacagtgtg tgtggacaaa
     3121 aacaaattgg atgatgcccg cgaatatgga attactccag gcaagctcac gcttattgaa
     3181 aagcttaagg atgcacttgg aaacgaggag tttgacataa acgattggac aaagaagtct
     3241 gtcaaggaaa ttgtaaatgc gactaatgaa gtcaaaaaga atgagaaaga aaagagaatc
     3301 aaaccaggcg atgatccaaa aaatgctgac gatcttgagg atgagaagga agaagtaaaa
     3361 gaggaaaaga aggatataaa agacgcaaaa aaagaagagg aaaagcaaga aaaagaagag
     3421 aaaaagaaac aggagaaaga agaaaaggaa agggaaaagc aggaaagaaa agaagagaaa
     3481 gagaaacaga agcaggaaaa gaaggaagaa aaagaaaagg aaaa
//
LOCUS       DAONVY010000217         3524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18438_length_3524_cov_3.402710, whole genome shotgun sequence.
ACCESSION   DAONVY010000217 DAONVY010000000
VERSION     DAONVY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18438_length_3524_cov_3.402710"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLI11_06500"
     CDS             complement(<1..527)
                     /locus_tag="PLI11_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52549.1"
                     /translation="MSKGKLVLLLCLVVSFALIAVACNDAQEPDPTKKPETTEAPSEG
                     TPTAEPTATPEPTETPEPTPTPIAGKLYEDAEILFEDNFDDNTYAAEFLSTFTGDLDI
                     FSNGMLKCYRPAELFNYSPMELDYDSYFQYEFWADISAWNETNESKRGTATWMGVMLG
                     VHVNNPSLNPTSDDG"
     gene            843..1553
                     /locus_tag="PLI11_06505"
     CDS             843..1553
                     /locus_tag="PLI11_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPZ52550.1"
                     /translation="MIGSFLNVCIYRIPNKESIVKGASHCTTCGEKITKRDLVPIISY
                     FVLRGRCRSCKAPFSIRYALVEFLTGILFVLCALKFKYTVETILMSLFLGALIVMTFI
                     DIDTMTIPDSIHIVILIIGIVLAFVSDMPLLERIIGFFSVSFILFLASVLSKGGIGGG
                     DIKLMAVSGFVLGYKLSVVGFFIGAVLASIFGIVISKGKRENLKRKIPLGPFLAVGMT
                     VSIFVGKYIVDWYLGLVL"
     gene            1685..2707
                     /locus_tag="PLI11_06510"
     CDS             1685..2707
                     /locus_tag="PLI11_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HPZ52551.1"
                     /translation="MKSVVLIPAYKPGEKFVVFLHELFKLNHKVVVVNDGSGEEYDRY
                     FETASRLGAVVLFYEENKGKGRALKTGLKYVLDNMPDVDFVVTADCDGQHTPQDIQKV
                     IDSGSANPNTIILGGRFSGKGDKVPRRSVLGNTVTRWVFRLATGLPIKDTQTGLRGLP
                     RFLIPKLVELKGERFEYEMNMLLYLKEWSVPFLEIPISTIYYDNNAGSHFNTFRDAWR
                     IFKQIVSFLLVAILSLIIDYSLFKVFSLLGIWIPLCYVFARIISSIFNFAMNARFVFK
                     NQDKKVVFKYYLVAFFIMIAGAGGTNLFASLGIPEILSKIMIDLPLFFASYILQREFV
                     FKKKMF"
     gene            complement(2704..3387)
                     /locus_tag="PLI11_06515"
     CDS             complement(2704..3387)
                     /locus_tag="PLI11_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52552.1"
                     /translation="MNHIFVGHYGSGKTEVSINFALNLKKENPDKDIAIIDLDIVNPY
                     YRTADAKEDLKKAGIHVEVPLFANTNVDVPALTAAMGALMENKDKLVVVDVGGDDLGA
                     KAVARYAEEINKGGYVMYFVLNPNRPFTSTLEQAVKMYDEIESSTTLKITGLVNNSNL
                     LQYTDKDVFLEGMEKVLELSRVKKVPVLLNTVFEKTADSLKEEDIKEGRVLVMKEHVS
                     LLWSREDDF"
BASE COUNT          937 a          652 c          762 g         1173 t
ORIGIN      
        1 taaccgtcat cggaagtggg gttcagtgat ggattgttaa cgtgcacacc aagcattaca
       61 cccatccatg tagctgttcc ccgcttggat tcgtttgttt cattccaagc tgatatgtct
      121 gcccagaatt catactggaa atatgaatcg tagtccagtt ccattggact gtagttgaat
      181 agttctgcag gacggtagca cttaagcatt ccgttggaga aaatatccaa atcgcctgta
      241 aatgtgctga ggaactctgc tgcatatgta ttgtcatcaa aattgtcctc aaacagtatt
      301 tctgcatcct cgtacaattt gcctgctatt ggtgttggcg ttggttctgg agtttctgta
      361 ggctcaggtg ttgcagttgg ttccgctgtt ggcgttcctt cagaaggcgc ttcagttgtt
      421 tcaggttttt tggttggatc cggctcttgt gcatcattgc atgctactgc tattagagca
      481 aaagatacta ccaaacaaag tagtaataca agttttcctt tcgacatgtg aacatccctt
      541 tcattagtat aatctaatat atttttacta aaatatagta tttttaacat taaaacagca
      601 gaatgtcaac aattatttta aataatgcag tgtgatatct gctatttttt ttagggatgt
      661 atcgctgaac gatgaacagc ctctttcttt ctcatatttt tttgcgttgg taaaatcaac
      721 acagtggtgt ataatacact tgttattaat caatttgttg gaggagatgc acactggacc
      781 tatctttcat gccttttgct tttcatgcaa tttgccttgc ctattggttt gtcgtgggtg
      841 ctgtgattgg cagcttcctg aatgtatgta tttacaggat tcccaataag gaatccattg
      901 tcaaaggagc ttctcattgc accacttgtg gggaaaagat aacgaagagg gatcttgttc
      961 ccataatcag ctattttgtt ctacgaggaa ggtgcagatc ctgcaaggct cctttttcca
     1021 tacgctatgc tttggttgag ttccttacag gaattctgtt tgtattgtgc gcgttgaagt
     1081 tcaaatacac tgtcgagacg attctaatgt ccctgtttct tggagctctc attgtcatga
     1141 catttataga tatcgataca atgacaatac cggattccat acatattgtc atcttgatta
     1201 ttggaattgt gctggccttt gtatccgaca tgccgctttt ggagagaatt attgggttct
     1261 tttcagtcag cttcatactt tttttggcct cagttctttc caaaggaggt attggaggag
     1321 gagatataaa gcttatggcc gtttcgggat ttgtgctggg atacaagctt agtgttgttg
     1381 gattttttat tggtgcggtg ctggcttcga tttttggcat tgttatttca aaaggaaaaa
     1441 gagagaacct aaagagaaag attcctttgg gtccttttct ggctgtagga atgactgttt
     1501 caatatttgt gggaaagtac attgtagact ggtatctggg gcttgtattg taagaaaggg
     1561 ttagaaaagc ttcacatttg tgaaagagtc tggtaatttt cttttaaatg aaatataatg
     1621 taggtgtgtc tcatcttcta ctaaacgtgg cagagagctc aataagaaaa aggtgagctt
     1681 ttagatgaaa agtgtagttt taattccagc ttacaaacct ggtgaaaagt ttgttgtgtt
     1741 tttgcatgaa cttttcaaat tgaatcacaa agttgtagta gtaaatgacg gaagtggaga
     1801 agaatacgac aggtattttg aaacagcttc caggttggga gctgttgttt tattttatga
     1861 ggaaaacaag ggaaaaggaa gagccttgaa aacagggttg aagtatgttt tagacaacat
     1921 gcccgatgtg gattttgttg taacagctga ctgcgatgga cagcatactc cgcaagatat
     1981 tcaaaaggtt atagattccg gtagtgcaaa tccaaacacc atcatacttg gaggaaggtt
     2041 tagcggaaaa ggggacaagg ttcccagaag atccgtgctg ggaaatactg taaccaggtg
     2101 ggttttcaga cttgcaaccg gtcttcccat aaaggatacg cagacaggat taagaggcct
     2161 gcccaggttc ctaatcccaa aacttgtgga actgaagggg gagaggtttg agtacgagat
     2221 gaacatgctt ctttacctca aggagtggtc cgttccattt cttgaaatac ccatcagcac
     2281 gatatattat gacaataatg cagggtcgca tttcaacacc ttcagggatg catggaggat
     2341 attcaagcag atagtgagtt tccttcttgt ggcaatattg tcgcttatta tagactattc
     2401 gcttttcaaa gtgttttcct tgttaggaat atggattcct ttatgctatg tttttgcaag
     2461 gattatatca tccatattca actttgcaat gaatgccagg tttgttttca agaaccagga
     2521 caagaaagtc gttttcaaat actatcttgt tgcgtttttt atcatgattg cgggcgcagg
     2581 tggaacaaac ctctttgcaa gcctgggcat tccagaaatt ctgtcaaaga taatgatcga
     2641 tcttccgttg ttttttgcaa gctacatact gcaaagagag tttgttttca aaaagaagat
     2701 gttttagaaa tcatcctctc ttgaccaaag aaggctgacg tgttctttca tcacaagaac
     2761 cctgccttct ttaatatctt cttctttaag ggaatcggct gttttttcaa agaccgtatt
     2821 caacaacacg ggtacctttt tcacgcggga cagttccaga accttttcca ttccttcaag
     2881 gaacacatct ttgtccgtat actgcaaaag atttgagttg ttcaccagcc ccgttatttt
     2941 cagggtggtg cttgattcta tctcatcata catttttaca gcctgttcca aagtgcttgt
     3001 aaagggacgg ttgggattta ggacaaagta cataacatag ccgcccttgt ttatctcttc
     3061 ggcatatctt gccactgcct ttgcacccag gtcatccccg cccacatcca ctacaacgag
     3121 tttgtccttg ttttccatca gggcgcccat ggctgcggtg agggcaggca cgtccacgtt
     3181 ggtgtttgca aacaaaggta cttccacatg aatgcccgcc tttttgagat cttcctttgc
     3241 atcggcggtt ctgtagtatg gattgactat atccaggtct ataattgcaa tatccttgtc
     3301 aggattctcc tttttcaaat ttagtgcaaa atttatcgat acttccgttt ttccgcttcc
     3361 ataatgtcct acgaatatgt gattcatatc tttcacctta aatattttta tttgatttaa
     3421 cataagatat tgtataactt tatgtgataa aatacaaata attgaaaaga cagggggctc
     3481 aaggtcatgt agcactttac tggttatggc taaaagagta taat
//
LOCUS       DAONVY010000218         3524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18439_length_3524_cov_2.859325, whole genome shotgun sequence.
ACCESSION   DAONVY010000218 DAONVY010000000
VERSION     DAONVY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18439_length_3524_cov_2.859325"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLI11_06520"
     CDS             complement(<1..435)
                     /locus_tag="PLI11_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPZ52553.1"
                     /translation="MSKKIWLSSPHMSDEGYELQFIHEAFESNWIAPLGENVNKFEQE
                     FAAKVGAKHAAALASGTAAMHLALKAVGVGPGDIVLCPTLTFIATVNPVIYQNAIPVF
                     IDSNYETWNMDPNSLEDAFKKYGDRVKAVIVVHLYGLSADMDN"
     gene            complement(704..1399)
                     /locus_tag="PLI11_06525"
     CDS             complement(704..1399)
                     /locus_tag="PLI11_06525"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HPZ52554.1"
                     /translation="MALGRKVNEHSDAPYKYKLDKTSSFMMQESYKAIRTNLQFAMAG
                     NGKKAFIISSSFPAEGKSITCSNIAITLAQTANRVLVIDADLRKPTQHKIFRVDNSHG
                     LSTILVGFDKLGEAIHKDVDKNLDLITSGPIPPNPSELLGSNRMDVLLDKLNEFYDYI
                     VIDTPPINVVTDAVVLAPKTAGVALVSRQKVTTYDEIKKALDAIEFAKVKVLGIIVND
                     VNVKRNNKYYDYE"
     gene            complement(1386..2117)
                     /locus_tag="PLI11_06530"
     CDS             complement(1386..2117)
                     /locus_tag="PLI11_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPZ52555.1"
                     /translation="MDYENGGKFMQDTIDIRYIINLLISKLVWIIAAMLTGILIMFYV
                     ASFVITPKYTSSIRLFVDVEDGSSLTKTQLDLSRSLVSTYIVLLESRDSLKVIASDLN
                     TAALSAEDVEKYINMRAVNNTEVLEIEAVTPDPVLSAEICNSFAKNAPTIIENIVGNS
                     RVSVIDTAVVPTKPSSPNVPMYTALGGLLGLILSCGVILLIDHLDNTVKGGEDLSQRY
                     GLPVLGEVPSFHTKDKGGYYSYGSR"
     gene            complement(2207..>3524)
                     /locus_tag="PLI11_06535"
     CDS             complement(2207..>3524)
                     /locus_tag="PLI11_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52556.1"
                     /translation="FDEKGYYSPKSEDGTNKGANGAIIEDLNLEVNRRFLYLYDFGRN
                     KLFEIIVKAKEPASRPHPPELLASVGVLDDFGKEYNPDEGLRFLFHHKKHADSFRVEK
                     DIYCYHHNLLHISTFHFDILTGVLKDVLDPVYLLADDYEHYADSLQANMHRVFQILTP
                     DALEILRWLYTEPVYDVVESITSRNLNALSAIAGFGFVDVGIEPGKGRDRLIFYLPKD
                     SDHFAPYFYTDEYKEILKRTKMLDRAVKAIVELYGVVDLELLKTQLRELFDIDESMVE
                     VVMHTIYPRVLEETYFMVNEFGTDYISKYMGDDLFSVLALRRGIDPKDYRKFTRKEAR
                     LVAQEGITGILPGYDELVHQLYSNTILTLDGVKLIAENIVRLRCGGYDNRSIIEYVER
                     KLSCKWDNLTPIIRDLITNILDECPVHAFGGHRYKDFMDKNNGLSE"
BASE COUNT          997 a          805 c          605 g         1117 t
ORIGIN      
        1 attgtccata tcagcagaaa gaccatacaa atgcactacg atgacagctt taaccctgtc
       61 accatatttc ttaaaggcat cctctaggga attgggatcc atgttccatg tttcatagtt
      121 actgtctatg aaaacaggaa ttgcattttg atatattaca ggattaactg tggcaatgaa
      181 ggtaagtgtt ggacagagaa caatatcccc cggtcctaca ccaacggcct ttaaagccaa
      241 atgcattgca gcagtacctg atgccaaggc agcagcatgt tttgccccta ccttcgcagc
      301 aaactcctgc tcaaacttgt ttacattctc cccaagaggg gcaatccaat ttgattcaaa
      361 tgcttcatgt ataaattgta attcataacc ttcatcactc atatgtggtg atgacaacca
      421 aatctttttt gacattttct ctccctactc aatactcttc gacagaatca aagctcttct
      481 cgataacata gtaaataaat ggaaatttaa ataagtgcac gctagggact ttgagcttgt
      541 ggctcaattt ttgagtgcaa atataacgac aaaccataag ctaacacgcc tagcgatgat
      601 tagattacag tatgcctttt tccatttatt ttaaaaagca ctagctgtag ttctatccct
      661 caactaatgc tacatctact tgctatatat ctttcttttt tttctattcg taatcgtagt
      721 acttgttgtt cctctttaca ttaacatcat ttactatgat tccgagtact ttcaccttcg
      781 caaactcaat tgcatccaat gccttcttaa tttcgtcata cgtagtaacc ttctgccttg
      841 agacgagggc aactcctgca gtcttgggtg ccaaaaccac cgcatccgtc accacattta
      901 taggaggcgt gtcaatcact atgtagtcgt aaaattcatt aagcttgtcc aacaacacat
      961 ccattctgtt gcttcctagc aattctgatg gatttggagg aattggacct gatgtgataa
     1021 gatcaaggtt tttatccacg tctttgtgta ttgcctcgcc tagcttgtca aatccaacca
     1081 atattgtaga cagtccatgt gaattgtcaa ccctgaatat cttatgctga gttggcttgc
     1141 gtaaatctgc atcaattaca agtaccctat tcgcagtttg tgccagagta atggcaatat
     1201 tggaacaagt tatactcttt ccttctgcag gaaaggagga tgaaattatg aatgccttct
     1261 tcccattgcc tgccatggca aactgcaagt ttgtccttat tgccttgtaa gactcctgca
     1321 tcataaagga agatgtcttg tctaacttgt acttgtatgg tgcgtcactg tgctcgttta
     1381 ctttcctacc tagagccata actgtaatat cctcctttat ctttggtatg aaaacttggt
     1441 acttctccta atacaggtag tccatatctt tgggacaagt cctctccacc cttgacggta
     1501 ttgtcaagat ggtcaatcaa gaggataacg ccacatgata gtattaatcc caacaatcca
     1561 cccaaagctg tgtacattgg tacattgggt gatgatggtt ttgttggtac aactgccgtg
     1621 tcaataaccg acacgcggct gttcccaaca atgttctcga tgattgttgg tgcattcttt
     1681 gcaaaggagt tacagatttc agctgacaat acagggtctg gggttacagc ttcaatctcc
     1741 agaacttccg tattgttaac tgcacgcata tttatatact tctctacatc ttctgcgctt
     1801 aaagcagctg tattcaaatc agatgcaatc acctttaatg aatccctgct ttcaagcagc
     1861 acaatgtatg ttgatacaag gcttctactt aaatccaatt gggtttttgt cagtgacgac
     1921 ccgtcttcca catcaacaaa tagtctaata cttgatgtgt acttgggagt tatcacaaaa
     1981 cttgccacat aaaacatgat tagaattcca gtcagcattg cagctataat ccaaaccagt
     2041 ttacttatca acaagttgat gatatagcga atgtctatcg tatcctgcat gaattttcca
     2101 ccattttcat aatccatagt attatctttc gtctcctaaa attctcagag ccctgtacca
     2161 tgctcacttt gctattatat ttccaacatt ttctgttgtc aatgttttat tcggacaaac
     2221 cattattttt atccataaaa tccttatacc tgtgtccgcc aaaagcatga acaggacact
     2281 catctaagat attcgttatc aagtccctta taatcggggt aaggttatcc catttacatg
     2341 ataattttct ctccacatac tcaattatgc ttcgattgtc ataaccgccg catcttagcc
     2401 ttacaatatt ctcagcaatg agttttacac catccaaagt aagaatagtg tttgagtaaa
     2461 gctgatgtac aagctcatca tatccaggca aaatacctgt gattccttct tgtgcaacaa
     2521 gccttgcttc tttccttgtg aactttctgt aatcttttgg atcaatccct cttctcaatg
     2581 ccagcactga aaacaggtca tcacccatat actttgatat ataatctgtg ccaaactcgt
     2641 taaccatgaa ataggtttct tcaagaaccc ttgggtaaat ggtgtgcatt acaacctcta
     2701 ccatgctctc atcaatatca aacaattccc taagctgtgt tttaagaagc tcaagatcca
     2761 caactccata tagttccaca atcgccttta cagccctgtc gagcattttg gttcttttaa
     2821 gaatctcctt atactcatcg gtatagaaat aaggggcaaa gtggtcagag tcctttggca
     2881 agtagaatat cagcctgtcc cttccttttc caggctcgat tcccacatcc acaaaaccaa
     2941 aacctgctat tgcagaaagc gcattaaggt tgcggcttgt tatgctttca accacatcat
     3001 ataccggttc tgtatacagc catctcagga tttccaaggc atccggtgta aggatttgga
     3061 acaccctgtg catgttggcc tgtagactat cagcataatg ctcatagtca tcggccaaaa
     3121 ggtacacagg gtccaggaca tccttcaaaa ctcctgtaag gatgtcaaag tgaaatgtgg
     3181 atatgtggag aagattgtga tgataacaat atatatcctt ttcaactctg aaagagtcag
     3241 catgcttttt gtgatgaaag agaaatctca aaccttcgtc aggattgtac tctttgccaa
     3301 aatcatcaag tacacctacc gatgccaaaa gctcaggagg gtgtggcctg gaggcaggtt
     3361 cttttgcctt tactataatc tcaaataatt tgtttcttcc aaaatcatag aggtacaaaa
     3421 acctcctgtt aacctcaagg ttaaggtcct caataatggc accgtttgcg cctttgttgg
     3481 tgccatcttc tgatttggga ctgtaatagc ctttttcatc aaac
//
LOCUS       DAONVY010000219         3521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18460_length_3521_cov_3.351414, whole genome shotgun sequence.
ACCESSION   DAONVY010000219 DAONVY010000000
VERSION     DAONVY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18460_length_3521_cov_3.351414"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="PLI11_06540"
     CDS             complement(<1..2260)
                     /locus_tag="PLI11_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPZ52557.1"
                     /translation="MAGFFGKRIKDEELMENINVGQRLSKTGFFTHDLVKDKLIMTSQ
                     VYNILCMESHDNIYNVIHDEGQKAFFEHEEMSYEMTLEGQEKHLQVWTRTVFDKEKRP
                     VKIYGALQDITDRKLIENNLKAIGEDLSIAQKVSGVGSWRYDVLKDEFYGTEEMFSIY
                     GIKPEEFDNDFESTIKLIHPDDRDNVRHCLAEHFAGRSCEIEFRIPQKDGSVKYVKGK
                     GEAILNELGQTVSVIGTLRDITQEKLLQMQLEQSNRMMEQGQKLAKTGSWVFDIATNK
                     STSSKGVYRIFGIDPENFDNTYESYVSLVHYEDRDRVDKLLGARLKGPFDMKFRIVRP
                     DGEVRFIHQVSEYVFDENENPVYLYGVIRDITKEKKLNEELEKQQEEIAGLKNRFQML
                     LQESSDVYEIIKPDGTIVCISEAVEEVLGSKPEDRLGKNVYDFYEGEELEKVRNFINS
                     VVENPGVRLKGDVKLRLKDGKELNIEAELQNLMDNPFIEGIVIHFRDISDRVEMKNKL
                     TYISTHDDLTKLPNQSYFKEQLKSYCSEVKDTKMPFALMFISISGLSYVIYTLGYSIE
                     QLLVLEIVKRLKELFNDNFIARYSEDDFAVVLKSQEEDYESLAKRIINTLTKPYKIQQ
                     YELTVSVNVGINFFADCNKDVDSFVQDTLQTVLRAKREGKNRYRFYSSSFDIQNFKDF
                     FFRNDLRKAIETDQLSVRYQPIVNIWSGEILFVEALVRWEHPEWGLISPGEFIAIAEE
                     MGLIIDVGNWMLN"
     gene            complement(2266..2889)
                     /locus_tag="PLI11_06545"
     CDS             complement(2266..2889)
                     /locus_tag="PLI11_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPZ52558.1"
                     /translation="MKHEWRKHEKELYIPKEKPELVDVPPMNFITISGKGDPNQQDFA
                     DRIGALYPIAYAIRMMPRNGFTPEGYFEYTVYPLEGVWDLTEEGRKKETLVKDDLVYT
                     IMIRQPDFVTREVFERAVEIAKKKKNNPLFDEVRFETIKDGLSVQMMHVGPFDEEYKT
                     FEKMKEYISENNLKLRTLVHREIYISDIRKTEQEKLKTVLRYLVENR"
     gene            3094..>3521
                     /locus_tag="PLI11_06550"
     CDS             3094..>3521
                     /locus_tag="PLI11_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52559.1"
                     /translation="MFTILENDITIIGPVTIICALIAYFLYKKHRDSKMVFFHILFCF
                     GVYAWFAHNFLPMPLSSDMWKMELRPLNRSKLECLELIPFRAIYSDISRMGFNELVRS
                     ALFNVYVSFAPIPMLTGFSIKNAYANITRTKAIISSIVLG"
BASE COUNT          948 a          862 c          539 g         1172 t
ORIGIN      
        1 tgttcagcat ccagtttccg acgtcaataa taagtcccat ctcttctgca atagcaataa
       61 attctcctgg agatatcagt ccccattcgg gatgctccca tcttaccaag gcctctacaa
      121 acagtatttc accgctccaa atattcacaa tgggctggta tcggacactc aactgatccg
      181 tttcaattgc ctttctcaaa tcgtttctaa aaaagaaatc tttgaagttc tgtatgtcaa
      241 aactggacga atagaaccta tatcgattct ttccttccct ctttgctctt aggactgtct
      301 gcagcgtatc ctgcacaaag gaatccacat ccttgttgca gtcagcaaaa aagttgattc
      361 ccacgtttac ggatacagtg agctcgtact gctgtatctt gtatggtttt gtaagggtgt
      421 taataatcct ttttgcaagg gattcgtaat cctcttcctg acttttgagt acaacggcaa
      481 agtcgtcctc ggaatagcgg gcaataaaat tatcattaaa cagttccttt aatcgcttta
      541 caatctccaa aacaagcagc tgttcaatac tgtatcccaa tgtgtatatt acataactca
      601 gaccgcttat ggatataaac attagggcaa aaggcatttt tgtgtccttt acctcactac
      661 agtatgattt aagctgctct ttgaaatagg attggtttgg aagcttggta aggtcatcgt
      721 gtgttgagat atatgtgagc ttgttcttca tctccactct gtcgcttatg tccctgaagt
      781 ggatgacaat accttcaatg aaaggattgt ccatcaaatt ctgaagttca gcctcaatat
      841 ttaattcctt tccatccttt aatctcagct tcacatcacc ctttagcctg accccaggat
      901 tttcaaccac actgtttata aagttcctaa ccttctcgag ctcctctcct tcataaaagt
      961 catatacatt ttttccaaga cggtcctcag gcttgcttcc cagtacttcc tccaccgctt
     1021 cgctgatgca tacaatagtg ccatccggtt tgattatctc atacacatcg ctggattcct
     1081 gcagaagcat ctggaacctg ttcttcaatc cagcaatttc ctcctgttgc ttttcaagct
     1141 cttcgttaag ctttttctcc ttagttatgt cccgtattac cccatacaaa tatacaggat
     1201 tttcattttc atcaaaaacg tactcggaca cctgatggat aaaccgcacc tctccgtcag
     1261 gccttacaat cctgaacttc atatcaaaag gccctttcaa tcttgcgcca agaagcttgt
     1321 caacccggtc cctatcctca taatgcacca atgacacata actctcatat gtattatcaa
     1381 aattctcagg atctattcca aatattctgt aaactccctt agaactggta cttttgttgg
     1441 ttgcaatatc aaacacccag ctgcctgttt tggcaagttt ctgaccctgt tccatcatcc
     1501 tgttactctg ctccagctgc atttgaagaa gcttttcctg ggtaatatcc ctcaatgtgc
     1561 caataacgct tacagtctgc ccaagctcat taagaatggc ttctcccttt ccttttacat
     1621 acttcacaga gccgtctttt tgaggaatgc ggaactctat ttcgcaggac ctgcctgcaa
     1681 agtgttcagc tagacagtgt cttacattgt ctctgtcgtc agggtgaata agttttattg
     1741 tgctctcaaa gtcattgtca aactcttcag gctttattcc gtatatgctg aacatctctt
     1801 cagttccata gaactcgtcc tttaatacat catacctcca acttcccacg ccggatacct
     1861 tctgcgcaat acttaagtct tctccaattg ccttaaggtt gttctcaata agttttctat
     1921 cagttatatc ctgcaatgct ccatatatct ttactggtct tttttcctta tcaaaaaccg
     1981 tccttgtcca tacctgcaaa tgcttctcct gaccttccag cgtcatttca taactcatct
     2041 cttcatgttc aaaaaaagcc ttctggcctt catcatggat aacattgtag atattatcat
     2101 gtgattccat gcaaagaatg ttataaacct ggctggtcat aatcagcttg tccttaacca
     2161 aatcatgcgt gaagaaacct gtcttcgaca atctttgtcc aacattgata ttttccatta
     2221 gctcttcatc ttttattctc tttccaaaaa aaccggccat gctcctcacc tgttttcaac
     2281 aagatatctc aatacagtct tcaacttctc ctgctccgtt ttcctaatat ccgatatata
     2341 tatttccctg tgtacaagag tcctaagttt aagattattc tcagaaatat attccttcat
     2401 tttctcaaac gttttgtatt cctcgtcaaa gggacccaca tgcatcatct gtactgacaa
     2461 accatccttt attgtctcaa acctgacctc atcaaataat ggattgttct tcttcttttt
     2521 tgcaatctca actgctcttt caaagacttc ccttgtaaca aaatccggtt gccttatcat
     2581 tattgtgtaa accaaatcat cctttacaag ggtctccttt tttcttccct cctcagtcaa
     2641 atcccagacc ccttccagtg gatatacggt atactcaaaa tacccttcgg gggtaaatcc
     2701 attccttggc atcatcctaa ttgcatacgc tatcggatac aatgcgccta tcctgtctgc
     2761 aaaatcctgc tggttgggat ctccctttcc acttattgta atgaagttca tggggggcac
     2821 atcaacaagc tcaggttttt ccttcggaat gtataactcc ttttcgtgct tcctccattc
     2881 gtgtttcatt ttaaaactcc caattttaat aatatatata attttaccaa gaaatcccct
     2941 gtaggaaaag agtaaaactt tttaacaatt caggtaaata ccacaattca tgtaaattta
     3001 accataatgt tatactttac acacaatttc ttgattaaat aattgacaaa caatgctgca
     3061 agaattgaat aaagtcaaaa ggaggatgct ctcatgttta caattctgga aaatgacata
     3121 accatcatag ggcctgtgac cataatctgc gctttaattg catactttct ttacaaaaaa
     3181 caccgggatt caaaaatggt cttctttcat attctattct gttttggagt ctatgcctgg
     3241 tttgcgcata atttccttcc catgccgctg tctagtgaca tgtggaaaat ggaactacgt
     3301 cctctaaaca gaagcaaact agagtgtttg gagctgatcc cattccgtgc aatatattca
     3361 gatatatcca gaatggggtt caacgaattg gtaagaagtg cgcttttcaa cgtatatgtc
     3421 agttttgctc caattcccat gttgactggt ttttcaataa agaatgcata tgcaaatatt
     3481 acaaggacga aagcaatcat ctcctccatt gtcctgggct t
//
LOCUS       DAONVY010000220         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18483_length_3518_cov_3.548657, whole genome shotgun sequence.
ACCESSION   DAONVY010000220 DAONVY010000000
VERSION     DAONVY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18483_length_3518_cov_3.548657"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLI11_06555"
     CDS             complement(<1..927)
                     /locus_tag="PLI11_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016937258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPZ52560.1"
                     /translation="MTDFAKTKTKAITIVLIIVLSLISLSIGAKSFTWIGVLKGEANH
                     LQTLFISRLPRLLSILITGAGLSISGLLMQTITGNKFVSPTTAGTMDWSRLGVLIAIL
                     LAGEQSKLLRILVAFIIAFLGNLIFISITQRVQYKSAVMVPLIGLMLGSVVSSLTTFI
                     SYRYNIIQNVSSWLQGNFSLVIKGRYELLYIGMPMVVIAYLFAGRFTIAGMGESIATN
                     LGLDYKKTVLLGLLIVSFLTTTIVITIGSIAFVGLIIPNIVSLYKGDNLRNCLFDTAA
                     LGALFLLVCDIVGRLVIYPYEVSISVVVSVLGS"
     gene            1141..1821
                     /locus_tag="PLI11_06560"
     CDS             1141..1821
                     /locus_tag="PLI11_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPZ52561.1"
                     /translation="MDKQKLLLVEDDETIALGLSYALEKQFDVKVCRNVMTALEEIKG
                     IEYDIALIDITLPDGAGYEVCKAIKEKWNTGIIFLTARDDEASVVMGLDIGADDYVTK
                     PFRLRELMSRINSVLRRRGVKDTVDNIKKYGNVVVDLNRGAVYKNGKEVFLTAQEYKL
                     LLKLITNEGMVMTRNNLLESIWDISGDFVNDNTLTVYIKRLREKIEDDPQNPQIIKTV
                     RGRGYIIE"
     gene            1818..2822
                     /locus_tag="PLI11_06565"
     CDS             1818..2822
                     /locus_tag="PLI11_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPZ52562.1"
                     /translation="MMLRNPEIKKFMIIQLLVCLVFVLISSLMNAVAGWMVLIVTIIL
                     NALFFGFTFLRYRKIKELVMYINRISNGEFRLDVRDNKEGELSILKNEIYKVTEKLTE
                     YNQRLQQEKLYLSQSLADISHQLKTPLTSMIIRCEILNDPDLPQDKRKEFVSQIIVQL
                     ERIDWLVSTMLKMSRLDAGVVTMKKMDIYVSDLVEKAKSALIAPMESKEIELVTQFAS
                     EDKLKCDFNWTCEAVINILKNCIEHTPQKGRIEIDYSDNPLYHELTIKDNGAGISKKD
                     LPHIFTRFYRGENASAESTGIGLAMAKSIINAQHGNIIVESQKNKGCKFMIRLYKTVV
                     "
     gene            2907..>3518
                     /locus_tag="PLI11_06570"
     CDS             2907..>3518
                     /locus_tag="PLI11_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52563.1"
                     /translation="MEILKVENVTKIYGKGNTAVKALDKVSLSVEKGEFIAVVGPSGS
                     GKSTLLHIIGGVDNPTSGKVYINNTDVFSLNENNLAIFRRRNIGLVYQFYNLIPVLNV
                     EENILLPLLLDGRKEDRNHLNNLLSTLNLSDRTRHLPNQLSGGQQQRVSIGRALINNP
                     ALVLADEPTGNLDSKNSAEIIELLQYYNKTYNQTLIIITHDERV"
BASE COUNT         1162 a          643 c          751 g          962 t
ORIGIN      
        1 gctacccaaa acactgacta ccacgcttat agacacctca tagggatata tgacaagtcg
       61 cccaacaatg tcgcatacaa gtaagaacaa tgctcccaag gctgcggtgt caaaaagaca
      121 gtttctcaag ttgtcacctt tatatagcga aactatgttg gggattatca ggccgacgaa
      181 agcaatgctt ccgatggtta tcacaatggt tgtagtgagg aatgagacta ttaaaagacc
      241 caacagtacg gtttttttgt aatccagtcc aaggtttgtt gcaatacttt cacccatgcc
      301 agctatggta aatctacctg cgaacagata tgctattacc accattggca ttcctatata
      361 aagaagttca taccgacctt taataaccag tgagaaattt ccctgaagcc acgaggaaac
      421 attctggatt atgttgtacc tgtaggaaat gaaagtggta agagaactta cgacacttcc
      481 aagcattagg cctatcagag gcaccataac tgcactttta tactgcactc tctgagttat
      541 acttataaaa attagattgc ccaaaaaagc tataataaaa gctaccagaa ttcttagcaa
      601 ttttgactgc tcacctgcaa gaaggattgc tatcaggact ccaagcctgg accagtccat
      661 ggttccggct gttgttggtg atacaaactt gttgccggtt atggtctgca tgagcaggcc
      721 tgaaatgctg agtcctgcgc ctgttatcaa tatgctcaat agtcgaggca atctgctaat
      781 aaacaaggtt tgcaggtgat tggcttctcc tttgagaaca ccaatccacg taaagctttt
      841 tgcaccgatg cttagagata tgagggataa aactataatt aaaactattg ttattgcttt
      901 ggttttggtt tttgcaaagt ctgtcacgct atcatctcca tattctagtt agcatatgct
      961 aactgatttg gaatattacc atgtgcttta tatgctgtca atgcaattcc ggaaatttaa
     1021 taattgtaaa gagttagaca catctaactt ttagtcttca ttttgctcag accaaattga
     1081 ttattcacgg caacaatagt ataatcttat cgcttatcat gttggcaggc aggtgagacc
     1141 atggacaaac agaagttgtt gctagttgaa gatgatgaaa ccattgcact gggtctttca
     1201 tacgctcttg aaaaacagtt tgatgtaaaa gtatgcagaa atgtaatgac ggcactggag
     1261 gaaataaagg gcattgaata tgacattgca ctaattgaca ttactttacc tgatggtgca
     1321 ggttatgagg tctgcaaggc tataaaggaa aagtggaata ccggaattat ttttcttact
     1381 gcaagagacg atgaagccag tgtggtgatg gggcttgaca ttggtgcgga tgattatgta
     1441 accaagccct ttcgtctaag ggagctcatg tcccgcatca acagtgtgct gaggagacgc
     1501 ggagtaaagg atactgttga caatattaaa aagtatggca atgttgttgt ggacctgaat
     1561 cgcggtgcag tttacaaaaa tggaaaagag gtttttctca ctgcacagga gtacaagctt
     1621 cttttgaaac tcataaccaa tgaaggcatg gtaatgacca gaaataatct gcttgagagc
     1681 atatgggata tctcgggaga ctttgtcaat gataatacat tgacagtgta tataaaaagg
     1741 ctgagagaaa aaatagagga cgatcctcag aatcctcaaa taatcaaaac ggtccgagga
     1801 agagggtata ttatagaatg atgctgagaa atcccgagat taagaagttt atgatcatcc
     1861 aattacttgt ctgcctggtt tttgtattaa tctcttcact catgaatgct gtagctggat
     1921 ggatggttct tatagttaca attatcctta atgccctgtt ttttggattt acatttctca
     1981 gataccgcaa aataaaagag ctggtaatgt atattaaccg aattagcaat ggtgagttta
     2041 gattggatgt aagggacaat aaggaaggcg agctgagcat cctgaaaaac gagatttaca
     2101 aggttaccga aaaacttact gagtacaacc aaagattgca acaagaaaaa ttgtatttgt
     2161 cccagtcgct ggcagatata tctcatcaat tgaaaacacc tcttacatcg atgattatta
     2221 ggtgtgagat actaaatgac ccggacctgc ctcaagataa aagaaaagag tttgtttcac
     2281 agataattgt tcagttggaa agaatcgact ggcttgtatc aaccatgctg aaaatgtccc
     2341 gattggatgc aggggttgtt acaatgaaga aaatggacat ttatgtgtct gatttagttg
     2401 agaaagcaaa atctgcactt attgcaccca tggagtcgaa agagattgaa ttggtcacgc
     2461 agtttgcttc cgaggataaa ttaaaatgtg attttaactg gacttgtgaa gcggtaataa
     2521 acatattaaa gaactgtatt gagcacactc cgcaaaaggg aaggattgaa atagattact
     2581 cggataatcc tttgtaccat gagctgacaa tcaaggataa cggagcggga atatccaaaa
     2641 aggatttgcc gcatatcttt accaggtttt accgtggaga aaatgcttct gctgaaagca
     2701 caggcatcgg tcttgcaatg gcaaaaagca taataaacgc gcagcacgga aacataatag
     2761 tagaaagcca aaagaacaag gggtgcaaat tcatgatacg tctgtataaa accgttgtgt
     2821 gacaaaattg taataattgc tgtcattcaa ctgtcactta agtgcaataa aatgtaccca
     2881 gcaaataaat caaaaagagg tgcattatgg agattttaaa agttgaaaat gttacaaaga
     2941 tatatggtaa agggaacaca gcggtaaagg ctcttgacaa agtctccttg tcagttgaaa
     3001 agggagagtt tattgctgtg gttggccctt caggatcagg aaaatccacg cttcttcata
     3061 taattggagg tgtggataat cccacatcag gcaaagtgta catcaacaac acagatgtat
     3121 ttagcctaaa tgaaaacaat ctggcgatat ttagaagaag aaacatagga cttgtatatc
     3181 agttctacaa cctgatacca gttctaaatg tagaagagaa catactactg ccccttctgc
     3241 ttgacggaag gaaggaagac agaaaccatt tgaacaattt gctcagtaca cttaacttat
     3301 ccgaccgtac cagacacctg ccaaaccagt tgtccggtgg ccaacagcaa agggtgtcca
     3361 taggtcgggc gctgataaac aacccggcac ttgtcctggc cgacgaacca acaggaaacc
     3421 ttgacagcaa aaattcagct gaaataattg aacttttgca atactacaat aaaacatata
     3481 atcaaaccct gattataatt actcacgatg agcgtgtt
//
LOCUS       DAONVY010000221         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18544_length_3510_cov_2.867438, whole genome shotgun sequence.
ACCESSION   DAONVY010000221 DAONVY010000000
VERSION     DAONVY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18544_length_3510_cov_2.867438"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLI11_06575"
     CDS             complement(<1..788)
                     /locus_tag="PLI11_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52564.1"
                     /translation="MSEKLKRVLLLVLFISLLLGNYDLRTIQSNDNTFYMTDNKSYNA
                     EDTVYKLDFEESSLTSYKSEPGWKFTNEGNGVIENGKLKCKSGKSFDIRSEFKLGDEY
                     GVTNAPIEFEMLMTKGTVSVGTKLISTVSVVDTGIWFHISPNDVKVTIGGTEAVEKIT
                     HGMSLDVERKYRITSSGSRTRLYISEGDDFIKVAEVYLDADNIFSISDKDGNLVGEKV
                     ENSRTPYTGLWKMTIDRLDGHIDNLTYTNTVVTQVFPDTEQRPV"
     gene            1005..2042
                     /locus_tag="PLI11_06580"
     CDS             1005..2042
                     /locus_tag="PLI11_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ52565.1"
                     /translation="MYKPGELLERLIPLDEDELIYRELFFAKQNKDTYEEFLRKHEDF
                     RNNELLLVENTTAFSFCLKEENILLFDNLESNICVLKHKRYSPMRGHSHTFFEMIYVL
                     KGSCDNIIEGTPIRLNEGDICIMSSDVTHAMSVFDDSLIINILIKKTTFKETFFKLLK
                     DDNILSTFFTRILYTNNASNYILFHTKGDIRLHTLLEYLIWDGIFEGHKNNSDLREYL
                     LLASFHYMLENHVDNIELPKTVDTNIPNIVQILRYIKENYKTIKLGDLARHFNYAPNY
                     ISNLIKSTTGYTFSEILIETKLRNACKFLRDTSLPVHEIAHMVGYDSNEHFHRVFKKY
                     TGYTPNEYRTM"
     gene            2060..>3510
                     /locus_tag="PLI11_06585"
     CDS             2060..>3510
                     /locus_tag="PLI11_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPZ52566.1"
                     /translation="MNLPRPEYPRPIMRRDNWINLNGEWEFELDPGQSGRERNMHANG
                     VFSKKIIVPFCPESKLSGLEIKDFMPCVWYRRTFELKELKRTLIHFGAVDYHAEVWIN
                     GHSVGTHVGGYVGFTFDITDFVKVGVNEVVVCAADDTRSGKQPIGKQSFRYDSHGCHY
                     TRTTGIWQTVWLEQVSDTYISNLKMVPDINNQRINITVFLEGNVRDGSNLKIDARSSY
                     EGKDTGSVEVKGNWRQVSFSIQLSELHLWEPLKGRLYDLEITLYENDSLVDTIKSYFG
                     MRNVSLTDKAILINGKPVFQRLILDQGFYMDGVYTAPSDDELRKDIERSIAMGFNGAR
                     LHEKVFEPRFLYWADKLGYLCWGEHANWGLDISNPEGMMAFLPEWVEILNRDISHPSI
                     VGWCPFNETQGNQDNRILEYIYKVTREIDPTRPIIDTSGWVHTKFTDVYDMHDYDQDI
                     ESFARKLDPFKTGDTPNVTLSDPATYIKGTPYFV"
BASE COUNT         1085 a          704 c          715 g         1006 t
ORIGIN      
        1 tcgacaggcc tctgctccgt atcagggaaa acctgtgtaa ccacggtatt ggtatacgta
       61 aggttgtcta tatgtccatc cagtctgtct atcgtcattt tccacagacc tgtatatgga
      121 gttcttgaat tttctacttt ttcgcctacg agattgccgt ccttatccga tatggagaat
      181 atgttgtcag catccagata tacttctgct actttgataa agtcatctcc ctcggagata
      241 tacaggcgtg ttctggaacc actgctggtt attctatact ttctctccac atccagagac
      301 attccgtggg ttattttctc tacagcttcg gttcccccaa ttgtcacctt tacatcattg
      361 ggcgaaatgt ggaaccaaat tccggtatca acaacagaaa ctgttgagat aagttttgtt
      421 ccaacactta ccgttccttt cgtcataagc atttcaaact ctatgggtgc gtttgtcact
      481 ccatactcat caccgagctt gaattcactt ctaatgtcaa agctcttgcc actcttgcac
      541 ttgagctttc cattctcaat tacgccgttg ccttcattgg taaacttcca gccaggctcc
      601 gacttgtagg aggtcagaga cgactcctcg aagtccagtt tgtatacagt atcttctgca
      661 ttataacttt tgttatccgt catgtagaat gtattatcgt ttgattgtat tgttctcaaa
      721 tcataatttc ctaacaggag cgatatgaac aacaccagca aaagcactcg tttcagcttt
      781 tcgctcataa catcacctct tgttaaatta tccatcataa cctattaatt atcaatgact
      841 ttaaataata ttcgtgaaat cataaattga cttttctaca gattatattt tccaaattgt
      901 ttgcagattg aaagaattgt tgcgactttt gcttgtttat attattgcct gatgatataa
      961 ttggatgtaa ggatgaagaa aaataatgta ataggatgtt ttatatgtac aaaccaggag
     1021 aactgctaga aagactcata ccattagacg aagacgaact gatttatagg gaattgtttt
     1081 ttgcaaaaca gaataaggat acttatgaag agtttcttag aaaacacgag gatttcagga
     1141 acaacgaact tttgctcgtt gaaaacacca cggctttttc tttttgtctt aaagaagaaa
     1201 acattcttct gtttgacaac ctggaatcaa acatatgtgt gctgaaacac aaaagataca
     1261 gtcccatgag gggacacagc cacacattct ttgaaatgat ttatgttcta aaaggctcgt
     1321 gcgataatat aattgaaggg actccaatca gactgaatga aggagacatt tgcataatgt
     1381 cctctgatgt gactcatgca atgagtgttt ttgacgacag cttgattatc aatatcctta
     1441 taaaaaagac caccttcaag gaaacttttt tcaagctcct taaggacgac aatattcttt
     1501 caacattttt cactcgtatc ctttatacga acaacgcaag caactacata ctgttccata
     1561 caaagggaga cataaggttg cacacccttc ttgaatacct gatctgggat ggaatctttg
     1621 aaggccataa gaacaacagt gatttgagag agtacctttt gcttgcatcc tttcactaca
     1681 tgctggaaaa tcatgtggac aacattgaac ttccaaaaac agtggatacg aacataccaa
     1741 acattgtcca gatactgcgg tatataaaag agaattacaa gactataaag ctgggggatt
     1801 tggcaaggca tttcaactat gctccaaact atatcagcaa cctgataaaa tccacaacag
     1861 gttacacctt ctcggagata cttatagaaa cgaagcttag aaatgcatgc aaattcctaa
     1921 gagatacatc ccttcccgtg catgagattg cacacatggt aggatatgac agcaacgaac
     1981 acttccacag ggtgtttaag aaatatactg gctatacgcc caatgagtat aggactatgt
     2041 aaggaaagga atgtttttta tgaatctacc aagaccggaa tatcccagac ctataatgag
     2101 aagagataac tggatcaatt taaatggcga atgggaattt gaacttgatc ccggccaaag
     2161 cggaagagaa cgtaacatgc acgcaaacgg cgtattttca aagaaaatta ttgttccctt
     2221 ctgtcctgaa agcaaactgt caggtcttga aatcaaggat ttcatgcctt gtgtatggta
     2281 cagaagaacc ttcgagctca aagagttgaa aagaaccctg attcactttg gagcagtgga
     2341 ttaccatgca gaggtatgga ttaatggaca tagcgtcggt acccacgtgg gcggctatgt
     2401 gggttttaca tttgacatca cggattttgt aaaagttgga gtcaatgagg ttgtagtatg
     2461 tgcagcggac gacacaagaa gcggcaaaca gcccatcggc aaacaaagct ttagatatga
     2521 ttcccatgga tgtcactata caagaaccac tggtatctgg cagaccgtat ggctcgagca
     2581 ggtttcggat acatatataa gtaatttgaa gatggtgccg gatataaaca atcaaagaat
     2641 caatataact gttttccttg aaggaaatgt aagagatggc tccaacctca agattgacgc
     2701 caggtcttcg tacgaaggca aggatacggg ttccgtggag gttaaaggaa actggagaca
     2761 ggtctcattt tccatccaac taagcgagct gcatctttgg gaaccactaa aaggaagact
     2821 ttacgacctt gaaattacac tttatgaaaa cgacagtctt gttgatacga taaaaagcta
     2881 tttcggcatg agaaatgtgt cccttacaga caaggccatc cttatcaatg gcaagccggt
     2941 attccagaga ctgattcttg atcaaggctt ctacatggat ggtgtttata cagctccgtc
     3001 cgatgatgag ctgaggaaag acattgaaag gtctattgcc atgggattca acggtgcaag
     3061 gctgcatgaa aaggtctttg agccaaggtt cctttactgg gcagacaaac tgggatattt
     3121 gtgttgggga gagcatgcca actggggtct tgatatttca aatccggaag gcatgatggc
     3181 atttctccca gagtgggttg aaatattgaa cagagacata agtcatcctt ccattgttgg
     3241 ctggtgtcct ttcaatgaaa cccaaggcaa tcaggacaat agaatccttg agtatattta
     3301 taaggtgaca agggagatag accctaccag accaattatt gatacaagcg gctgggttca
     3361 tacaaaattt acagacgtct atgacatgca cgattatgat caggatatag aaagctttgc
     3421 aagaaagctg gatcctttca agactggaga tactcccaat gtcactctgt cagatcctgc
     3481 aacgtacatc aagggtaccc catattttgt
//
LOCUS       DAONVY010000222         3508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18556_length_3508_cov_3.244136, whole genome shotgun sequence.
ACCESSION   DAONVY010000222 DAONVY010000000
VERSION     DAONVY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18556_length_3508_cov_3.244136"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /gene="lepB"
                     /locus_tag="PLI11_06590"
     CDS             <1..873
                     /gene="lepB"
                     /locus_tag="PLI11_06590"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPZ52567.1"
                     /translation="VYDKNEYLRSKATDDIEFESLRFGEQKEPNRISAIELLEFGFTE
                     NKEMSFPPIFDSNPEEYIVEDVNEDNYDDISNDEDDDNDIEVVEIEEKDSFNNGSVII
                     KKVTRKIFSWIFTITFAFVLALLINMYVLRPSKVSGDSMVPTLVDNETVYISRLPYVF
                     GDVKFGDIVVIDSNIKEERTFVKSVAETLKYNLLTKDLFEYEVDVFWIKRVIGVEGDV
                     IEFKDNAIYRNGQKLKEDYIYTQDVFTYPNGVTVTVEEGYIYVMGDNRNVSRDSRHEG
                     LIPVSHVVGKLVFH"
     gene            882..1271
                     /locus_tag="PLI11_06595"
     CDS             882..1271
                     /locus_tag="PLI11_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPZ52568.1"
                     /translation="MEKLTIAMQNYLETIYEIAVNNNDEHSGVRLSDIAAKLNVSKAS
                     VNNAISVLVNAGYVKSEKYQDVFLTENGIETAKLLTSRHEVIKKLFTDVLGVDDETAN
                     EDACAIEHVISREAIQKIKDFLDKHKN"
     gene            complement(1465..2514)
                     /gene="leuB"
                     /locus_tag="PLI11_06600"
     CDS             complement(1465..2514)
                     /gene="leuB"
                     /locus_tag="PLI11_06600"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPZ52569.1"
                     /translation="MDFRIALIKGDGIGPEIVDSATKVLDKIGEKFNHKFNYVDLIAG
                     GVAIDKYGHPLPEETVKECLKSDSVILGAVGGPKWDNLPGELRPERALLGVRKALNLY
                     SNLRPAKLYKALQQYCPLRTASSFDILIVRELTGGIYFGERGRRETENAVEAYDTESY
                     SVMEIERIGRVAFEAARKRNRKLTSIDKANVLESSRLWRETMHKLKEEYKDVEYSDML
                     VDNAAMQLIRDPSQFDVIVTSNMFGDILSDEASQLTGSIGMLPSASLGEGTLGLYEPI
                     HGSAPDIAGTKKANPIATILSASMMLRYSFNLIEEADAIDNAVEKALDIGYTTSDIGG
                     NLNTDEVTQKIIELI"
     gene            complement(2516..3013)
                     /gene="leuD"
                     /locus_tag="PLI11_06605"
     CDS             complement(2516..3013)
                     /gene="leuD"
                     /locus_tag="PLI11_06605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPZ52570.1"
                     /translation="MNAYGKVIKYGDNVDTDVIIPARYLNTTDKKELASHCMEDIDPD
                     FKNKVEPGDIIVAGRNFGCGSSREHAPIVIKESGISLVIAKSFARIFYRNAINIGLAI
                     IECPECIDEMDEGDKVRVDFDKGEIVNETKNKTYKASPFPPFIQTIIQNGGLINSIKN
                     NAFGG"
     gene            complement(3030..>3508)
                     /locus_tag="PLI11_06610"
     CDS             complement(3030..>3508)
                     /locus_tag="PLI11_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPZ52571.1"
                     /translation="DLSKLGPVVAFPHLPENTKTFDQFDDIKIDQVVIGSCTNGRLED
                     LKVAAEVLKGRKVASHVRAIIIPATPKIYLEALEKGYLKIFMNAGCVVSAPTCGPCLG
                     GHMGILAKGERAVATTNRNFVGRMGHVESEVYLASPYVAAASAVTGKISHPREVME"
BASE COUNT          987 a          663 c          756 g         1102 t
ORIGIN      
        1 gtgtatgaca agaatgaata tctgcgcagc aaggcgacgg atgatattga atttgagtcg
       61 ctcagatttg gtgagcagaa agagcctaat agaatatcgg cgattgaact actagagttt
      121 ggatttactg aaaacaagga aatgagtttt cccccgattt tcgattccaa cccggaagaa
      181 tacattgtag aagatgtaaa tgaagataac tatgatgata tcagtaatga tgaagacgac
      241 gataacgaca ttgaagttgt cgaaattgag gaaaaggaca gtttcaacaa cggctcagtt
      301 ataatcaaga aggttacaag aaaaatattt agttggatat ttaccataac gttcgctttt
      361 gttctggcac ttttaatcaa tatgtatgtt ctaagaccca gcaaggtttc cggagattca
      421 atggttccca ctttagttga taatgaaaca gtatatatat caagacttcc ttacgttttt
      481 ggcgatgtta aatttggcga tattgttgta atagacagta atattaaaga agaaagaact
      541 tttgtgaaat ccgttgcgga gactctaaaa tacaacttgc ttacaaaaga tttgtttgaa
      601 tacgaagtcg atgtgttttg gataaagagg gtaattggtg tagaagggga cgttattgag
      661 ttcaaggaca acgcgattta cagaaacgga caaaaactca aggaagacta tatctacacc
      721 caggatgtgt ttacataccc taatggtgta acagttacag tcgaggaagg ttatatttac
      781 gtaatggggg acaacaggaa tgtaagtcgc gattcaagac atgaaggcct tattcctgtt
      841 tctcatgtgg ttggaaaact tgtatttcat taggaggaga aatggaaaag cttactattg
      901 caatgcaaaa ttaccttgaa accatatacg aaattgctgt caacaacaat gatgagcact
      961 caggagttcg tttaagcgat atcgcggcaa agttaaatgt gtcaaaagca agcgtaaaca
     1021 atgcaattag tgttcttgtc aatgcaggtt atgtaaagag tgaaaaatat caggatgttt
     1081 tccttacaga gaacggtatt gaaacagcaa agcttttaac cagtaggcat gaagtaatta
     1141 agaagctttt tacggatgtc cttggtgttg atgatgaaac agcaaatgaa gatgcttgcg
     1201 ccatagaaca cgttataagc agggaagcaa tacagaagat aaaggatttt ctggataaac
     1261 ataaaaacta ataaaaggaa agaaaacagc atggttgaag tgaccgtgct tttttatatt
     1321 ggttgacttt ggactatgtt tgaaatggga agaaatacaa ttttataaag aggtggctta
     1381 gataatatga acgggaaata gtgtccgaca acaatggcag attcaacata ctaaaaaaga
     1441 ccggatgggt tccggtcttt tgggttatat aagttcaatt attttttgag ttacctcatc
     1501 tgtattcaga ttgccgccta tgtcgctggt ggtgtagcct atatcaaggg ctttttccac
     1561 cgcattgtct atggcgtctg cttcctcgat aaggttgaaa gagtatctaa gcatcatgct
     1621 ggctgaaaga attgtggcaa ttggatttgc cttctttgtg cctgcaatgt ctggagcgga
     1681 accgtgtatt ggctcataca ggcctagtgt tccctctcca agagatgctg atggaagcat
     1741 tccaattgag cctgtaagct gtgaagcttc atcggataga atgtctccaa acatattgga
     1801 ggtaactatt acatcaaact gggatgggtc gcgaataagc tgcattgctg cgttgtctac
     1861 cagcatatcg ctgtattcaa catccttgta ttcctcttta agtttgtgca ttgtttctct
     1921 ccaaagtctg gaactttcaa gcacatttgc cttgtcgatg cttgtaagtt ttctgtttct
     1981 ttttcttgca gcctcaaaag caactcttcc tattctctca atctccatta cactgtaact
     2041 ctcagtgtcg taggcttcaa ctgcattttc ggtttctctt cttcctcttt caccaaaata
     2101 gatgccgcct gtaagttctc ttacaatcag tatgtcaaag cttgatgcgg ttctaagagg
     2161 gcagtactgt tgaagagcct tgtagagctt tgctggtctt aggttggagt agaggtttaa
     2221 agctttcctt actcccagaa gagctctttc cggtcttaat tcacctggga ggttgtccca
     2281 tttagggcca cctacagctc ctagaataac gctgtcgctt ttcaggcact cttttacagt
     2341 ttcctcagga agagggtggc catatttatc tatggcaact cctcctgcaa taagatcaac
     2401 gtagttgaat ttatgattaa acttttcgcc gattttgtcg agtactttgg tggcactgtc
     2461 cacaatttca ggacctatgc cgtcaccttt tatcaatgca attctaaagt ccatgttaac
     2521 ctccaaaggc attgttctta attgagttta ttagtccgcc attttgtata atggtctgga
     2581 taaaaggagg gaagggagat gctttataag ttttgttctt ggtttcgttg acgatttcac
     2641 ctttgtcaaa atcgacacgt accttgtcac cttcgtccat ttcatcaatg cattccggac
     2701 attcaataat tgcaagcccg atatttattg catttctgta gaaaatgcgg gcaaagcttt
     2761 ttgcaattac cagtgaaatg ccactttctt ttattacaat tggggcatgt tctcttgaag
     2821 aaccgcatcc aaagtttctt cctgcaacaa ttatgtctcc tggttctacc ttgttcttga
     2881 aatcaggatc aatgtcttcc atgcagtggg aggcaagttc ctttttgtca gtagtgttaa
     2941 ggtatcttgc agggataatg acgtcggtat ccacattgtc gccgtatttt attactttac
     3001 cgtaagcatt cattgttcgt ctctccttcc tattccatta cttctcttgg atgagatatc
     3061 ttgcctgtga cagcgctggc agcagctaca taagggcttg caagatatac ttcggattca
     3121 acatggccca ttctacctac gaagttcctg ttggttgtgg caaccgctct ttctcctttt
     3181 gcaagaattc ccatgtgacc gccaagacat ggtccgcagg ttggagcgga gaccacgcat
     3241 cctgcattca tgaatatctt caggtatcct ttttcaaggg cttcaagata gatctttggg
     3301 gttgctggta ttatgatggc ccttacgtga cttgcaactt ttcttccttt taagacttcc
     3361 gctgcaactt taaggtcctc aagtcttccg tttgtgcagg aaccgattac aacctgatca
     3421 atcttgatgt catcaaactg atcgaaggtc ttagtgtttt ctggcaggtg agggaaggcc
     3481 acaacaggtc ccagttttga aagatcta
//
LOCUS       DAONVY010000223         3504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18580_length_3504_cov_4.280951, whole genome shotgun sequence.
ACCESSION   DAONVY010000223 DAONVY010000000
VERSION     DAONVY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18580_length_3504_cov_4.280951"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLI11_06615"
     CDS             <1..478
                     /locus_tag="PLI11_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HPZ52572.1"
                     /translation="VSQLKQANAMDKFNDVLNEVPRVREDLGFPPLVTPTSQIVGTQA
                     VLNVLSGERYKMVPKETKGLVKGEYGKTPAPIKDEIKELILKGEKEITCRPADLIEPE
                     LDKIREEIKEYIEQDEDVLTYALFPQPAVNYFKYRQAQKYNVDASEADFENSVHPV"
     gene            532..1755
                     /locus_tag="PLI11_06620"
     CDS             532..1755
                     /locus_tag="PLI11_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPZ52573.1"
                     /translation="MYDCIIIGAGAAGLFSALSASETGKRVLILEKNNIPGKKIRITG
                     KGRCNITNSCSFDELIENIPGNGKFLYSSLSRFSNYDIIDTFNRLGLETVVERGNRVF
                     PASNKSIDVVNVLVKELERRNVEIRYNSCVCKIVYENEYVKSVYVNDQFIETKNLIIA
                     TGGLSYPGTGSTGDGYRFAIEAGHTIVPTKPSLVPLVSSDQWIKELQGLSLKNIRLSL
                     INKDGCFYSDFGEMLFTHNGISGPLVLTGSRHYISQQYKEIIASIDLKPALDEEKLDS
                     RLLRDFEKYSRKQLKNAFNDLLPAKMIPVFVNRLDIDDNKTVNQITSYERKQILTLLK
                     DFRINIISHGKMAEAVVTSGGVCTKEINPKTMESKIKKGLFFAGEVIDVDGYTGGFNL
                     TVAFSTGYLAGKSLN"
     gene            1812..2273
                     /locus_tag="PLI11_06625"
     CDS             1812..2273
                     /locus_tag="PLI11_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52574.1"
                     /translation="MSKFKVFIISLWILTLIFVFNGFGYKGIEFDDIVQNYPETELAS
                     LNDARMNFKEGHGIIQIKVTNLSSSGKKRGGVYVSVNGTIKLNITTQDPRNLRVKAND
                     IIIVKGHDLAGNATVTISDVAGFIEHDVKGQTIEVGNLAKYFVTIKPQPMD"
     gene            2326..2814
                     /locus_tag="PLI11_06630"
     CDS             2326..2814
                     /locus_tag="PLI11_06630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPZ52575.1"
                     /translation="MKAIIEMENKDRIEIELYEDIAPVTVANFVKLAEEGFYNGLIFH
                     RVIKDFMIQGGCPYGTGTGGPGYTIKGEFNANGFLNPLKHTRGVISMARAFDPNSAGS
                     QFFIMHKDAPHLDGSYAAFGKVVKGIEVVDKIAETETDMRDKPFADQKINKIIIERGD
                     VN"
     gene            2811..>3504
                     /gene="galE"
                     /locus_tag="PLI11_06635"
     CDS             2811..>3504
                     /gene="galE"
                     /locus_tag="PLI11_06635"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPZ52576.1"
                     /translation="MTILVTGGAGYIGSHACVELLNSGHDIVVFDNYYNSSPEVLDRI
                     KEITGRSFLAYECDMLDEEAMERIFSENKIDAVMHFAGYKAVGESCEKPLMYYHNNIQ
                     GTLTILNAMARHNVKKFVFSSSATVYGDPQSVPIDETFPLSATNPYGRTKLMIEEMLR
                     DLYAADNSLSIILLRYFNPVGAHESYMIGENPKGIPNNLMPMINKVAQQELPVLKIFG
                     NDYNTKDGTGVRD"
BASE COUNT         1230 a          555 c          746 g          973 t
ORIGIN      
        1 ggtttcacag ctcaagcagg ccaatgccat ggacaaattc aacgatgttc tgaatgaagt
       61 accaagagtt cgtgaagatt taggattccc tccacttgtt actcctacaa gccagattgt
      121 tggaacgcaa gctgttttga atgtgcttag cggtgagaga tacaagatgg tgccaaaaga
      181 aacaaagggg ctggtaaagg gtgaatatgg aaaaactcca gcaccaataa aggatgaaat
      241 taaagagctt atcttaaaag gtgagaagga aattacatgc agacctgctg atttgattga
      301 acctgagctt gacaagataa gggaagaaat caaggaatat attgagcagg atgaagacgt
      361 tctcacttac gctcttttcc cacagccagc agtaaattac tttaaataca ggcaggcaca
      421 aaaatacaac gtggatgcgt ctgaagcaga ttttgaaaat tcagtacatc ctgtataaga
      481 aaagatttgc gcttatatta ttttaagcgc aatatttttt agggagaaaa tatgtacgat
      541 tgtataataa ttggggccgg tgcggctggt ctattttcag ctctgtccgc atcggaaaca
      601 ggaaaaagag ttctgatact tgaaaaaaac aacataccag gtaaaaagat aaggataaca
      661 ggaaagggta gatgcaacat taccaattcc tgttcttttg atgaattgat tgagaatatt
      721 cccggaaatg gtaagtttct ttactcaagt ttatcaaggt tctcaaacta cgacataatt
      781 gataccttta ataggctggg actagagaca gtggtcgaaa ggggaaacag ggtgtttcct
      841 gcatccaata aatccattga cgttgttaat gtacttgtaa aagaacttga aagacgaaac
      901 gttgaaataa gatataactc ctgcgtttgc aaaattgttt atgaaaatga atatgttaag
      961 agcgtatatg taaatgacca attcattgag accaagaatt taataatagc tacgggaggc
     1021 ttatcttatc ccggaacagg atccacagga gatggataca gatttgcaat agaagcagga
     1081 catacgatag ttcctacaaa accttctctt gtcccacttg tctcatcaga ccaatggatt
     1141 aaggagctgc aagggttgtc attaaagaat ataagattgt cgttgataaa taaggatgga
     1201 tgtttttaca gcgattttgg tgaaatgctg tttactcata acggtatatc aggacctttg
     1261 gttcttacag gcagccgaca ttatatctca caacaataca aagaaattat agccagcata
     1321 gacttgaaac cagctcttga tgaagaaaaa ctagacagta gactattaag agactttgaa
     1381 aaatattcga ggaaacagct aaaaaatgct ttcaatgatc ttttgcctgc aaaaatgatt
     1441 ccagttttcg taaacaggct tgatattgat gataataaga ctgtaaacca gataaccagt
     1501 tatgaaagaa agcaaatact aacactacta aaagacttca ggataaatat cattagtcat
     1561 ggtaaaatgg ctgaagctgt ggtaacatca ggtggtgtat gcacgaaaga aattaatcct
     1621 aaaacaatgg aatcaaagat aaagaaagga ttattctttg caggtgaagt aattgatgtt
     1681 gacggatata ccggtggatt taatttgaca gttgcttttt ccactggata tctggcaggc
     1741 aaatctttaa actagtgaat atatttttta tttgctcata aaatttataa agcatgattt
     1801 ttggtgatat tatgagcaaa ttcaaagtat ttattatatc tttatggata ctaacattga
     1861 tatttgtttt caatggcttt ggttataaag gcattgagtt tgatgacatt gtacagaatt
     1921 atccagaaac agagctggca agcctaaatg atgcgagaat gaattttaag gaaggtcacg
     1981 gtatcataca gattaaggta accaacctca gcagcagtgg aaagaaaaga gggggagtat
     2041 acgtatctgt aaacggtacg ataaagctta acattacaac tcaggatcct agaaacttaa
     2101 gagtcaaggc aaacgatata attattgtaa aaggccatga tctggctgga aacgcaacag
     2161 tgacaatatc agatgttgca ggattcattg agcatgatgt aaaaggtcag acaatagagg
     2221 ttggaaatct ggcaaaatat tttgttacaa tcaagcctca gccaatggat tgacaataat
     2281 ttggcgggga tatatactaa taaagttaat atgtggaggt aatttatgaa agcaataatt
     2341 gagatggaaa acaaggatag aatcgaaatt gaactatatg aagatattgc tccagttact
     2401 gttgcaaatt ttgtgaaact ggctgaagaa ggtttctaca atggactaat attccataga
     2461 gttattaaag acttcatgat acagggtgga tgcccttatg gtacaggaac aggaggtccc
     2521 ggatatacta tcaaaggaga gttcaatgcg aatggattcc tgaacccttt gaaacataca
     2581 cgcggtgtta tctcaatggc aagagccttt gatcctaatt cagcaggctc acaattcttc
     2641 atcatgcaca aagatgcacc tcaccttgat ggaagctatg ccgctttcgg caaagtagtg
     2701 aaaggcattg aggttgtaga caaaattgct gaaactgaaa ctgacatgag ggacaagcct
     2761 tttgcagatc agaagataaa taaaataata attgagagag gcgatgtaaa ttgactattc
     2821 ttgtaactgg tggtgccgga tacatcggaa gccacgcctg cgttgagtta ttaaatagtg
     2881 gtcatgacat tgttgttttt gacaattatt acaacagttc tccagaagtt ttggacagaa
     2941 tcaaggaaat aaccggaaga tcatttttgg catatgaatg cgacatgctt gatgaagaag
     3001 ccatggagag aattttttcc gagaacaaga ttgatgctgt tatgcatttt gcaggataca
     3061 aggcagtagg ggagtcctgt gaaaaacctc tcatgtatta tcacaataat atccaaggaa
     3121 cccttaccat attgaatgca atggcaagac acaatgtaaa gaaatttgtc ttcagctcct
     3181 ctgcaacagt ttacggtgat cctcaaagcg ttcccataga tgaaacattc cctttatcag
     3241 ctacaaatcc atacggaaga actaaattaa tgattgagga gatgttaaga gatctctatg
     3301 ctgcagacaa ttcattgtct attattctgc ttagatattt taatcctgta ggcgctcatg
     3361 agagttatat gataggagag aatccgaaag gaatacccaa taaccttatg ccaatgataa
     3421 ataaagtagc gcagcaagaa ctccctgtat taaagatttt tggtaatgat tacaatacaa
     3481 aagacggaac aggtgtaaga gact
//
LOCUS       DAONVY010000224         3497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18620_length_3497_cov_4.029924, whole genome shotgun sequence.
ACCESSION   DAONVY010000224 DAONVY010000000
VERSION     DAONVY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18620_length_3497_cov_4.029924"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1219)
                     /locus_tag="PLI11_06640"
     CDS             complement(83..1219)
                     /locus_tag="PLI11_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPZ52577.1"
                     /translation="MKDKMTLVATATFGLEAMVSRELKWLGYTDQHTDNGRIYFTGDA
                     EAICKTNMWLRCANRIYLQVGKFKAETFEELFQNTKALPWEEYLPVDANFPVDGKSAK
                     SKLFSVSDCQAIVKKAIVERLKLFYDKEIFPETGARYKIEVALQKDIATLSIDTTGHS
                     LHKRGYREEVGDAPIKETLAAGLLYVSRWKPDRALLDPMCGTGTIPIEAALIGMNIAP
                     GLNQTFDAENWKQMDKSLWKKTREDARSRINHDVNLRIYASDINYFAIKSAMENAKRA
                     GVFDKIHFQKMDFRETSSRYKYGFIITNPPYGERLSRDDDIVKLYKDMGNHFKTFETW
                     SYYIITPHEKFESLFGRRADKKRKLFNGNIKCDYYQFYGPKPPF"
     gene            complement(1216..1953)
                     /locus_tag="PLI11_06645"
     CDS             complement(1216..1953)
                     /locus_tag="PLI11_06645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPZ52578.1"
                     /translation="MTEIYRFDGKTTSNEIKIIYEDNHIISVVKPAGILSQGDASHDT
                     DLLSLLKQDLKTRYNKPYDAFVGLVHRLDRNTGGTMVYAKTSKGASRLSKQLRDKEFK
                     KGYFAIVDGIVKKDGRLTNKLEKDSKTNKVYESDEGKLSILEYKVIDCIGDMSLVFVI
                     PITGRTHQIRAQMAFFGHPLIGDVKYGYKKNMGDFFALWSSCIKIKHVTLDKKLLLQS
                     IPERSNVWSKFKEKTYIDALNEIGDVL"
     gene            complement(1968..3152)
                     /locus_tag="PLI11_06650"
     CDS             complement(1968..3152)
                     /locus_tag="PLI11_06650"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HPZ52579.1"
                     /translation="MDKPKILDMNVGEMPNYRYLCTCGREHGVDINRIEMGKGVIEKL
                     PEIIQKFKDKTVLIVCDDNTFKIAGERVQNILESNGFKTRIHVAYSLDYPILIPDAFT
                     VGRLMIAAEDDTGLILGVGSGTINDVCKIVSYKMKLDSVIVCTAPSMDGYASTLSPLI
                     VDKQKITYPSHYPYAIVADVDIMKDAPLEMLRAGFGDIVGKYTALTDWRLTVIVNKEY
                     YCDTTAKLMQNAVDKVVENTERYFAREADAIYNVTEALMLAGISMGLVGISRPASGSE
                     HHLAHFWELDALAKGIEHPLHGNSVGVGSIITSEAYRIMSEKFEEPKAANPPSPELLR
                     DIYRRADMPLTPKELGIEKDVFHNSIRNAYRIRPRYTIFNYTKDRALLDKMADDLTDI
                     FY"
     gene            complement(3227..>3497)
                     /locus_tag="PLI11_06655"
     CDS             complement(3227..>3497)
                     /locus_tag="PLI11_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52580.1"
                     /translation="VSPLDNPYDSKPVHASKKKKPGGIGSFIENILDRIKQILSRFLG
                     RDVGTDDLLLLGLILVLFFEKRNKDSSDSDDIDLLLIVLLYLLIS"
BASE COUNT          907 a          794 c          615 g         1181 t
ORIGIN      
        1 atttcctcct taataaaatt tacttacttt tcgcatttta ttccatttta cttaaactgt
       61 caagccttat tattattttg catcaaaaag gcggctttgg tccgtaaaat tgatagtaat
      121 cgcacttgat gtttccattg aagagttttc tctttttgtc agccctcctt ccaaacagac
      181 tttcaaattt ctcgtgcggc gttattatat agtatgacca tgtttcaaag gttttaaaat
      241 gatttcccat atctttgtat aattttacta tatcgtcgtc tcttgacagt cgctcgccgt
      301 aaggtggatt ggtgataata aatccgtatt tgtacctgga agaggtctct ctaaagtcca
      361 tcttctgaaa atggatttta tcaaacaccc ctgccctttt ggcattttcc atagcacttt
      421 taatagcaaa gtagtttatg tcggaagcat agatgcgcaa gttgacatcg tgatttatcc
      481 tgctcctggc atcttccctg gttttcttcc acaaggactt gtccatctgc ttccaatttt
      541 cagcgtcaaa agtctggttc aaacccggtg caatattcat tccgattaat gcagcttcaa
      601 tgggaattgt gcctgtacca cacatcggat ccaacaaagc tctgtcaggt ttccacctgc
      661 ttacatacaa aaggccggct gcaagagtct ctttgattgg agcatcccct acttcttcac
      721 ggtatcccct tttgtgcaga ctgtgtcctg ttgtgtcaat tgacaatgtt gcaatatcct
      781 tctgcagtgc aacctcgatt ttatatcttg cacccgtctc agggaaaatc tccttgtcgt
      841 agaaaagctt caatcgctca acgatggctt tcttaactat agcctgacag tcggatacac
      901 tgaacagttt tgactttgca ctctttccat ccactggaaa gtttgcgtca acaggaagat
      961 attcttccca aggcaatgcc ttggtgtttt gaaacagttc ttcgaaagtc tcggctttga
     1021 acttgcccac ttgcaaatat attctgtttg cgcatcttag ccacatgttt gttttgcata
     1081 tggcctcggc atctccggta aagtatattc ttccgttatc agtatgctga tcagtatatc
     1141 ccagccattt cagttctctt gacaccatgg cttccagtcc aaatgtagcg gtagcaacaa
     1201 gagtcatttt gtctttcata aaacatctcc tatttcgttt aatgcatcaa tatatgtttt
     1261 ttccttgaac ttgctccata cattagacct ttcaggtata ctttgcaaaa gaagtttttt
     1321 gtctaatgta acatgtttta tcttgataca ggaagaccac agggcaaaaa aatctcccat
     1381 gttcttttta tacccatatt tcacatctcc tattaaagga tgaccgaaaa aggccatctg
     1441 agcccttatc tgatgggttc ttcccgtaat cgggattaca aacaccaacg acatatctcc
     1501 gatgcaatca ataactttgt attcaagtat tgacagtttc ccctcgtcgg attcatacac
     1561 cttgtttgtt ttactgtcct tttcaagctt gtttgtcagt ctgccgtcct ttttcacaat
     1621 tccgtcaact atggcaaagt atcctttttt gaattccttg tctctaagct gctttgacaa
     1681 tcttgaagct cccttggatg tctttgcata taccatggtt ccaccagtat ttctgtcaag
     1741 acgatgaaca aggcctacaa aagcgtcata aggtttgttg taccttgtct tcaaatcctg
     1801 tttcaaaagg gaaaggagat ctgtgtcatg tgacgcatcc ccctggctca aaatgcctgc
     1861 aggcttgaca actgagatta tatggttgtc ctcgtatata atctttattt cgtttgatgt
     1921 cgtttttccg tcaaatctgt agatttcagt catcaagcac ctcttaatta atagaatatg
     1981 tcggtaaggt catctgccat cttgtccagc agagccctgt ctttcgtata attgaaaatc
     2041 gtataccttg gtcggattct gtaagcgttt cttatactgt tatggaatac gtccttctca
     2101 atgcccagtt ccttgggtgt aagaggcata tccgctcttc tgtatatatc ccttagcagc
     2161 tcgggacttg gcggatttgc agctttcggc tcctcaaact tttcgctcat gattctgtac
     2221 gcttccgaag taattatcga gcctacacct acagaattgc catgaagagg atgttctatt
     2281 cctttggcaa gtgcatcaag ctcccaaaag tgagcaagat gatgctcgct tcctgaagca
     2341 ggtcttgata tgcctacaag gcccattgag atgcctgcca gcatcaaggc ttcagttaca
     2401 ttgtaaatgg catccgcttc tcttgcaaaa tacctttcag tattctccac caccttgtcc
     2461 actgcattct gcatcagctt tgcagtggtg tcgcagtagt actccttgtt gacgattact
     2521 gtaagtctcc agtctgtaag agcggtatat ttgccaacaa tatcaccaaa acccgctctg
     2581 agcatttcca atggtgcatc cttcattatg tcaacatcag caactattgc ataaggataa
     2641 tgggatggat acgtaatttt ctgcttgtct acgataagtg gagacaaagt tgacgcatag
     2701 ccgtccatgg aaggagcggt acatacgata acgctgtcta gtttcatttt atatgaaacg
     2761 attttgcaaa catcatttat tgtacctgaa ccaacaccaa gaattagacc cgtatcatct
     2821 tcagctgcaa tcattaatct tccaactgta aaagcatcag gaatcaatat tggatagtct
     2881 agtgagtatg caacatgtat tcttgtttta aaaccattgc tttcgagtat attctggact
     2941 ctttcgcctg caatcttaaa tgtgttgtca tcacatacta ttaatacagt tttgtctttg
     3001 aacttttgaa tgatttcagg aagcttttca attacaccct tccccatctc aatcctgttt
     3061 atatcaacgc cgtgttctct gccacatgtg caaagatacc tgtagtttgg catttcacca
     3121 acattcatgt ccaatatttt tggcttgtcc attttttaaa ctccttttta cattgtctta
     3181 ctttacaata gtaacatatg gcagcgtcca tatcaataat ttgatattaa gatatcagca
     3241 aatacaatag aacaatcagt aacaagtcga tatcgtcgct gtctgaggaa tccttgtttc
     3301 ttttttcaaa aaacagcaca agaataaggc caagaagtag caagtcatct gtacctacat
     3361 ccctgcccaa aaaccttgac aatatctgtt tgattctgtc cagtatgttt tcaataaagc
     3421 ttccaatgcc gcctggcttt ttctttttgg aagcatgtac tggtttggaa tcataggggt
     3481 tatccagagg agatacc
//
LOCUS       DAONVY010000225         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18676_length_3488_cov_3.843868, whole genome shotgun sequence.
ACCESSION   DAONVY010000225 DAONVY010000000
VERSION     DAONVY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18676_length_3488_cov_3.843868"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLI11_06660"
     CDS             <1..678
                     /locus_tag="PLI11_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPZ52581.1"
                     /translation="NDKEKLNEEQMKLYQKYNINPMAGCLPTLIQFPLIISLYRIVTQ
                     PLTYISGVSITAITALQKFVANAQNISKSKAVSEIVINDFFLKNTDKLAEVSEHITGG
                     QLLNMKFLKIFDLGMTPSLSKLFSDPKVYLPLLLIPIISALTSYLIQKTTTANSANKK
                     KKEDEKEKASNPLETMTKIMPIISLLITFNVPAGVGLYWIIGNVLGIGQNYITKKVFQ
                     KKKEGNV"
     gene            678..1352
                     /gene="jag"
                     /locus_tag="PLI11_06665"
     CDS             678..1352
                     /gene="jag"
                     /locus_tag="PLI11_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPZ52582.1"
                     /translation="MAKSVEKSGRTVDEAINAALAELNIDLEDAEVDILDEGSKAVLG
                     LFGGKEARVRVTENKPDSKVLREFLGIVVDTMDVEVRLNIENNEDALRADIYGEDVAF
                     LIGRHGDTLQALNYLSNLIINKEKENYRRVIVDVEDYRKKKEENLRSLANRTAAKVVR
                     YRRPVSLDPMQAYERRIIHTELQNHEYVETVSQGEEPNRCVTVRLKPNANLRNNKYRG
                     SYRKRY"
     gene            1352..2734
                     /gene="mnmE"
                     /locus_tag="PLI11_06670"
     CDS             1352..2734
                     /gene="mnmE"
                     /locus_tag="PLI11_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPZ52583.1"
                     /translation="MNYVEDTIAAISTPPGRGGIGIIRISGKESVAIANRIFCMAYGR
                     LGDFVLNMPTHTIKYGFIRDPESGEILDECMLTRMDAPNTYTKEDVIEINCHGSYAVL
                     RRVLELVFRMGARSAEPGEFTKRAFLNGRIDLSEAEAVMDLINSHTEEARKAAIDQLG
                     GRLSKQIERIRQGLVSVLAEIEVNIDYPEYESELVTADVTMKTLNSVIIELEDLSKSY
                     RQGRLIREGLKITIAGKPNVGKSSLMNALTGYNRAIVSSIPGTTRDTIEEYIEIDGIP
                     VILTDTAGIRETEDVIEQIGVKRAEEKIEEADIVIYMIDSTEPDIEEKILSIKKPLIV
                     ILNKIDIETNYLEELGRKLKNYDVIETSLTEDIGVKEVFDRIKKYFSEEKIGINTENI
                     ITNARHKTLIDTSLASLKKAVENYNTGMPLDIIAFDIWNGVNTLGEITGASVNEEVLN
                     NIFSRFCLGK"
     gene            2748..>3488
                     /locus_tag="PLI11_06675"
     CDS             2748..>3488
                     /locus_tag="PLI11_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ52584.1"
                     /translation="MRFIAGEYDVAVIGAGHAGCEAALACAKLGVKTVIFAMNLDSVA
                     NMACNPSIGGSAKGQLVREIDALGGVMAKIVDKSAIQIRMLNKGKGPAVQSLRAQIDR
                     RKYQQEMKHVLELQENLDLKQAEIVDMETVEEDGVRKVTHVVTHTGAVYKCKSAIVCT
                     GTFLNGRIIIGDVSFQSGPDGLFPSIRLSDTLTNLGFKLIRLKTGTPARVNRRSIDFS
                     NMVPQPGDEEAVPFSFETEELEIDQIPCW"
BASE COUNT         1231 a          587 c          812 g          858 t
ORIGIN      
        1 aatgacaagg aaaaattaaa tgaggagcag atgaagttat accaaaagta taacataaat
       61 cccatggcag gttgcttgcc tacgttgatt caattcccac ttattatttc gttgtatcgc
      121 atagtaaccc agccattgac ctatatatcc ggtgtttcca ttaccgcaat aaccgcactt
      181 caaaaatttg tggcaaatgc ccagaacata tcaaaaagta aagctgtaag cgaaatagta
      241 atcaatgact ttttcctaaa gaatacggac aagctggcgg aagtgtcgga acacattacc
      301 ggaggacagc tcctgaacat gaagttcttg aagatatttg atcttggtat gacgccatcc
      361 ctgtcaaagt tgttttcaga tccaaaggtt tatttgccct tgcttttgat accaattata
      421 tccgcactaa cgtcatatct tatacaaaag accacaacag caaacagcgc taataagaaa
      481 aagaaggaag acgaaaaaga gaaagcatct aatcctttgg aaacgatgac caaaatcatg
      541 ccgataatat cattgctcat tacattcaat gttcctgcag gtgtaggtct gtactggata
      601 ataggcaatg tacttggaat aggacagaat tatataacta agaaagtttt tcaaaaaaag
      661 aaagaaggta atgtataatg gcaaagagtg ttgaaaaaag cggacgtaca gtagacgaag
      721 ccattaatgc ggctttggcg gaactcaata ttgaccttga agatgcggaa gtagatattc
      781 ttgatgaagg ttcaaaagca gtattgggtt tgtttggagg caaggaagct agagtcagag
      841 ttacggaaaa caaaccggat agtaaggtat tgagagagtt tttaggtatt gttgtggata
      901 caatggacgt tgaggtaaga ttgaatattg agaataacga agatgcattg agggctgaca
      961 tctatggcga ggatgttgca ttccttatag gcaggcatgg agacactctc caggcgctta
     1021 attatctctc caacctgatt atcaacaagg aaaaggaaaa ttacagacgt gttattgttg
     1081 atgtggaaga ttacagaaag aagaaagaag aaaatctaag aagccttgca aacagaaccg
     1141 cagcaaaagt tgtcagatac agaagacctg tttctcttga tcctatgcag gcatatgaaa
     1201 gaagaataat ccacactgaa ctgcagaatc atgaatatgt agagaccgta agtcagggag
     1261 aagagccaaa caggtgtgta acagttagat tgaaacctaa tgcaaatctt cgcaacaaca
     1321 aatacagagg ttcatataga aagagatact aatgaactat gttgaagaca caatagcagc
     1381 aatttcaaca ccgcccggaa gaggcggaat cggcataata agaataagtg ggaaggaatc
     1441 agtagccatt gcaaacagga tattttgcat ggcttatggt cgtttagggg actttgttct
     1501 aaacatgcca acccacacca taaagtatgg atttatcagg gatccggaaa gcggcgagat
     1561 tcttgacgaa tgtatgctga cgagaatgga tgctcctaac acatatacca aggaagatgt
     1621 tatagagata aactgtcatg gcagctatgc agtattaaga agggttctgg agctggtatt
     1681 ccgcatggga gcaagaagtg ccgagccggg tgagtttaca aagcgtgcat ttttgaacgg
     1741 tagaatagac ttgtccgaag cggaagctgt catggaccta ataaattctc ataccgaaga
     1801 ggctagaaaa gcagcaatag atcagcttgg tggaagattg tcaaaacaga tagagagaat
     1861 cagacagggg ctggtctcgg tgcttgctga aattgaagta aatatagatt atccggaata
     1921 cgaatctgaa ctcgttactg cagatgtaac aatgaaaacc ctgaatagtg ttattattga
     1981 acttgaggat ctgtctaaaa gctacagaca gggaagattg ataagggaag gcctgaagat
     2041 aactattgca ggaaaaccaa acgtaggcaa atcgtccctt atgaacgcac ttacaggata
     2101 caacagggca atagtatctt ccatacctgg aaccactagg gatacgattg aagaatatat
     2161 tgaaattgac ggaatacctg ttattttaac tgataccgcc ggtatcaggg aaacagaaga
     2221 tgtaatagaa caaataggag ttaaaagggc agaagaaaag atagaagaag cagatattgt
     2281 aatatacatg atagacagta ccgaacctga tattgaagaa aagatactgt ccataaagaa
     2341 accgctcata gtaatactca acaaaattga cattgaaacg aattacctcg aagaacttgg
     2401 aagaaaatta aagaattacg acgttattga aacatcactt acagaagaca taggcgtaaa
     2461 agaagttttc gacaggataa aaaagtattt ttctgaagag aaaattggaa taaacactga
     2521 aaacataatt acaaacgcaa gacacaaaac ccttatagac acaagccttg catcactaaa
     2581 gaaagccgtt gaaaactaca acactggaat gcctctggat attatagcct ttgacatatg
     2641 gaatggcgtg aatactttag gagagattac aggggcttcg gtaaacgaag aagtattgaa
     2701 caacattttt tcgagatttt gtcttggtaa ataggaaggg aacaaaattg agatttatag
     2761 ctggagaata tgatgtcgca gttattggag caggccatgc aggttgcgag gctgccttgg
     2821 cttgtgctaa acttggcgtt aaaaccgtaa tatttgcaat gaatctggac agtgtagcca
     2881 acatggcatg caatcccagt attggtggct cggcaaaggg tcagctggtt agagagattg
     2941 atgccttagg tggcgttatg gccaaaattg tagataaatc agcaattcag ataaggatgc
     3001 tcaacaaggg aaaaggacct gctgtacagt ctttaagagc tcagatagac aggagaaagt
     3061 atcagcagga aatgaagcat gttctggaac tacaggaaaa ccttgatctg aaacaggctg
     3121 aaattgttga tatggaaact gttgaggaag atggagtaag gaaggttacc catgtggtga
     3181 ctcataccgg tgctgtctac aaatgcaagt ctgccattgt atgcacgggt acttttttga
     3241 atggaagaat aataattggt gatgtcagtt tccagagcgg gcctgacgga ctgtttccat
     3301 ccattcgatt gtctgatact ttaacaaatc tgggatttaa attgataaga cttaaaacag
     3361 gtactcctgc cagggtaaat agaaggagca tagacttttc caacatggtt ccccagcccg
     3421 gagatgagga ggcagtcccc ttctctttcg agaccgaaga attggaaata gaccagatac
     3481 cctgctgg
//
LOCUS       DAONVY010000226         3485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18695_length_3485_cov_3.181341, whole genome shotgun sequence.
ACCESSION   DAONVY010000226 DAONVY010000000
VERSION     DAONVY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18695_length_3485_cov_3.181341"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLI11_06680"
     CDS             complement(<1..386)
                     /locus_tag="PLI11_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPZ52585.1"
                     /translation="MKLNEIMTTNVISVDQNESVKKAAELMKQHGIGAVPVCMDDQKV
                     IGILTDRDIVLRSIAVGKDVNSLTVKDIMTLNPVTATPEIDVHEAARIMSVKQVRRLP
                     VVSNDRLVGIVSLGDISVEPKLSDNA"
     gene            complement(535..1482)
                     /locus_tag="PLI11_06685"
     CDS             complement(535..1482)
                     /locus_tag="PLI11_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ52586.1"
                     /translation="MKKLFMILMVMSLTLVVLTGCGYLFNPPVETDDGDNNGNTQIKV
                     IHNKGEFTINKVARKVVVFDMGALDTIDALGAEVDLGVPTSNIPTSLSKYKNAKDMGT
                     LFEPDLEAIFNFEPDVIIIGTRQQDYYEQLTEIAPTMFVDLRPEAFMEDFKQNTQNIA
                     SILGLSNKADDHINEIDATIEYLKLKADNSEKKALTLLTNDGNISVYGKGSRFGLIHD
                     VLKIKTADDTIETSTHGQSASFEYIAQVNPDIIFVVDRTVVVGGTVKASDTLNNDLVK
                     STNAAKENMIVYLDPDAWYLASGGLNTMAVMLSDIQKAF"
     gene            complement(1537..2295)
                     /locus_tag="PLI11_06690"
     CDS             complement(1537..2295)
                     /locus_tag="PLI11_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPZ52587.1"
                     /translation="MIRSENLVKSYGEKVVLNNVSLNLPKGKMIAFIGANGAGKSTLI
                     SLMSRIVPKTEGCVYVDNKELQQWNNNELSKRISILKQSNHLNIRLTVRELISFGRFP
                     YSKGRLTKKDNEIIDKALSFLSLDNIQHRNIDELSGGQRQMAYIAMIIAQDTEYVFLD
                     EPLNNLDMRHSVQIMKGLRKLVDEMNKTIMVVIHDINFIAYYSDYIIAMKDGKVVSQG
                     YTDEIITKEVLKEIYGMDIDVQSVNGKPICLYYN"
     gene            complement(2292..3278)
                     /locus_tag="PLI11_06695"
     CDS             complement(2292..3278)
                     /locus_tag="PLI11_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPZ52588.1"
                     /translation="MPRYAMEVIKKHKKTIAIAAFFTFVLLLTGSLFLFQGLTTKNAY
                     FFFPRRLSKIAAVLLVGYCVSYSSVTFQTITNNRLLTPSIMGFDALYLFIQTVIVMFS
                     GQKKIAMMSGYTNFFVSVFVMIICSFGLFLILFRGREKNIYFLLLTGTVIGSLFGGLS
                     SFMQVLLDPHEFSILQNKMFASFNNVNQNLLQVSAILVAVTMLLTVRDNPYLDVVSLG
                     QEHAVNLGVNYRRVVLKNLMVTAVLVSISTALVGPITFLGILVVSITRKVTTTYKHTA
                     RIAMAFLTSSTLLVSAMYLTERVFKFSAPISVLINFAGGIYFIYLILKERKA"
     gene            complement(3271..>3485)
                     /locus_tag="PLI11_06700"
     CDS             complement(3271..>3485)
                     /locus_tag="PLI11_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPZ52589.1"
                     /translation="IVSLYKGDNLRNCLFDTAALGALFLLVCDIIGRVVIYPYEVSIS
                     VVVSILGSIIFLILIFARKGKTIKNA"
BASE COUNT         1011 a          728 c          624 g         1122 t
ORIGIN      
        1 tctgcgttgt cgctgagttt tggctcaaca gaaatgtctc ccaaagaaac aataccgaca
       61 agacgatcgt ttgaaacaac aggaagtctt cttacttgtt tcacactcat aatccttgca
      121 gcctcatgaa cgtctatttc aggagtagct gtgacaggat ttagtgtcat aatatccttt
      181 actgttagag agttaacgtc ttttccaaca gctattgatc gcaatacaat atctctgtcg
      241 gtgagtattc caataacttt ctggtcgtcc atgcaaacag ggacagcacc aattccatgc
      301 tgtttcatta gttcagcagc tttttttact gattcatttt ggtcaacact tataacattc
      361 gttgtcatga tttcatttaa cttcattctc aacacctccg atattatgtt aacccttaat
      421 ttgtttttca tagctagcat attttgatat tttgacaaga tgctcggacc actttacttg
      481 ctcaaaaata aaaccggaca tgtagcccgg tttgccaaag ttcagatgtt tttatcaaaa
      541 cgctttttgg atgtcactca acattactgc cattgtattc agtccaccgc tcgctaggta
      601 ccaagcatca ggatcaaggt aaacaatcat attttctttg gctgcgtttg tcgatttgac
      661 caaatcgttg ttcagagtat cgctggcttt aacggttcct ccaaccacaa cagtgcggtc
      721 aactacaaag ataatatcag gattaacctg tgcaatatac tcaaagcttg cggattgtcc
      781 gtgagttgaa gtttcaattg tgtcgtcggc agtttttatt ttcagcacat catgaatcag
      841 accaaaacgt gaacctttgc cataaacact aatattgcca tcgttggtaa gaagagtaag
      901 tgctttcttt tctgaattat cagcttttaa tttcaaatat tcaatcgtgg cgtcaatttc
      961 atttatatga tcgtcggctt tgttgctcag accgagtatt gaagctatgt tctgagtatt
     1021 ttgcttgaag tcttccataa atgcttctgg tctcaaatca acaaacatcg ttggggctat
     1081 ttctgttagt tgttcatagt agtcttgctg tcttgttcca attataataa cgtcgggttc
     1141 gaaattgaat atggcttcca agtcgggctc aaaaagtgta cccatatcct tggcgttttt
     1201 atacttggat aaactagtgg gtatgttgct ggttggaaca cccagatcaa cctcagcacc
     1261 aagtgcatct attgtatcta aagcgcccat atcgaataca acaacttttc tggcgacctt
     1321 gtttatagta aattcacctt tgttatgtat tactttgatt tgtgtatttc cgttattatc
     1381 accatcatcg gtctctacag gtgggttgaa aagataaccg catcctgtca agacaacaag
     1441 agtcagcgac attaccatca atatcataaa taactttttc atattaaaat ttcctcctca
     1501 attttgtact ctgtttgaat catgcaatat gcatagctag ttgtaataca aacatattgg
     1561 ttttccattc acactctgca catctatatc catgccgtag atttctttca gtacctcttt
     1621 cgtgataatt tcatcagtat atccttgact caccaccttg ccgtccttca tggctattat
     1681 gtagtctgag taatatgcga tgaagtttat gtcatggatt acaaccatga tggtcttgtt
     1741 catctcatca acaagtttgc ggagcccttt cattatttgt actgagtgcc tcatatccag
     1801 gttgttaagt ggctcatcaa gaaaaacata ctccgtatcc tgggctatta tcatagctat
     1861 gtatgccatt tgcctttgac ctccgctcag ctcgtcgata ttgcggtgct ggatattatc
     1921 caaacttaag aaagaaagtg ctttgtcgat aatctcgttg tctttctttg tcaaacgacc
     1981 ttttgaatat gggaaccttc caaaacttat aagttctctc actgttagcc tgatatttag
     2041 gtggtttgac tgctttaata tggaaatacg tttagacagc tcgttattat tccattgctg
     2101 gagttccttg ttgtctacat aaacacatcc ttcagttttg ggaacaatcc tgctcataag
     2161 tgaaatcaga gtgcttttgc cagccccatt tgcacctata aatgcaatca tctttccttt
     2221 tggaaggttt agacttacat tgttcaaaac aaccttttcg ccgtaagatt taactaaatt
     2281 ctcagatcta atcatgcttt tctctccttt agtattaagt aaataaagta aattcctcct
     2341 gcaaaattaa taaggacact aatcggtgcg ctgaacttga aaactctttc ggtaagatac
     2401 atggctgata ccaaaagagt tgaacttgtt agaaacgcca ttgcaatcct tgctgtgtgc
     2461 ttgtatgtag tggtcacctt tctggtgatg ctgacaacaa gtatgcctaa aaaggtaatt
     2521 ggtcctacaa gagctgttga tatagaaacc agcaccgctg tgaccatcaa atttttaagt
     2581 accaccctgc gatagttaac tcccaagttg accgcatgct cttgacctag tgataccaca
     2641 tcaaggtaag ggttatccct gactgtcaga agcattgtta cagcaactag aatagcactt
     2701 acttggagca aattttggtt tacgttatta aagcttgcaa acatcttgtt ttgcagtata
     2761 gagaattcat gaggatccaa tagaacctgc ataaaagatg acaagccgcc aaacaaggag
     2821 ccaatgactg taccggtaag aagaaggaag taaatgtttt tctcccttcc tcggaacaat
     2881 attagaaaca agccaaacga gcaaataatc ataacaaaaa cggagacaaa gaaatttgtg
     2941 tatccgctca tcatggcaat cttcttctga cctgaaaaca tgacaataac tgtttggata
     3001 aacaggtaca aagcatcaaa acccatgatg gatggcgtca gcagtctgtt attggttatt
     3061 gtttggaagg ttaccgatga ataactaaca cagtatccca ccaacaagac cgctgcaatc
     3121 ttgctaagac gcctgggaaa gaagaaatat gcattctttg ttgtcaggcc ttggaacaaa
     3181 aacaagctgc ctgtcaagag aagcacgaat gtaaaaaacg ctgctattgc aattgttttc
     3241 ttgtgttttt ttattacttc catggcatac ctaggcattt ttgatcgtct ttcctttcct
     3301 ggcaaatatt aatatcaaga agataatgct gcccaaaata ctgactacca cgcttatgga
     3361 tacttcatag gggtagatga ccacacgacc aattatgtcg catacaagca aaaacaaggc
     3421 tcccaaggct gctgtgtcaa aaaggcaatt tctcaaatta tcacctttat aaagggaaac
     3481 aatgt
//
LOCUS       DAONVY010000227         3484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18707_length_3484_cov_3.286381, whole genome shotgun sequence.
ACCESSION   DAONVY010000227 DAONVY010000000
VERSION     DAONVY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18707_length_3484_cov_3.286381"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="rsmA"
                     /locus_tag="PLI11_06705"
     CDS             <1..635
                     /gene="rsmA"
                     /locus_tag="PLI11_06705"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPZ52590.1"
                     /translation="PVLEHLKEDYENIEIVNEDVLKLDINKEIIEKYCSDKNVKVVAN
                     LPYYITTPIIMKFLEENVMNLESMTFLVQKEVAERICAKPGGKEYGMLTVAVNYFAYP
                     KSLFKVSPSVFIPKPNVDSSVIRFDVRKERPFHLKDQDFFFKVLKASFAQRRKTLANS
                     LSNAPYLNVTREDVCKALEEMGKGISIRGEVLSGEEFGTLSNILFDIHTK"
     gene            758..2515
                     /locus_tag="PLI11_06710"
     CDS             758..2515
                     /locus_tag="PLI11_06710"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPZ52591.1"
                     /translation="MYNNKYVNEWVEQMAQLTQPDEIVWIDGSEEEKDRLTKQALSTG
                     ELVELNQETYPGCYYHRTAENDVARVEHLTFICTSKKENAGPTNNWMEKSYAYARLGN
                     LFNGSMKGRTMYVIPYLMGPPGSEFSKVGIEITDSIYVVLNMRIMTRIGKIAMDFLGD
                     SNDFVKGLHSKGELDPEKRFICHFPEDNAIWSIGSGYGGNVLLGKKCFALRIASAMGR
                     REGWMAEHMLILGIEDPNGKVEYIAAAFPSACGKTNLAMLIPPEKYKKAGYKVWTVGD
                     DIAWLRIGKDGRLWAINPEAGFFGVAPGTSMKSNPNALLTTKKNTIFTNVALTENKEV
                     WWEGMGVPAPEKALDWRGNPWTPDMDTKAAHPNSRFTAPAKQCPCISDKFEDPNGVPI
                     SAIVFGGRRAKLAPLVYQSFDWEHGVYVGATMASETTAAATGKVGVVRRDPMAMLPFC
                     GYNMGEYFQHWIEMGRKIPNPPKIFNVNWFRLDDEGKFIWPGFGDNLRVLLWILDRCN
                     DKADAVKTPIGYLPNPGDIDLEGTDVDLDTLKSILDVNKEDWIEELKSQEEHLGKFDK
                     LPVEVRNQFEAQKERINNL"
     gene            2622..3341
                     /locus_tag="PLI11_06715"
     CDS             2622..3341
                     /locus_tag="PLI11_06715"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPZ52592.1"
                     /translation="MQDKDDFMKDMDRMYKKLKRMRRRIKKRELVGLIPLFITLILAG
                     VFIHTLSMRFDSESFAEFNESYIDTIIITLGFYAFKALTLFVISPVLIYGFVGLLFSM
                     PVAMIVLVAGLFLELSINFIMGKFLGRVYVERLVLRIDGEGRLLERLKNIHNSRMNIF
                     LMRFIGVPPIGITSVYLSAMGLEFKKYLIYSMAGLLPKAILITLAGSAVRTPDSPLFV
                     VSVVCLLVLGIVFYIYGKKNK"
BASE COUNT         1069 a          613 c          820 g          982 t
ORIGIN      
        1 tcccggtgct ggagcatctc aaggaggact atgaaaacat tgagattgtc aacgaggatg
       61 ttttgaagct tgacataaac aaggaaatta ttgaaaagta ttgctctgat aaaaatgtaa
      121 aggttgttgc caaccttcca tattatatta caacaccaat aatcatgaaa tttcttgagg
      181 agaatgtaat gaatcttgag tccatgacgt ttttggttca aaaggaagtg gcggagagaa
      241 tttgtgcaaa acccggaggg aaggaatacg gcatgcttac tgttgctgtc aactactttg
      301 cctatcccaa aagtcttttt aaagtttcac cgtctgtttt cataccaaaa cccaatgtgg
      361 attcaagcgt gatacggttt gacgtaagga aagaaagacc atttcacctc aaggaccagg
      421 atttcttctt caaggtgctg aaggcttctt ttgcccagag aaggaaaacc cttgcaaaca
      481 gtctttccaa tgctccctat ttaaatgtta caagagaaga cgtttgcaag gctttggaag
      541 agatgggcaa gggtatttcc ataaggggag aggttctttc gggtgaagaa tttggtactc
      601 tttcgaatat tttgttcgat attcacacaa aataaaaaag ttaataaaat gttataagtt
      661 attttaattc cttattattg tattttattg ctaataagat ataataaaat aggcattttt
      721 atttataaaa ttttaatgct cagaaagagg tagaaatatg tataacaaca aatatgtaaa
      781 tgaatgggta gagcagatgg ctcaattgac acaacccgat gaaattgtgt ggatcgatgg
      841 ctccgaagaa gaaaaagaca gacttacaaa acaggctttg tctacaggag agcttgtcga
      901 acttaaccaa gaaacatatc ccggatgcta ttatcataga actgcggaaa atgatgttgc
      961 ccgcgtggaa catcttactt tcatttgtac ctcaaaaaag gaaaatgcag gtcctactaa
     1021 caattggatg gagaaaagct acgcttatgc aagactaggc aatttgttca atggttcaat
     1081 gaagggtagg acaatgtatg ttatcccata cttgatggga cctccaggtt ctgaattcag
     1141 caaagttggt atcgagatta cagacagtat ttatgttgtt ttgaatatga gaataatgac
     1201 tcgcatagga aaaattgcaa tggatttcct tggcgattcc aatgattttg ttaaaggact
     1261 ccattccaag ggagagttgg atccagagaa gagattcata tgccacttcc cagaggataa
     1321 tgcaatctgg tccataggtt ccggctacgg cggaaatgtt ctcctgggta agaagtgctt
     1381 tgcacttcgt atagccagcg ctatgggcag aagagaagga tggatggcag agcacatgct
     1441 tattcttgga atagaggatc caaatggaaa ggtagagtat attgcagctg ctttcccaag
     1501 cgcatgcggt aaaacaaacc ttgcgatgtt gattccacct gagaagtaca agaaggcagg
     1561 ttacaaagtc tggacagtag gtgacgatat tgcgtggctc agaattggca aggacggaag
     1621 actctgggca atcaacccgg aagcaggttt ctttggcgtt gctcctggaa ccagcatgaa
     1681 gtccaatcca aatgcgcttc tgaccacaaa aaagaataca atatttacaa acgttgcatt
     1741 gacggaaaac aaggaagtat ggtgggaagg catgggtgtt cccgcaccag aaaaagcgct
     1801 tgattggaga ggcaatccat ggacgccgga catggatacc aaagctgcac atccaaactc
     1861 aagatttaca gcacctgcaa aacagtgccc gtgcatttct gacaagttcg aggatccaaa
     1921 cggtgtgccc atttcggcta tcgtatttgg cggacgtcgt gcaaaacttg ctccgctcgt
     1981 ataccagtcc tttgattggg agcatggtgt atacgtaggt gcaaccatgg catccgagac
     2041 aaccgctgct gcaacaggca aggttggagt tgttagaaga gatcctatgg caatgctgcc
     2101 attctgcgga tacaatatgg gcgagtactt ccagcattgg attgaaatgg gcaggaagat
     2161 tccaaatccg ccaaagattt tcaatgttaa ctggtttaga cttgatgacg aaggcaagtt
     2221 catctggcct ggattcggtg acaacctcag agtactactc tggatactgg acagatgcaa
     2281 tgacaaggcg gacgctgtca agactcctat aggatatctg ccaaacccag gagatatcga
     2341 tctggaaggc actgatgttg atcttgatac acttaagagc attcttgacg tcaacaagga
     2401 agactggata gaggagctca agagtcagga agagcatctt ggcaagttcg acaagcttcc
     2461 tgttgaagtt agaaatcaat ttgaagctca aaaagaaaga atcaacaacc tgtaacaata
     2521 aattaagttt taaagccaca gacaatgtct gtggctttta tcatataatt cttgtcctgt
     2581 catatattag ttattgatta ctttcaagga cgggtgtgcg aatgcaggac aaagacgact
     2641 tcatgaaaga tatggacagg atgtacaaga aactgaaaag gatgagaaga agaataaaga
     2701 agcgggagct tgtgggttta attccacttt ttattactct tatccttgcg ggtgtgttca
     2761 tccatacatt gagcatgagg tttgattctg aatccttcgc agagttcaac gaaagttata
     2821 ttgatacgat aataattacg cttggattct atgcattcaa agcgctgact ttgtttgtta
     2881 tctcaccagt attgatttat gggtttgtag gtttgctgtt cagcatgcct gttgcgatga
     2941 ttgtacttgt tgccgggttg tttttggaac tttcaataaa tttcataatg ggtaagtttt
     3001 taggcagggt atatgttgaa aggcttgtac tgcgcattga tggcgagggt aggttgttgg
     3061 aaaggcttaa aaacattcac aacagtcgaa tgaacatatt tctaatgcgg tttattggag
     3121 tgcctccaat aggtataacg agtgtgtatt tgtctgccat gggtctggaa ttcaaaaagt
     3181 atttgattta ttcaatggcg ggacttcttc caaaagccat attaataacc ttggcagggt
     3241 ctgctgtaag gacgccggac tctcccttgt ttgttgtatc cgttgtctgt ttgttggtgc
     3301 tgggtattgt gttttacata tatggcaaga agaataaata acaagaaaaa gagctgtttt
     3361 taacagctct taaaagaagt tataccttta cttttctctt tgcaataaaa aggacgcttg
     3421 ccaacacgaa gaatatgatg gcttgagcga atgctgcaga atcgccggtt ggaacatttg
     3481 gttc
//
LOCUS       DAONVY010000228         3481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18725_length_3481_cov_4.211033, whole genome shotgun sequence.
ACCESSION   DAONVY010000228 DAONVY010000000
VERSION     DAONVY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18725_length_3481_cov_4.211033"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="PLI11_06720"
     CDS             <1..1617
                     /locus_tag="PLI11_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5724 domain-containing protein"
                     /protein_id="HPZ52593.1"
                     /translation="KAGAFDSRWFREALSEVGEKNFALLYKAAKYIAGGNLHKRSQLF
                     ADSVRGKLDKSEVFEKVAKSRNKDYLLGYTLMPYESQEELLERYMFVENFRKESRQFG
                     AMRRQSEAQAYDIAIQNLARIGGYEDTNRFVWQMESLKLDSVRHWFESVKVDELILTI
                     EFDEEGIASMKCTKDDKVLKSVPAAYKNHPVVAEGLEVVKSLKEQQKRARKTLERAMV
                     HRDAFTKRELELLARNPVLGPSTKKLLFVSSGFVGFFADGFLVDFNGSMTQVDENIYI
                     AHPYDLMTSKTWHDWQKHFYTERIKQPFKQVFREYYCPNVDELKEKTISRRYAGHQLQ
                     PQKAARLAEERLWNTSEGLFKVYFKEHIMVSLNFESGWFSPMDIEVPTIHSVEFFNTD
                     NWNVMNITEVPPVLFSEAMRDIDLLVSAAHYGSVDPEASLSTIEMRTALINVLLPLLK
                     IDNVTINKSHALIKGEFGEYTVHLGSGVAHMQAKGMMPILPVHSSHRGRVFLPFADDD
                     LKTAEVLTKIVMLAEDKKIKDPVILGFIRS"
     gene            1648..1833
                     /locus_tag="PLI11_06725"
     CDS             1648..1833
                     /locus_tag="PLI11_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52594.1"
                     /translation="MDKVLEEAISLLDGGGFHYAVYGGYAIELFLDRNIRKHADVDIS
                     VYWHERDRIIQYMQHLG"
     gene            1943..2095
                     /locus_tag="PLI11_06730"
     CDS             1943..2095
                     /locus_tag="PLI11_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HPZ52595.1"
                     /translation="MCSRSRIGLPLAKDANHGLGTQSIRHVVEKLHGNCQFAVKDYLF
                     VLRVVL"
     gene            2154..2999
                     /locus_tag="PLI11_06735"
     CDS             2154..2999
                     /locus_tag="PLI11_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HPZ52596.1"
                     /translation="MKIACNYYLETEELFDEGRIHLDYFKYPALGFQMEIMKDLDAFE
                     GLCSRIAQKRPILLHGLYPAPHDLSSATLKEDFDDRIADRLVETTKTPGLSFHPALSK
                     KPERVPFGAFFETIVDNARYIKEKYEYLEFVLIENCDNVMWGELIKPDVFSRLVHESG
                     CGLLLDISHAFCAAKWLDMPFYEYMEKLPLDKTVEVHINGWIEIPGAVMCHTMIHEEG
                     YKALEFVLDRCRPEIVTVEYGRDNDKIGAGIPLVLHTDINEKIKEEIEIQMHRVWEIV
                     KSRAN"
     gene            3137..>3481
                     /locus_tag="PLI11_06740"
     CDS             3137..>3481
                     /locus_tag="PLI11_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ52597.1"
                     /translation="MVYDVAIIGAGVTGAMTARELSRYELSVCVIEKESDVAMGASKA
                     NSGIVHAGFDAKSGTLKARFNVEGSLMMEKITKELGVKYKRNGSMVLAFSEEEKEILV
                     ELYERGIRNGVSS"
BASE COUNT         1061 a          555 c          898 g          967 t
ORIGIN      
        1 aaggctggag cttttgattc caggtggttt agggaagctc taagcgaagt gggagagaaa
       61 aactttgccc tgttgtacaa ggctgccaaa tatattgcag gagggaacct gcacaagcgt
      121 tctcaactgt ttgcggatag tgtgagagga aagcttgata aaagtgaagt gtttgaaaag
      181 gtagcaaaat caaggaacaa ggattacctg cttggttata cactgatgcc gtatgaaagt
      241 caggaagaac tgctggaaag atatatgttt gtggagaatt tcagaaagga aagcaggcag
      301 tttggtgcaa tgcgaaggca aagcgaggct caagcttatg atattgccat ccagaatcta
      361 gctcgcattg gtggttatga ggatacaaac aggtttgtct ggcagatgga atccttgaaa
      421 ctcgatagtg taaggcattg gtttgagtct gttaaagtgg atgagctaat attaacaatt
      481 gagtttgatg aagaaggcat tgcctcaatg aagtgtacaa aggatgacaa ggtattaaag
      541 tcagtgcctg ctgcatacaa aaaccatccg gttgtagcgg aagggctgga ggttgtcaag
      601 tctttgaaag agcagcaaaa aagggctagg aagacattgg aaagagccat ggtgcacagg
      661 gatgcgttta caaaaagaga gcttgaattg cttgcaagga atcctgtcct tggtccttca
      721 acaaaaaagc ttctctttgt gagcagtggc tttgtggggt tcttcgcaga tggtttcttg
      781 gttgacttta acggcagcat gactcaggtt gatgaaaaca tatacattgc ccatccttat
      841 gatttaatga cgtcaaaaac atggcatgac tggcagaagc atttttatac tgagagaata
      901 aagcagcctt tcaagcaggt gtttcgtgag tattattgcc cgaatgtgga tgagcttaag
      961 gaaaaaacca tatccaggcg atatgcagga catcagcttc agcctcaaaa ggctgcaaga
     1021 ctagcagaag agcgcctttg gaataccagc gaaggtttgt tcaaagtata cttcaaagag
     1081 catattatgg tttcactgaa ctttgagtcc ggttggtttt cgcctatgga tattgaggtg
     1141 ccgaccatac attcagtaga gtttttcaat actgacaatt ggaacgttat gaatatcacg
     1201 gaagtaccgc ctgtactttt ctcagaagcc atgagggata ttgatttgct tgtcagtgct
     1261 gcccattacg gaagtgtgga cccggaagca agtctgtcca ctattgaaat gcgcactgcc
     1321 cttatcaacg tgctgcttcc acttctcaag attgacaatg ttacaataaa taaatcccat
     1381 gcgctaatca aaggagagtt tggcgaatat actgttcacc tgggaagtgg tgttgctcat
     1441 atgcaggcaa agggaatgat gcctattctt ccagtgcatt cctctcacag aggaagagta
     1501 tttcttcctt ttgccgatga tgatctcaaa actgctgagg tgcttacaaa gatagtaatg
     1561 ttggcagaag acaagaagat aaaggatccg gttatacttg ggtttattcg ttcatagaag
     1621 agtattgata gaaagagaga gaggaaaatg gacaaagtgt tggaagaagc tattagtttg
     1681 cttgatggtg gtggttttca ttatgctgtt tacggagggt atgcaattga gcttttcctt
     1741 gacagaaaca tacgcaagca tgcagacgtt gacatctcgg tatattggca tgaaagggat
     1801 cggattattc agtacatgca gcatttgggt tgacgtgttt ttgagcctat tggcaataat
     1861 ctggtacacc acattgaaga cgtaaacgac cagatgatgg tgaaaagaaa cctctttagc
     1921 ttcttttgtc cataaagaac ccttgtgttc aagaagtaga attggactgc ctttggcaaa
     1981 agacgccaac cacggtcttg gaacgcaaag cataaggcat gtggttgaga agctgcatgg
     2041 aaattgtcag tttgctgtca aggattattt gtttgttctc agggttgttc tttagaattt
     2101 gtaatcggag aagctgtatt gtataatatt ggcaaaaaca gtaggagatt aagatgaaga
     2161 ttgcatgcaa ctattatttg gaaacagaag aactatttga cgaaggaaga atccatctcg
     2221 attatttcaa atatcctgct cttggctttc aaatggagat tatgaaggac ctggatgctt
     2281 ttgaaggtct ttgcagtcgt atagctcaaa aaagaccgat tcttctgcat ggcctttatc
     2341 ctgcaccgca tgatctgtct tctgccacac ttaaggaaga ctttgatgat agaattgcag
     2401 acaggcttgt tgaaacaaca aaaaccccgg gcttgtcttt tcatcctgca ttgtcaaaaa
     2461 aacctgaacg tgtgcctttt ggtgcttttt ttgaaacaat tgtagataat gcaaggtaca
     2521 taaaagaaaa gtacgagtat ttggagtttg tcctgattga aaactgcgac aatgtaatgt
     2581 ggggtgaact cataaagccg gatgtatttt caaggcttgt acatgaaagc ggatgcggcc
     2641 ttcttttgga tataagtcat gcgttttgtg cggcaaaatg gctcgatatg cctttttacg
     2701 agtacatgga aaagcttcct cttgataaaa ccgtggaagt gcatataaat gggtggattg
     2761 aaatacctgg tgctgttatg tgccatacga tgatacatga agaaggctac aaggctcttg
     2821 agtttgtact tgataggtgc agaccggaaa ttgtcactgt ggaatatggg agggacaatg
     2881 acaagatagg agccggaatc ccgctggttc tgcatacaga tattaatgag aaaattaaag
     2941 aagaaataga gattcagatg cacagggtgt gggaaatcgt gaaaagtaga gcgaattgac
     3001 agaaagagag aatacgggta taatttaagt ataaataaat attgcatgtg tagagatttg
     3061 gagacacacg taaaaatgaa ggcgaggcct ttgttttcgt gtgtcttttt tttcatgtca
     3121 tgaggaaggt gtacatatgg tttatgatgt tgcaataatt ggtgctggtg ttactggtgc
     3181 aatgacagca agggaattgt ctagatatga actgtcggta tgtgtaattg aaaaggaaag
     3241 cgatgtggca atgggggcat caaaagccaa cagcggcata gtgcatgcag ggtttgatgc
     3301 aaaaagcggt actttgaaag ccagattcaa tgttgaaggc tcgctgatga tggagaagat
     3361 aacaaaggaa ctgggtgtga agtacaagag aaacggctcg atggttcttg cttttagcga
     3421 agaggagaag gaaattcttg tagagcttta tgaaagaggt attagaaacg gagtaagcag
     3481 c
//
LOCUS       DAONVY010000229         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18777_length_3474_cov_2.905820, whole genome shotgun sequence.
ACCESSION   DAONVY010000229 DAONVY010000000
VERSION     DAONVY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18777_length_3474_cov_2.905820"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLI11_06745"
     CDS             <1..211
                     /locus_tag="PLI11_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPZ52598.1"
                     /translation="SSEAIKRQMASGIDLIVHLKRNHLMERKVSEIAEVCGYEDGEVV
                     LNTLFRDGKKVSGLKVKGYKVEGTE"
     gene            208..963
                     /locus_tag="PLI11_06750"
     CDS             208..963
                     /locus_tag="PLI11_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPZ52599.1"
                     /translation="MNKTRLVDYKKYVFSFKDWCVFAFVLTAISVMFGLVLFESLKIG
                     IVFSIISSPFVFARLKKYFMRKRLEELERQFCDALQLMSASLSAGLSFTSCISEIASG
                     DLSVDGNLRLVQKEFANMQRLIALNISPEEAVEAFAKRTGSRDIQNFSNALYESLQAG
                     GNLVQLVRNSASALRVKYDSEDEIRSLLNLPRFNHRIMMVMPFVLILMVKAIAPGYVE
                     PLYTEGARWILFLVAFLIILSWYIGERISDIKF"
     gene            975..1700
                     /locus_tag="PLI11_06755"
     CDS             975..1700
                     /locus_tag="PLI11_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPZ52600.1"
                     /translation="MGVVESIKKYYEGVCEKYVSLYGKKRGSHEFRKAVNKAVVGYVA
                     SFILFPLTYVYGKSLFLSVVVFLVLIISFILLPNSLIESKMKDLRRSFSLNMPDFLER
                     VAILLDSGQNLWASIEKAAMVNDDEFSLEGEISRTMKEINECRGSEKDPCDAFSDMAK
                     RLESSHVSSFVSLIVQNSRKGQAELVEIIRMQAAIYRNDRKNVARKLGEEATTLMLIP
                     TTIVFIGILIMLLSPAFLVIYSI"
     gene            1714..1905
                     /locus_tag="PLI11_06760"
     CDS             1714..1905
                     /locus_tag="PLI11_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HPZ52601.1"
                     /translation="MFLKAYFAIRKILDKRGVGTVEILIILAVLVALALIFRQFIIEV
                     AERIFDKIRENADKAIEGL"
     gene            1908..3011
                     /locus_tag="PLI11_06765"
     CDS             1908..3011
                     /locus_tag="PLI11_06765"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPZ52602.1"
                     /translation="MESVVLYEERKKINISHDRGGVRITVSTPVSPSEIKEKHAVVAD
                     VADNVYIPSEITQEAKAYSFYIRKRQTVKDFLIQNECTISDLVSLIKSVNEILQLADR
                     LHLNRYDFIFDYSCIFTGQTLDTAEFVYAPGADLDREQNSISNMLLIASLHVACDENS
                     QEAVALKEATGIIIEWEASGTSPFPLEEVLAVLDVEEKNEGIFFTTWKPFLCFQFIML
                     LVFIFIVSLVPFRENGVFIWIAFVLLTVCVDYLLIPGKKKNKPEFKLLRRKVLRGMGG
                     LKGKIIYVDWDTIKIGRDAEWADVVISNIFVSRKHAILYFKDNILYVKDLSSRNGTFL
                     NGVRLEANVEVVVEAGSTVVFGHDSVIIKYCYF"
     gene            3098..>3474
                     /locus_tag="PLI11_06770"
     CDS             3098..>3474
                     /locus_tag="PLI11_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPZ52603.1"
                     /translation="MGFFRQNIAVDLGTATILVSVENKGVVIKEPSIVAIKDASNKIL
                     AIGEEARSLMGRTPFDISTVKPIQNGVISSYYTTMRMLKYYYKKALRNPLKKILKPDV
                     IICVPSQTTDVEKRAVKKASQDAG"
BASE COUNT         1036 a          517 c          823 g         1098 t
ORIGIN      
        1 aagttccgag gcaataaaga ggcagatggc atcaggtatt gatttgatcg ttcatctaaa
       61 gagaaatcat ttgatggaga ggaaagtttc cgaaattgca gaagtttgcg gttacgagga
      121 tggagaggtt gttttaaaca cattgttcag agatggtaaa aaggtatctg gtctgaaggt
      181 aaaaggatac aaggtggagg gtactgaatg aacaagacaa ggcttgtaga ttacaaaaag
      241 tacgtatttt cttttaagga ctggtgtgtt tttgcgtttg tgttgaccgc catttccgtt
      301 atgtttggcc ttgttttgtt tgaaagttta aagattggca ttgtcttttc cattatttcc
      361 tctccttttg tttttgcaag attgaaaaaa tactttatga gaaaaaggct tgaagaattg
      421 gaaagacagt tttgtgatgc gttgcagctt atgtctgctt ctctttccgc aggcttgagt
      481 tttacaagct gcataagtga aattgcgagt ggcgatttgt ctgttgacgg caacctaagg
      541 cttgtgcaaa aggagttcgc aaatatgcaa agactcattg ctcttaacat atcgcctgaa
      601 gaagctgtgg aggcttttgc aaagaggacc ggcagcaggg atatacaaaa cttttcaaac
      661 gcactttatg aaagtctgca ggctggaggc aatcttgtgc aacttgtccg taattcagcc
      721 agtgcattaa gggttaagta tgactcggaa gatgagataa ggagcctttt gaatcttccc
      781 cgattcaatc acaggataat gatggtcatg ccgtttgttt taatacttat ggtaaaggct
      841 atagcgccag gatatgtgga acctttatac acggaaggag caaggtggat tttgtttctt
      901 gtagcatttc taattatttt gtcttggtat ataggagaga gaatatcaga tatcaagttc
      961 tgaggatgat tgtaatgggt gttgtggaga gtataaaaaa gtattatgaa ggggtttgtg
     1021 aaaagtatgt aagtttatat gggaaaaaaa gaggcagcca cgagttcagg aaggctgtca
     1081 ataaagcagt tgttggttat gttgcttctt ttatactttt tccattgact tatgtttatg
     1141 gaaaatctct ttttttgagc gtagttgtgt ttcttgtttt aatcatctct ttcatactct
     1201 tgcccaactc gcttattgaa agcaaaatga aggatttgag aagatctttc agtttaaata
     1261 tgccggattt tcttgaaagg gtggccattc ttctcgacag cggccagaac ctttgggcat
     1321 ccattgagaa ggcggccatg gttaatgacg atgagtttag tctggaaggt gagatatcca
     1381 ggacaatgaa ggagataaat gaatgcagag gttctgaaaa ggatccttgc gatgcgtttt
     1441 ctgacatggc aaaacgcctt gaatcatcgc atgtcagctc ttttgtttca ttaattgttc
     1501 agaattcaag aaaggggcaa gcagaactgg ttgaaattat tcgaatgcag gctgcaatat
     1561 acagaaatga cagaaagaat gttgccagga aactgggaga ggaggcgacc acgctgatgt
     1621 tgataccgac taccattgtt tttattggca ttctaattat gcttctttca cctgcatttt
     1681 tagtaatata ttcgatttag gggggattct gcaatgtttc tgaaagcata ctttgccatc
     1741 aggaagattc ttgacaaaag aggtgtgggc actgtcgaga tattgattat acttgcggta
     1801 cttgttgccc ttgcgcttat ttttagacag tttattattg aagttgcgga aagaatattt
     1861 gataaaattc gtgaaaatgc cgacaaggcc atagaagggc tgtgagtatg gaaagtgtag
     1921 ttctgtatga agaaagaaag aagataaata ttagtcatga cagaggtggt gtgagaatta
     1981 cggtaagtac acccgtgtca ccgtctgaaa taaaggaaaa gcatgcagtt gtggctgatg
     2041 tggcagataa tgtatacatt ccatccgaga taacccagga agccaaggct tattcctttt
     2101 acataagaaa gaggcagacg gtaaaggatt ttcttattca aaacgaatgc acaatttctg
     2161 atcttgtgag tttgataaag tccgtcaatg aaatccttca actcgcagac agactgcatt
     2221 taaaccgata tgactttatt ttcgactact cttgtatttt tactggtcag acccttgata
     2281 ctgcggaatt tgtttacgca ccgggtgctg atttggatag ggagcaaaac agcatttcaa
     2341 acatgctgct gattgcctcc ttgcatgttg cttgcgatga aaactcacaa gaggcagtgg
     2401 cattgaaaga agcaacaggc attattattg aatgggaagc atcaggcact tctccttttc
     2461 cattggaaga ggtattggcg gttcttgatg ttgaagagaa aaacgaagga atcttcttta
     2521 ctacctggaa accattctta tgctttcaat tcataatgct gcttgtcttt atttttatcg
     2581 tatcccttgt tcctttcaga gagaacggag ttttcatttg gattgcattt gttttgttga
     2641 cagtatgtgt tgattatcta ttgattccag gaaaaaagaa aaacaagcct gaattcaagt
     2701 tgttaagaag aaaagtttta agaggcatgg ggggtttaaa aggaaagata atttatgtgg
     2761 actgggatac aataaagatt ggaagggatg cagagtgggc ggatgttgtt atttcaaaca
     2821 tctttgtcag tagaaagcat gcaattcttt attttaaaga caatattctg tatgtgaagg
     2881 atctgtcgtc aagaaatggc acttttctta atggagtgag gcttgaagca aatgttgagg
     2941 tggtagttga agcaggaagt acggttgtat ttggtcatga tagtgttata ataaagtatt
     3001 gttattttta agctttctat tttttagtac ttgaataatt aaattttata tgttatgatt
     3061 tcaaaggtta tttaaattta tttcaggggg aaatttagtg ggttttttta gacaaaacat
     3121 tgctgttgac ctgggtactg ctactattct tgtaagtgtc gaaaacaagg gcgtagttat
     3181 taaagaacca tcaatagtgg caattaaaga tgcaagcaac aagattctgg caataggtga
     3241 agaggcaagg agcctgatgg gtagaactcc ttttgatatt tcgactgtaa agccgattca
     3301 aaatggtgtt atatccagct attataccac tatgcgcatg cttaaatatt actacaaaaa
     3361 agctctcaga aacccgctta agaaaatcct taaacccgat gtgattatat gtgttccaag
     3421 tcaaactact gacgttgaaa agagagcggt caagaaggcg tcccaagatg ctgg
//
LOCUS       DAONVY010000230         3470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18810_length_3470_cov_3.368960, whole genome shotgun sequence.
ACCESSION   DAONVY010000230 DAONVY010000000
VERSION     DAONVY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18810_length_3470_cov_3.368960"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1306)
                     /gene="dnaJ"
                     /locus_tag="PLI11_06775"
     CDS             complement(185..1306)
                     /gene="dnaJ"
                     /locus_tag="PLI11_06775"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPZ52604.1"
                     /translation="MSTKRDYYEVLGVSRDASTDEIKKAYRKLAKKYHPDVNPDNKEA
                     EAKFKEATEAYEVLSDSEKRAQYDRFGHAAFDPNSAAGGGFGGFDFGGFGFGGLDDIF
                     DFFGTGFGSRQSRGPVKGADIRTTMTITFEEAAFGTEKDVSVNKYVSCSTCEGTGSKS
                     KSTSSCTNCGGSGQVRVKQATPFGSFVNVRTCDKCGGTGKTVTDPCTVCSGNGKVRKN
                     VTIKVKVPAGIDHGQAISMSGQGEPGSKGGPPGDLIISIRVQRHKLLRREGFDVYLDV
                     TIDMIQAALGDVIVVDTLDGKVELTIPEGTQPGVSFKLKGKGIPKLHSSGRGDQIVRV
                     SVAIPKKLNETQKDLLRQLQASITGNSSNGRRSFFGRKK"
     gene            complement(1419..3272)
                     /gene="dnaK"
                     /locus_tag="PLI11_06780"
     CDS             complement(1419..3272)
                     /gene="dnaK"
                     /locus_tag="PLI11_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPZ52605.1"
                     /translation="MSKVIGIDLGTTNSCVAVIEGGEPVVIPNPEGSRTTPSVVAFTK
                     AIEGKPAERLVGAAAKRQAITNPDRTIMSIKRDMGTDRRITIDEKRYTPPEISAMILQ
                     KLKADAEAYLGEKVSKAVITVPAYFSDSQRQATKDAGRIAGLEVLRIINEPTAAALAY
                     GLDKENEQKILVYDLGGGTFDVSILEIGDGVFEVLATSGNNRLGGDDFDQKIIDYLVD
                     MFKKDQGIDLSKDRMAMQRIKEAAEKAKIELSGMTTTEINLPYITADATGPKHMNYTL
                     SRAKFDELTSDLVEKTMVPTRQAMKDAGLTPDDIGKVLLVGGSTRIPAVQEAIRKYLG
                     KEPFKGINPDECVAIGAAIQAGVLTGDVKDLLLLDVTPLSLGIETLGGVFTKLIERNT
                     TIPTRKSQIFSTAADNQTSVEIHVLQGERELAKYNKSLGRFQLTGIMPAPRGVPQIEV
                     TFDIDANGIVNVSAKDMGTGAEQKITITASSNMTDEDIERAVKEAEEFAAQDKEIKER
                     IDAKNNADNLVYQSEKILKDMGDKLDPGDKDNIEKEIENVRNAMAADNTEEIKEATEK
                     LQQAFYAVSEKVYRQTANPQEGAADADNAQSDENVYDADYKVVDEDEEDNK"
     gene            complement(3333..>3470)
                     /gene="grpE"
                     /locus_tag="PLI11_06785"
     CDS             complement(3333..>3470)
                     /gene="grpE"
                     /locus_tag="PLI11_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPZ52606.1"
                     /translation="NPDLHNAVMHVEDENASANEIVEVFQKGYKLNERVIRYSMVKVA
                     N"
BASE COUNT          844 a          871 c          623 g         1132 t
ORIGIN      
        1 tcttgtttgc atcccatttt gcaataagtt tcatattaca cctccgtaaa aaaataaaaa
       61 gagaaagaca tacaactgca aaacagatta tattctttct ctttcaatca tgcctgtgat
      121 cacaccccat ttatttctca cagggtgatt atactacatc catattttat ttcaaccctt
      181 atttctactt tttacgacca aaaaaagacc ttctcccatt ggaactgttt ccagtaatgg
      241 aagcctgcag ctgtcttaga agatcttttt gagtttcatt caacttcttt ggaatggcaa
      301 ctgaaaccct tacaatctga tccccacggc cagaagaatg aagtttgggt attccctttc
      361 ctttaagctt gaaagaaact cctggttgtg tgccttcagg aattgtaagt tcaacctttc
      421 catccagggt gtcaaccaca ataacatctc ccaaagcagc ctgtatcata tcaatggtaa
      481 catccagata aacgtcaaac ccttcccttc ttagaagttt gtgtctctgt actcttattg
      541 atatgattaa atctcctgga gggcctcctt ttgaacccgg ttcaccctgg ccggacatgg
      601 atatagcctg gccatggtct attcccgcag gtaccttcac cttaatagta acattctttc
      661 ttaccttgcc gttaccggaa caaacagtac aagggtccgt aacagttttg cctgtaccgc
      721 cgcacttgtc gcaagtccga acatttacaa acgaaccaaa aggtgtcgcc tgtttgactc
      781 ttacctgtcc tgaacctccg cagttagtac aactgctggt agatttggat tttgaacctg
      841 ttccttcaca agtactgcag cttacgtact tgttgacaga gacatccttt tccgtaccaa
      901 aagccgcttc ctcaaaagtt atggtcatgg tcgttcttat gtcagcacct tttacgggtc
      961 cccttgactg cctggagcca aaccctgtgc cgaaaaagtc aaaaatgtca tcaagtccac
     1021 caaaaccaaa tcctccgaag tcaaaacctc caaaaccgcc gcctgcagcg ctgttggggt
     1081 cgaaagcagc atgaccaaac ctgtcatact gggctctctt ttcagagtcg gacaggactt
     1141 cgtaagcttc ggttgcttcc ttgaactttg cttccgcttc tttgttatca ggatttacgt
     1201 ccggatggta tttctttgca agttttctat atgctttttt tatttcatca gtgctggcat
     1261 ccctactcac gccaagcact tcgtagtaat ctctctttgt gctcatccat atctcccttt
     1321 ttgagtgtcc ttgtcaaaca caacaatatc tttgccacca aatcctggcg gcaaagatat
     1381 attatacatt acacttgctt taaaaacaac ttcatcagtt acttgttatc ttcctcgtct
     1441 tcatcaacta ctttgtagtc agcatcgtaa acattctcat cagactgagc attgtcagca
     1501 tcagcagctc cttcttgagg atttgctgtt tgtctgtata ccttttcaga tactgcgtag
     1561 aacgcctgct gcagcttttc agttgcttcc tttatctctt cagtattgtc agcggccatt
     1621 gcatttctga cattctcaat ttccttttca atattgtcct tgtcacctgg atcaagtttg
     1681 tcacccatgt ctttcagtat cttttcagac tgataaacta ggttgtcggc attgttcttt
     1741 gcatctattc tttcttttat ttccttgtcc tgagcagcaa actcttccgc ttccttgact
     1801 gctctttcaa tatcctcgtc agtcatattg gaagaagcgg taatggtaat cttctgctcc
     1861 gctccggtac ccatgtcctt tgcagacaca ttaacaatac cgttagcgtc aatgtcaaat
     1921 gtaacctcaa tctgtggcac ccctcttggt gcaggcatta taccagtaag ctggaatctt
     1981 ccaagagact tgttgtactt ggcaagttct ctttcaccct gcagaacatg aatttcaaca
     2041 ctggtctggt tgtctgcagc agttgagaat atctggctct tccttgtagg aattgttgta
     2101 ttcctttcaa taagttttgt gaaaacaccg cccagagtct ctataccaag tgaaagtggt
     2161 gtaacgtcaa ggagaagcag atctttgaca tcgcctgtaa gaacgcctgc ctgaatcgca
     2221 gctccaatcg caacgcactc gtcagggttg attcccttga aaggttcttt gccaagatac
     2281 tttctgattg cttcctgaac tgcaggaata cgtgttgatc cgccaaccaa aagcaccttg
     2341 ccaatatcgt caggagtcag tcctgcgtcc ttcattgcct gccttgtcgg taccatggtc
     2401 ttctcaacca gatcggatgt aagttcgtca aatttcgctc tgctcaatgt gtagttcata
     2461 tgctttggac cggtagcatc tgcagtaatg tatggcaggt ttatttcagt tgtagtcatg
     2521 cctgaaagtt caatcttggc tttttcagca gcttccttta ttctctgcat tgccatcctg
     2581 tcctttgaaa gatcaattcc ctgatccttc ttgaacatat ctaccaggta gtcaataatt
     2641 ttctggtcga agtcgtctcc accaagacgg ttattaccgc tggtagcaag aacttcaaat
     2701 actccatcgc ctatctcaag aatggatacg tcaaaagttc ctccacctag gtcgtataca
     2761 agaatcttct gctcgttttc cttgtcaaga ccgtatgcaa gagctgcggc agtaggctcg
     2821 tttatgattc tcaatacttc aagtcctgca atacgtcctg catcttttgt tgcctgtctt
     2881 tgtgagtcgc taaagtatgc aggaacagtt ataacggcct tacttacctt ttcaccaaga
     2941 tacgcttccg catcagcctt gagcttctga agaatcattg cggaaatctc gggtggagta
     3001 tatctttttt cgtctatggt gattctcctg tcagtaccca tgtcgcgctt tatggacata
     3061 atggttctgt cagggtttgt tattgcctgc ctctttgcgg cagctccaac cagcctttct
     3121 gcaggttttc cctctatagc tttggtaaac gccactacgg acggtgtagt tctggatcct
     3181 tctggatttg gtattacgac gggttcgccg ccttcaataa ctgctacgca tgagttcgta
     3241 gttcctaagt caatgccaat aactttcgac ataatatctc ctcctaataa cttattttat
     3301 tcaaattata taatcaaatg cattaaacaa tgctaatttg ccacttttac catactgtag
     3361 cgtataactc tttcattgag tttgtatccc ttttgaaaaa cctcaacaat ctcatttgca
     3421 ctggcgttct catcctccac atgcattaca gcattatgca agtctggatt
//
LOCUS       DAONVY010000231         3469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18815_length_3469_cov_4.302871, whole genome shotgun sequence.
ACCESSION   DAONVY010000231 DAONVY010000000
VERSION     DAONVY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18815_length_3469_cov_4.302871"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1093)
                     /locus_tag="PLI11_06790"
     CDS             complement(158..1093)
                     /locus_tag="PLI11_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52607.1"
                     /translation="MIEVKSNYEVLGLPENATLEDVERKYGALIRQYKKRVDEKGQTY
                     EDLEYYRAITKAYNELTGKNQNLEDDNPTSIIPYSVRKRFEIIQAHLSQYYFAIFAVI
                     LLLAMGTLFILQYRDNRKTDFGIKFVGAFSVANPTDFNEEIVKRSKVTKYPVISFFTV
                     TTETQLTPAVQSQATSFLSQLMVGKNLDVILLDKESYDAYVRQGAFLALDDILKEYSD
                     REWYDSLRKFDYKLNPDIEEGMQPVEAVYGVDVTNTTFFDGTSLEWLHDVDKGQEKVM
                     ILTIARTSMNKEKAIDFMIEILDTMKLEQNEENSQ"
     gene            complement(1099..2601)
                     /gene="lysS"
                     /locus_tag="PLI11_06795"
     CDS             complement(1099..2601)
                     /gene="lysS"
                     /locus_tag="PLI11_06795"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPZ52608.1"
                     /translation="MEENLNNEENIFWEEDINEQMQVRRNKLSDLKNSGKDPYVITKF
                     DVTHHSQPIIENYGELEDQEVSIAGRIMLKRIMGKASFAHILDSKGLIQIYVRKDEVG
                     DESYSDFKVYDIGDIIGIRGIVFKTRTGEISVKAKEVTLLSKALNPLPEKKHGLRDID
                     LRYRQRYLDLIVNREIKETFVKRSLIIKEIRKFLDNKGYIEVETPVLHNIAGGAAARP
                     FVTHHNTLDIDLYLRIALELHLKRLIVGGFDKVYEIGRVFRNEGMSPRHNPEFTLLEL
                     YEAYTDYNGMMDLTEEMFRVVSRNVLGTTKIVYGDVEIDLEKPFERISMVDAVKKYKG
                     IDFSNITSVEEARKLADEHKVEYEPHHEVGDILNLFFEEFCEEHLIQPTFLMNHPVEI
                     SPLAKRMPEDPRYTERFELFIVGREHANAFSELNDPIDQRERFEIQAQLKAAGDEEAN
                     DVDFDFLNALEIGMPPTGGLGIGIDRMVMLLTNSQSIRDVLLFPTMKPRD"
     gene            complement(2601..3101)
                     /gene="greA"
                     /locus_tag="PLI11_06800"
     CDS             complement(2601..3101)
                     /gene="greA"
                     /locus_tag="PLI11_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPZ52609.1"
                     /translation="MVEKVTHLTKEGLRYYQNLLAEIKGKRALEIAKRIEDARSFGDL
                     SENSEYDEAKAAQAMNEAERIRIESILKNYVLIDDTNLTTDEVRLGLVITLYDYEFEE
                     EVDYYLVGSSEANPLEGKISNESPVGQAILGKKVGDEVEVKTPGGEIVKYKIVDIKIP
                     EKKDVV"
     gene            complement(3143..>3469)
                     /locus_tag="PLI11_06805"
     CDS             complement(3143..>3469)
                     /locus_tag="PLI11_06805"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPZ52610.1"
                     /translation="KIRKGMTYVKEYWGEGSNRARNWQRYDLGDTPGMTFEEGVDAYV
                     PYAGPLRDNVEVTINKLKATMCNCGVLSIPELHKNARLTLVSATSIVEGGAHSVILKE
                     KDYRNK"
BASE COUNT          912 a          841 c          569 g         1147 t
ORIGIN      
        1 gtttaactgg cttgtttcta aattaactca taagaattaa acgagttaca tgatacgctg
       61 tttagttttc aatgtgctgc tgtcgttgga cagctcgatt agtatatcac ctaaaaaagt
      121 gtttgtcaac catgatttta aaaattttcg atatttttca ctgggaattt tcctcatttt
      181 gttcaagctt cattgtatcg agaatttcta tcatgaaatc aattgccttc tccttattca
      241 tactggttct ggcaatagtc agtatcataa ccttctcctg tcccttgtcc acatcatgca
      301 gccactctag ggacgtgcca tcaaaaaacg tcgtatttgt tacatcaaca ccgtaaacag
      361 cttctacagg ctgcattccc tcttcgatgt ccggattgag cttgtaatca aactttctca
      421 aagagtcata ccattccctg tcggaatact cttttagaat atcatccaga gcaagaaaag
      481 ctccttgcct aacatacgca tcatagcttt ccttatctaa taaaatgaca tcaagatttt
      541 taccaaccat aagctgcgac agaaacgatg tagcctggct ttgaaccgca ggtgttaact
      601 gggtctcagt ggttaccgtg aaaaaggata ttacagggta ttttgtaact tttgatcttt
      661 tcactatctc ctcattgaaa tccgtaggat tcgcaacact gaacgcacct acaaacttga
      721 ttccaaaatc ggtctttcga ttgtccctgt actgaagaat aaacaaagta cccatggcca
      781 gcagtaagat taccgcaaaa atcgcaaaat agtactggct gagatgtgcc tgtattattt
      841 caaatctttt tcgcacgctg tatggaatga tggatgttgg gttatcatcc tcaagatttt
      901 gattcttccc tgtaagctcg ttataggctt tggtgatggc tctgtaatat tccagatcct
      961 cgtaagtttg tcctttttcg tctactctct ttttatactg ccgtataaga gcaccgtatt
     1021 ttctttcaac atcttccagg gttgcatttt caggaagtcc caacacctca tagttgcttt
     1081 tcacttcaat cacccatgtc aatctcttgg tttcatcgtc gggaacaaca gtacatctct
     1141 aatggattga ctgtttgtga gcagcataac cattctgtct ataccaatac ccagaccacc
     1201 agttggaggc attcctattt cgagtgcatt caggaagtca aaatccacat cgtttgcttc
     1261 ttcatcacca gcagccttga gttgcgcctg tatttcaaaa cgttctctct gatctatagg
     1321 atcattaagc tcggaaaacg catttgcatg ctcacgtccc actataaaca actcgaacct
     1381 ctcggtatat ctcgggtcct cgggcattct tttggcaaga ggagatattt caacgggatg
     1441 attcatcagg aaggtgggct ggataaggtg ttcttcacag aactcctcaa agaaaagatt
     1501 taatatgtct cccacttcat gatgaggttc atattcaact ttatgttcat cggcaagctt
     1561 ccttgcctct tctacagatg taatgttgct gaaatctata cccttgtact tcttaacagc
     1621 atccaccatg gatatgcgtt cgaaaggctt ttccaagtca atctccacat ctccatagac
     1681 aatctttgtg gttcccaaca cattcctgct tacaaccctg aacatctctt cagtaaggtc
     1741 catcattccg ttatagtcgg tgtaagcctc gtaaagctcc agcaaggtaa attcaggatt
     1801 gtgtcttggg gacatgccct catttctgaa aacccttcct atttcatata ccttgtcaaa
     1861 cccgccaaca atgagcctct tcaagtgcag ctcaagagct attctcagat acaagtcaat
     1921 gtcaagagtg ttgtgatgag ttacgaaagg tctcgcagca gcgcctcctg caatattatg
     1981 aagaacagga gtctccactt caatgtagcc cttattatca aggaacttcc ttatctcctt
     2041 gataataagg gatcttttta cgaaagtttc cttaatctct ctgtttacta tcaggtcaag
     2101 atacctctgt ctgtatctaa gatcaatatc cctaagtccg tgttttttct ctggcagcgg
     2161 attcaaagcc ttgcttaaaa gggttacttc ctttgcttta accgagattt cacctgtcct
     2221 ggttttgaaa actatacccc ttataccaat gatgtcacca atatcataga ccttgaagtc
     2281 agagtaactt tcatctccta cttcatcttt tctgacgtaa atttgaatca gacccttgga
     2341 atcaagaatg tgggcaaaac ttgccttgcc cataatgcgc ttcaacataa tacgacccgc
     2401 tatactcact tcctgatcct caagctcacc gtagttctct attattggct gagaatgatg
     2461 agttacatca aacttggtaa ttacgtatgg atccttacca ctgttcttca ggtccgacaa
     2521 cttgttgcgt ctgacctgca tctgttcatt tatatcttct tcccaaaaaa tattttcttc
     2581 attattcaaa ttctcttcca ttaaacgaca tccttctttt ctggtatctt tatatcaaca
     2641 atcttgtact ttacaatttc accacccggt gtttttacct caacctcgtc tcctaccttt
     2701 ttgcccaaaa tcgcctggcc aacaggagac tcgttagaaa ttttaccttc taaaggattg
     2761 gcttcgcttg agccaaccag gtagtaatca acttcctctt caaactcgta atcatacaaa
     2821 gtgattacga ggcccaatct tacttcatcc gtagtaagat tcgtatcatc tattaaaaca
     2881 taattcttta gaattgattc aatccttatc ctttcagctt cattcattgc ctgggcagcc
     2941 tttgcctcat catactccga gttttcagac aaatccccga aactcctggc atcttcaatc
     3001 ctttttgcga tttccagtgc tcttttgcct tttatctccg caagaagatt ttgataatag
     3061 cgcaatcctt ctttcgtgag atgagttact ttttccacca tactgtcact ccttcattaa
     3121 tccattctca atatattcaa cgctatttat tgcggtaatc cttttccttc aagatgacac
     3181 tgtgtgctcc gccttcaaca atgcttgtag cggataccaa agtaagtctt gcatttttat
     3241 gaagttcagg tatagacaat acaccgcaat tgcacatggt agcctttaat ttatttattg
     3301 taacttcaac gttgtctctg agcggacctg catatggaac gtatgcatca acaccttctt
     3361 caaacgtcat gccaggggta tctcccaaat catatctctg ccagttcctt gccctgttgg
     3421 aaccttctcc ccagtattcc ttcacatagg tcatgccttt tcggatctt
//
LOCUS       DAONVY010000232         3468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_18828_length_3468_cov_3.735423, whole genome shotgun sequence.
ACCESSION   DAONVY010000232 DAONVY010000000
VERSION     DAONVY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18828_length_3468_cov_3.735423"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..2703
                     /locus_tag="PLI11_06810"
     CDS             226..2703
                     /locus_tag="PLI11_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPZ52611.1"
                     /translation="MQNERNRFINVLKTNIPAVIVILIMTIALKIALDYNVILSFIIL
                     CIAYVVLGIIVEFILEKIEGTRTVQEPQYRSERQVKPKLVDLSDDSDITIKQRPVRKI
                     TTSPNAAEVAQRLRKTKLNFDDLLTLSDTPQTVRREEKVLASKVSVINDYSTLLNKAK
                     QTQEEPEAAQTEIKSTVSVEKITDENYNKIESKKTDIDESYTLDDNDFTVRPAEDLES
                     VSKRIKHEVEKRLSDSKKTIFPRRKKGTSLMEDLTFDNQEEEKKTENIDTVEKLDDFK
                     IVREDDKKDDEALEDIFFEADGVEGNDMAVADTDLINELYTEKEEEPKKKTTFLEKSF
                     PLKEKSSRQSYEKPSQELIKPSIQRDVNTNERDEELRKRGEKLITTLQSFGVGAKIVG
                     ISKGPTVTRFEIQPNAGVKVSKIVSLADDIALNLAATGVRIEAPIPGKAAIGIEVPNK
                     EVDIVYLHDVLSSSKYIKAPSKLSIALGMDISGNIIVGDIARMPHLLIAGSTGSGKSV
                     CINSIILSILFKASPDEVKFIMIDPKVVELGVYNGIPHLLIPVVTNPKKAASAVAWAV
                     NEVENRYAMFAQHNVRDLASYNKLASKKNLESGKLPQIVIIIDELADLMMVAANEIED
                     SIIRLAQKARAAGVHLVVATQRPSVNVITGLIKANIPSRIAFAVASQVDARTILDHAG
                     AEKLLGRGDMLYHPIGASKPLRVQGAFVSDSDVEKVVEFVKSSYGPVEYDEEVASMIE
                     STQNAETIVQSGNGENPDDELIEQACELAFEFNQLSASFLQRKLKIGYSRAARIIDTL
                     EDRGLLSAADGSKPRQVLMSRREWYGS"
     gene            complement(2698..3450)
                     /locus_tag="PLI11_06815"
     CDS             complement(2698..3450)
                     /locus_tag="PLI11_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPZ52612.1"
                     /translation="MKLVSWNVNGLRACMKKGFSDFFNKVDADIFCIQETKMHQSQGA
                     PEFEGYEQYWNDAERKGYSGTAIYTRTSPLSVSYGLGIEEHDNEGRVITIELEEFFLI
                     CVYTPNSKTELERLDYRMVWEDEFRNYALKLEQMKPVIICGDLNVAHKEIDLKNPNTN
                     RRSAGFTDEEREKFTQLLDAGYVDTYRHLYPDVTGAYTWWSYMFNARKNNAGWRIDYF
                     LVSEKLKNRIEDAIIYSDVYGSDHCPVGLILS"
BASE COUNT         1122 a          640 c          749 g          957 t
ORIGIN      
        1 actcataatg agctgattga gaagaaggga atatactaca atctggtgct tgcccagctc
       61 agaatgacaa ggctggttgg tgacgaggag atagtggaag aggaaatcat tcaggaaata
      121 gtgtaagaat ttgaaaattt atatatgtgt caaaagtatt gctcatcctt taaggatgtg
      181 ctatactttt aaagaagccc tattattatc atggagtgta aaaaaatgca gaacgaaaga
      241 aatagattca taaatgtatt aaaaactaat ataccagcag ttatagtaat tttaataatg
      301 acaatagctt taaaaattgc actggattac aatgtaattc tgtcttttat catactttgc
      361 attgcatatg ttgtactagg gataattgta gaatttattc ttgagaaaat agaaggaaca
      421 agaacagtac aggaacctca gtatagaagc gagagacaag taaagcctaa acttgtcgat
      481 ctttctgatg acagcgatat tacaataaag caaagaccgg tcaggaaaat tacaacatct
      541 ccaaatgctg ctgaggtggc tcagcgtcta aggaagacaa agcttaattt tgatgatttg
      601 ttgacccttt cagacacccc acagacggtt agaagggaag aaaaggtgtt agccagcaag
      661 gtcagcgtta taaatgacta ctcaacgctg cttaacaaag ccaaacaaac acaagaagag
      721 ccagaagcgg cgcaaactga gattaaaagc acggttagtg tggaaaaaat aaccgacgag
      781 aattataaca agattgaaag caagaaaact gatattgacg aatcatacac tcttgatgac
      841 aatgatttta ctgtaagacc tgctgaagat cttgagtccg tttcaaagag aataaaacat
      901 gaggttgaaa aacgactttc cgattctaaa aagaccattt tccctagaag gaaaaaaggt
      961 acttctttaa tggaagattt gacatttgat aatcaagagg aagaaaagaa aacagaaaac
     1021 atagatactg ttgaaaagct tgatgatttt aaaatagtga gagaagatga caaaaaggat
     1081 gacgaagctc ttgaagatat tttctttgag gcggatggcg ttgaaggcaa cgacatggct
     1141 gttgctgata cggatttaat aaacgagctg tacaccgaaa aggaagaaga gcctaaaaaa
     1201 aaaacaacct ttttggaaaa atcttttccg ttaaaagaaa aaagtagccg acaaagttat
     1261 gaaaagcctt cccaggagtt gattaaaccc agtattcaaa gagatgtcaa tactaatgaa
     1321 agggatgagg agctcaggaa aaggggagag aaacttatta ctactttaca aagttttggt
     1381 gtaggcgcga agattgttgg aatatccaaa gggcctactg ttacccgttt tgaaattcaa
     1441 ccgaatgctg gtgtcaaggt aagcaaaatc gtttctcttg cggatgacat tgccctaaat
     1501 ctcgcagcta ccggtgtaag aatagaagcg cccataccag gaaaagctgc tattggaata
     1561 gaagtaccca acaaggaagt ggacatagta tatctgcatg atgttttgtc cagcagcaag
     1621 tacataaaag ctccatccaa gctatctatt gcacttggaa tggatatctc tggcaatatt
     1681 attgtgggtg acatagcaag aatgcctcac ttgctgattg ccggttccac tggctcggga
     1741 aagagtgtgt gtattaactc tataatcctc agtatactgt ttaaggcaag tcctgatgaa
     1801 gttaaattta taatgatcga tccgaaagtc gttgaacttg gcgtgtacaa cggcatacct
     1861 catctattga tacctgttgt cacaaatccc aaaaaggcgg caagtgccgt tgcgtgggct
     1921 gtgaacgagg ttgaaaatag atatgccatg tttgctcagc ataatgtaag ggatttggct
     1981 tcgtacaaca agcttgcttc caagaagaat ctggagagcg gaaagcttcc tcagattgta
     2041 ataattattg acgagctggc ggatttaatg atggtagcgg caaatgaaat agaggactcg
     2101 attataaggt tggcgcagaa agcacgtgct gcaggagtgc atttagttgt tgcaacccaa
     2161 agaccttcag taaacgtcat caccggcctc ataaaagcca acataccttc acgtatcgcg
     2221 tttgcagttg catcacaagt ggatgcaagg acaatacttg accatgctgg tgcagagaag
     2281 ctgttgggaa gaggagatat gctgtatcat cctattggag cttcaaagcc tttaagagtt
     2341 caaggtgcct ttgtatcaga tagtgatgtt gaaaaggttg ttgagtttgt taaatccagc
     2401 tatggacccg ttgaatatga cgaagaggtt gccagcatga ttgaaagtac tcaaaacgct
     2461 gaaacaatag tacagtctgg caatggggaa aatcccgatg atgagctgat tgaacaggct
     2521 tgcgaactcg catttgagtt caaccaattg tcagcttcat ttttgcagag gaagttgaag
     2581 attgggtact cgagggcggc aagaattatt gacaccctcg aggatcgtgg tctgctgtca
     2641 gctgccgatg gcagcaagcc cagacaggtt cttatgagca gaagagaatg gtatggctca
     2701 tgacagaatt agccccaccg gacagtggtc actgccatac acatcggaat atattattgc
     2761 atcttcgatg cggttcttta atttttccga gaccagaaaa tagtctattc tccagcccgc
     2821 attgtttttg cgggcattaa acatataact ccaccaagtg tatgctcctg taacatcagg
     2881 ataaagatgt ctgtatgtat cgacataccc tgcgtccaac agctgagtaa atttttctct
     2941 ttcttcgtct gtaaatccgg cactgcgtct gttggtgttc ggatttttca gatctatttc
     3001 tttatgagct acattaagat cgccgcaaat tatgactggt ttcatttgct caagtttcag
     3061 tgcataattt ctgaactcat cctcccatac cattctgtaa tcgagccttt caagttcagt
     3121 ctttgaatta ggtgtatata cgcaaattag aaagaattcc tccaattcaa tggtaataac
     3181 ccttccttca ttatcgtgct cttcgatgcc aagtccatag cttacggaca atggactcgt
     3241 tctagtatat attgctgtac cggaataacc ctttctttca gcatcattcc aatactgctc
     3301 gtaaccttca aattccggtg caccttggct ttggtgcatc ttggtttcct gtatgcagaa
     3361 tatatctgca tcaactttat tgaaaaaatc agaaaacccc tttttcatac acgctcttaa
     3421 tccgtttaca ttccatgata caagtttcat tttctacctc cataattc
//
LOCUS       DAONVY010000233         3434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19060_length_3434_cov_3.441551, whole genome shotgun sequence.
ACCESSION   DAONVY010000233 DAONVY010000000
VERSION     DAONVY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19060_length_3434_cov_3.441551"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLI11_06820"
     CDS             <1..200
                     /locus_tag="PLI11_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52613.1"
                     /translation="NTVKYYIKNAALLGIKVYLDDSQQEATMDDIDKVSDTALYRMTK
                     TYKEDGSLKEILFVIESQATA"
     gene            226..636
                     /locus_tag="PLI11_06825"
     CDS             226..636
                     /locus_tag="PLI11_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52614.1"
                     /translation="MAGKVIANFLSIFVFVYLILSGVSFCIYAGVNERTNDLLFDVAE
                     TVSTKGMLSDEVWAYLNEDLSKMGDFCVEMKLEVCVMAGQTDTYYKIEDILNRKLDPG
                     DRLTIVAYSLEPSIFEKVTGVVLRPAGVKVSIIA"
     gene            645..1136
                     /locus_tag="PLI11_06830"
     CDS             645..1136
                     /locus_tag="PLI11_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52615.1"
                     /translation="MHQLVAWVVAIWMFLMIALTGINTINEGFLKRDEKRNLNLLGRE
                     IVNCISFDDGNLVLDRDKCFESVKALPTSQNVKVMIFVYEDVVAGMTESESETVYVED
                     DERSVCVNRVLNMLIDKAARIEIAYNGVEDSFFNELGDGIYVFLVSEQESFLSVSGFS
                     INI"
     gene            1208..>1542
                     /locus_tag="PLI11_06835"
     CDS             1208..>1542
                     /locus_tag="PLI11_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52616.1"
                     /translation="MKVFYFTKIDSAEEVMDCGIKLNQYFEREITVNEFKKRFLLTYL
                     HPADSTKFYDDTYKAVKIKAPDDTSYIAEGALYDEENMSLYESSLVPIREYRLGTYRK
                     PECLIQCTLL"
     assembly_gap    1543..1642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1643..1971
                     /locus_tag="PLI11_06840"
     CDS             <1643..1971
                     /locus_tag="PLI11_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52617.1"
                     /translation="YRLGTYRKPECLIQCTLLPHQIEDFDRRRDEPILYDCSENLYLN
                     RVLYKVREEFSFFDDLALERYYDVLVEEGKYIKEECNRFNVYINNDDNEIITIRKWRQ
                     LDVGEY"
     gene            1955..2245
                     /locus_tag="PLI11_06845"
     CDS             1955..2245
                     /locus_tag="PLI11_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52618.1"
                     /translation="MSENINRGKNNRRAPNHRRRMDKDTEQGINENRKNVSGARRNEN
                     YAKSGKQTRTHTKDFKKIKVEETIEDIAKDIARIEKEIDLEIKEIMALKLGL"
     gene            2262..3422
                     /locus_tag="PLI11_06850"
     CDS             2262..3422
                     /locus_tag="PLI11_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPZ52619.1"
                     /translation="MDALKKHEEWQGKIEVKVRAQVSNRDELAVAYTPGVAEPCKEIS
                     KDVDKSYIYTRRWNTVCVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKEFGDVDAIPL
                     CIRSKDVDEIVKTISLLSGSFGGVNLEDISAPRCFEIERRLKEVCDIPIFHDDQHGTA
                     VVVLAAMLNSLKIVNKKLEDVEVVVNGSGAAGIAITKLLMSMGLKNVVLCDRKGAIYK
                     GREDLNSEKVMMADITNTQMKKGTLAEVIKGADVFIGVSGPNMVTPEMVQSMAEEPVI
                     FALANPIPEILPDDAKNAGARVVGTGRSDFPNQINNVLAFPGIFRGALDVRASDINDE
                     MKIAAAKALADLIHENELNPDYVIPDPFNKDVARVVAEAVAQAARDTGVARI"
BASE COUNT         1043 a          526 c          860 g          905 t
ORIGIN      
        1 agaatactgt aaaatattat atcaagaatg ccgcattgct tggcataaag gtgtatcttg
       61 atgatagcca gcaggaagcg acaatggatg acattgataa ggtcagtgat accgctctgt
      121 acaggatgac caaaacctac aaggaagacg gttcgttgaa ggaaatactg tttgtgattg
      181 aatcacaggc aactgcgtag gcgatttttt tagggaggtg tctggatggc cggcaaggtt
      241 attgctaact ttctttcgat attcgtattt gtgtatttga tattgtcggg cgtatcattt
      301 tgcatatatg cgggtgtcaa tgaaagaacc aacgatcttt tgtttgatgt ggctgaaact
      361 gtcagcacaa aaggaatgtt gtcggacgag gtttgggcgt atctgaacga agacctgtca
      421 aaaatgggag acttttgtgt tgaaatgaag ctggaggtct gtgttatggc cggtcagaca
      481 gatacatatt acaaaattga agacatattg aatagaaagc ttgatccagg cgacaggctt
      541 acgattgtgg cgtacagcct ggagccttcc attttcgaaa aggttaccgg agtggtctta
      601 agacctgcag gtgttaaggt ttcgataatt gcgtaggtgg taatatgcat cagctggttg
      661 catgggttgt tgcaatctgg atgtttttga tgattgcatt aacgggcatt aatactatta
      721 atgaaggatt tttaaagcgg gacgaaaaaa ggaatttgaa tctattaggc agagagattg
      781 ttaactgtat ttcctttgat gacggaaatc tggtgcttga cagagataaa tgctttgaaa
      841 gtgtgaaggc tcttcctaca tctcaaaatg tcaaggttat gatatttgtt tacgaggacg
      901 ttgttgcagg catgacggaa agcgaatcag aaactgttta tgttgaagat gatgaaaggt
      961 cagtttgtgt aaaccgggtc ttgaatatgc tgatcgataa ggcagcgagg atagaaattg
     1021 catataatgg ggtggaggat tcttttttta atgagctggg tgatggcatt tacgtatttc
     1081 tggtatctga acaagaaagt tttttaagtg tatcgggttt cagcattaat atttgacgtt
     1141 ttatttgcaa taactccttg tttttgctat aatctctaat tgagatcggc atgggaggag
     1201 tcatacattg aaggtgtttt attttacaaa aattgacagc gctgaggaag taatggattg
     1261 cggcataaaa ctgaaccagt atttcgagag ggagataacg gtaaatgaat tcaaaaaaag
     1321 atttcttctc acgtatctgc atcctgccga cagcacaaag ttttatgatg atacctacaa
     1381 ggccgtgaaa ataaaagcgc ctgatgatac ttcctacata gcggaaggag cgctgtatga
     1441 tgaggagaat atgtctcttt atgaatcaag tcttgttccc atccgtgagt acaggctcgg
     1501 tacttacaga aaacccgaat gcctgataca atgcaccttg ctnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnagtacagg ctcggtactt acagaaaacc cgaatgcctg
     1681 atacaatgca ccttgctacc acatcagatt gaggattttg acaggaggcg ggacgaaccc
     1741 attttgtacg attgttctga aaatttgtat ttgaacaggg tgttgtataa agtaagggaa
     1801 gagttttcgt tttttgatga tttggcactt gaaagatatt atgacgtgct ggtggaagag
     1861 ggaaaataca ttaaagaaga gtgtaataga tttaatgtat atatcaacaa tgatgacaat
     1921 gagattatta ccataaggaa atggaggcag ttggatgtcg gagaatatta acaggggtaa
     1981 gaacaatcga agagctccca atcatagaag aagaatggat aaggataccg agcagggcat
     2041 aaacgaaaat aggaagaatg tctctggagc aaggagaaat gaaaactacg caaagtccgg
     2101 caagcaaacc cggacccaca cgaaagattt caaaaaaatc aaggttgagg aaaccataga
     2161 agatattgca aaagatatag caagaataga aaaggaaata gatttggaaa ttaaagaaat
     2221 aatggcttta aagctaggac tataatttat ggagggtatt tatggacgca ctaaaaaagc
     2281 atgaagaatg gcaaggtaaa atagaggtaa aggtaagggc gcaggtaagt aacagagacg
     2341 agcttgctgt ggcctataca ccaggagttg cagagccctg caaggaaata agcaaggatg
     2401 ttgacaaatc ctatatttac acaagaagat ggaacactgt atgtgttgta acggacggta
     2461 ctgccgtact cggccttggc gacattggtc ctgaagcagg aatgccagtt atggaaggaa
     2521 aatgcgttct tttcaaggaa tttggagatg tggatgcaat tcctctttgt attcgctcaa
     2581 aagatgtgga tgagatagtc aagactatca gtcttttgtc cggcagtttt ggcggcgtga
     2641 atctggaaga catttcagca ccaagatgtt ttgaaataga gagaaggttg aaggaagtat
     2701 gcgatatccc catattccat gacgaccagc atggaacggc tgttgttgtt cttgctgcca
     2761 tgttgaatag ccttaaaatc gtcaacaaga agctggagga tgtcgaagtg gttgtgaatg
     2821 gcagtggagc tgccggtata gccattacaa agcttctcat gtccatgggg cttaaaaatg
     2881 ttgtcctgtg tgacagaaag ggcgccatct acaaaggcag ggaagatttg aacagtgaaa
     2941 aggtcatgat ggctgacatt accaacactc aaatgaaaaa aggaactctt gcagaagtaa
     3001 tcaagggcgc agatgtattt ataggtgtgt cgggtccgaa catggttact cctgagatgg
     3061 ttcagtccat ggcagaggaa cctgtgatat ttgctcttgc aaatcccatt cctgagattt
     3121 tgcctgatga tgcaaagaat gcaggagccc gtgtggttgg caccggcaga agcgattttc
     3181 ctaatcagat taacaatgtc cttgcattcc cgggcatttt cagaggtgcg cttgatgtaa
     3241 gggccagcga tataaatgat gaaatgaaaa ttgcagcagc aaaggctttg gcggatctga
     3301 ttcacgaaaa cgagcttaat cccgattatg taatacccga tcctttcaac aaggatgtgg
     3361 caagggttgt tgcggaagct gtagcacagg ctgcaagaga tacaggtgta gcaagaatat
     3421 gatgccaata gttg
//
LOCUS       DAONVY010000234         3427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19108_length_3427_cov_3.911032, whole genome shotgun sequence.
ACCESSION   DAONVY010000234 DAONVY010000000
VERSION     DAONVY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19108_length_3427_cov_3.911032"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2695
                     /locus_tag="PLI11_06855"
     CDS             <1..2695
                     /locus_tag="PLI11_06855"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPZ52620.1"
                     /translation="LSGRSYVYQAMRITGCSDPTVPVEETREGKLPQRVITTTAAQGY
                     SSYGNQIGVPAGFLKEIYHPGYIAKRMELGAVIAAAPQENVVRSKPEKGDLIILLGGR
                     TGRDGIGGATGSSKEQTVDAIQTLGSEVQKGNPPLERNIMRLFRKKEVSTLIKKCNDF
                     GAGGVSVAIGELAPSISINLDLVPLKYDGLDGTEIAISESQERMAVVVSEYDAEKFIQ
                     YANEENLEAVVVAEITDTGRLKMYWRGDLIVDIRRDFLDTNGVTQKACANVEPFDLYI
                     EKRNAESIKDEWVKNLMNLNSCSQKGLVEMFDSTVGGNAVIMPLGGKYQLTPADGMVS
                     KIPVLNGETKTGSIMTFGYDPYLSEKSPFHGSMFAVIDSLAKVTSLGGDFSKARLTFQ
                     EYFERLYDVPTKWGKPLAALLGAFHVQKELKIPSIGGKDSMSGTFMEIDVPPTLVSFA
                     VSVLEDVGKVVTQEFKKANSRVILYKLPVNEEFMPDFESLKKNYMKIHTLADQGRIKA
                     AMAVRFGGIAETISLMCFGNMIGFDLKNFDNLFDKMYGSIIIELDDSASDKDLEGLDY
                     IDLGTTIEYKVVRLGDEIVSLDELLENWRKPLDDIFPEKASFVNNDVNIPEYDEKDRT
                     YSSIKVAKPKVFIPLFPGTNGEYETIKAFEDAGAVVETFVVRNRSGKEIEESIKVIKS
                     MIENSQIVALPGGSSAGDEPEGAGKFIAAVFRNPYIRESVDELLENRDGLILGIGNGF
                     QALVRLGLLPYGTIKEPDEKDPILTLNTVGRHVSVISGTKVVSTKSPWLWNTKAGDVF
                     YLPVSCKEGRFHADEEAIRVLIENNQIATQYVDELGNVTMDPEWNKIGSMYAIEGITS
                     PDGRILGKMAHSERIGKNVCVNVPGPKDQMIFRSGVEYYTK"
     gene            2713..>3427
                     /locus_tag="PLI11_06860"
     CDS             2713..>3427
                     /locus_tag="PLI11_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPZ52621.1"
                     /translation="MNVMDLYKQWSTGISFDLETKAELLAIKDNPDEIRDRFYKELEF
                     GTAGVRGLVGAGTNRMNVYVVRKLSNAIAQYVMSLGIEKPTVMIGYDSRNHSLEFAKE
                     AAAVFAGNGIKVYMHSELCPVPAMSYSIRLLKCTMGVLITASHNPKEYNGYKVFGAVG
                     SQLAPDDIEKIKEILDTLTDYSKIPGFDFEYFSNRGKITKIGVNIKNSYLKTVRRLVI
                     DRNALSINAPKLKLVYTPLH"
BASE COUNT         1110 a          576 c          832 g          909 t
ORIGIN      
        1 tttgtcaggc agatcttatg tatatcaggc catgaggata acaggctgtt cagatccgac
       61 agtgcctgtt gaagagacaa gggaaggcaa actgccacaa agagttataa ccaccactgc
      121 cgcacaaggg tacagttcct atggcaatca aattggagta ccagccggct tcctcaagga
      181 aatatatcac ccaggttaca ttgccaaaag aatggaactt ggagcagtaa ttgctgcggc
      241 tccgcaagag aatgttgtca ggtccaagcc agagaaggga gatttaatca ttctcttggg
      301 aggaaggact ggaagagatg gtattggtgg agcaaccggt tcatctaaag aacagactgt
      361 cgatgcaatt caaacgcttg gctcagaagt gcagaaagga aatcctcctc ttgagagaaa
      421 cataatgaga ttgtttagaa agaaagaggt aagtacctta attaagaaat gtaatgactt
      481 tggagcaggt ggagtttctg ttgcaatagg tgagcttgct ccctctattt caataaatct
      541 tgatttggtt cctttgaaat atgatggtct tgatggaaca gagattgcca tcagcgaatc
      601 tcaagagaga atggctgttg tggtaagtga atatgatgcg gagaaattta ttcagtacgc
      661 taatgaggag aatctggaag ctgttgttgt cgcagagatt acggataccg gaagattgaa
      721 aatgtactgg cggggcgatt tgatagttga tatcagaaga gacttccttg atacaaacgg
      781 agttactcaa aaggcttgcg caaatgttga accttttgat ttgtatatag aaaaaaggaa
      841 cgctgaaagt ataaaggatg aatgggtaaa aaatctaatg aatttgaata gctgcagtca
      901 aaagggactt gtggagatgt ttgacagcac tgttggagga aatgctgtga ttatgcctct
      961 tgggggaaaa tatcagctca caccggcaga cggaatggta tcaaaaattc ctgttcttaa
     1021 tggagaaacc aaaaccggtt ccataatgac ttttggatac gacccttatt tatcagaaaa
     1081 gagtccattc catggaagca tgtttgctgt catagattca cttgccaagg tgaccagtct
     1141 tggtggagat ttctcaaaag caagacttac attccaggaa tactttgaaa gactctatga
     1201 tgttccaaca aaatggggaa aacctcttgc cgctctgctg ggagctttcc atgtacaaaa
     1261 agagttgaaa atcccttcaa ttggcggaaa ggattccatg agtggaacct tcatggaaat
     1321 tgacgtacct ccaacacttg tatcatttgc ggtgtcggta ctggaggatg ttggtaaagt
     1381 agtaacgcag gaattcaaaa aggcaaactc tagagtaatt ctttacaaac tccctgttaa
     1441 cgaagaattc atgcctgatt ttgaaagctt gaaaaagaac tatatgaaaa tacacacgct
     1501 agcagatcaa ggcaggataa aagcagccat ggcagtcagg tttggcggga ttgcagaaac
     1561 aatatccctt atgtgctttg gcaacatgat tggctttgat ttgaaaaact ttgacaatct
     1621 ctttgataaa atgtacggtt cgattataat tgagttggat gacagtgcaa gcgataagga
     1681 ccttgaaggc cttgactata ttgatttggg aactactatt gaatacaagg tagttagact
     1741 gggagatgaa attgtttcac tagacgagct tttggaaaac tggagaaaac cgcttgacga
     1801 tattttccct gagaaggcgt cgtttgtgaa taatgatgta aatattcccg aatacgatga
     1861 aaaggataga acatattcaa gtataaaggt tgccaaacca aaagtgttca tacctttgtt
     1921 ccctggaaca aatggtgaat atgagacaat caaggctttt gaagatgctg gcgctgttgt
     1981 agagacattt gttgttagaa acaggtcagg caaagaaatc gaagaatcaa tcaaagttat
     2041 aaagagtatg attgaaaatt cgcagattgt tgcactgcct ggaggaagta gtgctggaga
     2101 cgaacctgag ggagccggca aatttatagc ggcagtattc agaaatccat acattagaga
     2161 gagcgtggat gagttattgg aaaatcgtga cggtctgatt cttggaatag gcaacggatt
     2221 ccaggctctt gttagacttg gactgctccc ttatggtaca ataaaggagc ctgatgaaaa
     2281 agatccaata ttgacgctta atacggtagg cagacatgta tccgtgattt caggaacaaa
     2341 ggttgtatcc acaaagtcgc cttggctttg gaatacaaaa gcaggagatg ttttttacct
     2401 acctgttagc tgtaaagaag ggcgttttca tgcggatgaa gaggccatca gggttttgat
     2461 tgagaacaac cagatagcaa cccaatatgt tgacgaactt ggaaatgtta ctatggatcc
     2521 tgaatggaac aagattggaa gcatgtacgc aatagaaggt attacaagtc ctgatggaag
     2581 aatccttgga aagatggccc attctgaaag aataggcaaa aatgtttgtg tcaatgttcc
     2641 aggacctaaa gaccagatga tattcaggtc cggggttgaa tattatacaa aataatacaa
     2701 gggggttaac ttatgaatgt aatggatttg tataagcaat ggagtacagg tatttcgttt
     2761 gatttggaaa ctaaagccga gcttttagct attaaagaca atccagatga gatcagagac
     2821 cggttctata aagaactgga gtttggaacc gcaggggttc gaggcttggt tggcgcaggc
     2881 actaaccgaa tgaatgtcta tgtggttcga aaactcagca atgccatagc tcagtatgta
     2941 atgagccttg gtatcgaaaa accgaccgtt atgattggtt atgattcaag aaaccactca
     3001 ctggaatttg ctaaagaagc tgcggcggta tttgcaggca atggcataaa agtctacatg
     3061 cacagcgaat tatgtccagt acctgccatg tcgtactcaa tcagattgct gaaatgtaca
     3121 atgggcgttt taataacggc aagtcataat cccaaggaat acaacggata caaggtgttt
     3181 ggtgcggtgg gctcacaact ggctccggat gatattgaga agataaaaga aattttggac
     3241 actcttactg attacagcaa gattccagga tttgatttcg agtacttttc caatagaggc
     3301 aaaattacca aaattggagt gaatatcaaa aactcttatc taaaaacagt aagaaggctt
     3361 gtcattgaca ggaatgctct cagcatcaat gctccaaagt tgaagttggt ctacactcca
     3421 ttacatg
//
LOCUS       DAONVY010000235         3420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19151_length_3420_cov_3.509064, whole genome shotgun sequence.
ACCESSION   DAONVY010000235 DAONVY010000000
VERSION     DAONVY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19151_length_3420_cov_3.509064"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..940
                     /locus_tag="PLI11_06865"
     CDS             119..940
                     /locus_tag="PLI11_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPZ52622.1"
                     /translation="MKALTDYRISALNINMFLHGIKKTRRNVQGGEFDSLREYVQSDT
                     YNIINWAATARRHELIVNTYVPEKNQYIFIMIDSSRVMNSEYKNIKKLDYAINSAFLL
                     ADYCTHGGDNVGLMVFDNKVRRYVAPRKNNFETIASNLYNVEYTETSADYDNAIKTFK
                     LKQRRRTLVFIFTELFNVDEALRFANAVNTHLSNHLVYAITIKDPRVDDIANMDIEKL
                     DDVYLKSAGVKLVGERDKIRAVLKKNGIMSSDVEPDKLSLEAVCRYLDIKRSGAL"
     gene            complement(942..1799)
                     /locus_tag="PLI11_06870"
     CDS             complement(942..1799)
                     /locus_tag="PLI11_06870"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPZ52623.1"
                     /translation="MTLKKNVYIETHENVTIEYETANLGSRFLARLLDEVIVYAALII
                     TVIILSYTVFENSSLNLSNIVDSWNILYVIAIVYIVTFILKYFYFVIFEMTMRGQSPG
                     KKAAGLRVVSTTGEPIIFSASLVRNLLRIVDDLPANYLVGAVFIMFNKKSQRLGDIAA
                     NTMVIKTKENTKFTKQLEQMLQDEINKEVDMQEVLDKYLESSENIKSPDETIPEETNA
                     TISKHEYEILSEYLAQREFIHDSNIWDVKLFNYLFRRVNAQIPQRMYYAYVIQFLIGV
                     ERYNRQFYT"
     gene            1896..2888
                     /locus_tag="PLI11_06875"
     CDS             1896..2888
                     /locus_tag="PLI11_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPZ52624.1"
                     /translation="MNEQEFVKANGNAWKELESELKLFRKISEKQNIDKHRIDRFVTL
                     YNKAANNLAYSRTYFGECATTNYLNRLVGQAHSFIYSSPESKVSSNFKIFTTGFPKLL
                     RRYIKAFVIATLIFVVAFAFSYIVTAISLDNALLFLPRQVLEGSRQEGQYDEFTTSIG
                     TYFASMIGTNNLRVSFMAFALGITFGIGTIYILAYNGLMLGALAGVYLHRGQSVFFWS
                     LILPHGVSELFAIFVSGTAGLIIGYSLINPGKISRKDALITKGMDAVKLIVGCIPILV
                     ISALIEGFFTPLELPYIYKYVFSLTMLCLLILYCVLPGSEREPKEQEKILEKLV"
     gene            2980..>3420
                     /locus_tag="PLI11_06880"
     CDS             2980..>3420
                     /locus_tag="PLI11_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HPZ52625.1"
                     /translation="MAIKVGINGFGRIGRLVFRLATQNPEIEVVGINDPFLDPDYMAY
                     MVKFDTVHGRFDGTAEAVDGKLVVNGKEIEVFACMDAKEIPWGKVGADYIVESTGVYT
                     TIDKAKAHLEAGAKKVVITAPSKDAPMFVMGVNSDKYTKDMEIVS"
BASE COUNT         1010 a          599 c          762 g         1049 t
ORIGIN      
        1 catgaaaagg ggagaggtgt gttttgagta tatatatata agatatttgg ggatgtatgg
       61 atttattcaa aggcagaggt gaataaaaag tgatgagaaa tacaaggttt accccaacat
      121 gaaagcgctt accgattacc ggatcagcgc tcttaacatt aacatgtttc tccatggcat
      181 aaagaagacc agaagaaatg tgcaaggtgg cgagtttgac agcttgaggg aatatgtaca
      241 gtcggatacg tacaatatta tcaactgggc ggctaccgca agaagacacg agcttattgt
      301 aaatacttat gttcctgaaa agaatcagta tatttttatc atgatagatt ccagcagggt
      361 catgaattca gagtataaaa acataaagaa gctggattat gcaataaaca gtgcttttct
      421 gttggctgat tactgcacgc atggcggaga caatgtgggt cttatggtgt ttgacaacaa
      481 ggtaagaagg tatgttgctc ccagaaagaa caacttcgaa accattgctt cgaatttgta
      541 taatgtggag tatacggaga ccagtgcgga ttatgacaat gccattaaaa ccttcaagct
      601 aaagcagaga aggagaaccc ttgtttttat tttcacagag cttttcaatg tggatgaagc
      661 attaagattt gcaaatgccg taaatactca tctttcaaat catttggtat atgccataac
      721 cattaaagac ccaagagtgg atgacattgc gaatatggat attgagaagt tggatgatgt
      781 atacctgaag tccgctggtg tcaagttggt tggtgaaaga gacaagataa gagccgtatt
      841 aaagaaaaat ggaataatga gctccgatgt ggagcccgac aagctatcct tggaagcggt
      901 ttgcagatat ctggatataa agagatccgg ggctctgtga gttacgtata gaactgcctg
      961 ttataccttt caacgcctat caggaattgg attacatatg cgtaatacat tctttgcggt
     1021 atctgggcat tgactcttct gaagaggtag ttgaacagtt tcacatccca tatgtttgaa
     1081 tcgtgtatga attccctctg agcaagatac tcggatagaa tctcatattc atgctttgag
     1141 attgtggcgt ttgtttcttc cggtattgtt tcgtcagggg atttaatgtt ttcggatgac
     1201 tcgagatact tgtccaaaac ttcctgcatg tctacttctt tgttgatttc atcctgaagc
     1261 atttgttcaa gttgttttgt gaactttgta ttttccttgg tttttataac catggtgttg
     1321 gcggcaatgt cgccaagtct ttgggatttt ttattgaaca ttatgaatac ggcacctacc
     1381 aggtagtttg caggtaaatc atccacaatc ctgagcagat tgcgaaccaa ggaagcagag
     1441 aatataattg gctcgcctgt tgtggataca actctcaaac ccgctgcctt tttccctgga
     1501 gactggcccc tcattgtcat ttcaaaaatt acaaagtaga aatattttag tatgaaagta
     1561 acaatgtaca ctatggcgat aacgtataag atgttccagc tgtcaactat attggacagg
     1621 tttagactac tgttttcaaa aactgtgtat gacaggataa ttaccgtgat tatcaatgcg
     1681 gcatagacaa tgacttcatc gagtaggcgg gcaagaaacc tggagcccag gttcgctgtc
     1741 tcatattcta tggttacatt ctcgtgggtt tctatgtaga catttttctt caaagtcata
     1801 ttaccttcct tgtttttgtt gataccttcc ataattaatg ttattatatt agcacaccca
     1861 aaattttata tcaatatcat aaagaggcga atcatatgaa tgagcaggag ttcgttaaag
     1921 ccaacggaaa tgcatggaaa gagcttgaat cggagttgaa gcttttcaga aagatatctg
     1981 aaaagcaaaa cattgacaaa cacagaatcg acaggtttgt cacgctttac aacaaggcgg
     2041 ccaacaattt ggcatactca aggacatatt ttggagaatg tgccacaacc aattacctta
     2101 acaggctggt tgggcaggct cactccttca tatattcgtc accagaatca aaggtatcgt
     2161 ccaattttaa gatatttacc acaggttttc ccaagctgct taggaggtat ataaaagcat
     2221 ttgttattgc tacgctgatt tttgttgttg catttgcttt ttcctatatt gtgactgcaa
     2281 taagtctgga caatgccttg ctgttcctgc caaggcaggt tctggaaggt tcaagacagg
     2341 aaggacaata tgacgagttt accacatcta ttggcaccta tttcgcatcc atgataggca
     2401 cgaacaattt gagggtaagt ttcatggctt ttgctttggg tataactttt ggaataggca
     2461 ccatttacat acttgcctac aatggtctta tgctgggagc attggcagga gtttacctgc
     2521 atcggggaca gtcggtattc ttctggtcgt tgatactgcc tcacggtgtg tctgaacttt
     2581 ttgcaatctt tgtcagcggg actgcaggac ttattattgg ttactctctt attaatccgg
     2641 gcaagatatc caggaaagat gccctgatta caaaaggtat ggatgcggtg aaacttatag
     2701 tcggctgcat acccatactg gtaatatccg cattgataga aggcttcttt acacctcttg
     2761 aactgccata tatttacaaa tatgtttttt cactgaccat gctttgcctt cttattcttt
     2821 actgtgtgct tccaggcagt gaaagggaac cgaaggaaca ggagaaaata cttgaaaaac
     2881 ttgtctgatt atatattgtt ttttatgaca aattaaggta tactaatagt tggtggtgca
     2941 aaaaagcact gtaaatttaa taaaaatgga ggttttttta tggctattaa agtaggtatt
     3001 aatggtttcg gtcgaattgg tcgtcttgtg tttagacttg ctacacagaa tcctgaaatt
     3061 gaagtagtag gaatcaacga tcctttcctc gatccggatt atatggctta tatggttaaa
     3121 tttgatactg tacacggtcg tttcgatgga acagcagagg ctgttgatgg caaactcgta
     3181 gttaatggaa aagaaataga agtgtttgca tgcatggatg caaaagagat tccatggggc
     3241 aaggtaggag ctgattacat tgtagagtcc actggtgttt atacaaccat tgacaaggca
     3301 aaggctcacc ttgaagctgg agctaagaag gttgttatca ctgctccatc caaggacgct
     3361 ccaatgttcg ttatgggtgt aaatagcgac aagtacacaa aggatatgga aatcgtttcc
//
LOCUS       DAONVY010000236         3414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19192_length_3414_cov_3.370944, whole genome shotgun sequence.
ACCESSION   DAONVY010000236 DAONVY010000000
VERSION     DAONVY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19192_length_3414_cov_3.370944"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLI11_06885"
     CDS             complement(<1..633)
                     /locus_tag="PLI11_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52626.1"
                     /translation="MSKIMKLDDYIKSREMKNKATYKYSNKQEVKKKWVELCKRLNSG
                     KQVSDYFTLVIELLSLLHYVLSDILDDRYIESDFMKNFEELSKKGYSFDRTTLNRLNR
                     IRIIRNLSAHNTSTIMTESVNYELCVEIFLVIGKVLYELKILDKDDIQPSPEKLQAEV
                     NDIVGGYCRLDELIGSGGGGRVFKAYHINLDIYVAVKEINYELKDKIYTEN"
     gene            complement(652..1572)
                     /locus_tag="PLI11_06890"
     CDS             complement(652..1572)
                     /locus_tag="PLI11_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPZ52627.1"
                     /translation="MKKDMSLMLQLIAIIANNRGITTKQLANILNVPEGVVLRNLELI
                     SYNPEFRLLDIYSEEHEDAIMDLGDMTWNIACEDEEDSDDIVYLCINSVEKFLFESLV
                     ANAFFDDEILGIRIKSTHGLAEQKDKFLIITLAIENRKKLKVKYKTRYKTSDFLIEPL
                     RIVYYEIDNMVYIIGQHNGRIATYRLDRMIEIKATNERFEPIGVEHVESLLEYSWGME
                     QGNLFNVKVKFLDEANVIYKVKRDLSHRKNKKITEYEGYIIYEDTIAGINSFKRWLRS
                     YGSSAIVLEPEELRNDMIRSAEQCLSYYIE"
     gene            complement(1520..2365)
                     /locus_tag="PLI11_06895"
     CDS             complement(1520..2365)
                     /locus_tag="PLI11_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPZ52628.1"
                     /translation="MIYIDENNKCCLSATSDFLYKTDDVNLYAFLELSNLYKHHLPLN
                     DILHSLKSKIITQTMPYEDEYDIYSIGRRYRISKHLLRNIDEFETCDYRRKSLEITYN
                     SKVGKLSVIVQVITLFYHYDNDKSYVIGKVKDEIFFIDISSIIEIKSTNIRNECYKDD
                     NVMQKLELMFGASLDGPFDVVVEFDNKFNIMERLTRLKRLRKSCSLSVFGETIIYKDT
                     IYGMHDFARFLRSFGSSCRVISPNELKDLMRNTYERILKSYGVGIKYEERHEPDAATY
                     SYYCK"
     gene            complement(2386..2745)
                     /locus_tag="PLI11_06900"
     CDS             complement(2386..2745)
                     /locus_tag="PLI11_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPZ52629.1"
                     /translation="MSVKEQKINKLIHHFLTHNTITFDEIKMLLDISNRSVSYYIKDL
                     RKLGYNIRRKNNNYVLVEDDYEKGMLLSNTDLRVIKILLTVGECNGRLEHRELVEMLK
                     ESLCDADVSVKRPLLGR"
     gene            complement(2941..>3414)
                     /locus_tag="PLI11_06905"
     CDS             complement(2941..>3414)
                     /locus_tag="PLI11_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPZ52630.1"
                     /translation="AITLKEDLDPSTLDPESEVNASLTLEDGTMTFQIGANKDQSIDL
                     SIGDMSAEALGVAYVSVSSNTDADSAIDTIDKAINKVSEERAKLGAYQNRLEHTINNL
                     NTSAENLTAAESRIRDVDMANEMMEYTKNNILAQAATAMLAQANQAPQSVLQLLQ"
BASE COUNT          985 a          730 c          499 g         1200 t
ORIGIN      
        1 gttctcagta taaatcttat ctttcagttc atagttaatt tcttttacag caacatatat
       61 atcaagattg atatgatatg ccttaaacac tctcccgccg ccaccgcttc caatcagttc
      121 atcaagacga caataacctc caacaatatc gttgacttct gcctgtaatt tctcaggcga
      181 cggctgtatg tcatccttgt ccaatatttt aagttcataa agaacttttc caataaccaa
      241 aaaaatctca acacacaact catagtttac actctcagtc attatcgttg atgtattatg
      301 agcactcaaa ttacgtatga ttcttatcct atttagccta tttagggttg ttctatcaaa
      361 gctgtatcct tttttactta attcctcaaa gtttttcata aaatcagact caatataacg
      421 gtcatctaat atgtctgata aaacataatg taacaagctt agcaactcaa ttactaaagt
      481 aaaataatca ctaacctgtt tcccggaatt tagtctttta cagagttcaa cccacttctt
      541 ttttacttcc tgcttgttac tgtatttgta ggtcgcttta ttcttcattt ccctagattt
      601 tatataatcg tccagtttca ttattttgct catctgactg cccctattca tttattctat
      661 atagtatgac aagcattgtt ctgcagaccg tatcatatcg tttcttaatt cttcgggctc
      721 aagcacaatt gcagatgaac cataactcct aagccacctt ttgaagctgt ttatgcctgc
      781 aattgtatcc tcatatatta tgtaaccctc atattctgtt atcttcttgt ttttcctgtg
      841 ggacaaatcc cttttaacct tataaattac atttgcctcg tccaaaaact ttacttttac
      901 gttgaataga ttaccttgtt ccatacccca gctatactca agaagactct cgacatgctc
      961 cactccaata ggctcgaacc tttcattagt tgctttaatc tctatcattc tgtcaagcct
     1021 gtacgttgca attcttccat tgtgctgtcc tatgatgtaa accatgttat ctatttcata
     1081 atatacaatc ctcaacggtt ctattaaaaa atcgcttgtt ttatatcttg tcttgtactt
     1141 tacctttagc ttcttcctat tctctatagc tagtgtgata atcaggaact tgtccttttg
     1201 ttcagctaaa ccatgtgtag actttatcct aataccaagg atctcatcat caaaaaacgc
     1261 atttgcaaca agactttcaa ataggaactt ctccactgag ttaatgcaca aataaacaat
     1321 atcatcactg tcctcttcat cctcgcatgc aatattccat gtcatatcac ccaaatccat
     1381 tattgcgtct tcatgttcct cactgtaaat atcaagaagt ctgaactcag ggttgtatga
     1441 tatgagctca agattgcgca agacaacacc ctcaggtaca ttcaagatat ttgccagctg
     1501 ttttgtcgta attcccctgt tatttgcaat aatagctata agttgcagca tcaggctcat
     1561 gtctttcttc atatttgata cctaccccgt aactttttaa aattctttca tatgtatttc
     1621 gcattaagtc tttcaattca tttggggaga tgaccctgca ggaagagcca aagctccgca
     1681 aaaaacgagc aaagtcatgc atcccataaa ttgtatcttt ataaattatt gtctccccga
     1741 aaactgacaa actacagctt tttcttaatc ttttcagcct tgttaatctt tccataatgt
     1801 taaacttgtt atcaaattca acaacaacat caaacgggcc gtcgagcgat gctccaaaca
     1861 ttaactctaa tttttgcatc acattgtcgt ccttgtagca ctcatttctt atgtttgtag
     1921 attttatttc aataatagaa ctaatatcaa taaagaagat ttcgtcttta accttaccga
     1981 taacataact tttatcgtta tcgtaatggt agaacagagt aataacctga acgattacgg
     2041 atagttttcc tacctttgag ttatatgtta tttctaaaga ctttcttcta taatcacagg
     2101 tttcaaactc atcaatattt ctcaaaagat gtttgcttat acgatatctt ctaccgatag
     2161 aatatatgtc gtattcatcc tcataaggca ttgtttgagt tattatttta cttttcaggg
     2221 agtgaagaat gtcgttgagc ggaagatgat gcttgtacaa gtttgagagt tctaaaaagg
     2281 cataaagatt aacatcatct gttttataca gaaaatcaga cgtagcagac agacagcatt
     2341 tgttgttctc atcaatgtat atcaaattgt tttcaacaca tagttctatc gccctaataa
     2401 tggtcttttc actgaaacat cagcgtcgca cagactttct ttaagcattt caacaagttc
     2461 tctgtgttca agtctgccgt tacactcacc aacggtcaac aaaattttaa taactctcaa
     2521 atcagtgttg ctaagaagca ttcctttttc gtaatcgtct tcaaccaaca cgtaattatt
     2581 gttttttctc cttatgttgt atcccagttt tctaagatcc tttatataat agcttacact
     2641 gcgattgcta atatccaaca acatcttaat ttcatcgaaa gtaatcgtat tgtgtgtcaa
     2701 aaaatggtgt atcaacttgt ttattttttg ctcctttacg ctcattaaat tcctccgaat
     2761 acctccacat caatattagg ttatttttaa gttattggta ttatacacca taatcctagt
     2821 cagtcaagct tctgagctat acaaaatgta acacaaatat agcatgtgaa taaaaattgg
     2881 agcataataa aaggaatgga accaattagc tccattcctt gattactata cactagttac
     2941 ttattgaagc agctgcagta ctgactgtgg tgcctggttg gcctgagcca gcattgctgt
     3001 agcagcctgt gccaggatgt tgttcttggt gtactccatc atttcgttag ccatgtcaac
     3061 atcacgaata cggctttcag ctgcagtaag attttcagct gatgtattca ggttgttgat
     3121 ggtatgctca agacggttct ggtatgcacc aagtttagct ctttcctcag aaaccttgtt
     3181 tattgccttg tctatggtgt caatcgctga gtctgcatca gtgttgctgc ttacagaaac
     3241 ataagcaaca cccagagcct ccgctgacat gtcaccaata gaaagatcta tgctctgatc
     3301 cttattggca ccaatctgga atgtcatggt accatcttct aacgtaagtg aggcattaac
     3361 ttctgattct ggatcgagag tagatggatc aaggtcttcc ttgagtgtaa ttgc
//
LOCUS       DAONVY010000237         3405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19266_length_3405_cov_3.541194, whole genome shotgun sequence.
ACCESSION   DAONVY010000237 DAONVY010000000
VERSION     DAONVY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19266_length_3405_cov_3.541194"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PLI11_06910"
     CDS             complement(<1..160)
                     /locus_tag="PLI11_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPZ52631.1"
                     /translation="MGDRKSRRKRSGSSDNWQTTYGDMVTLLLTFFVLLFSFSTINEA
                     KWKELVFAF"
     gene            complement(153..956)
                     /locus_tag="PLI11_06915"
     CDS             complement(153..956)
                     /locus_tag="PLI11_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPZ52632.1"
                     /translation="MDIMGILGIILGFLCMVIGIMINGTLGSYWDSASIFITLGGTIF
                     AAMASVRFDTFKNLFKLVGIALRRDDTDFTGSIQTLIHLANVARKDGVLALEGEAENL
                     DDNFLKKGLLLVVDGSDPELVKNILQTEINFIEERHEQGQSMFKSMASYAPAFGMAGT
                     LIGLINMLQELDKPETLGPAMAVALITTFYGVLLANLWFMPLARRLEDKTIIEISHKE
                     MIIEGVLSIQAGENPRIIEEKLKAFLSQEEQENVEVNKRFNVAGEMQDG"
     gene            complement(970..1176)
                     /locus_tag="PLI11_06920"
     CDS             complement(970..1176)
                     /locus_tag="PLI11_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HPZ52633.1"
                     /translation="MIKVTRINNTVLYVNPDLIEFFEETPDTVITMTTGKKLIVLESV
                     EELQERIIAYRKEVYAGLPKKVTR"
     gene            complement(1189..2007)
                     /locus_tag="PLI11_06925"
     CDS             complement(1189..2007)
                     /locus_tag="PLI11_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019714413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HPZ52634.1"
                     /translation="MMRSMFSAVSGLKNHQVKMDVIGNNIANVNTVAYKSGRVTFQDI
                     FNQTLKPASGADVNLGGINPQQVGLGVSIGTIDTMFTSAGAQRTDYALDLSIEGDGFF
                     VVQNGAGETFYTRAGNFRLDDQGNLVNANGMFVLDVDGNAINLGGVQYHDISIDYMGR
                     MMGIPSGSSTSQVIAVLGIATFTNTSGLQKAGNNTYIETTSSGSPLPGSTSVYKQAGF
                     DGAGALNPGTLEMSNVDLSNEFTDMIITQRGYQANARVITVADSLLEELVNLKR"
     gene            complement(2022..2411)
                     /locus_tag="PLI11_06930"
     CDS             complement(2022..2411)
                     /locus_tag="PLI11_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPZ52635.1"
                     /translation="MKVDNVASQMTLQNNNVREARKDLGKDDFLKLLVTQLQNQDPLS
                     PMEDTDFIAQMAQFSALEEMSALNKSFEFGSSVSLIGKEVIAKYGNDLIQGVVERVSV
                     QSGQSWLTVGEYMFQLSDVVEVVYDNN"
     gene            complement(2423..>3405)
                     /locus_tag="PLI11_06935"
     CDS             complement(2423..>3405)
                     /locus_tag="PLI11_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPZ52636.1"
                     /translation="AKYMVPMFVPVNHENQIPVMTEEQPERQWGELEEVNVNTVELDI
                     FPRDVKVQDFDVEVSTKDKTFDDLLFIEDNETEVFDMKTEALDVRQEPKEVFAVDIRE
                     GRWNVTESHTIEEKLDISNKGVEKNDTETYSALKAVNKLSIERPKEIRLQRDKEYPNV
                     ERFDIGIPQNALEVENENLSVSRSHVVESMNRITETIVESISRRETEDNVELTIKLKP
                     EFLGEVEITLEESNGKVKAFLKISNEDLRDALNQRVVEIETALRNQGIEMDKIEFGSF
                     AFQFDTESTGGQGRQGSYKQPSFGYHAERKDALDEESNVFHASGRINYLV"
BASE COUNT          872 a          854 c          592 g         1087 t
ORIGIN      
        1 taaaagcaaa caccaattcc ttccatttgg cctcatttat ggtcgaaaag gagaacagga
       61 ggacgaagaa ggtaagcagg agtgtaacca tgtccccgta tgtagtctgc cagttgtcag
      121 agctgcctga tctttttctt ctcgactttc tatcacccat cctgcatctc ccccgctaca
      181 ttaaaccttt tattaacttc cacgttttcc tgctcctcct gggataagaa cgcctttagt
      241 ttctcttcga ttattctcgg gttttctcca gcctgtattg ataaaacccc ttcaataatc
      301 atttccttgt ggcttatctc gattattgtc ttgtcctcaa gccttcttgc aagaggcata
      361 aaccacaagt tggcgagaag cactccatag aaagtagtaa taagtgccac agccatcgca
      421 ggtcccaacg tctctggctt gtccaattcc tgaagcatgt tgataagccc tataagggta
      481 cctgccatac cgaaagcagg agcataggac gccatgctct tgaacatgga ctgtccttgt
      541 tcatgccttt cttcaataaa gtttatttca gtctgtagaa tattctttac aagctcagga
      601 tctgaaccgt ccactaccaa aagcaaacct ttcttaagaa agttgtcatc aaggttttcc
      661 gcctcccctt caagagcgag tacgccatcc tttcgggcaa cgttggccag atgaataagt
      721 gtttggatac tgcctgtaaa atccgtgtca tcccttctaa gagcgattcc aaccagtttg
      781 aaaaggtttt taaacgtatc gaagcgcaca cttgccatgg cagcaaatat ggttccgccc
      841 aatgttatga aaatagaggc agagtcccaa tatgagccta gtgtcccatt tatcattatt
      901 cctataacca tgcatagaaa acccaaaata atacctagta tacccattat gtccataaaa
      961 tcaccattcc taccttgtca ctttcttagg gaggcctgcg taaacttcct ttctgtatgc
     1021 aatgattctt tcctgcagtt cttcaacgga ttccaatact atcagtttct tgcctgtagt
     1081 catggttatg acagtatcgg gagtttcctc aaaaaactca atcaaatccg gattcacata
     1141 caagacagta ttgtttattc ttgttacctt aatcattttt agttaacctt agcgtttgag
     1201 atttacaagt tcttcaagca gcgagtcagc tacagttatg actctcgcgt tagcctggta
     1261 acccctctga gtaataatca tgtcagtgaa ctcgttggat aaatccacat tggacatctc
     1321 caaagttcct ggattcaatg cacctgcacc gtcaaaacca gcctgcttgt aaacggatgt
     1381 gcttcctgga agtggggagc ctgagcttgt agtttcaata tatgtattgt tgccagcctt
     1441 ttgcagtcct gatgtgtttg taaaggtggc gataccaagt acggcaataa cctgggatgt
     1501 gcttgaaccg ctggggatac ccatcattct tcccatatag tctatggaaa tgtcatggta
     1561 ttgtactcca cccaggttta ttgcgtttcc gtcaacatca agaacaaaca taccattggc
     1621 attcacaaga tttccctggt cgtcaagtct gaagttgccc gctcttgtgt agaaggtctc
     1681 tcctgctccg ttttgtacaa caaagaaacc atcgccttca atagaaaggt caagggcata
     1741 gtcggttctc tgcgcacctg cggaggtaaa cattgtatct attgttccaa tacttacacc
     1801 aagacctacc tgctgtggat ttattccacc aaggttgaca tctgctccgc tggcaggttt
     1861 caaagtctgg ttgaatatgt cctgaaatgt aacccttccc gatttatatg ccaccgtatt
     1921 tacattagct atgttattac ctattacgtc cattttaacc tgatggttct ttagtccgga
     1981 aactgctgaa aacattgatc tcatcataat gaattcctcc tttaattgtt atcgtataca
     2041 acttccacca catcggaaag ttggaacata tattctccta cagtaagcca gctttgaccg
     2101 ctttgtaccg agactctttc aacaacaccc tgaatcaggt cattgccgta ttttgctatt
     2161 acttccttgc caatcaggga aacgctgctt ccaaactcaa acgatttgtt caatgcactc
     2221 atttcctcaa gggcgctgaa ctgagccatt tgtgctatga aatcggtatc ctccatggga
     2281 cttaaaggat cctgattctg cagctgggtc acaagaagct ttaaaaagtc atcctttcca
     2341 agatcctttc ttgcttccct gacattatta ttctgtaaag tcatttgact tgccacattg
     2401 tcaaccttca ttttcctacc tcctacacca agtaattgat tcgtccgctg gcatggaaga
     2461 cattggactc ttcatcaagg gcatccttcc tctcagcatg ataaccaaaa gaaggttgtt
     2521 tgtaactgcc ctgccttccc tgtccgcctg tgctttcagt atcaaactgg aacgcaaacg
     2581 aaccaaattc aatcttgtcc atctcaattc cctgatttct caaagctgtt tctatctcca
     2641 caactctttg gttcaaggca tcccgcagat cctcgttgga tatcttgaga aaagccttta
     2701 ccttgccatt tgattcctcc agtgtgattt ccacttctcc aagaaactcg ggtttcagtt
     2761 tgattgtaag ttcaacgtta tcttccgtct ccctccggct tatggactcc actattgtct
     2821 ccgttattct attcatgctc tctacaacat gacttcttga tacagacaaa ttctcatttt
     2881 ccacctcaag ggcgttttgt ggaattccaa tatcaaacct ctcaacattt ggatactctt
     2941 tgtccctttg aagcctgatt tcctttggtc tttcaatact taacttattg acggctttaa
     3001 gtgcagaata cgtttcagta tcattctttt ccacaccttt attcgatata tccaactttt
     3061 cttcaattgt atgtgactct gtcacattcc atctgccctc cctgatgtca actgcgaaaa
     3121 cttcttttgg ttcctgcctg acatccaggg cttcggtctt catgtcaaaa acttctgtct
     3181 cattgtcttc aataaataac aagtcatcaa aagttttatc ttttgtactt acttcaacgt
     3241 caaaatcctg cacctttaca tccctaggga aaatatcaag ttctactgta ttgacattga
     3301 cttcttccag ttcaccccat tgcctttccg gttgttcctc tgtcatcaca ggtatttggt
     3361 tttcatgatt gacaggaaca aacatgggca ccatgtactt tgcca
//
LOCUS       DAONVY010000238         3401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19300_length_3401_cov_3.764196, whole genome shotgun sequence.
ACCESSION   DAONVY010000238 DAONVY010000000
VERSION     DAONVY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19300_length_3401_cov_3.764196"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLI11_06940"
     CDS             <1..776
                     /locus_tag="PLI11_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52637.1"
                     /translation="VIEAFSGKIRSFDRDMFNDNYQKKPEKTAAPEKKELDKIEEKPK
                     PELKPMPEPKPLVDIETKTEIKEEKPPEPQKTANDTTNGRTIAPSKTEQFTKPQNMEK
                     PSFDWDEFLNKELKAADINKEVKKEEKYPDDTFNEMLLPFDPFNTTNKAYNWWVADNY
                     QQASDAFKAINVLMPTIITKFLYNNIENHKHALIGRYEQDGRKFVILGIPSANVITNS
                     VARWMKCSVFYTDTEYVGYLLYYIDAKTSSLVKAVPQED"
     gene            complement(779..1303)
                     /locus_tag="PLI11_06945"
     CDS             complement(779..1303)
                     /locus_tag="PLI11_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HPZ52638.1"
                     /translation="MTQFISYVIPVMVLLILVSGVFRKVKCFDSFIVGAKEGATTIVK
                     LLPSLVGLIVAISVFRASGALDILVSFLHPVTSFLGLDDALVPFLIMRPVSGSGSLAL
                     LADIFKTYGADSKAGMIASVMMGSTETILYTLTVYTQNTRIRKTPNVLTAALVASFAS
                     AFYAGVFISLIFGI"
     gene            complement(1303..1881)
                     /locus_tag="PLI11_06950"
     CDS             complement(1303..1881)
                     /locus_tag="PLI11_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPZ52639.1"
                     /translation="MLNYIWVALLIVGLVVGIANGKIDAVSKTLLDASNDAVTFCIGL
                     LGVVSLWCGLMRIFQDAGGINFISKLISPVIRKLFPETRYNKEAEKHIVTNLTANFLG
                     LGNGATPSGIAAVHELERTNPNTTARKSTCLFLVVNSACLQLVPTTIIALRNSFGAKN
                     PADVLVPIWITSVLTLITALLVFFILDGRRKR"
     gene            2039..2278
                     /locus_tag="PLI11_06955"
     CDS             2039..2278
                     /locus_tag="PLI11_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPZ52640.1"
                     /translation="MKSTGIVRRVDELGRVVLPIELRRTLDIQEKDALEIYVDANSII
                     LKKYEPSCVFCGNARDVIQYKGKNICPDCIYDLKK"
     gene            complement(2297..3133)
                     /gene="rsmI"
                     /locus_tag="PLI11_06960"
     CDS             complement(2297..3133)
                     /gene="rsmI"
                     /locus_tag="PLI11_06960"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPZ52641.1"
                     /translation="MLYLIATPIGNLEDISIRALDTLRSVDLIAAEDTRHTAKLLMRF
                     DIRKPLVSYHEHNKDTLGEKLIEKLKNGESIAYVTDAGTPGISDPGEDLVKLAIENGI
                     EYTLIPGPCALIMGVVLSGFNTRSFVFEGFLPQDKKKRREVLKRISDEKRTLVFYEAP
                     HNILSTLKELKEHVVDRKICLARELTKKFEELKWGSVDSLIGFYEENNPRGEFVIVIE
                     GNREVEEVIEYEEVEIFNMVNEKIESGLKRNNAIKEVAKETGLSRNDVYAIYERKKGT
                     TL"
     gene            complement(3143..>3401)
                     /locus_tag="PLI11_06965"
     CDS             complement(3143..>3401)
                     /locus_tag="PLI11_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52642.1"
                     /translation="AQVLVPKGTLYMVNRPDRLCDTLVALRKYKLEPKLIRFVHSKLS
                     SSPILFLLKANLYGGENLKVGTPVLVDPNLSCKMIEEKVNL"
BASE COUNT         1087 a          755 c          619 g          940 t
ORIGIN      
        1 ccgtcattga ggctttttcc ggaaaaataa gaagctttga cagggacatg ttcaacgaca
       61 attaccaaaa gaagcctgaa aaaactgctg ctcctgagaa aaaggagttg gacaagatcg
      121 aagaaaaacc aaaaccagaa ctcaaaccca tgccagaacc aaaaccgctt gttgatattg
      181 aaaccaaaac cgagataaaa gaggaaaaac ctcccgagcc gcagaaaact gcaaacgaca
      241 caacaaacgg ccgtacaatt gcgccttcaa aaacagagca atttacaaaa ccgcagaaca
      301 tggaaaaacc cagctttgac tgggatgaat tcctgaacaa ggagctaaag gcagccgaca
      361 taaacaagga agtcaagaag gaagagaaat atcctgacga caccttcaat gaaatgctcc
      421 ttccctttga tccattcaat actaccaaca aggcttacaa ctggtgggtt gcagacaact
      481 atcagcaggc atccgacgcc ttcaaggcaa taaatgtact tatgcccaca ataataacaa
      541 agtttctgta caacaatatt gaaaaccaca aacatgcact gattggcaga tacgagcagg
      601 acggacggaa attcgtaata ctgggcatac cctctgccaa tgtcataacc aactccgttg
      661 cccgctggat gaagtgttcc gtcttttata cggatacaga atatgttggg tatctcctct
      721 actacataga cgcaaaaaca tcttcattgg taaaggcggt gccccaggaa gactaaaatc
      781 aaataccaaa aatcaaggat atgaaaaccc ctgcatagaa agcggatgca aaacttgcta
      841 caagagccgc tgtcaaaaca ttgggggttt tccttattct tgtattctgg gtgtatacag
      901 tcaatgtata aagtatggtt tcagtagaac ccatcattac agacgcaatc atgccggctt
      961 ttgaatccgc accgtaagtc ttgaatatgt ctgcaagaag tgccaatgag ccgctgccag
     1021 acactggtct cattatcaaa aaaggaacaa gggcgtcatc aaggccaaga aagctggtaa
     1081 cagggtgtaa aaaagaaacc agaatatcca atgctccgga tgccctgaaa acgctgattg
     1141 ctacaatcag acctacaagg gaaggaagaa gctttacaat ggtggttgct ccttcctttg
     1201 ctcccactat aaaagaatca aaacatttca cctttctgaa cactccggaa acgagaatta
     1261 aaagcaccat taccggtatt acataggaga tgaattgagt cattaccgct tcctccttcc
     1321 atcaaggata aaaaaaacaa gaagagcggt tataagggta agaactgatg taatccatat
     1381 aggcaccaaa acatctgcag gattcttcgc tccgaatgaa tttctcaagg caataatggt
     1441 tgtaggtaca agttgaaggc atgcggaatt tacaacgaga aacaaacatg tgctttttct
     1501 ggcagtggtg tttggatttg tcctttcaag ttcatggact gcagcaattc ctgatggagt
     1561 ggctccatta cccaaaccta gaaagttcgc tgtcaggttt gtgacaatat gcttttctgc
     1621 ttccttgttg taccttgttt caggaaacag tttcctaata acgggactga ttagttttga
     1681 tatgaagtta atgcctcccg catcctgaaa tatgcgcatc aagccacacc aaagggaaac
     1741 cactcccaaa agacctatgc agaaagtaac cgcatcattt gacgcatcca acaaggtttt
     1801 gctaacggca tcaatttttc cgttggcaat accaacgacc agaccaacaa tcaacaaagc
     1861 aacccagata taatttagca tattacctac ccattgggat tcctttttaa tatatatgcc
     1921 tggacaattc caaatatttg tttaaatatt aatttatcct atttctattg actttaattt
     1981 acatctaggg tatgatgata ggtgtaaata tttttgtaaa ggggaggaga ataatatgat
     2041 gaaatcaacg ggtatagtta gacgagttga cgagctaggt agagttgtac ttcccattga
     2101 gcttagaaga acactcgaca tccaagaaaa agacgctctc gagatctacg tggatgccaa
     2161 ttcaatcata ttgaagaaat atgaaccatc ctgtgtattc tgcgggaatg ccagagatgt
     2221 gattcaatac aagggaaaga atatttgccc ggattgcatt tatgacctta agaaataaca
     2281 taatatatag gcgctatcaa agggtagtgc ctttttttct ttcataaatt gcataaacat
     2341 cgtttctcga aagaccagtt tcctttgcaa cttctttaat cgcattgttt ctctttagac
     2401 cactttcaat cttttcattt accatgttga atatttcaac ctcttcgtat tctattactt
     2461 cttctacttc ccgattccct tcaataacga taacgaactc tcctcttgga ttgttttctt
     2521 catagaaccc aatcaagctg tccacactac cccatttcaa ttcttcaaac tttttcgtaa
     2581 gttctctggc aaggcaaatc ttcctgtcta ctacatgttc tttcaattcc ttaagggtgg
     2641 agagtatgtt gtgtggagcc tcataaaaca caagtgttct tttttcatca gaaatccttt
     2701 taaggacttc ccttctcttt ttcttatcct gaggtaggaa tccttcaaaa acaaaacttc
     2761 ttgtattgaa accagataaa acaacaccca ttatcaaagc gcagggaccg ggtatcagag
     2821 tatattcaat accattttca attgcgagtt tcaccaaatc ctctcccgga tcagaaatac
     2881 cgggagtacc cgcatcggta acatacgcaa tggattctcc gtttttcagc ttctctatca
     2941 acttttcacc caatgtatcc ttgttgtgct catggtaact tacaagaggt tttctaatat
     3001 caaaacgcat aagaagcttt gcggtatgcc ttgtatcttc cgctgcaatc aaatccacac
     3061 tccttaatgt atcaagtgct cttatggata tgtcctcaag attccctatt ggtgttgcaa
     3121 ttaaatacag cattttcttc tcctataaat taactttttc ctcaatcatt ttgcacgaca
     3181 gattagggtc cacaagtacg ggagtaccca ctttcaaatt ctcaccacca tacaagtttg
     3241 ccttaagcaa aaacagtatg ggactgcttg aaagctttga atgtacaaac cttatcaatt
     3301 taggttctag tttgtacttt cttaatgcaa ccaaagtgtc gcacagcctg tccggtctgt
     3361 tcaccatata caaggtgcct tttggcacca gtacctgagc t
//
LOCUS       DAONVY010000239         3400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19307_length_3400_cov_2.935426, whole genome shotgun sequence.
ACCESSION   DAONVY010000239 DAONVY010000000
VERSION     DAONVY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19307_length_3400_cov_2.935426"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLI11_06970"
     CDS             complement(<1..517)
                     /locus_tag="PLI11_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52643.1"
                     /translation="MVILKTEDLVKKFGNFTALDKVNLEVKSGEVYGFIGPNGAGKTT
                     TIRILLGILQATGGKASIFGMDSWKDAVEIHKRIAYVPGEVNLWSNLTGGEVIDLFVS
                     LRGNNSKSRRDELIEMFDLDPSKKCRTYSKGNRQKVALVAAFASDADLYILDEPTSGL
                     DPLMEKVFQKCV"
     gene            complement(529..1152)
                     /locus_tag="PLI11_06975"
     CDS             complement(529..1152)
                     /locus_tag="PLI11_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPZ52644.1"
                     /translation="MDKFLKLNPEKKKRILEAAYNEFSRNTYDNASTNRIVKEAGIGK
                     GMLFFYFNSKKDLYIYLVEEGIEYIKREYVDKIDENETDFLEKWKIISRVKFEAYKKR
                     PYIFNFMANVYLNSDMEGLPENLKRRIKEISGVGYGKLFRNIDTSLFRSDFTEEKVIR
                     YIKLMISGYEMELTERLKGTNFNEMDVQPLWDEFYEYIDDLKTIFYK"
     gene            1294..3045
                     /locus_tag="PLI11_06980"
     CDS             1294..3045
                     /locus_tag="PLI11_06980"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HPZ52645.1"
                     /translation="MFFFDGSFGTYYKQITKQDTLPEHANINNPDIVFKIHKGYVNAG
                     SNAITTNTFCANSLAFSTKEEIEDCIKRGYEIAKKAAGNNTMVFADIGYVYDNSNEIF
                     DNPQEEYLFIADTFLKLGAKNFLFETFAEYEVAKHAMKYIRQHDKDATIHITFAVSPD
                     GLTSKGLNYIDLIKSVVKDNLADGTGLNCICGPAHIKQLMAQLIQTENINLKLIAMPN
                     SGYPSRVDNMYFYDNNSSYFAKVMAEIADLGISILGGCCGTSPVHIERMIDYLTNKKA
                     SILNTNDIGKRKEKDNEEVKRSVFSQKLEKGEKPIAVELDPPLDTDIGFLVSSAQELK
                     KHGADVLTFADSPLARTRACSFLTASRIKREADVDVMPHMTCRDKNNIGISSTLIGGA
                     IDGIGNILCVTGDPASHGTKGVFSFNSYNLIEYVNRMNSEYFKSTPYFIGAALNTSAP
                     NFKNELARALKKEEMGANFFMTQPIYSKENIKNLKEAKKSLNSYILAGILPFASYKNA
                     LFLNNEVSGIEIPKEVLDSLEGKSNEEVREISISYALDITDKVYDICDGLYIMIPLKR
                     TDIVIEILEKVKSRRKI"
     gene            3042..>3400
                     /locus_tag="PLI11_06985"
     CDS             3042..>3400
                     /locus_tag="PLI11_06985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPZ52646.1"
                     /translation="MIIIGEKLNSSIPSIQTAMETRNKEEILKVIKEQAENGADFLDV
                     NTALLSNEEDAMKWIIEMIMKNTDVGIMLDSPNPKVVQACIKDIKDRKVIINSVTLVD
                     RQEYIDIALEYNAGIVAL"
BASE COUNT         1138 a          726 c          596 g          940 t
ORIGIN      
        1 ttacgcattt ttgaaacact ttctccatca atggatcaag accggatgta ggctcgtcaa
       61 gaatgtataa atccgcatcc gatgcaaaag ctgcaacaag agcaaccttc tgtctgtttc
      121 cttttgaata cgttctacac tttttggaag gatcgaggtc aaacatttca ataagttcgt
      181 ctcttctgga ttttgagttg tttcctctca aacttacaaa caaatcaatg acttcaccac
      241 cggtaagatt ggaccacaaa ttcacctcac ctggcacgta tgcaatgcgc ttatgtatct
      301 ctaccgcatc tttccatgaa tccatcccga agatgcttgc ctttccgcct gttgcctgca
      361 aaatacctaa aagaatcctt atggtggttg tcttgcctgc gccattaggt ccaataaaac
      421 cataaacctc tccgcttttt acctccaggt tcaccttgtc aagagctgtg aaatttccaa
      481 acttcttcac cagatcttcc gtttttaata taaccataaa catcctcctc atttataaaa
      541 tatggttttc aaatcatcaa tgtattcata aaactcgtcc cacaagggct gaacatccat
      601 ctcattaaaa tttgtgcctt tcagtctttc agtaagctcc atctcatatc ccgatatcat
      661 aagtttgata tacctaataa ctttttcttc cgtaaaatca cttctaaaca atgatgtatc
      721 tatattcctg aaaagcttcc catacccaac cccacttatt tcctttatcc ttcttttcaa
      781 gttctcaggc aacccttcca tatcgctgtt caaatataca tttgccatga agttaaagat
      841 atatggtctt tttttgtacg cttcaaactt caccctgctt ataatcttcc atttctcaag
      901 aaaatccgtt tcattttcat caattttgtc cacatattcc ctctttatat actcaatacc
      961 ttcttcaaca agatatatat acaaatcctt tttgctgttg aaatagaaga aaagcattcc
     1021 ctttccaata cctgcttcct tgacaatcct gttggtcgac gcattatcat acgtattcct
     1081 ggaaaactcg ttgtatgcag cttccaatat cctcttcttc ttttccggat tcagctttaa
     1141 aaacttgtcc acctattacc acctccattc atagaccgtc atggtctatg gtaacataat
     1201 agaccatggt ggtcaatagt ttttacataa aaaaccttta aaaaccaaaa ataaatggta
     1261 taatagttgg aataaaaaaa aggaggggga aaattgtttt tctttgatgg ttcctttggc
     1321 acatactata aacaaataac aaaacaggat acattgcccg agcatgcaaa tataaacaac
     1381 ccggacatcg tattcaaaat tcacaaaggg tacgtaaacg caggcagcaa cgcaataacc
     1441 accaacacct tttgtgcaaa ctcattagcc ttctcaacca aagaggaaat tgaagactgc
     1501 ataaaaagag ggtatgaaat tgctaaaaaa gctgcaggca acaacactat ggtatttgcc
     1561 gatattgggt atgtttacga caactccaac gagatctttg acaatccaca ggaggaatac
     1621 cttttcattg cagatacttt ccttaagctt ggggcaaaaa actttctgtt tgaaacgttt
     1681 gcagagtacg aagtcgcaaa gcatgcaatg aaatatatca ggcagcatga caaagacgca
     1741 acaatccata ttacctttgc agtttcaccg gacggactta caagcaaagg tctaaactat
     1801 attgatttga ttaaaagtgt agtaaaggac aatctggcag atgggacggg actaaactgc
     1861 atctgcggac ctgcccatat aaagcaattg atggctcaat taatacaaac agaaaacatt
     1921 aacttaaaac tcatagccat gcccaactca ggctacccct caagagttga caacatgtat
     1981 ttttatgata ataactcaag ctatttcgca aaagttatgg cggaaatcgc agaccttgga
     2041 atttctatac ttggaggctg ctgcggaacc agccctgttc acatagaaag aatgattgat
     2101 tatttaacaa acaagaaggc gtccatcctt aatacaaacg acataggaaa aagaaaagaa
     2161 aaagataacg aagaagtaaa aagaagtgta ttttcgcaaa agcttgaaaa gggagaaaag
     2221 cctatagcag tggaactgga tccacccctt gacacggata tcggttttct tgtttcctct
     2281 gcacaggagc ttaagaagca cggagcagac gtgctgacct tcgcagactc ccctcttgca
     2341 agaacaagag cctgcagctt ccttacagca tcaagaatca aaagggaagc ggatgttgat
     2401 gttatgcctc atatgacctg tagggataaa aacaatatag gtatatcctc aacccttata
     2461 ggtggagcaa ttgacgggat aggaaatatt ctctgtgtaa caggagatcc ggctagccac
     2521 ggtacaaagg gagtattttc cttcaactcc tacaatctta tcgaatatgt aaacaggatg
     2581 aacagtgaat acttcaaatc caccccctac ttcatagggg ccgccctgaa cacaagcgct
     2641 cccaacttca aaaacgagct tgcaagagct ttgaagaagg aagagatggg tgcaaacttc
     2701 ttcatgaccc agcccatcta ttcaaaggag aacataaaaa acttaaagga ggcaaaaaaa
     2761 tctcttaatt catacatcct tgcgggtatc ctgccttttg ccagttacaa gaatgctctg
     2821 tttttgaaca acgaggtttc aggcatagag ataccaaaag aagttttgga ttctctggaa
     2881 ggaaaatcaa acgaggaagt aagagaaatc tccatctcat atgcgcttga cataacagac
     2941 aaggtttatg acatatgtga tggtctgtat ataatgatac cacttaaaag aactgacata
     3001 gtaattgaaa tacttgaaaa agtaaaatcc aggaggaaaa tatgataatt attggagaaa
     3061 aattaaacag ttcaatccca tccattcaga cggcaatgga aacacgcaac aaagaagaga
     3121 ttttgaaagt tattaaagaa caggcggaaa acggggctga ttttcttgat gtcaatacag
     3181 ccttgttatc taatgaagaa gatgcaatga aatggataat agagatgatt atgaagaata
     3241 cggatgtcgg aatcatgctg gactcgccaa atccgaaagt tgtgcaggca tgcataaagg
     3301 acattaaaga cagaaaagtc ataatcaact ctgttacact ggtcgacagg caagaatata
     3361 ttgatattgc gcttgaatac aatgcaggaa ttgtcgccct
//
LOCUS       DAONVY010000240         3376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19465_length_3376_cov_4.336947, whole genome shotgun sequence.
ACCESSION   DAONVY010000240 DAONVY010000000
VERSION     DAONVY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19465_length_3376_cov_4.336947"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..642)
                     /locus_tag="PLI11_06990"
     CDS             complement(13..642)
                     /locus_tag="PLI11_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPZ52647.1"
                     /translation="MKILEDISLATQKGKAKDVKQLVTEALEIGVSAQEILDKGLLDG
                     MGKLGVRFKNNEVYVPEVLIAARALNNGTEILSSKLIEEGVKPIGKVVIATVKGDLHD
                     IGKNLVKMMLEGAGFEVIDLGTDVSDEEIVKVVQQEKPQILALSALLTTTMTNQKVVI
                     ETLKEANLRDKVKVMVGGAPVTQTFADEIGADGYAEDAATAAEVARMLI"
     gene            complement(661..2031)
                     /locus_tag="PLI11_06995"
     CDS             complement(661..2031)
                     /locus_tag="PLI11_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HPZ52648.1"
                     /translation="MAHVFKTIAYTSLEDFVYGKALKPVTLNNGMVIGGGVLYPEINF
                     TLPPMLITKETMPDVIRNYKEITKGICERAKELSVPGFVAEIETLPPMTENPEWGIEV
                     CKTVVDIIKEYDAKSGVKGAVRITPNDIREGNKLEHMWHGRHWENILKTFEGCAKAGA
                     DLMAIESVGGKETHDEAVMFCDISKSIFALTVLGARDMAKLWSEIVRISDKTGAIASG
                     DTACGFANTAMVLADKNYIPKVFAAAVRVISAVRSLVAFEVGAKGPHKDCGYEGVYVK
                     AITGTPISMEGKTAACAHLSHVGNIAVCVADLWSNESIQNIKLLAGMAPLVSFEQLAY
                     DCRLMNRAMETGDSILLRDLHADSDSYYDPQAYVLRPDVVLDISTELVKTKTHYERGK
                     KAALLAFEHIRSAHEKGKLKLDDRELSWLDNLSETVKTLPEDVEEFIDNVIDDCENLE
                     PKKYDM"
     gene            2243..2318
                     /locus_tag="PLI11_07000"
     tRNA            2243..2318
                     /locus_tag="PLI11_07000"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2276..2278,aa:Trp,seq:cca)
     gene            complement(2375..3292)
                     /locus_tag="PLI11_07005"
     CDS             complement(2375..3292)
                     /locus_tag="PLI11_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPZ52649.1"
                     /translation="MRKIIDFVKGIFIGIALVIPGLSGSIFAVVVGLYESILNAVNNI
                     RKEFKKSFLFLLPIVLGVGVGILASTKLVLWVTENYKQQSYFFFIGLVLGSLPLILRK
                     VKGIKFKPQYLLITVAAFVGLMLISFLLTGNVDESAAKLPKIEKITGVDDVLILTFAG
                     IFSCATMSIPGVSGSVMLMVVGHYGSVYNAVGNCADFLKALLTGNIDEAKVAASSILV
                     VLPFMIGAVIGIALIAKILVYLLKKYEAQMYYGVGGLVAGAVVTLLTEAIKDPTFTFS
                     IGVGKFLLILLIDAVLVVVGIICTKYLDK"
BASE COUNT          989 a          820 c          519 g         1048 t
ORIGIN      
        1 ccttcgccgc ttttatatca acattctagc tacttccgca gcagttgcgg catcttctgc
       61 ataaccatca gcgcctatct cgtccgcaaa ggtctgtgtt acaggagcgc caccaaccat
      121 gactttcacc ttgtcgcgca gatttgcttc ctttagtgtc tcaataacga ctttctggtt
      181 tgtcatggtc gttgtaagaa gtgctgacag agcaagtatt tggggttttt cttgctgtac
      241 aactttaact atctcttcat cacttacatc cgttcccagg tcaataactt cgaaacctgc
      301 accttccaac atcattttaa caaggttttt tccaatgtcg tgcaagtctc ccttaacagt
      361 ggcaataacg actttcccaa taggttttac accttcctca atcaactttg agcttaatat
      421 ttcggtaccg ttattcaaag ctcttgcagc aataagtact tccggtacat acacttcatt
      481 atttttgaat cgaacgccca gcttgcccat accatccaag agacccttgt caagaatctc
      541 ttgcgcagaa actccaattt caagcgcttc tgtcacgagt tgttttacat ctttagcctt
      601 tcctttttgc gttgccaatg aaatatcttc caatatcttc atttccacac tctccttaca
      661 ttacatatca tatttttttg gttccagatt ctcacaatca tctataacat tgtcaataaa
      721 ctcttctaca tcctctggaa gtgtttttac agtctcagac agattatcaa gccaggacaa
      781 ctccctgtca tcaagtttca acttaccttt ttcatgagca gaacgaatat gctcaaatgc
      841 aagcaaagct gccttctttc ctctttcata atgggtttta gtcttaacca gctccgttga
      901 aatatctaaa accacatcgg gtcttaacac atatgcttgt ggatcatagt agctgtcgct
      961 gtcagcatgt agatctctta acaaaatgct gtctccggtc tccatcgccc tgttcatcaa
     1021 cctgcagtcg tatgcaagct gctcaaagga cacaagtggc gccatgcctg caagcaattt
     1081 tatattctgt atggactcat tgctccataa atcggcaaca caaacagcaa tatttccaac
     1141 atgagacaga tgagcacaag cggcagtttt cccttccatg gatatcgggg tccctgtaat
     1201 tgcttttaca tataccccct catatccgca gtctttgtgg ggaccttttg cccctacctc
     1261 aaaggcaaca agacttctta cagcggatat tactcttaca gctgctgcaa acactttagg
     1321 aatatagttt ttgtcagcca ataccatcgc agtatttgca aaaccacagg cagtatcacc
     1381 agatgcaatc gctccagtct tatcacttat tctcactatt tcactccaga gtttcgccat
     1441 gtcccttgca cccagaaccg tcagtgcaaa tatggacttt gatatatcgc aaaacatgac
     1501 tgcttcatca tgggtttcct tcccacctac cgactcaatg gccatgaggt ctgctcccgc
     1561 ttttgcacat ccttcaaaag tttttaatat gttctcccag tgtcttccat gccacatatg
     1621 ctcaagcttg ttaccctcac gaatatcgtt tggagtaatt cttaccgctc ctttaacccc
     1681 gctttttgca tcatactcct taataatgtc aacaacagtc ttgcatactt caatacccca
     1741 ttccggattc tccgtcattg gcggcagtgt ttcaatttca gcgacgaaac caggaactga
     1801 caactcttta gctctttcac atattccttt tgttatctct ttgtaatttc tgattacatc
     1861 aggcattgtt tcttttgtaa tcagcattgg tggtagtgtg aagtttattt caggatacaa
     1921 aactcctccg ccaataacca ttccattgtt caacgttaca ggctttaacg ccttgccata
     1981 aacaaaatct tctaaacttg tatatgcgat tgttttaaat acatgagcca taatctttac
     2041 actcctttgc aattgtcttt ttatgctctt tttttataag cataaactat tagactttag
     2101 caatctatca ataattaaat attttttgaa ttaaataagg gaaaattaat gtactactac
     2161 ggacaagtct gaatgtaaaa aatttagttt atgttgttga caatactgtt ttctcatagt
     2221 ataattgtat tcgcaaaacc aaaggggagt agttcaattg gtagagcaac ggtctccaaa
     2281 accgtgtgtt gcgtgttcga gtcgcgtctc ccctgccaac aaaaaaacat ccttatgtct
     2341 gacataagga tgttttttta cccattattc ttttctactt atccaaatac ttcgtgcaaa
     2401 taatacctac aacaactagc accgcatcga ttaacaggat taaaaggaac tttcctacac
     2461 caatcgagaa cgtaaaagta gggtccttta tcgcttcagt caacagagtt acaacggctc
     2521 cagcaaccaa acctccaact ccgtaataca tttgcgcttc atacttcttt agcagataaa
     2581 caagaatttt tgcaattaaa gcaataccaa taactgctcc aatcataaat ggaagcacaa
     2641 caagaattga ggatgcagca acctttgctt catcaatatt gcctgtcagc aatgccttta
     2701 aaaagtcagc acaattccct actgcgttat aaacgctacc gtaatgccct acaaccatca
     2761 gcataacaga accactgaca cctggtatcg acatcgtagc acaggagaat atgccagcaa
     2821 acgtcagtat taaaacatca tccacaccag taatcttctc tatcttagga agttttgcag
     2881 cagactcgtc cacattaccc gtaaggagga aggaaataag cataagccca acaaaagcag
     2941 caacagttat aagaagatac tgtggcttga actttatgcc tttaactttc ctcagtataa
     3001 gtggcagact gccaagaaca agtccaataa agaaaaaata tgattgttgc ttgtagtttt
     3061 ctgttaccca taaaacaagt ttcgtagaag caagaatacc tactcccact cctaaaacta
     3121 taggaagcag aaagagaaag cttttcttga attctttacg tatattgttt acagcattca
     3181 atatggattc atatagaccg acaacaactg cgaagatgct tcctgacaag cccggaatga
     3241 ccaaggcaat accaataaag attcctttta caaaatcaat tatttttctc atgtaaaact
     3301 ccttcaaaag ttattagatt caccaacgtc aatattacta tttttttcat aattaatcaa
     3361 tagtacttga acaatc
//
LOCUS       DAONVY010000241         3370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19522_length_3370_cov_3.994872, whole genome shotgun sequence.
ACCESSION   DAONVY010000241 DAONVY010000000
VERSION     DAONVY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19522_length_3370_cov_3.994872"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1008
                     /gene="murB"
                     /locus_tag="PLI11_07010"
     CDS             70..1008
                     /gene="murB"
                     /locus_tag="PLI11_07010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004849578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPZ52650.1"
                     /translation="MNSLFKDNLFNIDDIDIMENEPMKNHTTFRIGGPARYFVTVFDT
                     GSLLEVLKACIDYDVKYMVVGNGSNLLVSCRGYDGVVIKTEGEFQNISVDDDRLTAGS
                     GVLLPRLAKAALGYSLKGLEFASGIPGSVGGGILMNAGAYDGEIRNVCAKSRYVDVNR
                     LPYLDYDRINEAVCERDNKDLLFDYRMSIFQKTGDIILDGTFQLEKVEDKSVIEEKMK
                     ELNERRRAKQPLEYQSAGSTFKRPKGNYAAKLIEECGLKGYSYGDAQVSEKHSGFVIN
                     RGNASFNDVVKVIEHVKDVVYNMTGVILEPEVKIIW"
     gene            1037..1891
                     /gene="rapZ"
                     /locus_tag="PLI11_07015"
     CDS             1037..1891
                     /gene="rapZ"
                     /locus_tag="PLI11_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPZ52651.1"
                     /translation="MKVLIVTGMSGAGRTQATKFLEDLGYFCIDNMPPMLLPKFGELY
                     LMNEMKTDKLALVMDVRSGDFFNDLITFMDDMKKANVDVSILFLDCSDEVLINRYKEN
                     RRLHPLAQSGNNLEGIRLERQRLSPLKGQSDHVIDTTNFSVWDLKNKMFELFKDDTSQ
                     TELIVHVVSFGYKYGLPIACDLLFDVRFISNPFYVPVLRHYTGNDERVKDYVLGAEET
                     KGFMKKLFDMLQFLLPLYQLEGKQALTIGIGCTGGKHRSVAIANELVKQLKVLGYRLS
                     LEHRDIGK"
     gene            1903..>3370
                     /locus_tag="PLI11_07020"
     CDS             1903..>3370
                     /locus_tag="PLI11_07020"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HPZ52652.1"
                     /translation="MEDVIYIFGHKKPDTDSICASIAYADLKRKLGFNAVACRLGEIN
                     EETRFILDYFDVPLPKYLNNVKTQVSDLEIDLVDNLSYDISIKIAWSKMQETRIKTYP
                     VVDENEKLIGIVSLSNITGKYMDAHDFNSLAACNTKIDNILETIGGVIVGGKREYYNS
                     PGKIVVAANSPDGYGDYIESGDIVITGNRVDAQKEALRLGANTLILTCGSMPCEEVQK
                     IAEEKKAVVIKTLYDSFTTARLINQSIPVGSIMTGDKGGAIVKFHIDDYVEDVRERML
                     KHRHSSYPVVDSEGHVVGLISRYHLISHKKKRVILLDHNEKSQTVKGIEEAEILEIID
                     HHKLGDLTTSRPILFINEPVGSTSTIIADLFVKNGKRPSSKIAGILCAAIVSDTLKFQ
                     SSTSTVRDYQTAQKLASIAGIDIDEFATEMFKAGTAVGVKTPEEIFNQDFKRYTLHGY
                     EIGVGQVTVYEHEKILKIKSDLIDYMNKKAENDDYALLV"
BASE COUNT         1087 a          538 c          819 g          926 t
ORIGIN      
        1 ccaggacttt tggcaatcca gacgagaaac tgggaagtgt gagaaatatt gatccatgga
       61 agtgaaaaag tgaacagttt gtttaaggat aacttgttta atatcgatga tattgatatc
      121 atggaaaatg agccaatgaa aaaccacact acttttagaa taggtggccc tgcaagatat
      181 tttgtaactg tttttgatac cggctcattg ctagaagttt taaaggcatg tattgattat
      241 gatgtcaagt atatggtagt cggtaatggc agcaatctgc ttgtttcgtg caggggttat
      301 gatggtgtgg ttataaaaac agagggagag tttcaaaaca tcagtgttga tgacgacagg
      361 ctgactgccg ggagtggcgt tttattgccg aggctggcca aggcggcgct gggatactcc
      421 ttgaagggac tggagtttgc atccgggata cccggttccg taggtggagg cattttaatg
      481 aatgccggtg catacgatgg cgagataagg aatgtatgtg caaaaagcag gtatgtggat
      541 gtaaacaggc tcccatattt ggattatgac agaataaatg aagcggtttg cgaaagggac
      601 aacaaggact tgttgttcga ttatagaatg agtatttttc agaaaactgg agacattatt
      661 ctcgatggaa cctttcagct ggaaaaggta gaagacaaaa gcgttattga agaaaaaatg
      721 aaagaactca atgaaagaag aagggcaaaa cagcctcttg agtatcaaag cgctggaagt
      781 actttcaaaa gaccaaaagg gaattacgct gcaaaactta ttgaagaatg cggtctaaag
      841 ggttacagtt atggagatgc ccaggtttct gaaaaacaca gtggttttgt aatcaatcgg
      901 ggaaatgcat cttttaatga tgtggtcaaa gttattgagc atgtaaagga tgtagtgtat
      961 aatatgacag gagttatatt ggaacccgaa gtaaagatta tatggtaaga aaatcaaaaa
     1021 gaaagtggta tagcatttga aagtattaat tgttacagga atgtccggtg cgggacgtac
     1081 acaagctaca aagtttcttg aagatctggg atacttctgc atagataaca tgcctccgat
     1141 gctgttaccg aaatttggcg agttgtattt gatgaatgag atgaaaacag acaaacttgc
     1201 ccttgtaatg gacgtaagaa gcggagactt tttcaacgat cttattactt tcatggatga
     1261 catgaaaaaa gcaaatgtgg atgtatccat tttatttttg gattgttcgg atgaggtttt
     1321 gattaataga tataaggaga accgaagact gcatcctctt gcccaatccg gaaacaatct
     1381 cgaaggaata aggttggaaa ggcagaggct gtctccttta aaggggcaat ctgaccacgt
     1441 tattgacaca accaattttt ctgtctggga tcttaagaac aagatgtttg aattgtttaa
     1501 ggatgacacc agtcagacgg aacttattgt ccatgtggtt tcttttgggt ataaatatgg
     1561 tttgccgatt gcatgtgatc ttttattcga cgtcagattc atatccaatc cgttttacgt
     1621 tcctgtgttg aggcactaca ccggaaacga tgagagggta aaggattatg tattgggtgc
     1681 tgaagagaca aaagggttta tgaaaaagct cttcgatatg ctccagtttt tacttcctct
     1741 ttaccaattg gaaggcaaac aggcattgac aattggaata ggatgcactg gaggcaaaca
     1801 cagaagtgtt gcaatagcaa atgaacttgt caaacagtta aaggttcttg ggtacagact
     1861 ttctttggag catagggata taggcaagtg acaggtgatt ttatggaaga tgttatttac
     1921 atttttggtc ataaaaaacc agatacggat tcaatttgcg catcgattgc atatgcggat
     1981 ctgaagagaa aactcgggtt caatgcagtg gcttgcagat tgggagaaat caacgaggaa
     2041 accagattta ttctcgatta ttttgatgtc ccgttgccca agtatcttaa taatgtaaag
     2101 acgcaagtaa gcgaccttga aatagacttg gtggacaatc tgtcctatga catatcgata
     2161 aagattgcat ggagtaaaat gcaggagact agaattaaga catatcccgt tgtggatgag
     2221 aatgaaaagc ttattggaat cgtctctttg tcaaatatca caggcaaata catggacgcc
     2281 catgacttca acagcctggc tgcatgcaat acaaaaatag ataatatact cgaaaccata
     2341 ggcggggtaa ttgtgggagg caaaagggag tattacaatt ctcccgggaa gattgttgta
     2401 gctgcaaatt cgcctgatgg ttatggagat tatatagaaa gtggtgatat tgttatcaca
     2461 ggtaacaggg ttgatgccca aaaggaagcc cttagacttg gagccaacac tctaatactg
     2521 acctgcggtt ccatgccctg tgaagaggta caaaagattg cagaggagaa gaaggcagta
     2581 gtaataaaga cgttgtacga ttcttttacc actgccagat tgatcaacca aagcatcccg
     2641 gttggttcaa tcatgactgg agacaagggg ggtgccattg tcaagttcca catagacgac
     2701 tatgttgaag atgtaagaga acgaatgctc aaacacaggc acagcagtta tcctgttgtt
     2761 gactcggaag gccatgtggt aggtttgatt tcaagatatc accttatttc ccataagaaa
     2821 aagcgtgtga ttcttcttga ccacaatgag aaatcacaaa ccgttaaggg aatagaggaa
     2881 gcagagattc ttgaaattat agatcaccac aaactgggcg accttacaac atcaagacca
     2941 atattgttta taaacgagcc tgtgggaagt acatctacca tcatagccga tctgtttgtg
     3001 aaaaatggaa agagaccgtc tagtaaaata gctggaattt tatgtgcggc aatagtgtcc
     3061 gacacattaa agtttcagtc ttcgacttca accgtcaggg attaccaaac agctcaaaaa
     3121 cttgcctcaa ttgcaggtat tgatattgat gagtttgcca ctgaaatgtt caaggcggga
     3181 acagctgttg gagttaaaac tcctgaagag atattcaatc aggatttcaa acgctatact
     3241 ttacatggtt acgagattgg tgtcggccag gttactgtat atgagcatga aaaaatactg
     3301 aaaataaagt cggatctgat tgattatatg aataaaaagg ctgagaatga tgactatgcg
     3361 cttttggtta
//
LOCUS       DAONVY010000242         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19759_length_3335_cov_2.971646, whole genome shotgun sequence.
ACCESSION   DAONVY010000242 DAONVY010000000
VERSION     DAONVY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19759_length_3335_cov_2.971646"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1470)
                     /locus_tag="PLI11_07025"
     CDS             complement(253..1470)
                     /locus_tag="PLI11_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPZ52653.1"
                     /translation="MNKRMYIVIFSLLVVFLASGCQAGSSKKGYIREDIDSYGKVAWK
                     EDIETRVEKQGKIYPVIDFTSVHDAIEAAIEEGGGTVYFPPGTYSIEKPIVLDLPDGV
                     KITLLGETNGHCILRGEKDIEGPVVLVLSEGVNLGNLVFQTYSQEHPAVLVKGDGTKI
                     YKCYFQGMNVRNLESTAIVAASNATVVSCTFSNVNTDAYTLEITKFPDKEAKNIYVID
                     FFLNGEFNGFVITSDDENGCPENVLIQRGVFLNYSTEQLTIKSVKNLMVDNNMLDQSA
                     MYCITMVPVYKGIDNVTIKENYIAASYRFGELKDTTAILVDGTKDSKITNVLVKNNMI
                     AYSINGMVVKGSNFTDSKVVDNVFQSTLSHGLKIEEAINLTIQRNSFKPDASSVGLQI
                     DKQNDKSIVKDNF"
     gene            complement(1557..2231)
                     /locus_tag="PLI11_07030"
     CDS             complement(1557..2231)
                     /locus_tag="PLI11_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ52654.1"
                     /translation="MKRVLSIILVIVMALGVFTACKKDGKDSPTPTPTVTPTPTPEPT
                     PTEEPTPSPTPAPGTNVALNKPFEVSSETDATFIQWGWYSEYINDGVIEHDPSAHAGW
                     TSLIKQYMTLEEFGEEWVWIDLQGVISIDKVVLWPRQDNGACFPMDYYIEVSTDNEKY
                     EKVVEVTGDDRSREGSKEPAILTFNPVDARYVRLVVTKPFDQPAGNDGYLVQLAEFEI
                     YAAPSH"
     gene            complement(2348..3031)
                     /locus_tag="PLI11_07035"
     CDS             complement(2348..3031)
                     /locus_tag="PLI11_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ52655.1"
                     /translation="MKRVTVILLVMVLALGILAACNKEGKDSPTPTPVPTDTPEPTAT
                     PTPTPEPTPEPTAEPGSNVALNKPFEVSSETDSTYVQWGWASEHINDGLIEEDPTIPH
                     VGWTSQVKQYMTLEDFEEEWVWFDLKAVYKIDKVVLWPRQDKAENFPMDYHIEVSTDD
                     KNFTKVAEVTGDTRSKDGNMDPATITFDPVDARYVRIVITKPSDLPSGSDGHLVQFAE
                     IEIFSYKEE"
BASE COUNT          843 a          721 c          673 g         1098 t
ORIGIN      
        1 cacacaaacg tgcacttgcc ttgactgcca ggaaatttgt ccggcttgtc tatgcgctgc
       61 tacgtagcaa ccggatttat acgccgccaa aaggagagta atttccaatt ggtgttccac
      121 aacttaccaa ttttgtaaat tagaattggt aagtctattg aggatggcct ttttgtctca
      181 aaaaacccta aaaaactatt aaattttcaa aaattttttc caatcacccc ttgacttttt
      241 accactggac tcctaaaaat tgtccttcac aattgatttg tcgttctgtt tgtctatctg
      301 cagaccaacg cttgaagcgt caggtttgaa ggagtttctt tgtatggtta gattgattgc
      361 ttcttctatc ttcaaaccat gacttagtgt ggactggaat acattgtcta caaccttgct
      421 gtctgtaaag ttactacctt ttacaaccat gccgtttatt gagtatgcaa tcatgttgtt
      481 ttttacaagc acattggtta ttttggaatc tttcgtgcca tcaacaagta ttgctgttgt
      541 gtctttaagt tcaccaaatc tgtaggatgc ggctatatag ttttccttta ttgtcacatt
      601 atcaatacct ttatacacag gaaccatggt tatacagtac atggcgcttt gatccagcat
      661 gttgttgtca accatgaggt ttttgacaga ctttatggtc aattgttctg tgctgtagtt
      721 gagaaaaaca cctctttgaa taagtacatt ttcaggacag ccattttcgt catcggaagt
      781 aatcacaaat ccgttgaatt ctccattcaa aaagaaatca attacgtaga tatttttggc
      841 ttccttgtct gggaatttcg ttatttcaag agtgtaggca tcagtattta cattagagaa
      901 agtacaggaa acaacagttg cattgcttgc tgcaactatt gcagtgcttt ccagattcct
      961 tacattcata ccctggaagt agcacttgta tatcttggtt ccatcccctt taacaagcac
     1021 cgccgggtgt tcctgagaat aggtctgaaa cacaagattg ccaagattta ctccctctga
     1081 caaaaccaaa accacaggac cttctatatc cttttcacct cttaaaatac agtggccgtt
     1141 ggtttcccca agaagagtta tcttgacacc atcgggcaga tccagcacaa ttggtttttc
     1201 aattgagtag gtgccgggag gaaagtagac agtgccgccg ccttcttcaa ttgccgcctc
     1261 gattgcatcg tgtacggaag taaagtcaat aacagggtat attttgccct gcttttcaac
     1321 ccgcgtctca atgtcctcct tccaggcaac tttgccatag gaatcaatat cttctctgat
     1381 gtagcctttt ttgctgctgc ctgcctggca gcctgaagcc agaaatacaa ccaaaagaga
     1441 gaatattaca atgtacattc ttttattcat ctgttttctc cttctttgtt ttctttccct
     1501 tattatatca tccaaatata aaaaatcacc acttcggaaa cgaagtggtg atttttctaa
     1561 tgactcggag ctgcatagat ttcgaactct gccagttgta ccaggtatcc gtcattgcct
     1621 gcaggttgat caaaaggttt tgtgactaca agccttacat accttgcatc aactgggtta
     1681 aaagtcagga ttgcaggttc tttgcttcct tctctggatc gatcgtctcc tgtgacttcc
     1741 acaacctttt catatttctc attgtcagtg gacacttcaa tgtagtagtc catggggaag
     1801 catgcgccat tatcctgtct tggccagaga acaaccttgt caatggatat tactccttga
     1861 agatcaatcc atacccactc ttctccaaac tcttcaagtg tcatgtactg cttgataagc
     1921 gatgtccagc ctgcatgagc actgggatcg tgttcgatca cgccatcgtt tatgtattct
     1981 gaataccaac cccattgtat aaatgttgca tccgtttctg aagaaacttc aaaaggcttg
     2041 ttaagtgcca cattggttcc gggagccggg gttggggaag gcgttggttc ttctgtagga
     2101 gtaggctctg gggtgggagt aggtgtgact gtaggtgttg gtgtaggaga gtctttgcca
     2161 tcctttttac atgccgtaaa cacaccaagc gccataacaa taaccagaat tatggataac
     2221 actcttttca tatctttgca cctccattac ggagtcaatt taattatgtc agatacataa
     2281 tagaatgact ttttccgtca aaaaaagaac ctcgtttctt cttcgaagtg gcttaattaa
     2341 tcatgtttta ttcttccttg taggagaaaa tttcaatctc agcaaactgt acaagatgtc
     2401 cgtcacttcc tgaaggcagg tcgctgggtt ttgtaatgac aatccttacg taccttgcat
     2461 ccacagggtc aaaggtaata gtggcaggat ccatgtttcc gtctttggac cttgtatctc
     2521 ccgttacttc agcaactttt gtaaaattct tgtcatcggt ggatacttca atgtggtagt
     2581 ccatcgggaa gttttccgct ttgtcctgtc ttggccagag tacaactttg tcaatcttgt
     2641 atactgcctt aaggtcgaac catacccact cttcctcaaa atcctcaagg gtcatgtact
     2701 gcttgacctg tgatgtccat cctacatgag ggatggtggg atcttcttct attagtccgt
     2761 cgttaatatg ttccgaagcc caaccccact gaacataagt ggaatccgtc tcggaagaca
     2821 cttcaaaagg cttgttgagc gctacatttg acccaggttc cgcggtaggt tcgggtgtgg
     2881 gttcgggagt gggtgttggt gtagcggttg gctccggggt gtctgtaggt actggtgttg
     2941 gagtaggtga gtctttccct tccttgttgc acgcagccag aataccaaga gccaaaacca
     3001 taacaagcaa tatgaccgtt actcttttca tgatatgtac ctccgtcgat attgtttgat
     3061 ttttatatat taaaacatat acgaggtcat gcggtcaagt gctatttgcg tcattggcgc
     3121 attgtttgat tttatatatt ataacatata cgaggtacaa aagatgttgc ttttttcaac
     3181 ttttttagtc catatatatg tgctggtatg tgtcaggttc tatcttgtac gcatccatat
     3241 gcttgtgctc aactgtaaag gagttgtccg tttttacttc aatgtaagtg ccgccaacgt
     3301 atttggtgtc atggctgtca taggtgcctg tttta
//
LOCUS       DAONVY010000243         3333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19770_length_3333_cov_3.480476, whole genome shotgun sequence.
ACCESSION   DAONVY010000243 DAONVY010000000
VERSION     DAONVY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19770_length_3333_cov_3.480476"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /gene="metG"
                     /locus_tag="PLI11_07040"
     CDS             <1..1901
                     /gene="metG"
                     /locus_tag="PLI11_07040"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPZ52656.1"
                     /translation="YYPSDNLHIGHSYTTVAADAEARYRRLRGYDVMFLTGTDEHGLK
                     IQRKAEEAGISAQEYVDRIVANIKKLWKILDVSNDKFIRTTDDFHVKAVQKIFEELYK
                     KGDIYKSEYEGWYCTPCESFWTESQLQDGNCPDCGRKVELTKEESYFFRLSKYQDRLI
                     EHIENNPDFIQPESRKNEMINNFLRPGLEDLCVSRTTFNWGIPVTFDDKHVIYVWVDA
                     LSNYITALGYNQEDDSDFRKYWPAQVHLVGKEIVRFHTIIWPAILMALDLPLPKQVFG
                     HGWLLLDGGKMSKSKGNVIDPVVLVDKYGSDAIRYFLLREVPFGSDGLFSNEALINRI
                     NSDLANDLGNLVSRTVTMQERFFGGVMPEKHEKDAIDEEIREYMLSLVPVVEGYMDKL
                     QFSNALAEIWKRVSRLNKYIDETTPWILGKDETKKDRLAGVMYTLLESIRIVSILLQP
                     FMPKTPLKIWEQIGIGKDHSWDSAKEWGLYPTGAKVSRGEILFPRIDVDKELAALEAM
                     NENKNEKKEEKKEEEPDNLITIDEFAKVEMKVALVEEASHVEGSDRLLLLKLDLGNEK
                     RQVVSGIAKYYTSEEMVGKKVVCVTNLKPAVLRGVVSEGMILAATKGKKLTLVTIDGD
                     LPAGSKIS"
     gene            1997..>3333
                     /gene="tig"
                     /locus_tag="PLI11_07045"
     CDS             1997..>3333
                     /gene="tig"
                     /locus_tag="PLI11_07045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPZ52657.1"
                     /translation="MVVKFEEISANKVKLYIESTPEQVKEKIAEAYRRNRKKYNVKGF
                     RPGKAPQKIIEQYYGKEVFFSDAFDLFVNEEYFKAIDEKKLVPYGDPKIADVVIEEDK
                     MAFSAEVSIMPMPEFGEFKGIEVPQLSDEVTDEELEAEIKQEIKRNSRLVEVTDRPAQ
                     NDDVLTVDFVGTVDGVEFEGGKAEGASVTLGEGRFIPGFEEQLVGATAGSEVTVNVTF
                     PEDYFGEDLAGKDAVFNVKVHDVKAWEEPEFNDDFVSDISEFETVDEYKQSIREKLME
                     AKRLENRKIQEDHAVRTLVETSTINLPEEMIENNIERDFNRFAENIQMYGMQIDDYAR
                     MIGKTVNEMKEEMAEESRRRLFENYALVKLAEKEELMPSPEQLDELIKLNAMEFRMTE
                     ENFRKEIQKNPGILDYLVEEGMRDSAIKLIMDNVVYVDPDKFQVEEEVEEENK"
BASE COUNT         1072 a          572 c          874 g          815 t
ORIGIN      
        1 tttactaccc aagtgacaac ctccacattg gacattcata taccacggtc gctgcagatg
       61 cagaggcaag atatagaaga ctgagaggct atgacgtaat gtttctgacc ggaactgacg
      121 agcacggtct taaaatacag agaaaagccg aggaagcagg catcagtgct caggagtatg
      181 tggacagaat tgttgcgaac ataaagaagc tgtggaagat acttgatgta tcaaacgata
      241 aattcattag aaccaccgat gattttcacg tcaaggcagt acagaaaatt tttgaggaac
      301 tgtacaaaaa aggtgacata tacaaaagcg agtatgaggg atggtactgc accccttgtg
      361 aatccttctg gacagaatcc cagttgcaag atggaaactg tcctgactgc ggaaggaaag
      421 tggagcttac caaggaagaa agctacttct tcagactgtc aaagtatcag gacagactga
      481 ttgagcatat agagaacaat cctgatttca tacagccgga atccaggaag aatgaaatga
      541 taaacaactt cctgcgtcct ggtcttgaag atttgtgcgt gtcaagaacc actttcaact
      601 ggggtatacc cgttaccttt gacgacaagc acgtcattta cgtgtgggtg gatgcccttt
      661 caaactacat taccgccctt ggatacaacc aggaagacga ttctgatttc aggaagtact
      721 ggcctgcgca ggtgcatctt gtaggaaagg aaattgttag gttccatact ataatctggc
      781 ctgctatact gatggccctt gacctgcctc ttccaaagca ggtattcggg catggctggc
      841 tgcttcttga tggaggaaaa atgtcaaaat ccaaaggcaa cgtgatagat ccggtggttt
      901 tggtcgacaa atacggttcc gatgcaataa ggtatttcct tttgagggaa gttccctttg
      961 gctcagacgg gctgttcagc aacgaagccc tcataaaccg aataaactcc gaccttgcaa
     1021 acgaccttgg aaacctggtt tcaagaacag tgacaatgca ggagagattc ttcggcggcg
     1081 taatgcccga gaaacatgaa aaggatgcca tagatgagga gattagggaa tacatgctgt
     1141 cccttgtccc ggtagttgag ggatatatgg acaagctgca gttttccaat gctcttgcag
     1201 agatatggaa gagagtctca aggctcaaca aatatattga tgagacaact ccgtggattt
     1261 tgggaaaaga tgaaaccaaa aaggacaggc tcgcaggtgt aatgtacact ttgctggaat
     1321 ccataaggat agtatccata ctgctacagc ccttcatgcc caaaacgcca ctaaagattt
     1381 gggagcagat tggcatagga aaagaccatt cctgggatag cgcaaaagaa tggggactct
     1441 atccgacagg cgcgaaggtt tcaagagggg agattctgtt tccaagaatt gatgtggaca
     1501 aggaacttgc cgctctggaa gcaatgaatg agaataagaa tgaaaagaaa gaggaaaaga
     1561 aggaagagga accagacaac ttgattacca tagacgagtt tgccaaggtg gagatgaaag
     1621 ttgctctggt cgaggaggca agtcatgtag aaggctcgga cagactgctt cttctaaaac
     1681 ttgacctggg taatgagaaa cgtcaggttg tatcaggcat agcaaaatac tatacatcgg
     1741 aagaaatggt tgggaagaag gttgtctgtg tgacgaacct gaagcctgct gttcttaggg
     1801 gagttgtgtc agaaggcatg attcttgctg ccaccaaggg caagaaactc accttggtta
     1861 ccatagacgg tgatttgcct gcaggaagca aaatttcgta gttttagttg ccggtaatat
     1921 ttatttatgg tatactttga aaatgcacct ggaacaaggg ttttgggcat gaattagagt
     1981 aggaggaata atttttatgg ttgtaaaatt tgaagagatt agtgcaaaca aggtgaaatt
     2041 atatatagaa tcaacacctg agcaggttaa ggagaaaatt gcagaagcat atagacgcaa
     2101 cagaaaaaaa tacaatgtaa aagggttcag acctggcaag gctcctcaga agattattga
     2161 gcagtactat ggcaaggaag tattttttag cgatgccttt gatctgtttg tcaatgagga
     2221 atatttcaag gctattgatg agaagaaact tgttccctat ggcgatccaa agattgccga
     2281 tgtagtaata gaagaggata aaatggcttt cagcgcagaa gtttcaatta tgccaatgcc
     2341 tgagtttgga gagttcaagg gtattgaggt tccccagttg agtgacgagg ttacagacga
     2401 ggagctggag gctgaaatca agcaggaaat caaaagaaat tcaaggcttg ttgaggttac
     2461 agacagaccg gctcaaaatg acgacgtttt gaccgttgat tttgttggta cggttgatgg
     2521 tgttgagttt gaaggcggaa aagccgaagg agcttccgtt acattgggag agggcagatt
     2581 cattcccggg tttgaagagc agctcgttgg agctactgcc ggaagcgaag taaccgttaa
     2641 tgtcacattc cctgaagact acttcggcga agatctggca ggcaaggatg cggtgtttaa
     2701 tgttaaggta catgatgtta aggcttggga agagcctgag ttcaatgacg attttgtatc
     2761 tgatatcagt gagtttgaga cagttgacga gtacaaacaa agtatcaggg aaaagcttat
     2821 ggaagcaaag cgtcttgaaa atcgcaagat tcaggaagat catgccgtaa ggacattggt
     2881 tgagacttcc acaattaacc tgcctgagga aatgatagaa aacaacatag agagagattt
     2941 taacagattt gctgaaaata tacagatgta cggcatgcaa attgatgact atgccagaat
     3001 gattggcaaa acagttaatg agatgaagga agaaatggca gaggagtccc gcaggagact
     3061 ttttgaaaac tatgcattgg ttaagcttgc cgagaaggaa gaattgatgc caagtccgga
     3121 acagctggat gagcttatca agcttaatgc aatggagttt agaatgacag aggagaattt
     3181 caggaaggaa attcaaaaga atcctggaat tttagactat ttggttgaag aaggtatgag
     3241 agattctgca atcaagctga ttatggacaa tgtggtttac gttgatcctg acaagtttca
     3301 ggttgaagaa gaggtagagg aagagaataa aga
//
LOCUS       DAONVY010000244         3322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19848_length_3322_cov_2.950107, whole genome shotgun sequence.
ACCESSION   DAONVY010000244 DAONVY010000000
VERSION     DAONVY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19848_length_3322_cov_2.950107"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLI11_07050"
     CDS             <1..747
                     /locus_tag="PLI11_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPZ52658.1"
                     /translation="VLFSIRIFAYIIAIVDVAVLSLPKEFPYAKKAKIKLKNVFALPF
                     SYKPFIYTMLITFSWTFVQNLTYSLLDYYLLNDVGVEYIFINAINAGYAIFLLVFSRF
                     WKRFLAKHSWFKTFGIAAIMYSPTTFLYSFATKATYIPIMLVVRLSQHFIGVGLNLTF
                     ANMVYVNLPEEDRTNYTSFHQFGANLSAFLGMLTGTTFVAVTKNFVINLGNFGFTSVQ
                     QLLVIQSICQLLVALMVLKLLPKVEVNNED"
     gene            complement(748..1272)
                     /gene="nrdG"
                     /locus_tag="PLI11_07055"
     CDS             complement(748..1272)
                     /gene="nrdG"
                     /locus_tag="PLI11_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPZ52659.1"
                     /translation="MTSQENRYIRIAGIIEESIVDGPGFRFVVFTQGCKRHCKGCHNE
                     ETWDLDGGKIVEIDKILKMLDENPLLDGITLSGGEPFLQPDACCEIAKGARERGLEVM
                     TYTGFTYEEISEGVDESEEVNEARRKLLQLTDILVDGPFVIEKKSLNLMFRGSSNQRL
                     LYLKDGRIIKESKD"
     gene            complement(1253..>3322)
                     /locus_tag="PLI11_07060"
     CDS             complement(1253..>3322)
                     /locus_tag="PLI11_07060"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HPZ52660.1"
                     /translation="EMNNRLMNTFADLTFKEAKENDVKRENANIDGDTAMGTMLKYGS
                     EGAKHFYELFVLKPQHSQAHKDGDIHIHDLDFLTLTTTCCQIDLITMFDNGFSTGHGH
                     LRTPNDIQSYAALACIAIQSNQNDQHGGQSIVNFDYGMADGVRKTYMKRYRANMVRSL
                     ELLLSLDNVVDTEPIMRECVESIRKETGLFPSLETNPAYFEAEQKYLTNALDKYGLFT
                     VDEIARCQRFASRKAHEETDNATYQAMEALIHNLNTMNSRAGAQTPFSSINYGLDTSP
                     EGRMVIKNVLLATEAGLGNGETAIFPIHIFRVKDGINYNPQDPSYDLFELACRVSAKR
                     LFPNFAFQDAPFNIKYYKPGNYETEVAYMGCRTRVLGNVHDPEREISARRGNLSFTSI
                     NLPRLAIEAKGDVDKFFASLDRLMNLVIDQLMERFEIQARKKVKNFPFLMGQGVWLDS
                     EKLSNEDEIREVLKHGTLSVGFIGLAETLVALIGKHHGESEEAQELGLKIIRHMRNRV
                     DAESQKRKMNFTLLATPAEGLSGRFVRMDRKRYGVIEGITDKEYYTNSFHVPVYYKIN
                     ALKKIQVEAPYHELTNAGHITYVELDGDPSKNLAAFMKIIRAMHEAGIGYGSINHPVD
                     RDPVCGYTGIINDTCPKCDRECTDDTVPVERIRRITGYLVGTLDRFNDAKRAEVRDRV
                     KHDVTRK"
BASE COUNT          824 a          820 c          633 g         1045 t
ORIGIN      
        1 gtattgtttt caatacgtat ttttgcatac ataattgcca ttgtggatgt ggctgtactt
       61 tcgcttccaa aagagtttcc ttatgcaaag aaagcaaaaa taaagctcaa gaatgtattt
      121 gcgctgcctt tttcatacaa acctttcatc tacacgatgc tgataacttt ttcctggacc
      181 tttgtccaaa accttacata ttccctgctc gactattatc tcctcaacga tgtaggagtt
      241 gaatacatat ttattaatgc gataaatgca ggctatgcca tattcctgct ggtattctca
      301 agattctgga agagatttct ggcaaaacac agctggttca aaacctttgg cattgctgcc
      361 ataatgtact ctcccaccac ctttttatac tcctttgcaa caaaagcaac atatatccca
      421 ataatgttgg tagtaaggct gtcacaacac tttataggag ttggacttaa cctcactttt
      481 gccaacatgg tgtatgttaa ccttcccgaa gaggacagga caaactacac ctcctttcat
      541 cagtttggag caaacctgtc agccttttta gggatgctaa ccggcaccac tttcgttgca
      601 gtaacaaaaa acttcgttat taacttaggt aactttggct ttaccagcgt acagcagttg
      661 cttgtgattc aatcaatctg ccagttgctg gtagctctaa tggtattaaa gcttttgcca
      721 aaggttgaag ttaataacga agattagtta atctttcgac tctttaatta ttctgccatc
      781 tttcaaatac aaaagtcttt ggttggaact gcccctgaac ataagattca ggcttttctt
      841 ttcaatcaca aacggaccgt ccaccaggat gtcggttagt tgaagaagtt tccttcttgc
      901 ttcatttacc tcttcacttt catccacacc ttctgatatt tcctcgtagg taaaccccgt
      961 ataagtcatc acctcaagac ctctttccct ggcacctttt gcgatctcgc aacaagcatc
     1021 aggctgtagg aaaggctctc cccccgacag ggttataccg tcgaggagag gattttcatc
     1081 cagcattttc agaatcttgt ctatttcaac aattttacct ccatccaaat cccaggtttc
     1141 ttcattgtgg catcccttgc agtgcctttt gcaaccttgg gtgaagacaa caaacctgaa
     1201 tccaggaccg tccacaatag actcttctat gattcctgct atgcgtatgt atctattttc
     1261 ttgtgacgtc atgtttaacc ctgtccctta cttctgcacg cttcgcatca ttaaacctgt
     1321 caagagttcc tacgagatat cctgtaattc tccttattct ctccacaggc accgtgtcgt
     1381 ccgtgcattc cctatcgcac tttggacaag tgtcgttaat tattcctgta tagccgcata
     1441 ccggatctct gtcaacagga tgattgatgc tgccataccc aatgcccgcc tcatgcattg
     1501 cacggatgat cttcataaaa gcagccaggt ttttggatgg atcaccgtcc agctccacat
     1561 acgttatatg gcctgcattt gtaagctcgt ggtatggagc ttcaacctgt atctttttaa
     1621 gtgcatttat tttgtaatat acaggaacat ggaaactgtt tgtatagtat tccttgtcgg
     1681 taattccttc aataactccg tatctctttc tgtccattct cacaaacctt ccagaaagac
     1741 cttccgcagg ggtggcaagg agcgtaaaat tcatctttct cttctggctt tccgcatcca
     1801 ctctgtttct catgtgccta ataatcttaa ggccaagttc ctgagcttct tcggattctc
     1861 cgtgatgttt tccaataaga gcaaccaaag tctcggcaag tcctatgaag cctaccgaga
     1921 gggttccgtg cttaagcact tcccttatct cgtcttcatt ggaaagcttc tcactatcaa
     1981 gccatacacc ctgacccata aggaagggga agttcttaac ctttttcctt gcctgtattt
     2041 caaacctctc cataagctga tctattacca gattcatcaa tctgtcaagg gaggcaaaga
     2101 acttgtcaac atcacccttt gcttctattg caagtcttgg caggtttatg gaggtaaagc
     2161 taagatttcc tctgcgggct gaaatctccc tttcagggtc atgcacattt ccaaggactc
     2221 ttgttctgca tcccatatat gcaacttccg tctcataatt tccaggctta taatatttga
     2281 tattgaaagg agcgtcctgg aacgcaaaat tcgggaacag cctttttgca ctgactctgc
     2341 aagccagttc aaacagatcg taacttggat cctgcgggtt gtagttgatg ccatccttca
     2401 ctctgaaaat atgaatcggg aagatggcgg tctcaccgtt gccaagaccg gcttctgttg
     2461 ccagcaatac gtttttaata accattctgc cttccggact tgtgtcaagt ccatagttga
     2521 ttgagctgaa cggggtttgc gcacctgcac ggctgttcat ggtattcaag ttgtgaatga
     2581 gagcctccat ggcctggtag gtggcattgt ccgtctcttc atgtgctttt cttgatgcga
     2641 acctctggca tcttgcaatc tcgtcaactg taaacagacc gtacttgtcc agagcatttg
     2701 taaggtattt ctgctccgcc tcaaaatatg ccggattcgt ctcaagagat gggaacagcc
     2761 ccgtttcctt tcttatactc tctacacatt cgcgcataat cggctctgta tccaccacat
     2821 tgtcaaggct gagaagaagc tccaaagacc taaccatgtt tgccctgtac ctcttcatgt
     2881 aagtcttcct gacaccgtcc gccattccat agtcaaagtt tactatggac tgtccgccat
     2941 gctggtcgtt ctgattggac tgaattgcaa tgcatgcaag cgctgcatag gactgaatat
     3001 cgtttggagt ccttaagtga ccatgtcccg tgctgaatcc attatcaaac attgtaataa
     3061 ggtctatctg acagcaggtg gtggtcaagg tcagaaaatc aaggtcgtga atatgtatgt
     3121 ccccatcctt gtgagcctgg gaatgctgag gtttaagaac aaaaagctca taaaaatgct
     3181 ttgctccttc ggaaccgtac ttcagcatgg ttcccatggc ggtgtctccg tcaatgtttg
     3241 cattctccct cttcacatca ttctcttttg cctctttgaa tgtaaggtcg gcaaaagtgt
     3301 tcatgagcct gttgttcatc tc
//
LOCUS       DAONVY010000245         3307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_19951_length_3307_cov_3.183887, whole genome shotgun sequence.
ACCESSION   DAONVY010000245 DAONVY010000000
VERSION     DAONVY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19951_length_3307_cov_3.183887"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    101..324
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            428..2080
                     /locus_tag="PLI11_07065"
     CDS             428..2080
                     /locus_tag="PLI11_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPZ52661.1"
                     /translation="MTLGDLLDRVATIYPDVEALVYHDRPYRKTYKEFNEEVDLVARG
                     LMAIGVGKGSHVAIWATNYPEWVVAMFATAKIGATLITVNTNYKIYEAEYLLRQSDAD
                     TLIMMHGLKDSCYADIINELCPTLKDSKPGELVCDELPKLKSIIYIDEKEDTPKGMFH
                     WTDLYELAKEVPHEEMKKVQETINPHDVVNMQYTSGTTGFPKGVMLTHYNILNNGKHI
                     GDNQNFTHKDRVCLPVPFFHCFGCVLGIMAIITHGATIVGVERYNPVRVMETIQAEKC
                     TATYGVPTMYIAILEHPDFNKYDYSTLRTGLMSGAQCPVRVMERVIAEMNMKEICIPY
                     GMTETSPVCTMTKMDDSIDKRVRTVGRAMPFVEVKIVDPETRKDLPPNTPGEIAVRGY
                     SVMKGYYKMPEATAQVVDENGWYYSGDIGVMDDEGYFMITGRCKDMIIRGGENIYPKE
                     IEEFLYTYPKVRDVQVIGVPDKVYGEEICACIIPKEGMEIDEAEIKEYVRENMARHKV
                     PKYVWLMDSFPMTASGKIQKYKLREKAVEALNLHDQVKVATP"
     gene            complement(2077..2826)
                     /locus_tag="PLI11_07070"
     CDS             complement(2077..2826)
                     /locus_tag="PLI11_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPZ52662.1"
                     /translation="MSDLLPQYSGVVSLWAQDVDIFNRWKLSSMLSYAQDVANSHCTL
                     LGCGWNELMADYRCCYVLTRMHLEIEKYAQSYDKVKITTWPDNKRRILFTRYFTFELE
                     SGERLGSAVSEWVLMNVDTRSIVKPADCDIKYPDTSKINAPLKMPKKIFINDDAGISF
                     SKRPVYSDFDYNGHVNNAKYIEWVCDLFPPEHYKDNPIKTIDIRYVQEIRTNDEVTLN
                     LIKKEDESAFFVKGTDSSEKSFFECYGQWQH"
     gene            complement(2875..>3307)
                     /locus_tag="PLI11_07075"
     CDS             complement(2875..>3307)
                     /locus_tag="PLI11_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPZ52663.1"
                     /translation="YPVYDLCGQLALPVVMHTGWDCYSPKVVHATPIMLREIALEFPH
                     TMFVFAHMGSLKFWDEVEDNLTGLENVYFDTSFCVNGKMDKEQARRIILKHPEENTLF
                     GSDCPWEDVDLSVSFVDSLNISDDLKERIYYLNAVNILKSG"
BASE COUNT         1039 a          642 c          727 g          899 t
ORIGIN      
        1 ctttagcatg ataacaaatc tttattgaca tttcaactct ttttatcaag gatattgaca
       61 agagatactg tattcgctat aattaactgc aaaaatattg ccaaatacgt tgaagaaaag
      121 tagtagctgt atgacgactt caagagagct tcgggttggt gggacgaagc agtaatatta
      181 cagtgaactc gtttcggagt atctaaggtg aacgcaaagt aatcttaggc gtgagcaggc
      241 gttaactgca ttaaagggag caggcagcca tggctgtttg ctaataagag tggtaccgcg
      301 gagaaatacg tcttcgtctc ttttgcaaaa gagatgaagg ctttttttat agcctttatc
      361 aggaaaaaat acgaaggagt ttggtagtgt ggaaaagatt tttgaaagag aagaccaatt
      421 gattgaaatg acattggggg atttgctgga cagggtagcc accatctatc ctgacgtgga
      481 ggcgcttgtc taccacgaca gaccctacag aaagacgtac aaagagttta atgaggaagt
      541 agatctggta gcacgtggtc ttatggccat cggtgtagga aagggcagtc atgtagccat
      601 ctgggcaacc aactacccgg aatgggttgt cgcaatgttt gcgactgcaa aaattggtgc
      661 tactttgata actgtaaata cgaactacaa gatttatgaa gcggagtact tattaagaca
      721 atcggatgct gatacgctca taatgatgca tggactcaaa gactcatgct atgcagatat
      781 aatcaacgag ctatgcccaa ctctcaagga ttcaaaacct ggcgagcttg tatgcgatga
      841 actgcctaaa ctgaagagta ttatttacat agacgaaaag gaagacactc caaaaggaat
      901 gttccattgg accgatttgt atgaacttgc aaaggaggta cctcatgagg aaatgaagaa
      961 agtgcaggaa acaataaatc ctcatgatgt tgtaaatatg cagtatacat cgggtacgac
     1021 cggatttcca aaaggagtca tgctcaccca ctacaacatc ctgaataatg gaaaacatat
     1081 aggtgacaat cagaatttta cccacaagga cagggtgtgt ctgcctgttc cgtttttcca
     1141 ctgctttggc tgcgtgctgg gaatcatggc aattattacc catggagcaa ccattgtggg
     1201 tgtggaaagg tacaaccccg ttagagtcat ggaaacaata caggcagaaa agtgtactgc
     1261 aacatacggt gtgcctacca tgtatattgc aatacttgag catcctgatt ttaacaaata
     1321 cgattacagc acattgagaa ctggccttat gtccggagca caatgccctg taagggttat
     1381 ggagagggtc attgcagaaa tgaacatgaa ggaaatctgc ataccttatg gaatgactga
     1441 aacatctcct gtgtgcacaa tgacgaaaat ggatgacagc atagacaaga gggtcaggac
     1501 tgttggtcgt gccatgcctt ttgttgaagt taaaatagtg gatcctgaga caagaaagga
     1561 tcttccccca aacacccctg gagaaattgc ggtaagagga tactcagtaa tgaaaggtta
     1621 ctacaaaatg cctgaagcca ccgcacaggt agttgatgaa aacggttggt attattctgg
     1681 tgatatcgga gttatggatg atgaaggcta cttcatgatt acaggaagat gcaaggatat
     1741 gataatccga ggcggagaaa acatttatcc gaaggaaatt gaagagtttt tatacactta
     1801 tccgaaggta agggatgtac aggtaatcgg agtgccagac aaggtatacg gcgaagagat
     1861 atgtgcctgc atcataccaa aagagggtat ggaaattgat gaggctgaga taaaggaata
     1921 tgtaagggaa aacatggcaa gacacaaagt tcccaagtac gtctggctga tggattcctt
     1981 cccaatgacc gcaagtggta agattcagaa atacaagcta agggaaaaag cagtagaagc
     2041 cttaaacctt catgatcagg tcaaggtggc aacaccttag tgctgccatt gaccgtaaca
     2101 ttcaaagaac gatttttctg atgaatccgt gccttttaca aaaaatgcgg attcatcttc
     2161 ttttttaatt aggttgagtg taacttcatc gtttgtgcga atttcctgca cataccttat
     2221 gtcaattgtc ttaatggggt tatccttgta atgctccggc gggaaaaggt cgcacaccca
     2281 ttcaatatat tttgcattgt ttacatgacc attgtagtca aaatcactgt aaacaggtct
     2341 ttttgaaaaa gaaatgcctg catcatcatt tatgaatatt ttttttggca ttttaagtgg
     2401 agcgttaatc ttgcttgtgt ccggatactt gatatcgcaa tctgcgggct tgacaatgct
     2461 tcttgtatcc acgttcataa gtacccactc ggaaacagca ctacccaagc gttctccact
     2521 ttccagttca aaggtaaagt atcttgtgaa aagaattctt cttttgttgt caggccaggt
     2581 tgttattttc accttgtcgt agctttgtgc atacttttct atttcaagat gcattcttgt
     2641 caaaacatag cagcatctgt agtctgccat tagttcattc caaccacagc ctaaaagcgt
     2701 acagtgggag tttgccacat cctgggcata actcagcatg gatgatagtt tccacctgtt
     2761 gaatatatcc acatcttgag cccataacga aacaacgcca ctgtattgag gcaataaatc
     2821 cgacattaaa ttcctccttc caaaaaatca acaacattac aattatacac aaaatcagcc
     2881 ggatttcaaa atattgactg cattaaggta ataaattctt tccttcaaat catctgatat
     2941 gtttaaagaa tcaacaaaag aaacagacaa atcaacatcc tcccaggggc agtcgctgcc
     3001 aaagagagta ttttcttcag gatgcttcag aataattctc cttgcctgtt ccttgtccat
     3061 ttttccgttt acacaaaagg atgtgtcaaa gtatacgttt tcaagtccgg tcagattatc
     3121 ttccacttcg tcccagaact ttaagcttcc catgtgggca aatacaaaca ttgtatgcgg
     3181 aaactccaaa gctatttctc taagcattat aggggttgca tggacaacct tcggactgta
     3241 gcagtcccaa ccggtatgca taacaacagg cagggccaac tggccgcata aatcatatac
     3301 tgggtac
//
LOCUS       DAONVY010000246         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20030_length_3296_cov_3.739278, whole genome shotgun sequence.
ACCESSION   DAONVY010000246 DAONVY010000000
VERSION     DAONVY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20030_length_3296_cov_3.739278"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLI11_07080"
     CDS             <1..289
                     /locus_tag="PLI11_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HPZ52664.1"
                     /translation="MSQEEYERFQKLTEEVQNLFEQAYKEGGPDSPIAQKACEKHKEW
                     LLFSWPSYSKEAHRGVAQMYVDDERFAAYYEKNIKGLATFMRDAIFIYTSK"
     gene            complement(336..959)
                     /locus_tag="PLI11_07085"
     CDS             complement(336..959)
                     /locus_tag="PLI11_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52665.1"
                     /translation="MSKKGTSWGWIIFWFACFWPVGLVLLIKKLNDKSLMMTGKTGVY
                     SAFAWFLIIIGLLAFLAFFANPLGEYEIGLLPYAVATLVGGFVILHQVRKVKKAFTRY
                     TKYIDLIQNKSVYNVDAIANTVRLPYVVVIRELQEMISEGYLNGYYINHIRRIVVAKD
                     QRPQGQGARPVNARNVVTVRCSGCGANNAVAAGVVSECEYCGSPVRG"
     gene            complement(962..1420)
                     /locus_tag="PLI11_07090"
     CDS             complement(962..1420)
                     /locus_tag="PLI11_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52666.1"
                     /translation="MVMIMNRGILLTVCIFAIVVLVVVAGFFLLDIEKVALSYWGFGS
                     LLFSLAVSMLAAITILLPKKRRNAVFYSFGLSSATTLYLTAIIVGLIASKAFEKNLNG
                     FILLQVIIHSLFIITAIIIFLASEHIRNKNVETQKKLDSGEYDRPKRGGF"
     gene            complement(1401..2624)
                     /locus_tag="PLI11_07095"
     CDS             complement(1401..2624)
                     /locus_tag="PLI11_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPZ52667.1"
                     /translation="MAVAKVVKYDGSPDIYAWKYPDTELGTWTQLIVNESQEAILFKG
                     GQALDWFGPGRHTLETANIPLLSKLVNLPFKGKSPFTAEVWFVNKVNTLDVKWGTPTP
                     IQLQDPKYNVFIPVRAHGQFGIRITDAKKFLKKLVGTLHTFTAKDMIRYFRGAYLTEV
                     KDGISEYLIMKKISALEINAYISEISESLKGSIVPTFDDYGIELVNFYVNDISVPEDD
                     SAVIKLKDALAKKAEMDIIGYSYTQERSFDTLEGAATNPGSTSASVMGAGLGLGMGVG
                     LGNNVGGAFGDIGKNINTKETKECPHCRERINTEYRFCPSCGKDTSASKNTIKCSKCG
                     TSNNVGIKFCGNCGNTLIKLCSKCGEKVEDKQRFCTNCGNVLIKKCSSCGFELNDNLR
                     YCPDCGRKVNGDDNE"
     gene            complement(2744..>3296)
                     /locus_tag="PLI11_07100"
     CDS             complement(2744..>3296)
                     /locus_tag="PLI11_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ52668.1"
                     /translation="VEAGAKIRRVEFSLNATNWSEQNEPARFVRIFADNITEHSDINV
                     FGELLPDNLAFNKKVTVSSAYDEEIWPASHIVDGLRSTRWASVDTARSNGMEWVIVDL
                     EDSYNIDAVCVYWETACPNTYIIELSDDGEVWTKITERKDTKAGLDYITVNGRGRYAR
                     ITSLDFATPYGISIFELKVFGTN"
BASE COUNT          985 a          782 c          533 g          996 t
ORIGIN      
        1 tatgtcccag gaagagtacg agaggttcca aaagcttact gaagaggttc aaaacctctt
       61 tgaacaggcg tataaagaag gtggtcccga ttcgccaatt gcccaaaaag cctgcgaaaa
      121 acacaaggaa tggcttctgt tctcatggcc aagttactca aaagaagcgc acagaggtgt
      181 tgcccagatg tatgtggatg acgaaaggtt tgctgcgtac tatgagaaga atataaaagg
      241 gttagcaacc ttcatgaggg acgccatatt catctacacc agcaaataaa acacaaatag
      301 cggactttac gtccgctatt ctgcttcctc atttattatc cacgtactgg acttccacaa
      361 tactcgcact cactaaccac gcctgctgca acagcattgt tcgcaccaca gccagaacaa
      421 cgcactgtta ctacgttcct tgcattaaca ggacgtgctc cttgcccctg tggtctttga
      481 tcttttgcta caactatacg tcttatatga tttatataat agccattaag atacccttcg
      541 cttatcattt cctgtaattc tcttattaca acaacataag ggagtcttac agtattagca
      601 atagcatcca cgttgtaaac acttttgttt tgtataagat ctatgtattt agtatatctt
      661 gtaaatgctt tcttaacttt gcgaacctga tgcaatatta caaaaccacc aacgagagtt
      721 gcaacagcat atggaagtaa acctatctca tattcaccca acggattagc aaaaaaggct
      781 aaaaaagcaa gtaagcctat tatgattaaa aaccatgcaa aagctgaata tacacctgtt
      841 ttaccagtca tcattaatga tttgtcattc agtttcttaa ttagcaaaac caatcccaca
      901 ggccaaaagc aagcaaacca gaatataatc caaccccaac ttgttccttt tttgctcatg
      961 ttcagaatcc tcccctttta ggcctgtcat attctccact atccaacttc ttctgtgttt
     1021 ccacattttt gtttcgaata tgttctgaag caagaaatat tattattgct gtaatgataa
     1081 acagcgaatg tataataacc tgaagcaaaa taaaaccatt tagatttttt tcaaaggcct
     1141 tgctggcaat caaaccgaca ataatagctg ttaaatataa cgtggttgcg gaagaaagac
     1201 caaatgagta aaaaacagca ttcctacgct tctttggtaa tagaatagta atcgccgcca
     1261 gcattgaaac tgcaagcgaa aacaacaatg acccaaaccc ccagtaacta agtgcaacct
     1321 tctcaatatc taataggaaa aaaccagcaa ctacaaccaa aacaacaata gcaaaaatac
     1381 aaacagttaa taaaataccc ctattcatta tcatcaccat tcactttcct accacaatca
     1441 ggacaatacc ttaagttgtc atttagctca aaaccacagg aagaacattt ttttatcagt
     1501 acgttaccac aattggtaca gaacctttgc ttgtcttcaa ccttttcgcc acatttacta
     1561 caaagcttta tcagcgtgtt tccgcaatta ccacagaact taattcccac gttattactg
     1621 gttccacatt ttgaacattt aatcgtgttc ttagacgcac ttgtatcttt gccacaagac
     1681 gggcagaatc tatactcagt gttgattcgc tcgcggcaat gagggcattc ctttgtttct
     1741 tttgtattta tattcttgcc aatatcacca aaggctccac caacgttgtt gccaaggcca
     1801 acgcccattc ccagacccaa tcccgctccc atcacgcttg ccgatgtcga gccaggatta
     1861 gttgcagcac cttccaatgt atcgaaagat ctctcctgtg tgtaggagta accgattata
     1921 tccatttcag ctttcttggc caatgcatcc ttcagtttta taactgctga atcgtcctca
     1981 ggaacactaa tgtcattcac ataaaagtta accagttcta taccatagtc atcaaacgta
     2041 ggtactatgc ttcccttcaa tgactcggat atttcgctaa tatatgcatt tatctccagc
     2101 gccgatattt ttttcattat aagatattcg gatatgccgt ctttaacttc agtcagataa
     2161 gcgcctctga agtatcttat catgtctttg gcagtaaagg tatgtaacgt tcccacaagt
     2221 tttttaagaa atttcttggc gtccgttatt cttattccaa attgcccatg tgccctgaca
     2281 ggtataaaaa cattgtattt gggatcttgc aactgaatag gtgtaggtgt tccccattta
     2341 acatcaagag tgtttacttt gttcacaaac catacttcgg cagtgaatgg agacttgcct
     2401 ttaaagggta gatttacaag tttactcaat agtggaatgt tcgccgtttc caaggtgtgc
     2461 cttccagggc cgaaccaatc caatgcctgt ccacctttga acaaaatggc ttcctgcgat
     2521 tcatttacga tcaactgagt ccatgtgccc agctccgtat ccggatattt ccacgcataa
     2581 atatccggcg aaccatcata cttaacaact tttgctactg ccattattgc gtcacctctg
     2641 catcatatat tctctcatct ctgttgtcag gatgatgact gtttacttta cccatactag
     2701 cataatatta acaggaacgc aatatattgt tcatgggata tcctcagttt gtgccaaata
     2761 cttttagctc aaatatcgat atcccgtatg gagttgcaaa atccagggaa gttattctcg
     2821 catagcgacc ccttccattt acagttatat aatcaagacc agctttcgta tcctttctct
     2881 ctgtaatttt tgtccacacc tctccatcat ccgacaattc aatgatataa gtattgggac
     2941 atgccgtctc ccagtaaaca caaacagcat ctatattgta actatcctca aggtcaacaa
     3001 taacccactc cattccattt gatctggcgg tgtcaacgct tgcccatctt gtactgcgta
     3061 aaccatctac aatatgtgaa gcaggccaga tttcttcatc atatgccgat gagactgtca
     3121 cctttttatt aaacgccagg ttgtcaggca ggagttctcc gaaaacatta atatctgagt
     3181 gttctgtaat gttatctgca aatattctca caaaccttgc aggttcgttt tgctcggacc
     3241 aatttgttgc atttaatgaa aattccaccc ttcgaatttt ggctcccgct tcaact
//
LOCUS       DAONVY010000247         3292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20053_length_3292_cov_3.905468, whole genome shotgun sequence.
ACCESSION   DAONVY010000247 DAONVY010000000
VERSION     DAONVY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20053_length_3292_cov_3.905468"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..2522
                     /locus_tag="PLI11_07105"
     CDS             180..2522
                     /locus_tag="PLI11_07105"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPZ52669.1"
                     /translation="MDNPNKPPYYPYVMWYNKPADNDYYKGLPIGNGNLGGKIYGTPQ
                     RDILHINECTIWTGSPYSNNLSVSKDDFDGLSRIQLRENPNQDKAWEKTFFYGMKDGN
                     KHHGQTYQYAGKVLISFPNHENHESYKRTLDLNHAVAFCSYTVQGTKYTKEYFANYPS
                     NVIASRITSSEKTMDFDITFSSDMIGDLSIEDNILIFDGITDTRKEIEGKLKFMVAVM
                     VLPRGGQIEKQGSSLKVIDAESCDILITVATNFKDYKTLGVDYKNKALSVLDLAQKKG
                     YDKLKEEHINDYTKIFKKASINLGEDNDALPIDERIEKFKENEDASLLALFFQYNRYL
                     MISSSRDGGQPANLQGIWNNLKYPPWDSKYTTNINLQMNYFPVCCANLNECANVFVDK
                     IIKLTEQGRITAEKLYGIKKGWVLHHNTDLWNITGPVDGPWGLTPTCGAWLANQLFDI
                     YLYTMDRKYLKKIYKTLKGAAEFILSFLTEYTDEEGNTYLVTCPSTSPELVQRGPEEK
                     YISFASSFDNQIVHQLFSDMIQAANTLGKDKKLIEKMQKAKEKLPSCVKIGRWGQVQE
                     FLFHDYDDPHDAHRHISNLVGVYPLSCVDLEDEEIRKAVKTTLAARSKPGDWTLWGIA
                     WRIAQYARLGDADNAYRMLKIAFDPSNRLVYPNLFGAHPIGETDKVFQIDGNFGTLAG
                     MCEIFVCSAYNQIHLLPALPDKWKEGSVYGLCARGGFLVKEIVWSDNKLVRATIYSTV
                     GGMLTVRYKDKTISVNTNTGTTYVFEYNEEEREIICSKAI"
     gene            2504..3250
                     /locus_tag="PLI11_07110"
     CDS             2504..3250
                     /locus_tag="PLI11_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPZ52670.1"
                     /translation="MLESYLNFKDYGATGDGRADDTAALNECMKEAQRTKQVCYLPSG
                     TYRVRNTLCIGSKESNKLLVIQGTGNSVIYTDDDIDMARTNTDGKVKIKEVSFRHEGK
                     SGSCLYLDDGFGHDLYGCSFSNITGNISTMLIFNGSYTDIVNCAFENAEPESYSINCT
                     TIPGKININSNIFDCRIYGTGKGLLINSSENNRPEGLKVARNMFLNTGKEQITVNSIL
                     HLDISNNMLDQSSKVSILIDPKMQGICQCH"
BASE COUNT         1120 a          624 c          707 g          841 t
ORIGIN      
        1 acttttgcaa attacattta cttcttcagt tttgcattta cttttgcaat taacctttat
       61 tcattgaatc acaacaattc ctcggaaatt gcaaataaat gaaccgagca aacccttgta
      121 acaaatactc caatatggta taactttgat actactaatg ctatggttgg tgttgaaaaa
      181 tggacaatcc aaacaaacct ccatattacc cctatgtcat gtggtacaac aaacctgccg
      241 acaacgatta ttacaaaggt ctaccaatag gaaacggaaa tttaggcgga aagatttacg
      301 gcactcctca aagggatatc ctgcatataa atgaatgcac catatggaca ggaagcccct
      361 attcaaacaa cctaagcgtt tccaaagatg actttgatgg tctaagtaga atccagctta
      421 gggaaaaccc aaaccaggac aaggcttggg aaaaaacctt cttttatgga atgaaggacg
      481 ggaacaagca ccacgggcag acgtatcaat atgctggcaa ggttctcata agttttccca
      541 accatgaaaa ccatgaaagc tacaaaagaa cgcttgattt aaatcatgcg gtcgctttct
      601 gcagttacac cgtgcaggga acgaagtata caaaagaata ctttgcaaat tacccgtcaa
      661 atgtaattgc gtcaagaata acatcatcag aaaaaactat ggactttgac attacatttt
      721 ccagcgacat gataggtgat ctttccatag aagataatat tctaatattt gatggcatca
      781 cagacacaag aaaagagatt gagggaaaac tcaagtttat ggtggcagtc atggttcttc
      841 caaggggcgg tcagattgaa aaacagggca gctctctcaa agtgattgat gcagaaagtt
      901 gcgatattct aataactgtc gccaccaatt tcaaggatta caagactctg ggtgttgact
      961 acaagaacaa ggctttatct gtattggatt tggcacagaa aaaaggatac gacaaactca
     1021 aggaagagca cataaatgac tacacaaaaa tctttaaaaa ggcatccata aatcttggcg
     1081 aagataatga tgcattacct atagatgaaa gaatagaaaa attcaaggaa aacgaggatg
     1141 catctctact tgcgctgttt ttccaataca accgctatct tatgatatct tcatcaagag
     1201 atggtggtca gccagctaat cttcaaggca tatggaacaa cctgaaatat ccaccctggg
     1261 atagtaaata tacgacgaat ataaatttgc agatgaatta ctttcctgta tgttgcgcaa
     1321 accttaatga gtgtgccaat gtgtttgttg ataaaataat caaactgaca gagcaaggca
     1381 gaatcaccgc tgaaaaactg tacggtataa aaaagggatg ggttttgcat cacaatactg
     1441 acttgtggaa cataacaggt cccgtggacg gtccttgggg ccttactcca acctgcggtg
     1501 catggctggc aaaccagctg tttgacattt atctatacac tatggacagg aaatatctca
     1561 agaaaatata caagactcta aaaggtgctg cagaattcat tctgtccttt ttaactgagt
     1621 atacggatga ggaaggaaac acttatcttg taacctgtcc ttccacgtct cctgaattgg
     1681 tacagcgagg acctgaggaa aaatacatat cttttgcctc ttcttttgac aatcaaatag
     1741 tgcaccagct gttttccgac atgatacagg cagccaatac tttaggaaaa gacaagaaac
     1801 tgatagaaaa aatgcaaaag gcaaaagaaa aactgccttc atgcgtgaaa atagggagat
     1861 ggggtcaggt ccaggagttt ttattccatg attatgatga tccgcatgat gcccatagac
     1921 atatatccaa cctggttgga gtatatcctc tgtcatgtgt tgatctggag gatgaggaga
     1981 ttagaaaagc tgttaaaaca acactggcag caagatcaaa gcctggagac tggacgttgt
     2041 ggggaattgc atggaggatt gcccaatacg caagacttgg tgacgctgat aatgcttacc
     2101 ggatgctgaa gattgccttt gatccatcca acaggcttgt ttacccaaac ctttttggag
     2161 cacatccaat aggggagacg gacaaagtgt ttcagattga cggcaacttt ggcacacttg
     2221 caggcatgtg tgaaattttt gtatgcagtg catacaacca aatacacctg ctgcctgccc
     2281 ttcctgacaa gtggaaggag ggtagtgttt atggcctatg tgcaaggggc ggattcttag
     2341 taaaagagat tgtctggtct gataacaaac tggtccgagc aaccatatac tcaaccgtgg
     2401 gtggaatgct gacagtaagg tacaaagata agacaatatc agttaatacg aatacaggaa
     2461 cgacatatgt atttgagtac aatgaagaag aaagggagat tatatgctcg aaagctattt
     2521 gaattttaag gattatggag caacaggcga tggcagggcg gatgatacag ctgccctgaa
     2581 tgaatgcatg aaggaagctc aaaggacaaa gcaggtctgc tacctgccct caggcaccta
     2641 cagagtaagg aataccttgt gtatagggag caaggaatcc aataagctgc ttgtcataca
     2701 gggaacaggc aactcggtaa tctataccga cgatgatata gacatggcaa gaaccaatac
     2761 tgacgggaaa gtcaagataa aggaagtttc ttttagacat gaaggaaaat caggaagctg
     2821 tttgtatctt gatgacggtt ttggacatga tttgtatgga tgcagttttt caaacatcac
     2881 aggcaatata agcaccatgc tcatattcaa tggatcgtat acagatattg tgaattgtgc
     2941 atttgaaaat gcagagccag aaagctattc aataaactgt actacgatac ctgggaagat
     3001 aaatattaac agcaatatat ttgactgcag aatatatggt actggaaaag gacttttgat
     3061 aaactcttca gagaacaaca ggccggaggg actcaaggta gcaagaaaca tgtttctgaa
     3121 tacaggcaag gagcagataa cggttaactc catactgcat cttgacatat caaacaacat
     3181 gttagaccag tcaagcaaag tctcaatact aatcgacccc aaaatgcagg gcatctgcca
     3241 atgtcattaa cttaagagat cgcaatacat gttttgttgc gatctcttag tt
//
LOCUS       DAONVY010000248         3282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20138_length_3282_cov_3.770685, whole genome shotgun sequence.
ACCESSION   DAONVY010000248 DAONVY010000000
VERSION     DAONVY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20138_length_3282_cov_3.770685"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PLI11_07115"
     CDS             complement(<1..1535)
                     /locus_tag="PLI11_07115"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPZ52671.1"
                     /translation="MKGIKRLLIYIVTVVILSSMLTTVSGYEYGDDIENFLYPSIEKT
                     ARATYVLNGKINKSDFNDNITKVSETSMWNGYAFLSDFSTEPYPGTQEFDSFFRNILN
                     VVNMNDSKVSIYFDFTNLAHVTNIFLEKYEEYSMEFLFLEELPLSDGETVEFKVPQGK
                     VMSIVGMNIETKALNDLTEFVQDGKLSVTAPKGSGDWKVLFFMLRKVENELLKKTCSV
                     NFLSNDASKKFIEVSYKNFVYNYPEYFPDTIDTAYMANINIANIDGGIAWAEDYNEKF
                     EEKYGFKPTLLYPALVYDIGEDYASSKTALYSLRTELLIEGFLKAMNEFASDSKIRLM
                     GNPYAFDSLVPTEYCGDFIKFFKYIDVPVFDGLSNYAVSKKYIKLVSSASYNYGKNLT
                     GANLFSPYEIIDRNTMYRTILETYSKGINHVYLSSIYNSSENMSNNDLFNKSQLAKYV
                     PGLNTLITRLNTLLQRGETVVDVGVVYPIETLNYAYTPGNNSRENIPYADYIDIGEFL
                     HQDL"
     gene            complement(1540..2817)
                     /locus_tag="PLI11_07120"
     CDS             complement(1540..2817)
                     /locus_tag="PLI11_07120"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPZ52672.1"
                     /translation="MKRPFFTYEEIKEICKKYPTPFYIYDEEGIRQGIRNLQKAFSWN
                     KNFKEYYAVKACPNPTLLRIIKEEGCGLDCSSYTELMLAEAIGCTGDDIMFSSNVTPA
                     EDYVYARELDSIINLDDFTHIEFLEEHAGLPKRICLRYNPGGEMMIGNTIMGNPGDAK
                     YGMTKEQLIQGIKILMSKGVEEFGIHAFLASNVLDNNYYVKLAEMLFELVVEINNITG
                     ANFTFVNLSGGIGIPYRPGEKCADLMYIGKGVKEAFERIIKPSNVSDDLAIKTELGRY
                     ITGPHGILVTKAIHHKDIYKNYIGVDACAANLLRPAMYGAYHHITVMGKEEEPNTHVY
                     DITGGLCENNDKFAVDRAMPEIETGDILAIHDAGAHGFAMGYNYNGKLRSAEILLKTD
                     RTTQLIRRAETPMDYFQTLDFDEDLSRIIEKRK"
     gene            complement(2904..>3282)
                     /gene="yihA"
                     /locus_tag="PLI11_07125"
     CDS             complement(2904..>3282)
                     /gene="yihA"
                     /locus_tag="PLI11_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPZ52673.1"
                     /translation="DVLLFVDLPGYGYAAVSKEERRSWGRIVEDYLNKSRNLHLIILL
                     VDIRHKPTQDDIQMMTWIRANNVNHIIAATKADKIGKTQYHKNINIIRQTLNTDCEII
                     PVSNLKKTGIDRLWEAIDKYIPL"
BASE COUNT          879 a          656 c          593 g         1154 t
ORIGIN      
        1 agatcctgat gtagaaactc tcctatgtca atatagtcgg cataagggat gttttctctt
       61 gaattgttgc ctggtgtata tgcataattc aaggtttcta taggataaac aactcctacg
      121 tcaacaacgg tttcgcctct ttgaagcagg gtattcagtc tggttatgag agtgttcagt
      181 cctggcacgt actttgccag ttgactcttg ttaaataaat cgttgttgct catgttttca
      241 cttgagttgt atattgaact cagataaaca tggttgatac ccttggagta tgtctccaga
      301 atagtcctgt acattgtatt tctatcaatt atttcatacg gactgaacaa atttgctcct
      361 gtcaagttct ttccataatt gtatgatgcg gagcttacaa gtttaatata tttttttgat
      421 actgcgtaat tagataatcc atcaaatacc ggcacatcta tgtatttgaa gaatttgata
      481 aagtctccgc agtattcagt tggcacaaga gaatcgaacg cataagggtt ccccatgagt
      541 ctgattttgc tgtcgcttgc aaactcattc attgctttaa ggaatccttc gataagtagt
      601 tcggttctta aggaatacaa agctgttttg gaagatgcat aatcttctcc tatgtcataa
      661 acaagggcgg gataaagaag tgtgggtttg aatccgtact tttcctcaaa tttctcgtta
      721 tagtcttctg cccatgcaat accgccatca atattggcta tattgatatt tgccatgtat
      781 gcagtgtcta ttgtatcagg aaaatattca ggataattgt atacaaaatt tttgtaactg
      841 acttctataa attttttgga agcatcattt gaaagaaagt tcacggagca ggttttttta
      901 agcagctcgt tttccacttt tctaagcata aaaaacagaa ccttccaatc accggaacct
      961 tttggggcag ttacagaaag ttttccgtcc tgtacaaact ctgtcaagtc attcagtgcc
     1021 tttgtttcaa tattcatgcc aactatggac atgacttttc cttgaggtac tttaaattct
     1081 actgtttcac catccgatag aggaagttct tccaaaaaca gaaactccat gctgtattcc
     1141 tcatattttt caagaaagat gtttgtgaca tgagcaagat ttgtaaagtc aaagtatatt
     1201 gatactttgg aatcattcat atttacaaca ttcagaatgt ttctgaaaaa agagtcaaac
     1261 tcctgggttc caggatacgg ctctgttgaa aaatcagata aaaaagcata tccgttccac
     1321 atactggttt cggatacttt tgtgatgttg tcattgaaat cactcttatt tatctttcca
     1381 ttcaacacat atgtagctct tgcagttttt tctatggatg ggtataaaaa gttttcaata
     1441 tcgtctccgt attcgtatcc agatacggtc gttaacatac ttgacaagat aacgaccgta
     1501 actatatata ttagaagtcg cttaatacct ttcatgctat catttccttt tttcaattat
     1561 cctgctcaag tcctcgtcaa aatcaagggt ttgaaaataa tccattggtg tttccgctct
     1621 tctaatgagc tgcgtggtcc tgtccgtttt aagaagtatt tctgccgacc tcaatttgcc
     1681 attgtagttg tagcccatgg caaacccatg tgcaccggca tcgtgtatgg caaggatgtc
     1741 acctgtctca atttctggca ttgccctgtc tactgcaaac ttgtcgttgt tttcgcataa
     1801 tccgccggta atgtcgtaga catgggtatt tggctcttct tccttaccca ttacagttat
     1861 atgatggtat gctccataca tggctggtct cagaagattt gcagcacatg cgtcaactcc
     1921 aatatagttt ttgtatatat ccttgtgatg gattgctttt gttacaagta tgccatgcgg
     1981 acctgtaatg tatctgccaa gctcagtctt tattgcaagg tcgtcactta catttgaagg
     2041 ttttataatt ctttcaaatg cctcttttac acctttccca atatacatta aatcggcaca
     2101 tttttcgcca ggtctgtaag gtatacctat tcctcctgac agattgacga atgtaaaatt
     2161 tgctcctgta atattattta tttcaacaac cagttcaaaa agcatttccg ccagttttac
     2221 ataatagttg ttgtcaagta cgttgctggc aaggaaagcg tggattccaa actcctcaac
     2281 gcccttggac ataagtattt ttatcccctg tatgagctgc tcttttgtca tcccatactt
     2341 ggcatccccg ggattgccca ttattgtgtt gcctatcatc atttctccac ccggattgta
     2401 tcttaggcaa attctttttg gcagacctgc atgttcttct aaaaactcta tatgggtaaa
     2461 atcgtcaaga ttgattatgc tgtcaagttc acgggcataa acatagtctt ctgctggagt
     2521 aacgtttgaa gaaaacatga tatcgtctcc agtgcagcca attgcttctg ccagcatgag
     2581 ttccgtgtat gacgaacagt caagtccgca tccctcttct ttaataattc taagaagtgt
     2641 gggattcgga catgctttta ctgcataata ttctttgaag tttttattcc atgaaaaggc
     2701 cttttgcaga tttctgatgc cttgtcttat tccttcttcg tcgtagatat agaatggagt
     2761 tggatatttt ttgcatattt ccttgatctc ttcataagta aaaaatggtc ttttcatcta
     2821 tcttttcatc tcctgttaca atgacttaaa acatatacag taaattatta taaattaatc
     2881 agcgtgtgtt gtaaatagag aaattagagc gggatgtact tgtcaatagc ttcccacaat
     2941 ctgtcgatgc ctgttttttt taggttcgac acaggaatga tttcgcaatc ggtatttaaa
     3001 gtctggcgga tgatgttgat gttcttatga tactgtgtct tcccaatttt atccgccttt
     3061 gttgcagcta taatgtggtt tacattgttt gcccttatcc acgtcatcat ttgaatatca
     3121 tcttgtgtag gtttgtgacg tatgtctaca agcaggatga ttagatgcag atttctgctt
     3181 ttgttcaaat aatcctctac aattcttccc cagctccttc tctcttcctt ggaaacagca
     3241 gcatatccat agccgggcag atctacaaat aacagaacat cg
//
LOCUS       DAONVY010000249         3273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20206_length_3273_cov_4.009323, whole genome shotgun sequence.
ACCESSION   DAONVY010000249 DAONVY010000000
VERSION     DAONVY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20206_length_3273_cov_4.009323"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLI11_07130"
     CDS             complement(<1..360)
                     /locus_tag="PLI11_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPZ52674.1"
                     /translation="MSLKINILKLLEQSRDKYISGEDMAFNFNVSRAAVNKAVNSLRE
                     EGHLIESVTKKGYRLLSESDVLSVEGILPYLRDDYRGNIYVFDTVESTNKTAKEIAME
                     GNEDGSVIVANGQTKGRG"
     gene            complement(437..1882)
                     /locus_tag="PLI11_07135"
     CDS             complement(437..1882)
                     /locus_tag="PLI11_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPZ52675.1"
                     /translation="MNLKMVFYVLGVIMKVEAALMMLPALVSVIYKDNELLAFLIPAV
                     LLFAIGFALSFRKPENNQLLAREGFIIVSLSWIVLSLFGSLPYVLSGEIPNFIDALFE
                     TVSGFTTTGASILTDIEALSHSTLFWRSFTNWIGGMGVLVFVLAFLPSSDTRYMYVMR
                     AEVPGPVVGKLVSKIKVTARILYVIYIVMTVLLFVFLLLGGMPVFDSIVHAIATAGTG
                     GFSIKNSSIAFYDSAYIDYVVSIFMVLFGINFNLFYFMIIGRVVTILKDEELKFYLGL
                     ILISILAVSLNIRSLYDGNILTAFRYASFQVTSIVSTSGFATADFTQWPFFSQFLIII
                     LMLTGGCAGSTAGGIKIRRFLIFIKMGFVEVKKLINPRSVVQLKVDSKVQQDTLLSGI
                     AAYFSIYVLIFAASTMIISLDAKSADTTLGSVLTCLSNVGPGFGAAGPSGNFSIFSSL
                     SKFVLMVDMLVGRLELFPIILLFTPNTWRRV"
     gene            complement(1879..3234)
                     /gene="trkA"
                     /locus_tag="PLI11_07140"
     CDS             complement(1879..3234)
                     /gene="trkA"
                     /locus_tag="PLI11_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPZ52676.1"
                     /translation="MNIVIAGIGKMGLTLVDHLVKEGHNITCIDIDPKVVDNVVNVYD
                     VFGIGGNGVSCNILKEAGIEKAQIFISTTHSDELNILNCMIAKKMGAINTIARVRNPE
                     YAMQSSFMRNELGIGLMINPELETANEISRIIRTPSAAKIETFGKGRVELAEIKIERS
                     NPLVDKTLSSLSKTFGVRVLICAVYRKDEVFIPTGDFIIREGDIVHITASHSEMSAFF
                     KAIGVYKQRAKCVIIVGGSRIAYYLAKQLVESKINVKLIENSELRSMELDEVLPKVHV
                     VCGDGTDQNLLIEEGLEEVDAFVALTGIDEENIIVSLYAKQIGIPTVISKVHKTNLLD
                     MARTLNIDSVMSPSAISANYVLQYVRAKQNAQGHNVRTLYKIVNGEVEAVEFLATDKC
                     NFLGVSFKELNLKRDLLIASIIRGNKVIIPSGNDCIEQDDIVIVVAKNLILNDLEDIL
                     P"
BASE COUNT         1020 a          766 c          511 g          976 t
ORIGIN      
        1 tcctcttccc tttgtctgac cgtttgctac aatcacactg ccatcttcat taccttccat
       61 ggcaatttcc tttgcggtct tgtttgttga ctccacagta tcaaaaacat atatattgcc
      121 tctgtagtca tctcttagat aaggaagaat accttccacc gacaaaacat cgctttcact
      181 tagcaatctg tagccctttt tggtaacaga ctctattagg tggccttctt cccttagtga
      241 gtttacagct ttatttacag cagctcttga tacgttgaaa ttaaacgcca tatcttctcc
      301 cgaaatgtat ttatccctgc tttgttccaa gagcttgagt atgtttatct tcaaagacat
      361 aaaaaaaccc ctttcgcaga atatattact cctgcaaaag ggaattgtca accatttttg
      421 ttttttgagt ttacaatcaa acccttctcc aagtattggg tgtgaacagc agaattatcg
      481 ggaacagctc aagcctgcca accagcatat ctaccataag aacaaattta gacaaagatg
      541 aaaatattga aaaattgcca gaaggccctg cagcaccaaa accaggccca acattgctca
      601 agcacgttag aaccgaacct aacgtagtat ctgcagattt ggcatccagg gatattatca
      661 ttgtgcttgc tgcaaatatt aatacataga ttgaaaagta tgccgctatc ccactcagca
      721 gggtgtcctg ctgaactttg ctatccactt tcaactgtac aacacttcta ggattaatta
      781 atttcttgac ctccacaaag cccattttta tgaaaatcag gaaacgcctt attttaatcc
      841 ctcctgcggt tgatcccgca cagcccccag tcaacattag gatgattatc aaaaactgcg
      901 agaagaacgg ccactgtgta aagtctgcgg ttgcaaaacc tgaagttgac acaatcgacg
      961 taacctgaaa ggaggcgtat cgaaacgcag taagtatgtt tccatcatat agcgatctaa
     1021 tgtttaatga tacagccaaa atcgatatca gtatcaaacc aagataaaat ttcaattcct
     1081 catcttttaa tattgtcaca accctgccaa ttatcataaa gtagaagaga ttaaagttga
     1141 taccaaaaag gaccataaaa atactgacta cataatcgat ataagcagaa tcataaaatg
     1201 cgatgctgct gtttttaata ctaaatcctc ccgttcctgc cgttgctatt gcatgcacaa
     1261 tactatcaaa aacaggcatg cctcccagca ataaaaagac aaataatagt acagtcataa
     1321 caatataaat cacataaagt atccttgccg tcacttttat ctttgaaacc aactttccaa
     1381 caacaggacc tggcacttcg gctctcatca catacatgta tcttgtatca gatgatggca
     1441 ggaatgccaa aacaaacaca agcactccca ttccaccaat ccagtttgta aaactcctcc
     1501 agaagagggt gctgtgtgat agcgcttcga tatctgtaag tatgcttgct cccgtggttg
     1561 tgaaaccgga tactgtttca aacagggcat caataaaatt tgggatttca ccacttaaaa
     1621 cataaggtag tgatccgaat aatgacaaga caatccatga taatgaaact attatgaatc
     1681 cttcccttgc cagcaactga ttgttttcag gttttctgaa agatagggca aaaccaattg
     1741 cgaatagcag aacagcggga attaaaaatg ccagcagttc attatccttg taaataacag
     1801 agaccaaggc aggcagcatc ataagtgccg cttcgacctt cataatcact ccaagtacgt
     1861 aaaaaaccat ctttaagttc atggcaaaat atcctctaga tcatttaata taaggttctt
     1921 ggcaacaaca atcacaatat catcctgctc aatgcaatca ttaccacttg gtatgattac
     1981 cttgtttcct cttattatcg aagcaataag caagtctctt tttaaattta actctttaaa
     2041 agaaacaccc agaaaattac acttgtccgt agctaaaaac tcgactgctt caacttcacc
     2101 atttactatt ttatacaagg ttcttacatt gtggccttgt gcgttctgtt ttgcacgaac
     2161 atattgaagc acatagttgg cacttattgc actggggctc atgacactat caatattcag
     2221 agttcttgcc atgtccaaca ggttcgtctt gtgaactttt gaaataacgg taggaatgcc
     2281 gatttgcttt gcatacaagg aaacaattat attctcctca tctattcccg tcaaagctac
     2341 aaaagcatcc acctcttcaa gtccttcctc gataagcagg ttttgatccg tcccatcacc
     2401 acaaacgaca tgtactttag gtaaaacctc atccagttcc atacttctta gttcactatt
     2461 ctcaatcagc ttaacattaa ttttgctctc gacaagctgc tttgccagat agtaagcaat
     2521 cctgctacct ccaactataa taacgcactt tgctctctgc ttgtaaactc caattgcttt
     2581 aaaaaaagct gacatctcgg aatgggatgc ggttatatga acaatatctc cttccctgat
     2641 tatgaagtca ccagtaggta tgaagacctc atcttttcta tacacggcac aaatcaacac
     2701 tcttacacca aaggtctttg ataacgagct tagagttttg tcaacaagag ggtttgacct
     2761 ttcaattttt atctcggcaa gttccactct gccttttcca aaggtctcta ttttcgcagc
     2821 cgagggcgtt ctgattatcc tggatatttc atttgcggtt tcaagttccg ggtttatcat
     2881 aagacctata cccaactcat ttctcataaa agagctttgc attgcatatt cagggtttct
     2941 aaccctggca atcgtattta tagcccccat tttctttgct atcatgcaat tgagaatatt
     3001 aagctcatcc gaatgcgttg ttgatatgaa tatttgggcc ttttctatac cagcctcttt
     3061 caatatattg cacgagactc cattaccacc tatgccaaac acatcataaa cgttgaccac
     3121 attatcaaca acttttggat caatatctat gcacgttata ttatgtcctt ccttaaccaa
     3181 atggtccacc aaagtaagac ccatcttgcc aattcctgca ataacaatgt tcaaaacaaa
     3241 aacccccaaa aacgtttaca tggaaattat act
//
LOCUS       DAONVY010000250         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20236_length_3268_cov_3.509181, whole genome shotgun sequence.
ACCESSION   DAONVY010000250 DAONVY010000000
VERSION     DAONVY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20236_length_3268_cov_3.509181"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLI11_07145"
     CDS             complement(<1..355)
                     /locus_tag="PLI11_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPZ52677.1"
                     /translation="MIILRQGQRGPQVEMLQLALNRAGFNAGPIDGVFGPNTLNAVLR
                     FQSSRSLTQDGIVGPATWNALRPYLVGYTTYRIRAGDTLFRISTSRNIPLQTILIANP
                     NVSPANLIVGSELILP"
     gene            complement(429..1373)
                     /locus_tag="PLI11_07150"
     CDS             complement(429..1373)
                     /locus_tag="PLI11_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPZ52678.1"
                     /translation="MKKFLFEPLRTVRRSLILDTDIGPDCDDVGALAVMYYLAKRHNT
                     KVLGVMSCSSSEYAPGCIDAINSYCGFPDIPIGTYKKPGLFEDYTKYNKFIAENFSEA
                     YKNGTLQVEDAVQLYRRLLANSPNNSVIIVTIGTLNNIAELMDSPPDAISPFSGIELI
                     QNKVYSMVSMAGKYPNGPEFNIVQHPESAKRVFENFPVPVVYSDFDLGYTVKTGFKNI
                     DEDKYKDNPIYLSYKLYTDETLENSSYDLTAVHFAFEGEGDYYNLSGPVRLTVDLEAS
                     GSNTFEEDGIEDSNQYLMRKKRTDIELSHYLNNILLSF"
     gene            complement(1417..3135)
                     /locus_tag="PLI11_07155"
     CDS             complement(1417..3135)
                     /locus_tag="PLI11_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52679.1"
                     /translation="MIKRVWNYLSRYKGMLFLTFACSLLGSILALIAPRIIGKAIDSV
                     AGEQRINLDALGKHIGTLLLLYLSSFILNRYAPLLANKVANFTARDIRKDAFEKLHNL
                     PVKYFDTHKHGDIISILSVDIENMTEGLLGLLTQMLTGIITVIGTLVFLAIMSPFVAF
                     AVLLITPLSIIIAKFITSRSSEMFKQQQNIIGELGGLAEESISGMSVIKTYLLEDTQY
                     ERFKEINARLYKAGQKAQFYSSLVNPTTRFVNHISYIAVGVSGGWLAINGVISVGQIA
                     AMLSYATQFAKPINEITGVTTQMQNAIASLRRVFTLIDAEPEKEDVSNMLEVKDGTVS
                     FEKVSFSYDSSVPLIKDLSIDVSKNNVIAIVGPTGAGKSTLVNLLMRFYDIDDGRITI
                     DDTDIFDVTRDSLRKSFAMVMQDVWIFKGTVFDNIAFGKSDATMEEVVEAAKNAHIHS
                     FIETLPKGYDTVITQEDLSVGQLQLLTIARAMLVNPPMLILDEATSSVDTRTEQYITR
                     TFNRMMQGRTSFVIAHRLSTIKDADIILVMKDGSIVEKGNHVELMKMKGMYYDLYKKS
                     LTNISN"
BASE COUNT          870 a          686 c          675 g         1037 t
ORIGIN      
        1 aggggagtat gagttcagat cctactatca ggtttgcggg actgacattg ggattagcaa
       61 tcagtattgt ttgaagggga atgtttctgc tggtggaaat tcgaaataga gtgtctccag
      121 ctcttattct ataggttgtg taacctacaa gatagggtcg tagggcgttc caagtggctg
      181 gtcctactat gccatcttgg gtaagggaac ggcttgattg aaaacgcaag actgcattca
      241 gagtgttcgg tccgaataca ccatctatgg gtcctgcgtt gaaccccgcg cggtttagtg
      301 caagttgcag catttcaact tgcggacctc tttggccttg tctgagtata atcatagaac
      361 cccctttaat tcttgaggca ttactttatt ctatgccaaa aaatcaaagg aggttctatt
      421 taggaattct agaaggaaag aagtatgttg ttcaaatagt gggatagttc aatatccgta
      481 cgctttttac gcatcagata ctggttggaa tcctcgattc catcctcttc aaaggtgttg
      541 gaaccgcttg cttccagatc gacagtcaat ctgaccggtc ctgacagatt gtagtagtct
      601 ccttcacctt cgaaggcaaa atgaactgcg gtcagatcat aacttgagtt ttcaagggtt
      661 tcatcagtgt acagcttgta cgatagataa atagggttgt ccttgtattt gtcctcatct
      721 atgttcttga atcctgtctt gaccgtgtat cccaaatcaa agtctgagta tactacagga
      781 acggggaagt tttcaaaaac cctttttgca ctttcaggat gctgtacaat attaaactca
      841 ggtccgtttg gatatttacc tgccatggat accatgctgt atactttgtt ttgaataagt
      901 tcaatgccgc taaacggact tattgcatca ggagggctgt ccatcagttc tgcgatattg
      961 ttaagtgttc ctattgtaac tataatcacg ctgttgtttg gactgttggc gagcaatctt
     1021 ctgtacagtt gaaccgcatc ttcgacttga agggtcccgt ttttatacgc ttctgagaaa
     1081 ttctctgcga tgaatttgtt atactttgtg taatcttcga acaatccggg ttttttgtat
     1141 gttccaatgg ggatatcggg aaaaccgcag taagagttga tagcatcaat acatccagga
     1201 gcgtattcgc tggatgaaca ggacattaca ccaagcactt ttgtgttatg tctcttagca
     1261 agatagtaca tgacagccaa tgctcccaca tcatcacaat ccggtccgat atcagtatca
     1321 agtatcaagc tgcgtctaac tgttcttaac ggttcgaata aaaatttctt cattgttctc
     1381 cacctttcat gaccttcgtt atattaatat tattaactag ttcgatatat ttgtcaaaga
     1441 ttttttgtac aggtcatagt acatgccttt catcttcatt aattcaacat gatttccttt
     1501 ttcaacaatg cttccgtcct tcattaccaa aattatgtcc gcatccttta tggttgaaag
     1561 ccggtgggca attacaaaag atgttctacc ttgcatcatt ctgttaaaag tgcgtgtaat
     1621 gtactgctcg gttcttgtgt cgacgctgct tgtcgcttca tctagaataa gcattggcgg
     1681 attcacaagc attgcacgtg ctatggtaag gagctgaagc tgtccaacgg acaaatcttc
     1741 ctgcgtaatc acggtgtcat aacctttagg tagtgtttca atgaaagaat gaatgtgggc
     1801 atttttagct gcttccacca cttcttccat tgtagcgtca gattttccga aggcaatgtt
     1861 atcaaagacc gtgcctttga aaatccagac atcctgcata accatggcaa aggactttct
     1921 caggctgtct cttgttacat cgaaaatgtc cgtatcgtca atggttattc ttccatcatc
     1981 tatatcgtaa aaacgcatta atagattaac cagagtgctt ttgcctgcac ctgtaggtcc
     2041 aactattgct atgacattgt tttttgagac atcaatgctt aaatccttaa tcaagggaac
     2101 actggaatca taggagaagg aaaccttctc aaaggaaacc gttccatctt ttacttcaag
     2161 catattgctt acatcctctt tttcaggctc ggcatctatc aacgtgaaca cacgcctcag
     2221 ggaagctatt gcgttctgca tttgggtggt cacccctgtt atctcattta ttggttttgc
     2281 aaactgtgtt gcatagctga gcattgcagc aatttgacca acacttatga caccgttgat
     2341 tgcaagccat ccgccagaaa ccccaacagc aatatatgag atgtggttta caaacctggt
     2401 ggttgggtta acaagggagg agtaaaactg agccttttgt cctgccttgt atagtcttgc
     2461 gtttatttct ttaaacctct catattgggt gtcttccaat aggtaagtct tgattacaga
     2521 catgccgctt atggattctt cagcaagacc tcccagttct cctataatat tctgctgctg
     2581 tttgaacatt tcagaggatc tggaggttat gaacttggca attattatgg aaagtggagt
     2641 aatcaaaagt actgcaaagg ctacaaaagg gctcattatt gctaaaaata ccaaagtccc
     2701 gatgacggta attatgcctg taagcatttg cgttaaaaga cccaatagtc cttctgtcat
     2761 attttcaatg tccactgaca aaatgctgat tatatctcca tgcttgtgcg tgtcaaagta
     2821 ttttaccggc aggttatgga gcttttcaaa agcatccttg cgaatatctc ttgccgtgaa
     2881 gtttgccacc ttgtttgcaa gcaagggagc ataacgattt agtatgaagg aggataaata
     2941 gagtaggagg agggtgccta tgtgcttacc aagcgcatcc aggtttattc tttgttctcc
     3001 ggctacagag tcgatggctt ttcctatgat ccttggtgca ataagggcaa gaatactgcc
     3061 tagaagcgag catgcaaagg taaggaaaag cattcctttg tatcttgaca agtaattcca
     3121 tactctttta atcatccata tcaccaccca gttgcgttct gcaaagctcc tgataaacct
     3181 cacattcctc catcagggtt gaatgctctc caaaaccaat gaccctgcct ttttcaagca
     3241 ccagaatgtt gttgcaattt ttgacact
//
LOCUS       DAONVY010000251         3264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20265_length_3264_cov_2.718292, whole genome shotgun sequence.
ACCESSION   DAONVY010000251 DAONVY010000000
VERSION     DAONVY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20265_length_3264_cov_2.718292"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..406
                     /locus_tag="PLI11_07160"
     CDS             203..406
                     /locus_tag="PLI11_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52680.1"
                     /translation="MFNDVKCVMTHVRGTKIAQVYTLSNISLHAYCFALGISFAPRAC
                     PTMTATAAPMENRTTFASGKQLC"
     gene            complement(650..898)
                     /locus_tag="PLI11_07165"
     CDS             complement(650..898)
                     /locus_tag="PLI11_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52681.1"
                     /translation="MKQPVSTGAVNRFKDIFKGDRKFYHAVFVLVLPIIVQNAITHFV
                     NLLDNIMIGQVGTPQMSGVAIANQLILRLPCFLLGETS"
     gene            complement(998..1690)
                     /locus_tag="PLI11_07170"
     CDS             complement(998..1690)
                     /locus_tag="PLI11_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52682.1"
                     /translation="MKKKSKKKKALIAVFVIFVVLGVVIGVFVYNMHVPKNLGVKVSK
                     EAYDSALKKLNLDKDTDKEVLDVDARLTSEEVTSLLGYNKSEDSALQKVQVRVNKDDT
                     VDFSGVVNKEFLLETMLGGSVSEESLLESAPALKYLPNKLTVYFNFSGEVVNNTTDIT
                     VNKASLMGVGLPKSLYKKKDMQESINNALSSFLKEQAEQNKGSFDMVKPQDGELVVKG
                     QLPATAFQNSGN"
     gene            1833..2582
                     /locus_tag="PLI11_07175"
     CDS             1833..2582
                     /locus_tag="PLI11_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPZ52683.1"
                     /translation="MAFKNKTVIVTGAANGIGKAVATLYAEKDACVVIADKDEENGIK
                     TAESIKKKGFNAIFLAADVRVETDIIRLMESTYKAYGHIDILINNSGLSVFKSPYELS
                     IEEWDDILNTNLRSVFLCSRKAAKYMRMKKDGGCIVNIASTRAIMSEPNSEAYAASKG
                     GIVAITHALSASFGKDRITVNAISPGWIETGDYSKLRDIDHEQHLSKRVGKPKDIARA
                     CLFLTDKENDFVTGINLVVDGGMTRKMIYEE"
     gene            complement(3065..>3264)
                     /locus_tag="PLI11_07180"
     CDS             complement(3065..>3264)
                     /locus_tag="PLI11_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPZ52684.1"
                     /translation="AFRFGSYGWSGGAEKALKEIIERNNMKWDVIDSAEFEGAPKEGH
                     LEKIEKAVLKLIEKIKDKIVE"
BASE COUNT         1017 a          699 c          558 g          990 t
ORIGIN      
        1 cataatctga aaagccccct tgaaaggctt cgaatacagc atcgtcattt acttccgtac
       61 aatttttgta tgttatcatt ctttcacctc taattcatta tactgcacca tgttctgcgc
      121 ccaaaaacct gtcctgacca ctattactcc aattatacac ttaataattt cagctgatta
      181 gcagagaggg taaagaacca gaatgtttaa tgatgtaaag tgtgtcatga cacatgtaag
      241 aggtacgaag atagcccagg tttatacact gtcaaacata agcctccatg catactgctt
      301 tgcactcggt atatcgtttg cacccagagc ctgtcctacc atgacagcaa ctgcagctcc
      361 catggagaac agaacgacat ttgcgagtgg aaaacagctg tgttaaaaag accatataaa
      421 ctcattcata agatgaggaa acctgtcaga attacagtga acatatatga caataatgga
      481 aagctctaca agccttgcca acactgttgc tattgcagct ccttcaactc ccaatgcagg
      541 taatccaagt tttcaaaaga ttaatacgta atttatgcat aacaccttcc atactccagc
      601 atggcagccc tttcagcagc atctccatca cctgtcaggt acaaagagat taactggtct
      661 ccccgagtaa gaaacaaggc aatcgcaata tcagctgatt agctattgca acacctgaca
      721 tttggggtgt gccaacctgc cctatcatga tattgtcaag taggtttaca aagtgtgtaa
      781 tggcattctg cacaattatg ggcaaaacca acacaaaaac cgcatgataa aactttctgt
      841 cccctttaaa tatatctttg aatctattaa cagcaccagt tgatacgggt tgcttcatat
      901 attttcctcc atttgctaaa agccatgcgt ttttcgcatg aggagtattt tagcataatg
      961 gaggaacata ttctagaaaa atatattggc aagattctta attaccgctg ttctgaaaag
     1021 ccgtagcagg cagctgtcct ttaacaacaa gttccccatc ctgtggttta accatatcaa
     1081 aagatccctt gttctgctca gcttgttctt tcaaaaaact gctcaaagca ttgtttatac
     1141 tttcttgcat atcctttttc ttatacaagc tttttggcaa gccaacaccc ataaggcttg
     1201 ccttgttgac agtaatatca gtcgtattgt tcacaacctc accagaaaag ttaaaataca
     1261 cggtaagctt gttaggcagg tactttaaag ctggagcaga ttcaagcaaa gattcttctg
     1321 aaactgatcc gccaagcatt gtctccaata aaaattcctt gttaacaaca ccggaaaaat
     1381 ctacagtatc atctttattc acccttacct gaaccttctg aagagcgcta tcttcagact
     1441 tgttgtatcc aagcagggat gtcacttctt cactggtaag ccttgcatcc acatccaata
     1501 cttctttgtc tgtgtctttg tccaagttca gtttttttag agcactatca tatgcctctt
     1561 tgcttacttt aactcccaga ttctttggta catgcatgtt gtatacaaat actccaataa
     1621 ctacaccaag aacaacaaat ataacaaata ctgcaataag ggcttttttc tttttacttt
     1681 ttttcttcat gtttcactcc cttaatttgg tgaaatgtgt ataaatatta taccatgtta
     1741 aaatcagatc tcaaaattat ttattgtaat tttttggtaa aaacccagga attatgatag
     1801 tattttataa actaaaaaag gaagtgatgc tgatggcttt taagaataaa acggttattg
     1861 taacgggtgc tgcaaatgga ataggtaaag ctgttgccac cctttatgct gaaaaggatg
     1921 cctgtgtggt aatagcagac aaggatgagg aaaatggaat aaagacagcg gagagtataa
     1981 agaagaaggg gtttaatgca atatttttgg cagctgatgt aagagtcgaa actgacataa
     2041 tacggctgat ggaaagcaca tataaagcat atgggcatat tgatatcctg ataaacaatt
     2101 ccgggctttc agttttcaag tctccgtatg aattatcaat tgaagaatgg gatgatattt
     2161 taaacacaaa tttaagaagt gttttcttgt gctcccgaaa agctgcaaag tacatgcgca
     2221 tgaaaaaaga tggcggctgt atagttaata ttgcatcaac aagggctatt atgtccgaac
     2281 caaactccga agcgtatgca gcttcaaaag gtgggattgt agccataacc catgctcttt
     2341 ctgcatcgtt tggaaaagac aggattacag taaacgcaat atctcctggc tggattgaga
     2401 cgggtgacta ttcaaaacta agagatattg atcacgaaca acatctgtca aaacgtgtgg
     2461 gaaagccgaa agatatcgcc agagcatgcc ttttcctgac agacaaagag aatgattttg
     2521 tcaccggtat taatttggtc gtagatggcg gcatgacccg taaaatgata tatgaggagt
     2581 aactgctact cctcatcata ctatcctgct gttctcaaac tctataaaga caagttccaa
     2641 attccggatt ttcccgaata aatgctttaa tcttcacaag gaaaatgtat tacctcttaa
     2701 acatcccaaa tactccgaac ttggggatct tggctatgta ggcattgaat gacaaatcca
     2761 actttacgta tgtatctcgg gacattttaa tttttccctt aactcttcca tcaaaaacat
     2821 tggaaattct tcttttttta catccttgcc ttcgggttca gaaatctttg ttgtcatggc
     2881 cttgtaccaa ccttttacca gagcaaaatt gccagataca acaaaccaag cttgatttta
     2941 ctgcctgatt ttctcataaa ttcctcctag tgtattaatt gccatttgca attaaacacc
     3001 tgttaaaaca aggcttcaat aaaaaacaac ccagaaaaca tccaagttgc ttttcatttt
     3061 attctcactc cacgatctta tccttaatct tttcaatgag cttcaaaact gctttttcaa
     3121 tcttttcaag atgtccttcc ttgggggctc cctcaaactc cgccgaatca atgacatccc
     3181 acttcatatt gtttctttca atgatttctt tcaacgcctt ctctgcgcca cccgaccagc
     3241 cataggagcc aaatctaaat gctg
//
LOCUS       DAONVY010000252         3263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20273_length_3263_cov_2.974751, whole genome shotgun sequence.
ACCESSION   DAONVY010000252 DAONVY010000000
VERSION     DAONVY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20273_length_3263_cov_2.974751"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1460
                     /locus_tag="PLI11_07185"
     CDS             312..1460
                     /locus_tag="PLI11_07185"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPZ52685.1"
                     /translation="MRKLNKELLNKKIEERFNQDIKNNVITCGAAIVTQNNEVLLDMK
                     KGYTIPNEEKPLQPKAIFRLASMTKPITAIAALIQVEKGKLSLDDKVSKYLPDYKDMY
                     VGRINEDRKVIKDRPAEREIEIENLLTHTSGILSDEVGVLQESVIPQKAMTDLETIVD
                     YYANHIYLSFSPMERAFYSGVGAFNVLSRIIELTSDMPYSDFLKKYIFEPLEMADTTF
                     EPTQEQWSRLVPLHTRDHEGNIIPNDNKHTFANLPVTLYSGGASLAGTIEDYKNFAEC
                     LLNEGTFKGRKIVSESLIKEMKTERLPHLFPGTGPQETWGLGVRVIKDGNILPKGTFG
                     WSGAYGTHFFIDITNNITAIYMKNSTYDGGSGAQTARNFEIDIYSCLE"
     gene            complement(1492..2025)
                     /locus_tag="PLI11_07190"
     CDS             complement(1492..2025)
                     /locus_tag="PLI11_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HPZ52686.1"
                     /translation="MEVKAATTYDYDALRALVHLSAYRKANPKTYLIAWVVSSLLFIT
                     AITFFLVFVENDIYMQAMLIVFVLIICFNLFFYFMVPKTHLKAQGSHVGIKNLYIFRD
                     DELEVKSSGKDISGCSTLKYTTLFKVMETSKYFFLFQSRAGSFIVDKSTIEDGTVDDI
                     RRVLSDTLGKKYIRCRY"
     gene            complement(2118..>3263)
                     /gene="guaA"
                     /locus_tag="PLI11_07195"
     CDS             complement(2118..>3263)
                     /gene="guaA"
                     /locus_tag="PLI11_07195"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPZ52687.1"
                     /translation="QTVNIEKVPAVFNITGKTKEGRTCSFEDEEKNIYGLLFHPEVSE
                     TVSGNEILRAFLYEACKVSGSWNMKTYVEETIEYLKEKFQDKKVLLGLSGGVDSAVCA
                     ALVGKAIGRNLTCIFVDHGFMRKNEGEQIKEVFTKQFDINLVCVDARQRFLEKLAGVT
                     DPEAKRKIIGKEFIEVFQEEARKVGAMDFFVQGTIYPDVIESGVGNIKVVKSHHNVGG
                     LPEDIQFGELVEPLRSLFKDEVRQCGLELGLPKDIVMRQPFPGPGLAIRVIGEVTEEK
                     LEIVREADHIFRDEIAKAGLDTELNQYFTVLTNMRSVGVRNNSRTYDYTIALRAVITT
                     DFMTAKWARIPYEILEKVSERIINEVEHVNRVVYDITGKPPATIEWE"
BASE COUNT         1012 a          711 c          554 g          986 t
ORIGIN      
        1 aaaaatgttc gtacaaactc atcgaatgaa gcatataaat gataaataga gctcgttaat
       61 ccctcggtaa tattagtttt aaaaatttcc tctagtaatt tttctgctgc ttgtgttata
      121 ctatacatag taaagctcac tccttgtttt attttcacaa atatattata acatattgag
      181 gggctttact ttttttgtca caaacctaga gaatctttac gctaacccca ggtgtcttct
      241 ttttcctttc atgcatttac agtcaggttt ttataggtta taattttctt aaaaaagagt
      301 aaggagaaca catgagaaag ttaaacaagg aactactaaa caaaaaaatt gaagaaagat
      361 ttaaccagga tattaagaac aacgtaatta catgtggagc tgcaatagtt acacagaaca
      421 atgaagtctt gcttgacatg aaaaagggtt acactatacc taatgaggaa aaaccccttc
      481 aaccaaaggc catattccgt ctggcttcca tgacaaaacc cataactgcg attgcagcat
      541 tgattcaagt cgaaaaagga aaactgtccc ttgatgacaa ggtatctaaa tatcttcctg
      601 attataaaga tatgtatgta ggcagaatca acgaagacag aaaagttatt aaagacagac
      661 ctgccgaaag agaaattgaa atagaaaatc ttttgacaca tacaagcggt attctatcag
      721 atgaagttgg agtactccag gaaagtgtaa taccgcaaaa agccatgacg gatttggaaa
      781 ccatagtgga ttattacgcc aatcatatat atttgtcctt ctcccctatg gaaagggctt
      841 tctacagcgg tgtcggggct ttcaacgtac tttcaagaat tattgaactt acatccgaca
      901 tgccatacag cgacttcctg aaaaaatata tttttgagcc attggaaatg gctgacacaa
      961 cctttgaacc cacacaagaa cagtggtcaa ggctggttcc actacacaca agagaccatg
     1021 aagggaacat aattccaaac gacaacaagc acacctttgc aaacttgcct gttactcttt
     1081 attcaggtgg cgcaagtctt gcaggcacaa tagaagatta caaaaacttt gcagagtgtc
     1141 ttctcaatga aggtacattc aaaggaagaa agattgtatc tgaatccctc ataaaagaaa
     1201 tgaaaaccga aagactcccc cacttgttcc ctggaacggg tccacaggaa acctggggac
     1261 ttggagttcg tgtcataaaa gacggaaata ttctgccaaa aggtactttt ggttggagtg
     1321 gcgcttatgg aacgcatttc ttcatagaca taacaaacaa cataactgcc atctatatga
     1381 aaaactccac ctatgatgga ggatcaggag ctcagacagc aagaaacttt gaaatcgata
     1441 tttactcctg tcttgaatag catgaaaggc tgccaaatca aacggcagcc tttagtatct
     1501 gcatcttatg tactttttac ctaaagtatc tgataagact cttctaatat catccaccgt
     1561 tccatcttca atagtggatt tgtcaacaat aaaagagcct gctcttgatt ggaaaagaaa
     1621 gaagtatttg cttgtctcca ttactttaaa cagcgttgta tactttaatg tcgaacagcc
     1681 gcttatatcc ttgccagatg atttaacttc cagttcatca tctctgaaaa tgtagaggtt
     1741 tttaatgcct acatgtgatc cctgagcctt taaatgagtt ttggggacca tgaaatagaa
     1801 aaacaagttg aaacatatta ttaaaacaaa cacaatcagc attgcttgca tatatatgtc
     1861 gttttctaca aatacaagaa aaaacgtaat cgcagtaata aataggagcg aagaaaccac
     1921 ccaagcaatc agatatgttt tggggtttgc cttcctgtac gcacttagat gaaccaaagc
     1981 ccttaatgca tcataatcgt atgtagtcgc tgctttaacc tccatagcac cattcccttt
     2041 caatcatgtt tttagagtta tatgcgctaa agcacttcct tcatcatttt gaaagaagtg
     2101 ctttcaaatc taaagactta ttcccattca attgttgcag ggggctttcc ggtaatatca
     2161 tagaccaccc tgttgacatg ttccacttca tttataattc gttccgacac tttctcaagt
     2221 atttcatagg gaattcttgc ccatttggca gtcatgaaat ccgttgtaat tactgctctt
     2281 aatgcgatgg tatagtcata agtcctcgaa ttgttcctga caccaacgct tctcatgttt
     2341 gtaaggactg tgaagtattg gttcaattca gtatcaagtc ctgcttttgc aatctcgtcc
     2401 ctgaagatat ggtcggcttc tctcacaatc tccagcttct cttcagttac ctcccctatc
     2461 actctgatgg caagaccagg accgggaaaa ggctgtctca ttacaatatc tttgggaagt
     2521 ccaagctcca agccgcattg tcttacctcg tccttgaaaa ggcttctcaa tggctccaca
     2581 agttctccaa actgaatatc ttctggcagt cccccgacat tgtgatggct tttaacaacc
     2641 tttatattgc ctacaccact ttcaataacg tcgggataga tggttccctg tacaaaaaag
     2701 tccattgcgc ctacctttct ggcctcttcc tgaaatactt ctataaattc cttgccaatg
     2761 atctttcttt ttgcttccgg atcagttaca cctgccaatt tttcaaggaa tctctgtctt
     2821 gcatccacac ataccagatt tatatcaaac tgcttggtaa aaacctcttt aatttgctcc
     2881 ccttcattct ttctcataaa tccatggtcg acgaatatgc aggtaaggtt cctgcctatt
     2941 gcttttccaa ccaatgctgc acatactgca gagtccactc cacctgacaa tcccaaaaga
     3001 acctttttgt cctggaattt ctccttcaag tattctatgg tctcttccac ataagttttc
     3061 atgttccagg aaccggatac tttacatgcc tcgtaaagaa acgctcttaa tatttcattg
     3121 ccgctaacgg tttccgatac ttcaggatgg aacaaaagac cataaatatt tttctcctcg
     3181 tcctcaaagg aacatgttct tccttccttt gttttgcctg taatattaaa aactgcagga
     3241 actttctcta tattaactgt ctg
//
LOCUS       DAONVY010000253         3260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20292_length_3260_cov_3.757254, whole genome shotgun sequence.
ACCESSION   DAONVY010000253 DAONVY010000000
VERSION     DAONVY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20292_length_3260_cov_3.757254"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLI11_07200"
     CDS             <1..276
                     /locus_tag="PLI11_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52688.1"
                     /translation="VGVLSDKDYREWRYDKVPYLEMVCKANLRKMSLIMKEFRNFARL
                     NKLKPSHTVYAPWGSKSRKKVLRFSKSGNPNIEKHYSTHYVKRNIEE"
     gene            complement(326..919)
                     /locus_tag="PLI11_07205"
     CDS             complement(326..919)
                     /locus_tag="PLI11_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPZ52689.1"
                     /translation="MKFLLVADEESPYIWDYFDIERYRDVDLIISAGDLKAQYLEFLV
                     SMIPVDLLYVHGNHDDGYDDNSPLGCICIENDIYIHKGIRILGLGGSYRYSNGKHQYT
                     ELEMQKRINKLKRKIKKHKGFDILVAHSPAFGHGDGKDLAHRGFECFNELIRTYKPKY
                     FIHGHQHMNYGWNQKRIYSIDETTCINAYMYHKLEYV"
     gene            complement(916..1458)
                     /locus_tag="PLI11_07210"
     CDS             complement(916..1458)
                     /locus_tag="PLI11_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52690.1"
                     /translation="MDNCLYKSVYFFFIIPVYCYVKLAPAWANSEYNTAYSSTSYGAS
                     DKYIAYTMEHDRDNSKLIYKVCGDSKDVYFQILVPTGNIAENVIVNGQEVSFENTQTH
                     NSVYANFKLSRSSNIEIDTIEVIYKVGTIKTEKETEQSNLWMYVLISITALAAIAVAA
                     LLIVKKTKKAYHDRSYETER"
     gene            1480..2856
                     /locus_tag="PLI11_07215"
     CDS             1480..2856
                     /locus_tag="PLI11_07215"
                     /inference="COORDINATES: protein motif:HMM:NF025554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HPZ52691.1"
                     /translation="MNTKDPRDLKTVELKRESIAYIVASYLGINYSADGLQLESGYST
                     DLEMHEMKRILHTIQKKSHRLINQINEKYMEYQNQPKQQMPDNVTISEMERYGYKYSK
                     AKMLPLKEKALELFDENKEVYILNSDNTEKRAIDKIELSQHLENGGLAGIRMEDFSLP
                     NEKEIITQEVLQFKAENGIEKGEFENKRFENCLFLLSSYFSKKSYDNSKFKNCIFVGS
                     NFQQSSFVNCVFENCTFDDVNFYDADLSFTEIKNCNYKDVLLEKANLNEAVIKDTEFD
                     STLVSRIKLDYTTFDNVRFKNIEEPKMVNLDKVRLIPPKNISEEAYKKHVERTLCPSP
                     NVQFEVWQLKVIPENRELLFRTYNETLEKAIILDIGNYEKVYTGKMYSDTSLENIYVT
                     LNYNPPENYKGRSMSVSDVIITTEDKGKYKAYYVDSIGFKEIPYLAEQYKMIQAQKNK
                     DLELEINN"
     gene            2871..3074
                     /locus_tag="PLI11_07220"
     CDS             2871..3074
                     /locus_tag="PLI11_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HPZ52692.1"
                     /translation="MFTVEEITLMKYCVGIEPNKEELIKNLKAQKENADNDIQDMIDE
                     VINKLTVLTDEQFRKIDFDLALF"
BASE COUNT         1182 a          507 c          622 g          949 t
ORIGIN      
        1 gttggagtac tttctgataa ggactacaga gaatggagat atgacaaggt cccatatctt
       61 gaaatggtgt gcaaggcaaa tttgcggaaa atgtctttga ttatgaaaga gtttaggaat
      121 tttgcaaggc tcaacaagct aaagccttcc catacagtat atgcgccgtg ggggtctaaa
      181 agcaggaaaa aggttttgcg tttcagcaaa tcaggaaatc caaatattga gaagcattat
      241 tcaacccatt atgtgaaaag aaatattgag gagtagaaaa agggctgctg cttttgcatc
      301 agcccgattt aatatgcatg gaacactata catattcaag tttatggtac atatatgcat
      361 taatacatgt ggtttcatct atgctgtata ttcttttttg attccagcca taattcatgt
      421 gctggtgtcc gtgaataaaa tattttggtt tgtacgtcct aatcagttca ttgaagcatt
      481 caaatccgcg atgagcaagg tcctttccat cgccatgacc aaaggcaggc gaatgcgcca
      541 ccaatatatc aaatccttta tgctttttta tttttcgttt caacttatta attctttttt
      601 gcatttctag ttccgtatac tgatgctttc catttgaata gcgatatgaa cctcccaacc
      661 ctagaatacg gattccttta tgtatataaa tatcattttc aatacagatg cagcctagag
      721 gtgaattgtc atcatatcca tcatcatggt tgccatggac gtaaaggaga tctacaggga
      781 tcatggaaac caaaaactca agatattgag ccttcaaatc gccagcggaa atgattaaat
      841 ccacatccct gtatctttca atatcaaaat agtcccaaat atatggagat tcctcatcgg
      901 cgaccagcaa aaacttcatc tttctgtctc atagctcctg tcatgatatg ctttctttgt
      961 tttcttcact atcagcagtg cagctactgc aattgctgcc aatgctgtaa ttgatattag
     1021 tacatacatc caaagattgg attgttctgt ttctttttct gtcttaatgg ttccaacctt
     1081 gtatataact tcaatggtgt caatttcaat attggaggat cttgacagtt tgaagtttgc
     1141 atacacggaa ttgtgggtct gcgtattctc aaaagatact tcctgaccgt taactattac
     1201 attttctgca atattgccag taggcaccag tatctgaaaa taaacgtctt tactatcacc
     1261 gcaaaccttg taaattagtt tgctgttgtc cctgtcgtgt tccatggtgt atgcaatgta
     1321 tttgtctgat gcaccgtagg aagttgacga gtaggcggtg ttgtattccg aatttgccca
     1381 ggcaggagca agctttacat agcaataaac aggaatgatg aagaaaaaat aaacgctctt
     1441 atacagacaa ttgtccatgc taatctttgt gaaaaggact tgaatacgaa agatccaaga
     1501 gatttaaaaa cggtagaatt gaaacgagaa agcattgctt atatagtggc cagctatttg
     1561 gggataaact atagtgctga tggactgcaa ttagaatcgg gttatagtac agatttggaa
     1621 atgcatgaaa tgaaaagaat cttacacaca atccagaaaa aaagtcatag attgataaat
     1681 caaataaacg aaaagtatat ggaatatcaa aatcaaccaa aacagcagat gcctgataat
     1741 gtaacaataa gcgaaatgga aagatatggt tataaatatt ccaaagcaaa aatgttgcct
     1801 ttaaaggaaa aggcactgga gctgtttgat gaaaacaagg aagtatacat tttaaacagt
     1861 gacaatacag aaaaaagggc aattgataag attgagcttt cacagcattt agaaaacggt
     1921 gggttggcag gtataaggat ggaagatttc agcttgccaa acgaaaaaga aataataaca
     1981 caggaagttt tgcaattcaa agcggaaaat ggaatagaaa aaggcgaatt tgaaaacaaa
     2041 aggtttgaaa attgcctgtt tttattatca agttattttt caaaaaaatc ctatgataat
     2101 tccaaattta aaaattgcat atttgtagga tcaaattttc aacaaagcag ttttgtgaat
     2161 tgtgtttttg aaaattgtac atttgatgat gtgaattttt atgatgccga tttatctttt
     2221 actgaaataa aaaactgcaa ttacaaagat gtgcttttgg aaaaagcaaa cttgaacgaa
     2281 gcagttatta aagatacaga atttgattcc acgctagtat ccagaataaa actggactat
     2341 acaacctttg acaatgtaag gttcaaaaac attgaagaac ccaaaatggt aaacttggat
     2401 aaagtgcgtc ttataccacc taaaaacatt tctgaggaag catacaagaa acatgtagaa
     2461 agaacattat gtccatctcc aaatgttcag tttgaagtat ggcaactcaa agtcatacca
     2521 gaaaacaggg aactgctttt tagaacttat aatgaaacac ttgagaaagc tataattctg
     2581 gatatcggaa attatgaaaa agtgtataca gggaagatgt atagcgatac gtcattggaa
     2641 aacatctatg taacactgaa ctataatcca ccagaaaact ataaaggaag atcaatgtct
     2701 gttagtgatg taatcataac gacagaggat aagggcaaat acaaagccta ctatgtagac
     2761 agtataggct ttaaagaaat tccgtatctt gcagaacagt ataagatgat ccaggcacaa
     2821 aaaaataagg atttggaatt ggaaataaac aactgaaagg aaggatgaaa atgtttactg
     2881 ttgaggaaat tacccttatg aagtattgtg tgggaataga accaaacaag gaagaattga
     2941 taaagaattt aaaggcacaa aaggaaaatg ccgataatga tatacaggac atgattgatg
     3001 aggtaattaa caaactaact gtattgaccg atgaacagtt tagaaaaata gactttgatt
     3061 tggctttgtt ttaatgaata ttgaaaagaa taaatagaaa attaagaaaa aataaataaa
     3121 aaccaccgct tgacatataa taaatatgta gtataatatt aaatggttat aataataacc
     3181 ttaatgatta acaaaacttt ttgttagaaa gagggagaac ccctgccaga ccttgagttg
     3241 gtcgctaagt ggggaacgca
//
LOCUS       DAONVY010000254         3254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20348_length_3254_cov_3.238825, whole genome shotgun sequence.
ACCESSION   DAONVY010000254 DAONVY010000000
VERSION     DAONVY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20348_length_3254_cov_3.238825"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1734)
                     /locus_tag="PLI11_07225"
     CDS             complement(70..1734)
                     /locus_tag="PLI11_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52693.1"
                     /translation="MKYGPVAGYDRLGRKLPMIEDVGPKRKDKYVGIFYFVHRSSSSE
                     KRMVVNVDKLLKETPEAALDYDHPAWGTGRCVYHWGEPLFGYYAADDEWVIRKHVEML
                     SYADIDFIVFDTTNRLTFKHNCMTVIRIIDEYIQRGFKVPKVVYYTNTKSGETVNEIY
                     EDVYKARFCPDAWFYYEGKPLIIGNPEECSEEAREFFTFRLPQWPTEPFKQGGFPWMS
                     FERPQYVFRDKNGNPEVISVSVAQHPQIKFGDSAMYGEVRNRGRAFHDGYNDKTKGAL
                     NWGFNIAEQWEYAIAQDPKIIFVTGWNEWSMGRIRGSKERPVTFIDQANQEFSRDIEP
                     MRDGHFDDYYMQLIDYVRRFKGMDEIKPGMEKTIDFNGPFSQWEDVEPTFYDFPFGSC
                     HRDHFGVDGERYVNTTGRNDIDIMKICHDKDNIYFYVSTYENIKGYSFTPWMRLFLHI
                     AGNEFIGWERYQYALNLELVDGNNSLIHKSLGAWRFVPVGRAPLRYAGKELMLKVPRK
                     LVGLDKTPFEIHFKWADGIAGDWSIEDFYLNGDTAPYGRLNYVYRS"
     gene            complement(1744..>3254)
                     /locus_tag="PLI11_07230"
     CDS             complement(1744..>3254)
                     /locus_tag="PLI11_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52694.1"
                     /translation="DYFRWNEFLTGGGTDHPEGGFIDRNYYSQAFACSLAVLNKTPIA
                     YNGSWGSPHSVLRHLRNVGAAYGNLTGRFEHNLVQAFSPRLTNVLALYPLDLNYVEER
                     FGSWMVQFGYCNYITEEKIHQHFEKVEDGYIFISGRKYSTLLVLFSPFIKKETLELMK
                     KFVDSGGKVVWTSMHPVDTLSSPLVRGDFEELFGVSACEKAYLERTFKDRKIRFENSL
                     NGVLDMEILTDLRPDFVYPVTPKEGTEVAARLDEFIVGTVKRYPSGGLCVYFGARLRD
                     DQSTSTGRDVDTLFSVLEKLGCYDGSELEVMSRNKDSRYLISRFPNGSVSVTNHYRTF
                     YEEWDGKFFRDEKEDEKALEGRILPPVELNLEKFSILGSEISYNGIDLLTYRLDENGN
                     LVGFAGSATTGIVIGNAEYKFCGEKADIAFGVVDLHLEDNVEKLFVVKTNKKQRIKVP
                     FPEDANGFKAYVCGELVFSADREIGFEFLDDSIEIDIDDFAVNKWIAVVKMK"
BASE COUNT          891 a          846 c          548 g          969 t
ORIGIN      
        1 aaccgaataa gttagggttc tttaaaacga gtttcttaag ttaatgacat tgaggcaatg
       61 cctgcttttt tagcttctgt acacataatt caaccttcca tagggagccg tgtcaccgtt
      121 caaataaaag tcttcaattg accagtcacc tgctatgcca tctgcccatt tgaagtgaat
      181 ttcaaaggga gtcttgtcaa gcccaacaag ttttcttggc actttcagca taagctcctt
      241 tcctgcatat ctaagaggtg ctcttcccac aggaacaaaa cgccatgcgc caagactctt
      301 gtgtattagc gagttgttac cgtcaaccag ctccagattc aaagcgtact ggtatctctc
      361 ccaccctata aactcatttc ccgctatatg cagaaacaat ctcatccatg gagtaaaaga
      421 atacccctta atattctcat acgtactcac gtaaaagtag atattgtcct tgtcatggca
      481 tatcttcatt atgtctatat catttctgcc tgttgtgttt acatatcttt ctccatcaac
      541 accaaaatga tccctgtggc agctgccaaa cgggaaatca taaaaggttg gctccacatc
      601 ctcccactgg gaaaatggtc cgttaaaatc aatagttttc tccattcccg gctttatttc
      661 atccataccc ttgaatcggc gcacgtagtc aataagctgc atgtagtagt catcaaagtg
      721 tccatctctc ataggctcaa tatctcttga gaactcctgg tttgcctggt caataaaagt
      781 tacaggacgt tctttggatc cccttatcct tcccatggac cattcattcc atccggtaac
      841 aaatattatc ttcgggtctt gtgcaatggc atactcccac tgctcggcaa tattgaagcc
      901 ccagttcaat gcaccctttg tcttgtcgtt atacccatca tgaaaagccc tgcctctgtt
      961 tcttacctct ccatacatgg cagaatcccc aaacttgatc tgcggatgct gggcaacaga
     1021 cacactaata acttcagggt tgccattctt gtccctgaat acatactgcg gcctttcaaa
     1081 gctcatccag ggaaacccgc cctgcttgaa aggctccgta ggccattggg gcagcctaaa
     1141 ggtaaaaaac tctcttgcct cttcggaaca ctcttcagga ttgccaataa ttaatggttt
     1201 tccttcatag tagaaccaag catccgggca aaaccttgcc ttgtacacat cctcatatat
     1261 ctcgttcact gtctctccgg atttcgtatt tgtgtaatat acaactttgg gtactttgaa
     1321 tcctctttga atgtattcgt caattattct aataaccgtc atgcagttgt gcttgaaggt
     1381 tagtctgttt gtggtgtcaa acactatgaa atcaatatcc gcatacgaaa gcatctccac
     1441 atgcttccgt ataacccatt catcatcagc agcatagtag ccaaagagcg gctcacccca
     1501 gtggtagaca catctgcccg tcccccaggc tggatggtcg taatccagtg ctgcttccgg
     1561 ggtctctttc agcagtttgt ccacattcac aaccatcctt ttttcacttg aagaacttct
     1621 gtgaacaaaa taaaagattc caacatactt gtcttttcgt tttgggccca catcctcaat
     1681 catcgggagt tttcttccca atctgtcata tcctgcaaca ggcccatatt tcataaaaac
     1741 acctcatttc attttaacca ctgctatcca cttgttgaca gcaaagtcat caatatcaat
     1801 ctcaatactg tcatccaaaa actcaaaacc tatttcccta tccgcactga atacaagttc
     1861 tccacataca taagctttga aaccatttgc atcttcagga aaaggaactt ttatcctttg
     1921 cttcttgttc gtctttacta caaacagctt ttccacatta tcctccagat gcaggtctac
     1981 cacaccaaag gcaatatccg ccttttcgcc acaaaacttg tattccgcat tcccaattac
     2041 gatgcctgtt gtggcgcttc ctgcaaaacc gaccagattg ccgttttcgt ccaaacgata
     2101 cgtaagcaaa tcaataccat tgtaagatat ttcgctgcca agaatgctga atttttcaag
     2161 gttcaattca acaggaggaa gaattcgtcc ttccagggct ttttcatctt ctttttcatc
     2221 tctaaagaat ttgccatccc actcttcgta aaaggttctg taatggttcg tcacagacac
     2281 actgccgttt gggaatctgc taatcaaata cctgctgtcc ttgttccttg acatgacctc
     2341 aagttcactt ccatcatagc agccaagctt ttcaagcact gaaaaaagtg tatccacatc
     2401 cctgccggta gaggtactct ggtcgtccct gagtcttgca ccaaaatata cacacaatcc
     2461 acctgatgga tatcttttga cagtacctac aataaactcg tcaagtcttg ctgcaacttc
     2521 ggtaccttct ttcggagtca caggatagac aaaatcaggc cttaagtctg tcaaaatctc
     2581 catgtccaat actccgttca atgaattttc aaaccttatc tttctgtcct tgaaagttcg
     2641 ttcaagatat gctttttcac aagcactgac accaaaaagt tcttcaaaat ccccacgaac
     2701 aagaggagag cttaaagtat caacaggatg catggaagtc catacaacct tgccgccact
     2761 atctacaaac ttcttcatta gttcaagagt ttcttttttt atgaaaggcg agaacaagac
     2821 aagcagcgtt gaatattttc ttccgctgat aaaaatatat ccgtcttcca ccttctcaaa
     2881 atgctggtgg attttctctt ctgttatgta gttgcagtag ccaaactgta ccatccagct
     2941 tccaaagcgt tcctccacat aattcagatc aagcggatac aaggcaagaa catttgtaag
     3001 tcttggagaa aacgcctgaa caaggttgtg ttcaaacctt cctgtaagat tgccgtatgc
     3061 agcacccaca ttcctaaggt gtctgagaac actatgaggg cttccccaac tgccgttgta
     3121 ggcaatggga gttttgttca gcactgccaa actgcatgca aatgcctgac tgtagtagtt
     3181 tctgtcaata aaaccgccct ctggatggtc cgtaccccct ccggttaaaa actcattcca
     3241 tctgaaatag tcat
//
LOCUS       DAONVY010000255         3250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20377_length_3250_cov_3.108920, whole genome shotgun sequence.
ACCESSION   DAONVY010000255 DAONVY010000000
VERSION     DAONVY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20377_length_3250_cov_3.108920"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2879)
                     /locus_tag="PLI11_07235"
     CDS             complement(<1..2879)
                     /locus_tag="PLI11_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPZ52695.1"
                     /translation="MKRKKFITAAVAISVAAASAVSAGMVYRHFKKTDKKDKAYSGKM
                     NFVFILGDDFGYMDTALNGSTFYETPNLQNLARDGITFTNAYAHPLCSPTRSALLTGQ
                     YPHRTMINAPRGHLEGSDEKFNVDGGASPWQEYRTKNSLNALPENLFTIGQAFQDNGY
                     FTGFIGKHHLGRGEEHQMRNRGFDVDLGMPVAGPGRYYPPYFGGFPGNVWDNGELFME
                     DEHITDRLAREADKFITDAVKKGEPFLLELWDFSVHGPFHAKKEYIEYFKNKDAPGSK
                     QENCYQMAAMIKALDDAIGKVVDSLKRNGVYDNTVIVFMGDNGGNMYDSVDGSFPTNN
                     YPLRQGKGTIYEGGIRVPCVMKVPGMTKGGVIKENIHAVDFYPTLLELADITIDPSKF
                     KNANHHCLEDRTQVLDGVSLVPLLNGTKKTLERKGIFTVFLQNVPAPGNTSAIAVNSG
                     EWKLIVNYETLQGRKERYELYNLKENISETVNLADKYPDKVEELKGYIEEHLAKTPQA
                     MPVKNNAYNSNAEVPIEYIAWDGWVKEFAKEVEKDSAIKEYPKVVIENADTEYLGKIK
                     DAVVFRIGTRSVINKGYDGKLVLVPKKDGKKIYLPADILGVKGKDGYVESGKLKKKVA
                     VYGDLIIVAGDKVNLTNEDIKRTHAYFDSFEKRDEVKWLPNMTPSFVDMEKNKMVYEQ
                     LVTAPKGLGEAIVSWQFDDGLDGFFVPVARKGNLERENGCIKFTVNETDAYIHSPEIN
                     INSVEASVLKIRFKNMSSAWEMEVYWITSSDGQYNIQKKSERIYIPSYMDDFVTYFID
                     LGKYEGWKDNIVQIRIDGIDNADSGSILYDSIELVGKSPEVTWSFEKDAEGWRAANNM
                     TFEVKDGKLGCNITGQDAYMMKTDLSVPLEKYNAVKIRYMNTTNSTRCQVYFTTTVHP
                     NIAGQFKMFTLKSNMTDFEEIVLDMSNVEKWIGNLTRLRFDPADSA"
     gene            complement(2907..>3250)
                     /locus_tag="PLI11_07240"
     CDS             complement(2907..>3250)
                     /locus_tag="PLI11_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52696.1"
                     /translation="AVFDGRYLYIRNLMPELEYKLPADLYGKDWGNLSYDAIVNAKDE
                     YPFEYDLMMSVFSRPYEELYDIDNDPGQTKNLMNEKEQLKVVEKLRKVIDMWMEETED
                     PGDPRKISRRV"
BASE COUNT          854 a          828 c          526 g         1042 t
ORIGIN      
        1 gcactgtctg caggatcaaa tctaagcctt gtaagatttc caatccattt ctccacatta
       61 ctcatgtcaa gcactatttc ctcaaaatcc gtcatattac ttttcaacgt aaacatcttg
      121 aactgtccgg caatattcgg atgaactgtt gtggtaaagt aaacctgaca tcttgttgaa
      181 ttagtcgtat tcatatacct aatctttacc gcattgtact tctcaagagg aaccgacaaa
      241 tcagtcttca tcatgtaagc atcctgtcct gtaatgttgc atccaagttt cccatcctta
      301 acctcgaagg tcatgttgtt ggcagccctc cagccttctg catccttttc aaaagaccat
      361 gtaacctccg gggattttcc gaccagctca atagaatcgt agagtattga tccagaatcg
      421 gcattatcta tgccgtctat ccgtatctgc acaatattgt ccttccagcc ttcatatttc
      481 ccaaggtcaa tgaagtaagt gacaaaatca tccatgtatg atggaatgta tatcctctcg
      541 ctctttttct ggatattgta ctggccatcc gaggaagtta tccaataaac ttccatttcc
      601 caggcactgg acatattctt aaaccttatc ttcaacaccg atgcctcaac actattgata
      661 tttatctccg gcgaatgaat atatgcatcc gtttcattaa cagtaaactt gatacagcca
      721 ttttcccgtt caaggttgcc ctttcttgca actggtacaa agaatccgtc aagaccatca
      781 tcaaactgcc aggaaactat cgcttctccc aaaccctttg gagcagttac aagctgttca
      841 tacaccattt tgttcttttc catatctaca aaagatggtg tcatgtttgg caaccacttt
      901 acctcatccc ttttttcaaa ggagtcaaaa tatgcatggg ttcttttaat atcctcatta
      961 gtaaggttga ccttatctcc agcaacaatg ataagatctc cgtaaaccgc aaccttcttc
     1021 ttaagctttc cggattccac atatccatcc ttgcctttta cccccaggat atcagctggc
     1081 agatatatct tcttgccgtc ctttttagga accagaacaa gcttgccatc ataccctttg
     1141 ttaataacag accttgttcc tattctgaac acaacggcat ccttaatctt tccaagatac
     1201 tcagtatccg cattctctat taccactttt ggatattctt ttatggcact atccttttca
     1261 acctcttttg caaattcttt cacccaccca tcccatgcaa tatattctat tggaacctcg
     1321 gcattggaat tatatgcatt attcttaaca ggcattgctt gaggagtttt agccaagtgt
     1381 tcctctatat aacctttcaa ctcttccact ttatcaggat atttgtcagc aagatttacc
     1441 gtttcagaaa tgttttcctt gagattgtaa agctcatatc tttcttttct accctgaaga
     1501 gtttcgtaat ttacaataag tttccactct cccgaattca ctgctatggc cgaggtgttg
     1561 ccaggcgcag gtacattctg caaaaagact gtaaatatgc ccttgcgctc aagagtcttt
     1621 tttgttccat ttaaaagagg gaccaacgac acaccgtcaa gaacctgtgt gcgatcttcc
     1681 aaacagtgat ggtttgcatt tttaaatttt gagggatcta ttgtaatgtc agccagttca
     1741 agcaacgtcg gatagaagtc aacagcatga atattttcct ttatgactcc tcctttggtc
     1801 ataccaggaa ccttcattac acaaggtacc cttatgcctc cttcgtaaat tgttcccttg
     1861 ccctgcctga gcggatagtt gtttgtcgga aaagatccgt caacactgtc atacatattg
     1921 ccgccgttgt ctcccataaa cacaatgacg gtattgtcat acactccgtt tcttttcaga
     1981 ctgtctacta cctttcctat cgcatcgtcc aacgccttta tcatggcggc catctgatag
     2041 cagttctcct gtttgctgcc tggtgcatcc ttattcttga agtactctat gtattctttc
     2101 tttgcatgga aaggtccatg aactgaaaaa tcccacaact caagcaggaa gggttctccc
     2161 ttcttaactg catccgtgat aaatttatcc gcttctcttg ccagtctgtc agttatgtgt
     2221 tcatcctcca tgaaaagctc accattgtcc catacattac caggaaaacc gccaaagtat
     2281 ggtggataat atctaccagg acctgcaacc ggcattccca ggtccacgtc aaaacctcta
     2341 tttctcatct gatgctcttc acccctgccc aggtgatgct tgccgataaa cccggtgaaa
     2401 tatccattat cctggaacgc ctgtccaatg gtaaacagat tttctggcag tgcattaaga
     2461 gaattttttg ttctgtactc ctgccatgga cttgctcctc cgtcaacatt gaatttctca
     2521 tcgcttccct ccaggtgccc cctgggagca ttaatcattg tcctgtgagg atactgtcca
     2581 gttagaagcg ctgaacgagt tggggaacac aatggatgag catatgcgtt tgtaaacgtt
     2641 ataccatctc tagcaagatt ttgcagattg ggcgtttcat aaaaagtact gccattcaaa
     2701 gcggtatcca tatacccaaa atcatcaccc agtataaata caaaattcat cttaccgctg
     2761 taagccttat cctttttatc tgttttctta aaatgcctat acaccatacc tgccgaaaca
     2821 gctgaagctg cagcaacaga tatggcaact gcagctgtga taaatttttt acgtttcatt
     2881 caaaaacctc ccttaatcaa ctccacttac actcttctac ttattttcct gggatctccc
     2941 gggtcttcgg tctcctccat ccacatgtca attaccttcc tgagtttctc aacaaccttt
     3001 aattgttctt tttcattcat caggttcttt gtctgaccag ggtcattgtc aatatcgtac
     3061 aattcctcat aaggtctgga aaagacgctc atcatcaaat catattcaaa ggggtattca
     3121 tcctttgcat tgactatggc atcataggaa agattacccc agtccttgcc ataaaggtct
     3181 gcaggcagct tgtattccag ttccggcata agatttctta tgtacaaata tcttccatca
     3241 aaaaccgctc
//
LOCUS       DAONVY010000256         3222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20578_length_3222_cov_4.047363, whole genome shotgun sequence.
ACCESSION   DAONVY010000256 DAONVY010000000
VERSION     DAONVY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20578_length_3222_cov_4.047363"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="fucI"
                     /locus_tag="PLI11_07245"
     CDS             complement(<1..1055)
                     /gene="fucI"
                     /locus_tag="PLI11_07245"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584160.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HPZ52697.1"
                     /translation="MKRNRYIGEYPVVGIRPVIDARRGVLKVRESLEEQTMTMARKAA
                     ELFRENLRYANGEPVKVVISDTTIGRAGEAALCDAQFKKEGVSITLTVTPCWCYGSET
                     MDMDPSTIKAVWGFNGTERPGAVYLAAVLACHAQKGLPAFGIYGREVQDKDETSIPDD
                     VKEKLLRFGRAAVAVSSMRGKSYLQIGSMCMGIAGSLINADFFEDYLGMRVESVDEVE
                     IIRRITEEIYNKEEYEKAIQWVRENCVEGFDKNPVDNQKSREEKDEEWEFLVKMMIII
                     KDLYHGNPNLPAGCEEEKAGHNAIAGGFQGQRQWTDFYPNCDFPEAMLNSSFDWEGAR
                     EPYILATENDTLNGVCM"
     gene            complement(1060..3171)
                     /locus_tag="PLI11_07250"
     CDS             complement(1060..3171)
                     /locus_tag="PLI11_07250"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPZ52698.1"
                     /translation="MECSVNKDGKRVVLTIDGKTVTPVAYMTYNPRKENFTRFSDIGV
                     NLFSFGVYLGDQGINSESGIKPFRSGFYKGYNEYDFTEVDKDLEMITSHNKGAYIIPR
                     VYLDCPQWWEKENKSELSLDAKGRPMRQSYASDKWREDALSAIYALIDHISSSQWDEN
                     VIGYQVAAGLTEEWQYHHTYPYQYLDYSNCNLKNFREWLRNKYESISDLNREWNTVYK
                     DYENIVFPNPFERSHALNGALRDPKKERHVIDFYTYHSHIMADTILFFCEKIKKHTNY
                     ERLTGAFYGYILEIMSNDYGHCGIHEVLKSEYIDFLASPNSYMDARSPGIDWPYMSVV
                     DSAKLHGKLWFIESDTRTSLTRPLKESMPHAAPDNSRYSFEGVWKGPGKNLSLSLMLK
                     GFGRVLTGGIGTWWFDMWGGWFNDPDYLGLIEKTTKILEEHIKKDVRAKAEIAVFVDP
                     ECFHYFSTNHYILHELVYSQRKSLGQMGVPYHIYLTEDLELESFDIEAYNLYIFLNNV
                     YMKDSLKECIDRKIKRNGRTLLWIYLQDMFTQSASITGFNIKYAKDSLPIQAVYKSEV
                     FPSLAVVCPEFSVADLANARVLATQKDTNIPVCTVKCFDDYNSIYCTVPNMPVSLLRD
                     IANMSRVHIYCHTNDVVYADEEYIIIHASYSGEKRIYLPVKAIVYDGLTDNKLYGPCT
                     YFDFPMEQYDTKIFRIIDCEV"
BASE COUNT          858 a          777 c          547 g         1040 t
ORIGIN      
        1 aacatgcaaa cgccattcaa cgtgtcgttc tctgtagcca ggatgtacgg ttctctcgca
       61 ccttcccaat caaagcttga attaagcatt gcctctggaa aatcacagtt tggatagaag
      121 tcagtccact gtctttgacc ttggaaacct cctgcgattg cattatgtcc tgccttttct
      181 tcttcgcagc cagcaggcag attaggattt ccatgataca agtcttttat aattatcatc
      241 atcttgacaa ggaactccca ttcctcatcc ttctcttctc ttgacttttg attatcaacc
      301 gggttcttgt caaatccttc aacacagttt tcccttaccc actggatggc tttttcatac
      361 tcttccttgt tatatatctc ctctgtaatt cgtctaataa tctccacttc gtcaacagat
      421 tcaacccgca ttcccaggta gtcttcgaag aagtccgcat ttattaatga tcccgcaata
      481 cccatgcaca ttgagcctat ctggagatag gattttcctc tcatcgagct tactgctacc
      541 gccgcccttc caaaccgcag aagcttttcc tttacatcgt caggtatgga ggtttcgtcc
      601 ttgtcctgca cttcccttcc gtagatgcca aaggcaggca aacctttttg tgcatggcat
      661 gcaagcaccg ctgccagata aacagctccg ggtctttctg taccattaaa accccatacc
      721 gctttgattg tgcttgggtc catgtccatt gtttcagaac cgtagcacca gcatggggtt
      781 accgttaatg ttattgaaac accttccttt ttaaactgag catcacacaa tgcagcttcc
      841 cctgccctgc ctatggttgt gtctgatatg accaccttta caggttcacc attggcatat
      901 cgcaggttct ctctgaacaa ttcagcagcc tttcttgcca ttgtcattgt ctgctcttca
      961 agagattccc ttaccttcag cacacctctt ctggcatcaa tgacaggcct gattccaact
     1021 accggatact cacctatgta tcggtttctt ttcattaaac tacacctcac aatctatgat
     1081 tctaaatatt tttgtatcat attgttccat cggaaaatca aaatacgtac atggtccgta
     1141 tagcttatta tctgttaaac cgtcatatac tatagccttt acaggcaggt atattcgctt
     1201 ctcgcctgaa taacttgcat gaataattat atattcctca tcagcatata ctacgtcatt
     1261 tgtgtgacaa tatatgtgaa ccctgctcat gttggcaata tctcttaaaa gactgacagg
     1321 catattgggc accgtacagt aaatgctgtt gtagtcatca aaacatttta ccgtacatac
     1381 aggaatattc gtatcttttt gtgtcgcaag aactctggca ttggccaaat ctgccacaga
     1441 aaactcagga cagacaaccg ccaaagaagg aaacacctca gacttgtaca ctgcttgaat
     1501 gggcaatgag tcttttgcat atttaatgtt gaaaccagta atagaagcag attgcgtaaa
     1561 catatcctga agatatatcc aaagaagagt tctgccgttt ctttttatct tcctgtcaat
     1621 gcattctttt aaactgtctt tcatgtatac attattcaga aatatgtaca gattgtatgc
     1681 ttcaatatcg aaagattcca actccaggtc ctcagttaaa tatatgtgat aggggacgcc
     1741 catttgtccc aaggattttc tctgactgta tacaagttcg tggaggatat aatgatttgt
     1801 tgaaaaatag tggaaacact cagggtcgac aaatacagca atctcagctt tagctcttac
     1861 atccttcttt atatgctcct ccaatatttt tgtagtcttt tcaattaatc caagataatc
     1921 aggatcatta aaccatccgc cccacatgtc aaaccaccag gttccaattc cacctgtcaa
     1981 aaccctccca aaacctttca acataaggga cagtgacaag ttcttgcctg gccctttcca
     2041 tacaccttca aatgaatatc ttgaattgtc tggagcagca tgaggcatgg actctttaag
     2101 aggtcttgta agagaagttc ttgtatcaga ttcaataaac cagagtttgc cgtgcagctt
     2161 ggcagaatcc acaactgaca tatacggcca gtctataccc ggacttcttg cgtccatgta
     2221 gctgttgggg cttgctagaa aatcaatgta ttccgatttc aaaacctcat gtatcccaca
     2281 gtgcccgtaa tcgttgctca ttatctcaag tatgtagcca taaaaagcac ctgtcagtct
     2341 ttcataattg gtgtgtttct tgattttctc acagaaaaac aatattgtat ctgccattat
     2401 gtggctgtgg tatgtataga agtctataac atgcctttct ttttttgggt ctctcaacgc
     2461 cccgtttaaa gcatggcttc tctcaaaggg attgggaaac acgatatttt cataatcctt
     2521 gtatactgtg ttccattccc tgttaagatc gctaattgat tcatacttgt ttctaagcca
     2581 ttccctgaaa ttttttaaat tacagtttga gtaatccagg tattggtacg gatatgtatg
     2641 atgatactgc cactcttctg tcaatcctgc tgcaacctga tacccgatta cattttcgtc
     2701 ccattgggaa ctagatatat ggtcaattag tgcatatatt gcgctcaatg catcttcacg
     2761 ccatttgtca gatgcgtacg actgcctcat aggccttcct ttggcatcca aagaaagctc
     2821 gctcttgttt tctttctccc accattgagg acagtcaaga taaacacggg gaatgatata
     2881 tgcacctttg ttgtggcttg ttatcatttc gagatctttg tccacttccg taaagtcgta
     2941 ttcgttgtat cctttataaa agccgcttct aaaaggcttt ataccgcttt cactgttaat
     3001 tccctgatct ccaagataaa ctccaaagct aaacaagttg acccctatat cagaaaatct
     3061 ggtaaagttc tctttgcgag ggttgtatgt catgtatgca acaggtgtta cagtcttccc
     3121 gtctattgtt aaaacaactc ttttgccgtc cttattaaca ctgcattcca tacaaaccct
     3181 cctgaaatta cagatacatt tccagtgctc caaatatatt ca
//
LOCUS       DAONVY010000257         3216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20619_length_3216_cov_3.335021, whole genome shotgun sequence.
ACCESSION   DAONVY010000257 DAONVY010000000
VERSION     DAONVY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20619_length_3216_cov_3.335021"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLI11_07255"
     CDS             <1..609
                     /locus_tag="PLI11_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52699.1"
                     /translation="ASFVSESDIFSLNYGMKTYLGRSAREWVRRGLLKNFDTNSNLYI
                     ARSGVFDELEIHDVRDVRNFCNWAYNNFDLVIVAAEEKLLSPLKNAMMLNSDYVIAVP
                     YANVDDIQLCCAYYNRLSQKGYISEENLRYVAWDYIDKVSLPESGISMIVGRSKYLGA
                     IPYDRRRLVSRNVKGSYYVNDDVCDIASSYDTVISKLMRVGG"
     gene            614..1297
                     /locus_tag="PLI11_07260"
     CDS             614..1297
                     /locus_tag="PLI11_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52700.1"
                     /translation="MKKSAFFTVAIYLASLALLAALYTFLIIPQVKKKTSLVYEEMYK
                     KSTGAFVDVLTYKGETPLMSGVVLTDVNRNNFEVVKVPANLIVENAVCDFDLIKDRML
                     KYTVVKGQQISFDLVGQDEEEFSSTRRLKEFKVKSLVGELAMKGTYVDIIARFPDGSY
                     QEVISRIRIYDILCTNVNGKCEYLKDSDGFYTIILAVNEEEFRTLNNALLTGYLDTRL
                     HLVNKVSGP"
     gene            1294..2946
                     /locus_tag="PLI11_07265"
     CDS             1294..2946
                     /locus_tag="PLI11_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52701.1"
                     /translation="MSLSRDIKWTRLLNKVSGILTDVELYMSVYHVDLKQAESAINMM
                     TELKDMAARGDADARNNIIDYYVRVLSSGKLFDVDKETVGIINFDNILDNEPQIVFEF
                     LLENRRLGEMIDKYHVENVLTWVELREIVRNEDKDDTITRRYKSKRERCIFLSILLYA
                     FAYGQDCIDSLQNQDINEIGVLNKDYVYITYKGRKIRLVFLSFDSVGTILNIQKKTTQ
                     NSSLNYDRQNPIVVTSKNNFNRISVAGYEVTPSTDDAYYNERIFNLKNITLEEMRDKY
                     NTINDGIYRFLIMNQAGRGSFFVTGSDMGVGKSTFLLSMLEKYPDSWGIGILDPQNEL
                     QVSRKYPSKNVITLVENDKKNLSECFAYLLKTSRDVLCVSEITMPEEVAELVNSALRL
                     NAGVCATMHSFSPQEVVPNLRNLMLRTSMYNDKNTAEEDIAGSIDLIIHLGRLKDGRI
                     KVMSIHEICIDREDCEFGSNVDDMREMEKKLYILGIKALKDLVLGRRYHLREIVCYDM
                     EREDWVFVEPPSDFYLDKLARYASSEVMEEGLSIFEGSHVNG"
     gene            2939..>3216
                     /locus_tag="PLI11_07270"
     CDS             2939..>3216
                     /locus_tag="PLI11_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52702.1"
                     /translation="MVEYLPLVFICTTSGVMVISYGVYHAVKFKDRVKYGNVDSKLLF
                     EKRVDSMCTFLARSRFLARLMKDLSLKLSIFNAKSYAVNMRYATLFLIL"
BASE COUNT         1008 a          488 c          823 g          897 t
ORIGIN      
        1 gcgtcctttg tgtcggaaag cgatatcttc agcctgaatt atgggatgaa gacgtatctt
       61 ggaagaagtg caagagaatg ggtcagacgc gggcttttga agaactttga tacaaacagc
      121 aacttataca ttgcgcgaag tggcgttttt gacgaactgg aaatccatga tgtcagggat
      181 gtaaggaact tctgcaactg ggcatacaac aattttgatt tagtcatagt agcggcggaa
      241 gaaaaactat tgtcaccttt aaagaatgca atgatgctta attcagatta tgtaattgca
      301 gttccctatg ccaatgttga cgacatacaa ttgtgctgtg catattataa caggttgtcg
      361 caaaagggat acatatcgga ggaaaattta aggtatgtgg cctgggatta catagacaag
      421 gtaagtcttc ctgagtcggg tatttccatg attgtgggta ggagcaagta tttgggcgca
      481 attccatatg acagaagaag acttgtttca agaaatgtca agggaagcta ttatgtaaat
      541 gatgatgtat gcgacattgc gtcaagctat gatactgtaa tttcaaagct tatgcgggtg
      601 ggaggttgat tgtatgaaga agagtgcttt ctttaccgtg gcaatttatc ttgcatctct
      661 tgctcttttg gcggcgttgt acaccttttt gataattcct caggttaaga agaaaacatc
      721 ccttgtgtac gaggagatgt ataaaaagag tacgggcgca tttgttgatg tgttgacata
      781 caagggagaa acccctctta tgtcaggagt ggttctaact gatgtgaaca ggaataattt
      841 tgaggttgtt aaagtgcccg caaacctgat cgttgagaat gctgtttgtg attttgattt
      901 gataaaagac aggatgctga agtatacggt tgttaaaggg cagcagatat cttttgacct
      961 tgtaggacag gatgaggagg agttctcaag cactagaaga cttaaggaat tcaaagttaa
     1021 aagtctggtg ggagaacttg caatgaaggg aacctatgtg gacataattg caagattccc
     1081 ggacggttcc taccaggaag tcataagcag gattaggatt tatgacatat tgtgtaccaa
     1141 tgtaaatggc aaatgtgagt atttgaagga cagtgacgga ttttacacca taattcttgc
     1201 agtgaatgaa gaggagttca ggacgctgaa caatgctctt ttaacaggat atttggatac
     1261 aaggctgcat cttgtaaaca aggtgagtgg tccatgagtc tttcaaggga tataaagtgg
     1321 acaaggcttc ttaacaaggt ttcgggaata cttacggatg tggagttgta tatgtccgtt
     1381 taccatgtag atttgaaaca ggcggagtct gccataaata tgatgacaga gcttaaggat
     1441 atggctgcaa gaggagatgc ggacgcaagg aacaatataa tagactatta cgtgagggtg
     1501 ttgtcatccg gtaaactatt tgatgtggac aaggaaacgg tggggataat taactttgat
     1561 aacattctgg acaacgaacc gcagatagtg tttgagttcc tcctggagaa tagaagattg
     1621 ggagaaatga ttgacaaata ccatgtggaa aacgttctta cctgggtgga gctaagggag
     1681 atagtaagga atgaggataa agatgacaca ataacaagga gatacaaaag caaaagggaa
     1741 agatgcatat ttttatccat tcttttgtat gcgtttgcct atggacagga ctgcatagac
     1801 agtttgcaga accaggatat taacgaaata ggtgtcctta acaaggatta tgtttatatt
     1861 acatacaagg gtagaaagat aagacttgtg tttttgtcct ttgacagtgt cggaaccata
     1921 ctgaacattc agaagaaaac aacgcagaac tcatcactga actacgacag acaaaatcca
     1981 attgttgtaa cttccaagaa caattttaac agaataagtg tggcaggata cgaagtaact
     2041 cccagtacgg atgatgcgta ctacaatgag agaatattca atttgaagaa tattactctt
     2101 gaagaaatga gggacaagta taacacaatc aatgatggga tttacaggtt ccttattatg
     2161 aatcaggcgg gaagaggcag cttttttgtc accggctcag acatgggagt gggaaagagc
     2221 acttttctac tttccatgct ggaaaagtac ccggattcct ggggaattgg aatactggac
     2281 ccacagaatg agctgcaggt aagcaggaaa tatccgtcaa agaatgtaat tactcttgtg
     2341 gagaatgaca aaaagaacct ctccgaatgc tttgcgtatc ttttaaagac atctagagat
     2401 gtcctttgtg tcagtgagat tacaatgcct gaagaggtgg cggagcttgt aaacagtgca
     2461 ttgaggttga atgctggcgt ttgtgccaca atgcattctt tttcaccgca agaggttgtt
     2521 ccaaacctac ggaatctcat gcttaggaca tccatgtaca acgacaagaa tacagcggaa
     2581 gaggatattg cgggcagtat tgatttgatt attcatctgg gaaggctgaa ggatgggagg
     2641 attaaggtaa tgagcataca tgagatatgt atagacagag aggactgcga gttcggctca
     2701 aatgtggatg acatgcggga gatggagaaa aagttgtaca ttcttggtat aaaggcgtta
     2761 aaggatctgg ttcttggaag aaggtaccac cttagggaga tagtttgtta tgacatggaa
     2821 agggaggatt gggtgtttgt ggagcctcca tcagacttct atcttgacaa gctggcaagg
     2881 tatgcaagtt cagaagtaat ggaagaaggg ttgagtatat ttgaagggag tcatgtgaat
     2941 ggttgagtat ttgcctttgg tattcatatg caccacaagt ggagtgatgg taatttcata
     3001 tggtgtatat catgcagtca agttcaaaga cagagtaaaa tatggaaatg ttgattcaaa
     3061 actcctgttt gaaaagagag ttgacagtat gtgtactttt cttgccagat cgagattcct
     3121 ggcaaggctg atgaaagatc tgtctctaaa actctccata ttcaatgcca aatcctatgc
     3181 agtgaacatg agatatgcta cgttgttttt gattct
//
LOCUS       DAONVY010000258         3212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20639_length_3212_cov_3.339880, whole genome shotgun sequence.
ACCESSION   DAONVY010000258 DAONVY010000000
VERSION     DAONVY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20639_length_3212_cov_3.339880"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLI11_07275"
     CDS             complement(<1..1001)
                     /locus_tag="PLI11_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPZ52703.1"
                     /translation="MQKFHEIEYVRPDLEKLSDAYLKGVKKLEEAQTIEEAKEGIIEC
                     EETSNELYTAHTVAYVRNTMNTQDEFYESEIEFFNNEYPKLIPLFKQYRKALLNSRFR
                     EDLEKDFGKQLFRINEAEEKTQDEAIIDDLVEESRLGVEYQKLAASCSTEFMGETCNF
                     YGLLKHMQNPDRNVRREAYLAWASLYEGISQDLDRIYDELVKVRVEMARKLGFDNYTA
                     LGYLNNKRMDYTREDVAKFRQQVKRVIVPFCEKIRKEQAKRIGVDKIKYYDESFMFPE
                     GNPNPVGNKDQMIAWAQEMYNEMSKETGEFFNFMVEYDLFDLETRPGKHLGGYCTVMP
                     "
     gene            complement(1018..2907)
                     /locus_tag="PLI11_07280"
     CDS             complement(1018..2907)
                     /locus_tag="PLI11_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52704.1"
                     /translation="MRYWCNKKEDDGSIHAIGNGRMIVYGRGPNINQVQGPPYSSPVY
                     GSFEINEPDRNIESRSQRAPRDSKWIHNIYEDGKQIGIFKDFMDPEYNVFVRSFEMEK
                     SICIKPVLFPHSKVYYYSDYDFGNGVRKECVMLVMTIGTRYFTNFALMDELRMYILWD
                     GDAKYIKEENSFVLEKGSGQLALVSDHPKKIDKHLSFYFSGADIEERSGHYFADYLKK
                     GDRILESVPEDHPEAKRIKDVLESVAVLTKCQQSYDGGILAGHYYPLAYVRDMAGTMR
                     SLVKSGYVEEGKNVLEFWFNKWKHFGNLVNAESMGHNQDRLLFSNDEVEVPAYIVYCA
                     MYYYNATKDKDFLIEINDMLRWAFSVQMPHLVKGMTSFSGDETYIAGFVFPRPFIYHG
                     SAESTLLFIEGGRRFLDFCREEGLLDDEEMSRYESAVKEASDLYKKNFVIDGVIHGNN
                     PEREKGFEKPRVHRGFCDVDQLLERPAPLMWLERGEHGYYRCPDCFDEKIDMILDPSK
                     RYILGSTGLLPVYYDSDLFSDDEIRTNVKRFARLFKEKGFIPSNVEGNRSLGYDFGLL
                     LCNLAYLDVPLKEEVLKVMLDIVDDTGAWVEYYDDKVPFNCRCRPWESGINIEALIYY
                     LEKTK"
     gene            complement(2912..>3212)
                     /locus_tag="PLI11_07285"
     CDS             complement(2912..>3212)
                     /locus_tag="PLI11_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52705.1"
                     /translation="INVDFFARLFEEKGYIPSHAEGTRSLGYDFGLLLYNLAYLDDPL
                     KEEVLKVMLDIVDGTGAWVEYYDNKVPFNCRCRPWESGINIEALIYYLEKLQQGE"
BASE COUNT          886 a          798 c          505 g         1023 t
ORIGIN      
        1 ggcatgaccg tgcagtaacc acccaggtgc ttcccgggtc tagtctcaag gtcaaacagg
       61 tcatattcca ccatgaagtt gaaaaactcc cctgtttcct tgctcatctc attgtacatc
      121 tcctgagccc atgcaatcat ctggtccttg ttgcccacag ggttcggatt gccttcaggg
      181 aacatgaaac tttcatcgta atactttatc ttgtccacac ctatccgctt tgcctgctcc
      241 tttcttatct tttcacagaa aggaacaatc acccttttta cctgctgtct gaattttgca
      301 acatcttccc ttgtataatc catcctctta ttattcagat aaccaagagc tgtgtaattg
      361 tcaaagccca gttttcttgc catttcaacc ctgactttta caagttcgtc gtaaatcctg
      421 tcaaggtcct gggatattcc ttcataaaga cttgcccaag ccaaatacgc ttctcttcta
      481 acatttctgt caggattctg catgtgtttc agaagcccat agaagttaca tgtctccccc
      541 ataaattctg tactgcagga agcggcaagc ttctggtatt caacacccag tcttgattcc
      601 tccacaagat cgtcaataat cgcttcatct tgtgtttttt cctcggcttc attaattcta
      661 aaaagttgct ttccaaagtc cttttccaag tcttctctga acctagagtt aagaagtgcc
      721 tttctgtact gcttgaataa gggaatcagt tttggatatt cattgttgaa aaactcaatt
      781 tcactttcat aaaactcgtc ctgagtattc attgtatttc taacatacgc aacagtgtgt
      841 gctgtataca actcattgga tgtttcttca cactctataa taccttcctt tgcttcttca
      901 atagtttgag cttcttcaag tttttttact ccttttaaat atgcatcgct gagcttttca
      961 aggtcaggtc taacatattc tatctcatga aatttctgca taaacaacac acctttctca
     1021 ttttgttttt tccaaataat atattaatgc ctcaatatta attccacttt cccacggcct
     1081 gcatctgcag ttgaaaggaa ccttgtcatc atagtattct acccatgctc ctgtatcatc
     1141 cacaatatcc agcatgactt taagcacctc ttccttgaga ggaacatcaa gatatgccaa
     1201 attgcagagc aaaagtccaa agtcatatcc caacgagcgg tttccttcca catttgacgg
     1261 aatgaatcct ttttccttga atagacgagc aaatctttta acatttgtcc ttatctcatc
     1321 atcagagaac aaatcactgt cgtaatagac aggcaacaaa cctgtagaac ctaaaatata
     1381 tctcttgctt gggtctagaa tcatatctat tttctcatca aagcagtcag gacatctgta
     1441 atatccatgt tcgcctctct caagccacat tagaggagca ggtctttcaa gaagctggtc
     1501 cacatcgcaa aaacctctgt gaaccctggg tttttcaaac cctttttccc tctccggatt
     1561 gtttccatga atgactccat ctattacaaa gttctttttg tacaaatcac tggcttcttt
     1621 gacagcactt tcataccttg acatctcctc atcatccaaa agcccttctt ctctgcaaaa
     1681 atccaaaaag cgtcttccac cttcaataaa cagcaaagtg gattcagcag aaccgtggta
     1741 aataaaaggt ctggggaata caaagcctgc aatatatgtt tcatctccgc tgaaactggt
     1801 cattccctta accagatgag gcatttgcac actgaaagcc catcttagca tatcattgat
     1861 ttcaattaaa aagtctttgt ctttcgttgc gttgtaatag tacatggcgc agtaaacaat
     1921 gtatgcaggt acttccacct catcattcga aaatagtagc ctgtcctgat tgtgtcccat
     1981 gctttctgca ttcaccaaat ttccaaaatg tttccacttg ttgaaccaaa attcaagcac
     2041 atttttgcct tcttccacat accccgactt aacaagtgaa cgcatggttc cagccatgtc
     2101 ccttacatac gccagtggat agtagtgtcc tgcaagaata cctccatcat aggattgctg
     2161 gcactttgtc aaaacagcaa cgctttcaag gacatccttt atcctcttcg cttcaggatg
     2221 atcctctgga acagactcca aaatccggtc tccttttttg aggtagtcag caaagtagtg
     2281 accgcttctt tcctctatat ctgcaccgga gaaatagaag gaaagatgct tgtcaatttt
     2341 cttgggatgg tcagaaacca aagccaattg tccggaaccc ttttctaaaa caaacgaatt
     2401 ctcttcctta atgtattttg catctccatc ccagagaatg tacattctta gctcgtccat
     2461 taaggcaaaa ttcgtaaagt accgagtccc tattgtcatc accaacatga cacattcctt
     2521 cctaactccg tttccaaagt cgtaatcgct gtagtagtat actttggaat gaggaaataa
     2581 aacaggcttt atgcaaatac ttttctccat ctcaaaagag cggacaaaca cattgtattc
     2641 aggatccata aaatccttaa aaatgccaat ctgttttcca tcctcatata tattatgaat
     2701 ccacttcgag tctctgggtg ccctttggga ccttgactca atgtttctat caggttcatt
     2761 tatttcaaaa gagccataaa ctggtgatga atatggagga ccttgtacct gattaatatt
     2821 cggacctctc ccataaacaa tcattctgcc gttgcctatg gcgtgaatac ttccgtcatc
     2881 ttccttcttg ttgcaccagt atcgcatttt actattctcc ttgttgcaat ttttccagat
     2941 agtagattag cgcctcaata tttataccac tttcccaagg tctgcatctg cagttgaacg
     3001 gaaccttgtt gtcataatat tcaacccagg ctcccgttcc atccacaata tccagcatga
     3061 ctttcaacac ctcttccttg agaggatcgt caagatatgc aagattatac aaaagaagtc
     3121 caaagtcgta tcccaatgaa cgggttcctt cagcatggga tggaatatat cctttttcct
     3181 cgaaaagacg ggcaaagaaa tctacattta tc
//
LOCUS       DAONVY010000259         3197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20762_length_3197_cov_2.598663, whole genome shotgun sequence.
ACCESSION   DAONVY010000259 DAONVY010000000
VERSION     DAONVY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20762_length_3197_cov_2.598663"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLI11_07290"
     CDS             complement(<1..233)
                     /locus_tag="PLI11_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52706.1"
                     /translation="MRIKRILLLILIGMVSLVFISCNKQNTPGTTKEPQNTPSETKEV
                     TTTKEPEETPEPTESLPKLDESQATYLTTLHDGT"
     gene            complement(251..2080)
                     /locus_tag="PLI11_07295"
     CDS             complement(251..2080)
                     /locus_tag="PLI11_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52707.1"
                     /translation="MKRIVTFIAAFILLAALIGCNKEDNDLIDNKSDGGMLAYAGVDD
                     LGRVLGMPADKDIPEYNKDKLVGMFYFINHGGKTTQGPYDISKILEKDPEAFKSDERW
                     INAGGGAVNTIHWWGESIFGYFRGSDKWVKQRDVMLLTDAGVDFLILDSSNGATYKEH
                     VLVLLEVLDEFYKQGYKVPKVAFVTKHNPGTHMMEIFNEIYKAHSEYSHLWFELDGKP
                     LILGKTDDPAFTDEFKEFFTIRYSQWPREAKREDGFPWMAFERPQHLFGKELGTTIIS
                     VSVAQHTGSLAFSNGAFHGDETVRSRSFHNGKEDKSEDAYLHGYNFAEQFEYAISVDP
                     DIIFVTGWNEWIAYRLNEWYDVTEQRYNDVAIFVDAATANYSRDIQPMKGGYGDNYYM
                     QLIYYIRKFKGAAQVNNKELEENKEITHSSVDIKGDFSQWDDIGTSYVDYAGDTMHRN
                     ARGFGKIVYVNETGRNDIVLSKVTHDGENVYFYVETKEPLSPYTDEHWMNLFIKTGNN
                     EDNWYGYDFVINRQILSNTKTVVEKCKGGWDWEKVSEIEYKAEGNKLMFSIPKNALGL
                     DIGSEINIEFKWSDNYQGEGDFWSFYLDGDTAPYGRLNYVFVN"
     gene            complement(2082..2969)
                     /locus_tag="PLI11_07300"
     CDS             complement(2082..2969)
                     /locus_tag="PLI11_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52708.1"
                     /translation="MFKFKNIAFYVLVIVILFAFANGVTASSKDLIYVDDFASNQANP
                     KAIFRFNAWHKIEDEQLYMGFNTNDNDWGEAAEAYIANEKFEFDEEHEKYEYVVKFKS
                     NGFRNDLWYSCMVGIRVAGNDKNNFKPADSNSGLYIGIAHTNKAVVYHGGGGWPGGAF
                     TVDIPANFAETNTLYIVDDNDIISYYMDTPEETRVLFLSIKISEDKLVAYDKDSNVIY
                     EADNNLKDQVGYFKIFNHSTKTIVDSIEIYNVATAPEPEPENPETGDNSFMAVLSMII
                     AAFVLFAVFMRLKNVSERC"
BASE COUNT          893 a          736 c          536 g         1032 t
ORIGIN      
        1 gtgccgtcgt gcagcgtggt aagatatgta gcctggcttt catcaagctt tggtaaactt
       61 tctgtaggtt cgggagtctc ttcaggctcc ttggtggttg taacttcttt agtttcagaa
      121 ggagtattct gcggctcctt cgttgtaccg ggtgtgtttt gcttgttgca gctaataaac
      181 acaagagaca ccattccaat caatattaaa agcaaaatac gttttatcct catatttgtt
      241 tttacctcct tcagttcacg aaaacatagt taagtcttcc gtaaggtgcg gtatcgccat
      301 cgagatagaa ggaccagaaa tctccttctc cctgatagtt gtcgctccac ttgaactcaa
      361 tgtttatttc acttccaatg tccaaaccaa gtgcattctt tggaattgag aacataagct
      421 tgttaccttc tgccttgtac tcaatttcgg ataccttttc ccaatcccaa ccgcctttgc
      481 atttctcaac aacagtttta gtattggaaa gaatctgacg atttataaca aagtcatatc
      541 cgtaccaatt gtcttcgttg tttcccgttt taataaacag gttcatccaa tgttcgtccg
      601 tatacggact caaaggctct ttggtttcaa catagaaata tacattctca ccatcatgtg
      661 taaccttgct aagtacaata tcgtttctac ccgtttcatt gacatataca atttttccaa
      721 atcctcttgc atttctatgc atggtgtctc cagcataatc aacgtatgaa gtcccaatat
      781 cgtcccactg ggaaaaatcc ccctttatgt ccacacttga gtgtgtaatt tccttgtttt
      841 cctcaagctc cttgttattt acctgcgcag cacccttgaa ctttcggata tagtagatta
      901 gctgcatata gtagttgtca ccgtatccgc ctttcatggg ctgaatatct cttgagtaat
      961 tggcagtggc tgcatcaaca aaaatagcca catcattgta cctctgttcg gtgacatcgt
     1021 accactcatt aagtctatag gctatccact cattccatcc tgttacaaag ataatatcag
     1081 gatccacgct tatagcgtat tcgaactgtt cagcaaagtt gtatccatgc aagtacgcat
     1141 cttcggattt atcttccttg ccattatgga agcttctgct tctaacagtt tcgtcaccgt
     1201 ggaaagctcc gtttgagaac gcaagcgaac ctgtatgctg tgccaccgat acactaatta
     1261 tcgtagtacc aagctccttg ccaaaaagat gctggggacg ttcaaaagcc atccagggaa
     1321 aaccgtcttc ccttttcgct tcccttggcc attgtgaata cctgattgta aagaactctt
     1381 taaattcgtc ggtaaaggca gggtcatcag tcttgcccag aatcaaaggc tttccatcca
     1441 attcaaacca caaatggctg tactcactat gagctttata tatctcattg aaaatctcca
     1501 tcatgtgggt tcccggatta tgctttgtaa caaatgccac cttcggtacc ttataacctt
     1561 gcttataaaa ttcgtccaac acttcgagca agacaagtac atgttctttg tatgttgcgc
     1621 catttgacga atcaagtatg agaaaatcaa cacctgcatc cgttaaaagc atgacatccc
     1681 tttgctttac ccatttgtcg gatcctctga aatagccaaa tatggattct ccccaccagt
     1741 gaattgtatt aacagcgcct ccacctgcat taatccagcg ctcgtcggat ttaaaagctt
     1801 ctgggtcctt ctcaagaatt tttgaaatat cataaggtcc ttgtgttgtt tttccaccgt
     1861 ggttgataaa atagaacatg ccaaccagtt tgtccttgtt gtattcaggt atatccttgt
     1921 ccgcaggcat gcccaacact ctgccaagat cgtcaactcc ggcgtaagcc agcataccac
     1981 catcactttt attatctatc aaatcattat cctccttgtt gcatccaatc aaggcagcca
     2041 ataatataaa tgctgctata aacgttacaa ttcttttcat cctaacacct ttcacttaca
     2101 ttttttaaac gcatgaatac tgcaaaaagt acaaaagctg caattatcat ggacaaaaca
     2161 gccatgaaag agttgtcacc tgtttcagga ttttcaggct ccggctccgg agcagtagcc
     2221 acattgtaga tttcaatgga gtcaactatg gtttttgtac tgtgattgaa tatcttgaaa
     2281 tatccaacct gatctttcaa attgttgtcg gcttcataga taacattgct gtccttgtca
     2341 taagcaacaa gtttgtcttc tgaaattttt atgctcaaga acaacactct ggtttcttct
     2401 ggtgtatcca tgtaatacga gattatatca ttgtcatcaa caatgtaaag ggtattggtc
     2461 tctgcaaagt tagccggaat gtcaacagta aaagctcctc caggccagcc accgcctcca
     2521 tgatagacaa cagccttgtt ggtgtgggca attcctatat acagtccaga gttggaatcc
     2581 gctggcttga aattattttt gtcgttgcct gcaaccctaa tacccaccat gcatgaatac
     2641 caaaggtcat ttctgaaacc gttactcttg aatttgacaa catactcata tttctcatgc
     2701 tcctcgtcaa actcaaactt ttcgttggca atatatgctt ctgcggcttc tccccagtca
     2761 ttgtcgttcg tattgaagcc catataaagc tgctcatcct caatcttgtg ccaagcatta
     2821 aagcggaata tggctttagg atttgcctgg ttacttgcaa agtcatcaac atatatcaaa
     2881 tctttggatg atgctgtcac accatttgca aaagcaaaaa gtatgactat tacaagtaca
     2941 taaaaagcaa tatttttaaa tttaaacata tctatttctc tccttttaat ttaactttct
     3001 caagattaac aaaacgtaaa tcttttgtct tctgatatat tgagtgtatt ttagatatat
     3061 tgacatttct tttaatattc tgtattattt aaaacatcta cagcgaatat ggatgtgaaa
     3121 attatgtcag aaaactctaa ctccggctat attttatacg tatcaaacgc caaatcagat
     3181 acaagccaaa taatcaa
//
LOCUS       DAONVY010000260         3185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_20854_length_3185_cov_2.846326, whole genome shotgun sequence.
ACCESSION   DAONVY010000260 DAONVY010000000
VERSION     DAONVY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20854_length_3185_cov_2.846326"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLI11_07305"
     CDS             <1..266
                     /locus_tag="PLI11_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52709.1"
                     /translation="SARTYAESTIVDIGKKEEVHLPGMVGIFPLVDKDNKPRSYRPDP
                     VFRFKRWEEHETAVVEFSWTTVNDDEFSGVYAFNLKTGEITLV"
     gene            354..1457
                     /locus_tag="PLI11_07310"
     CDS             354..1457
                     /locus_tag="PLI11_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPZ52710.1"
                     /translation="MHMYIPWERIEDFIIRAFVKLGVPEEDAAICADVLIESDKRGIE
                     SHGVNRFKPIYVDRIMDGILNPVTEFEVVRETKTTAVVDGHNGMGMVVAYKAMQMCIE
                     KAKEYGMGMVAVRNSTHYGIAGYYATMATDADMIGITGTNARPSIAPTFGVENMLGTN
                     PLTFGMPTDEEFPFVLDCATSITQRGKIEYFARNNMETPYGMVIGEDGKAMTDSKEIL
                     KALSEGRAALAPLGGIGEELGGYKGYGYATAVEILSAALQAGSYLKMLGGVNEKGEKI
                     PYPLGHFFIAIDPEAFMGAEVFKKIAGNILRELRNSKKAEGHDRIYTAGEKEYLAKME
                     RKEKGLYVNESVCKDLLEVRDMLNLDFAFPFEK"
     gene            2061..>3185
                     /locus_tag="PLI11_07315"
     CDS             2061..>3185
                     /locus_tag="PLI11_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPZ52711.1"
                     /translation="MKCESCGYELDGDFEFCQNCGQELSKCEMCKTYIPKDKKLCTRC
                     GSELKEKLNKSKRNLKIASVATVVLYLIAVIGFAVFWGFTALVDYPNLVVYNKDGLVH
                     IANFDLTRASKEDALIKPVNTRISQDGSKFFYINENVNSLMYVELPALRKGGVVDDSV
                     KEFKISKTGDLVYYVKLSPHERKLYKSDLTSPELIAEGVVDFKISPDGSKLLYTTEDE
                     VFYYKDDNLEFSSAYDTNHPLYFDVELDCICFVLDGKLNTYKDGAVVEVYDGVKKMVS
                     CLQDGRLYFHNKANSLCYYDLKEVKVLNLYYSSLLYAGTKEPVVIVDSANDATVVHGD
                     RTITLSINSTPVKASASGKKVYVLSWDGHLYSRDLEEGRWE"
BASE COUNT         1033 a          525 c          758 g          869 t
ORIGIN      
        1 attctgcaag aacatatgct gaaagcacta ttgtagatat aggtaaaaag gaagaagtgc
       61 atttgccagg catggttgga atctttccgc ttgttgacaa agacaacaag ccaagaagtt
      121 acagacccga tcctgtcttc aggttcaaaa gatgggaaga acacgaaacg gcagttgtag
      181 agttttcgtg gacaacagta aatgatgatg agttcagtgg cgtgtatgcc ttcaacctga
      241 aaaccggtga aataaccctt gtataaccta acatatgtac aattgcattt cggtcataat
      301 tatgatacat ttttatacaa tagttaatgg tttaaaacct aataaaggag cggatgcata
      361 tgtacatacc ctgggaaagg attgaagact tcataatacg cgcttttgta aaacttggcg
      421 tacctgaaga agatgcggca atctgcgctg atgtacttat tgaaagtgac aagaggggaa
      481 tagagagtca tggcgtaaat cgcttcaaac ccatatacgt agacaggata atggatggaa
      541 tattgaatcc ggttacagag tttgaagtgg taagggagac caaaaccact gccgttgttg
      601 acggacataa cggcatggga atggttgttg catacaaggc aatgcagatg tgtattgaaa
      661 aggcgaaaga gtatggaatg ggaatggtgg ctgtaaggaa ctcaacccac tacggtattg
      721 caggatacta tgccaccatg gcaacggatg cggatatgat tggcataaca ggtaccaatg
      781 caagaccttc catcgctcct acctttggag tggaaaacat gctgggcaca aatcctctta
      841 cctttggcat gcctacggat gaagaatttc cgtttgtgct tgactgtgcc accagtataa
      901 cccaaagagg gaagatagaa tactttgcca gaaacaacat ggaaactcct tatggcatgg
      961 taattggtga agatggcaag gcaatgacag acagcaagga aatattgaaa gcgctttctg
     1021 agggaagggc agctttggct cctttgggag gaatcggcga ggagcttgga gggtacaagg
     1081 gctatggtta tgctaccgct gtggagatac taagtgcagc gctccaggca ggcagctatt
     1141 taaaaatgct tgggggagta aatgaaaagg gagaaaagat accttatcct ctaggtcatt
     1201 tctttatagc tatcgaccct gaggctttca tgggagctga agtattcaag aagattgcag
     1261 gaaatattct aagagaactt aggaattcca aaaaggccga aggtcacgac cgaatctata
     1321 ccgcaggaga aaaggagtat cttgccaaga tggagagaaa ggaaaaaggt ttgtatgtta
     1381 acgaatcggt ctgtaaggat cttcttgaag tgagagatat gctgaatctt gattttgcat
     1441 ttccttttga aaaataacat gagatgaatt tcaaggagaa aaaatgcttt acggtagttc
     1501 tggatgcaga aaaaagctaa aaaagattgc tgccgctatg gcagcactgt ttttttgcct
     1561 ctgctttagt gtttttcatc ttcgacaaag gaactgatgt tgtaatatat cagaaggata
     1621 atatcaatag cctttttaaa tcaggagggg gagtgtattt ctttaacgga aggcagagca
     1681 gataaattga tgattcagta gacagagtgc ttatgccaga aagagaatac tattgtgact
     1741 ttgactggaa cgtgtatttc attatatcgg atccgatgat ttgattattg aaagaagtct
     1801 tttgtaaaaa tatcttttaa atggtagaat tatgcatatg ttaaaggaat acatgctgga
     1861 agaaacaatt gagaaaaaga gaagtttcaa caaaaaaata ttacttgtta ctgttatagt
     1921 tttgattctg gcataagctt ttcttctctt taaacgaaga ccaactgttg tggttgtttt
     1981 aaagcatgca ttgttgactg tatatgtaat atatactatt attttcatat caaaaaacat
     2041 aaagagaggc caatttaatt atgaaatgtg aatcatgtgg ttatgaactg gatggtgact
     2101 ttgaattctg ccagaactgc gggcaggagc tttcaaagtg tgagatgtgc aagacatata
     2161 ttccaaagga caaaaagctc tgcacaagat gtggcagtga acttaaagaa aagctgaaca
     2221 agagtaaacg gaatttaaaa attgcatctg ttgcaacagt agttttgtac ctgattgcag
     2281 ttattggatt tgctgtcttt tggggtttta ccgcccttgt tgattatccc aatcttgttg
     2341 tatacaacaa agacggtctt gttcacatcg caaattttga tctcacaaga gcaagtaaag
     2401 aagatgctct gataaaacct gtgaatacaa gaatcagcca ggatggaagc aaattcttct
     2461 atataaacga gaatgtcaat tctttaatgt atgtggaatt gcccgctttg cgaaagggcg
     2521 gagtggtgga tgacagtgtc aaggaattca agataagcaa aacaggcgac ttggtctatt
     2581 atgtgaaact aagcccccat gaaagaaaac tgtataaaag tgatttgaca tcacccgaac
     2641 tcatcgctga aggggttgtg gattttaaaa tatctcctga tggaagcaaa ctgctttata
     2701 caacggaaga tgaagtattc tactacaagg atgacaatct tgagttttcc tctgcctatg
     2761 acacaaacca ccccctatac ttcgacgtgg agcttgactg catctgcttt gtattggatg
     2821 gaaagcttaa tacatacaag gacggagcag ttgtggaggt atatgacggg gtaaagaaaa
     2881 tggtaagttg tcttcaggat ggaagactgt atttccacaa caaggccaac tccctctgct
     2941 attatgacct gaaggaagta aaagtgttga acctctatta cagttcactg ctttatgcag
     3001 gcacaaaaga acctgttgtg atagtggaca gcgccaacga tgccactgtt gtacatgggg
     3061 acaggaccat cacattatct ataaacagca ctcccgttaa agccagtgca agcggtaaaa
     3121 aggtttatgt tttatcctgg gacggacatc tgtattctag ggatttggaa gaaggcagat
     3181 gggaa
//
LOCUS       DAONVY010000261         3149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21147_length_3149_cov_3.176794, whole genome shotgun sequence.
ACCESSION   DAONVY010000261 DAONVY010000000
VERSION     DAONVY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21147_length_3149_cov_3.176794"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..825)
                     /locus_tag="PLI11_07320"
     CDS             complement(97..825)
                     /locus_tag="PLI11_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPZ52712.1"
                     /translation="MAGHSKWANIKHKKSKTDAQRAKIFTKLGREIQVAVREGGPDPE
                     TNSRLKDVIAKAKANNMPYDNIQRSIKRASGEGMSDNMEEITYEGYGPSGVAVIVEAV
                     TDNRNRTAGEMRHYFDKYGGNLGATNCVSFMFDRKGIIVIEDDGSFEEDTLMMDVLET
                     EAEDFSNIDGAYEIATAPSSFSSVRDVLEKKGYKFAEASITMVPQSYVKLENEEDREK
                     FAKLIDALEDNDDVIEVYHNYSEE"
     gene            complement(886..1512)
                     /locus_tag="PLI11_07325"
     CDS             complement(886..1512)
                     /locus_tag="PLI11_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPZ52713.1"
                     /translation="MSRSYITLSKEGSGTYVEKRSEFIGYAKPVSTEQECIDYIEKIK
                     GMNKGARHNVYAYIVNEDGQVVSRYSDDGEPQGTGGIPVLDVLRKQDITNAVIVVTRY
                     FGGVLLGAAGLVRAYGKAASLAVADAGLCRMVLCQPVSVTVEYTFYGKLQNALADKMA
                     QAPVFGENVTVTYIVEEDEVDKVVNIVNEVTNGKNYLSLGEKEYYPVT"
     gene            complement(1509..>3149)
                     /gene="hflX"
                     /locus_tag="PLI11_07330"
     CDS             complement(1509..>3149)
                     /gene="hflX"
                     /locus_tag="PLI11_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPZ52714.1"
                     /translation="YINRRNRVEAIVIGDDRSVSLPFMDNRRGTNRLSGIRFLHTHPG
                     GSSLPSDVDLNSLKTLRLDGMIVIGVVNYNDKPHVTGVSVTMLERDEEERLSNTKLYG
                     PYSMKELDKFDELFEIVEETDVITYTIGEVTQEEQERAILVGVSTREDEAYLDELAEL
                     ARTAGALVVGRFVQKRASIDARYYIGKGLAEELAFHRQSLNADLVIFDDELSYAQIRN
                     LEEITGVKIIDRTALILDIFAKRATSLEGKLQVELAQLQYRLPRLTGMGQILSRLGGG
                     IGTRGPGETKLETDRRAINRKINYLQRRLKEIASKREVLRKERIKNEVPVISLVGYTN
                     AGKSSLMNCLCEAGVFVEDKLFATLDSTVRKMITEDNSTYLLVDTVGFIKKLPTHLVE
                     AFKSTLEEATQADLLLHVVDATSPDLDERIEVVESILKDIGAANKPKYLVVNKMDIVS
                     DGGVVKPGRVFAKTFYVSARTGEGLDELKKGIFDFFTRFFEEFDILVPYKEAWVLPFL
                     HEKGVVKDEQYTEDGIKVTGTLPNQYMHKIREFERGNV"
BASE COUNT          834 a          838 c          503 g          974 t
ORIGIN      
        1 aaagcttatt ccggctatga ttgcgcctaa taacaataaa agtattttct tcaaactaca
       61 gccctccgat ataaattatt cctaattgta taagaactat tcctcgctgt agttgtgata
      121 tacctctatt acatcatcat tatcttccag ggcatctata agttttgcaa acttttccct
      181 gtcctcttca ttttcaagct tcacgtagga ctggggaacc attgtaattg aagcttctgc
      241 aaatttgtat ccctttttct ccaaaacatc ccttacactt gaaaaggaag aaggagcagt
      301 ggcaatctca taagcaccat caatatttga aaaatcttca gcttccgttt caagcacatc
      361 catcatcaat gtatcttctt caaaacttcc atcgtcttca ataacaataa taccctttct
      421 atcaaacata aaggacacac agttggttgc tccaagattt ccaccatatt tgtcgaaata
      481 gtgtctcatt tcaccagctg ttctgttacg gttgtctgta acagcctcaa ctatgactgc
      541 aactcctgaa ggaccgtaac cttcataggt aatttcctcc atgttgtcac tcatgccctc
      601 gcctgaagcc cttttaatac ttctttgtat gttatcatag ggcatgttgt tggccttggc
      661 ttttgcaata acatccttga gtcttgagtt tgtttcaggg tccggaccgc cttcgcgtac
      721 ggcaacctga atctccctgc caagctttgt aaatattttt gctctctgag cgtcagtttt
      781 gctcttttta tgctttatat tagcccactt agaatgacca gccataaaat tcctccaaat
      841 tatataaatt tatcacatca attatattcg aatttcacca aaccttcaag taacagggta
      901 atattccttt tcccctaaac ttaaataatt cttgccattc gtaacctcat tgacaatgtt
      961 gaccaccttg tcaacttcat cctcctccac aatatatgta acagtgacat tttcaccaaa
     1021 cacgggagct tgcgccattt tgtccgcaag agcgttctgc agctttccat aaaaagtata
     1081 ctccactgtg acactaacag gctgacacag aaccattctg caaagtcctg cgtcggcaac
     1141 cgccaatgaa gcagcttttc catatgccct tacaagacct gctgcaccaa gcaagacccc
     1201 tccaaagtat ctggtaacta caatcaccgc attcgtgata tcctgttttc gcaaaacatc
     1261 caatacagga ataccaccag ttccctgcgg ctcgccatcg tcagaatatc tggaaacgac
     1321 ttgtccgtcc tcatttacaa tgtacgcata gacattgtgc ctcgctcctt tgttcatacc
     1381 cttaatcttt tcaatataat caatgcactc ctgttcagtt gaaacaggct ttgcatatcc
     1441 tatgaactcg gaccgttttt caacatatgt tccgctgccc tcttttgata aagtaatata
     1501 tgacctgctc atacattccc tctttcaaat tctcgtattt tatgcatgta ctggtttgga
     1561 agagtgcctg taacttttat cccatcctcc gtatactgtt catctttcac cactcccttt
     1621 tcatgcaaaa aaggaagcac ccaagcctct ttgtaaggca ccaatatatc aaattcttca
     1681 aaaaaccttg taaagaagtc aaaaataccc tttttaagtt catcaaggcc ttctccagtc
     1741 ctggcagaaa cgtaaaaagt cttggcaaac accctgccag gctttacaac acctccatca
     1801 ctgacgatat ccatcttgtt caccacaaga tacttgggtt tgtttgcagc accgatgtcc
     1861 tttaatatag actctaccac ttcaattctt tcatccagat caggacttgt ggcatccacc
     1921 acatgaagaa gcaaatccgc ctgggttgcc tcttccaatg tagacttgaa ggcctccaca
     1981 agatgagttg gcagcttttt aataaagcca acagtatcaa caagaagata ggtgctgttg
     2041 tcttcggtaa tcattttcct aactgttgaa tccaaagtcg caaacagctt gtcctctaca
     2101 aaaacccccg cctcacaaag gcagttcata agagacgact ttcctgcatt cgtgtaaccc
     2161 accaatgaaa taacaggaac ttcatttttt attctttctt tcctcagaac ttccctttta
     2221 gatgctatct ccttcaatct gcgctgcaga tagttaattt ttctgtttat cgcccgcctg
     2281 tcggtttcaa gcttggtctc accggggcct cttgttccga taccgcctcc gagtcttgac
     2341 agaatctgac ccattcccgt aagtcttggc agcctgtatt gaagctgcgc cagttccacc
     2401 tgcaatttac cttccaggct tgttgccctc ttggcaaaaa tgtcaagaat gagagcggtt
     2461 ctgtcaatta tctttacacc ggtaatctcc tccaaatttc ttatctgggc gtagctgagt
     2521 tcatcgtcga atattaccaa atcagcattc aacgattgcc tgtgaaaggc cagctcctcc
     2581 gccaaacctt taccgatgta atatcttgca tcaatggatg ctcttttctg gacaaacctg
     2641 cccactacca aagctcccgc cgttcttgcc agctcggcaa gctcatcaag gtaagcttcg
     2701 tcctctcttg tggatacccc caccaatatg gctctttcct gctcttcctg agtcacttcc
     2761 cctattgtat aagttataac gtctgtttcc tcaacaattt caaacaactc atcaaacttg
     2821 tccagctctt tcatgctgta tggaccgtac aacttcgtat ttgaaagcct ctcttcctca
     2881 tctctttcca gcattgtgac ggatacaccc gtaacatgag gcttgtcatt gtaattaaca
     2941 acgccaatga caatcatgcc gtccagtctt agagtcttga gggaattcaa gtccacatcc
     3001 gaaggcaggc ttgaaccacc gggatgggtg tgtaaaaacc ttatgccgga caacctgttt
     3061 gttccccttc tgttatccat aaatggaagg gaaacagacc tgtcgtcccc tattacgatg
     3121 gcttcaaccc tgtttcttct attaatata
//
LOCUS       DAONVY010000262         3146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21179_length_3146_cov_2.845034, whole genome shotgun sequence.
ACCESSION   DAONVY010000262 DAONVY010000000
VERSION     DAONVY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21179_length_3146_cov_2.845034"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1219
                     /locus_tag="PLI11_07335"
     CDS             92..1219
                     /locus_tag="PLI11_07335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ52715.1"
                     /translation="MTTNVRMANKQDKEGIKKLWSEVFVDDSTNFVEWFYENRYNRAF
                     ATCLEADGEIASAMQGYPFNLRIRGRIVQASILAGVSTYEKHRGKGFMSKVFKAHMEQ
                     IRDAGIPLVPHTPANTPTFFSKGHYPVTETKIVTIKAMDYDSEAKIFPLKDIKSEYGN
                     MFACYTKWSLPYSGIVARSMSDFTLKMADYSADDSKVSILYKGSNVKAYGVFYILPEY
                     IYVEEIAYDSSESLTEMLKKVATLGKEVRAKLPTDSVVNIEGATSKTRLQGVVGVANM
                     SAFLKALFPFIPKDYEDSCIEVVDRIVDTNNGVYKLDGRVVNEKPHITIEAGRLAQVL
                     CGYKTLDEQLEENNVVVHDKAIFDRLNNLFPKEKCFIIDEY"
     gene            complement(1203..2426)
                     /locus_tag="PLI11_07340"
     CDS             complement(1203..2426)
                     /locus_tag="PLI11_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPZ52716.1"
                     /translation="MGKKRIALITVAISFSYAAGYVAKTVLSGIMPLIVEVGVFTKKD
                     LGAMGSAFMLAYGFGQLVNGIIGDILNPKRLIMAGLIGGGLVTFFFPYVQYASLAIFL
                     WGLCGFFCSTLWGPLSKIVAENMETRIARILMLFLTAASITGSMLAYVVCAVVSNWET
                     AFFVSGIYMIATSVLWYLIITLLEKRKQISYKTKRDKLLEGQKGLGINARYLVNHAII
                     PVTIAIMLNSIIRNAASFWIPTYIKEAFEVKASFSSAIIIVLPIVNLVGTIISYKMLP
                     LFKDNEHTLSACLFGFSSIMFILILLLGNVMSIAITAMFLASAAMSGVCNLLFSVYVL
                     RFSKTGRTSGIAGSLDFAAYMMASLANTLIGFWVEKFGWSFVILTWCIATVAGIAASV
                     VSHRLVANKDFNIHQ"
     gene            complement(2427..>3146)
                     /locus_tag="PLI11_07345"
     CDS             complement(2427..>3146)
                     /locus_tag="PLI11_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPZ52717.1"
                     /translation="EAYKGALDYTKFASASVEYWEKLVGEYENIVRRVTVDPLQDGVL
                     DFIPYLVSKSISVTIGHTAADAETVNKAIELGADSITHIFNAMPPMHHRNPGPVGAAL
                     SNRKCKCELILDFLHVSPIAAKVVIEAKGPENVAIVTDSCEAAGMPDGDYELGGRLVH
                     VRNGEARLPEGNLASSTVFMDQELRNVIELGYSIENAETMLAKTPASLVSYEHDEDYV
                     IVDKELNVKEVIINGKTVYRV"
BASE COUNT         1002 a          675 c          596 g          873 t
ORIGIN      
        1 atacattttt ttcaatatag ttttacaaca ttttaatgga atttgtttga aaacatgttt
       61 tagtatataa tacggttgga ggagataact tatgacaaca aatgtaagaa tggctaataa
      121 acaagacaaa gaaggtatta agaaactttg gagcgaagta tttgtagatg acagcacaaa
      181 ctttgtagaa tggttttatg aaaacagata caacagggca tttgcaacat gtcttgaagc
      241 agacggtgaa atagcaagtg caatgcaggg gtatcctttt aacttgagaa tcagaggaag
      301 aattgtgcaa gcctctattc ttgctggagt ctcaacatac gaaaaacatc gcggaaaagg
      361 atttatgagc aaggtgttca aagcccatat ggagcaaatc agagatgctg gtattccact
      421 tgttccccat acacctgcaa atacaccaac ctttttttcg aaaggtcact atcctgtaac
      481 ggaaacgaaa atcgtaacca tcaaagcaat ggattacgat agtgaagcaa aaatatttcc
      541 attgaaggac ataaaaagcg aatatgggaa tatgtttgca tgctacacaa aatggtcgct
      601 tccctactca ggcattgtcg cccgttcaat gtcggatttc accctcaaaa tggccgacta
      661 ctctgcagac gattctaaag tatccatatt atacaaaggt agcaatgtaa aagcttacgg
      721 tgtattctat atactacccg aatacattta cgtagaggaa attgcatacg acagttcaga
      781 gtccctgaca gagatgctga aaaaagtggc aacattgggc aaggaagttc gtgcaaaact
      841 acctacagac agtgtcgtca atatcgaggg agctacctca aagacaagac ttcaaggtgt
      901 tgtgggagta gccaacatga gtgcattcct taaagcttta tttcccttca taccaaaaga
      961 ttatgaggat tcatgcatag aggttgtcga caggattgtt gatacaaaca acggtgtata
     1021 caagctggat ggaagggttg ttaacgaaaa gccacatatc accatcgagg ctggacgtct
     1081 tgcccaggtt ctatgcggct acaaaactct tgacgagcaa cttgaagaaa acaatgtagt
     1141 agtacatgac aaggctattt ttgacaggct gaataacctt tttccaaaag aaaagtgttt
     1201 tatcattgat gaatattaaa gtctttgttg gcaacaagcc tgtgtgagac aacgctagca
     1261 gcaatgcctg caactgttgc tatacaccag gttagtataa caaatgacca gccaaacttt
     1321 tcaacccaaa aaccaatcaa ggtatttgca agggaggcca tcatgtatgc ggcaaaatcc
     1381 aatgagcctg caatgcctga ggtcctccct gtttttgaga acctgagcac atatacggaa
     1441 aacaagagat tacacactcc gctcattgct gcacttgcaa gaaacatggc agttattgct
     1501 atggacataa cattgcctaa tagaagtatc agaataaaca tgatactgga aaaaccgaaa
     1561 aggcatgcac tcaatgtgtg ctcattgtct ttgaaaagcg gcagcatttt gtagcttatg
     1621 atggtgccaa caagatttac gattggcaaa acaattatta tcgccgacga aaaagaagcc
     1681 tttacctcga acgcttcttt gatgtatgta ggaatccaga aggaagcagc gttcctgatt
     1741 atgctgttca gcataatggc aatagttact ggtatgattg catgattgac aagatacctt
     1801 gcgtttatac caagaccctt ttgcccttcc aataacttat ctctttttgt tttatatgat
     1861 atctgtttcc tcttttccaa taaagttata atcaaatacc atagaacaga agttgcaatc
     1921 atataaatgc cggatacaaa gaaagcagtt tcccagttgc ttactactgc acatactacg
     1981 tatgcaagca tagaaccagt aatacttgca gctgtcagga aaagcatcag tattcttgca
     2041 atccttgttt ccatgttttc tgcaacaatc tttgacaaag gaccccacaa ggttgaacag
     2101 aaaaacccgc acaatcccca aagaaaaata gccaaagagg catattggac atatgggaag
     2161 aagaaagtaa caagtcctcc cccaatcaaa cctgccatta tcaacctctt tggattaagt
     2221 atatcgccaa ttattccatt aacaagctgt ccaaatccat aggccagcat gaatgctgaa
     2281 cccatggcac ccagatcttt cttagtaaaa acaccaactt caacaattaa aggcattatc
     2341 ccgctcaaaa cagtcttggc tacataaccc gctgcataac tgaacgagat tgcaacagta
     2401 attaatgcaa ttctcttttt gcccatctat actctataaa ccgtctttcc gtttataatc
     2461 acttctttta cattcagctc tttgtcaact ataacatagt cctcatcatg ctcataagat
     2521 acaagtgaag caggtgtttt tgcaagcatg gtttcagcat tttcaatact gtacccaagt
     2581 tcaataacat ttctaagttc ctgatccatg aatacagtac ttgaggcaag attgccttcg
     2641 ggaagtcttg cttctccatt ccttacatga accagtctgc cacccagttc gtagtctcca
     2701 tctggcatgc ctgccgcctc acatgaatca gtaacaatag ccacattttc aggtcctttt
     2761 gcttcaataa ctacctttgc tgctattggc gatacatgaa gaaaatcaag tataagttcg
     2821 catttgcatt tcctgttcga aagagcagct cccacgggtc caggatttct gtggtgcatt
     2881 ggtggcattg cgttaaaaat atgggtaata ctatcggctc caagttcaat agccttgttt
     2941 actgtctctg cgtccgctgc tgtgtgccct atcgtaacgg atatgctttt tgacaccaga
     3001 tacggaataa aatccagaac tccatcctgc aatgggtcaa ccgttaccct tctgacgata
     3061 ttctcgtact cgcctactag tttctcccag tactccacac tggcagatgc aaattttgta
     3121 taatcaaggg ctcccttgta cgcctc
//
LOCUS       DAONVY010000263         3141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21213_length_3141_cov_3.079715, whole genome shotgun sequence.
ACCESSION   DAONVY010000263 DAONVY010000000
VERSION     DAONVY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21213_length_3141_cov_3.079715"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="PLI11_07350"
     CDS             <1..2047
                     /locus_tag="PLI11_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52718.1"
                     /translation="YTNSVVTQVFPDREQRPVDYSTWVATDDLGRVTPMQDETRAFQE
                     DKYVGLFYFLTGNAHRESQIVDVTKEYLARGIDGLKALLPTVPGIYWAEPYFGYYLNS
                     DTWVIRKHVQMFEAAGIDFIFLDVSNGETFDEAVPVLLDTLLQIRKEGGQTPEIAFMC
                     GDMPHILVHDLRIIYNLVYTKPEYEELFFKWEGKPLILGNNDIPGGEKWTVSHEAVGQ
                     YPEDRFWSSLSTEDREYFESGQYEQDLSNFTVRKSFAWQSRKYDNDKDYSGYWDFLDD
                     SPQAPGRSFKGDVEQVPVSIAILAHLGKGRSMTDNNPNYGNNKEDFEFTLGTAQYGYH
                     FAEQFERALEIDPKVIMITGWNEWVAGRQSNSFADMKTGHTTTPYYHFVDQFNPEFSR
                     DAEPMKLRDGVGYGDAYYYQMVSYIRKFKGMPPLPGSPWKNVVSFDVSISDGENRMQD
                     VDKKIMSQWDKVKTTYMDTINDIMFRNSPSYGLMTTYVNATGRNDIEYAKVTQDDKYM
                     YFLAKTVSGIITADEENWMNLFIDIDQNHETGWEGYDFVINRSRDGKTVSVEKFVNNG
                     WEFETVGTADYLMGSDNLTIRVSKELLGITGNEPEFDFKWADNSTITGNVMQFMDLGD
                     AAPDSRFNFRFTTKGVVPVDNERDEFPEMLIYVAIASAVVVAIVVVILIIVKKRKKA"
     gene            2068..2955
                     /locus_tag="PLI11_07355"
     CDS             2068..2955
                     /locus_tag="PLI11_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52719.1"
                     /translation="MKKKGIILLILITAFILLFVTACKKDETGKETPAPTATPAPTED
                     ANTPEPTATKEPGPTLKPISEEEKGKPHPEAEVLMKLDFSEVEEIDDIDYELRQPEHI
                     NIADGYMNIIGDWSAVSPNYGYGLGTDHNQYEWSATFHISHLSPDAPHVAYFVGARVP
                     QERTADYPGGIWLAFNYTRNVCVYIPGDNFLDENGEVIKNWASKYFTVTIPEDFTTEH
                     TVTVVDTGDEICYYMNTADDPQYLICRIVLDGEDLLIYNKDGETIKTFMNAVNEEINF
                     SVFSHRTKSHTKELVLKGY"
BASE COUNT          981 a          588 c          733 g          839 t
ORIGIN      
        1 gtataccaat tcggtggtta cacaggtttt ccctgatagg gagcagaggc ctgtcgacta
       61 cagtacatgg gttgcaacag acgaccttgg ccgcgtaact ccaatgcagg atgaaacaag
      121 agcattccag gaagacaagt atgtaggtct tttctacttc cttaccggaa acgctcacag
      181 ggaaagccag atagttgacg taacgaagga gtacctggca aggggaattg acggactgaa
      241 ggcgttgctt cccacggtac ccggaattta ctgggcagag ccttatttcg gttactatct
      301 gaattccgat acctgggtaa tcagaaagca tgttcagatg tttgaagctg caggcattga
      361 ctttatcttc cttgatgtgt ccaacggaga aacctttgac gaggctgtgc ccgtgttgct
      421 tgacacatta cttcagatta gaaaagaagg agggcagact cctgagatag ctttcatgtg
      481 cggtgacatg ccccatatac tggtacatga cttgcgcata atttacaact tggtatacac
      541 gaaacccgag tatgaggaat tgttcttcaa atgggaaggc aagcctttga ttttgggtaa
      601 caacgatatt ccaggtggag aaaagtggac tgtttcccat gaggccgttg gtcaatatcc
      661 tgaagacaga ttctggagct ccctttcaac ggaagacagg gaatactttg aaagtggtca
      721 atatgaacag gatttgagca actttactgt taggaagagt tttgcatggc agtccaggaa
      781 atatgacaat gacaaggact attcaggtta ctgggatttc cttgatgact ctccacaagc
      841 tcctggaaga agctttaaag gagatgtgga acaggttcct gtcagtattg caatccttgc
      901 gcatcttggc aagggaagaa gcatgactga caacaacccc aactatggaa ataacaagga
      961 agactttgaa tttacattgg gcacagccca atacggctat cactttgcag agcagtttga
     1021 aagagccctt gaaattgacc caaaggttat catgataact ggttggaatg aatgggttgc
     1081 cggaagacaa agcaactcct ttgcagatat gaagaccgga catacaacca cgccatatta
     1141 ccactttgtt gaccagttca acccagagtt ctcaagagat gcagaaccta tgaaattgag
     1201 ggatggcgtt ggctatggcg atgcatacta ctatcaaatg gtatcatata ttaggaagtt
     1261 taagggcatg ccgccacttc ccggatctcc ttggaagaat gttgtatcct ttgacgtatc
     1321 tatatccgat ggcgaaaaca gaatgcagga tgtggacaaa aagataatga gtcagtggga
     1381 caaggtaaaa accacttata tggatacaat aaatgatatc atgtttagaa acagcccttc
     1441 ctatggactg atgaccactt acgtaaatgc aactggaaga aacgatattg agtatgccaa
     1501 ggtaactcag gacgacaaat acatgtattt cctggcgaag actgtgtccg gaattataac
     1561 tgcggatgaa gagaactgga tgaatctgtt tatagacatt gatcagaacc atgaaactgg
     1621 gtgggaaggt tatgactttg taataaacag aagccgcgat ggaaaaactg tttctgttga
     1681 gaagtttgta aataacggct gggagtttga gaccgttggc actgcagact accttatggg
     1741 ttctgataac ttgacaatta gggtatccaa ggaactttta ggtataacag gaaacgagcc
     1801 tgagtttgat ttcaagtggg ctgacaattc gacaatcact ggcaatgtaa tgcagtttat
     1861 ggatttgggc gatgctgccc ctgacagccg tttcaacttc agatttacca ctaaaggagt
     1921 tgtgcctgtt gacaatgaaa gggatgaatt ccctgaaatg ctgatatatg tagctattgc
     1981 atcagcagtt gtagttgcaa tagttgtggt catattgata atagttaaaa aaaggaagaa
     2041 agcataaatg aaaggagaaa aaacaacatg aagaaaaagg gaattatctt actaatcctt
     2101 attaccgctt ttatactgtt atttgtaaca gcttgcaaga aggatgagac cggaaaggaa
     2161 acacctgctc caacagcaac acctgctccg acagaggatg ccaatacacc tgagcctact
     2221 gcaacaaagg agccgggtcc aacattgaaa cctatttctg aagaggaaaa gggcaaacct
     2281 catcctgaag cagaagttct tatgaaactt gatttctcgg aagtagaaga gattgacgat
     2341 attgattacg aattgagaca gcctgagcac atcaatatag ctgatggcta catgaatata
     2401 attggggatt ggtctgctgt ttcaccaaac tacggctatg gacttggtac cgatcacaat
     2461 cagtatgagt ggtcagctac attccatatc tcacatcttt cgcctgacgc tcctcacgtt
     2521 gcatactttg taggtgcaag agttccacag gaaagaacag ctgactatcc aggcggtatt
     2581 tggcttgcat tcaactatac aaggaatgta tgcgtgtata taccaggcga caacttcctt
     2641 gatgaaaatg gagaggttat taagaactgg gcttcgaagt atttcaccgt tacaattcct
     2701 gaggatttta ctacagaaca tacagtaact gtagtggata caggcgatga aatttgctac
     2761 tacatgaata ctgcggatga tccacagtat cttatctgca gaatagttct tgatggtgaa
     2821 gatttgctaa tctacaacaa ggatggagaa accataaaga ctttcatgaa tgctgtaaat
     2881 gaagaaataa acttctcggt tttcagccac agaaccaagt cccatacaaa ggagcttgta
     2941 ctaaagggtt attaatacta accacatcac actccctgca attgcgaaat gcaactgcag
     3001 ggattttttt atgctcaaaa gaattatgcg ttgatttgtc aaaagtaatt taggatatat
     3061 ctaagtcgtc agatgttgat ggaggtgtaa aatgataaaa agatttttgt tttgtgctct
     3121 aattgttgtt tttgtttttg t
//
LOCUS       DAONVY010000264         3138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21239_length_3138_cov_2.908855, whole genome shotgun sequence.
ACCESSION   DAONVY010000264 DAONVY010000000
VERSION     DAONVY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21239_length_3138_cov_2.908855"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLI11_07360"
     CDS             <1..416
                     /locus_tag="PLI11_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPZ52720.1"
                     /translation="NPTALLVATAAAEAVRMIGMPEARIILAHAAITVAASPKSNAAY
                     MAINKAMDDVSKKDTGEVPYYLRNPVAEGLKDIGYGEGYKYAHDYEGNVADLEFLPDI
                     MRGTIYYKPTFNGYEKRVSDYLKWVDEMKKRNRDK"
     gene            complement(453..1247)
                     /locus_tag="PLI11_07365"
     CDS             complement(453..1247)
                     /locus_tag="PLI11_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPZ52721.1"
                     /translation="MILDCKVIEDLLPLYLDNVCSDTSKQLVGEHLKECEDCRRLINT
                     TQVVGVPHFEPERPAVDNAVRKGLKRIRFRWWASILIVIIIVPMVFLGWNQYHGRGVH
                     FTNIYELQIGNAFMKYLDEGNYEKAYSYIDIAGLKQEWLKRWFDEEKLKNIEADGLAK
                     FCELGAKLEEHGGIQGYEYVGISHYGHDNDGTPIYQMIFKVNYAGKETLFDIMVSNDG
                     IEYFSGNGSFKTDPLAQFAIWSEYLWQDYEGCYYDPDLNEYVYPNK"
     gene            complement(1244..1729)
                     /locus_tag="PLI11_07370"
     CDS             complement(1244..1729)
                     /locus_tag="PLI11_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPZ52722.1"
                     /translation="MATFQKVYEEYAQPVYRFLLSLTGNDDLAEELLSETFYQAFRYI
                     DRFEGRCNLYTWLCQIGKNAWKKECRRNKRYSDIRLEDLTIPDNKPSLEEQNINKEMY
                     CKVLKALQDLADPYRDVFILHAIGEVKLKEIASIYGKSESWARVTYFRAKQQIAQEVL
                     K"
     gene            complement(1733..2512)
                     /locus_tag="PLI11_07375"
     CDS             complement(1733..2512)
                     /locus_tag="PLI11_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52723.1"
                     /translation="MPMFCFLSGLFLNNAKVCKKQLARIFPLYILLQTFAVVIGNGEV
                     NPLTPYWHLWYLLSYSIWLCLAWTWFRFCNGKGKIIILICSIVVGCVAGLVPFIGREL
                     SLSRTLVFLPYFWTGLICNPKFPWKKLRLAGIAALVTAFALIVCIGNDIPVKFLYQAT
                     PYEHIGKGVLLRLVCYLLGGSLGLFLLAFIPTRRLPFSKAGANTMPAFFYSRTDCIIL
                     TGIEHSVAVIHNIYCCFSICNIFACAVAQQLIRNRTYREEG"
     gene            complement(2781..>3138)
                     /locus_tag="PLI11_07380"
     CDS             complement(2781..>3138)
                     /locus_tag="PLI11_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255026.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPZ52724.1"
                     /translation="PTFPEVSDKTNATYLGKGISIAKYTGARGKSGASDANPEFVAEF
                     TSILNKENIPWQIGEMGKIDLGGGGTIAQYMADIGIEVLDCGPGVLSMHSPFEITSKA
                     DIYGCYKAYKCFYENI"
BASE COUNT          971 a          687 c          555 g          925 t
ORIGIN      
        1 caaatcctac tgcgctgctg gtcgcaaccg ctgcagctga agcagttcgc atgattggca
       61 tgcctgaagc aagaattatt cttgcacatg ctgcaattac tgtggctgca agtcccaagt
      121 caaatgcggc atatatggcg ataaacaagg caatggatga tgtttctaaa aaagatactg
      181 gagaggttcc atattatctt agaaatcctg ttgctgaagg attgaaggat attggatatg
      241 gtgaaggcta caaatacgcc catgattacg aaggcaatgt ggcagatttg gaattccttc
      301 ccgatattat gagaggaaca atatactata agcctacgtt taacggctat gaaaaaagag
      361 tttctgacta cctgaaatgg gtagatgaaa tgaaaaagag aaacagagat aaataaaaaa
      421 gcaaacccaa tggatttgcc cttgattaac aactatttgt ttgggtaaac atactcattt
      481 aaatcaggat catagtaaca tccttcataa tcctgccaga gatattcact ccatatagca
      541 aattgtgcaa gcggatctgt tttgaaactt ccattgccgc tgaaatattc gatgccatca
      601 ttggaaacca taatatcaaa caaggtttct ttgcctgcat agttgacttt aaatatcatc
      661 tgatagatag gcgtaccgtc gttatcatga ccgtaatggg atattcccac atactcatag
      721 ccctgaatac cgccgtgttc ctccagtttg gctcctaatt cacagaattt tgcaaggccg
      781 tccgcctcaa tattttttag cttttcttca tcaaaccaac gtttcaacca ttcctgcttc
      841 aaacctgcga tatcaatata gctatatgct ttctcatagt tgccttcatc cagatatttc
      901 ataaaggcat taccgatttg cagttcataa atattggtaa aatgaactcc acgtccatga
      961 tactggttcc aaccgagaaa taccattggt acaatgataa tcacgatcag aatggatgcc
     1021 caccagcgaa aacgaattct ttttaaaccc tttcttactg cattatctac tgctggtctt
     1081 tccggttcga agtgcggaac accaaccacc tgtgtggtat ttatcagcct tctacaatct
     1141 tcacattctt ttaaatgctc tcctaccagt tgtttgcttg tgtcagaaca tacattgtcc
     1201 aaatatagag gcagcaaatc ctctatgacc ttacaatcca atatcatttt aaaacctcct
     1261 gtgcaatctg ttgttttgct ctgaaatatg taacccttgc ccaactttcg ctttttccgt
     1321 aaatactggc gatttccttc aatttgacct caccgatagc gtgaaggata aaaacatccc
     1381 tgtatggatc agccaagtct tgaagtgcct tgagaacttt gcagtacatt tccttgttga
     1441 tattctgttc ttccaatgaa ggtttgttat caggaattgt caaatcttcc aatctgatat
     1501 cgctgtatct tttgtttcta cgacactctt ttttccaagc gttttttcca atttgacaca
     1561 accatgtata taggttgcat cttccttcaa atctgtcaat atacctaaac gcttggtaaa
     1621 aggtttctga cagtagttcc tctgccaagt catcgttgcc tgtcagggat aataggaatc
     1681 gataaacggg ctgagcgtat tcttcgtaga ctttttgaaa ggtagccacc tactatccct
     1741 cctctctgta ggtacgattc cgtataagct gctgtgccac cgcacaagca aatatattgc
     1801 atatagaaaa acaacagtaa atattatgta taacggccac tgaatgttca attcccgtaa
     1861 gtataataca atcggtgcgt gaataaaaaa aagctggcat tgtattagca ccggcttttg
     1921 aaaacggtaa tcttcttgtc ggtataaatg caagcaggaa taaccccagt gatccaccaa
     1981 gtagataaca aacaagccta agcaacacac cttttccaat atgctcgtat ggagtagctt
     2041 gatagaggaa tttaaccggg atatcattac ctatgcaaac tattagtgcg aaagcagtta
     2101 ccaatgctgc aattcctgcc aacctcaact ttttccaggg aaacttcgga ttgcatatca
     2161 gtcctgtcca aaaatacggc aggaacacta gagttctcga caatgaaagc tcacgcccga
     2221 tgaacggaac aagtcctgca acacaaccaa ccacaataga acatataagt attatgattt
     2281 ttcctttccc attacaaaat cgaaaccaag tccacgccag gcagagccat atactgtagc
     2341 tcaataagta ccacaaatgc caatatggtg tcaacggatt aacctcacca ttaccaatta
     2401 ccacagcaaa cgtttgcagc aggatatata atgggaatat ccttgctaat tgtttcttgc
     2461 agacttttgc attattcaaa aatagccctg aaaggaagca gaacattggc atatggacca
     2521 aatacatcca actgtattgc accataagca cttcagatta ccaaatcatt gactcgatta
     2581 aatggccata tatcacaaga aaaattagaa gaagttttag attgcaatat tgtgcatctc
     2641 ttaattttac tatctctatc atcatatatt agagacacaa agagcaaaaa cgttacacaa
     2701 gaaattataa aatattgtga caaaaaaatt aagggagaca acatccttgt gtagtgtctc
     2761 cctttcatga gcgtttttat ttatatattt tcataaaaac atttgtaagc tttgtagcat
     2821 ccataaatat ctgccttaga agtaatctca aaaggagaat gcatggaaag aactccaggg
     2881 ccgcagtcaa gaacctctat tcctatgtca gccatatact gggctatagt accgcctcct
     2941 ccgagatcaa tcttgcccat ttcgccaatt tgccagggga tgttttcctt gttaagtatt
     3001 gatgtaaact cggcaacgaa ctcgggattt gcatcacttg cgccactctt tcctcgtgca
     3061 ccagtgtatt ttgcaatact gatacccttg cccagatagg ttgcattggt tttgtcggat
     3121 acctccggga aagttgga
//
LOCUS       DAONVY010000265         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21304_length_3131_cov_3.745449, whole genome shotgun sequence.
ACCESSION   DAONVY010000265 DAONVY010000000
VERSION     DAONVY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21304_length_3131_cov_3.745449"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLI11_07385"
     CDS             <1..218
                     /locus_tag="PLI11_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPZ52725.1"
                     /translation="LTGTVRNVIDFGAFVDIGVHQDGLVHISEICDKYIKHPSEVLGV
                     GDIIKVKVIGVDIGRKRISLSMKNIPQ"
     gene            227..2863
                     /locus_tag="PLI11_07390"
     CDS             227..2863
                     /locus_tag="PLI11_07390"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPZ52726.1"
                     /translation="MRFSTERLLSLTFVIVLVVIMLVSIRHDYDKKPPTGHLELTVGE
                     VCIFSLMDNKYIKYNLEKDTEYEIYLDMDAKTFTDTYIGLSIDKGNIQIDGKDTNNTL
                     VPISNSKPITIKSDSDANLTVSVFTYNKSINKEILYTSNFNFLSSINDFSNEKEDLIL
                     VLTGDIKTTETLIIKRPCEFFLNGFNFESTQDFIVDFESTKPFIIHMRDKKQIRVNAF
                     YANTPNSDITIDKIFFKDEPEYAVRAKTLNGKSIGTKTTVKSAEKFLQLLDDNHYPYL
                     YPDTTISVGNFILDNKAVESFYNTFKKEITVTKPVRLVINKGFEIKDVVLNIQTDEKG
                     TLVVNVDENANFSYHHISIDAPNCSLLWTNSAPKEDYVAKYMNVQTYQGKPVNSFLGG
                     NGKGRIIEFASNDSLGLEWVVKGNVIEAQIPFDTSDIQLADIEISFMTSEGTAEIDKK
                     YVKDNNLVDLHESPLIVVTDEKGKTRTYMVSVKRKANSLPIIYIYTDDYRTIDSKEEY
                     IPGSFMMDADHVDGNYTIPTTSMNIRGRGNSTWRLFDKKPYRIKFDEKVSLFGLAPNR
                     DWVLLANYADHSLLRNYVAMEMGKKLTNLEFTPSSYPVDLFVNGEYMGVYSIGEQIEV
                     AKSRVDIDEDYDDPDTGYLLEVGGTDNVLELGVDFFHCMLLKWVAIKSPDTRAMSKEH
                     FDFISQFTKDADNAIVRNLDYENYVDIDSVIDWVILHEFTCNLDSGFRRSCFLYKDKG
                     GKLRMGPIWDFDLAFGNFFRDNSYDTWFTCGGETLGSTWTTFLYQYPKFNEKYKTRWN
                     EVKDVLKDTALSAVTNGKNMVYKSAMYNFEKWNKLLGNKTSLQPEHIREMKTYDDHIN
                     YLLNFINTRFEWLDNAINNLPS"
     gene            2927..>3131
                     /locus_tag="PLI11_07395"
     CDS             2927..>3131
                     /locus_tag="PLI11_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52727.1"
                     /translation="MSFNNGYYFFEHSDKSVSGYIMFRKSNGSVRVQVLVEGLNVPAD
                     KGQVYLSNAQGDKLHLADVPLRKM"
BASE COUNT         1064 a          602 c          626 g          839 t
ORIGIN      
        1 ttctgaccgg aactgtaagg aatgtaatag actttggagc ctttgtagac attggcgttc
       61 accaggacgg tcttgtgcac atatctgaaa tctgcgataa gtacatcaaa catccctctg
      121 aagttctagg agtgggagat ataatcaaag tcaaggttat aggtgttgac attggcagaa
      181 aaagaatatc tctcagcatg aaaaacattc cacaataggt gatatcatga ggttttccac
      241 ggaaaggttg ctttctctga ctttcgtaat tgttttagtt gtaatcatgc ttgtgtccat
      301 caggcatgat tacgacaaaa aaccacccac aggtcatttg gaactgactg tgggagaggt
      361 ttgcatattt tcactcatgg ataataaata cataaaatat aatcttgaaa aggacactga
      421 atacgaaatc tatcttgaca tggatgcaaa aaccttcacg gatacataca taggcctttc
      481 tatagacaaa ggaaatatcc aaattgacgg aaaagacaca aacaatacct tggttccaat
      541 atcaaacagc aaaccaataa ccataaaatc agacagtgat gcaaacctta ccgtctccgt
      601 ctttacatac aacaaaagca taaacaagga aatactatat accagcaact ttaatttcct
      661 aagcagtatc aatgacttct ccaacgagaa agaggatttg attctggttc tgacaggaga
      721 tatcaaaacg actgaaacgt tgattattaa aagaccctgc gaattcttct tgaatggatt
      781 caactttgaa tccacacaag acttcattgt ggattttgaa tccaccaaac catttataat
      841 tcacatgaga gataaaaaac agatcagggt taatgccttc tatgccaata ctccaaacag
      901 cgacataact attgacaaga tctttttcaa ggatgagccc gaatacgcag taagagcaaa
      961 gaccttgaac ggcaaatcca tcggcacaaa aacaactgta aaaagtgctg aaaagttcct
     1021 tcagctttta gatgacaatc actaccctta tctttatccg gatacaacaa tttcagttgg
     1081 caattttatt ctcgataaca aggctgttga atccttttac aacactttca agaaggaaat
     1141 aacagttaca aagcctgtca ggctcgttat taataaaggg tttgaaataa aggatgtcgt
     1201 gttaaatatc caaacggatg aaaaaggcac cttggtagtt aatgtggatg aaaatgcaaa
     1261 cttcagctat caccatattt caatagatgc cccaaactgc tcccttttat ggacaaattc
     1321 cgctcctaaa gaagattatg tggcaaaata catgaatgtt caaacatatc agggaaagcc
     1381 tgtaaacagc tttcttggcg gcaatggaaa aggaagaatt atagaatttg ccagcaatga
     1441 ctctctgggt ctggagtggg tggtgaaggg aaatgtaatt gaagcacaaa tcccctttga
     1501 taccagcgac atacagcttg ccgatattga aataagtttt atgacaagtg aaggcacagc
     1561 tgaaatagat aagaaatatg taaaagataa taatttggta gatctgcatg aatccccctt
     1621 gattgtcgtt accgatgaaa aaggcaaaac aagaacttac atggtatcag taaagagaaa
     1681 agcaaactct cttccaataa tctacatata caccgatgac tatagaacaa tcgattcaaa
     1741 ggaagaatac ataccaggtt ccttcatgat ggatgcggac catgtagatg ggaactatac
     1801 aattccaact acaagtatga atattagggg gagaggcaat tccacctgga gactttttga
     1861 caagaaacca tatagaatca aatttgatga aaaggtatct ttgtttggcc ttgcacctaa
     1921 cagggactgg gtgctccttg caaactatgc agaccactcc ctgctaagaa attacgttgc
     1981 catggaaatg ggcaagaaac ttacaaacct tgaattcaca ccttcttcct accctgttga
     2041 cttgtttgtt aacggtgaat acatgggtgt gtattcaatt ggcgaacaga tagaagttgc
     2101 aaaaagccgt gttgatattg acgaggacta tgacgatcct gacaccggat atctccttga
     2161 ggtaggagga actgacaatg tccttgaact gggtgtcgac tttttccact gcatgcttct
     2221 taagtgggtc gccataaaga gtccggacac aagggcaatg tcaaaagaac actttgactt
     2281 catatcgcaa tttacaaagg atgcagacaa tgccatagtc agaaatctgg attatgaaaa
     2341 ctatgtagac attgactcgg taattgattg ggttattcta catgaattta cctgcaacct
     2401 cgattcagga tttagaagaa gctgttttct ttacaaggat aaaggcggaa aactccgcat
     2461 gggacccata tgggacttcg accttgcctt tggcaacttc ttcagagaca actcttatga
     2521 cacatggttt acctgcggtg gagaaacttt gggaagcacc tggactactt tcctttacca
     2581 gtatcctaag tttaacgaga aatacaaaac gagatggaac gaggttaagg atgtactaaa
     2641 agataccgcc ctttctgcag tcaccaacgg taaaaacatg gtttacaagt ctgcaatgta
     2701 caactttgaa aaatggaata agttgcttgg caacaagact tccctgcagc ctgagcatat
     2761 tagggagatg aaaacctatg acgaccatat caactatctc ctaaatttca taaatacccg
     2821 tttcgagtgg cttgacaacg ctataaacaa cctgccctcg tagaaaaagt aatatttgcg
     2881 aaagcttgtc atataattta ccataactat ttagtgaggg atatgtatga gctttaacaa
     2941 tggatattac tttttcgagc atagtgacaa aagcgtaagc gggtatatca tgttcagaaa
     3001 gtcaaacggc tctgtacgcg ttcaagtttt ggttgaagga cttaacgtac ctgctgacaa
     3061 aggacaagtg tatctatcaa atgcgcaggg tgacaagctg catttggcgg atgtgccttt
     3121 gagaaagatg a
//
LOCUS       DAONVY010000266         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21305_length_3131_cov_3.301365, whole genome shotgun sequence.
ACCESSION   DAONVY010000266 DAONVY010000000
VERSION     DAONVY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21305_length_3131_cov_3.301365"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLI11_07400"
     CDS             <1..229
                     /locus_tag="PLI11_07400"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPZ52728.1"
                     /translation="RQLTISANSGLLGNFIGMLTDSRSFTSYVRFDYFRRILCSVVSR
                     YVVGGEYPGGDTAIQLVKDVCYYNVKKYIGI"
     gene            complement(272..694)
                     /locus_tag="PLI11_07405"
     CDS             complement(272..694)
                     /locus_tag="PLI11_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52729.1"
                     /translation="MKRKIALILFILIGIFLLTSCGNTVSTSEAKKYTTDFLEAIVAN
                     EYEKAEELLHPDAKEDEDVIEDFVKQLTDNNIILSGDVTDLKTTSINVAAYTSEHKGS
                     SCKIGGTVKVGGVTYNFICLVVNNKAGKGIFAFNIEKD"
     gene            776..1351
                     /locus_tag="PLI11_07410"
     CDS             776..1351
                     /locus_tag="PLI11_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPZ52730.1"
                     /translation="MIDLHLYIEGGDKVVDQDINSFFNEIYDRTFKNTIRYITAKCSN
                     VDDIHEIVQEVYADVYSTLVNKGVGYIKNPEAFIAKVTKSKVYRHYSLLERIRGVAIS
                     NENGVDDYGVETLETYTIEEEVINKQMVDDVFEHLSKKTDDVKRIFYLFYYVDMTIEE
                     IANDLDISVSNVKNKLYRTVRELRTIYGKGE"
     gene            1461..2258
                     /locus_tag="PLI11_07415"
     CDS             1461..2258
                     /locus_tag="PLI11_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52731.1"
                     /translation="MKKKFTIAASIVLVFVIVFATYAYVDAKEYKEAVLFLNEIGIQE
                     GMLSRSETKKVYKDIKSGNFDEPVTNAVLTKVAENYGLNIENQKAEEIYASIRTYNML
                     TYTKKILSEDVLNIEKGMTYKEIIDLLGETKDIGTVDEHTLMYVVDGTKLLYLKFSDE
                     DDVCELSGKELLDTLVNAVQDQKDKNTFKATVISKGKNHVFLSCPTYELFDSIYLMID
                     DKTEIVFEDGTKATVDDIEGEVIATFTGGIRESYPPQSNAKKIVIIR"
     gene            2351..>3131
                     /locus_tag="PLI11_07420"
     CDS             2351..>3131
                     /locus_tag="PLI11_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HPZ52732.1"
                     /translation="MGWRFRRSIKIADGVRLNFGKSGVSLSAGTRGLRRTYHTSGRVT
                     TSIGIPGTGLSYVTTNSKRKYEKSGRNYSQLSNINAVNNYEYYINLIRSLHKEGLEPI
                     DWQSIAEEEEPFKKGEMGPLQNEAVLRYEEYKPGVLEKVFSSLLAKKKEKLKQHILWA
                     EGEDAKVYEEWENRARFANGIIECNEQVCTTLLDEIKPFSDIEAFCEVRRCNFRKNGI
                     EIDVIIKKDVVPDHSLSLTKTYKISKKDLTKTTYYDIMQDYV"
BASE COUNT         1070 a          467 c          666 g          928 t
ORIGIN      
        1 aagacaactt acaatatcag caaacagcgg tcttttgggt aacttcatag gaatgctgac
       61 agacagcaga agctttacct catacgtacg ctttgattac tttagacgta ttctttgttc
      121 tgtagtatcg cgatacgttg taggagggga atacccaggt ggagatacag caatccagct
      181 ggtcaaggat gtatgttatt acaatgtgaa gaaatatatt gggatataat taaaaatgac
      241 gtcagccata ttaaggctgg cgtcattttt gttaatcctt ttcgatattg aatgcaaata
      301 tgccttttcc ggccttgttg tttactacaa ggcatatgaa attgtatgta actccgccta
      361 ctttcactgt tcctcctatt ttgcatgatg atcccttgtg ttccgaagta taagcagcta
      421 catttatgct tgtagttttt aaatcggtta cgtccccaga aagaataatg ttattgtctg
      481 tcaactgttt tacgaaatct tcaataacat cttcatcttc ctttgcatca ggatgcagaa
      541 gctcttcagc cttttcgtat tcatttgcta caatagcttc aagaaaatca gtggtgtatt
      601 tttttgcttc acttgtgctg actgtatttc cgcaagaagt caaaagaaaa ataccaatga
      661 gaataaaaag aatcaacgct atttttcttt tcattaccaa aatcacctct ttttatatta
      721 tctagattaa ctatcctata aaaataaaat aaagtaaaga ctatgaaact tttttatgat
      781 agatttacac ttatatattg aaggcggtga taaagtggtc gaccaggaca taaacagctt
      841 ttttaatgaa atttatgaca ggacctttaa aaatacaata cgatatataa ctgcaaaatg
      901 ctccaatgtt gacgacattc atgagattgt ccaggaggtg tatgcggatg tttattctac
      961 gcttgtaaat aaaggtgtcg gatacataaa aaatcctgaa gcctttattg ccaaggtaac
     1021 caaatcaaaa gtatacaggc attactcatt gttggaaaga atcagaggag tggcaattag
     1081 taatgaaaat ggtgtggatg attatggtgt tgaaactctt gagacctata cgatagagga
     1141 agaggtaata aacaagcaga tggtggatga tgtgtttgaa catctatcaa aaaaaactga
     1201 cgatgtaaaa agaatattct atctattcta ttatgtcgat atgacaattg aggaaattgc
     1261 aaacgatctt gatattagcg tatcgaatgt aaaaaacaag ttatatcgta ctgtgaggga
     1321 gttaagaaca atatatggga agggtgagtg aggtgaaaga aaaggaattt cttaagaatg
     1381 taattgagaa caaagttatg caaaaagaaa gaattcgaga aagaataata aatcaagggc
     1441 ataagaagga ggtactgcca atgaaaaaga agtttaccat agctgcatct attgtacttg
     1501 tatttgtgat tgtttttgca acgtatgcgt atgttgatgc taaggagtac aaggaagctg
     1561 ttctttttct gaatgaaata ggtattcagg aaggaatgct gtcccgttct gaaacaaaga
     1621 aagtatacaa ggatataaaa tccggcaatt ttgatgagcc tgtaaccaat gcagttctta
     1681 caaaggttgc tgagaattat ggcttgaata ttgaaaatca aaaagctgaa gaaatctatg
     1741 catcaatacg tacctacaac atgcttacct atactaaaaa gattttgtct gaagatgtct
     1801 tgaatattga aaagggcatg acatacaagg agataattga tttgctgggt gagacaaagg
     1861 atattggtac tgttgatgag catactctca tgtatgtggt ggatggaaca aaacttctct
     1921 acctgaagtt ttctgatgaa gacgatgtgt gtgaattgtc aggaaaagaa cttttggata
     1981 ctcttgtaaa tgctgtacag gatcaaaaag acaaaaacac atttaaagcg acagtaatca
     2041 gcaaaggtaa aaaccatgtt tttctatcct gtcctaccta tgagcttttt gattctattt
     2101 accttatgat tgatgataag acagagattg tgtttgagga tggtacaaaa gctacagtgg
     2161 acgatattga aggagaagtt attgcaacct ttactggagg catacgtgaa agctatcctc
     2221 ctcagtcaaa tgcaaagaag atagttataa ttagataggg aagagagtat caaggcagga
     2281 tttcctgcct tgatgcattt tcggccatta tgtggtatga tatatataat aatggttgga
     2341 ggtaaatata ttgggttggc gttttagacg aagtattaaa attgctgatg gtgtacggct
     2401 aaactttgga aagagcggtg tcagcctaag tgcaggcact agaggtttaa gacgcacata
     2461 tcacactagt ggcagggtta caacatctat tggcatacct ggaactggac tttcttatgt
     2521 cacaacaaac agtaagagaa aatatgaaaa gtcaggcagg aattattcgc aactcagcaa
     2581 tatcaatgct gtaaacaact atgaatatta catcaacctt ataagatccc ttcacaaaga
     2641 aggcctggaa ccaattgact ggcaatccat agctgaggaa gaggagcctt ttaaaaaggg
     2701 ggaaatggga cctttgcaga atgaggcggt tttacgctat gaggaatata aaccgggagt
     2761 tcttgaaaag gttttctcat ctttattggc taagaaaaag gagaaattga aacaacatat
     2821 attgtgggcg gagggggagg atgcaaaggt atacgaggag tgggagaaca gggcgaggtt
     2881 tgccaatggt attattgagt gtaatgagca agtttgtaca accttgttgg atgagattaa
     2941 accgttttca gatattgaag ctttctgtga agtaagaagg tgtaacttta gaaaaaatgg
     3001 tatagaaatt gatgtcataa tcaagaagga tgttgtccca gaccattctc tttctctaac
     3061 caaaacgtac aaaatctcaa aaaaggattt aaccaaaacc acatattacg acattatgca
     3121 ggactatgtt t
//
LOCUS       DAONVY010000267         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21307_length_3131_cov_2.646944, whole genome shotgun sequence.
ACCESSION   DAONVY010000267 DAONVY010000000
VERSION     DAONVY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21307_length_3131_cov_2.646944"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLI11_07425"
     CDS             <1..1098
                     /locus_tag="PLI11_07425"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPZ52733.1"
                     /translation="FYIIFESGGKMSDNVHMKPKLKPKTIILRVVIAFFSVLLVIVLG
                     LVGALSIIHYGPSEAARDLLVLSAMETSAGHFLATWFLPKEEIDRIIQDNSVVPTDVA
                     TNPDLIKIPDWNDDNDDDDNDEDNPKFDKNKIEIVDVSGKTYKGKMMIVYDPSRVYVG
                     TPTAYGKDKEGVRVIDMAIRDNAIGAVNGGGFEDTNGRGNGGIPIGIVISKGKLMWGE
                     MDKYYEVIGFDNNNILNVGYMTPRQAMDKGIRDAVTFGPILIVNGEPCEIKGTGGGYN
                     PRTAIGQRADGAVLLLVVDGRQANSLGATYTDLIDIMLDFGAVNAANLDGGSSSYMVY
                     ENEIITTRASLYQPRRMATAILVSRVDDEQE"
     gene            1102..2634
                     /locus_tag="PLI11_07430"
     CDS             1102..2634
                     /locus_tag="PLI11_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52734.1"
                     /translation="MRKIPLFYRILLIVIGVLVFFICVSLIYLWDWLKDYQEGLPVGR
                     MDELVKEIKAGDFNDFVSRYDFYITDFEDKENVINYLEENFKDVKKLTYKKMYSRTGE
                     RFSIYADEKAVLTVNLKKQKKGYTVSHLLLNTEIGIDIKVPKGFTVYLNDIEVSKKWV
                     DHEIGEEDASFENLFCMQDKDKVFEHYSILGLLRESHVRVCDYDGREVPLVLGDELRV
                     SYKKVTFLVPEDNAVYINDKKVDSSFITDTVESDVEGVTLNKYAVDGILNDFEYCVRD
                     EKDRDMLLEEENDTVCLKKIKYLIDAPQGFDVYVNGKKLDRKYLKETGVEVDELKYIP
                     EKYVEKPTYDRYEVAVLGIVPEVVVKNNDGFEVTTKTDNEKAVAGFQVPENVAQEYYP
                     IVEERAFMHAKFVANDLKMAQLSKVLKTDTQVYVVYKDLETQQWMYTDHIGYEFENVK
                     MGNLQMYNENFISCDISFDHIIIKTEKEVFTFPSNFTFYLAYLDNEWYIMDVIINSSA
                     SA"
     gene            complement(2612..3052)
                     /locus_tag="PLI11_07435"
     CDS             complement(2612..3052)
                     /locus_tag="PLI11_07435"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPZ52735.1"
                     /translation="MFFLYKFMSEALTEAYKCKQSGDIPVGCVIVKDGNIIARAHNTR
                     ERDKNPLGHAEINAIYNAAKHVGDWRLSGCDLYVTLEPCTMCAGAILSSRIENVFFGA
                     FDKKEGAAGSHTNLLYPNVNVYGGIMIEECVRLLDTFFKHLRSN"
BASE COUNT          980 a          526 c          746 g          879 t
ORIGIN      
        1 ttttatatta tttttgaaag tggtgggaaa atgtcggata acgtacatat gaaacccaaa
       61 ctgaagccca aaacaataat actaagagtt gttattgcct ttttttctgt attattggta
      121 attgttttgg gtcttgtggg agcattaagt ataattcact atggtccttc cgaagctgca
      181 cgcgatttgc tggttctttc cgcaatggaa acgagtgcag gtcattttct tgcaacatgg
      241 tttttgccaa aagaagaaat tgacagaatc attcaagata attccgttgt tccaactgac
      301 gttgccacaa atcctgactt aatcaaaatt cccgactgga atgatgacaa tgatgacgat
      361 gacaacgatg aagacaaccc caaatttgac aagaacaaaa tagagattgt tgacgtatcc
      421 ggtaaaacct acaaagggaa aatgatgatt gtatacgatc cttcaagagt ctatgtaggc
      481 acacctactg cctatgggaa agacaaggaa ggagtaagag tcattgacat ggccataagg
      541 gacaatgcta ttggtgctgt caatggtgga ggttttgagg atacaaacgg aagaggaaac
      601 ggcggcatcc ccattggaat agttatatca aaaggcaaac ttatgtgggg tgaaatggac
      661 aaatattacg aggtaatcgg ctttgacaac aacaacatat taaatgtagg atacatgaca
      721 cccagacagg caatggacaa gggaataagg gacgctgtaa ctttcggacc tattctcatt
      781 gttaatggcg agccttgtga gataaagggc accggcggtg gatacaaccc cagaactgca
      841 atcggacaaa gggcggatgg agctgttttg cttcttgtgg tggatggaag gcaggctaac
      901 agtcttgggg ctacatatac ggatttgatt gacataatgc tggattttgg agcggtgaat
      961 gccgccaatc tggacggagg gtcctcctca tatatggttt atgagaacga aatcataacc
     1021 acccgtgctt ccttgtacca gccaaggcgc atggcaacag ccatacttgt ctcaagagtg
     1081 gatgatgaac aggagtaaga aatgagaaag attcctttgt tttatagaat attattgata
     1141 gtaatcggtg tccttgtttt tttcatatgt gtctcgctga tctatttatg ggactggctc
     1201 aaggattatc aggaaggtct gccggttggc agaatggatg aactggtaaa agaaattaaa
     1261 gcgggtgatt ttaatgattt tgtcagccgc tatgatttct atatcactga tttcgaagat
     1321 aaggaaaacg tgataaatta ccttgaagag aatttcaagg atgtaaagaa gttgacttat
     1381 aaaaaaatgt actccagaac cggtgagagg ttctccatat atgcggatga gaaagcggtt
     1441 ttgacagtaa atttgaaaaa acaaaaaaag ggctacacgg tttctcattt gcttttgaat
     1501 acagagattg gaatagatat aaaggttcct aaaggattta cagtgtacct taatgacatt
     1561 gaggtatcaa aaaagtgggt ggaccacgag attggcgagg aagatgccag ttttgagaat
     1621 cttttctgca tgcaggacaa ggataaggtt tttgagcact atagcatatt gggtctgcta
     1681 agagaaagcc atgtaagggt ctgcgactat gacggcagag aagtgcctct tgttcttggg
     1741 gacgaacttc gtgtttcgta caagaaagtt actttccttg tacctgaaga caatgcggtt
     1801 tacataaatg acaagaaggt tgattcttct ttcataaccg acactgttga atccgatgtg
     1861 gagggagtaa cactcaacaa gtatgccgta gacggaatac tcaatgattt tgagtactgt
     1921 gtcagggatg agaaggacag ggacatgctt cttgaggaag agaacgatac tgtttgcctt
     1981 aagaagataa agtatttaat agatgctcct caaggttttg atgtttatgt taacggtaaa
     2041 aaacttgata ggaagtatct taaggaaact ggagtggaag tggacgagct taagtacatc
     2101 cctgaaaaat atgttgaaaa gcctacatat gacaggtatg aggttgcggt actcgggatt
     2161 gtccctgagg tggttgtaaa gaacaatgat ggatttgaag ttacgacaaa aacggataat
     2221 gaaaaagcag tggcgggatt ccaggtgccc gagaacgttg cacaagagta ctacccaata
     2281 gttgaggaaa gggctttcat gcacgcaaag tttgttgcca atgacctgaa gatggcgcag
     2341 ctgtcaaaag tgttgaaaac tgacacgcag gtttatgtgg tatacaagga tcttgaaaca
     2401 cagcagtgga tgtataccga ccatattggc tatgagttcg agaatgtgaa aatgggaaat
     2461 ctgcagatgt ataatgaaaa ttttatatcc tgcgatattt cctttgatca tataattatt
     2521 aagacggaaa aggaagtttt cacattcccg tccaatttta cattctatct ggcgtacttg
     2581 gataatgagt ggtacataat ggatgtgata attaattcga gcgcaagtgc ttaaagaagg
     2641 tgtccagaag gcgcacacat tcttcaatca ttattcctcc gtaaacattg acgttaggat
     2701 aaagaagatt ggtgtgcgag ccggcggcac cttctttttt atcaaaggca ccgaaaaaaa
     2761 cgttctcgat tctggaagag agaattgcac cggcgcacat tgtgcaaggc tcaagcgtta
     2821 catacaaatc gcagccggaa agccgccagt cacctacatg ttttgctgcg ttataaatgg
     2881 cgttgatttc tgcatggcca agtggatttt tgtccctttc tctggtatta tgtgctctgg
     2941 cgattatgtt tccatccttt acaattacac aacctacagg aatgtcgcct gattgtttgc
     3001 atttgtaagc ctcggtaaga gcttctgaca tgaatttata taaaaaaaac atattttatt
     3061 tacaaccttt cctgtgtttt gtggtatact tttaaaagtt acacctaaga aacaatcgat
     3121 taacaatata a
//
LOCUS       DAONVY010000268         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21341_length_3128_cov_2.974618, whole genome shotgun sequence.
ACCESSION   DAONVY010000268 DAONVY010000000
VERSION     DAONVY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21341_length_3128_cov_2.974618"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLI11_07440"
     CDS             complement(<1..623)
                     /locus_tag="PLI11_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ52736.1"
                     /translation="MSKIRVGIIGCGAIAHAAHIPAYLKCEDAEIVYFCDIIKERAEE
                     CVEKYGVGQAVTDYKVIAQDETIDAVSVCTPNNMHSIISIDMLRAGKHVLCEKPAART
                     YAEALEMQKVQQETNKVLNIGVVNRFNASVNEIRDLINSGKLGEVYHVYVSFRAFRSI
                     PGLGGDFTNKAVAGGGALIDWGVHYLDIVMYCLNDPKPETVSGQAYS"
     gene            794..1216
                     /locus_tag="PLI11_07445"
     CDS             794..1216
                     /locus_tag="PLI11_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPZ52737.1"
                     /translation="MRVFQFAKDMLIKYKRFIMFCMVGILNTAVTYVVYRILLYFSIN
                     FQIANAIGDIAGGVNSYLWNRFWVFKDSKVKTMESAPRFVVTFLVYMFASWLLMMLSV
                     DFIGIDERWAKLLVLPITTILNYFMNKVWTFAKRRHVE"
     gene            1372..2466
                     /locus_tag="PLI11_07450"
     CDS             1372..2466
                     /locus_tag="PLI11_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPZ52738.1"
                     /translation="MYVIGGLLIWLALKWDFEPALVMPMGFGAILVNIPFSGVVTQQM
                     EGLDEPVVGILDWLYEVGIGASEAMPILLFIGIGAMIDFGPLLSNPKMLFFGAAAQFG
                     IFLTLLVALLFGFDIKDAASIGIIGAADGPTSILVSQVLKSEYVGAIAVAAYSYMALV
                     PTIQPFAIKLVTTKKERMIRMPYNPKSVSRTTRILFPIFVTVIAGFVAPASIALVGML
                     MFGNLLRECMKLDNLSQTAQTSLANLITILLGLTIASTMKADKFVRIDTLIIMLLGLF
                     AFVFDTIGGVLFAKFMNLFLKDGKKINPMVGAAGISAFPMSARVIQKMALKEDNQNHL
                     LMHAVGANVAGQIASVVAGGIILSLVKQLL"
     gene            2497..2640
                     /locus_tag="PLI11_07455"
     CDS             2497..2640
                     /locus_tag="PLI11_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPZ52739.1"
                     /translation="MDWNLIKEGLLIMLKGMTGIFVVMLVIYLFIHLLSWITNRKANE
                     EKN"
     gene            2781..>3128
                     /locus_tag="PLI11_07460"
     CDS             2781..>3128
                     /locus_tag="PLI11_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52740.1"
                     /translation="MHRRTTIRSIVRIILFVNLLSATYIFTCSLQKQDNPLLTFLTVG
                     TIFAFLLVLLNPHFGRYTSDMQNVPTTLLLILILAFQVLARLFVLSYADNVDLVSLIT
                     LGIIAAGLISSLIA"
BASE COUNT          846 a          631 c          650 g         1001 t
ORIGIN      
        1 ttggaatatg cctgaccgct gacagtttcc ggtttcgggt cgttcagaca gtacatgact
       61 atgtccagat aatgcacgcc ccagtcaatc aatgctcctc cgcctgcaac tgccttgttt
      121 gtaaaatctc cgccaagacc tggaatgctt ctgaatgctc taaagcttac atagacatga
      181 tatacttctc cgagctttcc actgttgata aggtccctga tttcattaac acttgcattg
      241 aatctgttta caacacctat gttaaggacc ttgttggtct cttgctgcac cttttgcatt
      301 tccaaagctt ccgcataagt tcttgctgca ggcttttcgc aaagcacatg ctttcctgca
      361 cgcagcatgt caatggatat tatcgagtgc atgttgtttg gagtgcaaac agatactgca
      421 tcaatggttt catcctgtgc aataaccttg taatccgtta cagcttgacc aaccccgtat
      481 ttttcaacac attcttctgc tctttccttt attatgtcgc agaagtatac tatttctgca
      541 tcttcgcatt tcaaatatgc aggtatatgg gcagcatgcg caatagcgcc gcatccgatt
      601 atccccaccc tgatcttgct caaatagacc actcccttat cctagatatt cttacagaac
      661 accataattc taacctttct tgtatcttgt gtcaattata aatacttgtt tacctttatt
      721 ttcaatttag tggcaagata ttaatatttg ctttttcatg gtataataaa tgggagtaat
      781 tctggaggta tttatgagag tatttcagtt tgcaaaagac atgctaataa agtataagcg
      841 tttcattatg ttctgcatgg ttggcatact caacaccgct gtaacatatg ttgtataccg
      901 catattactg tattttagta ttaattttca gatagcaaat gccataggcg acattgctgg
      961 tggtgtgaac agctatttat ggaacaggtt ctgggttttt aaagactcaa aggtaaaaac
     1021 catggagtcg gcccccaggt ttgttgttac gtttttggtg tacatgtttg cctcttggct
     1081 tcttatgatg cttagtgtag attttatagg tattgacgaa agatgggcaa agcttttggt
     1141 gcttcccatt accacgattt taaactattt catgaacaag gtgtggacct ttgcaaagag
     1201 aagacatgta gagtaaaaat tcttatgcac aataaccata ttgtgatata atgttttgag
     1261 ggcatttttc atgttcccaa tttgctaaag gagagaagct gtttgagttt tttttcgaat
     1321 tttgagtttc ttattcatag tattaaaagt gtaacacctc agcaagtggt gatgtatgta
     1381 attggaggct tgctaatatg gctggcgctc aagtgggatt tcgagcccgc tttagtcatg
     1441 ccaatgggat ttggcgcaat tcttgtaaat attcccttca gcggcgtggt aacccagcag
     1501 atggaaggac ttgacgagcc ggtagtcggt attcttgact ggctatatga agtgggaatc
     1561 ggcgcttccg aagccatgcc cattctattg tttattggca taggtgcaat gattgacttt
     1621 ggaccactgc tttctaatcc aaagatgctg ttctttggag cggctgccca gtttggtata
     1681 ttcctgacac tgttggttgc acttctgttt gggtttgata taaaagatgc ggcatccata
     1741 ggcattatcg gcgcagctga tggacccacc tcaatacttg tatcacaggt tcttaagagc
     1801 gaatacgtag gtgcaattgc tgtggctgca tattcatata tggctctggt tccaacgatt
     1861 cagccttttg ctattaaact cgttaccacc aaaaaagagc gcatgataag aatgccatac
     1921 aacccaaaat cagtttccag aactacaagg attctgttcc ccatattcgt tactgtaatt
     1981 gcaggttttg ttgctccagc ctctattgct ttggttggaa tgcttatgtt tggcaacttg
     2041 ctacgtgaat gcatgaagtt ggacaacctc agccagacag cgcagacaag ccttgccaac
     2101 cttataacca tactacttgg ccttaccata gcatccacaa tgaaggcaga caagtttgta
     2161 agaatcgata cacttataat catgctgctc ggtctttttg cctttgtatt cgatacaata
     2221 ggcggagtgc tttttgccaa gttcatgaac ctgttcttga aagacggcaa gaaaatcaac
     2281 ccaatggttg gtgctgcagg tatatcggca ttcccaatgt cggctagagt tattcagaag
     2341 atggcattga aagaggacaa ccagaaccac ctgctcatgc atgcggttgg tgcaaacgtt
     2401 gcaggccaga ttgcatcggt ggttgcagga ggaatcatac tgtccttggt aaagcagcta
     2461 ttgtagacag taggctaatg tgaaagggat gatattatgg attggaattt gattaaagaa
     2521 ggcttgctaa taatgcttaa gggaatgact ggtatcttcg ttgtgatgct ggttatctac
     2581 ctgttcatac atcttttatc ctggataacg aacaggaaag caaacgaaga aaaaaattga
     2641 atcggaatca aggctgccag aaaacgggca gccttttcct ttaatattct tcttgttgat
     2701 attgggaata taatgtataa tcaaatgcga aggaaatata acaataatat acaaaagaaa
     2761 aagcatatac ggaggacacc atgcacagaa ggaccaccat tcggagcatt gtacgaatca
     2821 ttttgtttgt aaatctttta tctgctacat atatttttac ttgcagtctt caaaagcagg
     2881 acaacccact gctcactttc ttgaccgtcg gtacaatttt tgcttttttg ctagttttgt
     2941 tgaatcctca ctttggcaga tacaccagcg acatgcaaaa cgtacctaca acactgctac
     3001 tcattctgat actggcgttt caggttcttg ccaggttgtt tgtactttca tatgcagata
     3061 atgtagatct ggtctcatta ataacattag gtataattgc ggccggacta attagctctc
     3121 taattgcg
//
LOCUS       DAONVY010000269         3115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21462_length_3115_cov_3.278431, whole genome shotgun sequence.
ACCESSION   DAONVY010000269 DAONVY010000000
VERSION     DAONVY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21462_length_3115_cov_3.278431"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLI11_07465"
     CDS             complement(<1..626)
                     /locus_tag="PLI11_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPZ52741.1"
                     /translation="MKVYADLHIHTALSPCADEDMTPGNIVAMASLAGLDAIAITDHN
                     IALNVRPAIKYGEQFDVLVIPGMELETREEVHVVCLFETLEQVLDFQGVVHKSMTGLK
                     NNPDIFGRQLMFDEEDEIIGEYDDMLLCATDIGIDDVFSMIEGFGGIAYPAHVDRDSY
                     SVFSQLAFIPFDYPYKFVEISIDCKSDIIEKYPQLKKYNLLKASDSHD"
     gene            complement(623..946)
                     /locus_tag="PLI11_07470"
     CDS             complement(623..946)
                     /locus_tag="PLI11_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPZ52742.1"
                     /translation="MKVKTLVEELGMTLITGDGEKEITGIYACDLLSWVISHAKTGDL
                     WVTVMNNVNIVAVASLAEVACIVLAEGVKADEQVVKKARENGVTLLGSSMPTAEIIIK
                     SSKLI"
     gene            complement(943..2274)
                     /locus_tag="PLI11_07475"
     CDS             complement(943..2274)
                     /locus_tag="PLI11_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPZ52743.1"
                     /translation="MDDYYHSVVLDNKLCKGCTNCIKKCPMEAIRVRNGKAIIINEKC
                     IDCGECLRTCPYKAKIAQTDTWDDLSKFKYRIALPAPSLYMQFANAPSKEHVIKALQM
                     LGFDYVYEVARGAEIVTSITNKILKTESYQRPVISSACPAVVKLIQLNYPNLIPNLLK
                     IKSPMEMAAINAKKEFCEEHNVSPSEVGVFFISPCAAKMTAVKSPIGISKSNVDGVLS
                     IKTVYFRIFKQLAKIKDEELPDSNQAGYKGVRWAADGGEAVALNTEKILAVDGIHNVI
                     KIFEELENDSIEDVDFIEASACTGGCLGGPLAVENMFVATARTKKFKESLPSHGLSIA
                     DSEMEEYEFDCPIIHKDVTKLDTDISEAMRKYDRMVSLCDELPALDCGACGAPSCKAL
                     AEDIVRGIADITDCIFVLRRKVRKLAEEMMALESRMPPVIGEENNKKDGDI"
     gene            complement(2301..2735)
                     /locus_tag="PLI11_07480"
     CDS             complement(2301..2735)
                     /locus_tag="PLI11_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPZ52744.1"
                     /translation="MGLSGQKVTLRYTIAAEDFISAGEASSHVKKTLNKLGIRHELIK
                     RFTIAMYEAEINTVIHASGGEAYVEITPEKLMVKISDNGPGIPDIEKAMMEGWSTAPE
                     HIREMGFGAGMGLPNMKRYADDLAVESEVGVGTVVTITVYLS"
     gene            complement(2726..3079)
                     /locus_tag="PLI11_07485"
     CDS             complement(2726..3079)
                     /locus_tag="PLI11_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPZ52745.1"
                     /translation="MLVRDIVKTLDAEILTGEENLELDIQAGCGADLMSDVMAFVKDN
                     VILLTGLINVQVVRTAEMMDIKVVCFVRGKQPTEQIIELAKSKGITILSTKNPMFIAC
                     GLLYKAGLTGRGVWD"
BASE COUNT          844 a          785 c          525 g          961 t
ORIGIN      
        1 aaatcatgac tgtctgacgc ctttaataga ttatattttt ttagttgcgg atatttctca
       61 attatgtcgg atttgcaatc aatggatatt tcaacaaatt tgtaaggata atcaaacggt
      121 atgaaggcca gctgggaaaa cacgctgtag gaatcccggt ccacatgagc aggataagca
      181 atacccccaa aaccttctat cattgaaaaa acatcatcta tccctatatc cgtagcacat
      241 aggagcatgt cgtcatactc gccgattatc tcgtcttcct cgtcaaacat tagctgtctt
      301 ccaaatatat cgggattgtt ttttaatccc gtcattgact tatgcaccac gccctggaaa
      361 tcaagaacct gctccaatgt ttcgaaaaga catacaacgt gcacctcttc ccttgtttca
      421 agctccattc caggaataac aagcacatca aactgttcgc catactttat tgcaggacgc
      481 acattcaatg caatattgtg gtccgttatt gcaatcgcat caaggccggc aagagacgcc
      541 atggctacta tgttgccagg ggtcatgtct tcgtccgcac atggtgacaa ggcggtatga
      601 atatgcaaat ccgcatagac tttcatatca atttgcttga ttttataatt atctcagcag
      661 tcggcatgga tgaccccagc aatgtcacgc cattttccct ggcttttttc actacctgct
      721 catctgcctt aacaccttct gcaagaacaa tgcaagcaac ttcggcaaga gaagccactg
      781 caactatatt aacattgttc atgacagtca cccaaagatc tccggtcttt gcgtgagata
      841 taacccagct taagaggtcg catgcataaa tccctgttat ttctttttct ccatcgcctg
      901 ttatcaaagt cattcccagc tcttcaacca aagtcttaac cttcatatat ctccatcctt
      961 cttgttgttc tcctctccta taacaggagg catcctgctc tccaaagcca tcatttcttc
     1021 agcaagcttt cttaccttcc ttctaagaac gaatatgcag tcagtaatgt ctgcaatccc
     1081 cctcactata tcttcagcaa gagccttaca gcttggagca ccacaagctc cgcagtccag
     1141 tgcaggtaat tcatcacaca gacttaccat cctgtcatac tttctcattg cctctgatat
     1201 gtcggtatcc agttttgtaa catctttatg tataattggg cagtcaaact catactcttc
     1261 catttcagaa tcagctatgg acaatccatg agaaggcagg gattctttaa actttttggt
     1321 tcttgccgtt gcaacaaaca tattctccac agccagcgga cctcccagac atccacctgt
     1381 acatgcagag gcttctataa aatccacatc ttcaatactg tcattttcaa gttcctcaaa
     1441 tattttaata acattgtgaa taccatcaac ggcaagtatc ttttcagtat taagagcaac
     1501 agcctctcct ccgtcagcag cccaacggac acccttgtat ccagcctgat tactgtctgg
     1561 caattcctca tcttttatct tagccagctg cttgaatatc ctgaagtaaa ccgtctttat
     1621 tgacaataca ccgtccacat tggatttgct aataccaata ggagacttta cagctgtcat
     1681 tttagccgca cacggggata taaagaaaac tcccacctcg gaaggagaaa cattatgctc
     1741 ctcgcaaaat tctttttttg cattgatagc ggccatctcc attggcgact ttatcttaag
     1801 caggttgggt atcaaattag ggtaatttag ctgaatcagc ttaacaaccg caggacatgc
     1861 cgatgatatt acgggtcttt gatacgattc cgttttcaat attttattcg taattgatgt
     1921 tacaatttca gcgcctcttg ccacttcata cacataatca aatcccaaca tttgcagagc
     1981 cttgattaca tgttcctttg aaggagcatt ggcaaactgc atgtataaag aaggtgcagg
     2041 caaggcaatt ctatacttga atttagacaa gtcgtcccat gtgtctgttt gtgcaatctt
     2101 tgccttgtaa gggcatgttc tcagacactc tccacaatca atacactttt catttattat
     2161 tatggctttt ccatttctga cccgtatggc ttccattggg catttcttta tacaattggt
     2221 gcaacccttg cacaatttat tatcaagaac tacggagtga taatagtcat ccacattccc
     2281 caacctcctt ttcactcact tcaggacagg tatacggtaa tcgttacaac agtaccgaca
     2341 cctacttcac tttcaacagc aagatcatct gcatatcttt tcatgttagg caagcccatg
     2401 ccagctccaa atcccatttc tctaatatgt tccggggctg tggaccatcc ttccatcatg
     2461 gctttctcaa tatcaggaat tccggggcca ttgtcggata ttttaaccat caacttttct
     2521 ggagttattt caacgtatgc ttctccacca cttgcgtgga ttaccgtatt tatttccgcc
     2581 tcgtacatgg caattgtaaa gcgcttaata agttcatgtc ttattcccag cttgttgagc
     2641 gtctttttta catggctgga ggcttctcca gctgagataa aatcttctgc agcaatggtg
     2701 taacgcagtg taactttctg cccactcaat cccatacccc ccgtcccgtt agtcccgcct
     2761 tgtacaaaag accgcaggca ataaacatgg ggtttttagt agataatatg gtaatacctt
     2821 ttgatttggc aagttcaata atctgttccg ttggctgttt gcctcgtaca aaacaaacaa
     2881 cctttatatc catcatttca gccgttctaa caacctgcac gtttatgagg ccagtaagca
     2941 atatgacatt gtctttgaca aaagccatta catcgctcat caggtctgct ccgcaacctg
     3001 cctgtatgtc aagttcaaga ttttcctcac cagtcaaaat ttctgcatcc aaggttttta
     3061 caatatctct gacaagcata agttctcctc tcaatagtaa aatagggttg tagct
//
LOCUS       DAONVY010000270         3111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21493_length_3111_cov_4.009817, whole genome shotgun sequence.
ACCESSION   DAONVY010000270 DAONVY010000000
VERSION     DAONVY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21493_length_3111_cov_4.009817"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLI11_07490"
     CDS             complement(<1..469)
                     /locus_tag="PLI11_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52746.1"
                     /translation="MNKGRNIAILLGCMLCISILTFLVRWNIGNHQGVVDNVRYVKGE
                     TKDDFTEYEFYFIDRDKLDATLEYKGDNEWMIIFDDYEYNYRVVGTNRKDAKIEALPQ
                     YDVLETEPFDVISKRVFLYMIFGKPPVLTIPQAILVFLFFVSGLFVIIKAEELW"
     gene            complement(479..1726)
                     /locus_tag="PLI11_07495"
     CDS             complement(479..1726)
                     /locus_tag="PLI11_07495"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPZ52747.1"
                     /translation="MYLRILLGVDSLVDLVKMLKKYNQEHLLGFYDELDEDGKTNLIS
                     QIKGIDFELISELYEMAQKGSDEKTDPSDIQPMAYTDKDEISSVNKDRYKAIGRKICE
                     NGEYAVVTMAGGQGTRLGHTGPKGTFDIGIGKSLFEIQCERIRNMGFNVPWYIMTSKE
                     NDADTKAFFEANNYFGYDKENIIFFTQRMLPMMLENGRIVLEEKNRIKEGADGHGGIF
                     RAMIESGVLDDMKRRNIKWIFTCGIDNVLTRPDDLLFLGYVSEKGSMIGCKSLVKRGP
                     CEKVGVFCKVKGRPSVIEYTEISEEMANLRDEKGNLVYGDAHILLNLFNISVFEKMGG
                     RGLPYHVAFKKTDYINEKGEKVLADKPNAYKYEAFIFDAFSYFDDIAILRVKREEEFA
                     PVKNKEGEDSPKTALELYLNADK"
     gene            complement(1914..>3111)
                     /locus_tag="PLI11_07500"
     CDS             complement(1914..>3111)
                     /locus_tag="PLI11_07500"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPZ52748.1"
                     /translation="GKAFQDERTRNPRREIDGWFPRNYNQRGREWERPCRIQIFDREG
                     KLIIDQNVGVRIAGGMSRHNSIKSLRIISRSVYDEQHDSFAYEFFDAAYDVHGNKIKE
                     FERIILRNSANDFGQTMFKDAFLHKLGGMAGVDFQDSAPCVVYINGKYYSLMNMRESI
                     DADYIEAHYKIPKDHVTMVSIASGTGFTFSYKLTDGPEEGLDEFRKDMRKLISTDFSK
                     RDISEIEAIIDVDNFIKYMAYQMYIANNDWPHNNVLAWKYTGPTNNSVYGMDGKWRFI
                     LKDLDIQAAFTSPHHNSYEAVLNSGMSFNEPAIGQVFRSCVRNKEFASRFKEYMRKLC
                     TEILTKEAVNNLLDKFMYERMKDMQQFWGYYGGEMRSWESLINTYRDFVEPRNRFMLQ
                     HLNSYF"
BASE COUNT          828 a          770 c          505 g         1008 t
ORIGIN      
        1 accaaagctc ttccgctttg attatgacaa acaaaccgga aacaaagaac aagaacacaa
       61 gtatggcctg gggaattgtc aataccgggg gcttgccaaa tatcatatac aaaaagactc
      121 ttttgctgat aacatcaaaa ggttccgtct ccaaaacatc atattggggc aaagcctcaa
      181 tcttcgcatc ttttctattt gtaccgacaa ctctgtagtt gtactcataa tcatcgaaaa
      241 taatcatcca ctcgttgtcg cccttgtact ccagcgttgc gtccaacttg tccctatcta
      301 taaaataaaa ttcatactct gtaaaatcgt cttttgtctc ccccttgacg tatcttacat
      361 tgtcaaccac gccctgatgg ttgccaatgt tccatctgac aaggaaggtc aggatgctta
      421 tgcaaagcat gcatcccagc aaaatggcaa tattcctgcc tttattcaca caatcacctc
      481 atttatccgc attcagataa agctccagag cagtctttgg actgtcttcg ccttctttgt
      541 tctttacagg agcaaattcc tcttcccttt tgactctcaa tatggcaata tcatcaaagt
      601 atgaaaaggc atcgaatatg aaagcttcat acttgtatgc atttggcttg tccgctaaaa
      661 ccttttcgcc cttttcattt atataatcag tcttcttgaa agccacatga taaggaagcc
      721 ctctgccgcc cattttctca aataccgaga tattgaataa attcaataaa atgtgggcgt
      781 ctccatatac cagattccct ttttcatccc tcagattggc catttcctcc gatatctcag
      841 tgtattctat aacggaaggt cttcctttta ccttgcaaaa tacgccgacc ttttcacaag
      901 gtcctctttt tacaagagat ttgcatccta tcatgctgcc cttttcactt acataaccta
      961 ggaacaaaag atcatcgggt cttgtaagta cattgtcaat accacatgta aatatccact
     1021 ttatatttct tcttttcata tcatcaagaa caccggattc aatcatggct ctgaatatac
     1081 cgccatgacc gtcagctcct tccttgatgc ggttcttttc ctcaagaaca atcctgccgt
     1141 tttccagcat cataggcagc attctttgtg tgaagaatat tatattttcc ttgtcgtatc
     1201 caaaatagtt gtttgcttca aaaaatgctt ttgtatccgc atcattttcc ttgctggtca
     1261 taatatacca agggacattg aatcccatgt tcctaattct ttcacactgt atttcaaaca
     1321 gggattttcc aattccaatg tcaaaagtgc ctttgggtcc ggtatgacca agccttgtac
     1381 cctgcccgcc tgccattgta acaaccgcat attcaccatt ttcacagatt tttcttccta
     1441 ttgccttgta gcgatctttg ttcacgcttg atatttcatc tttgtcggta tatgccattg
     1501 gctggatgtc acttggatca gttttctcat cacttccttt ttgagccatc tcataaagct
     1561 cgcttattaa ctcaaaatca atccccttta tctgcgatat cagattcgtc ttgccatctt
     1621 catccagttc gtcataaaag ccaagcaaat gttcctgatt gtactttttt agcatcttaa
     1681 ccaaatccac caaactatca acccctaaaa gtattcttaa gtacatctaa tgcaatacca
     1741 catatatgat acctcttgtt ccacaaacga gctcttgcag aatgctgtca ttcatcccgt
     1801 gcatataatt atacagaaaa tagaagagct gtgcaaatgc tacagccctt atgaacaaaa
     1861 ctggcaatgc aacccaggag gattttgcag cctccagggt tgcatttttt cttctaaaaa
     1921 taactgttca aatgttgcag cataaatctg tttcttggct cgacaaaatc cctgtacgta
     1981 ttaatcaggc tttcccagct tctcatctct ccgccatagt atccccagaa ctgctgcata
     2041 tccttcattc gctcatacat gaatttgtca agaagattgt ttacagcttc cttggttaat
     2101 atttcagtgc acaatttcct catgtattcc ttgaatctgc ttgcaaactc cttgttcctt
     2161 acacagcttc tgaagacctg ccctattgca ggctcattga aactcattcc actattcaga
     2221 acagcttcat acgagttgtg atgcggagat gtaaatgccg cctggatgtc caggtctttc
     2281 agtatgaatc tccattttcc atccatgcca tagacactgt tgttcgtagg tcctgtgtac
     2341 ttccaggcaa gaacattgtt gtgcggccag tcgttgtttg ctatgtacat ttgatatgcc
     2401 atgtacttga taaaattgtc cacatcaatt attgcttcta tttcagaaat atccctcttg
     2461 gaaaagtctg tacttataag cttacgcata tcctttctga actcgtcaag gccttcttcc
     2521 ggaccatctg tcagcttgta tgaaaatgta aagccggtgc cggatgcaat acttaccatc
     2581 gtaacatgat ctttgggtat cttgtagtga gcttcgatat agtccgcatc tatggattca
     2641 cgcatgttca taagagaata gtattttcca tttatgtata ccacacaggg agcggaatcc
     2701 tggaaatcaa ctcccgccat gcctccaagc ttatgcaaaa aggcatcctt gaacatggtt
     2761 tgtccaaagt cattggcaga gtttctaagt ataatccttt caaactcttt tatcttgttt
     2821 ccatgcacat cataagcagc atcaaaaaac tcataagcaa aggagtcgtg ctgctcgtca
     2881 tacacgcttc tagaaataat tctcaaggat tttatcgagt tgtgcctgga catgccgcct
     2941 gcaattcgaa cccccacatt ttggtcaatt atcagcttcc cttccctgtc gaatatctga
     3001 atgcggcatg ggcgttccca ttcccttcct ctttggttgt agtttcttgg gaaccaacca
     3061 tcaatttcac gtctggggtt tctggtgcgt tcatcttgga aagcctttcc c
//
LOCUS       DAONVY010000271         3102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21573_length_3102_cov_3.306531, whole genome shotgun sequence.
ACCESSION   DAONVY010000271 DAONVY010000000
VERSION     DAONVY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21573_length_3102_cov_3.306531"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PLI11_07505"
     CDS             <1..1475
                     /locus_tag="PLI11_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPZ52749.1"
                     /translation="LTLVLVITFYIIRVFINDYDTYEIESVNFRSEDSISVYELQSVF
                     NNLMKMTKETGGNIKKSKYFSVISELLDKSGTLVEIKVDQMTTYRTEGYENDDIYRIA
                     CNVTDADVNNHTAVMYSDTSSFVYRTDLALDDGKVAEILLVNEKVGARELLKDNDKFQ
                     ADRIRRLKDSILSISFIGTIVIIILNMVIVAMVSNSITKSLYKLKEGARMIAEGNLDF
                     EIEDDTNDEVTEVIQTFDFMRSKLYESTMNQRRQEEHKKEMIAGISHDLRTPLTSIKG
                     YVAGLIDGIADSPEKQQQYLTTIYNTAEEMGNLVDELFLFSKLDLDRVAFNFEKTDIV
                     EYLSFCCDDLMFSLEKRNVVLTFTNLIKEKVYVNIDKNQFSRVLLNIADNTAKYKVNE
                     IGRLDISVEKEEDIVRIELKDDGVGVPAHAAEKIFDSFYRNDPARTNPVSGSGLGLAI
                     ARQIIEAHDGRIYARSNVGEGLTIIIEMNIIKSGNKEGIE"
     gene            1472..2164
                     /locus_tag="PLI11_07510"
     CDS             1472..2164
                     /locus_tag="PLI11_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPZ52750.1"
                     /translation="MKKVLIVEDDKNIASLERDYLEANGFETDIELTGESGLKKALEN
                     DYDLIILDIMLPGIDGFEICRILRRDKDTPILFVSAKKDDIDKIRGLGLGADDYIVKP
                     FSPSELVARVKAHINRYERLSNKQQDKSEVIEVGDLRIDVKARRVFKNEKEVILANKE
                     FDLLLFLAMNPNVVFSKDALFDKIWGFDSMGETSTVTVHINRLRDKIETDTQKPKYIE
                     TVWGAGYRFNLQ"
     gene            2229..>3102
                     /gene="hprK"
                     /locus_tag="PLI11_07515"
     CDS             2229..>3102
                     /gene="hprK"
                     /locus_tag="PLI11_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPZ52751.1"
                     /translation="MYKYSVKLEKIIEEFQLKVLCCEEQVKTIEIETKDINRPGLELA
                     GYTNHFSNDRIQLMGMVEYSYLKELSLQTRKKKIDKLFSEGFPCLIVARGLEVFPELI
                     QIANKYNIPVMSHHEATTELFSSLNRYLSVQLAPRQTLHAGLVEVYGEGVLIMGESGV
                     GKSETALELVKRGHRLVADDVVEVRRVSNKTLVGSAPETIRHLIEIRGLGFIDIRRLY
                     GIGSVKITENIKLVVKLENWQKGKEYDRVGITDQYTNILGIEVPSLTIPVRPGRNLAV
                     IVEVAAMNNRQKDFG"
BASE COUNT         1026 a          496 c          748 g          832 t
ORIGIN      
        1 tgctcacgct ggtgttggtt ataactttct atatcatacg tgtctttata aacgactatg
       61 acacgtatga gattgagagt gtcaatttca gatctgaaga cagtatttca gtgtacgagc
      121 ttcagtcggt ttttaacaac ttgatgaaaa tgaccaaaga aacaggagga aacatcaaga
      181 aatccaagta tttctccgtg attagtgaat tgctggacaa atcaggcact ttggtggaga
      241 taaaggtgga ccagatgacc acataccgca cggaaggtta cgaaaatgac gatatttaca
      301 gaattgcctg taacgtaacc gatgcagacg tcaataacca cacggctgtc atgtattcag
      361 acacatcaag tttcgtttac agaacggatt tggctttgga tgacggcaag gtggcggaaa
      421 tattgcttgt aaatgaaaag gtaggagcaa gggagctttt gaaggacaat gacaagtttc
      481 aggcagacag aataagaagg ttgaaggatt ccatcctttc catttcattt ataggtacca
      541 ttgtaataat tattcttaac atggttattg tcgccatggt gtcaaacagc attacgaagt
      601 ccttgtacaa actgaaggaa ggagcaagga tgattgcaga gggtaatttg gattttgaga
      661 ttgaggatga caccaacgat gaagtgacgg aagtcataca gacttttgac tttatgagaa
      721 gtaagctata tgagtcaaca atgaaccagc gcaggcagga agaacacaag aaagaaatga
      781 ttgctggaat ctcccacgac ttgcgcacac cgcttacttc catcaaggga tacgttgcag
      841 gacttattga tggaattgcc gacagtcctg aaaaacaaca gcagtatctt accacaatat
      901 acaatacggc agaagaaatg ggtaatctgg ttgatgaact attcctgttt tcaaagcttg
      961 atcttgacag ggttgctttt aacttcgaga agactgatat tgttgagtat ttgagttttt
     1021 gttgtgatga cctaatgttc agtttggaaa agcggaatgt ggtcctgaca tttacaaacc
     1081 ttataaaaga aaaggtgtat gtcaatattg acaagaatca gttcagcagg gttcttctaa
     1141 atattgcaga caataccgcc aagtacaagg ttaatgaaat aggaaggctt gacatatcgg
     1201 ttgaaaaaga agaagatatt gttagaatag aattgaagga tgacggagtg ggggtacccg
     1261 cccatgctgc ggaaaaaata tttgattctt tctatagaaa tgatcctgca agaacgaatc
     1321 ctgttagcgg aagtggtctt ggtcttgcaa tagctagaca aataattgag gcacatgatg
     1381 gaaggatata tgcaaggagt aatgttggag aaggacttac aataattatt gaaatgaaca
     1441 taattaaaag cggcaataag gaggggattg aatgaagaaa gttttgatag tggaagatga
     1501 taagaatatt gcatcgttgg aaagagatta tctggaagcc aacggatttg aaacggatat
     1561 tgagcttaca ggcgaaagtg gtttgaaaaa agctcttgaa aacgattatg atttgataat
     1621 actggatatc atgctgcctg gcattgacgg gtttgaaata tgcaggatac ttagaaggga
     1681 caaggatacg ccaattttgt ttgtatccgc taagaaggat gatattgaca agattagggg
     1741 gcttggcctt ggggcggatg actatattgt taagccattt agtcccagcg aattagtggc
     1801 aagagtaaaa gcccatatta acagatacga gcgcctcagc aacaagcagc aggacaagtc
     1861 tgaagttatt gaggttggag atctcagaat agatgtgaag gcaaggcgtg ttttcaagaa
     1921 tgagaaggaa gtaattttgg ccaacaagga gtttgacctt cttctgtttc ttgcaatgaa
     1981 tcccaatgtt gtgttttcaa aggatgcctt gtttgataaa atttggggat ttgactccat
     2041 gggcgaaact tcaaccgtga ctgtgcatat taacagactg agagacaaga ttgaaacaga
     2101 tacacaaaaa cccaagtata ttgagactgt atggggggcc ggatacagat ttaatttaca
     2161 ataaaaatat tgtttggatt ctgtttttat gttacaatta attttgaata cataaaggga
     2221 gtctctcaat gtacaaatat tcagtgaaat tggagaaaat aattgaagaa tttcaactta
     2281 aggttctttg ctgtgaggag caggtcaaga ctattgaaat tgaaacaaaa gatataaaca
     2341 ggcctggtct tgaactagca ggttatacga accacttttc caatgacaga atccagctta
     2401 tgggaatggt tgagtactca tatttaaaag aactttccct gcagacaaga aagaaaaaaa
     2461 tcgacaaact cttttctgaa ggctttccat gccttattgt ggcaaggggc cttgaagttt
     2521 ttccagaact gattcaaatt gcaaacaaat acaacatacc tgtcatgagt caccatgaag
     2581 ccacaacgga actgttcagc tcattgaaca gatacctttc cgtacagctg gctccaagac
     2641 aaactctgca tgcaggcctt gtagaggttt atggtgaagg tgttctcatt atgggtgaaa
     2701 gcggagttgg aaaaagtgaa accgctcttg aactcgtaaa gagaggtcac cgcctggtgg
     2761 ctgatgacgt agtggaggtt aggagagtat ccaacaaaac cctggtaggt tcagcgcctg
     2821 agaccataag gcatttgata gagatacgtg gccttggttt catcgatatt agaagactgt
     2881 atggcatagg ttctgttaag ataacggaga acatcaagct ggttgtaaag ctggaaaact
     2941 ggcagaaggg caaggaatat gacagggtag gaataacgga tcaatatacg aatatacttg
     3001 gaatagaagt gccttcactg actatccctg taaggcctgg aagaaatctt gctgtcatag
     3061 ttgaagtagc tgccatgaac aacaggcaaa aggactttgg ct
//
LOCUS       DAONVY010000272         3090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21689_length_3090_cov_4.033608, whole genome shotgun sequence.
ACCESSION   DAONVY010000272 DAONVY010000000
VERSION     DAONVY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21689_length_3090_cov_4.033608"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1111)
                     /locus_tag="PLI11_07520"
     CDS             complement(125..1111)
                     /locus_tag="PLI11_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPZ52752.1"
                     /translation="MDKYNEKYYTKTERRSDFVRLRDLRKSVEQKKDYQENVVRIPLD
                     RLRCSPFQPRKIFQTLSLNELSESIREFGVLQPILVRDMGDGYYEIISGERRYRASLI
                     AGKTDIPAIVLSINDNEAAIHAIVENIQREDLDYMELAEAYNRLIVEHGMTQEEVARR
                     VGKSQSAIANKIRLLRLSPNVVKVLSEHGLTERHARALLRLPNEQLQLKVLKIVTDRA
                     FSVAETELLISKTLEAAKGDKKSDSYFSQGKGASKVQEKHPGVKDALNEIDSFISGIK
                     KSVAVFKQLGINAKTAQFDRDTYFEIVVRLPKELNGYKYYGMASENEVKSAE"
     gene            complement(1190..1912)
                     /gene="rsmG"
                     /locus_tag="PLI11_07525"
     CDS             complement(1190..1912)
                     /gene="rsmG"
                     /locus_tag="PLI11_07525"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPZ52753.1"
                     /translation="MNKKAVEMLYEGAKRFGIQLSDEQVTMFRSYSSMLKERNKVVNL
                     TAITEDEDIAVKHFLDSLTLYPYIQELKGKSLVDIGTGAGFPGIPLKIVCPDLKITLV
                     DSLIKRVAFLNDVVDELGLKDINAIHARAEEIGKNKEHRERYDFATARAVAQMPVLLE
                     YCIPLVKVNGYFLAMKGAKEEDNYEKALEVLGGIFYKKEFLELEKDDEVIQRIIYIFK
                     KDKKTSTNYPRKPGVPTKKPLI"
     gene            complement(1912..>3090)
                     /gene="mnmG"
                     /locus_tag="PLI11_07530"
     CDS             complement(1912..>3090)
                     /gene="mnmG"
                     /locus_tag="PLI11_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPZ52754.1"
                     /translation="WLTHTNIKTKEIILENIHRSPMYNGSIEGIGPRYCPSLEDKMVR
                     FSDKDRHQVFVEPMGADTEEMYLQGMSSSLPEDVQIEFMRSVEGLENVSVMRPAYAIE
                     YDAIDARVLKLTLESRFVDGLYFAGQINGSSGYEEAAAQGLVAGINCALKLQGKDPLF
                     IDRSEAYIGVLIDDLVTRGTREPYRMMTSASEYRLLLRQDNADERLTEKGYKVGLISE
                     ERYANFKEKYRLVKVELDRLSKTHVSPTPSVIEYMEKIGSTPLKSGASLEDLLKRPEV
                     DYEDVLAIEELNLKDKGLDYERKYLPKSVIEQVCINVKYSGYIKLQLQQVEQFKKLEK
                     KLLPEDIDYDEISNLSMEAREKLSKVRPASIGQASRILGVSPADITALYIYLTRRKEK
                     "
BASE COUNT          823 a          745 c          479 g         1043 t
ORIGIN      
        1 atggtatgtt tgataatatg tgctttcttt tcttctcaat ttatacctcc agaaaaatat
       61 ttagtattaa aaaaggaact gctcgttcaa acagttccta gaatgaaaat aagaccatca
      121 gttactactc cgcactttta acttcgtttt cgcttgccat tccataatac ttgtacccgt
      181 taagctcttt aggtagtctt acaactattt caaaatatgt atccctgtca aattgtgctg
      241 tttttgcatt aatgccaagc tgtttgaaaa ctgcaacact tttctttatg cccgagatga
      301 aggaatcaat ctcgtttaac gcatccttga cgccaggatg tttttcctgc accttgctgg
      361 ctccttttcc ctgggaaaag taactatcgc tcttcttatc acccttcgca gcttccagtg
      421 tcttggaaat taacaactcc gtctcggcta ccgaaaacgc cctgtctgta actatcttca
      481 atactttcag ctgaagctgt tcattcggca gtctgagaag agcccttgca tgtctctcag
      541 taaggccgtg ttcgcttaaa accttaacca cattcggaga aagcctcaaa agccttatct
      601 tattggctat tgcagactga ctttttccaa ctcttcttgc cacttcttcc tgtgtcattc
      661 catgctcaac tattaatcta ttgtatgctt cagctagttc catgtaatca agatcttctc
      721 gttgaatgtt ttccacaatt gcgtgtattg ctgcctcgtt gtcattaata cttaatacaa
      781 tcgcaggaat atctgttttt cctgctatta gagatgccct gtaccttctt tcgccagata
      841 taatctcata ataaccatcg cccatatccc tgacaagaat aggctgaaga actccaaact
      901 ccctaattga ctcagacaat tcattcaagg acaaagtttg aaaaatcttt cgcggttgaa
      961 aaggactaca tctgagacga tcaagaggta ttcgaactac attttcctgg taatccttct
     1021 tttgttcaac gctctttctc aagtctctca gtctcacaaa atcacttctt ctttcagttt
     1081 ttgtatagta tttttcatta tacttgtcca catatatttt tcaacactac acaaaagtga
     1141 gaaacttgat tttttcgaca ttttcgacat gcttcgacaa aaatacctat caaataagag
     1201 gcttttttgt cggtacacca ggttttcttg gataatttgt cgaagtcttt ttatcctttt
     1261 tgaaaatata tattattctt tgtataactt catcgtcttt ttccagctca agaaattcct
     1321 ttttataaaa tattccaccc agtacttcca acgctttttc gtagttatcc tcttcttttg
     1381 cccccttcat ggccagaaaa taaccgttaa cctttacaag tggaatacag tactcaagca
     1441 gtacaggcat ttgtgcaaca gccctggctg tagcaaaatc atacctttcc ctatgttctt
     1501 tattctttcc tatctcttca gctctggcat gaatggcatt aatatccttg aggccaagct
     1561 cgtccaccac atcatttaaa aaagccaccc tttttatcaa ggagtccaca agggtaattt
     1621 tcaaatccgg gcaaactatt ttcagaggta tgccgggaaa acctgcaccc gtacctatgt
     1681 caacaaggct tttaccctta agctcctgta tatatggata caaggtaaga gaatcaagaa
     1741 agtgcttcac tgcaatatcc tcatcttcag taatagcagt aagatttaca accttgtttc
     1801 tttccttaag catcgaagaa taggagcgaa acatcgtcac ctgttcatcc gacagttgta
     1861 taccaaacct ttttgcacct tcgtaaagca tctcaacagc ctttttattc attatttttc
     1921 cttccttctt gtcagataga tatacaaagc ggtaatatcc gcaggagata ctcccagtat
     1981 cctgcttgcc tgtccgatgg aggcaggcct tactttactt agcttctctc ttgcctccat
     2041 gcttagattg ctgatttcat cgtaatcaat gtcttcaggt agaagtttct tctccagctt
     2101 tttgaactgc tccacctgct gaagctgaag ctttatatat cctgagtatt tgacgttgat
     2161 acacacctgt tctattacgc ttttaggtag atactttctt tcgtaatcaa gacccttgtc
     2221 ttttaagttg agttcctcaa ttgccaacac atcttcatag tcaacttcag gtcttttcaa
     2281 aagatcttcc aacgaagcac cgcttttcaa aggagttgac cctattttct ccatatactc
     2341 tattacactt ggggtaggag atacatgtgt ctttgacaac ctgtcaagtt ctacttttac
     2401 aagcctgtat ttttccttaa agttagcata tctttcttca gatatcagtc cgactttgta
     2461 tcctttctca gtaagtcttt catccgcatt atcctgcctt aaaagaagcc tgtattcact
     2521 tgctgatgtc atcattctgt aaggctccct tgtgcctctt gttaccaggt catctatgag
     2581 tacacctata tatgcttcgg atctgtcaat aaaaagaggg tcctttccct gaagcttcaa
     2641 ggcacagttt atgcccgcaa caagaccttg ggcggcagct tcctcataac ctgatgaacc
     2701 gtttatctgt ccggcaaaat acagtccatc cacgaacctg ctctcaagag tcagtttcaa
     2761 aacccttgca tctattgcat catattctat tgcatatgca ggcctcataa cggaaacatt
     2821 ctcaagacct tcaacacttc tcataaactc aatctgtaca tcctcaggaa ggcttgaaga
     2881 catgccctga agatacatct cttccgtatc ggcacccatt ggctcaacaa aaacctgatg
     2941 cctgtccttg tctgaaaacc taaccatctt atcttccaat gaaggacaat acctaggacc
     3001 aattccttca atagatccgt tgtacattgg agaccggtgt atattttcaa gtattatttc
     3061 ctttgtcttt atattagtat gggtaagcca
//
LOCUS       DAONVY010000273         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21728_length_3086_cov_2.649621, whole genome shotgun sequence.
ACCESSION   DAONVY010000273 DAONVY010000000
VERSION     DAONVY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21728_length_3086_cov_2.649621"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLI11_07535"
     CDS             <1..1164
                     /locus_tag="PLI11_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52755.1"
                     /translation="GSEQTVYDFCVSDTSVDGSKSFVYNVEFEKESGYGAGILLGFKD
                     SSSRKGILDSFIYFIADPDNVYYSVFENGNMVAGPVGRPLYEEEKSSDMVLTCQYIKQ
                     TETCVFYINGNYVGMYANKDKVAGKMGLVAHDASVYFTKAKLMVLDDSKEETRFETNM
                     TGWKGVESSWKETDVGYIDGNEQNAYDFAMSDIYVDGTKSFVYKVEMARMGGYGFGIV
                     FGASSSDERNTILNNSTLFLVDTRSVLYNRNQQENVAKPLGEELKDPLLKEFKEDSKV
                     YKLRLEYHAEDEMAILYLDDLDVFVIDDPEFVVGYLGVFAHEGNARVLSAKFEEMAET
                     DETEKPVKTQSPEPSEKVEEKDEKGSMLYIWIGLGVLLIVLAFIFVIVKRRKG"
     gene            1168..2358
                     /locus_tag="PLI11_07540"
     CDS             1168..2358
                     /locus_tag="PLI11_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52756.1"
                     /translation="MSKTLLLLIKFLVLLAFVTSCDKVDKTPKRESKKEEIPEITFTE
                     DGPRFRNRKLTEHFTVNDSPILYGFYNLYNFEVVKVDDKEYPYRMFFFGESHKESKYI
                     GYDSIYLARGKSLVSWEVYCGNDTWDDTMDVPKWEPVMTRDDKPYDSIHNGDPSVVYK
                     DGTYYMAFSSVGFDSRDGITYIVNCVMGAVSKDGINWTKTEGPILIWDKEYEEGWDAR
                     EPHPPSMGGYHRPSLVWDEEEDKWKIWFDYYLPGTFLSMGYAVNNGEFMDPTHWQVLH
                     ADENPQLRDWPNPEVVKINGRYYSFSDCTGFGSSLGPQNDRQIVMAYSNNGYEWKVIG
                     RILPEDKIYGTHLPQTFVEQVDGEKILYIFYSMVVEEDLPRYSKANFMYIKVEDLENL
                     VDSY"
     gene            complement(2680..2856)
                     /locus_tag="PLI11_07545"
     CDS             complement(2680..2856)
                     /locus_tag="PLI11_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52757.1"
                     /translation="MLINILFWANIIWVAPLVYFMLANETKFKKNIAVGVTMPLEGRS
                     DSFYLSPYPSQVQF"
BASE COUNT          932 a          511 c          757 g          886 t
ORIGIN      
        1 ggaagtgagc agactgtata tgatttttgt gtcagtgaca cgtctgttga tggcagcaag
       61 agttttgtct acaatgtgga gtttgagaag gaaagcggct acggagctgg aatactttta
      121 ggtttcaaag attcttccag tagaaaagga atccttgact cctttatcta tttcattgcc
      181 gatccagaca atgtatacta cagtgtgttt gaaaatggaa atatggttgc aggacctgtg
      241 ggaaggcctt tgtatgagga ggaaaagagt tctgacatgg ttctaacctg tcagtacata
      301 aagcaaacag agacttgtgt attctatatt aacggaaact atgtgggaat gtatgcaaac
      361 aaggataagg tggcagggaa aatgggtctt gtagcccatg atgcttccgt atactttaca
      421 aaggctaaac ttatggtgct tgatgacagc aaggaagaga caaggtttga aacaaacatg
      481 acaggatgga aaggtgtgga gtcttcctgg aaggaaactg atgtgggata tattgatgga
      541 aacgagcaaa acgcttatga ctttgccatg tcggatattt atgtggatgg tacgaaatct
      601 tttgtttaca aagttgagat ggcaagaatg ggcggctatg ggtttggcat cgtgtttgga
      661 gcgtcaagca gtgacgaaag aaacacaata ctgaataaca gcaccctttt tcttgtggat
      721 acaagaagtg tactgtataa cagaaaccag caggagaatg tggcaaaacc tttgggtgag
      781 gaactaaagg atcctctcct gaaagaattc aaggaagact ccaaagtgta caaactaaga
      841 ctggagtacc atgcggagga tgaaatggca attttgtatc ttgatgatct tgatgtgttt
      901 gttatagatg atccggagtt tgtcgttgga tatttgggtg tgtttgcaca tgagggaaat
      961 gcaagggtat tgagtgccaa gtttgaggaa atggcagaaa ctgatgaaac tgaaaaacca
     1021 gtaaaaaccc aaagtcctga accgagtgag aaagtggagg aaaaagatga aaagggcagc
     1081 atgctctaca tatggattgg cttaggtgtg ttgttgattg ttttggcttt tatttttgta
     1141 attgtaaaga gaagaaaggg gtaaatcatg agcaagacat tattgcttct aatcaagttt
     1201 ttagtacttt tggcgtttgt aacgagctgt gacaaggttg acaagacgcc aaaaagagag
     1261 agtaaaaaag aggagattcc tgaaattact tttactgaag atggtccaag gttcagaaac
     1321 agaaagctga ctgagcattt tactgtcaat gacagtccta tcctctacgg tttttacaac
     1381 ctctacaact ttgaggttgt aaaggtggat gacaaggagt acccatacag aatgttcttc
     1441 tttggagaga gccacaagga aagcaaatac attggatatg actccatcta ccttgcccgt
     1501 ggaaagagcc ttgtttcatg ggaagtgtat tgtggaaatg atacctggga cgatactatg
     1561 gacgttccaa agtgggaacc tgttatgaca agggacgata aaccttacga ttcaatacac
     1621 aacggggacc cttccgttgt atacaaggat ggcacttact acatggcttt ttcttccgta
     1681 ggatttgata gcagggatgg cattacgtat attgtaaatt gtgtgatggg agctgtatct
     1741 aaagacggaa tcaactggac aaaaacagaa ggcccaatac tcatatggga caaggagtat
     1801 gaagaaggat gggatgcaag agagcctcat ccgcccagca tgggaggata ccacagaccg
     1861 tctcttgtat gggatgagga agaggacaag tggaagatct ggttcgacta ctacctgcca
     1921 ggcacattcc tttccatggg ctatgcggtc aataatggag agttcatgga tccgacccac
     1981 tggcaggtgt tgcatgcgga tgagaatcca cagctcagag actggcccaa tcctgaagtt
     2041 gtaaagatta atggaaggta ctactctttt tcagactgca ccggatttgg ttcttctttg
     2101 ggacctcaaa atgacaggca gattgtcatg gcgtattcca acaacggtta cgagtggaag
     2161 gttattggaa ggatacttcc tgaagacaag atatatggaa ctcatcttcc acaaaccttt
     2221 gtggaacaag tggatggaga gaagattttg tacatcttct actccatggt ggtggaagaa
     2281 gatcttccaa gatactcaaa agccaatttc atgtatatca aggttgaaga tttggagaat
     2341 cttgtagatt cttactaata tcttcataaa tggttcttgt gtgttcttca tctcttgacc
     2401 caaacagata ccttttatta tcctttgttg taacaaggat gaaaggtgtg tgtcttgggt
     2461 caagaatcaa ttgaaccttt ccaactcctt ttaaaatgta gttgcctttt gaaagattct
     2521 ccatggcagt tccgttaact cttgtaatgt cgggtttttc ttcaagtagt tcaacctttt
     2581 ctatatcatc aaagctgaca gaaaattttg tttggccatg ttttgccgta atgtggttgt
     2641 cctttacata cagttcaata ggtccttttc ctatctggct tagaattgga cctgtgaagg
     2701 gtatggcgat aagtaaaagg aatcacttct tccttccaaa ggcatggtga ctccaacggc
     2761 gatattcttt ttgaatttgg tttcgtttgc cagcatgaag tatacaaggg gtgcgaccca
     2821 tattatgttt gcccagaaaa gtatgttgat aagcatgcag tgtccctcca atattgatat
     2881 attactattt tatcacagaa caagctgttt tgcagattga aaactgcttg tttaaggaag
     2941 ttggagtggc gtgtcttgga taagagaccc taattcacaa gatatgagat atgttagaga
     3001 gttaatgtat tcctgaaatc aagcggaagc gccacttcca acttccttaa tcgcaaggag
     3061 agagtaaaaa caaggattta tatttt
//
LOCUS       DAONVY010000274         3082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21756_length_3082_cov_3.453254, whole genome shotgun sequence.
ACCESSION   DAONVY010000274 DAONVY010000000
VERSION     DAONVY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21756_length_3082_cov_3.453254"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PLI11_07550"
     CDS             <1..1419
                     /locus_tag="PLI11_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPZ52758.1"
                     /translation="INPESITDYEEKGGYEALRKVLTEMTPEEVIEEIKKSGLGGRGG
                     AGFPTWFKWNAARSSMEEPKYLICNADEGDPGAFMDRAIIEGDPHTLIEGMLIGAYAI
                     GAAEAVVYVRAEYPLAIIRLNKAISQAVEKGYLGRNIFGTSFSCHIRIKAGAGAFVCG
                     EETALIESLEGQRRMPRVKPPFPAAKGYWQKPSNINNVETFANVAWIILNGGDAFSAM
                     GTQDSKGTKVFALTGKINKGGLVEIPMGKTLRDVIYDIGGGIKENKRFKAVQMGGPSG
                     GCIPEDYLDTVIDYKKLSQTGAIMGSGGMVVMDETNCMVDMARFFLDFTAKESCGKCI
                     HCRIGTKRMLEILNRIVVGEGKDGDIELLEEMCYSIKDGSLCGLGQTAPNPVLTTIKY
                     FRNEYEAHIYHKKCPAKQCKSLITYRIDEDRCIGCRVCARNCPVNAISGNLKEVHVID
                     SETCTKCGKCMEVCRFKAVVCE"
     gene            1439..>3082
                     /locus_tag="PLI11_07555"
     CDS             1439..>3082
                     /locus_tag="PLI11_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ52759.1"
                     /translation="MLKVKINGNEYIGEKGQTILDIAKSNGVEIPTLCHHEKVKPYGG
                     CGLCVVEIKGFGKLARACATEAADGMDINTVSDRVVQARKIALEFLLSDHVGDCRPPC
                     MLACPANTDCQGYVGLIANGMYKESGDLINERLPMPASIGRVCPHPCETACRRGALDE
                     PVAIAWLKRFVGDVNLANKQVDFKSKATIGTGKRVAVIGGGPAGLSCAYFLRMNGYAV
                     TIYEAMPELGGMLMYGIPEYRLPKEVLKKEIDMILSMGVQVKTNTKIGCDISLDDIKK
                     EYDAVIVAIGAWKSSRMNIPGEDLHGVYGGIDFLRNVAMSDVQHESRDKIVSSLSIGK
                     RVAVVGGGNTAMDACRTAVRVGASEVYTLYRRTREEMPADPVEVEEAMEEGVIFKFLS
                     NPVEIIGEDNKVKRIKVQKMKLGEPDASGRRSPVPIEGAFEELEVDAVIIAIGQSIDI
                     EGFESLQTTRKRTIIADENTFMTSMEGVFACGDVTNAGPDIAIAAIGEAQKAAYSVHK
                     YLGGEIDHIFGNIKEYDFYSKREVTPEDIREIFKDREIEY"
BASE COUNT          966 a          539 c          846 g          731 t
ORIGIN      
        1 attaatcctg aaagcataac tgattatgag gaaaaaggcg gatatgaagc tcttaggaaa
       61 gttcttactg aaatgactcc cgaggaagtt attgaagaaa taaagaagtc gggtctcgga
      121 gggagaggag gagccggatt tccaacttgg ttcaaatgga atgctgcaag aagttccatg
      181 gaagagccca aataccttat ctgcaatgcg gatgagggcg atccgggagc gttcatggac
      241 cgagccatta ttgaaggtga cccacataca ttgattgaag gcatgctcat tggtgcgtat
      301 gcgataggag cagcagaagc ggttgtgtat gtaagggctg agtatccttt ggcaataatc
      361 agactcaata aagcaataag ccaggctgtg gagaaaggct atcttggaag gaatatcttt
      421 ggaacaagct tcagctgtca cataaggatt aaagcaggcg caggtgcgtt tgtctgcggt
      481 gaggaaactg ctctgattga atccctggaa gggcagaggc ggatgccaag agtgaaacct
      541 cccttccccg ctgcaaaagg ttactggcag aaaccaagca acataaacaa tgtagaaaca
      601 tttgcaaatg tcgcttggat aatcctcaat ggtggggatg ccttttcagc catgggaacg
      661 caggacagta aaggcacaaa agtatttgct ttgactggaa agataaacaa aggtggtctt
      721 gttgaaattc ccatgggcaa aaccctgagg gatgtaatat atgatattgg tggaggaatc
      781 aaagagaata agagattcaa ggcagtgcag atgggcggac cttcaggagg atgcatccct
      841 gaagactatt tggatactgt aatagattac aagaagcttt ctcaaaccgg agccatcatg
      901 ggatccggcg gcatggttgt aatggatgaa accaactgca tggtagatat ggccaggttc
      961 ttcctggact ttactgcaaa agagtcctgt ggtaaatgca tccactgcag aattggaaca
     1021 aagagaatgc ttgagatttt gaaccgcatt gtagtcggag aaggaaaaga tggagatata
     1081 gagctcctcg aggaaatgtg ctactcgata aaggatggct ctctttgtgg tcttggccag
     1141 actgcgccaa accccgtact tacgaccatt aagtatttta gaaatgagta tgaagcacat
     1201 atataccata aaaagtgccc ggccaaacag tgcaaatcac taattacata cagaatcgac
     1261 gaagacagat gtattggctg cagggtctgt gcgaggaact gtccagtcaa tgctatttca
     1321 ggcaacctta aagaagtgca tgtaattgac agcgaaacat gcacaaaatg cgggaaatgc
     1381 atggaagttt gccgtttcaa ggcagttgta tgcgagtagt tgaaggatga gggaaaacat
     1441 gttgaaagta aagataaatg gcaatgaata tattggagaa aaaggccaga ccattctgga
     1501 tatagcgaaa tcaaatggtg tggagattcc gacactatgc caccatgaga aagtaaaacc
     1561 atatggtggc tgcggacttt gtgtagtaga aatcaaggga tttggtaagc ttgcgagagc
     1621 ttgtgcgaca gaagcggcag atggcatgga cattaatacc gtgtctgaca gggtggtgca
     1681 ggcgaggaaa atagctctgg agttcttact gtcagaccat gttggggact gcagacctcc
     1741 ctgcatgctt gcatgtcctg caaacacgga ctgtcaggga tatgtcggcc ttatagcaaa
     1801 cggtatgtac aaggagtccg gagacctaat caatgaaagg ctacccatgc cggcatccat
     1861 aggaagagta tgtccccatc cctgcgaaac tgcatgtaga aggggagcgc ttgatgagcc
     1921 tgttgccata gcatggctta agagatttgt aggtgatgtg aatcttgcca acaaacaagt
     1981 cgatttcaag tcaaaagcaa caatcggtac tggcaagagg gtggcagtta taggtggagg
     2041 ccctgctggc ttgtcgtgtg cgtattttct tcgcatgaat ggttatgcag tgacaatata
     2101 tgaagcgatg cctgaacttg gcggtatgct aatgtatgga attccagagt acagactgcc
     2161 caaggaagtt ctgaaaaaag aaattgacat gattctttcc atgggagtgc aagtaaaaac
     2221 gaacactaaa attggttgcg acatttcact tgatgatata aaaaaagaat atgatgcggt
     2281 gattgttgca attggagcct ggaaaagctc aagaatgaat attccgggtg aagatctgca
     2341 tggagtgtac ggtggtattg atttcttgcg taatgttgca atgtccgatg ttcagcatga
     2401 gagtagggat aagattgtat cttcattgag tattggcaag agagtggccg ttgtaggtgg
     2461 tggaaacaca gcaatggacg cctgcaggac agcagttaga gtaggagctt cagaggtcta
     2521 cactctatat agaagaacca gagaggaaat gccggctgac ccggttgagg ttgaggaagc
     2581 catggaagag ggcgtcattt ttaagtttct ttccaatcct gtggagatta ttggagaaga
     2641 taacaaagta aaaagaataa aggttcaaaa gatgaagctg ggcgagccgg atgcgtctgg
     2701 cagaagatca cccgtaccca tagaaggtgc atttgaggaa ctggaagtgg atgcggtaat
     2761 aattgcaata ggacagagca ttgatattga gggatttgaa tcattgcaga ccaccaggaa
     2821 gcgaacaatc atagctgatg aaaatacatt tatgacaagc atggagggtg tgtttgcatg
     2881 cggagatgtg acaaatgcag gacctgatat tgcaattgca gcaattggag aagcgcagaa
     2941 ggctgcctat tccgttcaca aataccttgg tggcgagata gaccacatct ttggcaatat
     3001 caaggaatat gacttttact ccaagaggga agttactccg gaagacataa gagagatatt
     3061 taaagacagg gaaatcgagt ac
//
LOCUS       DAONVY010000275         3080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21768_length_3080_cov_2.989091, whole genome shotgun sequence.
ACCESSION   DAONVY010000275 DAONVY010000000
VERSION     DAONVY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21768_length_3080_cov_2.989091"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="PLI11_07560"
     CDS             complement(<1..1780)
                     /locus_tag="PLI11_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52760.1"
                     /translation="MNKELLKEFKNPGVAYRGKPFWSWNGELEKDELVRQVKVMKEMG
                     LGGYFMHSRAGLITEYLGDEWFDLINAVADAGEEEGLESWLYDEDRWPSGSAGGKVTE
                     DPQYSMKSIVLKEFDVDKFSWDGDIVKVFAGKVDGFNLDEYFEISKDTDLSKLEGYKI
                     LKFEIIPQERNSVYNGNTYIDTMSLKATERFIELTHEEYKKRCEDRIGTTIKGIFTDE
                     PHRGHMLDDFKEVDGVRQCSCAYTDDIFEEFEKRYGYDAKAILPELYYIYKGRKVNKV
                     KKDYVDLANALFIERFAIPVNNWCIENNMELTGHYLHEDSLTNQTVPCGSIMRMYEYM
                     GYPGVDVLTEGNRCYWVVKQLSSVARQLDKKWLLSELYGCTGWQFDFKAHKAVGDWQA
                     LFGINLRCHHLSWYTMEGESKRDYPASILHQSAYYPYYDLVESYFARFGVVISSGKPE
                     CEVLVLNPIESVWAQIYMGWARWISSVDEDVRKLERHYEELFHMLCGNHIDFDYGEEE
                     MMSRHANVEDGVLRVGNAKYKVVVVSGMLTMRESTLKLLEDFVEQGGKVIFAGDLPKY
                     VDAVESEKVSELSSKATCVSFDGKVLA"
     gene            1885..2787
                     /gene="ftcD"
                     /locus_tag="PLI11_07565"
     CDS             1885..2787
                     /gene="ftcD"
                     /locus_tag="PLI11_07565"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPZ52761.1"
                     /translation="MNKIVECIPNFSEGRDSNIIKEIEEAIINTKNVILLEHSSDYDH
                     NRSVFTFMGSMEGVYDAMYKATSVAVNSIDLTKHEGKHPRIGAVDVIPLVPVQNVTME
                     ECVELSKTLAERIYNELGLPVYLYENSASKPHRTNLADIRRGEFEGLVEKMQSEDWKP
                     DYGTKPHPTAGAVVIGARKPLIAFNMQLNTANIEIATEIAKKIREKNGGLKCVKALGI
                     MLETKNTAQVSVNLTDYTVTSLFEVFDTVCNEASKMGYEVVSSELIGLSPARAFIDTA
                     ARYLKLENYSYKKIIESHLLNLEI"
     gene            2818..3039
                     /locus_tag="PLI11_07570"
     CDS             2818..3039
                     /locus_tag="PLI11_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HPZ52762.1"
                     /translation="MTVGGYVLKINLGDIIVFKKPHPCGGAEWEVLRTGADFRLKCVK
                     CNHQIMISRHKAAKRIMKIIPRDDQTKNE"
BASE COUNT          940 a          669 c          547 g          924 t
ORIGIN      
        1 ctgccagtac tttaccatca aatgaaacgc atgttgcctt tgatgaaagt tctgaaacct
       61 tttcagactc aactgcatcc acatacttgg gcaaatcacc tgcaaaaatt acttttccac
      121 cttgttctac gaaatcttcc agcaatttca acgtgctttc cctcatggta agcatgccgc
      181 ttactactac aaccttgtat tttgcattgc caactctaag cacaccatct tccacatttg
      241 catgccttga catcatctct tcctcaccat agtcaaaatc tatatggttg ccgcaaagca
      301 tatggaagag ttcttcgtag tgacgctcaa gcttcctaac atcttcatcc acactgctta
      361 tccatcttgc ccatcccatg tatatctgag cccatacgct ttctattgga tttagaacca
      421 gcacttcgca ctctggcttt cctgaagata ttacaacgcc aaatcttgcg aaatagcttt
      481 caacaaggtc ataatatgga tagtatgcgg actgatgcag aatgctggca ggatagtccc
      541 tctttgactc accctccatt gtataccagg acagatgatg gcatcttaag tttattccaa
      601 acagcgcctg ccagtcacca actgccttgt gggctttgaa atcaaactgc catccagtac
      661 atccgtacaa ctccgacaaa agccatttct tgtccagctg ccttgctact gaagaaagct
      721 gttttactac ccagtagcac ctattgcctt ccgtcagaac gtcaacaccg ggataaccca
      781 tatattcata cattctcata atggaaccac aaggaacagt ctggtttgtc aatgagtcct
      841 cgtgtagata gtggccggta agttccatgt tgttttcaat gcaccagttg tttacaggta
      901 ttgcaaatct ttcgataaat agagcatttg caagatccac ataatctttt tttaccttgt
      961 ttactttcct gcctttatag atgtaataaa gttcaggaag gattgctttt gcatcatagc
     1021 catatctttt ttcgaattct tcgaatatgt catcggtata agcgcaggaa cactgtctta
     1081 caccgtcaac ttccttaaag tcgtccagca tgtgtcctct gtgaggttcg tcagtgaata
     1141 tgccttttat tgttgtgcca attctgtcct cacaacgttt tttatactct tcatgtgtca
     1201 actcaatgaa tctttcagta gccttcaggc tcattgtatc aatatatgtg ttgccgttat
     1261 atacgctatt gcgttcctga ggaataattt caaatttcag aatcttatac ccttcaagct
     1321 tggacaaatc cgtatctttt gaaatttcaa aatattcatc cagattgaat ccgtcaactt
     1381 tacctgcaaa aacttttaca atatctccgt cccaggaaaa cttgtcaacg tcgaattcct
     1441 ttagaactat acttttcatg ctgtattgag gatcttctgt aacctttcca cctgcacttc
     1501 ctgaaggcca tctgtcctcg tcgtacagcc atgactcaag accttcttcc tcacctgcat
     1561 cagccacagc attaataagg tcgaaccatt catctcccaa atattcggtg atgagtcccg
     1621 ctcttgaatg catgaagtat ccgccaagtc ccatctcctt catgactttt acttgtctaa
     1681 caagttcatc tttttccagt tctccattcc aggaccaaaa aggtttgcct ctatacgcta
     1741 caccagggtt cttaaattct ttcaatagct ccttattcat cttctcatcc tttcatgttt
     1801 tgtgaacata ttatccatga attaaactaa aaaatcaagt acactgttat tgtggtataa
     1861 tacaataaaa aaggggtatt gaacttgaac aaaattgttg aatgcatacc aaacttcagc
     1921 gaaggaagag acagtaatat tatcaaggaa atagaagaag caataattaa tacaaagaac
     1981 gtcatacttt tagaacacag ctccgattac gatcacaata gaagcgtctt tacttttatg
     2041 ggctcaatgg aaggagttta tgatgctatg tataaagcca cttccgttgc agttaacagt
     2101 attgacctta caaagcatga aggaaaacac cctagaatcg gagcggtgga tgttattcca
     2161 ctcgttcctg tacagaatgt taccatggaa gaatgtgtgg agctttcaaa aactcttgca
     2221 gaaagaatat ataacgagct gggtttgcca gtttaccttt acgaaaactc tgcatcgaaa
     2281 cctcatcgta caaatcttgc agatattaga cgtggagaat ttgaaggctt ggttgaaaaa
     2341 atgcagtcag aagactggaa accagactat ggcacaaaac ctcatccaac agcaggcgcg
     2401 gtagttatcg gagctagaaa accacttata gcattcaata tgcagctgaa tacagcaaat
     2461 atcgaaattg ctacggagat tgcaaaaaaa ataagagaga agaatggcgg attaaaatgt
     2521 gtaaaagctc ttggcattat gctggaaact aaaaacacag cccaagtgtc cgtaaacctt
     2581 acagactata cggttacatc cctgtttgaa gtatttgata cagtttgcaa cgaagcatca
     2641 aaaatgggat atgaagttgt ttcatccgaa cttattggcc taagtcctgc tagagccttt
     2701 attgatactg cagcaagata tttaaagctt gaaaactatt cctacaaaaa aattatagaa
     2761 tcacatttat taaatcttga aatttaggac tgttaattgt acaattagga tgataatatg
     2821 actgttggag gttatgtgtt gaaaattaat ttgggtgata ttatcgtttt taagaaaccg
     2881 catccctgcg gtggggctga atgggaagta ttaaggactg gtgcggactt caggcttaaa
     2941 tgcgtgaaat gcaaccacca gattatgata agcagacaca aggctgcgaa aagaataatg
     3001 aagatcatcc ctcgtgatga ccagaccaag aatgaatgaa tggagaaaat ttatgatgag
     3061 taatgataaa gataatgata
//
LOCUS       DAONVY010000276         3077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21794_length_3077_cov_3.823627, whole genome shotgun sequence.
ACCESSION   DAONVY010000276 DAONVY010000000
VERSION     DAONVY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21794_length_3077_cov_3.823627"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLI11_07575"
     CDS             complement(<1..492)
                     /locus_tag="PLI11_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52763.1"
                     /translation="MINLYCPFKGSRARIYRELKKQLVCSHVIATQEYTYGKSDDIED
                     NILILAEFAESQELDTRILPGYLLNCDFFIPDAPDVLKKILDREYTLASSRYLLVQLH
                     KKPSYLQELISEMGRFGIVLVQLHNLKNDKCVKDNTPFGMLNMLNAEDITGRNGKKAK
                     KTAF"
     gene            complement(540..1352)
                     /locus_tag="PLI11_07580"
     CDS             complement(540..1352)
                     /locus_tag="PLI11_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPZ52764.1"
                     /translation="MHKIKNIKYFLLDMDGTIYLDTTLIDGTMDFLNELEKQNKRAIY
                     ITNNSSKAVAQYIEKLQSLNIETTEDDFCTSAIALVHNLKKEKEGAKIFLLGTPSLEN
                     YIVESGFELVKDYYTEEDKRPDYVVMGFDTTLTYEKLRIACDYINDGVAYVATHPDMV
                     CPVGVGRSIPDAGAFMELIAAATGRRPSMIAGKPNPLIIEMVMEKENCSKDEIAVIGD
                     RLNTDVLCAINAGVMSICVLTGETTKELLEASDVKPDYVFDSIKDVYEIIKE"
     gene            complement(1357..3021)
                     /locus_tag="PLI11_07585"
     CDS             complement(1357..3021)
                     /locus_tag="PLI11_07585"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPZ52765.1"
                     /translation="MAKYVIGIDFGTLSARALLVHAKTGEEIASCEYKYPHGVMDEKL
                     PNGKKLDIDWALQHPQDYLDALARVVPRVLKIGEVSKDDVIGIGIDFTACTILPVFKD
                     GTPLCFLAEFMSNEHSYVKLWKHHAAQDKANRINDLANERDENWLIRYGGKISSEWAL
                     PKLMEVMDEAPEIFEKMDFFIEAGDWLTWLMCEEAKVNACCAGYKGLWNKREGYPSKE
                     FLKQLDPRLENIYETKLGLPVYPSYTRAGTLTERGAKLTTLNVGTPVSMANIDGHACL
                     PAARITGPGKMLLILGTSYVHKVLHSDEMPIPGICGVVEDGILPGFYGYEAGQCGGGD
                     HFAWFIENNVPYKYYEEAKNHGMDIYAYMRQKASLLKAGESGLVALDWWNGNRSVLVD
                     ADLSGLVVGMTLRTKPEEVYRALIEATAFGTKTIIDNFKISGIPITEIVAAGGIAKKD
                     PLIMQILSDVTNMPISVVHTSYASALGSAIYASVAAGSELGGYDDVYEAIGNMGKVDD
                     FVYKPNPENAKTYTKLYEEYKLLHDYFGRGGNDVMKRLKNIAKEIN"
BASE COUNT          805 a          705 c          557 g         1010 t
ORIGIN      
        1 aaaggcggtt tttttagctt ttttgccatt tcttccggtg atgtcttccg cattgagcat
       61 attcagcata ccaaaaggag tattgtcttt aacacattta tcatttttca ggttgtgaag
      121 ttgcaccaga acaatgccaa acctgcccat ctcggaaatg agttcctgga gataggaagg
      181 ttttttgtgc agctgaacaa gcagataccg agaagacgca agggtatact ccctgtcaag
      241 aatcttcttt agtacatctg gtgcatccgg tatgaagaaa tcgcagttta aaagataccc
      301 tggaagaatt cttgtatcta gttcctgact ctctgcaaat tctgcaagga taagaatatt
      361 gtcttcaata tcgtcacttt tcccataagt atattcctgc gttgcaatta catgggagca
      421 cacaagctgc ttcttcaatt ccctataaat ccttgccctg cttcctttaa acgggcagta
      481 caggttaatc atatgcttca cctcttgaaa aaacagccca ctttacgtgg actgtacttc
      541 tactctttaa taatttcata aacatctttt atactgtcaa acacataatc cggctttaca
      601 tccgatgcct ccagcagttc ttttgtggtt tcaccggtaa gaacacaaat tgacattact
      661 cctgcgttta tggcgcacag aacatctgta ttcaaacggt cgccaataac tgcaatttca
      721 tccttgctgc agttttcctt ctccataacc atttcaataa tcaatggatt gggttttcca
      781 gctatcatgg aaggtcttct gcctgtggca gcagctatca attccatgaa agcacctgca
      841 tctggtattg agcgcccaac acctacaggg cataccatgt ccgggtgggt tgctacataa
      901 gccacaccat catttatata atcgcaggct attctaagtt tttcatatgt cagggtggtg
      961 tcaaatccca taaccacata atctggtctt ttgtcttctt cggtatagta atcctttaca
     1021 agttcaaaac cgctttccac aatgtagttt tcaagagaag gagtaccaag caaaaagatt
     1081 ttcgctccct ctttttcttt cttcaaattg tgcacaagtg caattgcaga tgtgcaaaag
     1141 tcgtcttcag tagtttcaat gttcaaagac tgaagttttt cgatgtactg tgctacagct
     1201 tttgatgagt tgttggttat ataaatagct cttttgtttt gtttttcaag ttcatttaaa
     1261 aaatccatag ttccgtcaat caatgttgta tcaagatata ttgtcccgtc catatcaagt
     1321 aaaaagtact ttatgttttt gatcttgtgc attttatcag tttatctcct ttgcaatatt
     1381 ttttagtctt ttcattacat cgttgccgcc tcttccaaag tagtcatgca gcaatttata
     1441 ctcttcatac agttttgtgt atgtttttgc gttttcagga tttggtttgt atacaaagtc
     1501 atcaacttta cccatattgc caattgcctc ataaacgtcg tcatatccac ccaactcgct
     1561 gcctgctgcc acagatgcat atatcgcaga acctaaagcc gaagcatagg atgtatgcac
     1621 aacggatatg ggcatgttcg tcacatccga caaaatctgc ataattaaag gatccttctt
     1681 ggcaattcct ccagcagcca caatttcagt tattggtatg ccgcttatct tgaagttgtc
     1741 aattattgtt tttgttccaa aggcggtggc ttctatcaaa gccctgtaaa cttcctctgg
     1801 ttttgttctc aatgtcattc caactacaag accggacaag tcagcatcta caagaacgct
     1861 tctgtttcca ttccaccagt caagggctac aagaccgctt tcccctgctt tcaatagaga
     1921 tgctttctgt ctcatgtatg cataaatgtc cattccatga tttttggctt cttcataata
     1981 tttatatggt acattgtttt ctatgaacca ggcgaagtgg tctccgccgc cgcattgacc
     2041 agcttcataa ccgtaaaagc ctggaagtat cccgtcctcc acaacaccac agatgcctgg
     2101 gatgggcatt tcatcagaat ggagcacttt atgtacatag gatgttccga gtatcagaag
     2161 cattttgccg ggacctgtga ttcttgccgc aggtagacac gcatgaccat caatgtttgc
     2221 catggacaca ggtgtaccaa catttaaggt tgtcaatttt gctcctcttt cagtcagggt
     2281 tcctgctctg gtatatgaag ggtatactgg aagaccaagc ttggtttcat agatattctc
     2341 aagtctaggg tcaagctgtt ttaaaaactc cttgcttgga tagccttctc ttttgttcca
     2401 cagacctttg taacctgcgc agcatgcatt gacttttgct tcttcgcaca taagccaggt
     2461 aagccagtct cctgcctcta taaaaaagtc cattttctca aaaatctccg gagcttcatc
     2521 cattacttcc ataagctttg gaagagccca ttcagacgag atttttccac cataacgaat
     2581 aagccaattt tcgtctctct cattggcaag atcgtttatc ctgttggctt tgtcctgggc
     2641 tgcatggtgt ttccacagct ttacatagct gtgttcgttg gacatgaatt ccgccagaaa
     2701 gcaaagtggc gtgccgtctt tgaaaacggg aagaatcgtg caggctgtaa agtcaatacc
     2761 tataccgatg acatcatctt ttgatacttc tccaattttt agaaccctgg gtacaactct
     2821 tgctaatgca tccagatagt cttggggatg ttgaagtgcc cagtctatgt ccagcttttt
     2881 tccgtttgga agcttttcat ccatgacgcc gtgtggatac ttgtactcac agctggctat
     2941 ttcttctcca gtctttgcat gtaccagcaa agctcttgca gacaatgtac cgaaatctat
     3001 accaataaca tactttgcca taatattatc ctttctttaa attgataata cattatcatt
     3061 gtacactaaa atacaat
//
LOCUS       DAONVY010000277         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21806_length_3076_cov_2.966567, whole genome shotgun sequence.
ACCESSION   DAONVY010000277 DAONVY010000000
VERSION     DAONVY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21806_length_3076_cov_2.966567"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1276)
                     /locus_tag="PLI11_07590"
     CDS             complement(188..1276)
                     /locus_tag="PLI11_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52766.1"
                     /translation="MKNASLDGIGMLNGGEYNKIELDGISKLKHPLIAKSVSIDGIFK
                     SKAKIQADFLSFDGISRVFRDIKAKKIDINGIVKIRRANLYADEIICTGILVCNREVN
                     ADYINIDGNCSANTMYGDTILINSNENNNQNARIPSKLGIFSKLYFGRQVKTSMSLVD
                     RIECTHFEGNNVYCKEVYANSVKLTGYSEIEVLHCDGDIDVANTCTIGKIVGKEYNIR
                     EMGEMVNTTIRKILDLYKDGKIDANEAETMLSSLGVENKTHDSSPSVPWEDDGKLRVV
                     AYIGRKLLKKGDPGQSKISVSIEGDALNVEAHGNVECTDIRGSVSAAGNVRCNDIRGN
                     VSCSGNVQCSQINGNVSSSGGIRIDTGR"
     gene            complement(1273..2121)
                     /locus_tag="PLI11_07595"
     CDS             complement(1273..2121)
                     /locus_tag="PLI11_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52767.1"
                     /translation="MRSSSIAFGDRHYEGNYNRLAIAGDAEITNSDVKKVYCAGDLSI
                     SKSTIGNLKFAGDLEADSVTIGTIKGAGDASFSGLCKVDNCVMVGDLECDMLECEILQ
                     NGPRSERVNTTSTPTWEGVFKGETFENFKELALEGEIEFNNIILSAPLSTKTEISCDN
                     FYLFSGLTFGSINAEHTFILASSNVHLDTVTGCTVEVKKDFKPGKEFKRIRKNGKYNN
                     LCSDNGIASVDNIEADNIYLENTKSKYICGINVVIGDLCIVDRVEYQDTIEISHKAIV
                     NEVVKT"
     gene            complement(2118..2804)
                     /locus_tag="PLI11_07600"
     CDS             complement(2118..2804)
                     /locus_tag="PLI11_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HPZ52768.1"
                     /translation="MLISKKELLQETNISYGQLYRWKREGLIPEEWFIKQPTFTGQET
                     FFPKKKILRRIKKIQELKNQYSLEELARILAPEVSERNFTEQDLRIIEEVDLALIPCF
                     FKSFGKNNFTYVELLVLIALSIFRKRHNLSIDDVEMLSKGIKDAINELKQVDYICLLL
                     KSNGEYFLMLYPENTGVMTDERLKKISEINLNEISSSIKLKYRKSFHLDIEEEENELL
                     FIEGGEEVLT"
BASE COUNT          758 a          671 c          559 g         1088 t
ORIGIN      
        1 ttattattgt ccattttgat ttttctattc ataaaaagag ggagggggct atgaagcccc
       61 ctttgtaatg atatgtgaaa attggactag tttcttttat tgtagtagtt ttttagctca
      121 aattcacaaa gctcgtatac aagtattctt tccttgtggt ttagttttct cttttttctc
      181 attcttatca cctgcctgtg tcaatacgta tacctccgct ggatgaaaca ttgccattaa
      241 tttggctgca ctgtacgttt ccactgcagc ttacatttcc tcgaatatca ttgcagcgta
      301 catttcctgc tgcacttacg cttcccctga tatctgtaca ttcaacattc ccgtgagctt
      361 caacgtttaa tgcgtcgcct tcaatggaaa cgctgatctt gctttggcct gggtctcctt
      421 tttttagaag tttccttcca atgtaggcaa caacacgcag tttcccgtca tcttcccatg
      481 gaacacttgg agagctgtcg tgggttttgt tttcaactcc caaagagctc agcatggtct
      541 ccgcctcatt ggcatctatt tttccatcct tgtataaatc aagtatcttc cttatggttg
      601 tatttaccat ctctcccatc tcccttatgt tgtattcctt tcccacaatc ttgcctattg
      661 tgcatgtgtt ggcaacatca atgtcgccat cgcagtgaag cacttctatt tccgaatatc
      721 ccgtcagctt tacactgttg gcatacactt ccttgcaata cacattattg ccttcaaaat
      781 gagtgcattc aatgcgatct acaagggaca tgctcgtctt tacctgtctt ccaaagtata
      841 gtttgctaaa aataccaagc ttgcttggaa tccttgcgtt ttggttgttg ttttcgtttg
      901 aattaatcag aatcgtatct ccatacatgg tgtttgcgga gcagttgcca tcaatattta
      961 tgtaatcggc atttacttcc ctgttgcata ccagtatgcc ggtgcatatg atttcgtcag
     1021 cataaaggtt tgctctcctg attttcacaa ttccattaat gtctatcttt ttagctttga
     1081 tgtcccggaa cactctggaa ataccgtcaa agcttaagaa gtccgcttgt atttttgcct
     1141 ttgacttaaa aataccgtca atactaacgc ttttagcaat gagagggtgt tttagttttg
     1201 atattccatc aagctcaatc ttgttgtact caccaccgtt tagcatgcct ataccatcca
     1261 atgatgcgtt cttcatgttt taaccacctc gttcactatg gccttatggg atatttctat
     1321 ggtgtcctgg tactcaactc tgtcgacaat gcacaaatct ccaataacca cattaattcc
     1381 gcaaatatat ttgcttttag tgttctctaa atatatattg tctgcttcaa tattatctac
     1441 ggatgcaatg ccgttgtctg aacagaggtt gttgtatttt ccgttcttcc ttattctttt
     1501 aaactctttg cctggtttga agtccttttt tacttcgaca gtacagcctg ttacagtgtc
     1561 cagatgtaca ttcgaagagg caaggatgaa tgtgtgttct gcgtttatgc tgccaaatgt
     1621 caaaccgctg aaaaggtaga agttgtcgca ggagatttct gtttttgttg acaggggagc
     1681 tgataaaatt atattgttaa actcaatctc tccctccaaa gcaagctcct taaaattctc
     1741 aaaagtttct cctttgaata caccttccca tgtgggtgta ctggttgtat taactctttc
     1801 gcttctgggg ccgttttgca gaatttcgca ctccagcatg tcgcactcca aatcacccac
     1861 catgacacag ttgtcaacct tgcacagtcc tgaaaaggat gcgtctccgg ctccttttat
     1921 ggtgccaatg gtgaccgaat ccgcctcaag gtcgcctgca aatttcaaat ttccgatggt
     1981 agactttgaa atcgaaaggt cgccggcgca atatactttt tttacatcgc tgtttgtaat
     2041 ctcagcatcg cctgcaattg caagccggtt gtaattgcct tcataatgtc tgtcgccaaa
     2101 tgcaattgaa ctggatctca tgtcaggacc tcctctccgc cttcaataaa caaaagttca
     2161 ttttcctctt cttcaatatc caaatgaaag ctctttctgt atttcaactt gatggaagag
     2221 gagatctcgt taagatttat ctcacttatc tttttgagtc tttcatcagt cattactcct
     2281 gtgttctcag gataaagcat gaggaaatac tctccattgg atttcagcaa aagacagata
     2341 tagtctactt gtttgagttc gtttattgcg tcttttatac cttttgacaa catttcaacg
     2401 tcatcaattg aaaggttgtg tcttttcctg aagatggaca gcgcaatcag taccaacaac
     2461 tccacatagg tgaagttgtt tttgccaaag cttttaaaga aacaggggat tagagccaaa
     2521 tcaacttctt ctatgattct aaggtcctgc tcggtaaagt ttctttcaga tacttcgggg
     2581 gctagtattc tagctagttc ttccagggag tactgattct tgagttcctg tatctttttg
     2641 attctcctca atattttctt ttttgggaag aatgtttcct gtcctgtgaa tgtaggttgt
     2701 tttatgaacc attcttcggg tataaggccc tctcttttcc atcgatacag ctgtccgtag
     2761 gatatgtttg tttcttgaag caattctttt ttggatatca gcatagtgtc accgcctaac
     2821 gtaacaatgt tttatttatc aatagtgtaa cgtaacaatg ttacactgtc aatggtttta
     2881 aatgtaaaca aattgttaat aataccaaag ctttcaattt gcataaagat gggttttgag
     2941 atataatagt gtggttcaaa aacctgatcg gagggtttgc atgactaacg tgtattttat
     3001 acgccatgcc cagtcaactg ccaatgtaaa tggctactgc tatggcatat ccgaatgcga
     3061 tgttacggaa tacggc
//
LOCUS       DAONVY010000278         3074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21817_length_3074_cov_3.263332, whole genome shotgun sequence.
ACCESSION   DAONVY010000278 DAONVY010000000
VERSION     DAONVY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21817_length_3074_cov_3.263332"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /gene="mutS"
                     /locus_tag="PLI11_07605"
     CDS             complement(<1..1559)
                     /gene="mutS"
                     /locus_tag="PLI11_07605"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPZ52769.1"
                     /translation="MTEQLTPMMKQYMTIKEQVKDCILFFRLGDFYEMFFDDAIEASK
                     VLELVLTKRQCGLSESAPMCGVPYHAALSYISKLVSNGYKVAICEQVEDPATAKGIVK
                     REIVKIYTPGTIVEEQMLDQKSNNYIMCIYCFRGMYGISSADVSTGEMYCTTLSIGAS
                     INRLADEVARYSPKEVIINEDAKDLDRIKKLISNLTGAYISTIPEEEFDYDKGRLLIN
                     EYIKDQKISEYDLSVNSAGALYSYIMNTQKNNNLDHLLTINFYKIDEYMSIDASSRRN
                     LEITETMRDKKKKGSLLWVLDKTITAMGGRELKKWLEKPLLDIAEINKRLDGVAELKE
                     LFMTRSEIREILNGVYDIERLTGKISLGNVNARDLISLKQSLGQLPYVKNTISTCQSE
                     ILKEIYENLDCLEDIYELIDKAIVEEPPISVTEGGLIKDGYNNQVDLYRVAARDGKKW
                     LEDYESGQKEKTGIKNLKIGYNRVFGYYLEVTKANFDLVPEHYIRKQTLANSERYTTE
                     ELAKIEEAILEA"
     gene            complement(1593..1970)
                     /locus_tag="PLI11_07610"
     CDS             complement(1593..1970)
                     /locus_tag="PLI11_07610"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HPZ52770.1"
                     /translation="MDYMEAAAQLGQAIANSSEMKAWKDAEAALMEDEKGNTLMEEYR
                     DAQMKMVRASREGLGQEEIEKCREMLLQKQRELNEYELTKNYFHAKDVFENMMRNIND
                     VVSFYVNGSGGGCSGSCSGCSGC"
     gene            complement(1994..>3074)
                     /gene="miaB"
                     /locus_tag="PLI11_07615"
     CDS             complement(1994..>3074)
                     /gene="miaB"
                     /locus_tag="PLI11_07615"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPZ52771.1"
                     /translation="HVVQEIRKKYENVDLVFGTHNLHRFPELLHKCIFTNTRVFEISN
                     TDGEVVEGVPVLRDSDFKAWVTIMYGCNNFCSYCIVPYVRGRERSRAVCDILAEVVEL
                     DKAGIKEITLLGQNVNSYGHDRRDGVDFATLLKLICDSTSIPRIRFMTSHPKDLSDEL
                     IEVMANNERICRHLHLPVQSGSTRILEKMNRKYTKEKYLELVKKVKERIPGIALSTDI
                     IVGFPGETEEDFNETLSVVEEVRFDSAFTFIYSKRTGTPAARFEDQIPEEVAKERFNR
                     LLELQNSISREINESYVGRRVEVLVEGLSRTNDKKYTGRTEGNKVVNFDRMDENIDLS
                     GRIVTVRIDSAQTWSLNGTLVNSKN"
BASE COUNT          710 a          767 c          537 g         1060 t
ORIGIN      
        1 gcctcaagga tggcttcctc aatcttggcc agttcttccg ttgtatatct ttcactgttt
       61 gcaagcgtct gctttctgat ataatgttct ggaaccaaat cgaaattggc ctttgtcact
      121 tccagatagt atccaaagac cctgttgtat ccaattttca gattcttgat gccggtcttt
      181 tccttctgcc cgctttcata atcttcaagc catttctttc catcccttgc tgccaccctg
      241 tacaagtcca cctggttgtt gtatccatcc ttgatcaacc cgccttctgt tacacttatc
      301 ggcggctctt ccactatggc cttgtctatg agttcatata tgtcttcaag gcaatccagg
      361 ttttcatata tctccttgag tatttcggac tggcatgtac ttattgtgtt tttgacataa
      421 ggcagctgac ccagagactg ttttagcgat atcaaatccc tggcatttac attgcccaat
      481 gatatttttc ctgtgaggcg ttctatgtcg tatactccgt tcaggatttc gcgtatttcg
      541 cttcttgtca tgaacagttc tttcagttct gcaacgccgt caagtctttt gtttatttca
      601 gctatatcaa gcaagggttt ttccagccac ttttttagtt cacggcctcc cattgcagtt
      661 atggtcttgt ccaaaaccca taaaagggaa ccttttttct tcttgtccct catggtttcc
      721 gttatctcaa gattccttct tgaagatgcg tctatggaca tgtattcatc tattttataa
      781 aaattaatag tgagcaggtg atccaaattg ttgttttttt gcgtattcat tatgtacgag
      841 tacaaggcac ccgcactgtt tacggacaaa tcatactcgc taatcttttg atcctttatg
      901 tactcgttga taagtagtct gcccttgtcg tagtcaaatt cttcctcagg tattgtactt
      961 atgtacgctc ctgtcaggtt tgatatgagt ttttttattc tatccagatc ttttgcatct
     1021 tcattgatta tgacttcctt gggggagtat cttgccacct cgtccgcaag acggtttatg
     1081 ctggcgccta tggatagcgt cgtacagtac atctcgcccg tggatacgtc tgcggaactg
     1141 atgccataca tgcccctgaa gcaataaatg cacataatat agttgttgga tttctgatca
     1201 agcatctgct cctcgactat tgtgccggga gtatatattt ttacaatctc tctttttact
     1261 atgcctttag cagtggcagg atcctctacc tgctcacaga tggccacctt gtatccattt
     1321 gatacaagct tgcttatata cgacagagca gcatgataag gcacgccgca cataggggca
     1381 ctttcggaaa gtccgcactg acgttttgtc aatacgagtt ccagaacttt tgatgcttca
     1441 attgcatcgt caaagaacat ttcataaaag tcccctaacc tgaaaaacag tatacagtct
     1501 tttacctgct cctttatggt catatattgc ttcatcatag gagtcagctg ctctgtcatt
     1561 atatatttcc tccaaatttc agtattcagc aattagcaac cggaacaacc gctgcagctg
     1621 ccgctgcatc cgcctccgct gccgtttaca tagaatgata ctacgtcgtt gatgtttcgc
     1681 atcatgtttt caaatacatc cttggcatgg aaatagtttt ttgtcaactc gtactcattg
     1741 agttcccttt gcttctgaag aagcatctct ctgcatttct caatttcttc ctggccaagt
     1801 ccttctcttg atgctctaac catcttcatt tgagcatccc tgtattcctc cataagagta
     1861 tttccttttt cgtcctccat gagtgcagct tcagcatcct tccatgcctt catttcacta
     1921 ctgtttgcta ttgcctgccc cagctgggca gcagcttcca tgtaatccat aatttctatc
     1981 tccttttcat attttaattt ttagaattaa ccaaagttcc atttagcgac caggtctggg
     2041 cagaatctat ccttactgtt actatcctgc cagacaggtc gatattttca tccatgcggt
     2101 caaaatttac aaccttgttg ccttccgtgc gtccggtata cttcttgtca ttggtgcggc
     2161 ttaaaccttc taccaaaact tccactctcc tgccgacata tgattcattt atttccctac
     2221 ttatactatt ctgcagctcc agcagtctgt taaacctttc ttttgccact tcttcaggaa
     2281 tttgatcttc gaatcttgca gcaggagttc ctgtccgttt ggagtagatg aaggtaaaag
     2341 cactgtcaaa cctgacttcc tccaccacac tcagcgtctc attgaagtct tcttccgttt
     2401 cgccgggaaa ccctacaatg atgtctgttg acaaggcaat acctggaatc ctttctttta
     2461 cctttttaac caactcaagg tacttctcct tagtgtactt cctgttcatc ttttcaagta
     2521 ttcttgtact tccggactga acaggcagat gaaggtgcct gcatattctc tcgttgtttg
     2581 ccattacctc aatgagttca tccgataaat cctttggatg ggaagtcatg aaccttatcc
     2641 ttggaatact cgtactgtca catatgagtt tcagtaaagt tgcaaagtca acgccatccc
     2701 ttctgtcatg accgtaagag tttacattct gtccaaggag tgttatttcc tttatgcctg
     2761 ccttgtccag ttctaccact tccgcaagaa tgtcgcatac tgccctgctt ctttccctgc
     2821 ctcttacata gggaactatg cagtaagagc agaagttgtt gcatccatac attattgtta
     2881 cccatgcctt gaaatcacta tcccttaaaa ccggaactcc ttcgacaacc tcgccgtccg
     2941 tatttgagat ctcaaaaaca cgggtattgg taaaaatgca tttgtgtaaa agctcaggaa
     3001 acctgtgcaa attatgcgtt ccaaaaacaa ggtccacatt ttcgtacttt ttcctgattt
     3061 cctgaacaac atgt
//
LOCUS       DAONVY010000279         3061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21940_length_3061_cov_3.574850, whole genome shotgun sequence.
ACCESSION   DAONVY010000279 DAONVY010000000
VERSION     DAONVY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21940_length_3061_cov_3.574850"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1783
                     /gene="gpmI"
                     /locus_tag="PLI11_07620"
     CDS             152..1783
                     /gene="gpmI"
                     /locus_tag="PLI11_07620"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPZ52772.1"
                     /translation="MIKYKGPVVTIIMDGIGLNNTVEGNAVKNAYTPTLDRLMQNYPM
                     VQLRAHGTAVGLPSDEDMGNSEVGHNAIGAGSVYSQGAKLVNESIESGKMFKGKGYIE
                     ILENVLKHNSTLHFIGLFSDGNVHSHIDHLKAMIKRAKQDGVKRVRVHILLDGRDVGE
                     TSALEYVIPFEEFLSGINGPDFDARIASGGGRMNITMDRYEADWAMVERGWKIHVLGE
                     GRMFSSSEEAIKTYREELHVIDQDLPGFVIAENGEPVGRIVDNDSVVFFNFRGDRAIE
                     ISRAFEEPDFDKFDRVYFPKVCYAGMLEYDGDLHIPSRFLVEPPHIQNTMGELFAKKG
                     ITQLAISETQKFGHVTYFWNGNRSGKFDDELETYVEIPSDIVPFEQRPWMKASEITDR
                     LIKELRTNKYKYARVNFANGDMVGHTGNFLAARIAVETVDLCLARILPVIDELGGMAI
                     ITADHGNADEMYEVKKGKLVTDENGKPKAKTAHTLNPVPCIFYDNTENRNKYKVKEGF
                     DKDGRIRFGLSNIAATCAFLLGVEKHESWDESIIE"
     gene            1864..2313
                     /locus_tag="PLI11_07625"
     CDS             1864..2313
                     /locus_tag="PLI11_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPZ52773.1"
                     /translation="MKNNTLLLSTILVVGIAVTTVVIWYILRSGSCESAGTVQVREGK
                     YMKLAPSVAKASLDDDSDIILLDVRTQEEYDELHIPGSILIPVNELEARANQELPDKD
                     AVIFIYCRSGNRSRTAAYKLLEMGYTQVYDIGGIIDWPYDVSSNKEK"
     gene            2317..2976
                     /locus_tag="PLI11_07630"
     CDS             2317..2976
                     /locus_tag="PLI11_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPZ52774.1"
                     /translation="MFDNYDLSRIVLFDGLDTDEIKTLWSCIDAKVKKYKRNDIIISE
                     GETVDAIGAVLCGSVQVSRTDIDGNRLVVASIQQYDLFAETYALSKNHESPVMVCATE
                     DSVVLWLDVSRLLTTCSSACIFHRRLIQNIIELLANKNVYLSKKLDILSKRTLREKIL
                     QFLHSYSTSSKERIITIPYNRLAMADYLGVDRSALSRELSKMKADGLIDYHKNTFILR
                     N"
BASE COUNT         1012 a          535 c          672 g          842 t
ORIGIN      
        1 acttttgcaa attacattta cttcttcagt tttgcattta cttttgcaat taaccaaatt
       61 ttcttgatga aattccatat atgtagtaaa attcatttgt ttagcattaa aaaatgtaat
      121 atcttgaaat aaattttatg gaggagaaga tatgataaaa tataaaggtc ctgtagtaac
      181 aataataatg gacggtattg gcctcaataa tactgtcgaa ggcaacgcag taaaaaacgc
      241 ctatacacca actctggaca gacttatgca aaattatccc atggtacaat tgcgtgctca
      301 tggtactgct gtaggcctgc cttcagatga agacatgggc aattccgagg ttggacacaa
      361 cgcaattggt gcaggcagcg tgtacagtca gggtgcaaag cttgtaaatg agtcaattga
      421 aagtggtaaa atgtttaaag gaaaaggcta tattgaaata cttgaaaatg tattgaagca
      481 caactcgact cttcatttta ttggcctttt ttctgacgga aacgttcatt cacatataga
      541 tcacttgaaa gcaatgataa aaagagcaaa gcaggatggt gttaaaagag taagagtaca
      601 cattcttctg gacggccgtg acgtgggtga gacatctgct cttgaatatg taattccttt
      661 tgaagaattt ctaagcggga ttaatggtcc tgattttgat gccagaattg ccagcggcgg
      721 tggaagaatg aacatcacca tggacagata tgaagccgat tgggcaatgg tggaaagagg
      781 ctggaaaatc catgttcttg gcgaaggcag gatgttttca agcagcgagg aagcaattaa
      841 gacatatcgc gaggagcttc atgtaattga tcaggatctt cccggatttg tcattgctga
      901 aaatggcgaa ccagttggca ggattgtgga taatgactct gttgttttct tcaacttcag
      961 aggcgacagg gcgatagaaa ttagcagagc ttttgaagag ccagacttcg acaagtttga
     1021 cagagtatat tttccaaagg tttgctatgc tggaatgttg gaatatgatg gcgatcttca
     1081 cattccttca agattcctgg tagaacctcc gcatatccag aacaccatgg gagaactttt
     1141 tgcaaagaaa ggaattaccc agcttgcaat aagtgaaacc caaaagtttg gacatgtgac
     1201 atacttctgg aatggaaaca gaagcggaaa attcgatgat gaacttgaaa catatgtaga
     1261 aattccatca gacatagttc cttttgagca aagaccatgg atgaaagcca gtgaaattac
     1321 cgacaggctc atcaaagagc tcagaacaaa caaatacaaa tatgcaaggg tgaactttgc
     1381 aaatggcgac atggtaggac atacaggaaa cttccttgct gcaaggattg ctgtagaaac
     1441 tgttgacctg tgccttgcaa gaatacttcc ggttattgat gagcttggag gcatggcaat
     1501 aattactgct gaccatggaa atgcagatga aatgtatgag gtgaagaaag gcaaattggt
     1561 tactgatgaa aacggaaaac caaaagcaaa aactgcccac actctgaatc ctgtgccctg
     1621 catcttctat gacaatacag agaacagaaa caagtacaaa gtaaaagaag gttttgataa
     1681 agatggcagg ataagatttg gtttatccaa tattgcagca acctgtgcct tcctattagg
     1741 agttgaaaaa cacgaatcct gggatgaaag cataattgaa taaataactg aaaaccgcca
     1801 attggcggtt ttctcgtttt ttaggtataa tatgctataa catttttaag gagtaattgc
     1861 tgtatgaaaa ataacacatt gcttttatca acaattttag ttgtcggtat tgcagttaca
     1921 accgttgtca tatggtacat attaagaagc ggaagttgtg aaagcgcagg aactgtgcaa
     1981 gtacgggaag gaaaatacat gaagcttgca ccctctgttg caaaggcaag ccttgatgat
     2041 gattccgaca ttattcttct cgatgttaga acgcaggagg agtatgacga gttgcatata
     2101 ccaggcagca ttctcattcc agtcaacgaa cttgaggcaa gagccaatca agagctgccg
     2161 gacaaggatg ctgtaatttt tatatattgc agaagcggta acagaagcag aacagccgcg
     2221 tataaacttt tagaaatggg atatacccag gtgtatgata taggcggaat tattgactgg
     2281 ccttatgacg tatcttcaaa caaggagaaa tagttcatgt ttgacaatta tgacttgtcc
     2341 aggatagtgt tgtttgacgg acttgacact gatgagataa aaacattgtg gtcctgcata
     2401 gacgcaaagg tgaaaaagta taaaaggaat gacattatta tatccgaggg tgaaactgtt
     2461 gatgctatag gagccgtttt gtgtggctct gtacaggttt cacgtactga tatagatggc
     2521 aacagacttg ttgttgcatc aatacagcaa tatgaccttt ttgcagaaac atatgctctt
     2581 tctaaaaacc acgagtctcc cgtcatggta tgtgcgacgg aagactcggt tgtcttgtgg
     2641 ctggatgtaa gcagattgtt aaccacatgc tcatctgctt gtatatttca caggaggctt
     2701 atacaaaaca ttatagagct tttagccaac aagaatgtat atctaagcaa aaaacttgac
     2761 atattgtcta aaagaaccct tcgagaaaag attcttcaat ttcttcactc ctactccacc
     2821 agcagcaagg aaaggattat cacaataccc tacaatcgcc ttgcaatggc ggactatctt
     2881 ggagttgaca gaagtgctct ctcaagagaa ttaagtaaaa tgaaagccga tggtttgatt
     2941 gactaccata aaaacacttt cattttacgt aactgagtct ttacttattc ttgctggaaa
     3001 tcaagaatac tacagtgaca actacagcca atgcagctat tacagctaca attataataa
     3061 c
//
LOCUS       DAONVY010000280         3054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_21994_length_3054_cov_2.921641, whole genome shotgun sequence.
ACCESSION   DAONVY010000280 DAONVY010000000
VERSION     DAONVY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21994_length_3054_cov_2.921641"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLI11_07635"
     CDS             complement(<1..269)
                     /locus_tag="PLI11_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52775.1"
                     /translation="MVSFIRTLIMYTFVIATIRVMGKRQIGELQPYELVIALMISDLA
                     ALPMQDTAIPLLSGIIPILSLLLAQLTISFVLLKSQKARNIICGK"
     gene            378..590
                     /locus_tag="PLI11_07640"
     CDS             378..590
                     /locus_tag="PLI11_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPZ52776.1"
                     /translation="MTQSMLDDKNLTVLEDQLNAEALAIKKYTLYKTQCTDPNLKSIC
                     EHAEQMHRRHFDSLYSYLQSHNKPMQ"
     gene            600..860
                     /locus_tag="PLI11_07645"
     CDS             600..860
                     /locus_tag="PLI11_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HPZ52777.1"
                     /translation="MLTEQEIMNDALMTEKQLISSYAVFLAETSCQNLRNEINKIITE
                     TGQVQFEIFNAMKSRGWYNVKNAQINDVQATYQKYTQVKNQL"
     gene            1052..2041
                     /gene="pta"
                     /locus_tag="PLI11_07650"
     CDS             1052..2041
                     /gene="pta"
                     /locus_tag="PLI11_07650"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPZ52778.1"
                     /translation="MNFLESICERAKKDLKTIVLPESEDIRTIKAAALVQERGIANII
                     LVGEEDKINELAEGLDISKCKIINPNTYERFEEYAQAFYELRKSKGITIEKARETMKD
                     YVYFATMMVKMGEADGMVSGAVHSTADTVRPALQILKTKPGVKMASAFFVMVVPDCEY
                     GANGVFVYADSGVVENPDASGLADIAIESANSFKQLVQEEPIVAMLSYSTYGSAKSEL
                     TEKVIEATRIAKEKAPELLLDGELQADAALVPDIGQKKAPGSKVAGYANVLIFPDLNC
                     GNIAYKLTQRLAKAEAYGPILQGLAKPVNDLSRGCSAEDIVGVVAITCVQAQK"
     gene            2060..>3054
                     /locus_tag="PLI11_07655"
     CDS             2060..>3054
                     /locus_tag="PLI11_07655"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPZ52779.1"
                     /translation="MKNILVINCGSSSLKYQLINAEDKVMLARGLCDRIGIDNSFIRQ
                     NVNGSKEYRKEVELPDHNAAINEVIKALTDKEYGVIESLEDIYAVGHRVVHGGEYFKS
                     SVVIDENVVKAIEKCIPLAPLHNPPNITGIKACQKAMPNVPQVAVFDTAFHQTMPKEA
                     YIYALPYEYYEKYSIRKYGFHGTSHQYVAERAAEMLGKPLEETRIITCHIGNGGSITA
                     IQGGKSIDTSMGFTPLDGLVMGTRSGNVDAAAVLYIMEEENLSCNEMNLILNKKSGLL
                     GVSGVSNDMRDVTEAKEKGNERAELAIKLFNYRVKSYIGQYMALMGGADAIVFTAG"
BASE COUNT         1047 a          514 c          673 g          820 t
ORIGIN      
        1 ttcttgccgc atattatgtt tcttgccttt tggcttttta ataatacaaa tgatatggta
       61 agctgagcaa gcaaaagaga cagtatagga attattccac taagaagagg tattgcagta
      121 tcctgcatcg gcagagctgc caagtcggaa atcataagcg ctattacaag ctcatagggt
      181 tgaagctctc ctatctgcct ttttcccata acccttattg tagcaatgac aaaagtatac
      241 attatcagag ttctgataaa gcttaccatt caatcattcc tttcaaatta gttctcctac
      301 atagtttaat ggaaaatgaa tattttattc cggacggtac agaatatata taaattcaat
      361 ttaaaaggag agaggaaatg actcaatcaa tgttggatga taagaacctg actgttctag
      421 aagatcagtt aaatgcagaa gctttggcaa tcaaaaagta caccctgtac aagactcaat
      481 gtacggatcc gaatctaaaa tccatatgcg aacatgctga acaaatgcat agaagacatt
      541 ttgattcgct ttacagttat ctgcaatccc acaataaacc aatgcaataa ggaggaaata
      601 tgttaacaga acaggaaata atgaatgatg ctcttatgac ggagaagcag ctgataagca
      661 gttatgctgt ttttttggct gaaacaagct gtcagaattt gaggaatgaa attaataaga
      721 ttataactga aacaggacaa gtgcaatttg aaatatttaa tgcaatgaaa tcaagaggtt
      781 ggtacaatgt caagaatgcg caaataaacg atgtgcaggc aacgtatcag aagtatacac
      841 aggttaagaa tcagttatag aggatagcac aaggagttgt tcccttgtgc tttctttgat
      901 ataatcgtct aggtgtgcaa atggctacat aaatattgtg tgaagttttg attaaattca
      961 ttgttaaagc acaaaatgta cttgcaaatt tttcattaaa gttataaaat cataaatggt
     1021 gtttttttag catatgaaag gtggaggaca gatgaatttt ttagaaagta tatgcgaacg
     1081 agcaaaaaaa gacttaaaaa ccattgtact tcctgaaagc gaagatatta gaactataaa
     1141 agctgcagcg cttgttcagg aaagaggaat agcaaatatc atacttgtcg gggaagaaga
     1201 taaaataaat gaattggcag aaggattgga tatatccaaa tgcaagatta taaaccccaa
     1261 tacttacgaa agattcgaag aatacgcaca agctttctac gaactcagaa aatccaaagg
     1321 cattacaatc gaaaaggcaa gagagacaat gaaggattac gtctactttg ccaccatgat
     1381 ggttaaaatg ggtgaagctg atggcatggt atccggagca gtccattcaa cagcagatac
     1441 agtaagacct gcgcttcaaa tcctgaaaac aaaaccaggt gttaaaatgg catctgcttt
     1501 ttttgtgatg gtcgttcctg attgcgagta cggagcaaat ggtgtatttg tatatgcgga
     1561 tagcggagtt gttgagaatc cagatgcaag cggacttgct gatattgcaa ttgaatctgc
     1621 caactccttc aagcaattgg ttcaggaaga acccattgtt gcaatgctgt catactcaac
     1681 ctatggcagt gcaaaaagcg aacttactga aaaagttatt gaagcaacaa gaattgccaa
     1741 ggagaaagca cctgagctgc ttcttgatgg tgaacttcag gcagacgctg ctttggtacc
     1801 tgatattggc caaaagaagg ctccaggcag caaggttgca ggttatgcga acgtattgat
     1861 tttccctgat ttgaattgtg gaaatattgc gtacaagctt actcaaagac ttgcaaaggc
     1921 tgaagcatac ggtccaatac ttcagggatt ggcaaaacca gttaatgacc tttcaagagg
     1981 ttgttcagct gaggacatag ttggtgttgt agcaataact tgcgtacaag cgcagaaata
     2041 aatatatcgg gaggcagtaa tgaaaaatat tttagttata aactgtggca gctcgtcgtt
     2101 gaaatatcaa ttgattaatg ctgaagacaa ggtaatgctg gcaagaggtt tgtgtgacag
     2161 aataggaatc gataactcct ttatacgcca gaatgttaat ggaagcaagg aatacagaaa
     2221 agaagttgag ttgcctgatc acaatgcagc aataaatgaa gtaattaaag ctctaacaga
     2281 taaagagtat ggagtaattg aatcattgga agatatatat gcagtaggtc acagggttgt
     2341 acatggagga gagtatttca agagctctgt tgttattgac gaaaacgtag tcaaagctat
     2401 agaaaagtgc attcctcttg caccactgca caatcctcca aatataaccg gaatcaaggc
     2461 ttgccagaag gctatgccaa atgttcccca ggttgcggta tttgatactg catttcatca
     2521 gacaatgcca aaggaagcat atatttacgc tttgccatat gaatattatg aaaagtattc
     2581 cattagaaaa tacggcttcc acggcacatc ccaccagtat gttgctgaaa gggctgctga
     2641 gatgctgggc aagcctcttg aggaaacaag aatcattaca tgccatatcg gcaatggcgg
     2701 cagcataaca gctatacagg gtggaaagtc aatagataca tccatgggat tcacacctct
     2761 tgacggcctt gttatgggaa ccagaagcgg aaatgtagat gccgctgcag ttctttacat
     2821 catggaggaa gagaatctct cctgtaatga aatgaacctt atacttaaca agaagtctgg
     2881 attgctcggt gtatctggtg tcagcaatga catgcgcgat gtaactgaag caaaggaaaa
     2941 aggcaatgaa agagccgagc ttgcaattaa gttgttcaac tacagggtca agtcatatat
     3001 aggtcagtat atggcattaa tgggcggtgc tgatgcgatt gtatttacag ctgg
//
LOCUS       DAONVY010000281         3025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22262_length_3025_cov_2.908754, whole genome shotgun sequence.
ACCESSION   DAONVY010000281 DAONVY010000000
VERSION     DAONVY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22262_length_3025_cov_2.908754"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2965)
                     /locus_tag="PLI11_07660"
     CDS             complement(<1..2965)
                     /locus_tag="PLI11_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HPZ52780.1"
                     /translation="MNLTGPSITVETACSSSLVAVHLACKAIMNGECNMAIAGGCSIS
                     LPKKSGYKYEDGMIKSPDGHCRAFDKDAKGTVGGDGIGLILIKKLENAVADRDHIYAV
                     IKGSAINNDGADKIGFTAPSVKGQARVIREAHRSSGVEPESIGYIEAHGTGTALGDPI
                     EIEALSEAFNTNKRNYCAVGSVKANIGHLDTAAGIAGLIKTVLALYYRIIPPSINFVE
                     PNPKINFERTPFYINTKVKKWGNKNYPLRAGVSSFGIGGTNAHVVLEEVPKDYATVNA
                     KTGCEQSRIKLIILSAKTKKALNRRIADLFDYMNNNDSSLEDIAYTLQVGRKSLDYKV
                     AFIVKDRVQLMNELYRVLNGGEPQISGCSSGNSKRIVFMFPGQGTQYVEMGKALYMSI
                     PSFKETVDKAFEITKKYTDIDMKGIWIPDSKKNNQDLQKDINQTEVAQVLLFIIEYSL
                     ALLLKNWGIIPDSMIGHSLGEYVAACVSGVLSLEDALKLVCCRAKFMQQMERGSMVSV
                     SLGKSEVEELLDGCSDVCIAATNSQKHTVISGTFKSVEKIIKIMEQRGISYIKLHVSH
                     AFHSFMMEPMLREYEKILKTVNFNTPTIPYISNLTGNFITVEQAKDPNYYLEHIRKTV
                     CFKEGIDTLSVYKDTIYLEIGPGGTLRRFVLENDCIGDTGCVVSVMKHAKEKEQDEIV
                     LLKAVASVWALGGTVDWCKFNKNSMGKRISLPEYPFEKEYYWKYGSLHAETKTTAKKM
                     SAVNKIDKWFYVPRWVKNNIKGTKEKQVYMVFDGTEQYSFETIDYLKSKGKTCIRVVQ
                     GKNFSCLGDDYYVIRPDSLDDYVQLLEQTLNNGNIPDVIVYMWSINNSEVGFEQYSEK
                     NIEYIKRNCFFNFVNIAKAIGIKGINTDIHMIAVTSNLERVFQHDEVDPFRALVKGAV
                     NVIPLEYKNISAFCIDVGRENWSLNKEMLFMDVVNLPDCKSIAYRNGERYVLSFRQMV
                     INRNIHNKDIFEEQEV"
BASE COUNT          882 a          668 c          404 g         1071 t
ORIGIN      
        1 atacttcctg ttcttcaaaa atatccttgt tatgaatatt tctattgatt accatctgtc
       61 taaaggatag tacataacgt tctccatttc tatatgctat acttttacaa tccggaagat
      121 ttactacatc catgaacagc atttccttgt ttaatgacca attctctcta ccaacatcaa
      181 tacaaaaagc cgatatattt ttatattcca gaggtattac attgaccgct cccttaacta
      241 aagctctaaa tgggtcaact tcatcgtgtt ggaacactct ttccaaattg cttgtaactg
      301 ctatcatgtg tatatccgta ttaatgcctt taattcctat tgcctttgca atattaacaa
      361 agttgaaaaa gcaatttcgc tttatatatt ctatgttctt ctctgaatac tgttcaaatc
      421 caacctcact attatttatg ctccacatat acacaattac gtcgggtata ttcccgttat
      481 tgagtgtttg ttctaataat tgtacataat catctagtga atcaggccta ataacataat
      541 agtcatctcc aaggcaggaa aaatttttcc cttgtacgac tcgtatacat gttttgcctt
      601 tactcttcaa atagtctatt gtttcaaaag aatactgttc tgttccgtca aaaaccatat
      661 aaacttgttt ttcttttgtc ccttttatat tgtttttaac ccatctaggc acataaaacc
      721 acttatctat tttatttaca gctgacattt ttttagcagt tgtttttgtt tctgcgtgta
      781 acgaaccata cttccaatag tactcctttt caaaaggata ctcaggtaac gaaattcttt
      841 ttcccataga atttttatta aatttacacc agtcaactgt cccaccaaga gcccatactg
      901 atgctacagc ctttaaaagt acaatttcat cttgttcctt ttcctttgca tgtttcatta
      961 ccgatacaac acatccagta tcaccaatac agtcgttctc caaaacaaat cttcgaagag
     1021 tccccccagg gccaatttct aaataaatag tatctttata tactgacagt gtatcaattc
     1081 cttccttaaa acatacagtc tttcttatat gttccagata gtagtttgga tctttggcct
     1141 gctccactgt aataaagttt cctgtgagat ttgatatgta tggtattgtg ggagtattaa
     1201 agtttacagt ctttaaaatc ttctcatatt cacgtaacat aggttccatc atgaaagaat
     1261 gaaaagcatg agatacatgc agtttgatat atgatatacc cctttgttcc ataatcttga
     1321 taattttttc cacactttta aaagtaccag atataactgt atgtttttga ctatttgtag
     1381 ctgcaataca tacatcactg catccatcca ataattcttc tacttcactt tttcccaaag
     1441 atacacttac catactacct ctttccatct gctgcataaa ctttgcgcgg caacatacca
     1501 atttaagagc atcttctaag gataaaacac cggaaacgca agcagcgaca tattccccaa
     1561 gactatgtcc aatcatgcta tcaggtatta ttccccaatt ctttaaaagc aacgcaagtg
     1621 agtattctat tataaaaagc agcacctgtg caacttcagt ctgatttata tccttctgca
     1681 aatcctgatt gttttttttc gagtcaggta tccatattcc tttcatatct atatcagtat
     1741 actttttggt aatttcaaat gctttgtcca cagtttcctt aaaagacggt atactcatat
     1801 atagtgcctt gcccatttcc acatactgtg ttccctgtcc cggaaacata aacactattc
     1861 tctttgaatt accggaactg catccgctta tttggggttc accaccgttt aacactctgt
     1921 ataattcatt catcaactgt actctgtctt ttacaataaa agctacctta taatcaagac
     1981 ttttgcgccc tacttgcaaa gtataagcaa tatcctccaa ggaactgtca ttattattca
     2041 tataatcaaa taaatccgct attcgtctat ttaaagcttt tttagtttta gccgacaata
     2101 taatcagctt tattctgctt tgttcacagc ctgtttttgc atttacagta gcataatcct
     2161 ttggtacttc ctcaagaaca acatgagcat ttgtgcctcc aattccaaat gagctgacac
     2221 cggctcttaa aggatagttt ttatttcccc actttttaac ttttgtatta atataaaagg
     2281 gtgtacgttc aaaattaatc ttcggattgg gttcaacaaa attgatacta ggtggaatta
     2341 tccgataata gagcgctaaa accgttttta tcaatccagc aatacctgct gcagtatcta
     2401 aatgtccaat atttgctttt accgaaccca cagcgcagta atttctctta ttagtattaa
     2461 aagcttcact caaagcttca atttctatag ggtctcccaa agcagttcct gtaccatgtg
     2521 cttctatata tccaatggac tctggctcaa caccgctaga tctatgtgcc tctctgatga
     2581 ctcttgcctg tccttttaca cttggtgcag taaatcctat tttatccgca ccatcattat
     2641 taatagctga tcctttaatt acagcgtata tatgatctct gtcagctaca gcattttcaa
     2701 gtttctttat tagtataaga cctatgccat ctcccccgac agtaccctta gcatccttat
     2761 caaaagcacg gcaatgtccg tcaggtgatt ttatcattcc atcttcatat ttgtaacctg
     2821 actttttggg taaagaaatt gagcatcctc cggcaatagc catattgcat tccccattca
     2881 ttatagcttt acatgccaga tgaaccgcta ccagtgaaga agaacatgct gtttcaacag
     2941 taatagacgg accagttaaa ttcattttat atgatatcgg catactcaac gagtgaaggt
     3001 tcatattacc tgattcccat ttttc
//
LOCUS       DAONVY010000282         3022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22288_length_3022_cov_2.597573, whole genome shotgun sequence.
ACCESSION   DAONVY010000282 DAONVY010000000
VERSION     DAONVY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22288_length_3022_cov_2.597573"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLI11_07665"
     CDS             <1..206
                     /locus_tag="PLI11_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52781.1"
                     /translation="LNNMVGMGIVSLPGMMTGQILSGTMPTTAILYQISIMIAICTVV
                     CLSVFGSLYLGYRTLYNERNQFL"
     gene            complement(325..1857)
                     /locus_tag="PLI11_07670"
     CDS             complement(325..1857)
                     /locus_tag="PLI11_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52782.1"
                     /translation="MIFKPRTGCMWDPTVMYINSKYYMFSMYKEKHTDLDNNMYMAVS
                     EDGVHWEDVGAVLEDTNGVCKMFVYETDEYVMVNFGSFSEESRANNDTLRFYRSKDLK
                     EWEYVGKATPDTRWYKGEGRYDHMYVIKEGDTYYGYPVATPKPEYKSCFGILTSKDGV
                     NWTSHKPPVIEWGDIPPINCLEGGGVEKINGKYYYIGGFVGYAGNYGYGLYTFIGDSP
                     MGPFKPDKEAFRLCGFDRLEGRVFVQNLACFARGDGQLLISNAVDAGGPYEIWLLPLR
                     KAHVDEDGHLRLAYWEQNDLLKGIEIKLDSDAFNLSFNTTKLYPSWRDKIFVPSNDGF
                     HACVEGSDDPVVNDRKMLVTIDRKLDLEKGFILEGKFAANSYPHYDEVNNKTQCWRPA
                     TFGVFMGEEDDKGMGIELEIGHPYKRKSYVTNVSISNNIMKNEIIDTIDEGCANVKGV
                     DANTTHTFKLLYRRNVYELYVNDLHVQTFVHLGKPNGTMGFILQNSDVTVSDIKLYEM
                     SL"
     gene            complement(1861..2664)
                     /locus_tag="PLI11_07675"
     CDS             complement(1861..2664)
                     /locus_tag="PLI11_07675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPZ52783.1"
                     /translation="MRVNVEDVIKAVKNAAKILTRDLGANESDMDIEIKASNRDVVTK
                     YDLEVQNSLIAELGSMYPDVLVIGEEDTKDSGQVGESKFAREFFLIDPVDGTTNFVHD
                     FRHSSISVAYGKDGKILLGVVMNPYTNEIFHAVKGKGAYLNNKRIHVSGHGIKDSLVL
                     FGTSPSTNDTHDRTFDILKHVFLNTKDIRRTGSAALDLGYIAAGRADFFFELILHPWD
                     YAAGIILIQEAGGIISDFNGNDLPLVRPSSVVAGNPLSYKDGIKLLKEV"
     gene            2808..>3022
                     /locus_tag="PLI11_07680"
     CDS             2808..>3022
                     /locus_tag="PLI11_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPZ52784.1"
                     /translation="MLQFVDTLREFSLLTVVIRLLLALLLGGLVGLERERNRQAAGLR
                     THILVCTAATMTMLIGIFAKEVIDLTT"
BASE COUNT          777 a          703 c          564 g          978 t
ORIGIN      
        1 cactaaacaa catggtagga atgggcattg tatccctccc tggaatgatg acaggtcaga
       61 ttctttcagg aacaatgccg acaactgcta ttctttatca gatatccatc atgattgcca
      121 tatgtacggt tgtatgcctt tcagttttcg gttctttata tctgggttac aggactctat
      181 acaatgagag aaaccagttt ctatgatttc ttttatttca gcattccttt gtaagtgata
      241 tgacataaaa aatccatcat cggttcaagc tgcatacttg aaatgatgat ggtttatttg
      301 tctttcttta tataactttt ctggttataa gctcatctcg tacagtttaa tgtcactaac
      361 agttacatcg gagttctgaa ggataaagcc cattgtacca ttgggtttgc caaggtgtac
      421 aaaggtttgg acatgcaggt cgtttacata cagctcgtaa acgtttcttc tgtacaagag
      481 tttgaaggta tgcgtagtat ttgcatctac tcccttaaca tttgcgcaac cttcatctat
      541 ggtatctatt atctcatttt tcattatgtt gttggatatt gaaacatttg taacatagga
      601 tttccttttg tatggatgcc ctatttcaag ttcaataccc attcctttat catcttcctc
      661 tcccatgaac actccaaaag ttgcaggcct ccaacattgg gtcttattgt tgacctcgtc
      721 atagtgagga taactgtttg ctgcaaactt gccttccagg ataaatccct tttccagatc
      781 cagcttgcgg tcaattgtaa cgagcatttt tctgtcgtta acgacaggat cgtcgctccc
      841 ttcaacgcat gcatgaaatc cgtcgttgct cggtacaaat attttatcac gccaagaagg
      901 ataaagtttt gtagtattga aagagagatt aaaggcatcg ctgtccaatt ttatttctat
      961 gcccttcaaa agatcatttt gctcccagta tgcaagtctc aaatgcccgt cctcatcaac
     1021 atgagctttc cttaagggca gcaaccaaat ctcatacgga cctcctgcat ccactgcatt
     1081 actgatgagt agctgaccat cgcctcgtgc aaagcatgcc aggttctgaa caaagaccct
     1141 gccttcaagc ctgtcaaagc cacacaacct gaaagcttcc ttgtcgggct tgaacggacc
     1201 cattggactg tcgcctataa aagtataaag tccgtagcca tagttgcctg catatcctac
     1261 aaatccacct atgtagtagt atttgccgtt tattttctca actccaccac cttcaaggca
     1321 gttgatggga ggtatgtcgc cccactctat cacgggaggt ttatggcttg tccagtttac
     1381 tccatccttg cttgtcagga ttccaaaaca ggacttgtat tcaggcttgg gggtagccac
     1441 tggatagcca taatatgtgt ctccctcttt aattacatac atatggtcgt atctgccttc
     1501 gcctttatac catcttgtgt caggggtggc cttgccaaca tattcccatt ctttaaggtc
     1561 ttttgacctg tagaatctca atgtgtcgtt gttggctctt gattcttcac tgaagctccc
     1621 aaagttaacc attacatact catctgtctc atagacaaac atcttgcaca caccgtttgt
     1681 gtcttcaagg actgcaccca catcctccca gtgtactcca tcttccgaca ctgccatgta
     1741 catattattg tccaaatccg tgtgtttttc cttgtacatg gagaacatgt agtatttgct
     1801 gtttatatac ataacagtag ggtcccacat gcaccccgtc cttggcttga atatcatggc
     1861 ctagacctcc ttgagaagtt tgattccatc tttgtaagat aaaggattgc ctgccactac
     1921 actggagggt ctgacaagag gaaggtcgtt tccgttaaag tcgcttataa tgccacctgc
     1981 ttcctggata agtattatgc ctgccgcata atcccaagga tgaagaatta attcaaaaaa
     2041 gaaatccgct cttcctgctg caatataacc aaggtcaagg gctgctgaac ctgttctcct
     2101 tatatcctta gtatttaaga atacatgttt cagaatatcg aaggttctgt catgggtatc
     2161 gttggtgctg ggagaagtac caaaaagtac aagggaatcc tttatgccat gcccgcttac
     2221 atgtattctc ttgttgttaa ggtatgcgcc ttttcccttc actgcatgga atatctcgtt
     2281 agtatagggg ttcatgacca caccaagcaa gatttttccg tctttgccgt atgcaacgga
     2341 gatgctgctg tgtctgaaat catgtacaaa gtttgtggtt ccgtccaccg gatctatgag
     2401 aaaaaactca cgcgcaaact ttgactctcc cacttgtccg ctgtcttttg tatcctcctc
     2461 tccaattacc aaaacgtcag gatacatgct tccaagctct gcaatcaagg aattctgcac
     2521 ttccaaatca tatttggtaa ccacatcccg gttgctcgcc tttatttcaa tatccatgtc
     2581 actctcattt gcacccaaat cccttgtaag gatttttgct gcatttttca ccgctttaat
     2641 tacatcctct acattaactc tcatacaatc ctcctgtttc acatagtcta ttcatatact
     2701 aatacagatt tgcaatttgg acaatccgtt ttaatcagac ccattttgtt ttcacacaaa
     2761 aatgtgataa aatacattat tatttattgc ataaaagggg tgatttgatg cttcagtttg
     2821 tggataccct tagagaattc agtttgctga ctgtggttat aaggcttctt ttggcgcttt
     2881 tgctaggtgg tcttgttggg cttgaaagag aaaggaaccg tcaggcggca ggcctgcgta
     2941 cccatatcct tgtctgcacg gcagcaacca tgactatgct tattggcata tttgccaagg
     3001 aagtcataga tctaactact ga
//
LOCUS       DAONVY010000283         3008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22413_length_3008_cov_3.651202, whole genome shotgun sequence.
ACCESSION   DAONVY010000283 DAONVY010000000
VERSION     DAONVY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22413_length_3008_cov_3.651202"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PLI11_07685"
     CDS             complement(<1..1273)
                     /locus_tag="PLI11_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPZ52785.1"
                     /translation="MKKIDSTILPRVKEQYMSDKYFTLGKKVTYYLDKNMADVGISSL
                     EIFLPELELTRVDTSNADIYFKYDASVSDKEEYYELHVGEKGIQIVYKDYLGARNASS
                     TIALLAEKDKDSYKVPVCTIKDYPDSMYRSGLLESSGRAWKDIDEIKEDLVRMALAKM
                     NYMNFHFFDYMGSSIRYDSYPMIKGFGKDNRQYTKDEIRDLVAFAKKLGIEIVPGIEM
                     PAHAQAILEAMPGLRCNGDIPNIWVLCAGNEMTYTVLDNIIKETAKLFPGKYIHIGAD
                     ELDFGDDTSWQPAWMGCSRCASLRQKEDLDSERDIFYYMIERIYASVKSHGKQMIMFN
                     DQIDISVPVDLPRDIIIHFWRIAIPGRGPVEGCSLERFLEEGFTVINSYYPETYVDFE
                     YYAKEETIRLWTPLSSPEFDKKYASQIMGGEF"
     gene            complement(1359..2153)
                     /locus_tag="PLI11_07690"
     CDS             complement(1359..2153)
                     /locus_tag="PLI11_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52786.1"
                     /translation="MKRMFLLVLLIICLSIVVMANERNLFVERDENLLGSGYFEDGVL
                     TNGRVETDSTYISVPGLTKNYTFKGRIRIVEMGSQPWNGVRIIIGANSSMETSKLVLT
                     KEWDVRVEFKNNNLNDLIGYFGAINEGMEIDFEVIREGQHFILKLNDNVCLEEDLPEE
                     LDAFEEGYEFNLGFESSDCHYEVTNIEIYCPDVPEETPFSTNTPEATTEATKTVEATS
                     TPTPSETDEPKEEKGISKVVVGLCVVAIVLVVLVVIVTVKDRKRKQ"
     gene            complement(2163..>3008)
                     /locus_tag="PLI11_07695"
     CDS             complement(2163..>3008)
                     /locus_tag="PLI11_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52787.1"
                     /translation="KAKGYSSDTNDYTSQGYGDNYYMQLVRNIRKYKGTPLDENYPKP
                     QTMKVDVYGEASAWDAITNTSNIYLNAAIENSPRDYKGASPSLHYTQDKADNFLKEIK
                     VTNDDSNLYFYIRTEGPIVDHDKEENIYLLLSVRGSKEKSWEGYHYIVNRKTESDKLT
                     ILEKVAKDDSFEFEEVGKVKYSVKDNIMQMEIPLALIGVSKDKIEIEFKVADSIEKPD
                     DIMDYYVSGSSAPIGRLNFTYLNMDETQKTLKTLNVLEIALGGIAVLVAAFSLGFLFN
                     RSRKK"
BASE COUNT          834 a          708 c          494 g          972 t
ORIGIN      
        1 aaaactctcc acccataatc tgggaggcat actttttatc aaactcagga gatgaaagag
       61 gcgtccataa cctgattgtt tcctccttgg catagtactc aaagtcgaca tatgtttccg
      121 ggtaatatga atttataact gtaaaacctt cttccaggaa acgctccaaa gagcatcctt
      181 ctacgggacc tcttccaggt attgcaattc tccagaagtg aataataatg tctcttggca
      241 gatcaacagg aaccgaaata tctatctggt cgttgaacat aatcatctgc ttgccatgac
      301 tcttaaccga agcatatatc ctctctatca tgtaatagaa gatatccctt tcagagtcaa
      361 gatcctcctt ttgccgcaat gaggcgcatc tcgagcagcc catccatgca ggttgccatg
      421 atgtatcgtc gccaaagtcc agctcgtcag caccaatatg aatatatttt cctgggaaca
      481 atttcgcagt ttccttgatg atgttgtcaa gaaccgtata tgtcatctca ttaccagcac
      541 acaaaaccca tatgttcggt atgtcaccat tgcacctcaa tccaggcatg gcttccagga
      601 ttgcctgggc atgagcaggc atctcaatgc ccggtactat ctcaataccc aacttcttgg
      661 caaatgcaac aaggtcccta atctcatcct ttgtgtactg cctgttatcc tttccaaagc
      721 cttttatcat gggataactg tcgtacctta tggaagagcc catgtaatca aagaagtgaa
      781 aattcatgta gttcatcttt gcaagagcca ttcgaacaag atcttccttt atttcgtcaa
      841 tatctttcca ggctcttccc gagctctcaa gaaggcctga tctgtacatg ctgtcaggat
      901 aatccttgat tgtacataca ggcactttgt atgagtcctt gtctttttca gccaaaagcg
      961 caatggtgga agatgcattt cttgcaccca aataatcttt gtatacaatc tgtatgccct
     1021 tttcaccgac atgaagttca tagtattctt ccttgtcaga aacactggca tcatacttga
     1081 aatatatgtc cgcattgctt gtatcaactc ttgtcaattc cagttccggc agaaaaatct
     1141 caagcgaact tatgcctaca tccgccatgt tcttgtcaag atagtacgta accttcttgc
     1201 caagagtaaa gtatttgtca gacatatatt gctctttgac tcttggcaat atggtagaat
     1261 caatcttctt catattagtt ttctcctcct ttttggtaca accaccaata atcataacag
     1321 ctacaatcaa aagaattgct atacgtttca tgtaaacctc actgtttcct ttttctgtcc
     1381 tttactgtca caattacaac aagaacaact agaacgattg caacaacaca caacccaacg
     1441 accaccttgc tgataccttt ttcctcttta ggttcatctg tttcagaagg agttggcgta
     1501 ctggttgctt ctacagtttt tgtagcctct gtggtagctt caggtgtgtt ggtggaaaag
     1561 ggtgtctcct ccggcacatc aggacaatat atctcaatat ttgttacttc ataatgacag
     1621 tcagaggatt caaagcccag attgaactca tagccttctt caaaagcatc aagttcctca
     1681 ggaagatctt cctccaaaca cacattgtca tttagcttaa gtataaaatg ctgtccttcc
     1741 ctgatcacct caaaatcgat ttccatccct tcgttaatag ccccgaaata tccaattaaa
     1801 tcattcaagt tgttgttttt aaactcgacc cttacatccc attccttggt caaaacaagt
     1861 tttgatgttt ccatggatga attggcccca attattattc ttacaccatt ccaaggttga
     1921 gaacccatct ctacaatcct tatccggcct ttgaatgtat agttttttgt aaggccagga
     1981 acagagatgt atgtagaatc cgtttcaacc ctaccgtttg taaggactcc gtcttcaaaa
     2041 taaccggaac ccaagagatt ttcatccctc tcaacaaaca gattcctctc atttgccatt
     2101 accacaatac tcaaacagat aattaataat acgagcaaaa acattctttt cataaaagca
     2161 cctcatttct tcctgcttct attgaataaa aatcccaaag aaaaggcagc taccaaaaca
     2221 gcaatgccac ccaatgcaat ttcaagcaca tttaacgttt ttaaagtttt ctgtgtttca
     2281 tccatattca aatatgtaaa attaagacga cctattggtg ctgacgagcc tgatacataa
     2341 tagtccataa tatcgtcagg tttttcaatg ctgtcagcca ctttgaactc aatttcaatc
     2401 ttatccttgc tgactcctat caaggcaaga ggtatctcca tttgcatgat attatccttg
     2461 acagagtatt ttaccttgcc tacttcttca aactcaaaag aatcatcttt tgccaccttc
     2521 tcaaggattg tcagtttgtc agactccgtc tttctgttga caatgtaatg atatccttcc
     2581 caggactttt cctttgaacc tctaacagat aataaaagat aaatgttttc ttccttgtca
     2641 tggtcaacaa ttggtccttc ggttcttatg tagaaataga ggtttgagtc atcgtttgta
     2701 acttttattt cctttaaaaa gttatcagcc ttgtcctggg tgtagtgaag acttgggctt
     2761 gctcctttgt agtctcttgg tgagttttca attgcagcat ttaaataaat gttgcttgta
     2821 ttggttattg catcccacgc ggaagcctct ccataaacat ccaccttcat ggtttgtggt
     2881 tttggataat tctcatccaa tggtgttccc ttgtacttcc ttatgtttct aacaagctgc
     2941 atatagtagt tgtcgccgta tccctggctt gtgtagtcat tcgtatcgga ggaataacct
     3001 tttgcctt
//
LOCUS       DAONVY010000284         3007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22419_length_3007_cov_2.952236, whole genome shotgun sequence.
ACCESSION   DAONVY010000284 DAONVY010000000
VERSION     DAONVY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22419_length_3007_cov_2.952236"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..870
                     /locus_tag="PLI11_07700"
     CDS             187..870
                     /locus_tag="PLI11_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ52788.1"
                     /translation="MRKLIVIMLVLATIFMIGCQNGDEKKDKPTATPVSSTPTPEPDP
                     TEEPTPVVTPEPTEGPKGVNVALDKPYEVSSFTINERRGWNPEFINDGEIECIDGYHY
                     GWTSQVGKYIGVDPSEAQEWVIIDLEKEYTIGTVIAWPRQDTGLYFPTDYQIDVSNDK
                     ENWTTVYEKKGDEGSAENDLSPRIIKLEDVKGRFVRFMGTLLRDDDQTHNDGLLMQLA
                     ELEIYSEDN"
     gene            978..1763
                     /locus_tag="PLI11_07705"
     CDS             978..1763
                     /locus_tag="PLI11_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPZ52789.1"
                     /translation="MLSELRNKKGFICDMDGVIYHGDRLLPGVKDFVDWLYKENKRFL
                     FLTNSSERSPKELQQKLYRMGLEVDESNFYTSALATAKFIASQSQGCSAYVIGAPGLF
                     NALYEAGITMNNVNPDYVIVGETSNYHYENLVLACRFVLNGAKLIGTNTDITGPSESG
                     LVPACRSLIAPIEIATGKKAYFVGKPNPLMMRTGLKMLGVHSAEAVMIGDRMDTDIIA
                     GIETGLETVLVLSGVTSREELDLFPFRPGYILNGVGDIVNGKI"
     gene            1795..2733
                     /locus_tag="PLI11_07710"
     CDS             1795..2733
                     /locus_tag="PLI11_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPZ52790.1"
                     /translation="MRKYLLPKDGIFYKANLHCHSTHSDGKLTVEQLKEVYKKEGYSV
                     VAFSDHNVLIPHMDLCDEEFLPITSIEIDVSDNTVPWPKNATYHINFFSKDPYADKFI
                     ELERIYNADVINDIIRRAKERGFIAQYNHPRWSLQTACDFLPLEGLFAFEVYNTGCEV
                     DMMNGWGEYEYETYVMSGKRCGCVATDDNHNSQELKSPKSDSFKGFTMIKAKELSYDS
                     VMNALEDLDFYASTGPVIHELYVEDNTVYIETSPCSCILLRTDSRISKSTREESDVLT
                     KHAFSLDFNYEYIRFECSDIFGNKALTRAYFKDEIQ"
     gene            complement(2714..>3007)
                     /locus_tag="PLI11_07715"
     CDS             complement(2714..>3007)
                     /locus_tag="PLI11_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPZ52791.1"
                     /translation="MDTVWEVNPDCEFVLVATMLANGEVANFLGNQEEYLPALNTLKG
                     NMVAIADMTTMHKYLLKRKRYYDMSGNNVNHPNDFLARIYAQVMAATLIESRL"
BASE COUNT          928 a          545 c          692 g          842 t
ORIGIN      
        1 aaactttgtt tcaattgaaa aaaggactct tccaaatgtg cagaatgcag cacagaacag
       61 gttcgaattt gattctgtca atgcgaccaa gatcaagatt aaaatcacat cttcatattc
      121 ctcaaagaat gtgcagattg cagaggttaa ggtgtataaa agtgagtaaa gaactggagg
      181 tagaatatga gaaagctaat tgttatcatg cttgtactgg caacaatatt tatgattggc
      241 tgtcagaacg gtgacgaaaa gaaggataaa ccaactgcca cacctgtatc gagcacacct
      301 actcctgaac cggatccaac ggaagagcca acccctgtgg tgactccgga gccgacagaa
      361 ggtccaaaag gagtcaatgt agctctcgac aagccatatg aagtttcttc atttacaatt
      421 aatgagagaa gaggatggaa tccagaattt atcaatgatg gagaaataga gtgtattgat
      481 ggctatcatt atggctggac cagtcaggtt ggtaaatata ttggtgttga tccatcagaa
      541 gcgcaggaat gggttatcat tgacctggaa aaagagtaca caatcggtac tgttattgca
      601 tggcctcgtc aggacacagg tctgtatttc ccaacggatt atcagatcga tgtttctaac
      661 gacaaggaga actggacaac agtatatgag aaaaaaggcg acgagggttc tgctgaaaac
      721 gatctgtctc caaggattat aaagcttgaa gatgtaaagg gtagatttgt aaggttcatg
      781 ggaacactat tgagagacga tgatcaaacc cacaatgacg gcttgcttat gcagcttgcc
      841 gaactggaaa tctactcgga agacaattaa agaaaaactg tttgggtgcc atttggaaag
      901 tggcaccttc tttttttgtc tttaatgtag tatacttgac tggaagaata ataaaggttg
      961 gaggaatttc ttaacgtatg ttgtctgaac ttagaaacaa aaaaggcttt atatgcgaca
     1021 tggatggtgt aatatatcat ggagacaggc ttttaccggg tgtaaaagat tttgttgact
     1081 ggctgtacaa ggaaaacaaa agatttcttt tccttactaa ttcaagtgag aggtctccga
     1141 aggaacttca gcagaaactc tacagaatgg gtcttgaagt ggatgaaagc aacttctata
     1201 caagtgcgct tgctacagca aaatttatag cgagccagtc acaaggctgc agtgcttatg
     1261 ttataggggc gcctggtctg ttcaatgctc tttatgaagc tggcattaca atgaataatg
     1321 ttaacccaga ttacgtgatt gttggagaga caagcaacta tcactatgaa aatttggttc
     1381 ttgcatgcag gtttgtatta aatggagcaa aactcatagg taccaatacc gatattacag
     1441 gaccatctga gtcggggctt gtacctgcct gcagatcctt gattgctcca attgaaattg
     1501 ctacaggcaa gaaagcatac tttgtaggaa agccgaatcc tttaatgatg cgtactggtc
     1561 ttaagatgct gggtgtacac tccgctgaag ctgttatgat tggtgacaga atggatacgg
     1621 acattattgc cggtattgaa actggccttg agaccgtgct ggttctttcc ggagtaacct
     1681 caagagaaga attagatttg ttccccttca gaccaggata tattttgaat ggtgtggggg
     1741 acatcgttaa tggaaaaatt taatagatta aattaagttt tggaggcgct ttttatgaga
     1801 aagtatctgc tgccaaagga tggtatcttt tacaaggcaa atctgcactg ccacagcacg
     1861 cattccgacg gaaagctgac tgtggaacag ctgaaggaag tatataaaaa ggaagggtac
     1921 agcgtagtcg cgttttctga ccacaatgtt cttataccac acatggattt gtgcgatgag
     1981 gaattcctgc ccataacttc aattgaaata gatgtgtcgg acaatacggt tccatggcct
     2041 aaaaatgcca catatcacat aaatttcttc tcaaaggatc cttatgctga caagttcatt
     2101 gagcttgaaa gaatttataa tgcagatgtt attaacgaca tcataagaag ggcaaaggaa
     2161 agaggtttta tagcccagta caaccatccc agatggtcgc tgcagacagc ttgcgatttt
     2221 cttcctcttg aaggcttgtt tgcctttgag gtatacaata caggctgtga agtggatatg
     2281 atgaatggct ggggcgaata tgagtatgaa acctatgtaa tgagtggaaa gagatgtggt
     2341 tgtgtggcta ccgatgacaa ccacaactcg caagaactga agtcacccaa aagtgattca
     2401 ttcaaaggtt ttacgatgat aaaggcaaaa gaactttctt atgattctgt tatgaatgca
     2461 ttggaagatc ttgattttta cgcctccacc ggacctgtaa tccacgaatt gtatgttgag
     2521 gacaatacag tgtatattga aacatctccc tgctcatgca ttctcctccg cacggattcc
     2581 agaatttcaa agagtacaag ggaggagtcg gatgttctga caaaacatgc attttctttg
     2641 gatttcaact acgaatacat ccgatttgaa tgttcggaca tatttggaaa caaggcgctg
     2701 acaagagcgt actttaaaga cgagattcaa taagtgttgc tgccataact tgtgcataaa
     2761 ttctggcaag aaaatcattg ggatgattta cattgtttcc ggacatgtcg tagtatctct
     2821 ttcttttaag caggtatttg tgcattgtgg tcatgtcggc tattgccacc atgtttcctt
     2881 taagcgtatt cagtgcaggc aggtattctt cctggttgcc aaggaagttt gccacctctc
     2941 catttgccag catggttgca acaagtacaa actcacaatc cggattgact tcccaaacag
     3001 tatccat
//
LOCUS       DAONVY010000285         3006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22432_length_3006_cov_2.850898, whole genome shotgun sequence.
ACCESSION   DAONVY010000285 DAONVY010000000
VERSION     DAONVY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22432_length_3006_cov_2.850898"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLI11_07720"
     CDS             <1..240
                     /locus_tag="PLI11_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPZ52792.1"
                     /translation="NQINNVLAFPGIFRGALDVRARDINDEMKIAAAKALADLIPENE
                     LNPDYVIPDPFNKDVAKVVAEAVAQAARDTGVARI"
     gene            237..1637
                     /locus_tag="PLI11_07725"
     CDS             237..1637
                     /locus_tag="PLI11_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPZ52793.1"
                     /translation="MMPIVDMLVQHAKKEKISMHMPGHKAGMLFSYSHPFLTEEYSSF
                     LRNLAEVAKHDVTETLGVDSLHDPKGAILKAMELTASTYGAASSFFLVNGSTCGVHAM
                     IYSSFRRGDTVFVSSDCHMSVFNALMVTGCNVVVVPVKVDEDTLIPLAITPEDVRKAY
                     EMFPDSKGIVMTRPNYYGVCADLAAISEIIHENKGVVLVDEAHGGHFGFNKKIPESAL
                     SQGCDMCVQSAHKTLQSLNQGAFLHIADNKYKDKVFRALKVIETTSPSFLILATLDFT
                     REYMKNMGRERLDELADSLNDFVSRLEKYEDYIIFDGCGFEKDITRLVVCVKGKGVHF
                     ARMLADRGVYCEMYNDNCVVFILTCADDKDVIERLFDTLVSVYDELSKVEVEEKSIRV
                     YKGEIREINLPNFETYMNLEYETVDVKEAVGRISSQFVIPYPPGIPVIFPYDRINDAH
                     ADICRSYCIERINVIK"
     gene            1667..2002
                     /locus_tag="PLI11_07730"
     CDS             1667..2002
                     /locus_tag="PLI11_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HPZ52794.1"
                     /translation="MKLVLAIVHDDDAPRVMDELNKNGFMVTKMCSSGGFLKSGNTTL
                     LVGVDESRVDQVLQIIESKSRSRKQIIDSSVTNSSAIGGGVYMSYPVEVTVGGATVFV
                     LDVERFQKY"
     gene            2061..>3006
                     /locus_tag="PLI11_07735"
     CDS             2061..>3006
                     /locus_tag="PLI11_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HPZ52795.1"
                     /translation="MKRTLLDIAESGRIGHAYLFVGPEGIGRKSIAKRFAQLVMCMGE
                     TTNRGEVCGVCTSCKLHGSDTNPDYKIISEPEGKNAIGIDAIREIQESMTRAPLYGRR
                     SFYVINNAEKMTVQAQNALLKTLEEPPEYVVLVLVCQNVSLLLETVKSRLSRMDFSRN
                     TDEEIGEIFKRNGVESNDMIYSYADGIPGRALSLIDSDVEVLRKDIINSVVKVTSGGV
                     AERKQAMKVIEGYQDKRDFVFYTFMSIYRDIMIMSRYARNVKIQNAGYDKELYSLARK
                     LGFHKAKECIEVVDSTWKRIGKNVNFKLSCELMLVKLQE"
BASE COUNT          922 a          482 c          777 g          825 t
ORIGIN      
        1 aaccagatta acaatgtcct tgcattccca ggcattttca gaggtgcgct tgatgtaagg
       61 gcacgtgata taaatgatga aatgaagatt gcagcagcaa aggctttggc ggatttgatt
      121 cctgaaaatg agcttaatcc cgattacgtg atacccgatc ctttcaacaa ggatgtggca
      181 aaggttgttg cggaagcggt ggcacaagct gcaagagata caggtgtagc aagaatatga
      241 tgccaatagt tgacatgctt gttcaacacg cgaaaaagga gaaaatatca atgcacatgc
      301 ccggtcataa agcgggcatg cttttttctt attcccatcc tttcctgaca gaggaatact
      361 cgtccttttt aagaaatctt gcggaagttg caaaacatga tgtaactgaa acattaggag
      421 ttgacagtct gcatgacccc aaaggtgcaa tattaaaggc gatggaactt acagcgtcaa
      481 cctatggtgc tgcatcttcc ttctttttgg tgaatggttc cacctgcggt gttcatgcga
      541 tgatttattc atccttcaga aggggggata cggtttttgt atcttctgac tgtcatatgt
      601 ccgtattcaa tgctttaatg gtaactggct gcaatgtggt tgttgtgcct gttaaagtgg
      661 atgaggatac attgattcca ctggcaatta caccggagga tgtaagaaaa gcgtatgaaa
      721 tgtttcctga ttcaaagggg attgttatga caagacccaa ctattacgga gtctgtgcag
      781 acctggcagc catatcagaa atcatacatg agaataaagg cgtggttctt gtggatgaag
      841 cccacggtgg tcattttggt tttaacaaga aaattcctga aagtgcattg agccaggggt
      901 gcgacatgtg tgtgcagagt gcccataaaa cgttgcagtc attgaatcag ggtgcgttct
      961 tgcatattgc agacaacaag tacaaggata aggttttcag ggcgttaaag gttatagaaa
     1021 cgacaagccc gagcttttta atactggcca cgctggattt cacaagagag tatatgaaaa
     1081 atatgggcag agagagactt gatgagcttg cagacagcct caatgacttt gtttcacgtc
     1141 ttgagaagta tgaggattac ataatatttg acggctgtgg ctttgaaaag gacattacaa
     1201 ggcttgttgt atgtgtaaaa gggaagggcg tccattttgc aagaatgctg gctgacaggg
     1261 gagtatattg cgagatgtac aatgacaact gcgttgtttt catattgaca tgcgcagatg
     1321 ataaagatgt tattgaaagg ctttttgata ctcttgtgtc agtctatgac gaactgtcaa
     1381 aggtagaggt tgaagagaag agtataaggg tgtacaaggg cgagattagg gagataaatc
     1441 ttcccaactt tgaaacatac atgaatcttg agtatgagac tgttgatgtt aaggaagccg
     1501 tcggcaggat aagcagtcag tttgttatac catatcctcc cggaataccg gtaattttcc
     1561 catatgacag aataaacgat gcccatgccg atatatgtcg gagttattgt attgaaagga
     1621 taaatgtgat aaaatagagc taatccaatg ttaggagggc aagcttatga aactggtatt
     1681 ggcgattgtg cacgatgacg atgcaccaag agtaatggat gaactaaata aaaatggttt
     1741 tatggtaacc aaaatgtgtt catcaggtgg attcttgaag tcaggaaata ccacactgct
     1801 ggttggtgtt gacgagtccc gcgtggatca ggtgctgcag ataattgagt caaaatcaag
     1861 aagtcgaaaa cagattatag attcttcagt caccaatagt tccgccattg gaggaggggt
     1921 atacatgtca tacccggtag aagtaactgt aggcggagcg acagtatttg tacttgatgt
     1981 tgaaagattt cagaagtatt aatggaggca ttatttaaag atatatcatg gtttttgaag
     2041 atatagcagg gcaggataaa gtgaaaagga ccttgctcga tattgcagag tcgggcagga
     2101 taggacatgc ctatctgttt gttggtcctg aagggattgg tcgtaaaagc atcgcaaaaa
     2161 ggtttgccca gctggttatg tgcatgggag aaacaacaaa tagaggcgaa gtatgcggtg
     2221 tttgcaccag ctgtaaattg catggaagcg atacgaatcc ggactacaaa attatatccg
     2281 agcctgaggg caaaaatgct attggaatag atgcgatacg agaaatacag gaatcgatga
     2341 caagagctcc tctttatggg aggaggagtt tttatgtgat aaataatgcg gagaaaatga
     2401 ctgtacaggc gcagaatgct cttttaaaaa cattggagga accgccggaa tatgtagtgc
     2461 ttgtgcttgt ttgccagaat gtgtcccttt tgttggagac ggttaaatca agattgagca
     2521 gaatggattt ttccaggaat actgacgaag agataggaga aatatttaag agaaacggtg
     2581 tggaatctaa cgacatgata tattcttatg cggacggaat tcccggacgt gctctatcgc
     2641 ttattgattc tgatgtggaa gtgttaagaa aggatatcat aaatagtgtt gttaaggtta
     2701 caagcggcgg tgtcgctgaa agaaagcaag cgatgaaggt tattgaggga tatcaggata
     2761 agagggattt tgtattttat acctttatgt ctatatacag ggacataatg attatgtcca
     2821 gatatgcacg gaatgtgaaa atacaaaatg caggttacga taaggagctt tacagccttg
     2881 cacgcaaatt gggctttcat aaagccaagg agtgcattga agttgttgat tccacgtgga
     2941 aacgcatagg aaagaatgtc aacttcaagc tgtcttgcga acttatgctg gttaaactac
     3001 aggagg
//
LOCUS       DAONVY010000286         3000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22487_length_3000_cov_3.296774, whole genome shotgun sequence.
ACCESSION   DAONVY010000286 DAONVY010000000
VERSION     DAONVY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22487_length_3000_cov_3.296774"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..556
                     /locus_tag="PLI11_07740"
     CDS             176..556
                     /locus_tag="PLI11_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPZ52796.1"
                     /translation="MDISISNTSEMPIYEQIYNQIKAQIMQGRLLEGEALPSIRSLAK
                     DLRISVITTKRAYDELEKDGFIYTVLGKGCFVSKRNVELIREAHLKEIEEFMKKIIDL
                     ASVIDLTKEEIIEMFNILYEVDKE"
     gene            553..1410
                     /locus_tag="PLI11_07745"
     CDS             553..1410
                     /locus_tag="PLI11_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52797.1"
                     /translation="MNAIEVRNLVKKYPGFKLDNVSFQLPKGYIMGLVGDNGAGKSTT
                     MKLIMNSIKRDSGVVKVLGCDNTSKSFVKVKEEIGVVLDEALFPEVLNAKQVNNIMKR
                     TYMNWEEDRFFGYMDRFSLPPDKVFKDYSRGMKMKLSISVALSHKSRLLVLDEPTSGL
                     DPIVRDEILDILNEFTRDEENSIVISSHITGDLEKICDYVAFLHKGKLVLCEEKDTLL
                     YKYGIVHATRSEFGVVPKNAIVSKRETPYGIDALVFREKTPKTVKIEKPTIEDIFVFM
                     HKYREENYK"
     gene            1407..2012
                     /locus_tag="PLI11_07750"
     CDS             1407..2012
                     /locus_tag="PLI11_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPZ52798.1"
                     /translation="MKGLLLKDFYTLIKQVRFFLLVLLFMALLPGTSMAPFVILYAVM
                     LPVSILGYDERAKWDRYAAMLPYTKKSIVLSKYIMGYAGLILASLLTVTAHVVYANIK
                     GSVPELGYFYEIGVLVCIALFYLAVHLPFMYKFGVEKGRLVFLALTAVVVFGLMYLSD
                     KISKGLMLDPYDLLAYIIPVAITANLVSILVSIRIYEKKEF"
     gene            2016..2405
                     /gene="cdd"
                     /locus_tag="PLI11_07755"
     CDS             2016..2405
                     /gene="cdd"
                     /locus_tag="PLI11_07755"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPZ52799.1"
                     /translation="MNVRDLIKLAFSAKEKAYSPYSNFKVGAALETESDVVYTGCNIE
                     NAAYTPTNCAERTAFFKAVSEGVYYFKRIVVVGGDNEFCYPCGVCRQVMQEFCDENEF
                     EIIVAKDEENYEVYKLKELLPKGFKLE"
     gene            2563..>3000
                     /locus_tag="PLI11_07760"
     CDS             2563..>3000
                     /locus_tag="PLI11_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52800.1"
                     /translation="MCRRKVVLLFACLLVFGLGILITNADSSESKWVSLPYVAQGDEG
                     NIDYLYAVGGYHSIDSGTDIWGTASYSEIRLVGDRMGDIVITYESGTVDKVPLIFGYT
                     LWYYNNWKEYKAPFDGEAKNEKMANLLKEALHLNGAFEGHPPHV"
BASE COUNT          905 a          446 c          719 g          930 t
ORIGIN      
        1 cgggctttct ttgaaaatat tttgacttct tcatttttat cccccacagc cttgcctgtt
       61 ctatttatat gtcttttatt caggaaagtt gaattttatg cttgacatgt tttttatagt
      121 tgtatatact gattatactg tatatatacg gtatatacag ttgaggaggg aagaaatgga
      181 catatcaata agtaatacta gtgaaatgcc catatatgag cagatataca accaaataaa
      241 ggcacagata atgcagggca gacttcttga aggggaggct ttgccgtcca tccgctccct
      301 ggccaaggat ttgcgcataa gtgttattac tacgaagcgg gcgtatgatg aacttgaaaa
      361 agacggcttt atttacacgg ttcttggcaa aggctgcttt gtatcgaaaa ggaatgtgga
      421 acttattcgt gaagcacatt tgaaggaaat agaggagttt atgaaaaaga taattgatct
      481 tgcctccgta attgatttga caaaggagga gataattgaa atgtttaata ttctgtacga
      541 ggtggataag gaatgaatgc cattgaggtt agaaatttgg tgaaaaaata tccgggtttt
      601 aaactggaca atgtatcttt ccagctgccg aaaggctata tcatgggcct tgttggagac
      661 aacggtgcag gaaagagtac taccatgaaa ctcataatga attcaattaa aagagactcg
      721 ggagttgtta aggttttggg ttgtgataat acaagcaaaa gctttgtaaa agtcaaggaa
      781 gagattggcg ttgtccttga tgaggcgtta tttccggaag tcttgaatgc aaaacaggta
      841 aataatatta tgaaacgtac ctacatgaat tgggaagagg atagattctt cggttatatg
      901 gatagatttt cgctgcctcc tgacaaagtt ttcaaggatt actccagagg tatgaagatg
      961 aagctgtcaa ttagcgtggc cttatcacat aagagcaggc ttttggtact cgacgagcca
     1021 accagtggcc ttgatcctat cgtaagagac gagatactcg atattttaaa tgagtttaca
     1081 agggatgagg aaaacagtat tgtaatatct tcccacataa ccggtgactt ggagaagatt
     1141 tgtgactatg tggcttttct ccacaaaggc aaacttgttt tatgtgaaga aaaggatacg
     1201 cttttataca aatatgggat tgtacacgca acaagaagcg agttcggggt tgtacctaag
     1261 aatgcgattg taagtaaaag ggagactcca tatgggattg atgctcttgt gtttagggag
     1321 aaaaccccca agacagtcaa gattgaaaaa cctacgattg aggacatatt tgtatttatg
     1381 cacaaataca gggaggagaa ttacaagtga aagggttgct tttaaaagat ttttatactt
     1441 tgattaaaca agtcagattc tttctgttag tgcttttgtt tatggcactg cttcctggaa
     1501 catcaatggc tccatttgtc attttgtatg cagtaatgct gcctgtatcg attctgggat
     1561 atgatgaaag agccaaatgg gacagatatg cagcaatgct tccatataca aagaaaagta
     1621 ttgtacttag taaatacatt atgggttatg cggggctgat cctggcttct ttactgacag
     1681 taactgctca tgttgtctat gccaacatta aaggtagtgt gcctgagctt ggatattttt
     1741 atgaaattgg cgtgcttgtg tgcattgctc ttttttatct ggctgttcat ttgccgttta
     1801 tgtacaagtt cggagttgaa aagggaagac tggtttttct tgcactgact gctgttgtgg
     1861 tttttggctt aatgtatctt tctgacaaga tttcaaaggg cttgatgctt gacccttatg
     1921 acttacttgc gtatattata cctgtggcta ttacagcgaa ccttgtatcc atacttgtat
     1981 ccataaggat ttatgagaaa aaggagtttt gagcaatgaa tgtacgggat ttgataaaac
     2041 ttgccttcag tgcaaaggaa aaagcttatt caccttattc gaatttcaag gtgggagcag
     2101 ccctggaaac agaaagtgat gtggtttata cggggtgtaa tattgaaaat gcagcataca
     2161 cacctacaaa ctgtgctgaa agaactgctt ttttcaaagc tgtgtcagaa ggtgtttatt
     2221 acttcaaaag gattgttgta gtaggtgggg acaatgagtt ttgttatcct tgtggtgtgt
     2281 gcaggcaggt aatgcaggaa ttttgtgatg agaatgagtt tgaaattatt gttgcaaagg
     2341 atgaggaaaa ctatgaagtg tacaaattaa aagagctgct gccaaaaggg ttcaagcttg
     2401 aatgaaaagg cagttagggt gcagtggatg cacctctgtt ttttttgccc gatatgttaa
     2461 cagaatgtga aaaacatgtc tgaattgtat atgtaattac atgcaatcaa ctgtatatta
     2521 aggtaaaata atatatttgg cgtatagaat cgggaggaaa acatgtgtag aaggaaggtt
     2581 gtgctgttat ttgcgtgctt gttggtattt ggtttgggaa ttctaattac caatgccgat
     2641 tcgtcggaaa gcaagtgggt aagtctgcca tatgttgcac aaggagacga aggaaacatt
     2701 gattatttgt atgctgtagg tggctatcac tccattgaca gcggaacgga tatctggggt
     2761 actgccagct acagtgaaat aaggcttgtt ggtgatcgaa tgggtgatat cgttattact
     2821 tatgaaagtg gaacggtgga caaggttcct ttgatttttg gatacaccct ttggtattac
     2881 aacaattgga aggaatacaa agctcctttt gatggtgagg caaagaatga aaaaatggca
     2941 aaccttctaa aggaagcttt acatcttaac ggtgcctttg aaggacatcc tccacatgtg
//
LOCUS       DAONVY010000287         2998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22505_length_2998_cov_3.224261, whole genome shotgun sequence.
ACCESSION   DAONVY010000287 DAONVY010000000
VERSION     DAONVY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22505_length_2998_cov_3.224261"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1199)
                     /locus_tag="PLI11_07765"
     CDS             complement(213..1199)
                     /locus_tag="PLI11_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52801.1"
                     /translation="MRYFVGVDQGGSKTEILLGDENGLIVDKLSGFGYSAFIQDIKDS
                     SEFAVRYLGQQVDYLKDLLQRNGIEMPDVYALTACMATVDSDDIKKYYEDNLRLRTSI
                     ENIKVYNDMYGAWRAGTNRLPSGVLGVGTGAGIWLFDENGKAFNLSGRINYQAALGLG
                     YRAFLHACMSAQYLIEPTILSERICEFAQTPTIAEALKKTNNGWDVNALPYQHFVPFV
                     YDAMLLKDRVAEDFVNQTGKGFADCIREGIRELGWENREVHFVMNGGSFKGKGFVLEK
                     VIREYLSDLDNLILFQSEYEPVAGALLLSYDNYGISPDYHSFMRFKLRRNLH"
     gene            1341..2957
                     /locus_tag="PLI11_07770"
     CDS             1341..2957
                     /locus_tag="PLI11_07770"
                     /inference="COORDINATES: protein motif:HMM:NF037720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPZ52802.1"
                     /translation="MNKIKGVVMGRFKHFSVLIECSAGNTPKVDTIKHFIRMLSRMGY
                     NELYLGCTDAYKMEGEPYFNYKRGGYTTEDFQEMDACAKENGIELIASIQTLGHLHFL
                     QRHESYRDLMDNDHVLLVGDERVYKLIDKMFATISKGLSTKRIHIGYDECWGLGLGNY
                     LKKNPPADKKELLLRHLSKVTNIASKYGYTCEVWHDMLTETLNTKVTAEQVRNALPEN
                     TTMFYWNYFEGDVHVLRSGLDNMFKYTDKVGYAGSVFKCGSMAPMNTFSLSRLLTQMR
                     VAEEKGLDHFMITLWSDNGAWVNNYTVLPALYACAEFNIGRWDGTGELDKARFKDITG
                     ADYDDMLSLDYLDDPYKKKLTNTHANLTFLIFFADLLNGGWDLLYDKDMPKTYEDLAN
                     HYQRLACGDYSHVFKCAEKLARVLSIKCLLGLTIRNAYKANDTKTLKESVERLKELEN
                     AIIEFSKAYADMWLHDSMPYGLEVCQTLIGGQMLRTSYVKQVIEAYLKSGRPIPELAC
                     ETIVPDLPPGTTQDSCWIADWKLLISNCGF"
BASE COUNT          950 a          640 c          530 g          878 t
ORIGIN      
        1 gacatccaga caatgctgca aaacaatata gtaatatata gattaggttt aattgcaaat
       61 ttcactctct tatacgttca atttaaagca tgactatatt ctattacctt tttatcttta
      121 catgcttact cttgtcaagt ttgttaactt gtcaaagttt ttcatctctc tttaaatatt
      181 tgccatttct tttatcttgc gcattacctt tatcaatgta agtttctcct tagtttaaac
      241 cgcataaaac tatgataatc aggactgata ccgtaattat catacgaaag cagcaatgct
      301 ccagcaacag gctcatactc ggattgaaaa agtatcaaat tatcaagatc agaaagatat
      361 tctctaataa ctttttcaag aacgaaacct ttacctttga atgaacctcc attcattaca
      421 aaatgtactt ccctgttctc ccatcccagc tctcttattc cttcacgtat acaatcagca
      481 aaacccttgc ccgtctggtt tacaaaatcc tctgcaactc tatcctttaa taacatggca
      541 tcatatacaa aaggcacaaa atgttgataa ggaagtgcat taacatccca cccattattt
      601 gtctttttca acgcttcagc tatagtcggg gtttgtgcaa actcgcatat tctctctgaa
      661 agtattgttg gctcaatcaa atactgtgca gacatgcatg catgtaaaaa agcacgataa
      721 ccaagtccta gtgcagcttg ataattaatt cgtcctgaca aattaaacgc ttttccattt
      781 tcgtcgaata accatatacc tgcaccagtc cctactccca acaccccgct gggcaatcta
      841 ttcgtccctg ctctccaggc tccatacata tcattgtata cttttatatt ttcaatagat
      901 gtccttaacc ttaagttgtc ttcataatat tttttgatat catcactgtc tacagtagcc
      961 atgcaggcag tcaaggcata aacatcaggc atttcaattc catttctttg tagcaggtct
     1021 ttcagataat ccacttgctg tcccaaatat ctaactgcaa actcagaact atccttgatg
     1081 tcttgaataa atgcactgta tccaaaacca ctgagtttat ctacaattaa tccattttcg
     1141 tctcctaaaa gaatttcggt ttttgaacca ccttggtcaa cgcccacaaa atatctcata
     1201 tgtatctccc actcgtatct tcattttctc aattgtactt tcccacccac catctgtcaa
     1261 taaataacag agtaaaatta actatagaaa cagctatatt agctagatgc gttgtattca
     1321 aaaacatggt gatgtataat atgaacaaaa ttaaaggagt agtcatggga agatttaaac
     1381 atttttctgt actaattgaa tgctcggcgg gcaacacacc aaaagttgat acaataaagc
     1441 attttatacg catgctctcc cgcatggggt acaatgagct ttatctaggc tgcacagatg
     1501 catataaaat ggagggtgaa ccctacttca actataaacg agggggctat actacagaag
     1561 actttcagga gatggatgca tgtgcaaagg aaaacggtat cgaactcata gccagcatac
     1621 aaactcttgg ccatttgcat tttctgcagc gtcatgaatc ttatagggat ttgatggata
     1681 atgaccacgt cctgctggtg ggagatgaac gtgtatacaa actaattgac aaaatgtttg
     1741 ctaccatttc aaaaggacta tccactaagc gcattcacat aggatatgac gagtgttggg
     1801 gcttgggtct tggcaactat ctcaaaaaga atccgcctgc agacaagaaa gagcttctac
     1861 ttcgtcatct ctccaaggtg actaacattg cctccaaata cggatacact tgcgaagttt
     1921 ggcatgatat gctgacagaa accttgaata ccaaagttac cgcagaacaa gtcagaaatg
     1981 cacttccaga aaacacgaca atgttctatt ggaactactt tgaaggcgat gttcatgtgc
     2041 ttagatccgg actagataat atgtttaaat atactgataa agttggatac gcaggcagcg
     2101 tcttcaaatg cggctcaatg gcaccaatga atactttcag cctgtcaaga ctgcttaccc
     2161 agatgcgcgt ggcagaagaa aaaggacttg accattttat gattacactt tggtctgaca
     2221 atggtgcatg ggtcaacaat tatacagtac tgccggcact ttatgcatgt gcagagttca
     2281 acattggacg ttgggacggc acaggcgaat tggacaaagc aaggtttaaa gatattacag
     2341 gggcagacta tgatgacatg ctctcacttg attaccttga tgacccgtat aaaaagaaac
     2401 tgacaaacac tcacgccaat ctcacctttc tcatattctt tgccgacctg ttaaatggtg
     2461 gatgggattt gttgtacgac aaggatatgc caaagactta tgaagacttg gctaaccatt
     2521 atcaaagatt agcctgcgga gattactccc atgttttcaa atgtgcagaa aaactagccc
     2581 gggttctaag cattaagtgc cttttaggac taactattag aaatgcatac aaagcaaatg
     2641 acaccaaaac actgaaggaa tcagtggaaa ggttgaaaga actcgaaaac gctataattg
     2701 agttttcaaa agcatatgct gatatgtggc ttcacgattc tatgccgtat ggtcttgaag
     2761 tatgccagac gttaatagga ggccagatgc ttagaacttc ttatgtcaaa caagttatag
     2821 aagcatatct taaaagcgga cgacccatac cagaactggc atgtgaaaca atagtgccag
     2881 atttgccacc tggcaccaca caagactcct gttggattgc agactggaag ttattgataa
     2941 gcaactgtgg gttttagtca tgcagcagct tcttcaggat aaaagaaatt ctccaata
//
LOCUS       DAONVY010000288         2993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22553_length_2993_cov_2.811777, whole genome shotgun sequence.
ACCESSION   DAONVY010000288 DAONVY010000000
VERSION     DAONVY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22553_length_2993_cov_2.811777"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLI11_07775"
     CDS             complement(<1..599)
                     /locus_tag="PLI11_07775"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPZ52803.1"
                     /translation="MLSIYDTKGNLLKEIEAENGRLLFDILTEAGYRINATCGGMGTC
                     NKCKVNVDGEDVLSCMFKVNSSHKVTLPEYATEKAEAYVLTLSDDMTLGDNPFSEKVG
                     AAIDIGTTTIAAYFYDLETKELIHVDSCLNPQAKHGADVISRIDYTINNNNGTKVMQA
                     LIISCLKKLLSRFDNISICVIAGNTTMLHFLVGEETKTIA"
     gene            686..1888
                     /locus_tag="PLI11_07780"
     CDS             686..1888
                     /locus_tag="PLI11_07780"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HPZ52804.1"
                     /translation="MTICAIICEYNPFHNGHAHHIEETRRITNCDYIISIMSGNYVQR
                     GEPAIMDKWERTRAALKCGSDLVIELPFIYACSSAEYFANAAVDIINKTNVVDYLSFG
                     SDSGDLDYLKNIAAKRINKKLDLTGEIAKAYKEGISYPKAMQLIYDEPELKSNDILGI
                     RYLMALEKSKSSVEPVTIKRKGSEYKDKAITSETPSATAIRKHITDSKDYNLLSKVMP
                     KESMHILMEEIEKGRAPVTLKSFEKQILSTLRTLKPKGIADKPHITPGLEYRLYQNAM
                     ECSNIDEVIDATVSKVFTRTRIQRLLIHALHGVTKEMHANFMDVPYVRVLGFKKRAEP
                     LLKKINRSCNVPVIYRAKKDYENLTDRGKALFELEALSTDIYVLGYPNNMHSNGGQEF
                     SRKIIIVE"
     gene            1960..2373
                     /locus_tag="PLI11_07785"
                     /pseudo
     CDS             1960..2373
                     /locus_tag="PLI11_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(2409..>2993)
                     /locus_tag="PLI11_07790"
     CDS             complement(2409..>2993)
                     /locus_tag="PLI11_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPZ52805.1"
                     /translation="RMNYSIEQYKHFMRETGLFEMLQNHIVSNLVDYAFGVETGLDSH
                     SRKNRGGDSMEDLVEKYIIAAGFVKNINYFKEMKLTEIKNRFGLDLSVFGDEERAEKK
                     FDFVVRADKMIYGIETNFYTGHGSKLNETARSYKMLYQEAQRIDGFKLVWITDGIGWT
                     SAKDNLRETFNVMETVYNINDLEKGLLTSLLTGN"
BASE COUNT          978 a          646 c          553 g          816 t
ORIGIN      
        1 gcaatggttt ttgtctcttc tcctaccaga aaatgcagca tggtggtgtt tcctgcaata
       61 acacaaattg aaatgttgtc aaacctggaa agcagtttct taagacagga tataatcaaa
      121 gcctgcatta cctttgttcc gttattgtta ttgatggtgt aatctattct cgaaataacg
      181 tcagcaccat gttttgcctg cggattcagg caggaatcta catggattaa ttcctttgtt
      241 tcaaggtcgt aaaaataggc agcgatggtg gtagtgccaa tatctatggc agcaccaacc
      301 ttttcactga aaggattatc acccaatgtc atgtcatcgg atagtgtaag tacatacgcc
      361 tcagcctttt cagttgcgta ttcaggtagg gttaccttat gactggaatt taccttgaac
      421 atgcaggaca aaacatcttc accatccacg ttgaccttgc acttgttgca ggttcccatg
      481 ccgccgcaag ttgcatttat cctgtatcct gcctctgtta atatgtcaaa caagagtctt
      541 ccgttttccg cttcgatttc cttcaacaaa ttccctttcg tgtcataaat ggaaagcata
      601 tataatcacc cttttcttgt ttttacattt cggatagtat acactaagaa tggaataaaa
      661 taaagggaga tatcaggagt tttgtatgac aatctgtgca attatatgtg aatacaatcc
      721 atttcacaac ggtcatgcgc accatattga agaaaccaga agaataacaa actgcgatta
      781 tataatctcc attatgagcg gaaactacgt tcaaagagga gagcctgcca ttatggacaa
      841 gtgggaaaga acaagagcag ctctaaaatg cggttccgat cttgtaattg aacttccctt
      901 tatctacgcc tgctcaagtg cggaatattt tgcaaatgcg gcggttgata tcattaacaa
      961 aaccaatgtg gttgattatc tgtcttttgg cagtgattca ggcgatcttg attatctaaa
     1021 aaacattgca gcaaaaagaa tcaacaaaaa attagacctt acaggtgaaa ttgccaaggc
     1081 atacaaggaa ggcatctcat accccaaggc aatgcagctg atatatgacg agcctgagtt
     1141 gaaatcaaac gacatcttgg gtattaggta tcttatggca ctagaaaagt caaaaagttc
     1201 agtggagcct gtaaccatta aaagaaaagg atctgaatac aaggacaagg caataaccag
     1261 cgaaactccc agtgctactg ccataaggaa acatatcaca gattctaaag attataacct
     1321 tttgtcaaaa gtaatgccca aggaatccat gcacattctt atggaggaaa ttgaaaaagg
     1381 aagggcccct gttaccctca agtcctttga gaaacagata ctatcgactc taagaacact
     1441 aaaaccgaag ggaatcgctg acaaacccca tataaccccc gggcttgagt acagactcta
     1501 ccaaaacgcc atggaatgct caaacattga tgaggtaata gacgccacag taagtaaagt
     1561 ctttacaaga accagaatcc aaagactttt gattcatgcg ctccatgggg taacaaaaga
     1621 aatgcatgca aatttcatgg acgtccctta tgtgcgagta ctgggtttca agaaaagagc
     1681 tgaacctctc ttgaaaaaaa ttaacagaag ctgcaatgtc ccggtaatat acagggcaaa
     1741 aaaggattat gaaaacctga cagacagagg aaaagctctt tttgagcttg aagcattatc
     1801 taccgacata tatgtacttg gatatccaaa taatatgcac tctaatggag gccaggaatt
     1861 ttcgcgaaaa attattatag ttgagtgatg cacaatgagt atctattcat cataggataa
     1921 actagagcct atgaaaggag tagtttaatg cctcatcacg aaaaacagga tatgggacct
     1981 gaaccttaca tcctaaacat caagcagaaa gcaaaacaga acacaaacta ccgcacaacc
     2041 atttggacag gagaatattt gcaattaaca ctcatgagca ttgtcacagg tggagatata
     2101 gggcttgaga tgcatccgga ccttgatcag ttcatacgtg tggaagaagg ccagggcctc
     2161 gtccaaatgg gagaaaaaga ggacaatcta agtatccaaa aaagaattta cagcggctat
     2221 gccttcataa tccctgcagg cacatggcac aatctgataa acaccggcat gaaacccttg
     2281 aaactctatt cgatatacgc acctccccaa caccctcctg gaacaataca ccgaacaaaa
     2341 gaaatagcaa tgcaggaaga acaccatgat tagaaaaagg ctattggcat ataaagccat
     2401 agcctttttc aattgccagt caagagtgat gtcaacaatc ctttttctag atcattaatg
     2461 ttgtacaccg tttccatgac attaaaagtt tctctcaaat tatcttttgc agaagtccat
     2521 ccaattccat ctgtaatcca gaccagctta aaaccgtcta tcctttgagc ttcctgatac
     2581 agcattttgt agcttctggc agtctcatta agcttggaac catgaccagt ataaaaattt
     2641 gtttcaatac cataaatcat tttgtctgct cttacaacaa aatcaaactt tttttcagcc
     2701 ctctcttcat ctccaaacac ggacaaatca agaccaaacc tgtttttgat ttctgtcagc
     2761 ttcatttcct tgaaatagtt gatgttctta acaaaacctg ctgcaattat gtacttctcc
     2821 accaaatcct ccatggaatc tccgcccctg ttctttctgc tgtgtgaatc aaggcccgtt
     2881 tccacaccga aagcataatc caccaaattg ctgactatat gattctggag catttcaaat
     2941 agtcccgttt cccgcatgaa atgtttgtac tgctctatgc tgtaattcat cct
//
LOCUS       DAONVY010000289         2983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22654_length_2983_cov_4.594604, whole genome shotgun sequence.
ACCESSION   DAONVY010000289 DAONVY010000000
VERSION     DAONVY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22654_length_2983_cov_4.594604"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..777
                     /locus_tag="PLI11_07795"
     CDS             145..777
                     /locus_tag="PLI11_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52806.1"
                     /translation="MRKLIVFMLVMVVVLSMTLIPSSAEDDLVIDLGAIGGVEKYGPH
                     GYPDQKLLMFGYGTVVKLGNFDLSKYDAMEITYATDLGFQAMKEGMKHVAILGIKSAE
                     SPIFGYANLDFNEENSLGWAPLTDADPEANPDGVNWGKGERKCVIDLSEQNYSGDVYL
                     MCYNSIGNETLVVGIKLIAKQENPSTGDIGIVGMAIVSTFATTLAKKKRR"
     gene            complement(947..1570)
                     /locus_tag="PLI11_07800"
     CDS             complement(947..1570)
                     /locus_tag="PLI11_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52807.1"
                     /translation="MKTLMRKVALILVLVCIFAMVLPVSAAEERIDLYKSGGGDGPKN
                     VLAVGDIAVKFTVPEGKMLKAFVFIDSPTWTKKDSTGEIVLFKWDTDYATTVAGDRLE
                     SHSELHADNSDFRLDVNGYYGPGTYLVLMPKDAATNDLGVWTDGKNPDVGAVTYINGK
                     EVSYMAKSCIYVADDNPPTSDSTTVFAYVLIIAAAGAIVFKKRRAEA"
     gene            1741..2367
                     /locus_tag="PLI11_07805"
     CDS             1741..2367
                     /locus_tag="PLI11_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52808.1"
                     /translation="MHILSIKSLNFKNKIFYKDVNIEKGQIVFLRGASGSGKSTLLRL
                     INATLPLEEGTILYNNKDIREIDTITLRREIILAGQSVYLFPGTIKDNFKRYHEYRES
                     QVPSDEVIRSLLDTSCIDFDIDTVCNNFSGGERQRVFISIALSFKPRVLLLDEPTSAL
                     DNATADRLMSNLVTHCKQNYMTMIIVSHDQTITEKYAEVTIDIGEGVK"
     gene            2364..>2983
                     /locus_tag="PLI11_07810"
     CDS             2364..>2983
                     /locus_tag="PLI11_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52809.1"
                     /translation="MSGAASISLVRFIIIYLLLLIVIAIMKKSKINQTKLLFVASIRM
                     TLQLVIAGFILTYIFKHPHPVFTVLYLASMITFAIHRVLSRNKELNTNFKIVIGFSLA
                     FSGLGIVAYFVMAVVNQSIFNPQYTIPLSGMIVGNAMTGVNLGVKTFLESISAEKPRI
                     EALINFGAKPKDILLPFVNQSLETALLPTLNNMVGMGIVSLPGMMTG"
BASE COUNT          936 a          576 c          568 g          903 t
ORIGIN      
        1 atcttcggaa gaagtaaatg caactgtatc tttgcattta aaaattcaaa tgacctttaa
       61 tgaatatatt ttaaataaac cagtttacat ttggccaatg cttgtatata atatgagtaa
      121 aataatacta ggaggtaatg cataatgagg aaacttattg tttttatgct tgttatggtc
      181 gtagtcctgt caatgacgct aataccctcc tccgctgagg atgatttggt catcgatttg
      241 ggtgctatcg gcggtgtcga aaaatatggt ccccatggct atcctgacca aaaactgcta
      301 atgtttggtt atggaactgt tgtaaaacta ggtaatttcg atttgtcaaa atatgatgct
      361 atggaaatta catatgcaac agacctcgga ttccaagcaa tgaaagaggg aatgaaacat
      421 gtggctattc ttggtatcaa atcagcagaa tctccaattt tcggatatgc aaacctggat
      481 ttcaatgaag agaactccct tggctgggct ccattgacag atgcggatcc agaagctaat
      541 cctgacggag tcaactgggg caaaggcgaa agaaagtgtg taattgacct ttccgagcag
      601 aactacagtg gagatgttta cttgatgtgc tacaactcta taggtaacga aacccttgtt
      661 gtaggaatca agctcattgc aaaacaagaa aacccatcta ctggcgacat cggtattgta
      721 ggtatggcta tcgtttctac ttttgccaca actttggcaa agaagaaaag acgttagtgc
      781 aatgcgttag aacctcaaac agcaggaaat atgggtatca tatccatatt tcctgctgtt
      841 ttttatgtga attaagttct tgtgcgtctt acgaaataga aaaaagggat ttgcattcag
      901 taaatctgat gcgcatcccc ttttaatgat tctgattaat ggaatatcat gcttcagctc
      961 ttctcttttt gaatacaatg gcaccagctg cagctataat cagaacgtat gcaaaaacag
     1021 ttgtagaatc agaagttggt gggttgtcat ccgccacata tatgcaggac tttgccatgt
     1081 aacttacctc tttaccgtta atataggtta cagcacctac atccggattt ttgccatcag
     1141 tccatacacc aagatcgttt gttgcagcat cctttggcat cagaaccaaa tatgtaccag
     1201 gtccataata tccattgaca tcaagtctga agtctgaatt gtctgcatgc agctcggaat
     1261 gtgattcaag tctatcgcct gcaacagttg ttgcataatc cgtatcccat ttgaagagaa
     1321 cgatttcacc tgtgctgtct ttttttgtcc aagtaggact atcaataaaa acgaacgctt
     1381 taagcatttt accttccggc acagtaaact tgactgcaat atcgccgaca gcaagaacat
     1441 ttttgggacc gtctcctcca ccggatttat acaaatcaat acgctcctct gcagcactta
     1501 ccggtaaaac catggcaaat atgcagacta atacaagtat caaggcaact tttctcatta
     1561 aggttttcat aggtacaccc ccgtattttt gctttagcta aattatattt acaatccaga
     1621 tatctgtcaa tagtatttta atattttatt tacactctct aattacagtt attttattgt
     1681 tcttttactt tttagcccac cccggtataa tattatctat acggtttcaa ggagatgtat
     1741 atgcatattc taagtataaa atcattgaat ttcaaaaata aaattttcta caaagatgta
     1801 aacattgaaa aaggacaaat agttttctta agaggtgcca gcggtagtgg aaaaagtact
     1861 cttctaagac ttatcaatgc aacccttcca ttggaagaag gaacaattct ttacaacaac
     1921 aaggatattc gcgagattga cacaattaca ttgagaaggg aaataattct cgcaggtcag
     1981 tcagtatatc tgtttccagg aacaataaaa gataatttca agaggtatca cgagtacaga
     2041 gaatcgcaag ttccttcaga tgaggtaata aggtccttgc ttgacacaag ctgtatcgat
     2101 tttgatatcg acacagtatg caacaacttt tcaggaggag aaagacaaag agttttcata
     2161 agcatcgcct taagctttaa acctagggta ttgctactgg acgagcctac atccgctttg
     2221 gataacgcaa ctgcagaccg gcttatgagc aatcttgtca cacactgcaa gcagaattac
     2281 atgactatga ttattgtaag ccatgatcag accattactg aaaaatatgc tgaggttaca
     2341 atagatattg gggagggtgt aaaatgagcg gagctgcttc aattagtctt gtacgattca
     2401 taataatcta ccttttattg ctgatagtta tagcaataat gaagaaaagc aaaatcaacc
     2461 agacaaaact gctttttgtt gcaagtataa gaatgacatt gcagctggtt attgcaggtt
     2521 ttattctaac atacattttt aaacacccac accctgtatt cacagttctt tatcttgcat
     2581 cgatgataac ctttgctatc cacagagttc tttcgaggaa caaggaattg aatacgaatt
     2641 ttaaaatcgt gattggattt tccttggcct tttcagggct tgggattgtc gcctattttg
     2701 taatggctgt tgtaaatcag agcattttca atccccaata taccattccc ctgtccggca
     2761 tgattgttgg aaacgccatg acaggagtaa atcttggtgt aaaaacattc cttgaatcaa
     2821 tatcagcaga gaaacccagg attgaagctt taataaattt cggagcaaaa ccaaaggaca
     2881 tactgctgcc ttttgtaaat cagtcgcttg agaccgccct tcttcctaca ctaaacaaca
     2941 tggtaggaat gggcattgta tccctccctg gaatgatgac agg
//
LOCUS       DAONVY010000290         2969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22781_length_2969_cov_3.532601, whole genome shotgun sequence.
ACCESSION   DAONVY010000290 DAONVY010000000
VERSION     DAONVY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22781_length_2969_cov_3.532601"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..326
                     /gene="fliQ"
                     /locus_tag="PLI11_07815"
     CDS             60..326
                     /gene="fliQ"
                     /locus_tag="PLI11_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPZ52810.1"
                     /translation="MDQSDAVAILKEALSVVLTVASPILISAIVIGLCVSIIQAATQI
                     NEQSLGFVLKILAIFIALIVFGPWMITRLIDFASMIFDYMAHMV"
     gene            340..1116
                     /gene="fliR"
                     /locus_tag="PLI11_07820"
     CDS             340..1116
                     /gene="fliR"
                     /locus_tag="PLI11_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPZ52811.1"
                     /translation="METEVLFNFTMHFMLILIRIAAIFTFSPVFGRRNVPAMFKIGFS
                     VMLSLILYNLFPAPSNQVFNLYAYTISCLKEMFVGLLIGYVTVMVFSVANMAGQLIDV
                     QIGFGMAQLFSHEMGTQVSINANLLNIVMLICFFITNGHHALIRILANTFYTLPPGNF
                     TINFDMTGVIVSVFVSCIVMGVQIAMPIVAATFITEMIMGILMRTVPQMNMFIIGIPL
                     KVIIGLLLLVLISPMFVQMTQPLFNTMYESINELLGGVAA"
     gene            1113..2195
                     /gene="flhB"
                     /locus_tag="PLI11_07825"
     CDS             1113..2195
                     /gene="flhB"
                     /locus_tag="PLI11_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPZ52812.1"
                     /translation="MILFMAMDDKTEKPTPKKRRDTREKGEVLKSQELSSAVVLLSLV
                     TIIKTFSSYIGLKVSSVFLELRMDTNLESANFDKDFVHNYLIKGLVFILLCIFPILVA
                     AVLTGLLVNYMQIGFLLTTKALRPRFDRLNPINGLKRVFSMRALVELLKSIIKITILG
                     LVLYSTISKKVDEIPTLVTYDLGTSLDFIVDLALEVGMRCGLFLLVIGVLDYFYQWWM
                     FERNLMMTKQEVKEEFKQLEGNPQTKSRIREKQREIGMRRMMHAVPEADVVVVNPTHY
                     AVALKYDPEKHQSPIVLAKGKGYVALRIREVAKENNVEIVENKEVARALYDTTEIGQQ
                     IPESLYRAVAEILAYVYRMKNYRGGY"
     gene            2195..>2969
                     /locus_tag="PLI11_07830"
     CDS             2195..>2969
                     /locus_tag="PLI11_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HPZ52813.1"
                     /translation="MLKIGDIIVSLAIIAIIVLIIIPLGTIALDFLLLLNLVLSIVVL
                     LTSLYITEPLQFSIFPSLLLIITLFRLGLNISATRLILSNSGQAGEVIYTFGNFVISG
                     NVVVGFIVFLILVAIQFIVITKGSERVAEVAARFTLDAMPGKQMAIDADLNSGLIDEE
                     TAKKRRKDVQREADFFGAMDGASKFVKGDAIVGILITIINFIGGIIIGMLQGGMSFGQ
                     VVEVYALATVGGGLVIQLPALLVSTATGITVTRNASEDSM"
BASE COUNT          848 a          527 c          695 g          899 t
ORIGIN      
        1 ggtggatggc tggagcctta cagtaaaaac attggtagag agttttggag gttgaagata
       61 tggaccagtc ggatgctgtt gcaattttaa aagaagcact ttctgttgta ttgaccgtgg
      121 catctccaat acttatatct gccattgtca tcggactttg cgtttcaatt attcaggctg
      181 caacccagat aaacgagcag tctttaggtt ttgtacttaa gatacttgcc atattcatag
      241 ccctgattgt tttcgggccc tggatgatta caagactgat tgattttgca tccatgattt
      301 ttgactacat ggcacatatg gtttaggtgg atttggtaaa tggaaacaga agtacttttt
      361 aattttacaa tgcatttcat gctcatactg ataagaatag ctgctatttt taccttttcg
      421 cccgtgtttg gcagaaggaa tgtacctgcg atgttcaaaa ttggttttag tgtgatgctt
      481 agtttgatac tctacaattt gtttccagca ccaagcaatc aagtgttcaa cctttacgca
      541 tatactataa gttgtttgaa agagatgttt gtcggactgt taattggata cgttactgta
      601 atggtgtttt ccgtggccaa tatggcaggg cagcttattg acgtgcagat tggatttgga
      661 atggcacagc ttttcagcca tgaaatgggt acacaggtat caataaatgc aaacctattg
      721 aatatagtca tgcttatttg cttttttatt acgaacggac accatgcact tattagaata
      781 cttgccaata ctttttatac attgcctcca ggcaacttta ctattaattt cgacatgact
      841 ggcgtaattg tcagtgtgtt tgtatcttgc attgtaatgg gagttcagat tgcaatgccg
      901 attgtggcgg ccaccttcat taccgagatg ataatgggta ttcttatgag aacggttccc
      961 cagatgaaca tgttcataat cggtatacct cttaaggtaa taatcggact tttgttgctt
     1021 gtattgatat cccccatgtt tgtgcaaatg acccagccgc tttttaacac catgtatgaa
     1081 tctatcaatg agctcctcgg gggtgtggcg gcgtgattct gttcatggca atggacgaca
     1141 agactgaaaa gccaaccccg aagaaacgaa gggatacgag ggaaaaaggc gaggttttaa
     1201 aaagccagga actttccagt gcagtagtat tactaagctt ggttacaata attaaaacct
     1261 tttcatcata tatagggctc aaggtttcaa gcgtatttct tgaattgagg atggacacaa
     1321 accttgagag tgcaaacttt gacaaggact ttgtccacaa ttaccttatt aaaggtttgg
     1381 tattcatatt gttatgtata tttcccatcc tcgtggctgc agtccttaca ggattgcttg
     1441 tcaactacat gcagattgga tttctgctta caaccaaggc attaaggcca agatttgaca
     1501 ggcttaatcc cataaacggg ctaaaaagag ttttttcaat gagagccttg gtggagcttt
     1561 tgaaatcgat tattaaaata acgatactag gacttgtgct ttactccacc ataagtaaga
     1621 aggtagatga aatacctact cttgttacat acgatttggg aacatcgctt gattttattg
     1681 tggacctggc cctggaagtt ggaatgaggt gcggactttt tctattggtt attggagttc
     1741 tggactattt ctaccagtgg tggatgtttg aaagaaacct gatgatgaca aagcaggaag
     1801 taaaggaaga gttcaagcag cttgaaggaa atccgcagac aaagagcagg ataagggaaa
     1861 aacaaagaga gattggcatg cgtagaatga tgcatgcagt accggaagcg gatgtggtgg
     1921 ttgtaaaccc aactcactat gcagtagcac taaagtacga tccggaaaag caccaatccc
     1981 ccatagtgct ggcaaaaggt aaaggttatg tggcacttag gataagggaa gttgccaaag
     2041 agaataatgt tgagattgtg gaaaacaagg aagttgcaag agctctatat gacactacgg
     2101 aaatagggca gcagattcct gaaagcctgt acagagcggt ggctgaaata cttgcttatg
     2161 tttacaggat gaaaaattac agaggtggtt attaatgctt aagattgggg atataattgt
     2221 atctcttgcc ataattgcaa ttattgtgct tattatcata ccgcttggca caattgcttt
     2281 ggactttcta ttactgctta atcttgtttt gtcaatagtg gtccttctga catcattgta
     2341 cataaccgag cctctgcagt tttcaatttt cccctcgcta ttattaatca taaccttgtt
     2401 caggcttggt cttaacatat cagccacaag actgattctt tcaaacagcg ggcaggcagg
     2461 ggaagttata tatacctttg gcaactttgt aatatcgggt aatgtggttg taggtttcat
     2521 tgtattcctt atacttgttg ccatccagtt catagttatt accaagggtt cggaaagagt
     2581 tgctgaagtt gcggcaaggt ttaccctgga cgcaatgccg ggcaaacaga tggcaataga
     2641 tgcggattta aactccgggc tgattgatga ggaaactgcc aagaaaagaa gaaaagacgt
     2701 gcagagagag gcggatttct tcggagcgat ggacggtgcc agcaaattcg taaaaggcga
     2761 tgccattgta ggcatactga taacaattat caacttcatt ggtggcataa ttattggaat
     2821 gctgcagggc ggcatgagtt ttggtcaggt ggtcgaagta tatgcgcttg ctacagtggg
     2881 cggtggcctt gtcattcagc tgcctgcact attggtatca acagcaacag gtattactgt
     2941 aacaagaaac gcatcggaag acagcatgg
//
LOCUS       DAONVY010000291         2956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22905_length_2956_cov_3.511892, whole genome shotgun sequence.
ACCESSION   DAONVY010000291 DAONVY010000000
VERSION     DAONVY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22905_length_2956_cov_3.511892"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLI11_07835"
     CDS             <1..487
                     /locus_tag="PLI11_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52814.1"
                     /translation="DEFLRSDVNKLVHVSKLGLTEDKLTAIKLEVLNILEEKKYMALI
                     GIKDFSAFPDLGVEWTPFLLISIVQNYMQYINIVEIPTRNFNLPNCIFVKSDNPYADD
                     YKEIVRWIIKLQHEQKPFKSLVEVRNWLKKEGVILNGIPIYLTARKLLYMDERGKVVI
                     R"
     gene            820..1125
                     /locus_tag="PLI11_07840"
     CDS             820..1125
                     /locus_tag="PLI11_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52815.1"
                     /translation="MVLAEVIMILIADKYFIVQHPHLVINNVNNYVRIILYMPFYWLY
                     QIFDPNVVPRVNYLTSIVPGLLVIPTAGFGYLRGYRGKKFLNKNFDDIKKKLVYKGD"
     gene            1127..2038
                     /locus_tag="PLI11_07845"
     CDS             1127..2038
                     /locus_tag="PLI11_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52816.1"
                     /translation="MEDNRTMETLVKQYAKYISDINPYHNSEMLESFDGSLDDYTEYF
                     DEITEKWFNTFNEELGGIPKEYLYSIKKPENEEETYEVIKLVSLNLIILAPRFFVDYL
                     SEIEYTKPCVKNILQDDVIAKPYNEVYNDKDEYEAFEIYRQAVVLSQAYEDLVDDLLE
                     GIKKCHPANDNILEYIVESLVTMQAFDKVIGFLNDIDEIDMKYLNLLHAITKHKSDDT
                     YRCLRRCFKSINDDSVKHIVVYMLADYGDSRAVPLLRKYAIDLRDRIVNSTEMSEEER
                     KEQNWYFFSVVNTIEQMGGNVEDLKIF"
     gene            2098..2886
                     /locus_tag="PLI11_07850"
     CDS             2098..2886
                     /locus_tag="PLI11_07850"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPZ52817.1"
                     /translation="MIVILKRSNIMLSVLIFVLVLGIVGVNLGLSNKKDGVEASTIPT
                     LNTKGKVVVLDPGHGGEDPGATSMYSGAKEKDLNLAIAFKLKELLEKAGYTVIMTRTE
                     DVLVYEGDNLSNTAKRRQDLLHRKKLMDESNADIVISIHMNGFKQTKYSGAQVFYTKN
                     SPASKKLAISIQDALRENVDPSNKREALMKSEDIIITKDCKVTTVIVECGFLSNEAEE
                     KKLIDEAYQTKLANAIKLGIDKYFTVNVPKPGPSSKPNATSKPN"
BASE COUNT          972 a          455 c          661 g          868 t
ORIGIN      
        1 tgatgagttt ttaagaagtg atgtaaacaa acttgtccat gtttcaaaac ttggtttgac
       61 agaggacaag cttactgcca ttaagctgga agtgttgaac atactcgagg aaaagaagta
      121 tatggctttg ataggcatta aggatttttc cgcgtttcct gatttgggtg ttgagtggac
      181 tccgtttttg ttgatatcga tagtgcaaaa ctacatgcag tacatcaaca ttgtggaaat
      241 acctacgaga aatttcaatt tgccaaactg tatctttgta aagtccgaca atccatatgc
      301 cgatgactac aaggaaatag taagatggat tatcaagctg caacacgaac aaaagccttt
      361 caagtctctt gttgaagtaa ggaactggct caagaaagaa ggcgtcattc taaacggaat
      421 acccatttat ctaactgcca gaaagctttt gtacatggac gagagaggta aagtggtaat
      481 aaggtaaata tattcctgta tattaggaat actatagtat taaaatggaa gattaactgg
      541 tggttttatg aaaggtcata ttcgaggtgt aaaaatgttt atatactgct acctgttagg
      601 tgtttttctg agttgggctt catacccggt gcttatacac ctcacatttg cagtgcctct
      661 tgcgatttca cagtcaatat attcgataat aacaaccttg gtgcttcttt ccttcatata
      721 catcaatctc tatgatttgg gagagaagga cagaacccgg atattggtgc aacttacgaa
      781 ggcaaaggtt ttgtatgtgc ttctgttgga ttcgtgttgg tggtgctggc tgaagttatt
      841 atgatactta tagctgacaa gtactttatt gtacagcatc cccacctcgt tataaacaac
      901 gtaaacaact atgtaagaat catactgtac atgccgtttt actggctgta ccagattttt
      961 gacccgaatg ttgtaccaag ggtcaattat ctaaccagca ttgtgcctgg tcttttggta
     1021 attcctacgg caggttttgg ttacttgaga ggatacaggg gcaagaaatt cttaaataag
     1081 aattttgatg acattaagaa gaagcttgtt tacaaaggag actaagatgg aagataacag
     1141 aacgatggaa acacttgtaa agcagtatgc gaaatatata tctgatatta atccgtatca
     1201 taatagtgaa atgctggaaa gttttgatgg cagtcttgac gattacaccg aatatttcga
     1261 tgaaattacg gaaaaatggt ttaatacttt taatgaagaa ttgggtggta ttcctaagga
     1321 gtacttgtac agtataaaga aacctgaaaa cgaggaagag acatatgaag ttataaagct
     1381 tgtctctcta aatcttatta ttctggcccc caggtttttt gttgactact tgagtgaaat
     1441 tgagtatacc aagccctgtg tgaaaaatat actgcaagat gatgtgattg caaagccata
     1501 taatgaggtt tacaatgata aggatgaata tgaggctttt gaaatatata ggcaggcggt
     1561 ggttttaagc caggcttatg aggatttggt ggatgacctt ttggaaggta tcaagaagtg
     1621 ccatcctgcc aatgacaata ttctggaata tattgttgaa agccttgtta caatgcaggc
     1681 ttttgacaag gtgattggtt ttctaaatga tattgatgaa attgatatga agtatctgaa
     1741 tcttctacat gcaataacaa aacataaaag tgatgataca tacagatgtc tgagaagatg
     1801 cttcaagagt ataaacgatg atagtgtaaa acatatcgtt gtctatatgt tagctgatta
     1861 cggtgattcc agagccgttc ctcttttaag gaagtatgcc atagatctta gagatagaat
     1921 tgtgaatagt accgagatga gcgaagagga aaggaaagaa cagaattggt actttttcag
     1981 tgtggttaac accatagagc aaatgggcgg aaatgttgaa gatttgaaaa ttttctagta
     2041 tatttacact cccttatgaa tatgctttat caaaaggtat gacagggggt gtcatcattg
     2101 atagtaatat tgaaaagaag caacataatg ctgagtgttc tgatatttgt acttgtactc
     2161 ggtattgttg gtgttaattt gggactaagc aacaagaagg atggcgtgga agcatccaca
     2221 atacctacat taaacactaa aggaaaagtg gttgttctgg atccggggca tggtggtgaa
     2281 gatcctggtg ccacaagcat gtacagcggg gccaaggaaa aggatttgaa tcttgcaatt
     2341 gctttcaagc tcaaggaatt gcttgagaaa gcagggtata ccgtaattat gacaaggacg
     2401 gaagatgttc tggtttatga aggggataat ctgtccaata ctgcaaaaag aaggcaggat
     2461 ttgctgcata ggaagaaatt aatggatgag tccaatgccg atattgttat aagtattcat
     2521 atgaatgggt tcaagcagac gaagtattcc ggagcacaag tcttctatac aaagaattct
     2581 cctgccagca aaaagcttgc aattagcata caggatgcct tgagggagaa tgtggatccc
     2641 tccaacaaga gggaagcgct tatgaagagc gaggacatta tcatcacaaa ggattgcaag
     2701 gttacaacag ttatagttga atgcggcttc ctgtctaatg aggcggaaga gaagaagtta
     2761 atagacgagg catatcagac caagcttgca aatgctataa agctgggtat agacaaatat
     2821 tttacagtta atgtaccaaa accaggtccc tcatcaaagc caaatgcaac ttccaaacca
     2881 aattaataaa aatttttact cataaaaaac aaaatatggc gttttgggaa ataatccaaa
     2941 gccttttcac cgtatt
//
LOCUS       DAONVY010000292         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_22935_length_2952_cov_3.263721, whole genome shotgun sequence.
ACCESSION   DAONVY010000292 DAONVY010000000
VERSION     DAONVY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22935_length_2952_cov_3.263721"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLI11_07855"
     CDS             complement(<1..722)
                     /locus_tag="PLI11_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52818.1"
                     /translation="MKKLLYLILLVMFFCTSMVYAEMIEVKFDFENYIAEDDAKLIDD
                     FTYEWEVFTAKAATNYVRVAEEGNNKFLHFNGYAEVRTWDPIDGPYTFSFDARTEETI
                     NVAFFVRGVLPVTRFNPANGGGVDQTLFYLEADWYRENGGKNGSSGLGGSGLYLMPLG
                     SSKIRINIKTYEPDGINVGTAYYDLDWPEATDPTKFFNVKFVDNGSDKVEIFINNKLL
                     ATVTMSNPGVYEEDEYDSNTYY"
     gene            complement(751..1743)
                     /locus_tag="PLI11_07860"
     CDS             complement(751..1743)
                     /locus_tag="PLI11_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52819.1"
                     /translation="MKKACYLLTVGLVFAVIMSMFFVNAAGKLSEYDFSNWEEVERHV
                     LDFEDKTPDANYSEPPEGWRFFHGGGAHSKVVQGDNNKYLNFLGVAQLYLHPAINEQA
                     YVFSIDLHAPTNQNIGIFARAGMERFPFEANQSDFYEHDRTGVTDGFSSLGGSGFYVR
                     AATNKLKLYIKLYDATQPVKIGNALYEFDAGADFTAGYQTVTYVDNGSEVYIYVNDNL
                     IASIEFSESKKYDIGEEDYYSKVAVKDAAGNKVGEHDNALFAVNSVVAFGNRSANVNI
                     DNIKLTTYNIVEAPDTEPETPENPETNDYFAFHISYLILASALVLAIGKRRVNV"
     gene            complement(1866..>2952)
                     /locus_tag="PLI11_07865"
     CDS             complement(1866..>2952)
                     /locus_tag="PLI11_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52820.1"
                     /translation="GRSYVSGKQPNNGKEDFEFSSDTARYGLSFKQQFEYALEMDPPV
                     IMITGWNEWIAGKPTGDDLNYFANTPVKGYTYVDQFNPEFSRDGEPMKIRDGVGFGDN
                     FYYQMIGYVRKFKGLNKIEKAKNQKTININGDISQWDDIGPEFRDTIGDTKFRNEPSY
                     DLDFRYINNTGRNDFDYAKVSQDKDNIYFLVKTVRDIVHADGPNWMNLFIDLDQSHKT
                     GWEGYDFIINRSGSNGKCTIERFKNNSWEFEKVGEARYTINGQYMMISVPKNVLGIKD
                     KIASFDFKWADNSTTTGDVMQFMDLGDAAPNDRFKFRYIGSEYTTSTNPILSDKSNLV
                     VVIVIVVVLLVALEVVVFVVLRKRKGK"
BASE COUNT          811 a          692 c          499 g          950 t
ORIGIN      
        1 ttgtaatacg tgttgctatc gtactcatcc tcttcgtata ctccaggatt tgacattgtc
       61 acggttgcca gcaacttgtt gttgataaag atttcaacct tgtcgcttcc gttatctacg
      121 aacttgacat tgaagaactt ggttggatca gtggcttctg gccagtcaag gtcgtagtag
      181 gcggttccaa catttatacc atccggctca tacgtcttta tattaatcct aatcttgctg
      241 gaacccagag gcattaagta aagaccgctt ccgccaagac cggaggagcc gttctttcct
      301 ccattctccc tgtaccagtc agcctcaaga taaaacaatg tctgatctac gccaccgccg
      361 ttggcgggat tgaatcttgt aacgggcagc acacccctta caaaaaatgc aacatttatt
      421 gtctcctctg tccttgcatc aaaggaaaaa gtgtaaggtc catctatcgg atcccatgtg
      481 cgtacttcag catagccgtt aaaatgcaga aatttattat tgccttcttc agccactctt
      541 acatagttgg ttgcagcttt agcggtaaat acttcccact cataggtaaa atcatcaata
      601 agctttgcat catcttccgc tatataattt tcaaaatcga atttgacttc gatcatttca
      661 gcataaacca tgcttgtgca gaaaaacata accaatagta tcaagtataa tagttttttc
      721 atcagaattc ccctccaatt tggctgctac ttacacattc acccttcttt tgccaattgc
      781 caataccaat gctgatgcaa gtatcaggta tgatatatgg aaggcaaaat aatcattggt
      841 ttcagggttt tcaggagttt caggttctgt atccggagct tctacaatat tgtaagtagt
      901 caatttaatg ttatcaatat ttacatttgc acttctgttg ccaaatgcca caacgctgtt
      961 gacagcaaac aaggcgttgt catgttctcc aaccttgttt ccagcagcat cctttacagc
     1021 aaccttggaa taatagtctt cctctccaat atcatacttt ttactttcag aaaactctat
     1081 tgaggcaatt agattgtcgt tgacatatat gtaaacctcg ctaccattgt caacgtatgt
     1141 aacagtctgg taacctgctg taaagtccgc acctgcatca aactcataca gcgcgtttcc
     1201 aattttgacc ggctgtgtcg catcatagag tttgatatac aacttcagtt tgttcgtagc
     1261 tgcccttaca tagaatccgc tgccgcccaa agaggagaaa ccgtcagtaa caccggttct
     1321 gtcatgctcg tagaaatcag attgatttgc ttcaaaagga aatctttcca tacctgctct
     1381 tgcaaaaata ccgatgtttt ggttcgtagg agcatgcaag tcaattgaga aaacatacgc
     1441 ctgctcgttt atggcaggat gcagatacaa ctgcgcaaca ccaagaaaat tcaaatactt
     1501 gttattatcc ccttgcacaa ccttgctgtg ggcacctcca ccatggaaga atctccagcc
     1561 ttcgggtggt tcggaataat ttgcatccgg tgttttatcc tcgaaatcaa ggacatgcct
     1621 ttctacttcc tcccaatttg aaaaatcata ctctgacagc ttgcccgcag cgttaacaaa
     1681 aaacatggac ataatcactg caaaaaccaa accaacagtc aataagtaac aagctttctt
     1741 cattttgtat cctccttata tctaattttc aaaatcacaa tataatactt ttccttatgc
     1801 caattatatt tcatccaaac tttccatgta tgtagtaaat atattttttg tagtttctaa
     1861 ttgtattatt tgccttttct cttccttagc accacaaaaa caacaacttc aagagctaca
     1921 agcaaaacaa ccacaataac tattaccaca accagattgc ttttgtcaga gagtatagga
     1981 ttggtgcttg tggtatattc ggaacctata tacctgaact tgaaacggtc attgggcgct
     2041 gcatctccaa ggtccataaa ctgcatgaca tctcctgtag tggtggagtt gtctgcccat
     2101 ttaaagtcaa aggaagcaat cttatccttt atacctaata cgttctttgg gacgcttatc
     2161 atcatgtatt ggccatttat agtgtatctt gcttctccaa ccttttcaaa ctcccagctg
     2221 ttgtttttaa atctttcaat ggtgcattta ccgttagagc ccgacctgtt gattatgaaa
     2281 tcataacctt cccatccagt tttatggctt tggtcaaggt caataaacaa attcatccag
     2341 ttaggaccat cagcatgaac gatgtccctc actgtcttta ccagaaaata tatgttatcc
     2401 ttgtcctggg atactttcgc ataatcaaag tcgtttcttc cggtattgtt aatataccga
     2461 aaatcaagat cgtacgaagg ttcgtttctg aactttgtat cccctattgt atccctgaac
     2521 tcaggaccaa tatcatccca ttgggatatg tctccattaa tatttatggt tttctgattc
     2581 ttcgcctttt ctatcttgtt caatcccttg aacttcctta cataccctat catttgatag
     2641 tagaagttgt ctccaaaacc cacgccatcg cgtatcttca tgggctctcc gtctcttgag
     2701 aactccggat tgaactgatc cacatatgtg tatcctttga caggagtgtt tgcaaaatag
     2761 ttgaggtcgt ctcctgttgg tttgcctgca atccattcgt tccagcctgt tatcatgatt
     2821 acaggaggat ccatttccaa ggcatattca aactgttgtt taaaggacaa accatacctt
     2881 gcagtatcag atgaaaactc aaagtcttct tttccgttgt ttggctgttt tccgcttaca
     2941 tagcttcttc cc
//
LOCUS       DAONVY010000293         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23189_length_2926_cov_2.536747, whole genome shotgun sequence.
ACCESSION   DAONVY010000293 DAONVY010000000
VERSION     DAONVY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23189_length_2926_cov_2.536747"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLI11_07870"
     CDS             <1..733
                     /locus_tag="PLI11_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52821.1"
                     /translation="CFFQGMNVRNLESTAIVAASNVSVTSCTFSNVNKEAYTLEITKF
                     PDKEAKNIYIIDFFLNGEFNGFVINSDDENGCPENVLIQRGVFLNYNTEQLTIKSVKN
                     LVVDNNMLDQSALYCVTMNPVYKGIDNVTIKENYIAASYRFGELADTTAILVDGTKDS
                     KITNVVITNNMIAYSVNGMIVNGNNFADSKVIDNVFQSTLSYGLKIEEAINLSIQRNS
                     FKPDASSIGLQIDKQNDKTIVKDNF"
     gene            747..2015
                     /locus_tag="PLI11_07875"
     CDS             747..2015
                     /locus_tag="PLI11_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPZ52822.1"
                     /translation="MKRTALIITMFLLLNTSFAFAHPIDESNHIYINVKDFGAVGDGS
                     TDDTEAVKNAAKKAYAESKVLYFPTGTYVISDAIKFGFDNSKTLTLQGGNDAVITTPE
                     DYEGDIFVSNMEFNFYVKDLKFIHKGTKGQILNTHYLNAYNCIFEGNSTNRSTLVSFA
                     GSNCKVQDSTFITKNENSYALEYVRRPDKISINDYIIDNTFTGVGNGLRIAASSMDGR
                     PEGLKVNGCTFENSGKLQILVECILHLDISDNTFKNSTGTAIKVVPRDLGVHGLFITG
                     NSIQAQEYCIEAQDNGLGTIHIADNYIHDSKIGVFMNKSFADVHVHNNYFKNISDTPV
                     KLLNAFEIYVAENVIEDSGKSLHITGRKGNFVIENNIYSKKAIVDIENYKYKETLSNQ
                     VNIYVVLSIIFSIGIVVLIAVLVFLLKKKT"
     gene            2033..2875
                     /locus_tag="PLI11_07880"
     CDS             2033..2875
                     /locus_tag="PLI11_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52823.1"
                     /translation="MLKRVVSLAIVFFMFFAFTVYSEEDPNANVFESNIDEWTFAGDG
                     IFGKKTNYYEKSGLFMSDRTVQYVYAFSKMQLGSDKSFSFEVVFDVPSYDATGAEKGW
                     CWQNIFIGVKDPDDPGADEYVSLRFQHYPEGNENIQFTSTVSSFSDTVPLPEEKVGYM
                     NHTVLFYYSIVDESLTVTVDGDELFRIEDASEDIEGHLGFGATWCAMRILKAVYTKLD
                     ETPAPDTPTPEQKNTPTPAKTPSPTPKAEKEVDLAKTLAFSLGAVMVVLLVVVVIVAI
                     KKKR"
BASE COUNT          972 a          563 c          606 g          785 t
ORIGIN      
        1 gtgtttcttc caagggatga acgtaaggaa tttggaaagc actgcaatcg ttgcagcaag
       61 caatgtaagt gtaacttcct gtactttctc aaatgtcaac aaggaagcct acactcttga
      121 aattaccaaa tttcctgaca aggaagcaaa aaatatatac ataattgatt tctttttgaa
      181 cggagaattc aatggctttg taataaactc cgatgacgaa aatggttgtc ctgaaaacgt
      241 actaattcaa agaggcgttt tccttaacta caacacagag caactgacga taaagtctgt
      301 caagaatctt gttgtggaca acaacatgct tgaccaaagc gccctgtact gtgttaccat
      361 gaaccctgta tacaaaggta ttgacaatgt tacaataaag gaaaactaca ttgccgcatc
      421 ctacaggttt ggcgagcttg cagacacaac agcaattctt gtggacggca caaaggattc
      481 caagataacc aatgtagtta taaccaacaa catgattgca tactctgtaa acggtatgat
      541 agtaaacggc aataactttg cagacagcaa agttatagac aatgtatttc agtccacact
      601 aagctatggt ttgaagatag aggaagcaat caatttatcc attcaaagaa actctttcaa
      661 acccgatgct tcaagcattg gtctccagat cgacaaacag aatgacaaaa caattgttaa
      721 ggacaacttt taggaggtgt ggacatatga aaagaacagc tttaataatt actatgtttc
      781 ttcttttgaa cacgtcattt gcctttgccc atccaataga tgagagcaat cacatataca
      841 taaatgtaaa agactttgga gcagtaggag acggcagtac ggacgatacg gaagctgtaa
      901 agaatgctgc gaagaaagca tatgctgaga gcaaggtatt gtatttccct accggtacat
      961 atgtgatatc ggatgctatt aagtttggtt ttgacaattc aaaaacacta acactgcaag
     1021 gcggaaatga tgcagtcatt accacacccg aagattatga aggcgacatt tttgtttcaa
     1081 acatggagtt taatttctat gtaaaggatc ttaaattcat ccacaaagga acaaagggac
     1141 agatattaaa tacccactac ctgaatgcat acaactgcat ttttgaagga aacagtacaa
     1201 acagaagcac tcttgtaagc tttgccggtt ccaactgcaa ggtacaagac agcaccttca
     1261 taactaaaaa tgaaaactca tatgctcttg agtatgtaag aaggcctgac aaaatttcca
     1321 taaacgacta tatcatcgac aacaccttta caggtgttgg aaacggtcta aggatagctg
     1381 cttcctcaat ggacggaaga cctgaaggac tgaaagtaaa tggctgtacc tttgagaatt
     1441 caggaaagct gcagattctt gtggaatgca tccttcattt ggacatatcc gacaatacat
     1501 ttaaaaactc tacaggcaca gccataaagg ttgtaccaag ggatttgggt gtgcacggcc
     1561 tcttcattac aggaaacagc attcaggctc aagaatactg tattgaagcc caagacaatg
     1621 gtcttggcac aatccacata gccgacaatt acatccacga ctcaaagata ggcgtattca
     1681 tgaacaaaag ctttgctgat gtacatgttc acaacaacta cttcaaaaac atttcagata
     1741 cacccgtaaa acttctaaac gcctttgaaa tatatgtggc ggagaatgtg attgaggact
     1801 ctggcaaatc cctgcacata acaggcagga aaggaaactt tgttatagaa aacaatattt
     1861 attccaaaaa ggcgatagta gatattgaaa actataaata caaggaaact ttgtcgaatc
     1921 aagtcaacat atacgtggtc ttgtcaatca tcttttccat agggattgtg gttttgattg
     1981 ctgtgttggt attccttttg aaaaagaaaa catgagaaag gagtgggttt tcatgttaaa
     2041 aagagttgta tcgcttgcta ttgtgttttt tatgtttttt gcttttacag tctattctga
     2101 agaagatcct aatgccaatg tatttgagtc gaatatagat gaatggacct ttgcaggaga
     2161 tggcatcttt ggaaagaaga caaactatta tgaaaaaagc ggacttttca tgtcagacag
     2221 gactgttcag tatgtatatg cgttttccaa aatgcaactt ggcagcgaca aatccttttc
     2281 ctttgaagtt gtgtttgatg tgcccagcta tgatgcaaca ggagcggaaa agggctggtg
     2341 ctggcagaac atttttatcg gtgtcaagga tcctgatgac cctggcgctg atgagtatgt
     2401 gtctttaaga ttccaacact atcctgaagg aaatgaaaac attcagttta cgtccaccgt
     2461 ttccagtttc agcgacaccg ttcctctgcc tgaagaaaag gttggataca tgaaccacac
     2521 ggtacttttc tactacagca ttgtagatga aagtcttacg gtaacagtag acggggatga
     2581 acttttccgg atagaagatg caagcgaaga cattgaaggg cacctgggtt ttggtgccac
     2641 ctggtgtgcc atgaggatct tgaaagcagt gtacacgaag ttggatgaaa ctcctgctcc
     2701 tgatacacca acacctgagc aaaagaacac gccaacacct gcaaaaacac caagtccaac
     2761 tccaaaagca gaaaaagaag ttgatttagc aaaaaccctt gccttttctt tgggtgctgt
     2821 catggtggtg ctgcttgttg ttgtggtgat tgttgcgata aagaaaaaga gataaaaaaa
     2881 gtgtctccga cacatcagct cccctagccc ctcaatcttg agggga
//
LOCUS       DAONVY010000294         2920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23270_length_2920_cov_2.609075, whole genome shotgun sequence.
ACCESSION   DAONVY010000294 DAONVY010000000
VERSION     DAONVY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23270_length_2920_cov_2.609075"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLI11_07885"
     CDS             complement(<1..329)
                     /locus_tag="PLI11_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPZ52824.1"
                     /translation="MRFRPCIDIHEGKVKQIVGSSISDNSVLENFVSTKDAEYYANLF
                     KKDNLKGGHVIKLCKSKKTEEEAIKALKAFPSGMQIGGGINADNCLKYLSLGASHVIV
                     TSFIFHDG"
     gene            429..1160
                     /locus_tag="PLI11_07890"
     CDS             429..1160
                     /locus_tag="PLI11_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52825.1"
                     /translation="MLSIREVSKTYGKRQVPAVDNLSLEIKEGEIFGFIGPNGAGKTT
                     TIKMLTGILPPDKGSIVIAGVDLVKNPVEAKRKIGYVPDNPNIYERLTGMEYLEFIGD
                     VYGVPYAERRERINKYMKMFEMEFSFNDQIRSYSHGMRQKLLITGALMHNPKVWILDE
                     PLTGLDPRSSFLLKEQMKEHKKNGNTVFFSTHILEVAEKLCDRIGIIDKGRLILVGTI
                     EELKEKAQKNSSLEDVFMTITDQEG"
     gene            1163..2644
                     /locus_tag="PLI11_07895"
     CDS             1163..2644
                     /locus_tag="PLI11_07895"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52826.1"
                     /translation="MNRFKKLLFFRLKNMLGIPQLKYMMKNNEKSQLYLAGFLLLFLI
                     VAASLLAPYFLILNFAYEATEILGDTTLFISLLTSSGQILLLFTGILYCFNSMYTSRD
                     SEFLLSLPFPRGYLFLTSYVIAYIMNFFTSLLLLLPGLVVYLYKAGDAVVFVKGLVLS
                     ILYPIIPVSLSMIIVIPIMHILNRFKHKDIVATVAGFVLMVLFIASNMVFSSRMQEGQ
                     EAEFFAKLFSERIDLPKLTKGLVPYSAFMKEFLVNGKIGGATSFVVLAVLSAFVVIVA
                     YMLCRRLYESLQQKIGHAGKKEKAVDIHAIKVGNPRLAFCKKEIKLILRSPVYALNCL
                     LNIVLGPLAVISIFKLSTNSEEAVALDALVESFRDKPYVLLYIFYGFVMFLTSLNFAP
                     STTFSREGEHFWLTKVIPVPYVEQVKGRIYASVIFYLCCCVTMTVLLQFFIRLKLVYF
                     LSVIVLILASVLQITYMSLAVDLIKPKLHWSTEAEAVRGISTR"
     gene            2650..2826
                     /locus_tag="PLI11_07900"
     CDS             2650..2826
                     /locus_tag="PLI11_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52827.1"
                     /translation="MLLSLVLVVVNALPVVLSIKVNADFGIALSLAISIAIAIFLRNV
                     LMRLVNERYELINV"
BASE COUNT          854 a          482 c          627 g          957 t
ORIGIN      
        1 ccatcgtgaa atatgaaaga agtaacgata acatgggatg cacctaaaga aaggtatttt
       61 aggcaattgt ctgcattaat gcctcctcca atttgcattc ctgaaggaaa ggctttcaat
      121 gcctttattg cttcctcttc agtctttttt gacttgcaga gttttataac atggccgcct
      181 ttgaggttgt ctttcttaaa gagatttgcg taatattccg catcttttgt tgaaacaaaa
      241 ttttcaagca cactattatc gcttatagag cttccaacta tctgctttac ttttccttca
      301 tgtatgtcaa tacagggcct gaatctcata attgcctcca tgctaacctt gataaggata
      361 ttaaatttat ggtatacttc tgtattgtaa cataaacaaa atgctgtggc aaagaggaga
      421 tgggtttagt gttatcaata agggaagtta gcaaaactta tggaaaaaga caagtccctg
      481 ctgtagacaa ccttagtctt gagattaagg aaggagaaat ctttggattt attggtccga
      541 atggcgcagg aaagactacg acaataaaga tgctgactgg cattcttcca cctgacaaag
      601 gttccatagt aatagctggc gttgatctgg tcaagaaccc cgttgaagca aaaaggaaaa
      661 tcgggtatgt gcccgacaat ccaaacatat atgagagatt gaccggcatg gaatatctgg
      721 agttcattgg agatgtatac ggtgttccat atgcagagag aagggaaaga atcaacaagt
      781 atatgaagat gtttgaaatg gagttttcct ttaacgacca gataagaagc tattcccatg
      841 gcatgaggca gaagctgctc atcaccggag cacttatgca taacccaaag gtctggattt
      901 tggatgaacc actaaccggt ctagacccta gatcatcctt tttgctgaaa gagcagatga
      961 aagagcataa aaagaatggc aatacagtgt ttttctcaac acatattctg gaggttgcag
     1021 agaaactttg cgacagaata ggaataattg acaagggaag acttatcctt gtaggtacta
     1081 tagaggagct taaggaaaaa gcccaaaaga attcttcatt ggaagatgtc tttatgacta
     1141 ttaccgatca agagggataa ttatgaatag atttaaaaaa cttctgtttt ttaggcttaa
     1201 aaacatgctg ggcatacccc agctgaaata tatgatgaag aacaatgaaa aaagccagtt
     1261 gtatctcgca ggctttctac tattgttttt aatagtggca gcatccttgc ttgcaccata
     1321 ttttcttatt ctaaattttg catatgaggc aacagagatt ttgggagaca caaccctttt
     1381 tatctctctg cttacttcgt ctggacaaat tcttttactg tttacaggaa ttctctattg
     1441 ttttaattcc atgtacacat ccagggatag cgagtttttg ttatcgctgc catttccgag
     1501 aggatatctg tttcttacgt catatgttat tgcatacatc atgaatttct ttacaagtct
     1561 tttgctgctt ttgccagggc ttgttgttta cctgtacaaa gcaggtgatg ctgttgtctt
     1621 tgttaaagga ctggttttat caatacttta tccaataata ccggtatcct tgtcgatgat
     1681 tattgtaata ccaataatgc atattttaaa cagattcaag cacaaggaca ttgttgcaac
     1741 agtggctggt tttgtgctta tggttctttt tatagcttca aatatggttt tctcatcacg
     1801 tatgcaggaa ggccaggaag cagaattctt tgcaaaacta ttcagtgaaa gaattgactt
     1861 gccaaagctg acaaaggggc tggtccctta ctctgcattt atgaaggagt tcctggtaaa
     1921 tggaaagatt ggcggagcaa caagctttgt tgttcttgct gttttgtctg cttttgttgt
     1981 tattgtagct tatatgcttt gtagacggct ttatgaaagt ctgcagcaaa aaatcggaca
     2041 tgcaggaaag aaagaaaaag cggtggatat acatgcaatc aaggtaggaa atccaagact
     2101 ggcattttgc aaaaaggaaa ttaaacttat tttgcgaagt cctgtttatg ccttgaattg
     2161 ccttttgaat attgttttgg gaccgcttgc tgtgatttca atatttaagc tgtccacaaa
     2221 cagcgaagaa gctgttgcgt tggatgcttt ggtagaatcc ttcagggaca aaccatatgt
     2281 tcttctatac attttctatg gttttgtaat gtttttgacc tccttgaatt ttgctccatc
     2341 aactacattt tcaagagaag gcgagcattt ctggcttaca aaggttattc ctgttcccta
     2401 tgtggagcag gttaagggta ggatttatgc cagtgtaata ttctatcttt gctgctgtgt
     2461 tacgatgaca gtacttctac agttctttat caggcttaag cttgtttatt tcctttctgt
     2521 aattgttttg atactggcaa gtgttttaca gataacatat atgagtttgg ctgttgattt
     2581 gataaaacca aagctgcatt ggtcgacaga agcggaagcg gtaagaggaa tttcaacgcg
     2641 gtaattggga tgcttttgtc attggtgctg gtggttgtaa atgcattgcc agtagttttg
     2701 tcaattaagg ttaatgccga ttttgggatt gctcttagtc ttgcaatatc cattgctatt
     2761 gcaattttct taagaaatgt gctaatgaga ttggttaatg aaagatatga attaataaat
     2821 gtttagaaga ggtgtattat ggtagaactt attaacgagg gtgtatttta caaaaacggt
     2881 gaattggttg cagacaagaa tgcaggtttt gaggaggttg
//
LOCUS       DAONVY010000295         2913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23348_length_2913_cov_3.062631, whole genome shotgun sequence.
ACCESSION   DAONVY010000295 DAONVY010000000
VERSION     DAONVY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23348_length_2913_cov_3.062631"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLI11_07905"
     CDS             <1..1082
                     /locus_tag="PLI11_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HPZ52828.1"
                     /translation="AVLYMGYLGLETGNAICDVLSGKVNPSGRLVSTWPSKYEDVPSS
                     YHFPGNAATSIYFEDIYVGYRFYETFEIEPAFEFGYGLSYTSFEYSNFKMTKKGNKIE
                     FKVTVRNTGKRAGRDVVQVYVTKPGSIQEQPKYELVAFAKTGDLKPGKKETLTLVVKE
                     EDLKTYVSDTSRWMVEGGKYVFSIGSSVKKIHKTYETELEEKVIQDVENRCVPVNEFN
                     VLNQKDGLPSFRRSANVALNKKATCNVHEGDLRAQNAVDGSYSTRWSGLAPSNDSMNW
                     LKVDLEAVYDIENIIIFWEACNVFYRVETSVDGTNWTKILEKSSNPSLYDDIKVEGEV
                     RYIRISGEKNGWFSIYELEAFEKAG"
     gene            1285..1458
                     /gene="rpsU"
                     /locus_tag="PLI11_07910"
     CDS             1285..1458
                     /gene="rpsU"
                     /locus_tag="PLI11_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPZ52829.1"
                     /translation="MPEVRVKENESLDSALKRFKRYCARDGVLAEYRKREHYEKPSVR
                     RKKKAEAARKRKY"
     gene            1558..>2913
                     /gene="recJ"
                     /locus_tag="PLI11_07915"
     CDS             1558..>2913
                     /gene="recJ"
                     /locus_tag="PLI11_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPZ52830.1"
                     /translation="MFEKSWRIRSRCMSEEDTGFLARKFNVPEYIIDILSKRGVCSEE
                     DIESFLNPQLNMLTDPMMLKDMEEAVERILHSSDIGEKVTIYGDYDADGITSTSILYH
                     FLTCFLDIDVDFYIPDRLSEGYGMSVEAIDYISSRGTSLIITVDCGIRNHEEIEYAKS
                     KGIDVVVTDHHQCGETIPDCVACVNPTRPDCPFPHKDLAGVGVTFKLMSALGYAIGLD
                     EEVYEYLPIVAIGTIGDAVPLTGENRIYVKNGLEMVERGVWIGLTKLIELSNGKNGVN
                     LTATYIAFNVVPKINAAGRMGEASRAVYLLLTDDEDVAGGYAMELVSENARRQELEAK
                     TLQEALLPENLKSKDEDSVVIAVGKNWHHGVIGIVAARLMERFNKPAIVLAYEEDSDF
                     LVRGSARSVEGFNLYKGLSMAEDLLIKFGGHEMAAGLTMDVANIPKVIEILNEYAEKE
                     AE"
BASE COUNT          918 a          457 c          743 g          795 t
ORIGIN      
        1 atgcagtttt gtacatggga tatcttggtc ttgaaacggg gaatgccatt tgtgacgtat
       61 tgtctggaaa agtaaatccc tcaggcagac tggtctcaac atggccaagt aaatatgaag
      121 acgtgccttc ttcataccat tttccaggca atgcagctac ctccatatac tttgaagata
      181 tttatgtggg ttacaggttc tatgaaacct ttgaaataga gcctgccttt gagtttggct
      241 acggcctgtc ctacaccagt tttgaatatt caaacttcaa aatgacgaag aaaggaaata
      301 aaattgaatt caaggtaact gtcaggaata ctggtaaaag ggcaggcagg gatgtagtcc
      361 aggtatatgt gacaaaacct ggttctattc aggaacagcc aaagtatgaa cttgttgcct
      421 ttgccaaaac aggcgatctt aagcctggta aaaaggaaac ccttacattg gttgtaaagg
      481 aagaagatct aaagacatat gtttcagata cttcaagatg gatggtggag ggtggcaagt
      541 atgtgttctc cataggttct tctgtaaaga aaattcacaa aacatatgaa acagaactgg
      601 aagaaaaggt tatccaggat gtagaaaaca gatgtgttcc tgtaaatgag tttaatgtat
      661 tgaatcaaaa ggatggattg ccttccttta gaagaagtgc caatgttgct ctaaacaaga
      721 aagcaacctg caatgtgcat gaaggagatt tgcgtgcaca gaatgcggtg gatggctctt
      781 actccacaag atggagtggt ttggctcctt caaatgacag catgaactgg ctgaaagtag
      841 atctggaggc ggtttatgat attgagaata tcataatatt ctgggaagcc tgcaatgttt
      901 tttacagggt ggaaacctct gttgatggta ccaattggac aaagattttg gaaaagagct
      961 ccaatccctc tctttatgat gatattaagg tggagggaga ggttagatat attaggatta
     1021 gtggtgagaa gaacggttgg ttcagtatat atgagcttga agcttttgaa aaggcaggtt
     1081 gagcttgatt tttacaataa ggggtaaact taaaagggtt accccttatg atggttcgat
     1141 tcaaaaatta ttagttgaca cagcttgttt atatgatata taatgtgtta tgcgacacat
     1201 tcttttttaa taaggcttag gtgaatcgtc gaattgaaag gaaaattgtg tcttaaaccg
     1261 caaccgtgga ggggagggga tgtcatgccc gaagttagag ttaaggaaaa cgagtcgtta
     1321 gatagtgctt tgaagcgttt caaaagatac tgtgcaagag acggtgttct cgccgaatat
     1381 cgcaagaggg aacattacga gaagcccagt gtaagaagaa aaaagaaagc tgaagctgca
     1441 aggaaacgca aatattaata attgtttctt ttccaaagcg gttatagttc aaaaactaac
     1501 aaagaccttc tgttgcgagg tctttttgtt ttatattgca gtacaaggag aaaataaatg
     1561 tttgaaaaaa gctggagaat ccgcagtcga tgcatgagtg aagaagacac cggtttttta
     1621 gcaagaaaat tcaatgtgcc tgaatacata attgacatat taagcaaacg gggcgtttgc
     1681 agcgaggagg atatcgaaag ctttctaaat cctcagttga acatgttgac ggatccaatg
     1741 atgcttaagg acatggaaga agccgtggag agaatacttc acagcagcga cattggagaa
     1801 aaggtcacaa tatatggcga ttatgatgct gatgggataa catccacaag catactgtat
     1861 cattttctga catgttttct tgatattgat gttgactttt acattccaga caggttgtcg
     1921 gaaggctatg gcatgagtgt ggaagccatc gattacataa gttcaagagg aacaagcttg
     1981 attattactg tcgattgcgg cataaggaat catgaagaaa tagagtatgc caaaagcaag
     2041 ggtattgatg tagttgtaac agatcatcat cagtgtggtg aaacgattcc ggattgcgtt
     2101 gcatgcgtaa atccaacaag gccggattgt ccatttccac acaaggatct ggcaggggtt
     2161 ggagttacgt tcaaacttat gtccgcactt ggctatgcca ttggtcttga tgaggaagtt
     2221 tatgaatacc tgcctattgt tgcgattggt acaattggag atgcggttcc cttaacgggt
     2281 gaaaaccgca tctacgtgaa aaatgggctt gagatggtgg aaagaggagt ctggattggt
     2341 ctaacaaaat taattgaact ttccaacggt aaaaatggtg tcaatctgac tgcaacgtac
     2401 atagcattta atgtggtgcc gaaaataaat gcggcgggca gaatgggtga agcctcaaga
     2461 gctgtatatc ttcttcttac tgatgacgaa gatgttgcag gaggctatgc catggaactt
     2521 gttagcgaga acgcaagaag gcaggagctt gaggcaaaga ctttacagga agccctgttg
     2581 cctgagaatc taaagtcaaa ggatgaggat tcggttgtaa ttgcggtggg gaaaaactgg
     2641 catcacggcg ttatcggtat agtagctgca aggcttatgg agaggttcaa caagccggca
     2701 atagtgcttg cttatgaaga ggattcggat tttcttgtca gaggttcagc aagaagtgtg
     2761 gaagggttca atctctacaa aggactttca atggctgaag atttgctgat taagtttggt
     2821 ggacacgaga tggcggcagg ccttacaatg gatgtggcca acataccaaa agtgattgag
     2881 atattgaatg agtatgcaga aaaggaagct gaa
//
LOCUS       DAONVY010000296         2892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23565_length_2892_cov_4.127952, whole genome shotgun sequence.
ACCESSION   DAONVY010000296 DAONVY010000000
VERSION     DAONVY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23565_length_2892_cov_4.127952"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLI11_07920"
     CDS             <1..1103
                     /locus_tag="PLI11_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPZ52831.1"
                     /translation="FNFSVYPASQPLYFELVNNGYIPELMLSGAVIRSAFCGPCFGAG
                     DVPAHNGLSIRHTTRNFPNREGSKPSEGQISWVALMDARSIAATAANGGVLTPATDVC
                     SEEKEYEYRFNKTVYENRVYNGFGKPMPEHELRFGPNIADWPKMQQLGENILLEVASV
                     ITDPVTTTDELIPSGETSSFRSNPLRLAEFTLSRKDPNYVGRAKQIQALEKARLNSEY
                     DKLDAVFSGIGDAKELAPVTQIGSVVYAVKPGDGSAREQAASCQKVLGGYANIACAYA
                     TKRYRSNLINWGILPFTTLNGEHVEFNVGDRIFIKGIRKAVEDGSEQVNAKVYGIDGV
                     RDINLCLNDLSDNEREILLSGCLINYYNIQED"
     gene            1105..1725
                     /locus_tag="PLI11_07925"
     CDS             1105..1725
                     /locus_tag="PLI11_07925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPZ52832.1"
                     /translation="MNRIVRNVIGFLLVFGIVFAITGCKKEEDYSNVENPIVTMEFEE
                     FGVVEIELYPQVAPNTVKNFIHLIENGFYDGLTIHRIVPGFVIQGGDPNGDGTGGPGY
                     TIKGEFKSNGYNKNNLKHTQGVISMARRPTSNNSAGSQFFIMLADHSSLDGDYAAFGK
                     VISGMDVVFDISTVATNKERPVDPVVIKKVTVDTKGHTYSEPDKIK"
     gene            1744..>2892
                     /gene="uxaC"
                     /locus_tag="PLI11_07930"
     CDS             1744..>2892
                     /gene="uxaC"
                     /locus_tag="PLI11_07930"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPZ52833.1"
                     /translation="MKEFYGDDVFLENEVAVELYNAVKELPIYDYHCHMSAKDIFEDK
                     QFDNIGEMWLGGDHYKWRAMRQCGINEELVTGNASMHDKFIAFASILPKLPGSPIYQW
                     AHMELKTVFGISDPLSADNAEEVWQEANKVIKEKGYSPSKMLELFNVEVVCTTDDPID
                     DLEYHKKLKDYKTKVRPTYRPDRIMYIEKDDFVEYIGKLGADSFDSLLKISEDRLRYF
                     IDAGCKVSDHAISKFPKVQGTYEEAKNAFDKKMKGMQLDQNEMDAYVDYMLLFFIGLY
                     KKHDITMQLHMSPLRNANTNMFKAVGADTGFDTIGTAVTAEKLVDILDRANTQTGLPK
                     IVFYSLNPVADNMIATVIGSFSEDIPGKMQLGSAWWFNDHVKGIERQLT"
BASE COUNT          930 a          494 c          676 g          792 t
ORIGIN      
        1 gattcaattt cagtgtatat cctgcaagcc agcctctgta ctttgagctc gttaacaatg
       61 gatacattcc cgagcttatg cttagtggtg cggttatcag aagcgctttt tgcggtccgt
      121 gttttggtgc tggagacgtt cctgcgcata atggattgag tatcaggcat actacacgaa
      181 acttcccgaa cagagaaggc tcaaaaccat ccgaaggcca gatttcatgg gtagccctga
      241 tggatgccag atctattgca gccactgctg ccaatggagg tgttttgaca cctgcaacgg
      301 atgtttgttc agaagaaaaa gaatatgagt ataggtttaa taaaacagtt tatgaaaaca
      361 gggtgtacaa tggctttggc aagcccatgc cggaacatga acttaggttt ggtccgaata
      421 ttgctgactg gccaaaaatg caacaactgg gcgaaaatat acttcttgag gttgcctcgg
      481 ttataactga cccggttaca accacggatg aacttatccc cagcggtgaa acttcttcat
      541 tccgttcaaa cccattgaga cttgcagagt ttactctatc gagaaaagat ccaaactatg
      601 taggaagagc aaagcagatt caagctttgg aaaaggcaag acttaatagt gaatatgaca
      661 agcttgatgc tgtatttagt ggtataggcg atgcaaagga acttgctcct gttactcaaa
      721 taggcagtgt agtatatgcc gtaaagcctg gagatggttc tgctagagag caagctgcat
      781 cttgccagaa agtgcttggt ggatatgcga atatagcatg tgcttatgca acaaagagat
      841 acagaagcaa ccttatcaac tggggaattc tgccttttac tacattgaac ggagaacatg
      901 ttgagttcaa tgtaggtgac agaatattta ttaaaggtat caggaaggct gtagaggacg
      961 gaagtgagca agtaaatgcc aaagtatacg gcattgatgg agttagagac atcaatcttt
     1021 gtctaaatga tttgtcagat aatgaaagag aaattttgct ttcgggctgt ttgataaatt
     1081 actacaatat tcaggaggat tgacatgaat agaatagtta gaaatgtaat tggttttttg
     1141 ttggtttttg gtattgtatt tgccataaca ggctgcaaaa aagaagagga ttattcaaat
     1201 gttgaaaacc ctattgttac tatggagttt gaagaatttg gagttgtgga aatcgaactt
     1261 tatccacaag tggctccaaa tacagtcaag aactttattc acttaatcga aaacggtttt
     1321 tatgacggtc tgacaattca cagaattgta cctggttttg taatccaggg aggagatcca
     1381 aatggtgatg gtaccggagg tccgggatat acaataaaag gagaattcaa gtcaaacgga
     1441 tataataaaa acaatctaaa acacacccag ggagtaatat ccatggccag aagaccaacc
     1501 tcaaacaatt ctgccggctc ccagtttttt ataatgcttg cagaccattc atctctcgat
     1561 ggagattatg ctgcgtttgg aaaagtaatt agtggcatgg atgttgtttt tgatattagt
     1621 actgtagcga caaataaaga aagaccagtt gaccctgtgg ttataaaaaa ggttacggtg
     1681 gatacaaaag gacacactta cagtgagcct gacaaaataa aataacaatg aagtaggtga
     1741 cttatgaaag aattttacgg agatgatgtt tttttggaaa atgaagtggc agtggaattg
     1801 tataatgctg taaaagaact gcccatatac gattatcatt gccacatgtc agcaaaagac
     1861 atttttgagg acaaacaatt tgacaacata ggcgaaatgt ggttgggcgg cgaccattac
     1921 aagtggcgtg ccatgagaca gtgcggaatc aatgaagaac ttgtaaccgg aaacgcaagc
     1981 atgcatgaca agtttatagc ttttgcctca atattgccaa agcttccagg cagccctata
     2041 tatcaatggg cgcatatgga gctaaagact gtatttggca ttagcgatcc tttaagtgct
     2101 gacaatgctg aagaagtctg gcaggaggca aacaaggtaa taaaagaaaa ggggtatagt
     2161 ccttcaaaaa tgcttgagct ttttaatgtc gaggttgtat gcaccacgga tgacccgatt
     2221 gatgatttgg agtaccataa gaagcttaag gattacaaaa ccaaggtgcg tccaacatac
     2281 agacccgaca gaataatgta tattgagaag gatgactttg ttgagtacat tggaaagctt
     2341 ggagcagatt cctttgattc attgttgaag atttcagaag acagactgcg ttattttatc
     2401 gatgcaggtt gcaaagtgtc cgatcatgca atatccaagt tcccaaaagt ccaagggact
     2461 tacgaagaag caaaaaatgc ttttgataaa aagatgaaag gcatgcaact tgatcaaaat
     2521 gaaatggatg catatgtgga ttacatgctt ttattcttta ttggattgta taaaaagcat
     2581 gacattacca tgcagctcca catgtcgccc cttagaaatg cgaacacaaa catgtttaag
     2641 gcagtaggag ctgacacggg ttttgatacc attggaactg ctgtaacagc agagaagctt
     2701 gtagatattc tggatagggc aaacactcag acgggattac ccaagattgt attctattca
     2761 ttgaatcctg ttgcagataa tatgatagct acagttattg gttcgttttc agaagatatc
     2821 cccggaaaga tgcagcttgg ttctgcctgg tggtttaacg accatgttaa ggggattgaa
     2881 aggcagctta cc
//
LOCUS       DAONVY010000297         2884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23654_length_2884_cov_3.439731, whole genome shotgun sequence.
ACCESSION   DAONVY010000297 DAONVY010000000
VERSION     DAONVY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23654_length_2884_cov_3.439731"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLI11_07935"
     CDS             <1..595
                     /locus_tag="PLI11_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52834.1"
                     /translation="TQKLHTPIKLGCALFYAYPEPTPSPTPDPDAEVKAILYRFAFEG
                     EDIVDTMFEGFIRPTNAELEFTEEGLVITVITKPDPYFYIPFPDGFLELSEYPYLKMF
                     FKHTTGVTRGQFYIAFDGEKISGPDQHYDFQADPSEEWQEVIINLGELKEGAETITAL
                     RADILASPPLDSTITVDYIGFFATLEEAENYVPPNRR"
     gene            complement(644..1972)
                     /locus_tag="PLI11_07940"
     CDS             complement(644..1972)
                     /locus_tag="PLI11_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPZ52835.1"
                     /translation="MKHMKKKILLIQSTPYDSQGKLIKKSRLYFVGLALPLLAALTPD
                     DWQVEICLETIEDVPFDTDCDVVGISSMGHAVIRSIDIAKEFKKRGKIVLMGGYMTSL
                     MPNEAKKYCDSVIIGDAEEVWEDVLRDIENNTLKDYYEKPLTTLKYPTPRFDLIMDKK
                     IGNFLPVQAGRGCPHSCSFCSVACLYKNKYYRREISEVVRDIKQIKELGFNRFLLLDD
                     NIASDPGYMLELCQEIKKLDLKWMSQCSINIARNDKLLNVVAESGCDVLSFGLESISK
                     ESLDAMNKRWANPKEYNVLLKKIRDAGIDVSTEMVVGADGDTLETIKDTAKFIEVNKV
                     AVPRFYILTPIPGTAFFEEMKKENRIYIEDIYSYNGSECVHIPKNMSPEELTKAYWDL
                     YNNVFKIRSILKRTIFQKRFFKKPLTYIFYFYVNLYYRYQIKRKITPNII"
     gene            complement(1969..>2884)
                     /locus_tag="PLI11_07945"
     CDS             complement(1969..>2884)
                     /locus_tag="PLI11_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPZ52836.1"
                     /translation="LEKEKSQTFCYPFPDREKVKKYRKKYYYMFHRNCTLIKTSFGCP
                     YNCSFCFCREITDNKYFARSVEDIINELKTIKEEEVYIVDDNFLFNANRLNHFCDMLE
                     KENIKKRYLVYGRADFIAENEEVVKRLKDNGLRAVIVGLESCDDIMLEGYNKKSNSKI
                     NEKAVAILKKHNIECYGTFILGIDWDREDFKKLKEWIKKLGITFVNLQPFTPLPGTDM
                     YDLYKDKLIVKREEYEKWDMAHLVVRPTKISVRKYYLYTILLYYSTMVNPKKSIYMIK
                     KYGLMDTLKLSIGAFKVNMQYLKKFLKG"
BASE COUNT          783 a          598 c          509 g          994 t
ORIGIN      
        1 tactcaaaaa ttgcacactc caattaaatt ggggtgtgca ctattttacg cttaccctga
       61 accaacacct tcacctacac cggatcctga tgcggaggta aaggcaattt tataccgctt
      121 tgcattcgag ggggaggata ttgttgatac tatgtttgag ggatttataa gacctacaaa
      181 tgcagaattg gaatttacag aagaaggtct ggtgataaca gttataacca agcccgatcc
      241 gtatttctat attccattcc ccgatggttt tttggaactc tcggagtatc catatctcaa
      301 gatgttcttt aagcatacaa caggagtaac aagaggacag ttttacatag catttgatgg
      361 agagaaaata tccggtcctg accagcatta tgattttcaa gcggatccat cggaagaatg
      421 gcaagaggtt atcattaacc ttggtgagct gaaagaggga gcagaaacca tcactgcttt
      481 acgtgcagac attcttgcca gtcctccact tgattccaca ataaccgtag actatattgg
      541 gttctttgca acacttgagg aagcggaaaa ctatgttccg cccaacagaa ggtaatagtg
      601 gatcaataaa gaatcggggc tcgtgccccg attcttttat tatctagata atatttggtg
      661 taatttttct ttttatctga tacctgtagt acagatttac atagaagtaa aagatatagg
      721 tgaggggttt tttaaagaac cttttttgga atatagttct ttttagaatg cttcttatct
      781 tgaaaacatt attatacaaa tcccaatatg ctttggtaag ctcttccggt gacatgtttt
      841 ttgggatgtg cacgcattcg cttccattgt atgagtatat gtcttctatg taaatcctgt
      901 tttccttctt catttcctca aagaaagcag tacctggaat aggggtcagg atgtagaatc
      961 tggggactgc gaccttgttg acttcaataa atttggcggt gtcttttatt gtctcaagag
     1021 tatcaccatc cgctcccacc accatctcag tagaaacatc aatacctgca tccctgattt
     1081 tcttgagcaa aacattatac tccttcggat ttgcccatct tttgttcatt gcatcaagac
     1141 tttcctttga gatgctttca agaccaaaac tcaatacatc gcaaccactt tctgcaacga
     1201 cattcaaaag tttgtcgttc ctcgcaatgt ttattgaaca ctgactcatc cacttcaagt
     1261 caagcttctt aatctcctga caaagttcaa gcatataacc tgggtccgat gcaatattgt
     1321 catccaagag aagaaatctg ttaaaaccaa gctcttttat ttgcttgatg tctcttacaa
     1381 cctcacttat ttcccttctg tagtatttgt ttttgtaaag gcatgccaca gagcaaaagc
     1441 tgcaggaatg cgggcagcct cttcctgcct gcacaggaag aaagtttccg attttcttat
     1501 ccattatcaa atcaaacctg ggagttggat attttaaggt tgtaagaggt ttttcataat
     1561 aatccttcaa tgtgttattc tctatgtctc ttaaaacatc ttcccatact tcttccgcat
     1621 cgccaattat tacactatca cagtactttt tagcctcgtt gggcataagg cttgtcatat
     1681 agccacccat tagtacaatt ttccctcttt ttttaaactc ttttgcaata tcaatggacc
     1741 ttataactgc atgacccatg ctgctgattc caacaacatc gcaatcagtg tcaaaaggta
     1801 catcctctat tgtctcaaga cagatttcca cttgccagtc atcaggagta agggctgcaa
     1861 gaagagggag ggcaagacct acaaaataca gtcttgattt ttttatcagt tttccctgtg
     1921 aatcgtatgg tgttgattgt atgagtaaaa ttttcttttt catgtgtttc atccctttaa
     1981 aaatttttta aggtattgca tattcacttt gaaggcacct atgcttagtt tcaatgtatc
     2041 cataaggccg tactttttta tcatataaat gctctttttg ggattgacca tggtgctata
     2101 gtaaagaagt attgtataga gatagtactt tcttaccgat atctttgttg gccttacaac
     2161 aagatgtgcc atgtcccatt tttcatactc ctcccttttt acaataagtt tgtctttgta
     2221 caagtcatac atatcagtac caggcagagg cgtaaacggc tgcagattta caaaggtaat
     2281 tcccagtttt ttaatccatt ccttgagctt tttaaaatct tccctgtccc agtcaatccc
     2341 cagtatgaaa gtgccgtagc attcaatgtt atgttttttc agtattgcca ctgccttttc
     2401 attaatcttg ctgtttgatt ttttgttgta tccttcgagc attatgtcat cacaggattc
     2461 cagacctaca attaccgccc gaagtccgtt gtccttaagc cttttaacaa cttcctcgtt
     2521 ttcggcaatg aaatccgctc ttccgtaaac caggtatctt ttctttatgt tttctttttc
     2581 caacatgtcg cagaaatgat ttaatctatt tgcattgaaa agaaagttgt cgtccactat
     2641 gtagacttct tcttccttta ttgtttttag ttcattaatt atatcttcca cacttcttgc
     2701 aaagtatttg ttgtcagtga tttccctgca aaaacagaag ctgcagttgt agggacatcc
     2761 aaaggatgtt ttaatcagcg tacagtttct gtggaacatg tagtagtact tctttctata
     2821 cttttttact ttctcccggt caggaaaagg gtagcagaag gtttgtgatt tttctttttc
     2881 caat
//
LOCUS       DAONVY010000298         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23702_length_2880_cov_2.849912, whole genome shotgun sequence.
ACCESSION   DAONVY010000298 DAONVY010000000
VERSION     DAONVY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23702_length_2880_cov_2.849912"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLI11_07950"
     CDS             <1..560
                     /locus_tag="PLI11_07950"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPZ52837.1"
                     /translation="FYSLDRSFFIENDMKKYADYDSPIPIGYGQTISQPSLVLEMTKL
                     LSPDNDSKVLEIGTGSGFQTAILAKISKEVYTVERIKELMDKAQQRLKELNFTNVYFK
                     TGDGSKGWEEHALYDRIMVTCAARVLPKELVDQLKVGGRMVIPVGSPESQKLLLVTKT
                     DKGINMQDVGWVRFVELKGEYGWEQ"
     gene            complement(562..1554)
                     /locus_tag="PLI11_07955"
     CDS             complement(562..1554)
                     /locus_tag="PLI11_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52838.1"
                     /translation="MKRKLAMLLTLLLITSLAACDTAKNPYEGANSMQLIPDAQFSTG
                     FVVKSQKDHKNKDKVVDLGVFPYDKDKASARWSIAQWDSGPCLWENRKTDMPDNVLTN
                     GTDKKVEIKKGQVSLYLDSSGYYQGNPAIQGDYWPHLLIEQGSFAYKKLSNEEKIFYH
                     ADSEAMVLEIDIRLTEYEKTEIKGDWVRAAQLLMYFYVRSGKGDFLWFGLQLFDNRSD
                     NTEHYIGYDGGKADASGAMIYSIGSRHVYKNSKRTLWKNKSPHPSNEWIHVKINILPY
                     IEDAFNKGKEDGYFKAEDISELYIDGMNYGFETIGTFKHSADIKNLSLTSYRYD"
     gene            1854..2540
                     /locus_tag="PLI11_07960"
     CDS             1854..2540
                     /locus_tag="PLI11_07960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ52839.1"
                     /translation="MSKRNYGERARGGASWADAMWRLSAGSKNYVQGIFRRVIYKKNI
                     VCPDLSLELSSRSFKIKRGKTVFKMRYSEVERFRQEGAFKFQDFLENLLDNLIPDDLP
                     LFTMSFDDYDAVCNFWNKHSNITIKKESFREFLYENSKYSLICRDQEKIVGTLLCGQD
                     GLTGYIHNLTVDENYKDKGVEERLLEACLENLRENGIKDYMVFVNPDENSFWESCGWQ
                     KKEDIVVYSK"
     gene            complement(2563..>2880)
                     /locus_tag="PLI11_07965"
     CDS             complement(2563..>2880)
                     /locus_tag="PLI11_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPZ52840.1"
                     /translation="ESSVEIDETGMFVGVNGEYRVKNVYVGDEPLDLNKTYTVASHNY
                     MLQNMGDGYTMFSDNEFLQDNVMLDVQVLITYITKHLGGVIGEEYKEAQGRIIFVEKG
                     PGN"
BASE COUNT          846 a          536 c          610 g          888 t
ORIGIN      
        1 acttttactc attagacaga tctttcttta ttgaaaatga catgaaaaaa tatgccgatt
       61 atgattctcc tatacccata ggttatggac aaaccatctc gcaaccaagt cttgtgcttg
      121 aaatgaccaa gcttctgtct cctgataatg acagcaaggt tttggagatt ggaacgggtt
      181 caggttttca gactgccata cttgcaaaaa tatctaaaga agtctatacc gtagaaagaa
      241 taaaagagtt gatggacaaa gctcaacaga gacttaaaga actaaacttt acaaatgttt
      301 atttcaaaac aggggatgga agcaagggat gggaagaaca tgccctatat gacagaatta
      361 tggtgacttg tgcagcaagg gttctaccca aagagctggt agaccaactt aaggttggcg
      421 gcaggatggt cataccagta ggctctccgg agtcgcagaa gctgttgctt gtgacaaaga
      481 cggataaagg cataaacatg caggacgtgg gttgggtcag gtttgtggaa ctaaaaggag
      541 aatatggttg ggagcaatag cttaatcata cctgtatgat gtcaaagaca agttttttat
      601 atccgcactg tgtttgaagg tgcctattgt ctcaaaacca tagttcatgc cgtcaatata
      661 taattcactt atatcttctg ctttaaagta tccgtcttct tttcccttgt tgaaagcatc
      721 ttcaatatat ggaagaatat ttatttttac atgtatccac tcatttgaag ggtgaggtga
      781 tttattcttc caaagggttc ttttgctgtt tttgtacaca tgtctggaac ctattgaata
      841 aatcattgca cctgatgcat ctgcttttcc gccatcatag cctatgtagt gttcggtatt
      901 gtcgcttctg ttgtcaaaaa gctgcaatcc aaaccacaag aaatctcctt tgccgcttcg
      961 cacataaaaa tacataagaa gctgtgcagc tcttacccag tctcctttta tttcagtttt
     1021 ttcatattct gtaagacgga tatcaatctc aagaaccatg gcttcagaat cggcatgata
     1081 gaatatcttt tcctcattgc tgagcttttt gtatgcaaag gatccttgtt caatcagcag
     1141 atgcggccag tagtcgccct gtatggcagg atttccctgg tagtagccgg aagagtcaag
     1201 atacagagaa acctgaccct ttttaatctc cactttcttg tccgtgccat ttgtcagcac
     1261 attatcaggc atatcagttt ttctgttctc ccacagacag ggacctgaat cccattgggc
     1321 aatgctccaa cgggcagatg ctttatcctt gtcatagggg aatacaccca aatcaactac
     1381 tttgtccttg ttcttgtggt ccttctggct tttgactaca aagccggtac taaattgagc
     1441 atcaggtatc aactgcatgc tattggctcc ttcgtatgga ttttttgctg tatcacaggc
     1501 tgccaaagag gtaattaata gtagtgtgag aagcatggca agttttcttt tcataactat
     1561 cctcctgttt cttctacctg aattatatcg tttcagaaga aaattgtaaa tgaatggtgt
     1621 tgcatctttg gaaatattat gataatctat atccgacaaa ataaatattg tacttcttat
     1681 tacgagtcat ggtagagggt tagctcagtg accatgcacc aacctgcttt atggaaagca
     1741 aggtggtaat gctaacattg ataaggagtt ggaaagggct ccttgggagc tttttttgtt
     1801 ccttgatggc gactcaaacg gaagtacaaa agccatgaaa ggagattgat attatgtcaa
     1861 aaagaaatta tggagaaaga gcacgtggtg gtgcgagttg ggcagatgcc atgtggcgtc
     1921 taagtgcagg aagcaaaaac tatgtgcaag ggattttcag gagagttatt tacaagaaaa
     1981 atattgtgtg tcctgatttg tcgcttgaac tgtcgtcaag gtcattcaag atcaaaagag
     2041 gcaaaactgt atttaagatg agatattctg aagtggagag gttcaggcag gaaggagctt
     2101 tcaagtttca agacttcctt gagaatttgc ttgacaacct tataccggat gatttgccac
     2161 tctttacaat gagttttgat gattatgatg cggtttgtaa tttttggaat aaacacagca
     2221 atattaccat taaaaaagaa agctttagag agttcttgta cgaaaacagt aaatacagcc
     2281 tgatttgcag agaccaggag aaaatcgtag gaaccctact ctgtggtcag gatggcctta
     2341 caggatatat tcataatctg actgtggatg agaattacaa ggataaaggg gtagaggaaa
     2401 ggcttttaga agcatgcctt gaaaatctga gagaaaatgg cattaaggat tatatggttt
     2461 ttgtaaaccc tgatgaaaat tctttttggg agtcttgcgg atggcagaag aaagaagata
     2521 ttgtggtgta ttcaaagtga gtaaaacaaa ggacctggac ttttagtttc caggtccttt
     2581 ctctacaaag attattcttc cttgtgcctc tttgtactct tctccaataa ctcctcccaa
     2641 gtgcttggtt atgtatgtaa tcagcacctg gacgtcaagc atcacgttgt cctgcaaaaa
     2701 ttcattatca gaaaacatgg tataaccgtc gcccatgttt tgcagcatgt aattgtggct
     2761 tgccactgtg tatgttttat tcaagtcaag aggttcgtct cccacatata cattctttac
     2821 ccggtattct ccattaactc caacaaacat acctgtttca tctatttcca cacttgattc
//
LOCUS       DAONVY010000299         2877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23734_length_2877_cov_3.636074, whole genome shotgun sequence.
ACCESSION   DAONVY010000299 DAONVY010000000
VERSION     DAONVY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23734_length_2877_cov_3.636074"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLI11_07970"
     CDS             complement(<1..731)
                     /locus_tag="PLI11_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPZ52841.1"
                     /translation="MIQVQAHRGASAYAPENTLPAFELAVRMKADGVENDIHLTNDYR
                     YVVCHDDEIARTSTGTGKISENTYEYLNRFDYGTKFGKEYEGTKIPLLEEFMETVKDM
                     DVINIELKTPFNPQDRKKAFTILYDTIVSYKCLEKSMISSFDHNILKELKELFPKLKT
                     SLLYGHVLSPEETVAMVRTNNADFIHPALSCVNKAIVDECKRHGIGCNVWTVDSPEDI
                     QKAIEMEPTGIITNVPDRVLKALGRG"
     gene            complement(735..1994)
                     /locus_tag="PLI11_07975"
     CDS             complement(735..1994)
                     /locus_tag="PLI11_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPZ52842.1"
                     /translation="MEKVELLAPAGNMTKLKTAFLFGADACYLGGSQFGLRAKADNFT
                     LEEMKEAVEIANRLNKKIYVTVNILPRDDDMSQMLNYVRKLHGVGVHAVIISDIGFFN
                     LVREEIPQLDIHVSTQANNLNSQTIKFWAKNGATRVNLARELSLSEIGKINSTLLKDG
                     IDCELEAFVHGSMCMAYSGRCLISDFLADRPSNRGDCAQPCRWKYSLVEEKRPGEYMP
                     VEETEHGTFIFNSKDLCMVEHIDKMIESGISSLKIEGRMKSEFYTAVVVRAYRIAIDE
                     YYKNPTGYKNNVELHKELLGELSSVSHRNYSTGFYLGERGSQIYASASYIRDTQFIGT
                     IEKCDKIADNMYEIMFYLRGDFNRGDTLEFVAPGDGVFKVTINEMYDEEDNAIDRAAK
                     AMMVTRLRVPIFVAPGSVIRKRKAQED"
     gene            complement(2007..2633)
                     /locus_tag="PLI11_07980"
     CDS             complement(2007..2633)
                     /locus_tag="PLI11_07980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPZ52843.1"
                     /translation="MNDYLIGLITDRSDCLNKIYDEAVKNHIPIVREDMASLLKVIVH
                     MMKPGRILEAGTAIGYSSILMAEASSSDCIIDTVEIDPDLVVVARENIKRANLEERIH
                     VIAGDMQDVFACLDIKYDMIFLDGAKSRYIDVYDDILRLLKVGGVLLCDNISYHGKIY
                     DEPKFVDRKHRTIVANLRSFLERLTSDDRFVTSILETGDGIAISTRIK"
     gene            complement(2633..>2877)
                     /locus_tag="PLI11_07985"
     CDS             complement(2633..>2877)
                     /locus_tag="PLI11_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPZ52844.1"
                     /translation="YNETLDGDITEEHEKIQDPYNTYVAKGLPPGPICNPGLASIEAA
                     LKPEQHDYYYFVLRKDGSGGHIFSRTYEEHLKAKNS"
BASE COUNT          743 a          717 c          472 g          945 t
ORIGIN      
        1 cccctaccca gcgctttcaa aaccctgtcg ggcacattgg taattatacc cgtaggttcc
       61 atctctattg ctttctgtat gtcttcagga ctgtcaacag tccacacatt acatcctatt
      121 ccatgtctct tgcactcatc cactatagcc ttgttcacac aagataatgc cggatgaatg
      181 aagtctgcat tgtttgtcct aaccattgcc accgtctctt caggacttaa aacatgtcca
      241 tacagtagag aggttttaag ttttgggaac aactccttca gttctttgag aatattgtga
      301 tcgaagcttg aaatcatgga tttctcaaga cacttgtatg agactatagt gtcataaagg
      361 attgtgaaag ccttttttct gtcttgtggg ttgaaaggcg tttttaactc aatgtttatg
      421 acatccatat ccttcacagt ctccataaac tcctccaaca gaggaatttt cgttccctca
      481 tattctttcc caaactttgt tccgtaatca aaacggttta aatattcata tgtattttcc
      541 gagatctttc ctgtgcctgt tgaggttctt gcaatctcat catcgtgaca aaccacatat
      601 ctgtaatcat tagtcagatg aatatcgttc tcaacaccgt ccgccttcat tctgactgcc
      661 agttcaaaag caggcaatgt attttcaggc gcatatgcgg aagctcccct gtgtgcctgt
      721 acctgtatca tagattagtc ctcctgtgct tttctctttc ttattacact acccggtgca
      781 acaaaaattg gcacccttaa tcttgtcacc atcatggcct ttgcagccct gtcaattgcg
      841 ttatcttcct catcatacat ttcatttatt gttaccttaa aaactccgtc tccaggagct
      901 acaaattcca acgtatctcc cctgttaaaa tctcccctta aataaaacat gatttcgtac
      961 atattatcgg ctattttatc acacttttca atagttccaa taaattgtgt gtcacgaata
     1021 tatgaggcgc tggcataaat ctggctgccc ctctctccca gatagaatcc ggtggagtaa
     1081 ttcctgtgac ttacactgga gagttcccca agaagctcct tgtgaagttc cacattattc
     1141 ttgtaaccag taggattctt gtaatattcg tcaatcgcaa tcctgtaagc ccttacaacc
     1201 acagcggtat aaaattcact tttcatcctt ccctcaatct taagagaaga tattccactt
     1261 tctatcatct tgtctatatg ctcaaccatg cacaaatcct ttgagttgaa tatgaaggtt
     1321 ccatgctcag tttcctcaac aggcatatac tccccaggtc ttttttcttc aaccagactg
     1381 tacttccaac ggcaaggctg ggcgcagtct cccctgtttg aaggcctgtc cgcaaggaag
     1441 tcgctaatca aacatcttcc agagtaagcc atgcacatgc ttccatgaac aaatgcttct
     1501 agttcgcagt caatgccgtc ctttaacaga gttgagttta tcttcccaat ctctgataac
     1561 gacagctccc ttgccagatt tactctcgtc gccccattct ttgcccaaaa ctttatggtc
     1621 tgagaattaa gattgtttgc ctgcgtgctt acatgtatat caagttgcgg tatttcttct
     1681 cttaccagat tgaaaaaacc aatatcactg attatgacgg catgtactcc aactccatgc
     1741 agctttctta cataattcag catctgtgac atatcgtcat ctcttggtag tatatttacc
     1801 gtcacatata ttttcttatt caacctgttt gcaatttcca ccgcttcctt catttcttcc
     1861 aaagtgaagt tgtcggcttt tgctcttaaa ccaaactggc tgccaccaag gtagcaagca
     1921 tcagcaccaa acaaaaatgc cgttttcagt tttgtcatat tgcctgcagg agcaagcaat
     1981 tcaacttttt ccatctttat ctccttctat ttaatccttg tagatattgc tattccatcc
     2041 cctgtttcca aaatgcttgt aacaaatctg tcatctgatg taagtctctc cagaaaactg
     2101 cgcagatttg ccacaatagt tctatgtttt ctatcaacaa actttggttc gtcatatatt
     2161 tttccatggt aggatatgtt gtcacaaagc aaaaccccgc caaccttcag cagcctcaga
     2221 atatcatcat acacgtctat atacctgctt tttgctccat ctaggaaaat catgtcgtac
     2281 tttatgtcaa ggcatgcaaa cacatcctgc atgtcgcccg caattacatg aatcctttct
     2341 tccaggtttg cccgcttaat attctctcta gcaacaacca ctaggtcagg atcaatctca
     2401 acagtgtcga taatacagtc cgagctggat gcctctgcca taaggatgga agagtatcca
     2461 atggcagtgc ctgcctcaag tatcctgcca ggcttcatca tgtgaacaat aaccttgaga
     2521 aggcttgcca tatcctccct tactatgggt atgtgatttt ttactgcctc atcatatatc
     2581 ttattcaaac agtctgatct gtctgttatc aatccaatca aataatcgtt catcagctgt
     2641 tcttcgcctt caaatgttct tcgtaagttc ttgagaatat atgtcctccg gaaccgtctt
     2701 tccttagaac gaagtaatag tagtcgtgct gctctggttt tagagcagct tcaatggatg
     2761 caagaccagg attgcaaatc ggtcctggag gaagtccctt tgcaacgtat gtgttgtatg
     2821 gatcctgaat cttttcgtgt tcttctgtaa tgtctccatc cagagtttca ttatata
//
LOCUS       DAONVY010000300         2871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23792_length_2871_cov_3.949574, whole genome shotgun sequence.
ACCESSION   DAONVY010000300 DAONVY010000000
VERSION     DAONVY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23792_length_2871_cov_3.949574"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLI11_07990"
     CDS             <1..685
                     /locus_tag="PLI11_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPZ52845.1"
                     /translation="HVPINDMEALENAVNENTCAILLEVVQGESGVHPITKEYAQFAR
                     ELCDKHGIFLIIDEVQTGIGRTGKLFAYETVGIEPDIFTLAKALGNGFPIGAICAKEE
                     ASKGFQPGDHGTTFGGNPLACAAALAALSTIIEEEMPRKAEEMGKYLRDRIEGLNLDR
                     ISEIRNVGLMFAIEFKEPIAADVKNKMFEKGYLIGSVGANTLRILPPLIITESDVDGF
                     VDTLYNVLN"
     gene            705..1643
                     /gene="argF"
                     /locus_tag="PLI11_07995"
     CDS             705..1643
                     /gene="argF"
                     /locus_tag="PLI11_07995"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPZ52846.1"
                     /translation="MKHLLSLKDLTQEEILDIIDLAIKLKKDNKEGRTHHYLKGKTLG
                     MIFSKSSTRTRVSFEVGMYQLGGYALFLSQNDIQIGRGETIEDTAKVLSRYLDGIMIR
                     TFKQSDVEELARFGSIPVINGLTDDEHPCQVLADLQTIYEIKGTLKGLKFAYVGDGNN
                     MAHSLLHGCAITGMNIAVATPKGYECGQKFIDEAVEIAKKSGSVITITNDAIEAVRDA
                     DVVYTDTWASMGQEDEKEERKRIFMPYQVNKELFSHSKKDAIFMHCLPAYRGSEVTSD
                     VIDGEQSVVFDEAENRLHVQKAVMVRLMGNGLLVNN"
     gene            1621..2106
                     /locus_tag="PLI11_08000"
     CDS             1621..2106
                     /locus_tag="PLI11_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ52847.1"
                     /translation="MDYSLIIRRAKPTEEDVNAIRSVMIDAFEKYKLDTNLEGSMEAL
                     EETYEDIYNDIVNKYVYICFVDGVPVGSIRVDINKEKKIAYISRFGVCRGYQNIGIGK
                     SFMNLVDKLLISKGVKRAMLHAASKYTALVRFYYGCGFYIHSTSEDRGYIRALFYKDY
                     E"
     gene            2290..>2871
                     /locus_tag="PLI11_08005"
     CDS             2290..>2871
                     /locus_tag="PLI11_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52848.1"
                     /translation="MCKLRLINENMNELSFCILSRAVRRFLTERRCILSEEEGYAIKV
                     CVNADLKDDRYVINSYNTHAEIKAAKDCTVHAAVSRFFLESKFSGVGDFSPFVGQIDF
                     TPRSSVRGMYFATHFENFYHKAPVEKIYDIIEDLAFKGCNTLLVWYDMHHYDSIDQPE
                     SVEMIKRLKEILHYAQRIGIKAAMLMIANEAFAS"
BASE COUNT          907 a          458 c          696 g          810 t
ORIGIN      
        1 acatgtacca attaatgata tggaagcatt ggagaatgct gtgaatgaaa atacctgcgc
       61 aattttgctg gaagttgttc agggagaaag tggtgttcat cccattacaa aagaatatgc
      121 acaatttgca agagagcttt gcgacaaaca cggaatcttc ttgataattg acgaagttca
      181 aaccggtata ggtagaacag gcaaactgtt tgcatatgaa acagtaggta ttgaacccga
      241 tatatttact cttgcaaaag ccttgggcaa tggttttcct attggtgcca tttgcgcaaa
      301 agaagaagct tcaaaaggtt ttcagcctgg ggatcatgga acaacctttg gcggaaatcc
      361 tcttgcctgt gctgcagcgt tggctgcatt aagtacaata attgaggaag aaatgcccag
      421 gaaggctgaa gaaatgggca aataccttag agatagaatt gaaggattga atcttgaccg
      481 tatttcggaa attagaaatg ttggtcttat gtttgccata gagttcaagg aaccgattgc
      541 tgctgatgta aagaacaaaa tgtttgagaa gggttacttg ataggaagtg taggagcaaa
      601 tacattaagg atacttcctc cacttattat cactgaaagt gatgtggatg gttttgtgga
      661 tacgctttac aatgttctga attaatttgc aaagaaggtg cgctatgaaa catttgctta
      721 gtttgaagga tttaactcag gaagagatac tggatattat tgatttggca ataaagttga
      781 agaaggataa caaggaaggc aggacccacc actacctaaa gggaaagact ttgggaatga
      841 ttttttcaaa gtcttctaca agaacaaggg tttcctttga ggttggaatg tatcagctcg
      901 gtgggtatgc tttgttttta agtcagaacg acatccagat aggcagggga gaaaccattg
      961 aagatactgc aaaagttctt tcaagatatc ttgatggtat tatgatcagg acattcaaac
     1021 agtcagatgt ggaagaactg gcaaggtttg gctccatccc agttattaat gggcttactg
     1081 atgacgaaca tccctgccag gttcttgcgg atctgcaaac aatctacgag ataaaaggaa
     1141 ctttaaaagg tctgaagttt gcatacgttg gagatggaaa caacatggct cattctcttt
     1201 tgcatggctg tgccataact ggaatgaaca tagctgtggc aacacccaaa ggttatgagt
     1261 gcggacagaa atttatcgat gaggctgttg agattgcaaa gaaatccggt tccgtaatca
     1321 ccatcaccaa tgatgcaatt gaggctgtca gggatgctga tgtggtttac actgacacat
     1381 gggcaagcat ggggcaggag gatgaaaaag aagagagaaa gaggattttc atgccttacc
     1441 aggtaaacaa agaactcttt tcacacagca agaaggatgc gatcttcatg cactgcctgc
     1501 ctgcataccg gggtagcgag gttacttctg atgtcattga tggagaacag tctgtagtat
     1561 ttgatgaggc tgaaaacagg ctgcatgtac aaaaagctgt aatggttagg ttgatgggaa
     1621 atggattact cgttaataat tagaagggca aaaccaacag aggaagatgt aaatgcaatc
     1681 aggtctgtaa tgattgatgc ttttgaaaag tacaagcttg acacaaatct tgagggaagc
     1741 atggaagcgt tggaagagac gtatgaagac atatacaacg atattgtcaa caagtatgtc
     1801 tatatctgct ttgtggatgg agttccggtt ggcagcatcc gggtggatat taataaagag
     1861 aagaagattg catatattag caggtttggt gtttgcagag ggtaccagaa tattggtata
     1921 ggcaagtctt ttatgaatct ggtggacaaa ctcctaattt caaagggagt aaagagggca
     1981 atgctgcatg cagcttccaa gtataccgcc ttggtcagat tctattatgg ctgcgggttt
     2041 tacatccatt caaccagcga agacagagga tacataagag ctttgtttta caaggattat
     2101 gagtaagaaa atctaaaaca ggctcatgga tatgttattt atccaattat ataaatcaat
     2161 ccctaaaaac ctaccctccc acatgggggt gggttttgct ttatgctagt tttgatattg
     2221 ttgttaattt gggttgcgat tgatataatg ttttaaaata caagaattta ccggggtaca
     2281 aggagaggaa tgtgtaaatt aagattgatt aatgaaaata tgaacgaatt aagcttttgt
     2341 atcctatcaa gagcggtacg aaggttcttg acggaacgca ggtgcattct ttctgaagaa
     2401 gagggatatg caattaaggt ttgtgtcaat gccgatttaa aggatgacag gtatgttatt
     2461 aactcataca acacgcatgc agaaataaaa gcggcaaagg actgcacggt ccatgcagca
     2521 gtttcccgtt tctttttgga aagcaaattt agtggtgtgg gtgacttttc tccatttgtc
     2581 ggtcaaattg attttactcc cagaagttct gttcgcggaa tgtattttgc aacccatttt
     2641 gaaaactttt atcacaaggc acctgttgaa aaaatatatg acataattga ggatcttgct
     2701 tttaagggct gcaacacttt gcttgtctgg tatgacatgc accattatga ctcgattgac
     2761 cagccagagt ctgtggaaat gattaagaga cttaaagaaa tattgcacta tgcacaaagg
     2821 attggaatca aggcggcaat gctcatgata gccaatgaag cttttgcgtc t
//
LOCUS       DAONVY010000301         2869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23815_length_2869_cov_2.485785, whole genome shotgun sequence.
ACCESSION   DAONVY010000301 DAONVY010000000
VERSION     DAONVY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23815_length_2869_cov_2.485785"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLI11_08010"
     CDS             complement(<1..943)
                     /locus_tag="PLI11_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ52849.1"
                     /translation="MKVLIVGGVAGGASCAARLRRLDESAEIIMFERGEYISYANCGL
                     PYYIGEKIKRKSDLTLQTPESFKRRFNVDVRILNEVVPINREDKKVVVKDIRTNEMYE
                     ESYDKLVLSMGAKPFKPPIKGIDLPNCFTLRTVPDTFRIKEYIQNNNPKSAVVVGGGY
                     IGIEMAENLKEAGLDVILIELSDHLLPPLDFDMACDVHKYVRQNGINLMLNNGVREIE
                     EIEDGLLVKLDKCKIQTDMVILCVGVRPESDIAKEANLQINERGSIIVNDNMLTSDPD
                     IYAVGDAVVVTNFITKKDAFIPLASPANKQGRIAANNI"
     gene            1178..>2869
                     /locus_tag="PLI11_08015"
     CDS             1178..>2869
                     /locus_tag="PLI11_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPZ52850.1"
                     /translation="MIFYTAADEERPIRLSEATRLFAYESMNGKYGDDAMKNTAVALD
                     HIEGFENLSDIDKYDIAIREIAEKAPIRVCEYELVHGSASLGLSINHCIPATYKGSPI
                     FHSVSHLTSGFDRVVYEGIDSYEERIHKKLENESLTARQIRFLKSLLNVIESMRIWHK
                     RYSEVIEMGDMPFKKAENFKEAVQSLWFTFAFMRLCGDWPGIGRIDKILGPYLKKDLD
                     QGVLTLDEAREILASFFIKGCEWIRGETPLGSGDAQHYQNIILAGVDEDNKEVANEVT
                     YLVLDIVEELGISDFPITVRVNENTDERLLKRVAEVIRHGGGIVAVYNEPLILNALEK
                     LGYDPKEARKFANDGCWEVQIPGETYFCYVPFDALQILLTKTLNLNGEYKTFDSFEDL
                     YNCFIENLRQDVNAIYEHVVMGRFRKTEKGYEYKEAVPCSVTSLFIKGCIEKASSYYE
                     GGPIYTVVSPHIGGAPDVGNSLYAIKKLVFDDKIISFDELMKALKANWEGYEVIRQIA
                     LNRYVYYGNDDENGDYYTVKVLDDFADIVNAVEKKVDILFPAGISTFGRQIEWAPF"
BASE COUNT          836 a          518 c          635 g          880 t
ORIGIN      
        1 aaatgttatt tgctgcaatc ctgccttgct tgtttgcagg actagccaaa ggaataaagg
       61 cgtctttttt ggtgatgaag tttgtaacaa ccaccgcatc tcctacagca tatatgtccg
      121 gatcgcttgt cagcatgttg tcgtttacaa taattgaacc tctttcattg atttgcagat
      181 ttgcttcctt tgctatatcc gactcgggtc ttactcctac gcaaagtata accatgtcag
      241 tttggatttt gcacttgtca agtttaacaa gaagaccgtc ttcaatttct tctatttccc
      301 tgacaccgtt gttcagcatg aggttgattc cattttgcct tacatatttg tgtacatcgc
      361 aggccatgtc aaagtccaga gggggtagca aatggtcgga aagttcgata agaatgacat
      421 caagaccagc ttcctttaaa ttctccgcca tctcaatgcc aatgtatccg ccgcctacaa
      481 caacagcgga ttttgggtta ttattctgga tatattcttt tattctaaag gtgtcgggaa
      541 cagtccttag tgtaaagcaa ttgggcaaat caataccttt tatgggtggt ttgaagggtt
      601 ttgcacccat ggaaagtacc agcttgtcat aggattcctc atacatttcg ttggttctta
      661 tatcctttac aaccaccttt ttatcttctc tgttgattgg cacaacttca tttaaaatcc
      721 ttacatccac attgaatctt cttttgaaac tctcgggtgt ctgcagtgta aggtcggatt
      781 tcctttttat tttctctcca atgtaatagg gaagaccaca gtttgcatag gaaatgtatt
      841 cgccgcgttc gaacatgatt atctcggcac tctcatcaag tcttcttaac cttgccgcac
      901 aggaggctcc tcctgcaaca ccgccaacta tcaatacctt cataaacaat cctccatcct
      961 tctttttcta tattataccc ccatcattat tttcatggca actgcctgcc tttttcgtta
     1021 ggcaggcagc aatatatatg atctattaat ggaggataca tatataaata tgaaaagagc
     1081 agttccacag ctattatatg tacatatcaa aaaaagttct gtgacaatgt cacttttctg
     1141 tgataatata tagtggaaga tttaaaaggt ggtatttatg atattctata ctgcagcgga
     1201 tgaggaaaga cctataagac ttagtgaagc aaccagatta tttgcatatg aatccatgaa
     1261 tggcaaatat ggcgacgatg ccatgaaaaa tacagcggtt gcccttgatc atatagaggg
     1321 gtttgagaac ttgtctgata tagacaagta tgatattgca ataagggaaa ttgcagaaaa
     1381 agcaccaata agggtatgtg agtacgaact tgtacacgga agcgcatccc tggggctgtc
     1441 aattaatcac tgcatacctg caacctacaa aggttctccg atctttcata gtgtaagcca
     1501 tctgacttcc ggttttgaca gggtggttta cgaaggcatt gattcctatg aagaaaggat
     1561 acataagaaa ctcgagaatg agagcttaac ggcaagacag attcggtttc tcaagagtct
     1621 tttgaatgta attgaatcaa tgagaatatg gcacaagcgg tattcagagg ttattgaaat
     1681 gggcgatatg cctttcaaaa aagcggagaa tttcaaggaa gctgttcaaa gtttgtggtt
     1741 tacctttgct ttcatgcgcc tttgcggaga ctggcctggt attggaagaa tagataaaat
     1801 attgggtcct tatctaaaaa aggatcttga tcaaggtgtt ttaactcttg atgaagccag
     1861 agaaatactt gcttcctttt tcataaaagg gtgtgaatgg attagaggag aaacaccatt
     1921 gggttcggga gatgcccagc attaccagaa cattattctt gcaggagtgg atgaggataa
     1981 taaagaggtt gcgaatgaag ttacatatct tgttcttgat attgtagagg agcttgggat
     2041 aagtgatttt cccataacgg tcagggttaa tgaaaatacg gatgaaaggc ttcttaaaag
     2101 agtggcggaa gtaattcgtc atggtggcgg tattgttgcg gtatacaatg agcccttaat
     2161 attaaatgct cttgaaaaat tgggatatga cccaaaagag gcaagaaagt ttgctaacga
     2221 tggatgctgg gaagtacaaa tcccaggtga gacgtacttt tgctatgtgc cctttgatgc
     2281 cttgcagata cttctgacaa aaacactaaa tttgaatgga gagtacaaga cctttgacag
     2341 ttttgaagat ttgtacaact gtttcataga gaatctcagg caggatgtaa atgcaattta
     2401 cgagcatgtg gtaatgggaa ggtttagaaa aacggagaaa ggatatgagt acaaggaagc
     2461 tgttccttgt tccgtaactt ctttgtttat aaaaggctgc attgaaaagg cctcttcata
     2521 ttacgaagga ggtccgatat acactgtcgt gtctccgcat attggaggag ctcccgatgt
     2581 tggaaacagt ctgtatgcca ttaagaaatt ggtgtttgat gacaagatca tttcctttga
     2641 tgaacttatg aaggcgctta aggcaaattg ggagggatat gaggttatcc gccagattgc
     2701 tcttaacagg tatgtctact atggcaatga tgacgaaaat ggagactatt atacagtcaa
     2761 agtgcttgat gattttgctg atattgtaaa tgcggtggaa aagaaggtgg atattttgtt
     2821 ccctgcaggg atcagcacct ttggtagaca gattgagtgg gctccgttc
//
LOCUS       DAONVY010000302         2868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23823_length_2868_cov_3.186989, whole genome shotgun sequence.
ACCESSION   DAONVY010000302 DAONVY010000000
VERSION     DAONVY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23823_length_2868_cov_3.186989"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..938
                     /locus_tag="PLI11_08020"
     CDS             93..938
                     /locus_tag="PLI11_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPZ52851.1"
                     /translation="MDKQKALELKKTATRIRKHIIEQVFSAKSGHPGGSLSCTDILTV
                     LYFDEMRVNPKDPRWADRDRFVLSKGHCAPALYATIALKGFLPEEDLKTFRKIDSYLE
                     GHPSMSYVPGVDMSTGSLGQGISTAVGMALAGKIDNKDYRVYSILGDGELQEGQVWEA
                     AMAAAHYKLDNLTAFVDYNGLQIDGNITDVMNPEPIADKFKAFGWNVIVVEDGHDHAE
                     IKAAIEKAKTVKGQPTMCVCHCIKGKGVSFMENDYSWHGTAPNQEQRDKAIAELDEYL
                     AGLEA"
     gene            943..1878
                     /locus_tag="PLI11_08025"
     CDS             943..1878
                     /locus_tag="PLI11_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HPZ52852.1"
                     /translation="MAKVATREAYGKALAQYGADERIIVLDADLSKSTKTDTFRQVYP
                     ERHFNMGIAEGNLMCVAAGLASTGKVVFASTFAIFASERACEQIRNSICYPKLNVKVA
                     ATHAGLTVGEDGASHQCVEDLGVMRSLPNMVVLSPCDEIETNRAIKAAIEHDGPVYIR
                     LGRLAVEAVHDPDNFEFEIGKGIEVREGNDVTLVATGLMVQESLAAAKILEENGINAR
                     VIDMHTIKPVDKEILVKAAKETGFVVTVEEHNIVGGLGSAVCEALSENYPCRVLRIGV
                     EDKFGKSGTPAALLEKYGLNAEAIAKKTMEAMKKK"
     gene            1933..2808
                     /locus_tag="PLI11_08030"
     CDS             1933..2808
                     /locus_tag="PLI11_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPZ52853.1"
                     /translation="MKRICLSFAILLAVMCLFVSNSEEAESKNLTSMCDIKVSGGQSV
                     DVIVDGSQYGSEWYQSGTVFEIECEEEIAGIYIIWDRKAPIWTMESDGNTYTYGEKEF
                     LHEFVEVENTKKLKITLSESARICEIYVFGEGILPNWVQVWEESHDKADLLLLSTHAD
                     DEHLFFAGVLPYYAVERGLKVQVVYMTNHNDTQSRPHELLNGLWVVGIRHYPVISDFP
                     DLAGSLGQKGEDVETVLKRALILYDEEEVTRFHVENIRRFKPKVIVGHDINGEYKHGA
                     HMVNAFTLMKALELS"
BASE COUNT          886 a          489 c          736 g          757 t
ORIGIN      
        1 atgagggtgg acgtcatgca agaagagttg gcatgatttc cgacatagaa gagaaatata
       61 tgaaataaat tatttatgaa ggagtgtagt atatggacaa gcaaaaagcg ctggagctaa
      121 aaaagacagc gaccagaatc agaaaacata taattgagca agtgttttct gcaaagtcag
      181 gtcatccagg tggttcatta tcatgtaccg atatattgac agtactgtat tttgatgaaa
      241 tgagggtcaa tccaaaagat ccgaggtggg cggacagaga ccgttttgtt ttgtcaaaag
      301 gtcactgcgc acctgcactt tatgcaacaa ttgcattgaa aggattttta cctgaagaag
      361 atttgaagac cttccgtaaa attgacagtt atctcgaggg tcacccaagc atgagctatg
      421 ttccaggtgt tgacatgtcc actggttctc ttggacaggg catttcaaca gcagtaggaa
      481 tggcgctggc aggcaaaatt gacaacaagg attacagggt ttactcaata ttgggtgatg
      541 gtgaacttca ggaaggacaa gtttgggaag cggctatggc tgcagctcat tacaaactcg
      601 ataatttgac cgcttttgtt gattacaacg gtctgcagat agatggaaat attacagatg
      661 taatgaatcc tgaacctata gctgacaagt tcaaggcatt cggctggaat gttattgttg
      721 tggaagacgg acatgaccac gctgaaatca aggcagctat tgaaaaggct aaaacagtaa
      781 aaggacaacc tacaatgtgc gtatgccatt gtattaaagg taagggtgtt tcttttatgg
      841 agaacgacta cagctggcat ggaactgcgc ctaaccagga gcagagagac aaagctatag
      901 ctgaattgga cgaatacctg gcaggattgg aggcttaaga atatggcaaa agtagcgact
      961 agagaagcat atggcaaggc gttggctcag tacggtgcgg atgagagaat tattgttctt
     1021 gatgccgacc tttcaaagtc aactaaaaca gatacattta gacaggttta tcctgaaaga
     1081 catttcaaca tgggaattgc agaaggcaac cttatgtgcg ttgcagcagg tcttgcatcc
     1141 acaggcaaag tagtgtttgc cagcacattt gcaatctttg catcggaaag agcgtgtgag
     1201 cagattagaa actccatctg ctatcccaaa ttgaatgtaa aggtggcggc aacccatgca
     1261 ggtcttactg ttggagaaga cggagcatcc catcagtgcg ttgaagactt gggagtaatg
     1321 agatctcttc caaacatggt agtacttagc ccctgtgacg agatagaaac aaaccgtgca
     1381 atcaaggcgg ctattgagca tgacggccct gtatatataa ggcttggaag acttgctgta
     1441 gaggctgttc atgatcctga caattttgag tttgaaattg gtaaaggcat tgaagttagg
     1501 gaaggaaatg acgtaacttt ggtggcgaca ggactcatgg ttcaggaatc cttggcagca
     1561 gctaagattc ttgaggagaa tggaatcaat gcaagagtaa tcgacatgca cacaatcaag
     1621 cctgtcgaca aggagattct tgttaaagca gcaaaagaaa ccggatttgt tgtaactgtt
     1681 gaagagcaca acattgtagg cggccttggc agcgcagtgt gcgaggcgct ttccgagaac
     1741 tatccttgca gagttctgag aattggtgtt gaagacaagt ttggcaagtc tggcacacct
     1801 gctgctctgc ttgaaaaata cggtctgaat gcagaagcta ttgccaagaa gaccatggaa
     1861 gcaatgaaaa aaaagtaaca aaaacaaacc ggagtgatta aaaacctccg gttctttttt
     1921 atggaggttc gaatgaaacg tatctgtttg tcttttgcaa ttcttttggc agtcatgtgt
     1981 ctgtttgttt caaacagtga ggaagcagaa agcaagaacc ttacctcgat gtgcgacatt
     2041 aaggtatctg gtggccaatc tgttgatgta attgtagatg gaagccagta tggctctgaa
     2101 tggtatcaaa gcggtaccgt gtttgagatt gagtgcgaag aagaaattgc tggcatatac
     2161 ataatatggg acaggaaggc gccgatatgg acaatggaat cagatggcaa tacctacaca
     2221 tatggggaga aggaattttt gcatgaattt gtagaagttg aaaatacaaa gaaattaaag
     2281 attactcttt ctgaaagtgc aaggatttgt gaaatatatg tgtttggaga aggaattttg
     2341 cccaattggg tgcaggtttg ggaagaatct catgataagg cggaccttct tcttttgtct
     2401 acccatgctg atgacgagca tttgtttttc gcaggagtcc ttccatatta cgctgtggaa
     2461 agaggtctca aagtccaagt tgtgtatatg accaaccata acgatacgca gtcaaggcct
     2521 catgagcttt tgaatggtct ttgggtggtg gggataaggc actatcctgt aatatctgat
     2581 tttcctgatc tggcaggttc tttagggcaa aagggagagg atgtggagac tgtacttaaa
     2641 agagctctaa ttctgtacga tgaagaagaa gtaacgaggt ttcatgtgga gaatatcaga
     2701 aggttcaaac caaaagttat tgttggacat gacataaatg gtgagtacaa acatggggcc
     2761 catatggtaa atgcgttcac tcttatgaaa gctcttgaac tttcataaga gtgaacgcat
     2821 ttaccatatg ggccccatgt ttgtactcac cagttattgt tggacatg
//
LOCUS       DAONVY010000303         2864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23854_length_2864_cov_4.118548, whole genome shotgun sequence.
ACCESSION   DAONVY010000303 DAONVY010000000
VERSION     DAONVY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23854_length_2864_cov_4.118548"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2458)
                     /gene="addA"
                     /locus_tag="PLI11_08035"
     CDS             complement(<1..2458)
                     /gene="addA"
                     /locus_tag="PLI11_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HPZ52854.1"
                     /translation="MAWTDEQLEAINHRGKNLLVAAAAGSGKTSVLVERIIKRILDDN
                     IDVDKLLIVTFTRDAASEMKERIYASIQSRLKDSDNPRRLLEQQALLSKAHISTISSF
                     CQYVVKSNFRESGIDSSFRVADDNETLLIKEEVLDDVLEKWYEKKDPHFHNLVETYGG
                     SKTDDNLKSIILNLHSFIQSFPYPFEWLDEQKKILNPKRYKDFGETIWGKEALEYLEI
                     TLESLLSEAKRTKAMAEEFGVSGYLDALGIDVEMLGDLCSMCKNASWEEAFEIMNSFS
                     FVRLGRVGKEDDREKAVLIKKRRDTLKKELGKLVSEIFGGSKDAAMKDMELVYKDIRR
                     LVWLVKYFHLKFQNAKKERKIFDFSDMDHIALNILAKKENGRIVPTDVALKLREKFEE
                     IYIDEYQDTNLTQETILQVIARGNNIFHVGDVKQSIYSFRQARPDLFLNKYHEYSVNT
                     EKNKLVRLYKNFRSRRDVVGSVNYIFSRIMNEKTAEMDYVESEYLNYGATYYDDAYNS
                     ECELLIIENKDSTKENTYLEAMLIASKIRKLFEDGFMVYDKREKTMRKIQYKDIVILM
                     RSVSEKADIFRQVFASCGIPLYYEGKGGFFASLEISVVLAFLSLIDNPLQDIPLITVL
                     RSPMFEFSDAEISLIRLHKKDCLFYEALLEYAESGYAESLREKINEFLNVLGMLRDKS
                     VISSTAELVWDIVTITGYYTYVGLMEDGGRKQANLRLLFEKAASFDEGSHKGIYRFLN
                     YMENLKKKKGDLSEASAISEGMDVVRIMTIHKSKGLEFPVVFLANTAQEFNTQDVKGR
                     FVRHKTLGIGPSCFDRENMVV"
     gene            complement(2465..>2864)
                     /locus_tag="PLI11_08040"
     CDS             complement(2465..>2864)
                     /locus_tag="PLI11_08040"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPZ52855.1"
                     /translation="DHSMSNRERADYLNKYDCNLVISISCDSSNNPMDNGVQVYIPRF
                     TTSESTKVFAETLLNKLSNGLGVPKSGVKVANDITFLNWSKGNVVKVEIGYLSNREDE
                     RKLTDESYQLLYANYIKESIVEVLLQKNQE"
BASE COUNT          791 a          685 c          466 g          922 t
ORIGIN      
        1 aaacaaccat gttttccctg tcaaaacacg aaggaccaat gcccaaagtc ttgtgcctca
       61 cgaatctccc ctttacatcc tgggtattga attcctgagc ggtgtttgca aggaacacta
      121 caggaaattc cagacctttg cttttatgta tggtcataat tctcaccacg tccataccct
      181 ctgaaattgc ggatgcttcg gaaagatctc cttttttctt cttcaggttc tccatataat
      241 taagaaatct gtaaattcct ttatgagaac cttcatcaaa gcttgcagcc ttctcaaaca
      301 aaagcctaag gtttgcctgt tttctcccgc catcctccat taaccccaca taagtgtagt
      361 accctgttat ggtgacaata tcccaaacaa gctcagccgt cgaggaaatg acactcttgt
      421 ctctcagcat gccaagaaca ttcaaaaact catttatttt ctccctcaag gactcagcat
      481 atcccgattc cgcatactca agaagagctt cataaaacag acaatccttc ttatggaggc
      541 gaatcaggga tatttcagca tcggaaaact caaacatggg agaacgcaaa actgtaataa
      601 gggggatgtc ctgaagagga ttgtcaatta agcttaagaa agccaaaaca acacttattt
      661 caagacttgc aaaaaatcca ccttttcctt cgtagtacaa ggggatgcca caacttgcga
      721 aaacctgtct gaatatgtca gccttttctg acaccgatct catcaatatg acaatatcct
      781 tgtattgaat cttccgcatg gtcttttcac gcttgtcata gaccataaaa ccatcctcaa
      841 agagctttcg gattttgctt gctatcagca tggcttcaag atatgtgttt tctttggtgg
      901 agtccttgtt ttcaattatc aaaagctcgc attccgagtt gtacgcatca tcgtaatatg
      961 tggcaccata gttcaagtac tcactctcta cataatccat ctctgcagtc ttttcattca
     1021 taatcctgct gaatatatag tttacactgc caacaacatc tcttctgctt cggaagtttt
     1081 tatacaatct gacaagtttg ttcttttccg tatttacact gtattcgtgg tacttgttca
     1141 agaataaatc aggacgcgcc tgcctgaaac tgtatatgct ctgctttacg tctcccacat
     1201 ggaaaatatt attccctctg gctattacct gcagaattgt ttcctgggtc agattcgtat
     1261 cctggtattc atctatgtat atttcctcaa acttctccct taatttaagg gcaacatctg
     1321 taggaactat tctgccattc tcttttttgg caagtatgtt aagagcaata tgatccatat
     1381 cggaaaagtc aaatattttt ctttccttct tggcgttctg aaactttaaa tgaaaatact
     1441 ttactagcca aaccagcctg cggatatcct tgtacacaag ctccatatct ttcatggctg
     1501 catccttaga gccaccaaat atttcgctta caagcttgcc caactccttt ttcagtgtgt
     1561 cccttctttt ctttatcaga accgcctttt ccctgtcgtc ttctttgccc actcttccca
     1621 gtctgacaaa ggaaaaagaa ttcattatct caaatgcctc ttcccaagaa gcatttttac
     1681 acatgctgca caaatcaccc agcatctcaa catcaatacc aagagcatca agataaccgg
     1741 aaacaccgaa ttcttcggcc atggcttttg tcctctttgc ttcggacaaa agactttcca
     1801 gggtgatctc aagatactca agagcttcct ttccccatat ggtttctccg aaatccttgt
     1861 atcttttggg attcagtatc ttcttctgct catcaagcca ttcaaaggga taaggaaaac
     1921 tttgtatgaa agaatgaaga ttaagaatta tgctttttag attgtcatct gtttttgaac
     1981 cgccgtatgt ttcgaccaga ttatgaaaat gagggtcctt cttttcgtac catttttcaa
     2041 gaacatcatc gagaacctcc tccttaatca aaagggtttc attgtcatcc gccaccctga
     2101 aggaagagtc tattccgctt tccctgaaat tgcttttaac aacatattga caaaaagaac
     2161 ttatggttga tatgtgagcc ttgctcagaa gagcctgctg ctctagaagt cttctcggat
     2221 tgtctgaatc ttttaatctt gattgtatgg atgcatatat tctttccttc atttcagaag
     2281 ccgcatctct tgtgaaggtg acaattaaaa gcttgtccac atcaatattg tcatccaata
     2341 ttcttttaat aattctttcc actaatacag atgtcttacc agagcctgcg gcagccgcaa
     2401 ccaacagatt tttacctcta tgatttatcg cttccaactg ttcgtctgtc caagccatta
     2461 acaattactc ctgatttttt tgtaataaca cttcaacaat ggattcctta atgtaattag
     2521 cgtatagcag ctggtaactt tcgtcagtca attttctctc gtcttcccta ttagacaaat
     2581 acccaatttc caccttgaca acattgccct tagaccagtt caaaaacgta atgtcatttg
     2641 caaccttaac accagacttt ggaacaccaa ggccatttga gagtttgttc agcaaggttt
     2701 ctgcaaaaac cttggttgat tcgctggtgg taaatctcgg aatatatacc tgcacgccgt
     2761 tatccatagg attattggat gagtcacagc ttatgcttat aacgagattg cagtcgtatt
     2821 tgttcaaata gtccgccctt tcgcggttgc tcatggaatg atca
//
LOCUS       DAONVY010000304         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_23944_length_2856_cov_4.115673, whole genome shotgun sequence.
ACCESSION   DAONVY010000304 DAONVY010000000
VERSION     DAONVY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23944_length_2856_cov_4.115673"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="PLI11_08045"
     CDS             complement(<1..1935)
                     /locus_tag="PLI11_08045"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52856.1"
                     /translation="MAFLQWEKKLHAHYCIRVKFETEKEKVRLNMTGRTQYIFYLDGE
                     FAGDGPTRFHYTNPQYTEYFLDMEKGMHVITVILEYQDLATRICDNQLPPFFMIEIYG
                     EDGREIEPEIAYANLGGFDQSGSIKINAILGFMEWRDTSFTPQNIFNLDFNDSSWERR
                     YSKERLFDVELDKTEPAKNLEIEMTRIGSGYLTEEFGYEKDFPQARFSLRRLRHLVSP
                     PQGIWRRYDLGTVKLVRPCITIDCPKGATVEFALCESLVEGGRVTPFVSLTTGQSANM
                     VHFTARGGVETFMPITPLGGRYLEVHVVCNPSEVDNIEILEEKAYWRTFFDEKVGEFE
                     CNDGRLNVIWQMGVDTLRSCTEDVVTDCPVRERGQWTGDSAVVGLRTASVAYGDLQVI
                     RKSLLQASTLTDEDGVIPALYPGQIAYFITYSLLWVAGLRDYYDYCGDDTLLREVNKP
                     LKRFMNFMEKSLDFNDGLLKKNGIELSCGFIDWGYEVIPGRMIVPVICMYYTALNAYL
                     EISDVLGEDDAKDKIIRQIENVRMLLERAKKSMEPEFHSYVMLLYMGMFKEEERQPVL
                     EYIKNFITSCFPNNPDAPRLYSPTIFLKQIITPFFMNFALTALMENGEDEFATRQIKS
                     CWGYMLDLGETTCLEVFDTRW"
     gene            2182..>2856
                     /locus_tag="PLI11_08050"
     CDS             2182..>2856
                     /locus_tag="PLI11_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPZ52857.1"
                     /translation="MRQKSIFNNIYHVINIGVGDMIIFRNEEDKDYFCQLVQKNVQKH
                     SITIYAYAIMDNHFHFCLSSPDFNNISNFMRDVCAPYARYFNRTEITMKNNKPTKRHG
                     AVFAEAFKAIPVNSLNQMVTLVNYIHNNPCSAKEDPKTYVYSSFTKYLNILYKIKNEK
                     AAEKKWSIYINTSYFRELSYKEFIKLMTRDENDRFCEGDSELNGKYYMSDTMVIKEVE
                     NVFKMKI"
BASE COUNT          860 a          643 c          486 g          867 t
ORIGIN      
        1 ccatctggta tcaaaaactt ccaggcatgt ggtctctccc agatccaaca tgtatcccca
       61 acaggattta atctgccttg ttgcaaactc gtcctcacca ttctccatga gggcggtcag
      121 cgcaaaattc ataaagaacg gagtaataat ttgctttagg aatatggtag gactatacaa
      181 ccttggcgca tccggattgt ttggaaaaca gctggttata aagttcttga tgtattccaa
      241 aacaggctgc ctctcctctt ctttaaacat tcccatataa agaagcataa cataggagtg
      301 aaattccggc tccatggatt tcttggctct ttcaagcagc atgcgtacat tttctatctg
      361 tctgataatt ttgtcttttg catcatcttc tcccagtaca tcagagattt ccaaatatgc
      421 attaagcgca gtatagtaca tacagattac tggcacgatc atcctgccag gtattacttc
      481 ataaccccag tcaataaagc cgcaacttag ctcaatgccg tttttcttta acagaccatc
      541 attaaaatca aggctctttt ccatgaaatt cataaaacgc ttaagcggtt tgtttacttc
      601 ccggagtaaa gtatcatccc cgcagtagtc gtagtagtcc ctaagacccg caacccagag
      661 caaagaatat gttatgaaat atgcaatctg acccggatac agagctggta ttactccatc
      721 ttcatctgtc agtgttgaag cctgcaataa agactttctg attacctgca agtctccata
      781 agctactgat gcagttctta atcccacaac agcactgtca cctgtccatt gacctctttc
      841 cctaacagga cagtcagtaa ccacatcctc agtacaagag cgtaacgtat ctacacccat
      901 ctgccagatg acatttagcc ttccgtcatt gcattcaaat tctcctactt tttcatcaaa
      961 gaaggttctc cagtacgcct tctcctcgag tatttctata ttatctactt ccgatggatt
     1021 acacaccaca tgaacttcaa gataacgccc tccaagagga gttattggca tgaaagtctc
     1081 aacaccacct ctggcagtga aatgaaccat gttggcagat tgaccagttg tcaaactcac
     1141 aaaaggtgta accctgcctc cctctaccag tgattcacac aatgcaaatt ccacggtagc
     1201 accttttggg caatcaattg taatgcatgg acgaacaagc tttactgtac caaggtcata
     1261 tcttctccag atgccctgcg gaggacttac caggtgtcta agtcttctca gtgaaaatct
     1321 tgcctgcgga aagtcttttt catatccgaa ttcttctgtc aggtacccgc ttcctattct
     1381 cgtcatttct atctccaagt tcttggctgg ctctgtttta tccagttcaa catcaaacag
     1441 gcgttctttt gaatatctcc tctcccagga gctgtcatta aaatcaagat taaatatgtt
     1501 ctggggagta aaagatgtgt ctctccactc cataaagcca agtattgcat ttatttttat
     1561 gcttccactt tgatcaaatc caccaagatt tgcatatgct atttcaggct ctatctctct
     1621 tccatcttct ccataaatct ctatcataaa gaacggagga agctgattgt cgcatatgcg
     1681 tgtagctagg tcttgatact caaggataac tgttatcaca tgcatccctt tctccatatc
     1741 aagaaaatac tctgtgtatt gaggatttgt gtaatggaac cttgtaggtc catctccggc
     1801 aaattcaccg tcaaggtaga atatgtattg tgtgcgtcca gtcatattca gtctgacctt
     1861 ttctttctca gtctcaaact tgacccttat gcagtaatga gcatgtagtt ttttctccca
     1921 ttgtaaaaaa gccatactct tcactcccaa tacttttcta atttacctat ttagtatagc
     1981 ataataatta taaatgtaca tccaaagaag aacattaatt tacaattttt tacttgcaaa
     2041 ctttttaatt tgtatatatt ataaacagac aaacaaagga tttgacattc gtaatttcaa
     2101 tctgcaagtt catgccatat tcgcatttta atcaaagcat acttaccacg tcctaaaaaa
     2161 ctattatagg aggtcggtgg catgcgccaa aagagtattt ttaataacat ctatcacgtg
     2221 ataaacattg gtgtgggtga catgatcatt ttcagaaatg aggaggacaa ggattatttc
     2281 tgccaacttg tgcagaagaa cgtccaaaaa cacagtatta caatttacgc atatgcgata
     2341 atggacaacc atttccattt ttgcttgagt tcacctgact ttaataatat ttcgaacttt
     2401 atgcgggatg tatgtgctcc ttatgccaga tactttaacc gtacggaaat cacaatgaaa
     2461 aacaacaagc ctaccaaaag acacggtgct gtgtttgccg aagcgttcaa agctatccct
     2521 gttaacagtc tgaaccagat ggttactctt gttaattaca tacacaataa tccttgttcg
     2581 gcaaaggaag atccaaaaac atatgtgtac agcagtttta cgaagtatct caatatactt
     2641 tacaagatta agaatgagaa ggctgcggaa aagaaatggt ccatctacat taacacttcc
     2701 tacttcagag aactgtcgta taaagagttt atcaaactta tgacaaggga tgaaaacgac
     2761 cggttctgcg aaggagactc tgaattgaat ggcaaatact atatgtcgga caccatggtc
     2821 ataaaagaag tggaaaatgt ttttaaaatg aagata
//
LOCUS       DAONVY010000305         2845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24054_length_2845_cov_3.546237, whole genome shotgun sequence.
ACCESSION   DAONVY010000305 DAONVY010000000
VERSION     DAONVY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24054_length_2845_cov_3.546237"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLI11_08055"
     CDS             <1..462
                     /locus_tag="PLI11_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52858.1"
                     /translation="GADMGYLLERVSYLKGLADGMNISSDTSEGRLLREIIEVLDDIA
                     MTVEELEDDFATTADDLEDRTEFLESVLFDDDVDDDYFDYDDEDIDTIVCPACKGIIT
                     FSEDQVNEDFTVFTCPACGEEIELELYDDDECCMCDICGDYDDEDDEELDD"
     gene            complement(495..1100)
                     /locus_tag="PLI11_08060"
     CDS             complement(495..1100)
                     /locus_tag="PLI11_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52859.1"
                     /translation="MVPEYIKHAGKVIKEDDTHTEVELVCECGEKCFHVFKNEPTDEE
                     ISKIAQWEKRLKGFRKIEGMSDEEGNVFLVKRNIFGKIVDKVRISDMPEVSKINFLKS
                     ICVSCGREYVVFDNRIHGYDANCESETKDISYNGFVFAKVSDEALRIVVKYENNSESY
                     EEYVESFGYVSREKFSNMFGWIYIYGYNEKSRRLIFEEETS"
     gene            1231..1605
                     /locus_tag="PLI11_08065"
     CDS             1231..1605
                     /locus_tag="PLI11_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52860.1"
                     /translation="MYINKLTEFDYWLDSVLRKKLPSGTVAVNFNLYDNVDDEWSVEL
                     VATDEFDEEDEDWACCEVFTTRGNPFGIKHNGTWEDVQKIFEYNVSNYLKNGKFAHKL
                     RNYQAVGIGFVDRNITILYKKD"
     gene            complement(1638..2711)
                     /locus_tag="PLI11_08070"
     CDS             complement(1638..2711)
                     /locus_tag="PLI11_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPZ52861.1"
                     /translation="MKTRIELFREKLSEKQIESALVTSWQNVFYLSGFTGFGDAFIFI
                     TQESQYILTDSRYLVQAKEECVDFEIKNIPVSDFKKIAGFIKEQNVKTIGFEDYNISY
                     LTYKKVTELCENCEFIAIGGIIEGLRCVKSVEEIELIQRACDIVVETFNDVLGIIKEG
                     VSEKEVAAEIEYGIRKRGAEKTSFDTIVASGFRGALPHGTASDKKIMKGEAITMDFGA
                     FYNGYCSDITRTVFLGEPDEKMRKIYDVVLAANIKAINSFESGMTGRDLDLIAREHIA
                     GEGYGDEFSHGLGHGVGVQVHEEPRINRFGDKELAKGMIFSIEPGIYVEGLGGVRIED
                     LVTVVDGKLKVLTKKAPKEMIIL"
BASE COUNT          820 a          638 c          484 g          903 t
ORIGIN      
        1 ggtgctgata tgggttattt gttggaaaga gtaagctacc tgaaaggtct tgcagatggt
       61 atgaatataa gtagtgatac ctccgaagga aggctactta gggaaatcat agaggtattg
      121 gacgacattg caatgactgt tgaagaactg gaagatgact ttgcaacaac cgctgatgat
      181 ctagaggatc gtacagagtt tttggaaagc gtcctgttcg atgatgacgt agatgatgat
      241 tatttcgatt acgatgatga ggacattgat acaatcgtct gtcctgcctg caaaggtatc
      301 attacattct cagaagatca ggtaaatgaa gacttcacag tattcacctg cccagcatgc
      361 ggcgaagaga tagaacttga gttgtatgac gacgatgagt gctgcatgtg tgatatctgt
      421 ggtgattacg atgacgaaga tgacgaagaa ctggatgatt agttaattga aagggtgcaa
      481 aatgcaccct tttcttatga cgtttcttct tcaaaaatca gtctccttga tttttcgtta
      541 taaccatata tatatatcca tccaaacatg ttggagaact tttccctgct tacatatcca
      601 aagctttcaa catactcctc atagctttca ctattgtttt catattttac aactatccgc
      661 agggcttcat cagaaacttt cgcaaataca aaaccattat atgaaatgtc cttggtttca
      721 ctctcacaat tggcatcata tccatgaatt ctattgtcaa aaacaacata ctccctgccg
      781 caggacacac atatactctt tagaaagtta attttagaca cctcgggcat gtcgctgatt
      841 cttaccttgt caacaatctt cccaaatata tttcgtttta ctaaaaatac attcccttct
      901 tcatccgaca ttccctcaat ttttctaaag ccctttaatc gtttttccca ttgcgcgatt
      961 ttgctgattt cttcatcggt gggctcattc ttgaatacat gaaaacattt ttcgccacac
     1021 tcgcaaacca gctctacttc tgtatgggtg tcatcttctt taataacctt tcctgcatgt
     1081 tttatatact ccggaaccat attccacctc ctgtaaggat aattatacaa cctagtcatt
     1141 atctgacaac aggtatttct ccttgaaata tattgctcct aatggtataa tctgcatttg
     1201 tgagaattat gatatgaaag cgaggagaaa atgtatataa acaaattaac tgaatttgac
     1261 tattggctgg acagcgtcct tcgcaagaaa ttgcccagcg gtactgttgc tgttaatttc
     1321 aatttatatg ataatgtaga cgatgaatgg tccgtggaac tggtggccac agacgagttt
     1381 gatgaagaag acgaagactg ggcctgctgt gaggttttta ctacaagagg caatccgttt
     1441 ggtataaaac ataatgggac atgggaagat gttcaaaaga tttttgaata caacgtatca
     1501 aactacttga aaaatggcaa gtttgcacac aagctaagga attaccaggc tgtaggaatt
     1561 ggattcgttg acagaaatat tacaatactg tacaaaaaag attaagaaaa gctgccatta
     1621 ttggcagctt tggttttcta taatataatc atctctttcg gggctttctt tgtcaggact
     1681 ttaagttttc catccaccac cgttactaaa tcctctatcc ggactcctcc aaggccttcc
     1741 acgtagatac caggctcaat actgaatatc atgcccttgg caagttcctt atccccgaat
     1801 ctgtttattc tcggttcttc atgcacctga acgcccactc cgtgaccaag tccatgggaa
     1861 aattcatccc catatccctc gcctgcaata tgctctcttg caatcagatc caggtctctt
     1921 cctgtcatgc cggattcaaa tgagtttatt gccttaatat tggcagccaa cacaacgtca
     1981 taaatcttcc tcatcttttc atccggctct cccaaaaaga cggttctagt tatatcggag
     2041 cagtacccgt tgtagaatgc gccaaaatcc attgtaatgg cttcaccttt cattattttc
     2101 ttatcagaag cagtaccatg tggaagagct cctctgaagc cggatgccac tattgtgtca
     2161 aacgaggttt tctcagctcc cctctttcta ataccatact caatctccgc agctacttct
     2221 ttttcactga ccccctcttt tataatccca agcacgtcat taaaggtttc aactacaata
     2281 tcacaagctc tctgaatcaa ctcaatctct tccacactct tcacacatct cagtccctca
     2341 attatgcctc caattgctat gaactcgcaa ttttcgcaaa gttctgtcac tttcttgtag
     2401 gtcaaatacg agatgttata atcttcaaat ccaatggttt tgacattctg ctctttaata
     2461 aatccggcta tctttttaaa atcactgacg ggtatgtttt taatctcaaa atctacacat
     2521 tcttcctttg cctgcaccaa gtatctggaa tcggtcaaaa tgtactggga ctcttgcgtg
     2581 ataaatatga atgcatctcc aaatcccgta aaaccagata gataaaacac attctgccac
     2641 gatgtaacaa gggctgattc tatttgcttt tcgcttaact tttctctaaa caactctatt
     2701 cttgttttca tatcaatact ccattttctc ttaatatctt caaaatctta tcaagttcat
     2761 tcttgccaac tttaacatca ttccaaatct caaacgcaat gaccgcctgc cagaacagca
     2821 tggaatagcc attcaatgta ctggc
//
LOCUS       DAONVY010000306         2842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24085_length_2842_cov_3.315034, whole genome shotgun sequence.
ACCESSION   DAONVY010000306 DAONVY010000000
VERSION     DAONVY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24085_length_2842_cov_3.315034"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLI11_08075"
     CDS             complement(<1..447)
                     /locus_tag="PLI11_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52862.1"
                     /translation="MELDIKKRYYKLASLDSCEEPGFCVSENEIRTAYTIGDDYGLHR
                     SRFSFDMVLKDEIEVGLKLFETRMQGIGLWFTLKDGKILIYNQSSGLSVEINHNIELA
                     KKRKIVFLENLSDITLMVDDMVICVVKIDKDNLTVLEKDEKVLEEVK"
     gene            complement(464..2476)
                     /locus_tag="PLI11_08080"
     CDS             complement(464..2476)
                     /locus_tag="PLI11_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPZ52863.1"
                     /translation="MKYTPPFYGSAYYPESWPKEKIKEDIALMKEMGMNVARIGEFAW
                     SNMEPKEGKYDFSFMLHVVSELDKEGIATIMGTPTCTPPAWLTQKHPEVMIVNSHGVR
                     ETHGARRHACPNNPVYREYTRKIVTNMAQAFKDCDSIIGWQIDNELYNNGNDGRGCCC
                     NVCYGKFLEYLKEKYETLENLNRTWGMYVWSLNYTEWEQIPIPRNNVWHHPSYLTEWM
                     LFQARSYKEYCDFQADILHEYLPEDVPVGTDMMPFLGLDYEDMNEKLDVVQFNYYRFT
                     PNLWDIALWADYMYNVLDKPFWITETATCWGGGVACTWHNDENLCIANTMLPVILGSN
                     ANLYWLWRNHFAGQELMHGSVVNSHGRPMHIANEVKHIASSLRKLEDMINNTYPKRSE
                     IAMHISHHAWTLYMFQPMVNGFDYYNAFTGDVFKPLMESQLRSSVISEGMDLSKYKVV
                     ITSFVPHIERKELNKRILEWVKEGGTWIAGPMTDIRTYVANKYMAKPFGMLEDVTGTR
                     LAFTLPGGRKYTAVSDSGEVLEFKENANSFDAYEADESTKVLYKYREGYLKDYAAITE
                     TKVGKGRIILLGSMPAPESFAGFIKAIAQEEGINPFDASGNVVVQIRKNTETDEDEYL
                     ACVEVTNMEGYITAIFDCVDVIDGSTYKKGERIIMAPFSFRVLRKI"
     gene            2578..>2842
                     /locus_tag="PLI11_08085"
     CDS             2578..>2842
                     /locus_tag="PLI11_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPZ52864.1"
                     /translation="MIIDFHVHIFPDKIAKKAIGNLARISGLTPFTDGTIKDTMDKLK
                     ACGVDKAVFLNIATKPSQMTTINDEAARLNRIYENFTSFGSVYP"
BASE COUNT          791 a          670 c          481 g          900 t
ORIGIN      
        1 ttttacttcc tcaagcacct tttcgtcttt ctccaataca gtaagattgt ccttgtctat
       61 cttgacaaca caaattacca tatcatccac catcaatgtt atatcagaca gattctccag
      121 aaatactatt tttctctttt tagccaactc aatgttgtgg ttaatttcca ccgataaacc
      181 agaggattgg ttgtaaatta gtattttgcc atcttttaat gtaaaccaaa gcccaatccc
      241 ctgcattctg gtttcaaaaa gtttcaaccc aacctctatc tcatctttca acaccatgtc
      301 aaaagaaaaa cggcttctat gaagaccgta atcatcccct attgtgtacg cagtccttat
      361 ttcgttctca gaaacacaaa atccaggctc ttcacaacta tccaaacttg ccagtttgta
      421 atatcgcttc ttaatatcca gctccatata caatcatcct ttctcaaatc tttctcaata
      481 ccctgaaaga aaatggcgcc atgattattc tttcgccttt tttgtatgta ctgccgtcaa
      541 ttacatcaac gcagtcaaat attgcagtaa tatagccttc catgtttgta acttccacgc
      601 atgcaagata ttcatcctca tccgtctctg tattcttacg gatttgaaca accacattgc
      661 cggatgcgtc aaacggattg attccttctt cttgcgcaat tgcttttatg aaacctgcaa
      721 aactttccgg agcaggcatg gaacctaata gaattatcct tcccttgcct acctttgttt
      781 cagtaatcgc agcatagtct ttcaagtatc cttccctgta tttatacaat acctttgtac
      841 tttcatctgc ctcgtaggca tcaaaagagt ttgcattctc cttgaactca agaacctctc
      901 cactgtccga tacggcagta tatttcctgc ctcccggaag tgtaaatgca agccttgtac
      961 cagtaacatc ctccagcatt ccaaaaggct ttgccatata cttgtttgcc acataggttc
     1021 taatatcagt catgggacct gcgatccagg taccaccttc cttcacccat tcaagaattc
     1081 ttttgtttag ttccttcctt tcaatatggg gtacaaagga tgtaattaca accttgtact
     1141 ttgacaagtc cataccttcg cttatcacgc ttgaccttaa ttgactttcc atcagtggtt
     1201 taaatacatc tcctgtaaac gcattgtaat aatcaaaacc gtttaccatg ggctggaaca
     1261 tatacaaagt ccaggcatgg tgggatatgt gcattgcaat ctcgcttctt tttgggtatg
     1321 tattatttat catgtcctca agttttctta aagagcttgc aatatgcttt acttcatttg
     1381 ctatgtgcat gggtctgcca tgggaattta caacggagcc gtgcataagt tcctgccctg
     1441 caaaatggtt tctccacagc cagtataggt tggcgtttga gcccaggata acgggaagca
     1501 tggtgtttgc aatgcataaa ttctcatcat tatgccatgt gcatgcaaca cctcctcccc
     1561 agcaggtggc agtctcagta atccagaaag gcttgtcaag tacattgtac atgtaatctg
     1621 cccacagggc gatgtcccaa agattaggag taaatctgta ataattgaac tggacaacat
     1681 caagtttctc attcatatcc tcataatcaa gaccgagaaa cggcatcatg tcggtaccta
     1741 caggcacatc ttccggcaga tattcatgca gtatgtctgc ctggaaatca caatattcct
     1801 tgtaggacct tgcctggaac agcatccatt ccgtaagata ggaaggatga tgccatacat
     1861 tatttctagg aatgggtatc tgctcccatt ctgtataatt cagactccat acatacattc
     1921 cccaagttct gttaaggttc tccaaagttt cgtacttttc cttcagatat tcaagaaact
     1981 tgccataaca cacattacag cagcaacctc tgccatcgtt cccattgtta tacaattcat
     2041 tatctatctg ccatccaatt atgctatcac agtctttgaa ggcctgtgcc atgtttgtga
     2101 ctatctttct tgtatactct ctgtatacag gattattcgg acatgcatgc cttcttgcac
     2161 catgcgtctc ccttacacca tgggagttta caatcataac ctcgggatgc ttttgagtaa
     2221 gccacgcagg aggcgtgcag gtaggcgtgc ccataattgt tgcaattccc tccttgtcaa
     2281 gttccgatac aacatggagc atgaacgaaa aatcatactt accttctttt ggctccatgt
     2341 tgctccaggc aaattcacca attcttgcaa cattcatgcc catttccttc ataagtgcaa
     2401 tatcttcctt gattttctcc tttggccagc tttcaggata atatgcgctg ccgtaaaaag
     2461 ggggagtgta tttcatttta aagacctctt tcacttcatt ttgcgcttat tttatcacct
     2521 ttcggtttgc cattcaataa aaaggggaat ataattaaag gaaaaaggca ggtaaagatg
     2581 attatagatt ttcatgtaca catattccct gacaagatag caaaaaaggc aattggcaat
     2641 ctcgcaagaa taagtggttt gacacctttt actgatggaa ctattaaaga tacaatggat
     2701 aaattgaagg catgcggtgt ggacaaggca gtttttttaa acattgcaac aaagccgagc
     2761 cagatgacca caataaatga cgaggcggca agattgaata gaatatatga aaactttaca
     2821 tcttttggtt cggtttatcc tg
//
LOCUS       DAONVY010000307         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24153_length_2836_cov_3.117224, whole genome shotgun sequence.
ACCESSION   DAONVY010000307 DAONVY010000000
VERSION     DAONVY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24153_length_2836_cov_3.117224"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /gene="polC"
                     /locus_tag="PLI11_08090"
     CDS             <1..533
                     /gene="polC"
                     /locus_tag="PLI11_08090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HPZ52865.1"
                     /translation="AHAVAYVILSLRVAWFKVYYPVEYYSATFTLKVDDFDATNMCHG
                     ADKASSIYLTLKNAENNLSQKEEDQKTVYELVLEMYARGIDFLPIDLYKSDATRFLPE
                     GNAIRPPLCSLSGLGQSAGESIVRARDEGEFLSVEDLKIRSGVNKNVIEVLRQEGCLD
                     NLPETNQLSLFDFSCM"
     gene            596..1990
                     /gene="asnS"
                     /locus_tag="PLI11_08095"
     CDS             596..1990
                     /gene="asnS"
                     /locus_tag="PLI11_08095"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPZ52866.1"
                     /translation="MQKTDIVDIYRDSKAFENKEITVCGWIRTLRDSKTLGFIEINDG
                     SCFKGLQVVFEEGKVENFAEITKLNVGSAIVVKGILILTPQAKQPFEIHASDIYVEGH
                     SSPDYPLQKKRHTLEYLRTIAHLRSRTNTFNAVFRVRSMAAFAIHEFFNKRGFIYAHT
                     PIISTSDCEGAGEMFTVTSLDLENVPKTEDNEVDYSEDFFGQHTALTVSGQLHGECMA
                     LAFGKIYTFGPTFRAENSNTARHAAEFWMIEPEIAFADLDDNMHLAEDMMKYILKYLL
                     ENCKQELEFLNNFYDKNLINRLTLVANSEFGRISYTDAIEILKEHNDEFEYKVYWGCD
                     LQTEHERYLTEKVFKKPVFVTDYPKEIKAFYMRLNDDGKTVAAMDCLVPGIGEIIGGS
                     QREERLDVLEERIKEFDLCREDYEWYVELRKFGGTRHAGFGLGFERLIMYVTGIQNIR
                     DVIPFPRTTGSARF"
     gene            2089..2805
                     /locus_tag="PLI11_08100"
     CDS             2089..2805
                     /locus_tag="PLI11_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPZ52867.1"
                     /translation="MKEDKRFAVLIDADNVSEKYIKLIFDEISNYGVITYKRIYGDWT
                     MPTLSSWKSSLLRYSITPIQQYSYTTGRNSTDSALIIDAMDILYSGNVEGFCLVSSDS
                     DFTRLAARLRESGMYVIGMGEKKTPQPFVVACNKFKYLEALAKDEAMDDPLDTVVTSP
                     EVVREAIRQIVDEISDEDGWTPLSDIGNTLVRRFPDFDVRNYGFTKLTPFIKRLKSFE
                     IKSVKTSNENIKLIYIKNKE"
BASE COUNT          889 a          502 c          684 g          761 t
ORIGIN      
        1 aagctcatgc ggtggcatac gttatacttt cactcagggt tgcctggttc aaggtgtatt
       61 accccgtaga gtattattca gccacattta ctttaaaagt ggatgacttt gatgcaacca
      121 acatgtgtca tggagctgac aaggcatcca gtatctattt gacattgaag aacgcagaga
      181 acaatctgag tcagaaggaa gaagatcaga agacagtata cgaattggtg cttgaaatgt
      241 atgcaagagg catagatttt ctacccattg atttgtataa atccgatgca acaaggttcc
      301 tgcctgaagg aaacgccata aggcctcctt tgtgctcgct ttcaggactt ggtcagtctg
      361 cgggagaaag cattgttcgt gcaagggatg aaggcgaatt cctgtcagtg gaagatttga
      421 aaatcagatc cggagtaaac aagaatgtaa ttgaggttct cagacaggaa ggttgtcttg
      481 acaatctgcc tgaaaccaac cagctttcac tgtttgattt ttcatgtatg tagtgatata
      541 atcagatatt ataacatata aacaagcaac caattttagt taaggaagga tatgtatgca
      601 gaaaacggat attgtggata tatatagaga tagcaaggcc tttgaaaaca aggagattac
      661 tgtttgcgga tggattagaa cgcttaggga ttccaagact cttggattca tagagataaa
      721 tgacggcagc tgcttcaagg ggctgcaagt tgttttcgaa gagggcaaag tcgaaaactt
      781 tgcggaaatt accaagttga atgtgggttc tgcaatagtt gtaaagggca tattgatttt
      841 gacacctcag gcaaagcagc cttttgaaat acatgccagt gatatatatg tggagggaca
      901 ctcctcgcct gactatcctt tgcagaaaaa aaggcataca ctggagtatt tgcgtaccat
      961 tgcccattta aggtccagaa caaacacctt caatgcggtg ttcagagtac gctccatggc
     1021 cgcctttgcc atacatgaat tctttaacaa aagagggttc atatacgctc ataccccaat
     1081 catttcaacc agcgactgtg agggtgcggg agagatgttt actgtgacca gtctggattt
     1141 ggagaatgtg ccaaagaccg aagataacga ggttgactat tcagaagact tctttggcca
     1201 gcatacggct ttgacagtat ccggccagtt gcatggtgag tgcatggctc tggcatttgg
     1261 caagatatat acattcgggc ctactttcag agcagaaaac tccaacacag caaggcatgc
     1321 cgcagagttc tggatgattg agcctgagat tgcctttgcg gacctggatg acaacatgca
     1381 ccttgccgaa gacatgatga aatacatcct taagtatttg cttgaaaact gcaagcagga
     1441 gcttgagttt ctgaacaact tctatgacaa gaaccttata aacagactta ctttggttgc
     1501 aaattcagag tttggaagga tatcatatac cgatgccata gaaatactga aggaacacaa
     1561 tgacgagttt gagtataagg tttattgggg atgcgatctg cagacagagc atgaaagata
     1621 cttgactgaa aaggtattta aaaagcctgt gtttgtaacg gattatccaa aggagataaa
     1681 ggctttttac atgagactca atgatgacgg caagaccgtt gcagcaatgg actgtctggt
     1741 gcccggcata ggtgaaatta tagggggcag ccagcgtgag gaaagactcg atgttctaga
     1801 agaaagaatc aaggagttcg atctttgcag agaagactac gagtggtatg ttgagttgag
     1861 aaaatttggt ggaacacgcc atgctgggtt tggtcttgga tttgagcgcc tgatcatgta
     1921 cgtaacagga attcagaaca taagagacgt tataccattc ccgagaacaa ccggttcggc
     1981 gagattttag tataatagcc tcctgcaaat gcaggagctt tttctgttta aaggagcgtt
     2041 ttttgtgtat aatctaaaca aggagatttt tgtgcaggag tgatggatat gaaagaagac
     2101 aagagattcg cagttctaat tgacgcagac aatgtatcgg agaaatatat taagctcata
     2161 tttgacgaga tatcaaatta tggcgtaata acctacaaga gaatatatgg tgactggacc
     2221 atgcctacct tgagttcatg gaaatcttca ttgcttagat actccataac tccgatacag
     2281 cagtacagct atacaaccgg aagaaactca acagattccg ccctcattat tgatgccatg
     2341 gatatattgt acagtggcaa tgttgagggg ttctgccttg tatccagtga cagcgacttt
     2401 acaagacttg ctgcaaggtt gagggaatct ggcatgtatg ttataggtat gggagagaag
     2461 aaaacacccc agccctttgt tgttgcgtgc aacaagttca agtatctcga agcattggca
     2521 aaggatgagg caatggacga tcctcttgat acagtcgtta ccagcccgga ggttgtaagg
     2581 gaggcaatac gtcaaattgt tgatgaaata tctgacgagg acggatggac tccactaagt
     2641 gatattggaa atactttggt tagacgtttt cctgattttg acgtaagaaa ctacggattt
     2701 acaaagctca ctccctttat taaaaggcta aagagttttg agataaagtc agttaagaca
     2761 agtaatgaga atataaagct tatatatatt aagaacaagg aatagccaaa aaagtcaatt
     2821 aattaagcat gattag
//
LOCUS       DAONVY010000308         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24344_length_2818_cov_4.325009, whole genome shotgun sequence.
ACCESSION   DAONVY010000308 DAONVY010000000
VERSION     DAONVY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24344_length_2818_cov_4.325009"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..239
                     /locus_tag="PLI11_08105"
     tRNA            164..239
                     /locus_tag="PLI11_08105"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Asn,seq:gtt)
     gene            322..1116
                     /locus_tag="PLI11_08110"
     CDS             322..1116
                     /locus_tag="PLI11_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPZ52868.1"
                     /translation="MLGAIIGDIAGSRFGNKRMKSKEFELFSSHCHFTDDTVMTLAVA
                     KALIECNGNYEELDKITEYWMRRLGRQYPRAGYGYRFFTWILSGEHNPYGSFGNGSAM
                     RVSSVGYVTKTIEEVKDLSYKVTYITHNHPEGLKGAEAVAVCVFLAKSGSSIEEIRNY
                     VNENYYTLDFSLDDIRDEYEFDVTCQGSVPQAITAFLESEGFEDAIRNAIALGGDSDT
                     LAAISGSIAEAYYGIPKELADKAVTYMDDYLHGILIEFNDKFGFVE"
     gene            1285..1360
                     /locus_tag="PLI11_08115"
     tRNA            1285..1360
                     /locus_tag="PLI11_08115"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1318..1320,aa:Asn,seq:gtt)
     gene            1501..2556
                     /locus_tag="PLI11_08120"
     CDS             1501..2556
                     /locus_tag="PLI11_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52869.1"
                     /translation="MQLNLPKLFKFDDGSTVENVNDWERRRDEIIDVILKEEYGTIPP
                     RPDNVFVKDMRRVHNDFAGKGEHLIYDLCFMTERGEFSFPVNVVKPKGNKKNPAVVHI
                     AFRPDIPDKYYPAEEVLDLGLTVFTFCYQDVTTDDDFESGLAAMYDRNKWTWGKIGMW
                     AFAAMRVCDLLCTFDYVDSNNIAVMGHSRLGKTALLAGALDERFKYVISNNSGCSGAA
                     ISRGKEGERIKDICNRFGYWFCENYKKYMDNEEKMPFDQHFLLSLICPRYLYVSSAIE
                     DKWADPFNEFITLKEVDKVYCLHGIDGFASEDSFPRSGDVFSEGRVGYHLREGTHYVS
                     RYDWLRFAEFIKKGDRI"
     gene            2553..>2818
                     /locus_tag="PLI11_08125"
     CDS             2553..>2818
                     /locus_tag="PLI11_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52870.1"
                     /translation="MRKRALVLVFLLAYLFAITFGDTYANQENEKQRWYEIKEIDYEF
                     DFEEKKLSFYLTHEALIIEDRANMEIKDGRLYAKGGKSFRFGSSV"
BASE COUNT          871 a          376 c          676 g          895 t
ORIGIN      
        1 gacgatagtg agctggagac ggcttgcgaa aatatgtcgt cgccggattt ttttaaaaat
       61 tctttggata tgtaaagtgt ataaggcgtt gttccaatgg ctttgtgcgt ttttgaattc
      121 aaagaataaa aatattcggt ggtatagacc gccgaaaaca tattcctcaa tagctcagtt
      181 ggtagagcat gcggctgtta accgcagggt cgttggttcg agcccaactt gaggagccat
      241 agagaatcat ccgatatcgt ggatggttct cttttttgtt gattacatgg tataattata
      301 aaaaagtttc aggtgtttta tatgttgggt gcaattattg gggatatagc tggttcaaga
      361 tttgggaata aacgtatgaa aagtaaagag tttgagttgt tttcttcaca ctgccatttt
      421 acagatgata cagtaatgac cttggctgtt gccaaagcat taattgaatg taatggtaat
      481 tatgaagaac ttgacaaaat cacagagtat tggatgagaa gactgggaag acagtatcca
      541 cgtgctggat atggttatag attctttaca tggattcttt ctggggaaca caatccttat
      601 ggaagctttg gtaatggtag tgcaatgagg gttagcagcg tcggttatgt aacaaagaca
      661 attgaagaag tcaaggacct gtcatacaag gtaacgtata taacccacaa tcatcctgaa
      721 ggattgaaag gagcagaggc tgtagcggta tgcgtgtttc ttgccaaaag tggttcatcg
      781 atagaagaga ttcggaatta tgtaaatgaa aactattaca cactagactt ttctctggat
      841 gatataaggg acgaatatga gtttgatgtt acttgtcagg gttcagttcc tcaggcaata
      901 accgcatttt tagagtcaga aggttttgag gatgcaatca ggaatgcaat tgctttagga
      961 ggcgatagtg atactcttgc agcaatttca ggaagcattg ctgaagctta ctatggtatt
     1021 ccaaaggaat tggcagacaa agctgtgaca tatatggatg attatttaca cggtatcctg
     1081 attgaattta atgataagtt tggttttgtt gaataataac atacactagt gggcatgatt
     1141 ctattgtaca gtgttgttag tgtattggtg tcattatata atttctttca aacttggaga
     1201 tttgaaattg aaatctcctg gtttttgttt taatgtattg attttcattt tttgttatgg
     1261 tagaatttta ttaactaggg ataatcctca atagctcagt tggtagagca tgcggctgtt
     1321 aaccgcaggg tcgttggttc gagtccaact tgaggagcca gattcgaggc tgcattgatt
     1381 aatgtatgcc tcttttttct ttgagatatc caattttgtc aggttgtatc ttttttaaaa
     1441 aagctatata atatccatgg aaaaaataaa tttggtgatc tgatgattaa tgagagatta
     1501 atgcaattga atttgcccaa gctgtttaag tttgatgatg gcagtactgt tgaaaatgtg
     1561 aatgactggg agagaagaag agatgagata atagatgtaa ttctgaaaga agagtatgga
     1621 acgatccctc ctcggcctga taatgtattt gtaaaggaca tgaggagagt acataatgat
     1681 tttgctggaa aaggagagca tttgatttac gatttatgtt ttatgacaga aagaggcgag
     1741 ttttcttttc ctgtaaatgt tgtaaagcca aaaggcaata aaaagaatcc agccgttgta
     1801 catattgcat ttaggcctga catacctgat aaatactatc cagcagagga agtgttggat
     1861 ttgggtttaa cagtgtttac tttctgctat caggatgtta caactgatga tgactttgaa
     1921 agtggacttg cagctatgta tgacagaaac aaatggacat ggggtaaaat tggaatgtgg
     1981 gcttttgccg caatgagggt ttgtgatttg ctgtgtacat ttgattatgt ggacagtaac
     2041 aatattgccg ttatgggaca ttcaagacta ggcaaaaccg ctttgcttgc aggagctctg
     2101 gacgagcgtt tcaaatatgt catttcaaat aattcaggct gttccggtgc agccatatcc
     2161 agaggcaaag agggagagag aataaaggat atttgcaacc ggtttggcta ttggttctgt
     2221 gaaaactaca aaaaatacat ggacaatgaa gaaaaaatgc cttttgatca acattttcta
     2281 ttgtccttga tttgtccaag atatttgtat gtttccagtg caatagagga taaatgggcg
     2341 gatcccttta atgaattcat tactcttaaa gaggttgaca aagtatattg tttgcatggg
     2401 atagatggtt ttgcaagtga agacagtttt cccagatctg gtgatgtatt ttctgaaggc
     2461 agagtgggtt atcatttaag agagggaacc cattatgtca gtagatatga ttggttaagg
     2521 tttgcagaat ttataaaaaa aggtgataga atatgagaaa acgagcattg gtactggttt
     2581 tcctgttggc ttatctgttt gcaataactt ttggagatac atatgcaaat caggaaaatg
     2641 aaaagcaaag atggtatgag attaaagaga ttgattatga atttgacttc gaggaaaaga
     2701 aactaagctt ttatcttact catgaagctc tgattattga agatagagca aatatggaga
     2761 taaaagacgg aaggttgtat gccaaaggcg gaaaaagctt caggtttgga agcagtgt
//
LOCUS       DAONVY010000309         2801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24528_length_2801_cov_3.449017, whole genome shotgun sequence.
ACCESSION   DAONVY010000309 DAONVY010000000
VERSION     DAONVY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24528_length_2801_cov_3.449017"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLI11_08130"
     CDS             <1..577
                     /locus_tag="PLI11_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52871.1"
                     /translation="VGGDSITNDLAQVFKISLSEAETIKKDYPLATLEVLSNNIDVAI
                     FSLESGTQEILKVAQIVEVMQARIENIFEIVKDRLEEEDVETQYIDRVILTGDGITKF
                     KGVDKVSESIFETKFAPIDFSRLTGMKSVFTNASGMLMYIAMQLPLGRKESVFERVFL
                     KENVTAVKSKEKSGFAKFMDKLKDWVSSFKE"
     gene            637..1800
                     /gene="ftsZ"
                     /locus_tag="PLI11_08135"
     CDS             637..1800
                     /gene="ftsZ"
                     /locus_tag="PLI11_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPZ52872.1"
                     /translation="MGIEYDSEEGKDAKLMVIGIGGGGNNAVDRMSSSDMTNVDFVCM
                     NTDKHVLLKSKTKIKIQLGEKLTRGLGAGANPEIGEKAANESHDDIVACLKGVDMIFI
                     TAGMGGGTGTGAAPVVASIAKEMGILTVGVVTKPFSFEGRRKMQNALMGIEKLKECVD
                     SLVVIPNDNILKVVDEKVTIDNAFKLVDDILYQSVRGICDIITKVGYVNVDFADVKAT
                     MSNRGIVHMGVGRASGKNRSEEALLRAIQNPLLETSIDGAKAVLINFYGDKLNMQEMA
                     NISNVVYEKVDLDAQIIIGTYDAGEEMNDEMQVTVIAAGFDEIEDTMPEEILPPQQAK
                     TPSYSSFGSADSGLAGSQAKESFASNPSQQRQTPRRSLDDDYEVPPFLRKSRR"
     gene            1995..>2801
                     /locus_tag="PLI11_08140"
     CDS             1995..>2801
                     /locus_tag="PLI11_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HPZ52873.1"
                     /translation="MEVYLDILIIENLIMNYLILYATSLFLKSKHNIPRYLVGASIGV
                     LYTVLSLVFMSSAKTSLVVLGKASLSMLIVAIAFNPKRLGEFIKTLITFYVVTFIFAG
                     SAFALIMLGGMGGVTKEGFMYLNWDFPVNYIVPTAAIGMFLFGRFIRYIRERSRASDC
                     FVSLYIAIDGNGVWVPALVDTGNELRDPISGYPVVIVEAKAVSSLIPEKIRELLSKQG
                     LGLGHVDSSLLESGWISRFRIVPYRSIGCENGMLPGFKPDYIEIDDLGQEK"
BASE COUNT          882 a          485 c          682 g          752 t
ORIGIN      
        1 tgttggtgga gacagcatca cgaatgattt ggcgcaggtt ttcaaaatat ctctaagtga
       61 agccgaaaca ataaaaaaag attaccctct tgcaactttg gaagttttgt ctaataatat
      121 agatgtcgca atattttcac tggaatccgg cacccaggaa atattgaaag ttgcacaaat
      181 tgtggaagta atgcaggcca gaattgagaa tatttttgaa attgtcaagg acaggcttga
      241 ggaagaggat gtggaaacac aatatattga cagggtaata ctgacaggag acggcataac
      301 aaagtttaaa ggcgtcgaca aagtgagtga aagcatattt gaaacaaagt ttgcacctat
      361 tgatttcagt aggttgacag gcatgaagag tgttttcaca aatgcaagcg gaatgctaat
      421 gtacattgca atgcagcttc ctttgggaag aaaggaatcg gtctttgaga gagtatttct
      481 caaggaaaat gtaaccgctg taaagagcaa ggaaaaaagc ggctttgcga agtttatgga
      541 caagttaaaa gactgggttt ccagtttcaa agaatgataa taatatgata taatatctct
      601 gttacctaaa cagagataaa taagggggta aacatcttgg gtatagaata cgatagcgaa
      661 gaaggtaagg acgcaaaatt aatggttatt ggaataggtg ggggaggaaa caacgccgtt
      721 gacaggatgt ccagttcgga tatgaccaat gtcgattttg tttgcatgaa tacggacaag
      781 catgtgctcc tgaaatccaa aacgaaaata aaaatccagc ttggtgaaaa acttacaaga
      841 ggtcttggtg ccggagcaaa tcctgagata ggagaaaagg cggcaaatga aagccatgat
      901 gacattgttg catgtttgaa aggcgtggac atgatattca ttacagcggg aatgggcgga
      961 ggtaccggta ccggtgctgc tccggttgta gcgtccattg cgaaggaaat gggaattctg
     1021 acagttggtg ttgtgacaaa accattttct tttgaaggtc gtagaaaaat gcaaaatgcc
     1081 ctaatgggta ttgaaaagct taaggagtgc gttgactcac tggttgtgat tcctaatgac
     1141 aacattctga aggttgtgga tgagaaagtc actatagaca atgccttcaa actggttgac
     1201 gacatccttt accagtctgt aaggggaata tgcgacataa tcacaaaagt tggttatgta
     1261 aacgtggact ttgcagacgt aaaggcaacc atgtcaaacc gtggtattgt ccatatgggt
     1321 gtgggacgcg cttccggaaa gaacagaagc gaagaagctt tgcttagagc aatacagaat
     1381 cctctgcttg aaacaagcat agatggagca aaagctgtat taattaactt ctacggagac
     1441 aaactgaata tgcaggagat ggcgaatatc tcaaatgttg tatatgaaaa ggttgatctt
     1501 gacgcacaga taatcatagg aacatatgac gcaggcgaag aaatgaatga cgagatgcag
     1561 gttactgtta ttgcagcagg tttcgacgag attgaagata ccatgcctga ggagattctg
     1621 ccaccacagc aagcaaaaac tccaagctat tcaagctttg gcagcgctga tagcggactt
     1681 gcaggctctc aagcaaaaga gagctttgca tccaatccat cgcagcaaag acagactcca
     1741 agaagatcct tggatgatga ctacgaggtt cctccattct tgcgcaagtc aagaagataa
     1801 ttattgtgtc cgttagaaat atttcccaaa gagggttgat taaagccttc ttttaatacc
     1861 caattggcat aaatgcagtg tgatatttat tcacactgtt ttttttatat gcgaaaaaac
     1921 tcgtctattt tcgacaatta aaacaggtat aatgtatcca caatcagaat aatatatgac
     1981 aggaggtgtt ttggatggaa gtgtaccttg acatcctcat tattgagaac ctcataatga
     2041 actaccttat actatacgca acttcgcttt ttttgaaatc aaaacacaac attccaaggt
     2101 atcttgtagg ggcctcaata ggtgtcttgt atacagtcct gagccttgtg tttatgagct
     2161 ctgcaaaaac ctcgctggtt gtccttggca aggcatcctt gtccatgctc attgttgcca
     2221 tagctttcaa tccaaaacgc cttggggaat tcataaaaac gctgataaca ttctatgtcg
     2281 tcactttcat attcgcaggc agcgcctttg cgcttatcat gcttggaggc atgggaggtg
     2341 ttacaaagga aggattcatg tatctgaatt gggattttcc tgttaactac atagtcccca
     2401 ctgcagccat tggcatgttt ttgtttggga gattcataag atacataagg gaaagatcaa
     2461 gagccagcga ttgttttgta agtttgtaca ttgccattga cggaaacgga gtttgggttc
     2521 ctgcccttgt ggataccgga aatgagctaa gagatcccat atcaggatat cccgtggtaa
     2581 tagtggaggc taaagcggtt tcgtcattga ttcctgagaa aataagagag ttattgtcaa
     2641 aacagggatt gggtcttggg catgtagatt catctctgtt ggaatcgggc tggataagca
     2701 ggttcaggat tgtgccttac cgctccatag ggtgcgaaaa tggaatgctg cctgggttca
     2761 agcccgacta tatcgagatt gacgacttgg ggcaggagaa a
//
LOCUS       DAONVY010000310         2787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24682_length_2787_cov_3.006955, whole genome shotgun sequence.
ACCESSION   DAONVY010000310 DAONVY010000000
VERSION     DAONVY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24682_length_2787_cov_3.006955"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..995)
                     /locus_tag="PLI11_08145"
     CDS             complement(93..995)
                     /locus_tag="PLI11_08145"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPZ52874.1"
                     /translation="MKHLLGLKDTSKEEIENILETARGMQVILDSNIKKAPHLNGKSI
                     VTLFYENSTRTRLSFELASKYLSSASANIAASASSVAKGETLLDTGRTIQRMGTDVII
                     IRHPMAGAPHLLAKHLKCSIVNAGDGMNEHPTQALLDMYTMLRHKGTLHGLKVVVTGD
                     VLHSRVARSNIWGLTKMGAEVVVNGPATMMPVAIEEIGAKVEPNIKNAVKDADVIMGL
                     RIQLERQKNGLFPTIREYSKFFSVNEEVLSYAAKDCLIMHPGPVNRGVEMTSMLIDGD
                     SSVIEEQVTSGVAVRMAILYILTR"
     gene            complement(998..1531)
                     /gene="pyrR"
                     /locus_tag="PLI11_08150"
     CDS             complement(998..1531)
                     /gene="pyrR"
                     /locus_tag="PLI11_08150"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPZ52875.1"
                     /translation="MEKALIMDKDAMNRALTRIAHEIVEKNKGVEDVALIGIQRRGVP
                     LAKRIAQKIEEIENVKVPLGVLDITFYRDDLSLLAAHPVINGTDIGFQIEGKKIVLVD
                     DVIYTGRTVRAAIECLFDLGRPQMVQLAVLIDRGHRELPFRPDYVGKNIPTSKSEVVS
                     VQVVEIDSIDKVTINEG"
     gene            complement(1772..>2787)
                     /locus_tag="PLI11_08155"
     CDS             complement(1772..>2787)
                     /locus_tag="PLI11_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52876.1"
                     /translation="YGNMPIDKIDWELVKCIAIIELYLNERWIEAVVPGKYPYEILLH
                     QTLSHVASVHETRVAKLAEYILTLSPFRNISQEDYRLLLLKMLEDGLLERTEEGFLHV
                     GPLGEAIVSNYDFFSVFETSIEYSVIYENREIGQVMVPYEEEETFSLAGRAWQVVSID
                     KNSHVIYVKPYDEHAESKYKSYIRLIVPTKLVEKVFEVLSSDKTYGYLGKRALDRLVS
                     SRNMFAAISIKDDVVQTGDKCLFVPKLGTKEIMYFSHALKMHGYRNEICYASFVPIYI
                     SIPDSYAKDVRAVIREIVKNKPDKYDFHVNVYNLGGKLRKYLPDVLTRKEFITDYIDD
                     LRC"
BASE COUNT          754 a          698 c          446 g          889 t
ORIGIN      
        1 cttgtcgctg ccgtcatcaa ggtgtatgtc cattaccttg tcttcattta ctgatacgta
       61 tcgtatattc ttaaatacca tgtcacacct ctttatctag taaggatata caaaattgcc
      121 attctaacgg caactccgct agttacctgc tcctcgatta ccgatgaatc tccgtcaatc
      181 agcatggaag tcatttcaac tcccctgttg acaggaccag gatgcattat caagcagtcc
      241 tttgctgcat atgacaaaac ttcctcatta acactgaaga atttgctgta ctcccggatg
      301 gtcggaaaca atccgttttt ctgtctctcc agctgaatcc taagacccat tattacatcc
      361 gcgtccttaa ccgcattttt aatattcggt tccacttttg cacctatttc ttcaatagct
      421 acaggcatca ttgtggcagg tccgttcact accacttcag cacccatctt tgtcaatccc
      481 cagatgttgc tccttgcaac tcttgaatgc aaaacgtcac ctgtaacaac caccttcaaa
      541 ccgtgcaatg tccctttgtg cctgagcatt gtgtacatgt caagcaatgc ctgggtggga
      601 tgttcattca ttccatctcc ggcattcact atgctgcatt ttaaatgttt tgccaacaag
      661 tggggagctc ctgccatcgg atgcctgata attattacgt ccgttcccat tcgttgaatg
      721 gtcctgcctg tatcaaggag agtttctcct tttgcaacac tgcttgccga tgctgcaatg
      781 tttgcagatg cgctggatag atatttcgat gccaattcaa aagaaagcct ggttcttgta
      841 ctgttttcgt agaaaagagt aacaatggat tttccattca aatgaggtgc cttcttgatg
      901 ttcgaatcaa gaattacctg catgcctctt gccgtttcca aaatgttttc aatctcttct
      961 ttggatgtat ctttcaaacc taacaaatgc ttcataacta accctcgttt atcgttactt
     1021 tgtcaatact gtcaatttcc accacctgca ctgagaccac ctccgacttg ctggtaggaa
     1081 tgttctttcc aacataatca ggtctgaaag gaagttccct gtgacctctg tctatcaaga
     1141 ccgcaagctg taccatctga ggtcttccca aatcgaacag gcactcaatt gccgcccgta
     1201 ccgtcctgcc tgtgtagatt acatcatcca ccaggacgat tttctttcct tcaatctgaa
     1261 acccaatatc agttccattg attacaggat gtgcagccaa caaactaaga tcatccctgt
     1321 agaatgtaat atcaagaacg ccaagcggca cttttacatt ctcaatctcc tcaatctttt
     1381 gagctattct ttttgccaaa ggaacgcctc ttctctgtat tccaatcaga gcaacatcct
     1441 caacgccctt gttcttctca acaatttcat gggcaattct ggtaagagct ctgttcatgg
     1501 cgtccttgtc cattatcaat gctttttcca taagtcatcc tttccgcaaa tttgcaaaaa
     1561 aaattgttcc gtacctggag agagggcacg aaacaatcat aataatccca taattatgtc
     1621 gttaccttac ccgtctctct ggaccagttt aaaggtacac ctatattata tccaattcct
     1681 gatattaatc aatatgtttt ttcaaacact acaaccttaa cttcttcatc caacttccat
     1741 acccattgtt ggaagcaaca cttatccttc attaacatcg caagtcgtca atataatctg
     1801 taatgaattc cttcctcgtc aagacatcgg gcaaatactt tcttagcttt ccacccaagt
     1861 tataaacatt tacatgaaag tcatacttgt ctggtttatt tttcacaatc tcccttataa
     1921 cagctcttac atcctttgca taactgtctg gtattgaaat atatattggt acaaagcttg
     1981 cataacaaat ctcatttcga tacccatgca tctttaaagc atgggaaaaa tacataattt
     2041 cttttgtacc aagctttggc acaaacaaac acttgtctcc tgtttgaacc acatcgtctt
     2101 taatacttat agctgcaaac atattccgtg atgaaaccag tctgtccaag gcccttttgc
     2161 cgagatatcc ataagtttta tccgatgata atacctcaaa caccttttct accagcttcg
     2221 ttgggactat caacctaatg tatgatttgt atttgctctc cgcatgctca tcataaggtt
     2281 ttacataaat aacatgcgaa ttcttgtcaa ttgaaacaac ctgccaagct ctccctgcaa
     2341 gggagaaggt ttcttcctct tcgtaaggaa ccataacctg tccaatctcc ctgttctcat
     2401 aaatcacaga atactctata gaagtttcaa atacggaaaa gaaatcataa ttggatacaa
     2461 tagcttctcc aaggggacct acatgcagga atccctcttc cgttctttcc aaaagcccgt
     2521 cttcgagcat ttttaaaagc agcagtctgt aatcttcctg ggagatgttt ctgaagggac
     2581 tcagtgtcag aatatattct gcaagttttg ctacccttgt ctcatgtacg gaagcaacat
     2641 ggctaagcgt ctgatgaaga aggatttcgt atggatattt tcctgggact acagcctcta
     2701 tccacctttc gtttaaatat agctcaataa ttgctatgca ttttacaagc tcccaatcta
     2761 tcttgtctat aggcatgttg ccatagc
//
LOCUS       DAONVY010000311         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24709_length_2785_cov_2.590110, whole genome shotgun sequence.
ACCESSION   DAONVY010000311 DAONVY010000000
VERSION     DAONVY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24709_length_2785_cov_2.590110"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..1365
                     /gene="radA"
                     /locus_tag="PLI11_08160"
     CDS             10..1365
                     /gene="radA"
                     /locus_tag="PLI11_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPZ52877.1"
                     /translation="MAKSKTKFYCTECGYESAGWLGKCPGCTQWNTFVEEKITKEVSG
                     AGNTGIRNTPKAVSINEVSIEDEKRLSTSIDELDRVIGGGVVKGSMILIAGDPGVGKS
                     TLMLMLSGNIAKSNRVLYVSGEESVRQIKMRAKRLNVDAKELYLVSETNIDSVTRLIE
                     EFQPGYLVVDSIQTLYDDAIQSAPGSVSQVRETTNTLMQIAKTQGISVFIVGHVTKGG
                     AIAGPMVLEHMVDTVLYFEGERHQTYRVLRSVKNRFGSTNEIGVFEMKENGLVEVQNP
                     SLALLSGRPVDCPGTAVVCTMEGTRPMLIEVQALVTRSGFMNARRMATGFDVNRLSLL
                     IAVMEKRLGLKLSNFDVYVNVIGGIRITEPAVDMPVVAAILSSYRDLSLDPEMVFCGE
                     IGLTGEIRTVSHVDKRVSEAIRLGFTKCIIPKGNKLKSQDSDKIVSISSIQELNELLL
                     K"
     gene            1383..1937
                     /locus_tag="PLI11_08165"
     CDS             1383..1937
                     /locus_tag="PLI11_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPZ52878.1"
                     /translation="MKTLMLISNGFEEIEAITVIDILRRCDIEVDVASITGSREITGA
                     HDIKMVTDTLIEDLNAEGYDGLLLPGGLPNAHNLRDDERVINLVKEYDKNGKVLAAIC
                     AAPCILERAGVLKNKNVTAYPNCIDESAVNYKKVKAVRDGNVVTGCGVGGAIDFALLI
                     AKSLGKEEEAEKVRKGILYDWPLE"
     gene            complement(1916..2737)
                     /locus_tag="PLI11_08170"
     CDS             complement(1916..2737)
                     /locus_tag="PLI11_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52879.1"
                     /translation="MPERKRFSYLFSEAVNIFRRNISIFLLLTLFVFLPLSLANVYIA
                     DRIIDTELLNDYLSNFESYLNTTDYEKIANISALTKQFTTYILVSFILSLFSLVGDIV
                     VIKTTYERQNGIHRSFFEVFEDSVKQFPKVLGIFLITRFFISLGIFLIIPSLYMIFLF
                     GFVIQVVVIYNIGGFKALRHAVRVSRTDLLRVVGYKLFPVLLLVPLTFVSSILFEFIS
                     NKYVIDVINVIKLSLDSLIMCIGTILVTLLFFDMQKLVGEPVDIMSAQNYSSGQS"
BASE COUNT          882 a          475 c          660 g          768 t
ORIGIN      
        1 agaatagcca tggcgaagag caagacaaaa ttctattgta ccgaatgcgg atacgagtcc
       61 gcaggctggc ttggcaagtg tccaggctgt acccaatgga atacatttgt ggaagagaag
      121 ataaccaagg aggtttctgg tgccggcaat acaggcatca gaaacactcc taaagccgta
      181 tccatcaatg aggtatccat cgaggatgag aaacggttaa gcacatccat tgatgagctg
      241 gacagagtta ttggtggggg agttgtcaag ggttcgatga ttttgattgc gggagacccc
      301 ggtgtcggaa agtcaactct tatgctcatg ctatccggaa atatcgcaaa aagcaacagg
      361 gttttgtatg tatctggaga agaatctgta cggcagatca agatgcgtgc caaaaggctt
      421 aatgtggatg caaaagagtt gtatttggtt tcggagacaa atatagactc cgttacaagg
      481 ctgattgaag agttccagcc gggttatctt gttgtagact ccatacagac attgtatgat
      541 gatgcaatac aatcagcacc aggtagtgta agccaggtta gagagactac aaatactctt
      601 atgcagattg caaagacgca gggcataagt gtgttcatag tcggtcatgt aaccaaggga
      661 ggagcaattg ctggtcctat ggtgcttgag cacatggtag ataccgtact gtactttgaa
      721 ggtgaaaggc accaaactta cagagtttta agaagtgtta aaaacaggtt tggttccaca
      781 aatgaaatag gcgtatttga aatgaaggaa aatggccttg tcgaggttca gaatccttca
      841 ctggcgcttc tctctggacg tcctgtggat tgtccaggca cagccgttgt atgtaccatg
      901 gaaggtacaa ggcccatgct tattgaggta caggctctgg tgaccagatc cggtttcatg
      961 aatgccagaa ggatggcaac gggttttgat gtgaacagac tgtcattgct cattgctgta
     1021 atggaaaaaa ggctgggctt gaaactgtcc aatttcgacg tgtatgtgaa cgttataggg
     1081 ggtattagga ttaccgagcc cgctgtagac atgcccgttg tggctgctat cttgtcatcc
     1141 tacagggatt tgagcctgga tcctgagatg gtattctgtg gtgagattgg tttgacgggg
     1201 gagataagga ctgtaagcca tgtggacaag cgggtatctg aagccataag gcttggtttt
     1261 acgaagtgca ttattcctaa aggaaacaag ctaaagtcac aagatagtga taaaatagtt
     1321 tcaataagct ctattcagga gctgaatgaa ttgttgttaa aataaaggga ggatgattgt
     1381 ttatgaagac attgatgcta atttcaaacg gttttgagga gattgaggca ataacggtca
     1441 ttgatatctt gagaagatgt gatattgagg ttgatgttgc aagcataaca ggaagcagag
     1501 aaataaccgg agctcatgat atcaaaatgg ttactgatac attgattgaa gatttgaatg
     1561 cagagggtta tgatggtttg cttctccctg gcggactacc aaacgcacac aatttaaggg
     1621 atgatgaaag ggttattaat cttgtaaaag agtatgacaa aaatggcaag gttcttgcag
     1681 caatatgtgc cgcaccttgc atactcgaaa gagcgggtgt attaaaaaac aagaatgtta
     1741 ctgcatatcc taattgcatt gatgaaagcg ctgtgaatta caaaaaagta aaagcagtaa
     1801 gggatggcaa tgtcgttacc ggctgtggtg taggcggtgc tattgatttt gcattactta
     1861 ttgccaaatc ccttggaaag gaagaagagg ccgaaaaggt acgcaaaggt atcctttatg
     1921 attggccgct tgagtaattc tgagcagaca tgatatctac aggttctccg acaagctttt
     1981 gcatgtcgaa gaacaagagt gtaacaagaa ttgttcctat gcacataatg aggctgtcta
     2041 aagacagctt tattacattt ataacatcaa taacgtactt gtttgatata aattcaaaca
     2101 agattgatga aacaaaagtg agcggaacaa gtagtaacac tggaaacaat ttatatccca
     2161 cgactcttag aagatcggtt ctcgatactc taaccgcatg ccttaatgcc ttgaatcctc
     2221 caatgttgta gattacaacc acttggatta caaatccaaa cagaaagatc atgtaaagac
     2281 ttgggattat cagaaagatt cccagagaga taaaaaatct agtaatgaga aatattccga
     2341 gaactttggg aaactgtttt acagaatcct caaagacctc gaaaaaggac ctatgtattc
     2401 cattctgcct ttcatatgta gttttaatta ccactatatc tcctacaagg gagaacaacg
     2461 acagaataaa ggatacaaga atatatgttg tgaactgttt tgtcagagca gatatgtttg
     2521 caattttctc ataatccgta gtattgagat aggactcaaa attggaaaga taatcattta
     2581 acagttctgt atcgattatc ctgtctgcaa tgtaaacgtt tgccaatgaa agcggcaaaa
     2641 atacaaacag tgtcaataat aggaaaattg aaatattcct tctgaatatg ttcacagctt
     2701 cgctgaataa ataactaaat ctttttcttt ctggcatttc tctttctgta ctccttgtat
     2761 actaccttgt catatttcaa gaact
//
LOCUS       DAONVY010000312         2780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24765_length_2780_cov_3.370642, whole genome shotgun sequence.
ACCESSION   DAONVY010000312 DAONVY010000000
VERSION     DAONVY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24765_length_2780_cov_3.370642"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLI11_08175"
     CDS             complement(<1..811)
                     /locus_tag="PLI11_08175"
                     /inference="COORDINATES: protein motif:HMM:NF037180.2"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPZ52880.1"
                     /translation="MNSTVRRLFVEKKKGLDVEAQLLLKDLKSTLGIKNLDNVRILNR
                     YDVEYLNNSVFDEAKITIFSQRNADIVYEEVFDVGENDYVFTVELLPGQYDQKADSCE
                     QSLRILAPRDNPIVKTAIVYVLTGNLEKTDIDKIKKYIINPLDSREDLLPKPVSLDTK
                     TKSAASIKNLEGFITMSDDEVEKLCNELGLAMSLEDLIFVRNYFRETERRDPTITEIR
                     VLDTYWSDHCRHTTFHTKLVEVDLPENSVIKKTFEDYLMARKDVYGDREDKK"
     gene            complement(919..>2780)
                     /locus_tag="PLI11_08180"
     CDS             complement(919..>2780)
                     /locus_tag="PLI11_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52881.1"
                     /translation="KNNLKAFKKYLEENANNLDFLSSIQYMYDLNLNIYSKDVEGKIF
                     KSDIQELMNRFMGGGSSQNRYSSPFSSSFNVWQEIMPGKNGETIADTVYEQYDVIYGK
                     MLENHDEIVLIFDEKNEISDIVLFSLGFITEEQMMEIMMAGAEGRTVELDLKDSWSYE
                     EICEMTFKVVLNADKYQKQDNGRYVDLSETEAGLAFLYDNASVDLKVVGILRPKEEAV
                     ATSISGGIGYTSKLTEFVINETSSREIVKEQLANPDIDVITGLPFKNEEAEELTEEEK
                     IIKVNEYFAALDNMEKAALYIKIMSVPSSEYLESAVTQTLRSMSEEEIKQMLVKAYAE
                     QMGLDEEQIAAYVENMDKETRTEYASQILAASISMEYAKQVQQELGEYPSEQLVYLFD
                     NAQFTKEQYLYVFENLMPATESESTLEDNLKLLGYVDLEDPFIINLYATTFENKQLIA
                     DMIKKYNRGVSENDKISYVDYIELFMSSITTIISAISYVLMAFVAISLIVSSIMIGII
                     TYISVLERTKEIGILRAIGASKKDISRVFNAETLIVGFVAGALGILITLFLIVIVNII
                     LLNLTGIEALRATLPIGGAIVLVLISMFLTLIAGLIPSKIASKKDPVIALRTE"
BASE COUNT          755 a          599 c          465 g          961 t
ORIGIN      
        1 cttttttgtc ttccctgtca ccatatacat cttttcttgc cataaggtaa tcttcgaaag
       61 tcttcttgat tacgctgttt tcaggcaagt ccacctcaac tagttttgta tggaatgtag
      121 tatgcctgca gtggtcagac caatatgtgt caagtaccct gatttcagtt attgtaggat
      181 ctcttcgctc cgtttctctg aaatagttgc gtacaaaaat caggtcttcc agagacattg
      241 ccagtccaag ttcgttgcaa agcttttcaa cctcgtcatc ggacatggtt atgaatcctt
      301 caaggttctt aatgcttgca gcagattttg ttttagtatc aagcgaaact ggttttggta
      361 ataaatcctc tctggaatcc agagggttaa taatatattt tttaatttta tctatgtcgg
      421 ttttttcaag atttccggtt agaacgtata ctattgcagt tttaacgata gggttgtcac
      481 gtggtgcaag aattctcaaa ctttgttcgc aggaatcagc cttttggtca tattgtccgg
      541 gaagaagttc aacggtaaac acatagtcat tttcaccgac atcaaacact tcttcataaa
      601 caatatctgc atttcgttgt gagaatattg taatttttgc ttcatcaaaa acagaattgt
      661 tcaaatattc aacatcatac ctgttcaata ttcgtacatt atcgagattt ttaataccaa
      721 gagttgattt aaggtcttta agtagtaatt gtgcttccac atccaaacct tttttctttt
      781 cgacaaacaa tcttctgaca gttgagttca ttgtttaacc cccaaaaatg atataacgta
      841 ctcaaatatt atacattaat ttactgtgaa atacaagaaa ttaaaaaaga actgccgtgt
      901 gtaggcagcc cttaaagatt attcggttct caatgctata accggatctt tcttcgaagc
      961 aatcttcgaa ggtatgagtc ctgcaatcaa tgtcaggaac atgctgataa gaacaagtac
     1021 tatcgctcca cctatcggca gtgtcgctct tagagcctct attcctgtta gatttaagag
     1081 tattatgttt actattacaa tcaggaacag agtaatcaga atacccagtg caccggccac
     1141 aaatccgact atcaaagtct ctgcattaaa tactctggat atgtctttct tggatgctcc
     1201 aattgcacgc agtataccaa tttccttcgt tctttccaga accgaaatgt acgtaattat
     1261 gcctatcatg attgacgaaa caatcaggga tattgcaaca aaagccataa gtacatagga
     1321 tatcgcgcta attattgtgg ttatagatga cataaacagc tctatataat ccacatagga
     1381 tattttatca ttctcggaca cccctctgtt gtacttcttt atcatgtctg caatcaactg
     1441 tttgttttca aatgttgttg cataaagatt tatgatgaag ggatcttcca aatccacata
     1501 tcccagaagc ttcaagttgt cttcaagcgt gctctcagat tccgttgccg gcatcaggtt
     1561 ttcaaataca tacaggtact gttcttttgt aaactgtgca ttatcaaaca agtataccag
     1621 ctgttccgat ggatattcgc caagttcctg ctgtacttgc ttggcatatt ccatggaaat
     1681 ggaagctgca agtatctgtg aagcatactc ggttcgtgtt tctttgtcca tgttttcaac
     1741 atatgctgca atctgttctt cgtcaagacc catttgctca gcatatgcct ttacaagcat
     1801 ttgctttatc tcttcctcgc tcattgacct tagagtttgc gttacagcac tttcaagata
     1861 ctcactgctt ggtaccgaca tgattttaat atacaatgca gctttctcca tattatcaag
     1921 agcggcgaaa tactcattca ccttgatgat tttttcttct tccgtaagtt cctccgcctc
     1981 ttcgttcttg aatggaagac ctgtaattac atctatatcc ggatttgcaa gctgttcctt
     2041 gactatctcc ctgcttgaag tttcattgat aacaaactcc gttagttttg aagtataccc
     2101 tattcctccg ctgattgatg tggctaccgc ttcttccttc ggacgaagga ttcccactac
     2161 tttcaagtca acagaagcat tgtcataaag gaaagcaagc cccgcctctg tctcactcaa
     2221 gtccacatac ctgccattat cctgtttttg atatttgtct gcattcaata ccaccttgaa
     2281 ggtcatttcg cagatttctt catatgacca ggagtccttg agatctagct caactgttct
     2341 tccctctgct cctgccatca ttatttccat catctgttct tcggttatga atcccagtga
     2401 gaacaatact atgtcgctta tttcattttt ctcgtcaaag ataagtacta tttcatcatg
     2461 gttttctagc atcttgccat aaataacatc atactgttcg tagacggtat ctgctatggt
     2521 ttcgccattc tttccaggca ttatttcctg ccatacatta aagcttgagg aaaatggact
     2581 ggagtacctg ttttgagaag aacctccccc catgaatctg ttcatcaatt cttgtatgtc
     2641 ggatttaaat attttgcctt caacatcttt tgagtaaatg ttcaggttca aatcatacat
     2701 gtattgtata gaagacagaa aatcaagatt atttgcattt tcttcaagat acttcttgaa
     2761 ggccttaaga ttgtttttcc
//
LOCUS       DAONVY010000313         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24835_length_2774_cov_3.839279, whole genome shotgun sequence.
ACCESSION   DAONVY010000313 DAONVY010000000
VERSION     DAONVY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24835_length_2774_cov_3.839279"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..580
                     /locus_tag="PLI11_08185"
     CDS             191..580
                     /locus_tag="PLI11_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52882.1"
                     /translation="MNFFKRFMHGRYGMDQLSFTLLIIAFIILIISRLLLPILGYFAL
                     AIVVYAYYRFFSKDIKKRQGENMKFLSVAKPMQMKIKNKRLLIFGTKTHKYYKCPKCK
                     QMLRVPKNKGLIQITCSRCKKEFIKKT"
     gene            658..744
                     /locus_tag="PLI11_08190"
     tRNA            658..744
                     /locus_tag="PLI11_08190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Leu,seq:taa)
     gene            809..1675
                     /locus_tag="PLI11_08195"
     CDS             809..1675
                     /locus_tag="PLI11_08195"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HPZ52883.1"
                     /translation="MNRQKRIVAIHDISCAGRCSLTVALPIISSVGIETNIIPTAVLS
                     TQTGDLEGYTFKDLTDQIMPIANHWKSLDRTFEAIYTGYLGSFEQIDLVKQIFLMFGN
                     EVCLKFVDPVMADHGVLYDGFDFDFVKGMKDLCGQADIITPNITEAVFMLGEEYIEGP
                     YTDEYINSLLHKLGSICKKVIVTGIDFTDNCYGAASYNVEEGVFQYAKSEKVHGKFHG
                     TGDVFASCLIAALLSDIPMYDSMKMAVDFTVESIKRTKESKSDVRFGINFEAGIFDFV
                     KRIRNFNPGNPF"
     gene            complement(1653..2618)
                     /locus_tag="PLI11_08200"
     CDS             complement(1653..2618)
                     /locus_tag="PLI11_08200"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPZ52884.1"
                     /translation="MGNTDLDGGDESAYRYSYRLKFDKFVKGQTIDGLDELVLSNVSR
                     DPSYMREFLLCEAFEKVELVAPLATYATVTINDNLVGFYVAIESVDDSFLKRNFGNNK
                     GNLYKSNEQATLVDHNSLSLMEQKNGSDETKEDLQLLIDTLNSMPLGEKGDIENIMHV
                     DSVLKYIAVNTVMGNYDSYIGASATNYYLYNVNNKLYMVPWDFNLSFGAQKKDNGVSV
                     ATPVDSPVYRADITERPLVYKILSVEEYYNKYLEYVDICKNFLQDFENRVNEIDSIIS
                     DYIKNDPSSFYSYENYLANIGKSEENTAGIIAITEYASIRRDFLG"
BASE COUNT          832 a          506 c          549 g          887 t
ORIGIN      
        1 cgttccactc cgttcctaaa ggttcttttt gactgataga actcaaaatc cagtggtggc
       61 gacaccgtac cggttcgact ccggtcacca gcaccataaa accgaacttt ttcagtagga
      121 aagagttcgg ttttatttat gtaatttgtg gtataatagt ataaacagag cataatagaa
      181 ggatgatagg atgaattttt ttaaaaggtt tatgcacggc agatatggaa tggatcagct
      241 ctcgttcaca cttttgatta ttgcattcat tatattaatc atttcaagac tgcttctccc
      301 tatacttggt tatttcgccc ttgccattgt tgtatacgca tattacaggt ttttttcaaa
      361 ggatataaaa aagaggcaag gcgagaatat gaagttttta agtgttgcaa agcctatgca
      421 gatgaaaata aaaaacaaaa ggcttttgat ttttggaacc aagacgcaca agtattacaa
      481 atgcccaaaa tgcaaacaaa tgcttagggt tccaaagaac aagggtttga tacagattac
      541 atgcagcaga tgcaaaaagg agtttattaa gaaaacctga tgaaatttag ctgtttttta
      601 agacgctgta tattgacaaa aacaacttca acctgttata atacataacg ttgtcacgct
      661 ggtgtggcga aataggcaga cgctatggac ttaaaatcca ttggtggcaa caccgtaccg
      721 gttcgagtcc ggtcaccagt accaatgagc agcgggcttt gtaaccgttg ctttttttat
      781 atattcatga atcgaggact tgatgtagat gaacagacag aaaagaattg tagcaataca
      841 cgacatatcc tgtgcaggac gctgctcgct gacagtagcc ctcccaatta tatcgagcgt
      901 gggcatagaa acgaatatta tacccactgc ggtattgtca acccagacag gtgatcttga
      961 agggtacact ttcaaggatc ttacagatca gattatgccc atagcaaatc attggaagtc
     1021 actggataga acttttgagg caatatatac tggatatttg ggttcttttg agcaaattga
     1081 ccttgttaag cagatttttt tgatgtttgg aaatgaagtt tgtttgaaat ttgtggatcc
     1141 agtaatggcg gatcatggag tattatatga cggctttgat tttgactttg taaaaggcat
     1201 gaaggactta tgcggacagg cggatattat aactccgaat attacagaag ccgtattcat
     1261 gcttggtgaa gaatatattg aaggaccgta cactgatgaa tacataaaca gtctgttgca
     1321 caaactcggc agcatatgta aaaaggttat tgtaacaggt attgatttta ctgataactg
     1381 ttatggtgct gcatcataca atgtagagga aggtgtgttt cagtacgcca agtctgagaa
     1441 agttcatggc aagtttcatg gaacagggga tgtttttgca tcatgcctta ttgccgcatt
     1501 gctcagtgat attcctatgt acgattcaat gaaaatggct gtcgatttta ctgtagaaag
     1561 cataaagcgc acaaaggaat caaaatccga tgtacgcttt ggaattaatt tcgaagctgg
     1621 tatttttgat tttgtaaaaa ggataagaaa ctttaaccca ggaaatccct tctaatactt
     1681 gcatattccg ttattgctat tattcctgca gtgttttcct cgctctttcc aatgtttgca
     1741 agataattct cataggaata aaaactgctt ggatcattct ttatataatc agaaattatg
     1801 ctgtcaattt catttaccct gttttcaaag tcttgtaaaa aattcttgca aatgtctaca
     1861 tactccagat acttgttgta atattcctca acacttagaa tcttgtatac aagtggtctt
     1921 tcagttatat ccgctctata tacaggactg tcaacaggag ttgcaacaga aactccatta
     1981 tccttctttt gagcaccgaa ggatagattg aaatcccatg gcaccatgta taatttgttg
     2041 tttacgttat acaaataata gttggtggcg gatgcaccta tatagctgtc atagttgccc
     2101 attacagtgt ttacagcaat gtatttgagg acgctgtcaa catgcataat attttcgatg
     2161 tcgccttttt ctcccaatgg catgctgttg agagtatcta tcaaaagttg caaatcttcc
     2221 ttggtttcat cactgccatt cttttgctcc ataagtgaaa gcgagttatg gtcaaccaat
     2281 gttgcctgtt catttgactt gtacagatta cctttgttgt tgccaaagtt tcttttgagg
     2341 aaactgtcgt ccacagactc aattgcaaca taaaagccaa caagattatc gtttatggta
     2401 actgtggcgt aggtagcaag gggtgctacc agctctacct tttcaaaagc ctcgcatagt
     2461 aaaaattccc tcatatagga tgggtctcta gacacatttg acagaaccag ttcatcaaga
     2521 ccatctatgg tttgaccttt cacaaacttg tcgaacttca gcctgtagct gtatctgtat
     2581 gcggactcgt caccaccgtc aagatccgta ttacccataa ttcttatgcc tgtgtttttg
     2641 atttcctgat tatttatttt aacagttgca gagtagtatt aataactatc gggatatgcc
     2701 cacatttctt ccagatatgc aggatttata gttattgaaa tctcaagaat atcattgtcc
     2761 ttgaaaatgt gttt
//
LOCUS       DAONVY010000314         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24868_length_2771_cov_3.675258, whole genome shotgun sequence.
ACCESSION   DAONVY010000314 DAONVY010000000
VERSION     DAONVY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24868_length_2771_cov_3.675258"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLI11_08205"
     CDS             complement(<1..620)
                     /locus_tag="PLI11_08205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPZ52885.1"
                     /translation="MGRFFVLPEQIKENTVEITGSDVNHIANVLRHSKGDNIMISSEG
                     FLYDCTIDEILKDRIVAIINKRTKMDTELNVEVVLYQGLPKGDKMDLIIQKNVELGAS
                     LIVPVIMERTIVRIEGKDVRKRVDRWNRIALEAAKQCGRAKVPEVLPPVFFKEFLKHS
                     KGKFKIMPYENENELHLKKFIGSIEKEEDLSFDVLIGPEGGISRQEA"
     gene            complement(705..1703)
                     /locus_tag="PLI11_08210"
     CDS             complement(705..1703)
                     /locus_tag="PLI11_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ52886.1"
                     /translation="MTASERIGKLIRGKEIDRLPVMEWAPWWHLTVARWISEGLPREN
                     AGYVELQDYFGLDKCIQTYFPVRTAMTPAAVPHGTGIMKSREDYERIKPTMFPDVTKI
                     VSKEHFDWLEKTRQKGDYVHFCTVEGFFWYPRELFGIEEHLYSFYDNDELLHEMCEDY
                     VSWLKKLFEFIGNSFRFDFMTFAEDMSYNKGPMISKDMFDTFLAPYYREIIPVIKRCD
                     IPVIIDSDGDITEAVDWYASVDADGMLPLERQAGVDVSLYIEKQPQMTFLGHFDKMCM
                     KFGEEAMRREFERILPSCIKGKVIIGVDHQTPPDVSLENYKVYVRLLKEYANKVVK"
     gene            complement(1766..>2771)
                     /locus_tag="PLI11_08215"
     CDS             complement(1766..>2771)
                     /locus_tag="PLI11_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52887.1"
                     /translation="FTFMIVSYLVRAAGDLGSEALSYLSPMGVAYRTYPYYKNNWLMI
                     ILLVLISLVFAGVALYLNSVRDHGAGLVASKPGRTHATRFLQSPLGLVLRISRTSTIA
                     WLVGIYVLGASYGSVLGDLDSFFETNEMFKLMLPQNADYTMTEQFITLILVILAMCGL
                     VPVLQLVLKIRGEEIRGRNEHIMARAVSRKSYLACYVFSSAILAFIMQFAAVVGLWSS
                     GSAVMDSPLSLGRMLANGLVYMPAALLLLGIAVFFVGCLPKASSAVWVYLGYCFFTEY
                     MGNLLKLPKWTKKLTPYGYTPKLPVDEMKPLNLVLITVIAFVLIWAGFAGFNKRDLEG
                     "
BASE COUNT          850 a          706 c          428 g          787 t
ORIGIN      
        1 gcctcctgcc ttgatatacc gccttcaggc ccaataagta catcaaaact caagtcttcc
       61 tctttttcaa tactgccaat aaacttcttc aaatgaagct cattctcatt ttcataaggc
      121 atgattttaa atttgccttt tgaatgcttc aaaaattcct tgaaaaaaac aggtggcaaa
      181 acctctggaa cctttgccct tccacactgc tttgcagcct caagggcaat cctgttccac
      241 ctgtccaccc tttttctaac atccttcccc tcaattctta caatagtcct ctccatgatt
      301 acaggaacaa tcaaagatgc accaagctcc acattctttt gaataattaa atccatcttg
      361 tcccctttgg gaagcccctg gtaaagaaca acttccacat tcaattcagt atccattttg
      421 gttcttttat taataattgc aacaatcctg tcttttaata tttcatcaat cgtacaatca
      481 tataaaaacc cttcagagga aatcataata ttatcgcctt ttgaatgcct caaaacattg
      541 gcaatatgat tcacatcact gcctgtaatc tcaaccgtat tctcttttat ctgctctggt
      601 agaacaaaga atcttcccac taaaatcacc tgttgaaaat tgaacaacct ttattttacc
      661 aaaaattctg cataaagtaa aacacggaac atacgttccg cattttactt tacaaccttg
      721 ttcgcatact cttttaacaa ccttacatac accttgtaat tctcaagcga cacatccggc
      781 ggagtctggt gatccacccc tataattact tttcctttta tacagcttgg aagaattctt
      841 tcaaactctc gccgcatggc ttcctctcca aacttcatgc acattttgtc aaaatgtcct
      901 aaaaaagtca tctgcggctg tttttctatg taaagactta catccacacc cgcctgcctc
      961 tcaagaggca gcatgccgtc tgcatcaact gaggcatacc agtcaactgc ctctgttatg
     1021 tctccgtcac tatctatgat tacaggtata tcgcaccttt ttataaccgg gataatctcc
     1081 ctgtaatatg gtgcaaggaa tgtgtcaaac atgtccttgg atatcatggg gcccttgttg
     1141 tagctcatgt cttctgcaaa ggtcatgaaa tcaaacctga agctattccc tataaactca
     1201 aatagttttt tcagccatga aacatagtcc tcacacattt catgcaatag ctcatcattg
     1261 tcatagaaac tgtacagatg ttcctctatt ccaaaaagtt ctctcggata ccaaaagaat
     1321 ccctcaacgg tgcagaaatg aacataatcg cctttctgtc ttgttttttc aagccaatca
     1381 aaatgctcct tggacactat tttcgtcaca tcgggaaaca ttgtaggctt tattctctca
     1441 taatcttccc ttgacttcat tatgccggtt ccatgaggca ctgcagcagg ggtcatggca
     1501 gtccttacag gaaaatatgt ctgaatgcat ttgtcaagac caaaatagtc ctgcagctct
     1561 acataacccg cattctctct gggaaggcct tcacttatcc acctcgctac cgtaagatgc
     1621 caccagggag cccactccat taccggcagc ctgtctattt ccttccctct tatcaatttc
     1681 cctattcttt cacttgcggt catatcattt actcctctta acaaaactaa atatataata
     1741 tacccgaaag aaaaggaata caagactatc cttccaaatc ccttttgtta aaccctgcaa
     1801 atcctgccca aatcaataca aatgcaataa ccgttattaa aaccagattt aaaggcttca
     1861 tctcgtccac cggcaatttg ggagtatatc cataaggggt aagttttttg gtccatttgg
     1921 gcagctttag caaattaccc atatactcag taaagaagca atatccaaga tacacccaaa
     1981 cagcgctcga tgcttttggc aggcaaccta caaaaaagac agcaataccc agcagcaata
     2041 acgcagcagg catgtataca agcccgttag caagcattct tcctaaagat agcgggctgt
     2101 ccataacagc actaccggaa gaccacaatc caacaacagc ggcaaactgc ataataaagg
     2161 caagaattgc cgaagaaaac acatagcagg caagatagct ttttcttgac acggctcttg
     2221 ccataatatg ctcgtttctt ccccttattt cttctcctct aatctttaaa accagctgaa
     2281 gtaccggtac aaggccgcac atggccagaa taaccagtat caatgtaata aactgttctg
     2341 tcatagtata atcagcattc tgaggaagca taagcttgaa catttcatta gtttcaaaaa
     2401 aggagtccaa gtcacccaac actgaaccat aggaagcgcc cagcacataa ataccaacaa
     2461 gccatgcaat agtggaggtt cttgatattc taagcaccag acccaaggga ctttgcaaaa
     2521 atcttgtagc atgcgtcctt ccaggctttg aggcaacaag acctgctcca tggtccctga
     2581 cagaatttag ataaagtgcc acaccagcaa acactaaaga tattaatacc aaaagtataa
     2641 tcattaacca gttattcttg taatatggat aggtgcggta agcaactccc ataggcgaca
     2701 gatatgacaa agcttcgctg cctaagtctc ctgcagcacg cacaaggtaa gagactatca
     2761 tgaatgtaaa g
//
LOCUS       DAONVY010000315         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_24937_length_2765_cov_3.653506, whole genome shotgun sequence.
ACCESSION   DAONVY010000315 DAONVY010000000
VERSION     DAONVY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24937_length_2765_cov_3.653506"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLI11_08220"
     CDS             complement(<1..993)
                     /locus_tag="PLI11_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPZ52888.1"
                     /translation="MNITLHLTNSCNLKCSYCFVDKCERFMDRMTAFKAVDFVVRRSL
                     EKENIGISFYGGEPLLMKNLIYDTVLYSKARYPDRRFSFSLTTNGLLLDEQMCLFAKN
                     FGLLIAISIDGDKNVHDMCRVDLNSQGSHERVVNAAKMLLKYKPNAVCMATVNPETAP
                     YLSDSVKYLYDLGFTYVVCTINHFAPWTDEDLRVLKEEYGRIADLYYEKTIKEDKFYF
                     APFDIKISNYIFNKSSKETCSLGERHISVTPEGDLYPCLQLVDRDEYHLGHLDTGISD
                     EKRKKVHELSKSIASDCEGCTLYDRCRNYCSCANISASGYIDKVSPLQCEHERMI"
     gene            complement(990..1649)
                     /locus_tag="PLI11_08225"
     CDS             complement(990..1649)
                     /locus_tag="PLI11_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52889.1"
                     /translation="MKICKARRYNAGYPTKEETLIYPELLKNMPERWKGLLVSGTLFS
                     SVLLASCDPRTLFNRTVPVVLAGDAYVTLTFAEDEGVEIIIDEFLAQGIEFEHAYRVK
                     VNAELPVDVEYEEEFEYHTLKAELEVDGMSKDGTIYFEFVSHHDFSKWTDDKVLIDDY
                     FTRGEFLKEFFEDGMFETENGEIVAAFYNTQGFDYGSMEEELRQQVRDFIKMMEEQGI
                     L"
     gene            complement(1679..2362)
                     /locus_tag="PLI11_08230"
     CDS             complement(1679..2362)
                     /locus_tag="PLI11_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52890.1"
                     /translation="MQIHKVKKYNAGYPKREETLKNPNLLKNAPDRWKALLASGTILS
                     SVILASCGINSPQDLWNKIKPTPTPPLIEGVVISIPYYYIDEEAIDIIVDEFKAYGIM
                     FDSENTKRVVAELPVDVKSIGDSMEFEYYTLTSEFIVDGTREDLSISFEFISKEDFSK
                     WAGQDNMYPYYSTTFLRGFLEDGKFNTKNDEIVLAFYSKHTNVDNMEKDLRKQVRDFI
                     QMLEYQGVI"
     gene            complement(2380..>2765)
                     /locus_tag="PLI11_08235"
     CDS             complement(2380..>2765)
                     /locus_tag="PLI11_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52891.1"
                     /translation="FDTVNTDKVVAELPVDVKFDVRNDKFKYYTLPTGLNVDGINKDY
                     KISFEYVSKEDFSKWTTDENMNSEHIKGKFLDEFLEEGIFETKNGELVMAFYNSSHHD
                     PTWLEEELRQQVREFVQMLQEQGVL"
BASE COUNT          806 a          639 c          430 g          890 t
ORIGIN      
        1 aatcatcctc tcatgctcgc attgaagtgg tgacactttg tcaatatatc cacttgcact
       61 aatgttagca caagaacaat agtttctgca cctgtcatac aaagtgcacc cctcgcaatc
      121 agaggcaata cttttgctta gttcatgaac cttttttctt ttctcatctg aaatacccgt
      181 atccaaatgc cccagatgat attcatcccg gtcaacaagc tgaagacatg gatacaaatc
      241 tccctcggga gttactgaaa tatgcctttc tcctagtgag caggtttcct ttgaactttt
      301 gttgaaaata tagttgctga tttttatatc aaagggagca aaatagaatt tgtcttcctt
      361 tattgtcttt tcataataca aatccgcaat cctgccatac tcttccttta acactctcaa
      421 atcctcatcc gtccagggtg caaaatgatt tatcgtgcaa acaacatagg taaatcccaa
      481 atcatacaga tactttaccg aatcagaaag ataaggtgca gtctccggat ttacagttgc
      541 catgcaaact gcattcggtt tgtactttaa aagcattttt gcagcattaa caaccctttc
      601 atggcttccc tgactgttta aatccactct gcacatatca tgcacatttt tgtcgccatc
      661 gatactgatg gctataagga gcccgaaatt ctttgcaaac aggcacatct gctcatccaa
      721 taacagacca ttcgtagtca gactgaagga aaaacgtctg tcaggatacc ttgctttgga
      781 atacaagaca gtatcatata ttaaattctt catgaggagg ggctctcctc cataaaagga
      841 gatgccaatg ttttctttct caagggaacg tcgaactaca aaatcgacag ctttaaaagc
      901 tgtcattctg tccatgaatc tttcgcattt gtctacaaag caatatgaac atttcaaatt
      961 gcagctgttt gttagatgta atgtaatatt cataaaatac cttgctcttc catcatttta
     1021 ataaagtctc ttacctgctg acgaagctct tcttccattg aaccgtagtc aaaaccttga
     1081 gtgttgtaaa atgcagcaac tatttcacca ttttccgtct cgaacatgcc atcttcaaaa
     1141 aactccttca ggaattctcc tcttgtaaaa taatcgtcta tcaagacttt atcatccgtc
     1201 catttggaaa agtcatgatg agacacaaat tcaaagtaga tagttccatc cttgctcata
     1261 ccatccacct ctagttctgc ctttaaggtg tggtactcga attcttcttc atactccaca
     1321 tccacaggca gctcggcatt tacttttact ctataggcgt gttcaaactc gataccttga
     1381 gctaaaaatt cgtcaattat tatttcaaca ccctcatcct ctgcaaaagt cagagtaaca
     1441 tacgcatctc ccgccaatac aacaggaact gttctattaa aaagagttct tggatcgcag
     1501 cttgccaaaa gaacagatga aaaaagagtg ccagagacca aaagcccttt ccatctttca
     1561 ggcatgttct ttagtaattc gggatagatt aatgtctctt cctttgtagg ataccctgca
     1621 ttgtatctcc tcgccttaca gattttcata gtaatctccc ttaccaaatt aaattatatt
     1681 atatcacacc ttgatattcc aacatttgaa tgaaatctct tacctgtttt cttaaatcct
     1741 tttccatatt gtcaacatta gtgtgtttgc tataaaatgc aagaactatc tcatcgttct
     1801 tcgtattgaa ctttccgtcc tcaaggaatc ccctcaagaa ggtcgttgag tagtaaggat
     1861 acatgttatc ctgcccggcc catttggaaa agtcttcttt ggatatgaat tcaaaagaaa
     1921 tactaaggtc ctcccttgtt ccgtcaacaa taaattcaga agtcagagta taatattcga
     1981 actccatgga atctccaata ctttttacat ccacaggcag ctctgcaaca accctctttg
     2041 tattttcaga atcaaacata ataccataag ctttaaactc atctacaata atgtctattg
     2101 cctcttcatc aatgtaataa tacgggattg aaattacaac tccctcaatt aaagggggag
     2161 ttggtgtagg ttttatttta ttccataagt cctgcggact gtttatacca caactggcaa
     2221 gtattactga tgacaggatt gtaccggaag ccaataacgc tttccatctg tcaggggcat
     2281 tctttaatag attgggattt ttcaaagtct cttccctttt agggtaacct gcattgtatt
     2341 ttttaacttt atgtatttgc atatcctgcc cccgaatctt cacaatactc cttgttcctg
     2401 tagcatctga acaaactctc tcacctgctg cctgagttcc tcttccagcc atgtagggtc
     2461 atgatgcgaa ctgttgtaaa aggccatgac aagttcacca ttctttgttt caaaaattcc
     2521 ttcttccaag aactcatcaa gaaattttcc tttaatgtgt tcagagttca tgttctcatc
     2581 agttgtccat ttggaaaaat cctcttttga tacatactca aaagaaattt tgtaatcctt
     2641 gttgatgccg tccacattta gcccggtggg aagggtgtaa tatttgaatt tatcgttcct
     2701 aacatcaaac ttcacatcca ccggcagctc cgccacaacc ttgtcagtat tgactgtatc
     2761 aaaag
//
LOCUS       DAONVY010000316         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25140_length_2748_cov_2.999257, whole genome shotgun sequence.
ACCESSION   DAONVY010000316 DAONVY010000000
VERSION     DAONVY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25140_length_2748_cov_2.999257"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..1429)
                     /locus_tag="PLI11_08240"
                     /pseudo
     CDS             complement(401..1429)
                     /locus_tag="PLI11_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1464..2012)
                     /locus_tag="PLI11_08245"
     CDS             complement(1464..2012)
                     /locus_tag="PLI11_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPZ52892.1"
                     /translation="MSGFAIGAKIRELRIRNGLTQEELANRSELSKGFISQIESEQTS
                     PSIATLVDILECLGTNLRDFFNEATEEQIVFTKKDYFETENLDQGNKITWLVPNCQKN
                     DMEPIILTLEPGGKSSEEEPHNGEEFGIVLQGTVMLHIGDKAYKVKKGESFYFLPFSR
                     HYIANNTTKSAKVLWVVTPPSF"
     gene            complement(2215..>2748)
                     /locus_tag="PLI11_08250"
     CDS             complement(2215..>2748)
                     /locus_tag="PLI11_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ52893.1"
                     /translation="IFDTYLTCCIKYFDMVWDRGFKFDSIYWPDDMGYKNTTFFSKDL
                     YEKLLKPFHKRAVDYAHEKGVFAHLHSCGNVMNFLPDIVEIGVDILNPLEVKAGMDPL
                     RIKKAYGEKLSLHGGVNAVLWSDSEKIIEEIKRVVPKLKENGGYIFASDHSIPNSVSF
                     ENFSRIISTVKEVGSYN"
BASE COUNT          734 a          670 c          454 g          890 t
ORIGIN      
        1 cattctcaaa atcgcctcct tcatatacgc tcatctcgtc cttgtcgtgg acatccaccc
       61 tcttgcggat cttgatgctc tctcttggta tatagaggca tacttccacg cctattgcca
      121 tcttcctgtc agtgtgaaca agaaaatcag tatcggaaca tcttacaata acttcatagt
      181 caagcccctt gtcttttaca ttgacaatct cgccgttgat tgttccacca ggcatttccg
      241 tctcaaaatt cttctcctta atctctatat cctgtgggag aatatatacg cttactgtct
      301 catcctgctc aaaaccgctg gcattacagt caaacatgct gtcgacgatt tccactttgt
      361 tatcctcaac catccatccc gtcatgatat tataagggat tctcataata tgtatgtcat
      421 agggatctat gcagaggccg accacgctgc caggttttga atatgccgtg gtgtgaactt
      481 tccatttgaa tccattggct tcaacagtga tttcatagtg gacaccttta aacagagtgc
      541 tcaaaacctc tccctgcagc atgccttttt caggctccac aatctttata tcctcaggtc
      601 ttactaccac gtccacatcc tcgtcttctc caaaaccctt gtcctcgcat tgaaattcct
      661 tgtttgcaaa ctcgcaaagg tagtctcttt tcatgacgcc ggaaagaatg ttgctttcgc
      721 caataaactg ggcaacaaat gcattgctag gctcattgta tatgtccgtg ggctttccta
      781 tctgctgaat aacaccatga ttcataacaa taatggtgtc ggacattgta agggcttctt
      841 cctgatcatg ggttacatat acaaaggtta ttcccgtctt cttttgaagg ttcttaagct
      901 catactgcat ttccttgcgc aacttgagat caagagctcc aaggggttcg tccagaagaa
      961 gtacctcagg ttcatttaca attgctcgtg caatggcaat tctctgctgc tgtcctccgc
     1021 tcaatgaatg gggcattctt ttttcaaaac cggacaattc aaccagttcc aatactcttg
     1081 cgacattttc ctttatatcc gtttccttca tcttctttat tctcaggccg aaagcaatgt
     1141 tctcatagat attcatgtgt ggaaacaaag aatatttttg aaatacagta ttaataggtc
     1201 ttttatgcgg aggcacgtca gtaatatcca cattattgaa gaatatgcgg cctgaatcag
     1261 gttgctcgaa tccaccaata atacgcaggg ttgttgtctt gccacagccc gacggaccaa
     1321 gcagagtcaa aaactcattc ttcctgatgt ataatgtcaa atcctttata acttcagtat
     1381 ctccatagct ctttgtaatg ttgtacaagt caattatatg ttccgacatc aagatccccc
     1441 ttattcatca tatcatatat tctctaaaag ctgggaggtg taactaccca tagcactttt
     1501 gcactttttg ttgtgttgtt cgctatataa tgcctggaaa aaggtaaaaa ataaaagctc
     1561 tcgcccttct tcaccttgta tgccttatca ccaatatgta gcataaccgt tccttgcaaa
     1621 actattccaa actcttcccc gttgtgaggc tcttcttccg aggacttccc accaggttcc
     1681 agagtaagta tgataggctc catatcgttc ttttgacagt tgggcactag ccatgttatt
     1741 ttgtttcctt ggtcaagatt ctcagtttca aaatagtcct tttttgtgaa aacaatctgt
     1801 tcttcggttg cttcattaaa aaaatccctt aaattggtcc ccagacactc aagtatgtct
     1861 accagtgttg caatcgaagg tgatgtctgt tcactttcta tttgagagat gaaacctttt
     1921 gacaactcgc ttctgtttgc cagctcttcc tgtgttagcc cgttccttat cctgagctcc
     1981 cttatctttg ctccaattgc aaaccctgac atgggaaata tccctccttt tcgtttctac
     2041 gagttttact gatagtaaac tttaagttta cctttactaa actcacgacg gatattgtac
     2101 taacatttta gccgcatgtc aactgttttt caagatttaa cattctatat acaatttata
     2161 taaaaacagc ctccggatcg ctccgaaggc atttaaaaca atataaaccg ataactaatt
     2221 gtatgaacca acttccttta cagtactgat aatccttgaa aaattctcaa aactcacgct
     2281 atttggaatt gaatgatccg atgcgaaaat ataacctccg ttttccttca acttgggaac
     2341 taccctttta atctcctcaa tgattttctc agaatcactc caaagtactg cattaactcc
     2401 cccatgcaga ctcagcttct cgccgtacgc cttctttatc cttaaggggt ccattcccgc
     2461 cttgacttcc agagggttca gtatatccac ccctatttcc acaatatcgg gcaaaaagtt
     2521 cattacattg ccgcaggagt gcaaatgtgc aaatacaccc ttttcatgtg cgtaatccac
     2581 agctctcttg tgaaaaggct ttaggagctt ttcataaaga tccttggaaa agaaagtggt
     2641 gtttttgtat cccatatcat ccggccagta gatggaatca aatttaaaac ctctatccca
     2701 aaccatgtca aaatatttga tgcagcaggt cagatatgta tcaaaaat
//
LOCUS       DAONVY010000317         2742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25201_length_2742_cov_3.040566, whole genome shotgun sequence.
ACCESSION   DAONVY010000317 DAONVY010000000
VERSION     DAONVY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25201_length_2742_cov_3.040566"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1465
                     /gene="atpD"
                     /locus_tag="PLI11_08255"
     CDS             68..1465
                     /gene="atpD"
                     /locus_tag="PLI11_08255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPZ52894.1"
                     /translation="MGNGRIVQVSDSVVDVEFKHGNLPKIYDALYVNVNGETRYMEVA
                     QHVGENTVRCIMLAESNELARNMEVKATGSGISVPVGRCTLGRMFNVLGEPIDGKEPV
                     SEAETRWEIHRKAPAFESQRPNFEILETGIKVIDLLEPYTKGGKIGLFGGAGVGKTVL
                     IQELIHNVAMKHGGYSVFTGVGERSREGNDLFREMQQSGVLEKAVLVFGQMNESPGVR
                     MRVGLTGITMAEYFRDCENKDVMLFIDNIYRFAQAGSEVSTLLGRMPSAVGYQPTLAE
                     EMGKLQERITSTEKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKVAEQGIY
                     PAVDPLNSTSRILEPDIVGKEHYQIARKVQEILQKYVELQDIIAILGMDELGDEDRLV
                     VARARRIQRFLSQPIHVAEKFTGLSGVYVPLEETLRGFKAILNGDMDTYPELAFFNVG
                     TIDDVMKKAKEMGHE"
     gene            1468..1887
                     /gene="atpC"
                     /locus_tag="PLI11_08260"
     CDS             1468..1887
                     /gene="atpC"
                     /locus_tag="PLI11_08260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPZ52895.1"
                     /translation="MNTFNLRMLAVDRPFYSGPCESIIVPTPEGKYGIMANHLNMVIA
                     ITPGALIFRIPDKENEIVAVSHGFVKVENNNVLVLVDSIERAVEIDVNRAKRAAAAAK
                     EAILQNRNMQEYLAAQARLARALNRLRVKGFYDIKMK"
     gene            1931..2569
                     /locus_tag="PLI11_08265"
     CDS             1931..2569
                     /locus_tag="PLI11_08265"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HPZ52896.1"
                     /translation="MSRKFIVLVIGIFFSSFGVGLSLKSNLGATPIGVCPAVFSDAVN
                     ISTGTAMAVLLGIFFVMQILILKKDFRPFQLLQLFATLIYGVFVDLSEVILSYFSNDE
                     LWQRSIYCVLGFVILAFGVFVMLSTDFIMLPQDAAVAVISNKYKKEYGKIKITLDVIL
                     TTIATASSFVIFSRIIHVGVGTILAAIFVGKIISLLQKSAGINRFISWAIGD"
BASE COUNT          842 a          441 c          667 g          792 t
ORIGIN      
        1 aaataactga agtgacagcc agcgcaaaag ctcagataag aaaacggaaa aaggaggtgc
       61 tttaatcatg ggtaatggaa gaattgtgca agtgtcggac tccgttgttg atgtggagtt
      121 caaacatgga aatctgccta agatatatga tgctctatat gtaaatgtaa atggtgaaac
      181 aagatacatg gaagttgccc agcatgtggg tgaaaataca gtgcggtgca taatgctggc
      241 ggaaagcaat gagcttgcca gaaacatgga agtaaaagct acggggagtg gaattagtgt
      301 gcctgtaggg agatgtactt tgggaagaat gttcaatgtt ctaggtgaac ctatagatgg
      361 aaaagagccc gtttctgagg ctgaaactcg gtgggaaatc cataggaagg cacccgcttt
      421 tgaaagccag cgtcccaatt ttgaaattct tgaaactggt ataaaagtaa ttgacctact
      481 ggagccatat accaaaggcg ggaagattgg gctgtttggc ggtgctggtg ttggcaaaac
      541 cgttctgatt caggaactga tacataacgt agcaatgaaa catggcggtt attcagtttt
      601 tacaggcgtt ggtgaaagaa gccgtgaagg aaacgacctc tttagagaaa tgcagcaaag
      661 cggtgttctt gaaaaggcgg ttctagtgtt tggacagatg aatgaatctc ctggtgtgcg
      721 tatgcgtgtt ggacttaccg gcattacaat ggctgaatat ttcagggatt gtgagaacaa
      781 ggacgtaatg ctgtttattg acaatatcta cagatttgct caggcaggaa gcgaggtttc
      841 cactctgctt ggcagaatgc cttccgctgt tggatatcag cctacattag ctgaagaaat
      901 gggcaagctt caggagagaa ttacgtccac tgagaagggt tccataacat cggtacaagc
      961 cgtatatgta ccggcggatg accttacaga ccctgctcct gcaacgactt ttgctcattt
     1021 ggatgctaca acagtgttaa gcaggaaggt tgcagaacaa ggtatttatc ctgcagtaga
     1081 tcctcttaat tccacatcaa gaattctgga accggacatt gttggtaagg agcattatca
     1141 aatagctaga aaagttcagg aaatacttca aaagtatgtt gaacttcagg acataatagc
     1201 gatactggga atggatgaat taggagatga agacagactt gttgttgcaa gagcaaggag
     1261 aattcaaagg tttttatccc agccgataca tgtagctgag aaatttacag gattgtccgg
     1321 tgtgtatgtt ccacttgagg aaactttaag aggttttaag gctatactga atggtgacat
     1381 ggatacttat cctgaattgg cgtttttcaa tgtgggaaca attgatgacg tgatgaaaaa
     1441 ggctaaagaa atggggcatg aatgattatg aacacattca atttgcgcat gttggcagtg
     1501 gatcgcccct tttattctgg tccatgtgaa tcaattattg tgcctacacc agaaggcaaa
     1561 tatggcatta tggcaaatca tttaaacatg gttatagcta taacgcctgg ggctttaatt
     1621 ttccgtattc cagacaaaga gaatgagata gtagcagttt cacatggatt tgtgaaagta
     1681 gagaacaata atgtcttggt tttagttgac tcgatagaac gggctgtaga aattgatgta
     1741 aatagagcga aacgtgcagc tgcagctgcc aaagaggcta ttcttcagaa tcggaacatg
     1801 caggaatatc tggctgctca ggctcgcctt gctcgtgctc ttaacaggct gagagtaaaa
     1861 ggtttttatg atattaaaat gaagtaatag aactggaggt atcaagaaag atagtatgga
     1921 tttgaaaacc atgtctagaa aatttattgt attagtgata ggcatatttt tttcttcatt
     1981 cggagtagga ttatcactta aatcaaatct tggtgctaca cccataggtg tatgtcctgc
     2041 ggtgttcagc gatgctgtca acatatcaac aggaacggca atggctgtat tgctgggcat
     2101 attctttgta atgcaaatac ttattctaaa aaaggatttt cgcccatttc aattgctcca
     2161 actctttgcc acactaatat acggtgtctt tgtggacctt tcggaagtga ttctttcata
     2221 tttttcaaat gatgaactgt ggcaaaggtc tatttattgt gtgttgggct tcgttattct
     2281 tgcttttgga gtattcgtaa tgttgagtac ggattttatt atgcttcccc aggatgcagc
     2341 cgttgcagta atttcaaaca agtataaaaa ggagtacggt aagataaaga ttactttgga
     2401 tgtaatactg actacaattg caactgcaag ttcttttgta atattcagta gaataataca
     2461 tgtgggcgtt gggaccattt tagctgcaat cttcgttggc aagataatca gcctgcttca
     2521 gaagtctgct ggtattaatc gttttattag ctgggctatt ggtgattaaa ggaaaaatgg
     2581 ttttcataaa gtggacaaac ttttgtcaat atgctagaat aacctgatgt tatacataca
     2641 gaattacttg aattaagcga ggagaatgaa catgagtaaa ctgcttatcc ataacggaaa
     2701 agttcttacc ataactggaa aagaatacga aaatgggtat at
//
LOCUS       DAONVY010000318         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25475_length_2716_cov_3.446449, whole genome shotgun sequence.
ACCESSION   DAONVY010000318 DAONVY010000000
VERSION     DAONVY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25475_length_2716_cov_3.446449"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLI11_08270"
     CDS             <1..584
                     /locus_tag="PLI11_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HPZ52897.1"
                     /translation="VHPTTGAAQFVEVRERVEEIRVQKVVEVDSSLERIETNPQYAQI
                     AATNEQIAIVTMNVKISNVEGFIHFCLPHLAIKPIEKLLSVKSMYNVYGEQKSDEMSE
                     QIKDLIDTTKLNLVARFNNTYSCVKDVMDIQEGDVIVLDHRVDNPINLYVEHLPKFKG
                     KLGVKDRRYSIMINDIEYEEDNIDEWDFISSGN"
     gene            553..1647
                     /gene="fliY"
                     /locus_tag="PLI11_08275"
     CDS             553..1647
                     /gene="fliY"
                     /locus_tag="PLI11_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HPZ52898.1"
                     /translation="MSGILSQAEIDALLKSNSVNITDDEIDTLGEIGNISMGTAATTL
                     YEILGKRVDITTPKVSVTTMRKMAETYTIPYVGIEVKYLSGVEGFSFLVLKMVDALII
                     TDLLMGGDGTNPGEELDEIRLSALGEIMNQMMGSAATSMSEMLGTSVTIAPPKLFEVR
                     FADTKLDNILEDDEIVKISFKMVVEDIINSEIMQIMSKDYAIKFAGKLLNTGEEPVEN
                     PDTISETEEVKVEAKADREEGKKEKKATRQPSAKVNVSPLELKSFEDEDEEEKSEEVD
                     VGDNLGLLMDVPLPITIELGRSKKMIKEILELNEGSVVVLDRFAGDLVDVVVNGKLIA
                     KGEVVVVDENYGVRITEVLSKKSVIRKERA"
     gene            1651..1977
                     /locus_tag="PLI11_08280"
     CDS             1651..1977
                     /locus_tag="PLI11_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPZ52899.1"
                     /translation="MPELLYIFVFVLVLIGCFFVTKWISVRSYTLVKGRNLRVIERIP
                     VDRDKSIILLARGEKVYLIGVAPNSINVLDTFSKDEIMEIEPMQVKIPFDKVLRRFVN
                     EKDEED"
     gene            1955..2716
                     /gene="fliP"
                     /locus_tag="PLI11_08285"
     CDS             1955..2716
                     /gene="fliP"
                     /locus_tag="PLI11_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689173.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPZ52900.1"
                     /translation="MKKTKKIKKTTFLVSTAFVLIALSLLLPGIAFAQEEPIPFPEVE
                     NSSSVVNIILLLTILSIAPAILLMMTCFTRIIIVLSFTRSGIGAQQMPPNQVLIGLAL
                     FLTFFIMQPVLSEINREAYRPLMQGEINTQEALERGKKPLKEFMLKNTYSQDLNLFIE
                     LSGKDAPETYDELPMTTVVPAFAISELKRGFQMGFFILIPFIVIDMIVASTLMSMGMM
                     MLPPTMISLPFKVLLFVLVDGWSLTVKTLVESFGG"
BASE COUNT          844 a          468 c          686 g          718 t
ORIGIN      
        1 aggtgcatcc caccacgggc gcggcgcagt tcgtggaagt gcgcgagcgc gtggaggaaa
       61 tccgtgtgca gaaggtggtg gaggtggact cctctttgga gaggatagaa acaaacccgc
      121 agtatgcaca gattgcagcc accaacgagc agattgccat agttacaatg aatgtaaaga
      181 ttagcaatgt ggaaggcttt atacattttt gtcttccgca cctggctata aagcctatag
      241 aaaagctgct ttcggttaag tccatgtaca atgtatacgg agaacagaaa tcagatgaaa
      301 tgtctgaaca gataaaagac cttatagaca cgaccaagct gaacctggtg gcaaggttca
      361 ataacacata cagctgtgtt aaggacgtta tggatataca ggaaggagat gtaattgttc
      421 ttgaccacag ggtggacaat ccaataaatc tgtatgtgga acatctgcca aagtttaagg
      481 ggaaattggg agttaaagat agacggtatt caattatgat aaatgacatt gagtatgagg
      541 aggacaatat agatgagtgg gattttatct caagcggaaa ttgatgcatt gttgaaaagc
      601 aattcggtaa atattactga tgatgagata gatactttag gcgaaatagg gaatataagt
      661 atgggaactg cggcgacaac cctttatgag atactaggaa aaagggttga cataaccacg
      721 ccgaaagttt ccgttacaac catgagaaaa atggctgaaa cttacaccat tccctatgtg
      781 ggaatagagg taaagtacct ttcaggtgtt gaagggtttt cattccttgt tctcaaaatg
      841 gtggacgccc taataattac ggatcttctg atgggtggag atggaacgaa tccgggagaa
      901 gaactggatg aaatacgtct ttccgcactt ggtgaaatca tgaaccagat gatgggatct
      961 gctgcaacga gcatgtcgga gatgctgggc actagtgtca ccattgctcc tccaaagctc
     1021 tttgaagtta gatttgctga caccaaactc gacaatatac ttgaagatga cgagattgta
     1081 aaaataagtt tcaagatggt tgtggaagac ataatcaaca gcgagataat gcagattatg
     1141 tccaaggatt atgccataaa atttgccgga aaacttctta atacgggaga agagcctgtt
     1201 gagaatccgg atacaatatc ggaaacggag gaggtaaaag tggaggcaaa agccgacagg
     1261 gaagagggaa agaaagaaaa gaaagcaaca aggcagccat ctgccaaggt aaacgtatct
     1321 cctcttgaac tcaagtcttt tgaagacgag gatgaagagg agaagagtga agaagttgat
     1381 gtgggagaca atttgggtct tcttatggat gtgccactgc ccattaccat tgaactggga
     1441 agaagtaaaa agatgattaa ggaaatactt gagcttaacg aaggttcggt agtggttctg
     1501 gacagatttg caggagattt ggttgatgtg gtggtaaacg gcaagctgat tgcaaaaggc
     1561 gaagtcgttg ttgtggacga gaattatggt gtaaggataa cggaagtttt atctaaaaaa
     1621 agcgttatca ggaaggaaag ggcgtaagaa ttgcctgagt tgttgtacat atttgtattt
     1681 gtacttgtgc tgattggatg tttctttgtt acaaagtgga tatcagtcag gtcatatacc
     1741 cttgtaaaag gtagaaactt gagagtcatt gaaagaatcc ctgtggacag ggacaaatcc
     1801 atcatactcc ttgcaagggg cgaaaaggtg tatcttattg gtgttgcgcc aaacagtatc
     1861 aatgttctgg ataccttttc aaaggatgag ataatggaga ttgagccaat gcaggtaaag
     1921 attccttttg acaaagtact aaggaggttt gtcaatgaaa aagacgaaga agattaaaaa
     1981 gacaaccttt cttgtatcaa ccgcatttgt cctgattgcc ttgtcattgc ttctccctgg
     2041 tattgcattt gcgcaggaag aacctattcc ttttccagag gtggagaact cctccagtgt
     2101 ggtcaatatt attctacttt tgaccatcct ttccattgca cctgcaatac ttttgatgat
     2161 gacctgcttt acgaggatca taattgtgct ctcctttaca agaagtggaa taggagcaca
     2221 gcagatgccg cctaaccagg tgctcatagg gcttgccctg ttccttacat tttttataat
     2281 gcagcctgtt ttgtcagaga ttaacagaga agcatacaga cctttaatgc agggagagat
     2341 aaatactcag gaggctctag aaagagggaa aaaacccctc aaggagttca tgctgaagaa
     2401 tacatacagt caggacctca acttgttcat tgaactttcg ggaaaggatg caccagaaac
     2461 ctacgacgag cttccgatga ccactgtggt tcccgccttt gccattagcg agcttaaacg
     2521 aggcttccaa atgggatttt tcatactaat acctttcatt gttattgaca tgattgtagc
     2581 aagcacgctg atgtcaatgg gtatgatgat gcttccgccg accatgatat cgctgccgtt
     2641 taaagtgctt ttgtttgtgt tggtggatgg ctggagcctt acagtaaaaa cattggtaga
     2701 gagttttgga ggttga
//
LOCUS       DAONVY010000319         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25509_length_2713_cov_3.191121, whole genome shotgun sequence.
ACCESSION   DAONVY010000319 DAONVY010000000
VERSION     DAONVY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25509_length_2713_cov_3.191121"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLI11_08290"
     CDS             complement(<1..293)
                     /locus_tag="PLI11_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52901.1"
                     /translation="MDRKTFIKYAVLVIGSALVGFILLNTLSLLFDNKGVYVATATGH
                     VAVVLLNDEKGLGYNELLASYELANSYLNYVNDRYMYKKAVNNLPDGLSKEYT"
     gene            complement(300..920)
                     /locus_tag="PLI11_08295"
     CDS             complement(300..920)
                     /locus_tag="PLI11_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52902.1"
                     /translation="MKRIKTRHIIIIVLSSFIFSLISWVVQKTVIAKEYVARTTVAVK
                     TQETISSYSQLSVSHYMAETFAGVFASNDVIAATADSMNMSFDEVREKVKVTRKKHTV
                     LIKIDARDVNPNKAIQLSKEFTDSLNENLAKPLGLHVEIIEEANEVIQVSGGLRALLA
                     GAAIGAVLGFLYVFLKTYLSDRFLDEERFQRLGVPVLGTALVDKRS"
     gene            complement(907..1182)
                     /locus_tag="PLI11_08300"
     CDS             complement(907..1182)
                     /locus_tag="PLI11_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPZ52903.1"
                     /translation="MKIKDGYMLREIDGVSVVVAVGEESKRFNGMIRLNSTGTFLWKH
                     LSEGIEYDDLLDKMLAAYEVDEQTARQGIDSFITKLKDNGFLSDEKN"
     gene            complement(1197..1493)
                     /locus_tag="PLI11_08305"
     CDS             complement(1197..1493)
                     /locus_tag="PLI11_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52904.1"
                     /translation="MKKFLALFIIVLSISLLVSCGDDKKDVKTTPTPEATDEGIDFND
                     LLDNTKTPGSATSTPETSTSTPSQSTSTPQPTESDTPGATDVPDYDDDNWTGLY"
     gene            complement(1614..>2713)
                     /locus_tag="PLI11_08310"
     CDS             complement(1614..>2713)
                     /locus_tag="PLI11_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPZ52905.1"
                     /translation="GAFFGWLIGKYIGKLEISFTGRIMSFFVMIILMYFAIFIQIVIH
                     ELGHLIFAFMTGYKFSSFRIMSLMLIKESGKIKLRHFSVAGTGGQVLMAPPDMKEEKF
                     PVVLYNLGGSILNTLTGLLFLFSHFKVLHMPRLSLFLMFLASSGIVLALLNGIPMRMG
                     MMDNDGYNALTLTKNKESRRSFWLQLKINELLANGVRLKDMPDEWFEIPSDEEMKNSM
                     MAVIGVFACNRLMDNHKFDEADKLMTHFMNIESSIVALHKGILVCDRIYCELVNENRK
                     NVVDDMLDARQKKLMKAMRKFPNVIRTEYAYALLAEKDLKKAQEIRKKFDRYTRYYPY
                     SVEIETELELIEIANRCKNLENIDLKEDHGA"
BASE COUNT          827 a          622 c          414 g          850 t
ORIGIN      
        1 tatgtatatt ccttggaaag tccatcagga agattattaa ctgctttttt atacatatac
       61 ctgtcgttta catagttcaa gtaggaattt gcaagttcat atgaagccaa aagctcattg
      121 tatccaagac ctttttcgtc attaagcagc acaacagcca catgccctgt agcagttgca
      181 acataaactc ccttgttgtc aaacaacagg gataaagtat taagtaatat aaaaccaact
      241 agtgcagaac caattaccaa cactgcatat ttgataaagg tctttctatc catctaccat
      301 tagctcctct tgtcaaccag ggcagtacct aatacaggta ctccaagtct ttgaaatctc
      361 tcctcatcca aaaacctatc agacaaatat gtttttagaa aaacatataa aaatcctagc
      421 acagcaccaa ttgccgcgcc tgccaaaagt gccctcaaac ctcctgatac ctggattact
      481 tcatttgctt cctcgattat ctccacatgt aaacccagtg gttttgccag attctcattc
      541 aaactatcag taaattcttt ggataactgt attgccttat taggatttac atctcttgca
      601 tcaattttta ttaaaactgt atgttttttc cttgtaacct tgactttttc cctaacttca
      661 tcaaagctca tattcatcga atctgcagtg gcagcaataa catcgttgct ggcaaaaacc
      721 ccagcaaaag tctcagccat ataatgactg acggacagtt gagagtaact tgaaatagtt
      781 tcttgcgttt tcactgctac tgttgttctt gccacatatt ccttcgctat aactgttttc
      841 tgaacaaccc atgatatcaa agagaaaatg aaacttgata aaacaataat aatgatgtgc
      901 cttgttttaa ttcttttcat cactcaaaaa accgttatcc ttcagttttg ttatgaatga
      961 atcgatcccc tgtctggccg tctgttcgtc aacctcgtat gcagcaagca ttttatccag
     1021 caagtcatca tactcaatac cttcagacag atgcttccac aaaaacgttc cagtagagtt
     1081 cagtcttatc ataccattga aacgctttga ttcctcaccc actgctacta cgacgcttac
     1141 accatctatt tcacgcaaca tatatccatc cttaatcttc aaaactaaaa acccccttaa
     1201 tatagccctg tccagttgtc gtcatcataa tcaggcacat ctgtagctcc gggagtatcg
     1261 gattcggttg gttgtggcgt gctggtactt tgtgacggcg tgctggtact tgtttcaggc
     1321 gtgctggtag cactgcctgg tgtttttgta ttatcaagca gatcattaaa atcaattcct
     1381 tcatcagtcg cttcgggagt tggtgtcgtt tttacatcct ttttgtcatc cccgcaggaa
     1441 acaagcaaac ttattgataa tacaattatg aataatgcta aaaacttttt catagctcct
     1501 cctatatgcc catcctgttc aaatttatac acctttatta taggttccaa acccatgcaa
     1561 gtcaataaaa caattaacaa aaaacaatca gaatgtaaca gaaatacaac aatttatgca
     1621 ccatgatctt ctttcaagtc aatgttttcc aagttcttgc atctgtttgc aatctcaatt
     1681 aattcaagct ctgtctctat ttccacagaa tatgggtagt atcttgtata tctatcaaac
     1741 ttctttctaa tctcttgcgc tttttttagg tccttctccg caagcagtgc atatgcatac
     1801 tcggtacgaa ttacattagg aaattttctc atagccttca taagtttctt ttgccttgca
     1861 tcaagcatgt cgtcaactac attcttacgg ttttcattaa ccagttcaca ataaattcta
     1921 tcgcacacaa gaataccttt gtgaagagcc acaattgaac tttcaatatt catgaaatgt
     1981 gtcataagtt tgtctgcctc gtcaaacttg tgattatcca tcagtcggtt gcaggcaaac
     2041 actccgataa ccgccatcat gctgttcttc atctcttcat cgcttggaat ctcaaaccac
     2101 tcatccggca tgtcttttag cctgacgcca ttggcaagca attcatttat cttcagctgc
     2161 agccaaaagg agcgcctgga ttccttgttt tttgtaaggg tcaaggcatt atatccgtca
     2221 ttgtccatca tgcccatgcg cattggtata ccgtttaata atgcaagcac tattccacta
     2281 gaagcaagaa acataagaaa aagtgaaagt ctgggcatat gtagaacttt aaagtgagaa
     2341 aacagaaaca aaagaccggt cagagtattc aagatggacc ctcccaaatt gtataaaaca
     2401 acagggaatt tctcttcttt catgtcagga ggcgccatca ggacctgtcc gcctgttcct
     2461 gctacagaaa aatgtcttaa cttaatcttt ccagattctt ttatcagcat aagactcatt
     2521 atgcggaaag atgaaaactt gtagccagtc atgaaagcaa atatcaaatg acccagttca
     2581 tgtattacaa tttggatgaa tatagcaaaa tacatcagga taatcatgac aaaaaatgac
     2641 attattcttc cagtaaacga aatttccaac tttccaatat atttcccaat aagccaacca
     2701 aaaaaagctc caa
//
LOCUS       DAONVY010000320         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25522_length_2712_cov_3.206248, whole genome shotgun sequence.
ACCESSION   DAONVY010000320 DAONVY010000000
VERSION     DAONVY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25522_length_2712_cov_3.206248"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLI11_08315"
     CDS             <1..197
                     /locus_tag="PLI11_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52906.1"
                     /translation="GDHIEKQFENVEDGVKEEMRLLLERLPALSENTWNKEKTRTFEE
                     FIVDAESLNERLTPILKIGE"
     gene            complement(241..2079)
                     /locus_tag="PLI11_08320"
     CDS             complement(241..2079)
                     /locus_tag="PLI11_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ52907.1"
                     /translation="MVKDVFYELPAGSVKIDGLFEDKINNIVENTLKKLNYEKLVDFF
                     RLTLNQFATGEFWGKTVRAGCLLYNFTQDEELFSILEETVNDMLSVQKEDGCISTVPY
                     ETQPNGTHGSDMWERKYAMLGLLSYYDITGREDVLNAIMRLCDYTISQVGNPPKTPIT
                     ETGWAFYGIESSSILEPVVRLYNITREKRYLDFAKYIIDSGACKRENIFEAIYKKEPK
                     DIGGNGIPEESIAKAYEMMSCFEGMLEFYRATGDEKYKENVQIFYDKLIEQEITLLGS
                     GGANKPFNLGPGIGEQWNHTAYEQTNPDIDLMMETCVTVYWMKLCHQLFRLTGNSKIA
                     DQLEISAYNALIGAIKPDGKFFEYFPRFNGTRNPKVNYSFNIDGFDLSCCTANGPTGL
                     ALFASGIYMKTENGLAINYYVPGKVTLEDFSLVVDTDYPVTGRVNLKVETKEKKQMEL
                     MFRVPSWTKAFTIRINDEDVTNIDNGYAKVDRLWQSGDLISLDIDMTCKVFDAPLGSN
                     RLGDDFVAVMRGPILLARDRRLGEDIYEKVSFWHLDFEPRKQQKRVREYDSEPECILV
                     EHEIDALMQVKIKTDKGYINMIDYQSAGITWDESSEFASWLPRENR"
     gene            complement(2072..>2712)
                     /locus_tag="PLI11_08325"
     CDS             complement(2072..>2712)
                     /locus_tag="PLI11_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52908.1"
                     /translation="DISVRDAKGIGKNHYINTSGRNDFKVCKVTHDNNNVYFYVETME
                     PITPFDGPYWMNLFIRVKGKGAHDKSWEGFQYILSRRNVREDYTCLERSTGGWNWEIV
                     YDYVEYKVTENKMELAIPRYALGIDNTKNIDIEFKWFDNMQHEGDPLEFYLNGDTAPN
                     QRFAYSYRFIGDNKGDKSPALTIALGAGLVVLSILIVIVILISNKKKRGING"
BASE COUNT          732 a          611 c          492 g          877 t
ORIGIN      
        1 ggggagacca cattgaaaag caatttgaga atgttgaaga cggtgttaaa gaggagatga
       61 ggttgcttct tgaaagactt cctgctctta gcgagaatac ctggaacaag gaaaagaccc
      121 gtacctttga agaatttatt gtggatgcgg aatctttaaa tgagagactt acaccgatac
      181 taaaaattgg tgagtaaata aaatgccaga ggctaaaacc tctggcattt ttcaatactc
      241 ttatctgttt tctctaggca accagcttgc aaactcggag ctttcatccc atgtaatgcc
      301 tgcagactgg taatcaatca tatttatata tcccttgtct gtcttgattt ttacctgcat
      361 aagtgcgtca atctcatgtt ctaccagaat gcattcaggc tcgctgtcgt attcacgcac
      421 cctcttttgc tgctttctcg gttcaaaatc aagatgccag aaagaaactt tttcataaat
      481 gtcttctccg agccttctgt ctcttgcaag aagaatcgga cctctcataa cggctacgaa
      541 atcgtcgcct aatctgttgc taccaagagg agcatcaaac actttgcatg tcatgtcaat
      601 atctaggctt attaaatcgc cactctgcca cagtctgtca acctttgcat atccattatc
      661 aatatttgtt acatcttcat catttatacg aattgtaaaa gcttttgtcc aggaaggtac
      721 tctgaacatt agttccattt gcttcttttc ctttgtctcc accttgagat tcactctgcc
      781 tgtaacagga taatcagtat caacaacaag gctaaagtcc tccagtgtga cttttcctgg
      841 tacataatag tttatggcaa gtccgttttc agtcttcatg tatatgccag aagcaaacag
      901 tgcaagacct gtaggaccgt ttgctgtgca gcagcttaag tcgaacccat caatattaaa
      961 actgtagttg acctttggat tccttgttcc attgaatctg gggaagtact caaagaattt
     1021 cccatccggc tttatagcgc cgattagtgc attgtatgca gaaatttcta gctgatccgc
     1081 aatctttgag ttgcctgtca gtctaaaaag ctgatgacaa agcttcatcc agtaaacagt
     1141 cacacaagtt tccatcataa gatcaatatc tggatttgtc tgctcgtagg cggtatggtt
     1201 ccactgctcg ccaattccag gacctaagtt aaagggcttg ttagctccac cagagcctaa
     1261 cagggtgatt tcctgctcga taagtttgtc ataaaagatt tgtacatttt ccttgtattt
     1321 ttcatcgccg gttgccctgt agaactcaag cataccttca aagcaggaca tcatttcata
     1381 ggcttttgca atgctttctt caggtatgcc attgcctcct atatcctttg gttccttttt
     1441 gtatattgcc tcaaagatat tctccctctt gcatgcccct gaatctatga tatatttggc
     1501 aaaatcaaga tatctctttt cccttgttat attatacaac ctcactacag gctcaagaat
     1561 ggagctggac tctatgccgt aaaacgccca gcctgtttca gtaataggtg tttttggcgg
     1621 attgcctact tgcgagattg tatagtcgca aagcctcatt attgcgttca gaacatcttc
     1681 tctgcctgta atgtcatagt aagaaagaag accaagcatt gcatactttc tctcccacat
     1741 atcagagccg tgagttccgt tgggctgggt ttcataaggt actgtactaa tgcatccgtc
     1801 ctccttttga acagacagca tgtcgtttac cgtttcctcc aggatactaa acagttcctc
     1861 atcctgggtg aaattataca gaagacagcc tgcacgaacc gttttacccc agaattctcc
     1921 tgtggcaaac tgattgagtg taagcctgaa aaagtccacc agtttctcat aattgagttt
     1981 tttcaatgtg ttttccacaa tgttgttgat tttgtcctca aacagcccgt caattttaac
     2041 acttcctgca ggcaattcat agaatacatc tttaaccatt aatacccctc ttctttttat
     2101 ttgatattag tattactatc acaattaaaa tggatagtac aaccagtcct gctcctaaag
     2161 ctattgtcaa agcaggtgat ttatcgcctt tgttatcgcc aataaatctg tatgagtaag
     2221 caaacctctg gtttggagcc gtgtctccgt ttaaatagaa ctccaaaggg tcgccttcat
     2281 gctgcatatt gtcaaaccat ttaaactcta tgtcaatgtt tttagtattg tctataccaa
     2341 gagcataacg cggaattgca agttccatct tgttttctgt taccttgtat tcaacgtagt
     2401 cgtaaacaat ttcccagttc caacctccgg tgcttctttc aagacaggtg tagtcttcac
     2461 gtacatttct tctgctaaga atgtattgga agccttccca ggacttgtca tgagcacctt
     2521 tgcctttaac acgaataaaa agattcatcc agtaaggccc gtcaaaggga gtaataggct
     2581 ccatagtttc cacatagaaa tatacgttat tgttgtcatg ggttactttg cagactttaa
     2641 agtcatttct tccagacgtg tttatataat ggtttttgcc aatacccttt gcatccctga
     2701 ctgaaatgtc at
//
LOCUS       DAONVY010000321         2711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25535_length_2711_cov_2.981928, whole genome shotgun sequence.
ACCESSION   DAONVY010000321 DAONVY010000000
VERSION     DAONVY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25535_length_2711_cov_2.981928"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..290)
                     /locus_tag="PLI11_08330"
     CDS             complement(36..290)
                     /locus_tag="PLI11_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPZ52909.1"
                     /translation="MEIRVCIGSSCYLKGSRDVINELNHLIKENSLNSMVTLKGSFCM
                     GKCSMNGVTVCCDDRFFFVEPDNVDDFFKNVIVPSIPRAI"
     gene            497..1660
                     /locus_tag="PLI11_08335"
     CDS             497..1660
                     /locus_tag="PLI11_08335"
                     /inference="COORDINATES: protein motif:HMM:NF026101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4474 domain-containing protein"
                     /protein_id="HPZ52910.1"
                     /translation="MLYTYFDGTSFYAVILSLNLLQIISLIVIGIVVLIGFALASINV
                     IGLLMPALQKLMRKLGEHIGMTAPGIEPQEVFVSFDDLIKETGFAYDKERDFFYSSLY
                     PWQRRFGYTRLYDVVAPTLSMIIDSEPIRFEYKGYKWMIELWKGQYGMTTGCEVGVYS
                     TKKPINFLSTLSQKERDQELDDEERINRLLELAVPDIGEEEEDKGIRRKISDYIDMDE
                     LLDNTTFYYSADNDDLLYMSFVARKNGEIILAHRDRHWWLTAFKLGEFSEPSEITMSV
                     SITLKDNEMLNAFLEGMYKLGYKPGDVKVHNTTVQFLFDKPRSKQPITRNRITDMIAQ
                     SRNKAFCELYQELTRGATDMNEKLKLLYENNPELYKQALKLGKSKTLFTGLQD"
     gene            1670..2557
                     /locus_tag="PLI11_08340"
     CDS             1670..2557
                     /locus_tag="PLI11_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPZ52911.1"
                     /translation="MGYIKRIDKVFENAKVIEFDDTSKFIFMSDCHRGNGNGADNFAK
                     NQNLFFYALTKYYNEGYTYIELGDGDELWENKDICEIIATYSNIFWLFSEFYKQNRLY
                     FIYGNHDIVKRRKNAKKDMSVFFDECAQQEAPLFPNLCMHEGIVLRHAMTGKKILLIH
                     GHQADLFNYKFWRIGRFLARHLWRPLELIGIQDPTRTSRNSSKRNKVEAKMIEWVKKR
                     NRIMIAGHTHRPVFPDSFDLPYFNDGSCVHSRCITCIEIVEGEISLIKWSYKVKHDGT
                     VYIGKDILEGPEKLCHIWI"
BASE COUNT          866 a          487 c          598 g          760 t
ORIGIN      
        1 cggcaagtat caactgggag catgtaaatt aacaattata tcgctctggg aatggaggga
       61 actattacat ttttgaaaaa gtcgtcaaca ttatctggtt ctacaaagaa aaacctgtca
      121 tcacagcaaa cagtaacacc gttcatactg catttaccca tgcagaagga acctttcaaa
      181 gtgaccatgc tgtttagact gttttctttt attaaatggt tcagctcgtt tattacatct
      241 cttgagcctt tcaggtagca ggagctgcct atgcatactc ttatttccat ataaatccct
      301 cccaaagcga ggttatttgt taaaaattta accttctatc tataatatac gtctttgtta
      361 tttatttgtc aatcttaaat gtaacggaag cgttaaattc caagcaacta tttgtgaaat
      421 tatatttttc ctcaatggct taacaatcaa ctttggttct gaatactatg ttattggaga
      481 aaattatgaa gtgattatgt tgtatacata ttttgatgga actagctttt atgcagtaat
      541 actaagtttg aatttattac agattataag tctgattgtt ataggaatag ttgtacttat
      601 agggtttgcc ctggcctcta ttaatgtgat tggattgctc atgcctgctt tgcaaaagct
      661 tatgaggaaa cttggcgagc acattggcat gactgcacca ggaatagaac ctcaggaggt
      721 gtttgtttcc tttgatgact taatcaagga aacgggcttt gcttatgaca aggagaggga
      781 cttcttttac tcttcccttt acccatggca aagacggttt ggctacacaa gactgtatga
      841 cgtggttgca ccgacactga gcatgatcat tgatagcgaa ccaatcaggt ttgaatacaa
      901 aggttacaaa tggatgatag agctgtggaa aggtcagtac ggaatgacca ccggctgtga
      961 ggttggcgtt tattcaacga aaaaacccat caacttcctc agtacacttt cgcaaaaaga
     1021 aagggatcag gagctggatg atgaagagag gataaatagg cttttggaat tggcagtgcc
     1081 tgatatcggg gaagaagagg aagataaggg cataaggcgg aaaatttcag attatataga
     1141 tatggatgag cttttggaca acacaacctt ctactattcc gccgacaatg acgaccttct
     1201 ttacatgtcc tttgtagcca ggaaaaacgg agaaataatt ctggctcaca gggataggca
     1261 ctggtggctg acagcgttta aactgggtga gttttcagaa ccctcagaaa taaccatgag
     1321 tgtcagcatt acattgaagg ataacgagat gctgaacgct ttccttgagg gaatgtataa
     1381 gctgggatac aaaccagggg atgtcaaagt acataacacc acggtacaat ttctgtttga
     1441 caaaccgcgt tccaagcagc ctattaccag aaaccgcata actgacatga ttgctcagag
     1501 cagaaacaag gctttttgcg aattgtacca agagcttacc cgtggtgcaa ctgacatgaa
     1561 tgaaaagctg aagcttcttt acgagaataa tcctgagctc tacaagcagg cacttaagct
     1621 gggcaagtcc aagacgctct ttactggttt gcaagactag gaggcggcta tgggatacat
     1681 aaagagaatt gacaaagtgt ttgaaaatgc aaaggtaata gagtttgatg acacctcaaa
     1741 gttcattttc atgagtgact gtcacagagg taacggcaat ggcgcggata actttgccaa
     1801 gaaccagaac cttttcttct atgctttgac caaatattac aacgaaggct acacatacat
     1861 agagctggga gatggtgatg aactttggga gaacaaggat atttgcgaaa ttattgcgac
     1921 atacagcaat atattttggc tgttttcaga gttttacaag caaaaccggc tgtactttat
     1981 atacggcaac catgacattg taaaaaggag aaaaaacgca aaaaaagaca tgagtgtttt
     2041 ctttgacgag tgcgctcaac aagaagcgcc tcttttccct aacttgtgca tgcatgaggg
     2101 tatcgtcctc aggcatgcca tgacagggaa aaagatattg ctcatacacg gacatcaggc
     2161 agacttgttc aattacaagt tctggcgaat aggaagattc cttgcaagac atttatggag
     2221 gccacttgaa cttatcggaa tccaggatcc tacaagaacg tcaagaaaca gcagcaaaag
     2281 gaataaagtt gaagcaaaaa tgattgagtg ggttaaaaaa agaaacagaa taatgatagc
     2341 aggccacacc cacagacctg tgtttccgga cagttttgac ttgccttact ttaacgatgg
     2401 gagctgtgtt cattcaagat gcattacctg catagaaatc gttgaaggag aaatttcact
     2461 gataaagtgg agctacaagg taaagcatga cgggaccgtt tacattggaa aggacatttt
     2521 ggagggcccc gaaaagctgt gtcatatatg gatttgattc tatgaggctg acggcctctt
     2581 tttacttgtt ggtagaaaat aatggaataa tgaagggatg taatccactg ttcaacatgt
     2641 gttcttactc tgttaattga cttttacata attaaatatt agaatataat gaactcaatt
     2701 taaaaggggt c
//
LOCUS       DAONVY010000322         2701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25663_length_2701_cov_3.604308, whole genome shotgun sequence.
ACCESSION   DAONVY010000322 DAONVY010000000
VERSION     DAONVY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25663_length_2701_cov_3.604308"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..2204)
                     /locus_tag="PLI11_08345"
     CDS             complement(249..2204)
                     /locus_tag="PLI11_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52912.1"
                     /translation="MRRKMHVENKPKNAFKTLARMYKYMSGHTLGLVFAIILIIFSSL
                     AGIIGTSLLRPLINNLMNNIKLLMDAGHDASTAKEIIMSGVFDTLVIMIVVYFLGALS
                     TYFGNRLLLIISQKTTNRIRKELFDHLQSLPVGFFDRNTHGELMSRFTNDMEHITMAL
                     EQSLSQTITSAITIVGTFIMMVYYSPFLTIFVILMLFVMLRVVKIVGKKSAVNFRNQQ
                     KSLGELNGFIEEMMEGQKVVKIFNHEEKNQSEFEALNEELRKASTDAQTYAGILMPIM
                     GNLSYVQYAITACVGALLIANPVNLAFFAMDVGTLASFLQFTRAFSQPITQIANQTNT
                     LLAAIAGAERIFSVLDEEPEEDNGKTTLAVAKRNENNELVHVDKPFVDMTQCQKENLI
                     PVWKTPTPDGYKLTELKGDVRFNNVVFGYVEDVIVLKNLSLYAKPGQKIAFVGSTGAG
                     KTTVTNLLNRFYEIKEGTITYDGIDIRDIKKADLRRTLGFVLQDVHLFEGTIKDNIRY
                     GRLDASDEEVVQSAKIANAHSFIKRLPQGYDTILTKDGINLSQGQKQLVSIARAAISD
                     PPVLVLDEATSSIDTRTEKLIEKGMDTLMKDRTTFVIAHRLSTVRNANAIMVIENGEI
                     IERGDHEDLLDLKGRYYNLYTGMSELS"
     gene            complement(2197..>2701)
                     /locus_tag="PLI11_08350"
     CDS             complement(2197..>2701)
                     /locus_tag="PLI11_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ52913.1"
                     /translation="AMILQKNTLFSGTIRDNMKWGNEKATDEEIIEALKQAQAWPFVS
                     KLEKGLDHWVEQGGANFSGGQKQRLCIARSLLKKPKVIILDDSTSAVDMATDAKIRAS
                     FKENLKGITVIIIAQRVRSISHCDRIIVMDDGKINAIGTHEELLENCEIYEDIYKSQQ
                     EGVLADA"
BASE COUNT          682 a          599 c          534 g          886 t
ORIGIN      
        1 tgcttgtgta ttctttcaag tcggatacgg aaaaatccat tttgtagaca ttctctacgg
       61 attcgtatgc tttagtgtcc cttggttcaa atcccgactc gccctgagcg ggtacaagta
      121 taccaaggca aatgactaag caaatgatta ttttaagtat tttccacata tcacacctca
      181 tcaaattatt gttgataaat atattataat gattaataga aaatttcaat gtttaattca
      241 aattaacatt acgatagctc gctcatgccg gtatacaggt tgtagtatct gccttttagg
      301 tcaagcaaat cctcatggtc tccacgctct ataatttcac cgttttcaat aaccatgatg
      361 gcattcgcat ttctcacagt agacagcctg tgggcaatta caaaagtggt cctgtctttc
      421 atcaatgtat ccattccttt ttctatgagc ttctccgtcc tagtatcgat ggagcttgtt
      481 gcttcgtcaa gtacaagcac cggagggtct gatatggctg ctcttgcaat ggatacaagc
      541 tgcttttgac cctgggacag atttatgccg tctttggtca atatggtgtc atagccttgt
      601 ggaagccttt ttataaagga gtgggcattt gcaatctttg cgctctggac aacttcttcg
      661 tcgctggcat ccagccttcc gtacctgata ttgtccttta tggttccctc aaaaagatgc
      721 acatcctgca gaacgaaacc caatgtcctt ctaaggtccg ccttctttat atcccttata
      781 tctattccgt catacgttat agttccttcc tttatttcat aaaaacggtt caaaaggttc
      841 gtgactgtgg tttttccagc acccgttgat ccgacaaaag caatcttctg accgggcttt
      901 gcgtaaagtg aaagattctt tagaacaata acatcctcca catatccaaa cacaacatta
      961 ttgaatctta catctccttt tagctcggta agcttgtagc catcaggagt cggagttttc
     1021 catacaggaa ttagattctc cttctggcac tgggtcatgt caacaaaggg tttgtcgaca
     1081 tgaacaagct cgttgttctc attccttttt gcaaccgcca gtgtggtttt tccattgtcc
     1141 tcctccggct cctcatcaag aacagaaaag attctttctg cacctgcaat cgctgcaaga
     1201 agagtgttgg tctggtttgc aatctgtgta atgggctgtg aaaatgctct tgtaaactgg
     1261 agaaaagaag ccagcgttcc cacgtccatg gcaaagaagg caagatttac agggtttgca
     1321 ataaggagag cacctacaca tgcagtaatt gcatactgta cataagacag atttcccatg
     1381 atgggcataa gtattcctgc atatgtctgg gcatctgtag atgcctttct gagttcttca
     1441 ttaagcgctt caaattcgct ctggtttttc tcttcgtgat tgaatatctt tacgactttc
     1501 tgtccttcca tcatttcttc tatgaaaccg ttcagctcac caagactttt ctgctggttt
     1561 ctaaagttaa ctgcgctctt tttacctaca attttgacaa ccctgagcat tacaaaaagc
     1621 ataaggatta cgaatattgt aaggaagggg ctgtagtaga ccatcataat gaaggttcct
     1681 acaattgtaa ttgcagatgt aattgtctgg gacaggcttt gctcaagagc catggtgatg
     1741 tgttccatgt cgtttgtgaa acggctcatt agttctccgt gtgtgtttct gtcaaagaaa
     1801 cctacgggca gactttgcaa atgatcgaac agttctttcc ttattctgtt ggtagtcttt
     1861 tgcgatatga taaggagcag tctgttgcca aaatatgtgg acaatgctcc aagaaaatag
     1921 acaactatca taatgacaag ggtgtcaaac actcccgaca tgatgatttc ttttgctgta
     1981 cttgcatcat ggcctgcatc catcagcagc tttatattat tcatcaggtt gtttatgagg
     2041 ggtctcagca gtgaagtgcc gataattcct gccaaggagc tgaaaatgat caagattatt
     2101 gcaaacacaa gccctaatgt gtgcccggac atgtatttgt acatgcgcgc aagggtttta
     2161 aaagcgtttt taggtttatt ctctacatgc atttttctac gcatctgcaa gcactccttc
     2221 ctgctgtgat ttgtatatgt cttcatatat ctcgcaattt tcaagaagtt cctcatgggt
     2281 tccgattgca tttatttttc catcatccat gactattatc ctgtcgcagt gggatatgga
     2341 tctgactctc tgtgcaatta ttatcacagt aattcctttc aggttctctt tgaaagaagc
     2401 acggattttc gcatccgttg ccatgtccac ggcacttgtg gagtcgtcca ggataataac
     2461 ctttggtttt ttcaacaagg aacgggcaat acagagtctt tgcttctgtc ctcctgagaa
     2521 gtttgcaccg ccctgttcaa cccagtggtc aagacctttt tccagcttgc ttacaaaagg
     2581 ccaggcctgg gcttgtttta aagcctcgat gatttcttca tccgtggctt tctcatttcc
     2641 ccatttcatg ttgtctctta tggttcctga gaaaagggtg ttcttctgca gaatcatagc
     2701 c
//
LOCUS       DAONVY010000323         2698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25708_length_2698_cov_3.297768, whole genome shotgun sequence.
ACCESSION   DAONVY010000323 DAONVY010000000
VERSION     DAONVY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25708_length_2698_cov_3.297768"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLI11_08355"
     CDS             <1..637
                     /locus_tag="PLI11_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPZ52914.1"
                     /translation="DKYWIWARKKVERLMPGVFCGEPLDMLSVSKLLKDYCDQFIPRI
                     EVFEEGADFFRDMQKAGKEVWVYSCCFPEEHWYLNKFIDHPHIHSRLITWACYTQNIT
                     GFLHWGYNYFAGNDLYSCLPNARFKGDGHIVYPNVEYNTINISTRFIATRDGIQDYEL
                     LKIAERKFKDATFALSRKIARSFSEFNNNPESVDNTVSRLLKLAEVSQNMV"
     gene            660..944
                     /locus_tag="PLI11_08360"
     CDS             660..944
                     /locus_tag="PLI11_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HPZ52915.1"
                     /translation="MKLVFIVYYAILNIVGFLVMLSDKRKAMYHRTRTKESTLFLVSI
                     LGGALGVFLAMHIFRHKTKHGSFIYGIPAILIVHILLISYIAYNFPNLFK"
     gene            963..1835
                     /gene="ylqF"
                     /locus_tag="PLI11_08365"
     CDS             963..1835
                     /gene="ylqF"
                     /locus_tag="PLI11_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HPZ52916.1"
                     /translation="MVIQWFPGHMARARRVLKEGLAMVDVVVEMVDARIPISSRNPEI
                     DKIIGNKPRIIACNKADLADAEMNKYYKRYFNEKGIKVIFTDSKTGNGVRNVLKEIEN
                     VMSEKMERQKAKGRKAMIVKAMIVGVPNVGKSSFINRITKKYSAVTGDRPGVTRNKQW
                     LKANDNVYLLDTPGLLWPKLEDQDAAKKLAITAAIKDDILDTSLLAVYLLELLRDKYP
                     NAIQERYKFDPKDMTGVQMLEECGKRRGCLVHGGEVDYQRASTVVLDDFRNGRLGKIT
                     LDHEVEEAGEETSD"
     gene            1828..2448
                     /locus_tag="PLI11_08370"
     CDS             1828..2448
                     /locus_tag="PLI11_08370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPZ52917.1"
                     /translation="MTEEINMLEYENKLKEEGIYIVGGIDEAGRGPLAGPVVAACVVF
                     DTRNPLPEARDSKKLTEKKREELFDKIIESCLAYGIGVVDNDVIDEINILNATYLAMQ
                     KAVLDLNEKNPEVVLVDHVHIPDMNIKQISITHGDASSLCIAASSIIAKVTRDRIMRE
                     LDSLYPGYGFARHKGYGTKAHYEAIETLGISKVHRKTFLKKYITTS"
     gene            complement(2438..>2698)
                     /locus_tag="PLI11_08375"
     CDS             complement(2438..>2698)
                     /locus_tag="PLI11_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52918.1"
                     /translation="KDFSPEDGTDLLIFEAPEPYGPFSLVHVEEYWEGKNFTPYCPRL
                     PLKWVEPDGVTCWLQFSGSWGVEGQKNGYYRSNVRRMRLIMK"
BASE COUNT          894 a          485 c          613 g          706 t
ORIGIN      
        1 ggacaaatat tggatatggg caagaaaaaa ggttgaaaga ttaatgcccg gcgttttttg
       61 cggagagcct ttggatatgt tgtcagtctc aaaactgtta aaagactatt gtgatcagtt
      121 catacccaga atagaagttt ttgaggaagg agcggacttc ttcagagaca tgcaaaaagc
      181 tggcaaggaa gtctgggtgt attcatgctg cttccctgaa gaacattggt accttaacaa
      241 gttcatagat cacccccata tacattccag actgataacc tgggcatgtt atactcaaaa
      301 cattacagga ttcctgcatt ggggctacaa ctattttgca ggtaatgacc tttacagctg
      361 tcttccaaat gccaggttca agggtgacgg ccatattgtt tatcccaatg tggaatacaa
      421 tacaataaat atatccacaa ggttcattgc aacaagggac ggcatccagg attatgagct
      481 attaaaaatt gctgaaagaa aattcaagga tgcaactttt gctctgtcca gaaagatagc
      541 tagaagcttt tcggagttta ataataatcc cgagagcgtt gacaatactg tttcaagact
      601 tctgaaactt gcagaagtat ctcagaacat ggtatgattg tgatatgggc ggattaactt
      661 tgaagctagt atttattgtg tattatgcca tattgaatat tgtgggattc cttgtaatgc
      721 tctcggacaa aaggaaggcc atgtaccaca gaacccgtac caaggaatcc actcttttcc
      781 tagtatccat actaggagga gctttaggag tgtttcttgc catgcatata ttcagacata
      841 aaacaaagca tggcagtttc atttatggaa tacctgcgat attaattgtt cacattcttt
      901 tgatatcata tatagcgtac aattttccaa atctatttaa gtaatgaaag gtggaaattg
      961 aaatggttat acaatggttt ccgggccata tggcaagggc cagacgagta ttaaaagaag
     1021 ggcttgccat ggtggatgtt gttgtagaaa tggttgatgc aagaatacct ataagcagca
     1081 gaaacccgga aatagacaag ataataggga acaagcctag aatcatagcc tgcaacaagg
     1141 cggacttggc agatgcagaa atgaataagt attataagag atattttaac gaaaaaggca
     1201 tcaaagtaat ctttaccgac tcaaaaacag gaaacggtgt aaggaatgtt ttaaaggaaa
     1261 tagagaatgt aatgtcagaa aagatggaac gacaaaaggc caagggacgc aaagccatga
     1321 ttgttaaggc tatgatagtt ggagttccaa atgtgggcaa atcctcattt ataaatcgca
     1381 ttacaaaaaa atactctgca gttacaggcg acagacccgg tgttacaaga aacaagcagt
     1441 ggctgaaggc aaatgacaat gtttacctcc tggatactcc aggtcttttg tggccaaaac
     1501 ttgaagatca ggatgcagca aaaaaacttg caataaccgc tgccataaag gatgacatcc
     1561 tggatacctc gcttcttgcg gtttacctcc ttgagctttt aagggataaa tatcctaacg
     1621 caattcagga aaggtacaaa tttgatccga aagacatgac aggagtacaa atgcttgagg
     1681 agtgcggcaa aagaagagga tgtcttgttc acggaggaga agttgattac caaagggctt
     1741 ccacagtggt acttgatgac tttagaaacg gaaggcttgg aaaaattact cttgatcatg
     1801 aagtggaaga agcaggggag gaaacaagtg actgaagaga taaacatgct tgagtatgaa
     1861 aacaaactga aagaagaagg aatatatatt gtaggtggaa ttgacgaagc aggacgcggt
     1921 cctcttgccg ggcctgttgt tgcagcatgc gttgtttttg atacaaggaa tcctttgcct
     1981 gaagcaaggg attccaagaa actaacagaa aagaaaagag aggaactttt cgataagata
     2041 attgaaagct gccttgccta cggtataggt gttgtggaca atgatgtgat agatgaaatc
     2101 aacattctta atgccacata ccttgcaatg caaaaggcag tgttggattt aaatgaaaaa
     2161 aacccagaag tggttctggt tgatcatgta catattcctg atatgaatat aaaacagata
     2221 tcgataaccc acggcgatgc gtcaagtctc tgcatagcag cttcctcaat tatagccaaa
     2281 gtaaccaggg acagaataat gagggagctg gattcactgt accctggtta cggctttgca
     2341 agacataaag gttatggtac gaaagcccat tacgaggcta ttgagacatt aggcatctcc
     2401 aaggtacata gaaagacttt tctgaaaaaa tatattacta cttcataatc agtctcattc
     2461 tccgtacatt ggacctgtaa tatccatttt tctgaccttc gacaccccag cttcctgaga
     2521 actgaagcca gcaggttaca ccatccggtt cgacccactt gagaggtagt ctggggcaat
     2581 acggagtaaa gttttttcct tcccaatatt cctctacatg cacaagggaa aagggtccgt
     2641 atggttcggg agcttcgaaa attaacaggt cggtaccatc ttcaggcgag aagtcctt
//
LOCUS       DAONVY010000324         2691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25778_length_2691_cov_2.779590, whole genome shotgun sequence.
ACCESSION   DAONVY010000324 DAONVY010000000
VERSION     DAONVY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25778_length_2691_cov_2.779590"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="PLI11_08380"
     CDS             complement(<1..1788)
                     /locus_tag="PLI11_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52919.1"
                     /translation="MSFRKFTTLLSCLLLPLSITVGCTNVLKKTVDAYSVNEGRNFNY
                     LCGVDQFGRIFEPVTGMNEEKHVGMFYFLWHGESGVTTHNITQLLKEDPDALFDVKGS
                     SKSPLNAFHYWDEPIFGYYRSDDKWVIARHCELLSQAGIDFLVFDTTNAFIYEKSYTA
                     LLEVFSEYQERGWNVPKIAFYTNSMSIRTMNQLYEKLYSKGLYKELWYCPDGERPLII
                     GALNAEHDKKIYNNPSYNPPELTEEFLNFFDLRSTQWPFDPFIPDGFPWMEWLYPQPN
                     HNGIMNVSLAQHPQLPFSNSYFNRNLNKGRGYDFDKKENIESEYAKGLNAQYQWNTVH
                     KNAKDVHTVFVTGWNEWIAQKLNLSGAIGFVDCFNEEFSRDIEPSKNGTYQDAFIIQL
                     IQNVRKFKGLNEKFKAPDKATIDIKGDVSQWDKIRNTYINISETETGRDSNSVDFKYK
                     YVQEKPRNIIKEVKITHDKNNAYIMIKCVDDIISDTDENWMNVFIGTNDVKLQGWECY
                     NYVIGRSVDGDKIALNTLDENGSATKVADARFSIKGDVMQIEVKLKDLGLKTKDKGFY
                     FKVADGVENYEDIMDYYVTGESFPMGRLSY"
     gene            complement(1778..>2691)
                     /locus_tag="PLI11_08385"
     CDS             complement(1778..>2691)
                     /locus_tag="PLI11_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52920.1"
                     /translation="FIFVTGWNEWVALRFSEWQGVENAFPDQFNDEYSRDIEPSKGDL
                     KDHYYYQLVENIRRFKGVSKPEKLNAKKTIDIFGSLDQWDEITPFYHYTQNTWDRDHN
                     GYKGTHYTNKTMRNDIRVAKVSFDRKNIYFYVETVDKLTSHKDRGWMRLFIDTRKATK
                     NSKDWEEIEYVVNRINPTDQVAYVEKSKGGWNWEVVAEVKYSVKDNVLQLEIPRKVLG
                     LNSLGDPVFNFKWADNNCLNGDIFTFYTDGDAAPGGRFMFHFNTNQGLSTIATAIITI
                     SVLAVAIVSTLLTTRKKRKERSHKSEF"
BASE COUNT          673 a          577 c          504 g          937 t
ORIGIN      
        1 atagcttaga cggcccattg ggaagctttc gcctgttaca tagtagtcca taatgtcttc
       61 atagttctca acgccatctg ctaccttgaa gtaaaagcct ttgtcttttg tcttaagacc
      121 aagatcctta agttttactt caatctgcat gacatcgccc tttattgaaa accttgcatc
      181 agcaaccttg gttgcgctgc cgttttcatc aagtgtgtta agggcgattt tgtctccatc
      241 aacacttctt ccaataacat agttgtagca ttcccaaccc tgcagtttca catcattggt
      301 gccaataaag acattcatcc agttttcgtc cgtatcactt attatatcgt ctacgcattt
      361 tatcatgatg tatgcattat tcttgtcgtg ggtgattttc acctctttta taatattcct
      421 tggtttttcc tgtacatact tgtacttgaa gtcaactgaa ttactgtctc ttcctgtttc
      481 tgtctcagaa atattaatat acgtattcct tattttatcc cactgggata catcgccttt
      541 aatatctatg gttgccttgt caggagcttt gaatttctca ttcaaccctt taaacttgcg
      601 tacgttctga ataagttgga tgataaatgc atcctgatac gttccgttct ttgaaggttc
      661 tatatctctt gaaaactctt cattgaagca gtcgacaaaa ccaatagctc cgctcaaatt
      721 aagcttttga gcaatccatt cgttccaccc ggttacaaat actgtatgaa cgtcttttgc
      781 gttcttgtgc actgtattcc actgatactg ggcatttaat ccttttgcat attcggattc
      841 aatgttttcc tttttatcaa agtcgtatcc tcttcccttg ttaaggtttc tgttgaagta
      901 ggagttgctg aagggaagtt gcggatgctg ggcaagagaa acattcataa taccgttgtg
      961 atttggttgt ggatacagcc actccatcca ggggaatccg tcaggtatga atgggtcaaa
     1021 gggccactgc gtacttctta aatcaaagaa gtttaaaaac tcttctgtca gctctggtgg
     1081 gttgtaactt ggattgttat atatcttctt gtcatgttct gcatttaatg cgccaatgat
     1141 taggggtctt tcaccgtccg gacagtacca aagttctttg taaaggcctt tgctgtagag
     1201 tttttcgtac aattggttca tggtcctaat ggacatggaa ttggtgtaaa atgcgatttt
     1261 gggtacattc cagcctcttt cctggtattc tgagaatact tccagaaggg cagtgtagga
     1321 tttctcgtaa ataaaggcgt tggtggtgtc gaacacaaga aaatcaatac ctgcttgtga
     1381 aagaagctcg cagtgtcttg caattaccca tttgtcatcc gaacggtaat agccaaatat
     1441 gggttcgtcc cagtagtgga aagcattcaa tgggcttttg ctcgagcctt ttacatcaaa
     1501 caaagcatcc ggatcttctt taagaagctg agtaatatta tgcgttgtga ctcctgattc
     1561 gccatgccat aaaaaataga acatgcctac atgtttttct tcattcatgc ctgtaacagg
     1621 ttcaaaaatc ctcccgaatt ggtctactcc gcataagtag ttaaagtttc tgccttcatt
     1681 tacactatag gcatccacgg tttttttcaa tacatttgta catcctaccg ttatgcttaa
     1741 agggagtaaa aggcatgata acaatgtggt aaatttccta aaactcactt ttatgactcc
     1801 tttcttttct tttttttcta gttgtgagca gtgttgaaac aattgcaaca gcaagaactg
     1861 atatggtaat tattgctgtg gcaattgtac ttaatccctg gtttgtattg aagtggaaca
     1921 tgaaccttcc accgggagcg gcatctccgt ctgtgtaaaa ggtaaagatg tccccattca
     1981 gacagttgtt atccgcccac ttgaagttaa aaacaggatc ccctaaagaa ttaagaccaa
     2041 gaacttttct aggtatctct agttgcagta cattatcctt tacagagtat ttaacttctg
     2101 caacgacctc ccaattccaa ccgcctttgc ttttttcaac atacgccact tggtctgtcg
     2161 ggttgattct gttaaccaca tactcaatct cttcccagtc ttttgagttt ttcgttgcct
     2221 tcctggtgtc gataaaaagc ctcatccagc cgcggtcctt atgggaagta agtttgtcga
     2281 cggtttccac gtaaaagtat atgttttttc tatcaaaact tacttttgcc acccttatgt
     2341 cattcctcat cgttttgttt gtgtaatgtg tgcctttata tccattgtgg tctctgtccc
     2401 atgtgttttg ggtgtagtgg tagaatggag ttatttcgtc ccactggtca agagagccga
     2461 atatgtctat cgttttcttt gcgttaagct tttcaggctt gcttacacct ttgaatctac
     2521 gtatgttttc caccagttga taatagtagt ggtctttcaa gtctcctttt gatggctcta
     2581 tatctcttga atactcatcg ttaaactgat ccgggaatgc gttttcaaca ccctgccact
     2641 ctgaaaatct taaagcaacc cattcattcc agccagtgac gaatatgaat t
//
LOCUS       DAONVY010000325         2686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25826_length_2686_cov_3.885595, whole genome shotgun sequence.
ACCESSION   DAONVY010000325 DAONVY010000000
VERSION     DAONVY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25826_length_2686_cov_3.885595"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLI11_08390"
     CDS             complement(<1..757)
                     /locus_tag="PLI11_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52921.1"
                     /translation="MNRKLFAFLLTLLLVMALVFTACDVKDKSPKETPEPTDTATKAP
                     TPEPTPTPTPKPTVEPISEEKKGVPQEDAEVLFKDTFDEDNLEQYLLKQGEFMIVEDG
                     RLLIPENWSAYIPDVTYDLGVDHSQYEMSVKFKAEEGWDSNPWASLFIGCRGDATTPI
                     ATSNGYFWVAFNFTNKAFVYPGGGGQYEGDAWNLRYFEIELPEDFTEDHTVTVVDCGD
                     VIAYYMNTEEEDYYLILKATFEGDEIVIFGNDGS"
     gene            1016..1660
                     /gene="sleB"
                     /locus_tag="PLI11_08395"
     CDS             1016..1660
                     /gene="sleB"
                     /locus_tag="PLI11_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HPZ52922.1"
                     /translation="MANYETVFDHAKNEEDIDVLSRRGSRGQEVISIQRKLKRWGYYK
                     GNVDGIYGPQTESAVRYFQRKNGLRVDGIAGPQTLRAMGIFSSASSNQGSYQQNVELL
                     ARAIYGEARGEPYTGKVAVAAVIINRTKDSRFPKTIAGVIYQPGAFDAVADGQINLQP
                     DQSSYNAARDALNGWDPTYGCLYYYNPQTATNKWIRSLPIVVTIGKHVFCKSNT"
     gene            complement(1662..2519)
                     /locus_tag="PLI11_08400"
     CDS             complement(1662..2519)
                     /locus_tag="PLI11_08400"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HPZ52923.1"
                     /translation="MSRKRRFVIAAALCAAAVFLFFIFVKFHAPKQEKYINKSGNTIS
                     TRFKVPEGYERVKTEEGSFEYYLQNLPLKKYGSKAYHYNGKINRNAPVYGVFDNEITP
                     KNLEQCADAVMRLWAEYLYYRGEYDRIKFNFVNGFECDYVSYAEGNRLHVDDKICYWY
                     KDAEEDYSYETFRRYMDLVHNYANTTSLQKQAEPVSYEDIRIGDIFVMTASQMNKSLG
                     HAVIVVDMCVNKETGEKLLLIAESTTPASETYVVTDREGNVWIRLDEFGALHTSTWSC
                     PGEFLRRLK"
BASE COUNT          797 a          629 c          497 g          763 t
ORIGIN      
        1 cactgccatc attgccaaaa atgacaattt catcaccctc gaaggtagcc tttagtatca
       61 gatagtagtc ctcttcttca gtgttcatgt agtaggcaat aacatctccg caatcaacca
      121 ccgtaaccgt gtgatcctct gtaaaatctt caggaagttc gatttcgaaa tatctaagat
      181 tccaggcatc tccttcatac tggccgcctc cccctggata aacaaatgcc ttgttagtaa
      241 agttgaatgc aacccagaaa taaccgttgc tggttgcaat gggtgtggta gcatcaccac
      301 ggcacccaat aaacaaagat gcccagggat ttgaatccca tccttcttcc gccttgaatt
      361 taaccgacat ctcatattgg ctgtggtcaa ctcccaagtc atatgtaaca tctggtatgt
      421 aagcggacca gttttcaggt atgagaagcc ttccatcttc aacaatcatg aactcacctt
      481 gttttaacag atactgttcc aagttgtcct catcaaaagt atccttgaat aatacttctg
      541 cgtcttcctg tggcacgcct tttttctctt cggaaatagg ttccaccgta ggtttgggtg
      601 taggggttgg agttggttcc ggtgttggcg ccttggttgc agtatcggta ggttccggtg
      661 tttctttcgg actcttgtct tttacgtcgc atgctgtaaa aacaagagcc attaccaaca
      721 aaagcgttaa aagaaaagca aataattttc gattcataac tcgacctccg tttattctat
      781 atgtgtaaat tatacctatt tcatgaataa attgcaacca cataattaaa caacaatata
      841 tttccatatc atccaatgca ttaatttcta ttagatgata aatactaaca aaaaagaaaa
      901 agaggtgttg tcattgtcca ggaaaacact gaaactactg aaccttttat tcgttttgtc
      961 gtttacaata ggttttttgg gaacaataat atattttaaa aaagaaggca aagatattgc
     1021 aaactatgaa actgtcttcg accatgcaaa aaacgaagaa gatattgatg tcctatcaag
     1081 aagaggttca agaggacagg aagtcatatc tatacagaga aaacttaaga gatggggata
     1141 ttacaaagga aacgtagatg gaatttacgg accccagact gaaagcgccg tccgatattt
     1201 tcaaagaaag aacggcttga gagtagacgg tatcgcagga ccacaaacct tgcgtgccat
     1261 gggaatattc tcttcagctt cttcaaatca aggttcatac cagcagaatg tagagcttct
     1321 ggcaagagca atttacggag aagcacgagg cgaaccttat acaggcaaag ttgccgttgc
     1381 tgcagttatc atcaacagaa ccaaagacag ccgcttcccc aaaacaatag caggcgtcat
     1441 ttaccagcct ggtgcctttg acgctgtagc agacggacag ataaacctgc agccggatca
     1501 aagctcatac aatgcagcaa gagatgcctt gaacggctgg gaccccacct acggttgcct
     1561 ttactactac aatcctcaaa cagcaaccaa caagtggata agatcacttc caattgttgt
     1621 taccattgga aaacacgttt tctgcaagag caacacttaa attatttcag tcgtcttaga
     1681 aactcccccg gacagctcca tgtggacgtg tggagagcgc caaattcatc aagcctaatc
     1741 cacacattgc cttccctgtc tgttaccaca taagtttcgg aagcgggagt agtgctttcc
     1801 gcaattaaca agagcttttc ccccgtttct ttattgacgc acatgtccac cacgatgact
     1861 gcatgcccaa gacttttatt catctgggat gcagtcatta caaatatatc gccaatacga
     1921 atatcctcat aagaaactgg ttccgcctgc ttttgaagcg atgtggtgtt tgcgtagtta
     1981 tgcactaaat ccatgtaacg cctgaaggtt tcataggaat agtcttcttc agcatccttg
     2041 taccagtagc atatcttgtc atccacatgc agcctgttgc cttctgcata gcttacataa
     2101 tcgcactcaa agccgttgac aaaattgaat tttatcctgt catactctcc cctgtagtac
     2161 aagtattccg cccaaagacg catcacggca tccgcacact gctccagatt tttaggagtt
     2221 atctcattgt caaaaacacc atatacaggt gcattcctgt ttattttccc attgtagtga
     2281 tatgccttac ttccatattt cttcaaaggt aaattctgaa ggtaatattc aaaagagcct
     2341 tcctcggtct taaccctttc atacccttca ggcaccttga acctggtact tatcgtatta
     2401 ccggatttgt ttatgtactt ctcctgcttt ggagcatgaa atttgacaaa gataaagaat
     2461 aaaaaaactg ccgcagcaca taaggctgca gcaattacaa atcttctctt gcgactcatc
     2521 caaacctcct gataaactgg gcaggacata cccactcatt cttaacaaac tcaaaattat
     2581 tgtttagttc gtgccatgca cccatcattt cgtcatcatt aagaacaata tacgtctcgg
     2641 ttgcaggcat gtttccttcg gccacaagaa atatcttctc tccggt
//
LOCUS       DAONVY010000326         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25836_length_2685_cov_3.617490, whole genome shotgun sequence.
ACCESSION   DAONVY010000326 DAONVY010000000
VERSION     DAONVY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25836_length_2685_cov_3.617490"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLI11_08405"
     CDS             complement(<1..601)
                     /locus_tag="PLI11_08405"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPZ52924.1"
                     /translation="MDYRNVKKRLVFSTSNNPWFNLALEEYLFNTMEDDTVIMYLRQN
                     ENTVVIGKTQNAWKECRTALLEEEGGYLARRSTGGGAVYHDLGNMCYTFMASSNLYDL
                     ERQLKVILGAVRDQGIEAQFTGRNDITLMDGRKFSGNAFKFIKDKGLMHGTLLLNTDS
                     SVMARYLQVSKAKMQAKGVESVRSRVANLIELNPDITPEK"
     gene            747..2111
                     /locus_tag="PLI11_08410"
     CDS             747..2111
                     /locus_tag="PLI11_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPZ52925.1"
                     /translation="MIGIVSGMVMQRNRHNVAEIIIYPEKEIKNAKYFKEDGLFKELD
                     LHKADNGAYILGGIPTGGPYSVNIDGKTYTDIYVGDVWLLGGQSNMEGVGELTSEDIK
                     MKAEDHIRAFYMDDEWGAAELIRMHDLWNAKDKVHRKLLPDVPPPAGPRVRCVGPGLF
                     FAQKMYDYEQVPQGVICCAHGGTSMDQWSPEGRDLGGDESLYGAMYRRFVHNGSRVKG
                     MFWYQGCSDASPELSEVFEEKMINFVSCVRRDFGENLPFVQVQISRLAAVGDENADKS
                     WTKIREVQRTLHEKIKGLDTVYTISYELSDPIHVSSNSQKVIGRNAAELMYGLIHGYD
                     AKSCLPGIRLDSIKMKMNSHEPLWTDIVVTFSNLHGKLKAEPSAMGFELVSGGTKLGD
                     FIYNVSLIEETAIVRVNMSIDFILQNDVHIAYGYGLNPRCNITDEEGYSIPAFGPVKL
                     SNDE"
     gene            2163..>2685
                     /locus_tag="PLI11_08415"
     CDS             2163..>2685
                     /locus_tag="PLI11_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52926.1"
                     /translation="MTTRERMYRILNHKEADRVPIVDQPWAGTIRRWIREGMPDNVDY
                     RDYFNIDKVERIGVDISPRYPVKLVEETDRYVIYTTGWGVTLKQFKEEDSTPDFIDFK
                     VNSSAAWQDAKKRMLDINEGKVDFAYLEANYPRWVENGSFIQADFWFGFDVAHSWMSG
                     TETILVAMLEEPEW"
BASE COUNT          753 a          498 c          670 g          764 t
ORIGIN      
        1 tcttttcagg agtaatatcg gggttcaatt ctataaggtt tgcaaccctg gaacggactg
       61 attccactcc ttttgcctgc atttttgctt tcgacacctg cagatatctt gccattacag
      121 acgaatctgt attgagaagc aacgtgccgt gcatgagtcc cttgtccttt atgaacttga
      181 atgcattacc ggagaacttt cttccatcca ttaaagttat atcgtttcta ccggtgaact
      241 gggcttcaat accttggtcc ctaacagcac caagaatcac tttgagctgc ctttcaagat
      301 cgtacaaatt ggaagacgcc atgaatgtat aacacatgtt gccgaggtca tggtaaaccg
      361 caccgccgcc tgtgctgcgt cttgcgaggt atccgccttc ctcttcaaga agggcggttc
      421 tgcattcctt ccatgcattc tgtgtttttc caataactac tgtgttttca ttctgccgca
      481 agtacataat aactgtgtcg tcttccatgg tgttgaaaag atactcctca agagccagat
      541 tgaaccatgg gttgtttgat gtagagaata caagtctttt ttttacattt ctatagtcca
      601 tatgtttcat tccttcttga tatttatttg cgaatatctc caattatatc ataaaaaata
      661 tatttgttga attatcttta tgttgacaag ccaattgttt tagggttaat atattgctga
      721 atcttaatgc aaaggagaat tacatcatga ttggaatagt atccggtatg gttatgcaga
      781 gaaatcgtca taatgtggcg gaaataataa tctacccgga aaaagagatc aaaaacgcaa
      841 aatatttcaa ggaagatggc ttattcaaag agttggattt gcataaggct gataatggcg
      901 catatatttt aggcggcatt cccaccggcg gtccgtactc ggtaaatatt gacggaaaga
      961 cttatacgga catttatgtg ggagatgtat ggcttttggg tggccagtcc aacatggaag
     1021 gtgttggtga actcaccagc gaggatataa aaatgaaggc ggaggatcat atacgggcat
     1081 tctacatgga tgatgagtgg ggtgcggcag aattaatacg tatgcatgat ttatggaatg
     1141 caaaggacaa ggtacataga aaactgcttc ctgatgtgcc gcctccagca ggtcccagag
     1201 tcagatgtgt tggtcctggt ttgttttttg cccagaagat gtatgactac gaacaggttc
     1261 ctcagggcgt tatttgctgt gcccatggtg gaacaagcat ggaccagtgg tcgccggaag
     1321 gaagggacct gggcggagat gaaagtcttt acggcgcgat gtatagaagg tttgttcata
     1381 atggttccag ggtaaagggt atgttctggt accagggctg ctccgatgcg tctcctgagc
     1441 tttcggaagt ttttgaagag aagatgataa actttgtaag ctgcgtaaga agggactttg
     1501 gtgagaatct gcctttcgtt caggtacaga tttcaagact tgcagctgtt ggagatgaaa
     1561 atgccgacaa aagctggacc aagataaggg aagtgcagag gacactgcat gaaaagatca
     1621 aggggctgga tacagtgtat accatatctt atgagctttc tgatcctatt catgtgtcaa
     1681 gcaactctca gaaggttatt ggaagaaatg cggcggagct catgtacggt ttgatacatg
     1741 gatatgacgc aaagagctgc ctgcccggca tccgccttga cagtatcaag atgaaaatga
     1801 acagccatga gcctttgtgg acggatatcg tagtcacatt ttccaatctg cacggcaaat
     1861 tgaaggcaga accgagtgcc atgggttttg aactggtttc cggaggcaca aagctgggag
     1921 acttcattta caatgtctcc ttgattgaag aaaccgctat tgtgcgcgtt aatatgtcta
     1981 ttgattttat actgcagaac gatgtacata tcgcctatgg atacggattg aatccaaggt
     2041 gtaacattac ggatgaggaa ggatattcga ttcctgcctt tggtcctgtt aaattgagta
     2101 atgatgaata ggctcgggtc agtttgacct gagcttcatt tttgaatggg aggttgtgca
     2161 tagtgaccac aagagagagg atgtatcgca tacttaatca caaggaagct gacagggtgc
     2221 ctattgtgga tcaaccttgg gctggaacga taagaaggtg gataagggaa ggaatgcctg
     2281 ataatgtgga ttacagggat tacttcaata tagacaaagt tgaaagaatt ggagttgaca
     2341 tttctcccag ataccctgtc aagctagttg aagagaccga cagatatgtt atctacacaa
     2401 ctggctgggg agtaaccctg aagcagttca aggaagagga ttcaaccccc gactttattg
     2461 atttcaaggt aaactcgtcc gctgcgtggc aggatgccaa aaagagaatg ctggacataa
     2521 atgaggggaa ggtagatttt gcatatcttg aagccaatta cccaagatgg gtagaaaacg
     2581 gaagttttat tcaagcggat ttctggtttg gctttgatgt agctcattca tggatgtcag
     2641 gtactgaaac catcctcgtt gcaatgcttg aggaacctga atggg
//
LOCUS       DAONVY010000327         2673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_25974_length_2673_cov_3.281895, whole genome shotgun sequence.
ACCESSION   DAONVY010000327 DAONVY010000000
VERSION     DAONVY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25974_length_2673_cov_3.281895"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="PLI11_08420"
     CDS             complement(<1..1750)
                     /locus_tag="PLI11_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ52927.1"
                     /translation="MKRMIENFKVRKQERIAFKKELKTLKSDTLKAYKDFQKLFEDKN
                     RYVSLNEMLDWQDRHEELHDRVSIILQSKDFKYILRVFRDDLNMIIRMFKLIEKYMKR
                     HNKPVVDKMIGSVDQSIFNINGVILNKEQKTAVVVDEKRLRLIGAGTSGLTHTCVGKV
                     KYLVKGKNVPEDNILFLTNSGKNADKVNRMLEKEGISPIAKTYIDYSAQVLADHGKEV
                     PDVVDVDYEEFAESTIKKLLEEEEYKSRFVDYYLNFRQAGSVVFDFDDYEDYLQFLEE
                     YPPIALDGTRMKNYEHLAVADFLYRNNIRYRYMQDYCVRELDYDGTSYKPVFYLPDFD
                     IYIEVFEINAAGEARFDTGTSLVSETPTEIYKKRMQRIREIHEKNHTVMVDVFTYELM
                     ESTLVDHLQTKLMKHLAVFAPMSANALLKIILERNPFLIREISVVLGELLQLARDNSE
                     TWQEIIFLNRESMKTKTRWYKRNESVFSLLMPVYETYLNYIGDNTDAFGIVQMASETI
                     RLQRRSLRYTHIIVDDFHNTNRHMLNLLRNVWHIVMDKASIIVAGNDWQTKSGICAYD
                     FSYFTEFSRFFGHSESI"
     gene            1990..2616
                     /gene="trmB"
                     /locus_tag="PLI11_08425"
     CDS             1990..2616
                     /gene="trmB"
                     /locus_tag="PLI11_08425"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPZ52928.1"
                     /translation="MRMRTKKNLIPRLERVSHLICSKPEERKGKWASGRPLHLEIGCG
                     KGDFISELAERNPDIQYVALEVVKEAAVMALEKVDEKKLANVIFILEDAKNISEYFDK
                     GEVDRIYLNFSDPWPKKKKSKRRLTHPDFLKVYKEILNEDGEIHQKTDNLDFFEFSLE
                     SYKEMDYTLYETTYDLHSTDFRGNIMTEYEKRFVAEGKPICRVVAKLK"
BASE COUNT          832 a          564 c          459 g          818 t
ORIGIN      
        1 taatggactc ggagtgtcca aagaatctcg aaaactccgt gaaatatgaa aagtcgtaag
       61 cacaaatacc actcttggtc tgccagtcat tacccgcaac aattatactt gccttatcca
      121 ttacaatgtg ccatacattc ctgagcaagt tcagcatgtg tctgttcgtg ttgtgaaaat
      181 cgtcaacaat aatatgtgta taacgaagag accttctctg cagcctgatg gtctcggatg
      241 ccatctgtac aataccaaat gcatccgtat tgtcaccgat atagtttaaa tatgtttcgt
      301 atactggcat aagcaatgaa aacacacttt catttctctt gtaccatctg gtctttgttt
      361 tcatgctttc tctgttcaag aaaatgattt cttgccatgt ttcagagttg tctctggcca
      421 gctgcaacag ctcgccaagc actactgaaa tttcacggat taaaaacgga tttctctcaa
      481 gaatgatttt taaaagagca ttcgcactca tgggagcaaa cacagccaaa tgcttcatga
      541 gctttgtctg caagtggtca accagagtgg attccataag ctcgtaagta aacacatcca
      601 ccatcacagt gtggttcttt tcatgaattt ctcttatacg ctgcatcctc tttttatata
      661 tctctgtagg tgtctctgaa accagggacg tacccgtgtc aaatctggcc tcacctgcgg
      721 catttatttc aaacacctca atatatatat caaagtctgg cagatagaaa actggcttgt
      781 atgaagttcc atcataatcc agttctctaa cgcaatagtc ttgcatgtac ctgtacctta
      841 tattgtttct gtacaggaag tcagctacag caagatgctc gtaattcttc attctggttc
      901 cgtccagtgc aataggagga tattcctcca gaaactgcaa ataatcctca taatcatcaa
      961 aatcaaaaac aacactacct gcctgcctga agtttaggta atagtctaca aacctggact
     1021 tgtactcctc ttcctccaac aactttttaa ttgtgctttc tgcaaattct tcatagtcca
     1081 catcaacaac atcaggcacc tcttttccat ggtcggcaag cacctgagca ctgtagtcga
     1141 tatatgtttt ggcaatgggc gaaattccct ctttttcaag catccgattt actttatcag
     1201 cattctttcc gctattggtc aaaaacaata tattatcctc aggcacattt ttacccttta
     1261 ccagatactt cactttacca acacaggtat gtgtaagtcc cgatgtacct gcaccaatta
     1321 gtctgagcct tttttcatcc actacaacag ctgttttttg ttccttgtta agaatcacac
     1381 catttatatt aaatatgctt tgatctacag aaccaatcat cttgtcaaca acaggcttgt
     1441 tatgtctttt catatacttt tcaatcaatt tgaacattct gattatcatg ttcagatcat
     1501 ctctgaacac cctgagaatg tatttgaaat ccttggactg cagaattatg gacactctgt
     1561 catgaagctc ctcatgcctg tcctgccaat cgagcatttc attcaaggag acatatctgt
     1621 tcttgtcttc aaagagtttc tggaaatcct tataagcttt caatgtatcg cttttcagtg
     1681 ttttgagttc ctttttgaaa gctattcttt cttgcttcct taccttaaaa ttctcaatca
     1741 ttcttttcat aacatccaac tccaaacaag tattctccat tcagcccatc tgactttatc
     1801 ttaccatatt atcagtaaag tttctataaa taaatggatg tgtatagtat ctggaatgcg
     1861 attggtatat aaatatatgc aagtatggca attatgtgct gcaaacaata ctactatgaa
     1921 ccttgacaac aaattttaga cggatataat ctcacatgag caataattaa taacggagaa
     1981 gatttgcaaa tgaggatgag aacgaagaaa aacctaatac caagacttga aagagtatcc
     2041 cacttaatat gtagcaaacc agaagaaagg aaaggaaaat gggccagtgg cagaccactg
     2101 cacttggaaa tcgggtgtgg caaaggcgat ttcatatcgg agcttgccga aagaaaccct
     2161 gacattcagt atgttgcact ggaggttgta aaagaagccg ctgtcatggc acttgagaaa
     2221 gttgatgaaa agaaacttgc caatgtcatc ttcatactgg aagatgccaa gaatatatcc
     2281 gaatattttg acaaaggcga ggtagacaga atctatctca atttcagcga cccatggcca
     2341 aagaagaaaa aatcaaaaag aagactcacc catcctgatt ttcttaaagt gtacaaggaa
     2401 atactgaatg aagatggtga gattcatcaa aaaaccgaca atttagactt ttttgagttt
     2461 tcactggaat catacaagga aatggactat accctgtatg aaacaacata tgatttgcat
     2521 tccactgatt ttcgtggcaa tattatgaca gaatacgaaa agaggtttgt ggcagagggt
     2581 aaacccatct gcagagttgt tgcaaaactg aaataagtat aggctttgta aactaaacat
     2641 gaaaaaatag cacaatgttt tatagaattg ctt
//
LOCUS       DAONVY010000328         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26047_length_2667_cov_3.022205, whole genome shotgun sequence.
ACCESSION   DAONVY010000328 DAONVY010000000
VERSION     DAONVY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26047_length_2667_cov_3.022205"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLI11_08430"
     CDS             <1..771
                     /locus_tag="PLI11_08430"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HPZ52929.1"
                     /translation="TGEVFHLWVIEGDKNILNVLPFDKLDLNVILTNDLTPYRDRKVR
                     ILNGAHTMTVLAAYLYGKDTVKECVDDELVNLYMRKGIFEEIIPTLDLPKEDLIDFAN
                     SVLERFANPFIQHYLLSISLNSVSKFRTRVLPSILEYAKRKGRIPQVMSFSFAALMAF
                     YRGERLKNDCLVGYRKGIEYEIRDDVNNLVMFKELWDGFDGTRKSVERIVKTVFSKKA
                     LWDMDLNEVEGLADLVAGFLFDIVTSGIENTMKGLVYG"
     gene            764..2245
                     /locus_tag="PLI11_08435"
     CDS             764..2245
                     /locus_tag="PLI11_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HPZ52930.1"
                     /translation="MVNRNTALIIHEKDNVLCALNDIKKGEAVLDFRALEDIPKGHKI
                     ALLDIEKGEEVVKYGFPIGRALKPIKRGSFVHTHNLTTNLEGVLDYTYNPVTVDEDVL
                     EYATFMGYEREDGSVGVRNEIWIINTVGCVNKTAELLARQANERFKGKVDGVFSFCHP
                     YGCSQLGQDALVTQKILAGLVHHPNAAGVLVLGLGCENNNIDVFKKVLGNTNDKRVKF
                     LETQKVEDEIEEGLRLINVLVEYAQTFERTECPASNLILGLKCGGSDAFSGITANPLV
                     GKISDRLVAMGGTSVLTEVPEMFGAENILMDRCINKDVFDRLVAVVNGYKEYFLKHGQ
                     VVYENPSPGNKEGGITTLEEKSLGCVQKGGTSAVVDVLKYGDRVEKNGLNILEGPGND
                     MVAQTVLAASGAQIILFTTGRGTPLGAPVPTIKISTNSELYTRKRKWIDFNAGKLLES
                     ESMEDMCKELFVYILDVASGNVYTCNEKYGYREIAILKDGVTL"
     gene            2262..>2667
                     /locus_tag="PLI11_08440"
     CDS             2262..>2667
                     /locus_tag="PLI11_08440"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPZ52931.1"
                     /translation="MSKFIHDDFLLSNDSAKILYHTYAKDMPIIDYHCHISPKDIAEN
                     RKYKNITELWLGGDHYKWRAMRSNGVDEKYITGDALDEEKFSKWADTVPYTLGNPLYH
                     WTHLELERYFGISKLLGPDTREEIWMQCNEMLK"
BASE COUNT          849 a          398 c          680 g          740 t
ORIGIN      
        1 acaggggagg tattccattt gtgggtgata gagggcgaca aaaatatttt gaatgtcttg
       61 ccatttgaca aactggattt gaatgtaata ttgacaaatg acttaactcc gtacagggac
      121 cgaaaggtta gaatattaaa tggtgcacat accatgacag tgctagcagc ttacctttat
      181 ggaaaagaca ctgtaaagga gtgtgttgat gatgagcttg taaatttata catgagaaag
      241 ggtatatttg aagaaataat tccaacgctt gatcttccca aggaagattt gatagatttt
      301 gcaaacagtg ttctagaaag atttgcaaat ccgtttattc agcactattt actcagtatc
      361 tcgttaaatt ctgtttccaa gttcagaaca agagtattac cttcaatact tgagtatgca
      421 aaaaggaaag gaagaattcc ccaagttatg tctttttcct ttgcagcctt gatggctttt
      481 tacagaggtg aaaggttgaa aaatgattgt cttgttggct acagaaaagg tattgagtac
      541 gagataaggg atgatgtgaa taatcttgtt atgttcaagg agctttggga cgggtttgac
      601 ggaactagaa aatcggtgga gagaatagtc aaaactgtct tttcgaagaa agcgttgtgg
      661 gatatggatt tgaacgaggt tgaaggtttg gcagaccttg tcgcaggctt tttgttcgat
      721 attgtaacaa gtggaataga aaacacaatg aaaggattgg tttatggtta atcggaatac
      781 ggccttgata atacatgaaa aggataatgt attgtgtgct ttgaacgaca ttaaaaaagg
      841 agaagctgtt ttggattttc gtgctcttga agatatccca aaagggcata aaatagccct
      901 tttggatatt gaaaaaggag aagaggttgt aaaatacggt tttcctatag gaagggcgtt
      961 aaaacctata aagagaggtt cttttgtaca cacgcacaat ctaacgacaa atcttgaagg
     1021 agtgctggat tatacataca accctgtaac agttgatgaa gatgttttgg agtatgcgac
     1081 attcatggga tatgaaaggg aagatggcag cgtaggtgta aggaatgaaa tctggataat
     1141 caatactgtg gggtgcgtaa acaaaactgc ggaactgctt gcaaggcagg caaatgaaag
     1201 gtttaaaggc aaagtggatg gtgtgttttc tttctgtcat ccttatggat gctcgcagct
     1261 gggacaggat gctcttgtaa cccagaagat acttgcaggt cttgtccatc atcccaatgc
     1321 agccggtgtt cttgtgctgg gtcttggatg cgagaataat aatattgatg tgttcaagaa
     1381 ggtgcttggc aatactaatg acaaacgagt gaagtttttg gaaacccaaa aagtagagga
     1441 tgagatagag gaaggattga gattaataaa tgtgcttgtt gagtatgcac aaacctttga
     1501 gcgtacagaa tgccctgctt caaatcttat tcttggtcta aaatgcgggg gcagtgatgc
     1561 attttcaggc attacagcaa atcctttggt ggggaaaatc tctgacaggc tcgttgcaat
     1621 gggtgggact tctgttttga ctgaagtgcc tgagatgttt ggtgccgaaa atattctaat
     1681 ggacaggtgt atcaacaagg atgtgtttga caggcttgtc gctgttgtaa acggatacaa
     1741 ggaatatttt ctaaagcatg gacaggtagt atatgagaat ccttcgcctg gaaacaagga
     1801 aggaggcata acaactcttg aggaaaaatc tttaggctgt gtacaaaagg gtgggacaag
     1861 tgctgttgtg gatgtattaa aatatggtga cagggtcgaa aagaatggtc taaacatatt
     1921 ggaaggtcca ggcaatgata tggttgcaca gacagttctg gcagccagcg gtgcccagat
     1981 aatactgttt accacgggca gaggcacgcc tcttggagct ccggtgccta caatcaagat
     2041 atctacgaat agcgaattat atacaagaaa gaggaaatgg atcgatttca atgcaggaaa
     2101 acttcttgaa tccgagagta tggaagacat gtgcaaggag ctatttgtat atatacttga
     2161 tgtggcttct ggtaatgtgt atacatgcaa tgaaaaatat ggctacaggg agattgccat
     2221 actaaaagat ggagtgactt tgtaaggaag gagaagatgt tatgagtaag tttattcatg
     2281 atgatttttt gctgagtaat gactcggcaa agattctgta tcacacgtat gcaaaggata
     2341 tgccgataat agattaccac tgccatatta gccccaaaga tattgcagag aacagaaagt
     2401 acaagaacat aaccgaactt tggcttggcg gcgaccacta caagtggagg gcaatgaggt
     2461 caaacggtgt tgatgaaaag tacataacag gagatgcact tgacgaggaa aagttttcta
     2521 aatgggcgga cacagttcca tatactcttg gaaatcctct ttaccactgg actcatcttg
     2581 aattggagag gtattttggc ataagcaaac tgcttggacc ggatacgagg gaggaaatct
     2641 ggatgcaatg caatgaaatg ctaaagt
//
LOCUS       DAONVY010000329         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26054_length_2667_cov_1.922282, whole genome shotgun sequence.
ACCESSION   DAONVY010000329 DAONVY010000000
VERSION     DAONVY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26054_length_2667_cov_1.922282"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLI11_08445"
     CDS             <1..306
                     /locus_tag="PLI11_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HPZ52932.1"
                     /translation="IGEGLGQNWWCVLFPPLCFVNANVNAAINEPDADNNTEEEVDEN
                     EINEQGKEALKDNLSKTSYEVIEKPNNGIKFKFKIIEVIENAKMRIQATFKRALKFR"
     gene            422..1192
                     /locus_tag="PLI11_08450"
     CDS             422..1192
                     /locus_tag="PLI11_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HPZ52933.1"
                     /translation="MAVAHKGAISFGLVHIPVNLYVAAERVGISFNQLHKECMGRIRY
                     KKSCPACKLDEVSKDDIVKGYEYEKNKYIVIEEEELEKIKTEKDKTIYILNFVSLSEI
                     DPIYYEKTYYVAPDGSDKAYMLLAEAMKSEGKVAIAKTVMGTKEKLLTIRSTGAGLLI
                     ETMFFEEEIKTMPKPIGDVNLNEQELQMAKILINNMTTEFKPERYKDEYTMRLKEAII
                     RKVNGAEIIQPQQEGAGAIVNLMEALQQSIKATEQWVQ"
     gene            1194..2186
                     /locus_tag="PLI11_08455"
     CDS             1194..2186
                     /locus_tag="PLI11_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HPZ52934.1"
                     /translation="MKDIFDKKDIKPMLLTERNESFNSPDYLYELKLDGIRTIIYVDK
                     GNVEIRNKRNMRLNQTYPELADICGQIKQKCILDGEIIAVKDGKPDFYAIQKRSMMTN
                     KTKIEFASKMYPVSFSAFDILYIGSEQITGKPLVERKQILSENIVENEKIIVSRYIFE
                     KGIELYNAAASQDLEGIVAKKKDSKYYFGKRSKDWIKIKNLKDDDYVICGYVVKPGNK
                     VSLILGAYNEGTLVRQGHVTMGVTREDFNKVLSVNRLNKPLFDDPSGNEATWIEPVLV
                     CTVQYMMKSEHGQMRQSVFKGIRNDKDPEQCVVKETEYIGLLLQILKLNQNPGG"
     gene            2190..>2667
                     /locus_tag="PLI11_08460"
     CDS             2190..>2667
                     /locus_tag="PLI11_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52935.1"
                     /translation="MGILSKINNVLKYKEPKQYYKFRFLEDEEKDRGEIDHVAVGAEN
                     KQVYVPQEKDNISSEPEQKGQSDQNSKQDKKSDKQQDQNKNQNNGQDENKSTDKTANN
                     STEQNHAKSNEQAKNENKQQSSGKSRKKEMKKPIEVKTIGENKNPCDMKEVSRNLKE"
BASE COUNT         1032 a          429 c          608 g          598 t
ORIGIN      
        1 ataggagaag gccttggcca gaactggtgg tgcgtcttgt tcccgcctct ttgttttgtg
       61 aatgcaaatg tcaatgcagc tataaatgaa ccggatgcgg acaataacac cgaagaagaa
      121 gtggacgaaa atgagataaa tgaacaaggg aaagaagcgc tgaaagataa tttgtccaaa
      181 acatcttatg aagttattga aaagcccaat aatggcataa agtttaagtt caagataatc
      241 gaagtaattg agaatgcgaa aatgagaata caggcaacat tcaaaagagc tctcaaattc
      301 agatagcaaa gcaaaattaa gtctgcattt ctgaaagggc cggcataaaa accgggttct
      361 ttcatttcag cgtatttatt atcctctttt gcaaataata aagcgaaagg gggtatttta
      421 tatggctgtt gctcataaag gagcaatttc gtttggcttg gtgcacatac ctgtaaatct
      481 ttatgttgca gcagaaaggg ttggcatttc atttaaccag ctgcacaagg aatgcatggg
      541 taggataaga tataaaaaga gttgtcctgc gtgcaagttg gatgaggtct ccaaagacga
      601 tattgtaaaa gggtacgagt atgagaaaaa caagtatatt gtaatagaag aggaagagtt
      661 ggaaaagata aaaacggaaa aagacaagac catatatatc ttaaactttg tatctttgtc
      721 cgagatagat ccaatatact acgaaaaaac gtattacgtt gccccggatg gcagtgacaa
      781 ggcatacatg ctcctggccg aagcaatgaa aagtgagggg aaagtcgcca tagcaaaaac
      841 tgtaatgggg acaaaagaga agcttctgac aataaggtcg acgggagcag gtttgctcat
      901 tgagaccatg tttttcgaag aggaaataaa gaccatgcca aaaccaatag gtgatgtcaa
      961 cctaaatgag caggagctgc aaatggctaa aatattgata aacaatatga caaccgaatt
     1021 caagcctgaa agatacaagg atgagtacac aatgaggtta aaagaagcaa tcataagaaa
     1081 ggtaaatggt gctgaaatta tacagccaca acaggaaggt gctggagcaa ttgtcaatct
     1141 catggaagcg ctgcagcaaa gcataaaggc tacggagcag tgggtgcagt aatttgaaag
     1201 acatatttga taagaaagat ataaaaccca tgctgttaac agaaagaaat gaatctttca
     1261 actctccaga ctatctctac gaactaaaac tcgatggtat acgcacaatc atatatgtag
     1321 acaaggggaa cgttgaaatt cgtaataaaa gaaacatgcg gctcaatcag acatacccgg
     1381 aactggcgga tatatgtggg caaataaagc aaaaatgcat attggatgga gaaataattg
     1441 cggtcaagga tggtaagccg gatttctacg caatccaaaa aagatccatg atgacaaaca
     1501 aaaccaaaat agagtttgcc tcgaaaatgt acccagtatc cttttctgct ttcgatattt
     1561 tatacattgg ctctgagcaa ataacgggca aacctttggt ggagagaaaa caaatcttga
     1621 gtgaaaacat tgttgagaat gaaaaaatca ttgtttcgag atatatattt gaaaaaggaa
     1681 ttgaactata taatgcagct gcaagccagg atcttgaagg aattgttgca aagaaaaagg
     1741 acagtaagta ttatttcggc aaaagatcaa aagactggat aaaaattaaa aacttaaagg
     1801 acgacgacta tgttatctgc gggtatgtag taaaaccggg aaacaaggta agcttgatac
     1861 tcggagcgta caatgaaggg acccttgtaa gacaaggtca tgtcaccatg ggtgttacaa
     1921 gagaggattt taacaaggtc ctgtcagtca atagacttaa caaaccgctt tttgacgacc
     1981 cttcaggtaa cgaggctacg tggatagaac ctgtgctggt ttgcactgta cagtatatga
     2041 tgaagtcaga acatgggcag atgaggcagt cggtgttcaa aggcataagg aatgacaagg
     2101 accctgaaca gtgtgttgta aaagaaactg aatacattgg cttattgcta caaatactca
     2161 aattaaacca aaatccggga ggatagctta tgggcatatt atccaagata aacaatgtat
     2221 tgaaatacaa ggaaccaaaa caatattata aatttagatt tcttgaagac gaagaaaagg
     2281 atcgtggaga aatagaccat gttgcggtcg gtgcagaaaa caagcaagta tatgtccctc
     2341 aggagaagga taatatcagc tcagagccag agcaaaaggg gcagagtgac caaaactcca
     2401 agcaggataa aaagtcagac aagcagcagg accaaaacaa gaatcaaaac aatggtcaag
     2461 atgaaaacaa gagcaccgat aagacagcaa ataatagtac cgagcaaaac cacgcaaaat
     2521 ccaacgagca agcaaagaat gaaaacaagc agcagtcttc gggtaaatct agaaagaagg
     2581 aaatgaaaaa acctatagaa gtaaaaacca taggcgagaa taaaaatcca tgtgatatga
     2641 aagaggtttc aaggaatctt aaggaaa
//
LOCUS       DAONVY010000330         2645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26337_length_2645_cov_2.716216, whole genome shotgun sequence.
ACCESSION   DAONVY010000330 DAONVY010000000
VERSION     DAONVY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26337_length_2645_cov_2.716216"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="PLI11_08465"
     CDS             complement(<1..1714)
                     /locus_tag="PLI11_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52936.1"
                     /translation="MKMYEYQSFQESDKKVLMFYHLWHGCFTESAVNIDIHAPRNITR
                     TIKENRDALNNEDILGPYISYHYWGEPLFGYYDLAKDEYVIRKHAQMLTDGGVDGIVI
                     DYSNYMGGRGKAYTKNELTNLLETYLKMREEGNRTPDVAIMCTWSAEEGARAANDFYE
                     DFYSKDKYYDIWFKWEGKPLILVHPQKISYELKDFFTTRRPYPFLTPVDEENAWPWLS
                     LYPQKCGYTKDNPKEVVSVGIAQNWSDSLDFFSARDEKGHFITRGRSWSSKGHKLLKD
                     PTSSLYESHKGANFNECFERAIELDPNVIFVTGFNEWIAARFKNVPSWARMSKDTTPP
                     FGAFCDAFTTEFSRDIEPTREEELKDSFYLMLVENVRRYKRVESKTDIIVPIRIEIDG
                     NFEDWDGVENEYHDDVNDIANRHAQGIGRHVYVNTTGRNDFKLMKVAYDNDYIFFFCE
                     TVSDITPYTDENWMNLLLHVSFDRPHWEGYNFIVNRTGVRNNHTTLEKSLGGFNWEVV
                     DDKIPYRVKGNKLELAIKRNVLGVCDKDIDFMFKWCDNIKYEDILNFYTDGDTAPNGR
                     FNYRF"
     gene            complement(1729..2457)
                     /locus_tag="PLI11_08470"
     CDS             complement(1729..2457)
                     /locus_tag="PLI11_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HPZ52937.1"
                     /translation="MRKTWFLYFLVLILILSGCNSESGDKDGAKSTPTPAIRTEAPGN
                     TEEPDEDPTAEPTEEPTPEPVPTVEPGKETYAEGIKDPDKGIRYTFRPSKGRADHIMI
                     NESLATQFFATTTFNKIELECPSYHDNIGTIVMELYRWMGSYDLTLDKESNPPLAVKE
                     FVDYADNALLAMEFDEPLVDGEYLLYLTTPDPTEQVGVWATYEAENLHTRTYKDNEEF
                     EGVGFGPIIYYTKTPNKLYGPISG"
BASE COUNT          681 a          565 c          532 g          867 t
ORIGIN      
        1 aaaacctgta attgaacctt ccattgggag ctgtgtcgcc atcggtgtaa aagttgagaa
       61 tatcttcgta cttaatgttg tcgcaccatt taaacataaa gtcaatatcc ttgtcacaaa
      121 cgccaagaac atttctcttt atagccagct ccagcttgtt gccttttact cggtacggta
      181 ttttatcgtc tacaacttcc cagttgaaac ctcccaagga cttttcaaga gttgtgtggt
      241 tattcctgac tcctgttctg tttacgataa aattgtaacc ttcccagtgg ggtctgtcaa
      301 aagatacatg caaaaggaga ttcatccagt tctcgtcagt atatggtgta atgtcagaca
      361 ctgtttcaca gaagaagaat atgtaatcat tgtcataggc aactttcatt agtttaaaat
      421 cgtttcttcc tgttgtgttt acatagacat gtcttcctat accttgagca tgtctgtttg
      481 caatatcatt aacatcgtcg tgatattcat tttctacacc atcccaatct tcaaaattac
      541 cgtcgatttc aattctgatt ggaacaatta tatcagtttt actctccacc ctcttatacc
      601 tgcgaacatt ctccaccagc atcaaataga aactgtcttt gagttcctct tctctggtgg
      661 gttcaatgtc acggctgaac tccgtggtaa aggcatcgca aaaggcccca aaaggtggag
      721 ttgtgtcctt tgacattcgt gcccaactgg gtacattttt aaatcttgca gcaatccatt
      781 cattaaagcc agttacaaaa atgacgtttg gatcaagctc tatcgctctt tcaaagcatt
      841 cgttgaaatt tgcaccttta tggctttcgt aaagactgct ggtaggatcc tttagaagct
      901 tgtgcccttt tgaagaccag cttcttcctc tggttataaa gtggcctttt tcatctcttg
      961 cactgaaaaa gtcaagacta tcagaccagt tttgggcaat tcctacagaa acaacttctt
     1021 tcgggttgtc cttcgtatac ccgcattttt gtgggtatag tgaaagccag ggccaggcat
     1081 tttcctcgtc aacgggtgtc aagaaaggat agggccttct tgtggtaaag aagtctttga
     1141 gctcatatga aatcttttga ggatgtacaa gtataagggg cttgccttcc cacttgaacc
     1201 aaatatcgta atatttgtcc ttagagtaaa agtcttcata aaagtcattg gcagccctgg
     1261 caccttcttc tgcgctccag gtgcacatta tggctacatc gggtgttctg tttccttctt
     1321 ccctcatttt taaatatgtc tctagcagat ttgtgagctc attttttgta taggcttttc
     1381 cgcgacctcc catgtaattg gagtagtcaa ttacaatgcc gtccacgccg ccatctgtta
     1441 gcatttgtgc atgctttcta ataacatact catccttggc aagatcatag taaccaaaca
     1501 agggctctcc ccaatagtga taggagatat aggggcccaa aatatcttcg ttattaaggg
     1561 catctctgtt ctcctttatt gttctggtta tgttccttgg ggcatgaatg tcgatattaa
     1621 cagcactttc tgtaaaacat ccatgccaca agtgataaaa catgagtact tttttgtctg
     1681 attcttgaaa ggattggtat tcatacattt tcattatatt gctttccttt agcctgaaat
     1741 tggtccgtaa agtttgttgg gcgtctttgt gtagtagatt attggaccaa aacctactcc
     1801 ttcaaattcc tcgttgtcct tgtatgttct tgtatgcaaa ttttccgctt cataggttgc
     1861 ccaaacccct acctgttctg tcggatcagg agtggtgaga tagagaagat actcgccatc
     1921 aaccaagggt tcgtcaaact ccattgcaag aagagcattg tcggcataat caacaaattc
     1981 ttttacggca agcggaggat tgctctcctt gtcaagggta aggtcatatg aacccatcca
     2041 tctgtaaagc tccattacaa tagtaccaat attgtcatga tagcttggac actccaactc
     2101 aatcttgttg aaagtggttg tggcaaagaa ctgtgtagcc agggattcgt tgatcatgat
     2161 gtggtcggct cgtcccttcg aaggcctgaa ggtgtatctt atacccttgt caggatcctt
     2221 tattccttct gcataggttt ccttgcctgg ctcaactgtt ggaaccggct cgggtgttgg
     2281 ttcttcagta ggttctgctg ttggatcctc gtcaggctct tctgtattac ccggagcctc
     2341 ggttcttatg gcgggagtag gtgttgattt ggcaccatcc ttgtcgccgc tttcagaatt
     2401 gcagcctgaa aggataagga ttagaaccag aaagtataaa aaccaggttt ttctcatttg
     2461 tttaaccccc ttttgtaata ctacgatatg tgtaaataaa tcatatcaca cggaaaggtt
     2521 taataaaggg gagaaattca accagatttt ttaggctatt tcaacacatt gtgtgtggta
     2581 tataataaat tggtccgagt aaaagatgag aggtgcaagt catgaaggtt ttcaagttct
     2641 gcgag
//
LOCUS       DAONVY010000331         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26442_length_2636_cov_3.394808, whole genome shotgun sequence.
ACCESSION   DAONVY010000331 DAONVY010000000
VERSION     DAONVY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26442_length_2636_cov_3.394808"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2494
                     /locus_tag="PLI11_08475"
     CDS             <1..2494
                     /locus_tag="PLI11_08475"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPZ52938.1"
                     /translation="DMRKWVGWCKDRGFGGFALIFSNPVDSPVLPDYWYDKLLASARV
                     LVEEAKKLDLEVWIFDEWGYPSCVAAGLVCENKELRAKKLHVSYDRFLEPGETISIEI
                     PDRTVSLKSIPVNRYGFYAPAGRCNDIAEENSTGDIVKYTSKGYERVILVTWEYVSFI
                     SHVPITNDPLNFSLGTPDLMNKEAASRFIKVVHEKFYDELKDYFGTVIKGFFYDEPEV
                     CFDFPWTQGFENEFIKRKGYDLRDNLPILMAYAGNFYHPGYHEADLIIKNLVFDYFDV
                     WTDLVAENFYGEIQKYCHKRGVLSVGHQDMDNHTKTLASVSGHFFKNSYYNDSPGIDV
                     IGSNIDIDTFYDFPRFAGSCKRLYGKNSAMSETFAIMGIAHSPDRMRYLMEHQVIRGI
                     DTFFNMISSIDYEEDTYDLFKPGNYINDLHGRNVNEHVKRAIGLANAGTPASDLAVYI
                     PMRDIYAHLIRDNDAHNMNSILPYDEVDRIARILAYMPCDFDYIWDEAICELKIDEGF
                     VTAKGQVIRTIIIPPGATIDDKVMVRLKRFVKSGGNVISILQRYANEFILCSDYFLLD
                     EYVRPTVSLKSDNKQVSMCVRRDKDRLVYLFLNESAKKSDCEISFMVMGDEVQVYSMD
                     MNTGKMYVEDSIFSESKRVVRSVFEPGELKCYIVSNAKLDAEKKLKLKVVKDSIKCVT
                     INLPNGTRYITDAKKLPSWADLGYPGHSGFIEYEIEFEWKGKRAFVDFGKVRHCLVFS
                     LDKRVHPFEEGEKISFAPFKADLGKIEEGRHILYVYVQNTVVNRYIGTKDMEKMFDIP
                     QRIDSDRKMLESGLFGEIQLFEEEDLVCQGQR"
BASE COUNT          815 a          395 c          633 g          793 t
ORIGIN      
        1 cgacatgaga aaatgggttg gctggtgtaa ggatagaggt tttggaggat ttgctttaat
       61 cttttcaaat cctgttgact cccccgtttt gcctgattac tggtacgata aactgttggc
      121 ctctgccaga gtattggtgg aggaagccaa aaaacttgac ttggaagttt ggatttttga
      181 tgaatgggga tatcctagct gcgtggcggc aggtcttgtc tgtgagaaca aggagttaag
      241 agccaagaaa cttcacgtat cttacgatag atttctggaa cctggcgaga caattagtat
      301 tgagatacct gacaggacgg tttcattgaa gagtattcca gtgaaccgtt atggttttta
      361 tgcgcctgca ggccgttgta acgatattgc agaagaaaat tcaacaggtg atattgttaa
      421 gtacacaagc aaggggtatg aaagagtcat attggtaacc tgggagtacg tatcatttat
      481 ttcccatgtt cctataacga atgatccttt aaatttcagt ttgggtactc ctgatttaat
      541 gaacaaggaa gcggcaagta gatttattaa ggtcgtacat gaaaaatttt atgatgaatt
      601 gaaggattat ttcggtacgg taataaaagg gtttttctat gatgagcctg aggtttgctt
      661 tgattttcca tggacgcaag gttttgagaa cgagtttatt aaaagaaaag gatatgactt
      721 aagggacaat cttcctattc ttatggcata tgctggaaat ttctatcatc ccggttacca
      781 tgaggcagat ttgattatta aaaacctggt atttgattac tttgacgtat ggaccgatct
      841 ggtagcagaa aacttctatg gtgagataca gaagtactgc cataaaagag gagtcttgtc
      901 ggtaggacat caggacatgg ataatcatac aaaaactctt gcgtcggtaa gtggtcattt
      961 ctttaaaaac agttattaca atgattctcc tggtattgat gtgataggca gcaatattga
     1021 tattgacaca ttttatgatt ttccaaggtt tgctgggtcc tgcaaacgcc tttatggcaa
     1081 aaactctgcc atgagtgaaa cttttgcgat aatgggaata gctcattccc cagacaggat
     1141 gagatacctt atggagcatc aggtgattcg aggaattgat acattcttta atatgatatc
     1201 aagtattgat tatgaagaag atacgtatga cttgttcaag cctggcaatt acattaatga
     1261 tttgcatgga agaaatgtaa atgagcatgt aaaaagggca atcgggctgg caaatgcggg
     1321 tacgcctgca tccgaccttg cagtatacat tccgatgagg gacatatatg cacatttgat
     1381 aagggacaat gatgcacaca atatgaattc aattcttcca tatgatgagg ttgacaggat
     1441 agcaaggata ctagcataca tgccctgcga ctttgattat atatgggatg aagctatatg
     1501 tgagctgaaa atagatgagg gatttgtcac agctaaaggt caggttattc gtacaataat
     1561 aattcctcct ggtgccacaa ttgatgataa agtcatggta agacttaaaa ggtttgtgaa
     1621 aagtggtggg aatgtaatat caattttgca gaggtatgca aatgaattta ttctctgttc
     1681 ggattatttt cttcttgatg agtatgtacg gcctaccgta tctttaaaga gcgataacaa
     1741 gcaagtgtcc atgtgtgtga gaagagacaa agatagactt gtttatctct ttctaaatga
     1801 atctgcgaaa aagagtgatt gcgagatatc tttcatggta atgggtgatg aagtccaggt
     1861 atattcaatg gatatgaata caggcaagat gtatgtggaa gacagtatct tctcagaatc
     1921 caaacgggtg gtgaggagtg tttttgagcc tggggagctc aagtgctata ttgtttccaa
     1981 tgcaaaactg gatgcagaga aaaaattaaa actgaaagtt gtcaaagatt caataaaatg
     2041 tgttacaata aacttgccta atggcacaag atatattacg gatgctaaaa aactgccctc
     2101 gtgggctgat ttgggttatc ccggacattc aggttttatc gagtatgaga ttgagtttga
     2161 atggaaggga aaaagggctt ttgtggattt tggaaaggta agacactgcc ttgttttctc
     2221 acttgataaa agagtccatc cttttgagga aggtgaaaaa atttcctttg ctcctttcaa
     2281 ggctgatttg ggcaagatag aggaaggcag gcatattcta tacgtttatg tacaaaacac
     2341 tgttgtaaat agatatatcg gaacaaaaga catggaaaaa atgtttgaca ttccgcagcg
     2401 catagattcg gacagaaaaa tgcttgaatc cggattgttt ggcgaaattc aattgtttga
     2461 ggaggaagat ctggtttgtc aaggacaaag atagtactta ttgccgttat cgtattgttg
     2521 attgcactct tggtagcgtg caaggatata aaggagatac caaaagagaa caatggaaca
     2581 ggcaactatc ctgagacttt tgatctgcag cgacctgttt tttcagatgt gggagg
//
LOCUS       DAONVY010000332         2631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26500_length_2631_cov_3.520575, whole genome shotgun sequence.
ACCESSION   DAONVY010000332 DAONVY010000000
VERSION     DAONVY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26500_length_2631_cov_3.520575"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLI11_08480"
     CDS             complement(<1..945)
                     /locus_tag="PLI11_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPZ52939.1"
                     /translation="MKRTANLTEGHIAKTLFKLALPIMGTSFVQMAYSFIDMIWVGKI
                     GSDAVASVGTVGFFTWLASAFVLVPKIGAEVNVAQAVGMKDHERVKEYVRQSLQIGVF
                     LALLYGMVMIVFRNQLIAFFNLGDENVIKDAVDYLVIVSLGFVCFFINPVFTAIFNGH
                     GDSRTPFLINSIGLVINVLLDPLLIFGIGPFPRLEVKGAAIATVIAQVIASSIFLLKA
                     RKLPDVFSDIHFFRRPKAEYVLRISKIGIPSALQSGLFAIIAMIIARIIAGWGTTAIA
                     VQKVGSQIESISWLTAGGFQSAMSAFIGQNLGARKLDRV"
     gene            complement(1116..>2631)
                     /locus_tag="PLI11_08485"
     CDS             complement(1116..>2631)
                     /locus_tag="PLI11_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52940.1"
                     /translation="EPLFGYYDIADEWVVTRHVKMLTDAGVDFLMIDFTNGVWSDYID
                     RLEVLFKVLDKFHKQGFSVPKVVCAAYDDSPRTVDELYSRFYSKNPQYDHLFYRWKDS
                     EKPLMFDNIEGYNKKATGFIDTESKLASYSEGAREFFDFRDLAFPHETKNNNDFGSSF
                     LYLDFRVNPSKVTDGDGFSFCCVSVSEISVTNRASAQWFEDSRDRTRSFDGKENRNWK
                     KGEEDAYLYGYNFKRQFEYAIKEDPDMIFVTGWNEWIAQLQIRSNGEISFVDAADINN
                     SRDIEPMNGGYGDNYYMQLCHYISKFKYGNVKNEPASSISIDVKGDFDQWDNENILTY
                     VDYVDDVFDRNKTSAHNWSLKYTNTTGQNDIKTVKVTSDKNNVYFYVDTVDPIVNFEG
                     ERSMTLFINTGSKTNWYGYDFAVHRTGGSDMIIEKCKGGYEWDEVGKAEYKLSENMLM
                     LSIPRKTLGVSGKEFSISFKWADNFSGDGDIFTFYTDGNAAPYGRLNYLYVGEK"
BASE COUNT          756 a          619 c          461 g          795 t
ORIGIN      
        1 taccctgtcc agttttctgg cgccaagatt ctgcccaata aatgcgctca tggcagattg
       61 aaatccgcct gcggttagcc aggatattga ctcaatctgg gagcccactt tttgaaccgc
      121 tatggcagtt gtcccccagc cagcaattat gcgcgctata atcattgcaa ttattgcaaa
      181 caagccactc tgcaaagcag aaggaatgcc aattttcgat atcctgagta catattcagc
      241 ctttggtctt ctgaagaaat gtatatctga gaagacatcg ggcagttttc tcgcctttaa
      301 gaggaagata ctcgaagcta ttacctgagc tattactgta gctatggcag ctccttttac
      361 ttcaagtctt ggaaaaggac caattccgaa tattagaagt gggtcaagta aaacattaat
      421 aacaagacct attgaattga ttaagaatgg agtcctgctg tctccgtgtc cgttaaatat
      481 tgcggtaaat acagggttaa taaagaaaca gacaaaaccc aaggatacaa taactagata
      541 atccaccgca tccttgataa cattctcgtc ccccaaatta aaaaaagcaa tcagctggtt
      601 tctgaataca atcatgacca ttccatataa caatgcgagg aaaacaccta tctgaaggct
      661 ctgccttaca tactctttta ctctttcatg gtctttcata ccaactgcct gggcaacatt
      721 tacctccgcc cctattttcg gaactaaaac aaatgcgctt gcaagccagg tgaaaaatcc
      781 taccgtacca acagacgcca ccgcatcaga accaatcttg ccaacccaaa tcatatctat
      841 aaagctgtaa gccatttgaa caaagcttgt ccccataatc ggcagtgcca gcttgaaaag
      901 tgtttttgca atatgtcctt ccgtcaagtt tgcagttctt ttcattcgta tcttttcttc
      961 tccttatagt gcatttgtgt gcaaaaaaaa ttagacttat gtaagctgca taagtcgtat
     1021 tatatcattt cttttgtaaa acagggagaa taatgtgtta atttgattca aaaagaaagt
     1081 gcctaaagtt tctaaacctc aggcactttt tataattatt tctcaccaac gtacaggtag
     1141 ttaagtcttc catagggtgc tgcattgcca tctgtataga atgtgaagat atcaccgtct
     1201 ccactgaagt tgtcagccca tttgaagctg attgaaaact ccttgccgga aacacctaaa
     1261 gttttgcgag gaatggaaag catcaacatg ttctctgaca gcttgtattc agcttttccg
     1321 acctcatccc attcgtaacc gcccttgcac ttctcaataa tcatatcgga cccaccggtt
     1381 ctatgaaccg caaagtcgta cccgtaccag ttagtctttg aaccggtgtt gataaacaag
     1441 gtcatgcttc tttctccttc aaagtttaca atcggatcca ccgtatccac atagaaatac
     1501 acattgttct tatccgaagt cacttttacg gtctttatgt cattttgacc tgtggtgttt
     1561 gtatacttca aactccagtt atgagctgat gttttgtttc tatcgaaaac atcatcaacg
     1621 taatcaacgt atgtaaggat gttttcatta tcccactggt caaaatcacc tttgacatca
     1681 atacttatgg atgaagctgg ctcatttttt acatttccat atttaaactt gctgatataa
     1741 tggcaaagct gcatgtagta gttgtcacca tatccaccat tcataggttc tatgtctcta
     1801 ctgttgttta tatctgcagc atccacgaag ctaatctcac cattggagcg aatctgaagt
     1861 tgtgcaatcc attcattcca tccggtaaca aagatcatat ccggatcttc tttaatggca
     1921 tactcaaact gtcttttaaa gttgtagcca taaagatagg catcctcttc gcccttcttc
     1981 cagttcctgt tctccttacc atcaaagctt cgggtcctgt ccctgctatc ctcaaaccac
     2041 tgggcggacg ccctgttggt tacagatatt tcagagacgg aaacacagca gaaggagaat
     2101 ccgtctccat ctgttacctt gctgggattt accctgaaat ccaaatacaa gaagctgctg
     2161 ccaaagtcat tgttgttctt cgtttcatgc gggaatgcaa gatctctgaa atcaaagaac
     2221 tctcttgctc cttcagagta gcttgccagc tttgattcag tatcaataaa tcctgtagcc
     2281 tttttgttgt aaccctcaat attgtcaaac attaggggtt tttcgctatc tttccaacga
     2341 tagaacaaat ggtcatattg tggattcttt gaatagaacc ttgaatacag ttcatcaaca
     2401 gttcgtgggc tatcgtcgta tgcggcgcaa accacctttg gcacactaaa accttgcttg
     2461 tggaacttgt caagcacctt aaacagtact tctagtctgt ctatgtaatc agaccaaaca
     2521 ccgtttgtaa agtcgatcat caaaaagtcc acacctgcat cggtaagcat ctttacatgc
     2581 cttgtaacaa cccactcatc ggcaatatcg tagtagccaa acaaaggctc t
//
LOCUS       DAONVY010000333         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26560_length_2626_cov_3.700506, whole genome shotgun sequence.
ACCESSION   DAONVY010000333 DAONVY010000000
VERSION     DAONVY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26560_length_2626_cov_3.700506"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLI11_08490"
     CDS             complement(<1..430)
                     /locus_tag="PLI11_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HPZ52941.1"
                     /translation="MAINYGFIVPHPPIAIHEVGKGREREIQNTIDAFMSVARMIKEI
                     QPETIIISTPHSTVYSDYIHISPGKSAAGSFSHFGAREVSFKVEYDEELVKAISDFAF
                     EEDIPAGTLGERNRELDHGVMVPLYFINKYYTNYKLVRISI"
     gene            complement(529..1428)
                     /locus_tag="PLI11_08495"
     CDS             complement(529..1428)
                     /locus_tag="PLI11_08495"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HPZ52942.1"
                     /translation="MLSLSEYITISLDTNLFFLRIMKEHAFFLEIAFMQKEDRCIDKA
                     KYFRESYESLLSEAADMAPGRVSRKAVKSEQFVTCHTMDAEEATSCFTCIPFNMSITR
                     KELSLSPNDRRRPLSEQAVTSLNKRAYKLTLEFIEFKEMLLNRVLDCSMFMATYPLLI
                     DHITKEARLFAKRLDTLLRGMHFRDLLGQEIFWNERMEEHMEFVAGLLDPTEEPLICD
                     ARNLAKLFDNLNKKVCKESRPNINSFTKESLDATKKVKQFKNTATNLVLDCKLRSMIM
                     PLLADHILREANHYLFILGAFNS"
     gene            1570..2061
                     /locus_tag="PLI11_08500"
     CDS             1570..2061
                     /locus_tag="PLI11_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52943.1"
                     /translation="MKKAFFFIVLTILTLSMVAVYASNIQITLDKQEYVVGETVTITY
                     SDADGDANAWFAIYPQGEEPYQSYPGTEKVWAYANSNSEQAGSEPVTEGTVTLSTEGL
                     APGEYRCSYFVDIGYNVGATVTLKIVEESTNPETSDAIAYIPFMIGAILSLMVLKRKA
                     VNI"
     gene            complement(2107..>2626)
                     /locus_tag="PLI11_08505"
     CDS             complement(2107..>2626)
                     /locus_tag="PLI11_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPZ52944.1"
                     /translation="GLRDNKIIPTAKHFPGHGDTSVDSHYGLPVVKKSLNDLYGFELV
                     PFMEAIKNNVEMIMTGHIVVKEVDSLPSTLSKKLVTDLLRNEMNYKGVVITDDLGMNA
                     ISDEYSIGQASVMAFNAGCDIILCAQNIEKGKAAYNSLLDAFNRGEVSEERVNESVYR
                     ILKLKERYGIIG"
BASE COUNT          796 a          581 c          458 g          791 t
ORIGIN      
        1 agatggaaat tctgacaagc ttgtaattgg tataatattt gtttatgaaa tacaaaggaa
       61 ccataacacc atggtcaagc tccctgttcc tttcacccaa ggttcctgca ggtatgtcct
      121 cttcaaaggc aaaatctgaa attgccttta caagctcctc atcatactca actttgaagg
      181 aaacttctct tgctccaaaa tgtgaaaaac tccccgccgc actctttccc ggagaaatgt
      241 gaatatagtc ggagtaaacc gttgaatgag gtgttgaaat gattatggtt tcaggctgga
      301 tttcttttat catccttgcc acactcatga atgcatcaat cgtattctga atttcccttt
      361 ctcttccctt ccctacctca tgtattgcaa ttggtggatg gggtacaata aaaccgtagt
      421 ttatagccat aaactcacct ctttacctta ctacccctat tatatcctac ggtccataaa
      481 aatacaaaac atataataag ggagcgacaa agccgctcct tgtaaccttt aactgttgaa
      541 tgcacccaat atgaacaggt aatgatttgc ctctctcaaa atatgatctg ccaaaagcgg
      601 cattatcatc gacctcagct tgcagtccag gacaaggttc gtagcagtat tcttgaactg
      661 cttaaccttc ttcgttgcat caaggctctc tttagtaaac gaattgatat taggccttga
      721 ttccttgcat acttttttat tcagattgtc aaacaattta gccagatttc ttgcgtcgca
      781 tataaggggt tcttcggtag gatcaagaag accagcaaca aactccatat gctcttccat
      841 tctttcattc caaaagattt cttgtcccaa caaatcgcga aaatgcatgc ctctaagaag
      901 cgtatcaagt ctctttgcaa aaagccttgc ctctttggtt atgtggtcga ttaacagcgg
      961 atatgtagcc atgaacatgc tgcagtcaag cactctgttt agaagcatct ccttgaactc
     1021 aatgaactca agagtaagtt tatacgccct cttgttcaaa ctggtcactg cctgctctga
     1081 caagggtctc ctcctgtcat tagggcttaa ggacaattcc tttcttgtga tactcatgtt
     1141 gaaggggatg catgtgaaac aacttgtagc ttcttctgcg tccatggtat gacatgtaac
     1201 aaactgttcc gacttgacag cttttctgct aaccctgccc ggtgccatat cggcagcttc
     1261 cgacaaaaga ctttcataag attctctgaa atattttgct ttatctatac acctatcttc
     1321 cttttgcata aaagcaatct ccaggaagaa tgcatgttct ttcatgatac gtaaaaagaa
     1381 caaattagtg tcaagcgaaa tcgtaatata ttcactcaac gaaagcatag aatcctcctt
     1441 tttcaattat tcgcatagaa aaatatgcta ctacaatata ttcaaaggag aaatcatttg
     1501 ttaaaaacag ttgaacgatg aagagatttg tgatataatg acgataaatt agaaacagga
     1561 gggaaaatca tgaaaaaagc atttttcttc atcgtattaa caatactaac actaagtatg
     1621 gttgcggtat acgcaagcaa tattcagatt acattggaca aacaggaata tgttgttggg
     1681 gaaactgtaa caattaccta ctcagatgct gatggcgatg ccaatgcatg gtttgccata
     1741 tacccacaag gagaagagcc ttaccagtca tatccgggaa cggaaaaagt atgggcttac
     1801 gcaaacagta attctgaaca agctggttca gaacctgtta cagaaggaac tgtaaccctg
     1861 agcactgaag gacttgctcc aggcgaatac agatgctcat actttgttga cattggttac
     1921 aatgttggtg ctaccgtcac tctaaagata gttgaggaaa gcacaaaccc ggaaacatcg
     1981 gacgccattg cgtacatacc ttttatgatt ggagcaatct tgtcactcat ggtcttgaag
     2041 cgtaaagctg taaacatata agcaaagagg aaaggacata tcaaaacaca aatatgtcct
     2101 ttttcctcac ccaattattc catatctctc tttcagtttc agaatccggt atacgctttc
     2161 atttactctt tcctcactta cttcacctct attaaacgca tcgagtagtg aattgtaggc
     2221 agcttttccc ttttcaatat tctgagcaca aagaataata tcgcatcctg cattaaaggc
     2281 catgacgctt gcctgtccta ttgaatactc atcactgatg gcattcatgc ccaaatcatc
     2341 ggtaatcaca acacctttgt aattcatttc gtttcttaga aggtctgtga caagtttttt
     2401 tgacagtgta cttggaaggg aatctacttc cttgacaaca atatgccctg tcattatcat
     2461 ctccacatta tttttaatag cttccataaa tggaacaagc tcaaacccat aaagatcatt
     2521 taaactcttc ttcaccacag gaagcccata atgcgaatcc acactggtat ctccatggcc
     2581 aggaaaatgt tttgctgtag gaataatttt attgtctctc aaccct
//
LOCUS       DAONVY010000334         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26605_length_2623_cov_3.679128, whole genome shotgun sequence.
ACCESSION   DAONVY010000334 DAONVY010000000
VERSION     DAONVY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26605_length_2623_cov_3.679128"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PLI11_08510"
     CDS             <1..1125
                     /locus_tag="PLI11_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HPZ52945.1"
                     /translation="QEYILSANKTEMTRLRKISAFKCTRDFSEIVKKYVKHVEKSLFV
                     PEDLYLDDELTEYIDKETLEELFYDEFRSEAVYERTALVAERVAEMRKIKSFEIKEHI
                     KEGLDSMITCHSVETLYYRMYQDRKFFDEVGVNLEKYAPKTLLWEDICAIGLMSALMY
                     KFEFEPTLFYLLADEAQDFAPVFLELIKRRFPHCNMLFVGDRKQLVFENTGNFVDDIR
                     DIIQRRPFRKYELTTNYRSTNEIIEFASHIINKKEEHSIRSGNVPEVVRTGKDKMNEE
                     VAKFVVDMYEKGYMNIAVLCKSMKSAKEVNLKLDAKYAVKILPVYLAKGLEFDCVAIW
                     NASKDEYYDKSDRQMLYTACTRAMHELRVFYQGEITPFLK"
     gene            1144..1998
                     /gene="whiA"
                     /locus_tag="PLI11_08515"
     CDS             1144..1998
                     /gene="whiA"
                     /locus_tag="PLI11_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPZ52946.1"
                     /translation="MSTFQMRVKDELYVLEVNTDCCIFCEYAAIRLTLSGNKIKTKSS
                     LYAERLGFLVKKYHNLELDLEKMKKSDVYTIILPHFSYKPEPMDECCMKAFVRGCYIT
                     SGRTNDIDKATHVEMSFRSSLPYEICKTILEYYKLDIKTMMRKERYVIYMKHSETASD
                     FLTLIGAYKAVMEFENALIIREIRNNTNRAVNCDNANLQKASDAAYRQIQAIRLLEEK
                     NMLGTLPKHLLDTAMARIENPELDLKELGKLLNPPISKAGTAHRLNRIIEIANKIDEK
                     DMWFTKKT"
     gene            2120..2464
                     /locus_tag="PLI11_08520"
     CDS             2120..2464
                     /locus_tag="PLI11_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HPZ52947.1"
                     /translation="MELEMKRKGNLLILKVTGELDHHCADELRRRIDREILRTLVKDV
                     VFDFTELTFMDSAGIGVLMGRYRTVSHLGGTTWFLTEGKTSYFKHVMKILEMAGVFNY
                     IHKCKSLKECGV"
     gene            2467..>2623
                     /locus_tag="PLI11_08525"
     CDS             2467..>2623
                     /locus_tag="PLI11_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HPZ52948.1"
                     /translation="MDRTNYMRVVFPAKSENESLARILASSMAAQLDPTLEEISDLRT
                     AVSEAVTN"
BASE COUNT          864 a          464 c          619 g          676 t
ORIGIN      
        1 caggagtaca ttctttctgc caataaaacc gagatgacaa gactaagaaa aatctcagcc
       61 ttcaagtgca cgagagattt ttcagagatt gtcaagaagt atgtaaagca tgtggagaaa
      121 agtctttttg ttccggaaga cctttacctt gatgatgagc ttacagaata tattgacaag
      181 gagactttgg aggaactctt ctatgatgag ttcaggtcag aggcggttta tgaaagaaca
      241 gctctcgttg cggaaagagt agccgagatg agaaaaatca agtcctttga aatcaaggag
      301 catataaaag agggacttga cagcatgatc acctgccatt cagtggaaac cttgtattac
      361 aggatgtacc aggacaggaa gttttttgac gaggtgggag tcaatctcga aaagtatgca
      421 ccaaaaaccc ttctgtggga agatatatgt gcaatagggc ttatgagcgc ccttatgtac
      481 aagtttgagt ttgaacccac acttttttat cttttggcgg acgaagctca ggattttgcg
      541 cctgtatttc tcgaactaat taaacgcagg ttccctcact gcaacatgct gtttgtgggt
      601 gacaggaaac agcttgtatt tgaaaacacc ggcaactttg tggatgatat aagggacata
      661 atacaaagaa gaccctttag aaaatatgag cttacaacca attacaggtc gacaaatgag
      721 attatagagt ttgcctcgca cattataaac aaaaaagaag agcacagcat aagaagcggc
      781 aatgtaccgg aggtggttcg cacaggcaag gacaaaatga atgaggaagt tgcaaaattt
      841 gttgtcgata tgtatgaaaa gggatacatg aatattgcag tactctgcaa gtcaatgaag
      901 tctgcaaaag aggttaattt gaagcttgac gccaagtatg cggtgaaaat acttcctgtt
      961 tatcttgcaa aaggccttga gtttgactgt gttgccattt ggaatgcatc aaaggacgaa
     1021 tactacgaca agagcgaccg gcagatgctg tatacggcct gcacaagggc aatgcatgaa
     1081 cttagagtgt tctatcaagg tgagattaca ccgtttttga aataaaggaa agcaagtgat
     1141 cacatgtcta cttttcaaat gagggtgaag gatgaattgt atgtcttgga agtaaacaca
     1201 gactgctgca tattctgcga atatgcggct atccgtttga ctctttcagg aaacaagata
     1261 aaaacaaaaa gttctttgta tgcggaaaga ctaggatttc ttgtaaagaa gtatcacaat
     1321 cttgagcttg atttggagaa aatgaagaag agtgatgtgt acacaatcat cctgccacat
     1381 ttttcataca agcctgagcc catggatgag tgctgcatga aagcttttgt acgaggctgc
     1441 tacataacct cgggacggac caatgatatt gacaaggcta cccatgtgga gatgagtttc
     1501 aggtcaagtc ttccatacga aatctgcaaa accattcttg aatactacaa actggatata
     1561 aagacaatga tgcggaaaga aagatacgtc atatatatga agcattctga aaccgcatcg
     1621 gattttctta ccctgattgg cgcttacaag gcggttatgg agtttgaaaa tgcgcttatc
     1681 ataagggaga taaggaacaa cacaaacagg gcggtaaact gcgacaatgc aaatctgcaa
     1741 aaggccagtg atgcagctta cagacagata caggccataa ggcttttgga ggagaaaaac
     1801 atgcttggca cactgccaaa gcatctgctt gatactgcca tggcaaggat tgagaatccc
     1861 gagcttgatt tgaaggagct gggcaagctt ctaaacccac caataagcaa ggcgggcact
     1921 gcccacaggc tgaacagaat aatcgaaatt gcaaacaaaa ttgatgaaaa agacatgtgg
     1981 tttactaaga aaacatgatc gttttcaagg ctgcaccctt ttgtattcga cgggtctttt
     2041 tcaaattatt tcccaaaaca gggtaatatt ttgggaaata atatagaata tatgtacaga
     2101 atatcaagga gggtgagcca tggaacttga aatgaaaaga aaaggcaatt tactaatatt
     2161 gaaagtaact ggtgaacttg atcatcattg tgcggacgag ctcagacgga ggattgatag
     2221 ggaaatacta agaactctag tcaaggatgt ggtattcgat tttacagaac tgacatttat
     2281 ggacagtgca ggcattggcg ttctgatggg caggtatcgt acagtctccc atttgggagg
     2341 caccacctgg tttctaactg aaggcaagac aagctatttc aaacatgtca tgaaaattct
     2401 tgagatggct ggtgtgttca attatataca taaatgcaaa tctttgaagg aatgtggggt
     2461 atgacaatgg atagaacaaa ttacatgaga gttgtttttc ctgctaaaag tgaaaacgag
     2521 tctttggcga gaatactagc ttcctctatg gctgcacaac tggatccaac ccttgaggaa
     2581 atctccgatt tgcgtaccgc ggtcagtgag gccgtaacca act
//
LOCUS       DAONVY010000335         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26607_length_2623_cov_3.107087, whole genome shotgun sequence.
ACCESSION   DAONVY010000335 DAONVY010000000
VERSION     DAONVY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26607_length_2623_cov_3.107087"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1015
                     /locus_tag="PLI11_08530"
     CDS             26..1015
                     /locus_tag="PLI11_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPZ52949.1"
                     /translation="MTIASISISDIITQTLILFLLIGLGYLFAKTKYLPESSGVVLSK
                     LVIKVAMPATILVKMLGADFTREDYINGIYIYVFAIIFLLLTLGISLFASRVMKLSDK
                     TEGVYAIQAMFGNVAFFALPLFLVLFGEEGVIYAMFFNMGNDTLLWSVGIFMVNRHKE
                     RDIKKNLKHMINANTIAFVLGILLMVTGLNQVLLDSKYFFSVYSTIDYVASITTPMSM
                     IFIGFILAGIKLNVGDFKKKVPILVLSFQRLLLVPFLSMAVFALLDGFGLISESVVLI
                     TVLQLAMPIGTLTASLAMEYESDYFFATESVFISTLLSILTLPLISVLLKIIF"
     gene            1116..2060
                     /locus_tag="PLI11_08535"
     CDS             1116..2060
                     /locus_tag="PLI11_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPZ52950.1"
                     /translation="MKIKGFVKILIVFTVITLALCMLVSCAPNKKTQQDSGAFFWEVT
                     DGDGKKLYLLGSIHVADDDIYPFSDVIVNAFNESSALAVEVDIDALEKDVQMQIEVLQ
                     YFMYDNGSTIEDHIDEELFERAKQYLVERDMYSETYLLLKPIFWAELLSQTAAEKTGL
                     SFEKGVDMYFLKAAKKAKKDILEVESAVDQASAMATLSDNLQAFYLEDTLNNVGNLVK
                     ETLDLYELWKEADVEKLEKYVYNEDKKKALSDEQLEVFNEFEKVMIVDRNKHMVEKAV
                     EYINSGKTVFYVVGAAHMVGDSGLVNQLEMKGYKVAKR"
     gene            2088..>2623
                     /locus_tag="PLI11_08540"
     CDS             2088..>2623
                     /locus_tag="PLI11_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52951.1"
                     /translation="MRRMRIVILISVILTINCMLVVSAEDRPMIVLDNETYADVFGNV
                     SHVSELYYENGAIKVTFTPGGNDPYIYLPLSLMGDIPCNEYKVLAIKMKAPQPGSGNL
                     YFATDRHNSLDENKNVRTSEYRADGKWQIVTVDFSTNEYYEGVVTQFRFDVYPKSVDG
                     VLEIEWIAMFRSLEEVKEV"
BASE COUNT          790 a          421 c          619 g          793 t
ORIGIN      
        1 aaagtatcaa gattgagttt ctaaaatgac aattgcaagt attagcatat cggatataat
       61 tacgcagacg ctgattctgt tcttattaat aggtttggga tacctgtttg caaaaacaaa
      121 atacctgccc gagtccagcg gggtggtatt gtccaaactg gttataaaag ttgccatgcc
      181 cgccacgatt ctagttaaga tgcttggggc ggactttacc agagaggatt acataaacgg
      241 aatatacatt tatgtttttg caatcatatt ccttttgttg acgcttggca tttcgttgtt
      301 tgcatcaaga gtcatgaagt tgtcagataa aactgaaggt gtctatgcaa tccaggccat
      361 gtttggaaac gtggcgttct ttgcacttcc gcttttcctg gttttgttcg gcgaggaagg
      421 agttatttat gccatgtttt tcaacatggg aaatgacacc cttctttggt ccgtaggcat
      481 ctttatggtt aacagacaca aggaaagaga tatcaagaag aacctaaagc acatgataaa
      541 cgccaacaca atagcctttg ttctcggaat tctgcttatg gtgacaggtt taaatcaggt
      601 gctgcttgat tcaaaatact tcttcagcgt gtacagtacc atagattatg tggcaagcat
      661 tacaactcca atgtcgatga tattcatagg attcatactg gcaggtataa agctgaatgt
      721 gggtgatttt aagaagaagg ttcctattct tgttttgtct tttcagagac tgttgttggt
      781 tccgttttta tccatggcgg tgtttgcact gcttgacggt tttggcttaa tatcggaatc
      841 tgttgtattg ataactgttt tgcagctggc aatgcctatt ggtacattaa ccgcatctct
      901 tgccatggaa tatgaaagcg attatttctt tgccacggaa tcggtattca tatcaacttt
      961 gttaagtatt ctaacccttc cattgatatc agtattgcta aaaattatat tttaatagca
     1021 aaaccggtaa tgaaaatatt aatcattacc ggttttcttg ttgaaataat taacagaacc
     1081 gatataatat agatataaag atttgggagg aagtcatgaa aattaaaggt tttgtcaaaa
     1141 ttctaattgt tttcacagtc ataacacttg ctttatgcat gttggtatcc tgtgctccca
     1201 acaaaaaaac tcagcaagat tcgggagctt ttttctggga agtgacggat ggtgatggca
     1261 agaagttgta tctgttgggc tcgattcatg ttgctgatga tgatatctat ccttttagcg
     1321 atgtgattgt gaatgcgttc aatgaaagct ctgcattggc agtggaggtg gatattgacg
     1381 ctcttgaaaa ggatgtgcaa atgcaaatag aggttttgca atatttcatg tatgacaatg
     1441 gttccacaat agaagaccat attgatgaag agctgttcga aagagcaaaa cagtatcttg
     1501 tggaacgaga tatgtacagt gaaacctacc tgcttttgaa accaatattc tgggctgagc
     1561 tgctaagcca gactgctgct gaaaaaacag gactctcttt tgaaaagggt gttgacatgt
     1621 atttcttgaa agcggcaaag aaggccaaga aggatattct ggaagttgag tctgctgttg
     1681 atcaggcaag tgccatggca accctcagcg acaatctgca ggcgttttac ctagaggata
     1741 cgctgaacaa tgtcggcaat ctggtaaagg agaccttgga tttgtatgag ctttggaagg
     1801 aagctgatgt agagaagctt gagaaatatg tttacaacga ggataagaaa aaggccttgt
     1861 cggatgaaca gttggaggta ttcaatgagt ttgagaaggt catgattgtt gacagaaaca
     1921 aacacatggt tgagaaagct gtagaataca taaatagcgg aaaaacagtc ttttatgtag
     1981 ttggtgcagc tcacatggta ggtgatagcg gactggttaa tcaattggag atgaagggat
     2041 acaaggtagc caaaagataa atctgaaagg gaattgatta aagtaatatg agaagaatga
     2101 gaattgttat tctaatatcg gttatactga caataaactg catgttggtt gtatccgcag
     2161 aggatcgtcc catgattgtt cttgataatg aaacttatgc tgatgtattt gggaatgtaa
     2221 gccacgttag cgaactgtat tatgaaaatg gtgctataaa ggttaccttt acaccaggag
     2281 gaaacgaccc ttacatatat ttgcctttat cgctgatggg cgatatacct tgtaacgagt
     2341 acaaggtttt ggccataaag atgaaagcac cccagcctgg ttcaggcaac ctttattttg
     2401 caactgacag gcataacagt ttggatgaga acaagaatgt acgtacatcg gagtacagag
     2461 cggatggcaa atggcagata gtaacagtgg atttcagcac caacgagtat tatgaaggcg
     2521 tagttaccca gtttaggttc gacgtctatc cgaaaagtgt ggatggtgtt ttggaaatag
     2581 aatggattgc catgtttcgt tcactggagg aggtaaagga agt
//
LOCUS       DAONVY010000336         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26651_length_2620_cov_2.497856, whole genome shotgun sequence.
ACCESSION   DAONVY010000336 DAONVY010000000
VERSION     DAONVY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26651_length_2620_cov_2.497856"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLI11_08545"
     CDS             <1..733
                     /locus_tag="PLI11_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HPZ52952.1"
                     /translation="WVYCGAGRSYVIGRMEKSNGIFEFVPETDLITPMHGCDDMYAAQ
                     TFYNAPDRRIGIYWLIDKTSEQIPGKPYDGALSFPVEYKLINNRLCIYPVKELESLRK
                     GVLFETRNAKSNHFSIDIEGKLLDIEVHFKELSQRFGFKLITEVSSLDIVYDRDIMKL
                     IIDKNHVSSIAKGRNEIDLKPDKEGSIRLRIMLDTSILDVFGNHGEASYMSFIYPDKP
                     IKELVFFGENGISVDYLTVYEIGRD"
     gene            734..>2620
                     /locus_tag="PLI11_08550"
     CDS             734..>2620
                     /locus_tag="PLI11_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HPZ52953.1"
                     /translation="MKRVFVTLIVLMFVLIHIIYVFGDGGSPFMKIEIVGIDYEFADK
                     QSEYFVNVDNKTSSIAIKAKVKEGFRLYINNEEVENDSEINIDLNVGYNLVLLTAKSP
                     EGDKINKRVAVRRMQDENILYKEKYRPQFHNSTQINLMNDPNGLVYNDYTKEYHLFYQ
                     QDNAFEKGVETKSWAHAVSKDLVHWKDLGLAIMPDDLGEIFSGSCVIDRYNTSGLFPD
                     DVPEGSRMVALYTSYAGRIGDSYYGIEKQGLAYSLDHGVTWIKCDKNPMIKNGENHKQ
                     VFTTGFRDPKVIWYEDSSYENNGIWIMVVAGGQARIYTSTDLVNWTYNGVCTYSNGRR
                     PIEAECPDLFPMPVNGDENNVKWVFCGSDFNGGDSSIFYIVGDLIKDDEGNFKFVAET
                     EKSESINANNEVYAAQTFFNTDRRISISWMRDWVYFNEEDNIKNWLGTHTLPVELKLV
                     TDSKGNYRLKYTLVEEVELLRDKLLFSIEDKKVGSEDGNLLRELNAKLYDIELVVDIG
                     TAQEFGFDLRSKDSEKTTVKYVVKDNKLILDRSKSGKGVSNPVYMMDVKPMEGNLVKL
                     QILVDSSIIDVYANDGAAMCNTQIYPGDESTDMNFFVSGGEVTIKSMKIFGMKSIWEN
                     WQEPY"
BASE COUNT          859 a          382 c          625 g          754 t
ORIGIN      
        1 gtgggtgtat tgtggagcgg gcagatccta tgtaattggc cgaatggaga agagtaatgg
       61 gatttttgag tttgttcctg aaacggattt gataacacct atgcatggat gcgatgacat
      121 gtatgcagct cagacctttt acaatgctcc agacagaaga ataggcatat actggcttat
      181 tgacaaaacc tcggagcaaa tacctggaaa accttatgac ggtgctcttt catttcctgt
      241 tgaatacaaa ctgataaaca acaggttgtg catataccca gtaaaggaat tggagtcatt
      301 gagaaaagga gtattgtttg agacaaggaa tgcaaaaagt aatcattttt ctattgatat
      361 tgaaggaaag ctgcttgata ttgaagttca tttcaaggaa ctgtctcaaa ggtttggttt
      421 caaactgata acggaagtca gtagcttaga cattgtttat gacagggata taatgaagct
      481 cattattgac aaaaatcatg tcagcagcat tgcaaaagga agaaatgaaa ttgatttaaa
      541 acctgataaa gaaggaagca ttcggttaag aatcatgttg gatacatcaa tccttgatgt
      601 ctttggcaat catggtgaag caagttacat gtctttcata tatcctgaca aaccaataaa
      661 ggaacttgtt ttctttggtg agaacgggat tagtgttgac tatctgactg tatatgagat
      721 tgggagggat tgagtgaaaa gagtatttgt aactttaatt gtacttatgt ttgttttgat
      781 tcatattatt tatgtttttg gggatggagg tagtcctttt atgaagattg agatagttgg
      841 cattgattat gaatttgcag acaagcagag cgagtatttt gtaaatgttg ataacaaaac
      901 ttccagtata gcaatcaaag ccaaagttaa ggaaggattt agactgtaca taaacaatga
      961 ggaagttgaa aacgatagtg aaataaatat tgatcttaat gtaggctaca atcttgttct
     1021 acttactgct aaaagtccgg aaggtgacaa gataaacaaa agggtggcag tcagaagaat
     1081 gcaggatgaa aacattttgt ataaagagaa gtacagacca caatttcaca actcaacgca
     1141 aatcaacctt atgaatgacc ctaacggtct tgtgtacaat gactacacta aagaatatca
     1201 ccttttttat cagcaggata atgcttttga aaagggagtt gagacaaaaa gctgggcgca
     1261 tgccgtatca aaagatttgg ttcattggaa ggatttgggg cttgcaataa tgcctgatga
     1321 cttgggagag attttttcag gctcgtgtgt cattgacagg tacaacacat caggactgtt
     1381 tcctgatgat gtgcctgagg gaagcagaat ggttgctctt tatacatcct acgcaggcag
     1441 gattggcgac agctactatg gtattgaaaa gcaggggctt gcttattctc ttgaccatgg
     1501 cgttacatgg attaagtgtg acaagaaccc gatgattaaa aatggtgaga atcacaagca
     1561 agtgttcaca acaggattca gagatccaaa ggttatatgg tacgaggatt catcttatga
     1621 gaacaatggt atctggatta tggtggtggc aggaggtcag gcaagaattt acacatcaac
     1681 cgaccttgtc aactggacat ataacggggt gtgcacatat tcaaatggaa gacgtcctat
     1741 tgaagctgag tgtcctgatt tgttcccgat gcctgttaat ggtgatgaga acaatgttaa
     1801 atgggtgttc tgcggcagcg acttcaatgg tggtgacagc agtatctttt atattgttgg
     1861 agatttgatt aaggatgatg aaggcaattt taagtttgtt gctgaaaccg agaagtcgga
     1921 atcaataaat gccaataatg aagtgtatgc tgcacaaacc ttctttaata cagatagacg
     1981 tatcagtatc agctggatga gggactgggt ttattttaat gaagaagaca acattaaaaa
     2041 ctggcttgga acacataccc ttcctgttga attgaaactg gttactgaca gtaaaggtaa
     2101 ctacagactt aaatacacat tggtggagga agttgagctg ttaagagaca aattgttatt
     2161 ctctatcgaa gataaaaagg ttggctcaga ggatgggaac ttgttgagag aattgaatgc
     2221 aaagctttac gatattgaac ttgtagttga tattgggact gcacaggagt ttggttttga
     2281 tttaagaagc aaagactctg agaaaaccac ggtgaagtat gtagtcaagg acaacaagct
     2341 gatacttgac agaagcaagt caggaaaagg cgtttcaaat cctgtttata tgatggatgt
     2401 aaagcctatg gagggtaacc tggtaaaact tcaaatcctt gtagactcat cgattattga
     2461 tgtatatgcg aatgatggcg ctgcaatgtg caacacacaa atatatccag gcgatgaatc
     2521 tacagacatg aacttctttg tttcaggtgg agaggttaca ataaagagca tgaagatatt
     2581 tggtatgaag tcaatatggg aaaattggca ggaaccttat
//
LOCUS       DAONVY010000337         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26737_length_2613_cov_2.463643, whole genome shotgun sequence.
ACCESSION   DAONVY010000337 DAONVY010000000
VERSION     DAONVY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26737_length_2613_cov_2.463643"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /gene="rimP"
                     /locus_tag="PLI11_08555"
     CDS             complement(<1..407)
                     /gene="rimP"
                     /locus_tag="PLI11_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPZ52954.1"
                     /translation="MSKIAATVKNLVESEIEKMNIDLIDVEYVKEGSRKYLRVYIDKR
                     GGVTIDDCEMVAKAIGPMIDELDPIQESYVFEVSSPGIDRPLKTEKDFKRYEGHLVEV
                     RLYTQVEGRKTYEGYLDGLYGDVLKITDEKSNVL"
     gene            complement(537..794)
                     /locus_tag="PLI11_08560"
     CDS             complement(537..794)
                     /locus_tag="PLI11_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HPZ52955.1"
                     /translation="MGRVSSFQKKEVINLSDGRRLGYVCDCDVSFETGRIEAIVVGGT
                     TKLFGGLGKDNELVIPFSKIKRIGEDIIIVDIEERFLKRYN"
     gene            complement(842..1618)
                     /gene="sigG"
                     /locus_tag="PLI11_08565"
     CDS             complement(842..1618)
                     /gene="sigG"
                     /locus_tag="PLI11_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HPZ52956.1"
                     /translation="MIINKVEICGVNTSKLPILSNQQKKELFERILQGDEKAREEFIK
                     GNLRLVLSVIQRFNNKGEQVDDLFQVGCIGLIKAIDNFDVTQNVQFSTYAVPMIIGEI
                     RRYLRDNNAIRVSRSLRDTAYKALHVRERLFNESNREPTISEIAKDMDISREELVMAM
                     DSIQDTISLYDPIFQDGTESICVLDQIKDDNVSNHVWEQNMTLSNALDRLGKRERLII
                     DMRFFEGRTQMEVAEEIGISQAQVSRLEKNALKSMRKYVV"
     gene            complement(1745..2497)
                     /gene="sigE"
                     /locus_tag="PLI11_08570"
     CDS             complement(1745..2497)
                     /gene="sigE"
                     /locus_tag="PLI11_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HPZ52957.1"
                     /translation="MMDFNKLKVKGFVIKYRLFVIRNKIRRFLGLGSSMPIFYVGGPD
                     MLPPPLSNEEESYLLAHFDDKDMDVRTILIEHNLRLVVYIAKKFENTNVNIEDLVSIG
                     TIGLIKAINTFAPEKNIKLATYASRCIENEILMYLRRSNRQKLEISIDEPLKVDWDGN
                     ELLLSDILPGDVEGVHKKIDEEVDHQLLKEALKKLSVREKRIMDLRFGLTGGEERTQK
                     EVADMLGISQSYISRLEKRIINRLRKEILKVS"
BASE COUNT          689 a          662 c          381 g          881 t
ORIGIN      
        1 agcacgtttg acttctcatc tgtgattttc aacacgtctc cgtaaagtcc gtcaagataa
       61 ccttcatatg tctttctacc ttccacctgc gtgtataacc tgacctcaac caaatgacct
      121 tcatatcgct tgaagtcctt ttccgtcttc agcggcctgt caataccagg tgaagatact
      181 tcaaacacat atgattcctg tattgggtca agttcgtcga tcatagggcc gattgccttg
      241 gcaaccattt cacagtcgtc aatcgtcact ccgccccttt tgtcgatata taccctcagg
      301 tattttctag aaccttcctt tacatattct acatcaatca aatctatgtt cattttctca
      361 atttcagact caactagatt tttaactgtt gctgcaattt tcgacatctt acgcacctac
      421 tttaaaattt aaaatgaaag agtgggacct cccactcttt cgtcactgtt gatatagcaa
      481 ctatacaact agtattatac acattgtatc aaataaagtc aatgataatt ttgacgtcag
      541 ttataccgtt ttaagaacct ttcctcaata tcaacaataa ttatatcttc gcctatcctt
      601 tttattttgc taaacggaat aactagctca ttatcctttc caagtccgcc aaaaagcttt
      661 gtggttccgc ccaccacaat agcttcaatt ctgcctgtct caaaactgac atcacaatcg
      721 cagacataac caagtcttct gccatcggat aaattgatta cctctttctt ttgaaatgag
      781 ctgaccctgc ccacaacaat ccctccgtat gcttgaggca caactcatcc tttggcttta
      841 ctcacactac gtatttcctc atactcttca atgcattttt ttcaagcctg gatacttgtg
      901 cctgacttat tcctatttcc tccgcaacct ccatctgggt cctgccttcg aagaacctca
      961 tatcgattat gagcctttct ctttttccaa gtctgtcaag cgcattactc aaagtcatgt
     1021 tctgctccca aacatggttg ctgacattgt catccttaat ctgatccagc acgcatatgg
     1081 attccgttcc atcctggaat ataggatcat acaaggagat tgtgtcctga atggaatcca
     1141 tcgccattac cagttcctct cttgatatat ccatatcctt cgctatttca gatatggtag
     1201 gttccctgtt gctctcatta aacaatcttt cccttacatg cagggccttg tatgcggtat
     1261 cccttaacga ccttgatacc cttatcgcgt tattatccct aagatacctg cgtatttccc
     1321 caattatcat aggtaccgca tacgttgaga actgtacatt ctgcgtcaca tcaaaattat
     1381 ctatagcttt aatcaggccg atacatccga cctggaacaa atcatctacc tgttcacctt
     1441 tgttgttgaa gcgctgaatc acacttaaaa ctagtctgag gttacctttt ataaattctt
     1501 cccttgcctt ttcatcgcct tgaagaatcc tttcgaaaag ttccttcttt tgctgattgc
     1561 tcaaaatggg aagttttgac gtatttactc cacatatttc aaccttgtta attatcatga
     1621 cgaacctccg aatacttagt ttgacacggg aacatttttg ccccttcttt tttagattat
     1681 tgccatcgga agttctttta atacggaaac tgattgttaa acatttactc aaaaaattat
     1741 gtacttatga cacttttaat atctcttttc taagtctgtt aattattctt ttttccagtc
     1801 tggaaatata tgactgagaa ataccaagca tgtcggcaac ctctttctgc gttctttcct
     1861 caccacctgt aagtccaaat cttaaatcca taatcctttt ttccctgaca ctgagttttt
     1921 taagcgcttc cttcaaaagc tgatggtcca cttcctcgtc aattttcttg tgtacaccct
     1981 ccacgtcacc agggagaata tcagacaata aaagctcgtt tccatcccag tccaccttca
     2041 aaggttcgtc aatcgatatc tcaagtttct gtctgttgct ccttcttaaa tacataagta
     2101 tctcattttc tatgcatctt gacgcataag ttgcaagttt gatgtttttt tcaggcgcaa
     2161 acgtattgat tgccttaatc aaaccgatcg tgcctattga aacaagatcc tcaatgttca
     2221 catttgtgtt ctcaaatttt tttgcaatgt ataccacaag tctgagattg tgctcaataa
     2281 gtatggtcct tacatccatg tccttgtcgt cgaaatgcgc cagaagatag ctttcctcct
     2341 cgtttgacaa aggaggcgga agcatatccg gccctcccac atagaatatg ggcatagagc
     2401 tgccaagtcc caaaaaacgc cttatcttgt ttcttatcac aaacaacctg tacttgatta
     2461 caaaaccttt caccttaagc ttgttaaagt ccatcatgcc tattctcccc ctcttttgta
     2521 tatcaggcta ccaattccgg agccaaaagc gcactgtatt tgttgtcttc cgataactgt
     2581 ttgtcataaa ggcaaacaat cacatcccta aca
//
LOCUS       DAONVY010000338         2604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26864_length_2604_cov_2.831306, whole genome shotgun sequence.
ACCESSION   DAONVY010000338 DAONVY010000000
VERSION     DAONVY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26864_length_2604_cov_2.831306"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLI11_08575"
     CDS             <1..510
                     /locus_tag="PLI11_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52958.1"
                     /translation="AIISKEDYDFILENVIRINYTFFTDKVQNASLKAYEVNKDDLVK
                     SVEMFFDLKESKRKIALSALSTIAAIVFLTYFEFSFARRKNRKDIETFMKLGMPAKNV
                     RIIYFLIVYTSFAITVLLTLLISKFIYLDRMVGIYCDPVVLLTFFAVLLITGSISILV
                     QSLFIKRYC"
     gene            complement(551..1120)
                     /locus_tag="PLI11_08580"
     CDS             complement(551..1120)
                     /locus_tag="PLI11_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52959.1"
                     /translation="MRKTVLLLIFLLVLLSGCLKQENTMHENTPDTYISPYNGEVSRG
                     YVIEDMVDSWEYIELIHRNFYDIYTVNTQFEISRFELIYRVEDYPNPDCHMFNKKGYT
                     YWILYNEYKLQLSYVTDDGTIYEDVVEYNGKEYNYFEWDNNRSIIVKRVNENTYLALT
                     CDVNEKFDPSLLEMELFDKMFVAQTPPPH"
     gene            complement(1159..1851)
                     /locus_tag="PLI11_08585"
     CDS             complement(1159..1851)
                     /locus_tag="PLI11_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52960.1"
                     /translation="MNEMEYLKDKFESIKLTPEADERIQKGIKGKLGKGKKRKVFASI
                     VAVFLLFFVLLFIFSDRQKDPGVILNEPTNTPNITVPNSSFTPEPTKTLNPAPTKRSM
                     TFFDMDSLFEEVKKDEEFYYEVKPLTDMMKLDDSRIGVIELLEDNRVVFYLENGLELD
                     LYYNTGYGNTTIESYHRSMEENKNYNMYKGSSFDYLMKNSLDEAIFLFENILVVVSGE
                     KAQEFVVTINAS"
     gene            complement(1844..2287)
                     /locus_tag="PLI11_08590"
     CDS             complement(1844..2287)
                     /locus_tag="PLI11_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPZ52961.1"
                     /translation="MYETYKNLVYRISVIYLGNTFDAEDLVQNVFVSYYKRLPKFESQ
                     EHARNWFARVTVNMCINFRKSYWRRFVDKADEIDVPEDMNVERIEILDILSVLPDKSR
                     LIMLLYYYEQYTVGDIAGMMNMSESAIKMQLKRSREKLKEVLGDE"
     gene            complement(2391..>2604)
                     /locus_tag="PLI11_08595"
     CDS             complement(2391..>2604)
                     /locus_tag="PLI11_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPZ52962.1"
                     /translation="WGIALLASYMKNRQKDETLTGYLDGKVFAQDDSSVLEPDEKDVK
                     GFNVFLERYKRGLEIERAAVESLYGD"
BASE COUNT          809 a          568 c          376 g          851 t
ORIGIN      
        1 gctataatta gtaaggagga ttatgacttt attcttgaaa atgttatacg tataaactac
       61 accttcttta cagacaaagt ccaaaatgca agtctaaagg cctacgaagt caataaagat
      121 gacttggtga agtccgttga gatgttcttt gacttgaaag aatcaaaaag gaaaatagcc
      181 ttgtctgcac tatccacaat cgcagccatt gtttttctca cctattttga gttcagtttt
      241 gccagaagaa agaacagaaa agacatagaa acttttatga agctaggaat gcctgcaaaa
      301 aacgttcgta taatctactt tttaatagtt tacacaagtt ttgcaataac agtacttctt
      361 acattgctta tcagcaagtt catttacctt gacagaatgg ttggcatata ttgcgatcct
      421 gttgtgctgc tgacattctt tgcagtcctt cttataacag gaagcatttc aatactggtc
      481 cagtcgttgt ttataaagcg gtattgctag tcagcaatac cgcaaatcat ccgtatcaaa
      541 gatcgtaagg ttaatgcggc gggggagttt gagctacaaa catcttgtca aaaagctcca
      601 tttcaagcaa ggaagggtca aacttttcat tgacatcaca agtcaatgcc aaatatgtgt
      661 tctcattcac ccgtttaaca atgatactcc tgttgttatc ccactcaaag taattgtatt
      721 cctttccatt gtattcaact acatcctcat atattgttcc atcatcggta acataggata
      781 actgtaactt gtattcatta tataaaatcc aatatgtgta tcctttcttg ttaaacatgt
      841 ggcaatcagg attggggtag tcctccactc tatatatcag ctcaaaccgt gatatctcaa
      901 actgtgtatt aacagtataa atatcgtaga aattacgatg tataagctct atgtattccc
      961 aggaatcaac catgtcctca atcacataac ctcttgaaac ctcaccatta tacggagata
     1021 tgtaagtgtc gggcgtattc tcatgcatgg tattttcctg ttttaagcaa ccggacagta
     1081 acacaagcag aaatattaat aacaaaacag tttttctcat acaactcctc cattagtcat
     1141 cttcataaat atatacaatt atgaagcatt gatggtcaca acaaactctt gtgctttttc
     1201 acctgaaaca actacaagaa tgttttcgaa tagaaagatg gcttcatcca atgaattttt
     1261 cattaagtaa tcaaaactac ttcctttata catattgtaa ttcttgttct cttccatact
     1321 cctatgatat gattcaatgg ttgtgttacc atatccggtg ttgtaataca gatctagctc
     1381 caaaccattc tctaaataga aaaccaccct attatcctca agcaactcta taacgccaat
     1441 tctggagtca tccagtttca tcatatcagt caaaggctta acctcgtaat aaaactcctc
     1501 atctttcttt acttcctcaa ataaagaatc catatcaaaa aaagtcatgc tccttttcgt
     1561 cggtgcagga ttaagtgttt tagttggctc tggagtaaaa gaactattag gcactgtaat
     1621 atttggtgta tttgttggct catttagaat tacaccagga tccttctgtc tgtcagaaaa
     1681 gataaaaaga agtacaaaaa aaagcaagaa gacagccaca atgcttgcaa aaacctttct
     1741 ttttttgcct ttccctagtt tcccctttat acctttttga attctttcgt ccgcttcagg
     1801 ggttaacttg atactctcaa acttatcctt taaatactcc atctcattca tctccaagaa
     1861 cctccttgag cttctccctg cttctcttca gttgcatttt aattgcggac tccgacatat
     1921 tcatcattcc ggcaatatca ccaactgtat actgttcgta ataatacagg agcataatca
     1981 acctgctctt atcaggcaaa acagacaata tatcaagtat ctcaattctt tccacattca
     2041 tatcttctgg tacatcaatt tcatctgctt tgtcgacaaa ccttctccaa taagattttc
     2101 tgaaatttat gcacatgttt actgtaaccc ttgcaaacca gttgcgtgca tgctcctgac
     2161 tttcaaattt gggaagtcgc ttgtaatatg atacaaaaac attctgcacc aagtcctccg
     2221 catcaaatgt gttgcccaaa tatatcacag atattctgta cacaagattt ttgtatgttt
     2281 catacatttc ttagtctttc tccttcatta ccatatacaa tccatctccc caaatggtca
     2341 cacatgagtt tggaatatta aaaatgctat tatatagtca ggtccaataa ttaatctcca
     2401 tacaaacttt caaccgcagc tctttctatc tcaagtcctc ttttgtacct ctccaaaaag
     2461 acattgaacc cttttacatc cttttcatca ggctccaata cagaagaatc atcctgtgca
     2521 aacactttgc catcaagata acctgtcaaa gtctcatctt tttgcctgtt cttcatgtaa
     2581 gaagcaagaa gagcaatacc ccag
//
LOCUS       DAONVY010000339         2600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26916_length_2600_cov_3.612181, whole genome shotgun sequence.
ACCESSION   DAONVY010000339 DAONVY010000000
VERSION     DAONVY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26916_length_2600_cov_3.612181"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PLI11_08600"
     CDS             <1..1594
                     /locus_tag="PLI11_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPZ52963.1"
                     /translation="CRQVPTKPARSFHEAVQSLWFAHIINTWEDGINANSLGRLDQIL
                     YPYYIHDIENGTITKEQVFELICCLWIKLYRDYDVQQSCVGGCLADGSSVVNDLSYMM
                     LDATEQLGFVRCLSVRFSSQTDKMFLRRALEVVGRVKSGIPFFFNDEVMIPALVSKGI
                     SLEDARNYTQIGCVETVIPGKSNPHAVSGQTNLLKAVEYAFGNGRSLFNPEFKPGLTT
                     GDIDTFKTYEDFENAVLKQIKHILDNSCKKVYEWTCAARKNAVKPVKSLLTEGCVESN
                     RDFNNHGAKYDYYQIMLGGVPNLADSLMVVKKLVFEDERYSLQYMKEQLEKDFPNEIF
                     RMECIHKVPKYGNDIDEVDEIASRIIEYSCKVLDEMEEKYGLKFHAQPFTFLWMVDHG
                     RVTSATPDGRRKGEIIAYSMSPMQGRDKNGFTALMNTLSKLPGKMCPGTVSAIVEVDP
                     KLYTENNIDYFVDILVACAGKGIANVQFNTIDADTLIDAQKYPDKYSNLAVRVSGFSQ
                     KFNLLDKNLQDHIIGRTKHQCM"
     gene            1585..2493
                     /locus_tag="PLI11_08605"
     CDS             1585..2493
                     /locus_tag="PLI11_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPZ52964.1"
                     /translation="MHVDTLIANIVRGSLHDGRGIRTVVYFKGCNMKCKWCHNPECIS
                     FQSEILYADSKCIRCGVCVETCTNHVIYDDKHVFNREGCTACGECASNCPVNALELCG
                     VNMSTDTVLKEILKDKHYYDTSSGGVTFSGGECLLHPEYVLELCKLCKENAVSITIET
                     ALNVSWEAIELVYPYVDTFYVDIKHMDDEKHCFHTGCSNERVLNNLKRLAKVHGDIIV
                     RTPLIPRVNDSYENLVETAKFAHNLGIKRVEFLRYNILGKSKYDLLQKDYVSYSDETQ
                     AKEYMDDLCQRINCLIGEGDFVFWVE"
BASE COUNT          836 a          417 c          597 g          750 t
ORIGIN      
        1 atgcagacag gtacctacaa aacctgcacg ttctttccat gaagcagtac agagtttatg
       61 gtttgcccat ataatcaata cctgggaaga cggaataaat gcaaattcgt tagggagact
      121 ggatcagatt ctttacccat attacataca cgatatagaa aatggaacta ttacaaaaga
      181 acaggttttt gaacttatct gctgcttatg gataaaatta tacagagatt acgatgtgca
      241 gcaatcttgc gttggtggat gtctggctga tggcagttct gtcgtaaatg atttgtcata
      301 catgatgctg gatgcaacgg agcagcttgg atttgttcgc tgtctgtctg taaggttctc
      361 atcacaaact gacaaaatgt ttttaagacg ggctctcgag gtggtcggaa gagtcaaatc
      421 tggaatacct ttctttttca atgatgaggt aatgattcct gcccttgttt caaagggaat
      481 ctcacttgaa gatgcaagaa actatacaca gattggttgt gtggaaactg taattcccgg
      541 aaagtccaat cctcatgcag tttcaggtca aacaaatctt cttaaagctg ttgaatatgc
      601 ttttggaaac ggcaggagtt tgtttaatcc tgaattcaaa cctggactga caactggaga
      661 tattgataca tttaaaactt atgaggattt tgaaaatgca gttttgaagc aaatcaaaca
      721 catattggac aattcctgca aaaaggtgta tgaatggaca tgtgctgcaa gaaaaaacgc
      781 agtaaaacct gtaaagtcac ttcttaccga agggtgtgtt gaatccaaca gggatttcaa
      841 caaccatggt gcaaagtacg attattatca aatcatgctg ggaggcgtac caaacctggc
      901 ggattctttg atggttgtaa agaaactagt ttttgaagat gaaagatatt cattacagta
      961 catgaaagaa cagctcgaaa aggactttcc aaatgaaatc ttcaggatgg agtgtattca
     1021 caaggtacca aagtatggta atgatattga tgaagtggat gagattgcat cacgaattat
     1081 tgaatattcg tgtaaagttc tagacgagat ggaagagaaa tatggtctaa aatttcatgc
     1141 acagcccttt acttttcttt ggatggtcga ccatggcaga gtaacttcag caactcctga
     1201 cggacgaaga aagggagaaa taattgcata cagcatgtca ccaatgcagg gacgtgacaa
     1261 aaacggcttt accgcactta tgaacacatt gtcaaaattg cctggaaaaa tgtgtcctgg
     1321 cacggtttct gccattgttg aagtcgaccc aaaactgtat acggagaaca acatagatta
     1381 ctttgtagat atacttgttg cgtgtgccgg taaaggcatt gcaaatgttc agttcaacac
     1441 catagatgca gataccttga tagatgcgca aaagtatcct gacaagtatt caaatcttgc
     1501 agtacgagta tccggcttca gccagaagtt taatttgctg gacaaaaact tgcaggatca
     1561 tattatagga aggacaaagc accaatgcat gtagatacat tgattgcaaa tattgtaaga
     1621 ggatctctac atgacggcag aggcattcgc acggtagtat atttcaaagg ctgcaacatg
     1681 aaatgcaaat ggtgtcacaa cccggaatgt atttcttttc agtctgagat attgtatgca
     1741 gatagcaaat gcataaggtg tggtgtatgt gttgagacat gtacaaatca tgttatttat
     1801 gatgacaagc atgtttttaa tcgtgagggt tgtacagcct gtggggaatg tgcctccaat
     1861 tgtcctgtga atgctctgga actatgcgga gtaaatatga gtactgacac agtcctaaag
     1921 gagattctga aggacaaaca ctattacgat acatcgtcag gaggagttac attctcaggt
     1981 ggggagtgcc tgcttcatcc agagtatgta cttgaattgt gcaagctttg taaagagaat
     2041 gcagtttcga taacaataga gactgcattg aatgtgtctt gggaagcgat tgagctggtt
     2101 tatccgtatg tggatacttt ttatgtggat attaagcata tggatgatga aaagcactgt
     2161 ttccatactg gttgtagtaa tgaaagggta ttgaataatc tgaaaaggct ggcaaaagtc
     2221 catggcgata tcattgtaag aactccttta ataccgagag tgaatgattc gtatgagaat
     2281 cttgttgaaa ctgctaaatt tgcacacaac ctaggaataa agagagttga gtttctgaga
     2341 tacaacatac tgggtaaatc gaaatatgat ttgctgcaga aagattatgt atcatattct
     2401 gacgaaacac aagccaagga gtatatggat gatttgtgcc aaagaattaa ttgcttgatt
     2461 ggtgaggggg attttgtttt ttgggtggaa tagcaaactg ataaaagggg gagtttcaaa
     2521 tgattaagat aagtcctgta cctgatgaaa gttacaagtc ccatgatttt gtaattactg
     2581 taaatggaat caatttggat
//
LOCUS       DAONVY010000340         2600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26919_length_2600_cov_3.273870, whole genome shotgun sequence.
ACCESSION   DAONVY010000340 DAONVY010000000
VERSION     DAONVY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26919_length_2600_cov_3.273870"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLI11_08610"
     CDS             <1..981
                     /locus_tag="PLI11_08610"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HPZ52965.1"
                     /translation="KFAEYDQRKKAERDIRNLLKEYEAVVIPPEVYRTLPSVVRGIDS
                     ASLEGRMLSETESQLALNSLAIVHSIVSLKEDFKSFKEDPLDYLTKGVLIDDYIMTYF
                     GSFVKEMETRPQYLRSEIEYIIAGTSYESTNRLICESLIFAIRFVLNVLHIMCDADKV
                     DLANKIGNAIAGSITCGIGGPLYAVLIVAGWSICESMIDIKDLTEGRRVPFLKRKSEW
                     RFHIDGLPGSILDDELNGSSFFSMDYNDYLRLLLPFVGRETKLLRICDLIELNMTAFL
                     NRRYSINGMYASIKVSITVDVETLISYSLLPNLKKDDYFFEISEVYHGSY"
     gene            968..1948
                     /locus_tag="PLI11_08615"
     CDS             968..1948
                     /locus_tag="PLI11_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52966.1"
                     /translation="MVRTDKKGSVTVETAITLPLFISVLLIFSSFLICIAAVNSMYSA
                     LYKTASFFSDYGYIYHEKGIKKVEDSLLKDIGSLLDDNGTGLILKQANMRQLVEGADN
                     YVYGEIAKSIFLQYLNEENVIKSGLVKFDNVTFAGSSFFDHGDDVIFIVRCVMKLPLP
                     VPEKLVEGFELRGRLRFRCFIIGEMGSYNEAAPVETSVWSLNNFERGRIIRQVFGANL
                     PSNYPVIAKFDNGKATMIKSLDHTAKTYQDVNTFKMAIKSMILELSEFNGRDYGGVSI
                     KAGDIKRRELILVMPTNDMNPAQEFALMEISSYCLAKNIVLIVERYQEKK"
     gene            1999..>2600
                     /locus_tag="PLI11_08620"
     CDS             1999..>2600
                     /locus_tag="PLI11_08620"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPZ52967.1"
                     /translation="MWFDKACQAADYIKMKITEKYKDYKAPKVCIVLGSALGSFADRI
                     ESPLIIPYRDIEGFSVSTVSGHAGRLIYGFVKENPILCMQGRTHIYEGNSPQQVVLPL
                     RACTLLGAKNVVLTNAAGAINYDYNVGEFMVIEDHLSFFLDSPLFGKNEDDLGLRFPS
                     MDEAYDKELRKIAIECAKELGITVHSGIYAYMKGPQFETPA"
BASE COUNT          800 a          390 c          629 g          781 t
ORIGIN      
        1 aagtttgcag aatacgatca gagaaagaag gcagaaaggg atattcggaa tctgcttaag
       61 gagtatgagg cggtggtaat acccccagag gtgtacagaa cccttccttc agtagttagg
      121 ggaatagatt ccgctagtct ggaagggaga atgctgtctg aaaccgaatc ccagcttgca
      181 cttaactctc ttgcaattgt acatagtatt gtttcattga aggaggattt caagagtttc
      241 aaggaagacc cgcttgatta cttgacaaag ggtgtattga tagatgacta cataatgacg
      301 tatttcggca gttttgttaa ggagatggaa acaagacctc aatacttaag aagcgagata
      361 gagtacatta tagcggggac ttcatacgaa agtacaaaca ggcttatctg tgaatccttg
      421 atatttgcga taagatttgt tctcaatgta ttacacataa tgtgtgatgc tgacaaggtt
      481 gatctggcaa acaagatagg aaatgcaata gcaggaagca taacctgtgg gataggggga
      541 ccattgtatg cggttttaat cgttgcgggg tggtccatct gtgagtcgat gattgatata
      601 aaggatttga cagagggtag aagggttccc tttttaaaaa gaaaaagcga gtggaggttt
      661 catatagatg gacttcctgg tagtatactc gatgatgagt tgaatggctc ttcttttttt
      721 agtatggact acaacgatta tttaagactg ctcctgcctt ttgtaggcag agagacaaag
      781 cttttgagaa tatgtgactt gattgagctt aacatgactg catttcttaa cagaagatac
      841 agtataaacg gaatgtatgc atctattaaa gtaagtatta ctgttgatgt agaaactttg
      901 atttcatact ccttgttacc aaatctcaag aaagatgact atttctttga aatttcggag
      961 gtttatcatg gttcgtactg acaaaaaagg ctctgttact gttgaaacgg ctataacgct
     1021 gcctttgttt atctcggtgc tgctgatatt ttcatctttc cttatttgta ttgcggcagt
     1081 aaacagcatg tattcagcct tatataaaac ggcttccttc ttttccgatt atggttacat
     1141 atatcatgaa aagggaataa aaaaggttga agattcactt ttgaaagaca ttggcagtct
     1201 tttggacgat aatggaaccg gtcttatact gaagcaggcg aatatgagac aacttgtaga
     1261 aggtgcagac aactatgttt atggagaaat tgcaaaaagc atattcttgc agtatttgaa
     1321 tgaagaaaat gttattaaga gcggactggt aaagtttgat aatgtgactt ttgcaggaag
     1381 tagctttttt gatcatggcg atgatgtaat atttattgtg cgttgtgtta tgaagctgcc
     1441 tctaccggta cctgagaaac tggttgaagg atttgaacta agggggaggt taaggtttcg
     1501 ctgtttcata attggggaaa tgggctcata caatgaagct gcgccggtag aaacgtctgt
     1561 ctggagttta aacaactttg agaggggaag aataattagg caggtgtttg gtgcaaacct
     1621 accttcaaac tatcctgtga ttgcaaagtt cgataatgga aaggcaacaa tgataaaaag
     1681 tctcgaccat acagcgaaaa cgtaccagga tgtaaacaca tttaaaatgg caataaagag
     1741 catgatattg gagctttcgg agtttaatgg gagagattac ggtggtgtat ccattaaagc
     1801 aggggatata aagaggaggg aacttatatt ggtgatgcca acaaatgata tgaatccggc
     1861 gcaggagttt gctcttatgg agataagttc ttattgtctg gctaagaata ttgtacttat
     1921 tgtggagagg taccaagaga agaaatgact tgttacaatt tctatattct gttataatac
     1981 cggcaggagg gataaaaaat gtggtttgat aaggcatgcc aggctgcaga ttatatcaaa
     2041 atgaaaataa cggaaaaata caaggattac aaagctccta aagtttgtat tgtgctgggt
     2101 tctgcgctgg gcagttttgc ggatagaatt gagtctccac taattatacc gtatagagat
     2161 attgagggtt tttcagtttc cacggtaagc ggacatgcag gtagacttat ttatggtttt
     2221 gttaaggaga accccattct gtgcatgcag ggccgtaccc acatatatga gggaaattcg
     2281 cctcaacagg ttgttcttcc tttaagagca tgcactctgc tgggtgcaaa aaatgtagtt
     2341 ctgacaaatg ccgcaggtgc cattaactac gattacaatg ttggagaatt tatggttata
     2401 gaagatcatt tgagtttctt tcttgattct cccttgtttg gtaagaatga agatgatttg
     2461 ggtctcaggt ttccttccat ggatgaggct tatgataaag aattaagaaa aattgctatt
     2521 gaatgtgcaa aagaattagg aataactgta cacagcggta tatacgcata catgaagggg
     2581 cctcagtttg agactcctgc
//
LOCUS       DAONVY010000341         2598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26942_length_2598_cov_3.353913, whole genome shotgun sequence.
ACCESSION   DAONVY010000341 DAONVY010000000
VERSION     DAONVY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26942_length_2598_cov_3.353913"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLI11_08625"
     CDS             <1..303
                     /locus_tag="PLI11_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HPZ52968.1"
                     /translation="KHTKLCHMNYNFNNVIKNEKEVFILEWENALLGNASADVANTYL
                     ILRMENEELAELYLSKFCEFTCTPKAYVQKWLPIVAASMHGKKDKFHDLINVVEYY"
     gene            558..>2598
                     /gene="adhE"
                     /locus_tag="PLI11_08630"
                     /gene_synonym="adhC"
     CDS             558..>2598
                     /gene="adhE"
                     /locus_tag="PLI11_08630"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HPZ52969.1"
                     /translation="MIINSIEALTEKLAKVKEAQEKFAGFTQEQVDRIFYEAAMAANK
                     KRIPLAKMAVEETGMGVVEDKVIKNHYASEYIYNAYKHVKTCGVIQEDESFGIVQIAE
                     PVGVLAAVIPTTNPTSTAIFKALIALKTRNGIIISPHPRAKKCTIEAAKIVYEAAVKA
                     GAPEDIIAWIDVPSLEMTNYIMANCDLILATGGPGMVKAAYSSGKPAIGVGPGNVPAI
                     IDDSADLVLAVNSIIHSKTFDNGMICASEQSVIVLDSIYDKVKEEFAKRGCYILGDNE
                     IDKVRKTIIINGSLNAKIVGQSAHKIGELSGIEVPADAKILIGEVTDVSLNEEFAYEK
                     LSPVLAMYRAENFEDALDKASVLVKDGGLGHTASIYLNEAAEKEKLENFARRMKTCRI
                     LVNTPSSHGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVGVKHLVNIKTVAKRRENML
                     WFRTPEKVYFKKGCLPVALMELNEVLNKKRVFIVTDSFLYKNGYTKVVTDKLDEMGIV
                     HKTFYDVQPDPTLENALKGAAAMNSFEPDCIIAIGGGSAMDAAKIMWVLYEHPDVDFH
                     DMAMVFMDIRKRVYTFPKMGEKAYFIAIPTTAGTGSEVTPFAVITDEKTGIKYPLADY
                     ELLPDMAIIDVDLMMSMPKGLTAASGIDALTHSLEAYASMMATDYTNGLALTSIKL"
BASE COUNT          846 a          479 c          584 g          689 t
ORIGIN      
        1 aaacacacaa aactttgtca catgaactac aattttaaca atgtaataaa gaatgaaaag
       61 gaagtattta ttctggagtg ggagaatgcg cttctgggta atgcaagtgc ggatgtggca
      121 aacacttatt taattcttag aatggagaat gaagaactgg cggaacttta cttaagcaaa
      181 ttctgtgaat tcacctgcac tccaaaagcc tatgttcaga aatggctgcc aattgtagca
      241 gcaagcatgc atggcaagaa ggacaagttc catgacctta ttaatgttgt tgaatattat
      301 taaatggaaa tattgagaac ccgccaattt tggcgggttt tttgctatag tggaggaagt
      361 gcaagttttg ttaatcaaat taaacagttt tatataaaaa cgacattgtt aaaagattaa
      421 caaataggac atacaagaat gataaattct taacaatctt gtattgacac ctgtataggc
      481 agaacatata atattgttta ataaataaca aagtttggat tgttatttat ttacaagtga
      541 caaggaggaa aaagattatg attattaaca gcatagaagc tttaactgaa aagctggcga
      601 aagtaaaaga agcacaagaa aagtttgctg ggtttacgca agagcaggta gacagaattt
      661 tctatgaagc ggcaatggct gcaaacaaga aaagaatacc tcttgcaaag atggctgttg
      721 aagaaaccgg aatgggcgtt gtggaagaca aagttattaa aaaccactat gcctccgaat
      781 atatatacaa tgcatacaaa catgtaaaga cttgtggagt aatccaagag gatgaatctt
      841 ttggtattgt gcaaattgca gaaccggtag gagtacttgc ggcagtaata ccaaccacca
      901 atcctacatc aactgcaata ttcaaagctt taattgcatt gaagactaga aatggcataa
      961 ttatcagtcc tcatccaaga gcgaagaaat gcacaatcga ggctgctaaa atcgtatacg
     1021 aagctgcagt gaaggcaggt gctcctgaag acattattgc atggatagac gttccatcac
     1081 tggaaatgac aaactacata atggccaact gcgaccttat ccttgcaaca ggtggtcccg
     1141 gcatggtcaa ggcggcatat tcctcaggta aacctgctat aggtgttggc ccaggaaacg
     1201 ttcctgcaat aattgacgat tctgcagatt tggttcttgc agtcaactca attatacatt
     1261 ccaagacttt tgacaatgga atgatttgcg catccgagca gtcggttatt gttcttgaca
     1321 gtatttatga caaagtaaaa gaggagtttg caaaaagagg atgctacatc cttggcgata
     1381 atgagattga caaggttaga aagactataa ttataaatgg ttccttgaat gccaaaatag
     1441 ttggccagtc cgctcacaag attggagaac tttcaggaat cgaagtacct gctgatgcaa
     1501 agatactgat aggtgaagtt accgatgtat ccttgaatga agaatttgct tacgagaagc
     1561 tgtcaccggt acttgcaatg tacagagctg aaaacttcga ggatgctctt gacaaggcca
     1621 gtgtgctggt taaagacgga ggacttggcc acaccgcttc aatatacctg aatgaagctg
     1681 ctgaaaaaga aaagcttgag aattttgcac gcagaatgaa aacctgcaga atacttgtca
     1741 acactccatc ctcccatggt ggaataggtg acctttacaa cttcaagctt gctccatccc
     1801 ttactttggg ctgtggttcc tggggcggaa actccgtatc cgagaacgta ggagtcaagc
     1861 atcttgttaa tattaagact gttgcaaaaa ggagagagaa tatgctttgg ttcaggaccc
     1921 ctgaaaaggt ttatttcaag aaaggttgtc tgcctgttgc ccttatggaa ctcaatgaag
     1981 ttttgaacaa gaagagagta ttcatagtta cagatagctt tctgtacaag aatggttata
     2041 caaaggtagt aaccgacaag cttgacgaga tgggaatagt gcataaaact ttctatgatg
     2101 tgcagcctga tccaactctc gagaatgctt tgaaaggcgc agctgccatg aactccttcg
     2161 aacccgactg cattattgca ataggtggag gatccgcaat ggatgctgca aaaatcatgt
     2221 gggttctcta tgagcatcca gatgttgact tccatgacat ggcaatggtg ttcatggata
     2281 ttagaaagag agtatacaca ttcccgaaga tgggcgagaa ggcatacttc atcgcaatac
     2341 caacgacggc aggcactggt tccgaggtta ctcctttcgc cgttataacc gacgaaaaga
     2401 ccggtatcaa gtatcctttg gcggattatg agctgcttcc agacatggct attatcgatg
     2461 tggatttgat gatgtccatg ccaaagggat tgactgccgc ctccggtata gatgctttga
     2521 ctcacagcct tgaagcttat gcatccatga tggcaaccga ctatacgaac ggtttggctc
     2581 tcacatccat aaagctca
//
LOCUS       DAONVY010000342         2596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_26963_length_2596_cov_3.601732, whole genome shotgun sequence.
ACCESSION   DAONVY010000342 DAONVY010000000
VERSION     DAONVY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26963_length_2596_cov_3.601732"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLI11_08635"
     CDS             complement(<1..717)
                     /locus_tag="PLI11_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ52970.1"
                     /translation="MRKSADTNLLKLLYVPISVAIITLIVIGISLGTMPVVKPDNTPQ
                     PTEEPVDIKVVITGSHEPGYYPEGFDFSLECSDENAKIYYTITSPSRRSTSPDEKVAP
                     TGEVYLYAGGKLPHPSKVDEEGVRQSILYTKPIAIPSQSESAIESSRMTVIIAAAFDG
                     DVRVSDYYYYTFTIDGKGQEPNYQDKFGSYLVSIIIDEEDLYDYERGIMIQGKAFQDE
                     RTRNPRREIDGWFPRNYNQRG"
     gene            complement(844..2217)
                     /locus_tag="PLI11_08640"
     CDS             complement(844..2217)
                     /locus_tag="PLI11_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPZ52971.1"
                     /translation="MSKKVPYKIYLTEEQMPKQWYNMLADMKVKPAPYLNPATLEPLG
                     VTDLYPIFCEELAKQELNTQDRYIDIPEGILNFYKIYRPSPLIRAYNLEKALGTPAKI
                     YFKFEGSNTSGSHKLNSAAAQVYYAKEQGLKCLTTETGAGQWGTALAMACAHFGLDLK
                     VYMVKISSQQKPYRKTVMETFGAEVIPSPSETTKVGREILKNDPNTHGSLGCAISEAI
                     EASMSRDDCRYVLGSVMNQVILHQSIIGLEAKKAMELVDEYPDIVIGCAGGGSNLGGL
                     IGAFMQDKFAGRKAPYFIAVEPASCPSFTRGKFAYDFCDTGRVTPLAKMYTLGSDFKP
                     SPNHAGGLRYHGMSPMLSQLYYDGYIDEARAVEQSAVFEAACLFAKHETILPAPESSH
                     AVRVAIDEALKCKETGEAKKILFGLTGTGTFDMTAYSSYISGEMVDYIPTDEELEASF
                     SRLPKVE"
     misc_binding    complement(2274..2524)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          659 a          639 c          488 g          810 t
ORIGIN      
        1 tcctctttgg ttgtagtttc ttgggaacca gccatcaatt tcacgtctgg ggtttctggt
       61 gcgttcatct tggaaagcct ttccctgaat cattatccca cgctcgtaat catacaggtc
      121 ttcttcatca atgataattg acaccagata gcttccaaat ttgtcctgat aattcggttc
      181 ctggcctttt ccgtcaattg taaacgtgta gtagtaataa tcgctgactc ttacatcccc
      241 gtcaaaggca gcagcaataa tcacagtcat tcttgatgat tcaattgccg actccgactg
      301 gctgggtatt gcaatgggct tggtatatag aatgctctgt cttacccctt cctcatcaac
      361 cttgctcgga tgaggaagct ttcctccagc atacaaatac acttctcctg taggagcaac
      421 cttctcatca ggagaggtgg accttcttga aggacttgtt atcgtataat atatcttcgc
      481 gttttcatca ctgcactcaa gagaaaaatc aaaaccttcc ggatagtatc caggttcgtg
      541 ggagcctgtt ataacaacct ttatatctac cggttcttct gttggctgtg gtgtattatc
      601 cggctttaca accggcatcg taccaagact tatacctatt actatcaatg taattatcgc
      661 aactgatatt ggtacataca acaatttaag caaattagtg tccgcactct ttctcaaact
      721 tgattacacc tctacatatc cttgttaata ttaagagttt agcaccaaaa gcggactaaa
      781 tcaatataat ctttaaaagc aagttgcggc aaccaaaatc tattgccgca acctgtatgt
      841 tccctattct acttttggaa gccttgaaaa acttgcctca agttcttcat cggttggaat
      901 gtaatccacc atttcgcctg aaatatagct tgaatatgcc gtcatgtcaa aggttcctgt
      961 tcctgtgagg ccaaacagta ttttttttgc ttctcctgtt tccttgcact taagagcttc
     1021 atcgattgca acccttacag catgacttga ttcaggagca ggaagtatgg tttcatgttt
     1081 tgcaaacagg caggcagctt caaatactgc actctgctcg accgccctgg cctcatcaat
     1141 atagccgtca tagtaaagct ggctgagcat tggcgacatg ccgtggtatc taagtcctcc
     1201 tgcgtgattt ggtgatggtt tgaagtcgct tcccaatgtg tacatctttg caagaggagt
     1261 tactcttccg gtgtcgcaga agtcatatgc aaacttgcct cttgtaaatg acggacatga
     1321 tgcaggttct actgcgataa agtatggagc ctttctgcct gcaaacttat cctgcatgaa
     1381 agcgccaata agaccaccaa ggtttgatcc gcctccggca caaccaatta ctatgtctgg
     1441 atactcatcc acaagctcca ttgccttctt tgcctcaagc ccaataatcg actgatgcag
     1501 aataacctgg ttcataaccg aacccaacac atatctgcaa tcgtccctgg acatggatgc
     1561 ctcgatggct tcagatatgg cgcaaccaag gctgccgtgg gtgttgggat cgttcttcaa
     1621 aatctccctg cccacctttg ttgtttcact cggacttggt ataacctcag ctccaaaggt
     1681 ttccataaca gtctttctgt acggcttctg ctggctgctt atctttacca tgtatacctt
     1741 taggtcaaga ccaaagtgcg cacacgccat ggcaagagca gtaccccatt gtcctgcacc
     1801 ggtttcggtg gtcagacact taagtccctg ttccttggcg taatacacct gtgcagctgc
     1861 agagttcagc ttgtggctgc cggatgtatt gctgccttca aacttgaaat atatctttgc
     1921 aggtgtgcca agagccttct ccagattgta tgccctaatc aatggagatg gcctgtagat
     1981 cttgtaaaag ttcaaaatcc cctctggtat gtcaatatat ctgtcttgcg tgttcaactc
     2041 ctgctttgcc agttcctcgc aaaatatagg gtacagatcc gtaactccca atggttccaa
     2101 tgtggcagga ttcaaatatg gagctggttt aactttcatg tccgccagca tgttgtacca
     2161 ctgtttcggc atctgttcct ctgtaagata aattttgtac ggtacttttt ttgacatttt
     2221 caacctctcc ttaattctat attttcccaa taaaaaaact cttgcccttg aaatataagg
     2281 acaagagtac aattctcctg cggtaccacc ttaattggtc tttcgacccg ctcattccat
     2341 acaccatcat gtatgctccc ttgataatgg gtggagatcc catcagctac tactccagat
     2401 ttaatctgtt tcggtctgcc ctcatgagtc cattcagttg gtacttaaat acagcgctct
     2461 caccatatac gccgctctct ttgattcaag aagccaactt actcttctca atcacaggtt
     2521 taaattttaa attttgagta ttatacagca aagaataata attgtcaaca cccatttatt
     2581 aattaacagt tttatg
//
LOCUS       DAONVY010000343         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27235_length_2574_cov_4.373958, whole genome shotgun sequence.
ACCESSION   DAONVY010000343 DAONVY010000000
VERSION     DAONVY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27235_length_2574_cov_4.373958"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /gene="cutA"
                     /locus_tag="PLI11_08645"
     CDS             <1..316
                     /gene="cutA"
                     /locus_tag="PLI11_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPZ52972.1"
                     /translation="SEYGIVLTTFANEQQVKQVIDEILECKLAACVQEIKIKSHYVWK
                     GELCREDEVLVLLKTRKELYPELEKKLKEIHPYETPEILLVDVELGSLAYLKWLDEQT
                     RK"
     gene            426..1625
                     /locus_tag="PLI11_08650"
     CDS             426..1625
                     /locus_tag="PLI11_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPZ52973.1"
                     /translation="MGIYQKCASFTKVDEVKEKGIYPYFHHLESRQDVEVIMEGKRRI
                     MLGSNNYLGLTTHPEVMKAAIKAIEKYGTGCSGSRFLNGTLDMHLQLEEELAEFVGKE
                     AACTFPSGFQTNLGIISAIVGRHDYIISDRENHASIYDGCRLSFGKTVRYKHDDMEDL
                     EQCLAKLPLDAGKIIITDGVFSMSGDICNLPKIVELARKYNAVTMVDDAHGFGVLGNG
                     GRGTAEHFGLTEETDIIMSTFSKSLASLGGFVASDKYVIEYIKHNSRPFIFSASIPPA
                     SCAAALAALRVIKKNPELVSRLSDLALYMRNGLKERGIPVRESVYTTPIIPFYTYDFE
                     TTLIRAKEIYDAGVYLNPVLPPAAPDGNCLLRISLMATHTYELLDEAMDIIHSVINKS
                     VKNGCDC"
     gene            1626..2417
                     /locus_tag="PLI11_08655"
     CDS             1626..2417
                     /locus_tag="PLI11_08655"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPZ52974.1"
                     /translation="MKKTVVITGASSGIGYYAAKFFLEGGYNVYGLSRKAGDLAGVNY
                     ISTDLTNEESVNAAFDKIVAETGAVDILVNNAGSGISGAVEFTEMSEAKYQFDVNFFG
                     MVRCIKCALGIMRKKGGKIINVSSVAAAFPLPFQAFYSASKSAVNALTMSLRNEVRKF
                     GNIQVCAIMPGDVKTDFTSSRRKNETGDDVYGGIISNTISGMERDEENGMSPEYMGWL
                     IYKVATKRKLKPFYIGGHRYKVFVFLERLLPKSFVSYVLAKMYIK"
BASE COUNT          791 a          435 c          614 g          734 t
ORIGIN      
        1 gagcgaatat ggaattgttt tgactacatt tgctaatgag cagcaggtaa aacaagtaat
       61 agacgagata ctggaatgta agttggcggc atgtgtacag gaaataaaga taaagagtca
      121 ttatgtatgg aaaggtgaac tctgtcgtga agatgaagta ttggttttgt taaagacaag
      181 aaaggagtta tatcctgaat tagaaaagaa acttaaggaa atacacccct atgaaacacc
      241 agaaattctt cttgtagatg tggagttggg aagtcttgcg tatttgaaat ggctagatga
      301 gcaaactcgc aaatagtatc agaaggttgt gaacattata ttaaaataat tgttaacatc
      361 tcaaacctcg tatggtataa tagcacaagt attgtagatt ttactatctt tggaggtttg
      421 tgtacatggg aatttatcag aaatgcgcaa gtttcactaa agttgacgaa gtaaaagaaa
      481 aaggaattta tccatatttc caccacttgg aatcccggca ggatgtggag gtaataatgg
      541 aaggcaagcg cagaattatg ctgggatcaa acaactatct tggactcaca actcaccctg
      601 aagttatgaa agcggctatt aaagctattg aaaaatacgg tacaggttgc tccggatctc
      661 gttttttaaa tggtaccttg gacatgcatt tacagcttga agaagagttg gctgaattcg
      721 ttggcaagga agctgcatgc acatttccgt cgggcttcca gacaaatctc ggcattatta
      781 gtgccattgt agggcgacac gactatatta ttagtgacag ggagaatcat gcaagcatat
      841 acgacgggtg caggttgagt tttggcaaaa ccgtccgcta caaacatgac gatatggagg
      901 atttggagca gtgccttgca aagcttcctc tggatgccgg caagattatt atcacagatg
      961 gtgtgttcag catgagtgga gatatttgta atcttcccaa aattgttgag ttggccagga
     1021 agtataatgc agtcactatg gtggatgacg cccatggatt tggtgttctt ggaaatggtg
     1081 gcaggggaac ggcggaacac tttgggctga cagaggagac ggatataata atgagtacat
     1141 ttagcaaatc tctggctagt ctcggtggct ttgtggcatc ggacaagtat gttattgagt
     1201 atattaaaca caactcaaga ccttttatct tctcagcctc aattccaccc gcatcatgtg
     1261 cggcggcttt ggcagcatta cgtgttataa agaagaatcc cgagctggtt tctagattgt
     1321 cggatttggc gctatacatg agaaatgggc ttaaggaaag gggaattccg gtcagagaat
     1381 ccgtatatac aacacctatt atcccattct atacatatga ttttgagaca acactcataa
     1441 gagcaaagga aatttacgat gcaggtgtct atttaaatcc tgttcttccg ccggcagctc
     1501 ctgatggcaa ctgcctgctt agaatcagct tgatggcaac tcatacatat gaactgctgg
     1561 acgaagcaat ggatattata cactcggtta ttaacaagag tgttaagaat ggttgtgatt
     1621 gttaaatgaa gaagacggtt gtgattactg gagcctccag tggcattggc tattatgcag
     1681 caaagttttt tctggagggt gggtataatg tatacggtct tagcagaaag gctggagacc
     1741 ttgcaggtgt caattacata agcactgacc ttacgaacga agaatccgtc aatgcggctt
     1801 ttgataaaat tgttgctgaa actggtgcag ttgacatcct tgttaacaat gccggttcag
     1861 gtatatccgg tgctgtcgag tttactgaaa tgtcggaagc taagtaccag tttgacgtaa
     1921 acttttttgg catggtgcgc tgcataaaat gtgcactggg cataatgcgc aagaagggag
     1981 gaaaaattat aaatgtcagc tctgtggcag ctgcttttcc gctcccattc caagcctttt
     2041 actcagcttc caaatcagca gttaatgctc taaccatgtc gctaagaaat gaagtaagga
     2101 aatttgggaa tattcaggtt tgtgcaataa tgcccggaga tgttaaaacc gattttactt
     2161 catcaagaag aaaaaatgaa acaggagacg atgtgtatgg cggaatcata tccaatacca
     2221 tttccggcat ggagcgtgat gaggaaaatg gcatgtcgcc tgaatatatg gggtggttga
     2281 tttataaagt agctacaaaa cggaaactaa aaccttttta tatcggagga cacaggtata
     2341 aggtatttgt atttcttgag agattgctcc ctaaaagctt tgtatcctat gtattggcga
     2401 aaatgtatat aaagtaaaat aacagaagaa gaccgattca actaaaaatt tcggtcttct
     2461 tttatatatt gcttttattc ttgtgccgcc tcagtagttt ctaaatttac tttggacgga
     2521 gctggtgacc tcttgcctaa gaatagaaac ctggttagtc ggaaacgatt taaa
//
LOCUS       DAONVY010000344         2569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27315_length_2569_cov_3.846062, whole genome shotgun sequence.
ACCESSION   DAONVY010000344 DAONVY010000000
VERSION     DAONVY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27315_length_2569_cov_3.846062"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLI11_08660"
     CDS             <1..434
                     /locus_tag="PLI11_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52975.1"
                     /translation="IPGAFLAYQEVIDFLVSVNTKGYIESIEENLDGSITYIITKENY
                     IYILSFLKVNIINLFEELKTSSQYPSIKDVTYNEDFTNITFYVAKKDYENSKDEVASF
                     VIAAKSMVYSFLSNNNIEIPPVIVKIKDHETKEILKSISWP"
     gene            complement(431..>2569)
                     /locus_tag="PLI11_08665"
     CDS             complement(431..>2569)
                     /locus_tag="PLI11_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPZ52976.1"
                     /translation="SKDSIGMDKLKGKYELLIRESCDMFALVNDKGQIKYASESLSEV
                     IGYDGSELLGKSIFDIFNTENSIEIKYMIEKVSEGSEKKAEGTFFYTNKNGDNIYLEL
                     VIRNVNQKSRNGLIVSFRDITKRVEMEKMVHHISTHDELTGLPNRIHLKETLNKLCNE
                     AKKENNKFATMMLRVDRFNRMLDAIGHYVGDKLLVEVSHRLKYVFGEHHYICRYSEDR
                     YAIIVTDMESISDYEAMARKAINVFSNSFTVDKYDIEVTINMGICLFDENNSDAESII
                     KHAEIALFWAKNEGKNKYKFYSSDIDVQTYKQFELHADMLKAIDNDQFRVYYQPIMDL
                     KTNRVIAAEALIRWDHPTWGMVSPMEFIPIAEETGYIMNIGRWVFKKVCEDIKKWISY
                     GLRPVKVGVNISSVQFFESRFVENIIKTIEDHSLDPNLLIIEITESILLVNTEKVIND
                     LKRLREAGIQIALDDFGTGYSSLAYLSSFNIDILKMDSSFIKNLSADYASTTITRHII
                     NMAHELSLKVVAEGIETMPQLVFLKDLKCKTGQGYIYSKPLPEDMFRKILKKRVVRPE
                     AKEPTIVSDKNKRKYFRVEFKNLLIADMTILELKGKELNVGNTKVLVKNISAGGLCFV
                     TNLKMPVSRDLVMNFSAYLAGKILDIRGYAVWMKEHDKNLYEYGVEFAIDERDREHLM
                     KDLWYIQMQIKTNDVFTDGDFAVSANEYFN"
BASE COUNT          719 a          581 c          435 g          834 t
ORIGIN      
        1 ccatcccggg agcttttctt gcatatcagg aggtcattga ttttctcgta agtgtaaata
       61 caaaaggata tattgaaagc attgaagaaa acttggatgg ctccataacc tacatcatta
      121 ccaaagaaaa ctatatttat attctaagct ttctcaaagt aaatattata aatctatttg
      181 aagaactcaa gacaagcagt cagtatccgt ccataaagga tgtgacatac aatgaagatt
      241 ttacgaatat aactttttat gtggcaaaaa aagactatga aaacagtaag gatgaggtag
      301 catcctttgt catagcagca aagagcatgg tttactcctt tctttcaaac aacaacattg
      361 aaataccgcc tgtaattgtc aagatcaagg accatgagac taaagaaata ttaaaatcca
      421 tctcctggcc ctaattaaaa tattcgtttg cactaactgc gaaatcaccg tctgtgaaaa
      481 cgtcgttagt cttaatctgc atctggatgt accaaagatc cttcataagg tgttccctgt
      541 ccctctcatc tatggcaaat tcaacaccgt attcatacag gttcttgtca tgttctttca
      601 tccagacggc gtaccctctg atatccaata tttttcctgc caggtaggca gaaaaattca
      661 taaccaaatc cctgctgacg ggcattttca gatttgtgac aaaacacaat cctcctgcac
      721 tgatattctt caccaatacc ttcgtattgc ccacattcag ctcctttccc ttaagctcaa
      781 gaattgtcat gtctgcaatc aacaagtttt taaactccac cctgaaatac tttcttttgt
      841 tcttgtcaga cacaattgtt ggctcttttg cttccggcct tactactctc ttcttcaata
      901 tcttcctgaa catgtcttca ggaagaggct tgctgtagat gtaaccctgg ccggttttac
      961 acttcaagtc cttgagaaaa acaagctggg gcattgtttc aataccctcc gccaccactt
     1021 tcagactcag ttcatgtgcc atgttgatta tgtggcgggt tatagttgtg cttgcatagt
     1081 cggcgctcaa gtttttaata aaggagctgt ccatttttaa aatatctatg ttaaaagaac
     1141 tcaaatacgc cagcgacgaa tatcccgtac cgaagtcgtc caaggctatc tgaatgcccg
     1201 cttcgcgcaa tcttttcaag tcattaatga ccttctcggt attaacaagc aaaatgcttt
     1261 cagtaatctc tataattaag agattagggt cgagagaatg gtcttctatt gttttaataa
     1321 tattttcgac aaagcgggat tcaaagaact ggacacttga tatgtttaca ccaaccttta
     1381 caggtctcaa gccatacgaa atccacttct ttatatcctc gcataccttt ttaaacaccc
     1441 atcttccaat attcatgata taaccggttt cttcggcaat gggtatgaat tccatcggcg
     1501 ataccatacc ccaggttgga tgatcccatc tgataagagc ctctgcggca attaccctgt
     1561 tggtcttcaa atccattatg ggctggtagt ataccctgaa ctggtcattg tcaatggctt
     1621 tgagcatgtc tgcgtgtaat tcaaactgct tgtatgtttg cacgtcaata tccgaggaat
     1681 agaacttgta tttgttcttc ccctcatttt ttgcccaaaa cagtgcaatt tcagcatgct
     1741 tgattatact ctccgcatcg ctgttattct cgtcaaaaag gcagattccc atgtttatag
     1801 taacttcaat atcatattta tccacagtaa acgagttgct gaatacattt attgccttcc
     1861 tagccatggc ctcataatca cttattgatt ccatgtctgt gacaatgatg gcatatctgt
     1921 cttcggaata gcggcagata tagtggtgtt ctccaaacac atactttagt ctatgggaaa
     1981 cctccactaa aagtttgtcc cccacataat gacctatcgc atccagcatt ctgttaaatc
     2041 tgtccaccct gagcatcatg gtggcaaatt tattattttc ctttttagct tcattgcaaa
     2101 gtttgttcag ggtttccttt aggtgaatcc ggtttggaag accggtaagt tcgtcatgtg
     2161 tggatatgtg atgaaccatt ttctccatct ccaccctttt ggttatgtct ctgaaagaca
     2221 caataagacc gttccgggac ttctggttca cattgcgtat gacaagctcc agatatatgt
     2281 tgtccccgtt tttatttgta taaaaaaagg tgccttcagc ctttttctca gaaccttccg
     2341 atactttttc aatcatgtac tttatttcaa tggaattttc cgtgttgaaa atatcgaata
     2401 tgctctttcc aagcaactcc gaaccatcat aaccgattac ctctgacaat gattcgcttg
     2461 catattttat ctgaccttta tcgttaacca gagcaaacat gtcgcaggat tcccttatca
     2521 aaagctcgta ttttcctttg agcttgtcca taccaatgga atctttgct
//
LOCUS       DAONVY010000345         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27439_length_2561_cov_3.474860, whole genome shotgun sequence.
ACCESSION   DAONVY010000345 DAONVY010000000
VERSION     DAONVY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27439_length_2561_cov_3.474860"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLI11_08670"
     CDS             complement(<1..284)
                     /locus_tag="PLI11_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52977.1"
                     /translation="MIGQVLNSIHDGKITDYISSFILDRLREKYDVEFDVLAIGNYDM
                     ASGRVVAVCCARDDSKICFKVIADRKNIQDSYIRAYAAREVEKALLFNLY"
     gene            complement(378..1574)
                     /locus_tag="PLI11_08675"
     CDS             complement(378..1574)
                     /locus_tag="PLI11_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52978.1"
                     /translation="MVNYYELLNVSRDATKEEIELALARCMLSPNKNNDISLAFDILT
                     KETIRKLYNYSLDVPLEVGEIIQSSLKKYIEKNAHIAIDILNEGLKHYPDQREIMLVL
                     SRMYCFTGQAQESINVLVVLYENYPDDNDVVFSLGYAYNCLRNYSTSYLYLRTLYKTL
                     NRDVEYLNLYGEACVHLGKYERAYCIAAHNIKHNYGYDELIYSYVYLIIAQYYINKNV
                     LENSVGYFIKYMHEEVSDVSLAGDCLDKLLHFVLDNVMQDEDVSLLIDVLVKIYRIVG
                     ANNARAEVVLFACDLSLECLRMVESGINEKISSLCKNYICLLLSDVFHDLKRETYEAK
                     IAINKLEIVELYDFTYEEIGQKLPDEYPLMTELVSDFINDFRQLDLEELVDKYNKLYE
                     NMINGE"
     gene            complement(1603..>2561)
                     /locus_tag="PLI11_08680"
     CDS             complement(1603..>2561)
                     /locus_tag="PLI11_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPZ52979.1"
                     /translation="LNSDIKEIRVFSRDEKKQNDMRHKYSALYPEIAHKLKFYIGDVR
                     DLSSIRYAIYGVDYVFHAAALKQVPSCEFFPLEAVKTNVFGTDNVLTAAIDAGVSKVI
                     CLSTDKAAYPINAMGMSKAMMEKVVVAKSRTVSPEKTTICCTRYGNVMCSRGSVIPLF
                     IDQIKSNSPLTVTEPNMTRFIMSLDEAVELVLYAFQHAKSGDILVQKAPACKIGVLAQ
                     AVKEIFNSDAEIRIIGIRHGEKMYETLLTSEECANSIDLGNYYRVPVDKRDLNYDKYF
                     TKGDSDRSIATEFNSNNTVQLDVEEVKEKLLRLKYVQDELRN"
BASE COUNT          743 a          588 c          411 g          819 t
ORIGIN      
        1 tcatacagat taaaaagaag tgccttttcc acttcccttg cagcatatgc tcggatataa
       61 ctgtcctgga tgttcttcct gtctgcaatc actttaaaac atatttttga atcgtccctt
      121 gcacagcaaa cggcaaccac cctgccgctt gccatgtcgt aattgccgat tgccagtaca
      181 tcaaattcca catcatattt ctccctcaac ctgtcaagga tgaaggaaga aatataatca
      241 gttattttcc catcatgaat gctgtttaac acttgcccaa tcatatacca tacccaggca
      301 cttctttaat ataggtttat tcaaaagata aataaaaatg actgataaaa agaaaaaaag
      361 caaggaattt tccctgctca ttctccattt atcatgtttt catacaactt attgtatttg
      421 tccacaagct cttccaagtc cagttgtctg aaatcgttta taaaatctga taccagttca
      481 gtcattaatg gatattcatc cggcagcttt tggccaatct cctcatatgt aaaatcgtac
      541 aactcaacta tctcaagctt gtttattgca attttggcct cgtatgtttc tctcttcaaa
      601 tcatgaaata catcgcttaa taaaaggcat atataattct tgcacagact cgaaatcttc
      661 tcattgatac cactctctac cattcgaaga cattccagag aaaggtcgca tgcaaataga
      721 accacttccg cccgggcatt gttggcacca acaattctat atatcttcac taaaacatct
      781 atcagcaaag acacatcctc gtcctgcatt acattgtcca gaacaaagtg caaaagcttg
      841 tcaaggcagt ccccagccag agaaacatcg gatacttctt catgcatgta tttgataaaa
      901 taccccacac tattttcaag cacattttta ttaatatagt attgggctat tatgagataa
      961 acgtatgagt atataagctc atcatatcca taattatgct ttatgttatg tgcagcaata
     1021 caatatgctc tttcgtattt ccccagatga acacatgctt caccatacag gttaagatac
     1081 tctacgtccc tgttcaaggt tttgtagagg gttctaaggt ataaatagga ggtactgtag
     1141 ttcctcagac agttatacgc ataccccaaa gaaaaaacaa catcgttatc atctggataa
     1201 ttctcataaa gcacaaccaa tacattaatg ctctcttggg cctgtccagt aaaacaatac
     1261 attcgggaca atacaagcat tatctctctt tgatccgggt agtgtttcaa accctcattc
     1321 agaatatcaa ttgcgatatg tgcattcttt tctatatact ttttcaagga gctctggata
     1381 atttcaccaa cttccaaagg cacatccaaa gagtaattat acagctttct gatagtttct
     1441 tttgtcaata tatcaaacgc aagtgagata tcattgtttt tatttggtga cagcatacaa
     1501 cgggccaagg caagctctat ttcctctttt gttgcatctc ttgatacatt aagcagttcg
     1561 tagtagttta ccataggact ccttttcaat ttatttttta gtctagtttc tcaattcatc
     1621 ctgtacatat ttaagtctta ataatttctc ctttacttct tcaacgtcaa gctgaaccgt
     1681 attgtttgaa ttaaactccg tagcgatgct tctgtcgcta tctccttttg taaagtactt
     1741 gtcatagttg aggtccctct tgtccacagg cacccggtag tagttgccaa ggtcaatgct
     1801 gtttgcacat tcctcgctgg taagaagcgt ctcatacatc ttctcgccat gtctgatacc
     1861 gataatcctt atttcagcat cagaattaaa tatctccttc accgcctggg caagtactcc
     1921 aatcttacat gcaggagcct tttgaacaag aatatcaccg gattttgcgt gctggaacgc
     1981 ataaagcaca agttccaccg cctcatcaag actcattata aatcttgtca tgttgggttc
     2041 cgtcacagtc aatggactgt ttgatttaat ttggtctatg aacagaggaa ttacacttcc
     2101 tcttgaacac ataacatttc cgtatcttgt gcagcatatt gtggtttttt caggactcac
     2161 ggtgcgggac tttgccacca ccactttttc catcatggcc ttgctcatgc ccatggcatt
     2221 gataggatac gcagccttgt cagtagacag gcatattact ttagaaactc ctgcatctat
     2281 ggcagctgtt agtacattat cagtgccaaa cacattggtt tttaccgctt ccagcgggaa
     2341 aaactcacaa gaaggcacct gcttcaaggc agcggcatga aaaacgtaat caactccata
     2401 aatagcatac ctgatggaag acaggtccct tacgtcccct atatagaatt tgagtttgtg
     2461 ggcaatttcg ggatacaagg ctgaatactt atgcctcata tcgttttgtt ttttctcatc
     2521 ccttgaaaat acccttattt ccttgatatc gctattaaga a
//
LOCUS       DAONVY010000346         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27442_length_2561_cov_3.044693, whole genome shotgun sequence.
ACCESSION   DAONVY010000346 DAONVY010000000
VERSION     DAONVY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27442_length_2561_cov_3.044693"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="PLI11_08685"
     CDS             <1..1611
                     /locus_tag="PLI11_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52980.1"
                     /translation="KQAEMLADAGIDFVAFDCTNGSFTWEPAYLAVCEVWSEAREQGV
                     KTPQIIFMLNFGPLADTVVSLKSIYNRLYKKEAYKDLWFYWEGKPLIMAHPECLNQNN
                     ALEKEIYEFFTWRRNVASYFEDDKDDRYWGWLHVYPQAVYKNEDGTVEQVTVGIAQNA
                     DYVEWRLCAMNGPNNMGRSYTNGEYSYSYTYRNKTIKVDKNIENSMFYGLNFQQQFDF
                     AIKQDPEIIFVTGWNEWIMGRFDEWMGIENAFPDQFDDENSRDIEPSKGALKDYYYYQ
                     LIANVRRFKGASDIDYAEKGVSIDIFGDLSQWDDPAILSYDHYTKNTYERNSRGWGRL
                     KYENYTMRNDIVNTKVTYDDDNIYFYVRTVDDLTPHTDPAWMRLFLDTQRATETSVDW
                     EEFEYVINRRTPSDSVAYLERSKGGWDWEQVGEVRYSVNGNVLQIEVPREMLGFKNKP
                     INFNFKWSDNMQEDGDIMDFYLNGDVAPGGRLCFAFRDKAAYLEEKGDEKEGFSISTT
                     AAIIIASSIVLVGVIAAGVIILVNKKSV"
     gene            1697..2182
                     /locus_tag="PLI11_08690"
     CDS             1697..2182
                     /locus_tag="PLI11_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52981.1"
                     /translation="MYKEKTIKWMIIISLILGLIYLFAFTYFIIVQKKLAASYSLHYV
                     DIENVRFVLPFGYVTSILQYIIQATISLVILSKLKDVYSTRAFEIIGIVLFSGAFSFL
                     GRFISNMQFLANKTQAEVLTLNAILMNLDYVSFLAGISVTLFIAACAMSWYGKSYARN
                     H"
     gene            complement(2179..>2561)
                     /locus_tag="PLI11_08695"
     CDS             complement(2179..>2561)
                     /locus_tag="PLI11_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52982.1"
                     /translation="INTFVPTNIMISILFVFSLVACGIGLVVTSYSDSSYMASTLNTL
                     ILTPTCMIGGCFWPVYIMPKFMQNLSYIVPQRWALEAIEKIQKGYDYTAIVLNLFVLL
                     MFAVVFFVIAILRFRKLKSVEKFV"
BASE COUNT          792 a          479 c          596 g          694 t
ORIGIN      
        1 aagcaggcag agatgctggc ggatgctggt attgattttg ttgcatttga ctgtacgaac
       61 ggaagcttta catgggaacc tgcataccta gctgtgtgtg aagtatggtc tgaagcaaga
      121 gaacaaggtg tcaagactcc acagattatt ttcatgctca acttcgggcc gttagctgat
      181 actgttgtat cattgaaatc aatttacaac aggctttaca aaaaagaggc atacaaggat
      241 ttgtggttct actgggaagg caagcccctc attatggcgc atccggagtg ccttaaccag
      301 aacaacgcac tggaaaagga gatctatgag ttcttcacat ggagaagaaa tgtagcttca
      361 tatttcgagg atgacaagga cgacagatac tggggctggc tccatgttta ccctcaagcc
      421 gtctacaaga atgaagacgg taccgttgaa caggttacag ttggcattgc gcagaatgca
      481 gactatgtgg aatggagact ctgtgccatg aatggaccga acaacatggg aagaagctat
      541 accaatggtg aatattccta ttcatacacc tacaggaaca agaccataaa ggttgacaag
      601 aatattgaga attccatgtt ctatggtctt aacttccaac agcagtttga ttttgcaatc
      661 aagcaggatc cggaaattat ttttgtaacg ggctggaatg aatggataat gggcagattc
      721 gatgaatgga tgggtataga gaatgcattc cccgaccagt ttgacgatga gaactcaaga
      781 gacatagagc cttcaaaagg agctctcaaa gactactact attaccagct cattgcaaat
      841 gtaaggaggt tcaagggagc ttccgatatt gattatgcag agaaaggcgt atccatcgac
      901 atatttggag atttgtccca gtgggatgat cctgccattc tgtcctatga tcactataca
      961 aagaacacct acgaaagaaa ctcaagaggc tggggcaggc tcaagtatga gaactacacc
     1021 atgaggaatg acatagtaaa cacaaaggtt acatacgatg atgacaacat atatttctat
     1081 gttagaaccg tggatgatct tacacctcat acggatccgg cctggatgag attgtttttg
     1141 gacacccaga gggcaacaga aacctccgta gactgggaag agtttgaata cgttataaac
     1201 agaaggactc caagcgacag tgttgcatat cttgaaagaa gcaaaggcgg ctgggactgg
     1261 gagcaggttg gcgaagttag atactcggtt aacggcaatg tactgcaaat tgaagtacca
     1321 agagaaatgc tgggcttcaa gaacaagcct atcaacttca acttcaagtg gtcagacaac
     1381 atgcaggaag atggagatat aatggacttc tacctcaatg gcgacgtggc tccaggcgga
     1441 agactctgct ttgcctttag ggacaaggcg gcttatctgg aggaaaaggg agatgagaaa
     1501 gaaggcttta gcattagcac caccgccgca atcattattg catccagtat agttttggtt
     1561 ggtgtaattg ctgctggggt tatcatactt gttaataaaa aatcagttta gggtagacaa
     1621 atatattagt tggatgatga aaacaattca ccattcgcca attgcgaatg gtgaattgta
     1681 ttgaaaggag tatcttatgt ataaagaaaa aactatcaaa tggatgatta ttatctcttt
     1741 aattttgggt ttgatttacc tttttgcatt cacatatttt atcattgtac agaaaaagct
     1801 tgcagcttca tattctcttc attacgtaga tattgaaaat gtgcgctttg ttctgccttt
     1861 tggatatgtg acttccatac tacagtatat tatacaggca accatctctt tggtgatact
     1921 aagcaaactc aaggatgttt actctacaag agcgtttgag ataattggaa tagtattgtt
     1981 cagcggagct ttttccttcc tgggcagatt tataagcaac atgcagtttt tggcaaataa
     2041 aacccaggcg gaagtgctga cgctgaacgc aatattaatg aaccttgatt acgtttcatt
     2101 tcttgccgga atttcagtta cactgttcat tgcagcatgt gccatgagct ggtatggaaa
     2161 atcttacgcc agaaatcatt aaacgaactt ctccacgctc tttaactttc taaaccttag
     2221 aatagcaatc acaaagaata cgactgcaaa catcaaaaga acaaacagat tcaggacgat
     2281 ggccgtatag tcgtatccct tttgtatttt ctcaatggcc tccagcgccc atctctgggg
     2341 tacaatgtag gagaggtttt gcatgaactt tggcatgatg tatacaggcc agaagcatcc
     2401 tccaatcatg caggtaggcg tgagtatcag cgtgtttagc gtgcttgcca tataggagga
     2461 gtcggagtag gatgttacga caagacctat tccacaggcc accagtgaga atacaaagag
     2521 gattgatatc ataatgtttg tcggaacgaa tgtatttatt t
//
LOCUS       DAONVY010000347         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27556_length_2552_cov_3.898278, whole genome shotgun sequence.
ACCESSION   DAONVY010000347 DAONVY010000000
VERSION     DAONVY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27556_length_2552_cov_3.898278"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLI11_08700"
     CDS             <1..509
                     /locus_tag="PLI11_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52983.1"
                     /translation="YRLIFVLTCAYYKDADSFCKKLARRLDRAVFIDTESVEEAKTFI
                     SKTEYEYYIFAAKEVNFVEQFANHNIDVITLTDDSNESVLGIYSQIKLLLMRNIFNAN
                     KNTVKLVVGPVETKSAAERTVSNISSTVRAYLKHELELLGWFYDKKDGIGDIGYRYLE
                     SGGVHVEN"
     gene            496..1239
                     /locus_tag="PLI11_08705"
     CDS             496..1239
                     /locus_tag="PLI11_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPZ52984.1"
                     /translation="MLKTKDESVHMWEKYAKNKKQEDRDALILNYLPLVKKIVYKMMP
                     SYSKYVDLDDLLGSGIMGLIHAVDRFDPGKGVPFESFAYLRIKGEIIDYLRRQDFVST
                     SLRKRIKNVESAMESLEIQLGRPPTDEELAKHLNMDQETLQKTLDDAYTYNVMYLEDV
                     LEKCVSLPSESKSELPEESYDELELKEILAKGIDSLSDREKLVISLYYYDDLSIKEIG
                     HVLGVSESRVSQIHSKALMKLRGKIDWMK"
     gene            1227..1793
                     /locus_tag="PLI11_08710"
     CDS             1227..1793
                     /locus_tag="PLI11_08710"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPZ52985.1"
                     /translation="MDEINRALVIIKESISDFDDTTRAHSRRMMILSRGMVETIGLSE
                     SDGDVLELTCAIHDIGKRFIPACILNKKGRLTEEEWAEIKTHPLRGWELLSQYAFLRD
                     VAEAILHHHERWDGTGYPYGRKMEAIPVIARIVSVIDAFDAMTNARPYNLVMSVNDAL
                     AELGRHAGTQFDPDICKRFVRMYKESFF"
     gene            2234..>2552
                     /locus_tag="PLI11_08715"
     CDS             2234..>2552
                     /locus_tag="PLI11_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52986.1"
                     /translation="MTSRCYLRGDNVEEFVLPLIAISMYYMLDMYLNGERGRLYNLWM
                     MFLNGIFVGFVFWLKFNLLGFWIGFFLFSLVRLLKHKRFGEFFTSAIAVLAGFVLVTF
                     PVLL"
BASE COUNT          762 a          429 c          593 g          768 t
ORIGIN      
        1 attacagact gatatttgtc ctgacatgcg cctattacaa ggatgcggat tccttctgca
       61 aaaaacttgc caggcgtttg gatagagctg tttttattga cactgaaagt gttgaggaag
      121 caaaaacatt tatttcaaaa acagaatacg aatactatat ctttgccgca aaagaagtaa
      181 actttgtgga gcagtttgca aatcacaata ttgatgtaat taccctgacc gacgattcca
      241 acgaaagtgt tttgggcatt tactctcaaa tcaaattgct tcttatgcgc aatatattca
      301 atgcaaacaa aaacactgtc aaactggttg taggaccggt ggaaaccaaa tcagcagcag
      361 aaagaaccgt atctaatatt tcaagtacgg taagggccta ccttaaacac gaacttgagt
      421 tgttgggctg gttttatgac aagaaagatg gtattggtga tataggttat aggtatctcg
      481 agtctggagg agtacatgtt gaaaactaaa gatgagtctg tacatatgtg ggagaaatat
      541 gctaaaaaca aaaaacagga agacagggat gccctgatac tgaattatct gcctcttgtg
      601 aaaaagatag tatacaaaat gatgcccagt tacagtaaat acgtggatct ggatgacctt
      661 ctgggaagcg gcataatggg acttatacat gcggtagaca gatttgaccc aggcaagggg
      721 gtgccgtttg aaagctttgc atatcttcgt ataaaagggg aaataataga ttatctcagg
      781 agacaggatt ttgtttccac aagccttagg aagagaataa agaatgttga gagcgccatg
      841 gagagcttgg agatacagct tggtcgtcct cccaccgatg aggagctggc taaacacctg
      901 aatatggacc aggagacatt gcaaaagacc cttgatgatg cttacacata caatgtaatg
      961 tatcttgagg atgtgcttga gaaatgtgtg tctctgcctt ccgagagtaa aagcgaactg
     1021 ccggaagaaa gttatgatga gctggaactc aaggaaatac tggctaaagg tattgatagc
     1081 ctttccgaca gggaaaagct tgtaatctcc ctctactatt acgatgacct aagtatcaag
     1141 gagattgggc atgttttggg tgtcagcgaa tccagggtat ctcagattca ctccaaggcg
     1201 ctaatgaaac ttagggggaa gattgattgg atgaaataaa cagggccctt gtaattatta
     1261 aagagtcgat atccgacttt gacgatacaa caagagctca ctcaagacga atgatgatcc
     1321 tttcaagagg catggttgag actatagggc tatccgaatc cgatggagat gtgcttgaac
     1381 ttacttgtgc aattcatgat attggaaaga gatttattcc agcttgcatt ctaaacaaga
     1441 aaggcagact tacagaagag gaatgggcag agataaagac ccatccgtta agaggatggg
     1501 agctgctaag ccagtatgct tttcttcgtg atgtggcgga agcgattctg catcatcatg
     1561 agagatggga cggaactggc tatccttatg gcagaaagat ggaagcgatt cctgttattg
     1621 caaggattgt ttcagtcata gatgcctttg atgctatgac aaacgcaaga ccatacaacc
     1681 tggttatgag tgttaatgat gctcttgctg agctaggcag gcatgcaggc acgcagtttg
     1741 acccggatat ttgcaaacgt tttgtccgga tgtacaagga gagttttttt taacaccaat
     1801 cttggcaaaa tatgtactag ctggtataat gagctgccag attattatag gtttgaaaga
     1861 aggactatgg tttatgagat ttaaaagctt tgcttttata cttgttctag cgtttttttt
     1921 actgactatt tgttccatga catcatttct ttacccgata cataacaggg tggatcagaa
     1981 ttgctttatg acaatcggca agggaatttt aaaagggcag gtaccataca gagacttgtt
     2041 tgatcaaaaa gggcctgtta ttttttttat gcatgcatta gcttcccgca tctccactac
     2101 atcgtttatt ggagtttatt ttttacaata tgtttttctc attgtattca tgatttacac
     2161 aagaaaaaca gcatgtttgt ttgtgcctga gaaaaaggcc aattttattg tcttttttac
     2221 tctggggctt atcatgacat ccagatgtta tttaagggga gacaatgtgg aagagtttgt
     2281 acttcctctt attgcaatat ccatgtatta catgctggat atgtatctca acggggagag
     2341 gggaagatta tataatcttt ggatgatgtt tttaaatggt atttttgttg gttttgtgtt
     2401 ctggctcaag tttaatctgc tgggtttttg gattggcttc tttttattct cgcttgtacg
     2461 gcttctcaaa cacaagagat ttggagagtt tttcacatcc gcaattgcgg tattggcagg
     2521 ctttgtatta gtcacatttc cagtactgtt at
//
LOCUS       DAONVY010000348         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27594_length_2549_cov_3.275862, whole genome shotgun sequence.
ACCESSION   DAONVY010000348 DAONVY010000000
VERSION     DAONVY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27594_length_2549_cov_3.275862"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLI11_08720"
     CDS             complement(<1..354)
                     /locus_tag="PLI11_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ52987.1"
                     /translation="MKVLKVIIPIVLILSLSSCMINSEEDVENTEAIISTPSSEPTST
                     PSPTPTEEPVKDRIIEMVEKMTIEQKIGQLVITGYGSVDEVINSNIAFGGVILFGRNI
                     KSVSETKSDIARLQEN"
     gene            673..939
                     /locus_tag="PLI11_08725"
     CDS             673..939
                     /locus_tag="PLI11_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HPZ52988.1"
                     /translation="MAYKNAKDYLPKELLSEVQKHIQGVLVYIPTDDSAKKGWGKLSG
                     TRKELDKRNASIREDYKQGLTVDEIADKYYLSVDSIRKIVYGKK"
     gene            complement(966..1442)
                     /locus_tag="PLI11_08730"
     CDS             complement(966..1442)
                     /locus_tag="PLI11_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ52989.1"
                     /translation="MEISVRRAEEKDIDRIVALLKVIANEHHKGRPDLFREGRKKYSA
                     HEVRDILYEEDRAVFVAVDENDYVLGYLFSYIIDQRTASLFRNFKTLYIDDLCIDENF
                     RNTGIGKKLFAHSVEFARECGCYNIELNVWSFNDRAIEFYKKQGMEIQCYKMEYRL"
     gene            complement(1464..>2549)
                     /locus_tag="PLI11_08735"
     CDS             complement(1464..>2549)
                     /locus_tag="PLI11_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPZ52990.1"
                     /translation="KHYTEELKKVSRDMPLRYFTQMNWDLVGSPQLLEEPQENPLKRY
                     KWKKADRTVVTSSPMMGYFFAAKMLELNPDVPIGIVNVACGGASLAHLAPKEVVDKFP
                     AGMKNKQLPINTLVVKDSGIYNAFMHPFIDFGIKGMLFYQGESDTVSYKEYPEALNTY
                     VQDLRDRFGEHFYFLNVQLSSYGYYAGNIPLTGIWDQINDLRNAQAKVRIDDIMYNYE
                     IVPSLDCGWQEGDSDGAHPIYKKPICDRLANIAAALVYGIGDMENAACPVPKDVRFES
                     DKVVIEFMYAGGGLKVNGDKLNGFEVLINGKWDKIDAGIEGNKVIINGDNIEGVRYGY
                     DLRIEDPSQITLVSGTDNPAVAFEVLK"
BASE COUNT          749 a          568 c          443 g          789 t
ORIGIN      
        1 attttcttgc agtcttgcaa tatcactctt tgtctccgac actgatttta tgttcctgcc
       61 aaaaagaatt actccgccaa aagctatatt tgaatttatg acctcatcca cactgccata
      121 cccggttatg acaagctggc caatcttctg ctctatagtc attttttcaa ccatctctat
      181 aatcctgtcc ttcacgggtt cttctgtcgg agttggcgaa ggagtactgg taggttctga
      241 ggaaggtgtt gagattatag cttcagtgtt ttccacatct tcttctgaat taatcataca
      301 tgatgaaaga ctcaatatga gcactatcgg gattataact ttaagaactt tcaaaaaatc
      361 accttcaatt tttcttttaa ttataccatg ataaattata accattcttt acattgattt
      421 atttacttac tgatatataa ttactataca gcctaacatt ggcgagttcc gaaggaaggg
      481 agctgataaa agtgattaag ttgaggtggt ctttgttcta gaccttaaac ggaacaaaga
      541 ataaaggtga aatccgggcg gtgaaaatac cgcctgggtt ttgccgtatt taaaaacata
      601 gaaagggggt aatcagaatg gatattggct tttacaaacc tgtacaagtt tttgtaaagg
      661 aggaatattt aattggctta caagaatgcg aaagattatc tcccgaagga gctcctttct
      721 gaagttcaaa aacacattca aggagtgcta gtatacattc ctactgacga ttctgctaaa
      781 aaaggttggg gaaaactctc gggaacaaga aaagaacttg ataaaagaaa tgcatcaata
      841 cgagaagact acaaacaagg tcttacagtt gatgaaatag cggacaaata ttatttatcc
      901 gtcgacagca taagaaaaat tgtatatgga aaaaagtgaa gctctccgac actggagagc
      961 tccttttata acctgtattc cattttgtag cactgtattt ccatgccctg ttttttgtaa
     1021 aactctattg ccctatcatt gaaactccat acgttgagct caatgttgta acatccacac
     1081 tctcgtgcaa attctacact gtgggcaaag agcttcttgc cgattccagt atttctgaaa
     1141 ttttcatcga tacacaaatc gtcaatatac agtgttttaa agtttctaaa aagggatgcc
     1201 gttctctggt caatgatata gctgaacagg tatccaagga catagtcatt ctcgtccact
     1261 gcaacgaata ccgccctgtc ctcttcataa aggatgtctc taacctcgtg agcagaatac
     1321 ttctttcgac cttctctgaa aagatctggc cttcctttat gatgttcatt ggcaataact
     1381 ttgagtaatg caactatcct gtctatatcc ttctcttctg cccttctaac gctaatctcc
     1441 ataacgcaac cctcaaatcc acattatttc aatacttcaa aagcaacggc aggattgtca
     1501 gtaccggaca ccaaagttat ctgggacgga tcctcaattc taaggtcata tccgtagcgg
     1561 acgccttcaa tgttgtcacc attgattata accttgttgc cttcaatccc ggcatctatt
     1621 ttatcccatt ttccattaat caatacttca aagccattca gcttgtcacc atttaccttc
     1681 aagccgccgc ctgcatacat gaactcaatt acaaccttgt cactttcaaa acggacatcc
     1741 tttggaacag gacatgcagc gttctccata tctccaatac catacacaag agctgctgct
     1801 atgtttgcaa gcctgtcgca tatcggcttt ttgtagatag gatgagcacc gtcgctgtct
     1861 ccttcctgcc aaccgcagtc caaagacgga actatctcat agttgtacat aatgtcatca
     1921 atcctaactt tagcctgagc attcctcaaa tcatttattt gatcccatat tcctgttagc
     1981 ggtatgttgc cagcataata accgtatgag gagagctgca cattcagaaa gtaaaaatgt
     2041 tctccaaacc tgtctctcaa atcctgcaca taagtgttca gtgcttcagg atactccttg
     2101 tagctgacgg tatcagattc tccctgatag aaaagcattc cctttatgcc aaaatcaata
     2161 aacggatgca tgaatgcatt gtatattcct gaatccttta caaccagcgt gttaattggc
     2221 aactgtttgt tcttcatgcc tgcagggaat ttgtctacaa cctctttcgg tgcaagatgt
     2281 gcaagagaag caccaccaca agcaacatta acaattccta ttggcacatc cggatttaat
     2341 tcaagcatct tggctgcaaa gaaatacccc atcatcggag aacttgtaac aacagtccta
     2401 tcagcttttt tccatttgta cctttttaaa ggattctcct gtggctcttc taataattgg
     2461 ggagagccaa caagatccca gttcatttga gtgaaatacc taagtggcat gtctcttgat
     2521 acttttttaa gttcttccgt atagtgctt
//
LOCUS       DAONVY010000349         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27597_length_2549_cov_2.817562, whole genome shotgun sequence.
ACCESSION   DAONVY010000349 DAONVY010000000
VERSION     DAONVY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27597_length_2549_cov_2.817562"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLI11_08740"
     CDS             complement(<1..524)
                     /locus_tag="PLI11_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ52991.1"
                     /translation="MITIYKREVAAFFRSPIAYSVIGFFMVLTGLFFWINNIIIGSTE
                     FSETLSTISLFLIFLAPVITMKLLADEKRNGTDVLLRTSPITVPQIIVGKYLAALTVF
                     SVMIVLSFIYPIIISILGDIPMAMTVGGYIGFFVMGASFLAVGLFTSSLTENQIVAAV
                     SGIVILLCMWFLQS"
     gene            complement(521..1471)
                     /locus_tag="PLI11_08745"
     CDS             complement(521..1471)
                     /locus_tag="PLI11_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPZ52992.1"
                     /translation="MIEVKNLTKVYGSIVALDNVSFNVRKGEILGFLGPNGAGKTTAM
                     NIITGYLPSTSGTVLVQGHDIMEEPKEVKRLIGYLPEQPPLYTDMTVREYLNFICDLK
                     GVELRKRKSQLDDIMYLVKIGDMRKRLIKNLSKGYRQRVGLAQALVGDPPVLILDEPT
                     VGLDPKQIIEIRKLIMALGRDHTVILSTHILSEVSAVCSDVIIINKGKIAAAGSVSNF
                     SATIGSSNKFMVHIIGSKNDILKKINGINNIKYVDFLRVDGDVNMFLVESKTDIRREL
                     FNEMSKANYPIIELRPVGKTIEDIFLDVVSKDTPVREEIR"
     gene            complement(1559..>2549)
                     /locus_tag="PLI11_08750"
     CDS             complement(1559..>2549)
                     /locus_tag="PLI11_08750"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPZ52993.1"
                     /translation="CVVLFGLLYFNLLLPLWNFLNNELENPNDIKPGTSIGAGIKIPT
                     PNPSQDPSESEEPKPSIPNAEGDLNILLLGADDAGGLTDTICIVNIDKNNKKIKLISI
                     PRDAYIPYEDWVKEKLAEEGLLHAKGIFKINMTAYLGRLVGYPHGKFENKGINFMSDI
                     IESMLGYRIDEYMHINFDGFVELVDLFGGIKVTAPENIYNNKGELIIKEGLNELDGKK
                     ALFYARARYRYDEFGNQLPSPGDSYRKTNQLNMIVEVSKQLVTPENITKAQDILNTLR
                     KNLHHSITVSDLSEYSKIALDYAMGKYTIETHVIRGVPFFPDDDPTVEYVSIL"
BASE COUNT          730 a          568 c          464 g          787 t
ORIGIN      
        1 attgactgta aaaaccacat acaaagcaga ataactatac cggaaaccgc cgctactatc
       61 tgattctctg tcagtgaaga tgtaaaaagt cccactgcaa gaaaagacgc tcccatgaca
      121 aagaatccga tatatccgcc aacagtcatc gccatcggaa tatctcccag aatgctgata
      181 attattgggt aaatgaaaga aagcacaatc ataacagaaa atactgtcaa cgctgcaaga
      241 tacttgccaa cgattatctg tggcacggtt atcggagatg ttctcaaaag aacatcggta
      301 ccgtttctct tctcatcagc caataatttc atggttatga caggcgccag gaatatgaga
      361 aatagtgaaa ttgtactcag agtttctgaa aattccgtac tgccgataat tatgttgttt
      421 atccagaaaa acaaaccggt tagtaccatg aagaaaccaa ttacactata tgcaatggga
      481 gatctgaaaa atgcagcaac ttcccttttg taaatcgtaa tcatctaatc tcctccctta
      541 caggcgtatc ttttgataca acgtccagga atatgtcctc aattgtcttg ccaacaggtc
      601 tgagctcgat aattgggtag tttgccttgg acatttcatt aaacagttcc cttctgatat
      661 ccgtcttcga ctcaacgaga aacatgttca catccccatc gacacgcaga aaatcaacgt
      721 attttatgtt gttgattcca tttatcttct ttaatatatc atttttggaa ccaataatat
      781 gcaccataaa cttgttgctg gatccgattg tggctgaaaa attggaaaca gaacctgctg
      841 ccgcaatttt tcccttgtta atgattatta catccgagca gactgcactg acttcggata
      901 atatgtgagt gctcagtata acggtatgat cccttcccag ggccattatg agctttctaa
      961 tttcaataat ctgttttggg tcaagcccta cggtgggctc gtccagaata agtacaggag
     1021 gatctccaac caacgcttgt gcaagaccta cacgctgtct atatcctttt gaaaggtttt
     1081 tgattagtct ttttctcatg tcccctattt taacaagata catgatgtcg tcaagctgcg
     1141 actttctctt tctaagctcc acgcctttca agtcacaaat aaaattcaaa tactccctga
     1201 ccgtcatgtc agtatacaaa ggtggctgct cgggcagata gccaataagc ctttttactt
     1261 cctttggttc ttccattatg tcatggcctt gtacaagcac tgtaccagat gtggatggta
     1321 gatatccagt aattatgttc atagctgtgg ttttgcctgc accattgggc cccaaaaagc
     1381 ctaatatctc acccttgcgc acgttgaagc ttacattgtc aagcgcaaca atagagccat
     1441 agaccttggt gagattttta acttctatca tcaattctcc tccttacaaa acatctctat
     1501 taaattatat tacatttgat aattttagca aataaattaa ttctaaacaa tctataaatc
     1561 aaagaatgct gacatattca accgtagggt cgtcatcagg aaagaagggt actcccctga
     1621 ttacatgcgt ttctattgtg tatttgccca tggcgtagtc aagtgctatc ttggaatact
     1681 cggacaaatc cgaaacggta atgctgtgat gaaggttttt gcgcaaagtg ttcaatatat
     1741 cctgagcctt ggttatattc tccggtgtaa caagctgctt tgacacctcc actatcatgt
     1801 tgagctggtt tgtctttctg taagagtctc cagggcttgg cagctggttt ccaaactcat
     1861 cgtaacgata tcttgccctt gcatagaaca gtgccttttt tccgtccagc tcgttaagac
     1921 cttccttgat tattagctcg cccttgttgt tgtaaatatt ctcaggtgcg gtaaccttta
     1981 tgccaccaaa caaatccacc agctctacaa agccgtcgaa attgatatgc atgtactcat
     2041 caattctgta acccagcatg ctttcaataa tatcgctcat gaaatttatg cccttgtttt
     2101 caaactttcc atgaggatat cctacaagcc ttcccaaata agcggtcatg ttgattttaa
     2161 aaataccctt tgcatgcagc aatccttctt cggccagctt ttccttaacc cagtcctcgt
     2221 aaggaatata tgcatccctg ggtatgctga taagctttat ctttttgtta tttttgtcaa
     2281 tattgacaat acaaatggtg tcagtaagac cgccggcatc gtccgcacca agaagaagga
     2341 tgttcaaatc tccttctgca tttggaatgg acggttttgg ttcttccgat tctgatgggt
     2401 cctgtgaagg atttggcgtt ggaattttaa ttccagcgcc aatactagtt ccaggtttga
     2461 tatcatttgg attttcaagt tcgttgttta aaaagttcca caaaggaagc agtaagttga
     2521 aatacaaaag tccaaacagt accacgcag
//
LOCUS       DAONVY010000350         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27778_length_2535_cov_5.008871, whole genome shotgun sequence.
ACCESSION   DAONVY010000350 DAONVY010000000
VERSION     DAONVY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27778_length_2535_cov_5.008871"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLI11_08755"
     CDS             <1..325
                     /locus_tag="PLI11_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPZ52994.1"
                     /translation="RFRSENQQQTADFINCVAWRSTAEFISKYFAKGSKMAVSGRIET
                     SSWEDQEGKKRYSTDVVIDEVEFADSKRQDGPVNDSMPGYGAESQPQQAGFFVLDEDD
                     GDIPF"
     gene            362..634
                     /gene="rpsR"
                     /locus_tag="PLI11_08760"
     CDS             362..634
                     /gene="rpsR"
                     /locus_tag="PLI11_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPZ52995.1"
                     /translation="MVKKESNKDSEKEVKNFTRQRRGRKKVCPFCVDKVEHIDYKDIA
                     KLRKFVSERGKILPKRISGNCAKHQRQLTNAIKRARHVALMPFSSD"
     gene            704..937
                     /locus_tag="PLI11_08765"
     CDS             704..937
                     /locus_tag="PLI11_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HPZ52996.1"
                     /translation="MLNVFGNVRQIDNCKADLVMAVGKLYKDLSADEIIIDEVNEDIS
                     ELIVLSYALGKKLGLDQDEISNKVIRKLRLRKV"
     gene            939..>2535
                     /locus_tag="PLI11_08770"
     CDS             939..>2535
                     /locus_tag="PLI11_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPZ52997.1"
                     /translation="MKGKFSIRKVNMALINFVIAAALIANSIYFYAVQKSYRIVLVYI
                     LLLVAVIILNIVIAILKRKAIVKIVSGISFHLKEGKEGSIRDFHLPLLFINESGELIW
                     HNKHFANIFEKKKTIRKTVNELFLLKLKSKLIENDTNLMSEVYLEGRTYQMLGNIIKA
                     EKEEKRGFYIMLYFIDITEMVKLRQKYEDEKLAVGIIVIDSYEEIYRSSGEAVVNEIF
                     VELNRIFDEWLKGKGAIIRRLIRDRYVLLAENHELKEFEKNKFEILDKAKNINVGNKI
                     PVSLSIGIAKDGQTIEENYKNAEMAVELALGRGGDQAIVRSNNKDTYYGGSNIEMERR
                     TKVRARVISNLLKEEIDKSSRVIIMGHNNCDMDSLGASLALYRACCLRNLKANIILNS
                     VNQSIAAIMEKLSTMEEYEEVFINSSYALNIIDDNTLVIVVDTYNKELTECPKLLDYA
                     SRIALIDHHRRGSDFIKNTVIDYAETYASSTSELLVEILMYFEPNVTIPPIEAEALYA
                     GILVDTKNFTFKTGTRTFEAASFL"
BASE COUNT          895 a          400 c          581 g          659 t
ORIGIN      
        1 cagattcagg tcggaaaacc aacagcagac cgcagatttt ataaactgcg ttgcgtggag
       61 aagtactgct gaattcatat cgaaatattt tgccaaaggt tctaaaatgg ccgtatctgg
      121 cagaattgag acgagttcct gggaagatca ggaaggcaag aaacgctact ccaccgatgt
      181 tgttatcgat gaagttgagt ttgcagacag taaacgtcag gatggacctg taaatgacag
      241 catgcctgga tatggcgctg aatctcaacc tcaacaggct ggattctttg tgctggacga
      301 agatgatgga gatataccat tctaagtaat attacctatc tagaaagaag gaggcgaaaa
      361 catggtgaag aaagaaagca acaaggatag cgaaaaggaa gtaaagaact ttacaagaca
      421 gagaagagga agaaaaaagg tttgtccttt ctgcgttgat aaagttgaac atattgatta
      481 taaagatata gctaagctta gaaagtttgt ttccgaaaga ggcaagattc tgccaaaaag
      541 aatttctgga aactgcgcaa agcatcagag acagcttacc aacgctataa agagggcaag
      601 acacgttgcg ttgatgccat tttcttctga ttaatataag taaaaagcgc agcttccata
      661 gttgcgctct ttttgtatat taaaaacgga atgaggggtt tgtatgttga atgtttttgg
      721 taatgtcaga cagattgaca attgtaaagc cgacttggta atggcagtag gaaaactata
      781 caaagacctt tcagccgacg aaataataat tgatgaagtg aacgaggaca tatctgagct
      841 aatagttctt tcttatgcac tcggaaagaa actgggactt gatcaggatg aaatcagcaa
      901 caaggtaata agaaaattaa ggttgagaaa agtataacat gaaaggtaaa ttttcaatcc
      961 gaaaagtaaa tatggcgctg atcaactttg tgatagctgc agcactgatt gcaaacagca
     1021 tttattttta tgcagtgcag aaaagttaca ggatcgtgct ggtttatatt ttgctgcttg
     1081 tcgctgtcat tatattaaac attgttatcg ccatactgaa aagaaaagcc atagtaaaaa
     1141 ttgtgtcggg catatctttc cacttgaaag aaggaaagga aggttcgata agggatttcc
     1201 atttgcctct cttgtttata aatgagagcg gggagcttat ctggcacaat aaacattttg
     1261 ccaatatttt tgaaaagaag aagacaatta ggaagactgt caacgagctg tttcttttga
     1321 aattaaaaag caagcttata gagaacgata cgaaccttat gagcgaggta tatcttgaag
     1381 gcagaacgta tcagatgctg ggcaatatta tcaaagctga aaaagaagaa aaacgcggct
     1441 tttacatcat gctgtatttc atagatatta ccgagatggt gaagcttagg cagaagtacg
     1501 aagatgagaa acttgcagta ggaataattg taattgatag ttatgaagaa atatatcgtt
     1561 ccagtggtga agctgtagta aatgaaattt ttgtagaact taacagaata tttgacgaat
     1621 ggttgaaagg aaaaggcgca atcataagaa gattgataag agacagatat gtgctgcttg
     1681 ctgaaaacca tgagttgaaa gagtttgaaa agaacaagtt tgaaatattg gacaaggcaa
     1741 aaaatattaa cgtaggaaac aagatcccgg tgtctcttag cataggtatt gctaaagacg
     1801 ggcaaacaat agaagagaac tacaaaaatg cagaaatggc tgtagaactt gcacttggaa
     1861 gaggcggtga ccaggccata gtcaggtcaa acaacaagga tacctactat ggcggaagca
     1921 atatagaaat ggagcgccgt acaaaggtaa gggcaagggt aatatcgaat cttctgaagg
     1981 aagagataga caagagcagc agggtaatca ttatgggcca taataactgt gacatggaca
     2041 gtttgggagc ttctcttgca ttatacaggg cttgctgctt gagaaactta aaagccaata
     2101 ttattcttaa ctctgttaat cagtccattg cagctataat ggaaaaactg tccacgatgg
     2161 aagaatacga ggaggtattc attaactcca gttatgccct gaacataatt gacgacaata
     2221 ctcttgtcat agttgtggac acttacaaca aggagcttac agagtgtccg aagcttcttg
     2281 attatgcaag cagaatcgca ttaattgatc accatagaag aggttccgac ttcataaaaa
     2341 atacggtgat agattatgcg gaaacatatg catcatctac cagcgaactg ctggttgaaa
     2401 tacttatgta ttttgaaccg aatgtgacaa taccacccat agaggcggaa gcgctttatg
     2461 caggaattct tgtagatacc aagaacttta ccttcaaaac aggcacgaga acctttgaag
     2521 ctgcttcctt tttaa
//
LOCUS       DAONVY010000351         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27875_length_2528_cov_3.285888, whole genome shotgun sequence.
ACCESSION   DAONVY010000351 DAONVY010000000
VERSION     DAONVY010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27875_length_2528_cov_3.285888"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLI11_08775"
     CDS             <1..1192
                     /locus_tag="PLI11_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPZ52998.1"
                     /translation="ANVTKKETSFADFTVKGSQFNQPLLEFSGSCAGCAETSYARLIT
                     QLFGERMYIANATGCSSIWGGSAPATPYTVNRETGRGPAWANSLFEDNAEFGYGIYLG
                     QRTLRERLVGYVEELLEKTTDEADRAVIKEYLDTKNDGQANFKATEKLVKVLEKNTDD
                     LSKEILENKNYLAKKSVWIFGGDGWAYDIGYGGLDHVIASGEDVNIFVFDTEVYSNTG
                     GQASKASQLGQVAQFAAAGKDIGKKDLARIAMTYGYVYVAQIAMGANLNQTLKALQEA
                     EAYPGPSIIIAYSPCEMHGIKGGMGNSQIRMKEAVESGYWTLFRYNPMAIAEGKNPFT
                     LDSQAPKKSYRDFIMSETRYTRLNQTFPERADELFGKAEEAAADKYELIQRLKDFYEP
                     NKEE"
     gene            complement(1357..>2528)
                     /locus_tag="PLI11_08780"
     CDS             complement(1357..>2528)
                     /locus_tag="PLI11_08780"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar hydrolase"
                     /protein_id="HPZ52999.1"
                     /translation="NIDVLAEPFTLPRRYAFRYLKVEILGRCTAYRVKFEDIYCTAVT
                     SSDCSSIEKLNDDMDTMLAKIDEVSIRTLKNCSQEVYEDGPKRDRRLWLGDLRLQAIA
                     DYVTFKNYNLVKRCLYLFAGLPHPDGQISSCIFHEPTLSNDSWILNDYSLFFISVLYD
                     YFNETNDLNLLKELWDTAFRQAEIVSEQIDEYGLVKHGQTKYFVDWCEGLDKNTSAQA
                     IAIYTFKQCHALATALNDEKRMSFLEENINVLTKGAVKHLYNNEIGFFESGERNQLSW
                     HSQIWMILAGVFEKDANANLLKRLINSDIEIRPRTPYMYHHFIQALFDCDMKEDAIKY
                     IKAYWGKMVELCADCFWEVFDPDNLLLSPYNSIQINSYCHAWSCTPSYFIRKYLS"
BASE COUNT          734 a          530 c          563 g          701 t
ORIGIN      
        1 tgcaaacgtt acaaagaagg aaacttcctt tgcagacttt acagtcaagg gaagccagtt
       61 caaccagcca ttgcttgagt tctcaggctc ctgcgcaggt tgtgccgaga cctcctatgc
      121 acgcctcatc acccagctgt ttggtgagag aatgtacata gcaaacgcaa caggatgctc
      181 atccatctgg ggcggctctg cacctgctac accttacaca gtcaaccgtg agacaggaag
      241 aggtcctgca tgggcaaaca gcttgttcga agacaatgct gagttcggct acggtatcta
      301 ccttggtcag aggaccttaa gagaaagact cgttggttac gttgaggaac tccttgagaa
      361 gacaactgac gaagccgata gagctgttat caaggaatac cttgatacaa agaatgatgg
      421 tcaggctaac ttcaaggcta ccgaaaagct tgtcaaggtt ctcgagaaaa atacagatga
      481 cctttccaag gagattcttg aaaataaaaa ctatctggct aagaagtcag tatggatctt
      541 cggtggtgac ggatgggctt acgatatcgg atacggcggt cttgaccatg taattgcttc
      601 tggtgaagat gtaaacattt tcgtatttga tacagaagtt tactccaata ccggcggaca
      661 ggcatccaag gcttcacagc tgggtcaggt tgcacagttt gctgcagctg gtaaggatat
      721 cggcaagaaa gatcttgcac gtattgcaat gacctacggt tatgtctacg tagctcagat
      781 tgctatggga gcaaacctga atcagacttt gaaagctctc caggaagctg aagcataccc
      841 aggaccatcc attatcattg catactcacc atgtgagatg cacggtatca agggcggcat
      901 gggcaactca cagattcgca tgaaggaagc agttgaatct ggctactgga cactcttcag
      961 atacaatcca atggctattg cagagggcaa gaatccattt actttggata gccaggctcc
     1021 aaagaagagt tacagagact ttataatgag tgaaacccgt tacacaagac tcaatcagac
     1081 cttccctgag agagcagacg agctctttgg aaaggctgag gaagcagcag ctgacaaata
     1141 tgaacttatc cagaggctga aggatttcta tgagcctaat aaggaagagt aaatattacc
     1201 aaatagataa attatggagc agtctcattt gtgggactgc tcctttttga atatgcagaa
     1261 tctttttctt ggtgttaatt ggactggcgg gtgttaatgg caacaaggga caaggacaaa
     1321 aacatgaaaa aactgcggaa aatcccgcag taattattag cttaagtatt ttcgtatgaa
     1381 ataactggga gtgcagctcc aggcgtggca gtaactgttt atctggattg agttatacgg
     1441 ggagagcaac aggttgtccg ggtcaaacac ttcccaaaaa cagtcggcgc acagctcaac
     1501 cattttaccc cagtatgcct tgatatattt tatagcatct tccttcatat cgcagtcaaa
     1561 gagagcctgt atgaaatgat gatacatgta aggagtgcga gggcgaatct caatgtctga
     1621 gtttattagt ctttttaaaa ggtttgcgtt tgcatccttt tcaaaaactc ctgctagaat
     1681 catccatatt tgagagtgcc aggacaattg atttctttca ccgctttcaa agaagccaat
     1741 ctcgttattg tataaatgtt tgaccgcacc tttggttaag acatttatat tttcttcaag
     1801 gaagctcatg cgtttttcat cattcaatgc tgttgcaaga gcatggcact gcttgaaggt
     1861 gtatattgcg atagcttgag cggatgtgtt tttatcaagt ccttcgcacc agtctacaaa
     1921 gtactttgtc tgaccgtgct tgaccagtcc atattcatca atttgctctg agacaatttc
     1981 tgcttgccgg aaagcagtat cccaaagctc tttaagcaaa ttcaaatcat ttgtttcatt
     2041 gaagtagtcg tatagtacgc ttatgaaaaa cagtgaatag tcgtttaata tccagctgtc
     2101 gtttgacaaa gttggctcgt gaaaaataca gctgcttatc tgtccgtccg gatgtggaag
     2161 accagcaaac aaatataggc atctttttac cagattgtag tttttaaagg ttacatagtc
     2221 ggcaatcgcc tgcaatctca ggtctcctag ccagagcctt ctgtcgcgct tgggaccatc
     2281 ttcatagact tcctggctgc agtttttaag agtccttata gacacttcgt ctatttttgc
     2341 aagcattgta tccatatcat cgttcaactt ttcaatactt gagcaatcgc tggaagtaac
     2401 cgcagtacag tatatgtctt cgaacttaac cctgtacgca gtacaccgtc ccaatatttc
     2461 aacctttaag tatctgaaag cgtatcttct aggaagagta aaaggttctg ccaaaacatc
     2521 aatattta
//
LOCUS       DAONVY010000352         2521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_27977_length_2521_cov_3.143552, whole genome shotgun sequence.
ACCESSION   DAONVY010000352 DAONVY010000000
VERSION     DAONVY010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27977_length_2521_cov_3.143552"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2021)
                     /locus_tag="PLI11_08785"
     CDS             complement(24..2021)
                     /locus_tag="PLI11_08785"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HPZ53000.1"
                     /translation="MTEIRFEELKIPAAGLHGENPLPDIKGLRQRLGEIKFSDEIRED
                     EKEYFYYGRIKSILPYCIQDDYDRAMKTRAFKACVLENEYLRATFLPELGGRLYSLYD
                     KENKRDLVHRNPVFQPANLALRNAWFSGGVEFNIGMTGHTPFTVSPLFTAVGTMKDGT
                     KFLRMYEWERIRKLAYSIDAYLPEKSRFLFVRITVINDNEETVPMYWWSNIAVDETEK
                     TRIIVPCEKAYVQYADGSFGKDPVPMLGEQDRSYPARIPYAFDQFFDIPKEERKWICA
                     LDGDGKGLVHTSTDRLLGRKLFNWGDSRGGRRWQEFLSVKGSSYCEIQAGCAHMQQQR
                     TPMEGNSVWNWTEAYGYLEADKDIVHGDDWTKVYKCVGEELEKRLPRKNLDEIHESFS
                     IDNVELVHEGSGWGALELKRRERGKVLHGLVFDNIGKEQEIWLRLLEENVFEEFPVNE
                     EPAKYVTQVEWMNLLEKSTKENPKSFNWHALYHLGVMKAANEDLDGALECFQKSIDLK
                     PNIWSYWCLGILENNVEYMDKALGMLTGMTKIDVRIVVDCLNMCNKKGLYEKALKVVY
                     SLDESITGHPRVRLACIDSLIGVNEFDKAEKMFSEDIVPADLKEGEELLSNQWYKLHA
                     RKIMHDEGIGEEKYKEVLKRVVEEVKLPDYLDFIMQYRPLT"
     gene            2151..>2521
                     /locus_tag="PLI11_08790"
     CDS             2151..>2521
                     /locus_tag="PLI11_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53001.1"
                     /translation="MSNRFYGLTLLALLLVMTLIVITGCKAQDDTNPTPTPTNNTIHN
                     TPGETENTSTPSETTGSTSTPMPTETETQTATPGPTETNAPATDTPTPKPTPTPTPTP
                     TPKLEVTFSHEGGFYDNNFKL"
BASE COUNT          725 a          687 c          364 g          745 t
ORIGIN      
        1 tatatgatac cagaatcaat ttatcaggtc aacggcctgt actgcatgat aaagtcaaga
       61 taatcaggca gctttacttc ctcaaccacc cttttcagta cctctttgta cttttcttca
      121 ccaattcctt catcatgcat tatctttctt gcatgtagct tgtaccattg atttgacaaa
      181 agctcttctc cttcctttaa atctgccgga acaatatctt cactaaacat cttttcagcc
      241 ttgtcaaact cgttaacacc aatcaaagaa tcaatgcaag caagcctcac cctcggatga
      301 ccagtgatgc tttcatccaa agaataaact actttcaaag ccttttcata taatcctttt
      361 ttgttgcaca tgttcaaaca atcaacaact atgcgcacat caatttttgt catgcctgtc
      421 agcatcccaa gagccttgtc catatactcc acattgttct ccaatattcc aagacaccaa
      481 taactccata tatttggctt tagatcaatg gacttttgga agcattccaa agcgccatcc
      541 aaatcctcat tagccgcctt catgacacca agatgatata acgcatgcca gttaaaactc
      601 ttaggattct cctttgtaga tttctcaagc aggttcatcc actcaacctg ggttacatac
      661 tttgcaggtt cttcatttac cgggaactcc tcaaacacat tttcctcaag aagcctgagc
      721 cagatttcct gttccttccc aatattatca aacacaagac catgcaaaac ctttcctctt
      781 tcccttctct taagttccaa agctccccat ccgctgcctt catgcaccaa ttccacattg
      841 tcaatactaa aactctcatg tatctcatca agattctttc ttggaagcct cttttcaagt
      901 tcctctccca cacatttgta cacctttgtc caatcatcac catggacaat atccttatca
      961 gcttccaaat atccgtaagc ttcagtccag ttccagaccg aatttccttc cattggggtt
     1021 ctctgctgct gcatatgggc acatccagct tgaatctcac agtacgaact gcccttcaca
     1081 gacaaaaatt cctgccatcg tctgcctcct ctcgaatctc cccaattgaa aagctttctc
     1141 ccaagcagtc tgtccgtgga agtatgtacc agtcccttcc catcaccgtc cagagcgcat
     1201 atccattttc tctcttcttt tggaatatca aagaactggt caaacgcata aggtattctt
     1261 gccggatagc ttctgtcctg ttctccaagc atgggaaccg gatcctttcc aaagcttccg
     1321 tctgcatact gaacatacgc tttctcacaa ggcacaatga ttcttgtttt ctctgtctca
     1381 tccactgcta tattcgacca ccaatacatt ggaaccgttt cctcattgtc gtttataact
     1441 gtaatacgta cgaacagaaa tcttgacttt tcaggcagat acgcatctat ggaatatgca
     1501 agttttctaa tcctttccca ctcatacatt cttaaaaact tggtaccgtc cttcattgtc
     1561 cctacagcgg taaacaaagg ggaaacggta aaaggagtat gccccgtcat cccaatatta
     1621 aactccactc cacctgaaaa ccaggcattc ctgagcgcaa gatttgcagg ttgaaacacg
     1681 ggatttctat gaaccagatc tctcttgttt tccttgtcgt acaatgaata caatcttcca
     1741 cccagttctg gcaaaaacgt cgctctcaaa tattcgtttt caagaacaca agctttaaat
     1801 gcccttgtct tcatagccct gtcatagtca tcctgaatac aatagggaag tattgattta
     1861 attcttccgt aataaaaata ctctttttca tcctcgcgga tttcatcact gaatttgatt
     1921 tctcccaatc tctgccttaa tcctttgata tcgggtaatg gattttctcc atgcagtcca
     1981 gctgcaggta tttttagttc ttcaaaccta atttccgtca taaacaaacc tccttgatat
     2041 tcatcgattt gattatacta catattttcg ccctgtatag gtttattgtt gcaaatacaa
     2101 atatcaaatg ctacaattaa aacaaagcta tgaagaaaga ggcaacgaat atgagtaata
     2161 gattttatgg acttacctta ttggcattgc ttttggttat gacgttgata gttattacag
     2221 ggtgcaaagc acaagatgac acaaacccta ccccaactcc aacgaacaat acgatacata
     2281 atacacccgg cgagacggag aataccagta ctccatcaga aaccacgggt tccacttcca
     2341 cacccatgcc aacagaaact gaaacacaga ccgccactcc ggggccaaca gagacaaatg
     2401 caccagccac tgacacgccc actccaaaac caacaccaac acctacacca actcccacac
     2461 caaaattgga ggtaaccttc tcccatgaag gtggctttta cgataacaac ttcaagctgg
     2521 a
//
LOCUS       DAONVY010000353         2520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28000_length_2520_cov_2.475456, whole genome shotgun sequence.
ACCESSION   DAONVY010000353 DAONVY010000000
VERSION     DAONVY010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28000_length_2520_cov_2.475456"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /locus_tag="PLI11_08795"
     CDS             complement(<1..2006)
                     /locus_tag="PLI11_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPZ53002.1"
                     /translation="MNNAFKKLAPFIQEYIYKSKWKELRDIQIAACECIFNTQDNLLL
                     ASGTASGKTEAAFLPVISKIYKEKIDSVSVLYISPLKALINDQFERLIDLLKEANIPV
                     TKWHGDASSIGKERLRREPEGILQTTPESLEAMLVLRQHEIKYLFSDLRYIIIDEVHT
                     FMGSGRGIQLLCILERIQRSINRIPIRIGLSATLGDYTYAKKFLCAGTHRACQVPMIE
                     EKGRRLKLFVDFPKEEEFFLDLYKRTLNRQCIIFSNSKAEVEENIASLRRIAKEMGTM
                     DVYKVHHGNISKSMREEAETAMKMEEVKTVVGATLTLELGIDVGDLEMIVQTGAPLSV
                     SSFVQRLGRSGRKSGVGVMYFTFREKPGYGNMPIDKIDWELVKCIAVIELYLKERWIE
                     PVVAGQYPYEILLHQTLSHVASVHETRAAKLAEYMLTMSPFRNISQEDYRIMLLKMID
                     DGLLERTEEGFLHVGSLGEAIISNFDFFSVFETAIEYSVIYENREIGQVMVPYEEEET
                     FSLAGRAWQVVSIDKSSHVIHVKPHEEHAESRYKSDMRLIVPSKLVAKMFEVLSSDKA
                     YNYLGKRALERLFSSRKRFSALRIRDNVMQAGDKCLFLPRLGTKEIMYYSCALRMYGY
                     ENEIRYLSFVPIYISIPGSYANDVRDVVDKIEKHKPDKFDFHVDV"
     gene            complement(2003..>2520)
                     /locus_tag="PLI11_08800"
     CDS             complement(2003..>2520)
                     /locus_tag="PLI11_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HPZ53003.1"
                     /translation="NYEKILSMYNDTLQGKASHMGIIMALTPQSLEDPRKGVYSYEAL
                     RSRLESGWISTNNNGDMLAPVIKLAPLTQEETFVLLEKLVSIHSGLYGYESKVTDEDI
                     VAFLKIEFGRVGAQTFITPREIIRDFIELLNILYQNPDKTVQEVLGSSGFEFAISEDD
                     DVSDEFAEFTL"
BASE COUNT          679 a          616 c          432 g          793 t
ORIGIN      
        1 acatccacat gaaaatcaaa cttgtcaggt ttgtgttttt caatcttgtc aacaacatcc
       61 cttacgtcat tggcatagct tccaggtatt gaaatatata ttggcacaaa actcaaatag
      121 cgtatttcat tttcataccc atacattctt aatgcgcagg aatagtacat aatctccttt
      181 gtgccaagcc tgggcaaaaa caaacattta tctcctgcct gcatcacatt gtctctaatc
      241 cttaaagctg aaaatctttt ccgtgatgaa aaaagccttt ccaaagccct tttgccaaga
      301 taattatatg ctttatccga tgacagaacc tcaaacatct ttgcaaccag tttggatggg
      361 acaatcaacc tcatgtctga cttgtatctg ctttctgcat gctcctcatg cggttttaca
      421 tgaatgacat gagaactttt gtcaattgaa acaacctgcc acgctcttcc tgcaagggag
      481 aaggtttctt cctcttcgta aggaaccata acctgcccaa tttccctgtt ctcataaata
      541 acggagtact ctatggcagt ttcaaacact gaaaagaaat caaaatttga tatgattgct
      601 tctccaagag aacctacatg cagaaaacct tcctccgttc tttccaaaag cccgtcgtcg
      661 atcattttaa gcagcatgat tctgtagtct tcctgggata tgtttctgaa gggactcatt
      721 gtcagcatgt attctgcaag ttttgcagct cttgtctcat gtacggatgc aacatggctg
      781 agtgtctgat gcagaaggat ttcatatgga tactgtcctg ccacaacagg ttctatccat
      841 cgttctttta agtaaagctc aataactgca atacatttta caagctccca atctatcttg
      901 tctataggca tgttgccata gcctggtttc tccctaaaag taaaatacat gactcctaca
      961 ccgctttttc gaccgcttct tccaagtctt tgaacaaagg aggatacact aaggggagca
     1021 cctgtctgca caatcatctc caaatctccc acgtcaatac caagttccag agtcagagta
     1081 gctccaacca ccgtctttac ctcttccatc ttcatggccg tctccgcttc ctccctcatg
     1141 gattttgata tgttgccatg gtgaaccttg tatacatcca ttgtccccat ctctttggca
     1201 attctcctca gactagcaat gttttcttcc acttctgcct tgctgtttga gaatattatg
     1261 cattgcctgt tcaatgtcct tttgtacaaa tccaaaaaga actcctcttc ctttgggaaa
     1321 tccacaaaaa gcttaagcct tcttcccttt tcctcaatca tgggaacctg acacgctctg
     1381 tgtgttccag cacataaaaa ctttttggcg tatgtgtaat ctcccaaagt ggctgacaat
     1441 cctatgcgta tgggtattct gtttatgctc ctttgaattc tttcaaggat gcaaaggagc
     1501 tgaatacccc tgccacttcc catgaaggta tgaacctcat caatgataat gtatcttaaa
     1561 tcagaaaaca gatactttat ttcatgctgt ctcaatacaa gcattgcctc tagcgactcg
     1621 ggtgttgtct gcagtattcc ttcaggttct cttctcagtc tttctttgcc aattgacgaa
     1681 gcatctccgt gccacttggt aacagggata tttgcctcct tcagcaaatc aatcagtctt
     1741 tcaaactgat cattaatcag agcctttaac ggacttatat acaataccga aacggaatca
     1801 attttttcct tgtatatctt tgaaataacg ggcaaaaagg cagcctccgt ctttccggag
     1861 gccgtgcccg atgccaatag cagattgtct tgtgtgttga aaatacattc acaagcagca
     1921 atctgtatgt ctctcagttc cttccatttg cttttgtata tatactcctg tatgaaagga
     1981 gcaagctttt taaaggcatt attcataaag taaactccgc aaactcatct gaaacgtcat
     2041 catcttctga tatcgcaaac tcaaagccgc tagaacccag gacctcttgc accgtcttgt
     2101 ccgggttttg atagaggata ttcaaaagtt caataaaatc ccttattatc tctctggggg
     2161 ttataaaggt ttgtgcgccc actcttccaa actcaatctt gaggaaagcc acaatatcct
     2221 catccgttac ctttgattca tacccatata ggcctgaatg gatggaaacc aatttttcaa
     2281 gaagcacaaa ggtttcctcc tgagtaagcg gcgcaagctt tataaccggt gccagcatgt
     2341 cgccattatt gtttgtactt atccagcctg attcgagcct tgatcttagg gcctcataac
     2401 tgtagactcc ctttcttgga tcttccagag actggggtgt aagagccatg atgattccca
     2461 tgtgacttgc tttcccctgc aatgtatcat tgtacatgga aagaattttc tcataattgt
//
LOCUS       DAONVY010000354         2516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28057_length_2516_cov_2.986184, whole genome shotgun sequence.
ACCESSION   DAONVY010000354 DAONVY010000000
VERSION     DAONVY010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28057_length_2516_cov_2.986184"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PLI11_08805"
     CDS             complement(<1..1208)
                     /locus_tag="PLI11_08805"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPZ53004.1"
                     /translation="MLYKKNKSKELDSKLFKNPTAEYRGTPFWAWNCKLDKDNLGRQI
                     EYMKEMGLGGFHMHSRTGMANEYLGEEFMDLVKFCVKKAEKEEMLAWLYDEDRWPSGF
                     AGGLVTKEKKYRVKILTFNPEDKQNDAKTLEECLETGKPFFFASYDVVLNDKGELVSY
                     KRIGRDEEGNNKWYAFLEIAGDDPWFNNQAYVDTLDKESMKKFIDITYEAYKEAVGDK
                     FGESVPAIFTDEPQMYRRHYMRNALGKERTVLPWTHHLIDGFKKEYGYDILDYLPELI
                     WSLEGGKVSKVKYHYSDYTTELFARCFSDQCGNWCEENNLMLTGHMMEEPTLLSQSYA
                     IGETMRHYRSFQLPGIDMLCDATEYTTAKQAQSAAHQYGREGVLSELYGVTNWVFDFR
                     GHKFQGDWQAA"
     gene            complement(1245..>2516)
                     /locus_tag="PLI11_08810"
     CDS             complement(1245..>2516)
                     /locus_tag="PLI11_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53005.1"
                     /translation="KPTSTPPPTPYVDNTLTVDIGVFTFRNELGMKDGLYTIGKDFGM
                     LLEEKGEHEFNKYILTYTATDYLKGYIHYSLGRTSHVEEFFLEPGTNKEFSSLVDGFF
                     NNQKGTKIKKIVLNSIKGSSASFKLVDFKVEIVSVPVQVVYLENDYHKVGIDLLWGGA
                     INYIKDKTSKDGQLDNLLNRADTGRLVQQSYYGTMDPPYVKGEFMGNEWPYNPVQGGD
                     KGNNSSKIVDFKIYKEYIYIKCRPLDWGHINTPTFSYMENEYRLQGKTIVVNNRFVDF
                     SGYKAKPRHQELPAFYVISYLHTFVFYNGAKPWENDTLTYKRNLDFWGGNPDAYFNVR
                     QGNTETWCAWVDSNDYGIGLFTPNVEILLAGRFGYNGSKDPGNSATNYVAPLRTFEMK
                     SFEPFEYTYYITTGKIETIRQTFQTVFMAKS"
BASE COUNT          605 a          596 c          495 g          820 t
ORIGIN      
        1 gccgcctgcc agtctccctg gaacttgtgt cctctaaaat caaaaaccca gttcgttaca
       61 ccataaagtt cggaaagaac tccttctctt ccatattgat gtgcagcaga ttgcgcctgc
      121 tttgctgtag tatattcagt tgcatcgcag agcatatcaa tacctggcag ctggaaggac
      181 ctataatgtc tcattgtttc tcctatggca tagctttgag acagaagcgt tggctcttcc
      241 atcatgtggc ctgtcagcat gaggttgttt tcttcgcacc aattgccgca ctggtctgaa
      301 aaacatcttg caaacagttc ggtggtatag tcgctgtagt gatattttac ttttgaaacc
      361 ttgccacctt caagcgacca tatcagctcg ggcaggtagt caaggatgtc gtaaccgtac
      421 tcttttttga atccgtcaat gaggtggtgt gtccaaggca ggactgttct ttccttgccc
      481 agagcgtttc tcatgtaatg ccttctgtac atctgcggct cgtccgtaaa gattgcaggt
      541 acgctttcac cgaatttgtc gcctactgct tccttgtatg cctcataagt tatatctata
      601 aacttcttca tcgattcctt gtccagagtg tccacatatg cttgattgtt gaaccagggg
      661 tcatctccag ctatttcaag gaacgcatac catttgttgt ttccttcctc gtctcttcct
      721 atccttttgt atgacacaag ttcacccttg tcattaagga caacatcata gcttgcaaag
      781 aagaacggtt ttcctgtttc taggcactct tccagagttt ttgcgtcatt ttgcttgtct
      841 tcaggattga aggtcaatat cttgaccctg tacttctttt cctttgtcac aagacctcct
      901 gcaaatcccg atggccatct gtcctcgtcg taaagccatg caagcatttc ttctttttct
      961 gcttttttga cacagaactt taccaggtcc atgaattcct ctcccaggta ctcatttgcc
     1021 ataccggttc ttgaatgcat gtggaatccg ccaagaccca tttccttcat gtattcaatc
     1081 tgcctaccca aattgtcctt gtccagcttg cagttccaag cccaaaaggg ggttcccctg
     1141 tattcggcgg ttggattttt aaagagtttt gaatcaagtt cttttgactt gttctttttg
     1201 taaagcattg ttaccctcct atactttcat atgctgatat tatatcagga tttagccata
     1261 aatacagtct gaaatgtttg tcttatagtc tctatttttc ccgtggttat atagtaggtg
     1321 tattcaaacg gttcaaagga tttcatctcg aaggttctca agggggctac gtagttggta
     1381 gcagaatttc caggatcctt gcttccattg tagccaaacc ttccagcaag aaggatctcc
     1441 acattcggag taaaaagtcc tattccataa tcgttggaat ccacccatgc acaccaggtt
     1501 tccgtgtttc cctgccttac attaaagtac gcatctgggt tgcctcccca gaaatcaagg
     1561 ttgcgcttgt atgtcaatgt atcattctcc cagggttttg ctccgttata gaatacgaag
     1621 gtatggagat agctgattac ataaaacgca ggcagttcct gatgccttgg ttttgccttg
     1681 tatccggaaa aatcaacaaa gcgattgttg acaacaatcg tttttccttg aaggcggtac
     1741 tcgttttcca tgtagctgaa ggttggcgta tttatatgtc cccagtcaag aggcctgcac
     1801 tttatgtata tatactcttt gtatatttta aaatccacta tcttgcttga gttgttgccc
     1861 ttgtcgcctc cctggaccgg attgtaaggc cattcgttgc ccatgaattc acctttcaca
     1921 tagggaggat ccatggttcc ataatacgac tgctggacaa gtctgcctgt atcagcccgg
     1981 ttaagaagat tgtccagttg accatccttg ctggtcttgt ccttgatgta gttgatggct
     2041 ccaccccata acagatcaat ccctaccttg tggtagtcat tctcgagata gacaacctgc
     2101 acaggtacac ttacaatctc caccttgaaa tcaacaagtt tgaagctggc agaagatccc
     2161 tttattgagt tcaaaacaat cttctttatt tttgtacctt tctggttgtt gaaaaaccca
     2221 tccaccaaag acgaaaattc tttgtttgta ccaggttcca ggaagaattc ttccacatga
     2281 gaggttctgc ccaaagagta gtggatatat cctttcaaat aatctgtagc tgtgtaagtt
     2341 aatatgtact tgttaaactc atgctcccct ttttcctcaa gaagcattcc aaagtcttta
     2401 ccaatggtgt acaagccatc cttcatgccc agttcgtttc tgaaggtgaa tacaccaata
     2461 tccacagtca aggtattatc aacatatgga gttgggggcg gagttgaggt aggctt
//
LOCUS       DAONVY010000355         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28266_length_2500_cov_3.326789, whole genome shotgun sequence.
ACCESSION   DAONVY010000355 DAONVY010000000
VERSION     DAONVY010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28266_length_2500_cov_3.326789"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLI11_08815"
     CDS             complement(<1..520)
                     /locus_tag="PLI11_08815"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; lysine and threonine sensitive;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPZ53006.1"
                     /translation="MKVVKFGGSSLADANQIRKVCDIVLSDSKRRIVVVSAPGKRFDG
                     DVKVTDMLIELANKKLNNEDYTEALENVVDRYRQIAKGLGLGEDAANFVKHDLTDRLN
                     SKIDSNAKFMDFMKAAGEDNTAKLVAGYLNSIGEKAVYMNPKDAGMLLSNDYGNARVL
                     KQTYENLAKLRDM"
     gene            complement(538..1785)
                     /locus_tag="PLI11_08820"
     CDS             complement(538..1785)
                     /locus_tag="PLI11_08820"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPZ53007.1"
                     /translation="MVKIAIMGCGVVGSGVADILLEKSEELSRKFNKKVKLARILDIR
                     DMKGTPYEPYLTKNADEILNDPKINIMVMTIGGEDIAYTLTKKALEAGKHVVTSNKEI
                     VAAFGRELVETAYNNNVRFMYEASAGGGIPIIRPLNICLASNDIYEIQGILNGTTNYI
                     LTKMHRDKMTFEEALAKAQQKGYAEADPTADVDGHDAARKIAILSSVAYDKFVDYRDV
                     SCTGIRNICYEDLELSSANGFKIKLIARSINKDGKIEAVVEPLLLGEDHMLAKVDSVF
                     NGVLVKGSYTGDSMFYGKGAGKLPTASAILGDIIEILTGSVEKSLPGFVKEKAEMTTS
                     KQDSFRFFVRIWKNSISSEEMAKVVENSFGKVAIYCGESENNREKAALITGYLSREEL
                     DKKIEELTQSLNIKVENVIKVED"
     gene            complement(1795..2238)
                     /locus_tag="PLI11_08825"
     CDS             complement(1795..2238)
                     /locus_tag="PLI11_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPZ53008.1"
                     /translation="MAERYLLVSEDVCPAIFGKVVQAKKLLTLYKDKTQTEILNEVGI
                     SRSAFYKYKDKVFVYDRLPHSKVVSLYFVVEDFSGILHAIIGEIAKAKGNILTINQNV
                     PQNGIADVIISIDTECMIEDVDVVIRRTAGINGVRRAEILNREME"
BASE COUNT          647 a          638 c          381 g          834 t
ORIGIN      
        1 ccatatccct tagttttgca aggttttcat acgtttgttt aagtactctg gcatttccat
       61 aatcgttgct caaaagcatg cctgcatctt ttggattcat gtacacggcc ttctctccta
      121 tggaattcaa gtaccctgca acgagcttag ctgtattgtc ttctcctgca gccttcataa
      181 aatccatgaa ttttgcgttc gaatcaatct ttgaattcaa tctgtccgtc aaatcatgtt
      241 ttacaaaatt cgctgcatct tctccaagtc caagaccctt ggctatctgt ctatatctgt
      301 caacaacatt ttccaatgct tcagtataat cttcattatt caacttcttg tttgcaagtt
      361 ctatcagcat atcagtaact tttacatcac catcaaacct ttttcccggc gcggaaacaa
      421 ccactattct tcttttactg tccgacagta caatatcaca cacctttctt atctggtttg
      481 catcagccag cgaacttcct ccgaatttta caaccttcat aaatctactc ctttgctcta
      541 gtcttccacc ttgattacat tctcaacctt tatattcaaa ctctgggtta gttcctcaat
      601 ctttttgtcc agttcctccc tgctcagata gccagttatc agcgcggcct tttccctgtt
      661 gttctcagac tccccgcagt atattgccac cttgccgaat gaattttcaa ccactttcgc
      721 catctcctcg gatgatatgc tgttcttcca aatacgcaca aagaatctga agctatcctg
      781 ttttgatgtg gtcatctccg ccttttcctt aacaaaccca ggcagtgatt tctcaacgct
      841 tcctgtcaat atttcaatta tatctccaag aatcgcactg gcagttggca gtttgcccgc
      901 ccctttgcca tagaacatgg aatcgccggt atagctgcct tttaccagaa cgccattgaa
      961 aaccgaatca acctttgcca gcatgtgatc ctcaccaagt aaaagtggtt ccaccactgc
     1021 ttcaatcttt ccatccttgt ttatgcttct tgcaatcagc ttgattttaa aaccgtttgc
     1081 tgaactaagc tccaaatcct cataacaaat attccttata ccggtacagg aaacatccct
     1141 gtagtcaaca aacttgtcat aagcaacaga agacagaatg gcaatctttc ttgccgcatc
     1201 atgaccatcc acatccgctg tcggatccgc ttcagcatat cccttctgct gggcttttgc
     1261 aagagcttcc tcaaaggtca tcttgtctct gtgcattttg gtaaggatgt agtttgtcgt
     1321 gccgttcaga ataccctgta tctcatatat gtcattggac gcaagacata tgttcaaagg
     1381 acgaattatg gggattcctc ctcccgcact ggcctcatac atgaatctca cattgttatt
     1441 atatgcagtc tccacaagct cgcgtccgaa ggcggccaca atctccttgt tggaagtaac
     1501 cacatgtttt cctgcttcca gtgccttctt cgtcaaggta tatgcaatgt cttctccgcc
     1561 tattgtcatt accataatat ttatcttagg atcattcaat atctcatctg catttttcgt
     1621 caaataaggt tcgtaaggtg ttcctttcat gtccctgatg tccagtatcc tggccagctt
     1681 gaccttttta ttgaattttc ggcttaattc ttcgcttttt tcgagcaaaa tatccgccac
     1741 gccggagcct acgactccac aacccataat tgctatcttt accatatttc ctccttactc
     1801 catttccctg tttaatattt cagctcttct taccccgttt attccagcag tccttcttat
     1861 gacaacatcc acatcctcaa tcatgcattc cgtgtcaatc gatatgatta catctgcaat
     1921 accgttctga ggcacattct gatttatggt caatatgttg ccctttgctt ttgctatctc
     1981 gccaataatc gcatgaagga tgcctgaaaa atcctccaca acaaaataca aagaaacaac
     2041 cttgctgtgg ggtagtctgt catatacaaa aaccttgtcc ttgtatttgt aaaaagcact
     2101 tcttgatatg cctacctcgt tcaatatttc cgtttgtgtc ttgtctttat acagcgttag
     2161 cagtttcttt gcctgcacca ctttaccaaa tattgcagga caaacatctt cactaacaag
     2221 caaatatctt tcagccattt ttctaactcc tctgtccact gttaataaac atttgttcac
     2281 gtgtggtgga cacgaaggat ataatagcat atggttaagg aaaaatcaac aggcattttt
     2341 gtatttttat tgatatgatt attaagttat ggtaaaatcc atataaaaac atgggggaaa
     2401 tgcatggaga aactgacaat aaagctttat gacatattac tatgtttatc aaacgcattg
     2461 gaactattgt ctccaaaact atacaatcat catcagcaaa
//
LOCUS       DAONVY010000356         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28296_length_2498_cov_4.105608, whole genome shotgun sequence.
ACCESSION   DAONVY010000356 DAONVY010000000
VERSION     DAONVY010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28296_length_2498_cov_4.105608"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLI11_08830"
     CDS             complement(<1..399)
                     /locus_tag="PLI11_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53009.1"
                     /translation="MQINSFSVHACGNGKVLTYHHNADVFQFFGPFYSLPSFGEIRVT
                     DKYKKTFNDMAVEKEPNTNFYTYNVKGAVIKDFCFSEAPVFVRDIEGDDIVHTISVHD
                     YVMVKDVSFRYGKSDVKVFELKIISGTYIYF"
     gene            508..>2498
                     /locus_tag="PLI11_08835"
     CDS             508..>2498
                     /locus_tag="PLI11_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPZ53010.1"
                     /translation="MNIVRKIAKELDIKEYQVENTIKLIDEGNTIPFIARYRKEAHGS
                     LDDTTLRELEKKLNYLRNLESKKEEVKNLLEKQGVLDEKLIQEIEKADTLSEIEDIYR
                     PYRPKRRTRATIAKEKGLNPLAETIYQQIRIGMPLEEYIQKFIDDNKDTVTVESLEEA
                     INGVSDIIAEFVSDDAEIRKRVRGHFILNSQIVSKATDPETDSVYSNYYDYTESTIKI
                     ADHRILAVNRGEKEGFLKVTIQVNKENVLRLINNYVMKEDCQYRNFIERAIEDSYTRL
                     IEPSIEREIRNSLTERAVEGAIKNFSKNLKQLLMQRPLKGKVVMGIDPAYRTGCKFAV
                     CDDTGKVLDTGVIYPTPPHNKVEEAAKMLKAAIEKHNVDVISIGNGTASGETEMFVAS
                     LLKDIKRNIGYIVVNEAGASVYSASELAAEEFPDFDVALRSAVSIARRLQDPLAELVK
                     IDPKAIGVGQYQHDMPTNLLDSALKGVVEDSVNTVGVDLNTASFSILSYVSGINTSVA
                     REIVKYREENGKFKNRKELLKVPKLGAKTFEMCAGFLRIPESEDFLDNTAVHPESYGP
                     ARKLLDMCGIESTKDLKLVEERAKKIGVDKIARTIEVGVPTLSDIIEEIKKPGRDIRD
                     TLPSPIVRTEILTLENLKEGMILTGTVRNVIDFGAFVDIG"
BASE COUNT          899 a          494 c          500 g          605 t
ORIGIN      
        1 aaagtagatg tatgtacctg agatgatttt aagctcaaaa actttaacat cactctttcc
       61 atacctgaag gatacatcct ttaccattac ataatcatga accgatatgg tatggacaat
      121 atcatcgcct tcaatatccc ttacaaaaac aggagcctca ctaaaacaaa aatcctttat
      181 tacagcgccc tttacattgt atgtgtaaaa gttagtattt ggctcctttt caacagccat
      241 atcattaaat gtttttttgt atttgtctgt tactcgtatt tcgccaaagg aaggcagact
      301 gtaaaacgga ccaaagaatt gaaacacatc tgcattatga tgataggtaa gtactttacc
      361 attgccgcat gcatgtacgg aaaaactatt tatctgcata ttatcctcca actttatatt
      421 attttcaccc attatatcac aaaacctgat acagttttca tttttgtgct ataattatca
      481 tgaaaattta tatcaaagga gtttcacatg aacatcgtaa gaaaaatagc aaaagaactt
      541 gatattaaag agtatcaggt ggaaaacacc atcaagctta tcgatgaagg aaacaccatc
      601 cccttcatag caagatacag aaaagaagcc cacggctcat tagacgacac aaccttgaga
      661 gaactggaaa aaaaactaaa ttacctaaga aatcttgaaa gcaaaaaaga agaggttaaa
      721 aatctgcttg aaaaacaagg ggttttggat gaaaaactga ttcaggaaat tgaaaaggcg
      781 gacactttgt ctgaaattga agacatctac agaccctata gaccaaaaag aagaacaaga
      841 gccactatag caaaggaaaa aggtcttaat ccattggcag aaacaattta tcagcagata
      901 agaataggca tgcccttgga agaatatatt cagaaattca tagacgacaa caaggatacc
      961 gttacagtag agtctcttga agaagcaata aacggagtca gcgatataat tgctgaattt
     1021 gtcagcgatg atgctgaaat cagaaaaaga gtaagaggcc actttatcct aaacagccag
     1081 atagtatcaa aggctaccga cccggaaacg gattcagttt actcaaacta ctacgactat
     1141 acggaatcaa caataaaaat tgcagaccac agaatacttg cagtcaacag gggtgaaaag
     1201 gaaggctttt taaaagtcac aatccaggtt aacaaggaaa atgtacttag actaataaac
     1261 aactatgtaa tgaaagaaga ttgccagtac cgtaacttta tagaaagagc cattgaagac
     1321 tcctataccc gcctcataga gccctccatt gaaagagaga taagaaactc ccttacagaa
     1381 agggctgttg aaggcgctat aaaaaacttt tccaaaaacc tcaaacaact tcttatgcag
     1441 aggccgctga aaggtaaagt tgtaatggga atagaccctg catacaggac cggatgcaaa
     1501 tttgctgttt gtgatgacac aggcaaagtt ctggatacag gagtcatcta tcccactcca
     1561 ccccacaaca aggttgaaga agctgcaaaa atgctcaaag cagcaatcga aaaacataat
     1621 gtagatgtaa tatcaatagg aaacggtacc gcttccggtg aaacggagat gtttgttgca
     1681 tcactgttaa aggatattaa aagaaacatc ggatacatcg tagtaaacga agccggcgcc
     1741 agtgtttact cagcctccga acttgctgca gaagaattcc ccgatttcga tgttgcatta
     1801 agaagtgccg tatccattgc aagaagactg caggacccgc tggcagagct tgtaaagatt
     1861 gatccaaagg ctattggtgt aggacagtat cagcacgaca tgcccaccaa tcttttggac
     1921 tccgccctga agggtgtggt ggaagacagc gtcaatacag tcggagttga cttgaatacc
     1981 gcctccttct ccatcctctc ctatgtttcc ggcataaaca catcagttgc aagggagata
     2041 gtaaagtata gggaagaaaa cggcaagttt aaaaacagga aagaacttct caaggttccc
     2101 aaacttggtg ctaagacctt tgaaatgtgc gcaggtttct taagaattcc agaaagcgag
     2161 gattttctag acaacactgc agttcacccc gaatcctacg gacctgcaag gaaactactg
     2221 gatatgtgcg gaatagagtc cacaaaggac ttgaaactgg ttgaggaaag agcgaaaaaa
     2281 attggagttg ataaaatagc caggaccatt gaagtgggag tcccaaccct ttccgacata
     2341 attgaggaaa taaagaagcc aggaagagat ataagggata cattgccctc tccaattgtt
     2401 cgcaccgaaa ttctcaccct tgaaaacctg aaggaaggca tgattctgac cggaactgta
     2461 aggaatgtaa tagactttgg agcctttgta gacattgg
//
LOCUS       DAONVY010000357         2490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28402_length_2490_cov_2.495277, whole genome shotgun sequence.
ACCESSION   DAONVY010000357 DAONVY010000000
VERSION     DAONVY010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28402_length_2490_cov_2.495277"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /gene="hcp"
                     /locus_tag="PLI11_08840"
                     /gene_synonym="priS"
     CDS             complement(<1..1412)
                     /gene="hcp"
                     /locus_tag="PLI11_08840"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPZ53011.1"
                     /translation="MENAKMFCFQCQEALNCVGCTKAGVCGKKPEVSNMQDLLIYVTK
                     GLSYVATALRKEGKEVSKEVGRFVSTNLFVTITNANFDEEAIIDRIYKTLDLKKNLLG
                     QLKDLSKLPEAALWDKRNVEDMEKKARVVGILSIENEDIRSLKELITYGLKGLASYNK
                     HANVLGYSNEEIDAFAQETLAKLLDPAIGVEELVALALETGKYGVSTMALLNDANTST
                     YGKPEMTKVNIGTRNNPAILVSGHDLRDLEMLLEQTEGTGVDVYTHSEMLPAHYYPKL
                     KKFKHLAGNYGSAWWKQKEEFESFNGPILMTTNCLVPPKESYKDRVYTTGATGFPGCT
                     HIEPDENGHKDFSAIIEHAKRLSAPTQIEQGEIIGGFAYDQVMALADKVVEAVKSGAI
                     KKFIVMAGCDGRQKSRSYYTEFAQALPKDTVILTAGCAKYRYNKLDLGDIGGIPRVLD
                     AGQCNDSYSLALIALKLKEV"
     gene            complement(1500..2168)
                     /locus_tag="PLI11_08845"
     CDS             complement(1500..2168)
                     /locus_tag="PLI11_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPZ53012.1"
                     /translation="MIRIDSPLFTNLTDEEVLSCLRCSGARKRTYEKNEIIFDHDTKP
                     DKLYILLDGMVGLCMDSLSGDKSILTVLDKKGEIFGEVYMFIENNQYDYYALVLKKSV
                     VLEIPKVFFYQKCEKCCSYHSKLISNMLGILASKAYYLTKKNQLLSSTSLRQKLTKYI
                     LMNMDRQGFVKLNMSREDLANYLNVKRPSLSRELINMHRDNLIEVSGRTIKVVDIEKL
                     ADNL"
BASE COUNT          693 a          561 c          436 g          800 t
ORIGIN      
        1 aacacttctt taagtttgag agctatgagg gcaagggagt aagagtcgtt gcactgtcct
       61 gcatcaagaa ctcttggaat gccgccaata tcgcccaaat ccagcttgtt gtatctatat
      121 tttgcacaac ctgctgtaag gattactgta tcctttggaa gagcctgtgc aaattcagtg
      181 taatagcttc ttgatttctg tcttccatca caaccagcca tgacaatgaa ctttttgatt
      241 gcgccgctct ttacagcttc cacaaccttg tcagcaagag ccattacctg gtcatatgca
      301 aatccaccga taatttcacc ttgttctatc tgtgttggag cagaaagtct tttcgcatgc
      361 tctataattg cactgaagtc cttgtggcca ttctcatccg gctcaatatg tgtgcatcct
      421 gggaatcctg tagctccggt tgtatacacc ctgtccttgt agctttcctt tggtggaaca
      481 aggcagtttg tagtcataag gattggtcca ttaaaggact caaattcttc tttctgcttc
      541 caccatgcac ttccgtagtt gcctgccaga tgcttgaact tcttaagctt aggatagtag
      601 tgggcaggta gcatctctga gtgagtatat acgtctacac ctgttccttc ggtctgctca
      661 agcaacatct caagatccct aaggtcatga ccggacacaa gtatagctgg attgtttctt
      721 gttccaatgt tgaccttggt catttcgggt tttccgtagg tgcttgtatt agcatcattc
      781 aatagagcca tggttgatac gccatattta cccgtctcaa gagccagagc aaccagctct
      841 tcaaccccta ttgcagggtc aagtagtttg gccagcgtct cctgggcaaa tgcatctatt
      901 tcctcatttg agtaaccaag aacgtttgca tgcttgttgt aggatgcaag gcctttgagg
      961 ccgtatgtaa tgagttcttt aagactgcgt atgtcttcat tctctattga tagaatacct
     1021 acaactctag ctttcttttc catatcttcg acatttctct tgtcccacaa agcagcttcc
     1081 ggcagctttg acaaatcttt cagctgaccc agaagattct tctttaaatc aagggttttg
     1141 tatattctgt caataattgc ttcttcatca aaattcgcat tggttattgt aacaaaaagg
     1201 tttgttgata caaatcggcc aacttcttta gaaacctctt taccttcctt tctcaatgca
     1261 gttgcaacat aggacaatcc ttttgttaca taaatgagca agtcctgcat attggatact
     1321 tccggcttct ttccacatac acctgccttt gtacatccaa cacaatttaa agcttcctgg
     1381 cactgaaaac aaaacatttt tgcattctcc atcatattac ctcctaaatt aaatgtttat
     1441 gtaatttatc ctgacagaga aatagtacca tattgaaacc tgcatttctg taacatatgt
     1501 tacaagttgt cagctaattt ttcaatgtcc acaactttta tagttcttcc ggaaacttca
     1561 atcagattgt ccctatgcat gtttatgagt tccctggaca agctcggcct tttgacattc
     1621 aaataattgg caaggtcttc acggctcata ttcagcttca caaacccctg cctgtccatg
     1681 ttcatcagaa tgtatttcgt aagtttctgc ctcaagctcg tgctggacaa aagctgattt
     1741 ttcttggtca aataatacgc tttggaggca agaatgccca acatgtttga gatgagtttt
     1801 gaatgatatg agcagcactt ctcacatttc tggtaaaaaa acaccttggg aatctcaaga
     1861 acaacactct ttttaaggac caaggcgtag taatcgtact gattgttttc aataaacata
     1921 tatacctctc caaaaatctc acccttttta tccagcacgg tcaaaatgga tttgtctccc
     1981 gacaacgaat ccatgcaaag accaaccatg ccatcaagaa gtatatacag tttgtccggc
     2041 ttcgtatcat ggtcgaatat tatctcattt ttctcataag tcctcttgcg agcaccggag
     2101 catctcaagc aggagagaac ttcctcgtct gtcaaattcg taaacaatgg gctgtcaatc
     2161 cttatcatat tatcctcttt cactgataaa atatctggta taattataac ataagtttta
     2221 agttttagtt aagtttcccc aagccagttg tctgaagcga gcttaacata tataatatta
     2281 aatcatattg ttgtcaaata tattaggagg aaatgaatga gtaaaaaagt cattatacca
     2341 tttatagtat tggccgtagt agttgctgca atatccctcc atattatagg aaaaacatat
     2401 tacgaactgg ggtttgtttt aacaaaatcc acaccaagtg ctaccaatcc ccctgtaatg
     2461 aacagaacac caaagaataa aaatacacca
//
LOCUS       DAONVY010000358         2469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28716_length_2469_cov_2.425021, whole genome shotgun sequence.
ACCESSION   DAONVY010000358 DAONVY010000000
VERSION     DAONVY010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28716_length_2469_cov_2.425021"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLI11_08850"
     CDS             <1..380
                     /locus_tag="PLI11_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53013.1"
                     /translation="NARPVTPDGTALDYLSTSVTITVTYKVGGKATLYVNGEKQGATD
                     AVVAIGANDMYICIDHPQKKFSAVYEGLRFYNRELTAEEVAQNYEADLNPVNPATGVV
                     PTFFYMTVALGLAGNAFKRKKEE"
     gene            complement(443..1063)
                     /locus_tag="PLI11_08855"
     CDS             complement(443..1063)
                     /locus_tag="PLI11_08855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPZ53014.1"
                     /translation="MMKLIDLSQVIHNGMPVYPGDPNTKLSQIKYYEHDEYNNFKLNI
                     CVHSGTHIDAPMHLTESKQLVSEIPLESLIGEGCVLDVRYHDVITMKEEYKDLIKEGC
                     IVLLYTGFDKYYGTDKYYENHPCMDMDFCKFLVRMNIKMVGMDMPSPDKYPFEIHKML
                     LSNNIYIIENMTNLDKLLETDGFEVYAFPLRINADSSIARAVARVK"
     gene            complement(1203..2459)
                     /locus_tag="PLI11_08860"
     CDS             complement(1203..2459)
                     /locus_tag="PLI11_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPZ53015.1"
                     /translation="MKKYTNAVIYRHGDANEILVDNGVIKAIGTNLGPADEVIDLGGK
                     LVIAPYVDPHLHLDYVYTLSELGQAGAGSGTLFEAIEMWPKFKETLTVDSVKKLATKA
                     IRDEVSQGVQYIRTHVDVTDKNFTALRALLELREELKNIVEIQVVAFPQEGMYTFKGG
                     RDLVEEALKMGADVVGGIPHYEPAREFGEKSIYDTLELALKYDKLIDVHCDETDDPNS
                     RFVELLNAVVLMEGYGEKTTASHTCSFGSADNSYAFRMMDLFKKSKMNFIACPTENAY
                     LQGRQDTYPKRRGLTRVKEFMEAGINIAFAQDSINDPWYPMGNGNMMNILDNGIHLAQ
                     IMSPEEIEKDLDLITYNGARCMHIQDKYGLDASKDANFIVLDGDSSFDVIRNRAPVLA
                     SVRKGEYLFKRKPVEYEVALDLGIKY"
BASE COUNT          691 a          525 c          459 g          794 t
ORIGIN      
        1 ccaatgcaag acctgtaact cctgacggaa cggctcttga ctatctttct accagtgtaa
       61 ctattaccgt aacatacaag gttggaggca aggcaactct ttatgtcaat ggtgaaaagc
      121 aaggcgccac ggacgctgta gttgcaattg gtgccaatga tatgtatatt tgtatagacc
      181 atcctcaaaa gaagttcagc gctgtatatg aaggattacg tttttacaac agagagctga
      241 cggctgagga ggttgcccaa aactatgagg cagaccttaa tcctgtcaac cctgcaacag
      301 gtgttgttcc gacattcttc tacatgacgg tagcactggg acttgcagga aacgccttca
      361 agagaaagaa ggaagaataa ttaattttgt ctgcaagata aagggctgta gcaaatgctg
      421 cagtcctttt acataatgta tgtcatttta ctctcgctac tgctcttgct attgagctgt
      481 cagcatttat tcttaaaggg aaagcataga cttcaaatcc gtctgtttcc agcagtttgt
      541 caagatttgt catgttctca ataatataaa tattgttgga tagcagcatt ttatgaattt
      601 caaagggata tttgtctgga gagggcatgt ccattccaac cattttaata ttcatcctca
      661 caagaaactt gcaaaaatcc atatccatac atggatgatt ttcataatac ttgtctgttc
      721 cgtaatattt atcaaatcct gtgtacaaaa ggacaatgca gccttccttt attaaatctt
      781 tatactcttc tttcatagtg attacatcat gatatcttac atctagcaca catccctctc
      841 caattaacga ttcaagtggt atttcggaaa caagttgctt gctttcagta agatgcatgg
      901 gcgcatctat atgtgtacct gaatgcacac atatattcag tttgaagttg ttgtattcat
      961 catgttcata gtatttgatc tgacttagtt tagtattcgg atcgccagga tagacaggca
     1021 tgccgttatg tattacttgt gataaatcaa tcagtttcat cagctaatct cctttgaaaa
     1081 catacatgtt tctgaaaaaa tactttatct taaagcaata aaaatgtaaa gggtctgtgt
     1141 gatatgtatt atttaaaggt agtcgtcgca ttagttgaaa tataaactaa tgcgacttta
     1201 tatcaatatt ttatccctaa atcaagtgca acttcgtatt caacaggctt tctcttgaat
     1261 agatattcgc cttttctgac tgaagcaaga actggggcac gatttctaat aacatcaaac
     1321 gaactgtccc cgtcaaggac aatgaagttt gcatctttgc tagcatcaag gccgtacttg
     1381 tcctggatgt gcatgcagcg tgcaccattg tatgttatca aatccagatc cttttcaatc
     1441 tcttcagggg acatgatttg tgcaagatgt attccattgt caagaatgtt catcatattt
     1501 ccgttaccca taggatacca tgggtcgttt atggagtctt gcgcaaaagc aatattaatg
     1561 ccagcttcca taaactcttt tactcgggtc aatccgcggc gttttggata agtgtcctgg
     1621 cgtccttgca gatatgcgtt ttccgttggg catgctataa agttcatttt gctctttttg
     1681 aacaaatcca tcattctaaa tgcatatgaa ttgtcggcag agccaaacga gcatgtgtgg
     1741 cttgctgttg tcttttcgcc atatccttcc ataagaacaa ccgcattcag aagttcaaca
     1801 aaacgtgaat tagggtcatc ggtctcatcg cagtgtacat caatgagttt gtcatatttc
     1861 agagcaagtt ccaacgtgtc gtatattgat ttctcgccaa actccctggc cggctcgtag
     1921 tgtggtattc ctcctacaac atcagcgccc attttaagag cttcctcgac caaatcacga
     1981 ccgcctttga acgtatacat accttcctgt gggaatgcaa caacttgaat ctcaacaata
     2041 ttttttaact cttctcttag ttcgagaaga gctcttaaag ctgtgaagtt tttgtctgta
     2101 acgtccacat gtgttcgaat gtactgaact ccctgggaaa cctcatctct gattgctttt
     2161 gttgcaagtt tctttacact atcaactgtc aatgtctctt tgaactttgg ccacatttca
     2221 atagcttcga aaagggttcc tgaaccagcg ccagcctgtc caagttccga caaagtgtat
     2281 acgtaatcca ggtgcaagtg aggatcaaca taaggagcta taaccagttt tcctcccaga
     2341 tcaattacct cgtctgcagg cccaagattc gttccaatcg cttttataac tccgttatct
     2401 accagtattt catttgcgtc tccatgacga tatattactg cattagtata tttcttcata
     2461 atgtcctcc
//
LOCUS       DAONVY010000359         2468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28724_length_2468_cov_4.592209, whole genome shotgun sequence.
ACCESSION   DAONVY010000359 DAONVY010000000
VERSION     DAONVY010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28724_length_2468_cov_4.592209"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2402)
                     /locus_tag="PLI11_08865"
     CDS             complement(<1..2402)
                     /locus_tag="PLI11_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPZ53016.1"
                     /translation="MINLIIEQDGDWHVGIYSAPQSDLSKVKESSDVDSYSVMCNIGY
                     AYLDGGLNEYKPYLFLVSFTEDTFKSFPLKVIEGRLPENESEIVIPSHIYTNGGVKFA
                     VGDRITLDIGHRMTHDGYRLTQSNPYIKLEDLEEQEYLDVFTAKTYTVVGICERPGFE
                     RYVSPGYTVITYNDNPSLTDNEFDLFFKVKKPSKVYEVVGDLKLEGDFGYHGELLRFM
                     GVSSIGNLNAVLYSIGSILLVLIMTCSISLIYNSFSISVSERTRQYGLLSGAGATKKQ
                     LKASVFYEALYISVIGIPLGILAGILGIGVTLNLLEDTFKSIASANSTVRFTLSVSVA
                     SIAVAAVAGIITILLSAYIPARRCARLSAMDAIRQVADIKMKPRNVKTWRITRKVFGI
                     EGDLALKNIKRNKKRYRSTVISLFVSIVLFISASSLSSYMIDSVTTVYQNLNYDLSYR
                     TGLNDKFDEKTQAVYERITRLGSIKKSSVINNMYARLTLPKSKVDDDYYNLIKSHGFL
                     SDGDNLDIFISLYYLDDVTFKEFLKEIGENQALYFDEENPRCLVVDRQQYFDYTQERM
                     IDQNILKQEVVKEIDVTIDNEQGYEPVKLAIGKYVSDIPIGIRHYSDYNSITVMIPQS
                     IKDSVFAEYKDMWDTNLTMYFTSDNPFKAAEEIAEILLEEGLSTSKLSNHAEILANTR
                     NIVTIINVFAYGFIILISLITVANVFNTISTNMELRTREFAMLRSVGMTSKGFRKMVS
                     FESVFYGLKALIYGLPVAVGITYLIYKSMLQGVDVRFYIPATSMCITVIMVFVVVFAT
                     MT"
BASE COUNT          694 a          546 c          477 g          751 t
ORIGIN      
        1 tatgtcattg tggcaaacac aaccacaaac accattatca ccgttatgca catgctggtt
       61 gcaggaatgt agaaccttac atccactccc tgaagcatgc ttttatatat caggtatgtt
      121 attccaacag ctacaggcaa gccgtatatg agggccttga gtccataaaa gacactctca
      181 aagctgacca tttttctaaa acctttggat gtcatgccta ccgaacggag catggcaaac
      241 tctctcgtac gcagctccat attggttgat atggtgttga atacattggc aacggtaatt
      301 agggatatga ggattataaa tccataggca aacacattta ttatcgtcac tatgtttctc
      361 gtattagcca gtatttcagc atggttggac aatttggatg tgcttagccc ttcttccagc
      421 agtatttccg caatctcctc ggccgccttg aaaggattgt ctgacgtaaa atacatggtc
      481 aggttagtat cccacatatc cttatattca gcaaacacgc tgtcttttat ggattgagga
      541 atcattaccg ttatggagtt gtagtcgctg taatgtctta tacctatagg gatatcagaa
      601 acatattttc ctatagccag tttgacaggc tcataccctt gctcattgtc tattgtcaca
      661 tcaatttctt tcacaacctc ttgctttagg atgttttggt ctatcatcct ctcctgggta
      721 tagtcaaaat attgctgacg atccacgaca agacagcgag ggttttcttc atcgaaatac
      781 agggcctggt tttcaccaat ttccttcagg aattccttga aggtaacatc atccaaataa
      841 tacaggctta taaatatgtc cagattgtcc ccatcagaca gaaatccatg actttttatg
      901 agattatagt aatcgtcatc aaccttgctc tttggcaggg ttaatctagc atacatgttg
      961 ttaatcacac tggacttttt gatgctgcca agtcttgtta ttctttcata cacggcttgt
     1021 gttttctcat caaacttgtc atttaaccct gttctgtagg acaagtcgta attcagattc
     1081 tggtatactg ttgtcacgct gtcaatcatg tagcttgaca aagacgaggc tgatatgaaa
     1141 agaactatgc tgacaaagag cgatatgaca gtggaacggt atcttttttt gtttctcttg
     1201 atgttcttta gggcaagatc cccttcaata ccgaaaactt tccttgttat tctccaggtc
     1261 tttacattcc tcggcttcat ctttatatcc gccacctgac gaatagcgtc cattgcagat
     1321 aaccttgcac agcgtcttgc aggaatgtag gcggataaaa gaatggtgat aatacctgca
     1381 acggctgcta cagcaatcga tgccactgaa acgctcagag taaaacgtac agtgctgttt
     1441 gcactggcaa tggacttgaa ggtatcttcc agaagattta gagtaactcc aattccaaga
     1501 atacctgcca gaatgcctag gggtatgccg ataacgctta tgtataaagc ttcataaaat
     1561 acagatgcct tgagctgttt tttagtagca ccagcaccgg acagaagtcc atactgtctc
     1621 gttctttcac taactgatat tgagaaagag ttgtatatga gagagatgga gcatgtcatg
     1681 ataagcacaa gcaagattga gccaatactg taaagaactg cattcaggtt gcctatggag
     1741 ctcactccca tgaaccggag cagctcgcca tgatagccaa aatctccttc aagctttaaa
     1801 tctccaacca cttcatatac ttttgatggt tttttgacct tgaaaaacaa atcaaattca
     1861 ttgtctgtca gactcgggtt gtcattgtac gttatgacgg tgtatcccgg agaaacatac
     1921 ctctcaaagc caggacgttc acagataccg acaacagtat aagtctttgc agtaaagaca
     1981 tccaggtatt cttgttcctc caaatcctcc agttttatat acggattgga ctgagtaagt
     2041 ctgtatccgt catgtgtcat tctgtgacct atgtccaggg taatcctgtc ccctacagca
     2101 aacttgacac ccccattggt gtatatatga ctgggaatta caatttcact ttcgttttcg
     2161 ggaagacgac cttcaataac ttttaacgga aacgatttaa aggtgtcctc ggtgaaggat
     2221 acaagaaaca gatagggctt gtattcattc agcccaccgt ccagatatgc atatccaata
     2281 ttgcacatca cagagtagct gtccacatcg ctgctttctt taactttgct cagatcgctt
     2341 tgtggtgcac tatatattcc aacatgccag tcgccatcct gttcaattat cagattaatc
     2401 atatatgact gcaagctgga tataaatgtg gtaaccgcag ttatcattga aagggacagt
     2461 atgacacc
//
LOCUS       DAONVY010000360         2463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28812_length_2463_cov_3.117110, whole genome shotgun sequence.
ACCESSION   DAONVY010000360 DAONVY010000000
VERSION     DAONVY010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28812_length_2463_cov_3.117110"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..939
                     /locus_tag="PLI11_08870"
     CDS             40..939
                     /locus_tag="PLI11_08870"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HPZ53017.1"
                     /translation="MELFLKSFMHEQNFPEDARECFLNLHNRIDKDKVIHDLVFLTVD
                     RFFENDISFNDLDEFFKTCAFQAGTHEYTLAMLFFMYCARVLEKKYLQRGIDRKIYVD
                     TMNDLKCKLMECREVYGINGTFVAHWYPQFFTLERFALGRLQYEKIPFPKEDYSKNGY
                     TVRKGSIVYNCHIPSSGSLTEEMCFESYKKAYEFYKDELGDNPMVIVCHSWLLYPENV
                     DFFPKHSNILKFMSHFDIISYDTHDSFSEAWRVFGKNGLLPPSKWKTETSLQRAFKKH
                     VLSNGRTGNGYGVLIFDGENIVN"
     misc_binding    992..1236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1315..>2463
                     /locus_tag="PLI11_08875"
     CDS             1315..>2463
                     /locus_tag="PLI11_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPZ53018.1"
                     /translation="MIITDFLERNAKLYPDKISLVEINPENEPDRAITWREFNLIETT
                     SSEKYRREMTWKEFDVKANRFANLLLTRGIQKGDKVAILLMNCIDWLPIYFGILKTGA
                     LAVPLNYRYTAEEIQYCLELADVKALIFGPEFIGRLEQVFDHLKNVKTMFYVGDNTPT
                     FADSFNKYVTYCSSTAPAVELLEDDDAAIYFSSGTTGFPKAILHSHRALVSACVTEQS
                     HHMQTHNDVFLCIPPLYHTGAKMHWFGSLMTGSKAVLLKGVKPEWILQAVSDEKVTIV
                     WLLVPWAQDILDAIERGDVCLDEYRLAQWRLMHIGAQPVPPSLIKRWLKYFPNHQYDT
                     NYGLSESIGPGCVHLGVENIHKVGAIGKAGHGWETAIVDEHGRPVAQGE"
BASE COUNT          708 a          463 c          572 g          720 t
ORIGIN      
        1 actgggagca atttgttgta tttaaaagga gtatcattta tggagttgtt tttaaaaagt
       61 tttatgcatg agcagaattt tccagaggat gccagagagt gttttctcaa cctgcacaac
      121 cgcattgata aagacaaggt tatacatgat ttggtgtttt taacagtcga tagatttttt
      181 gagaatgaca tttcttttaa tgacctcgac gagttcttta agacatgcgc atttcaggca
      241 ggtactcatg agtatactct tgccatgctg ttctttatgt actgtgcaag agtccttgaa
      301 aagaagtatt tacaaagagg cattgataga aaaatatatg ttgatacaat gaatgactta
      361 aagtgcaagc tgatggaatg tagggaggtt tacggtatta acggtacttt tgttgcccat
      421 tggtatccac agttttttac gcttgagagg tttgctcttg gtaggctcca gtatgaaaag
      481 attcctttcc ccaaggaaga ttactcaaag aacggataca ccgtgcgcaa gggaagcatt
      541 gtctacaact gccacattcc ttcatctggc tctttgactg aggagatgtg ttttgagtct
      601 tacaaaaagg catatgagtt ttacaaagac gagcttggag acaatcccat ggtaattgtt
      661 tgtcactcat ggcttctcta ccctgaaaat gtagactttt tcccaaagca ttctaatatt
      721 ctaaagttta tgagccattt tgacattatt tcctatgata ctcacgattc cttcagcgaa
      781 gcatggaggg tttttggcaa gaacggtctg cttccgccta gtaagtggaa aactgaaaca
      841 tccttgcaaa gagctttcaa aaaacatgta ctgagcaacg gcaggaccgg caacggatat
      901 ggtgtcttga tatttgatgg tgaaaatatt gttaattgaa cggtcttgaa ttaacttgaa
      961 tctattggta taattgttga aaatataata gggaaatgcg atgactggaa acaagtaggt
     1021 atgttctttc ctttaagaga gctgtcggtt ggtgcaagtc agtaggaaaa catagccgaa
     1081 tacattccaa gagcagtgta ctgaacagct ttatggctta gtaggctaca acgcgagcac
     1141 gcgttacagt gctagagcaa tttatgctcg ctgaggttgg ttttcgtgag aaagtcaacg
     1201 aattgaggtg gtaccacggt taattccgtc ctctgactat tgcatttaat tattgcaaag
     1261 ccggaggacg ttttttagtt cctccgctag atataatcag aaaggtggat tctaatgata
     1321 attactgatt tccttgaaag aaacgccaaa ctttaccctg acaagattag tcttgtggag
     1381 attaatcccg aaaacgaacc cgatcgtgcg attacatggc gggaatttaa tttaattgaa
     1441 accacttcca gcgaaaagta cagaagagaa atgacatgga aggagtttga cgtgaaggca
     1501 aaccgttttg caaatctcct tcttacaaga ggtattcaga aaggcgataa agttgcaatt
     1561 ctgcttatga attgtattga ctggctccct atttacttcg gaattcttaa aacaggagct
     1621 ttggccgttc ctttaaacta cagatatact gcagaggaaa tccaatactg tctggaactg
     1681 gctgatgtaa aagcgcttat ctttggccca gagtttatcg gtcgcctgga gcaggttttt
     1741 gaccatctca agaatgttaa gaccatgttt tacgtagggg acaatacccc cacatttgct
     1801 gacagcttta acaaatatgt tacttactgc tcatctactg ctcctgcagt tgagctgctc
     1861 gaagatgacg atgctgccat atacttttca tccggaacca ccggtttccc aaaagcaatc
     1921 cttcacagcc acagggctct ggtttctgca tgcgttacag agcagagtca ccacatgcaa
     1981 actcacaatg atgtgttttt gtgcattcct cccttgtatc ataccggtgc aaagatgcat
     2041 tggtttggca gccttatgac aggaagcaag gcagtacttc ttaagggtgt aaaaccggaa
     2101 tggatactgc aagccgtttc cgacgagaag gtaactatcg tttggcttct agtgccttgg
     2161 gcacaggata ttctggacgc cattgaaaga ggggatgtct gtctagatga gtacagactc
     2221 gcccagtgga ggttgatgca tataggggca cagcctgttc ctccaagtct gattaaacgc
     2281 tggctgaagt attttccaaa ccatcaatat gatacgaatt atggtctgag cgaatctatc
     2341 ggacccggtt gcgtgcattt aggtgtcgag aacatacata aggtgggagc tataggaaag
     2401 gcaggacatg gctgggaaac tgctattgtg gatgagcatg gcaggccagt tgcacaggga
     2461 gaa
//
LOCUS       DAONVY010000361         2457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28912_length_2457_cov_4.030808, whole genome shotgun sequence.
ACCESSION   DAONVY010000361 DAONVY010000000
VERSION     DAONVY010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28912_length_2457_cov_4.030808"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..741
                     /locus_tag="PLI11_08880"
     CDS             196..741
                     /locus_tag="PLI11_08880"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HPZ53019.1"
                     /translation="MLTYFNYFLTYSFCGFLLETIFAFIVKGRLESRKCYLISALCPV
                     YGLGAIAINESTRKISGSKTKTFFVGMLAATVVELLTDLFYKHVLGMRFWDYSKRPFN
                     LWGSVCLKYSLFWGLLSLLLVYVVHPYVEKNARKIKKGTSYAFMLLFIADSIISAFLL
                     RKFKTKHAVNYAWIRKAFRRS"
     gene            complement(728..1585)
                     /locus_tag="PLI11_08885"
     CDS             complement(728..1585)
                     /locus_tag="PLI11_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPZ53020.1"
                     /translation="MYDAIVVGKGPAGLQAAIYIARAGLSVIVIGENDSALLKAIHVD
                     NYFGFEDTISGRELLEQGERQAKRFGAKILTAHVLNISYADNGFDVKTNVDTYNAKTI
                     LIATGKPLPPAIIPGIKKFEGKGVSYCTTCDGFFFRGKAVGILGNKDYAISEAEEMEK
                     FTPNITIYTNGRELEVEKSKYRVITKKIKALQGENVLEKVVFEDDTEEDLQGLFIALD
                     RPAGAEFARKLGIEIENDRIKTDEDFQTNIPGIFAAGDCVSDFKQISVAVGQATLAAR
                     GMIKYAKNA"
     gene            1661..1969
                     /locus_tag="PLI11_08890"
     CDS             1661..1969
                     /locus_tag="PLI11_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane trafficking protein"
                     /protein_id="HPZ53021.1"
                     /translation="MANLFDRIDGKIMEAKIDQGIDMLKNKSPDEISKKISKMDKDEI
                     MQKLDELDEKRIKELNIDLDKIKKKITPEDIEKAKKIAGKDGEVIINRLLELLKQGGK
                     "
     gene            1969..2334
                     /locus_tag="PLI11_08895"
     CDS             1969..2334
                     /locus_tag="PLI11_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53022.1"
                     /translation="MDKETEEKLKQLGSMFGITEIPDNLGEVLESFLSGQSDNANEES
                     QKEEKNDTDLGIDIDADTIRLITTLKKSLDRNKSDDKARLLKALTPFLNEKKQQKVNN
                     ALKLITFAEIAKNKDLLGL"
BASE COUNT          761 a          458 c          492 g          746 t
ORIGIN      
        1 tatgaaaaac ccgaatcatt cggtcttgag caatcaacct tcggttccga tcactcaaaa
       61 gtttcctatg aaagaacagt cgctgccttg tccgaagcgg ttgaagcata caatgaatgg
      121 ataggaagca gttctcctgt tgctgcatga attaatattt gtcttgtgag ccaaaatatt
      181 gcaggggtga atattttgct cacatatttc aactattttc taacttacag tttttgtggt
      241 ttcttacttg agacaatatt tgcattcatt gtaaagggaa ggcttgaatc aagaaaatgc
      301 tacttgataa gtgccttgtg tcctgtttac gggcttgggg ctattgccat caatgaaagc
      361 acgaggaaaa tatcaggcag caagacaaaa accttttttg ttggcatgct tgccgcaacg
      421 gtagtggaac tactaactga cttgttctac aaacatgtat tggggatgcg tttttgggat
      481 tactcaaaaa gacccttcaa tttatggggc agtgtgtgcc tcaaatattc cttgttttgg
      541 ggacttttgt ctttgcttct ggtttacgtg gtgcatccat atgttgagaa gaatgctaga
      601 aaaattaaaa aaggcacttc ttatgcgttt atgcttctat ttattgcaga tagtataatc
      661 agtgcctttt tgttaagaaa attcaaaaca aagcacgctg tgaattatgc ctggattaga
      721 aaagcattca ggcgttctta gcatacttta tcatgcctct tgctgccaaa gttgcctgtc
      781 caaccgctac ggatatctgt ttgaagtctg atacgcaatc tcctgcagca aaaatacctg
      841 ggatgttggt ctgaaaatcc tcgtctgttt ttattctgtc gttttcaatt tcaataccaa
      901 gttttcttgc aaattcagct cctgcaggtc tgtcaagggc gataaacaaa ccctgcaaat
      961 cttcttccgt atcgtcttca aaaactactt tctcaagtac gttttcgccc tgcagggctt
     1021 tgattttctt tgttattacc ctgtacttgc ttttctccac ttccagctct ctgccatttg
     1081 tataaatcgt aatatttggt gtaaatttct ccatttcttc cgcttcgctt atggcatagt
     1141 ctttgttgcc cagaattcca accgctttcc ctctgaaaaa gaaaccatca caagttgtac
     1201 agtagctgac cccttttcct tcgaatttct taataccagg aattattgcc ggaggcagtg
     1261 gctttcctgt agctataagt attgtttttg cattatatgt gtcgacattg gtttttacat
     1321 caaagccgtt atctgcatag gatatgttaa gaacgtgcgc agtaagtatc tttgcgccga
     1381 atctctttgc ctggcgctca ccttgttcca aaagctcacg tccgctgatt gtgtcctcaa
     1441 acccaaaata gttgtcaaca tgtattgcct ttaatagagc ggaatcgttt tcacctatta
     1501 ctattacgga caatcctgct cttgcgatgt atatggcagc ctgcaaacct gcgggacctt
     1561 ttcctaccac tattgcgtca tacataaaaa ttcctccttt tagaactatt tttaattttc
     1621 ctacatatta tatactaaaa catatcagga ggtaaaattt atggcaaatt tatttgacag
     1681 gatcgatgga aagattatgg aggcgaaaat agatcagggg atagatatgc tgaaaaacaa
     1741 aagtcctgac gaaatatcca agaaaatctc aaaaatggat aaggatgaaa taatgcagaa
     1801 gctggatgag ttggatgaaa aaaggataaa ggaattgaac attgatctgg acaaaattaa
     1861 aaagaagatt acaccggaag acatagaaaa agccaaaaag attgctggca aagacggcga
     1921 ggtaattatt aatcgtctgc ttgaattgct taaacaaggt ggtaaataat ggacaaggaa
     1981 accgaagaaa agctgaagca actggggagt atgtttggaa taactgaaat accggacaat
     2041 ttgggagaag ttctggaatc ctttttgtca ggacaaagcg ataatgcaaa tgaagaaagt
     2101 caaaaagagg agaaaaacga tacggacctg gggattgata ttgacgcgga tactataagg
     2161 ttaattacta ctttgaaaaa aagcctggac agaaataaga gtgatgataa agccaggctt
     2221 ttgaaagcct tgactccttt tctgaacgaa aaaaaacaac agaaggtgaa taatgcgcta
     2281 aagctgatta catttgctga aattgcaaaa aacaaggatt tattaggtct ttaactgggg
     2341 ttgataaaat tgccagaaaa catcataaat gacaattatg aaaacaatcc agaggttgta
     2401 atggtatctc ctctggataa cccctatgat tccaaaccag tacatgcttc caaaaag
//
LOCUS       DAONVY010000362         2457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_28915_length_2457_cov_3.452123, whole genome shotgun sequence.
ACCESSION   DAONVY010000362 DAONVY010000000
VERSION     DAONVY010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28915_length_2457_cov_3.452123"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLI11_08900"
     CDS             complement(<1..315)
                     /locus_tag="PLI11_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPZ53023.1"
                     /translation="MSKFCVKCGTKMDKEEKNCPKCGMKSIKLPDPAQLKKRKKKIIT
                     ITSVAVVLIALVTILIVYFANSSLVLSDIFTGLRGKTIITVNGYKVPENEFRFFCSLV
                     LED"
     gene            complement(391..2067)
                     /locus_tag="PLI11_08905"
     CDS             complement(391..2067)
                     /locus_tag="PLI11_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53024.1"
                     /translation="MVKFLKRLLVFCLVVLVIYVILIIPKVDNFITAKIDSLYTTNPE
                     PEYIINTEDMYEENESPPLTFIREERLWLLYQDRFVDITTEGLRLDSINYDINKSFEL
                     KKNCRVVSNGKHIMYILEINGESHLFRLDIENREVVEIAEKVDSFVITKRGNILYATG
                     YLSSNYVYVYKDGQSKQIAKDAKVYYLRDHDCIIARQSDGALIRYTEFQDDTVILDTD
                     VKELYNPFGKKHNYIDMSNSLLLYYKKATGDYVNNNGEVKALEKSFHETVPETIYPCV
                     KDDRYFYYSKKEKTLYLMDEGDIYGIMRDVYKIFEYDKETERFVLATSKAVYLATIGE
                     TNRVDAVKMYNLKGIYKRNESMIRRHLDIVTNDFKSFYSVQISGSSFIFNFINADSWM
                     NKISIVKYKLHVSTLQGDEFESIKELDVPQSRKLEDNLIIYDEKTYIYIPRNKKMEGS
                     SVSFITKNITTYQNSIENEPFDTVQTYNGLDYIYFVTETTKNNVKNRLYVYENNTSIM
                     LHRGSFKTVKALSSIYLISQNEDGETYDISIINGLTVDRVVRNVEFANES"
     gene            complement(2060..>2457)
                     /locus_tag="PLI11_08910"
     CDS             complement(2060..>2457)
                     /locus_tag="PLI11_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53025.1"
                     /translation="TGMSKYNRLTGYGAVSNDEKGKYTFHLSLTNKELANISRSSFAS
                     DADLVYHITGDEDNGIYCLVNINGTGNYRLNYFASMKDFVSQMKTLNSLRGIVGKVCS
                     HISTIIYIYIGFATWFILKIKYYRRNGNG"
BASE COUNT          631 a          493 c          465 g          868 t
ORIGIN      
        1 gtcttcaagc acaagggagc agaagaatct gaactcgttt tccggcacct tgtacccgtt
       61 aaccgttata atggtctttc ctctcagacc tgtaaatata tcggagagaa ccagcgaact
      121 gttggcaaaa tacacaatca gtatggtaac taaagctatg agcacgacag ccacgctggt
      181 tattgttata attttctttt ttcttttctt tagttgggcg ggatcaggaa gttttattga
      241 cttcatgccg cactttgggc aatttttttc ctccttgtcc atcttcgtcc cgcattttac
      301 acaaaactta gacatgttgc tcctttcaaa tataaccata ttgtattata tatattatca
      361 tataccacga cttaagtcat aaaaaaatta ttaactctca ttggcaaatt ctacattcct
      421 gacaaccctg tcaacagtaa ggccgttgat tatcgaaatg tcataggtct ctccgtcctc
      481 gttctggcta attagataga tggaggagag ggccttgact gttttgaagc tgcctctatg
      541 caacataatg gatgtattgt tctcatatac atacagcctg ttctttacat tgttcttcgt
      601 agtttcagta acgaagtaaa tataatcaag accgttgtat gtttgaaccg tgtcaaaggg
      661 ctcgttttca atggagtttt ggtaagttgt tatgttcttt gttataaaag acaccgagct
      721 tccttccatc ttcttgtttc tgggaatata tatgtatgtt ttttcatcat atattatcag
      781 gttgtcttca agcttgcggc tttgtggcac atccagttcc tttatacttt caaactcatc
      841 cccctgcaat gtgcttacat gtagtttgta cttcacgatg gaaatcttgt tcatccagct
      901 gtcggcattg ataaagttga aaataaagga ggaacctgat atctgaacgg agtagaagct
      961 tttgaaatcg ttggtcacta tgtccagatg ccttcttatc atgctttcat ttctcttgta
     1021 tatgcctttc aagttgtaca tttttacggc atccacccgg tttgtttctc caattgtggc
     1081 aaggtaaacg gccttcgatg tggcaagaac aaatctctcc gtttctttgt cgtattcaaa
     1141 tatcttgtat acatccctca ttattccgta gatatcgcct tcgtccataa gataaagggt
     1201 tttctccttt ttggagtagt agaagtatct gtcatccttg acgcaaggat atattgtttc
     1261 aggtacggtt tcatgaaagc tcttttcaag agccttaact tcgccattat tgttcacgta
     1321 gtctccggta gcttttttgt agtacaggag gagtgagttc gacatgtcta tgtagttgtg
     1381 ctttttacca aagggattgt acaactcttt tacgtcggta tcgagtatta ccgtatcgtc
     1441 ctgaaattca gtatagcgga ttaaggcacc atctgactgt ctggcaatga tgcagtcgtg
     1501 atctcttaga taatatactt ttgcatcctt tgcaatctgc tttgattggc cgtctttgta
     1561 aacatacaca tagtttgaac ttaagtatcc ggtagcgtag agaatgtttc ctcttttggt
     1621 tatgacgaag ctgtctactt tttctgcaat ttcaaccact tccctgtttt caatatccag
     1681 cctgaataaa tgggattctc catttatctc aagaatgtac atgatgtgtt tgccgtttga
     1741 cacaaccctg cagttctttt tcagttcaaa tgacttgttt atatcgtagt ttatggagtc
     1801 gagtctaagg ccttcggtgg ttatgtctac gaatctatcc tgataaagaa gccagagtct
     1861 ttcttccctt atgaatgtga ggggaggact ttcgttttct tcgtacatgt cctcggtgtt
     1921 gattatgtac tctggttcag gatttgttgt gtacaagctg tcaatctttg cagtaatgaa
     1981 gttgtccacc ttgggtatga tgagaattac gtagatgacc agcacgacca ggcagaaaac
     2041 cagtagcctt ttcaaaaatt taaccattac cattcctcct gtaatatttt atctttagta
     2101 tgaaccatgt tgcaaaacct atatatatgt atatgatcgt ggaaatgtga ctgcatactt
     2161 ttccgacaat cccccttagg gaattcaagg ttttcatttg agaaacaaaa tctttcattg
     2221 atgcaaaata attgagtctg tagtttcctg ttccgttaat attaacaaga caatatatgc
     2281 cattatcctc atctcctgta atatgataga ccaggtctgc atccgatgca aagcttgatc
     2341 gggatatgtt ggcaagctct ttgttcgtaa ggcttaaatg gaatgtgtat ttgccttttt
     2401 catcatttga aaccgctccg taaccagtca atctgttata cttcgacatg cctgtca
//
LOCUS       DAONVY010000363         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29026_length_2450_cov_3.812109, whole genome shotgun sequence.
ACCESSION   DAONVY010000363 DAONVY010000000
VERSION     DAONVY010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29026_length_2450_cov_3.812109"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLI11_08915"
     CDS             complement(<1..332)
                     /locus_tag="PLI11_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPZ53026.1"
                     /translation="MRIDFLTLFPNVIKACFDESIIGRAIDKGLLDFRYYQIRNYSKD
                     KHRKVDDYPYGGGPGLVMSYQPIYDAFMDIKDNSDDRPWCIYMSPQGQVLTQKKAMEL
                     SRRSHLVIL"
     gene            complement(322..711)
                     /gene="rimM"
                     /locus_tag="PLI11_08920"
     CDS             complement(322..711)
                     /gene="rimM"
                     /locus_tag="PLI11_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPZ53027.1"
                     /translation="MKEYEYQSSRILDNVILLALKDVDTRDKALNLKGAYISVSREDA
                     IKLPEGRYFIADLIGCTVYEHTGEILGKVKDVFETGSNDVYDVVNDEGKSILVPALEK
                     VVKSIDVENRKIIVELLPGLKEIYYEN"
     gene            complement(708..845)
                     /locus_tag="PLI11_08925"
     CDS             complement(708..845)
                     /locus_tag="PLI11_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53028.1"
                     /translation="MVERFVIGKIVNAHGIRGELKVLPETEDPTDLRNLKKFGSRRIV
                     P"
     gene            complement(939..1166)
                     /locus_tag="PLI11_08930"
     CDS             complement(939..1166)
                     /locus_tag="PLI11_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003368285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPZ53029.1"
                     /translation="MKELLVYIARQLVDNTDAVSVNQIETEKAITLQLSVAEEDMGKV
                     IGKDGRIAKAIRTVIKAASRNEDKPVYVDIV"
     gene            complement(1186..1434)
                     /gene="rpsP"
                     /locus_tag="PLI11_08935"
     CDS             complement(1186..1434)
                     /gene="rpsP"
                     /locus_tag="PLI11_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPZ53030.1"
                     /translation="MVKIRLKRIGAKKAPYYRVVVADSRNARDGRFIEEIGTYNPLVD
                     PAEIKIDVERADYWIKNGAQMTDTVKALYKRVTEKTAE"
     gene            complement(1467..>2450)
                     /locus_tag="PLI11_08940"
     CDS             complement(1467..>2450)
                     /locus_tag="PLI11_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPZ53031.1"
                     /translation="TASGKLALHLKKQGKNPLLVACDVYRPAAVKQLEVLGGQIGVNV
                     YQEGTDENPVKIAKNAVKQALRGLNDVVIIDTAGRLQIDEELMVELEKIKEATNPSEI
                     LLVVDAMTGQDAANVAQTFNDRLDVTGIILSKLDSDTRGGAALSVKAVTGKPVKFSSV
                     GEKMNEFEVFYPDRMADRILGMGDVLTLIDKAMEAIDLEKAEELEKKMRKQQFTLDDF
                     LDQMQQVKKMGGISSILGLLPGVAGKINEEDIDEKKLVKTEAIIHSMTLKERKDPSIL
                     NASRRKRIAQGSGTTVQDVNRVIKDFENIKRMMKMMQGAGKKGFGKMRLPF"
BASE COUNT          622 a          638 c          397 g          793 t
ORIGIN      
        1 agaatcacaa gatgagatct tctactcagt tccatggcct ttttctgtgt cagaacctgg
       61 ccctgaggtg acatgtatat gcaccagggt ctgtcatccg agttatcctt tatatccata
      121 aaagcatcgt atatgggctg ataggacatg acaagaccgg gacctccacc gtaaggataa
      181 tcatccactt tcctgtgctt gtccttgctg tagttcctta tctgatagta tctgaaatca
      241 agcagccctt tatcaatagc cctgccaatt atgctttcat caaagcatgc ttttataaca
      301 ttggggaaca aagttaaaaa gtcaattctc atagtaaatt tcctttaatc ccggtaatag
      361 ctctacaatg atttttctat tctcaacatc aatggacttg accacttttt ccagagctgg
      421 tacaagtatt gatttgcctt catcgtttac tacatcgtaa acatcattgc tgccagtctc
      481 aaaaacatcc ttcaccttac caagtatctc tccggtgtgc tcatacaccg tacacccaat
      541 aaggtccgct ataaagtatc ttccttcagg cagcttaatg gcatcctccc tggaaacgga
      601 aatataggct cctttaagat tcagagcctt gtcacgtgta tccacgtctt tcaatgcaag
      661 cagaataaca ttatccaaaa ttctactgct ttgatattca tattctttca aggcactatc
      721 cttctcgatc caaacttttt taagtttctt aaatctgttg gatcctcggt ttcaggtaac
      781 accttgagtt cgccccgtat cccatgagca ttgacaattt taccaataac aaaacgctct
      841 accatttaca acctctttac acatatattc atgcaaatag caaaagaggg agcggcaaac
      901 ccttccccca ttaatcagct cataaccaaa tgatagtctc aaactatgtc cacatacacc
      961 ggcttgtctt cattcctgga tgctgcttta ataacagtcc taatggcttt agctatgcgg
     1021 ccatccttgc ctatgacttt tcccatatcc tcttcagcaa cactaagttg cagagtaatc
     1081 gccttctcag tttcaatctg attgacagaa accgcatccg tattgtcaac caattgacgg
     1141 gcgatataaa cgagcagctc tttcataaag caatcctcca atctcttact cagctgtttt
     1201 ctcagttact ctcttgtaca gagccttgac agtgtcagtc atctgtgcac catttttaat
     1261 ccaatagtct gccctttcga catctatctt gatctcagca ggatccacaa gcggattgta
     1321 ggttcctatt tcctcgataa atctgccgtc tctggcattc cttgagtctg ctacaacaac
     1381 tctataatat ggagcctttt tagcacctat tcttttaagt ctgattttta ccacaccaag
     1441 cacctccttt gtattaattg gttatgttaa aaaggtaatc gcattttgcc aaaacctttc
     1501 ttacctgcac cttgcatcat cttcatcatt cttttgatgt tctcaaaatc ctttatcacc
     1561 cggttaacat cctgcactgt agtgccgcta ccctgggcta ttctttttct acggcttgca
     1621 tttagtattg aaggatcttt tctttctttc aaagtcattg aatgaataat agcttctgtc
     1681 tttacaagct tcttttcatc aatgtcttcc tcgtttatct tgcctgcaac tccaggcagc
     1741 aacccaagta tactgcttat accacccatt ttcttgacct gctgcatctg atcaagaaaa
     1801 tcatccagtg tgaactgctg cttgcgcatc ttcttttcca gctcttcggc cttctcaagg
     1861 tcgatggctt ccatggcctt gtcaattaac gtaagcacgt cacccatacc aaggattctg
     1921 tccgccattc tgtccggata gaagacttca aattcattca tcttctcgcc tacggacgag
     1981 aacttcacag gtttgccggt tacagctttt actgacaagg cagcaccgcc acgggtatca
     2041 ctgtcaagtt tggacagaat tatgccggtg acatccagtc tatcattgaa tgtctgtgcc
     2101 acattcgcag catcctgacc agtcattgca tctactacca gcaaaatttc ggagggattt
     2161 gttgcttcct ttattttttc caattccacc attaattcct catctatctg cagtcttccc
     2221 gcagtgtcga tgattactac atcattaagc cctctgagcg cctgtttgac cgcatttttt
     2281 gcaatcttta caggattttc atcagtacct tcctgataaa cattaacccc aatttgtcca
     2341 cccagtactt ccagttgctt tactgcagca ggtctgtaca cgtcacaggc aaccaaaaga
     2401 gggtttttgc cctgtttttt cagatggagt gcaagctttc ccgaggctgt
//
LOCUS       DAONVY010000364         2443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29139_length_2443_cov_3.351340, whole genome shotgun sequence.
ACCESSION   DAONVY010000364 DAONVY010000000
VERSION     DAONVY010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29139_length_2443_cov_3.351340"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="PLI11_08945"
     CDS             complement(<1..1592)
                     /locus_tag="PLI11_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HPZ53032.1"
                     /translation="MKKILLTVIAFILLGLVIVCSNDIGTVNQWVASEMTFESEQDYA
                     SPFYDVDFDVIFTHKKTGFELKIPGFWDGGKVFKVRFALTQVGEWSFVTVCSDKDNTG
                     LHNKSGRIMCVEYNGELEIYKRGFVKTDPNLRYFVYDDGTPFFYLGDTHWVIGEEDIS
                     TPESKFYKIIDHRVKQRFTVYQSQPLGVPYLLSDGVSEDDLKGFRALDVRFKYIADSG
                     LVHANSQLFHVRELMKYKMPMYTDEYLDRLCRYWVARYSAYPVLWTTAQESDNDFYFE
                     RGDSIFDAANNRWIKVAQCIHKYDPYKHPLTAHMEHTSFCTASGSSFRDVEGHTWYAA
                     QFSFNYDMPMPLDIVKDYWENGQGKVVINYEGKYDHLWTKTFGSRAQGWIAFLNGMYG
                     YGYGAQGIWDVWFNNEDTYDGRDIITPEDKMVTWQEALKFPSGDQVTIMRSFLEEYEW
                     WKLTPRFDDKGYMESRSSFRNEYKELITRKNIHYSLATIGNDLYVLYLFNNTVQSATL
                     KGLTNTIYTAKWFNPRTGEYIN"
     gene            complement(1589..>2443)
                     /locus_tag="PLI11_08950"
     CDS             complement(1589..>2443)
                     /locus_tag="PLI11_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53033.1"
                     /translation="RKYNRVLKDYEDIEKIKDMEITQHKETCDYIYQQAKDIFGGIAP
                     VMQGHGIQFHLGVWDFLTTYIGVEDAYYEIMDRPDFVHACLKRITEATIAGIKKANDL
                     EVHNDIANTCHCSYVYTDELLPDFGAGKGPVSYNSWAFGMAQLFSTASPKVTEEFELP
                     YISKMAEYFGMIYYGCCDRLDDRLDIVKKIPNVRKVSCSPWSDRKAFAEKIGDNLVMS
                     FKPNPAYVAGGSFDEDIVRKDLRYTFDLAKANNVNLEIILKDISTVCFDPSSLARWAK
                     IAMEVVQS"
BASE COUNT          641 a          562 c          463 g          777 t
ORIGIN      
        1 tcattaatgt attcaccggt tcttgggttg aaccattttg cagtataaat tgtatttgta
       61 agacctttta aagtagcaga ctgcactgtg ttattaaaca agtacagtac atacaagtcg
      121 ttgcctattg tagccaggct gtagtgtatg tttttgcgtg ttatcagctc tttatattcg
      181 ttcctaaagg aacttctgct ttccatatat cctttatcat caaatctagg agttagtttc
      241 caccattcat attcttctaa gaaggaccgc ataatagtca cctggtctcc agatggaaac
      301 ttcaaagctt cctgccaagt aaccatttta tcctcagggg taataatatc cctgccatcg
      361 tatgtgtcct cattgttgaa ccagacatcc catattcctt gagctccata cccataacca
      421 tacattccat tcaggaaggc aatccagccc tgggctcttg aaccaaaagt ctttgtccaa
      481 agatggtcgt actttccttc gtagtttata actacttttc cttgtccgtt ttcccagtaa
      541 tccttgacta tgtccaaagg cataggcatg tcgtaattga aagaaaattg agcggcgtac
      601 caggtgtgcc cttcaacatc tctgaatgac gacccgcttg ctgtgcaaaa gctggtatgt
      661 tccatatgtg cggtaagagg atgtttgtat ggatcgtatt tatgaatgca ctgtgcaact
      721 tttatccatc ggttgttggc tgcatcgaaa attgagtctc ctctttcaaa gtagaagtca
      781 ttgtcgcttt cctgggcagt cgtccataaa accgggtagg cactatacct ggcaacccag
      841 tatctgcata atctgtccag atactcatca gtgtacatcg gcattttata tttcatgagt
      901 tctcgcacat gaaacagctg tgaattggca tgcacaagac cgctatcggc aatatacttg
      961 aatcttacat ccagagcccg aaaacccttg agatcatctt ctgaaacccc atcggagagc
     1021 aagtatggca ctccaagagg ttgtgactgg tatacggtga atctttgctt gaccctgtgg
     1081 tctatgattt tataaaactt ggactcgggt gtggaaatat cctcttcgcc aatcacccaa
     1141 tgtgtgtctc ccagatagaa gaagggtgta ccatcatcat atacgaaata cctgaggtta
     1201 ggatctgtct ttacaaaccc ccttttatat atctcaagct ctccattata ttccacacac
     1261 attattctcc ccgacttgtt atgcagcccg gtattatctt tgtcagagca tacggtaaca
     1321 aagctccatt cgccaacctg tgttaatgca aaccttactt taaatacttt gcccccatcc
     1381 cagaaaccag ggatcttaag ttcgaatcca gttttcttgt gagtgaatat tacgtcgaag
     1441 tctacatcgt aaaatggact tgcatagtct tgttcgcttt caaaagtcat ttcggatgca
     1501 acccactggt tgactgtgcc aatgtcatta ctgcaaacaa ttacaaggcc taaaagtata
     1561 aaagctatta cagttaacag aatctttttc atgattgtac aacctccatg gcaattttgg
     1621 cccagcgtgc caggctgctt gggtcaaagc atactgttga tatgtctttc aatattattt
     1681 cgaggtttac gttgtttgct tttgccaggt caaacgtgta tcggaggtct ttccttacaa
     1741 tatcctcatc aaacgaacca ccggctacat atgccggatt tggtttaaat gacatgacaa
     1801 gattgtctcc aatcttctca gcaaatgctt ttctatcgct ccatggactg caggatactt
     1861 ttcgtacatt tggtatcttt ttcacaatat caagcctgtc atcaaggcgg tcacagcagc
     1921 cgtaatatat catgccgaaa tattctgcca ttttcgagat atatggcagc tcaaactcct
     1981 cggtaacctt gggagatgct gtggaaaaga gctgtgccat gccaaatgcc catgaattgt
     2041 acgatacagg acctttgcct gcaccaaagt caggcaacag ttcatcggta tatacgtatg
     2101 agcagtggca tgtgtttgcg atatcgttat ggacctccaa atcgtttgcc ttcttgatac
     2161 ctgcgattgt tgcttctgta attctcttca agcaggcatg aacaaaatct gggcgatcca
     2221 tgatttcata atatgcatcc tcaacaccaa tgtatgttgt taaaaagtcc cagactccca
     2281 aatggaactg tattccgtgt ccttgcatga ccggtgcaat gccgccaaat atgtcctttg
     2341 cctgttggta aatataatcg catgtttctt tgtgttgtgt gatttccata tcctttatct
     2401 tttcgatatc ctcatagtct ttcaataccc ggttgtattt tct
//
LOCUS       DAONVY010000365         2443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29145_length_2443_cov_2.064908, whole genome shotgun sequence.
ACCESSION   DAONVY010000365 DAONVY010000000
VERSION     DAONVY010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29145_length_2443_cov_2.064908"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1170)
                     /locus_tag="PLI11_08955"
     CDS             complement(316..1170)
                     /locus_tag="PLI11_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ53034.1"
                     /translation="MQKMTGFQQEGLLLHHIMQDVPIARQFMNHHHQNEYEICVVQKG
                     KGSFIVEGIEHPIKDRTVFFFKPLENHMASIEFPNMYDRFTVIFSKNSIDGDSLPMSL
                     LLATLNNKPSGKFNYFTDEDINFDLITACLRNMMDPDFYNLKVTITAHLYPVLYELRK
                     AYTKLDPNAAKIECYPELFSQVLDYIKVHYAENITLDFLTNHFYISKSTLHRMFKKYT
                     NTTFYQYLTRVRMLEAKELVKETDLSFSQISQQCGFSDYSAFYRLYKSFFMMSPQADK
                     KGSLFNNN"
     gene            1549..2352
                     /locus_tag="PLI11_08960"
     CDS             1549..2352
                     /locus_tag="PLI11_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53035.1"
                     /translation="MRKISLLIVALIFLFTLSVSSNDDITKNLNTNLTGLRVINGEWK
                     SVENGVFGDNDGVGNCFMMSDVKRGAGIAFRYSAEISVDNVAGGIVFGVENPDSPEDI
                     WYCMNIDAGNMDAPLVRLFKVDGTELVWSNNVVKHDYEKGQSYTLTIEVFEDGSIDFY
                     VDDEYMASQADTGYEGGYLGIKTCVSSVTFNNINYEIIESTPTPEKTQTPEPSSTPVP
                     EETEEKPKTTDESKPDTDEEDNTMWVVASIALGVVIVVLVVLIIIKKRK"
BASE COUNT          749 a          378 c          543 g          773 t
ORIGIN      
        1 gttgtacgag cattccttgt gtgcaaagat gatatgggag agactgtata tttttacagc
       61 gatgtgcagg aaaaatcgct aactgatgtt tacaacagta tagttgaaag tgaaggtgct
      121 catcaattgg atccggatgt tttaagatgg tggaatgagg tgtaagtatg aaaatacatt
      181 atgatgaacc tgaaatggag attgtatatt ttgaagcaga agaagagatt aactccagtg
      241 gtgattacaa tggagttgac tttcttgact tgatataatt acctatatat ttacgacaac
      301 tgagagcgca agctctcagt tgttgttaaa caggcttcct tttttgtcag cttgcggtga
      361 catcatgaag aaactcttgt ataatctata gaaagcggag tagtcggaaa aaccgcattg
      421 ttgactgatt tgggaaaagc tcaagtctgt ctcctttacc aattccttgg cttcgagcat
      481 tctgaccctg gtcaggtatt gataaaatgt ggtgtttgta tattttttaa acatcctatg
      541 caatgtgctt ttacttatgt aaaaatgatt tgtcagaaag tccaatgtga tgttctcagc
      601 atagtggact ttgatataat cgagaacttg agaaaagagt tcaggatagc actctatttt
      661 tgcagcgttc ggatcaagct ttgtataagc tttacgcaat tcgtaaagaa cagggtacaa
      721 atgagcagtt attgttactt ttaggttgta aaagtcaggg tccatcatgt ttcgaagaca
      781 agctgtaatc aaatcaaaat taatatcttc atcggtgaaa tagttaaatt tgcccgatgg
      841 cttgttgttg agagtagcta gcagcaagga catgggcagg ctgtctccat caattgaatt
      901 tttcgagaaa attactgtaa acctatcata catgtttgga aattcaatac tggccatgtg
      961 attctcaaga ggcttaaaga aaaagacggt tctgtccttt atgggatgtt ctatcccctc
     1021 aactataaaa ctgccttttc ctttctgcac aacacatatt tcatattcat tttggtgatg
     1081 gtgattcatg aactgtctgg caattggcac gtcctgcata atgtgatgta ataataggcc
     1141 ttcctgctgg aagccggtca ttttctgcat attatcctcc gtctgcattc aaaagtagtg
     1201 tctttttgtg catagtctaa cacagcaagg tgttaatttc aaggatgcag aatatacttt
     1261 gcacatgatg cgtattaatg ttgtattggt aataggtatc tccagtcacg tggagtgtca
     1321 aatatcaggc tcaataaaaa ttggttgatt taaaactcag gttaaacagc ctgagtttgc
     1381 tttataatgc caaatagtct gtttttctca ccatattcaa aaatatttca ctacataaat
     1441 attaaggaat tgttaaacta tacatatacg ataaggcaaa atggagatca tttatgagat
     1501 ggatagtact catggtgtat taaagaacag aatttggagg aatgaagcat gagaaaaatc
     1561 agtttgttaa ttgtcgctct aatcttttta tttactttat ctgtgtcgtc caatgacgat
     1621 ataaccaaga atctgaatac aaatctaact ggccttagag tgataaacgg agagtggaaa
     1681 agtgtagaga atggtgtctt tggagataat gacggtgttg gcaactgctt tatgatgtcc
     1741 gacgtaaaaa ggggagcggg gatcgctttt agatattctg cagagatttc tgtagataat
     1801 gtagcaggcg gcattgtctt tggtgtagag aatccggaca gtcccgaaga catatggtac
     1861 tgcatgaata ttgatgcagg caacatggat gcgcctttgg ttaggttgtt caaggttgat
     1921 ggtactgagc tagtttggtc caacaatgta gttaaacacg actatgaaaa gggacaatct
     1981 tatacactta caattgaagt gtttgaagat ggttctattg atttttatgt ggacgatgaa
     2041 tacatggcgt cacaggctga tactggctat gaaggtggat atctgggtat taaaacctgc
     2101 gtgtcttctg ttacctttaa caatataaac tatgagataa tcgagtcaac accaacgccg
     2161 gagaagactc aaactcctga accatcatca actcctgtgc ctgaggaaac tgaagaaaaa
     2221 cccaagacaa cagatgagag caagcctgat acagatgaag aagacaatac aatgtgggta
     2281 gtagcttcaa ttgctctggg tgtcgtaatt gttgttttgg tagtgttgat aattattaag
     2341 aagaggaagt gagtttgtat gaaaaaaaca tttgctcttg tattgttttt gctgattttt
     2401 ggtatgctga tgtcctatgc acaggaaata gaatggtatc aga
//
LOCUS       DAONVY010000366         2433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29296_length_2433_cov_2.731707, whole genome shotgun sequence.
ACCESSION   DAONVY010000366 DAONVY010000000
VERSION     DAONVY010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29296_length_2433_cov_2.731707"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1450
                     /locus_tag="PLI11_08965"
     CDS             83..1450
                     /locus_tag="PLI11_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ53036.1"
                     /translation="MKAYSYIKTFTCVFCILMLVILMGCNRNDIHIGDLPPEYQANVT
                     GNITVSYPMHIAMDDESRLSMQGFARAFMDKYKGASVTIDFADYGNKEVRIASGDIGD
                     VYWMGEEETYTNAITHNSLMALDAYIEILGIDISNVYSGIYNLGIADARLYMVPRDHN
                     HIVFFYNVDALNEVGLATPPDDWTWEMFKDYCRKLTVEEADGTYSRIGGTWHISWGPN
                     LIPFLEGWGGKWFDDVNKKVLFVSDEKVLKGFSELVGVIDDGLVRPDGVSGNFMSKYA
                     KLNVEDTVFRAGVFPGLYQAGLEYQSKGVNWNIANWPRFPVHRVGTGATGFGVYNRTK
                     RPDTAAAFCLFLFTDEGQRAYHEQVGGSVPLTKNLAMEDFWRIPWPKDKINYDAFISY
                     PEADTVGKFQCRLPDSVASIILSRINNVFEAHLSGRTDYKDSLGEIEKLATEKWETLF
                     EGERT"
     gene            complement(1476..>2433)
                     /locus_tag="PLI11_08970"
     CDS             complement(1476..>2433)
                     /locus_tag="PLI11_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPZ53037.1"
                     /translation="DRAVVLCDLNVFEELGVKVPDSNWTYEDFVETVEALTQKKNSKS
                     YTGVYLPYHQAFVWMQFVRGFGGEWFDEDNLDLVLTQGKSFEGLSSMFMLLNRGYAQA
                     NGFDAKNSLNAKCGMSFAFASQPDRNGMYFLSEEELMRNPANYAEELAKDNNLLILPL
                     PKFPEGDVGASNTDFIHGFAMVDSSKKKNLAAKLIGFSQSVEGQKILNKYYGGIPSNV
                     DAQKEDFWKVGVFASQANAENALMGIEHDRRDDFFEALHDSKDLFEENIRLRAMFSRV
                     LFRDLERLNNKGTKDRVGDAVVDALKQLERQSSITIKLAERR"
BASE COUNT          734 a          523 c          507 g          669 t
ORIGIN      
        1 ttagcagcca gctaattttg aaatataata taatcaacgt aaaaggtcag tcgagataca
       61 tacttggaaa ggagttttaa ttatgaaagc ttatagttac ataaaaacat tcacctgtgt
      121 tttctgcatt ctcatgcttg tcatactaat gggatgcaac agaaacgata ttcatatagg
      181 agatctgcct cctgaatatc aggcgaatgt gacgggcaat atcacagtat catatcccat
      241 gcatatagcc atggatgatg aaagcagact gtcgatgcag ggcttcgcta gagcattcat
      301 ggacaaatac aaaggtgcga gcgtgaccat tgacttcgca gactacggaa acaaggaagt
      361 gcgcatcgca tcaggagata ttggagatgt gtattggatg ggagaagagg aaacgtatac
      421 gaatgccata acccataact ccctgatggc acttgacgca tacattgaga ttcttggaat
      481 agacatatcc aatgtctatt caggcatata caacctagga attgcagatg ccagattgta
      541 catggttcca agggatcaca atcacatagt ctttttttac aacgttgacg cattgaacga
      601 ggttgggctg gcaactccac ccgatgactg gacatgggaa atgttcaagg attactgcag
      661 aaaactgaca gtagaagagg ctgatggaac ctattcaaga atcggcggta cctggcatat
      721 atcctgggga cccaacctaa tacctttcct tgaaggctgg ggcggaaaat ggtttgacga
      781 tgttaacaag aaagtcttgt ttgtaagtga tgagaaagta ttgaaaggtt tttccgagct
      841 tgttggagtt attgacgacg gtcttgtacg tcctgatgga gtaagtggca acttcatgtc
      901 caaatatgca aaactgaatg tagaagacac cgtattcagg gcaggagttt tccctggtct
      961 ttatcaggca ggattggaat atcagtccaa gggtgtaaac tggaatatag caaactggcc
     1021 acgattccct gttcatagag taggaacggg agccacaggt tttggagtat acaacagaac
     1081 aaaaagaccc gatacggccg ctgctttctg tctgtttctt tttacagacg aaggacaaag
     1141 ggcatatcat gaacaggtag gcggttctgt tccccttact aaaaacttgg ctatggaaga
     1201 tttctggaga ataccatggc caaaagacaa aattaactac gacgccttca tcagttatcc
     1261 agaggctgat acagttggta aatttcaatg cagattgcca gactcagtcg ccagcataat
     1321 tttaagcaga atcaacaatg tattcgaagc ccacctttca ggaagaacag actacaaaga
     1381 cagtttggga gagattgaga aactagctac tgaaaaatgg gagaccctgt ttgaaggtga
     1441 aagaacatga atgaaggagc cgtacggctc cttcattatc ttctctctgc aagttttata
     1501 gtaatacttg actgcctctc cagttgtttc agagcatcca caacagcatc accaacacgg
     1561 tctttagtac ccttgttgtt aagtctttcc aggtctctaa agagtactct tgaaaacatg
     1621 gctcttagac gaatattctc ttcaaacaaa tctttggagt catgaagagc ttcaaaaaag
     1681 tcgtctcttc tgtcatgctc tattcccata agagcgtttt ccgcatttgc ctgagaggca
     1741 aatactccaa ccttccagaa atcttctttc tgagcatcca cattggaagg aataccgcca
     1801 taatatttat taagtatctt ctgcccttcc acagactgtg aaaacccaat cagtttcgca
     1861 gccagattct tctttttgct tgaatccacc atggcaaaac catgaataaa gtccgtattg
     1921 cttgctccca catctccttc agggaatttt ggcaacggca gaatcaatag attattatcc
     1981 tttgcaagtt cttcagcata atttgcagga ttcctcatta gttcctcctc tgaaaggaaa
     2041 tacatcccgt ttctgtcagg ttgacttgca aaggcaaagg acattccgca ttttgcattc
     2101 aaactgttct tggcatcaaa tccgtttgcc tgagcgtatc ctctattcaa aagcatgaac
     2161 atactagata aaccttcaaa gcttttgccc tgtgtaagga caagatccaa attatcctca
     2221 tcaaaccact ctccgccaaa gccccgtaca aactgcatcc atacaaaggc ctggtgatat
     2281 ggcagatata ctccggtgta ggactttgag ttctttttct gtgtaagtgc ttctaccgtt
     2341 tctacaaaat cctcataagt ccaattgctg tctggtacct tgacaccaag ttcttcgaaa
     2401 acgttcaagt cacacaatac aacagctctg tcg
//
LOCUS       DAONVY010000367         2425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29435_length_2425_cov_3.070886, whole genome shotgun sequence.
ACCESSION   DAONVY010000367 DAONVY010000000
VERSION     DAONVY010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29435_length_2425_cov_3.070886"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..467)
                     /locus_tag="PLI11_08975"
     CDS             complement(27..467)
                     /locus_tag="PLI11_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPZ53038.1"
                     /translation="MRFTDSASNAINTAINKAISLGLPYVGTEHILYGIVSEAKSISG
                     KLFAKHGVTPEKLEPFLNRLAGDNVGQKVSIEQARYTPRTKRVIDNSFREASMRGQKF
                     VGTEHLWFPLFLNPIVLPLKSLLKLVWNRRSSLWILTRYLLVRR"
     gene            complement(483..1514)
                     /locus_tag="PLI11_08980"
     CDS             complement(483..1514)
                     /locus_tag="PLI11_08980"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HPZ53039.1"
                     /translation="MSGWFTKSGPMQDVVISSRVRLARNFKSLSFPTSENIKSAEESI
                     SIVKKAVDSVKNLNFKVIDLGSLPQLDQEYLVEIHMMSTDMVGKKIPTALVIKEGNEN
                     LAVMVNEEDHLRIQSLMPGLQLEDAYKQCLEFEGIAEFQPKYAFSHEIGYLTSCPTNT
                     GTGLRASAMVHLPALEKTDKMESLIDQCRKMGVAVRGFHGENTKSLGSCYQISNQVTM
                     GFTEHEIISGIDNLIKNIIQLEREARETLRSKFGITFDDMIFRAYGILTNARMLTSEE
                     ALRLLSELKLGIDLGMFAEYDTTDIIALMSNVQPASLQKISGKILSPGERDILRANII
                     REKLKSKTE"
     gene            complement(1516..2025)
                     /locus_tag="PLI11_08985"
     CDS             complement(1516..2025)
                     /locus_tag="PLI11_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPZ53040.1"
                     /translation="MLCDRCKARNATIRKIQVVNFHKQEMNLCNQCAVELGYIGNNIG
                     SLFSTLLNMDNIYNGVGAFDKKCPVCNSSINTINKTGKFGCSECYDTFNKEVRALLRK
                     IHGNCAHKGATPYVVAKNDAGESIADVSKRQMELVKLKQLMQEAVDSEEFEKAAELRD
                     RIKAIEEGE"
     gene            complement(2028..>2425)
                     /locus_tag="PLI11_08990"
     CDS             complement(2028..>2425)
                     /locus_tag="PLI11_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HPZ53041.1"
                     /translation="ELANYFNCVPSQINYVIDTRFSPEKGYYVESRRGGGGYIKICRI
                     RFDKDKNKDHYTMHVIGGMGKSLSQYHCQIFIRNFLEYEIITEREARIMEAATSDKIL
                     KFLPTVERDKVRASLLRNMLTSLLTQRGD"
BASE COUNT          648 a          589 c          389 g          799 t
ORIGIN      
        1 catgccacct acattctccc ctgacatcat ctcctgacca gtaagtatct tgttaagatc
       61 cataaggaac ttctgcggtt ccacaccaat ttcaataagg attttaacgg caatactatc
      121 gggttcagaa acaagggaaa ccaaagatgc tctgttccca caaacttctg tcccctcatg
      181 gatgcttccc tgaaactgtt atctatgact ctttttgttc ttggtgtgta tcttgcctgt
      241 tctatgctga ctttctgtcc tacattgtct ccagccagcc tgttaagaaa aggttcaagt
      301 ttttcaggtg ttaccccatg ctttgcaaaa agcttgcccg agatgctttt tgcttctgaa
      361 acgataccgt atagtatatg ctcggtaccc acatatggca atcctaatga tatcgccttg
      421 tttattgctg tatttatagc gtttgacgca ctatctgtaa atctcattca gtcaacctcc
      481 attcattctg tcttcgactt taacttttct ctaattatgt ttgctcttaa aatatctcgt
      541 tctcctggtg ataatatttt accagaaatc ttctgcaagg atgcaggttg aacattcgac
      601 ataagagcaa ttatatcagt agtgtcatat tctgcaaaca ttcccaggtc aatacccagt
      661 ttaagctccg acaaaagtct caaagcttct tctgatgtaa gcattctggc attggtcagt
      721 attccgtatg ccctgaaaat catgtcgtca aaagttattc caaactttga ccttaacgtc
      781 tctctagctt ccctttcaag ctgtattatg ttttttataa gattgtcaat gcctgaaatt
      841 atttcatgtt cagtaaaccc catggtaacc tggtttgata tctgataaca gcttccaaga
      901 ctttttgtgt tttctccatg aaaacctctt actgcaacac ccattttcct gcattgatca
      961 atcaaagatt ccatcttgtc cgtcttttcg agggcaggca gatgaaccat tgcagatgct
     1021 ctaaggcctg tacctgtatt ggtaggacag ctggtaaggt acccaatctc atgactgaaa
     1081 gcatatttgg gttgaaactc tgcaatacct tcaaactcaa gacattgctt gtatgcatcc
     1141 tccagctgca aaccaggcat caggctttgt atcctcaaat ggtcctcttc attaaccata
     1201 accgccaggt tttcatttcc ttctttaata accaaagcgg taggtatctt ttttccgacc
     1261 atatcggtac tcatcatatg aatctcgaca agatactcct gatcaagctg aggaaggctg
     1321 cctagatcaa taaccttgaa attgagattc tttaccgaat ccacagcttt tttgactatg
     1381 gatatactct cttccgcact cttaatattc tccgatgtag gaaaagacaa actcttgaaa
     1441 ttcctggcaa gtcttactct ggaacttatg accacatcct gcatcggacc gcttttggta
     1501 aaccaacccg acatattatt caccttcctc gatagccttt attctatccc ttaattcggc
     1561 cgctttttca aactcttcac tatccaccgc ttcctgcata agttgcttca gtttaaccag
     1621 ctccatctgc cttttgctaa catcagcaat gctctcgcca gcatcatttt tcgccaccac
     1681 ataaggagta gcacccttgt gcgcacaatt gccgtgaatc tttcttaaca gagcgcgtac
     1741 ttccttgtta aaggtatcat aacattctga acaaccaaat ttacctgtct tgttgattgt
     1801 attaatgctg gagttgcaaa caggacattt tttgtcaaag gccccaacac cattgtatat
     1861 attgtccatg ttcagcaaag tagaaaacaa agaaccgatg ttgttgccaa tatatccgag
     1921 ttcaactgca cactggttac acaaattcat ctcctgctta tgaaaattga caacctgtat
     1981 ctttctgata gtggcatttc ttgctttgca tctgtcacac agcattgtta atcacccctt
     2041 tgtgtcaata aagaagtaag catattcctc aataacgaag ccctcacctt atctcgctct
     2101 acagtgggca ggaacttcaa tattttgtcg cttgtagcag cctccatgat tcttgcttcc
     2161 ctttcagtaa taatttcata ctcaagaaag tttctaatga atatctgaca atggtactgt
     2221 gatagactct tgcccatgcc accaattaca tgcatggtat agtggtcctt attcttgtcc
     2281 ttgtcaaatc taatcctaca aatcttgata tatcctccgc ctccccttct gctctcgaca
     2341 taatagcctt tttcaggaga gaatcttgta tctataacat aatttatttg agaaggtaca
     2401 cagttaaaat agtttgcaag ctcat
//
LOCUS       DAONVY010000368         2425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29438_length_2425_cov_2.757806, whole genome shotgun sequence.
ACCESSION   DAONVY010000368 DAONVY010000000
VERSION     DAONVY010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29438_length_2425_cov_2.757806"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLI11_08995"
     CDS             complement(<1..206)
                     /locus_tag="PLI11_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPZ53042.1"
                     /translation="MQISVIPCLDVNRGRVVKGINFENLIDAGDPVLVGRAYSKAGAD
                     ELVFLDITATTEHRKTHLDMVRKVS"
     gene            336..851
                     /locus_tag="PLI11_09000"
     CDS             336..851
                     /locus_tag="PLI11_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53043.1"
                     /translation="MSRKELNEIEIYEKKRKIKLIIFPLLVILITPIIIFVSSRMTTL
                     SEQLNVKLPHTMIIYRARDQRSREVFDQNALKDITRMLNLMPKERVHDQRNVYMDYEN
                     AYYLTLQFEDDYGYDYTEQYLISGTYMSVSKDGVTELYKIKSKDDVDKLISSINTVLR
                     PRRNRGDWSQN"
     gene            913..1392
                     /locus_tag="PLI11_09005"
     CDS             913..1392
                     /locus_tag="PLI11_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPZ53044.1"
                     /translation="MHRILELLENNSNLTPEDIAVMIDKTPDEVRAAISQYEKEGVIV
                     GKKTIINWQKADKDLVTAFIEIKCTPQYGRGFDQIAERFYQYEQVKSVYLMSGAYDLA
                     LIVEGNSLTEVASFVSGKLAPMDTVISTCTHFVLKKYKDNGVMLETPPKDERQVMAF"
     gene            1389..>2425
                     /locus_tag="PLI11_09010"
     CDS             1389..>2425
                     /locus_tag="PLI11_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPZ53045.1"
                     /translation="MKLSPKDQINSVVANLPPSGIRKLFDIASGMDGVISLGIGEPDF
                     ITPWHIRNAGIMSLEKGRTQYTSNAGLVELKQEVANYMKRRFDLSYEPNNQVLITVGG
                     SEAIDLAIRAFVEPGDEVLIPIPCFVCYDPITRLAGGVPVLLETKAEDNFKLTPELLK
                     KNITDKTKALILPFPNNPTGAIMDKEDLEKIAEVLRDTNIMIISDEIYAELTYTDKRH
                     VSIAEIDGMYDRTVVINGFSKAYAMTGWRLGFACGHPDVIGPMIKIHQYAIMCAPTTS
                     QYAAIEALRFGDKDIEDMKTEYNYRRKLIVNGFNKMGLDCFEPMGAFYCFPSIKRTGL
                     SSEEFCEKLLL"
BASE COUNT          794 a          489 c          502 g          640 t
ORIGIN      
        1 gagacttttc ttaccatgtc caagtgagtc tttctgtgct cggtggttgc agttatgtcg
       61 agaaaaacaa gttcgtccgc accagctttt gaatacgctc tacctaccag tacaggatca
      121 cctgcatcaa tcagattctc aaagtttata ccttttacta ctcttcccct gttaacatca
      181 agacagggta tcacacttat ctgcatgggc ttttggctcc tttttaaaaa agtttacccc
      241 tttagtatag caaagcgttc atttttagta aagaaggttt acaggagaag aaagatataa
      301 tatactttag tataagaaat atggaggggt tgtttatgtc cagaaaagaa ttgaatgaaa
      361 tagaaatata cgaaaagaaa agaaagataa agctgataat cttccctttg ttggtaatat
      421 tgataacacc aataattatt tttgtttctt cccgcatgac cactctatcc gagcagctta
      481 atgtcaaact gccccacaca atgataatat acagggcaag ggatcaaaga agccgggaag
      541 tttttgacca aaacgcacta aaagacatta caagaatgct caacctgatg ccaaaagaaa
      601 gggttcacga tcagagaaat gtatacatgg attacgaaaa cgcatactac cttacacttc
      661 aattcgaaga tgattacggt tacgactaca ctgagcagta cctaatttcc ggtacataca
      721 tgagcgtatc caaggatgga gttacagaac tatataaaat caaatcaaaa gatgatgtag
      781 acaaactcat aagttcaatc aacaccgttc tgaggcccag aagaaatcgt ggagattggt
      841 ctcaaaacta gatattttct ttgatacaag gtataataga tgttaaattt tatttcaaca
      901 aggagaatca gtatgcacag aattcttgaa cttttggaaa ataacagcaa tctgacccct
      961 gaagacatag cagtcatgat tgacaaaacc cctgatgaag taagggcagc aataagtcag
     1021 tacgagaagg aaggcgtcat tgttggcaag aaaaccatta tcaactggca gaaagctgac
     1081 aaggaccttg ttacagcttt tatagaaata aaatgcacac cgcagtatgg aagaggtttt
     1141 gaccagattg cagaacgctt ctaccagtat gagcaggtca aatccgttta tttaatgtcg
     1201 ggtgcatacg atcttgctct gattgttgaa ggcaattcac ttaccgaggt tgcttccttc
     1261 gtatcaggca aacttgctcc catggacacc gttataagta catgcaccca ctttgtactc
     1321 aagaagtaca aagacaacgg agtcatgctt gaaacgcccc caaaagatga aagacaggtg
     1381 atggcgtttt gaaactctct ccaaaagatc agataaattc agttgttgcg aatttgcccc
     1441 catcaggtat tcgcaaactt tttgatattg cttcaggcat ggacggtgta atttccttgg
     1501 gaataggtga accagacttc ataacgccct ggcatattcg caatgccggt atcatgtctt
     1561 tggaaaaagg cagaacccaa tacacctcca acgccgggct tgtggaactc aagcaggaag
     1621 tcgcaaacta catgaagagg cgctttgacc tatcatatga accaaacaac caggttttga
     1681 ttactgttgg cggcagtgag gccattgacc tggcaatacg tgcatttgtg gagccaggtg
     1741 atgaggtgct gattcccatc ccctgttttg tctgctatga tccaataaca agacttgcag
     1801 gaggagtccc tgtccttttg gaaactaaag cggaggacaa cttcaaactc actcccgagc
     1861 ttcttaaaaa gaacatcaca gacaaaacaa aagcactgat tctgcccttt cccaacaatc
     1921 ccacaggagc aataatggat aaggaagacc tggagaaaat agcagaggtt ctgcgcgata
     1981 cgaatataat gataatatcg gatgagattt atgctgaact tacttacact gacaaaaggc
     2041 atgtctccat tgctgaaata gatggcatgt atgatagaac cgttgtaata aacgggtttt
     2101 caaaagctta tgccatgaca ggctggcgtc tgggatttgc ctgtggacat cctgatgtaa
     2161 tcggacctat gataaaaata caccagtatg ctataatgtg tgctccaact acaagccagt
     2221 atgctgcaat agaagctctt aggtttggag acaaggatat tgaagacatg aagaccgagt
     2281 acaattacag aagaaagctc attgttaacg gcttcaacaa aatgggactt gactgttttg
     2341 aaccaatggg tgccttctac tgctttccaa gcataaagag aacaggcctt tcatctgaag
     2401 agttctgtga aaaacttctt ttgga
//
LOCUS       DAONVY010000369         2421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29498_length_2421_cov_3.158073, whole genome shotgun sequence.
ACCESSION   DAONVY010000369 DAONVY010000000
VERSION     DAONVY010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29498_length_2421_cov_3.158073"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLI11_09015"
     CDS             complement(<1..489)
                     /locus_tag="PLI11_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53046.1"
                     /translation="MTIEPTILPTPKPTEDNDTTLPEYIGGSLSGPFESSGDTVVYKV
                     DNTTKEQYENYVEKIMQKRFENVLSNDVNGNLYNTFVKKDRLVHISYFKQSTSVIIVY
                     ETRGFLPKSVQKLNSKRKYQPLLTQIKLDSNDMLEGMSYVFRLSDGSFFIIDGGWPEK
                     EHS"
     gene            complement(755..1498)
                     /locus_tag="PLI11_09020"
     CDS             complement(755..1498)
                     /locus_tag="PLI11_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53047.1"
                     /translation="MSRVARVAKIAVTALILVLISTGCMRAEFVIELNEDGTGTLSNV
                     FAMTEEAYNLFLENGTDPFEDYTTDKKVIDGVSYVYYTEDLGKMTFKEIEEELLEFIP
                     DTDSDDPIPLFKDASIKKKASLFKNEYRFKMVTNKDAVTDNETMSASELMTVLVSVKM
                     PGKVISHKVNDNVVNITIDEYSEEKTYEIVSTSSNTLVIGILVLLLFFLIIAVVVVVV
                     IVVVLKKKKDAKVQDSTYTPAAEETDQEL"
     gene            complement(1549..>2421)
                     /locus_tag="PLI11_09025"
     CDS             complement(1549..>2421)
                     /locus_tag="PLI11_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ53048.1"
                     /translation="QMTNQIECSRFKNRPIFYTNGHEANLFGLEPNYGCCTANFNQAW
                     PKFALSTFAHNEKGIYSAVIAPAKLNTRINGVNVSIECITDYPFKNKVTYVLKAEEKT
                     RFTFGIRIPLCVESATVEGKAVEMGAMHEITREWHEDTVEVVFEFKPKLVERPNDMFC
                     LWYGPLLFALPIKEEWVGYEYTRDNVERKYPYCDYEVLPRSKWNYAFASKDFKVSING
                     IKDRPFSRNKPPVVISAEMYEINWGYEEGYDNVCRETPLSREPIGGKVEMILIPYGCS
                     NLRMTEMPLLTCCN"
BASE COUNT          702 a          528 c          368 g          823 t
ORIGIN      
        1 actgtgttct ttttcaggcc atcctccatc aattatgaag aaggagccgt cagagagtct
       61 gaacacgtat gacatgcctt caagcatatc attactgtcc agtttaattt gtgtgagcaa
      121 tggttggtat ttcctttttg aattgagctt ttgaacgctt ttgggcagaa aaccccttgt
      181 ttcgtatacg attattacac tggtagattg cttaaaatag ctgatgtgta caagtctgtc
      241 ttttttaaca aaggtattgt ataaatttcc gttaacatcg ttgctcagta cattttcaaa
      301 tcgtttttgc ataatctttt caacataatt ttcatactgc tcctttgttg tattatccac
      361 cttgtaaaca accgtgtctc cggaactttc aaacggtcct gacaatgaac ctcctatgta
      421 ttcgggcagc gtagtatcat tatcttctgt tggtttgggt gttggaagaa ttgtaggttc
      481 tattgtcata ccagggcttg gctcagtttc tgcattattt aactcaatgt atgacaagaa
      541 tagtacaaca aaacatattg acagaaataa tacaatatat tttctgaaaa catacatata
      601 cgatacgtcc tttgcttttt gtttctactt atattgtatt aaatccatct gttatattga
      661 ataaaatatg tattaacttg tgttaaaaac ccccctgaaa aacagggagg ttttgccaaa
      721 tagtttcaat tccaattatt ttgttaatta catattataa ttcctgatct gtctcttcag
      781 ctgccggagt atatgtagaa tcctgaactt ttgcatcctt tttctttttc aataccacaa
      841 caataacaac aactacaact acagcaatta tcaaaaagaa caacaacaat accagaatcc
      901 ctataaccaa agtattgctt gaagttgaaa caatctcata agtcttttcc tcgctgtact
      961 catcaatagt tatattcacc acattatcat ttactttatg ggatataacc tttccaggca
     1021 tttttaccga caccaacact gtcataagtt cacttgcact cattgtctcg ttatcggtaa
     1081 cagcatcctt gttagtaacc atcttgaatc tgtactcgtt tttaaacagc gacgctttct
     1141 ttttaattga tgcatccttg aacagaggaa taggatcatc actatcagta tctggaatga
     1201 actccaatag ttcttcctct atttctttaa aagtcatctt acccaaatct tcagtataat
     1261 aaacataact cacaccgtct attacttttt tgtccgtcgt ataatcttcg aacggatcgg
     1321 tcccgttctc caaaaaaaga ttgtacgcct cctctgtcat ggcaaataca ttcgataaag
     1381 taccggtacc gtcttcattt agctcaataa caaactcagc tctcatacaa ccagttgaaa
     1441 tcaaaactaa aatcaaggcc gttactgcta tctttgctac tcttgcaact ctgctcataa
     1501 ataggccctc caaaataaat acttgattaa ttctaccatg ctcacaaatc aattacaaca
     1561 tgttaacagc ggcatttctg tcatcctaag attgctacat ccataaggaa tcaggatcat
     1621 ttcaaccttg cctccaatcg gttctcttga aagaggagtc tctctgcata cattatcgta
     1681 tccttcttca taaccccagt ttatctcata catttccgca gatataacta caggaggttt
     1741 gtttcttgaa aaaggtctgt ccttgattcc atttatgctt accttgaaat ccttgctggc
     1801 aaaggcatag ttccattttg aacgtggaag gacctcataa tcgcaatatg gatactttct
     1861 ttcaacattg tctcttgtat actcgtaacc aacccactct tccttgattg gaagtgcaaa
     1921 aagcaaaggt ccgtaccaca agcagaacat gtcattaggt ctttccacaa gttttggctt
     1981 gaattcaaat acaacctcaa ccgtgtcttc atgccattct cttgtaatct catgcattgc
     2041 ccccatttca acggccttgc cttcaaccgt tgcactctcc acacataaag gtatgcgtat
     2101 tccaaatgta aatcgggtct tttcttcagc ttttaataca taagtcacct tgtttttgaa
     2161 tggataatcc gtaatacatt ctattgatac atttacacca tttattctcg tgttcaattt
     2221 ggctggagca attacagcag aataaatacc tttttcatta tgggcaaatg tgcttaaagc
     2281 aaattttggc catgcctggt tgaagtttgc agtgcagcac ccataattag gctccaaacc
     2341 aaaaagattt gcctcgtgtc cattggtgta gaatatgggt ctgttcttga aacgactgca
     2401 ttcaatctgg ttggtcatct g
//
LOCUS       DAONVY010000370         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29661_length_2410_cov_3.394480, whole genome shotgun sequence.
ACCESSION   DAONVY010000370 DAONVY010000000
VERSION     DAONVY010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29661_length_2410_cov_3.394480"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLI11_09030"
     CDS             <1..333
                     /locus_tag="PLI11_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPZ53049.1"
                     /translation="IKETLKDLTNRKIMVFHPAFGYFASDYELEMYSLEEEGKEATAK
                     RLEELIDLARRENIKAIFYQSEVDASQSKAFAEEIGGKAIMLEPLAEDYIKNLEKMAR
                     LIAEADNG"
     gene            326..1102
                     /locus_tag="PLI11_09035"
     CDS             326..1102
                     /locus_tag="PLI11_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPZ53050.1"
                     /translation="MAKPLIEVKDLTVYYGNTRALSNVNLEVEEGEYLGIIGPNGGGK
                     TTLLKCIAGLTKYKTGTISIKDKNIRIGYVPQFSNLDKGFPITLVEVVLSGMINPKLK
                     PFYRYAQKEKDRAYELLEKVGIKSLAKRQISELSGGEFQKMLIARALAQEPKLLLLDE
                     PTASVDAKSRSQIYKLLGRLNEEMTIILVTHDLLAISSEVRSLACLNNTLVYHGGPEM
                     NETILTQMYGCPVDLIAHGIPHRVLRHHHNGVHEGGEEEC"
     gene            1096..1938
                     /locus_tag="PLI11_09040"
     CDS             1096..1938
                     /locus_tag="PLI11_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPZ53051.1"
                     /translation="MLNSILNYQYMQNAVIASILAGIVCGIMGVIIIEKKLVMMSGGI
                     AHTAYGGVGLGYMLGIEPVFGAILFSTVASLSVGYIKRKGTAKSDVIIGMFWSLGMAL
                     GILFITLTPGYPPNISTYLFGNILSIPRFYIKIMGVLTLLILLVTVIFFNHWKVYLFD
                     DEFAYIVGIKTALLEYVLMVMIALTIVVLIRVVGIILVLALLTAPSATAALLTNNLKN
                     RMIWSSVLVILFCMTGLWISYNINLAPGSVIVIMSVGLYFIIYAVKYILKSGSVERWS
                     LKRS"
     gene            1917..2327
                     /locus_tag="PLI11_09045"
     CDS             1917..2327
                     /locus_tag="PLI11_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPZ53052.1"
                     /translation="MEFEKELRKNGLKNTPHRTRIMEVLESYEVPVSAQDIYERLKED
                     KVRISLSTVYRTLDKLVALDFVRKLSFTGEDSALFESIRKGHRHYLVCLRCRKKTVID
                     KCPIKEYQETLERETDFSIEGHKLAIYGYCPACK"
BASE COUNT          777 a          395 c          560 g          678 t
ORIGIN      
        1 ataaaagaaa ccttgaagga tcttacaaac agaaaaatca tggtttttca tcccgcattt
       61 ggttattttg cctctgacta tgaacttgag atgtattctt tggaggaaga aggcaaggaa
      121 gcaactgcca agcgccttga agagctgata gacttggcca ggagggagaa cataaaagct
      181 attttctatc agtctgaggt ggatgcaagc cagtcaaagg catttgcaga agaaatcggt
      241 ggaaaggcaa taatgcttga accccttgca gaagactata tcaaaaatct tgaaaaaatg
      301 gcaagactga ttgcggaggc agacaatggc taaaccatta atagaggtaa aggaccttac
      361 agtatattac ggaaacacaa gagcattatc aaacgtaaat cttgaagttg aagagggaga
      421 atatcttggt atcattggtc ctaatggagg gggcaaaacc accctcctta aatgcattgc
      481 agggcttaca aaatacaaga caggaacaat aagcataaag gataaaaaca taagaatagg
      541 atacgttccc cagttttcca accttgacaa ggggtttccc ataacccttg tggaagtggt
      601 tctgtctgga atgataaatc caaagctgaa gcccttttac aggtatgcgc aaaaagagaa
      661 agacagggct tatgaacttc tggaaaaggt gggtataaag agtcttgcga aaaggcagat
      721 tagcgagctg tctggtggag agtttcaaaa aatgctaata gcaagggccc ttgcacaaga
      781 accaaaacta ttacttcttg acgagcctac agccagcgtg gatgcaaaat caagaagcca
      841 gatatataag ctgttgggga gactcaacga agagatgaca attatactgg tgacccatga
      901 ccttttggca atatcatctg aagtaaggag ccttgcatgt cttaacaata cccttgtcta
      961 ccatggaggg cctgagatga acgaaaccat tcttactcag atgtatggtt gtccagtgga
     1021 tttgatagct catggcatac cgcacagggt tttgaggcat caccacaatg gagtgcatga
     1081 agggggcgaa gaagaatgct aaactcaata ttaaactacc aatatatgca gaacgcagtt
     1141 attgcaagca ttctcgcagg catagtatgc ggtataatgg gagttataat aattgaaaag
     1201 aaacttgtca tgatgagcgg aggaattgca cacactgcgt atggtggtgt aggccttggc
     1261 tacatgttag ggattgaacc tgtgtttgga gcaatattgt tttcaacagt tgcctctttg
     1321 agtgttggat atatcaagcg aaaaggtacg gcgaagtcag acgtaataat tggcatgttc
     1381 tggtcccttg gaatggcttt gggcatttta ttcataacat taacgccggg atatcctccc
     1441 aatatctcaa cgtatttgtt tggaaacata ttgagtattc caaggttcta tataaaaatc
     1501 atgggagttc taactttatt gattctgctt gttacggtta ttttcttcaa tcattggaag
     1561 gtgtatcttt ttgatgatga gtttgcatat attgttggga taaagactgc acttcttgag
     1621 tatgttctca tggtgatgat agcactgacc attgtggtac taataagggt ggttggaatt
     1681 atactcgtac ttgcgctttt gacggctccc agcgcaactg cagccttgct aacaaacaat
     1741 ctcaagaata gaatgatttg gtcttctgtt ttggttattc ttttctgcat gacagggctt
     1801 tggatttcgt ataatatcaa cctggctcca ggtagtgtga tagtaataat gagtgttgga
     1861 ctgtatttca taatatacgc agtaaaatat atattaaaat caggaagtgt tgaaagatgg
     1921 agtttgaaaa ggagttaagg aaaaacggac ttaagaacac gcctcataga acaaggatta
     1981 tggaagtttt ggaaagttat gaagttccag tatcggcaca ggatatatat gaaaggctaa
     2041 aggaggacaa ggtaagaata agtttatcca cagtctacag aactctggac aagcttgtgg
     2101 cacttgactt tgttagaaaa ctaagtttta ccggagagga ttctgccttg tttgaatcca
     2161 taaggaaagg gcacagacac tatcttgtat gtttgaggtg tagaaagaaa acggtcattg
     2221 acaagtgtcc gataaaggag tatcaggaaa ctctggaaag agagactgat ttttcaatag
     2281 aaggacataa gcttgccatt tacggttatt gtcctgcatg taaatgacaa tttaaaatca
     2341 gacaaacttt atttcatctt ggataaaaag ccatattgat ttggtatttc tttttatata
     2401 tgatttattt
//
LOCUS       DAONVY010000371         2406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29734_length_2406_cov_2.276478, whole genome shotgun sequence.
ACCESSION   DAONVY010000371 DAONVY010000000
VERSION     DAONVY010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29734_length_2406_cov_2.276478"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1215)
                     /locus_tag="PLI11_09050"
     CDS             complement(4..1215)
                     /locus_tag="PLI11_09050"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPZ53053.1"
                     /translation="MFARKVKIKVTPSTLQPTIFVLKMTEDEFYELNEWKQEEYDLYL
                     IDESTKKELQCQISDASLEATPEGNLRDYCMYILVNNLESEEEITYTFSINKRKKEEV
                     VLPNGHSYNPWGKTKGVFLENQTDNKRVIVCINGALFTRYLYSEDLPKPYFYPLIGPA
                     GKTLIHDQADDHLHHHGLWWGHDSVNGHQLYHEFRGEGRQRHVSFLSCFSGPVFGQIT
                     ALIDWQANNGERLLRETRTMRIYNMPNDSRYVDLVCQLHATEGDVEFSDTKEGGFPFV
                     RVNEEINGHHTGQILSSNNDVGESNVFGKEADWVDYTGKIKRGSNYVTTGLSVFVHPT
                     SDDYPAKWFVRDYGPFTSSNFHFCGGHTIKRGDKYTFKQRLYIHKNSPEEAFVNRRYE
                     EYKEVLKAVLT"
     gene            1375..>2406
                     /locus_tag="PLI11_09055"
     CDS             1375..>2406
                     /locus_tag="PLI11_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPZ53054.1"
                     /translation="MKRLILIHMFILVILFSSCSPDLSKAPLKDYAPRTAGSIELAGV
                     YKEKVTKRSNTVAVEELGFELTYDSVFYGHDSAINRDFMQYCRSFEEYSSSLKLKDIE
                     KIYMDSFNGDLHDDILYYKDGKVVIWEALGEMEYYTVDNVFDVGVGSNFYGTGFFNDD
                     DYVDLLFVNDKGIICILDQKDGGFEKKVYASNIRPKKEKVLAGDVNGDGLCDIVTIKG
                     SKATSYINTGKGFEKNKTKDIYLLPKNVIDVAAGDMNTDSFTDIVVVYKEGRRYFIRT
                     LFGRGNGGFGPTEKEKSTRYYKNLFGEYAIESISNIVSVSTGDANGDRIPDVALITNY
                     KNHIYLNWLL"
BASE COUNT          685 a          437 c          522 g          762 t
ORIGIN      
        1 ttttcatgtc aaaactgctt ttaaaacttc tttgtactcc tcgtagcgtc tgtttacaaa
       61 tgcttcttca ggagaatttt tgtgtatgta gagtctttgt ttgaaggtgt atttatctcc
      121 tctctttatg gtatggccac cgcagaaatg gaagttggat gaggtaaaag ggccatagtc
      181 tcttacaaac catttggcgg gataatcgtc cgaagttgga tgtacaaaga ctgaaagacc
      241 tgttgttaca taattagaac ctcgtttaat ctttcctgta tagtcaaccc agtctgcttc
      301 ttttccaaaa acattacttt ctcccacatc attatttgag gaaagtattt gtcctgtgtg
      361 atgaccgttt atctcttcat ttacacgtac gaagggaaag ccgccttctt ttgtgtcact
      421 gaattcaaca tcaccttcgg ttgcatgcag ctggcagaca agatcaacat atctcgaatc
      481 gtttggcata ttatatatcc tcatagttct ggtttcccgg agaagtcttt cgccattgtt
      541 ggcttgccag tctatgaggg cggtgatttg accgaaaacc ggtccgctaa aacaggaaag
      601 gaaactaaca tgcctttgcc ttccttcgcc tctgaattca tgatacagct gatggccgtt
      661 gacactgtcg tgtccccacc acaacccgtg atgatgaaga tgatcatcgg cctggtcgtg
      721 tatcagtgtt tttcctgcag gaccaatcag tggatagaag tagggtttgg gcaaatcttc
      781 ggaataaaga tatcttgtaa acaaggcacc atttatgcat actattactc ttttgttatc
      841 tgtctggttt tcaaggaaaa cgccttttgt tttgccccag ggattgtatg agtggccatt
      901 gggaagcacc acttcttctt tttttctttt gttaatactg aaggtgtatg tgatctcttc
      961 ttctgattcc aggttgttta caagtatgta catgcaataa tctcgaaggt tgccctctgg
     1021 tgtcgcttca agtgaagcat cacttatctg gcattgcagt tctttttttg tggattcatc
     1081 aatcaagtaa aggtcgtatt cttcctgctt ccattcattc aactcataaa actcatcttc
     1141 tgtcattttt aaaacaaaaa tggttggctg cagtgttgac ggagtaactt ttatcttgac
     1201 tttccttgca aacatttcaa acctccttta ttttttgact atactaacag gtagttttaa
     1261 agttttacat ggaatatatg tcttttattt atactaaata gcgacaatct tgcagaaaag
     1321 tttttcctca tgatatactt gaatcaacca aaacgataaa aggagtgctt tccattgaaa
     1381 agactcatac tgatccacat gtttatccta gtgatactgt tttcatcatg cagtccggac
     1441 ctttccaaag cacctttgaa ggattatgca ccaagaactg cgggcagtat agagcttgca
     1501 ggtgtttaca aggagaaagt gaccaaaaga tcaaacactg tagcagtgga ggaacttggt
     1561 tttgaattga cctacgacag tgtgttttat ggtcatgatt ctgcaattaa cagggatttc
     1621 atgcagtact gcagaagctt tgaggagtac tcaagcagtc tcaagcttaa ggatatcgag
     1681 aagatttata tggattcctt caatggagat ctgcatgacg acatcctgta ctacaaggat
     1741 ggcaaggttg taatctggga agctcttggc gagatggagt actacacggt ggacaatgtt
     1801 tttgatgtgg gtgttggtag taatttttat ggaacgggtt tctttaatga cgatgattat
     1861 gtggatttat tgtttgtcaa cgacaaagga ataatctgca tattggatca gaaagacgga
     1921 ggctttgaga agaaggtgta tgccagtaat attcgaccca agaaggaaaa ggttctggca
     1981 ggagatgtaa atggtgatgg tttgtgcgac atagttacca taaaaggcag caaggcaaca
     2041 tcgtacataa ataccggtaa gggttttgag aagaacaaga caaaagacat atatctttta
     2101 cccaaaaatg tgatagatgt tgctgcagga gatatgaata cggacagttt taccgatatt
     2161 gttgttgtat acaaggaagg aaggcgttat tttataagga cgctttttgg gagaggaaat
     2221 ggtggctttg gtcctaccga aaaggagaaa agcacaagat attacaaaaa cctttttgga
     2281 gaatatgcga tagaaagcat cagcaatatt gtatccgtat caacgggcga tgccaacgga
     2341 gacagaatac cggatgtagc cttgattacg aattacaaaa atcacatata tttaaactgg
     2401 ctgttg
//
LOCUS       DAONVY010000372         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29753_length_2405_cov_2.520000, whole genome shotgun sequence.
ACCESSION   DAONVY010000372 DAONVY010000000
VERSION     DAONVY010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29753_length_2405_cov_2.520000"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..687)
                     /locus_tag="PLI11_09060"
     CDS             complement(109..687)
                     /locus_tag="PLI11_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53055.1"
                     /translation="MTKDINLYKALKKSKVKKEKNPTGVLLAIFPILIVMIMGGMSYW
                     LYVKNQELSSEIDKINEYLNNPEISKKHEEVVEIKDRQSELNDKESALIKFRNNYNSY
                     PFFNSELFEKIEGCMKDDIVIHDISYSSNVGGAYMVLQVSTTDIKAASTFTQDLSATQ
                     LFEEVYYDGWSQIRENEYTFKVTCLLKAGVHE"
     gene            complement(708..1610)
                     /locus_tag="PLI11_09065"
     CDS             complement(708..1610)
                     /locus_tag="PLI11_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53056.1"
                     /translation="MISVHISNEKIHIVNGDAKFGVFNIYEYLNAPTPDGSMLNGVIT
                     DALALREEIGDIWKTNKFPTKGISLVIDNAPVVGKVMRVPLVSHKKILRIINRELSPQ
                     EGENVLDYAVLHADDNIKKKDQKKYGMDIYACSVQKSLLQSYVDLFESNGIRITNINL
                     GINSMIKIGHFLRSLREKTYIVITLDGSSFASYLFVNGKYSISSRYRLIYDRGTNLSF
                     MEITQHVSNFLQFASTQRSGHNVENVYFCGLYKDEEALYSQINSALGIKAQIFPHNET
                     EVFVHKKAQGFILSDNIYASGNLI"
     gene            complement(1619..>2405)
                     /locus_tag="PLI11_09070"
     CDS             complement(1619..>2405)
                     /locus_tag="PLI11_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPZ53057.1"
                     /translation="SGTLDKTAMNLADHYEKDEELRLKILGSTVYPIILFGLTIVSVF
                     IIFVFIMPRFLDVFTGMELPLPTKIVLGLSSMLRNNLLAIFIIFMSIIFVFAIAWRFR
                     EFQVLFDKAKFKIPRIGKLLRIIHTARFARTLNSLYSSGIPMIKCLSIAKNTITNRFI
                     ADQFDRVIERVKSGDSLSTSIGMVDGFDIKLSSTISVGEETGRLDKMLEATANSMEYE
                     AEAAMQRITTYLAPALVIFLAVVIGFVVMAVMLPMVNLYRYRY"
BASE COUNT          668 a          525 c          394 g          818 t
ORIGIN      
        1 aaaggggctt tactacaaac attccaatac cataaaggat aaggatgacc agcaggatgt
       61 ataatagaac tttttctctt tttgtgagct ttctaaaaat cgttgacatt attcatgcac
      121 ccctgctttc aataggcacg taaccttgaa tgtatattca ttctccctga tttgactcca
      181 accatcataa tatacttcct caaaaagctg cgtggcgctc aaatcctgag tgaatgttga
      241 tgccgcttta atgtccgttg ttgatacctg caataccata tatgcgccgc caacatttga
      301 actgtaggaa atgtcgtgta ttacaatatc atccttcatg catccttcta tcttttcaaa
      361 cagttccgaa ttgaaaaatg gatatgaatt ataattgttt ctgaatttta tcaatgcact
      421 ttctttatca tttagttcac tttgcctatc ctttatttca actacttcct catgtttttt
      481 tgatatctcg ggattgttca aatactcgtt tatcttgtca atctcactgg aaagctcttg
      541 attctttaca taaagccaat atgacattcc tcccattatc attacgatta atatggggaa
      601 aatcgccaga agtacacccg ttggattctt ctccttcttt acttttgatt tttttagtgc
      661 cttgtacaga ttaatatctt ttgtcatatc gcccatccta ccccaactta tatcagattc
      721 ccacttgcgt atatgttgtc cgaaagaata aagccctgtg cctttttatg cacaaagacc
      781 tcggtttcat tgtgcggaaa aatctgcgct tttattccaa gcgcactgtt tatctggctg
      841 tatagagctt cctcgtcttt atataggccg cagaaataca cattttcaac attatgacca
      901 ctgcgctgcg tactggcaaa ctgcaaaaag tttgatacat gctgcgtaat ttccataaat
      961 gacaggttcg ttcccctatc gtaaataagc ctgtatctgc tggatatgga atactttccg
     1021 tttacaaaca aatagcttgc aaaactgctt ccgtccagtg taatgactat gtaagtcttt
     1081 tccctcaagc ttcttaagaa atgtccaatc ttgatcatgc tgtttatacc aagatttatg
     1141 tttgttatac gtatgccatt gctttcaaac aagtccacat agctttgaag aaggcttttc
     1201 tgtacactgc aagcatatat gtccatgcca tatttcttct gatctttttt ctttatgttg
     1261 tcgtctgcat gcaaaaccgc gtaatccagt acattttcgc cttcttgagg agacaactcc
     1321 ctattaataa tccgcagtat ctttttgtgg gaaaccaatg gaaccctcat tacctttcct
     1381 actacaggtg cgttatctat aacgaggctg atgcctttgg ttggaaactt gttggtcttc
     1441 catatgtctc caatctcctc ccttaatgcc agggcatccg ttataactcc atttaacatg
     1501 gagccatcag gtgtgggtgc attaagatat tcatatatat tgaatacacc aaacttggca
     1561 tcaccattca ctatatgaat tttttcattt gatatgtgta ctgatatcat tctacacctc
     1621 aatacctgta tctgtataag ttcaccatgg ggagcattac tgccataact acaaatccta
     1681 tgacaactgc caggaaaatt accaatgcag gagccagata tgtagttatt ctctgcatgg
     1741 ctgcctccgc ttcgtattcc atcgagtttg ccgttgcttc aagcatcttg tccagtcgac
     1801 cggtttcctc tccaactgat attgttgatg atagtttaat gtcgaatccg tctaccatgc
     1861 ctatcgaagt tgacaaggag tcaccacttt tcactctttc aatgacacgg tcaaactgat
     1921 ccgctataaa tctgtttgtt atggtatttt tagcaatcga caaacatttt atcataggta
     1981 ttccactgga gtaaagggag ttcaatgttc ttgcaaatct ggcagtatga attatcctta
     2041 ggagtttgcc tattctgggt attttgaatt ttgctttgtc aaacaaaacc tggaattccc
     2101 gaaatctcca cgctatagca aatacaaaga ttatagacat gaatattata aaaattgcca
     2161 gcagattatt tctgagcata ctacttaatc ccaaaactat tttggttggc agtggcagct
     2221 ccatgccggt aaaaacgtct aaaaatctgg gcattatgaa tacgaaaata atgaaaacac
     2281 ttacaattgt aagtccaaac aaaattattg gatataccgt acttcccaat atcttcaggc
     2341 gaagctcttc atctttttca tagtggtctg caagattcat agcggtttta tcaagggtac
     2401 cgctt
//
LOCUS       DAONVY010000373         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29867_length_2397_cov_3.595645, whole genome shotgun sequence.
ACCESSION   DAONVY010000373 DAONVY010000000
VERSION     DAONVY010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29867_length_2397_cov_3.595645"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..>2397)
                     /locus_tag="PLI11_09075"
     CDS             complement(88..>2397)
                     /locus_tag="PLI11_09075"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPZ53058.1"
                     /translation="IFLKDLDKEHNVITCFKKNFEVLEDVKNASISITARSFYRLYVN
                     DVFVMHGPARTAHKFLRVDDIDISLHVKKGMNEICIYVISYSDCFNGYSNDCTLERGL
                     LLFELALDDRKIVSDRTVKGYRMIKRINAGRISHSRGAGEIYVIDDKMYEEHEVEELE
                     LDAFFLERGMEYPELDRIDALKLLEFGSFVYDDNINVPQEFYEPGYKEYYDGLEYRPD
                     KDARRFASFPSRGKFNVGNGECEIIPGEDENPYVIFDFGKSHVGFFSLDLEIESCEED
                     AFCDIHHSEAMDLTGNMSIQTGGSIRLFLKKKNISFISMEPYLARYIRLCFKGVKRIV
                     VRNVSIYTYYYPDSNMGFFRCSDEDLNRLYDAARHTLLLNTLDIFMDCPERERGGWLC
                     DSLWTARSASVMLGDTSVEKAFIENFLLTPSDEMWNAFFPEVYPANKRSYKEMTGITT
                     WSFWLMIEMCEYVERTNDWEFAEKFRQRIFDFVKGSKVFLGECGLLTNMPHVFVDWSK
                     SNHYTQPISVSANSLYSYMLLRLGKLYGCSEFVELGLSIRSILRETVKDEKYIPDTLE
                     YVDGKLRCKGNYSEANQYTVLWSELFDKDEIKELAFKVVHAMGPYPTYPKDPYVDEAG
                     LFIGLCIRLDLLSKWKEYDKMLEDMKAIFYPQLKEGPGTLWENRTIDTSSRCHGFASH
                     VGVHLTRDVLGLGIPDEVDKTITISPNPNGLMWAKGIVRTKQGFASLSWHIDGDVFNV
                     KCSVPKGYKVLLDLSRNLQCYKLEIEIIQ"
BASE COUNT          693 a          588 c          383 g          733 t
ORIGIN      
        1 gcctgataaa agcactacaa caaaagcaaa aataaaaaca ataacaaata acatatgttt
       61 tctcatgctt tcacctttcc agatagatta ctgtatgatt tcaatctcaa gcttgtagca
      121 ttgaagattt cttgaaaggt ccaataaaac cttgtagcct ttgggaactg agcatttaac
      181 attaaataca tctccatcaa tatgccatga aagggatgca aacccttgtt tggtcctgac
      241 aatccccttg gcccacatca aaccattggg attcggactt atggttatgg ttttgtccac
      301 ctcgtcaggt atccccaagc ccagtacatc tcttgtcaaa tgaacaccca catgcgaagc
      361 aaatccatga catctgctgg aggtgtcaat tgttctgttt tcccatagtg tacccggccc
      421 ctccttcaac tgaggataga atatggcttt catgtcttca agcatcttgt catattcctt
      481 ccactttgaa agaaggtcga gcctgatgca aagacctatg aacaatcctg cttcgtccac
      541 atatgggtct ttgggatatg tgggataagg ccccattgca tgaaccactt taaaagcaag
      601 ctcctttatc tcatccttgt caaacagctc cgaccaaagc accgtatact gatttgcctc
      661 ggaataattg cctttacatc ttaatttgcc gtccacatac tccaatgtat cgggtatgta
      721 tttttcgtct ttaacagttt cccgtaatat actccgtata ctcaaaccca gttcaacaaa
      781 ctcactgcaa ccgtaaagct tgcccagtct tagaagcata tatgaataca aactgttggc
      841 cgatactgaa attggctggg tgtagtgatt ggatttggac cagtcaacaa acacatgggg
      901 catgtttgtc aagagtccgc attctcctaa aaataccttg gatcccttaa caaagtcaaa
      961 tattctttgc ctgaactttt ctgcaaactc ccaatcattg gttctctcca cgtattcaca
     1021 catttcaatc atcagccaga aggaccaggt tgttattcct gtcatctcct tgtaactgcg
     1081 tttgtttgca gggtatacct cggggaaaaa ggcgttccac atttcatccg aaggggttaa
     1141 caggaagttt tcaataaagg ccttttcaac acttgtatca ccaagcatga cacttgcact
     1201 tcttgcagtc cacaacgagt cgcacagcca tcctcctctc tccctctcag gacaatccat
     1261 gaaaatatcc agtgtgttta aaagcagcgt atgccttgca gcatcataca acctgttcaa
     1321 atcttcatca ctgcatctga aaaaccccat gtttgagtcg ggataataat atgtatatat
     1381 tgacacattc cttacaacta tccgttttac acccttaaag cacagtctga tatatctggc
     1441 aaggtaaggt tccatggata taaaggagat gtttttcttt ttcaaaaata atcttattga
     1501 cccgcctgtt tgtatggaca tattacctgt caaatccatt gcttcggaat gatgaatgtc
     1561 acaaaaagcg tcttcctcac aactttctat ttccaaatca agtgaaaaga accctacatg
     1621 gcttttccca aagtcgaata tgacatatgg attctcgtcc tctccgggaa ttatttcaca
     1681 ctcaccatta ccaacattga attttcctct tgaaggaaaa cttgcaaacc ttcttgcgtc
     1741 cttgtcaggc ctgtattcca aaccgtcata atactccttg taacctggct cataaaattc
     1801 ctgtggcaca ttgatattgt catcatatac aaaggaccca aattccaaca gtttcaaggc
     1861 gtcaatccta tctagttctg gatactccat tcctctttcc aggaaaaagg catccagttc
     1921 gagttcctca acttcatgtt cttcatacat cttgtcgtca atcacatata tctctcctgc
     1981 gcctcttgag tgggatatcc tgcctgcatt tattctcttt atcatcctgt atcccttgac
     2041 ggttctatca ctgacaatct ttctgtcatc cagtgcaagt tcaaacaaca aaaggccccg
     2101 ttccaacgtg caatcattgg aatagccgtt gaaacaatcg gaataactga tgacataaat
     2161 gcagatttca ttcatacctt tttttacatg caaacttata tcaatatcat ccacgcgtaa
     2221 aaacttatgt gccgttcttg caggaccatg cattacaaac acgtcgttta catacaacct
     2281 gtaaaacgac ctggcagtaa tacttatact ggcgtttttt acatcttcca acacttcaaa
     2341 attctttttg aaacatgtaa ttacattgtg ctctttatca agatccttca aaaagat
//
LOCUS       DAONVY010000374         2395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29918_length_2395_cov_3.392735, whole genome shotgun sequence.
ACCESSION   DAONVY010000374 DAONVY010000000
VERSION     DAONVY010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29918_length_2395_cov_3.392735"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLI11_09080"
     CDS             <1..532
                     /locus_tag="PLI11_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53059.1"
                     /translation="FNLMIVDKSSSISIYANEKLVCTVEFSGITKYENSKSIHADEGD
                     NSIYRDPISEFFEKAVVKDGEGKQIANLTNTLLAVNGYLALSTRHSGQVDVAEIVIKS
                     TSEKPQETSTEEPEKTPAPTDNEDDSTPIPTQTKETGSEKDKRRSVGLIAGIVIVAIA
                     AVACVVLLMVIKKKKV"
     gene            646..1542
                     /locus_tag="PLI11_09085"
     CDS             646..1542
                     /locus_tag="PLI11_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53060.1"
                     /translation="MMRKICCILVLFAIIVLNVSANAENIIGKDFEVHFKDGNWDYDV
                     IMDGDKVKSVTVRNEDETVASEAVFYIPNMSKGFVWSGDLKINQFYAEDKDGLGFIYG
                     PFFKVGAAYDEATGKEVYVHLLLTRNYGIAVEQSGVEKIQDIYSFKGETEATSKFGPM
                     SNGTKFHFEIIRDDNKLSMKINDVQILDMDLTNCDKPGDFNHFTEGYEFNLGMMGHYC
                     SFTIENMTVVNLYEPEVTPTPEPTEEVKETPKPADNDEEKTPTKEKTKDKNATGVIIS
                     AVSAIAVLLIVVFVVIAKRRKK"
     gene            1542..>2395
                     /locus_tag="PLI11_09090"
     CDS             1542..>2395
                     /locus_tag="PLI11_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53061.1"
                     /translation="MKKATAFLVISTIMVGSMFGCNRKYNYDDITTWKKYKGEYVDIN
                     GTTFCIDDLGRAVALDVERKDREVGIFYFLWTGSHGSHQVFDNTEILKKNPDAPRSKK
                     EWVKAGGSPLYGASHWWGKPLFSYYTMLDDYVIRKHVQMFVEADIDYLMIDATNTYIY
                     EEQSLLLFRILDEYSRQGWKVPKVSYYTHTNTGATIERIYNDIYMAHPEYEHLWYCYD
                     GKPLIVGDMRDSSISKRAKEFFTMKDSYMNAFEVLDVMPDGSFKYDLNKKKNMFPWID
                     FSDWPTEM"
BASE COUNT          811 a          378 c          537 g          669 t
ORIGIN      
        1 tttcaatctt atgatagtgg acaagtcatc ttcgatttcc atatatgcca atgaaaagct
       61 ggtatgtacc gtagaattta gtggtattac aaagtacgaa aattccaagt ccattcatgc
      121 agatgaagga gataactcaa tatataggga ccccatcagt gagttctttg aaaaggcagt
      181 tgtaaaggat ggggaaggca agcaaattgc aaacttaacc aacacactgt tagctgtgaa
      241 tggttatctt gctctttcta cgagacactc aggacaggtg gatgtggcag aaattgtgat
      301 aaaatctaca agtgaaaaac cacaagagac ttcaactgaa gagccagaga aaacacctgc
      361 gcctacagac aatgaggatg actcaactcc tattcccacg caaacaaaag agacgggcag
      421 tgaaaaagac aagagaagaa gtgtcggttt aatagctggt attgtgattg ttgctatagc
      481 agcagttgct tgtgttgttc ttctgatggt tatcaaaaag aaaaaagtat aaaacacaat
      541 tatttacata tggaaaacaa tatattacct acaacatttc acccatttac ccatttaatg
      601 tattatacat ataacatacc tgtttctgga ttggagggta actgcatgat gcggaaaata
      661 tgttgcatat tggtactgtt tgcaattatt gtgcttaatg tatcagcaaa tgctgaaaac
      721 attatcggca aggattttga agttcatttc aaggatggca actgggatta tgatgttata
      781 atggatggag acaaagttaa aagtgttact gttaggaatg aagatgaaac agttgcttcc
      841 gaagctgtat tctacatacc taatatgtca aagggttttg tatggtccgg tgatcttaag
      901 ataaaccagt tctatgctga agacaaggat ggactaggat tcatatatgg accgtttttt
      961 aaagtcggag ctgcttatga tgaagcgact ggcaaagaag tatatgttca tctactccta
     1021 acaagaaact atggtattgc tgtcgagcag agtggtgtag agaagattca ggatatatac
     1081 agtttcaaag gagaaacgga ggctacaagc aagtttggcc ctatgagtaa tggcacaaag
     1141 tttcattttg agataatcag ggatgacaac aaactctcca tgaagataaa tgatgtccaa
     1201 atattggata tggatttgac caattgtgat aaacctggag atttcaatca ttttactgaa
     1261 gggtatgagt tcaatcttgg aatgatgggc cactactgct cttttactat agagaatatg
     1321 acggtggtta atctatatga acctgaggtt acgccaactc ctgaacctac tgaagaagtt
     1381 aaagaaacac caaaacctgc tgataatgat gaagagaaaa ctcctacgaa agagaagacc
     1441 aaagacaaga atgcaacagg agtaattatt tctgctgttt ctgctattgc tgttttgtta
     1501 attgtggtct ttgttgtgat agcaaaaagg aggaaaaaat aatgaaaaaa gcaactgctt
     1561 ttctcgttat atctacaatt atggtgggaa gtatgtttgg ctgcaatcgc aaatacaatt
     1621 atgatgacat tacaacatgg aaaaaataca aaggagaata tgttgatata aatggtacaa
     1681 ccttctgtat tgacgacctc ggtcgagcag tagctttgga cgtagagaga aaagatcggg
     1741 aagtaggaat attctatttt ttgtggacgg gctcccatgg ttcgcatcag gtgtttgaca
     1801 ataccgaaat tttgaagaaa aatcctgatg cgcctaggtc taagaaagaa tgggttaaag
     1861 caggagggtc gcctctgtat ggagcaagcc attggtgggg aaaacccttg ttcagctact
     1921 atacaatgct ggatgattat gtaataagaa agcatgttca gatgtttgtt gaggctgata
     1981 tagactacct catgatagat gccacaaata catacattta tgaggagcaa tcacttctct
     2041 tgttcaggat acttgatgag tactcaaggc aaggctggaa ggttccgaag gtaagctatt
     2101 atacacatac aaacacagga gcaacaatag agaggatata caatgacata tacatggcac
     2161 atcccgagta tgagcatctt tggtactgct acgacggcaa acccttaatt gtaggggata
     2221 tgagggattc tagtatcagt aaaagggcaa aggaattctt cactatgaag gattcataca
     2281 tgaatgcatt tgaagttttg gatgttatgc cagatggttc ctttaagtat gatcttaaca
     2341 agaagaaaaa catgtttccg tggatagatt tttcagactg gccaacggag atgaa
//
LOCUS       DAONVY010000375         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_29954_length_2393_cov_2.279726, whole genome shotgun sequence.
ACCESSION   DAONVY010000375 DAONVY010000000
VERSION     DAONVY010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29954_length_2393_cov_2.279726"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1206)
                     /locus_tag="PLI11_09095"
     CDS             complement(79..1206)
                     /locus_tag="PLI11_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPZ53062.1"
                     /translation="MFNDIESIRKGFRNLNEDNYSNQFEFEKAREIGLCLLGRAERML
                     KEQIPKGRFEWVDDSYGSPEDIIQQAKDILKQLKDNRIPYEGRFAELGGTVIDHALIE
                     KDGVHHLFYIRGKAVTTWAECQTNNFGHAVSTDLIHWEVKEPVLQTKEGEWDEYQVWA
                     PHVIYHEGEYWMFYTGVNNNVAQAIGLAKSKDLYNWERHENNPVITPGKWAIWNKDTW
                     SDGRDPCILKDGDTFYAYYCAHMYSEEYKRNIYAIGVASSQNLYDWKDEGYFVLKESI
                     NTPPESPFVVKKDNKYYLFYTDYKAGTVFAVSDKPTTGFVDADPGIRTIIPSVSASEI
                     YFSKGKWYISYISHEPNQLHFFEIKELVWNADGSFSTKTIE"
     gene            complement(1362..1574)
                     /locus_tag="PLI11_09100"
     CDS             complement(1362..1574)
                     /locus_tag="PLI11_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53063.1"
                     /translation="MKNKEIEKILVKFVESGWDLIAVPSRKYLDGTGDLDELREAVRQ
                     ADAECGSCGCLFDELYKKALVLLENE"
     gene            1770..2354
                     /locus_tag="PLI11_09105"
     CDS             1770..2354
                     /locus_tag="PLI11_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPZ53064.1"
                     /translation="MKKWKCTVCGYIHEGDSPPVECPICKAPSTDFVLMDEKPKRWRC
                     TVCGYIHKGDEAPDTCPICGVEKGRFELVEAAAKDEYKQLDAVGPNGKKLWQCTVCLY
                     IYEGDEPPAFCARCWAPKKLFVDMKNPKPIPDPNKPAATDETEADIIVVGTGAAAFSA
                     AITARVLGNSVIMLEKSGIIGGTTRRSGGGFGHR"
BASE COUNT          673 a          573 c          453 g          694 t
ORIGIN      
        1 gtcaagcttt ccatatctcc ttaaaagcat tttcacttat ataatctctg agaagggctg
       61 tttgtgacac taatttattt attctattgt ttttgtcgaa aatgatccat cagcattcca
      121 cacaagctcc tttatctcaa agaagtgcag ctgatttggc tcatgggaaa tataggagat
      181 gtaccatttg cctttgctga aatatatttc gctggcagaa accgatggaa ttattgttct
      241 gatacccgga tccgcatcaa caaacccagt ggtcggcttg tcagagactg caaacacggt
      301 gccggccttg tagtctgtat agaacagata gtatttgttg tctttcttta ctacgaaagg
      361 tgactccggt ggtgtgttta tactctcttt aagaacaaaa tatccttcat ccttccagtc
      421 gtacagattt tgggatgatg caacgcctat tgcgtaaatg tttcttttgt attcctcact
      481 gtacatgtga gcgcaataat aggcgtagaa ggtgtcacca tctttcaaaa tacatggatc
      541 tcttccatcg gaccaggtgt ccttgttcca aatcgcccac ttcccaggag ttatcaccgg
      601 gttgttttca tgcctctccc agttgtacag atcctttgat tttgcaagtc ctattgcctg
      661 ggccacattg ttgttcacgc ctgtatagaa catccaatac tcaccctcat gatatatcac
      721 gtgaggcgcc catacctggt attcatccca ctccccttct ttggtctgca gcaccggttc
      781 ttttacttcc cagtgtatca ggtctgtact tacggcatgc ccaaagttgt tggtttgaca
      841 ttcagcccat gtagtcactg ccttgcctct tatgtaaaac agatgatgaa caccatcctt
      901 ttctatcaag gcatggtcta ttacagttcc accaagctcg gcaaatctgc cttcataggg
      961 tatcctgtta tccttaagct gcttgagtat gtcctttgcc tgctgaatta tatcttcggg
     1021 agagccatat gaatcatcaa cccattcaaa ccttcctttg ggtatttgct cctttaacat
     1081 cctctccgcc ctgccaagca gacacaaccc aatttctcta gccttttcaa actcgaactg
     1141 attggaatag ttgtcctcgt tcaaattacg aaagcccttt ctaatgcttt caatatcatt
     1201 aaacatccaa attcactccc attaattcat gtcaatttga aaatagtata catgagcaga
     1261 agaccgaagt caatcttgtg aaatagcaaa aacagtaagc tgtgtacatc ccccctttgc
     1321 agccctctca actatatagt cgctaaaaaa gcaaaaaggg ttcactcatt ttccagcaaa
     1381 accaatgctt ttttgtacag ctcgtcaaat aaacacccgc aactgccaca ttctgcatct
     1441 gcctgtctga cagcttctct caattcatcc aaatcccctg tcccgtcaag atacttccgg
     1501 gacggaacag caatcaagtc ccatcctgat tcaacaaact tcaccagaat cttctctatc
     1561 tctttattct tcattcctaa cacctccatt ctactcattg gtataattat actcctccag
     1621 gactttcatt caataaatat ataatttgac aatcacctca tattgtagaa taatgcaatt
     1681 agaagaacgt ttttgttaat aatagactca atggaccatc ttcaaaatcc aatagattaa
     1741 taatatacgg aaaaggagtc gattctgtta tgaagaagtg gaaatgcact gtatgtggtt
     1801 acatccatga aggagatagt ccccctgtcg aatgccccat ctgcaaggca ccttcaacag
     1861 attttgttct gatggatgaa aaaccaaaaa gatggcgctg cacagtgtgc ggctacatcc
     1921 acaaaggtga tgaagctccc gatacatgcc caatttgcgg agtggaaaag ggcagatttg
     1981 aattggtaga ggctgcagca aaagatgagt acaaacagct ggatgcagtt ggcccaaacg
     2041 gtaaaaaact gtggcagtgt acagtatgct tgtacatcta cgaaggagac gaacctcccg
     2101 cattttgcgc acgctgctgg gctcctaaaa agctgtttgt agatatgaag aatccaaaac
     2161 ccatacctga tccaaacaaa cctgctgcta ctgatgaaac cgaggctgac attattgtag
     2221 tcggcacggg tgcggctgca ttcagtgccg ccatcactgc aagagttttg ggcaattcag
     2281 ttatcatgct tgaaaaaagc ggaattatag gcggaaccac aaggcgttca ggcggcggtt
     2341 ttggacaccg atgaaccgtc atcagaagtc aaaagggata gaagacaaca ggg
//
LOCUS       DAONVY010000376         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_30143_length_2381_cov_3.385641, whole genome shotgun sequence.
ACCESSION   DAONVY010000376 DAONVY010000000
VERSION     DAONVY010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30143_length_2381_cov_3.385641"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLI11_09110"
     CDS             complement(<1..562)
                     /locus_tag="PLI11_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPZ53065.1"
                     /translation="MNIGFDNELYIKKQTEHIKERMRKFGNKLYLEFGGKLFDDYHAA
                     RVLPGFDVNGKIRLLKEFKDNAEVIFCINANDIEKSKIRADIGVTYDQDLFRLVDSMR
                     ESGLYVNSVVITQYNGQHSADIFRNKLERRGVRTYIHYPIAGYPGNVDLIVSEQGYGK
                     NEYIETTKPLVIVTAPGPCSGKLATCL"
     gene            774..1481
                     /locus_tag="PLI11_09115"
     CDS             774..1481
                     /locus_tag="PLI11_09115"
                     /inference="COORDINATES: protein motif:HMM:NF022599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HPZ53066.1"
                     /translation="MKRAFVYLVFSIFVCVAYFQNNASESVVDYFLEIALECEYGDGS
                     GVIKKWKMPINIYVMGNFTREDYEVIKGHVDYLNSIEGIPRVKLVRDMNEANVYVNFI
                     TQWEMSQKVCVDEIVWGFVRCFWNERYEITNAEILIVYDRTNQCQRHHVILEEMTQML
                     GLLNDSDLHEDSIFYNGYSENTRLSRIDELVIRILYNSGVKSGMRGDDARPVVSNWLV
                     YGYKLKNSSGCPFRASS"
     gene            1729..>2381
                     /locus_tag="PLI11_09120"
     CDS             1729..>2381
                     /locus_tag="PLI11_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HPZ53067.1"
                     /translation="MARKLFTSESVTEGHPDKICDQIADNILDALLKEDPESRVACEV
                     TATTGLIHIMGEITTKAFVDYEKIARNTLRNIGYTSSEIGFDADTCAVINTVNAQSED
                     IAMGVDRSYESKVEGAEVEHGAGDQGLMFGFACNETPELMPLSLVLAHKLCKRLAQVR
                     KEGILPYLRPDGKAQVTVEYDENDKPFRVDTVVVSSQHAEDVDNETIRKDIINEVVL"
BASE COUNT          681 a          414 c          524 g          762 t
ORIGIN      
        1 acagacatgt tgcaagtttt ccgctgcagg gtcctggtgc ggttactata accagaggtt
       61 ttgtagtctc gatatattcg tttttaccat aaccctgctc gcttactatg aggtcgacat
      121 ttcccggata tcctgctatg gggtagtgga tatatgttct tacacctctt ctttcgagct
      181 tgtttctgaa aatgtctgct gaatgttgtc cgttgtactg ggttatgact acactgttta
      241 cataaagacc tgattctctc atggaatcga ccagtctgaa aaggtcctgg tcataagtta
      301 cacctatatc cgcccttatt ttactctttt ctatgtcatt ggcattaatg cagaatatga
      361 cttcggcatt gtctttgaac tccttcagca aacgtatttt tccgtttaca tcaaaaccgg
      421 gaagaactct tgcggcgtgg taatcgtcaa agagtttccc cccgaactcc aaataaagct
      481 tgttgccaaa ctttctcatt ctctctttta tgtgctctgt ttgtttcttg atataaagtt
      541 cattgtcaaa tcctatgttc attagttttc ctcaattcac atttatattt taaacctatt
      601 tatgttatca tttctggact gatttttcaa catagcctaa ttcagtatag cagttaaata
      661 tgcttggttc aagagtgatt gtatgacatt tttcctgttt gacacactaa aatctgtgta
      721 aaattagatt atttgcacag aataagaata tattcattct ttgggagttt tttatgaaaa
      781 gggcatttgt gtatcttgtt ttcagtattt ttgtttgtgt tgcatacttt cagaataacg
      841 catctgaaag tgttgttgat tatttcctag agattgcatt ggaatgcgag tacggagatg
      901 gcagcggggt tataaaaaag tggaaaatgc ctattaatat atacgttatg ggtaatttta
      961 caagggagga ttacgaggta ataaaagggc atgttgatta tctgaattcc attgaaggta
     1021 ttcctcgtgt caagctggta cgtgacatga atgaggcaaa tgtatatgta aactttataa
     1081 cccaatggga gatgtctcag aaggtttgtg ttgatgagat tgtgtgggga tttgttcggt
     1141 gtttttggaa tgaaagatat gagataacaa atgcggagat tttgattgtt tacgatagaa
     1201 ccaaccaatg ccagagacat catgtcatcc tggaggaaat gacgcaaatg cttggacttc
     1261 taaatgacag cgatttgcat gaggatagta ttttctacaa cggttattca gaaaatacgc
     1321 gcctgagccg tattgatgaa ctggtaattc gaatcttata caactcaggc gtcaaatcag
     1381 gaatgagggg tgatgatgca aggcctgttg ttagcaattg gcttgtttat ggttacaagt
     1441 taaagaattc atcaggttgt ccatttcgtg cttcttctta atagtttttg cacgcaatat
     1501 gatgttgttc tgtatatctt caggaaggtg gaggaagcat tccatcgctt ctctgttatt
     1561 tgcaagcaaa tccttcaggt ttgccggcat atccatcata aactatcacc tccgtcataa
     1621 tattatcacc aagttaacga taatttatac ctttgatatt agtagtgtgg gaataaatgt
     1681 gatataataa aggacgcctt aggcaataat taatgaaggg gtatgcaaat ggcaagaaaa
     1741 ctatttacat cagaatcagt aaccgaagga catccggaca aaatttgcga ccagattgct
     1801 gacaacatat tggatgcttt gttgaaagag gatccggaat ccagagttgc ctgcgaggta
     1861 acggctacaa ccgggcttat ccacattatg ggcgaaataa ctacaaaagc atttgtagat
     1921 tacgaaaaga tagcaagaaa cacattgcgt aatattggat acaccagttc agaaattggt
     1981 tttgatgcag atacctgtgc agttattaat actgttaatg cccagtctga agatattgca
     2041 atgggggtgg acagatctta tgagtcgaag gtggaaggtg cggaagtgga gcacggcgca
     2101 ggcgaccagg gcttgatgtt tggctttgca tgcaacgaaa caccggaact tatgccgctt
     2161 tcccttgttc ttgctcataa gttgtgcaag agacttgctc aagttcgcaa ggaaggtatc
     2221 ctgccatacc ttagacctga tggcaaagcc caggttactg ttgagtatga cgaaaatgac
     2281 aaaccattca gggtggacac cgtggttgtt tcttcacagc atgcagaaga tgtggacaac
     2341 gaaacaatca ggaaggatat tataaacgaa gttgtgttga a
//
LOCUS       DAONVY010000377         2378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_30190_length_2378_cov_3.838140, whole genome shotgun sequence.
ACCESSION   DAONVY010000377 DAONVY010000000
VERSION     DAONVY010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30190_length_2378_cov_3.838140"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLI11_09125"
     CDS             <1..585
                     /locus_tag="PLI11_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HPZ53068.1"
                     /translation="GSMIALSYETDLIAHHNIVPYLEYIYEYIKATENVAFEIRTKSA
                     NTLILSKYMPLDNLILTYTLTPQKIIDSYERFTPNLDARIKAVNHALDLGFKVRICFD
                     PIFADEDYEEFFQHVFEKIDGKRINDIGYGFFRMPKEQYLKAFKISDSIVYAKDYVEE
                     DGVVTFPEKMITEATKIHMDIIEYFVDKEKIYIL"
     gene            582..1280
                     /locus_tag="PLI11_09130"
     CDS             582..1280
                     /locus_tag="PLI11_09130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPZ53069.1"
                     /translation="MKRAIVTGSSKGIGKSIVKRLLSLGYRVYGISRTVTFDDENFIP
                     VICDILDTKSLFEVVDEIRKKEEGLDLLVNNAGIGVFGPHEQLDAKGIQEMVRLNLEV
                     PLLLTNRLLRDLVKSKGTVIFISSVTAKKTSPHGCAYAATKAGVSKFAESLFEEVRKK
                     GVKVTVIHPDMTMTNFYDESDFTCDDDESAYILPQQVADCVEFVLKMESNLAVNDITL
                     RPQINRIKRRKVIE"
     gene            1280..1981
                     /locus_tag="PLI11_09135"
     CDS             1280..1981
                     /locus_tag="PLI11_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53070.1"
                     /translation="MKIHFLGTGAADWDINNPSGDINFRRFSSVLVDDCLLIDPGPCI
                     FEFAETFKHPKLFALLKDVICTHKHADHFNQDNLNRLLGESANARFHEIDAFAKLETD
                     NHVIYSYPANHGTAVSPKNYVVESKVSGKRFFYGLDGAWILYDVYKALLEFGHLDLMV
                     FDATIGNIEGDYRIFEHNNLTMIKEMSKTFRKICSNFIISHMARTLHASHEELEKDMG
                     KYGIMVAHDNMIINI"
     gene            2079..>2378
                     /locus_tag="PLI11_09140"
     CDS             2079..>2378
                     /locus_tag="PLI11_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPZ53071.1"
                     /translation="MVKRQIIKIDEDKCNGCGLCVDACHEGAIQLIDNKAKLIRDDYC
                     DGLGNCLPVCPTGAISFEVREALPFDEEAVMAAQKAKEEDLPCGCPGSMARAIKPT"
BASE COUNT          790 a          370 c          525 g          693 t
ORIGIN      
        1 ggcagcatga ttgccctttc ctatgaaacg gatttgattg cacaccacaa catagttcca
       61 taccttgaat atatttatga atatataaag gcgacggaaa atgttgcttt tgaaataagg
      121 acaaaaagtg caaatacatt aattttgtcg aagtacatgc cactagacaa tctgattctt
      181 acatatacgc tgacacctca aaagattata gattcttatg aaagatttac cccaaatctg
      241 gatgcgagaa taaaggcggt aaatcatgcg cttgatttgg gctttaaggt aagaatttgt
      301 tttgacccta tatttgcaga tgaggattat gaagagttct ttcagcatgt atttgaaaaa
      361 attgatggaa agagaataaa cgatatagga tatggttttt ttagaatgcc aaaggagcag
      421 tatctaaaag cgttcaaaat cagcgacagt atcgtgtatg caaaggatta tgttgaagag
      481 gatggagtgg ttacttttcc tgaaaaaatg ataactgaag caacgaaaat acatatggat
      541 ataattgaat attttgtgga caaggagaag atatatattc tatgaaaaga gcgattgtta
      601 ccggaagttc caaagggatc ggaaagagta ttgttaaaag attgctctct ctggggtacc
      661 gagtatatgg tatttcaaga actgttacct ttgacgatga aaatttcata cctgttattt
      721 gcgatattct tgatacaaaa agtctttttg aggtagttga tgagataagg aagaaggaag
      781 aaggtttgga tctgcttgta aacaatgcag gcatcggagt ttttggacct cacgagcagt
      841 tggatgcaaa aggcatacag gaaatggtca gacttaatct tgaagtgcct cttttgttga
      901 ccaatagact tttgagggac ctcgtaaagt caaaagggac tgtcatattt atatcttctg
      961 taacagcaaa gaaaaccagt cctcacggtt gtgcatatgc ggccaccaag gcgggtgtaa
     1021 gcaagtttgc tgaaagcttg ttcgaggagg tcaggaaaaa gggagtaaag gttactgtta
     1081 tacatcctga catgacaatg acaaactttt atgatgaatc agactttaca tgcgacgatg
     1141 atgaaagtgc atacatttta cctcagcagg tggcggactg cgttgagttt gtacttaaaa
     1201 tggaatccaa tctggctgtt aatgacatta ctttaagacc acaaataaat agaattaaaa
     1261 gaaggaaggt aatagaataa tgaaaattca ttttttagga acaggtgccg cagattggga
     1321 tataaacaac ccaagcggtg acatcaattt caggcgtttt tcaagcgttc ttgtcgacga
     1381 ttgtctactt atcgacccgg gtccatgcat ttttgagttt gcagaaacct ttaaacatcc
     1441 caaacttttt gcccttctaa aggatgtaat ctgcacacac aagcatgctg accattttaa
     1501 tcaagataat cttaatagac tgcttggtga gtctgctaat gcaagattcc atgaaattga
     1561 tgcatttgcc aaactcgaga ctgataatca tgtaatatat tcataccctg caaatcatgg
     1621 aacagcggta tccccaaaaa attatgtagt ggaaagcaag gtttcaggaa agagattttt
     1681 ctatggactt gatggtgcgt ggattttata cgatgtgtac aaggcgcttc ttgagtttgg
     1741 acatctggat ttgatggtgt ttgatgctac aattggaaat attgaaggag attacagaat
     1801 ctttgaacac aacaatctga caatgataaa agaaatgagt aaaacattca ggaagatttg
     1861 ctcgaatttc ataatatctc atatggcaag aaccctgcat gcatcccatg aagagctgga
     1921 aaaggatatg gggaaatacg gaattatggt tgctcatgac aatatgataa taaatatttg
     1981 aattacgttg catttgcaac ggaattctgt ttttttatca tgtataattc aaccatgaag
     2041 ttggaaaact ttaaaatttg tgattaaagg tgagagttat ggtaaaaaga caaattatta
     2101 agattgatga agataaatgt aacggatgcg gactttgcgt ggatgcatgc catgaaggtg
     2161 caattcaact tatcgacaac aaggcaaaac taataaggga tgactattgc gatggccttg
     2221 gtaactgtct gcctgtttgt ccaactggag ccatttcttt tgaggtaagg gaagcacttc
     2281 cttttgacga agaagctgtt atggcagccc aaaaggctaa agaggaagac ctgccttgtg
     2341 gttgtcctgg ctcaatggca agagctatta aaccaacc
//
LOCUS       DAONVY010000378         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_30540_length_2358_cov_3.659574, whole genome shotgun sequence.
ACCESSION   DAONVY010000378 DAONVY010000000
VERSION     DAONVY010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30540_length_2358_cov_3.659574"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLI11_09145"
     CDS             complement(<1..665)
                     /locus_tag="PLI11_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53072.1"
                     /translation="MKRKVLILLLILIIVLSSCVGGGSDPTPEPTTEPTPIPAMNFEV
                     TNIKNIDIAKSGVIPDYIDMNQAQAWDCTGIDPNGIIYFGITVVRRRDEFKGKEDFAV
                     LSYNPDTEELKFHGTFMNAAKEAGTLYDDEQLPKGHTQFIYYNGKMYMGSQNFHDAGK
                     FDTKTTIPDNKPMDKMHGGHLFSLDVETEELKDETAHLPNGVLIEHEGILQIRLIPGT
                     STLV"
     gene            complement(688..1560)
                     /locus_tag="PLI11_09150"
     CDS             complement(688..1560)
                     /locus_tag="PLI11_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ53073.1"
                     /translation="MISSKYDEISFANAMFPIKVYIKSKVPMNYVQLTLTWHEQLEII
                     RFRKGGAIVQIGNHRYVAEDNDIFVFNPCEPHSLSSHAGFPEFDCIMIDKQIYSSTER
                     DICEHKYVKPMENHQIWFDNHIKNSAELLELIEQLLNEIINNEFAYELVVKGAIHNML
                     AHLFRNHINTIKSNSELKRSMLSYERLNPVFQYIENNYASEIKIENLASLTYLSTSHF
                     SRLFKQATGKTATQYINEFRISKAEVLLKTTDLSVSDVAERVGFISSSYFAKWLKTLR
                     NTTPTELRKMHAKT"
     gene            1902..>2358
                     /locus_tag="PLI11_09155"
     CDS             1902..>2358
                     /locus_tag="PLI11_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53074.1"
                     /translation="MIKKIYEETEDTPVLLGVWVPPLYRLTETDEDADMRYKELKEVG
                     VNMTYTHHPVHEVYNVNQLLRSLDAAHKHDIKVIVILRPGQLGISMVEKTKDHPAVMG
                     YNLVDEPGFGSFSQLANLRDKLKEMVDEDKIIMCNLFPNYAPDSFLGTSP"
BASE COUNT          631 a          447 c          494 g          786 t
ORIGIN      
        1 accagtgtgg aagtgccggg tatgaggcga atttgcagga ttccttcatg ttcaatcaaa
       61 acaccatttg gaagatgagc ggtttcatcc tttaattctt ctgtttcaac atccagtgaa
      121 aagagatggc cgccatgcat tttgtccatg ggtttgttgt ccggtatggt ggtttttgtg
      181 tcgaacttgc ctgcatcgtg gaagttctgc gagcccatgt acatctttcc gttatagtaa
      241 atgaactggg tgtgcccctt tggcaattgt tcatcatcgt ataacgtgcc ggcttctttt
      301 gcggcattca tgaaggtacc gtggaatttg agttcttccg tgtcgggatt gtacgacaat
      361 acagcaaagt cttccttgcc tttgaattca tcccttctgc ggacaacagt gattccaaaa
      421 tagatgatgc cgtttggatc gatgcccgta cagtcccaag cctgggcctg gttcatgtca
      481 atgtagtcgg gtataacacc ggattttgca atgtctatgt ttttgatgtt tgtaacctca
      541 aaattcattg caggaatcgg tgtcggctct gtcgtgggct cgggtgttgg atctgaacct
      601 ccgcctacgc atgacgaaag aacaataatt aatattagca atagaattaa aacttttctt
      661 ttcatatact cacctttaca tttattttta tgtttttgca tgcatttttc taagctctgt
      721 gggggtggtg tttctcaagg tttttaacca tttggcaaaa tagctgctgc ttatgaaacc
      781 aactctttca gccacgtctg aaacactcaa atctgttgtt ttcaaaagaa cttcggcttt
      841 cgatattctg aactcgttta tgtactgggt cgcagttttt cctgtagcct gtttaaacag
      901 ccggctgaaa tgggaagttg aaagatatgt gagagatgca agattctcaa tctttatttc
      961 actcgcatag ttgttttcaa tatattgaaa gacgggattg agtctttcgt aagagagcat
     1021 gctccttttc agctcgctgt tagactttat ggtgtttatg tggttcctaa aaaggtgtgc
     1081 cagcatgttg tgaatcgcac cttttaccac cagttcatac gcaaactcgt tgttgataat
     1141 ctcatttaat agttgctcta taagctcaag caattctgca ctgttcttga tgtgattgtc
     1201 aaaccaaatc tgatggtttt ccataggttt tacatatttg tgctcgcata tgtctcgttc
     1261 tgtggaggaa taaatctgct tgtctatcat aatgcagtcg aactcaggaa accccgcatg
     1321 ggaactcaat gaatgcggct cacaagggtt aaagacaaaa atgtcgttgt cttcggccac
     1381 atatctatgg ttgcctatct gtacaatagc gcctcctttt ctgaatctga ttatttcaag
     1441 ctgctcatgc catgtaagcg tcagctgtac atagttcatg ggtaccttgg attttatgta
     1501 cacttttatt ggaaacatgg catttgcaaa cgatatttcg tcatatttag aagaaatcat
     1561 cgtgttcctc ctcaatgttt gtgtatttat tttatcacaa agtttgcaat atgtcttaat
     1621 aaatattata ttttgaagac atattcaagt ctttacattg acacagtttc ctaactttgt
     1681 tctttatatt ttaaatgata gatttgtatt acattttgac ataaaaatgt tattctgcac
     1741 cttcccttgc ttatatactt gaataaagga catgtaagaa aaagtgaatg gaggattttt
     1801 taatggcatt gaaacttaag aggattattt caattttgct tgttttgttg tgcgtgttaa
     1861 atcttttttc gtgtcaagac aataccaagg aggaagagaa aatgattaaa aaaatatatg
     1921 aagagacaga agatactccc gttctattgg gggtctgggt tccgcctttg tacagactga
     1981 cagaaactga cgaggatgcc gacatgaggt acaaggagct taaagaagtg ggcgttaaca
     2041 tgacatatac ccatcatccc gtgcatgagg tatacaatgt caaccagctt ttgagatctt
     2101 tggacgcagc ccacaagcac gacatcaagg tcattgtcat attaagacct ggacagcttg
     2161 gtataagcat ggtggaaaag accaaagacc atcctgctgt tatgggatac aacctggtgg
     2221 atgagcctgg atttgggtcc ttttcacaac ttgcaaatct tagagacaag ttaaaggaaa
     2281 tggttgatga ggataagatt ataatgtgta atctttttcc caactacgct cctgattctt
     2341 tcctaggaac atctccca
//
LOCUS       DAONVY010000379         2344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_30780_length_2344_cov_2.420271, whole genome shotgun sequence.
ACCESSION   DAONVY010000379 DAONVY010000000
VERSION     DAONVY010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30780_length_2344_cov_2.420271"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLI11_09160"
     CDS             <1..835
                     /locus_tag="PLI11_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53075.1"
                     /translation="EQFMYPKKGPGQLWEQMASEIVKMGGEIRLNSKVVGVDTKENRV
                     ISVRTDKETIKGDLFFSSMPIKDLAYALDECPDKVRDVAVYLPYRDFITVGLLLRKLK
                     IANTTRIRTINNIVPDCWIYIQEPEVKLGRLQVFNNWSPYMVSNPDNVFIGLEYFCCE
                     NDELWNMSDKEFADFAARELEEIGIIDMGDVLDSITIKVKKAYPAYFGTYSEFNIVRE
                     YLDSFENLFCIGRNGQHRYNNMDHSMLTAMVAVENIKNGVRDKSNIWNVNTEAEYHEE
                     K"
     gene            complement(836..1102)
                     /locus_tag="PLI11_09165"
     CDS             complement(836..1102)
                     /locus_tag="PLI11_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53076.1"
                     /translation="MAGNFIPQTSAKNLEVLDLPDCVHLTDNFKCEILKVNECMGREC
                     SFMLNQKQKSKSYNLWKKKMNELSESKQKDIAHTYFNGKMPWKS"
     gene            complement(1224..>2344)
                     /locus_tag="PLI11_09170"
     CDS             complement(1224..>2344)
                     /locus_tag="PLI11_09170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ53077.1"
                     /translation="MRFETNKLYEDKHTQSFSINVLDGDRCVSTARIGYVDSVFAGKY
                     IKAMTVGGVATEPEYRRKGFVRKMFQMAFNDAISKGACASFLHPFSFSYYRKFGYEKV
                     ADQLIVEFPMNKLSHIPRESNLHLLKGSDRLEDVISIYNEFALKRNIMFYRHNGRHFF
                     TNPDRFKGSTYIWYDKDGKPSSYISLSVENYYHVNHMRSINLNVYEMAFTSKESLLKL
                     FGFMRMFEGENDTVKIHNYAMSPEIDLILRHYIHTTYKRYPDLMARILDVKTILESNT
                     YPQGKGHFRVNITDDLEYTKGVWEVEFENGVGVAKRVDKDKYDVSLSMPAFTQIVYGY
                     DKYNADVLAYMDGVEIFNEKSDFFHVFPKRYNGLFEHF"
BASE COUNT          705 a          417 c          467 g          755 t
ORIGIN      
        1 tgagcagttt atgtatccca agaaaggtcc ggggcaacta tgggaacaga tggcttcgga
       61 aattgttaaa atgggaggag agattagact caactcaaaa gttgttggag ttgacacaaa
      121 agagaacaga gttatatcag taagaacaga taaggaaaca attaaaggag acttgttttt
      181 ctcttccatg ccaataaaag accttgcata tgcattggat gaatgtcctg acaaggtaag
      241 ggatgtagct gtatatcttc cttaccgcga ctttataact gttggtcttt tgcttaggaa
      301 gttgaaaatt gccaacacaa ccaggattag aacaataaac aacattgtgc ctgactgttg
      361 gatttacata caggagccag aggtaaagct gggaagactt caggtattca acaactggtc
      421 gccatatatg gtaagcaatc ctgataatgt atttattggt cttgaatatt tctgctgcga
      481 gaatgatgaa ctgtggaaca tgagtgataa agaattcgca gactttgcag caagagaact
      541 ggaggaaata gggataatag atatggggga tgttcttgat tcaattacca taaaggtaaa
      601 gaaagcctat cctgcatatt tcggcaccta ttcggagttt aatattgtca gagaatatct
      661 ggattccttt gaaaatctct tctgcatagg acgaaacggg cagcataggt acaacaatat
      721 ggaccattct atgcttactg ccatggtggc tgtagagaat ataaagaatg gggtaaggga
      781 caagtcaaat atatggaacg taaataccga ggcagaatac catgaggaaa agtgatcagc
      841 ttttccatgg cattttgcca ttgaaatatg tatgtgcgat gtctttttgc ttgctttcgc
      901 ttagttcgtt catttttttc ttccaaaggt tgtatgattt gcttttttgc ttctggttga
      961 gcataaagga gcattctctt cccatacatt catttacttt taatatttca catttaaagt
     1021 tgtcggtaag atgcacacaa tcgggcaaat caaggacttc caaattttta gcgcttgttt
     1081 gaggtataaa gtttccagcc atctctatct ccttaaaaaa ataaattgca taaagaacat
     1141 gaacaaacta tattatatca tgtttgaaaa ttttatgtaa taaaatatgt taattttttt
     1201 taaaattgtt cgattctatt tatttaaaaa tgttcgaaca aaccgttgta cctttttgga
     1261 aagacgtgaa agaaatctga tttttcgttg aatatttcaa caccatccat gtacgccaga
     1321 acatcagcgt tatatttatc ataaccgtat actatctggg tgaaagcagg catcgaaagg
     1381 gatacgtcgt atttatcctt gtctacacgt tttgcaacac cgactccgtt ttcaaactcc
     1441 acctcccaga caccctttgt gtactcaagg tcatcggtga tgttgactct gaaatgcccc
     1501 ttaccttgag ggtatgtgtt tgattcaaga atggttttga catcgagaat tcttgccata
     1561 agatccggat atcttttata cgttgtatgg atgtaatgtc tgagaatcaa gtcaatttca
     1621 ggagacatgg catagttgtg tattttcaca gtgtcattct ctccttcaaa cattctcatg
     1681 aacccaaaga gttttagcaa ggattccttt gaggtaaagg ccatttcata cacatttaaa
     1741 ttgatggatc tcatatgatt tacatggtaa tagttttcaa cggaaagaga gatgtatgat
     1801 gaaggctttc cgtctttgtc ataccagatg tatgtggaac ctttaaatct gtcgggattg
     1861 gtaaagaaat gtctgccgtt atgtctgtag aacatgatgt ttctttttaa tgcaaactcg
     1921 ttatatatac ttataacatc ctccagtctg tcactgcctt ttaaaaggtg caaattactt
     1981 tcgcggggga tatgggacag tttgttcatg ggaaactcaa caatcaactg atccgctact
     2041 ttttcataac caaattttct gtaatatgaa aaggaaaagg gatgcaggaa acttgcgcaa
     2101 gcccccttgc ttattgcgtc gttaaatgcc atttgaaaca ttttccttac aaagcctttt
     2161 cttctgtact caggttctgt ggcaactcca cctactgtca tggcttttat gtattttccg
     2221 gcaaacacgc tgtcaacata acctatcctt gctgtggaca cgcatctatc gccatccaga
     2281 acatttattg aaaagctttg tgtatgcttg tcttcataaa gcttgtttgt ttcaaatctc
     2341 atgg
//
LOCUS       DAONVY010000380         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_30803_length_2342_cov_3.915173, whole genome shotgun sequence.
ACCESSION   DAONVY010000380 DAONVY010000000
VERSION     DAONVY010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30803_length_2342_cov_3.915173"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="PLI11_09175"
     CDS             <1..132
                     /locus_tag="PLI11_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HPZ53078.1"
                     /translation="DDREWTFWQYSDRGRLNGYDGVERFIDLNVFNGTREEFAYFCR"
     gene            complement(389..1756)
                     /locus_tag="PLI11_09180"
     CDS             complement(389..1756)
                     /locus_tag="PLI11_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPZ53079.1"
                     /translation="MKRNPKQRLKHSLIISAIALMLLIALTSCFDANEIDDMIYIVCV
                     GVDRGVADKWRITLQYENLKGSSPQSQSGSSSSSGDDQNQQGKEEDSLKEKVEDQGNI
                     SYVTIDAPSFFAGIDMLNTSISRKISFMHLQFIVFSQELAKSEELSTFLAPIMRFREI
                     RETVHIYITKNKAMDIIKANTPIIGSALAKDHLIWTRESESTGFFPHSILNNFYNSLK
                     SNRRFPITAIISLNEMKNFIEEGKPYKGELKSGGEYLSGKLPRLGLNQLEIWGVALFD
                     GDIMVGELNGKETRVLLILRGEFERAFVNIEDPANPDYAIPLDVTVREQPKIKVNIKN
                     GKPVITVDIKLDADILAIQSHVYYENPVNKKVLEEACANRMLDEVEKLIVKCRDLGVD
                     PFKFGEYASRQFLTIQELEEYNWNEKFKDATFIVNLSVIVRRTGTMMKSSKIISPEGQ
                     PKEVE"
     gene            complement(1722..>2342)
                     /locus_tag="PLI11_09185"
     CDS             complement(1722..>2342)
                     /locus_tag="PLI11_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HPZ53080.1"
                     /translation="INNMFPIFGYGLDKTILHGVIRSSAYGEVILLAVYASNLQGTRF
                     IKHEGYYSLLISTIVISLILFTFTMCYPYYRLQEMVAPMYNLTTLIQFGRFFTRVESI
                     FLFIWIVGSLIGISLIFYSSIWLYCKIFNINDKRPIILAGCVILFLISLLQKDIISVI
                     TGNVSLIRYYGFIPYFIIPLLALIVAAIRRKKGDSNETESEAKAQT"
BASE COUNT          655 a          479 c          440 g          768 t
ORIGIN      
        1 gatgacaggg aatggacgtt ttggcagtat tccgaccgcg gacgactgaa tggatacgac
       61 ggggtggaga ggtttattga tttgaatgtt tttaacggta cccgtgaaga atttgcatac
      121 ttctgcagat agtgcgagct gacaatcaaa gcatctccgt aagaaaggtg tacggagatg
      181 ctttatcatt gtgttttggt gctgtttgct cggtacaatt aattaaatat cagccatata
      241 caaaaagact tgtacaacaa ttatggctat gttgataata aatgtaccga gtgcagccat
      301 tttgtttttg tttttccagt tatgaatagc aaaagttaca aggtggtagt gcaagagaaa
      361 caacagtagg ataaaaatat atttcatatc actccacctc ttttggttgt ccctcgggac
      421 tgattatctt gctgctcttc atcatggttc cggttctgcg gacaattacg gacaagttga
      481 caataaaggt tgcatctttg aatttttcat tccagttata ctcctcaagc tcctgtatgg
      541 ttaaaaactg ccttgaggca tattctccaa atttgaaggg atctactccc aggtctctgc
      601 acttaacaat caacttttca acctcatcca acatcctgtt tgcgcatgct tcttctagaa
      661 cctttttatt aacaggattc tcgtagtaca catgactctg tatcgccaga atatctgcat
      721 ccagctttat atcaacagtt attaccggtt ttccattttt tatattaact tttatctttg
      781 gttgttctct gacggttaca tcaagaggga tggcatagtc cgggtttgca ggatcctcaa
      841 tattaacaaa cgctctttca aactcgcctc ttagtatcag caaaacccga gtttcctttc
      901 cgtttagctc tccaaccatt atgtcgccat caaaaagagc aacaccccat atctcaagct
      961 ggttgagtcc caatctcggc agcttacctg acagatattc tcctcctgat ttcagttctc
     1021 ctttgtatgg tttaccttct tcaatgaagt ttttcatttc attaagtgat attattgccg
     1081 taattggaaa tcttctgttt gatttcaggc tgttatagaa attgttaaga attgaatggg
     1141 gaaaaaaccc tgtactttcg ctttctcttg tccatatcaa atggtctttt gccagtgcgg
     1201 aacctatgat aggtgtgtta gcttttataa tgtccatggc tttgtttttg gttatataga
     1261 tatgaacagt ctcgcgtatt tccctaaatc tcataatcgg tgccaaaaaa gtagacaact
     1321 cttcgctttt ggccagctct tgcgagaaca ctatgaactg cagatgcata aaacttatct
     1381 tccttgaaat gcttgtgttt agcatgtcta tccccgcaaa gaaggatgga gcatctattg
     1441 tcacatagga tatgttcccc tgatcttcaa ctttttcttt tagagaatct tcttctttcc
     1501 cttgctgatt ctggtcatca cccgaggagc tgctggaacc actttgagac tgaggagaac
     1561 ttcccttcaa gttttcatat tgaagtgtta ttctccactt gtcagcaaca cctctgtcca
     1621 ctccaacaca gacaatatat atcatgtcgt cgatttcatt tgcgtcaaaa catgaggtca
     1681 gagcaattaa tagcattaat gcaatagctg agataattag actatgtttg agcctttgct
     1741 tcggattccg tttcattgct atcccccttt ttccgtctga ttgccgcaac tatcaatgca
     1801 agcagcggaa tgatgaaata tggtatgaaa ccatagtatc taattaagct tacatttcct
     1861 gttataacgg atatgatgtc cttctgcagg agtgatataa ggaacaggat gacacagcct
     1921 gccagaatta tgggtctttt atcatttatg ttgaatattt tgcagtacag ccagatggaa
     1981 gagtaaaata ttagcgatat acctatcaaa gaccctacta tccagataaa cagaaaaatt
     2041 gattcaactc ttgtaaaaaa tctgccgaac tgtattagcg tggtaaggtt gtacatcgga
     2101 gcaaccatct cctgcaacct gtaatacgga taacacattg taaaagtgaa aagtatcaga
     2161 gatattacaa tagttgatat cagcaagcta tagtatcctt catgtttgat aaatctggta
     2221 ccctgaagat tgcttgcata tacagcaaga agtatcactt ctccataggc tgagcttctt
     2281 attactccgt gcaatatggt tttatccagc ccatagccaa agatgggaaa catgttgttg
     2341 at
//
LOCUS       DAONVY010000381         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_30910_length_2336_cov_2.386234, whole genome shotgun sequence.
ACCESSION   DAONVY010000381 DAONVY010000000
VERSION     DAONVY010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30910_length_2336_cov_2.386234"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..825)
                     /locus_tag="PLI11_09190"
     CDS             complement(76..825)
                     /locus_tag="PLI11_09190"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPZ53081.1"
                     /translation="MSENILQEDKRMNKRIRRAKVVRTSAITFVCFVLGILIAMQYKN
                     IREIKANEERSYNSLYEYQKELIKLQEEVDKLTQEKAELQKKVDLFEHGSYQQQIEHL
                     EKELKNVKLFAGLTKVKGSGIEIIIELTDPSTLENIAAALLMLINELKASTAQAIAIN
                     GVRIGAMSEVRVVATSLVIDGQVYGQPIRIAAIGDQNNLLGILSTTRALVDHIGKFGA
                     VTITPRDNLILDAYDFEKVERYTSKLTPVEE"
     gene            complement(838..1569)
                     /locus_tag="PLI11_09195"
     CDS             complement(838..1569)
                     /locus_tag="PLI11_09195"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPZ53082.1"
                     /translation="MFSFFKQKLTVKEKIIVMACMLLLGAVLSMQARAVYENRQQEEA
                     GKQKELNEYLEKINELKEEVQSLTSDITTLKGRYAIYLKNLEQNEPVFYNRLKHLNDS
                     IQEFKLYAGLTDVKGPGIEIRMDDGEYDMIVHDNFLYELVNELRAAGAQAIEVNGKRV
                     VTSTEFLCIGPSIRVNDEGVFAPYVIKAIGEPSVLERRVNESFIYGDIESRNIRIDIR
                     RYDEIYIRKYNDDYYKKIRHLAETE"
     gene            1696..>2336
                     /locus_tag="PLI11_09200"
     CDS             1696..>2336
                     /locus_tag="PLI11_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPZ53083.1"
                     /translation="MEAKSGYVSISTENIFPIIKKWLYSEKDIFIREIVSNACDAINK
                     MKKLVSIGEVDLPNEEMNWKIEVWLDKKKKTITVYDNGIGMTEEEVLKYINQIAFSGV
                     KDFVEKYEGKTDESQIIGHFGLGFYSSFMVSKKVEIDTMSWAEGAEPIHWESNTGMDY
                     SISPSKKINKRGTYVTMHIADDSKEFLDKFKLRETLKKYFSFLPVDLCLIDAN"
BASE COUNT          647 a          516 c          435 g          738 t
ORIGIN      
        1 aaaaaacctc ccttttgcat acatgtctct gtaaagatat gcaaaaagga ggcaaattat
       61 tctatgtccc acattctatt cttccactgg cgtcagcttg cttgtgtacc tttcaacttt
      121 ttcaaagtca taggcatcca gtatgaggtt gtctctagga gttattgtaa ctgctccgaa
      181 tttcccgata tgatctacca gtgctcttgt ggtagagagt atgccaagaa ggttgttctg
      241 gtccccgatg gctgctatgc ggatgggttg accgtaaacc tgaccgtcta tgacaaggga
      301 tgtggcaacc acccttacct cgctcatagc cccgattctt acaccattta tggctatggc
      361 ttgagcagtg gaggccttaa gctcatttat gagcatgagc agtgctgccg caatattttc
      421 caatgtagaa ggatccgtaa gttctataat aatttctatt cctgaccctt ttacctttgt
      481 aagcccggca aaaagcttga cattcttaag ctctttttcc aggtgctcaa tctgctgctg
      541 gtagcttccg tgctcgaaca ggtcaacctt tttttgcaat tccgcttttt cctgagtcag
      601 tttgtccact tcctcctgca gctttataag ctccttctgg tattcataca aggagttgta
      661 ggacctttcc tcattcgcct tgatttccct tatgttcttg tactgcattg caatcaggat
      721 tccaagaacg aagcatacaa aagtgatggc agatgttctt accacttttg ctcttcttat
      781 tctcttgttc attcttttat cttcctgcaa aatgttttca ctcattttaa tccctcttta
      841 ctctgtttct gccaggtgcc tgattttctt gtaataatcg tcattatact tgcgaatata
      901 tatttcgtca tagcgcctta tgtcgattct aatatttcta gactcaatat ctccgtatat
      961 gaagctttca tttactcttc tttccagcac cgaaggctcc cctattgcct tgattacata
     1021 cggagcaaac actccttcgt cattcaccct tatggacgga ccaatacaca gaaactccgt
     1081 actggtaacc actcttttgc cgtttacttc tattgcctgg gcccctgcag ctcttagctc
     1141 atttacaagt tcatacagaa agttatcgtg aactatcata tcgtattcac catcatccat
     1201 tcttatctct ataccaggac cctttacatc cgtaaggccg gcatacagct tgaattcctg
     1261 gatactgtca ttcagatgct tcaatctgtt gtaaaataca ggttcattct gctccaggtt
     1321 tttaagataa atagcatacc tgccctttaa tgtggtaatg tcacttgtaa gggattgtac
     1381 ttcttccttc aactcgttta tcttttcaag atattcgtta agttccttct gctttcccgc
     1441 ttcttcctgc tgcctgtttt catatactgc ccttgcctgc atggaaagaa ctgctccaag
     1501 aagcagcatg caagccatga ctattatctt ttccttaaca gttaattttt gtttaaaaaa
     1561 agagaacata ataagcaacc ccttctagaa cacaattata tcacaacggg ctgtaaacga
     1621 aaaggagcaa tttgattttt atctgctgtt ggtatataat tcccttttga aagtatatca
     1681 gcaaggagga taagaatgga agcaaagagt ggttatgtat caataagcac tgagaatata
     1741 tttccgatta ttaagaagtg gctttattcg gaaaaggaca ttttcatcag ggaaatcgtt
     1801 tcaaacgcat gtgacgcaat aaacaaaatg aagaagctgg tttccatagg tgaagtggat
     1861 ctacctaatg aggaaatgaa ctggaaaata gaggtctggc tggacaagaa aaagaaaacc
     1921 attacggttt atgacaacgg catcggcatg accgaagaag aggtcctaaa gtatatcaac
     1981 cagattgctt tttccggtgt aaaagatttt gttgagaaat atgaagggaa aactgacgaa
     2041 tcacaaatca taggacattt cggtcttggt ttctactctt ccttcatggt gtccaaaaag
     2101 gttgaaatag acaccatgtc ctgggcagaa ggcgcagaac caatccattg ggaaagcaat
     2161 acaggaatgg actattcaat aagtccgtcc aagaaaatca acaagcgcgg aacatacgta
     2221 acaatgcaca tagcagacga cagcaaggag tttctcgata aattcaaact gagggaaaca
     2281 ctgaaaaaat atttctcttt cctgcctgtc gacctgtgtt tgattgatgc taacga
//
LOCUS       DAONVY010000382         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_30949_length_2333_cov_3.332748, whole genome shotgun sequence.
ACCESSION   DAONVY010000382 DAONVY010000000
VERSION     DAONVY010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30949_length_2333_cov_3.332748"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLI11_09205"
     CDS             <1..261
                     /locus_tag="PLI11_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53084.1"
                     /translation="VFKMKIADIKHMEEMQRNKWLYAISLIPGTYVRQISRIVGLSYS
                     FIRKCLKSFEEFINDPVRLDRLKKNVINKLINHKDITSCASL"
     gene            complement(456..1301)
                     /locus_tag="PLI11_09210"
     CDS             complement(456..1301)
                     /locus_tag="PLI11_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPZ53085.1"
                     /translation="MLPFKVDLRDKVVVITGGGGVLCSAFAEALAECSAKIAILDINA
                     DAAKSIAERIIEKGGFAQGFEADCLNKESLEKAREEIIKAMGACDILINGAGGNSPKG
                     TTDNEQFMPGDLEKEIKTFFDLDPAGISFVFDLNILSAVLTTQVFAKDMVKNERGNII
                     NISSMNAFRPLTKIPAYSAAKAGVSNLTQWMSVHFAPSNIRVNAIAPGFFVTHQNRNL
                     LFDESGNPTARSKKILSHTPMNRFGDPSDLIGTLLWLANDEASGFVTGTVIPVDGGFA
                     AYSGV"
     gene            complement(1313..>2333)
                     /locus_tag="PLI11_09215"
     CDS             complement(1313..>2333)
                     /locus_tag="PLI11_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ53086.1"
                     /translation="AVFTDPEEMIKSGTVDAVVIATPHYDHPVLAIKAFENGLHVVIE
                     KPAGVYTKKVREMNEAAAKSGKKFMIMFQNRTNPLYAKMHELVAAGAIGEIKRVTLLI
                     TDWYRPQSYYDSGSWRATWEGEGGGVLLNQAPHNLDLWQWITGMMPSKIRANCHMGKW
                     HDIEVEDDVTAYFEYPNGATGVFVTSTGDYPGTNYFEILGDEGKLVVTGGQLAYYKNK
                     MNEREFNRANEVSFAIPGHERVEVEIPKPEGPLGHIWMMNNFFSSILHDTPLYVPGEE
                     GIKSLMMSNAMYLSGWLDKTVELPIDEDEFLAELNKRRATSNFKAKVSKDDVVVEDIS
                     KTFMK"
BASE COUNT          633 a          538 c          418 g          744 t
ORIGIN      
        1 gtttttaaaa tgaagatagc agatataaag cacatggaag agatgcaaag aaacaaatgg
       61 ctgtacgcaa tcagtttaat tcctggtaca tatgtaaggc aaatatccag aatcgtgggt
      121 ctttcgtaca gtttcatacg taaatgctta aagagctttg aggaatttat aaacgaccct
      181 gtaagacttg atagattaaa aaagaatgta ataaacaaac ttattaatca caaggacata
      241 acaagctgcg caagcttata attattaaat tcattataaa cctgattatc ccccaatcag
      301 atttgtttcc ccctgaaaat aagtgatatt ttatacttta tacttttctg cttaaaagtg
      361 cgggtttgct ttggcatgcc ttggttttag cataaaaaaa gcccttcact ttaggaagga
      421 ctttagtttt ttatgtttat gttttgcaat catgtttata ctcctgagta tgctgcgaat
      481 ccaccgtcaa ccggaatgac tgtgcctgtt acaaagcctg aagcctcgtc gttagccagc
      541 cacagaaggg ttccaattag gtctgaaggg tcgccaaagc ggttcattgg agtatgagac
      601 aatattttct ttgatctggc tgttggatta ccgctttcat caaacagaag atttctgttc
      661 tgatgagtaa caaagaaacc aggtgctatt gcgttcactc ttatgttgct tggtgcaaag
      721 tgcacgctca tccactgagt cagattagac acacctgcct tggcagcact gtatgcaggt
      781 atcttagtca atggcctgaa agcgttcatg gatgaaatat ttataatatt tcctcgctca
      841 ttctttacca tatcttttgc aaacacctgc gttgtcagca cggcagacag aatattcagg
      901 tcgaaaacaa aactgatgcc tgctggatca agatcaaaaa atgttttaat ctctttttcc
      961 aagtctccgg gcataaactg ctcattgtct gttgtaccct tggggctgtt gccacctgcg
     1021 ccatttatca gtatatcgca agcacccata gccttgataa tttcttctct tgctttctca
     1081 agactctcct tgttaaggca gtcagcttca aatccttggg cgaaaccacc cttttcaata
     1141 attctctctg cgatactttt tgctgcatcc gcattaatgt ctaaaatggc tatttttgcg
     1201 ctacactcag ccagggcttc agcaaaagct gaacacagca cgcctccacc accggtaata
     1261 actacaacct tgtctcttaa atctacttta aaaggtaaca tttcttcact ccttatttca
     1321 tgaaggtttt ggatatgtct tcaacaacaa catcatcctt gctcactttg gccttgaagt
     1381 ttgaagttgc tctcctcttg ttaagctctg caaggaactc atcctcatca attggcagtt
     1441 ctactgtctt gtcaagccaa cctgacaaat acatcgcatt cgacatcatc aatgacttta
     1501 tgccttcttc tccgggaaca taaaggggag tatcgtgcag gatgctgctg aagaagttgt
     1561 tcatcatcca aatatggcca agaggtcctt ctggttttgg tatctctact tcaacccttt
     1621 catgaccagg tattgcaaag cttacttcat tagctctgtt gaactctctc tcattcatct
     1681 tgttcttgta gtatgcaagc tgtccacctg ttaccaccag ttttccttca tctcctaaaa
     1741 tctcgaagta attcgttcct ggatagtctc ctgtggacgt tacaaacaca ccagttgctc
     1801 catttggata ctcaaagtat gcagttacat cgtcttctac ctctatgtca tgccatttac
     1861 ccatatgaca attagctcta atttttgaag gcatcatgcc tgtaatccac tgccacagat
     1921 caaggttgtg gggtgcctgg ttaagcaaga ctccgccacc ttctccttcc catgttgcgc
     1981 gccatgaacc tgaatcataa tatgactgag gtctgtacca gtcagttatc aacaaggtca
     2041 cccttttaat ttcacctatt gcgcctgcag ctaccaattc atgcattttt gcatacagag
     2101 gatttgtcct gttttgaaac attatcataa acttcttacc ggacttggca gctgcttcat
     2161 tcatctccct gactttcttt gtataaacgc cagcaggttt ctcaattacc acatgcaatc
     2221 cgttttcaaa tgccttaata gctaatacag ggtggtcata gtgtggagtt gcaatgacta
     2281 ctgcatccac tgttccagat ttaatcattt cttcaggatc tgtgaaaacc gca
//
LOCUS       DAONVY010000383         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31080_length_2325_cov_3.179295, whole genome shotgun sequence.
ACCESSION   DAONVY010000383 DAONVY010000000
VERSION     DAONVY010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31080_length_2325_cov_3.179295"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PLI11_09220"
     CDS             <1..1501
                     /locus_tag="PLI11_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53087.1"
                     /translation="ADLKDDRYVINSYNTHAEIKAANDCTVHAAVSRFFLESKFSGLG
                     DFSPFVGQIDFTPSSSVRGMYFATHFENFYHKAPIEKIYDIIEELAFRGCNTLLVWYD
                     MHHYNSIEQPESVEMINRLKEILHYAQRIGIKAAMLMIANEAFASSPEHLRANWHVQG
                     RYKQELCGHYNVEICPSKKGGVDEILRSRRQVLEVFSDIQLSYIVYWPYDQGGCTCKD
                     CEPWGVNGFLKIYPHFCSLVKEIMPQTEIILSTWLFDHFLDGEWEELCNLLARNELDS
                     VSYIMSFFHYGDVPECVRNSGILEKVKFIDFPEISMHLCSPWGGFGANPLTRFLQQTY
                     EGTKGMFHGGFPYSEGIFEDLNKWVILSLYSGLYDNAVDAVRGYVKHEFLCEDELLVD
                     AIIRTEDFLYRQSYSDGKVMHYDIADTGSIEYVYEIITNWNELLTEEIGSSFKWRLIY
                     LRAVIDHELLHNDFIPKKSQRIQEALKELSDMYYATENTNPWVKPPLNM"
     gene            1533..2021
                     /locus_tag="PLI11_09225"
     CDS             1533..2021
                     /locus_tag="PLI11_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HPZ53088.1"
                     /translation="MTLRQAKIEDLPDIVDIYNSTIPGRMVTADTEFVTVEDKLDWFY
                     AHNSEKRPLWVVEIDGEMCGWVSLQPFYGRPAYNATAEISIYLHENFRGQGIGKKVLQ
                     KVLDECPGLGIDTVLGFIFGHNEQSIRLFTRLGFDRWGFLPGIAILDGVRRDLVILGK
                     KL"
     gene            complement(2053..>2325)
                     /locus_tag="PLI11_09230"
     CDS             complement(2053..>2325)
                     /locus_tag="PLI11_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HPZ53089.1"
                     /translation="LIATVGFETGCSRIVINKSTICEEFFDLKTRLAGEVLQKFINYN
                     IKLAIVGDFSVYSSKSLRDFIYESNKGKDIFFLPTEHEAVDKLSQI"
BASE COUNT          665 a          395 c          532 g          733 t
ORIGIN      
        1 tgccgattta aaggatgaca ggtatgttat taactcatac aacacgcatg cagagataaa
       61 agcagccaat gattgtacag tccatgcagc agtttcccgt ttctttttgg aaagcaagtt
      121 tagtggtttg ggcgactttt ctccattcgt cggtcaaatt gattttactc ccagtagttc
      181 tgttcgtgga atgtattttg caactcattt tgaaaacttt tatcacaagg cacctataga
      241 aaaaatatat gatataattg aggaacttgc ttttaggggc tgcaacactt tgcttgtctg
      301 gtatgacatg caccattata attcgattga gcagccagag tctgtggaaa tgattaacag
      361 gcttaaagaa atattgcact atgcacaaag gattggaatt aaggcggcaa tgctcatgat
      421 agccaatgaa gcttttgcgt cttcccctga acaccttcgt gccaactggc atgttcaggg
      481 cagatacaaa caggagctct gcggtcacta caatgttgag atatgtcctt ccaaaaaggg
      541 tggtgttgac gaaatcttga gaagcagaag gcaggttctt gaagtttttt cagatattca
      601 gctgtcatac attgtatact ggccttatga tcagggaggt tgtacctgta aagactgtga
      661 gccctggggt gttaacggct ttttgaaaat atatcctcat ttttgcagtc tggttaagga
      721 aattatgccg cagacggaaa ttattctgtc aacttggctt tttgaccatt ttttagacgg
      781 tgaatgggaa gagctttgca atctgcttgc aaggaatgag ttggattctg tttcatatat
      841 catgtccttt ttccactatg gtgatgttcc tgaatgtgtt aggaactcgg gaattcttga
      901 aaaagtaaaa ttcattgatt ttcctgaaat aagcatgcat ttatgttctc cctggggagg
      961 ttttggagca aatccattaa ccaggtttct tcagcaaacc tacgagggga ccaaaggaat
     1021 gtttcacggc ggtttccctt attcagaagg catctttgag gacttgaaca agtgggttat
     1081 tttgtcgtta tatagcgggc tatatgataa tgcagtggat gctgtaagag gatatgttaa
     1141 acatgagttt ttgtgtgagg atgagttatt ggtggatgcg attattcgga ctgaagactt
     1201 tctttaccgc cagagctaca gtgacggaaa ggtcatgcat tatgatatag ctgatactgg
     1261 cagtatagag tatgtatatg aaataataac aaactggaat gaattgctga ctgaagagat
     1321 cgggtcgtcg tttaaatggc ggcttatata ccttcgtgcg gttattgatc atgagctttt
     1381 acataatgac tttataccaa aaaaatctca gcgtatccag gaggcgttga aagaattatc
     1441 cgatatgtat tacgcaactg aaaataccaa tccatgggta aagccgcctt tgaacatgta
     1501 agtggtttta tctgaaagga gaattgcaag tcatgacatt aaggcaagca aaaattgaag
     1561 atttgcctga catagtcgat atatacaact caaccattcc aggcagaatg gtaactgctg
     1621 atactgaatt tgtgacagtc gaagataaac ttgactggtt ttatgcacat aattctgaaa
     1681 aaagaccgct gtgggttgtt gagattgatg gtgaaatgtg cggatgggta agtcttcaac
     1741 ccttttatgg aaggccggca tacaacgcaa ctgctgagat tagcatttat cttcatgaaa
     1801 acttcagagg gcagggaata ggtaaaaagg tattgcaaaa agttttggat gagtgcccag
     1861 gactcggaat agatactgtt cttggattta tcttcggaca caacgaacag agcattcggc
     1921 tgtttaccag actgggtttt gataggtggg gttttctgcc tggtatagcc attcttgatg
     1981 gagttagaag agacctggtc attctgggaa aaaaactttg attccattct actgctttcc
     2041 ttggacatac ctttaaattt gacttagctt gtccacagct tcatgttcgg taggcaagaa
     2101 gaatatgtct ttgcctttat tgctctcgta gatgaagtct cttaggcttt tgcttgagta
     2161 tacagaaaag tcgccaacaa ttgctagttt tatattgtaa tttatgaatt tttgtaatac
     2221 ttcgccagca agtcttgttt ttaaatcaaa gaattcttca catatggtag acttgtttat
     2281 aactatcctt gagcagcctg tttcaaaccc tacggtggct ataag
//
LOCUS       DAONVY010000384         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31142_length_2321_cov_3.538835, whole genome shotgun sequence.
ACCESSION   DAONVY010000384 DAONVY010000000
VERSION     DAONVY010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31142_length_2321_cov_3.538835"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLI11_09235"
     CDS             complement(<1..347)
                     /locus_tag="PLI11_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mucin desulfatase"
                     /protein_id="HPZ53090.1"
                     /translation="MITPNEKILSQFAFSGKHISTEPIIAGHINNTFLVTYDKAKYTL
                     QRINTNVFKDPYKLMSNIQGITKHIYNKMKSLGNDPERATLRVVETINGQPLFKDEDG
                     HFWRAYYYIDDAKA"
     gene            543..977
                     /locus_tag="PLI11_09240"
     CDS             543..977
                     /locus_tag="PLI11_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53091.1"
                     /translation="MKTRKQERKKTVLVWVTNPDACQSIVSAGRKLADDNKVELVIVS
                     IQGSIKGDWNNRASDLKKLSEAAKSADAELTVVYSDDMYEAAHDTIKRFKPMIMVTGL
                     PGNLGAGSFLEQIMNMAPGVPIYSVDMKENMKRLDTISLRIS"
     gene            1074..1841
                     /locus_tag="PLI11_09245"
     CDS             1074..1841
                     /locus_tag="PLI11_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPZ53092.1"
                     /translation="MKLIYIKHGRQFLIISLVLVIVMMLAVVSMVKDHLGRAQETLLP
                     IYGTDDDNMRISLTINCAWGADDIPEILDILDREQVKATFFVLGIWAEKNPEMLKLIH
                     DRGHEIGNHSYSHKLPSRISVKQMEEEIDKANDAIEKIVGFRPLLYRAPSGDHTEELL
                     TLTQRKGLYTIKWTVDSIDWKDSMTAQNIIDRVTGRTRSGSILLFHNDTKHTKDVLPV
                     IIKHLKNEGYDFVKVSELIYKTDYYIDHQGIQRGKRG"
     gene            1934..>2321
                     /locus_tag="PLI11_09250"
     CDS             1934..>2321
                     /locus_tag="PLI11_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="binds to single stranded DNA and may facilitate the
                     binding and interaction of other proteins to DNA; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPZ53093.1"
                     /translation="MSAELAENNVASISGIISSEIEYSHKIYGESFYNFYVVVMRLSK
                     SYDTIPVTISERIVPMEKLQKGKYVEVNGQFRSYNIADGESRLMLTLFVKDINIADAK
                     PDKPDVNQIELNGFICKQPVYRVTPFN"
BASE COUNT          751 a          384 c          522 g          664 t
ORIGIN      
        1 gctttcgcat cgtcaatata ataataggct ctccagaaat ggccatcttc gtccttgaac
       61 aaaggttgtc cattaatggt ttccacaacc cttaaagttg ctctttccgg atcattgcct
      121 aatgatttca ttttgttgta tatgtgtttg gttatccctt gaatgttgct cattagtttg
      181 tacggatcct tgaaaacatt tgtgttgatg cgctgcagcg tatactttgc cttgtcgtat
      241 gtaactagaa aagtattatt gatgtgacct gcaattattg gctctgtgct tatgtgcttg
      301 ccactaaaag caaactgact caatattttt tcgtttggag ttatcatgtt atcccttcct
      361 taagcttgtt agataaaata atattacata aaaatttata agtcaataat ttcgattaat
      421 gatttttgat tgaaaaatgg agatagatat gttatactgg gaacagtgaa atgcgtaaag
      481 tgcataagga ggcgatttgc tttaatttga acctttaaaa ttatatagaa aggaaagtgt
      541 cgatgaagac aagaaaacaa gagaggaaga aaaccgtatt ggtttgggta accaatcccg
      601 atgcctgcca aagtatcgtc agcgcaggaa gaaaactggc tgatgataac aaggtggaac
      661 ttgtaattgt tagcatacag ggaagcatca agggcgattg gaacaatcgc gcaagcgacc
      721 ttaagaaact aagcgaagct gcaaaaagtg cggatgccga acttacagtt gtatactcag
      781 atgacatgta tgaggcagcg cacgatacta taaaaagatt caaaccaatg attatggtga
      841 ccggtctccc gggcaatttg ggagcagggt cgtttttgga acagataatg aacatggcgc
      901 ctggtgtgcc gatatactca gtagacatga aggaaaatat gaagaggctg gatacgataa
      961 gtctcagaat atcataaata gaaataatga ggcgggctgt gcccgccata ttgtttgtca
     1021 taaatcattc gtgttttgcg taatatttag caaacaaggt tttggagctt attatgaaac
     1081 tgatatacat aaaacacggc aggcaatttt taattatttc tcttgtcctg gttattgtaa
     1141 tgatgctggc ggttgtgtcg atggtaaagg atcatttggg aagggctcag gaaacccttc
     1201 tacccatata tggtacggac gatgataaca tgagaatctc tcttacaatt aactgtgcct
     1261 ggggtgctga tgacatacct gagatacttg atattctgga cagggaacag gtaaaggcta
     1321 cgttttttgt tctaggaatc tgggcggaga agaatcctga gatgcttaaa ttgatacatg
     1381 acagaggtca tgagattgga aatcattcat attcccacaa gctgccctcc agaatatctg
     1441 tgaaacagat ggaagaggag atagacaagg caaatgatgc aatagagaag attgtaggat
     1501 tcaggccttt gttatacagg gctccttcag gagaccatac ggaggagctt ttgacgctta
     1561 cccagaggaa aggattgtat actataaaat ggactgttga cagcatagac tggaaagatt
     1621 cgatgactgc ccagaacatt atagacaggg tcacaggaag aacaaggtcc ggcagcatac
     1681 ttcttttcca taatgacaca aaacatacaa aggatgttct gccggtcatc atcaagcatt
     1741 tgaagaatga aggctatgat tttgtcaagg tttccgaact tatatacaag accgattact
     1801 acatagacca ccagggaatc cagagaggca aaagaggtta aaaagtaatt gcattaacct
     1861 ttgttattgt atataatgga agtcgccata ggaaaaaact tgttttaatt acaacagaga
     1921 agggagtttt tttgtgtcag ctgaacttgc agagaataat gttgcgtcaa tatcaggaat
     1981 aatatcctcc gaaattgaat acagtcataa gatatatgga gagtcgtttt acaattttta
     2041 tgttgtcgta atgagactga gcaaaagtta tgatacaata ccagtcacaa tttccgaaag
     2101 aattgtacct atggaaaaac ttcaaaaggg taaatatgtg gaagtgaacg gccagtttag
     2161 atcatacaac atagcggacg gggaatccag gttgatgctt accttattcg taaaggatat
     2221 caatattgct gatgcaaaac cggataagcc ggatgtgaat cagatagaat tgaatggttt
     2281 tatctgcaaa cagcctgttt acagagttac tccctttaac c
//
LOCUS       DAONVY010000385         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31143_length_2321_cov_3.483230, whole genome shotgun sequence.
ACCESSION   DAONVY010000385 DAONVY010000000
VERSION     DAONVY010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31143_length_2321_cov_3.483230"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PLI11_09255"
     CDS             complement(<1..1159)
                     /locus_tag="PLI11_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPZ53094.1"
                     /translation="MKTTDLRNVVLLGHGGVGKTTLAEAMLFNAKAIDRLGRIADGNT
                     TLDYDAEEQKRQVSINTAVAPIEWKKTKINIIDTPGYFDFVGEMYEGIFVADSAVIVV
                     AGDVAVGTEKAFDACELHNIPRVFYVNKMNEETSDFNKIYDELVNIFGNKCVAFHLPI
                     KVDRKFEGFVDVLNHKAYKFDMDGKVTETDIPSNLQAQVEELNMKIMEIVAETDDELM
                     MKFFEGESFTPEEIKKGVKEAINAKNCYPVLLGSAFENMAVGQLNDFIVDYLPNPAEI
                     CKFEAEDASGNKIEITPDSSADPVLFVFKTIADPFVGKLSLFKVCTGTVTGDTTLHNP
                     NKGTEEKISSVFTMMGKKQIPVQQLTAGDIGAVAKLSNTNTNDTLCLKSKPV"
     gene            complement(1194..1850)
                     /locus_tag="PLI11_09260"
     CDS             complement(1194..1850)
                     /locus_tag="PLI11_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPZ53095.1"
                     /translation="MDGNKKISIAVIKRLPRYYRYITELLENGITKVSSQELSRRMNL
                     TASQIRQDLNCFGGFGQQGYGYNVEVLHKEIASILGIPDPFNVIIIGAGNMGQAIAKY
                     ENFNKRGFVFTGIFDKNPNVIGQDINGVKVAHIDTLEDFVKENKTDIALLCIPKENAM
                     EIARRVAALGIHGLWNFSSTDLHNIEEEFGTVVENVHLTDNLMVLGYRISELRKKGKN
                     "
     gene            1972..>2321
                     /locus_tag="PLI11_09265"
     CDS             1972..>2321
                     /locus_tag="PLI11_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPZ53096.1"
                     /translation="MTIFSCTNINLSFGNLTIFDNFDINIYEKDKVGIVGVNGAGKTT
                     FVKLLLGMLPPDKGNVFVSSKVEIGYLEQNSGLDSNNTVMDEFLLPFSHLIELENRIK
                     SIERELQKENTDKI"
BASE COUNT          693 a          486 c          365 g          777 t
ORIGIN      
        1 ttacaggttt tgatttcaag cacaatgtat cgtttgtatt tgtgttggac agttttgcaa
       61 cagctccaat atcacctgca gtaagctgct gtactggaat ctgctttttg cccatcattg
      121 taaacacact tgatatcttt tcttcagtac ccttgttggg gttgtgcaat gtggtatcac
      181 ctgtcacagt acctgtgcaa actttaaaca acgacaattt gccaacgaac gggtctgcaa
      241 ttgtcttgaa tacaaacaat acagggtccg ctgagctgtc tggtgtaatc tcaatcttgt
      301 ttccgctggc atcttcggct tcaaatttgc atatctcagc aggatttggc aaatagtcta
      361 ctatgaagtc attaagctgg ccgactgcca tgttctcaaa agcggaaccc aaaaggacag
      421 gataacaatt cttggcgtta attgcctcct ttacgccctt cttaatctct tcaggagtga
      481 aggactcacc ttcgaagaac ttcatcataa gttcatcatc ggtctctgca acaatttcca
      541 ttatcttcat gttgagttct tcaacctggg cctggagatt tgaaggaatg tcagtctctg
      601 taacctttcc gtccatatca aatttgtatg ctttgtgatt tagtacatca acaaatcctt
      661 caaattttct atccactttt attggcaaat gaaaagcaac gcatttattt ccaaagatat
      721 taacaagctc gtcgtaaatc ttgttaaaat cagaagtttc ttcattcatt ttgtttacat
      781 aaaataccct gggtatatta tgtaactcac acgcatcaaa agctttttca gttccaacag
      841 ctacatcgcc cgcaactact atgactgcag aatcggcaac aaaaatacct tcatacattt
      901 ctcctacaaa gtcaaaataa ccgggagtat caataatatt gatttttgtt tttttccatt
      961 caatgggggc tacagcagta ttaatagata cttgcctttt ttgttcttcc gcatcgtagt
     1021 caagcgttgt attgccatct gctatacgac ccagtctgtc tatagctttg gcattgaaca
     1081 gcatagcttc agcaagagtg gttttgccga ctccaccatg tcccaataat acaacgttgc
     1141 gtaaatcagt agttttcata cacattcctc ctagaaatta aaatttattc aatttaattt
     1201 tttccttttt ttcttaattc agaaattcta taacctaaaa ccattaaatt atctgtaaga
     1261 tgaacatttt ctacgaccgt tccaaactct tcttcaatgt tgtgcaagtc ggtagatgaa
     1321 aaattccaca agccatgtat accaagagca gcgactcttc ttgctatttc cattgcattt
     1381 tccttgggaa tacacagtaa agcaatatct gttttgtttt cttttacgaa atcttcaaga
     1441 gtatcaatat gagcaacctt aaccccattt atatcttgac caataacatt gggattcttg
     1501 tcgaatatac cggtgaatac aaaacctctt ttattaaaat tctcgtactt tgcaattgct
     1561 tgtcccatgt taccagcacc gataataatt acattaaaag gatcaggaat tcctaaaatt
     1621 gaagcaattt ctttgtgaag cacttccacg ttataaccat aaccttgttg accaaaacct
     1681 ccaaaacaat tcaaatcctg tcttatttgc gaggctgtta aattcatacg tctacttaat
     1741 tcttgcgatg acaccttcgt gatcccattc tcgagcaact ctgtaatata tctgtaatac
     1801 cttggtaatc ttttaattac cgctatagag atctttttat tgccatccat ctgcatcaat
     1861 ccttttttta tgttttctca ggttaaaagc tcatgtacga tttattctat catttgttat
     1921 taacacttgt caatattgtt tttaaattgt aaaatatcac gggaggttta tatgactata
     1981 ttttcatgca caaacataaa cttgagtttt ggaaatttaa caatatttga taattttgat
     2041 ataaacatat atgaaaaaga caaagttgga atagttggtg taaatggagc tggcaaaacc
     2101 acttttgtga aactactact cggcatgctt ccgcccgaca agggaaacgt tttcgtatcc
     2161 agcaaggttg aaatcggata tcttgaacaa aactccggcc ttgactcaaa caacactgtc
     2221 atggacgagt ttcttctacc cttttcccac cttattgaac tggaaaaccg aattaaatca
     2281 attgaaaggg agctgcaaaa ggaaaatacg gacaagataa a
//
LOCUS       DAONVY010000386         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31199_length_2318_cov_3.381794, whole genome shotgun sequence.
ACCESSION   DAONVY010000386 DAONVY010000000
VERSION     DAONVY010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31199_length_2318_cov_3.381794"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLI11_09270"
     CDS             <1..440
                     /locus_tag="PLI11_09270"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPZ53097.1"
                     /translation="EYLNLPGFPAGVIMPLSGIWFYQGGINFFFAYVVSILAGLCGSW
                     VLYLIGFYGGTLVITKYTSKFPKHKERIEKVIKSIERKGYWGVFIGKLIPVARTLVSL
                     PAGALRLNFYKYTFASGVGIAIWNFVLIGAGYLFGQPVINYLS"
     gene            496..1464
                     /locus_tag="PLI11_09275"
     CDS             496..1464
                     /locus_tag="PLI11_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ53098.1"
                     /translation="MLNIALIGTWHVHFDQYAREIKENPNTKIHGIWDPDYSKAEEKA
                     KEYGTFAFDNLDDALLSSDVDGIVISTATNMHTGIIIKAAENKKHVFTEKVLCFTVEE
                     AERIMESVKRNNIKFCISYPWRTRSDFLWIKEALDNKLVGDVTYLRIRNCHDGASTGW
                     LPPHFFDLKECGGGAMMDLGAHPMYLIRWLLGRPKSITSTFTKVTGKEVEDNAVSVLE
                     YENGAIAVSETGFVSSHNPFSFEASGTKGTIYAGGFSNKCVYNIGEGWIEPKLKEPLP
                     IPTTLWVNGIIDNMDIPFDIDSAYDLTELMDYAYKSYESGTKVDLY"
     gene            1503..1904
                     /locus_tag="PLI11_09280"
     CDS             1503..1904
                     /locus_tag="PLI11_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HPZ53099.1"
                     /translation="MICNSCGTVNNEDAKFCKNCGSKLVQGEPQKPQQTQPMPNQNIQ
                     YVNPERLPKVENYLVFSILVTLFCCLPLGIAAIVKSSQVDKELAMGNYEGALAASKQV
                     KTLNLIGVIVTVVAAIIWSVVMFILPFIITI"
     gene            2028..>2318
                     /locus_tag="PLI11_09285"
     CDS             2028..>2318
                     /locus_tag="PLI11_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HPZ53100.1"
                     /translation="MVCKACGHINQDNMAKFCSACGSELQQDTQNNVYQQNVTPQPPP
                     QYVPNYLIFSILTAIFCCLPIGIAAIIKSSQVDKKIALGLYDEAITASRQAKT"
BASE COUNT          729 a          447 c          487 g          655 t
ORIGIN      
        1 tcgaatactt gaacctgcca ggttttccgg caggtgtcat tatgcctctt tcaggcattt
       61 ggttctatca aggaggcatt aactttttct ttgcatacgt agtatccata ctggcaggat
      121 tatgcggcag ctgggtacta tacctgatag gattttatgg tggtacgctt gttatcacta
      181 agtatacaag caagtttcca aaacataaag aaaggataga gaaggttata aaatccattg
      241 agagaaaagg atactgggga gttttcatag gaaagctgat tcctgttgca agaactcttg
      301 tgtcacttcc tgcaggtgcc ctaaggctta acttttacaa atacactttt gcttcagggg
      361 ttggtattgc aatttggaac tttgttctta ttggcgcagg atatttgttt ggacaacccg
      421 ttattaatta tctttcctag tgtgatataa gttccatgtg tgatataatc aagaaaacga
      481 attaggggga ttattatgct gaatatcgct cttattggca cctggcatgt tcactttgat
      541 cagtatgcaa gagaaatcaa agaaaatcca aacaccaaga ttcatggcat ctgggatccg
      601 gattacagca aggctgagga aaaagcaaaa gagtatggaa cctttgcatt tgacaatctt
      661 gacgatgctc ttctttcgtc tgatgtggac ggcattgtca tatccacagc cacaaacatg
      721 cacacaggta taattattaa ggcggcagag aacaagaagc atgtttttac tgaaaaagtc
      781 ctgtgtttta ccgttgagga agcagaaaga ataatggaaa gcgtaaaaag gaacaacatt
      841 aaattctgta tttcctatcc ttggcgcaca cgcagtgatt ttttgtggat aaaggaagct
      901 ttggataata aacttgtagg ggacgttaca tatttaagaa ttagaaactg ccacgatgga
      961 gcttcaacag gatggcttcc tccacatttc tttgacttga aagaatgcgg tggcggagca
     1021 atgatggacc tgggtgcaca tcctatgtat cttatccgct ggcttttggg caggccaaaa
     1081 tcaatcacat ccacctttac aaaagtcaca ggaaaagaag tcgaagacaa tgcagtatcc
     1141 gtacttgaat atgaaaatgg cgccatagcc gtaagcgaaa cgggatttgt ttcatcccac
     1201 aatccatttt catttgaagc cagtggtaca aagggaacga tatacgcagg cgggttttca
     1261 aacaagtgcg tatacaatat cggcgaagga tggatagaac caaaattgaa agagcctctg
     1321 cccataccta caaccctttg ggtaaatgga ataatagaca acatggatat accctttgac
     1381 attgacagtg cgtacgatct gaccgaactg atggattacg catacaagtc atatgaaagt
     1441 ggtaccaaag ttgatcttta ttgatttgaa tataacttta tatttacaag gaggaattct
     1501 atatgatatg caatagttgt ggtactgtaa acaatgagga tgcaaagttc tgcaaaaact
     1561 gtggaagcaa actcgtgcaa ggcgaacccc agaagcctca gcaaacccaa ccaatgccaa
     1621 atcaaaacat ccagtatgta aacccggaaa gactgcctaa agtggaaaac tatctggtct
     1681 tttcaattct tgttacactc ttttgctgtc tgcctcttgg cattgcagca atagtcaagt
     1741 ccagccaggt agacaaggag cttgccatgg gcaactacga aggagcattg gctgcatcca
     1801 aacaggtaaa gactctcaac ctgataggag tgattgtcac agtagttgct gcaattattt
     1861 ggtctgttgt catgttcata ttgcctttta ttataacaat ttaaccttta catttttatc
     1921 aaaactagca ggtcttacac cttctagttt ttattactga ttgacttttt ctgttaaagc
     1981 ctctaaaatc aagaatacat gtatatattt tttcaggagg acaagaaatg gtatgtaaag
     2041 cttgtggcca tatcaatcaa gacaatatgg caaaattctg ttcagcatgt ggaagcgaac
     2101 ttcaacagga cacacaaaac aacgtgtatc agcaaaatgt tactccccag ccgccaccac
     2161 aatatgtgcc aaactatttg attttttcga tccttacagc aatcttttgc tgcctgccca
     2221 ttggtattgc agcaattata aaatccagcc aggtagataa gaaaatcgct ttggggctgt
     2281 acgatgaagc aataacagca tccaggcagg caaaaacc
//
LOCUS       DAONVY010000387         2313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31295_length_2313_cov_4.817095, whole genome shotgun sequence.
ACCESSION   DAONVY010000387 DAONVY010000000
VERSION     DAONVY010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31295_length_2313_cov_4.817095"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..966
                     /locus_tag="PLI11_09290"
     CDS             175..966
                     /locus_tag="PLI11_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPZ53101.1"
                     /translation="MTKEEFLVNRKKDREFIDLLIEEEKKHFEGWDFSYLESTNRMQE
                     FPLSWNYWHEVEKRMNRCSSLLDMGTGGGEFLSSLAPLPKIVCATEGYEPNIPIARKR
                     LEPLGVKVYKVDSDENLPFDNYFFDLVINRHESYSEKEVHRVLKKGGIFVTQQVGGLN
                     DKEINELLGVPLPEYVGWNLIAAVARLKENGFNVTLEKEEFVKTRFYDVGAIVFYLKA
                     IPWQVPNFTIKRYLEKLKDIDRIIQKDGYIDFTCHRFLVVADKKQ"
     gene            1262..>2313
                     /locus_tag="PLI11_09295"
     CDS             1262..>2313
                     /locus_tag="PLI11_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPZ53102.1"
                     /translation="MSTRVFKVTDYGVVPGKEELQTEALQRVFDMCKDEGGTVVIPSG
                     KYRTGGLRMWSDTTLLLESGAYLLGSDICEDYPVFEVPEGVELRTDMELIPNYYNDKP
                     WEEYRRAIISVYGGKNISIIGEPDSVIDGDDCADPNGEEGYRGPHGIFITNVNNLLLE
                     GYTIQHFGNFAHQIDNCTNVTARGLKCYGGSDGFHLHFCRNTLIEDCIIHTGDDCIGG
                     INVEGLVVRNCNMNTSCDAFRMGGSHILVENCHIWGPGIYPHRMTVVQNRYTEAVRRK
                     DNTLPREAGRHNMVRIWLHFASEVFPSEEPYHDITFRNCIIENSDIFLTYQPNEILQR
                     GAELKEMFLDNVKIMGV"
BASE COUNT          744 a          364 c          570 g          635 t
ORIGIN      
        1 ttgctgtttg agtatctttt caattttgta gatagaattg tatgggatac attgattgaa
       61 aatacaaggg cacagcatgt ctatgagagg aaaattggag caagaagagt aggtacaagg
      121 gagaattgct ggaaagacca gaccggcaaa tatagaacgg ctgttgatta tgaaatgaca
      181 aaggaagagt ttttggttaa tagaaaaaag gacagggagt ttattgattt gttgattgaa
      241 gaagagaaaa aacactttga aggatgggat ttttcgtatc ttgaatcaac aaacagaatg
      301 caggagtttc cgctttcgtg gaactactgg catgaggtag agaaaaggat gaaccgctgt
      361 tcttcgttat tggacatggg tactggtgga ggagaatttc tttcatccct tgccccgctg
      421 cccaaaattg tctgtgctac tgaaggttat gaaccaaaca taccaatagc acgaaagagg
      481 ctcgagccct tgggcgttaa agtctataaa gtggatagtg atgagaatct tccatttgat
      541 aattattttt ttgatttggt tataaacagg catgaatcgt attctgaaaa agaggtacat
      601 agagtactta aaaaaggcgg gatatttgtt acacagcagg taggtggcct taatgacaag
      661 gaaatcaatg agctgttagg tgtgcctttg cccgagtatg tgggatggaa tttgattgct
      721 gctgttgcca gattgaagga aaatgggttt aatgtaactt tggaaaaaga ggaatttgtg
      781 aaaaccaggt tttatgatgt tggtgcaatt gtgttctatt taaaagccat accctggcaa
      841 gttccgaatt ttacaattaa aagatatctg gaaaaactta aagatattga tagaataata
      901 caaaaggatg gatatataga ttttacatgt cataggtttc tggtagtggc agataaaaag
      961 caatgagcgg gatattgaat ttgaatagat aacacgggtt gacatagaag taaatgaaaa
     1021 acaaatcaat gggcaaaaac gtaatggcgt agaccttggg cttaaggtgc cagtggtagc
     1081 aacaacaagc aatggtaaaa caaagtttat tgataagatg cgtttggtgt ttttatattc
     1141 ttattcctga accatctcct cttcctgaaa gattggcaag aaatcttctt caatagacag
     1201 aatgcatatc atttggtaat attatgcgca aaagcaaatg tattaacatg ggggtatttg
     1261 aatgagcaca cgagttttca aagttaccga ttatggagtg gttccaggca aggaagaatt
     1321 gcaaaccgaa gcattgcaaa gagtttttga catgtgtaag gatgaggggg gcactgtagt
     1381 tataccatca ggcaaatatc gtactggagg tcttcgtatg tggtcggata cgacactcct
     1441 tcttgaatcc ggggcatatc tcctgggctc ggacatatgc gaggattatc cagtgtttga
     1501 agtgccggaa ggtgtggaac tgcgtacgga catggaactt atcccaaact attataacga
     1561 caagccttgg gaagagtaca gaagggcaat catatccgtt tatggaggaa aaaacatatc
     1621 aataattgga gaacctgatt ctgtgataga tggtgatgac tgtgcggatc ctaatgggga
     1681 agaaggctac aggggacctc acggcatatt tattaccaac gtgaacaatt tgcttctgga
     1741 aggttatacc atacagcact ttggcaattt cgcacaccag atagacaact gtacaaatgt
     1801 aactgcaaga ggtcttaaat gctatggagg aagcgacgga ttccatctcc atttctgcag
     1861 aaatacattg attgaagact gcatcataca tacgggtgac gactgtattg gtggaattaa
     1921 tgtggaagga ctggttgtaa ggaactgtaa tatgaacacc tcctgtgatg ctttcagaat
     1981 gggtggaagt catatccttg tagagaactg ccatatctgg ggacctggca tttatcctca
     2041 tagaatgact gttgtccaga accgttacac agaggcggtc agaaggaagg acaacaccct
     2101 tccaagagaa gcgggaagac acaacatggt aagaatatgg ctgcactttg ccagtgaggt
     2161 ttttccctct gaagagccat atcatgatat aactttcagg aactgcatta ttgagaactc
     2221 agacattttc cttacctatc agccgaatga aatccttcaa agaggagcag agctcaagga
     2281 aatgtttctc gacaatgtca agatcatggg tgt
//
LOCUS       DAONVY010000388         2308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31380_length_2308_cov_3.553040, whole genome shotgun sequence.
ACCESSION   DAONVY010000388 DAONVY010000000
VERSION     DAONVY010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31380_length_2308_cov_3.553040"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLI11_09300"
     CDS             complement(<1..485)
                     /locus_tag="PLI11_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPZ53103.1"
                     /translation="MIGVSQKAIARIRRELFKSIQRLPVKYQDTHTHGDLMSRFTNDL
                     DSVGVMLNSTMSQIFSGTITLIGTIVLMFVTNWILAIVTIVTVPLLIYAANTLGKMSR
                     RYYSEKVFCQEETAKQEFEILSDDLREKQIKAQFFGGIMGPVMGNLSQISYALSATSA
                     L"
     gene            complement(448..921)
                     /locus_tag="PLI11_09305"
     CDS             complement(448..921)
                     /locus_tag="PLI11_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPZ53104.1"
                     /translation="MTLDKHIIHQLITFAIKHRKIMQNYLDATGVYHAQHRLLMEISR
                     NPNAPQIELERQMDVSSATIAVSLKKLEKGGYIIKKTDDVDNRLNQIVITEKGYCVVE
                     KSKKIFKMADEKCFKGFSEEEKKNFLQLLEKLNSNIFVPAAENNDWRVSESHCPY"
     gene            1078..1767
                     /locus_tag="PLI11_09310"
     CDS             1078..1767
                     /locus_tag="PLI11_09310"
                     /inference="COORDINATES: protein motif:HMM:NF020374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1796 family putative cysteine peptidase"
                     /protein_id="HPZ53105.1"
                     /translation="MENYQHVISLGHFCAVASELERLGYRDASLPFDWLITPFESVMH
                     MIETNFNDFLVLENLKRDEKRKYIIKDTKYNIAFYHDYKPNQSIETQHEIVKAKYDRR
                     IKRFYDYITEKTLFIRYIESKKEYEFICENYDHIMSVLRKYNKDNDLMLVSKDIKAVE
                     LPMHTIYLDNVPYKTPQKFAKTNKELYKVLTSIPYPDEKRMLNINRYRKSFPKRFFNS
                     LRKLYIKLIYS"
     gene            complement(1754..>2308)
                     /locus_tag="PLI11_09315"
     CDS             complement(1754..>2308)
                     /locus_tag="PLI11_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPZ53106.1"
                     /translation="EYYSHLLAESIYLQRLVNELLDLSRLQNADFSLNKEEVNLLSIV
                     HESVRSARRLCTEKDISINFESDMDVEVINGDFDRLRQMILIILDNAIKFSSTGSTID
                     ITYRNFILSITDHGEGIREEDLPYIFDRFYKMRDEKNKEGTGLGLAIAKQIALRHDIA
                     VTVDSKENEYTTFSFDFSNIVSYK"
BASE COUNT          723 a          465 c          390 g          730 t
ORIGIN      
        1 tgcagtgcgg aagtagcaga caaggcgtag ctaatctgac tcagattgcc catgacaggc
       61 cccatgatac caccaaagaa ttgtgcttta atctgcttct ccctcaagtc atcgctgaga
      121 atttcaaact cctgctttgc tgtttcttcc tggcaaaata ctttctctga gtagtacctt
      181 ctactcatct ttcctagcgt gttggctgca tagatcaata aaggcaccgt aactatcgta
      241 actattgcga gtatccagtt ggtaacgaac ataagtacaa tcgtaccaat gagagttatg
      301 gttcccgaga aaatctgtga catggtactg ttgagcatca caccgaccga gtccaaatca
      361 tttgtaaaac ggctcataag gtcgccatga gtgtgagtgt cctgatattt cacaggcagt
      421 ctctgaatgc ttttgaacag ttctcttcta atacgggcaa tggctttctg agacacgcca
      481 atcattattc tctgctgcag gtacgaatat atttgaattc agtttttcaa gcagttgtaa
      541 aaagtttttc ttctcttcct cagaaaaccc tttaaaacac ttttcatccg ccattttgaa
      601 tatcttcttg ctcttttcaa ccacgcaata tcctttttca gtaatgacaa tctgattaag
      661 ccgattgtcc acatcatcag tctttttaat aatatatcca cccttctcca gcttctttaa
      721 ggataccgca atggttgcgg aagatacatc catctgtctt tccagctcaa tctgaggcgc
      781 attggggttt cgtgatattt ccataagcag cctgtgctgc gcatggtaca cacctgtggc
      841 atccaaatag ttttgcatta tttttctgtg cttaatcgca aaagttataa gctgatgaat
      901 aatatgctta tccaaagtca ttttatcacc tcatgaaaat aaatgttagc aagctaataa
      961 ttggctagct aattatttag tctatcacta aaacatttct ttgtatcaat cagtgatatt
     1021 tgaccagaat aaatcaatta tgataaactt gtacaataac aaattgcagg tgaggttatg
     1081 gaaaattatc agcatgtaat atcattgggt catttctgtg ctgttgcttc agagcttgaa
     1141 agactgggat atagggacgc aagccttcct tttgactggc ttataactcc ctttgaaagc
     1201 gttatgcaca tgatagaaac aaactttaat gattttcttg tccttgaaaa tcttaaaaga
     1261 gatgaaaaac gtaaatatat aatcaaggac acaaaataca atatagcctt ttaccatgac
     1321 tataaaccga atcaatccat tgaaacacag catgaaattg taaaggcaaa atacgataga
     1381 agaataaaga ggttttatga ttacataacc gaaaaaaccc tgtttatccg ctacattgaa
     1441 tcaaaaaagg aatatgaatt catttgtgaa aactacgacc acataatgag cgtgttgcgg
     1501 aaatacaata aagacaatga tcttatgctc gtgtcaaagg atattaaggc tgtagaactg
     1561 cccatgcata caatctatct tgacaatgta ccgtataaaa ctcctcagaa gtttgccaag
     1621 accaataagg agttgtataa ggtactaact tctattccct atccggatga gaaaaggatg
     1681 ttgaatataa atagatacag aaaaagcttt ccaaagcgct ttttcaacag cttaagaaag
     1741 ctttatatca aacttattta tagctaacaa tatttgagaa atcaaaactg aaggttgtat
     1801 actcattttc tttgctgtcc acagttacgg caatatcatg tcttaatgct atctgctttg
     1861 caatagctag accaaggccg gttccttcct tgtttttctc atcacgcatt ttgtagaatc
     1921 tgtcgaagat atacgggagg tcttcttccc ttataccttc tccatggtct gttattgaca
     1981 agataaaatt tctgtacgta atgtcaatag tggatcctgt acttgaaaac ttaatcgcat
     2041 tgtcaaggat aatcagtatc atctgtctta acctgtcaaa atcaccattt atcacttcca
     2101 catccatatc gctttcaaag ttaattgata tatccttttc ggtacaaagc cttcttgcac
     2161 tcctgacaga ctcatgtaca atactcaaga gattaacttc ttccttgttt agtgaaaaat
     2221 cagcattctg aagtcttgaa aggtccaaca attcattaac aagtctttgc agatatatgc
     2281 tttcagcaag aagatgagag tagtattc
//
LOCUS       DAONVY010000389         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31497_length_2301_cov_4.175868, whole genome shotgun sequence.
ACCESSION   DAONVY010000389 DAONVY010000000
VERSION     DAONVY010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31497_length_2301_cov_4.175868"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1357
                     /locus_tag="PLI11_09320"
     CDS             191..1357
                     /locus_tag="PLI11_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPZ53107.1"
                     /translation="MIKLLIKKLIPDYQKTHLPDVRKKYGFLAGVLGIAINLILFSAK
                     LFIGLAMHSIAIISDAFNNLTDTSSSLFAIIGAKLATKKPDKEHPFGHGRIEYIVSLI
                     ISALIFAVGFELARTSLAKIINPEKIVFNPALVVFLSFSILLKIWMFSYNRYMGKLIN
                     SKLLKTAATDSLNDVFATSAVIVSTLISYYLNINADGYIGMVVSLIIIKSGFDTAKEV
                     IDILIGSPPSPELVTMIGDILSNVDGIYGFHDLVVHEYGPGNFIASVHVEVPDHLSVV
                     KIHETIDLTEMKIHEDLGVDIVIHMDPISLNDEKTNKYKNIVNELVKNINPEFSIHDF
                     RITDGENRVNLLFDLVVPFNMSSKEKTHVAEKINKEVKELDGRIRCFIRFDNPS"
     gene            complement(1354..2124)
                     /locus_tag="PLI11_09325"
     CDS             complement(1354..2124)
                     /locus_tag="PLI11_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53108.1"
                     /translation="MRKILCFVIIATVLCLGAAHAEEDYKIYYFTDASIADVINNSEL
                     TKNATAQVSDEILKISLTKGAQDPHFMLPVEGINAVGGEDNILAICVKTTFRTFEYDY
                     AYATFVTDEGEGVVTANSYAVTERWQVIFFRLGSNENYKGNLKSIRIDPFENAPDKDE
                     DLLYQIKWFGFFKDINSANEFANDYLNEINPTMAPGETEVPHKTSPPPEPYGEDNSIW
                     GFVWTIVAMLAATVVITYFLEKMRKQETEKSKTKKKKR"
BASE COUNT          677 a          448 c          460 g          716 t
ORIGIN      
        1 ctttacgaag agttgaagga taaagtggaa gttcacgtaa ttggagatgc aaaacaggtt
       61 cgcaaagcag ttcaggcaat agaggaaggc gcaagactgg cgcttgaaat ctagttaagt
      121 attggatttg acaaacccag gtatcggctt ccgatacttg ggttaatttt atatcttgga
      181 aaggcgaaag atgataaagc tactgattaa aaagttaatt cccgattacc aaaaaaccca
      241 tcttcctgac gtaaggaaaa aatatggttt tcttgcagga gtgctgggta ttgccataaa
      301 cctaatattg ttttctgcaa agctttttat tggccttgca atgcacagta ttgccataat
      361 ttcggatgcc ttcaacaatc ttaccgacac cagctcgtca ctttttgcaa taattggagc
      421 aaaacttgca accaagaaac cagataaaga gcatcctttc ggacatggaa gaatagagta
      481 tattgtatca ctcattattt ctgcattaat ttttgctgtt ggatttgaac ttgccaggac
      541 atcccttgca aagataataa accctgaaaa aatagttttt aatccggctt tggttgtttt
      601 tctatccttc tctattctgc tgaaaatttg gatgttttcc tacaacagat acatgggaaa
      661 acttattaat tcaaaattgt tgaaaacagc agcaacagac agtttgaacg atgtctttgc
      721 aacctccgct gtcatagtgt caactctaat ttcgtattac ctgaacatta atgcagacgg
      781 ttacatcgga atggttgttt cgttgatcat aataaagtca gggtttgata ctgccaagga
      841 agtaattgat atactgattg gttcgcctcc aagccccgag cttgtaacaa tgattggtga
      901 tattcttagc aatgtggatg gaatctacgg ttttcatgat ttggtggtgc atgaatatgg
      961 acctggcaac tttattgcat cggttcatgt ggaagtacca gatcacctca gcgtggtcaa
     1021 aattcacgag accattgacc ttactgaaat gaaaatacac gaggatctgg gtgtggatat
     1081 tgtaatacac atggacccca tatcactgaa tgatgaaaaa acaaacaagt acaagaatat
     1141 agttaatgaa cttgtgaaaa acataaaccc tgaattcagc atccatgatt tcagaataac
     1201 ggatggtgaa aacagggtca atctgctttt tgatttggtg gttcctttca acatgtcaag
     1261 caaggaaaag actcatgtag ctgaaaagat aaacaaagaa gtcaaagaac tcgatggaag
     1321 aatccgctgc tttattcgat ttgacaaccc aagctagcgc ttcttctttt ttgtcttgct
     1381 tttctccgtt tcctgctttc tcattttttc caaaaagtaa gtaattacga cagtagctgc
     1441 aagcatggca actatcgtcc atacaaatcc ccatatggaa ttatcttctc cgtagggttc
     1501 cggtggtgga gaagttttgt ggggaacttc agtctcaccc ggtgccatcg ttgggtttat
     1561 ttcattaagg taatcgttcg caaactcatt tgcactgtta atatccttga aaaacccgaa
     1621 ccatttaatc tgatacaata aatcttcatc cttgtcggga gcattctcaa agggatcaat
     1681 tctaatggat ttaaggttgc ctttgtaatt ctcgttggag ccgagcctga aaaagattac
     1741 ctgccagcgt tcggtaaccg cataggagtt tgcggtgact actccctctc cctcatccgt
     1801 aacaaaagtg gcgtacgcat aatcgtactc aaaagttctg aatgtggtct tgacacatat
     1861 ggcaagtatg ttgtcctcgc cacctacggc attaatgcct tcaaccggaa gcatgaagtg
     1921 ggggtcctgt gctcccttgg tcaaggatat tttcaatatt tcatccgata cttgcgcagt
     1981 tgcgtttttt gtaagctctg aattgtttat gacatctgca atggatgcat cggtaaaata
     2041 atatattttg tagtcttctt ctgcgtgtgc ggcaccaaga cacagtactg ttgctatgat
     2101 aacaaaacaa agtatttttc tcatcttatt ctcccatgaa tattttttca ctcctgaaaa
     2161 ttatatagcc aactggacaa gatgtcaatt tgtgctcgga ccagtttttt tgttacggtc
     2221 atttgaattt ttaaatgcaa agatacagtt gcatttactt tttcctaata ccaaatgcct
     2281 ttatgggtac ccgagggtac c
//
LOCUS       DAONVY010000390         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31533_length_2299_cov_3.694742, whole genome shotgun sequence.
ACCESSION   DAONVY010000390 DAONVY010000000
VERSION     DAONVY010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31533_length_2299_cov_3.694742"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLI11_09330"
     CDS             <1..342
                     /locus_tag="PLI11_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPZ53109.1"
                     /translation="QSIPVDGIVIVTSPQELVSMIVAKAAKMANLMNIPILGIVENMS
                     YFECPDCGGRHSIFGDSHIEDIAKEYNIDTIDKLGINPKIASAVDKGMIELFDGNGLE
                     NVQTKIEGLIR"
     gene            complement(331..1059)
                     /locus_tag="PLI11_09335"
     CDS             complement(331..1059)
                     /locus_tag="PLI11_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HPZ53110.1"
                     /translation="MTKKAHITLASHIADRIGLKGNKYRKFIFYSGNIWPDVSIMFLI
                     KRHYITNTFRHLERLINWLNNRKEMLSKEELLKGRYLLHLGKVNHFIADYFTLPHNIT
                     FEGVKAHLIYERLLLQSLSEITENGKVEEVGSIEAKNCETIDTYGAKDYYSLPDEAAA
                     TYYSRDDYHRIGELIRKVKKDNNTLSILKFIIASHYEYLMKVKEAILLDCPSNKKAKT
                     LVDLDSRYIVSVCTEAIQGIVSTN"
     gene            1255..1719
                     /locus_tag="PLI11_09340"
     CDS             1255..1719
                     /locus_tag="PLI11_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPZ53111.1"
                     /translation="MNKKAFRITTLSLSILSILFIFSNSLMNANTSSSKSKGVRELVN
                     SIINNLGLTFEITEHFVRKAAHFIEFMLLGIFLTLLLVAFDLVVHKNITIPLFIGLLV
                     AVFDEYIQLHVDGRAGMVSDVVLDFLGLVTGVFFVLAIIIIKKKASQRKPSH"
     gene            complement(1716..>2299)
                     /locus_tag="PLI11_09345"
     CDS             complement(1716..>2299)
                     /locus_tag="PLI11_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53112.1"
                     /translation="EKGWSVAEKTYEEFYADGKFKDCFVYWEGKPFMIVTSMIDNPPE
                     ELTIRRMWGLRQSLEVSEWSFLNIENIPSYDEDGFVEQIAVCAAAQETYMTEPTAHGR
                     NHGIFFYEHWKRVFEYRPKVITLTWWNEWTAQRFEDEHGNPRFVDNYNQEFSRDIEPM
                     EGGHGDQYYQWMKQYIKAYRDLEECPRLVEEGY"
BASE COUNT          648 a          465 c          429 g          757 t
ORIGIN      
        1 cagtcaatac ctgtagatgg tattgtaatt gtgacctctc cacaggaact ggtatcaatg
       61 attgtggcaa aagccgcaaa aatggcaaac cttatgaaca tacccattct gggaattgtg
      121 gaaaacatgt cctacttcga atgtcctgac tgcggtggca gacacagtat ttttggcgat
      181 agtcatatag aagacatagc aaaggaatac aacatagata ccattgacaa actgggtatc
      241 aatccaaaga ttgcatctgc tgttgacaaa ggcatgatag aactgtttga cggtaatgga
      301 cttgagaatg ttcagacgaa aatagagggg ttaattcgtt gatacaattc cctgtattgc
      361 ttcagtacat acactgacaa tatacctgga atcgagatca accaatgttt tagctttctt
      421 attactgggg caatccaaca ggattgcctc ttttactttc ataaggtatt catagtgaga
      481 cgcaataatg aacttaagaa tggacaaggt gttgttgtcc ttctttactt tgcgtataag
      541 ttcacctatc ctatggtagt catctcttga atagtatgtg gctgcagctt cgtcaggcag
      601 cgaataataa tcctttgctc cgtatgtgtc aattgtttca cagttttttg cctcaatgga
      661 gccaacctct tcaacctttc cgttctcggt tatctctgaa agggactgaa gaagcagtct
      721 ttcataaata aggtgagcct taaccccttc aaaggttatg ttgtggggca gggtaaaata
      781 gtctgctatg aagtggttta ccttgcctag atgcaaaagg tacctgccct tcaaaagttc
      841 ttcttttgat aacatttcct ttctattgtt aagccagttt ataagtctct ccaaatgtct
      901 gaaagtgttg gtaatataat gcctttttat gagaaacata attgatacgt caggccatat
      961 atttccactg tagaatatga attttctata cttgtttcct ttcagtccaa ttctgtccgc
     1021 tatatgtgaa gccaatgtaa tgtgtgcttt ttttgtcatt ccctactcct gttatataaa
     1081 aatctcaagc atattttgca catgtttgta caattaattc atggtgatta ttcacatgcc
     1141 gcaaatagta ccaaagcgtt ccaatcaagt caaaggccat attgtaatat ttttcttaac
     1201 taatcttgta gaagctgaat aattaatgta aaatatgata gaaggagtga gagtttgaat
     1261 aaaaaagcat ttcgcataac cactttatca cttagcattt taagcattct gtttatattc
     1321 tccaattccc ttatgaatgc aaatacatcc tcgtcaaaaa gcaaaggtgt aagggaactc
     1381 gtaaattcaa ttataaataa tttgggactt acttttgaga ttacggagca ttttgtaaga
     1441 aaggctgccc actttattga gtttatgctt ctggggatct ttttgactct attactggtt
     1501 gcttttgacc ttgttgtaca caaaaacatt accattcctc tttttatcgg tcttttggtt
     1561 gcagtttttg atgagtatat tcagctgcat gtagatggca gggccggaat ggtttccgat
     1621 gtggtgcttg attttttagg gcttgttaca ggagtatttt ttgttttagc aataataatc
     1681 ataaagaaaa aggcctccca aaggaagcct tcacattagt aaccttcttc aaccagcctt
     1741 ggacattctt ccaaatctct ataggccttt atgtactgtt tcatccattg ataatactga
     1801 tcaccgtgtc cgccttccat aggctctatg tcacgactga actcctggtt gtagttgtct
     1861 acaaatcttg gattgccatg ctcatcctca aatctttggg ctgtccattc attccaccaa
     1921 gtaagggtga taacttttgg cctatattca aataccctct tccagtgctc gtaaaagaat
     1981 attccgtgat tcctcccatg agcggtaggc tccgtcatgt aggtttcctg ggcggcggca
     2041 caaacagcta tctgctctac aaaaccgtcc tcatcgtatg aaggaatgtt ttcaatattc
     2101 agaaaactcc attcagatac ctcaaggctc tgacgaagac cccacatgcg tctgatagtt
     2161 aattcttcag gcggattgtc tatcatgctg gttacaatca taaaaggttt gccttcccag
     2221 tatacgaagc agtccttgaa ctttccatca gcataaaact cctcataagt cttctcagca
     2281 acagaccagc ctttttcct
//
LOCUS       DAONVY010000391         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31564_length_2297_cov_3.724353, whole genome shotgun sequence.
ACCESSION   DAONVY010000391 DAONVY010000000
VERSION     DAONVY010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31564_length_2297_cov_3.724353"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PLI11_09350"
     CDS             <1..1240
                     /locus_tag="PLI11_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53113.1"
                     /translation="LVLKASEEAEFTLLHLYQNWGSYPLKQLSWIQFHVSYYHLSTGV
                     TESNCIAPYYVYGKDGWTLPDFRGPSGIMWTSQPQFNSVGRLYFVSYMKGVKKIGSEY
                     TGTYIRSSGQTYADLDYSYISDCGSYKYTLRHLEFPQTDENRTYYSLRLEFLKDLTID
                     NVRENFSLFTFDSRGNKFYQAGYLDENNEPKTVMIDTATENTQIYKLGNQNPYFSYFE
                     LDKNEDMNFGFILKNSNIKIGKKKYSGGFVFRNTFSGTINIGSLSLDLGKTKFKKGDY
                     IELDFILLPYGSYNDLHDDNVRYVREDSALKPLKIEAIKGEVVKDAYLPRIKCVDNQA
                     EFTISGGRNNNAVRIDGFTNIKKPRIYELVDGEYVPIEFFVHEYDGYSVHFNMDGTYS
                     FSFVFEMESPDAKRTFKIEN"
     gene            1307..1837
                     /locus_tag="PLI11_09355"
     CDS             1307..1837
                     /locus_tag="PLI11_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HPZ53114.1"
                     /translation="MEKLLVVVDMQNDFIDGALGSEEAKRIVPAVVDKIKKHEGEIVF
                     TLDTHKEDYLDTQEGRNLPVVHCKEGTHGHKVHKDLLDAAKDKKYTEIVKYTFGSTAL
                     GEKVARMFEEGLREVEIVGLVTDICVLSNTLLIKAFCPEVLVTVDASCCAGSNREVHE
                     NALKAMKQCQINVINE"
     gene            1971..>2297
                     /locus_tag="PLI11_09360"
     CDS             1971..>2297
                     /locus_tag="PLI11_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPZ53115.1"
                     /translation="MKNKYTVEGMTCSACSATVHRTVQKIQGVKEVNVNLLTKSMTVE
                     LDENVDVDNIDDKIIKAVNSAGYKASVFTEGKSSRKEETGANLVKEELKEMKTRVIWS
                     FVFLIPL"
BASE COUNT          739 a          388 c          557 g          613 t
ORIGIN      
        1 gcttgtactg aaagcaagcg aggaggctga atttactctt ctccaccttt atcagaactg
       61 gggcagctat cctctcaagc agctgtcctg gatacagttc catgtttcgt actaccatct
      121 ttccacaggt gtaaccgaat caaactgcat tgctccatat tatgtctatg gcaaggatgg
      181 ttggacactg cctgattttc gcggtccaag cggcatcatg tggacatcgc aaccccagtt
      241 caactctgtt ggaagactgt attttgtctc atacatgaaa ggggtaaaga agataggcag
      301 tgagtataca ggaacgtata ttaggagttc gggtcagacg tatgcagact tggactacag
      361 ctacatatct gactgcggat cctacaaata tacattgaga catctggagt ttccgcaaac
      421 ggatgaaaac aggacgtatt actctttacg tcttgagttt ttgaaagacc tcacaataga
      481 caatgtcaga gaaaactttt cactgttcac ttttgactca agaggcaaca agttctatca
      541 ggcagggtat ctggatgaaa acaatgagcc aaagacagtg atgattgata ctgccactga
      601 gaatacacag atctacaaac tgggcaatca gaatccgtac ttctcatatt ttgaacttga
      661 taaaaacgag gatatgaact ttggtttcat attgaaaaac agcaatataa agattgggaa
      721 gaagaagtac agtggcggct ttgttttcag gaataccttc agtggaacca tcaacatagg
      781 aagcctcagt cttgacttgg gcaaaaccaa attcaagaag ggcgactaca ttgaactgga
      841 ttttattctt ctcccatatg gatcatacaa cgatttgcat gatgacaatg tcagatacgt
      901 aagagaggat tcggcgctga agcctttaaa aattgaggca ataaagggag aggttgtaaa
      961 ggatgcttat ctgccaagga tcaagtgtgt ggacaatcaa gccgagttta ccatatcagg
     1021 cggaaggaac aacaatgcag ttcgtataga tggctttacg aatatcaaga agccaaggat
     1081 ttatgagctt gtggatggtg aatacgtgcc cattgagttt tttgttcatg agtatgatgg
     1141 ttattcagta cacttcaaca tggatggaac gtatagtttt tcctttgtgt ttgaaatgga
     1201 gagtcctgat gccaaaagaa cgtttaagat agaaaactag catttcgtcc tttctccagt
     1261 ttttgtggta taatgaatta tactacttgg agaaaggaga attttaatgg aaaaattgct
     1321 ggtagtagtt gacatgcaga atgattttat agatggcgct ttaggaagcg aggaagctaa
     1381 aaggattgtt ccagcagtgg ttgataagat aaagaaacat gaaggagaaa ttgtatttac
     1441 tcttgataca cataaagaag attatttgga tacgcaggag ggaagaaatt tgcctgtcgt
     1501 acactgcaag gaaggcacgc atggccacaa ggtgcataaa gaccttctgg atgctgcaaa
     1561 ggacaagaaa tacactgaga ttgtaaagta cacctttgga agcaccgcct tgggtgagaa
     1621 agtggcaaga atgtttgaag aaggtcttag agaagtggaa attgtgggat tggttactga
     1681 tatttgtgtt ctctccaata cacttttaat taaagccttt tgccctgaag tgctggtaac
     1741 cgtggatgct tcctgttgtg cgggatcaaa ccgggaggtt catgaaaacg cgttgaaagc
     1801 catgaagcag tgccagataa atgtaatcaa tgaatagaaa aagctccgct gaaaacaggc
     1861 ggggctttta ttttttgtga atactgcctc atgtattgac aaacaataac aacaggagta
     1921 aaatagatat acccccaggg ggtgtaggta tgtaatgaga ggtgaagcga gtgaagaaca
     1981 aatatacggt tgaaggcatg acttgctcgg catgttctgc cacagtccac agaacagttc
     2041 agaaaataca gggagtaaaa gaggttaacg taaacctgtt gacaaaaagc atgactgtgg
     2101 aattagatga gaatgtggat gttgataata tagatgataa gataataaag gctgtgaaca
     2161 gtgcaggtta caaggcatcg gttttcacag aggggaaatc ctccaggaaa gaggaaacag
     2221 gtgcaaatct tgtaaaggaa gaactaaagg aaatgaagac cagggtgatt tggtcctttg
     2281 tcttcctgat tcctctt
//
LOCUS       DAONVY010000392         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_31885_length_2279_cov_3.378147, whole genome shotgun sequence.
ACCESSION   DAONVY010000392 DAONVY010000000
VERSION     DAONVY010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31885_length_2279_cov_3.378147"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLI11_09365"
     CDS             <1..785
                     /locus_tag="PLI11_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ53116.1"
                     /translation="HCQGAYAHDLRYEILTGKENRHYRLRNYIHRNCDNYPTHDLGPI
                     AKLLNINRGNKMVSLVTVASKAAGLRDYVRNNIGKLDKSLLEVDFRQGDIVHTLITCN
                     NGETILLKLDTTLPRFYARDFTVRGTKGFYEQNTNTVFIEGIHKEEFWHTPDSYRCLL
                     NNAVEFEDKYLPAIWKEATAEDLSSGHGGMDAFVFKAFVKSALNNEEMPIDVYDAASW
                     MSISCLSEESIRHMGRPMSIPDFTNGKWITRPPKDVVEININ"
     gene            820..2079
                     /locus_tag="PLI11_09370"
     CDS             820..2079
                     /locus_tag="PLI11_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPZ53117.1"
                     /translation="MQKDSIKVIVLGCGNRATAYCKAAEELGDAYEIVAAVDFKDEAL
                     EFMQKRFNVPKDMCFKDYKEVLKLGKIADCVINTTMDQYHVETAIAFLGLGYDMLLEK
                     PVANNKRDLLKVAEKARENGCKLVVCHVLRYTPFYKKAKELIEEGAVGEVVHIDACEY
                     PGVLLTDFSFIRGQWRNKDECGSTFLLAKSCHDIDLICWLNNTAKPLYVSSFGGRRFI
                     VPEKAPEGAADNCFECKHSETCIYSVGYNSVNIKRIWDILPEGGVEEELRNLRNDLKR
                     SKCAYKTDANIVDNQAVMIEFENGSMATFKLLSSGMPEVRNLFVKGTQGEIEGCLQHS
                     KIVLRKYIVKTGEHEVTEYDLTDANVFKAHHGGDEGLIADFNAYIRNEKTSSSAPVID
                     DTIAGYLCVYAADKAMEDREIVKINMA"
     gene            2103..>2279
                     /locus_tag="PLI11_09375"
     CDS             2103..>2279
                     /locus_tag="PLI11_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HPZ53118.1"
                     /translation="MGLKKIVLITIGCIFVGLGGLGLVLPVLPTTPFVLVASACFAAS
                     SPTLYNKLINSPYFG"
BASE COUNT          749 a          405 c          546 g          579 t
ORIGIN      
        1 tacattgcca gggagcctac gctcatgact tgaggtatga aatccttaca ggaaaagaga
       61 acaggcacta ccgtcttaga aattacatac atagaaactg tgacaactat ccaactcatg
      121 accttggtcc gattgcaaaa ctgcttaata taaacagagg aaacaagatg gtttcccttg
      181 tcacggtagc ctcgaaagct gcaggactta gggattatgt gagaaacaac ataggaaaac
      241 tcgacaagtc tcttcttgaa gttgatttca ggcagggtga tattgtacac acattaataa
      301 cctgcaacaa cggagagacc atcctcttga agcttgacac tacactgcca aggttctatg
      361 caagagactt tactgtaaga ggtaccaaag gtttttacga acaaaataca aatacagtat
      421 ttatagaagg aatacacaaa gaagagttct ggcacactcc tgactcttac agatgtcttc
      481 tgaataatgc agtggagttt gaagacaaat atctcccagc catctggaag gaagcaaccg
      541 ctgaagattt gagttccggg catggaggca tggatgcatt tgtattcaag gcatttgtaa
      601 agtctgcgct taacaacgag gaaatgccta ttgatgtata cgacgcagct tcatggatga
      661 gtataagctg cttgtcggaa gagtcaatca ggcatatggg aagacccatg agcatacctg
      721 actttacaaa tggaaaatgg ataactagac caccgaaaga tgttgtggaa ataaacataa
      781 attaagcaga taaagtgaag aaaaaatgga gggatataat tgcagaaaga tagcataaag
      841 gttatcgttt taggatgtgg caacagggca accgcatatt gcaaagctgc tgaggaactg
      901 ggagatgcgt atgaaatagt ggcagctgtg gatttcaagg atgaagcgtt ggagttcatg
      961 caaaaaagat tcaatgttcc aaaagacatg tgttttaaag attataaaga ggttctgaaa
     1021 cttggtaaaa ttgcggactg tgtgataaat acaacgatgg accagtacca tgtggagaca
     1081 gcaatcgcat ttctgggact gggatacgac atgcttcttg aaaagcctgt tgcaaacaac
     1141 aagcgggacc ttttgaaggt ggcggagaag gcaagagaaa atggatgcaa gcttgttgtg
     1201 tgtcatgtac ttagatacac acccttttac aaaaaggcta aagagttgat agaagaagga
     1261 gctgttggag aggttgttca cattgatgct tgtgaatatc ctggtgtctt gctcacagat
     1321 ttctcattca ttagagggca atggaggaac aaggacgaat gcggctcgac tttcctgttg
     1381 gcaaaatcct gccatgacat agaccttatt tgctggctga acaatacagc aaagcccctt
     1441 tatgtatcaa gttttggggg aagacgattt atagttcctg aaaaagcacc cgagggagcg
     1501 gcggacaatt gttttgagtg caaacattcc gagacctgca tatattctgt aggatacaac
     1561 tcggttaaca taaaaaggat ttgggacata ctccctgaag gaggggttga agaagaatta
     1621 aggaatctca ggaatgactt gaaaagaagc aagtgtgcct ataaaacaga tgcaaatatt
     1681 gtagacaatc aggcggtgat gattgagttt gaaaatggaa gcatggccac cttcaaactc
     1741 ctttccagcg gaatgcctga ggtacgaaat ctttttgtaa aaggaaccca gggagagatt
     1801 gaaggatgtc ttcagcacag caagatagtc ctgagaaaat acatcgtcaa aacaggggag
     1861 catgaagtga ctgaatatga tttaaccgat gcgaatgtat ttaaagccca tcacggcgga
     1921 gatgaaggtc tcatagccga ctttaatgca tatataagga atgaaaaaac atcatcctcc
     1981 gcaccggtta ttgatgatac catagctggt tacctctgtg tttatgctgc tgataaggcc
     2041 atggaagaca gagaaatcgt gaaaatcaac atggcttgat atgcagcagg aatgatattt
     2101 aaatgggctt gaaaaaaatt gtattgatta caataggttg tatattcgta ggacttggag
     2161 gacttgggct tgttttgcca gtccttccaa ccactccctt tgtgttggtg gcatcagcat
     2221 gctttgctgc atcaagtccc acactataca acaaacttat taacagtccg tattttggt
//
LOCUS       DAONVY010000393         2268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32093_length_2268_cov_3.890194, whole genome shotgun sequence.
ACCESSION   DAONVY010000393 DAONVY010000000
VERSION     DAONVY010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32093_length_2268_cov_3.890194"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLI11_09380"
     CDS             complement(<1..1184)
                     /locus_tag="PLI11_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPZ53119.1"
                     /translation="MKRIELLSPAGNFESLKFAVQNGADAVYLGLNKFSARASSDNFD
                     IEKLKQAISYAHPRGVLVYVAINTLLDDNDLENAIELAKMADMLHVDAFIVQDLGLAR
                     MLKKHVKAPLHASTQMTVYNEEGARLLKDLGFSRCILSRELSLDEIVTICSKNIMEME
                     VFCHGAICISYSGQCLLSSAIGGRSGNKGQCAQPCRLMYSVLRNGKEVSKRAYRISPG
                     DLMTLPYLAQLVNTGITSLKIEGRLKSPQYTGIVTAKYRKALDIIQNREIEEYPTQKD
                     MEDLSVIFSRGRFTSYHHFEKMPFVDITFSSSGHIGLECGEILKVKIFKTKGKRNSPD
                     IFELKVKLSSPVSKGDGIAIENTEDGGKVNSIKTESHKTILSADAGDTVILNLAGRLK
                     NNV"
     gene            complement(1181..1576)
                     /locus_tag="PLI11_09385"
     CDS             complement(1181..1576)
                     /locus_tag="PLI11_09385"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPZ53120.1"
                     /translation="MGEKKRITVTINGRNYTLRHDETDEYVHRIVQYLNNKIADVSRG
                     GLQLGEHIALVMAAINITDELFKTQKNFNTLKNEIKRMMDEYDKLKLEKQELEKQYMQ
                     LSEEYDEVNKKLLIKQIEFNEYRKSVEGR"
     gene            1813..>2268
                     /locus_tag="PLI11_09390"
     CDS             1813..>2268
                     /locus_tag="PLI11_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53121.1"
                     /translation="MPDNKLGSQRANTKGVLPQTKGIRKPGAARRTQSNATSSTALKQ
                     TRIPKAATGEKRQKVKIRKASHKKKVKNRSNLPYLLLFVVFCVCVVFFSSFLSYTYLV
                     DKYNNPTSIESIYIDPETEVKFKIERGATTKDIANNLKEMGLIRNTEVYR"
BASE COUNT          622 a          465 c          444 g          737 t
ORIGIN      
        1 acgttgtttt tcaatctgcc tgcaaggttc agtatgactg tatcccctgc gtccgcagaa
       61 aggattgttt tatggctttc tgtttttatt gaatttacct ttccgccatc ttcagtattt
      121 tctatggcaa taccatcacc tttactgaca ggggatgaaa gttttacctt cagctcgaat
      181 atgtccggag agttcctttt accttttgtc ttgaagattt ttacttttaa aatctcacca
      241 cattcaagcc ctatatggcc tgatgaacta aatgttatat cgacaaaagg catcttctca
      301 aaatgatggt atgacgtaaa tctgcctctt gagaaaatca cggagagatc ttccatgtct
      361 ttttgtgtag ggtattcttc gatttccctg ttttgaataa tatcgagggc ttttctgtac
      421 tttgcggtaa caatgcctgt atactgaggg cttttaagtc ttccctcaat tttcaaggaa
      481 gttatgcctg tattgacaag ttgtgcaaga tagggaagtg tcattaaatc gccaggactt
      541 attctgtagg ctcttttgga aacttccttt ccgtttctta gaaccgaata cataagtctg
      601 cagggctgtg cgcactgtcc cttgttgccg cttcttccac ctatggcgct gctcaacaga
      661 cactgaccgg aatagcttat gcagattgca ccgtggcaga atacttccat ttccattatg
      721 ttttttgagc aaatagtgac tatttcgtca agagaaagtt ctcttgacag aatgcatctt
      781 gaaaacccca aatccttaag gagtcttgct ccctcctcgt tgtatacagt catctgggtg
      841 ctggcgtgca atggagcctt tacatgtttt ttcaacatcc tagccaaacc aagatcctgg
      901 acgatgaatg cgtccacatg cagcatgtcc gccattttag caagttcaat ggcattttca
      961 agatcattgt catcaagaag cgtgtttatg gcgacataaa cgagcacacc tcttggatgt
     1021 gcgtatgata ttgcctgttt aagtttttca atgtcaaagt tatcactgga tgcccttgca
     1081 ctaaatttat taagtccaag ataaacggca tctgcgccgt tttgtactgc gaacttaagg
     1141 ctttcgaaat taccggcagg ggacaataat tctattcttt tcatcttccc tcaacagatt
     1201 ttctgtattc attgaattca atctgcttaa taagtagttt tttattgact tcgtcgtatt
     1261 cctcggaaag ctgcatgtac tgcttttcta gctcttgctt ttcaagtttc agcttgtcgt
     1321 actcatccat catgcgcttt atttcgttct taagcgtatt gaagttcttc tgggttttaa
     1381 aaagctcatc cgttatgttt atggctgcca taacaagcgc aatgtgttcg ccaagctgaa
     1441 gtccgccgcg ggaaacgtcc gcaatcttat tattcaaata ttggacgatt ctatgcacgt
     1501 attcgtctgt ttcgtcgtga cgtagtgtgt aatttctgcc gttaatggtt acagttattc
     1561 tctttttttc ccccatgttg ccaactcctt gcacttatat tgaacctttc acattatgat
     1621 aacattattt gtataatttg tacagacttt ttatatttct cttatgtgtg aaaatcccct
     1681 gattgtggga attcttgacc gatattatgg ggaatggtat aatgaatcgt atctttgatg
     1741 ggtatgatac atacgaaaaa gatatttttc aaagaggatt gcaagagctg atgtatagag
     1801 gtgaaaaagg atatgcctga caacaagctt ggatctcaaa gagccaacac aaaaggcgta
     1861 ttgccgcaaa cgaagggtat cagaaaacct ggtgcagcaa gaaggacgca gtcgaatgca
     1921 acttcatcta ctgcacttaa acagacaaga atacccaaag cagcgacagg cgaaaaaaga
     1981 cagaaagtta aaataaggaa agcttcacat aaaaagaaag tcaaaaacag gagtaatctg
     2041 ccttacctgc ttctgtttgt tgtattctgc gtttgtgtgg tgtttttttc aagctttttg
     2101 tcctacacct atcttgttga caaatacaac aatccaacaa gtatagaaag tatttacatt
     2161 gaccctgaaa ccgaggtcaa gttcaagata gaaagaggag caactacaaa ggatattgcc
     2221 aacaatctca aggaaatggg ccttattaga aacacggaag tgtaccgc
//
LOCUS       DAONVY010000394         2262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32201_length_2262_cov_3.898958, whole genome shotgun sequence.
ACCESSION   DAONVY010000394 DAONVY010000000
VERSION     DAONVY010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32201_length_2262_cov_3.898958"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..714)
                     /locus_tag="PLI11_09395"
     CDS             complement(109..714)
                     /locus_tag="PLI11_09395"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPZ53122.1"
                     /translation="MKDLIHEFNKKYAEYMLSIDGINNGINLEFVFGDGSINSVVMLV
                     GEAPGKDEVALKKPFVGQAGRILSKFLESVNIEREDVYITNAIKYRLSKEGKRPGTFV
                     NRPAKLEEIELAKVFLLEEVDIVKPKIIVPMGNVPLKALFGKNMSIGENHGRIFEGII
                     KDTVVYPIYHPASLIYNRKLEKVYELDILHLRDIINETMHK"
     gene            complement(695..1303)
                     /locus_tag="PLI11_09400"
     CDS             complement(695..1303)
                     /locus_tag="PLI11_09400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPZ53123.1"
                     /translation="MVHKFMKGELAGKAKKYMTWEQLESNCRECRRCGLAYGRTNVVI
                     GRGCKTAPVMIVGEGPGQKEDETGLPFVGRAGELLDLALESLGFSENDYYIANIVKCR
                     PENNRTPTEDECEKCLPYLRAQFALIRPKIIVCLGNVATKAMIDKSFQVSKARGIWYT
                     KNGTLFLATYHPAALLRDESKKIEMWEDLRKVKIRLYEGLDT"
     gene            complement(1348..1839)
                     /locus_tag="PLI11_09405"
     CDS             complement(1348..1839)
                     /locus_tag="PLI11_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HPZ53124.1"
                     /translation="MLVDIGYDRNSAFEYAKKWAYKRNPKYYNFDKLGGDCTNFVSQC
                     ILSGCQVMNYKPTFGWYYINLNDRTPSWSGVIYLYNFLVDNKGTGPYGVEVGLDKIQI
                     GDVIQLGHANGFYHSLFVTGVSSNDIFVSTHTQDAFMRGLSTYFFERFRCIHILGARK
                     YVK"
     gene            complement(1913..>2262)
                     /locus_tag="PLI11_09410"
     CDS             complement(1913..>2262)
                     /locus_tag="PLI11_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPZ53125.1"
                     /translation="WPVQIKLVPVNAPYFNGASLLIAADCTAFAYANFHEKFIKGKIT
                     LIGCPKLDMVDYSEKLTQILRHNNIKNVTVVRMEVPCCGGIVYAVTEALRNSGKMIPW
                     QVVTVSTDGEVIE"
BASE COUNT          637 a          542 c          358 g          725 t
ORIGIN      
        1 gatacgctgt ttagttttca atgtgctgct gtcgttggac agctcgatta gtatagcaaa
       61 cctgaatttg cttgtcaaga gtatattttt aaattaattt ttaccaaatc atttatgcat
      121 tgtttcgttg ataatgtctc gaaggtgtaa aatgtcaagt tcatatactt tttcaagctt
      181 tctgttgtat atcaaactag caggatgata tataggataa acgactgtat cttttataat
      241 tccttcaaaa atcctaccgt gattctcgcc aatgctcatg tttttcccaa acaatgcctt
      301 caatggcaca ttgcccatgg gaacaataat tttaggttta acaatatcaa cctcttcaag
      361 caaaaagact ttggcaagtt cgatctcctc cagtttcgca ggtctgttca caaaagtgcc
      421 tggcctttta ccttccttgc tcaacctgta ctttattgca tttgtaatgt acacgtcttc
      481 cctctcaatg ttgacggact caagaaactt ggacagtatc cttccagcct gtccaacaaa
      541 gggtttcttt aacgcaacct catccttgcc cggcgcctcc ccaaccagca ttacaacgct
      601 gtttatactt ccgtctccaa aaacaaattc caggttgata ccgttgttta tcccgtctat
      661 tgaaagcatg tattctgcat atttcttgtt aaattcatgt atcaagtcct tcataaagcc
      721 ttatcttaac ctttcttaaa tcttcccaca tttctatctt tttgctttca tccctgagaa
      781 gcgcagccgg atgataagtt gccagaaaca gcgtcccatt tttggtgtac cagatacctc
      841 tggcttttga tacctgaaaa ctcttgtcta tcatcgcttt tgttgccacg ttgcccaagc
      901 acacaataat cttaggtctg attaaagcaa actgtgccct taaatacgga agacactttt
      961 cacactcatc ctccgtaggg gttctgttgt tctcaggtct gcacttgacg atgtttgcta
     1021 tgtaataatc attttcggaa aatcccaggg attcaagggc tagatccaac aattctcccg
     1081 ccctgccaac aaaaggcaga cctgtttcgt cctccttttg tccaggacct tctccaacaa
     1141 tcataacagg agcagttttg catccccttc caattaccac attagtcctg ccatatgcaa
     1201 gtccacaacg cctgcattcc ctacaattac tttcaagctg ttcccaagtc atgtatttct
     1261 ttgccttgcc ggcaagttct cctttcataa acttatgtac cattacatta tatcatgaat
     1321 aaagtaagat ttcctacaca aaaccctcta tttcacatac tttcttgcac ccagaatatg
     1381 gatgcacctg aacctttcaa agaaatacgt gcttaatccc ctcatgaacg catcttgggt
     1441 atgagttgaa acgaatatat catttgagga aacacctgtt acaaaaagag agtgataaaa
     1501 accattggca tgacctagct gtataacatc tccaatctgt attttgtcaa gtcccacttc
     1561 caccccatat ggccctgtgc ccttgttgtc aacaaggaaa ttgtacaaat atataactcc
     1621 actccaggat ggagttctgt cattcaaatt gatatagtac cagccaaaag tcggtttgta
     1681 gttcattact tggcagcctg aaagtataca ttgggataca aagtttgtgc aatcccctcc
     1741 aagtttatca aagttataat atttgggatt tcgtttgtac gcccatttct ttgcatactc
     1801 aaaagctgaa tttctatcgt atccaatatc tacaagcata aaaaaactcc taaacacagc
     1861 tttacatata tactatgttc aggagtcttc aggctctaat agttaataag tattattcga
     1921 ttacttctcc gtcagtagaa acagtaacaa cctgccatgg tatcatctta ccgctgttcc
     1981 tcaaagcttc agttactgcg tatacaattc cgccacagca aggaacttcc attctgacaa
     2041 ccgtaacatt cttgatattg ttgtgcctca atatctgagt cagcttctca gaatagtcca
     2101 ccatgtctag tttgggacaa cctataagtg tgattttccc cttaatgaac ttctcatgaa
     2161 agttggcata tgcaaaagca gtacagtctg cagcaatcaa caaactagct ccgttaaaat
     2221 acggtgcatt aactggtacc agctttattt gtacaggcca ct
//
LOCUS       DAONVY010000395         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32311_length_2256_cov_3.264880, whole genome shotgun sequence.
ACCESSION   DAONVY010000395 DAONVY010000000
VERSION     DAONVY010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32311_length_2256_cov_3.264880"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLI11_09415"
     CDS             <1..956
                     /locus_tag="PLI11_09415"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53126.1"
                     /translation="EDYPVISIMIRKRAGTPDSGQIFFHTRSSGGYSQANSITFSYQP
                     TADWQIVSIDMSQNANWKGEVVSIRLDPFGNCNEGDEFKIKYIAFFDSMEKTSIFKGF
                     DKHIVKVEGAENGVVTGGGVYGEGASYNLYASPFEGYAFGGWYDGATGEVISTNPSYQ
                     GVATGNMNITARFFSLEGKNGFVYETLGAQIRTAPPDGLRFVTQVNKSFIPENATNIV
                     YGTLVTLERNLGDRDNTELVIGAEGFKYLDIKGEKLYRETEEYIWFTAVITDIPEHEH
                     DSNFVARSYITYTDGEGTHTIYDRPIVRSVNMVKSMVQGGN"
     gene            complement(1536..2108)
                     /locus_tag="PLI11_09420"
     CDS             complement(1536..2108)
                     /locus_tag="PLI11_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53127.1"
                     /translation="MVYYQLLFYPFLLAVLLPRAQKRWQITFINHAALDPTVFKLLYE
                     ENVLFITEAPQSWGTLKHCYIQSNGIIGLEYTRPIEPPQATPTPDAVNGITPPPQKEK
                     ARTEAFVCFDIRTKENSENQINTITRDCAKEYKLKNETDVYIDSSGNANMIYQGKYYL
                     KLWGDIGSYNEVEQLTLRKLPLDLSRKQNS"
BASE COUNT          689 a          403 c          465 g          699 t
ORIGIN      
        1 cggaagatta tcctgtaata tcaatcatga taaggaaaag agcaggaact cctgattcag
       61 gacaaatatt cttccacaca cgatcatcag gtggctactc acaagctaac tccattacgt
      121 tttcatacca gccaactgcg gattggcaga ttgtgagcat tgacatgtca caaaacgcca
      181 actggaaagg tgaagttgta tccatacgac ttgatccgtt tggtaattgc aatgagggcg
      241 atgagtttaa gatcaagtat attgcattct ttgactcaat ggaaaaaacc agcatattta
      301 aaggttttga caaacatatt gtaaaggttg agggtgcaga aaacggagtt gtaactggcg
      361 gcggagttta tggagaaggt gcttcgtaca acctttacgc atccccgttc gaaggttatg
      421 cttttggtgg atggtatgat ggtgctacag gcgaagttat cagcacaaac ccttcatacc
      481 aaggtgtagc aactggtaat atgaacatta ctgcgaggtt tttcagcctt gaaggaaaga
      541 atggctttgt ttatgagacc cttggagcgc agataagaac agctccaccg gatggattaa
      601 gatttgtaac ccaagtaaac aaaagcttca taccagaaaa tgcaactaat attgtgtatg
      661 gtacacttgt tacccttgag cgcaatcttg gagacaggga caatacggag cttgttattg
      721 gggctgaagg attcaagtat ctggatatta agggtgaaaa gctctacagg gaaacagaag
      781 agtatatctg gtttaccgca gttataactg acatacctga acatgagcat gactcaaact
      841 ttgtagccag aagctatatt acctacaccg atggagaggg tactcacact atatatgata
      901 gacctattgt aagaagtgtg aacatggtca aaagcatggt acaaggaggc aattaagatg
      961 aaattggatt acgagcagcc ggatatagag atatgtttct ttgaaacgga ggatgtgatt
     1021 acagaaagtg gcgataactt cggcagcttc aaggatttga tttagtatca tttacagcag
     1081 gtgggataaa tcccgcctgc taattatttc cccaaaattg actataattc ttatcaggat
     1141 tttaatatgc caacaatagc aagtgatgta aactattact tggttctcga tatttgtgct
     1201 tcggaagctt aatgtatcca tcataaaata tattttccgt tttgattatt tgttgcaact
     1261 tattgcggat actatgaagt gaattttaaa atatacaaca tgtttttgaa aatcataaaa
     1321 cctcttttgc ccactatttt caagggttga cagaggtttt ttcgatttta aactgtaaaa
     1381 ttcaaggtta agtttggtaa tattatatat ataagactgc actctttcaa aatatctttt
     1441 atacactctc tcctttttta cagagggtga tatgcatttc gtgagcccaa caagtcaaaa
     1501 gtacgttgag ttctgtgaga gcaaagaaag cagaactagc tgttctgctt tctagataag
     1561 tccaatggca acttcctaag tgtcagttgt tcaacctcgt tatatgaccc aatgtcgccc
     1621 caaagcttta ggtagtattt tccttggtaa atcatatttg catttccgct agagtcgata
     1681 tatacatctg tttcattttt caacttatac tctttagcac aatctctggt aattgtatta
     1741 atttggtttt cgctattctc tttagtacga atatcgaaac aaacaaatgc ttctgttctt
     1801 gctttctcct tttgaggagg tggggtaatg ccatttacag catctggagt tggtgttgct
     1861 tgaggcggtt caatcggtcg ggtgtattcc aggccaataa ttccattgga ctgtatataa
     1921 cagtgtttga gtgtacccca gctttgtggt gcctctgtta tgaatagtac gttttcctcg
     1981 taaagaagct tgaacactgt tggatccaaa gctgcatgat tgataaatgt tatttgccat
     2041 ctcttctgcg ctcttgggag cagaacggca agaagaaagg gataaaacaa taattggtaa
     2101 taaaccaaat aatactatcc ttttcatata ttattacctc ctaaaccagg aactgcaaat
     2161 cttgaatact caggaaaacc atagagcttt aacgttactg ccaattttaa tcattgccta
     2221 atgctaaggt tttaccccta agaagttctc ttaaag
//
LOCUS       DAONVY010000396         2248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32440_length_2248_cov_3.497492, whole genome shotgun sequence.
ACCESSION   DAONVY010000396 DAONVY010000000
VERSION     DAONVY010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32440_length_2248_cov_3.497492"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLI11_09425"
     CDS             <1..731
                     /locus_tag="PLI11_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPZ53128.1"
                     /translation="IPVGTLTAILNIPLFIAGYKVLGKSFIFRSFVGTILFSIMIDVL
                     SVINWIGISDKIGLTTDGVVNDFILSSIWGGVLYGMGLGLIFRAGFTTGGTDIAARLL
                     QRKFSWLSLGQLLLAMDILILTLVAITYEKPVFALYAGITIFLTSKLIDVVEAGLHYA
                     KEVYVMTNKAEDIAKDVMTELDRGTTKMAGTGMYTGAHIDILICVIYNRQLTALRRII
                     DRHDPDAFIIIKDVREVEGLYSDL"
     gene            744..1517
                     /locus_tag="PLI11_09430"
     CDS             744..1517
                     /locus_tag="PLI11_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPZ53129.1"
                     /translation="MFVDTHAHYDDSAFDEDRHETLMRNKEYVHFIVNAAQDLKTTDA
                     CIELANTYNFVYATCGIHPHNANSFSEEVMEQLYKRALDDNVVAIGETGLDFHYDFSP
                     REIQVKSFRANINLAREVKKPVVIHSREATEETLRILKEERVNDIGGVLHCFTGSFET
                     ALEANKLDLYYSIGGVVTFKNAKKIVKALEAIPNDRLMLETDAPYMTPVPYRGRRNES
                     MYLSYVARKIAEIKGMDYEELVELTNINAKRFFVYSAQA"
     gene            1645..>2248
                     /locus_tag="PLI11_09435"
     CDS             1645..>2248
                     /locus_tag="PLI11_09435"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HPZ53130.1"
                     /translation="MKKGKYAKLFALVRYPVMLIGVTVMSIALASGIFASNINTVTIC
                     YNGTLIDVTTTGRTVKEALSVAGINLTEDDYINYDIDTSLSGVDFIEIETPITLTVNV
                     EGREVMIKSRQSTVSEVLEQNDIELTARDLVIGAELDEITENGMNLSIVRIQEEYDIE
                     NITIEYETEYVPSNYLLKGQTKVLTPGREGVRTLVYRVIKE"
BASE COUNT          710 a          396 c          534 g          608 t
ORIGIN      
        1 tgattccagt aggtacgttg accgccattc ttaacatccc cttgttcatt gcagggtaca
       61 aggttctagg aaaatcattc atattcagaa gttttgttgg aacgattctt ttttccatca
      121 tgatagacgt actttccgta ataaactgga taggcatttc agacaaaatc ggattaacca
      181 ccgatggtgt cgtaaatgat tttatccttt cgagtatttg gggtggtgtt ttgtacggca
      241 tgggacttgg tctcatcttc agagcaggtt ttacgacagg aggaaccgac attgcagcaa
      301 gacttctgca gagaaagttt tcatggctgt cattgggaca gctgcttctt gcaatggaca
      361 tattaatcct tacactggtg gccataacgt atgaaaaacc tgtttttgcc ctttatgcgg
      421 gaataaccat cttcctaact tcaaaactta ttgatgtggt ggaagcaggt ctccactatg
      481 cgaaagaagt atatgtgatg acaaacaagg cggaagacat tgcaaaagac gtaatgacgg
      541 agcttgatag ggggactaca aagatggctg gaaccggcat gtatacaggt gcgcatatcg
      601 atatattgat ttgcgtgata tataaccggc agcttacggc tttgagaagg ataatagacc
      661 gacatgaccc ggatgcattc attataatca aggatgtaag ggaagttgaa ggattatact
      721 cggatctgtg aaaggatgta aaaatgtttg tagacactca cgcacattat gatgacagcg
      781 cttttgacga ggaccggcat gaaactttaa tgcgtaataa ggagtacgtc cattttatcg
      841 taaatgcagc ccaggatttg aagactacgg atgcatgcat agagcttgca aatacgtaca
      901 acttcgtata tgcaacctgc ggcattcatc cgcataatgc aaacagcttc tcggaggagg
      961 ttatggagca gctttacaaa agggcgcttg atgataatgt ggtggcaatc ggggagacag
     1021 gccttgattt ccactatgat ttttccccga gagaaattca ggttaagagt tttagggcaa
     1081 atatcaatct ggccagggaa gttaaaaagc cggttgttat ccattccagg gaagcaactg
     1141 aagagactct cagaattctc aaggaagaaa gagtcaatga tattggaggg gttctgcact
     1201 gctttaccgg aagtttcgag actgcacttg aggcaaacaa gcttgattta tactattcga
     1261 taggtggagt tgtaacgttc aagaatgcca aaaagattgt aaaggcattg gaagccatac
     1321 caaatgacag gcttatgctg gaaacggacg caccatatat gactcccgta ccgtacagag
     1381 gaagaagaaa cgagtccatg tacctttcat atgttgcaag aaaaattgct gagataaaag
     1441 gaatggatta cgaagaactt gttgaactga caaatatcaa tgcaaagcgt ttttttgtgt
     1501 atagtgcaca agcgtaactg gaatatttta tgaaatgttg tgcacagtac acaaagtttt
     1561 tactaaaaag ttgacacttt gcataagata taatagaatc tgacaggtgt ttagctgtaa
     1621 acatacttaa caggagttga tactttgaag aaaggtaaat atgctaaact gtttgcgttg
     1681 gttagatatc ctgtaatgct tattggcgtg acagtaatgt ccatagcttt ggcgtccggg
     1741 atcttcgcca gcaacataaa cacggttacc atatgttaca acggaacact tattgatgtt
     1801 acgaccaccg gtagaaccgt aaaggaggcc ttatcggtag caggtattaa tcttacagaa
     1861 gacgactaca taaattatga tatagataca tccctttccg gagtcgattt cattgagatc
     1921 gaaaccccaa ttacacttac tgtaaatgtt gaaggcaggg aagtaatgat taaatcccga
     1981 cagtcaacag tttcagaagt gttggaacag aacgatatag agcttactgc ccgggatttg
     2041 gtaataggag cggagctgga tgagataacc gaaaatggga tgaatctatc catcgtaagg
     2101 atacaggaag aatatgatat tgagaacata acaattgaat atgagacgga atacgttccg
     2161 tcaaactatc tgctgaaggg tcagactaag gtacttaccc cgggtaggga aggtgtaaga
     2221 accttggtat accgtgtaat caaagaag
//
LOCUS       DAONVY010000397         2246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32479_length_2246_cov_2.713373, whole genome shotgun sequence.
ACCESSION   DAONVY010000397 DAONVY010000000
VERSION     DAONVY010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32479_length_2246_cov_2.713373"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..878
                     /locus_tag="PLI11_09440"
     CDS             123..878
                     /locus_tag="PLI11_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPZ53131.1"
                     /translation="MKLSMMERQKRTLFLVLFAVILAIEAIVCFTPLGSLPALGPIVA
                     TLAMIPVTIAGILMGPIAGALMGFFAGTFSFIVWTFMPPPLSIAFAFVFTPFNLVGEN
                     AGSIWSLVICFVPRIMTGLMAGFLYTIFRKRKLFTKSPFLLNFLSGFFASLVNTFGVL
                     SGIFLFFRQPYASVLEKTYALLLITLLTTVLTSGIPEAVLAGLASHAVCHPTKKHIFK
                     GDVSVFSANTEYTVSVVAITLTIVLYVVGAILN"
     gene            complement(998..1816)
                     /locus_tag="PLI11_09445"
     CDS             complement(998..1816)
                     /locus_tag="PLI11_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPZ53132.1"
                     /translation="MNKKLGILLVILFLILCIILIMKLEEDREHQSAPTQTIHTSSPY
                     ATSTETPEPTLEPTLSPTPTPEATPEPRLPEGFVYVKDVIPYIHLDVRYYTDKNFIGS
                     RIDGYNAPVAILTVEAAEALKKACEIFYQKGYIVTIYDAYRPQKAVEHFVRWSEDESD
                     TRMKEIYYPNLHKSYLIGNFISSKSGHSRGSTVDMSLLDLEGKEVDMGGYFDLFDSLS
                     YYENSKITDIQRENRKLLREVMVECGFIPSNGEWWHFRLDNEPYPDTYFDFDVE"
     gene            complement(1827..>2246)
                     /locus_tag="PLI11_09450"
     CDS             complement(1827..>2246)
                     /locus_tag="PLI11_09450"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HPZ53133.1"
                     /translation="FLDLVPTILDVCSIGKPEDATLHGRSLRPIIMDEGIPKDWRQEA
                     VSSYNGQQFGLFCQRSIKTDRWKYVWNPTDIDEVYDLENDPGETRNLVNEVEIDILSD
                     LRKRLYETLAKDEDWLVMRNNHSWVAPQLLHNKKHAR"
BASE COUNT          627 a          481 c          390 g          748 t
ORIGIN      
        1 atcacaagaa tttaaagaat tttaaaagca aaaagtaata acaatacgct gtataaaata
       61 cagctcccat attaacatat acgctgtatt tacataataa aacagcagtt aggaggaaaa
      121 ctatgaaatt atcaatgatg gaaagacaaa agcgcacact ttttttggtg ctatttgctg
      181 taatattggc aattgaagcg attgtatgct tcacgccact aggatcttta cccgctttag
      241 gtcccatcgt ggctactctt gcaatgattc ctgtcactat tgccggtata cttatgggcc
      301 ctattgcagg tgcattaatg ggatttttcg caggaacatt cagttttatt gtctggacat
      361 tcatgccccc accattaagc attgcatttg cttttgtgtt tacacctttc aacctcgtag
      421 gtgaaaatgc aggaagcata tggagtcttg ttatttgctt tgtacccaga attatgactg
      481 gtttgatggc aggttttcta tacacgatat tcaggaaacg caaactcttt acaaaaagtc
      541 cattcctgct caacttcctt agcggttttt ttgcaagtct ggtcaatacc tttggagttc
      601 tctcgggaat attcctgttc ttcagacagc catatgcatc cgtacttgaa aaaacttatg
      661 cactattatt aatcactctg ttaactacag tcctcacgag cggtattccc gaagcggttc
      721 ttgcaggtct tgcatcccat gcggtatgcc atcctaccaa gaaacacatt ttcaagggtg
      781 atgtaagcgt tttctctgca aatacggaat acacggtttc ggttgtagca ataaccttga
      841 caattgttct ttatgtagtt ggagcgatat taaactaaag gaaagtagtc cctccttaat
      901 atatgaaaaa agaaggccat gccttctttt tttatgtttc tgccattctt ctttttattg
      961 cctacttctt tgaaacactg ttagaaaacc atcatgtcta ctccacatca aagtcaaagt
     1021 atgtgtcagg atatggttca ttatccaacc taaagtgcca ccattcgcca tttgaaggaa
     1081 taaatccaca ttccaccatt acctctctta aaagttttct gttttctctt tggatatcgg
     1141 ttatctttga gttttcataa tatgagagac tgtcaaacaa atcaaaatat cctcccatat
     1201 ctacctcttt accttctaga tcaagaagag acatatcaac agtacttcct ctggagtgac
     1261 ctgattttga tgatataaag ttgccaataa ggtaggattt atgcaaatta ggataatata
     1321 tttctttcat ccttgtgtcg ctttcgtctt cagaccacct gacaaaatgc tcaacagcct
     1381 tttgaggtct gtatgcatca tatatagtta caatatatcc tttttgatag aaaatctcgc
     1441 aggccttttt caatgcttct gccgcttcta ctgtaagaat tgcaacagga gcattgtagc
     1501 catcaatcct gcttcctata aaattcttat cagtgtaata cctaacatcc agatgtatat
     1561 aaggaattac atcctttaca tacacaaaac cttcaggcag cctgggttct ggagttgctt
     1621 ctggagttgg agtcggactt aatgtaggtt ccaatgtcgg ttcaggtgtt tctgtagagg
     1681 tggcgtacgg acttgaagta tgaatcgtct gtgtaggggc agattgatgt tccctgtcct
     1741 cttcaagttt cataatcagg ataatacata gtattaagaa taatatgact aataaaatcc
     1801 caagcttttt attcatacct tgtccttcat cttgcatgct ttttgttatg caaaagctgc
     1861 ggagccaccc agctatgatt attcctcata accagccagt cttcatcctt tgcaagcgtt
     1921 tcataaagtc tctttctaag atctgacaat atgtcaattt ccacttcgtt tacgagattc
     1981 ctcgtttcac ctggatcatt ctcaagatca tacacttcat caatatccgt tggattccac
     2041 acatatttcc acctgtctgt cttgattgac ctttggcaaa acagaccgaa ctgttgcccg
     2101 ttatacgagc ttacagcttc ctgcctccag tcttttggaa tcccttcatc cattattatt
     2161 ggacgaaggc ttcttccatg cagtgtggca tcttcaggtt ttcctatact gcatacatca
     2221 agaattgttg gaacaaggtc aagaaa
//
LOCUS       DAONVY010000398         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32522_length_2243_cov_2.911335, whole genome shotgun sequence.
ACCESSION   DAONVY010000398 DAONVY010000000
VERSION     DAONVY010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32522_length_2243_cov_2.911335"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLI11_09455"
     CDS             <1..555
                     /locus_tag="PLI11_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53134.1"
                     /translation="NLLDEIGFDYCALGHIHKPIANAAYTFPGTPEGRGFDEIGELGI
                     VVGYIEKGDVNTKFYPISKKRYVIKELDITNLISDDAILSRINEECTSSKDHYRIILK
                     GTVKTSPDTKVLESVLSPYYAHMEIIDETVMSIDYERLKKENTLKGIFLRNMLDKMRN
                     EPDNRILAESLMLGLKAFDEDIEV"
     gene            559..>2243
                     /locus_tag="PLI11_09460"
     CDS             559..>2243
                     /locus_tag="PLI11_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ53135.1"
                     /translation="MYISKIRIYGFGRLKNLEMDFSKDINIIYGPNESGKSTILAFIK
                     ACLYGLSSRGNLSERTKYAPWDNPQSFGGEIFFEHENRKYRYICTFGKSKRNNEYTLY
                     NETTGEKVTIPSGMSLGEYIFNLSEGAFDSSIFAPQLQSKIDASRDKDGQIMSLIAKS
                     QVGEEESINIVERRLKNAMDTIKAPRSGKGILDKLYALKKENDDAFNKIKEEEDHLKM
                     IKIELESLVAEKEEIDERINFFYAKERIRHRNMVFQQMSKIDHLQEAMQKTSEKRAKP
                     SSKIYYISTLLLLVLSGVLYFLPIPTPFAFIPILFVMVLSVLASRQKNEVNKAAMTMM
                     ELKKETELLEHMLQGKDLSEHEKDWKKAEEVLRNSKGFEEESINVLVKRQEELTKMIA
                     FKNAQIQTAKSAFDYASLLEEKKHLKEKTNYYEDKYKALEIAYETVRDSYSQLQNLFG
                     PKIADETGKILKVLTNSTHDNIRISKDFDISVDEETGYFPHQFYSGGTIDQVYLAFRL
                     AVSKIIAPYGALPLYLDDPFVQYDDERLESAMYCIKKHNSIHNNQIILTACHK"
BASE COUNT          815 a          398 c          464 g          566 t
ORIGIN      
        1 aatcttcttg atgaaattgg gttcgactac tgtgctttgg gacacatcca caaacccatc
       61 gcaaacgctg catacacctt tcccggcact cctgaaggaa gaggctttga tgagattgga
      121 gaattgggga tagttgtagg atacattgaa aaaggagatg taaacactaa attttatcca
      181 ataagcaaaa aaagatatgt cataaaggaa ctggacataa caaacctgat tagtgatgac
      241 gcaatactaa gtagaataaa tgaagaatgt acaagctcaa aagaccatta ccggataatc
      301 ttgaaaggca ccgtgaaaac aagtccagac actaaggttt tggagtctgt actgtctcca
      361 tattacgctc atatggaaat tatcgatgaa acagttatga gtattgacta tgaaaggctc
      421 aaaaaagaaa acacattaaa aggcatcttt ttaagaaaca tgctggacaa aatgagaaat
      481 gaaccggaca acaggatcct tgctgaaagc ttgatgcttg gactgaaggc tttcgatgaa
      541 gatattgagg tgtagaacat gtatatatca aagataagga tatatggttt tggtagatta
      601 aagaatcttg agatggattt ctcaaaggac ataaatatta tttacgggcc taatgaaagt
      661 ggcaaatcca caatcctggc attcatcaaa gcctgcttgt atggcctttc ttcaagaggc
      721 aatttaagcg aaagaacaaa gtatgcgcct tgggacaacc ctcaaagttt tgggggagag
      781 attttctttg agcatgagaa tagaaaatac agatacatat gcacttttgg caaatcaaag
      841 cgtaataatg agtatacatt atacaacgaa accacaggtg aaaaggttac aattccaagt
      901 ggcatgtctt tgggagaata cattttcaat ctttccgaag gcgcttttga ttcctctatt
      961 ttcgcacccc agctgcaatc aaaaatcgat gcatccaggg acaaagatgg tcagataatg
     1021 tcattgatcg caaagtcgca agttggcgag gaagaatcca tcaatattgt tgaaagaaga
     1081 cttaaaaatg caatggacac cataaaagcc cccagatccg gaaaaggcat tttggacaag
     1141 ctgtatgcac taaaaaaaga aaacgatgat gcttttaaca aaataaagga ggaagaggac
     1201 cacctcaaga tgataaagat agagcttgaa agtcttgttg cagaaaaaga ggaaattgac
     1261 gagagaataa attttttcta cgccaaggaa agaataaggc atagaaacat ggtgtttcag
     1321 cagatgtcga aaattgacca cctgcaggaa gccatgcaga aaacttccga aaaaagggca
     1381 aaaccatcct ccaagatcta ctacatctca acccttcttt tattggttct ttccggagta
     1441 ctgtactttt tacccattcc cactcctttt gcctttatac ccattctttt tgtaatggtt
     1501 ttgtcagtac ttgcctcaag acaaaagaac gaggtcaaca aggcagccat gactatgatg
     1561 gagctaaaaa aggaaacaga gttgcttgag cacatgcttc aaggaaaaga tctgtcagaa
     1621 catgaaaagg attggaagaa ggcagaagaa gtattaagga attcaaaagg ctttgaagaa
     1681 gagagcatta atgtgctggt aaaaaggcag gaagaactaa ccaaaatgat tgcttttaaa
     1741 aacgcccaaa tacagaccgc aaagagtgcg tttgattatg cttctcttct tgaagaaaag
     1801 aaacacctga aagagaaaac aaattactat gaggataaat ataaagcttt ggaaatagct
     1861 tatgagaccg taagggattc atacagtcag ctgcaaaacc ttttcggtcc gaaaatagct
     1921 gatgaaacag gcaaaatatt aaaagttctt acaaacagca ctcatgacaa tataaggatt
     1981 tcgaaagatt ttgacatatc cgtggatgag gaaacaggct acttccctca ccagttctac
     2041 agcggaggta ccattgacca ggtatatctt gctttcaggc ttgctgtaag taaaattatt
     2101 gcaccatatg gagcacttcc tttgtacctg gatgaccctt ttgtgcagta cgacgatgag
     2161 agactagaaa gtgcaatgta ttgcataaaa aaacacaaca gcattcacaa caatcagatt
     2221 attcttactg cttgtcataa aag
//
LOCUS       DAONVY010000399         2242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32538_length_2242_cov_3.596251, whole genome shotgun sequence.
ACCESSION   DAONVY010000399 DAONVY010000000
VERSION     DAONVY010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32538_length_2242_cov_3.596251"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1361
                     /locus_tag="PLI11_09465"
     CDS             159..1361
                     /locus_tag="PLI11_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53136.1"
                     /translation="MVKVMQFRNYVTYKDGVYTIKDNFEILLDKEMEFNKFSIYYDST
                     GYIKGKVTYMLNKEYEEVFFLEPGKDMAFSSLIDGFFAGKTAKSIKKITLTVLKDVSC
                     EFKLKNVEILKVKVPKTTVFIENSRYKVGVDLLWGGSLNYIEDKKNKDESITNLLNRH
                     DTGRLVQQAYYGTGEKPYEPGTYNGARWPYNPVQGGDMYGNESKIVDFKIEEDRIYIK
                     CRPRDWAKQAEYTICYMENEYSIEKECIKVSNRFVDFSGYKAKPRHQEMPAFYVISYL
                     DTFVFYNGNNPWTNDALTYKENLPFWANNKDAYFRVKEGNTEVWCAWVDKDHYGIGLY
                     AADTEILLAGIYMADRSKSSYAPSTSYVAPLRTFELKSFEPFEYSYIIAAGKVDEMRA
                     IFNKHYVT"
     gene            1396..2223
                     /locus_tag="PLI11_09470"
     CDS             1396..2223
                     /locus_tag="PLI11_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53137.1"
                     /translation="MVLDVVMKYDGAAPVGFNVNFMGKTKVVTENNPKASFKFEKKGT
                     FELEIVQIPKEEELTKFKKILNILLLPVIGLIKIFSSFGDAKLLKKLNPYLVSQKIAV
                     DLDGDTEIGFVYQKAEYDKKQKAWVKPSLQFPSLMNIERYEVAQNVHDFMRCYTDFRC
                     SSIAVCFDVVLLFMIIAFIALSKSSMMVFTSFLVLSLISILICVLAMVFVKKRMDGMK
                     EEFLKRADLVKDFRFVCETEIFPLYLKYGCLIHLLFILRKFTELYTFSFLVFVAYCN"
BASE COUNT          725 a          311 c          522 g          684 t
ORIGIN      
        1 cttgcaaaac tggcaaggaa gcatttgaat tacgtttatg aggggaattg ttgagtttgg
       61 gagtgtggag attgagatat ctgagcctaa tgttgatacc attgtgtttt gttttggttt
      121 tttgtgatat gggtgacaag gatgtattgg ggaggagtat ggtcaaagtt atgcagttta
      181 gaaattacgt gacttataaa gatggtgtat acacaattaa agataatttt gagattctgc
      241 ttgacaagga aatggagttt aacaagttca gtatatatta cgattccaca gggtacataa
      301 aaggaaaagt aacgtatatg ttgaataagg aatacgaaga agtttttttt cttgaacctg
      361 gcaaggatat ggctttctca tccttgattg acggattctt tgcggggaaa acggctaaga
      421 gtataaaaaa gataactttg acagtcttaa aggacgtatc ttgcgagttt aagttgaaga
      481 atgtcgaaat attaaaggta aaggtaccta aaaccaccgt atttattgag aacagtcgct
      541 acaaggttgg tgttgatttg ctttggggtg gaagtcttaa ttatattgaa gacaaaaaga
      601 acaaggatga gtcaataacg aatcttctaa acaggcatga cacaggcagg ctggttcagc
      661 aggcatatta cggaacagga gaaaaaccat atgaaccagg aacgtacaat ggagcgcgct
      721 ggccatacaa tccggttcag ggaggagaca tgtatggcaa tgagagcaag atagttgatt
      781 tcaagattga agaagacaga atatacataa aatgcagacc cagggactgg gcaaagcagg
      841 cagagtatac aatatgttat atggaaaatg agtattccat tgaaaaagaa tgtataaagg
      901 tatccaacag gtttgttgac ttctcaggat acaaggcaaa gccaagacat caggaaatgc
      961 ctgcgttcta tgtaatcagt tatcttgaca cctttgtctt ttacaatgga aacaatcctt
     1021 ggacaaacga tgcgctgaca tacaaagaaa atctaccttt ctgggctaac aacaaggatg
     1081 catatttcag agtcaaggaa ggtaatacag aagtatggtg tgcatgggtt gataaagatc
     1141 actacggtat aggtctttat gctgccgata cggaaatatt gcttgcaggc atttatatgg
     1201 cggacaggag caagagctct tacgctccat caaccagcta tgttgctccc ttgagaacct
     1261 ttgagcttaa atcttttgaa ccttttgaat acagctatat catagcagca ggaaaagttg
     1321 atgagatgag ggcaatattc aataaacatt atgtgacata aaagattagc aaatccagga
     1381 aaggtggtaa ttgtcgtggt acttgatgta gtaatgaaat atgatggtgc agcaccggtt
     1441 ggattcaatg tgaattttat gggtaaaacc aaggttgtta cagagaataa tccaaaggcg
     1501 tctttcaagt ttgaaaaaaa gggcaccttt gaactggaga ttgttcagat tcccaaggag
     1561 gaagaattga caaagtttaa gaagatattg aatatactgc ttcttcctgt tattgggtta
     1621 ataaaaattt tttcttcctt tggagatgcg aaactgctga agaaactaaa tccgtacctt
     1681 gtttctcaga aaatagctgt ggatttagat ggggatacgg agattggctt tgtgtaccag
     1741 aaggctgagt atgacaaaaa gcagaaagca tgggtaaaac caagccttca gtttccaagt
     1801 cttatgaaca tcgaaaggta cgaggttgca cagaatgtgc atgattttat gaggtgctat
     1861 acagatttta gatgttcaag tatagccgta tgctttgatg ttgtattgct ttttatgatt
     1921 attgctttta ttgcattaag taaaagcagt atgatggtat ttacttcatt tttggttctt
     1981 tccttgatct cgattctaat atgtgttttg gcaatggttt ttgttaagaa aagaatggat
     2041 ggaatgaaag aagagttttt gaaaagggct gaccttgtga aagatttccg tttcgtgtgt
     2101 gagacggaaa tctttccttt atatcttaaa tacggatgct tgatccattt gttatttatt
     2161 ctcagaaagt ttacagagct gtatacattt tcttttcttg tatttgttgc atattgtaac
     2221 tgacatatgt ataataatat ga
//
LOCUS       DAONVY010000400         2238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32612_length_2238_cov_3.966560, whole genome shotgun sequence.
ACCESSION   DAONVY010000400 DAONVY010000000
VERSION     DAONVY010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32612_length_2238_cov_3.966560"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLI11_09475"
     CDS             complement(<1..1275)
                     /locus_tag="PLI11_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPZ53138.1"
                     /translation="MDRFSNAIINHKRIVFYLFIIAALVCAFLAMFVSVNYNMVDYLP
                     KDAQSTVAIRIMEDEFENDFPNARVMIQNVSVMEAFEYKKKLSSISGVQSVTWLDDVT
                     GIDILKTTPLEFLDNAIVENYYKNKSALYTLTIESGKELDALNEIYKVIGKDNAVSGD
                     AVSLAKTQEMSVTEVLKALGILLPIIIVILVVSTTSWIEPVLFLSAIGAAVLTNMGTN
                     LIFKEVSFITLTVSPILQLAVSMDYAIFLLHSFEHHRRDHEPKQAMALAMKQAVPTVA
                     ASAITTVIGFAALLFMRFGVGSDLGLNLFKGVLLSFISVMVFLPALTLMLHKLIDKTK
                     HRKLIPSVNKAGNLLMKLRIPSLIIALLIVIPCFLGQSETKFLYGTGSIANVDRIKRD
                     TTLINKEFGDENIFSLLVPKENPGKESELASEL"
     gene            1446..2030
                     /locus_tag="PLI11_09480"
     CDS             1446..2030
                     /locus_tag="PLI11_09480"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HPZ53139.1"
                     /translation="MKKVKLDRRVRYTRMVIKESFINLLEKKDISQITIKEICKDADI
                     NRSTFYSHYTDQYDLMRKIQDELLDNIRDYLAELEKSEDEVDSVLLAEKIFEYIKQNA
                     KICKILLSEKGGLNFQKSVMTLVYDTIINELTDNSNITKEDAEYVYSFTITGCVGIVQ
                     KWLDDDMKKSARFMAETVITLTMGLIGLLKNERK"
     gene            2041..2178
                     /locus_tag="PLI11_09485"
     CDS             2041..2178
                     /locus_tag="PLI11_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53140.1"
                     /translation="MNIEFVNPGADHMVESILGFQTEEATSFWTDPLFWFYPQLDKEH
                     F"
BASE COUNT          699 a          408 c          456 g          675 t
ORIGIN      
        1 aagttcgctt gcgagttctg actctttgcc cggattttct tttggtacga gcaaggaaaa
       61 aatgttttca tcgccaaatt ctttgttgat aagggttgta tctcttttta tgcggtcaac
      121 attagctata cttcccgttc cataaaggaa ttttgtttct gactggccta agaagcaagg
      181 tattactata agtagggcaa ttattaatga aggtattcta agcttcatta agagattgcc
      241 tgccttgtta acacttggta tcagctttct gtgcttggtt ttatctataa gtttatggag
      301 cattagtgtc aaggcaggca aaaacaccat aactgagatg aagcttaata acacaccttt
      361 aaaaaggttg agccctaaat ccgaaccaac cccaaaacgc ataaatagca gtgctgcaaa
      421 accaatgaca gttgttattg cgcttgctgc aacggttggt actgcctgct tcattgcaag
      481 agccattgct tgtttgggtt cgtgatctct tcggtgatgt tcaaaggagt gaagcagaaa
      541 gattgcatag tccatggata ccgccagctg taatatgggg cttacggtta aggttataaa
      601 tgagacttct ttaaatatga ggtttgtacc catgttggtt agaactgctg cacctatggc
      661 ggacagaaac aaaacaggtt caatccatga tgtggttgat acaacaagaa ttacaattat
      721 tatcggcaaa agtattccaa gagcctttaa tacttctgtt accgacatct cctgagtttt
      781 ggcaagactg accgcatcac ccgatactgc gttatccttc cctatgacct tatatatttc
      841 attcagtgca tccagttctt tgccgctttc tatggttaat gtgtagagtg cgcttttgtt
      901 cttgtagtag ttctcaacta ttgcattgtc caaaaactca agaggagtgg tttttaatat
      961 gtcgatacct gttacatcat ccagccaggt gacagattga accccgctta tggaagatag
     1021 ctttttcttg tactcaaaag cttccatgac agaaacattt tgtatcatga ctcttgcgtt
     1081 tggaaaatca ttctcaaact cgtcctccat tatcctaatc gcaacagtgg attgcgcatc
     1141 cttcgggaga tagtctacca tgttgtaatt gacagataca aacatggcca ggaatgcgca
     1201 aaccagtgct gcaattataa atagatagaa aacaattctt ttgtgattga taatagcatt
     1261 cgagaatctg tccatatcct ctcacctttg ttaaagcaca caatgttgac aaaacattat
     1321 atatacagtt taataacatt atggataaaa ctcaacagac agatgcttat tatatgttag
     1381 ttgtcaaaca tatgagtata atgtgattga tatacaacaa aattcagcaa attgaggtgt
     1441 ggtgaatgaa gaaagtaaag cttgatagaa gagtaaggta tacaagaatg gttattaaag
     1501 aaagcttcat aaaccttctt gaaaagaaag atatctccca gattaccatc aaggaaattt
     1561 gcaaggatgc tgatattaat cgttccacct tctattccca ctataccgac cagtatgacc
     1621 tgatgagaaa gattcaggat gaacttcttg acaacataag ggactatttg gcggaacttg
     1681 aaaagagcga ggatgaagtt gattcagtat tgctggcaga gaagattttt gagtacataa
     1741 aacagaatgc caaaatatgc aagatactcc ttagcgaaaa aggtggtctt aactttcaga
     1801 aaagtgtaat gacccttgta tatgacacca tcataaacga actgacagac aacagcaaca
     1861 ttacaaaaga ggatgcggag tacgtatact cctttaccat aacaggatgt gtgggaattg
     1921 ttcaaaagtg gctagacgat gatatgaaaa aatcagcccg cttcatggct gaaacagtta
     1981 taacgctcac catgggactt attggtcttt tgaagaatga gaggaaatag agaggttgta
     2041 atgaatattg aatttgtaaa tcctggtgct gaccacatgg ttgaaagtat tcttgggttt
     2101 cagacagaag aagcaacttc cttctggacg gaccctcttt tttggtttta tcctcagctg
     2161 gataaagagc atttttgagt ttttttacag aaaacttagc cgaaagccca cgactttagt
     2221 cgttgggatg aaggttta
//
LOCUS       DAONVY010000401         2238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32613_length_2238_cov_3.417774, whole genome shotgun sequence.
ACCESSION   DAONVY010000401 DAONVY010000000
VERSION     DAONVY010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32613_length_2238_cov_3.417774"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLI11_09490"
     CDS             <1..776
                     /locus_tag="PLI11_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53141.1"
                     /translation="NQPVIIESNTRYLESKNKKEVTYILANISEDGSSGDLMYMSNYK
                     AREISNNVNRIIYNSIDDKLVVFAKDKGKMVEIYKSKRGSAPELLLETTHGSRFIYDR
                     IQGYLYINEQTGNLLRVNVYDKSHKVEKVADNAANIYDYLNKAIVMYEDEGADNLYYI
                     LENNKIDRVPSDSKRLYGFSDDAYLFVRSTSSGKVTLDIVTDEGLKRITGDLKGSLFF
                     NKNFEEIIFQEGNKLYLWKKDKLHEIGEYSRINSVAIME"
     gene            789..1820
                     /locus_tag="PLI11_09495"
     CDS             789..1820
                     /locus_tag="PLI11_09495"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPZ53142.1"
                     /translation="MKNFIIFMIIILMLIVVVLVIPKKDDNLVPVTPTPTISNTPQPT
                     PNLDATPTPPELDKEIEFTDVYLELVVRELMNKPIGAILESEVRQIKELEARVCAIRS
                     IEDLKYFTSLERLDLYGNRISDISVLKHLTNLKYLDLSHNFSSIYVGDREGMVLEPLR
                     NLTRLEVLYLDKNFISDISPLSSLVSLKRLSLKQNRISDVSPLKRCVNLEYLDLSKNY
                     HEASEQEYYGVEDLTELANLQKLVYIDISFNTVEDITPLSVLSNLEVLLAGDNWIEDI
                     ECLRGHEKLRELDVHLNMISYFDAILEMPSLKKLFYDRNPIMDYYPIDLFEGVITPTP
                     DLSTPQPTY"
     gene            1914..>2238
                     /locus_tag="PLI11_09500"
     CDS             1914..>2238
                     /locus_tag="PLI11_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPZ53143.1"
                     /translation="MLIEKIRKNSDEYGSIPFWSWNDKLDPEELRRQINVMKKIGMNG
                     FFMHARGGLETDYLSDDWYDCIRQCVDEAKKLGMEAWSYDENGWPSGFAGGKLLEDKK
                     NHATYL"
BASE COUNT          758 a          366 c          508 g          606 t
ORIGIN      
        1 ccaaccagcc cgttattatc gaaagcaata caaggtatct tgaatcaaaa aacaagaagg
       61 aagtaacata cattcttgca aacatttccg aggatggaag cagcggagat cttatgtata
      121 tgagtaacta taaggcaaga gaaatatcaa ataatgttaa caggataata tacaactcca
      181 ttgatgacaa acttgttgtt tttgcaaaag ataaaggaaa gatggtggag atatacaagt
      241 caaagagagg ctccgcacct gaacttttgt tggaaacaac tcatggtagc agattcatat
      301 atgacagaat acaaggatat ttgtatataa acgagcagac aggaaacctt ttaagggtaa
      361 atgtatatga caagagtcac aaggtggaaa aggttgctga caatgctgcc aacatttacg
      421 attacctcaa caaggcaatt gttatgtatg aggacgaggg ggctgacaac ctctattaca
      481 ttcttgagaa caataagata gatagagtgc cgtcagacag caaaaggctg tacggattta
      541 gcgatgatgc gtatctgttt gtaagaagca catcttcagg aaaggtgacc cttgatattg
      601 ttactgatga aggattgaag agaataaccg gtgatttgaa aggaagtctg tttttcaata
      661 aaaactttga ggaaatcata tttcaggaag gcaacaagtt gtatttgtgg aaaaaagaca
      721 agctgcatga aataggggag tacagcagga ttaatagcgt tgctatcatg gaataagggg
      781 tggtaggttt gaagaacttt attattttca tgataatcat acttatgctg atagtagtgg
      841 ttttggttat tccgaagaag gacgataacc tggtgcctgt tactccaaca ccaaccatat
      901 ccaatacacc ccagccaact ccaaacctgg atgcaactcc aactccacct gaactcgata
      961 aggaaattga atttactgat gtatatttgg aactggtagt aagggaactg atgaacaagc
     1021 caattggcgc gatactggag tccgaagtca ggcagattaa ggagcttgag gcaagggtgt
     1081 gcgcaattcg cagcatagag gatctaaagt atttcacatc ccttgagaga cttgatttgt
     1141 atggaaacag aataagtgac ataagtgtac ttaagcatct gacaaatctt aagtatctgg
     1201 atcttagtca taattttagt tcgatttatg taggagacag ggaagggatg gtactggaac
     1261 ctcttagaaa ccttacacgt ttggaagtac tatatcttga taaaaacttt atctcggata
     1321 ttagtccgtt atccagcctt gtttcgctga aacgactgag cttgaaacag aacagaatat
     1381 ctgatgtgtc tcctttgaaa aggtgcgtga atctggaata tctggatttg tcaaaaaact
     1441 atcatgaagc cagtgaacaa gagtattatg gagttgaaga tctcacagag cttgcaaacc
     1501 ttcaaaagct tgtttatata gatattagtt ttaatactgt agaggacatt acaccgctat
     1561 cggtattgtc taatcttgag gtgttgttag ctggtgataa ctggattgaa gatatagagt
     1621 gcctaagagg acatgaaaaa ctaagagagc ttgatgtaca ccttaacatg atatcttact
     1681 ttgatgccat attggagatg ccttcgctca agaagctgtt ttatgataga aatccaatca
     1741 tggactatta tccaattgat ttgttcgaag gagtaattac accaactccg gatctgtcaa
     1801 ctccgcagcc cacatattga ttatattacc caattatgct agtcttactc caggcgttag
     1861 ccttgagcat ataaaataca tattggaaag gagcactacg caagtaggac attatgttga
     1921 ttgagaagat tagaaagaac agtgatgaat atggcagcat tcctttttgg agctggaacg
     1981 acaaacttga tcctgaagaa ttaagaagac aaataaatgt aatgaagaaa ataggcatga
     2041 acggcttttt catgcatgcc agaggtggtc ttgaaaccga ctatctgagt gatgactggt
     2101 atgactgcat aaggcaatgt gttgatgaag caaagaaact gggtatggaa gcctggtcct
     2161 atgacgaaaa cggctggccc agcggatttg ctggaggtaa actgctggaa gacaaaaaga
     2221 atcatgcaac gtatctgg
//
LOCUS       DAONVY010000402         2232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32738_length_2232_cov_2.546164, whole genome shotgun sequence.
ACCESSION   DAONVY010000402 DAONVY010000000
VERSION     DAONVY010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32738_length_2232_cov_2.546164"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PLI11_09505"
     CDS             complement(<1..1024)
                     /locus_tag="PLI11_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53144.1"
                     /translation="MNAYTLYCNVPCYLGLISYAEMARAAGNVEDAEKWEECAETIRK
                     AISERLAKGNRWSQDIFGFFHDPVITMMSDIFGYDLNDMPEDWVENSRNAYELDVRRI
                     RNQGHFGPSGIGYDHSMITQNALLLDMMDDANKLVSNLTKLSYAPGLPEPYLVPEGIT
                     VDVEKGIIRRQGDLGNLVQLAEALKCYHIVSGVSPVNNGKLKIMPRLPDEWNVEITDF
                     DLQNADGTLGMYITSPKNGTQTAQIKLQKVDLESVALRLGPFPADTKNVMARLNGQDV
                     ECRIVESGDRKWAWVEVPSDSIEDVEVAAIYSSTDDMPEWPEMPSWELPKKNESSPSG
                     ASINQIL"
     gene            complement(963..>2232)
                     /locus_tag="PLI11_09510"
     CDS             complement(963..>2232)
                     /locus_tag="PLI11_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53145.1"
                     /translation="PWGTANYPDMRFIGDEMGTLHIEYTNGDAEEIPLVFGYTMWFRH
                     HFSEGGAPFKTDEAVDGMKQLLDKTLHLYGGFEAAEKCVVKIKIQDKPIKSIKIVDNR
                     GKAGNPVFDGAYIVRGDFKGTLSGGAFSFEAGDAFFDKHVVDSKNPYPNEIKEALDEI
                     NKFLLTYEYEYENPPVYKYPSSYKGVKFRFTGNNLADIATGVVAENLFSLIDRVDDDG
                     MMHTSYKDAPSWRYDGFGTWVPNANSYYSDFYSRDGGRAIMTLNSYGYPGLGEKSVIF
                     ANKEMMYFREKGLTLNGVQIPGHYTVIVNKPLVYSTFLTEVAGWFTRYTKQAFGDEYQ
                     NMGNQETDGHGLMMIANYNIWRNLGMDKKWVEDNWRELKEAADFILWCFEHPRLSFAA
                     NGLLYAESRWYERIHPLLQRSLLPGSHQLC"
BASE COUNT          559 a          559 c          405 g          709 t
ORIGIN      
        1 ttaatatttg gttgatgctt gcgccagacg ggcttgattc attcttcttt ggaagctccc
       61 aacttggcat ttcaggccat tcaggcatgt cgtctgttga agagtaaatt gcagcaactt
      121 caacatcctc gatggaatct gatggcacct ctacccacgc ccatttcctg tctccagatt
      181 ccacaatcct gcattcaaca tcctgcccat taagtcttgc cattacattc tttgtatctg
      241 cgggaaatgg cccaagtctg agagctacac tttccagatc cactttctgc agcttaatct
      301 gagcagtttg tgttccgttc tttggtgatg ttatgtacat acccagtgtg ccatctgcat
      361 tctgaagatc gaagtccgta atttccacat tccattcatc tggaagtctt ggcattatct
      421 tcagtttgcc gttgtttaca ggtgacactc cagaaacaat gtggtaacac ttcaatgcct
      481 ctgcaagctg tacaaggttt ccgaggtctc cctgacgtct gatgatgcct ttttccacat
      541 caacagtgat tccttcaggt accaggtaag gctcaggcaa tccaggcgca tagctcagtt
      601 tggtcagatt gcttaccagt ttgtttgcat catccatcat gtcaagaagc aatgcgttct
      661 gtgttatcat tgagtgatcg tatcctattc cagaaggtcc gaagtgaccc tggtttctaa
      721 tcctcctaac atccaactca taggcgtttc tcgaattctc cacccagtct tcaggcatgt
      781 cattcaagtc gtagccaaat atgtcagaca tcattgtaat cactgggtca tggaaaaatc
      841 caaatatatc ctgactccat ctgtttcctt ttgccagtct ttcacttata gcttttcgaa
      901 tagtctctgc gcattcttcc catttttctg cgtcctctac attacctgct gctcttgcca
      961 tttcagcata gctgatgaga cccaggtagc aaggaacgtt gcagtaaagg gtgtatgcgt
     1021 tcataccacc tagactctgc atagagaagt ccgtttgctg caaaggaaag ccttggatgt
     1081 tcaaagcacc aaagaataaa gtctgccgct tccttgagtt ctctccagtt atcctccacc
     1141 catttcttat ccatgccaag atttctccat atgttatagt tggcaatcat cataagtccg
     1201 tgaccgtcag tctcctgatt tcccatgttc tgatactcat cgccaaatgc ctgttttgta
     1261 tatctggtaa accagcctgc cacttcggtc aggaatgtcg agtatacaag cggcttgttc
     1321 acaataaccg tataatggcc tggaatctgc actccattta aggtcaatcc tttttctctg
     1381 aagtacatca tttctttatt ggcaaatatg acactttttt ctccaaggcc tggataccca
     1441 tatgagttta aagtcattat tgcccttccg ccatctcgtg agtaaaaatc actgtagtat
     1501 gaatttgcat ttggtaccca ggttccaaaa ccgtcatacc tccaggatgg tgcatccttg
     1561 tatgaagtgt gcatcattcc atcatcatcc acacgatcta tgaggctgaa caagttttct
     1621 gcaaccactc ccgtagctat atcagctaga ttgttgcctg taaatctgaa tttaacacct
     1681 ttatatgatg aaggatactt gtatacagga gggttctcgt attcatactc ataagttaaa
     1741 agaaatttgt ttatctcatc caatgcttct ttgatttcat tcggataagg attcttggaa
     1801 tcaaccacat gtttgtcaaa gaatgcatct cctgcctcaa aagagaatgc tcctccacta
     1861 agggtgcctt tgaaatcccc tcttacaata taagcaccgt caaatacagg atttcccgcc
     1921 ttccctctgt tgtctacaat tttaattgac ttaattggct tgtcttggat tttaatcttt
     1981 acaacacact tttccgcagc ttcaaatcca ccataaagat gaagggtttt atccaaaagc
     2041 tgcttcatcc cgtctacagc ctcgtcggtt ttgaaaggag ctccaccttc tgaaaagtga
     2101 tgcctgaacc acatggtata gccaaatacc aagggtatct cttccgcatc gccatttgta
     2161 tattcaatat ggagggttcc catttcatcc cctatgaacc tcatatcagg ataattggca
     2221 gttccccatg gc
//
LOCUS       DAONVY010000403         2230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32768_length_2230_cov_3.605977, whole genome shotgun sequence.
ACCESSION   DAONVY010000403 DAONVY010000000
VERSION     DAONVY010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32768_length_2230_cov_3.605977"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..746
                     /locus_tag="PLI11_09515"
     CDS             117..746
                     /locus_tag="PLI11_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53146.1"
                     /translation="MSKKLIITMVLVMSIIGSIMAFAAPTDIPLNGTGAGVGAPQITA
                     TDHLGVKFAVPEGKVLLKFSIVNMPTYESNNAYYTFKLFAWNTDFDTTVSGTPLYTKK
                     VENQHDNVTATFEFPEALPAGEYLAHVSEGGVTEGTGCPAVWTSGPKEGVTTFWSGKQ
                     RDDFTANVAITVDDAPNPQTSDSILILPFVLAVMLPVAVLTNTRKSVSA"
     gene            complement(784..2043)
                     /locus_tag="PLI11_09520"
     CDS             complement(784..2043)
                     /locus_tag="PLI11_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53147.1"
                     /translation="MKKYFIAFFALSLGLIAVGCSNQPGKPTPEPTATPKSDYILALE
                     ERYKDYQEVSYGLDITLKEDLVSICYSTWFNPVVSKTNPVIYNITEILEQEKKGETPQ
                     WGPEHAFHYWAKPALGYYRSDDKDVIRAHMKQLYEAGVDFIIIDNTNAQATTWQAGVE
                     NSYWDRMVTQSCTALLDTIVEMREEGLQTPYVVFWNRVDSNIGWATPKRVYEEFYTND
                     KYKDCWVYWDGKPFMAVTRLTNEPGIASVDRFKYKDNLTLRQMTVAIFRKGQQLKQHE
                     WSFLYDGSVPCADDLGFYEHISVYVARQENYMSNTKSAIGRNGGVTFYNQWKRAFEYH
                     PKVVTITWWNEWVAQRFLVDIDGDGKPESHFVDNYNIEYSRDIEPMEGGHGDQYYRWM
                     KEYIEAYKARKKCPVLVEKGYEDKIKK"
BASE COUNT          618 a          453 c          437 g          722 t
ORIGIN      
        1 aataaatatt tacgaaataa tgaataaagg gtgttgtaaa agcgtgtaaa ttaatttata
       61 ataaatttga acaaaaaatt aacgaacaaa aaattgtgaa taaggaggca attcttatgt
      121 caaaaaaatt aataataacc atggttttgg ttatgagtat tattggatca ataatggctt
      181 ttgcagcacc tactgatatt ccactgaatg gaacaggtgc cggggttggt gctccacaaa
      241 ttacagctac tgaccatctt ggtgtcaagt ttgcagttcc tgaaggtaaa gtattgctta
      301 agttctcaat agtaaatatg cctacatatg aatctaacaa tgcatactat acattcaagc
      361 tgtttgcatg gaatactgat tttgatacta cagtaagtgg aacgccattg tatacaaaga
      421 aggttgagaa tcaacacgat aatgtaactg ccacgtttga attcccagag gctcttccgg
      481 ctggtgaata cctggcacat gtcagtgagg gtggagtaac cgagggtact ggatgtcctg
      541 cagtctggac ttcaggtcca aaggaaggcg taaccacttt ctggtcaggt aagcaaagag
      601 atgactttac cgccaatgtt gctatcactg tagatgatgc tccaaatccc caaacaagtg
      661 acagtatatt aattcttcct tttgtattgg ctgtaatgct gcctgttgca gtattaacaa
      721 atacaaggaa aagcgtgtca gcataactta aagccggcga gagccggctt attattacac
      781 tctctatttt tttatcttgt cttcataacc tttctcaaca aggacgggac atttttttct
      841 tgccttgtat gcctctatgt attctttcat ccagcggtaa tactgatcgc catgaccgcc
      901 ttccattggt tcgatatctc tactgtattc aatattgtag ttgtcaacaa agtgagattc
      961 cggttttcca tcaccatcta tatcaaccag aaacctttga gcaacccatt cattccacca
     1021 ggttatagta actacctttg gatgatactc aaaagccctt ttccactgat tatagaaggt
     1081 tactcctcca tttcttccta tggcggattt ggtattggac atgtagtttt cctgccttgc
     1141 aacatatacg ctaatgtgtt catagaaacc caagtcgtcc gcacatggca cggaaccgtc
     1201 gtacaggaat gaccactcgt gttgtttgag ctgctggcct tttctgaaaa ttgcaaccgt
     1261 catctgtcga agagttaaat tatctttata cttgaatcta tccacgctgg cgattccagg
     1321 ctcgtttgtc agtcttgtta cagccataaa gggctttcca tcccagtata cccagcagtc
     1381 cttgtacttg tcgttggtgt agaattcttc ataaactctt ttgggagtcg cccacccaat
     1441 atttgagtcc actctgttcc agaagacaac gtatggtgtt tgcaacccct cttccctcat
     1501 ttcaactatt gtatccaaga gagcggtgca ggattgggtg accatcctgt cccaatagct
     1561 gttttcaacg cctgcctgcc atgttgttgc ctgtgcattt gtgttatcga tgattatgaa
     1621 atcaacacct gcttcataaa gctgtttcat gtgagctctt attacatcct tgtcatcgct
     1681 tctgtaatac cctagagcag gctttgccca atagtggaag gcgtgttcag gaccccattg
     1741 tggtgtttca ccctttttct cctgttccaa tatttctgtg atgttgtaga ttaccgggtt
     1801 tgttttgctt acaacaggat tgaaccaggt tgagtaacag attgatacaa ggtcttcctt
     1861 taatgttatg tcaaggccgt aggatacttc ctgataatcc ttgtatctct cttccaaagc
     1921 taatatgtaa tctgattttg gtgtagctgt tggttcggga gtgggttttc ccggctgatt
     1981 actgcatcca acagctatca atccaaggga taaagcaaag aaagcaataa aatatttctt
     2041 catggttatc ctccgtgctt gtttttctta catatataat atgattattg ctgacaaaat
     2101 acaaggacaa aagcaaattt aaaactttat aattctcctt ttctgtgata tattagtttt
     2161 aaacgaatga agggagtcaa ttatgaacaa aagggtcttg gctaatgtta ttgcagtagt
     2221 gttgataatc
//
LOCUS       DAONVY010000404         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32807_length_2228_cov_2.104464, whole genome shotgun sequence.
ACCESSION   DAONVY010000404 DAONVY010000000
VERSION     DAONVY010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32807_length_2228_cov_2.104464"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1813
                     /gene="ftsH"
                     /locus_tag="PLI11_09525"
     CDS             17..1813
                     /gene="ftsH"
                     /locus_tag="PLI11_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPZ53148.1"
                     /translation="MKAFKNISVYIIIGALVIFLVIMFENIISVNTLSYTEFIKQLDA
                     DNVAEVKLEKNTATVKLKEPVKGAKEYKVTIYSQEVFEELMIEASRKSEDFKYSTTVR
                     EIPFWVTLLPYILMVGLFVVFLFIFMNQGNSGANRVMNFGKSRARIYTTSNITFADVA
                     GAEEEKEELMELVSFLRDPKRYSSLGARIPKGVLMVGPPGTGKTYLAKAVAGEAGVPF
                     FSISGSDFVEMYVGVGASRVRDLFEQAKKNTPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFGINEGIIIMAATNRPDILDPALTRPGRFDRKITVGYPDIKAREL
                     MLKVHAKNKSLSSDVDLSEVAKNTSGFTAADLENLMNEAALLAARNRRDEITNEDIKE
                     ATFRVIVGPEKRSRVMTDKDKKLTALHEAGHAIAVKLISSTEKVDRISIIPAGLAGGY
                     TASRPDEDRAYTTKSQLLEEIVIALSGKAAEELVLNEVSTGASSDLKRANKIAHNMVA
                     KYGMSENLGNVIVDTGSNEVFIGKDYAHTRNYSEETAALIDKEVKKVIDSCYEKAKQI
                     LTEDIDKLNKVADILLVKEKIEGDEFDRIMAE"
     gene            1985..>2228
                     /locus_tag="PLI11_09530"
     CDS             1985..>2228
                     /locus_tag="PLI11_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPZ53149.1"
                     /translation="MNVETIQGTIVSIVFENDNGFKICDLDTGDCLITIKGSLPSVQV
                     GETISAVGRFEVHKRYGEQFVVESYTKELPTGYDEII"
BASE COUNT          716 a          376 c          563 g          573 t
ORIGIN      
        1 acattggagg tagattgtga aggcgtttaa aaatatatcg gtatatatta taatcggagc
       61 gctggtcata tttctggtca ttatgttcga aaatattata tcggtaaata cccttagcta
      121 cacggaattc ataaaacagc tggatgcgga caatgtggct gaagtcaagc tggagaaaaa
      181 cactgcaacc gtaaagttga aagagccggt aaaaggagca aaagagtata aggtaacaat
      241 atattcccag gaagtatttg aagagttgat gatagaggca agcagaaagt ctgaggactt
      301 caaatattca accactgtcc gggagattcc tttctgggtg actttgttgc catacatcct
      361 tatggttggg cttttcgtgg tcttcttgtt tatctttatg aatcaaggaa attccggcgc
      421 aaacagagtc atgaactttg gaaaaagccg tgccagaata tatacaacaa gcaacataac
      481 ctttgcggat gttgctggtg ctgaggaaga gaaagaagag cttatggaac ttgtatcgtt
      541 cctgagggat cccaaaagat attcctcttt gggtgccaga attccaaaag gtgttttgat
      601 ggtaggacct ccaggtacag gaaagactta tcttgcgaaa gctgttgcag gagaagccgg
      661 tgttccattt ttctcaatca gtggttctga ttttgtagaa atgtacgtgg gtgtgggagc
      721 atccagagta agagatttgt ttgaacaggc aaagaagaac actccctgca taatctttat
      781 cgatgagatt gatgcggtgg gacgtcatag aggagccgga cttggtggcg gtcatgacga
      841 aagagaacag acattgaacc agctgcttgt tgaaatggac ggatttggta ttaatgaagg
      901 tataatcatc atggctgcaa caaacagacc ggacatactg gatcctgcac taacgcgtcc
      961 aggacgcttt gacaggaaga taaccgttgg ctatcctgat ataaaggctc gtgaacttat
     1021 gctcaaggtg catgcaaaga acaagtccct tagcagcgat gtggaccttt ccgaagtagc
     1081 aaagaataca tcaggattta cagcggctga cttggaaaac cttatgaatg aggcggctct
     1141 tttggctgca agaaacagaa gagatgagat aaccaatgag gacatcaagg aagctacttt
     1201 cagagttatc gtaggtccgg agaagaggag cagggtaatg accgacaagg acaagaaact
     1261 gactgcgctt catgaggctg gacatgcaat agcggtaaaa cttatttcaa gtactgaaaa
     1321 ggtagacaga atatcgataa tccctgcagg actggcagga ggatatacag cctccagacc
     1381 tgatgaggac agggcgtata ctacgaagtc tcaacttctg gaggaaattg ttattgcgct
     1441 gagtggaaag gctgctgaag aacttgtgtt gaacgaggta agcaccggag catcttcaga
     1501 ccttaaaagg gcaaacaaga ttgctcacaa catggtggcc aagtatggta tgagtgaaaa
     1561 ccttggcaat gtgattgtgg acactggcag caacgaggta ttcataggaa aagattatgc
     1621 ccatacaaga aattattcag aagaaactgc tgcactaata gacaaggaag tcaagaaagt
     1681 aatcgactcc tgctatgaga aagcaaagca aatattgaca gaggatattg ataaacttaa
     1741 caaggttgcc gacatcctgc tcgtgaaaga gaaaatcgaa ggggatgaat ttgacaggat
     1801 catggctgaa taagagatgc agcattacac atacctaaac ctgatatcat ataatgatat
     1861 tggaggtgta gtgtcatgct gttttttctt tactcggtaa tcatcatgat gactgtaatg
     1921 cacttgatga tttttttcag tatttggttt tatcgtcagg aagaatgtga ttcggggtag
     1981 taagatgaat gtcgaaacca ttcaaggaac aattgtatcc atagtgtttg aaaatgataa
     2041 tggctttaaa atatgtgacc ttgatacagg agattgtctc attacaataa aaggaagttt
     2101 gccttccgtg caagttggtg agacaatttc tgctgtgggc agatttgagg ttcacaaacg
     2161 ctatggtgag caatttgttg tggagagcta caccaaggaa ctgccaacag gatatgatga
     2221 aataataa
//
LOCUS       DAONVY010000405         2226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32835_length_2226_cov_3.653616, whole genome shotgun sequence.
ACCESSION   DAONVY010000405 DAONVY010000000
VERSION     DAONVY010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32835_length_2226_cov_3.653616"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLI11_09535"
     CDS             complement(<1..231)
                     /locus_tag="PLI11_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPZ53150.1"
                     /translation="MKIGMVSLGCAKNQVDAEIMLGQIKNRNHEIVVDPDEAEVIVVN
                     TCAFIESAREEAINAILEMASKKTTGSLKYLIV"
     gene            complement(318..923)
                     /locus_tag="PLI11_09540"
     CDS             complement(318..923)
                     /locus_tag="PLI11_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPZ53151.1"
                     /translation="MENENGLYVIECNKNDDGKYFVTFSNGETETFSEDEVMEHCLYD
                     ISVPLNCSFEDLVIKIKKRRALKSIVGYVARTLRTEREVRERLLFLGYNDAVIEETVA
                     YLKTQGYIDDREYARKYVNTSLKTKIESRKMCMYKLVQKGISDDIAEEAVRVIDDNEQ
                     IQKLMQKKSVRQKEKSKLRGYLYSKGFSVESINRVLGDDTY"
     gene            complement(932..2011)
                     /gene="recA"
                     /locus_tag="PLI11_09545"
     CDS             complement(932..2011)
                     /gene="recA"
                     /locus_tag="PLI11_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPZ53152.1"
                     /translation="MDEKKVDEKKVAERRKALENAIAQIDRQYGKGAVMRLGENVHMN
                     IEVIPTGALSLDLALGVGGLPRGRIIEIYGPEASGKTTVALHVIAEAQRLGGDAAFID
                     AEHALDPVYAKALGVDIDNLIVSQPDTGEQALEITEALVRSGAIDVIVIDSVAALVPK
                     AEIDGEMGDAHVGLQARLMSQALRKLSGVINKSKCIAIFINQLREKVGVMFGNPETTS
                     GGRALKFYSSIRLDVRRVEALKSGTEIIGNRTKVKVVKNKVAPPFKETEFDIIYGEGI
                     SKEGCILDVAVEKDIIKKSGSWFSYGDQRIGQGRENAKVFLRENPEVTLEIETAIRKL
                     VEEEKEKKRALMEPDNPFDPKSTKG"
BASE COUNT          533 a          564 c          342 g          787 t
ORIGIN      
        1 aactatgagg tatttaaggc ttccagtagt cttttttgat gccatttcaa gtattgcatt
       61 tattgcttct tccctggcac tttcaataaa agcacaggtg tttaccacaa ttacttccgc
      121 ttcatctgga tcaaccacta tttcatgatt cctattttta atctgaccca gcattatttc
      181 agcatccacc tgattttttg cacatcctag agataccatt cctattttca tctatataca
      241 aactcctaac taatctgcaa actaaacagc gtctttaacc caattatttt ataccattct
      301 gagcaaattg tccatattta atatgtgtca tcacccagca cacggtttat gctttcaaca
      361 ctaaaccctt ttgaatagag atatcctcta agttttgact tttccttttg ccttaccgat
      421 ttcttctgca taagtttttg tatctgctcg ttatcgtcta tcaccctgac tgcttcttca
      481 gcaatatcat ccgatatgcc tttctggaca agtttgtaca tgcacatttt tcttgattca
      541 atttttgtct ttagtgaagt gttaacatac tttctggcat attccctatc gtcaatatac
      601 ccttgggttt tcagataggc cacggtttcc tcaataacag cgtcgttata tcctaaaaac
      661 aaaagcctct cccttacttc cctttctgtt ctcagggtgc gtgcaacata gccaacaatt
      721 gactttaacg ctcttctttt ctttattttt atcacaagat cctcaaaaga acagtttaat
      781 ggaacactga tatcatatag acagtgttcc attacctcat cctcgctaaa agtttcggtt
      841 tctccgttgc tgaaagttac aaaatatttt ccatcgtcat ttttattgca ttcgatgaca
      901 taaagtccgt tttcattttc cacggcttgt ctcacccttt ggtagatttc ggatcaaacg
      961 gattgtcagg ttccatcaga gcgcgcttct tttccttttc ctcttcaacc agtttgcgta
     1021 tggctgtttc aatttccaaa gttacttccg gattctctct caagaaaacc tttgcattct
     1081 ctctaccctg accgatgcgc tgatctccgt atgagaacca cgagccgctc ttcttgataa
     1141 tgtctttttc aacagccacg tccagtatgc agccttcttt tgatatgcct tctccataaa
     1201 ttatgtcaaa ttccgtctcc ttgaaaggtg gcgcaacctt gttctttacc accttcacct
     1261 tggtcctgtt tccaattatt tcagtaccac tcttgagtgc ctccacgcgt cttacatcaa
     1321 gccttatgga tgagtagaac ttcaaagctc gtcctcctga tgttgtttca gggttgccaa
     1381 acataactcc taccttttca cgaagctggt taatgaatat tgcaatacat tttgatttgt
     1441 ttattacgcc ggaaagcttt ctgagagctt gggacataag tcttgcctga agtccgacat
     1501 gtgcgtctcc catttcaccg tctatctcgg cttttggaac cagagcagcc accgagtcta
     1561 ttacaattac gtcaatagcg ccacttctta caagggcttc agtaatctcg agagcttgtt
     1621 cacctgtatc aggctgggat actataaggt tgtctatgtc caccccaaga gcttttgcat
     1681 atacaggatc caatgcatgc tcagcatcaa taaatgcggc gtctccaccc agcctctgag
     1741 cttctgcaat aacatgtagt gctacagttg tcttgcccga tgcttccggt ccatatatct
     1801 cgattatcct gccccttggc aatcctccca cacccagcgc cagatccaga ctcaaagcac
     1861 cagtggggat tacctcaata ttcatgtgaa cattctcgcc tagtctcatt acagcaccct
     1921 tgccgtattg tctgtctatc tgcgcaattg cattttccaa agcttttctt ctttcggcta
     1981 ccttcttctc atctaccttt ttttcatcca tgtcttttat cccctcatag tcttttattc
     2041 gaacataggt tcctctatat attatactta ttaaagggag gtgtcaatat aggattaaag
     2101 cttcgggaaa tatccgccaa tatttcccga agctttttct tttacaaaaa cttggtggta
     2161 ttccttattt ttctattctc aatccctcaa gtatgttctt tttcttcaac ttgttcatgg
     2221 agtatg
//
LOCUS       DAONVY010000406         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32913_length_2222_cov_2.915551, whole genome shotgun sequence.
ACCESSION   DAONVY010000406 DAONVY010000000
VERSION     DAONVY010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32913_length_2222_cov_2.915551"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLI11_09550"
     CDS             complement(<1..505)
                     /locus_tag="PLI11_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPZ53153.1"
                     /translation="MRYLLIRPRYKNILANLEPLGLEYVGGVLNDLEKEHLIYDEFSH
                     INLFKKTRLRRIIKSFKPEVVGFTAHVNTVNIINKMACIIKKLNPDIRIIVGGPHAEL
                     NYDEFFTPCIDYIFTDSDLSSFYVAAENNFNHDAVTNLTGVCYREKGEWFVNPKSKPA
                     RDFNARPD"
     gene            complement(502..1056)
                     /locus_tag="PLI11_09555"
     CDS             complement(502..1056)
                     /locus_tag="PLI11_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPZ53154.1"
                     /translation="MDRIFGYISYMMPFMLIALVIYIIIRLIVVKKRKKRISIKREIP
                     LMLFISYITGLLSITAFPKVVFNDGIRLVVGPGSVNLIPFRTIVTYFDERFIIFAILF
                     AGNIVMFMPVGFFVPLIWKKGPLFTTMCGLLFSLFIEISQQFVVRNTDIDDLILNTFG
                     VYLGYLLYAAAKHIINRSKKSEEL"
     gene            1380..1454
                     /locus_tag="PLI11_09560"
     tRNA            1380..1454
                     /locus_tag="PLI11_09560"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1412..1414,aa:Gln,seq:ttg)
     gene            1620..>2222
                     /locus_tag="PLI11_09565"
     CDS             1620..>2222
                     /locus_tag="PLI11_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53155.1"
                     /translation="MNGKKLVIFLFSLLLLISLVSCKSAPADTPEDTPTPEPTATPVP
                     TDTPEPTPSPTPKPTREPHSDENPYYGSIVSEDPVRVPLFTHNDPNAVHLPEQIFEHT
                     TVAWQFVATTHFNAIELCMCTWHEPDGATNFSIYKWNYDYDKTISGDPLVTYVKENIQ
                     DNQEVKFELEEPLPPDEYLVVINDNLGTKIGLWKVPYNEDE"
BASE COUNT          713 a          427 c          352 g          730 t
ORIGIN      
        1 tgtcaggtct tgcgttgaaa tcccttgcag gttttgattt tggatttaca aaccattctc
       61 ctttttccct gtaacacaca cctgtcaagt tcgtaactgc atcatgatta aaattgtttt
      121 cagcagcgac ataaaaactt gaaaggtcac tatctgtaaa tatgtagtct atacaaggtg
      181 tgaaaaactc atcataatta agttctgcat gagggcctcc aacaatgatt ctaatgtctg
      241 gattcaactt ctttattata catgccattt tgttaattat gttcactgtg ttaacatgtg
      301 cagtaaatcc aacaacctcc ggcttgaagc tttttataat tctccttaat cttgttttct
      361 taaacagatt gatatgtgaa aactcatcat aaatcaagtg ttctttttcg agatcgttaa
      421 gtacacctcc cacatactcc agtcctagag gctccagatt tgccagtatg tttttatatc
      481 tcggtcttat taataaatat ctcataattc ctcacttttc ttgcttcggt taattatatg
      541 ctttgcagct gcataaagta aatagcctaa gtatactcca aacgtgttta aaatcagatc
      601 atcaatatcc gtatttctga ctacaaactg ttgagaaatt tcaataaaca gcgaaaataa
      661 aagaccgcac attgtggtaa acaaagggcc tttcttccat atgagcggaa caaagaagcc
      721 aacaggcatg aacatgacaa tgttgcctgc aaacaatatc gcaaagatta tgaatctctc
      781 gtcaaaatac gttacaattg tcctaaacgg aatcaaattg acacttccag gacctacgac
      841 caatcgtatt ccatcattaa atactacttt tggaaaagct gttatggaca ataatcctgt
      901 gatataggat ataaagagca ttaatggtat ttccctctta atacttatcc ttttcttgcg
      961 ctttttaaca actattaacc ttataattat gtaaataaca agagctataa gcataaaggg
     1021 catcatgtaa cttatgtatc caaaaattct atccataata atctccttta atctccagat
     1081 atgaacaaga atggcacgaa tattctcgag catgcagttg caaatacact cttgaactct
     1141 aattctttaa aaaatatagt aaacaggatt ctaatatatc tgtattacta ttgaagtgtc
     1201 aagtaccaca actatatggc gaagatataa ctgtaagtat gacaagtgta gtttaagtta
     1261 tattatttca tgttggacgt aaactgcata aatgtaaata tctgttattt atataaaaat
     1321 tacattttac cgttgacaaa ttcctggata atgtgtataa tatcttgcgt tggtgttatt
     1381 gggatgtagc caagcggtaa ggcaccagac tttgactctg gcagtcgcaa gttcgaatct
     1441 tgccatccca gccagtagac tgtcaaatga cagtcttttt ttatgtcttt tcatctttta
     1501 tgttcatttt ttattcttat tgtttctaat tttgttcgtt ttgttgatat ttattgttga
     1561 taatttaata taattgtatt atattatatg tatcttcaca tcgaaaagga ggatttttta
     1621 tgaatggcaa aaagttagtg atttttctgt tttcgttatt actactaatt tctctggtgt
     1681 cttgcaaatc tgctccagca gatactcctg aagacacgcc aacaccagaa ccaactgcga
     1741 cacctgtccc tactgatacc ccagaaccca ctccatcacc tacacccaaa ccaacaagag
     1801 agccacattc agacgaaaat ccatattacg gttctattgt atcagaggac ccagtaagag
     1861 tacctttatt cacacataac gatcctaatg cggttcattt accagaacaa atatttgagc
     1921 ataccaccgt tgcatggcaa tttgtagcta ccacacactt caatgcaatt gaattgtgca
     1981 tgtgtacatg gcatgagcct gatggtgcta ccaacttttc aatatacaaa tggaattatg
     2041 actatgacaa aactatttca ggcgacccct tggtgactta tgtgaaagaa aatatacagg
     2101 acaatcaaga ggtaaagttt gaacttgaag agccactacc tcctgatgag tatcttgtcg
     2161 taatcaatga caatcttgga acaaaaatag gattgtggaa ggttccgtat aatgaagatg
     2221 ag
//
LOCUS       DAONVY010000407         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_32932_length_2221_cov_2.962604, whole genome shotgun sequence.
ACCESSION   DAONVY010000407 DAONVY010000000
VERSION     DAONVY010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32932_length_2221_cov_2.962604"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /gene="pgk"
                     /locus_tag="PLI11_09570"
     CDS             complement(<1..366)
                     /gene="pgk"
                     /locus_tag="PLI11_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPZ53156.1"
                     /translation="MSYYNKKNVTDINVTGKRVIVRVDFNVPFDTEGNISDDKRIVAA
                     LPTIKYLLDKKAKVILMSHLGRPKGQFNMKYSMASCSKHLGKLLNMEVIQSSDVIGPD
                     SKEKAKNLKEGQILMLENVR"
     gene            complement(457..846)
                     /locus_tag="PLI11_09575"
     CDS             complement(457..846)
                     /locus_tag="PLI11_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53157.1"
                     /translation="MIVLFLCVLTVCAYFDIKTRKIPIAALLLLIVVSLLRVLVIGMP
                     DLLSFMMCGTVVFVCTILKKAVGKADVVCIMCAFLMFDASTAAKGLFMACMLAFLSGL
                     AYYKGKARGEELPFVPFLLLGMLFSLH"
     gene            complement(843..>2221)
                     /locus_tag="PLI11_09580"
     CDS             complement(843..>2221)
                     /locus_tag="PLI11_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPZ53158.1"
                     /translation="RPSVIMQRIKNIFPSLEVVKDYNEEAMERLTLPLPFLTENAGLL
                     RNAEDETIKCIRKWYRERDEFKDVFERILKADTSFRKDLYLPKDIMDELFARNLYMSA
                     SKIEKYNKCPYSYFMRYGIKAEKRKEALVENTDVGTFMHTLIDKASKKAVDLPVENSD
                     KLMDDVFFESLEEVGIRSFYDTERKKFIANRLLRYAKKAFRKALWQIHGGVFQPAGFE
                     VAFGPDGELPPVRVRLDDGRYVQITGRIDRYDVCETEDAIYLRVIDYKSSDLNVSPQD
                     LINGQSIQLVAYMDALVEGIGSKSEKAVVPVACLYFTLGPDVKSASNRSEVSREEELR
                     LKGYIIDDPEVLDCIVSDEEDAKTMSIRRKKEGWSVTLKGGAVPRNGFDLLRKQLHKT
                     VKNTYERMAEGDISIIPVDFPNKNFKVCDYCEYITVCGIDTSKPENFRKQPQYDVDEA
                     YTQMGSDD"
BASE COUNT          627 a          554 c          358 g          682 t
ORIGIN      
        1 cctgacgttt tcaagcataa gtatttgtcc ttctttcaaa ttctttgcct tttccttgct
       61 gtcaggacct attacatcgg aagactgtat aacctccata ttcaacagct ttccaagatg
      121 cttggagcat gaagccattg aatacttcat gttgaactgt ccctttggcc tgcccaggtg
      181 ggacataagt attaccttgg ctttcttgtc caacaaatat tttattgtag gcaaagccgc
      241 tactattctt ttgtcgtctg atatgtttcc ttctgtatca aagggtacgt tgaagtcaac
      301 ccttactatg actcttttgc cggttacatt tatatctgta acgtttttct tgttgtaata
      361 gctcatatat cagccactct ccttaaattc tgtatatcta aaatctagtc aaaagaccca
      421 aattctattt tatacctaat atccaaaaat atcaacctaa tgtaaagaaa ataacatgcc
      481 aagaagaaga aaaggaacaa aaggcagttc ttcccccctt gctttcccct tgtagtatgc
      541 caatccagac aaaaacgcca gcatgcaggc cataaacaaa cctttggcag ctgttgacgc
      601 atcaaacata aggaaagcac acataatgca cacaacatcc gccttgccca ccgctttctt
      661 gagtatggta caaacaaata caactgtgcc acacatcata aacgacaata agtctggcat
      721 tcctataacc aacactctta aaagcgaaac tacaattaac aagagcagtg cggcaatagg
      781 tatttttctt gttttaatgt caaaatatgc acaaacagtc aaaacacata agaaaagtac
      841 tatcaatcgt cgctgcccat ctgcgtatag gcctcatcaa cgtcatattg gggctgtttt
      901 ctaaaattct caggcttgga tgtatcaatt ccgcatacgg ttatatactc acaataatca
      961 caaaccttga agttcttgtt cggaaaatca acaggaatta tggagatgtc tccttcggcc
     1021 attctttcgt aagtattttt aacagtcttg tgaagctgtt tccttaaaag gtcgaatccg
     1081 ttcctgggaa cggctccccc tttcagagta accgaccatc cttccttttt ccgtcttata
     1141 ctcatggtct ttgcatcctc ttcatcggaa acaatacaat caagaacttc cggatcatca
     1201 ataatatatc ccttcagtct cagttcctct tccctggaca cttcacttct gtttgaagca
     1261 ctctttacat ccggacctag tgtgaagtac aggcaggcaa caggaacaac cgctttttcg
     1321 cttttgctgc cgatgccttc caccaaggcg tccatgtaag caaccagttg tatgctctga
     1381 ccattaatca aatcctgggg ggatacatta aggtcagatg acttgtaatc tattaccctt
     1441 aaataaattg catcctccgt ctcacagaca tcgtaccggt caatccttcc tgttatctgg
     1501 acgtatcttc catcatccag cctgacacgt actggaggca actctccatc aggaccaaac
     1561 gccacttcaa atccggctgg ctggaaaacc cctccatgta tctgccataa ggcttttctg
     1621 aatgcctttt ttgcatatct taaaagcctg tttgcaataa actttttcct ctctgtgtcg
     1681 taaaaactcc ttatgcccac ttcctccaag gattcaaaaa acacatcatc catgagcttg
     1741 tcgctgtttt caaccggaag atcgacagcc ttttttgaag ccttgtcaat aagtgtatgc
     1801 ataaaagtgc ccacatccgt attttcaacc aacgcttcct tccttttctc cgcctttatg
     1861 ccatagcgca tgaagtagga ataggggcac ttgttgtact tttcaatttt ggaagctgac
     1921 atgtaaaggt tcctggcaaa aagctcgtcc attatgtctt ttggcagata caaatctttt
     1981 ctgaaagaag tgtccgcttt cagaattctt tcaaagacat ccttgaactc atccctttcc
     2041 ctataccatt ttcttataca ttttattgtc tcatcctctg cattccgcaa aagccctgca
     2101 ttctcagtca aaaaaggaag aggcaaagta agtctttcca ttgcttcctc attgtaatcc
     2161 tttacaacct ccaatgacgg aaagatattc ttaattcgct gcataataac ggacggtctc
     2221 a
//
LOCUS       DAONVY010000408         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_33501_length_2192_cov_3.150211, whole genome shotgun sequence.
ACCESSION   DAONVY010000408 DAONVY010000000
VERSION     DAONVY010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33501_length_2192_cov_3.150211"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..968)
                     /gene="rbsK"
                     /locus_tag="PLI11_09585"
     CDS             complement(24..968)
                     /gene="rbsK"
                     /locus_tag="PLI11_09585"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPZ53159.1"
                     /translation="MAKIVVTGSYITDIAAITSRFPRDGETVIGETVKIGPGGKGSNQ
                     ATACKRLGAEVVLITKTGNDYLRNVALNHYKNENMSDKYVFVDEENQTGVAIIQIHKS
                     SAENRILVTPGANNHITEEDVYKAEEEFKTCDIVLLQLEASMESVRASINLAKKYNKT
                     VVLNPAPVKELDDEMFGLIDYFTPNETEAEFYSGLKADSIQNIEKMAELFIAKGVGNV
                     IITLGKAGAFVKTRDAACIIPAYTVNAVDTTGAGDAFNGGFCVALAEGKGIVEAARFA
                     NAVAALSVTKLGASESMPDRLDVDSFLKNRLCKLNMKK"
     gene            complement(973..1620)
                     /gene="deoC"
                     /locus_tag="PLI11_09590"
     CDS             complement(973..1620)
                     /gene="deoC"
                     /locus_tag="PLI11_09590"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPZ53160.1"
                     /translation="MDIAKYIDHTNLKSTATVGDIERLCEEARKYGFYSVCVNPYYVS
                     LCKRLLSGTDVKVCTVIGFPLGATTTSTKVFETEETLKMGCDEFDMVINVGMLKSGKD
                     DHVMQDIRQVVEAAKDRTVKVIIETGLLTDEEKMRACRLASQAGAHFVKTCTGMTQGK
                     ALVEDIDIMKKNIVPGVKIKASGGIRTYEDAIKLIEAGSDRIGTSSGVEIVNNGR"
     gene            complement(1635..>2192)
                     /locus_tag="PLI11_09595"
     CDS             complement(1635..>2192)
                     /locus_tag="PLI11_09595"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HPZ53161.1"
                     /translation="GKGYTFYGPGVSQGHSDAVRRVDGVLDARQYTIPVEDVVNRVRN
                     GETPDFTKREMHERHCYVVVKEEADKEKIKKEIVNMPGYFADYDTFVEYVSKEELLEN
                     HAKLPHGGFVITSGKTSEDNQHIIEFSIKLESNPEFTSAILVAYARAAYKLSQQGSIG
                     CHTIIDVPISLISTLSREEILSMIL"
BASE COUNT          600 a          551 c          330 g          711 t
ORIGIN      
        1 aagcaattct ataaaacatt gtgctatttt ttcatgttta gtttacaaag cctatttttt
       61 aaaaaagaat caacatccag tctgtctggc attgattccg atgcaccaag ttttgtaacc
      121 gacaaggcag caactgcatt tgcaaatctt gcagcttcaa caataccctt gccttctgcc
      181 aaagcaacac aaaatcctcc attaaacgca tctcctgctc cggttgtgtc cactgcgtta
      241 accgtatatg caggaataat acaagcagca tcccttgttt taacaaacgc tcccgccttg
      301 cccaaagtga taattacatt cccaacacct ttggcaataa acaattctgc cattttctca
      361 atgttttgaa tggagtcagc ctttaatccc gaatagaatt cggcttcagt ttcattcgga
      421 gtaaagtagt caattaagcc aaacatttca tcatccagct ctttaacagg agcaggattc
      481 aaaacaactg tcttgttgta tttctttgca agatttattg aagccctgac tgattccatg
      541 cttgcttcca attgcagaag aacaatatcg caagtcttga actcttcttc cgctttatac
      601 acatcctctt cagtaatgtg gttgttggcc cctggcgtta ccaatattct gttttcagca
      661 ctacttttat gtatttgaat aattgcaaca cccgtctggt tttcctcatc aacaaacacg
      721 tacttgtcag acatgttttc attcttgtaa tggtttaaag ccacattacg caaatagtcg
      781 ttgcctgtct ttgtaatcag gacaacctcc gcacccaaac gcttgcatgc agttgcctgg
      841 tttgaacctt ttccaccggg ccctattttc acagtctccc ctataacagt ttccccatct
      901 ctaggaaaac gtgatgttat ggctgcaata tctgtgatat atgagcctgt aacaacaatt
      961 ttagccatgg tatcaccgtc cgttattcac tatttcaaca ccgcttgatg tacctatcct
     1021 gtcactgcca gcctcaatca attttatggc atcctcatac gtccttattc cacccgacgc
     1081 ctttatcttg acaccgggca caatattttt cttcataata tcaatatcct ctaccagagc
     1141 ttttccctgt gtcatccccg tacaggtctt tacaaaatgt gcacctgcct gcgaagcaag
     1201 cctgcatgca cgcatctttt cttcatcggt caaaagccct gtctcaatta tcactttaac
     1261 agtcctgtct tttgctgctt ctacaacctg acgaatatct tgcatgacat gatcatcttt
     1321 tccactcttc agcataccca cattgattac catgtcaaac tcatcacagc ccattttcaa
     1381 ggtttcctct gtttcaaaaa ccttggttga agttgttgtt gctcccaaag gaaaccctat
     1441 tacagtacac acctttacat ctgtaccact caaaagcctt ttgcatagag aaacataata
     1501 cgggtttaca caaacagaat aaaatccgta tttccttgcc tcctcacaaa gcctttcaat
     1561 atcaccaact gtggcagttg acttcaaatt cgtgtggtca atatatttgg caatatccat
     1621 ctgctctatc ctttctataa tatcatgctt aaaatctctt ctcttgaaag tgttgaaatc
     1681 aaggaaattg gcacatcgat tatggtatgg cagccaatac ttccttgttg tgacaattta
     1741 taagcagctc ttgcatatgc aaccagaata gcagaggtga attctgggtt tgactcaagt
     1801 ttaatggaaa actcaataat atgttgatta tcttccgaag tcttgccact ggttatgaca
     1861 aaaccaccat gaggaagctt tgcatggttt tccagaagct cttctttgga aacatactct
     1921 acaaaggtat cgtaatcagc aaaatatcca ggcatgttta caatttcttt tttgattttc
     1981 tccttgtcag cttcttcctt taccaccaca taacaatgcc tttcatgcat ttcccttttt
     2041 gtaaagtcgg gagtttctcc gtttcttaca cgattaacaa catcttctac aggaatagta
     2101 tattgtcttg catccaaaac accatctact cttctaactg catcagaatg accctgactc
     2161 actccaggtc cgtaaaatgt ataccctttt cc
//
LOCUS       DAONVY010000409         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_33655_length_2184_cov_3.271959, whole genome shotgun sequence.
ACCESSION   DAONVY010000409 DAONVY010000000
VERSION     DAONVY010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33655_length_2184_cov_3.271959"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLI11_09600"
     CDS             <1..1113
                     /locus_tag="PLI11_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HPZ53162.1"
                     /translation="GLDDIQAQRNTFRISSVCRTENANLSISDVIEIPSGKSPVLDLL
                     RTDARLSDVNFSVDDEKVQVKGYLNICALYVADDANQSTQVLENQIPFTQNLQIEDDE
                     PEVTYDVKTNIADFRADVVEDADGDKRMLALDANIDFVLSGYVTKECEVLTDAYSLTK
                     NFDLDYEVIDTPLKIGDVATQFVLKEVVSKSEEDPNMSEVINVNGTIGQADVIVEDGV
                     ATVEGFVFCNVLYLSDDPESPIASFTQQIPFTQTFDRNFDVDNPEVKAKLDVSHTSYS
                     ILSGQELELRIAISVRLDVACINHINTIVEARMPEEADKDFTNRPSILLYVVQPGDTL
                     WKVAKRYSAPLNILQNVNNLTNPDVLRPGQKLLIPT"
     gene            1257..1781
                     /locus_tag="PLI11_09605"
     CDS             1257..1781
                     /locus_tag="PLI11_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53163.1"
                     /translation="MSSKVKVVVIAIIGVFALSLILLGIRSALGTLKELDDGYDYKKA
                     DSYVKKIKKLCDAQDEFLVGDVFQFEYDRAYVFKDNYMSGESFARQYNLDISINQVEA
                     VDTEILRRIVFVDENGDYIYEFRYNVEKLTLMEEGMIIYPETIIKKEGIEPNGRCIIE
                     LVGNYKYYYDRNID"
     gene            1944..>2184
                     /locus_tag="PLI11_09610"
     CDS             1944..>2184
                     /locus_tag="PLI11_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53164.1"
                     /translation="MRIRLKPLLLVLIDILAVIIGYFLTLYIMFPLDNISVPSNINRL
                     IYSPASIVVIAIYILSFAIFEMYYTMLAHISIYDMF"
BASE COUNT          695 a          361 c          496 g          632 t
ORIGIN      
        1 ggtcttgatg acattcaggc tcagaggaat accttcagga ttagcagtgt ttgcagaaca
       61 gagaatgcca acctgtccat atccgacgtt attgaaatcc cctctggaaa gtctccggtt
      121 ctggatctgt taagaacgga tgcaaggctt tctgatgtga acttctctgt agatgatgaa
      181 aaagtccagg taaaaggata tttgaacatt tgtgccctgt atgtagcgga tgatgcaaac
      241 cagtcaacac aagtgttgga gaaccagatt ccttttacgc agaatctgca aattgaggat
      301 gatgaacctg aagttacata tgatgtaaag acaaacatag cggatttcag ggcggatgtg
      361 gtggaagacg cggatggaga caagagaatg cttgccttgg atgcgaacat agattttgtt
      421 cttagcggat atgtcacaaa agaatgtgag gtcctgactg acgcctactc acttaccaag
      481 aactttgatt tggactatga agtgatagac accccgttaa agataggcga tgtggcaacc
      541 cagttcgtac tcaaggaggt agtatccaaa tcggaagaag atccaaacat gtccgaggtc
      601 attaatgtta acggaaccat cggccaggcg gatgtgattg tggaagatgg agttgcaacg
      661 gtagagggct ttgttttctg caatgtactg tatctctcgg atgatccgga gtctccgatt
      721 gcatctttca cccagcagat tccattcact cagacctttg acagaaactt tgatgtggat
      781 aatccggagg tcaaggccaa actggatgtc agtcatacca gctacagcat tctttcagga
      841 caggaactgg aacttaggat tgctatcagt gtaaggcttg atgttgcatg catcaaccat
      901 attaatacaa tagtggaggc aagaatgccc gaggaagcag acaaggactt taccaacagg
      961 ccgtcaattc tcctgtatgt ggttcagccg ggtgacactc tgtggaaggt tgcaaagaga
     1021 tacagcgcac ctcttaacat cctgcagaat gtaaacaatc tgacaaatcc ggatgtgctg
     1081 agacctggtc agaaactact gattccgaca taagaaatcc aaacaaaatg attaacaaga
     1141 aaaagccgca aaaatccaca tggatttttg tggcttttat gttgattatt ttatagtacg
     1201 ctactataat tcaattaaca aaattattat ttcgcacaca tttgagagga agaatgatga
     1261 gtagcaaagt aaaggtagtg gttattgcca taattggtgt ttttgcttta tctttgattc
     1321 ttttaggaat tagatcagca ttaggcacac taaaagagtt ggacgatggc tatgattaca
     1381 aaaaggctga ttcgtatgtc aaaaagatta agaagctttg tgatgcccag gatgagtttc
     1441 ttgttggtga tgtttttcaa tttgagtatg acagggcata tgtctttaaa gacaactaca
     1501 tgagtggtga atcctttgcc agacagtata atctggatat ttccatcaac caggttgagg
     1561 cagttgatac tgaaatatta agacgaattg tttttgtgga tgaaaacggg gattacatat
     1621 atgaattcag gtataatgta gagaagttga cgttgatgga agaaggcatg ataatatatc
     1681 ctgaaacaat aataaagaaa gagggaatag aaccaaatgg cagatgtata atcgagttgg
     1741 ttggtaatta taaatactat tatgacagaa atattgattg aaaaaatgca cggaaaaagg
     1801 caggaacact gccttttttt gattgagaaa aattcattaa atgtgatata ctaatggaag
     1861 ccatttaatt tggcttgaca ataaagcatg catttgctat tgtaggaaaa gaaataattt
     1921 tgctgtagct ggagatgaat acggtgagga ttagattaaa accattactg ctagttctta
     1981 ttgacatact tgctgttata atagggtatt ttttgacatt gtacataatg tttccgctcg
     2041 ataatatatc ggtgccctca aatataaaca ggttgattta ctcacctgca tcgattgttg
     2101 taattgcaat atatatactg tcatttgcga tttttgaaat gtattacaca atgctggcgc
     2161 acatttccat ttacgacatg ttca
//
LOCUS       DAONVY010000410         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_33779_length_2178_cov_3.262365, whole genome shotgun sequence.
ACCESSION   DAONVY010000410 DAONVY010000000
VERSION     DAONVY010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33779_length_2178_cov_3.262365"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLI11_09615"
     CDS             complement(<1..593)
                     /locus_tag="PLI11_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53165.1"
                     /translation="MNIKRIRNSNFFRIFNLKDDLAKGRTSMLVSSIIVTSNTFLTAG
                     IFHTAFLIANDIDIVKVGIISFIPFLANTFSIFSPFILERFERRKGILTLGRLLYYTL
                     YILGVTLLPLVVKEQNAKVISFAVIVFISNVINALTVTGYAAWHVNFIPNKVRADYIS
                     YMQTLSYVFTGTVLIASSLIADKLRGSAYENTVLFSIR"
     gene            1030..1818
                     /locus_tag="PLI11_09620"
     CDS             1030..1818
                     /locus_tag="PLI11_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53166.1"
                     /translation="MYKYDERGNREESSPYDIEDLKLHEIYISMIDRIKKVVYSEGKK
                     EARSTFPSPDEIELTIDVEESLEVELGDKAILVPIDSVSFYLDGGRTLERVTNKNITL
                     RYDVDLDFAKDITWYLPSSIRNDVLWDLGLLEKNEEIDFRILIGLAAFGFFGLHAGCF
                     FVLRKCIPGFKYRHLVLFHLAHLFSVCLYLTVFPSIVTNMTMSYALAGLISKLSILGI
                     LPINLFFTFAFVGTAEKKIMPKLLIASSLILFVFYLVVVIIMNL"
     gene            1902..>2178
                     /locus_tag="PLI11_09625"
     CDS             1902..>2178
                     /locus_tag="PLI11_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014093520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HPZ53167.1"
                     /translation="MKVLYTAVVTNTGGRSGKAYSSDGKFEVNVATPVEMGGKDPDAT
                     NPEQLFAAGYSSCFNGALELVLRKNRVRFESTQVTASVSLLEDPEDNG"
BASE COUNT          679 a          345 c          485 g          669 t
ORIGIN      
        1 cgtattgaaa acaatactgt attttcatat gcagatcctc ttaacttgtc ggcaatcaga
       61 ctggatgcaa ttaatacagt gcctgtaaac acgtacgaca aagtttgcat atagcttatg
      121 tagtcggctc ttaccttgtt gggtatgaag tttacatgcc aggctgcgta tcctgttact
      181 gtaagggcgt ttatgacatt tgagatgaat acaataacgg caaaagatat tacctttgcg
      241 ttctgctcct ttacaaccag tggaagaagg gtaactccca gtatatacaa cgtgtaatac
      301 aaaaggcggc ctaaggtgag gatgcctttt cttctttcga atctctccag gataaagggt
      361 gagaaaatac tgaaggtgtt ggctaaaaag gggatgaaag agataatacc cacctttaca
      421 atgtcgatgt cgtttgcaat aaggaaagca gtatgaaaaa tacccgccgt caggaaggtg
      481 ttgctggtga caatgatgct tgaaacaagc atggatgtcc ttcctttggc caggtcatct
      541 tttaggttga atattctgaa aaaattactg tttcttatac gtttgatgtt caatcaaata
      601 catccttatg caatattttg agcatatttt aacaccaaaa aaaaaggaaa gcaatattgg
      661 tattctgggg aacaaaatca agacattttc aaatacatca atattcggtt gacagataat
      721 gtattaacat atattattta tctaatttga ctaagaacga gggaatatta tgaaaaagat
      781 atgtataatc ctgcttgcac tcatatcgtt atttctatgc gggtgtggta gagagcttaa
      841 acttacagga aaagaagtag tgaaagctga agtgtcgtat taaaagaact ggaacagaac
      901 aaggaaagca gcaggtatta tttatacttt ggaaatgacg agcaatatat tgagatattc
      961 cggcttgata atcttgttct atataaagat atcactgaaa aggaaaagtt cttttactac
     1021 gatggtacca tgtataaata tgatgaaaga ggtaaccggg aggaatcctc gccttatgat
     1081 attgaagatc taaaactgca tgagatttac atatcgatga ttgacagaat aaagaaggtg
     1141 gtgtattcag aagggaaaaa ggaggcaaga tcaacttttc cttctcctga tgaaatcgaa
     1201 ctgacaatag acgttgaaga aagcttagag gtagaacttg gggacaaggc aattttggtg
     1261 cctatagact ctgtttcatt ctatctggat ggtgggagga cacttgaaag ggtaacaaac
     1321 aaaaatataa ctctgaggta tgatgttgac cttgattttg caaaggatat tacatggtat
     1381 cttccttcaa gcattagaaa tgatgtattg tgggaccttg ggcttctgga gaaaaatgag
     1441 gaaattgatt ttcgcatact gattggtctt gcagcttttg gcttctttgg cctgcatgca
     1501 ggatgctttt ttgtgttaag gaaatgcatc cctggtttca agtacagaca tcttgtcctt
     1561 ttccatcttg cgcatctctt ttctgtctgt ctgtatctta ccgtttttcc ttcaatagta
     1621 accaatatga ccatgtctta tgccttggcg ggattgatat cgaaattaag tattctgggc
     1681 atattgccga ttaacctttt ctttacgttt gcatttgttg ggactgcgga gaagaaaatt
     1741 atgccaaaac tcttgattgc ctcaagtctg atattgtttg tgttctattt ggtggttgtg
     1801 ataataatga atttgtgaaa taaaaaaatt tgttagatga atctaatttt tcagaaaact
     1861 atggtataat aggttatagc atgaaaaagg aggagaacga catgaaagta ttatatactg
     1921 cagttgttac gaatacaggt ggtagaagcg gcaaggctta ttcatcggac ggtaaattcg
     1981 aagtaaatgt tgccacgccg gttgagatgg gtggcaagga tcctgatgct actaatccgg
     2041 agcagctttt tgcagcaggc tacagttcct gcttcaacgg agctttggaa ctggtgcttc
     2101 gaaagaacag ggtaaggttt gaatcaacac aggtgactgc atccgtttca ctattggaag
     2161 atcctgagga taacggct
//
LOCUS       DAONVY010000411         2174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_33872_length_2174_cov_2.672487, whole genome shotgun sequence.
ACCESSION   DAONVY010000411 DAONVY010000000
VERSION     DAONVY010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33872_length_2174_cov_2.672487"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLI11_09630"
     CDS             complement(<1..745)
                     /locus_tag="PLI11_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53168.1"
                     /translation="MGKRKVYIQNESKGHAIALMTVFILVLVIVAILSLLPGRSPKIG
                     DVDKNGVARSFLFPFVFIDEHKTLFILNDDLDVTEIDDNAHNAVHDVKKNKVYYIREN
                     TLYEYNIKKNLRVALAQDIDSYSILADRSGILLRSSNNLKLYMYSGKKTIELTEIKDI
                     NINNFSSIGDEYFLYIDNIQESEADLFLCSAIGKSRKIASNINIAKTFGIAKNDKYIY
                     YYIKSTLHIAERNGDVKLKLDNAQVLTNTN"
     gene            1041..1190
                     /locus_tag="PLI11_09635"
     CDS             1041..1190
                     /locus_tag="PLI11_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53169.1"
                     /translation="MISMYDGSYPWNLITKPIEYFAVSGKAFFTGLVEYFISYAINSY
                     INNLK"
     gene            complement(1195..2148)
                     /gene="metA"
                     /locus_tag="PLI11_09640"
     CDS             complement(1195..2148)
                     /gene="metA"
                     /locus_tag="PLI11_09640"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HPZ53170.1"
                     /translation="MPIKIPDNLPAKKILTKEHIFVLDEQMAIRQDIRPLRILILNLM
                     PTKIVTETQLLRMLSNTPLQTEVSFIHPASHTSKNTPPEHLSAFYSTFNEIKDEKYDG
                     MIITGAPVELLPFEEVNYWEELCAIMDWTTSNVMTTLHICWAAQAGMYYHFGIPKYPL
                     ENKLSGVFEHEPHKKSSRLLRGFDDTFYVPHSRYTETRISDVEKISDIDILATSREAG
                     LCICASKDRRQIFMTGHLEYDRDTLKLEYERDLAKGLNPNIPENYFPNDDPTQRPYHQ
                     WKSHAYLMYANWLNYYVYQATPYDLSNITKAFVPERKGEPE"
BASE COUNT          623 a          424 c          373 g          754 t
ORIGIN      
        1 ggttggtatt tgtaaggacc tgcgcattgt ccagttttaa tttcacatct ccgtttcgtt
       61 ctgcaatgtg caaagtgctt ttaatatagt agtaaatgta tttatcgttt ttcgcaatgc
      121 caaaagtctt tgcaatattt atattggaag caatctttcg tgattttcca atagcactgc
      181 acaaaaacag gtccgcctca ctttcctgta tgttgtcaat ataaagaaaa tactcatctc
      241 caattgaaga aaagttgttg atgtttatat ccttgatttc cgttaattct attgtttttt
      301 tgccagaata catatacagt ttcaagttat tactggaacg caaaagaatt ccggacctgt
      361 ccgcaagtat agagtacgaa tctatatcct gggctaaagc cacccttaaa ttcttcttaa
      421 tattatactc gtataaagtg ttttccctga tgtagtagac cttgttcttt tttacatcat
      481 gtaccgcatt atgggcgttg tcgtcaattt ctgttacatc aaggtcgtca ttaagaatga
      541 aaagcgtttt atgctcatct atgaatacga aaggaaagag aaagcttctt gcaacgccat
      601 ttttgtcaac atcccctatt ttgggagatc tgccaggcag caaacttaat atggccacta
      661 ttacaagaac aaggataaac actgtcatta aggcaatggc atgtccttta ctttcattct
      721 ggatgtacac ttttcttttg cccatagcac accttctatc aagaatttca cataaagtat
      781 aacattacca atggattttt gcaaatatcc attaatagtt attgcaatta ttaacgattc
      841 ggtttacaat agttttaaca gattatactg tgaaatattt tacaaaagag aatgatacaa
      901 aaatgagttt tgttaaaaga atgctggcat actatctata tattatccga tacttgtcac
      961 ttacaacatc aattatatat atgttgtctc ccattttatt catagtatta tatattcttg
     1021 cccaaaagga ttataatttt atgataagca tgtatgatgg aagctaccca tggaatctta
     1081 ttacaaaacc aatagaatac tttgccgttt caggcaaagc attctttact ggtcttgtcg
     1141 agtatttcat ttcctacgct attaactcat atataaacaa tttgaaatag tgtcttattc
     1201 cggctcccct tttctttccg gtacaaacgc ttttgttata ttgctcaaat cataaggagt
     1261 tgcctgatat acataatagt tgagccagtt tgcatacata aggtaagcat ggcttttcca
     1321 ctgatggtaa ggtctctgtg taggatcgtc atttgggaaa tagttttcag ggatattagg
     1381 attcaaacct tttgcaagat ctctttcgta ttcaagtttc aatgtatccc tatcgtattc
     1441 caaatgacca gtcataaata tctgccttcg gtccttggat gcgcatatgc agagtcctgc
     1501 ttccctgctt gttgctagta tatctatatc cgatatcttc tccacatcag atatccttgt
     1561 ttcagtatat ctagagtgag gcacataaaa agtatcgtca aatcccctta agagcctgga
     1621 tgatttctta tgaggctcat gctcaaatac tcctgacaac ttgttttcaa gtggatattt
     1681 gggtatgcca aaatgataat acatacccgc ctgcgctgcc cagcaaatgt gtaaggtagt
     1741 catgacattc gatgttgtcc agtccattat tgcacacaac tcctcccaat agtttacttc
     1801 ctcaaaaggc agcagttcga caggagcgcc tgtaattatc ataccgtcgt acttttcatc
     1861 ctttatctcg ttaaaagtac tgtaaaatgc agaaaggtgc tccggaggtg tgttcttgga
     1921 cgtatgggac gcaggatgta taaaagagac ctccgtctgc aatggagtat tgctgagcat
     1981 cctaagcagc tgggtctcag tcactatctt tgtaggcatc agattaagta taaggattct
     2041 caaaggcctt atatcctgtc ttatagccat ctgctcgtcc aatacaaata tatgttcttt
     2101 tgtcaaaatc ttttttgcgg gtaaattgtc gggtattttt attggcatat gttcactcca
     2161 tctttcttta tact
//
LOCUS       DAONVY010000412         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_33927_length_2171_cov_3.638941, whole genome shotgun sequence.
ACCESSION   DAONVY010000412 DAONVY010000000
VERSION     DAONVY010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33927_length_2171_cov_3.638941"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PLI11_09645"
     CDS             <1..992
                     /locus_tag="PLI11_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPZ53171.1"
                     /translation="NFKVDVKNRRATERNHSCTHLLHKALKMVLGEHVHQAGSSVNAK
                     RLRFDFNHMKALTKEELQMIEDIVNSHIDKGMEVKTDIMSMDEAMKSGAEALFDEKYG
                     DSVRVVSMGDFSKELCGGTHVKYTASIGLAKIVHEGAVAAGIRRIEVYTGSGAREYLV
                     NNLNILENISGTLKTAVHDTYAKVESLVEENKVLSREISELKKKIALGSSDDVLKNAK
                     DINGITVATGRFDMLDANSLRELADNLRDKMDAGFVLLASGLDDKVSIVCAATKSAVQ
                     KGVDCGRIVKEAASMCKGGGGGRKDMAQAGGKDASAIDKMLASVEDIFRKQLN"
     gene            1008..1352
                     /locus_tag="PLI11_09650"
     CDS             1008..1352
                     /locus_tag="PLI11_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPZ53172.1"
                     /translation="MADCIFCKIINGEAPAKKVYEDEYCVAFHDISPQMPVHIVVAAK
                     RHVASILEFKEEDKEFVGGIQLAIANIARELGITENGFRVINNCGKDALQTVPHLHYH
                     ILAGGKMGAKIV"
     gene            1372..>2171
                     /locus_tag="PLI11_09655"
     CDS             1372..>2171
                     /locus_tag="PLI11_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPZ53173.1"
                     /translation="MLLDDRIYFYDGSKGTMFIQSGFKDFPEALVLTYPDVVYELAKS
                     YVDVGCDFIQTNTFGANGHTLTLNNLANRQKEIITKAFELASKAASEREGCNVVFSIG
                     SIGKLMKPAGDMDFDEAYSLFYTMADYAFSAGARIFHLETFTDLYEMKVAVLALKDLS
                     EKKETKLAIFATVAIEENGRTLMGNTPEECARLLSKLGVDAVGANCSFGAEKMVPIIE
                     QMAKSTNLPIVAKPNCGLPRNVDGEIVFEQSPEEFAKECVALAKAGAQV"
BASE COUNT          672 a          371 c          573 g          555 t
ORIGIN      
        1 gtaatttcaa ggtggatgtg aaaaacagaa gggctactga gagaaaccac agctgcacgc
       61 atcttctcca caaggcgtta aagatggtat tgggagaaca tgtgcatcag gcaggttcca
      121 gtgtcaatgc caagagactc aggtttgact tcaaccatat gaaggctctt accaaagaag
      181 aacttcagat gatagaagat atagttaaca gccatatcga taaaggcatg gaggttaaaa
      241 ccgatataat gtccatggac gaggcaatga agtccggtgc tgaagccttg ttcgatgaaa
      301 agtacggcga tagtgtaagg gttgtaagca tgggtgattt ttcaaaagag ctttgtggag
      361 gaacccatgt aaagtacact gcaagcatag gtcttgctaa aattgttcat gaaggcgctg
      421 ttgcagcagg tataagaaga attgaagtat atactggaag cggtgctaga gagtatcttg
      481 taaataattt gaatatcctt gagaatattt cgggtacttt gaagactgcg gtacatgaca
      541 cttatgcaaa ggtggaaagc cttgttgagg aaaataaggt tttgtccagg gaaattagtg
      601 aactcaagaa gaaaatagct cttggttcca gcgatgatgt tcttaagaat gcaaaggata
      661 taaacggtat aaccgttgcg accggtagat ttgacatgct tgatgcaaac tccttgaggg
      721 agcttgctga caatttgaga gacaaaatgg atgcgggctt tgttctgctt gcctccggcc
      781 ttgatgacaa ggttagcatt gtttgtgcag ctaccaaatc agcagtacaa aaaggtgtag
      841 attgtggtag aatagtaaaa gaagcagctt ccatgtgcaa gggtggaggc ggaggacgca
      901 aggatatggc tcaggctggc ggcaaggatg caagcgctat tgataaaatg cttgccagtg
      961 ttgaagatat attcaggaaa cagcttaatt aatcaggagg gattgttatg gcagattgta
     1021 tcttttgcaa gataattaat ggagaggcac ctgcaaaaaa ggtgtacgaa gatgaatatt
     1081 gtgttgcatt ccacgacatc agtcctcaaa tgccagtaca catagttgtg gcggctaaaa
     1141 ggcatgttgc cagcattttg gagtttaagg aagaggacaa ggagtttgtg ggaggtatac
     1201 aactggcaat tgccaatatt gcaagggagc tgggcataac tgaaaacggc ttccgtgtaa
     1261 taaacaactg cggcaaggat gcactgcaga cagttcccca tcttcactac catatactgg
     1321 ctggtggaaa gatgggagcc aaaatagtat agttcaagag aggtggctgt tgtgctttta
     1381 gatgacagaa tatacttcta cgatggttcc aaaggaacca tgtttattca atctggtttt
     1441 aaagactttc cagaggctct ggtacttact tatccggatg tggtctatga gcttgcaaaa
     1501 tcgtatgtgg atgtaggttg tgattttatt caaacaaaca cttttggagc caatggccac
     1561 accctcacgc ttaacaatct tgcaaacagg cagaaggaaa tcataaccaa agcgtttgag
     1621 ttggcatcca aggcagcctc cgaaagggaa ggctgcaatg tggtcttttc gatagggtca
     1681 ataggcaaac tgatgaaacc tgcgggagac atggattttg atgaggcata cagccttttt
     1741 tacacaatgg ctgattacgc tttttccgct ggtgcaagaa tttttcatct tgaaaccttt
     1801 acggaccttt atgagatgaa ggttgcggtt ttggctttga aagatttaag cgagaagaaa
     1861 gaaacaaagc ttgccatatt tgcaactgtg gccattgagg aaaatggcag gaccttgatg
     1921 ggcaacacgc ctgaagaatg tgcaagactt ttgagcaaac tcggtgttga tgcagtagga
     1981 gcaaactgca gctttggagc agaaaaaatg gtgccgatta ttgagcaaat ggcaaaaagc
     2041 accaatcttc caatagtggc aaaacccaac tgcggacttc caaggaatgt ggatggagaa
     2101 atagtatttg agcaaagtcc tgaagaattt gcaaaagagt gtgtcgcgct tgcaaaggcc
     2161 ggggcgcagg t
//
LOCUS       DAONVY010000413         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_33984_length_2168_cov_3.336015, whole genome shotgun sequence.
ACCESSION   DAONVY010000413 DAONVY010000000
VERSION     DAONVY010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33984_length_2168_cov_3.336015"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLI11_09660"
     CDS             complement(<1..329)
                     /locus_tag="PLI11_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar hydrolase"
                     /protein_id="HPZ53174.1"
                     /translation="MHDKFLSKASELMPELNETIHRPVELVEALPDKNALHGVKMING
                     KYVENLYSQELQNGDIFTVDFGTHIVGYLTLKIKPVGHQQDSPLRLRLVFGEMPCEIP
                     DFEYAGTL"
     gene            506..1456
                     /locus_tag="PLI11_09665"
     CDS             506..1456
                     /locus_tag="PLI11_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPZ53175.1"
                     /translation="MKIGIPKGLLYYKYHPFFMTFFTELGAETIVSEDTNKSILDLGV
                     KHCVDEACMPVKVFHGHVASLRDKCDAILIPRIMQIRKREYICPKFCGLPEMVVYNIP
                     DLPAVISEPIYATDDKRLYEWAKEAGRLVTRDRNTIRRAFEKAVSVQNEYDIGINTLS
                     YEMKVALAGHPYNIYDSYSNMNIVRKLNALGIGIITEEFVDEEEVDSEVGKLFKRPFW
                     TFARNTYGFLATAYEKKLCDGVIYISSFACGIDSVLIELVKERIGDFPFMVLKIDEHT
                     GEAGVNTRIEAFVDMLERRKTYEDNVPSYGQCVPCSQGSF"
     gene            1398..>2168
                     /locus_tag="PLI11_09670"
     CDS             1398..>2168
                     /locus_tag="PLI11_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HPZ53176.1"
                     /translation="MKITFPHMGNVYLAVKALFDGLGIEYVIPPKNSKKTLEIGSLHS
                     PEEICLPYKIMIGNYVQAAEMGADTVLLTGSCGPCRFGEYCEMQMNLLNKLGHDFKFI
                     VIDAPGDIGVSELFNRIAEISTKSRMSKHKKLQCVKYALDVINLTDKLDAKAHWLAGY
                     ERKREECKKLLHASKRDAARCSNPKEMIKLLKDYEKKFDNVELDLDKDPVKIAIIGEI
                     YTVIEPFSNLYIEDKLMSYGVSTRRELTPSWWVKNMVMS"
BASE COUNT          689 a          368 c          501 g          610 t
ORIGIN      
        1 agagtgccag cgtattcaaa atccggaatc tcgcagggca tttcaccaaa gactagtctt
       61 agccttaaag gagaatcctg ctgatgtccc acaggcttta ttttaagtgt aagatatcca
      121 acaatatggg ttccaaaatc cacagtaaaa atatcaccgt tctgtagctc ctgggagtat
      181 aaattctcaa catatttgcc attaatcatt ttcaccccat gaagagcgtt tttatcgggc
      241 aaagcttcca ctagttcgac aggcctgtgg attgtttcat tcaattcagg catcagttca
      301 ctggcttttg ataaaaactt atcatgcatt catatcacct tttaataata atcctaacta
      361 ttatacatca tttttctctg ttttccacat tcaattgcga ccagtttcca tgactttttc
      421 agatgcatgg aataatttga ggaattgggg aaataatgaa aaggttgata tgtgaatata
      481 ttaagtaggt tgaaatgagg gttttatgaa aatagggata ccaaaaggtc ttttgtatta
      541 taaataccat ccttttttta tgactttctt tacggagctt ggagcagaaa caattgtctc
      601 ggaagacacc aacaagagta ttttggatct gggagtaaaa cattgcgttg acgaggcttg
      661 catgccggtt aaagtgtttc atggccatgt tgcttctttg agagacaagt gtgatgcaat
      721 attaattccg aggataatgc agattagaaa aagggagtat atatgtccca agttctgcgg
      781 gcttcccgag atggttgttt acaacatacc agatttgcct gctgtaatct cagagcccat
      841 ctatgcaacg gatgataaaa ggctttacga gtgggccaaa gaagcagggc gactggtgac
      901 cagggataga aatacaatcc gcagggcttt tgagaaggcg gttagtgttc agaacgaata
      961 cgatattgga atcaacacgt taagttatga aatgaaagta gcactggcag gacatccgta
     1021 caacatatat gacagttaca gcaacatgaa catagtaaga aaattaaacg cactgggaat
     1081 cgggataata acggaagaat ttgtggatga ggaggaagtg gattcagagg tggggaaatt
     1141 attcaaaagg cctttctgga cttttgccag aaacacttac ggatttctgg ctactgccta
     1201 tgagaagaaa ctatgtgatg gcgtaattta tatctcctcc tttgcatgtg gtatcgattc
     1261 cgtgttgata gagctcgtta aagaacgcat aggggatttt ccatttatgg tactaaaaat
     1321 cgatgaacac acaggagagg cgggagtaaa tacaagaatt gaagcttttg ttgacatgct
     1381 ggaaaggaga aagacgtatg aagataacgt tccctcatat gggcaatgtg taccttgcag
     1441 tcaaggctct ttttgatggt ctgggcattg agtatgtaat tccgcccaag aacagcaaaa
     1501 agactctgga aataggttcc ctacattctc ctgaggaaat atgtcttccg tataaaataa
     1561 tgattgggaa ttatgtgcaa gctgctgaaa tgggagcgga tacagtttta ttaactggga
     1621 gctgtggtcc ctgcaggttt ggagaatact gcgaaatgca aatgaatctg ttaaacaaac
     1681 tgggacacga ttttaaattc atagttatag atgcaccggg agatattgga gtatctgagc
     1741 tttttaatag aattgcggaa atatcaacga aaagcaggat gtcaaagcac aaaaagttac
     1801 aatgtgtaaa atatgctctc gatgttatta atctaactga caaactggat gccaaagccc
     1861 attggctggc aggttacgaa aggaaacggg aagagtgtaa aaaactattg catgcaagca
     1921 agcgggatgc agcaagatgc tcaaacccta aagagatgat aaagttactt aaggactacg
     1981 agaagaagtt tgacaatgtg gagttggact tggataaaga tcctgtcaag atagcgatta
     2041 ttggagagat ttacactgtc attgagccct tctcgaacct ttatatcgaa gacaagctta
     2101 tgtcgtatgg tgtttctacc aggagagaac ttactcccag ttggtgggtg aaaaacatgg
     2161 tcatgtcg
//
LOCUS       DAONVY010000414         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_34001_length_2167_cov_3.929451, whole genome shotgun sequence.
ACCESSION   DAONVY010000414 DAONVY010000000
VERSION     DAONVY010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34001_length_2167_cov_3.929451"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PLI11_09675"
     CDS             complement(<1..1041)
                     /locus_tag="PLI11_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ53177.1"
                     /translation="MIHTSPPDTFEYLIEYDEIEKFYKAYHEVRHDKEKLQKVLKMTG
                     DLRQYLLAEVFEEDLGELQVRTLFADRYKHDITIRKHTRYSPDLVHDHTFFEMVYILK
                     GKADNTFDNKLYKMSDGDICLIPPNVFHKLWVGDDSVVINIIMKKSLFDDNFLNEISK
                     GSMLIKFIKQNLYMHPLGEKKFLMFHALHNAPLRNLLAVLISESFKGRNSFPVKKALV
                     IAIFNYLANLEDYDDEVSEKNEVVHSILEYIQRNHATVTLSEVARHFNYSEPYFSKYI
                     KRLTGMSFVNILQTTRLKEACHLLATTNMRISDIPGKVGYDNVAYFNRVFKQKIGTTP
                     YKYRKMHRNAMLN"
     gene            complement(1120..>2167)
                     /locus_tag="PLI11_09680"
     CDS             complement(1120..>2167)
                     /locus_tag="PLI11_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPZ53178.1"
                     /translation="RLLNDIEERIDVEVEEDFLNQWRKFYNGQCKDEIFYPRRKKVSK
                     PKMDILGVNINDAINDHDLMLQYEFNNVSLNLGTPYNVLCVRANYGTGILSSLFGAEV
                     FVMPYEMKTLPTTRAINDTMKIREIVEKGVPDLKSGFGHKVFEMGEVYKEVMKDYPKI
                     SKYVHVYHPDLQGPLDICELLWGGGMFYAMYDDEELVHDMLSLITDTYIRFMDKWFEM
                     YHYEDNINAHWTCMFKGKVLLRNDSAMNISGEFYKEFAMPYDAKILDYYDGGIVHFCG
                     KGDHYIEYMSTIPKLYGINLSQPHYNDMERIYKHTVDKGLFIIELKRSRAEADVKREG
                     GFKGLVQTKEIPGW"
BASE COUNT          600 a          503 c          355 g          709 t
ORIGIN      
        1 attcagcatg gcatttctgt gcatcttcct gtatttgtac ggggtggtgc caattttttg
       61 cttgaacacc ctgttgaagt atgcaacatt gtcataaccc acctttccag gtatgtcgct
      121 aatcctcata ttcgttgttg caagcagatg acatgcttcc ttcaacctgg tagtctgcaa
      181 gatatttaca aacgacatcc cggtaagtct ctttatatat ttactgaaat acggctctga
      241 ataattaaaa tgccttgcca cttccgacaa agtgacagtt gcatggttcc tctgtatata
      301 ctcaagaata ctgtgtacta cttcattctt ctcagaaacc tcatcgtcgt aatcttccag
      361 gtttgcaagg taattgaata tcgctataac caaagctttc ttgacgggaa aactgtttct
      421 tcccttgaag gattctgaaa tcaaaacagc aagaagattc ctaagcggag cattatgaag
      481 cgcatgaaac ataagaaact tcttttctcc cagaggatgc atgtacagat tctgtttaat
      541 aaattttatg agcatgcttc ctttggatat ctcatttaaa aaattatcgt caaacagaga
      601 ttttttcatt attatattga taacaacgct gtcatcgccc acccagagct tgtgaaatac
      661 atttggagga atcaggcata tgtccccatc cgacatcttg tacagtttgt tatcaaatgt
      721 gttgtcagcc tttcccttta aaatgtaaac catctcaaaa aaagtatggt cgtgaactaa
      781 atcaggactg tatcttgtat gcttcctgat agttatgtca tgcttgtatc tatcagcgaa
      841 caaggttctt acctgcagct cccccagatc ctcctcgaaa acttccgcaa gaagatactg
      901 tctcaagtcg cctgtcattt tcagcacttt ttgaagtttt tccttatcgt gcctgacttc
      961 gtgatatgcc ttgtaaaatt tttcaatttc atcatattca ataagatact cgaaagtgtc
     1021 cggcggcgat gtatgaatca ttacaaaatc ctctctgtat ttttatatat tatatcctca
     1081 tcgaagaata tattcaaccg gacaaaagca ggatgactct caccaccctg gaatctcttt
     1141 cgtctggacc aatcctttga acccgccttc ccttttgaca tccgcttcag cccttgaacg
     1201 tttcagctcg attatgaaca gccccttgtc caccgtatgt ttatatatcc tctccatgtc
     1261 gttgtaatgg ggttgggaaa gatttattcc gtaaagctta ggtatggtac tcatgtattc
     1321 tatgtaatga tcgccttttc cacagaagtg tacaattcct ccgtcataat agtcaaggat
     1381 ttttgcatca taaggcattg caaactcctt gtaaaactcc ccggatatat tcattgcact
     1441 gtcatttcta agaagaacct ttcccttgaa catacaggtc cagtgagcgt tgatgttatc
     1501 ttcgtaatgg tacatttcaa accacttgtc cataaacctt atatacgtgt ccgttattag
     1561 tgacagcata tcatgaacca actcttcatc atcatacatt gcataaaaca tcccgcctcc
     1621 ccatagaagc tcgcatatat caagaggtcc ttgcaagtca ggatgataga catgcacgta
     1681 cttggatatc ttgggatagt ccttcataac ttccttgtat acttctccca tctcaaacac
     1741 tttatgtccg aacccgcttt ttaaatcagg cactcctttt tcaactatct ccctgatttt
     1801 catagtatca ttgattgcac gcgtagtcgg aagtgttttc atttcatatg gcatgacaaa
     1861 tacttctgct ccaaacagcg atgacaaaat accggttccg tagtttgcac gaacacaaag
     1921 cacattgtat ggagttccaa gattcaatga gacattgtta aactcatact gaagcattag
     1981 atcatggtcg tttatcgcat cattaatatt cactcccaga atatccatct tcggttttga
     2041 gacctttttt cttctcgggt agaaaatctc atccttgcat tgaccgttat agaatttacg
     2101 ccactggttt aaaaaatctt cctcaacctc cacatcgatt ctctcctcga tgtcattcaa
     2161 aagcctt
//
LOCUS       DAONVY010000415         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_34207_length_2157_cov_3.237869, whole genome shotgun sequence.
ACCESSION   DAONVY010000415 DAONVY010000000
VERSION     DAONVY010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34207_length_2157_cov_3.237869"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="PLI11_09685"
     CDS             complement(<1..1935)
                     /locus_tag="PLI11_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53179.1"
                     /translation="MKVRKVLILIMCILSFACGMLMAGADSSESKWIKLSSPYIVKEN
                     EGDIDYLYVVGGYHSIDSGTEIWGTANYSDIRLIGDKMGEIVITYENGTVDRVPLVFG
                     YTLWYYNNWKTFKAPFDGPEKDEKMANLLKEALHLYGAVEGNEKCVLKIKLRDEKVAR
                     IEIEDNKEKNGKPEIAGAYIVSGDVGQLTGGSVNVDTKVDFFEKYVIDSQNPYPDNVK
                     NAIEEIRKGLYTFDEDYAKEPIPYEYEKNHSGIKVRFYGNNLARIASGVFYHNLKDLS
                     ERVDENGLLHESYKDSPLYHDSFGTWQPGVAPYYNMFFTRNRSFTVLTAFGYKDLADR
                     AVGYANKKMMFFRENKLTIGGVQIPGHFTVTINDPMYYSETLVPIGWPTVYTKKAFGD
                     EYKNLGNQETDGHGLMMIGNYNVWKSLGKPREWVENNWVYINEACEWILWCFDNPKLS
                     FARKGLLFAESEGGMQEYTLYCNVPCYLGLLCYAEMADAIGKQDAAALWRKTAETMKE
                     GIESYILNTKYNKWSLHSGRIGFMHDPVLSMLADVYGFDKADIPQQDWLEYSLNTYPD
                     DIKSVSIYNYHGGVGGLGYNHCIMTQNALLTDHMADATKLVENLTKICYAPGKDKPYL
                     APEKFTYDSQNGLYLKNGDLG"
BASE COUNT          597 a          492 c          362 g          706 t
ORIGIN      
        1 gccaaggtct ccgtttttca agtacaggcc attttgggaa tcgtaagtaa acttctcagg
       61 tgccagatat ggtttgtctt tgcctggcgc atagcagatt tttgtgaggt tctcaaccag
      121 ttttgtagca tctgccatat ggtctgtaag aagggcattt tgtgtcataa tgcagtgatt
      181 gtatcccagc cctccaactc caccatggta attgtatatt gaaacagact ttatgtcatc
      241 agggtaggta tttaatgaat actcaagcca atcctgttgt ggaatgtctg ccttgtcaaa
      301 tccgtacaca tctgccaaca tggataatac agggtcgtgc atgaagccaa tcctgccgga
      361 atgcaaagac catttgttat acttggtatt aagtatgtat gactcaatac cctctttcat
      421 ggtttccgca gtctttctcc ataatgcggc agcatcctgc tttccaattg catcagccat
      481 ttcagcatag cagagaagtc caagatagca tggtacattg cagtacaaag tgtactcctg
      541 cataccacct tccgactctg caaaaagaag tccttttctt gcaaaagaca gtttgggatt
      601 atcaaaacac caaagtatcc attcacaggc ttcatttatg taaacccagt tattctccac
      661 ccattccctg ggtttaccta acgatttcca gacattgtag ttgccaatca tcatgagtcc
      721 atgaccatca gtttcctgat tgccaagatt cttgtattca tcaccaaaag ccttttttgt
      781 gtatacagtt ggccatccta ttggcaccag tgtttcagaa tagtacatgg gatcatttat
      841 ggtaactgta aagtgaccgg gaatttgaac tcctccgata gtcagtttgt tttctctgaa
      901 aaacatcatc tttttattcg cataaccaac agccctgtcg gcaaggtctt tgtacccaaa
      961 ggctgtaaga acagtgaaac tcctgttgcg ggtgaaaaac atgttgtaat agggggcaac
     1021 tcccggctgc caggtcccga aactgtcatg gtacagtggt gaatccttgt atgattcatg
     1081 caaaagtcca ttctcgtcga ctctttcaga caggtccttc aagttgtgat aaaatactcc
     1141 gcttgctatt ctggcaaggt tgttgccgta aaaacgcact tttattccag agtggttttt
     1201 ctcatattca tatggtattg gttcttttgc ataatcctca tcaaatgtat acaaaccctt
     1261 ccttatttcc tcgatggcat tttttacatt gtctggatat ggattctgcg agtcaatcac
     1321 atacttctca aaaaaatcaa cctttgtatc tacattaaca ctgcctcctg ttagctgccc
     1381 aacatctccc gacactatat acgctcctgc aatttcgggt ttgccgttct tttccttgtt
     1441 atcttctatc tcaatccttg caaccttttc atcccttaac tttattttta gaacgcactt
     1501 ttcattacct tcaacagctc cataaagatg taatgcctct ttgagcagat ttgccatttt
     1561 ttcatccttc tcaggtccgt caaaaggtgc cttgaaggtt ttccagttgt tgtaatacca
     1621 aagagtatat ccaaacacga gtggaaccct gtccaccgtg ccgttttcat aagttattac
     1681 gatttccccc attttgtctc caatgagtct tatatcgctg taattggcag taccccagat
     1741 ttctgttcca ctgtcaatgg aatgataacc tcccaccaca tacaaatagt caatatcccc
     1801 ctcgttttct ttcactatat atggtgaact caattttatc catttactct cggatgaatc
     1861 agctcctgcc ataagcatgc cgcaggcaaa tgacaatata cacataatca atatcaaaac
     1921 ctttcgaact ttcaaaatat cactctccat tctgactaac agatagtata attctataaa
     1981 tttaaaagta ttattccagt tcattatgta caaatccata tacaattcag attttttatc
     2041 tattcttaac aaatgcaagt gtttgagcca agtcatgcta ttatcactca ttattaaatt
     2101 tattaaaatt taggtaagca taaaaaatat ggaggaatca gttatgagaa gaataat
//
LOCUS       DAONVY010000416         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_34549_length_2141_cov_3.824065, whole genome shotgun sequence.
ACCESSION   DAONVY010000416 DAONVY010000000
VERSION     DAONVY010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34549_length_2141_cov_3.824065"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLI11_09690"
     CDS             complement(<1..491)
                     /locus_tag="PLI11_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HPZ53180.1"
                     /translation="MIELIKDYVKMTKVILVPPSRSVYEHDCIVPDALPDMLKVLVAD
                     GVSTVDRVSVMKDQVTVDFSIKYRILYLADNAEASIKSFSTMANHSATFDAPEVDSNT
                     ECRVECIIEHTEHTFINSRKLAFRTVVKIEPELYNTEEKGITAGLDGLDDIQAQRKTF
                     RIS"
     gene            complement(567..1643)
                     /locus_tag="PLI11_09695"
     CDS             complement(567..1643)
                     /locus_tag="PLI11_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53181.1"
                     /translation="MMTSDDVRIIEEAYGIKVDRLVQTRDSYNITAKDASRYILRPIK
                     GPPARTYYTFIAVEHLKNNGFGNVCSYIKNKHDMPFVRLSGGFYTLTPVIQGCEMSFD
                     NYKHLSEASYMLGRMHIASKGFNLEYAREKMLKGVCSIDGLKMAEFGDGKLIEDKVNL
                     WVRCSIGQTPGLFKKRLNDLIRYKKVALRNRGKFELEYLLVCDNYIALAERVIQDLEA
                     SAYDEIVKEACLEGTICHRDYTAHNIITGPMGDFVSNFDFCAIELPVYDVANFLRRKL
                     RKSHWSINDAKFILDHYNSVRPMSKDEMYILKLLLMFPQKFWRNVNRYYNSRKSWCEK
                     SCIEKLKEIEEERKSIEKILSIFD"
     gene            complement(1734..>2141)
                     /locus_tag="PLI11_09700"
     CDS             complement(1734..>2141)
                     /locus_tag="PLI11_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53182.1"
                     /translation="SLYLISTDIDKLLEPGLSMYADKHYSCDARKVDLGDKKVDAVVT
                     EIPFSENYTPVAIEAFYNLLPNLSDNGKLLVMCHVGQTEQIKKKMEKSNLCLIVDKEV
                     NRKGTSVNILLWSKCREFYKKNLKYFQMIKTVI"
BASE COUNT          588 a          480 c          347 g          726 t
ORIGIN      
        1 ctgcttatcc tgaatgtttt cctctgtgcc tggatgtcgt caaggccatc aagtcctgca
       61 gtgattcctt tctcctccgt attgtacagc tcaggctcaa tcttcaccac tgtcctgaaa
      121 gccagttttc tactgtttat gaatgtgtgc tccgtatgct ctatgatgca ttcaaccctg
      181 cattcggtat tggaatcgac ttcaggggcg tcaaaggttg ctgagtgatt tgccattgtg
      241 gagaaggatt tgattgaagc ctccgcatta tccgccaaat acagaattct gtattttata
      301 ctgaaatcca ctgtcacctg atctttcatt acagatactc tgtccactgt tgaaacaccg
      361 tctgccacaa gtaccttcaa catgtcaggc aaggcatctg gaacgatgca gtcgtgctca
      421 tatacagaac ggcttggggg taccaaaatc acctttgtca tttttacata atcttttatc
      481 aactctatca ttatccgtcc tcctagctga gtattttcat gggtttgtaa atttatatgc
      541 cgagaaaaat attcttatgc atcctctcag tcaaagattg aaagtatttt ctcgatactc
      601 tttctttcct cttcaatttc cttaagtttc tcaatacagc ttttttcaca ccagctcttc
      661 cttgagttgt aatacctgtt tacattcctc caaaacttct gtggaaacat aagtaaaagc
      721 tttagaatat acatttcatc cttggacatt ggccttacag agttgtaatg atcaaggatg
      781 aatttcgcat cattgattga ccagtggctt tttctaagtt ttcttctcaa gaagtttgcc
      841 acatcataaa ctggtagctc aattgcacaa aaatcaaaat ttgacacaaa gtcacccata
      901 ggccctgtaa ttatattgtg ggccgtatag tctctgtggc atattgtacc ttccaaacag
      961 gcttccttaa caatttcatc gtaagcggac gcttccaaat cttgaataac cctttctgca
     1021 agtgctatgt aattgtcaca aaccagaagg tattcaagct caaactttcc tctgttcctt
     1081 aaagcaacct tcttgtacct tatcagatca ttcagtcttt ttttgaaaag gcctggcgtc
     1141 tgtccaatgg aacaccttac ccacaaattc actttatcct caatgagttt tccgtctcca
     1201 aactcagcca tcttcaagcc atcaattgaa caaacaccct taagcatctt ttctcttgca
     1261 tattctaaat tgaatccctt ggatgcaata tgcattcttc caagcatgta tgatgcttcc
     1321 gaaagatgct tgtaattgtc aaaactcatt tcacaacctt gaataacggg agtaagtgtg
     1381 taaaaaccac cgcttagtct gacaaaaggc atgtcatgct tgttttttat gtaagaacaa
     1441 acattgccaa atccgttgtt ttttagatgt tccactgcaa taaatgtgta gtatgtcctt
     1501 gcaggtggtc cctttattgg ccttagtatg tatctggaag cgtcctttgc tgtaatgttg
     1561 tacgagtctc ttgtctgtac taatctgtca accttgattc cgtaagcctc ttctattatt
     1621 ctaacatcat ccgaagtcat cattttaccc ccttatagaa atgacagcta gaataatata
     1681 tgtacaaaac tattcatgta tgacagatta gacaatcaag taatatgatt ttgtcatatg
     1741 actgttttaa tcatttggaa atatttcaag ttctttttat agaattctcg acatttggac
     1801 cataacaaga tatttactga agttcctttt ctgttgactt ccttgtctac tatcaagcaa
     1861 agattgcttt tctccatttt ctttttaatc tgttccgttt gcccgacatg gcacataacc
     1921 aaaagcttgc cattgtcaga taaattggga agaagattgt aaaaagcttc aattgcaacg
     1981 ggtgtgtaat tttctgaaaa aggtatttct gtaaccaccg catccacctt tttgtcgccc
     2041 aaatcaactt ttcttgcatc gcacgaataa tgcttgtctg catacatcga cagtccaggc
     2101 tccaacaact tgtcaatatc agtactaatc aaatacagcg a
//
LOCUS       DAONVY010000417         2136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_34658_length_2136_cov_2.684286, whole genome shotgun sequence.
ACCESSION   DAONVY010000417 DAONVY010000000
VERSION     DAONVY010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34658_length_2136_cov_2.684286"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLI11_09705"
     CDS             complement(<1..507)
                     /locus_tag="PLI11_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPZ53183.1"
                     /translation="MLEKNIHTFIGADKGYKESDICIYGCPFDGTTSYRPGARFAPSF
                     IRNESYGIETYSPYQNLDLTDFNFFDGGDLEIPFGNTQKVLDMTKEYALKILRDKKLP
                     FLIGGEHLVTLAQVRACTEIYSDLHIIHFDAHCDLRNEYMGEKLSHATVMRRCFELVG
                     ENRIFQFGI"
     gene            complement(501..1349)
                     /gene="speE"
                     /locus_tag="PLI11_09710"
     CDS             complement(501..1349)
                     /gene="speE"
                     /locus_tag="PLI11_09710"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPZ53184.1"
                     /translation="MELWFSEEHTDYAKFSIKIKQQLESAKSEFQDIAVFDTYEFGRI
                     LVLDGLLMLTERDEFVYHEMITHVAMAVNPRIKDVLVIGAGDGGTVRELARYEHIERI
                     DMVEIDKMVVDVCKKHIPQTACCFDDKRVNLYFEDGVKYVRRVVDEYDLIIVDSTDPF
                     GPGEALFTKEFYGNCFKALKEDGILVNQHESPYYEGDRAAMQRSHKLIKSIFPISKVY
                     QAHIPTYPSGHWCFGFASRTLDPIEDLKAEEWKRFNLETRYYNTELHAGAFYLPTYVK
                     ELLEKC"
     gene            complement(1352..>2136)
                     /locus_tag="PLI11_09715"
     CDS             complement(1352..>2136)
                     /locus_tag="PLI11_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPZ53185.1"
                     /translation="VSMHKTGGSLTQSSLLLCGPDVDANYVRQVLNLTQTTSGSYLLM
                     MSLDVARRNLALRGREMIAKTVEYAEYARKEIDQIGGYYTFSSDLVNGDTVYAYDNTK
                     LTVHTRNIGLAGIEVLNILRDEYGIQLEFGDMGNILAIISAGDRYQDIERLISAMSEI
                     KRVYAKDTEEVFDCDYIVPNVQITPQKAFYAEKRTLPIEESVGRICSEFIMTYPPGIP
                     ILAPGELITKEILEYIAYAREKGALLTGTQDMTLQTINVVEG"
BASE COUNT          581 a          525 c          361 g          669 t
ORIGIN      
        1 aatgccgaac tgaaagattc tgttttcccc aaccagttca aaacaccttc tcatgacagt
       61 tgcatgggaa agcttttccc ccatatactc attccttaaa tcgcagtggg catcaaagtg
      121 gattatatgc aaatcagaat atatctctgt acaagccctt acctgggcca gggttaccaa
      181 atgctctcca ccaataagga aggggagttt tttgtctctt aaaatcttaa gagcgtactc
      241 tttagtcata tccaaaacct tttgggtatt accaaagggt atttcaagat ctcctccgtc
      301 aaagaaattg aaatctgtca aatccaaatt ctgatatgga ctatacgttt caatcccata
      361 gctttcattt cttataaaag aaggggcaaa tcttgcccct ggtctgtaag atgtcgttcc
      421 gtcgaacgga cacccatata tgcaaatatc gctttcctta taccctttgt ccgcaccaat
      481 gaaggtgtgt atgttcttct ctaacatttt tcaagcaact cctttacata agtaggaaga
      541 tagaaggctc ctgcatgaag ctcggtgttg taatatctgg tttcaagatt gaacctcttc
      601 cactcctctg cctttaaatc ctcgattgga tccaaagtgc gggaagcaaa tccaaaacac
      661 cagtgacccg agggatatgt gggtatgtga gcctggtaaa ccttgcttat tggaaatatg
      721 gattttataa gtttgtgaga cctctgcatt gctgctctgt caccttcgta ataagggctt
      781 tcatgctggt tgacaagaat tccatcctct tttaaagctt tgaagcagtt gccgtaaaac
      841 tcttttgtaa acaaagcctc tcccggcccg aaaggatctg tagagtcgac aattatcaag
      901 tcgtattcat caaccactct tcttacatat ttaactccat cctcaaagta gagattcact
      961 ctcttatcgt caaaacagca ggcagtctgg ggaatgtgct ttttacaaac atcaaccacc
     1021 atcttgtcta tctccaccat gtctatcctt tcgatatgtt catatctggc aagctccctt
     1081 actgttccgc catcacctgc gccaataacc agtacatcct ttattctagg attaaccgcc
     1141 attgccacat gggttatcat ttcgtgataa acaaactcat ccctttcagt cagcatgagc
     1201 agtccgtcaa gtacaagtat tctcccaaat tcataagtat cgaaaactgc tatatcctga
     1261 aactcacttt tcgcactctc aagctgctgc tttatcttta ttgaaaattt agcatagtcg
     1321 gtgtgttctt cactaaacca aagttccatc attacccctc cacaacattt attgtttgca
     1381 atgtcatatc ctgcgttcct gtcaaaagcg cacccttttc cctggcatag gcaatatact
     1441 caagaatttc ctttgtaatc aattcacctg gagccagtat gggtattccc ggcggataag
     1501 tcataataaa ttcagagcag atcctgccta cactttcctc aataggcaac gtacgtttct
     1561 ccgcatagaa tgctttttga ggtgttatct gtacatttgg aactatatag tcgcaatcaa
     1621 acacctcttc cgtatctttt gcatatactc tctttatctc agacatagcc gatattagtc
     1681 gttctatatc ctgatacctg tcccctgcag atataattgc cagaatgttt cccatgtcgc
     1741 caaattccaa ctgtatgccg tattcgtccc ttagtatgtt gagcacttca atacctgcaa
     1801 gtcctatgtt tcttgtgtga accgtcagct ttgtgttgtc ataggcataa acagtatcgc
     1861 cattaacaag atcacttgaa aaggtatagt atccacctat ctgatctatt tcctttctgg
     1921 catattctgc atactccaca gtttttgcta tcatttccct gcctctcaag gcaagattcc
     1981 ttctggctac gtctagagac atcataagta gataagaacc gcttgtggtt tgagtcagat
     2041 tcaaaacctg ccttacataa tttgcatcca catccggccc acacaacaaa agcgagcttt
     2101 gtgtcaaaga accacctgtt ttgtgcatgc tgactg
//
LOCUS       DAONVY010000418         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_34851_length_2128_cov_2.951278, whole genome shotgun sequence.
ACCESSION   DAONVY010000418 DAONVY010000000
VERSION     DAONVY010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34851_length_2128_cov_2.951278"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLI11_09720"
     CDS             complement(<1..313)
                     /locus_tag="PLI11_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53186.1"
                     /translation="MKLLIMSYSYTGNNEIFAKSIAEKLNMEHICITENKKRTIGTII
                     ADFVLHRTPQTEPSPQVMSDYDLIIFVAPVWMGQPAFPLRPYFNELKKHPQKYAYISL
                     SG"
     gene            complement(343..1383)
                     /locus_tag="PLI11_09725"
     CDS             complement(343..1383)
                     /locus_tag="PLI11_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HPZ53187.1"
                     /translation="MALVTIVGSGMMGSALAFPARENGNEVRIVGTHLDREIINRCKI
                     ENKHPKFNKEFPEGIDFYQIEELESALLGTDVVICGVSSFGVTWFAEKVLPVLPENVK
                     MLAVTKGLIDSPDGKLITYLDFWKDKLNELGKNVSLNAIGGPCTSYELVAQDQTVVAF
                     CGENMADLKYLKEVLQTDYYHIRLSTDITGIESAVALKNAYALGIALTIGLNQRVFGL
                     DSEIHFNSQAAVFGHAVKEMYRLLEFQNALTLDNLCVGVGDLYVTVYGGRTRMIGILL
                     GRGLDIDEAKAELDGVTLESLVISERVARVVKLNIEKGILKKEEFPLLLHIDEILTEK
                     KAVNIPWDSFTL"
     gene            complement(1455..>2128)
                     /locus_tag="PLI11_09730"
     CDS             complement(1455..>2128)
                     /locus_tag="PLI11_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53188.1"
                     /translation="YIMQGGYFDGEKFYVAMIKKDSQGYELTKIHVLDKKGKIIKVSD
                     ILELDHANNITYNSKKKMFLVSHCQSPDDHYYRYSFVDPDTFEIIETADLPKPFFAMA
                     YSEGRDMYASGEWAGETLDIWDGDLNLVKSVSVEAPGSLSQGVYCDYEYIYFVRSSQN
                     GYQAEIRVYDFDCNLVWAIPLDVPDGVEPENINIVKGKTYIMGNDWKNGCGVVYVVHF
                     EEVSK"
BASE COUNT          624 a          483 c          332 g          689 t
ORIGIN      
        1 atccacttaa ggatatgtat gcatattttt gaggatgttt ctttaactca ttaaagtatg
       61 gccttaaagg aaatgcgggt tgccccatcc atactggtgc gacaaatata attaaatcat
      121 aatcagacat aacttgtgga cttggctctg tctgtggtgt cctgtgtaaa acaaagtctg
      181 ctattatagt accgatggtt cgtttcttat tctcggttat gcatatatgt tccatgttta
      241 acttttcggc aatactttta gcaaaaatct cgttattgcc tgtgtaggaa taggacataa
      301 ttaataactt cataaataca cactcctgtt tagaaaaaat atttataatg tgaagctatc
      361 ccaaggaata tttaccgcct ttttctcagt aagtatttca tcaatgtgta acagcagcgg
      421 aaattcctct ttctttaaaa ttcctttctc aatattcaac tttactaccc ttgcaactct
      481 ttcagatatg accaatgatt ccaatgtcac tccatctaat tctgccttgg cttcgtcaat
      541 atcaagtcct cttccgagta aaatgccgat cattcttgta cgtccaccgt aaacagtaac
      601 atataaatcg cccacaccta cacacaaatt gtccaaagtc aaggcattct gaaattccag
      661 caaacgatac atttccttta ctgcatgacc aaagactgcc gcctgtgaat taaaatgtat
      721 ttcactgtcc aaaccaaaca ccctctgatt caatccgatt gttaatgcaa ttcccaaagc
      781 gtatgcattt tttagggcaa ctgcactctc gatgccagtt atatccgttg aaagacgaat
      841 gtggtaatag tctgtctgca atacttcctt taaatactta aggtcggcca tgttttctcc
      901 acaaaaagca actactgtct gatcttgtgc taccagctca taactggtac atggtccacc
      961 aatcgcattt aaagaaacat tcttgccaag ttcgttcagt ttatccttcc aaaaatcaag
     1021 atatgttatc agtttgccat cagggctatc tataagaccc tttgttacag caagcatttt
     1081 cacattctct ggcaaaactg gcagtacttt ttctgcaaac cacgtcactc caaaactact
     1141 cacgccgcag ataacaacat cagttcccaa aagggcactt tccagctctt ctatctgata
     1201 aaagtcaatt ccttcaggaa actctttgtt aaattttgga tgtttgtttt ctatcttgca
     1261 gcggttgata atttctctat cgagatgtgt tccaacaatt cttacctcat tgccattctc
     1321 ccttgcaggg aacgcaagtg cagaacccat catgccagaa cctactattg ttactaatgc
     1381 catttcatca ccatctaatt tatattttac aaatattgta gaatcagctg aaaactatgt
     1441 caataaagtt atattcattt tgacacttct tcaaaatgaa cgacatatac cacaccacag
     1501 ccgtttttcc agtcattacc catgatgtat gttttacctt taactatatt aatattttca
     1561 ggttcaactc cgtcaggcac atccaaaggt atggcccaaa ccaaattgca atcaaagtcg
     1621 taaacgcgta tttctgcttg gtatccgttt tgcgaggacc ttacaaagta gatatactca
     1681 tagtcgcaat aaactccttg ggaaaggctt cccggtgcct ctaccgatac ggattttaca
     1741 agattcagat ccccatccca tatatccaga gtttctcctg cccattcacc ggaagcatac
     1801 atgtctctgc cttcactgta tgccatggca aagaaaggtt tgggcagatc cgcagtttca
     1861 ataatttcaa aggtgtctgg atcaacaaag gaatatctgt aataatgatc atcgggggac
     1921 tgacagtgag atacaagaaa cattttcttc tttgagttgt atgtaatatt gtttgcatga
     1981 tccaattcca gaatgtcact taccttgatt atcttaccct tcttgtcaag aacgtgaatt
     2041 ttagtaagtt catatccttg cgaatctttt ttaatcatgg ctacatagaa tttctcgccg
     2101 tcgaaatatc cgccctgcat tatatagt
//
LOCUS       DAONVY010000419         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_34894_length_2126_cov_2.636890, whole genome shotgun sequence.
ACCESSION   DAONVY010000419 DAONVY010000000
VERSION     DAONVY010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34894_length_2126_cov_2.636890"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PLI11_09735"
     CDS             <1..878
                     /locus_tag="PLI11_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53189.1"
                     /translation="AIEKDPPMIFICGWNEWIAGNWDSDRGWSKDNQLGKYTDDPRDM
                     CMFVDNFNIDHSRDIEPMTGGYGDNYYMQMCNFIRQYKGTNGRVNVGKKTTVDINGDW
                     SQWDDATAVYKDFINDTSIRDVSLYDKWKNIDISEYENNTGRNDISVMKVARDDKHFY
                     FYVDTVEDISDPTDNWMTLFLATGFSKTKKWNGYDFVVNRTAPPSKNKAVLEKYAGDN
                     KWEKVAEVDMRVEGNKLMLKVPASKIGISSMDIVNLQFKWADNCNMDNGIWTFYQDGD
                     AAPYGRFNFLFSNKA"
     gene            976..1824
                     /locus_tag="PLI11_09740"
     CDS             976..1824
                     /locus_tag="PLI11_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ53190.1"
                     /translation="MFVQVYNENNWEELDYVSDDYLYINCSGCYEIGEKDMTTIRPKG
                     RKDYQLIYFWRGKGLFYHNNEEIEVNAGEVFLFKPYEPHNYVYVGGMDTLVYWVHFTG
                     SAVEDLLRKNNLTSGVVYHVGICVEITEMFNNLINEIKYRKPAYNSLLNAFFLTLVSL
                     VKRRIIEYRDELFSSYSTMLQPVIDDINKNYANNKTIEDYAEMCHLDKYYFIRCFKKH
                     TGMTPYAYKIQVRMKNAKHLLSNTSMSIKEIAEMVGFSDQLYFSRTFKKYSRLTPSEY
                     RRQSML"
     gene            1933..>2126
                     /locus_tag="PLI11_09745"
     CDS             1933..>2126
                     /locus_tag="PLI11_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53191.1"
                     /translation="MNKSDWQDMSLLHRNRLKERAYFIPYESTSSALTKDKYKSKWYK
                     TLNGVWDFKYYDAWYRESDN"
BASE COUNT          724 a          307 c          475 g          620 t
ORIGIN      
        1 atgcaattga gaaggatccg cctatgatat tcatctgcgg atggaatgaa tggattgcag
       61 gcaattggga ttcggacaga ggatggagta aagataatca attaggaaaa tatacggacg
      121 atcctcgtga tatgtgcatg tttgtggaca atttcaacat tgaccacagc agggacatag
      181 aacccatgac aggtggttat ggggacaatt attacatgca aatgtgcaat tttatcaggc
      241 aatacaaagg aacaaacggc agggtaaatg ttggcaagaa aacaacagtt gacataaatg
      301 gcgactggtc tcaatgggat gatgctacag cagtctacaa ggacttcata aatgatacga
      361 gcattagaga tgtatccttg tatgacaaat ggaaaaatat tgatatatca gagtatgaaa
      421 acaacacagg aagaaatgac ataagtgtca tgaaagtagc aagggatgac aagcacttct
      481 acttttatgt agatacagta gaggacatat cagaccctac ggacaactgg atgacgctgt
      541 ttcttgccac tggtttttca aaaacaaaga agtggaatgg ctatgatttt gtcgtaaaca
      601 ggactgctcc cccgtcaaag aataaggcgg ttttggaaaa atatgcagga gataacaagt
      661 gggaaaaggt agcagaagtt gatatgaggg ttgaaggaaa caagcttatg ttgaaagttc
      721 ccgcttcaaa aattggcatt tcgtcaatgg acatagtgaa tcttcaattc aagtgggcgg
      781 ataactgcaa catggataat ggaatatgga ccttctacca ggatggtgat gctgcaccat
      841 atggcagatt taacttcctg ttcagcaata aagcgtgatt gcatttagat ggctgcttgt
      901 atggcagcct ttatcttttg gctgacaaat tgtataataa acgtaatggt atctgctatg
      961 taggagagga cgtatatgtt tgtgcaagtg tataatgaga ataattggga agagctggat
     1021 tatgtatcgg atgattattt atatataaat tgtagtggtt gttatgaaat aggggaaaag
     1081 gacatgacaa ccataagacc aaaaggcaga aaagattatc aacttattta tttttggaga
     1141 ggcaaaggtc tgttttatca taataatgaa gaaattgagg taaatgctgg tgaggtgttt
     1201 ttgtttaagc cttatgaacc tcataattat gtatatgtgg gaggtatgga cacccttgta
     1261 tattgggttc attttacggg tagtgcagtt gaagatttgc taagaaaaaa caatcttacc
     1321 tctggtgttg tatatcatgt tggtatctgt gttgaaataa cggaaatgtt taataatttg
     1381 ataaatgaga ttaaatacag aaagccagca tacaactctc tattaaatgc attttttctt
     1441 acccttgtat ctcttgttaa aaggagaata atcgagtata gggatgagtt gttctccagt
     1501 tattccacta tgcttcagcc ggtcattgat gatattaaca aaaactatgc aaataacaag
     1561 accattgaag attatgctga gatgtgtcat ctggacaaat actattttat cagatgtttt
     1621 aaaaaacaca ccggcatgac tccctatgcg tataaaattc aggtgcgcat gaaaaatgca
     1681 aagcacctcc tttccaatac ttccatgagt attaaggaaa ttgcagaaat ggtaggtttc
     1741 tcagatcaac tctatttcag tcgaacattc aagaaatatt ctagattgac gccttctgaa
     1801 tatagaagac aaagcatgtt gtaaaccaag agtacaattt gttccatgta tatcccaata
     1861 agttctatgt ttttatttgc cttggctgat ataattagga cacatgtgaa aaaggcagag
     1921 aggagtgctg taatgaataa aagtgattgg caagatatga gtcttttgca taggaacagg
     1981 ttaaaagaac gtgcatactt tattccttat gaatcaacta gttcagcttt gacaaaagac
     2041 aaatataagt cgaagtggta taagactttg aatggtgtat gggactttaa atactatgat
     2101 gcctggtatc gtgaaagtga taataa
//
LOCUS       DAONVY010000420         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_35317_length_2108_cov_3.084267, whole genome shotgun sequence.
ACCESSION   DAONVY010000420 DAONVY010000000
VERSION     DAONVY010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35317_length_2108_cov_3.084267"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLI11_09750"
     CDS             <1..410
                     /locus_tag="PLI11_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPZ53192.1"
                     /translation="ESFNKNEYVEKVINDIIYNFSDPDFDINEALDKTHLNKDYFRRL
                     FVKHTGKTPLQFLTSKRITFAKQLLSTQKQTDMQICEIAWRCGFSNCYYFSRVFKKET
                     GMSPKAWADSYANEKNIDTEHNEPIPTRYFTPQ"
     gene            complement(414..>2108)
                     /locus_tag="PLI11_09755"
     CDS             complement(414..>2108)
                     /locus_tag="PLI11_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53193.1"
                     /translation="PLEFIAKRVCGDFQRGSWPETANEKRFAYFSPSAAVWAQEIIES
                     VFGINMNLIENKTFISPCMPESWDKAELKLPNIHLVYERKDGKFVVNAKIENDTSKVF
                     RILLPPYENIKAIVNGREAKVSTRRRCGFFEASFEMGNEREFLVEIEYRKIDYSINSP
                     KNIAVGDIFDISVSGLEILGIDDRCGIFSNVQFGRKGLKLTVKDTLLDNYEKFGYFGL
                     MNFARRVFVLKVKKDDIVFDLPVKLVLMKDVIFSATYEEGRVYVDLYNNMNKALSGNA
                     KLVLFCKAFDTECHVAARSVKQLVFDVKSVKGDMAPGMNNARIIVGDKVADIEVFADV
                     KADIRNVELDMEMIKPMQYWREIGLFQSHGHMMQGPDNFMKDLFETVDKVEVIEGVPF
                     KLSGGFIPLSFDKHRIVRIPLKGMKARKVYVLFSAFIDNHNVFGVPFTVELEIEKKDS
                     FIRPMYVKELAFPGEIDMGFGNNVIAGFATYVNGTKRGAVPGMPYTTEDGDYPNTRPH
                     IYPERDYWCKYKAVEVCNTVFNMIEIDTGKASDLKEIRFIANNADIGAGIYAINLF"
BASE COUNT          609 a          498 c          343 g          658 t
ORIGIN      
        1 ccgagagctt caacaagaac gaatatgttg aaaaggttat caatgatata atttataact
       61 tttcagaccc ggattttgat attaatgaag ccttggacaa gactcatcta aacaaggatt
      121 atttcaggcg tttgtttgta aagcatactg gaaagacacc actccagttc ctcacctcaa
      181 aacgcataac ctttgcaaaa cagcttttat ctactcaaaa gcaaacagac atgcaaatat
      241 gcgaaattgc atggagatgc ggtttttcca actgctacta tttttccagg gtattcaaga
      301 aggaaacagg aatgagccca aaggcttggg cagacagcta tgctaacgaa aaaaatattg
      361 atacagagca caatgagccc attccaacca ggtattttac gccacaatag tgtttaaaac
      421 agatttattg catatatgcc ggcaccgata tcagcattat tggctataaa tctaatctcc
      481 ttcaggtcgc ttgccttgcc tgtgtctatt tcaatcatat taaacacagt attacatacc
      541 tctacagcct tgtatttaca ccagtaatct ctttccggat agatatgagg tctggtattt
      601 ggatagtctc catcttcggt agtataaggc atgcctggta cggcacccct tttagtgcca
      661 tttacgtagg tggcaaatcc tgctattacg ttattgccga atcccatgtc tatctctcca
      721 ggaaacgcca gctcttttac atacataggt ctgataaaag aatccttctt ttcaatctcc
      781 agctctaccg taaagggtac tccaaacaca ttatggttgt ctatgaaagc actgaaaaga
      841 acatacacct ttcttgcctt catgccttta agaggtattc tcacaattct gtgtttgtca
      901 aaactaagag gaatgaatcc tccagaaagc ttgaagggta caccttcaat aacttccacc
      961 ttatccactg tttcaaacaa atctttcata aagttgtctg gtccctgcat catatgaccg
     1021 tgggattgga acaagccaat ttccctccag tattgcatgg gtttaatcat ttccatgtca
     1081 agttcaacat tccttatgtc tgccttgaca tcagcaaata cttcaatatc cgcaactttg
     1141 tctcccacaa ttattcttgc attgttcatg ccaggtgcca tatcaccttt gacgcttttc
     1201 acatcaaaca caagttgttt cacacttctt gccgccacat gacactctgt gtcaaaggcc
     1261 ttgcagaaca ggacaagctt tgcatttcca gacagagcct tgttcatgtt gttgtataaa
     1321 tctacataaa ccctgccctc ttcatatgtt gcactaaata ttacatcctt cataagtaca
     1381 agtttgacag gaagatcaaa tacaatatcg tcctttttaa ctttaaggac aaatactctt
     1441 ctcgcaaaat tcatgaggcc aaaatatcca aatttctcgt agttatccaa caaagtgtct
     1501 tttacagtga gtttcagacc tttcctgcca aactgtacat tgctgaaaat tccacacctg
     1561 tcatcaatgc ccagtatctc caatcctgaa acactaatat caaaaatatc tcccacagca
     1621 atatttttgg ggctgtttat gctgtagtct atttttctat actctatttc aaccagaaac
     1681 tccctctcat tccccatttc gaaggaagct tcgaagaatc cacaacgcct tcttgttgac
     1741 actttcgctt ccctgccatt aacaatggcc ttaatgtttt catatggagg cagcagtatc
     1801 ctgaatacct tgcttgtgtc attttcaatt ttggcattga caacaaattt gccatccttt
     1861 ctttcgtaaa ccaaatggat gtttggaagc ttgagttctg ccttgtccca actctctggc
     1921 atgcacggag atatgaatgt cttgttttct attaaattca tgttgattcc gaatacggac
     1981 tcaattatct cctgagccca cactgctgcc gaaggtgaaa agtaggcaaa tcttttttca
     2041 ttggcagtct caggccatga tcctctttgg aaatctccgc atactctttt tgcaataaac
     2101 tccagcgg
//
LOCUS       DAONVY010000421         2105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_35391_length_2105_cov_3.742927, whole genome shotgun sequence.
ACCESSION   DAONVY010000421 DAONVY010000000
VERSION     DAONVY010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35391_length_2105_cov_3.742927"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLI11_09760"
     CDS             complement(<1..823)
                     /locus_tag="PLI11_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPZ53194.1"
                     /translation="MKRRIVLLATLLVLVLTGCINKKEADRPLLITLHEFEADSNGVK
                     LEFHYAAKDDGQVLFYLEYEGEEHVQEGNGEGFYSFNIKEDNLESNTNFKLGVKDKKK
                     GRTMDEMEIQIKDYLVQLNERTVKCVVAQMTDEEKARLVIGYQAMPGDADFVDGVSGF
                     TYPLEKYGISSLVFSDGPAGVRNPKGSVSYPSGTNLAQTWDINLVKEIGKALGKDARN
                     LKVDILLGPGMNIHRNPLNGRNFEYFSEDPFLTGKIAAAYTKGVQSIGVGVSLKHF"
     gene            complement(825..1799)
                     /locus_tag="PLI11_09765"
     CDS             complement(825..1799)
                     /locus_tag="PLI11_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ53195.1"
                     /translation="MNKRIVLFVLMMVMLLCSCQNNKEKGDKTPDATLTPAVTETETP
                     SPTNTPKPRENLALGKKVEADVHELDYIPENAVDGDYSTRWSGLAPTDLPYHYLMIDL
                     GEVYALGKVCIDWEAIGHSYRISCAKKLDSDDDWEVVTTRLKPDDTYEEFNLGGIEAR
                     YIKLEFATNAFCSIYEIEIYEYDEDDENGDNNSRQNLALGKTATSEFVENGSEKFQAG
                     MAVDGDLLTRWSPGNGKANDLFTWHLDLGEVYDIKEIVIYFESCNTEYTISVSEDNQN
                     WTQVYEGEPLELEVSIKLNEKARYVLFSRPGGDWGSIYEFMVYDRLED"
BASE COUNT          547 a          508 c          374 g          676 t
ORIGIN      
        1 caaagtgctt tagcgacact ccaacaccta tcgactggac acccttggtg tacgctgctg
       61 caatttttcc tgttaaaaaa ggatcctcgg aaaagtattc gaagtttctg ccgttaagag
      121 gatttctgtg aatattcatt ccgggaccta gcagtatatc caccttcaga tttcttgcat
      181 ccttgcccag agcttttcca atttccttta ccagatttat atcccaggtc tgggcaagat
      241 tagtgcctga tgggtagcta acagagcctt tgggatttct tactcctgca ggaccgtcgg
      301 aaaacacaag agatgaaata ccgtatttct ctaaaggata tgtaaagccc gagaccccat
      361 caacgaagtc cgcatcgcct ggcattgcct gatagccaat aacaagcctt gctttctcct
      421 catctgtcat ttgggcaaca acacacttta cagttctttc attaagttgg acaaggtaat
      481 cctttatctg aatttccatt tcatccatgg ttctgccttt tttcttgtct ttaacgccca
      541 gcttgaagtt tgtattgctc tccagattat cctccttaat attaaaggag taaaaacctt
      601 ccccgtttcc ttcctgaaca tgttcttctc cttcatattc aagatagaac agtacttgcc
      661 catcgtcttt ggctgcatag tgaaattcaa gcttcacacc atttgaatca gcctcaaatt
      721 catgcagggt tataagcagc ggcctgtcag cttccttttt atttatacat ccggtcaaaa
      781 caagtaccag caatgttgcc agcaaaacta tccttctttt cacgtcaatc ctccaatctg
      841 tcatatacca tgaactcata tatgctgccc cagtctccac ccgggcgtga gaaaagaacg
      901 tatcttgcct tttcgtttaa tttaatcgac acttccagtt caagtggttc tccctcataa
      961 acctgggtcc agttttggtt gtcctctgac accgatatgg tatattcagt attgcaggat
     1021 tcaaagtata ttacaatttc ctttatgtcg taaacctctc ccagatcaag atgccatgtg
     1081 aacaaatcat ttgcttttcc gtttccagga ctccatcttg taagcaagtc gccatcgaca
     1141 gccatgccgg cttgaaattt ttcgcttcca ttctctacaa actcggaagt tgcagtcttg
     1201 ccgagagcta ggttttgcct gctgttatta tcaccgtttt cgtcatcctc gtcatactca
     1261 tatatttcaa tttcatatat gctgcagaat gcgttagtag caaactcaag ctttatgtac
     1321 ctggcttcaa ttccaccaag attgaactcc tcgtacgtat catcgggctt gagtcttgtt
     1381 gttacgacct cccagtcatc atcggaatca agcttctttg cacagcttat acggtaagag
     1441 tgccctatgg cctcccaatc gatgcaaacc tttcccaatg catatacttc tcccagatct
     1501 atcattaaat aatgataggg aaggtcagtt ggtgcaagac cgctccatct ggtcgaataa
     1561 tccccatcca cagcattttc cggaatgtaa tcaagttcat gtacatcggc ttcaactttt
     1621 ttgcccagcg caagattctc cctgggtttt ggagtatttg taggtgatgg cgtttccgtc
     1681 tctgtcacag caggagttaa agtagcatca ggagtcttgt cccctttttc cttgttattc
     1741 tgacatgaac acaacagcat taccatcatc aatacaaaca atacaatgcg tttattcatt
     1801 ttaaaacctc cgatcaaata actgctctga gtaaattata taccatcctg tactatgtac
     1861 aattcccttt ttgcagaaaa cttctcatat cttgctcaaa atgcttgccc ggtacaataa
     1921 attgcaggta taataggtgt gggaagtgga taacatgtct ggttttcttt atgagcgtgt
     1981 taattatgac aaaaacataa aaatgtttct tgctccgctt actgctgtgt ttgtagacat
     2041 gcatgttcat gacgaactgg aggttatttg cgtattaaat ggagacatgt atgttctggt
     2101 caaca
//
LOCUS       DAONVY010000422         2099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_35525_length_2099_cov_3.476517, whole genome shotgun sequence.
ACCESSION   DAONVY010000422 DAONVY010000000
VERSION     DAONVY010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35525_length_2099_cov_3.476517"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..1939)
                     /locus_tag="PLI11_09770"
     CDS             complement(398..1939)
                     /locus_tag="PLI11_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53196.1"
                     /translation="MTEFSFLRNMRIFIIVGVLMLSLGACQSSQDIEFEVETDDVSTF
                     RGSDGKIVVKTKGISNSFEYSIDNGETWSNSNTFKSLSAGLYRIKIRNKGLPRKTSQV
                     KYVMLSQPEAKRPEVYVGVADQRLEQLNNPDKWKFVRENADGFYINFIMLDSIYSQKD
                     LNKFAELFTNKNAFIESDMNSTMKKEQGYIDRLHKAGFRIPYTSLNYGWEKSRHDNLK
                     TYNLLAGQEPRLCLVQQGPWCIGGDITKDEGILPEPYTNANYRSWIEQADGASTDGPM
                     GFWYSDENSMKSGSYSMVKFARSLGKKSLVMICPYHADVKGYNPNMYLEVGMQCVREH
                     EDNDAEPDIWSVFEYATSIAAVPEEKDGKPFNSTTGMAYYLINHILGVPDTLSFTVGN
                     TDKDIQLDASVNPGTMLSYEIEVKNTSDWCDYAAGLKANIEDLTEAWDVRFMQGDKDV
                     TNLVLSGSFQFYKEHRLNPQSTQKLTLVFTRTNTSKSTNFTMELFLSRHLGVEGVQSI
                     KIIAK"
BASE COUNT          556 a          448 c          384 g          711 t
ORIGIN      
        1 agccttgtct tcacacaatc caatgtatat gtcgtcccaa ttttaccttc ttttgctttt
       61 atgtataacg caccaagagc gattcttgcc gataaagctt cgttgcctct tttcgacttg
      121 aattgctgac tgtattttct tctagtttat cccatggaat catttgagcc ctttgaccca
      181 attattattc gggtcaagat gtgcgctaca catttattag tgaaaggtaa gaaatatttg
      241 cactaaaaga tgaattctgt atgcaaattg tacttgtaca atatggtata ttaagcatac
      301 agaattcatg tttgtagtgg ttatagtctt atatagcggt gtaaattccg gaacgaatta
      361 atcacttttt ggtagcaaaa tattacaacc cgccttctca tttggcaatt atctttatag
      421 actgaactcc ttccactccc agatgtctag aaaggaagag ttccattgta aaattggtac
      481 tctttgatgt atttgttctt gtaaagacga gtgtgagctt ctgggtgctc tgaggattca
      541 gtctgtgttc cttgtagaac tggaatgaac cgcttaacac aagatttgtt acatctttgt
      601 caccttgcat aaaccttaca tcccaggctt ctgtaagatc ctcaatgttt gctttcaagc
      661 cggctgcgta atcgcaccag tcgctggtgt tttttacctc aatttcatat gaaagcattg
      721 tgccgggatt tacgcttgca tcaagctgaa tatccttgtc ggtattaccc acggtaaagc
      781 ttaaagtatc aggcacgccc agtatgtggt taatcaggta gtaggccatg ccggtagtgg
      841 agttaaaagg ttttccgtcc ttttcttcag gcaccgccgc aatactggtc gcatactcaa
      901 acacagacca gatatcgggt tccgcatcgt tgtcttcatg ttctcttaca cattgcattc
      961 ctacctcgag atacatgttg ggattgtatc cttttacatc agcatggtaa gggcatatca
     1021 ttacaagtga cttcttgcct aaagaacggg caaacttgac cattgaataa ctgccgcttt
     1081 tcatactgtt ctcgtcagag taccaaaagc ccataggacc gtccgtggat gccccatctg
     1141 cctgttctat ccaagacctg tagttggcat tcgtatatgg ttcgggtagt atgccctcat
     1201 cttttgttat gtcgccaccg atacaccagg gtccctgctg aacaagacac agtcttggtt
     1261 cctgacctgc cagcaggtta tatgttttta aattgtcatg tcttgacttt tcccagccgt
     1321 agttaaggct tgtgtatggg attctgaaac cagccttgtg cagcctgtca atatatccct
     1381 gttccttttt cattgtggaa ttcatgtcgg attcaataaa ggcatttttg ttggtaaaca
     1441 gttctgcaaa cttgttcaga tctttttgag aatatattga atcgagcata atgaagttta
     1501 tgtagaagcc gtctgcgttt tcccttacaa acttccattt gtcaggatta ttcagctgtt
     1561 ccaggcgctg atctgcaaca cctacataaa cctcaggtct ttttgcttct ggttgagaca
     1621 acattacata ttttacttgt gatgtttttc ttggcaatcc tttattccta atctttatcc
     1681 tatacaatcc agcggaaagt gatttaaaag tattagagtt cgaccaggtt tctccgttgt
     1741 caatactgta ttcaaaacta ttggatattc ccttcgtttt gacaactatt ttcccgtcag
     1801 aaccacgaaa agtagaaaca tcatcagttt ccacttcaaa ttcaatatcc tgcgatgatt
     1861 gacaagctcc taatgaaagc attaaaacac ctacaattat aaatattctc atatttctaa
     1921 gaaatgaaaa ttccgtcata aaaatcacct atccttcgaa aatcattcca catctatact
     1981 cataagttca ataaactttg ttgcagcttg tgaaaaagga attcctctca attttactag
     2041 tcctatggac ctttttggta ttgggggagt taatggtatc tcaaaaagat tctcgttgt
//
LOCUS       DAONVY010000423         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_35657_length_2092_cov_3.289151, whole genome shotgun sequence.
ACCESSION   DAONVY010000423 DAONVY010000000
VERSION     DAONVY010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35657_length_2092_cov_3.289151"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..718)
                     /gene="hpt"
                     /locus_tag="PLI11_09775"
     CDS             complement(146..718)
                     /gene="hpt"
                     /locus_tag="PLI11_09775"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPZ53197.1"
                     /translation="MSNKIYEYDVPLVDYTDGIVATEEEIADMCRRLGERISNDYKGE
                     PLVLICVLKGGFMFMSDLAKNISIPVEMDFIAVSSYGASTKSSGVVRLVKDTDTPVTG
                     KHVLIVEDIIDTGLTLSYLKNLFSTRNPLSVKVCTAFDKPDRRKVEVDVEYSGIVIPD
                     EYVVGYGLDYNGLFRNLPDLRILKKSVYVK"
     gene            complement(708..>2092)
                     /gene="tilS"
                     /locus_tag="PLI11_09780"
     CDS             complement(708..>2092)
                     /gene="tilS"
                     /locus_tag="PLI11_09780"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPZ53198.1"
                     /translation="DRFEETIAKHNMIDKGVIACVSGGADSICMLYLLYDYCSKHDVD
                     LVCAHFNHCIRGEESDSDMEFVKDVCKKLGIKLYFESGDVPSLAKKLSKGIEETARIA
                     RYEFFEKLAKETGYRICVAHNRDDRIETVIHNIFRGTSIDGLKGIPYTRGNIIRPMLD
                     IGRKEIEEFLSQNNIEFKVDSTNADVTYIRNRIRHKIIPFLENEYGSIKESIIRLSDL
                     SIEDSDFLNSVASEKALECIIPDSYVKIDISRYSKNHVAIRGRILKIALGMLLDEEGK
                     RLFEAGKGIEMSIIKRVDAFALYGHSGKSVDVSKGVVARKEYDTLVFVFDEYNHLSKS
                     DCCLTFEIVPYSSFDKSLLNDLGCACFDYDKLLEEVNIEELKVRTRKEGDVFTPFGFS
                     GTKKLKKFLIDEKIPVSERNRIMLLAHGKCILWIPNIRRSNMAPIDKNTQNVIIFRLH
                     YKKWRKTNEQ"
BASE COUNT          607 a          486 c          302 g          697 t
ORIGIN      
        1 ataatatttt cgagcattat gacaacaaaa ataatcagtg ctccaattat aatatatacc
       61 gatatatttt taaacgcctt cacaatctac ctccaatgtg tttattaaaa atcgtaccat
      121 aataattggg ttttatcaac tttttctact tgacatacac ggacttcttg aggatcctca
      181 agtcaggcag attcctaaac aatccattgt aatcaagacc gtatcctacc acatattcat
      241 caggtattac aataccgcta tactccacat caacctcaac ttttcttctg tcaggtttgt
      301 caaaagctgt acataccttg acacttaatg gatttctggt tgaaaacaga ttcttcaggt
      361 atgacaaagt cagccctgta tctattatat cctctacgat aagaacatgt ttgccagtta
      421 caggtgtatc ggtgtctttt actagacgga caactcctga tgattttgtt gatgctccat
      481 aacttgaaac ggcaataaaa tccatttcaa caggtatgga tatattcttg gcaaggtcgg
      541 acatgaacat aaaaccgcct ttaagaacac atatcaaaac caatggttct cctttgtaat
      601 cattgcttat tctttctcca agcctcctac acatgtcagc tatttcttct tccgtagcca
      661 ctattccatc tgtgtaatca accaatggta catcgtattc atatatttta ttgctcatta
      721 gtcttcctcc attttttgta gtgcaacctg aatattataa cattttgtgt atttttatca
      781 ataggtgcca tattgcttct ccttatattc ggaatccata gtatgcattt tccatgtgca
      841 agaagcatta ttctgtttct ttccgacaca ggtatttttt catcaatcag gaactttttc
      901 aatttctttg tcccactgaa accaaaaggt gtgaatacat cgccttcctt ccttgtccta
      961 accttaagct cctctatatt gacttcctcc aacaatttgt cataatcaaa acatgcacat
     1021 cccaaatcat tcaaaagcga tttgtcaaag gaactgtacg gaacaatttc aaaggtcaaa
     1081 caacagtcgg attttgaaag gtggttatac tcgtcaaaca caaacaccaa agtatcgtat
     1141 tccttcctgg caacaacacc ctttgaaaca tccacggatt tgcctgagtg accataaaga
     1201 gcaaacgcgt ccacccgctt tataatcgac atctcaatac cctttccggc ttcaaacaga
     1261 cgtttgccct cctcatccaa aagcatgcct aaagcaatct tcagtattct tccccttatt
     1321 gccacatgat tctttgaata ccttgatata tctattttta catatgaatc tggaataatg
     1381 cattccagag ccttctcact tgcaacagaa tttagaaaat cactgtcctc gatgcttaaa
     1441 tcagaaagtc ggattatgga ttcttttatg ctgccatatt cattttccag gaaaggaata
     1501 attttatgcc ttatcctgtt tcttatgtat gtcacatcgg catttgtact gtctactttg
     1561 aactcgatat tgttttgact taagaattct tcaatttcct tacggccaat atcaagcatc
     1621 ggcctgatta tattgcccct ggtataaggg atgcctttca agccatcaat ggatgtacct
     1681 ctaaaaatat tgtgaatgac agtctcaatc ctgtcatccc tgttgtgggc aacacaaatt
     1741 ctataccctg tttcttttgc aagcttttca aaaaattcat accttgcaat ccttgctgtt
     1801 tcctctatac ccttgctaag ctttttggca agagaaggca catcccccga ctcaaaataa
     1861 agctttatgc caagtttttt gcaaacatct tttacaaact ccatgtcgga gtctgattcc
     1921 tcgcctctta tgcagtgatt gaagtgggca cataccaaat ccacatcatg tttcgagcaa
     1981 tagtcataaa gaagatacag catgcatatt gagtcagctc cacccgatac gcatgctatc
     2041 acacctttgt caatcatgtt gtgtttggct attgtttctt caaacctatc at
//
LOCUS       DAONVY010000424         2075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36029_length_2075_cov_3.592079, whole genome shotgun sequence.
ACCESSION   DAONVY010000424 DAONVY010000000
VERSION     DAONVY010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36029_length_2075_cov_3.592079"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PLI11_09785"
     CDS             <1..1125
                     /locus_tag="PLI11_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPZ53199.1"
                     /translation="VPFRHVLIHGLIRDEQGRKMSKSLGNGIDPLEVIDEFGTDALRF
                     TLISGNSPGNDQRYLPKKTEAARNFANKIWNATRFVLLNFDEDVDFSKVDRSKFTVYD
                     KWILSASNNLVKEVTENLNKYELGIALGKVYDFIWDVYCDWYIEIVKPRLFDKENETR
                     LEAQYVLNEVLVKCMKLLHPFMPFITEEIYQSLIHESESIMISDWPEFDESLVFAEDE
                     EKVNIIIKAIRSIRNIRTEMNIPPSRKAGIIFVTDENKFELLQDSESLFNRLASANGI
                     KTTSDRSTIPENSATAVVPGIEIFIPLEELIDYEKEIERLENELKHLQDELDRVNKKL
                     ANENFVKKAPENIVEEERKKKEKYTEMYNNVLERIKILKK"
     gene            1137..>2075
                     /locus_tag="PLI11_09790"
     CDS             1137..>2075
                     /locus_tag="PLI11_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53200.1"
                     /translation="MKRNKVMSFIRLGILVSFAVVYAVLSYYTKPRIIRYDVYEKIDT
                     SKYGSEYNIARMFENCLVMNVDTSNVYYNGEYLSYSDIKNLLVFEDGRFFCNSAFINQ
                     LLDKDYSGDRVDLEELGYEVLNYNNRMCIVDMGEKDISLFDNLYTAEALYLRLSGKEQ
                     EDIENAFVDLPYLISNGRNNAVFYSEPSLNLGIQTEIYWHQINRDDSRPEFVVGEGEY
                     DDNSTLVRVFNKMQTCTQQFLAYNSYVKGGVQVKALKSKEDVLIATAPFKSWPLSARR
                     IRIFNTSGSLCMEIIPNLTAPYVIETGYFTGNDSEQL"
BASE COUNT          676 a          344 c          469 g          586 t
ORIGIN      
        1 gttccattca gacatgtcct tattcacggt cttataagag acgaacaagg acgcaagatg
       61 tccaaatcct tgggcaacgg tattgacccg ctcgaggtta tagatgagtt tggtacggat
      121 gctttgcgtt ttaccctcat ttctggaaac tcaccaggaa acgaccagag atatctgcca
      181 aagaaaactg aggcagcaag aaactttgca aacaaaatat ggaatgcaac caggtttgtt
      241 cttctcaact ttgacgagga tgtggatttt tccaaggttg acagaagtaa attcacagtt
      301 tatgacaagt ggattctgtc cgcttcaaac aatcttgtaa aagaggttac tgagaatctc
      361 aacaaatatg agcttggtat tgcccttggc aaggtatatg actttatatg ggatgtttac
      421 tgtgactggt atattgagat tgtcaagccc agactgtttg acaaggaaaa cgaaacaagg
      481 cttgaggccc agtatgtttt gaatgaggtg cttgtcaaat gcatgaagct tttgcatcct
      541 ttcatgccct tcataactga agagatttat cagagtctta tacatgaaag tgaaagcatt
      601 atgatttctg attggcctga gtttgatgag agtcttgttt ttgcagagga tgaagagaag
      661 gtaaatatta ttatcaaggc aatcagaagc ataaggaata ttcgaactga gatgaatatt
      721 ccgccttcaa gaaaagctgg tatcatattc gtaactgatg aaaacaagtt tgagcttttg
      781 caggattcag agtcgctttt caacagactt gcttcagcaa atggaatcaa gactacaagc
      841 gacaggagta ctattcctga aaacagtgca actgctgtgg ttccaggtat tgaaattttc
      901 attcctcttg aggaacttat agattacgag aaggaaatcg agcgtttgga aaacgagctt
      961 aagcacttgc aggatgaact tgacagggtt aacaaaaagc ttgctaatga aaactttgta
     1021 aaaaaagctc ctgagaatat cgtagaagaa gagaggaaga agaaagagaa atacacagag
     1081 atgtataaca acgtcttgga gagaataaaa atactaaaaa aataggagag tagagtatga
     1141 agcgtaacaa ggtcatgtca tttatcaggc tcggcattct cgtttcattt gctgttgtat
     1201 atgcggtttt gtcctattat acaaaaccaa ggattatccg ctatgatgta tacgaaaaaa
     1261 tagatacatc aaaatacgga tccgaataca acattgcaag gatgttcgag aattgcctgg
     1321 ttatgaatgt cgacacttca aacgtgtact acaatggtga atacttgtca tactctgata
     1381 ttaaaaacct actggtcttt gaagacggta ggtttttctg caattccgct tttataaatc
     1441 agctgcttga taaggattat tcaggcgaca gagttgactt ggaggaattg gggtatgaag
     1501 tattaaacta taacaacagg atgtgcattg tggacatggg agagaaagat atttctctat
     1561 tcgataactt gtacaccgca gaagctttgt atttaagatt gagtggcaag gaacaggagg
     1621 atattgagaa tgcgtttgtt gacctgcctt atctcatatc taatggcaga aacaatgcag
     1681 tattctattc tgaaccaagt ctaaatctag gcattcagac agaaatatat tggcatcaga
     1741 ttaacaggga tgattcaaga cctgaatttg ttgttggaga aggtgagtat gacgacaact
     1801 caacgctcgt tagggtgttc aacaaaatgc agacttgcac acaacaattc ctggcttaca
     1861 attcttatgt aaagggtgga gtgcaggtta aagctctaaa aagcaaggaa gatgtgctga
     1921 ttgccaccgc tcccttcaag tcatggcctt tgtcagcaag aaggataaga atttttaaca
     1981 catctggaag tttgtgtatg gagattatcc cgaatttgac tgctccatat gtaatagaga
     2041 cgggttattt tactggaaac gacagcgagc agctg
//
LOCUS       DAONVY010000425         2074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36042_length_2074_cov_4.504210, whole genome shotgun sequence.
ACCESSION   DAONVY010000425 DAONVY010000000
VERSION     DAONVY010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36042_length_2074_cov_4.504210"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLI11_09795"
     CDS             <1..423
                     /locus_tag="PLI11_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53201.1"
                     /translation="SRQLWHIIVVCKSEVFAYRWEAEHSIVLGNANNRGSHVGGIDKA
                     GDGIAFKIHVSENNMYCFRIYYAAFLGDASHDLFVNGKKAAVVNYKKTDLGWGVFDDE
                     TYVEVFVPLTEGTHAISIQKTNLSTGFAEIDAVYVPWQ"
     gene            514..897
                     /locus_tag="PLI11_09800"
     CDS             514..897
                     /locus_tag="PLI11_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPZ53202.1"
                     /translation="MKIRHYSKTDEEALFDLLMDEGDEWIDYHGKNGRSKYIKALESS
                     ITYIACDENVVCGYARCREDDGFGVYIYDLLVRKTYRGRKIGKCIMERVCQDFPDQPV
                     YVMSDVDSYYEKLGYIREGSIFRVK"
     gene            1223..2050
                     /gene="rpsB"
                     /locus_tag="PLI11_09805"
     CDS             1223..2050
                     /gene="rpsB"
                     /locus_tag="PLI11_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPZ53203.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTVKMIDEAYYFLVDVARNGGTVLFVGTKKQARDSIYEEATRCDMYYVNNRWLGGTLT
                     NFKTIRKRIEKLHQLNEMEEKGLFERLPKKEVVKLKQERDKLEKNLGGIKGLDKLPSA
                     IFVVDPRVEKNAVLEARKLGIPVVAIVDTNCDPDEVDYVIPGNDDAIRAVKLIASTMA
                     DAVLEGRQGVSFAKDAEVEEDDSLEHTEVEEVADVEEPQDEIEVADEAEEDTAKSEE"
BASE COUNT          702 a          287 c          508 g          577 t
ORIGIN      
        1 agccgacagc tgtggcacat tattgttgtt tgtaaaagtg aagttttcgc atacagatgg
       61 gaggctgaac attcaattgt attgggaaac gccaataata gaggaagcca tgtaggtgga
      121 attgataaag ctggagatgg aatagctttt aagattcatg tttctgagaa taatatgtat
      181 tgtttcagaa tatactatgc agcattttta ggagacgcaa gtcatgattt gtttgttaat
      241 ggaaagaagg cagcagttgt caattacaag aagacggatt tgggatgggg agtttttgat
      301 gatgagactt atgttgaagt ttttgttcca ctaacagaag gtacccatgc aatttcaata
      361 caaaaaacca acttgtccac ggggtttgct gaaatagatg cggtatatgt gccttggcaa
      421 tgatttgacc aaaacaatat taacacaatg aaaaaaatag tataatatgg gtacatagtg
      481 ttttaatgtt gcacatctgg aggagttttc gccatgaaaa tcaggcacta cagtaaaacc
      541 gatgaagaag cgctttttga cttgctcatg gatgagggtg acgaatggat agattatcac
      601 ggcaaaaatg gccgcagcaa atacatcaag gcgcttgaat caagcatcac atatattgct
      661 tgtgatgaga atgttgtgtg cggttatgca cgctgtcgtg aagatgatgg atttggcgtt
      721 tatatatatg atttgctggt cagaaaaacg tatcggggaa gaaagattgg gaaatgcata
      781 atggagcggg tatgccaaga ctttcctgat cagccggtct atgttatgag cgatgtcgat
      841 tcatattatg agaaactggg ttatattaga gagggttcaa tatttagggt taagtaaaag
      901 agcgattatt aggggttttg caatattaaa tatatttttg gaagtagtaa aaggcaaaaa
      961 acagaaagca ttacaatttg agttttgata ttttgagtat aatgctaact gtttatcttc
     1021 ttattgatat atatttgcaa tgcattatcc gttgtatagg gtaaaaatga tgttgcaatg
     1081 ctaaacgata tctggtataa tgcgatagtt ataaaaaaat aatcacggtt gtctgattcg
     1141 ctaaggttgg atcctggaca catggatctc ttgagcgagt tgaagatgcc ggaagttaaa
     1201 acaaaaggag gataaattat ttatgtcagt tatttcaatg aaacaattac ttgaagcggg
     1261 tgttcatttt ggtcatcaga ccaggagatg gaaccccaaa atggctgagt acatttttac
     1321 agaaagaaac ggaatctata tcatagattt gcagaagact gtgaagatga ttgatgaagc
     1381 ctattatttc cttgttgatg tagccagaaa tggcggaaca gttcttttcg taggtaccaa
     1441 gaagcaggca agagattcca tatatgagga agctacaaga tgcgacatgt actatgtaaa
     1501 caacagatgg cttggtggta cacttacaaa cttcaaaaca atcagaaaaa gaattgaaaa
     1561 attgcatcag cttaacgaaa tggaagagaa ggggcttttt gaacgtcttc caaagaaaga
     1621 agttgtcaaa ctgaaacaag agagagacaa gctcgagaag aacctaggtg gtatcaaggg
     1681 actggacaaa ctcccatcag ctatttttgt agttgaccca agagttgaga aaaatgcagt
     1741 attggaagct agaaaactgg gtataccagt agtggcaatc gttgacacaa actgcgatcc
     1801 tgacgaagtg gattatgtaa tcccaggcaa tgacgatgct attagagcgg taaaattgat
     1861 tgcttcaaca atggccgatg ctgttttgga aggaagacaa ggggtatctt ttgctaaaga
     1921 cgctgaagtt gaagaagacg actctttgga acatactgaa gttgaagagg ttgcagatgt
     1981 tgaagaacct caggatgaga tagaagttgc agacgaagct gaagaagaca cagctaagtc
     2041 ggaagaataa tactaagggg gtataggaat gaca
//
LOCUS       DAONVY010000426         2070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36143_length_2070_cov_3.060546, whole genome shotgun sequence.
ACCESSION   DAONVY010000426 DAONVY010000000
VERSION     DAONVY010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36143_length_2070_cov_3.060546"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1353
                     /locus_tag="PLI11_09810"
     CDS             76..1353
                     /locus_tag="PLI11_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPZ53204.1"
                     /translation="MKKLNYGRTILIGLAFFSICAFWQVFDNIIPLMLKESFGLRDAA
                     TNSIMALDNILALFLLPFFGSLSDRTNTRYGKRTPYIILGTVFAVILMMFLPVASNKN
                     SLIMFFIALGLVLLAMGSYRSPAVALMPDLTPKRHRSKANAIINLMGALGGIYTLVCI
                     RLLVPDKENPSYTLVFLAVAILMVVSVTILKLTINEKKLSKQIKAVDSDFEDEPVTGE
                     KAAPLPKEVRKSLILILSSIFLWFCAYNAVITAYSRYAKEVWGITGGAFADSLMIATG
                     AAVVSYIPIGFIATRFGRKRTILAGICMMFVAFSTGFFITKVSPVIYIVFVLTGIGWA
                     AINVNSYPMVVEMSKGSNIGKYTGYYYTFSMAAQIFTPILSGFFLEVSYRTLFPYAAV
                     FMVLAFITFTFVKHGDSKPVRKKDLIENFDIED"
     gene            1400..1475
                     /locus_tag="PLI11_09815"
     tRNA            1400..1475
                     /locus_tag="PLI11_09815"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1433..1435,aa:Ala,seq:ggc)
BASE COUNT          615 a          410 c          402 g          643 t
ORIGIN      
        1 ttgtaccaca gaggattgaa gtcctcaatt ttcatttaag tttacagagc gatattaaac
       61 atccggaggt aaatcatgaa aaagcttaac tatggacgga cgattttaat agggcttgca
      121 tttttctcca tatgtgcgtt ctggcaggtt ttcgacaaca tcattccact catgctaaag
      181 gaatcattcg gcctgcgcga tgctgctaca aattcaatca tggcattgga caacatactt
      241 gctctgttcc tgttgccctt ttttggttcc ctctctgaca gaaccaacac ccgctatggc
      301 aaaagaacac cttacataat attgggaaca gtgtttgcag taattctcat gatgtttctg
      361 cctgttgcca gcaacaaaaa cagcctcatt atgttcttca ttgctctagg tcttgtattg
      421 ttggcgatgg gctcctacag gtcccctgct gttgccctga tgccagatct tacaccaaaa
      481 aggcaccgca gcaaggccaa cgcaataatc aacctaatgg gtgcactggg cggcatatac
      541 actcttgtgt gtatacgcct tcttgttcca gataaggaaa atcccagcta tacattggtg
      601 tttctggcag ttgccatact gatggttgtt tctgttacaa ttctgaaact cacaattaat
      661 gagaaaaagc tttcaaagca gataaaagct gtagattcgg atttcgagga cgaaccagta
      721 acaggcgaaa aagcagctcc tcttcctaaa gaagtacgaa aaagtctcat actcatcctt
      781 tcttcaatat tcctttggtt ctgtgcatat aatgcggtta taacagcata ttcaagatac
      841 gccaaagaag tctggggcat aaccggtgga gcttttgcag actcattgat gatagcaaca
      901 ggagctgcgg ttgtaagcta cattccaatt ggatttattg caacccgctt tggaagaaaa
      961 cgcactatcc ttgcaggaat atgcatgatg tttgtcgctt tcagcacagg attcttcata
     1021 accaaagtat ccccggtaat atacattgtg tttgtgctga caggtattgg ctgggctgcc
     1081 atcaatgtta actcctatcc gatggttgtt gagatgagca agggctcaaa cattggcaaa
     1141 tacacaggat attactatac cttctcaatg gctgcacaga ttttcacccc catactttca
     1201 ggctttttcc tggaggtaag ttacaggacc ctgttcccct atgcagctgt cttcatggtg
     1261 ctggcattta ttacattcac cttcgtaaaa cacggagatt caaaacctgt gagaaaaaag
     1321 gatttgatag aaaacttcga tatcgaagac taaatatgtt tacaattcgt ctcaaatgtg
     1381 ttatactcgt ttgagacagg gggcattagc gcagttggga gcgcaccaca ctggcagtgt
     1441 gggggtcgtg ggttcgaatc ccatatgctc caccattcat tgcaaaaagc aaccggattg
     1501 gttcgctttt tgcaataata tttgttcctt gtaaaatgaa tagaatgtgt cccacagatt
     1561 tatgagttgg tgaggatatg gtttttcgtg ttaaaggact tttttaaaag aaaacattga
     1621 atgaagacgg taaatttatt tcactaataa ggcacaattt cctgacaaaa gatactcagc
     1681 tttatattcc tttaactgac caaaagtata cttcacattt tgatttattt gttactgatt
     1741 caggcgagaa ggataaaaaa catatttttc accaatacag tataaagcaa gatagcaaat
     1801 caataacaac cgcaaccgtg agatttagtg tatttgaaaa tataacttct tgtgcgtttg
     1861 actataataa caaacattat gttattaaat cttaaaataa atttgctaat gaatttaacg
     1921 tatttaataa tgataatatt gttgcaacag tttgtgttcg tgcattttcc agagatgtga
     1981 agtctgacga aattacagat cctcttttga tttgtatttt ctatatattt tcagcataca
     2041 ttaattctga acacaatatt tcatacagtg
//
LOCUS       DAONVY010000427         2068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36184_length_2068_cov_3.278192, whole genome shotgun sequence.
ACCESSION   DAONVY010000427 DAONVY010000000
VERSION     DAONVY010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36184_length_2068_cov_3.278192"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="dnaG"
                     /locus_tag="PLI11_09820"
     CDS             complement(<1..1055)
                     /gene="dnaG"
                     /locus_tag="PLI11_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPZ53205.1"
                     /translation="MIPESQVEDIISRNDLVDVINEYTHLARRGSGYMGLCPFHREKT
                     PSFSVHEGKQIFKCFGCGKGGNVVHFIMLAEGLDYRSALEFLANRVGIEIETTRSRGS
                     STTKLRQDIMSVNKEAARFFFRNLIDNKIAQKYCVDRGIDVKTIKKFGIGFAPDAWSA
                     LREHLKAQGVSDELLLKAGLILQGKENSTYDRFRNRLMFPIFDVLGNVIAFGGRVLDD
                     SMPKYLNSPETVLYTKGNHLYALNFARKSESKRVFIVEGYMDCIALNQRGITYSVASL
                     GTALTDNQAKLLKKYFDEVIISYDADTAGQNATIRGMEILQKHGFRVKVLELPKGDDP
                     DDYIRKYGRDKFFELADG"
     gene            1248..1529
                     /locus_tag="PLI11_09825"
     CDS             1248..1529
                     /locus_tag="PLI11_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53206.1"
                     /translation="MINKFKRLDKSFIQLFKQLSQISASALSAGIWTSILCSIIGICI
                     MLYLYIGGNATPTIFNTAKYIIKSGTGMLLLAAIVAVIVEFVKMGEARE"
     gene            complement(1532..>2068)
                     /locus_tag="PLI11_09830"
     CDS             complement(1532..>2068)
                     /locus_tag="PLI11_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HPZ53207.1"
                     /translation="EMGFKQISVEPVVAPDGSGYELKEEHLPILFKEYEKLAKYYAEK
                     WQTDDWFNFFHFMIDLTQGPCVAKRVRGCGAGSEYVAVTPEGDIYPCHQFVGQTEFII
                     GNVYGNELDYELMDTFKEKNVFTKKSCMDCWARYYCSGGCHANSWNLNKDLDIPYELG
                     CELQKKRIECAIWAKAME"
BASE COUNT          594 a          512 c          325 g          637 t
ORIGIN      
        1 ccatctgcca gttcaaagaa cttgtccctt ccatactttc taatatagtc gtccgggtcg
       61 tctccttttg gcaattccag aacttttacc ctgaaaccgt gcttttgcag tatttccata
      121 cctcttatgg ttgcgttttg acctgcagta tccgcatcat aacttattat cacttcatca
      181 aagtactttt tcaatagttt tgcctgatta tcggtcagag ccgtaccaag cgaagcaact
      241 gaatatgtta ttccccgttg attaagagct atgcaatcca tatatccttc aacaatgaaa
      301 acccttttac tttcactttt tcttgcaaaa ttcaaggcat acaaatggtt gcctttggta
      361 taaagtacgg tttcgggaga attcaaatac tttggcattg aatcatccag aactcgtcca
      421 ccaaaagcaa tcacgttacc cagaacatcg aaaataggaa acataagacg gtttctgaat
      481 ctgtcatatg tactgttttc cttaccctgc aagattaacc ctgctttcag tagaagctca
      541 tcgcttactc cctgtgcctt taaatgttcc ctcaaagcac tccaggcatc aggtgcaaaa
      601 ccaatgccaa acttctttat tgtctttaca tcaattcctc tatcaacgca atatttctgc
      661 gcaattttat tgtcaatcag gtttctgaag aaaaatcttg cagcctcctt gtttacagac
      721 ataatgtcct gcctaagctt cgttgtactg cttccacggc ttctagtggt ctcaatttca
      781 atacccaccc tgtttgccag aaactccaat gctgatctgt aatccaatcc ctcagccagc
      841 attatgaaat gtacaacatt tcctcctttt ccgcaaccaa aacatttgaa aatctgtttt
      901 ccttcatgca cggaaaagga cggtgtcttt tccctatgaa aagggcaaag ccccatgtat
      961 ccgctacccc tgcgagcaag gtgcgtatac tcgttaatca cgtcaacaag gtcatttctg
     1021 ctgattatat cttcaacttg tgattcaggt atcaactata tgtacacccc tgtccattac
     1081 ggctttccgc cttggctata agccatggca ataacagcct gcaattatat aatacgacat
     1141 cggcatcaaa aaaccttttt ttattactta ccaatattta atcattgagt tattgcttgt
     1201 aattttgcaa aatgtatcta caagaataaa ttttgcaggt gattaatatg ataaataaat
     1261 tcaaaaggct tgacaaaagc tttatacaat tattcaaaca gctatcacag atttccgcca
     1321 gcgcattaag cgcaggaatc tggacaagca ttctatgttc catcattggc atatgtatta
     1381 tgctttactt gtacatagga ggaaatgcaa cccccacaat attcaatacc gccaagtaca
     1441 taataaaaag tggaacaggc atgcttttgc tcgctgcaat tgtagcagta atagttgagt
     1501 ttgtcaaaat gggagaagcc agagagtaac actactccat ggcttttgcc catatggcgc
     1561 attctattct tttcttctgc aattcacagc ctagttcgta aggtatatcc aaatccttgt
     1621 tcaaattcca actattcgca tgacaaccac cgctgcagta atatctcgcc cagcagtcca
     1681 tgcacgactt cttcgtaaac acattcttct ctttgaaagt atccatcagc tcataatcaa
     1741 gctcgttgcc atatacatta cctataataa attcagtctg tccaacaaac tgatggcaag
     1801 gatatatatc cccttccggt gttacggcaa catactcgct gcctgctcca caacccctta
     1861 ccctttttgc aacgcaaggt ccttgtgtca agtcaatcat aaaatgaaaa aagttgaacc
     1921 agtcgtccgt ctgccacttt tccgcatagt attttgcaag cttctcatac tctttgaaca
     1981 agattggcaa atgctcctcc tttagctcgt aacctgaacc atcaggagca acaacaggct
     2041 ccacggatat ctgcttgaaa cccatttc
//
LOCUS       DAONVY010000428         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36466_length_2057_cov_3.585914, whole genome shotgun sequence.
ACCESSION   DAONVY010000428 DAONVY010000000
VERSION     DAONVY010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36466_length_2057_cov_3.585914"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2057
                     /locus_tag="PLI11_09835"
     CDS             <1..>2057
                     /locus_tag="PLI11_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPZ53208.1"
                     /translation="IRTLVEFILRYGDITTSDKPGQNVERAQYGAHIHKKLQQEFEKE
                     KDYNKEAYVRHTYEKSDISLTVTGRADGWYITDDKLYVDEIKTVEFDLEIMEEIDPLH
                     LAQAMCYAYVLSLDEKMNSIVNVIYYNIHTDEKRIMQKEYTFAELEEFFLNLCERYIS
                     WISFDRERKVKLHVQLKELKFPFPMYREGQRQLCTAVYRTIERENKLLVQAPTGIGKT
                     ISVLFPSLKAVAEGKGGRIFFLTARNAGVLAPQDTLLMLNSKANDLSFIALTAKEKIC
                     PFELACNPEDCEYAKGHYDRINDAVFESINNYRLFTRDVIEKISKKHKVCPFEFSLDV
                     SEWVDVVIGDYNYLFDPRVYLKRFFSSKQDHVFLVDEAHNLIDRTRDMYTQKLYKKSF
                     SEVRKKFKDRENGRNVYNKAGKVIRYFNKMNKSLDDKDYVVTEEFDNTMYMLVNDFTV
                     CLTEFLHKVGSDDEKVTELFMNCLFFCKLLEIYDERFCYYTRKENSDIFACVYLADPS
                     KIIGETLELGRSAILFSGTLEPLQYYYQGFGVKEDDKIFSVSSPFPKENRKVFICTDV
                     STRYKKRAFYYELIAEYILGLASLQKGNFIVYFSSYRYLKDVANCLPAELMDITVFIQ
                     PQTQDPDEREQFVHDFKSEPDGVRIGLCVLGGIYSEGIDLAGDRLGGVIVVGVGLPQV
                     CLE"
BASE COUNT          677 a          333 c          456 g          591 t
ORIGIN      
        1 ataagaacat tggtggagtt tattctcaga tacggagaca taacaacaag cgacaagccg
       61 ggccaaaatg ttgaaagagc ccagtatggt gcccatatcc ataaaaaact tcaacaggaa
      121 tttgaaaaag agaaagatta caacaaagaa gcgtatgtaa ggcataccta tgaaaaaagt
      181 gatatatccc ttacagtcac cggtcgtgca gatggctggt atataactga tgacaaattg
      241 tacgtggatg aaataaagac tgtggaattt gatttggaga taatggagga aattgatcca
      301 ttgcatctgg ctcaagccat gtgctatgca tatgttctct cattagatga aaagatgaat
      361 tcaatagtta atgttatcta ctacaacatt cacactgatg agaaaagaat aatgcaaaaa
      421 gagtatactt ttgccgaact tgaggaattc tttctaaatc tctgcgagag atacatatcc
      481 tggatttcct tcgacaggga aagaaaagta aaattacatg tgcagttaaa agaattgaag
      541 tttccttttc ccatgtacag agaaggacag agacaactgt gcactgctgt atacaggaca
      601 atagaaaggg aaaacaagct tcttgttcaa gctcctaccg gtataggaaa aaccatatcc
      661 gtattgtttc catctttgaa agctgtagct gaaggaaagg gcgggagaat attttttctc
      721 actgcgcgaa acgcaggagt tcttgcccct caggatacgc tattgatgct taattcaaaa
      781 gcaaatgatt tgtcatttat agcgctgaca gcaaaggaga agatatgtcc ctttgagctt
      841 gcatgtaatc cagaggattg cgaatacgca aaaggacatt atgacagaat aaatgatgcg
      901 gtatttgaaa gcatcaataa ttaccgccta tttacaagag atgttattga aaagatatcg
      961 aaaaaacata aggtgtgtcc ttttgagttt tccttggatg tttccgagtg ggtggatgtt
     1021 gttattggcg actacaacta tctttttgac cccagggtct acttgaaacg cttcttttca
     1081 agcaagcagg accatgtttt tttagtagat gaagcgcaca acttgattga cagaactagg
     1141 gatatgtata cccagaaact gtataaaaag tcgttttcag aggttaggaa gaagtttaaa
     1201 gacagagaaa atggcaggaa tgtatacaac aaagcaggca aagttattcg ttattttaat
     1261 aaaatgaata aaagccttga cgacaaagat tatgtcgtaa ccgaagagtt tgacaatacc
     1321 atgtacatgc tcgttaacga cttcacggtc tgtttgacgg agtttcttca taaggtgggt
     1381 agtgacgacg aaaaagtaac agagcttttc atgaactgtc ttttcttctg caaactttta
     1441 gagatatatg atgaaaggtt ctgctattac acacgaaaag aaaatagtga tatttttgct
     1501 tgcgtgtatc ttgctgatcc ttcaaaaata attggggaga cactggagct tggaagaagt
     1561 gcaattttgt tttctggtac attggaacct cttcaatatt attatcaagg gtttggagta
     1621 aaggaagatg acaaaatatt ttccgtttcg tctccgttcc caaaagagaa cagaaaggtc
     1681 ttcatttgca cggatgtttc gacccggtac aaaaaaaggg cattttatta tgaattgatt
     1741 gcagaatata tactggggtt ggcatctctt caaaaaggaa acttcatagt ttacttcagt
     1801 tcatacagat atttaaagga tgtagcaaat tgtcttcctg ccgagcttat ggatataact
     1861 gtgtttatac aaccccagac acaggatcct gatgaaagag aacagtttgt ccatgatttc
     1921 aaatcagaac cggatggtgt caggataggt ctttgtgttc ttggaggaat atattccgaa
     1981 ggtattgacc ttgcaggtga cagacttggt ggggtaattg tggttggagt tggactgcct
     2041 caggtatgct tggaaag
//
LOCUS       DAONVY010000429         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36471_length_2057_cov_2.459041, whole genome shotgun sequence.
ACCESSION   DAONVY010000429 DAONVY010000000
VERSION     DAONVY010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36471_length_2057_cov_2.459041"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLI11_09840"
     CDS             complement(<1..435)
                     /locus_tag="PLI11_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53209.1"
                     /translation="MKDIFEKKDIKPMLLTERKESFNSPDYLYELKLDGIRTIMYIDK
                     GSVEIRNKRNMRLNHTYPELADIYEQVRQKCILDGEIIAVKDGKPDFYAIQKRSMMTN
                     RTKIEFASKMYPVSFSAFDILYIGTEQITDKPLVERKQILSEN"
     gene            complement(437..1207)
                     /locus_tag="PLI11_09845"
     CDS             complement(437..1207)
                     /locus_tag="PLI11_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HPZ53210.1"
                     /translation="MAVAHKGAISFGLVHIPVNLYVAAERTGITFNQLHKECMGRIRY
                     KKSCPTCNLDEVSKDDVVKGYEYEKNKYIVIEEEELEKLKTEKDKTIYILNFVSLSEI
                     DPIYYEKTYYVAPDGSDRAYMLLAEAMKNEGKVAIAKTVMGTKEKLLTIRSTGAGLLI
                     ETMFFEEEIKTMPRPIGDVNLNEQELQMARILINNMTAEFKPEKYKDEYTMRMKEAIM
                     RKVNGAEIVQPQQEGAGAIANLMEALQQSIKATEQWVQ"
     gene            complement(1313..2038)
                     /gene="spoIIR"
                     /locus_tag="PLI11_09850"
     CDS             complement(1313..2038)
                     /gene="spoIIR"
                     /locus_tag="PLI11_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HPZ53211.1"
                     /translation="MKKVKHLLLVLVLIITAVCILNVYSNNVMEDLKGNLVRLHVVAN
                     SDNADDQDLKLSVRDSVVEYTNKILESCESSQEAYKILDRNLYEIERIARDAVRSKGY
                     DYDVDVSLGIFEFPTKDYGNIILPNGEYTSLKITIGEGLGQNWWCVLFPPLCFVNANV
                     NAVVNEPDADNDDNNREDGKVNEDEITEQGKEALKENLSQTSYEVIEKSNKGVKFKFK
                     IIEVIENAKMKIQATFKRALKLR"
BASE COUNT          493 a          487 c          334 g          743 t
ORIGIN      
        1 gttttcactc aagatttgct ttctttccac caacggcttg tccgttattt gttctgtgcc
       61 aatatataat atatcgaacg cagagaaaga aactgggtac atttttgaag caaattcaat
      121 ttttgttctg tttgtcatca tggatctttt ttggattgcg taaaaatccg gcttgccatc
      181 cttgaccgct ataatttctc catccagtat gcatttttgc ctcacctgct catatatatc
      241 cgccagttcc ggatatgtgt gatttaaccg catgtttctt ttgttcctaa tttcaacgct
      301 tcccttgtcg atatacatga ttgtgcgaat tccatccagt ttcagttcgt agagatagtc
      361 aggggagttg aaagactctt tcctttctgt aagcagcata ggttttatat ctttcttttc
      421 aaatatgtct ttcaacttac tgcacccact gttccgtagc ctttatgctt tgctgcagcg
      481 cttccataag attggcgatg gctccagcac cttcctgctg aggctgtacg atttcagcgc
      541 catttacctt tctcatgatg gcttccttca tcctcattgt gtattcgtcc ttgtactttt
      601 caggtttgaa ttcagctgtc atgttgttta tcaatattct ggccatttgc agctcctgct
      661 catttagatt gacatcacct attggccttg gcatggtctt tatttcctct tcaaaaaaca
      721 tggtttcaat aagcaaacct gctcccgtcg accttatagt cagaagcttc tcttttgtac
      781 ccattacagt tttggcaatg gcaaccttgc cctcattttt cattgcctca gccaggagca
      841 tatacgcacg gtcactgccg tccggggcta cgtaatatgt tttctcatag tatattgggt
      901 ctatctctga caaagataca aagttcaaga tgtatatggt cttgtctttt tctgttttga
      961 gtttttccaa ctcttcctct tctattacaa tatacttgtt tttctcatac tcataccctt
     1021 ttacaacgtc atccttggat acctcatcca ggttacacgt cgggcatgac tttttatatc
     1081 ttatccttcc catgcattct ttgtgaagct ggttaaacgt aatgccagtt ctttctgctg
     1141 caacatacag gttcacaggt atgtgcacca atccaaatga aattgctcct ttgtgagcca
     1201 cagccataaa aataccccct ttcgctttat tattagcaaa agaggacaat aaatacacaa
     1261 aaagaaaaga accaggtttt attaccggtt ctttcatatg cgttgttttg catcatctta
     1321 actttagagc ccttttgaaa gttgcctgta ttttcatttt ggcattctca ataacctcga
     1381 ttatcttgaa cttgaacttt actcctttat tggacttttc aataacttcg taggaagttt
     1441 gggacaaatt ctctttcagt gcttcttttc cttgttcagt tatctcgtct tcattcactt
     1501 ttccatcttc tctgttattg tcgtcattgt ccgcatccgg ttcatttaca actgcattta
     1561 cattcgcatt cacaaaacaa agaggaggga acaagacgca ccaccagttc tggccaaggc
     1621 cttctcctat ggtgattttc aaggaggtgt attctccatt tggcaatatt atgttgccat
     1681 aatcctttgt cggaaactca aatatgccaa gtgaaacatc cacatcataa tcataaccct
     1741 tgcttcgcac agcatccctg gcaattcttt caatctcata cagatttcta tccagtatct
     1801 tgtatgcttc ctgtgaactc tcacaggatt ccaatatttt gttagtatac tcaaccacac
     1861 tgtctcttac gcttagtttt aagtcctggt catctgcatt gtcactgttg gcaaccacat
     1921 gcagcctgac taggttccct ttcaagtcct ccattacatt gttggaatag acattcaata
     1981 tgcaaacagc tgtgattatt agtacaagga caagcagcag atgctttacc tttttcatta
     2041 caatacctcc atgtctt
//
LOCUS       DAONVY010000430         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36844_length_2041_cov_2.470292, whole genome shotgun sequence.
ACCESSION   DAONVY010000430 DAONVY010000000
VERSION     DAONVY010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36844_length_2041_cov_2.470292"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLI11_09855"
     CDS             complement(<1..364)
                     /locus_tag="PLI11_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HPZ53212.1"
                     /translation="MRCLLSIDWDYFIKTPQDYLNSYIECQKSIHDLWYKRYFKLKVR
                     GIDIKDLFKLDDYTVFWDKLKEKFRIGRNTRMIVSDSHKLSYNIAQRAKCKEVYLFDS
                     HADLGYGGLASLEFETNCA"
     gene            complement(411..1421)
                     /locus_tag="PLI11_09860"
     CDS             complement(411..1421)
                     /locus_tag="PLI11_09860"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPZ53213.1"
                     /translation="MIVDDSLFFREMLSAKLASYSNLEVVATAADPYDAVEKIKMYKP
                     DVLTCDIEMPKMNGIEFIRQLMPQYPIPVVVVSSVSNVVFKALHAGAVDFIAKPKAGN
                     KEELDLFVNELVGKIRIASMAKVPYNNEELVLEKEHMNDITKSNIDLIAIGASTGGTE
                     AIRQILKELPKDMPGIVIVQHIPPVFSKMFADGLNETTGFKVKEAEDRDIIKPGHVYV
                     APGDFHTRVSKLGSVYRVNCVKEDKVNNHRPSVDVLFESVAKICKGNAIGVILTGMGK
                     DGAMGLLSMKKNGNFTIGQDEESSVVYGMPKAAYDIGAVVKRVPLNKVASTIVSSLSG
                     KM"
     gene            1579..1872
                     /locus_tag="PLI11_09865"
     CDS             1579..1872
                     /locus_tag="PLI11_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53214.1"
                     /translation="MTIEIHEALCQEFKEHIGETVTVYTTSGGRSGVGFTGLLTHANC
                     YFIRLVKHLRPVVCPHFDYRKKKCYTCKQYKFKGVTTIIPMDKIAAFVHSTKS"
BASE COUNT          623 a          466 c          349 g          603 t
ORIGIN      
        1 tggcacagtt tgtctcaaac tccaaagaag caagaccccc ataacccaaa tccgcatggg
       61 agtcaaacaa atacacttcc ttgcattttg ctctttgtgc gatattgtac gacagtttgt
      121 gagagtctga gacaatcatt cttgtattcc tgccaatacg aaacttttct ttgagcttgt
      181 cccagaacac agtatagtca tccagcttga aaagatcctt tatgtctatt cctctaacct
      241 ttagtttgaa atatcgtttg taccataaat catgaatgct tttttggcat tcaatatatg
      301 agtttagata atcttgaggg gtcttgatga aatagtccca atctattgac aagagacatc
      361 tcataatccc gcaccttctt tgttcccaca atatttatat atacattata tcacatttta
      421 ccagaaagtg aggaaacaat tgtcgaagca accttgttca aaggcaccct ttttacaacc
      481 gcccctatgt catatgcagc tttgggcatg ccgtatacaa cgctgctctc ttcatcctgt
      541 cctatggtaa agttgccgtt tttcttcatg gatagaagcc ccatcgctcc atccttgccc
      601 attccagtca gaattactcc tattgcgttc cccttgcata tcttggcaac cgactcaaac
      661 aaaacgtcaa cagaaggcct atgattgttg accttgtctt ccttaacaca gttaaccctg
      721 tatacacttc caagcttgga gaccctcgta tggaaatccc caggtgcaac atacacatgc
      781 cccggtttta taatatccct gtcctcagct tccttgactt tgaaacctgt agtttcatta
      841 agaccatctg caaacatttt tgagaaaaca ggaggtatgt gctgaactat gacaatgccc
      901 ggcatatcct ttggaagctc cttcaatatc tgacggatcg cctctgttcc tccagtagat
      961 gcgccaatgg ctatcaggtc gatatttgat tttgttatat cattcatatg ctccttttca
     1021 agcaccagtt cctcattgtt gtatggaacc tttgccatgg aggcaattct tatcttccca
     1081 accagctcat ttacaaacaa atcaagctcc tctttattgc cagcctttgg tttggcaata
     1141 aagtctacgg cacctgcatg aagcgcttta aaaaccacat tgctgactga actgaccacc
     1201 acaacaggta tgggatattg gggcatcagc tgtcttatga attcaatgcc gttcatcttc
     1261 ggcatctcaa tgtcacaggt caagacatcg ggtttgtaca tctttatctt ttctactgca
     1321 tcataaggat cagcagcagt tgctaccact tccaggtttg aatatgaagc aagctttgca
     1381 gatagcattt ctctaaaaaa caaagaatca tcaactatca tcacttttat tacactaccc
     1441 acagaatact actcctttat atatggtatt caattaatta taccaaaagt actacagttt
     1501 tgaaatgaag caaattttaa caattgtgta caatattcca tattatataa tgacacttca
     1561 aattgttgag gcaaaacaat gacgatagaa atacacgaag ctctatgcca agagttcaaa
     1621 gaacacatag gagagacagt gacagtctac actacatcag gaggacgttc aggagttggt
     1681 tttacaggac ttctgacaca tgccaactgc tactttatta gactggtaaa acacctgcga
     1741 cctgttgtat gcccgcattt cgattacaga aaaaagaaat gttatacctg caaacaatat
     1801 aaattcaaag gagtaaccac cattatcccc atggataaaa tagcggcctt tgtacacagt
     1861 acaaaaagtt aacataaaac atgcactctc catataatat actgaatccg gaaatacacg
     1921 gatgaaaatg tataaggagg tgcaacatgt attttgcaga caatttgaag gatgacctga
     1981 tagaccatat tggtgaaaca ataactgtat tcacaaagag cggtggcgaa tcaggcagag
     2041 g
//
LOCUS       DAONVY010000431         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36882_length_2039_cov_2.971270, whole genome shotgun sequence.
ACCESSION   DAONVY010000431 DAONVY010000000
VERSION     DAONVY010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36882_length_2039_cov_2.971270"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLI11_09870"
     CDS             <1..636
                     /locus_tag="PLI11_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPZ53215.1"
                     /translation="SVLTTILVYMISRTGGRLPAATLLLTGVAFHFLMSAGINFLLMF
                     NREMADRVVLWNMGSFSNSSWTSVAITVPVVVVCSIIIIFNAKNLNILSVDEPTAKSL
                     GVDVERNKTMMLVVTALIVAVCVSSCGVVGFVGLVIPHITRILYRPDHRVLLPFSALF
                     GSLFLVLCDLVARNLLNWLRGFSSEMPVGAITSAFGAPFFIWLLIKKKRNV"
     gene            649..1425
                     /locus_tag="PLI11_09875"
     CDS             649..1425
                     /locus_tag="PLI11_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ53216.1"
                     /translation="MSILSVANVSFSYGEYEVLKNIDADFEEGKLYVILGPNGSGKTT
                     LLNCIMSNLAWYSGIIRLKGQDVKKMTSRERARLISYVPQNTVIDFEFTAREIVLMGR
                     SPYLRFFENESLEDVRIAEECMAMTNVLHLMDKNIKEISGGERQRVLIARALCQDTPI
                     MVLDEPASSLDIYHEINVMDILRNIVREKRRTVICVMHDLNTAARYADYLYLMSNGNI
                     VARGEAKEVFNSENLEKVYSVKSDVIEVDGKKYIVIDSIV"
     gene            complement(1428..>2039)
                     /locus_tag="PLI11_09880"
     CDS             complement(1428..>2039)
                     /locus_tag="PLI11_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53217.1"
                     /translation="ASLCAGIIAYIISKHMKSEKLYKQHMSGSKTQEKDKKKEAFFEM
                     LEYHSLPEKIHALELARKVSGLMSRQTDFTYLTALRQINDKLSNVNNPDNILCKMIST
                     IPGVFPQPKRRFYLKEEDGQIVFYDADFEDPFGEIICHKDDVVSFGEYVNYSKRVPNP
                     GGGKIRPESIIVELKDDNYNLFFEFKNHEYEDIRKIFGGKKAI"
BASE COUNT          565 a          364 c          459 g          651 t
ORIGIN      
        1 agcgttctta ctacaattct tgtatacatg ataagcagaa caggtggaag gcttcctgcg
       61 gcaacgcttt tgctgacagg tgtggctttt cattttctaa tgtctgcagg aattaatttt
      121 ctactaatgt tcaataggga aatggcggac agagtggtac tttggaacat gggaagtttc
      181 tcaaactcat cctggaccag tgtagccatt accgttccag tagttgtggt atgctcaata
      241 attatcatct ttaatgccaa gaatcttaac attctttccg tggacgagcc tacggcaaaa
      301 tcactgggtg tggatgtgga aagaaacaag accatgatgc ttgtagttac cgcacttata
      361 gtggcggttt gcgtgtcttc ctgcggtgtt gtgggttttg taggacttgt catacctcat
      421 attacgcgaa tactatatag gccggaccac agggtactgc ttccgttttc tgccttgttt
      481 gggtctttgt ttctggttct gtgcgatctg gttgctagaa accttttaaa ctggttgagg
      541 ggattttcca gcgagatgcc tgttggtgca ataacttcag cttttggggc gcctttcttc
      601 atatggctgc ttataaagaa aaaaagaaat gtttgagtgg aggattgtat gtccatacta
      661 agtgttgcca atgtttcctt ttcttatgga gaatacgagg ttttgaaaaa catagatgct
      721 gattttgagg aaggtaagct ctatgtaatc cttgggccta atggctcggg caaaacaaca
      781 ctactcaact gtataatgtc aaatctggcc tggtactcag gtatcattcg tcttaaagga
      841 caggatgtaa agaagatgac aagcagggag agagccagat tgatttccta tgtcccacag
      901 aatactgtaa ttgattttga attcaccgcc agagaaatag ttcttatggg aaggagcccg
      961 tatttaaggt tttttgagaa tgaaagtctt gaggatgtta gaatagctga agagtgcatg
     1021 gcaatgacca atgtccttca tctcatggat aagaacatca aggagattag tggcggtgaa
     1081 agacaaagag tcctgattgc aagagctttg tgccaggata cgcccatcat ggttcttgac
     1141 gagcctgcat ccagccttga tatttatcat gagataaatg taatggacat attaaggaac
     1201 attgtaaggg agaagaggag aactgtaata tgcgtcatgc atgatttaaa tacagctgcc
     1261 agatatgcag attaccttta ccttatgagt aatggcaaca ttgtggcaag aggagaagca
     1321 aaagaggttt tcaatagtga aaacctcgag aaagtatact ctgtcaagag cgatgttatt
     1381 gaagttgatg gcaagaaata tattgtaatt gacagtatag tataatccta aatagctttc
     1441 ttgcctccaa agatcttcct tatatcttca tattcatgat tcttaaattc aaaaaacaga
     1501 ttgtaattat cgtccttgag ttctacaatt atggattcag gccttatttt tccgccaccg
     1561 ggatttggaa ctctttttga gtaattgaca tattctccaa aggaaaccac atcgtccttg
     1621 tggcaaatga tttctccgaa aggatcttca aaatccgcat catagaagac aatctgaccg
     1681 tcctcttcct tcagatagaa tcttctcttt ggctgtggaa aaacacctgg gattgttgat
     1741 atcattttgc acagaatgtt gtcaggattg tttacatttg acagtttgtc gttaatctgg
     1801 cgtagtgctg taaggtaggt gaaatccgtc tgcctgctca tgagacctga cacctttctt
     1861 gccaattcaa gagcatgtat tttttcagga agactgtgat actcaagcat ttcgaagaag
     1921 gcttctttct ttttgtcttt ttcttgtgtt ttagaaccgc tcatgtgttg tttgtatagt
     1981 ttttcgcttt tcatatgttt tgaaataatg taagctatga tacccgcaca cagagaggc
//
LOCUS       DAONVY010000432         2038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_36901_length_2038_cov_3.003530, whole genome shotgun sequence.
ACCESSION   DAONVY010000432 DAONVY010000000
VERSION     DAONVY010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36901_length_2038_cov_3.003530"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLI11_09885"
     CDS             <1..323
                     /locus_tag="PLI11_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53218.1"
                     /translation="NPTEKRYNNDYCMSNSFTLTRRDMLLFYKVLRKFYKDGFRFYDL
                     YNIRRILQSEGFNWYKLRKALDVFIELDLVRRDTKEIYTLNLDAEKVELDTSHLYKAL
                     NGER"
     gene            357..>2038
                     /locus_tag="PLI11_09890"
     CDS             357..>2038
                     /locus_tag="PLI11_09890"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPZ53219.1"
                     /translation="MNDNAKLEQLINMILSYNPGADIALLEKAHAFATERHAAQKRKS
                     GEAFIVHPESVAIMLAEMEMDLPTIIAGMLHDVIEDTSTTFEEVESEFGHEIAYLVDG
                     VTKLKRIPTTTKEELQAENLRKMFLKMADDIRVILIKLVDRLHNMRTLQYLNTDSQIT
                     KARETLEIYTPIAHRLGITKIKWELEDLCLKYLEPETFENLQNQIKQKREKQEEYIKL
                     ILDKIKDRTQEININAYLDGRPKHLYSIHKKMVRQGKTMDQIYDLIAVRVIVDTVKEC
                     YMVLGIIHELFKPMPGRFKDYIAMPKPNMYQSLHTTLIGPDGLPFEAQIRTWEMHKVA
                     EIGIAAHWKYKGGKSDPEFENKIEWIRNLLNLQNDTRDPSDFVETFKMDLFTDEVYVF
                     TPKGDVISLPAGSTPIDFAYAIHSAIGNRMIGAKVNSEIKPLTYKLKTGEIVEIITTK
                     DPNHGPSRDWLNIVKSSEAKSKIRQWFKKERREENIERGKSMIEKELKKQGYTHAQLF
                     KTEWLEALLKRYNMNTLDDLYNVIGFGEMSAQKVVSRLRDEYLKTVTDKEELA"
BASE COUNT          726 a          356 c          470 g          486 t
ORIGIN      
        1 aaaatccaac agaaaaaagg tataataacg attattgtat gagcaacagt tttacgctta
       61 caagacgtga tatgctgctg ttttacaagg tgttaaggaa attttacaaa gatggattta
      121 ggttttatga cttatacaat ataagaagaa ttttacaaag tgaaggattc aactggtaca
      181 agttaagaaa ggctcttgat gtatttatag agctggattt ggttagaaga gacacaaaag
      241 agatttacac tttgaatttg gatgctgaaa aagtggaact tgatacatcc catttataca
      301 aagcattaaa tggtgaaaga taagcaatac taaaaagtag cagaggaggt agcgcaatga
      361 acgataatgc aaagttggaa cagttgataa atatgatatt gtcgtacaat cctggagcag
      421 acatagcctt gcttgaaaag gcgcatgcgt ttgcaacaga aagacatgct gcccaaaaaa
      481 gaaaatcagg cgaagcattt atcgttcatc cggagtcagt agctattatg ctggctgaaa
      541 tggaaatgga tttgcctacc ataattgctg gaatgctgca tgatgtgata gaagacacct
      601 caacaacttt cgaggaagtt gagtctgaat tcggtcatga aatagcatat ctggtggacg
      661 gggttacaaa acttaaaaga atccctacca ccacaaaaga agagcttcag gctgaaaatc
      721 ttcgaaagat gtttctcaag atggcggacg atattcgtgt catcctcata aaactggtcg
      781 acaggcttca caacatgaga acccttcaat atttgaatac tgattcacaa ataacgaagg
      841 caagggagac tttggagata tatactccaa ttgcccatag gctcggtata accaagatta
      901 aatgggaact tgaggatttg tgtcttaaat atctggagcc ggagaccttt gaaaatttac
      961 aaaatcaaat caagcagaaa agagaaaagc aggaagaata tataaagctt attctcgata
     1021 agataaagga tagaactcag gagataaaca taaatgcgta tcttgacgga aggcccaaac
     1081 acctttacag cattcacaag aaaatggtcc gtcaggggaa aaccatggac cagatatatg
     1141 acctcatagc tgtcagagtg attgtggata cagtcaagga atgctatatg gtgttgggaa
     1201 taattcatga gctgttcaag ccaatgccag gcagattcaa agactacatt gccatgccta
     1261 aacccaacat gtatcagtca ctgcatacaa ccctcattgg tcctgacggg cttccttttg
     1321 aggctcagat aaggacttgg gagatgcaca aggtggcgga gattggaatt gcagcccact
     1381 ggaagtacaa gggcggaaaa agcgatcccg agtttgagaa caagatcgag tggataagaa
     1441 accttcttaa tctgcagaac gatacaaggg atcccagtga ttttgtggaa accttcaaga
     1501 tggacctatt cacagacgag gtttatgtgt ttacgccaaa aggtgatgta ataagtttgc
     1561 cggcgggttc cacacccata gactttgcct atgccattca cagtgcaatt ggaaacagga
     1621 tgattggcgc aaaagtcaac agtgaaataa agcctttgac atacaaactg aaaaccggtg
     1681 aaattgtcga gataataacc accaaggatc caaaccacgg tccatcaagg gattggctga
     1741 acatagtaaa gagttcagaa gcgaaaagca agattcgaca atggttcaag aaggaaagaa
     1801 gagaagagaa tattgagcgc ggcaagtcaa tgatagaaaa agagctcaaa aaacaggggt
     1861 acacccatgc ccagctcttt aaaaccgagt ggcttgaagc tttgttgaaa agatacaata
     1921 tgaacactct tgatgacttg tacaatgtca ttggatttgg tgaaatgagt gcccaaaagg
     1981 tcgtgtcaag actcagggac gagtatctga agacggtaac cgacaaggaa gaacttgc
//
LOCUS       DAONVY010000433         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37055_length_2031_cov_2.500000, whole genome shotgun sequence.
ACCESSION   DAONVY010000433 DAONVY010000000
VERSION     DAONVY010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37055_length_2031_cov_2.500000"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2031
                     /locus_tag="PLI11_09895"
     CDS             <1..>2031
                     /locus_tag="PLI11_09895"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPZ53220.1"
                     /translation="FKRMAFRILSRYGGLAKTKLNPSGRIMLLGHVVKENIDRLEYFE
                     FLEERPGEINRLLGLIDEFGRYEVTPGMLEEATLELEDDLLKSKLSDLSLIYSEYRNL
                     LLEDNIEDQDIYSAFLEKLAEHRPFKDAVIWLDGFAGFTSYEFDILKELVRQCRDVNV
                     CLCMDEEDEYIFDRVYRTYEQLLRVAKDVDARINITYLKESHRFTNKYIAHLERNFGK
                     YPAKRVQDIPKNIKIVECRSIYEEVDRSAREIVRLLREEGLRYKDIAVTARSIDLYEK
                     VIKIVFPKYNIPYFIDSKKNIEDHPLIRFILTLLDIVGSSWERTSVLDFLKTGLYTEE
                     ICVVDRFENIALAHGLRGKKDFGSSIDEFEEVEVLRAKLYSDVTSFEEELKKCRTMKE
                     CCKILVSFFSKLDVQGKMQNMSLKFKESNMPVMANEYARIWNITMEVVEQIYVFMGDI
                     RLTGSLKDKVKLFKETLLLGFSEYKIGFIPFTEDSVQIGVADRTRSHDVKGMILLGAN
                     EGVFPATFVDEGLLKDDDRAYLKNKGIALAEDNRTKANMEYYLIYTVLSSPSEHMIIS
                     YALEDHGLNPMRPSVIVQRIQNIFPSLKVVKDYNEGVMERLTLPLPFLTENAGLLRDV
                     EDETIKCIRKWYRDSAEYKHEFERILKADTSFRRDLYLPKDIMDELFARNLYM"
BASE COUNT          636 a          315 c          515 g          565 t
ORIGIN      
        1 ttcaaacgca tggctttcag gatattgtcc aggtatggag gacttgcaaa gacaaaactg
       61 aatccttcag gccgtattat gttgcttggt catgtggtga aagagaatat tgataggctt
      121 gaatactttg agtttctgga agaacgtcca ggagagatta acaggctttt aggtctgatt
      181 gatgagtttg gcagatatga agtcactccc ggcatgcttg aggaggcgac attggagctt
      241 gaagatgatt tgttgaaaag caagctttcc gatttgtccc taatttacag tgagtacagg
      301 aatcttcttt tggaagacaa tatagaggac caggatattt acagtgcgtt tttggaaaag
      361 cttgcagaac acagaccttt caaggatgct gtaatatggc ttgatgggtt tgcaggtttt
      421 acatcctatg agtttgatat attgaaggag ctggtcaggc agtgcaggga tgtaaatgtg
      481 tgtctttgca tggatgagga agacgagtac atattcgaca gggtgtacag aacgtatgaa
      541 cagctgctaa gggtggcaaa ggatgtcgat gcaaggataa acattacata cttgaaagaa
      601 agtcatcgct ttactaacaa atatattgcc catcttgaaa ggaactttgg caaatatcct
      661 gcaaagaggg tgcaggacat acccaaaaac ataaaaattg ttgaatgcag aagcatatat
      721 gaagaagtgg atcgcagtgc aagagaaatt gtgaggcttt tgagggagga aggtttaaga
      781 tacaaggata ttgcagtaac tgcaagaagt attgatttgt acgagaaggt tataaagatt
      841 gtgtttccaa agtacaatat tccatacttc attgactcca aaaagaacat agaagatcat
      901 ccactaatta gattcattct gacattgctt gatattgtgg gaagttcctg ggagaggact
      961 agcgtgctcg attttctgaa aactggcctg tatacggaag aaatctgtgt ggttgacagg
     1021 tttgagaata ttgcgcttgc ccatgggcta agaggcaaaa aggattttgg ttcaagtatt
     1081 gatgagtttg aagaagttga agtgcttcga gcaaagttgt atagcgatgt aacctcgttc
     1141 gaggaagagt tgaaaaaatg ccgcacaatg aaagagtgct gcaaaattct tgtttcgttc
     1201 ttctccaaac ttgacgtcca gggaaaaatg cagaacatgt cattgaagtt caaggaatcc
     1261 aacatgcctg ttatggcaaa tgaatatgca agaatctgga acataaccat ggaagttgta
     1321 gagcagattt acgtgttcat gggtgacata aggttgacag gttcgttgaa ggataaggta
     1381 aagcttttca aggaaacgct tttattgggg ttttccgagt acaagattgg ttttattccg
     1441 tttactgaag acagtgtcca aattggtgtt gcggacagaa caagaagcca tgatgttaaa
     1501 ggaatgatac tgctgggcgc aaacgaggga gtttttcccg caacctttgt ggatgaaggt
     1561 cttttgaagg acgacgacag agcgtatttg aagaacaagg gaatcgctct tgcagaggac
     1621 aacaggacaa aggcaaatat ggagtattac cttatttaca cggtcttgtc atctccctcc
     1681 gagcatatga taattagtta cgcattggaa gaccacggac tgaatcctat gagaccgtcg
     1741 gtaattgttc agaggataca gaacatcttc ccgtctttga aggttgttaa ggattacaac
     1801 gagggagtaa tggaaagact tactttgcct cttccttttt tgactgagaa tgcaggactt
     1861 ttgagagatg tagaggacga gacaatcaaa tgtatcagaa aatggtatcg ggatagtgct
     1921 gagtacaaac atgaatttga aagaattttg aaagctgata catctttcag aagagacttg
     1981 tatctgccaa aagacataat ggacgagctt tttgccagga acctttacat g
//
LOCUS       DAONVY010000434         2019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37337_length_2019_cov_2.922607, whole genome shotgun sequence.
ACCESSION   DAONVY010000434 DAONVY010000000
VERSION     DAONVY010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37337_length_2019_cov_2.922607"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLI11_09900"
     CDS             <1..707
                     /locus_tag="PLI11_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPZ53221.1"
                     /translation="KDYKKILSDTWESGKELSYSFDTVKARYVKIRVDEAVKVSDIYI
                     AEICEIEIYNDDGTLPKYQRVWEKDKTLIPEYNVALNKRVKTSSSLEAPQWGWMRKHI
                     NDGMIKATNTHSGWTTQTGRHMTDPYAEEWVLIDLGEKFNIDTVVLYPRQDTGYYFPK
                     HLVVEVSLDEKDWTEVYELKESGAVSTIARVLKFDAVDARYVRVVSKEMTQVESSPDG
                     YLFQLAEFEVYRTGRQ"
     gene            complement(773..1288)
                     /locus_tag="PLI11_09905"
     CDS             complement(773..1288)
                     /locus_tag="PLI11_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HPZ53222.1"
                     /translation="MKFSFKRNTKKFAEVVSKRNPGAYADILGDREHQSIVGRVDFYQ
                     VRNGVLLVAEIHGLPTKPDICASKVFGFHIHEGEECSGDKEDPFRNVGMHYNPDRCTH
                     PQHAGDLPPLFENNGYAWMAFYTNRFRLFEVIGRTIIVHQLPDDFTTSPSGSAGAKIA
                     CGEIKRYIPRK"
     gene            1417..1977
                     /locus_tag="PLI11_09910"
     CDS             1417..1977
                     /locus_tag="PLI11_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004138888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPZ53223.1"
                     /translation="MKKGFTLVEIIVVITIIAIIAAIAVPSVVQYYKYSESRNRNSVA
                     RTLYVAATNSLTQKYIAGLLNELPYDGKVNLENLITDDENFYDDEIYYNNNTENIVYV
                     TSKENVSRILDGYIMDTSVLNNSILIEYNIVTGKVLSVLYSDKVDAFGYGDGNFTDVL
                     DRTKAAREEKRFGFHGARSTGIPESE"
BASE COUNT          628 a          363 c          453 g          575 t
ORIGIN      
        1 gaaaagatta caagaaaatc ttgtcggata cctgggaaag cggaaaagag ctcagttatt
       61 cctttgatac agtaaaggca aggtatgtga aaattcgtgt tgatgaagct gttaaggtgt
      121 cggatatata tattgcagag atatgtgaaa ttgagattta caatgatgat ggtacactgc
      181 caaaatatca gagggtatgg gaaaaagaca agacccttat accagagtat aatgtagcgc
      241 ttaacaagag ggttaagacg tcatcatctt tggaagcacc ccagtgggga tggatgagaa
      301 aacacataaa tgatggcatg attaaggcaa ccaatacgca ttcaggctgg accacacaga
      361 cagggcgtca catgaccgac ccttatgctg aagagtgggt actgattgac ctgggtgaga
      421 agtttaacat tgataccgtg gtattgtatc caaggcagga tacgggctat tacttcccaa
      481 aacatcttgt tgttgaggtt tcgttggatg aaaaagactg gacagaggtg tatgaactaa
      541 aggaatccgg tgctgtttca accattgcaa gagttttaaa gtttgatgca gttgatgcac
      601 gatatgtaag ggtggtttca aaagagatga ctcaggtgga atcgtcgcct gacggctatc
      661 ttttccaact ggcggagttt gaagtctata gaactggaag gcaataatca tgataaagca
      721 taacaaagag gcaagcagat atgcaagcct ctttgttatt tatatcaagc aattacttgc
      781 gtgggatata cctttttatc tctccacatg ctatttttgc gccagcactt ccagagggag
      841 aggttgtgaa gtcatcgggc agttgatgca caattattgt acgcccaata acctcaaaca
      901 atctgaatct gtttgtgtaa aatgccatcc aggcgtatcc gttgttttca aaaagcggtg
      961 gaaggtctcc tgcatgctgc ggatgggtgc atctgtcagg gttgtagtgc attcctacgt
     1021 ttctaaacgg atcttccttg tcgcctgaac attcttcacc ctcatgtata tggaatccaa
     1081 aaaccttgct ggcgcaaatg tctggttttg ttggcaaacc atgtatttcc gcaaccagca
     1141 gaacaccgtt tcttacctga tagaaatcta cccgtcccac tatgctctgg tgttctctgt
     1201 cgcctaaaat atccgcatag gctccagggt ttcttttaga aactacttcg gcaaattttt
     1261 tggtattcct cttgaaactg aatttcataa tttcacctct tttcctctat ataatgtatg
     1321 aaaaaatgta tgcatttgtg agaaccttga aattaagtga tttctttagt atacttaagt
     1381 atacttacag aaaaatagaa gttggcgatg aacatcatga aaaaggggtt tactctggtt
     1441 gagataattg ttgtaattac catcatagcg ataatagcag cgatagccgt tccttcagta
     1501 gtgcaatact ataaatactc tgagagcagg aacagaaata gtgtcgccag aactctttat
     1561 gttgccgcaa ccaacagtct tacccaaaaa tatatcgccg gacttcttaa tgagttgcct
     1621 tatgatggaa aagtcaatct tgaaaacctg ataactgatg atgagaattt ctatgatgat
     1681 gagatatact acaataacaa tacagaaaac attgtgtacg ttaccagcaa ggagaatgta
     1741 agcaggatcc ttgatggata cataatggac acaagtgttc ttaacaactc catccttatt
     1801 gaatacaata ttgttacagg aaaggttctg tcagtcttat attctgacaa ggtagatgcc
     1861 tttggatatg gggatggaaa ctttacagat gttttagaca ggacaaaggc agctagagag
     1921 gaaaagagat ttggattcca cggggcaagg tcaaccggaa tcccggagag tgagtaaaac
     1981 tatgtttaaa catgtacgta ataattcagg tgcttcaat
//
LOCUS       DAONVY010000435         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37437_length_2015_cov_2.785714, whole genome shotgun sequence.
ACCESSION   DAONVY010000435 DAONVY010000000
VERSION     DAONVY010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37437_length_2015_cov_2.785714"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLI11_09915"
     CDS             <1..318
                     /locus_tag="PLI11_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase,
                     release factor-specific"
                     /protein_id="HPZ53224.1"
                     /translation="SLPDIKFDYILSNPPYIAKEEIRTLEPQVKDFEPEIALDGGLDG
                     LDAYKKIISQAKKYLSGSLLLEAGINQAQYIANEMEIHSYSDIFTVKDLNNIDRVVHG
                     LMN"
     gene            complement(308..>2015)
                     /locus_tag="PLI11_09920"
     CDS             complement(308..>2015)
                     /locus_tag="PLI11_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPZ53225.1"
                     /translation="WVKFYGKDTPPNLEYSDKTMFGVFYDTATQYSDYNAIDYMEKKI
                     TYGELLEEVKRCAASLAKLGIKKGDRITLCLPNIPQAVISFYATLALGAVANMIHPLS
                     ASNEIEYFINVSKSKMLFILDAFYEKTKDLNCETLQKVVTASAADYLSAVKKVGFALT
                     QGRKIPKVGASEKTMTWVGLMKLSEGVETPKIDEHPDNKYSDTAVILYSGGTTGTQKG
                     IMLSHLNFNALAAQTVAHGGPSFKPGDSMLAVLPMFHGFGLGVCIHTSVYKGATMILV
                     PRFNADSFAELIVKKKPTFMAGVPTLYEALLRKDRLAKADFSETKGIFSGGDKLPHDL
                     KVRFDEFLKEKNAKVTLREGYGLTECVTASALNPEGAAYRKGSIGIPYPDTLYKIVEP
                     GTTNDVPYGTDGEICIHGPSVMQGYLDDPEETATVLKKHPDGRIWLHTGDLGYIDEDG
                     FVYFKLRIKRMIKTSGYSVYPTQIEEILNAHEAVSMSCVIGVPDDLKIQTIKAFIVLK
                     EGYAPSEEMEKEIMRYCEENIAKYQIPRIIEFRDDLPTTKVGKIAYRVLEEEHLNLAA
                     SS"
BASE COUNT          540 a          462 c          373 g          640 t
ORIGIN      
        1 agcttgccgg atatcaaatt cgattatatt ctttccaacc cgccatatat tgcaaaagaa
       61 gaaattagga cgctggaacc tcaggtaaag gactttgaac ctgaaattgc ccttgatggt
      121 gggcttgatg gtcttgatgc atataagaaa atcatatcac aggcaaaaaa atacttatcc
      181 ggctccctac tcctggaagc cggaataaat caggcacaat atatagctaa tgaaatggag
      241 atacactcct attcagacat cttcacagta aaagatctaa acaatataga cagagtagtt
      301 cacggtctta tgaactagcc gccaggttta aatgctcttc ttcaagaacc ctgtaggcaa
      361 tcttgcctac tttggttgtc ggcaaatcat ccctgaactc gataatccta ggaatctggt
      421 attttgcgat attctcctca caatacctca taatttcctt ttccatttcc tcgcttggtg
      481 cgtatccttc cttaagcaca ataaatgcct ttatagtctg tattttcaaa tcatcaggaa
      541 caccaatcac acaggacatg gataccgcct catgtgcatt cagtatttct tcaatctggg
      601 ttgggtaaac cgagtatccg gatgtcttaa tcatcctctt tattctaagt ttgaaataga
      661 caaaaccatc ttcgtctatg tatcccaggt cacctgtatg gagccaaatg cgtccatcgg
      721 gatgcttctt aagaacagtt gcagtttctt caggatcatc cagatatcct tgcattacac
      781 ttggtccatg tatgcagatt tcaccgtctg ttccgtaagg aacatcatta gttgtacctg
      841 gttcaacaat cttgtacaaa gtatcgggat atggaatgcc tatgcttcct ttcctatatg
      901 ctgctccttc tggatttagg gcactagcag ttacacattc agtaagtcca taaccttctc
      961 ttagtgttac ctttgcattc ttttccttca ggaactcgtc aaaccttacc ttcaggtcgt
     1021 ggggcagttt gtcaccacct gagaatatgc cctttgtttc tgagaagtca gcttttgcca
     1081 acctgtcttt tctgagcaag gcttcgtaaa gagttggtac accagccata aaagttggtt
     1141 ttttcttaac aatcagttct gcaaatgaat ctgcattgaa ccttggcacc agaatcattg
     1201 tagcgccttt gtaaacactg gtgtgaatgc aaacacctaa accaaatcca tggaacattg
     1261 gcaatacagc cagcatggag tctccgggtt tgaagctggg tccgccgtga gcaacggtct
     1321 gagccgcaag agcgttgaaa ttcaaatgtg acagcattat tcctttttgt gtaccagtcg
     1381 ttcctccgct gtagagtatt actgccgtat cactatattt attgtccgga tgctcatcaa
     1441 tttttggcgt ttcaacgcct tctgacaact tcataagacc aacccaggtc atggtctttt
     1501 cactagctcc aaccttgggt atttttcttc cctgtgtcag agcaaaccct acctttttga
     1561 ctgctgatag ataatcggct gcactggctg tgacaacctt ctgcaatgtt tcacagttta
     1621 agtcttttgt cttttcgtag aaggcatcga gaataaacag cattttactc ttgcttacat
     1681 tgataaaata ctctatttca ttgctggcag ataatggatg aatcatgttt gccacggcac
     1741 ctaatgccaa tgttgcataa aaggagatga cagcttgtgg tatattgggc aaacacaaag
     1801 ttattctatc gccttttttt atgcctagct ttgccaaaga ggctgcgcat cttttaacct
     1861 cttccaacaa ttccccatat gtaatctttt tctccatata gtctatagca ttgtaatcag
     1921 aatattgcgt tgcagtatca tagaatactc caaacattgt tttatcgctg tattccagat
     1981 ttggcggtgt atctttaccg tagaacttga cccat
//
LOCUS       DAONVY010000436         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37541_length_2011_cov_3.243354, whole genome shotgun sequence.
ACCESSION   DAONVY010000436 DAONVY010000000
VERSION     DAONVY010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37541_length_2011_cov_3.243354"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..324
                     /locus_tag="PLI11_09925"
     CDS             142..324
                     /locus_tag="PLI11_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HPZ53226.1"
                     /translation="MSHKKCQSISCDVASCSFNDTLENSCTLSAIKVTPKMNCATEDI
                     DESMCASYKSRALVDR"
     gene            complement(449..>2011)
                     /locus_tag="PLI11_09930"
     CDS             complement(449..>2011)
                     /locus_tag="PLI11_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ53227.1"
                     /translation="FNNIKTKKFRTILTSFAASIGIIGIALILALSNGFQIEIDKFEE
                     NTMSGFPIIIGQQATTVDIADMGKKFGEENRKYKEFTDKKEVIPFDRSESELIHMNNI
                     TEEYIEYLKSIDPNLLLGTTYTRMTSLNLLKLDRDNVAKIVSMQDLDMSVLPKKFGEE
                     GFVEKNYDILYGKMPENKNELLLVVDKFNRLDKRVLETLGFSTYESIAFEDIIGTELR
                     AIPNNDYYVERDGFFTLVGNPADMTEIYNRETAIPLKIVGILRIKKDKMLDVIRTGVA
                     YSEELMEYILEDSENSDIAKAQREADYNVLTGQPFDLTTEKGKEDKENVLRVIGANSV
                     PLMITIFPRDFDSKDDVLAFLDAYNEGKDSKDRIIYTDMAEQITKLTKKIIDSISIVL
                     IAFASISLFVSSIMIGIITYISVLERTKEIGILRSLGARKSDITLVFNAETFIIGVFS
                     GLIGIAIASLLVIPTNMIIANYSNLENVAKLSPLHAIILILVSLFFTIVGGFIPSLIA
                     AKKDPVEALRTE"
BASE COUNT          581 a          413 c          330 g          687 t
ORIGIN      
        1 acttttacaa attgcattta ctacttccat tttgcgttta ctttttcaat taaccattac
       61 atgaaagaat aacgtttttc aggacaaaat acaactgtac ggtaattccg tacaaaggtt
      121 gacttgaaag gtggaactaa aatgagccat aaaaaatgtc agtctatttc ttgtgatgtg
      181 gcaagctgct cttttaacga tacgcttgaa aacagctgta ctttgtcagc tataaaggtt
      241 acgcctaaaa tgaactgcgc aactgaagat attgatgaaa gcatgtgtgc aagctataag
      301 agcagggcac tggtagacag ataaatttct caattaatga ataatttgtc tttttttgag
      361 tataatacta gtgttgccaa ggacgcaaca aaaaattgaa tatggagaaa agaatcggca
      421 ttagagtttg tgccgattct tttattattt attcggttct cagtgcttcc acagggtcct
      481 ttttcgcagc tatcaggctt ggtatgaatc ctccaacaat tgtaaagaac aagcttacaa
      541 gaatcagaat aattgcatgc aaaggcgaaa gctttgctac gttttcaaga tttgaataat
      601 ttgcaattat catgtttgta ggtatgacca gcaaagacgc tatggctatt ccaattaatc
      661 ctgaaaacac ccctataata aaggtttctg cattaaatac caaggtaata tccgattttc
      721 ttgctcctaa tgacctcaga attccaatct cttttgttct ttccagtacg gaaatgtacg
      781 taattattcc aatcattata gacgaaacaa acaaagaaat tgaagcaaaa gctataagga
      841 caatggatat tgaatctatt atctttttcg taagctttgt tatctgctcg gccatgtctg
      901 tatatattat cctgtccttg ctgtctttgc cttcattgta cgcatccaaa aacgccaata
      961 cgtcatcctt tgaatcaaaa tctctaggaa agattgttat cattaaagga acagagtttg
     1021 ctccaattac ccggagaaca ttttccttgt cttcctttcc cttttccgtt gtcaaatcaa
     1081 aaggttggcc tgtaagcaca ttgtaatcag cctccctttg ggcttttgca atgtcgctgt
     1141 tttccgaatc ctccagaata tattccatca gttcttcaga atatgccacg cctgtacgga
     1201 ttacatccag catcttgtct ttctttatcc ttaggattcc tacaattttt aaaggtattg
     1261 ccgtttccct attatatatt tcagtcatgt ccgcagggtt gccaaccagt gtaaagaatc
     1321 catcccgttc cacgtaatag tcattattag gaatggctct taactcggta ccgattatgt
     1381 cctcaaaagc tatactttca taagttgaaa aacctagcgt ttccagcact cttttgtcca
     1441 gtctattgaa tttgtccaca accagcaata actcgttttt gttctctggc atttttccat
     1501 aaagtatgtc atagttcttt tcaacaaaac cttcttcacc aaactttttt ggaagcacac
     1561 tcatatccag gtcttgcatc gatacaatct ttgccacatt gtctctgtca agtttcaata
     1621 agttcaaaga tgtcatgcgt gtatatgtgg ttcccaaaag cagattcgga tcaatgctct
     1681 ttaaatactc tatatattcc tctgtaatat tgttcatatg aattaattca ctttcgctcc
     1741 tgtcaaaagg aattacttcc tttttgtctg taaactcctt gtacttcctg ttctcctctc
     1801 caaacttttt acccatatcg gcaatatcaa cagttgtcgc ctgctgtcct atgattatgg
     1861 ggaatccaga catggtgttt tcctcaaact tgtctatttc tatctgaaac ccgttcgaaa
     1921 gagcgagaat gagtgcaatc cctatgattc caatgcttgc tgcgaaagag gtaagtattg
     1981 ttctaaattt ctttgtcttt atgttgttaa a
//
LOCUS       DAONVY010000437         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37663_length_2007_cov_2.777664, whole genome shotgun sequence.
ACCESSION   DAONVY010000437 DAONVY010000000
VERSION     DAONVY010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37663_length_2007_cov_2.777664"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..>2007)
                     /locus_tag="PLI11_09935"
     CDS             complement(305..>2007)
                     /locus_tag="PLI11_09935"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPZ53228.1"
                     /translation="WVVRSFIPMEFKKYCKVTSDIKLEGHDKAKGEGGWGHITYVKYD
                     TYNVYKREIGVEKLKNMAENHTHDPKYSEKNEYREIKGLTIKAGESVKIFDKKAEGSI
                     ASIKLYLAGENVTHSVLKDLWIRIYWDGHEESDVSAPIGTFFGNQYNLSGCENHHLML
                     GMSLKLGEYLKCYNYFPMPFWERAVVELNNSSMRDIDIHWAEIGFTPSSVCRYDKQKT
                     GYFVSSEYYPKTENVQGKNSVIANVEGSGHMVYGVMSGYGIMNGCEGDVRVFIDGRKS
                     PEIESDGSESWASYGWGFVTPPQANPFSGYNGAYNSNSYWSMVRLMLGDSYYFKQSIR
                     FELEHGGANEGLGCHSGQIFLYMLPESMEAYVVTDEIDVSDKESLDKHDYTVTGSYDT
                     KTVYSAYANGINTDSKEGLIHVDFNGRISFYVNIDKNNRGVILNRTSSQQVGRHCANI
                     YVDDVLVEEAKWYYADNNPYYQCLDDSFQIPEKYTKGKDRIRITIEPVEVDGVKTWNH
                     AKYQVVSLLFREEIAQEELVNPDVKDKFFTLPLLLALGLLVVAGTAVHYIRRKKREGS
                     "
BASE COUNT          546 a          497 c          324 g          640 t
ORIGIN      
        1 gcacctttat ttgcaatgcc aaagattcgt cttttgaaaa atccgcaagt ttggcacata
       61 tttcacctaa gattttcgct ttttcatctt tgtatttaaa gggaatactg cacaaggaca
      121 gaaaatcaaa aaacttgttg taagtatatg agatatgcgc ccagctcaat ttgctccact
      181 ccgtcacctt tatatgatgc ttgacataag gtttgatcca gaaagcatct gtcctttatc
      241 ccatgcttcc ctatttcctc tccatttttt ccaattatcc accgtggttc caatcccatc
      301 gtattcagct tccttctctc ttctttctcc tgatataatg cacagctgtg ccggccacca
      361 ccaaaagtcc caaagcaaga agcaaaggca aagtgaaaaa cttatctttg acatcaggat
      421 taacaagttc ctcctgtgca atttcttctc tgaacaacaa ggatacaacc tgatattttg
      481 catgattcca cgtttttacg ccatccactt caacaggctc aattgttatc ctgattctgt
      541 cctttccctt tgtatacttt tctggaatct gaaaggaatc atcaagacac tggtaatatg
      601 ggttgttgtc tgcatagtac cacttcgcct cttcaaccaa aacatcatcc acatatatat
      661 tggcacagtg tcttccaact tgctgggagg acgtcctgtt taaaataaca cctctattgt
      721 ttttatcaat gtttacataa aaagatatcc tgccgttgaa atcaacatgt atcaaccctt
      781 ctttgctatc cgtgtttata ccgttggcat aggcggaata aacagtttta gtgtcatagc
      841 ttcctgtaac agtataatca tgcttgtcaa gggattcttt gtccgaaaca tcgatttcgt
      901 ccgtaaccac atatgcctcc atgctttcag gcaacatgta taaaaagatt tgtcctgaat
      961 gacagccaag cccctcgttg gcacccccgt gttccagttc aaatctaata gactgtttga
     1021 agtagtatga atctcccagc atgagcctca ccatggacca ataggagttg gagttgtatg
     1081 ctccattgta tccgctgaag ggatttgctt gcggtggtgt tacgaatccc cacccgtagg
     1141 aagcccagct ttcagagccg tcactttcaa tttcgggaga cttgcgtcca tcaatgaata
     1201 ccctaacatc accttcgcac ccattcatga taccgtaccc ggacataaca ccataaacca
     1261 tgtgccccga gccttccaca tttgcaatta cactgttttt tccctgtaca ttctctgttt
     1321 tgggatagta ctcagaggag acaaaatagc cagttttctg tttgtcatac cggcataccg
     1381 aactgggagt aaaaccaatc tcagcccaat gtatatcaat atccctcata ctgctgttat
     1441 tgagctccac caccgccctt tcccaaaaag gcattgggaa gtaattgtaa cacttaagat
     1501 attcccctag tttaagactc attccaagca tgaggtgatg gttttcacaa ccagacaggt
     1561 tgtattgatt gccaaagaaa gttccaattg gagctgacac atcactttcc tcatgtccat
     1621 cccaatatat tctaatccaa agatctttaa gaacactgtg agttacattc tcacctgcaa
     1681 ggtacaattt aatagaagca attgaacctt cagctttttt gtcaaaaatc tttacagact
     1741 cacccgcttt aatggtcaag ccctttattt ccctgtattc atttttctct gaatactttg
     1801 gatcgtgtgt atggttttca gccatgtttt taagcttttc aacacctatc tcccttttat
     1861 atacgttata tgtatcatat ttcacatatg ttatatgacc ccagccacct tcacctttgg
     1921 ctttatcatg cccttcaagc ttgatatccg atgttacctt gcaatacttc ttgaactcca
     1981 taggtataaa gcttctgaca acccaga
//
LOCUS       DAONVY010000438         2006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37687_length_2006_cov_2.832394, whole genome shotgun sequence.
ACCESSION   DAONVY010000438 DAONVY010000000
VERSION     DAONVY010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37687_length_2006_cov_2.832394"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..604)
                     /locus_tag="PLI11_09940"
     CDS             complement(101..604)
                     /locus_tag="PLI11_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53229.1"
                     /translation="MRQLVYLSLIAIAFVCIFNSSVMAEQGMIDESDIESLIRRAFEV
                     YANLYKDPRNVIDFNAEPIKPEYYPGKGDYATAESLERLLNSVFTENMLEMLERDFDA
                     YYKIEEGNVIFRNNYDRKYSYALPESVLDNTQIVYNRENEAYVITTYIQHSRFFTETR
                     PGILVCT"
     gene            965..1582
                     /locus_tag="PLI11_09945"
     CDS             965..1582
                     /locus_tag="PLI11_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HPZ53230.1"
                     /translation="MVINKFDSYDIDILTEIGNIGAGNAATALSEILQGRVDMSVPKV
                     GILKFKELINELNGPENIIVAILVEVSGDLDGFILFALETECALTLLRLVGMEYGKDV
                     FSLSELEMSTLTEITNILTGTYLTAVCDLTGLAINATVPSFAIDMAGAILNVPISFYG
                     RVGDSVLLIETQFKSGDDLVIGRFMLIPDVSSYKTLLESLRNGEK"
     gene            1579..>2006
                     /locus_tag="PLI11_09950"
     CDS             1579..>2006
                     /locus_tag="PLI11_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPZ53231.1"
                     /translation="MTIVSGGIIIVGIGELFATTSPTKLQALGLGSCVGVALYDPVTK
                     VGGLAHVMMPESNGSSNELRGKYADTAVANLLSDMQKLAARKENIVAKLVGGASMFSL
                     SHDVFRIGEKNVIAAKKSLNDLGIRILAEDVGGSVGRTML"
BASE COUNT          569 a          356 c          443 g          638 t
ORIGIN      
        1 tcttccaagg ttattttttc atcccccaga ccagggagta cgctgaagaa ttttggtacg
       61 tagttaacat cgttgatttt ggactcgctt ccatcgttcg ttatgtacag accaatatgc
      121 caggtcttgt ttcagtaaaa aacctggaat gctggatata tgttgtaatt acatatgcct
      181 cattttcccg attgtaaact atttgggtat tatccagaac tgattcaggc agtgcatatg
      241 aatattttct gtcataatta tttcgaaaaa ttacgttgcc ttcctcaatt ttgtaataag
      301 cgtcaaagtc tctttcaagc atttccaaca tgttttccgt gaaaactgag ttcaaaagac
      361 gctccagtga ttcagcggtc gcatagtcac cttttccagg ataatattca ggctttattg
      421 gttctgcatt aaaatcaatc acattccgtg ggtctttgta cagatttgca taaacttcaa
      481 atgcacgtct tattaggctt tcgatgtcgg attcatcaat catgccttgt tctgccataa
      541 cggatgaatt gaaaatacag acaaaagcaa ttgcgatcaa cgataaataa accagttgtc
      601 tcatataatc actccgttct tcgaaataat cattaatatt atttcatatt tatatatatg
      661 atgataccgg ttttgggaat tgataccaac aaatagtttg ttgaattcta aggggtgata
      721 acaaaaagcg tcatgttcaa gtcatgacgc ttgattttgc ttaaaaatgt gttttcaggg
      781 tatctgtatt cttttccacc agtgattata gcttttctgg caagatatgt atatatcgtt
      841 tcttgtctgc tattaaataa aaattatcca acatgaaatt tctgctaaag tatttagttc
      901 agtgttcgat aatagagtaa taagcttgtt tatgcaagct ttagtaatac ttggaggaaa
      961 acaggtggta attaacaaat ttgattcgta tgatattgac atacttactg aaataggaaa
     1021 cattggcgca ggaaacgcag ccaccgcctt atccgagata ctccagggac gggtggatat
     1081 gagtgttccc aaggtgggta ttttaaaatt caaggagctg attaacgagc ttaatggccc
     1141 tgagaatatt attgtggcca ttttggttga agtcagcggt gatttggatg gtttcatcct
     1201 ttttgccttg gaaacggaat gtgccttgac attattaaga ctggtgggca tggagtatgg
     1261 caaggatgtt ttctccttgt cagaacttga gatgtccact ttgacagaga taaccaacat
     1321 acttacgggt acatatctta ccgccgtgtg cgacctgacc ggtcttgcca ttaacgcaac
     1381 agtcccatcc tttgcaattg acatggcagg agcaatactg aatgttccca taagcttcta
     1441 tggacgggtt ggcgattccg ttcttttaat tgaaacccag tttaaaagcg gagacgatct
     1501 tgtcatagga cgcttcatgc tgattcccga tgtcagctca tacaaaacac tgctcgagtc
     1561 attaaggaac ggagaaaaat gactattgta agtggcggta taatcattgt cggaattggt
     1621 gagttgtttg caacaacttc tcctaccaaa ctgcaggctc tgggactggg ctcttgtgtt
     1681 ggtgttgcat tgtacgaccc tgtgactaaa gtgggtggtc ttgctcatgt gatgatgcct
     1741 gaaagcaacg gcagctcaaa cgagcttaga ggaaaatatg cggacacagc tgtggcaaat
     1801 ctattgagcg acatgcaaaa acttgctgca aggaaggaga atattgtggc aaaactggtt
     1861 ggaggagctt caatgttttc gttgtcgcac gatgtattta gaatcgggga gaagaatgta
     1921 attgctgcga aaaagtcttt gaatgatttg gggattagga ttttggcgga agatgtgggt
     1981 ggcagtgttg gtagaacaat gttatt
//
LOCUS       DAONVY010000439         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37966_length_1994_cov_3.577102, whole genome shotgun sequence.
ACCESSION   DAONVY010000439 DAONVY010000000
VERSION     DAONVY010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37966_length_1994_cov_3.577102"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1972
                     /locus_tag="PLI11_09955"
     CDS             257..1972
                     /locus_tag="PLI11_09955"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPZ53232.1"
                     /translation="MLITDREELQQYNTSQRLWQGIPGIEISDKGRLFAVLYSGGTKE
                     EFGNYCALITSDDDGLTWSEPVAVAYNGPASRCYDPCLWMDMLGRLWFIWSIMPEHAV
                     YASLCNNPDADILNWSKPFIIGKDVMMNKPTFLSTGELMFPIAVWDRNVQAVKGCVSE
                     GEERLPFVYRSTDCGTTFERLGGPKVEKRSFDEHMILELSDGRLMMFIRTLYGIGKSY
                     SYDGGRTWTDAEASGYVGPSTRFHIRRLSSGRILLIYHDSTSKRNNLAAYLSDDEGET
                     WKWKLMLDERDNVSYPDAVEAKNGFIYIIYDRERGAFCKGLEELYHNAREILMAKITE
                     EDIMEGKIISEDSRLKQIVSKLGVYLGPMINPYSEKLLLSTDEYVKQVMDLPANEKMI
                     DSILEDFGRCSLTLDWDTIQNLNAKIEYALNLDKKTSRKELEKTIREILFIFKKGEEA
                     NPVDLFPKIISFINNNLCVDMSLDEMAQALHLSKFYMCHLFKEKAKITIMSYRNARRI
                     QLAKKQLATTELSITDIALSLGYTDAAYFSKLFMQYEGMTPTQYRKTSRKINNMGLCK
                     LNMKK"
BASE COUNT          656 a          317 c          456 g          565 t
ORIGIN      
        1 cacaaagcga gaaatgccgt gtatttatat agacagttgt gtccagtata gtcataagac
       61 aatattgagt accgatttgt cggactcatt atatcatata tcggtcagtt ttcactactt
      121 cgtgcgaaaa tcaacttttt gctcctttct aattatcaaa ttctgatggt agagaataca
      181 gcaatatagt tattgttttt acaactattt tcaaatattg tatgatagtc ggcaaccagt
      241 ataaggagtt ggaataatgt taatcacaga tagagaagag ttgcaacagt ataatacttc
      301 gcaaagactg tggcagggaa tcccgggaat agagatttct gataaaggga gactttttgc
      361 tgtcttatac agtggtggaa ctaaggaaga gttcggtaat tattgtgcac taataactag
      421 tgacgatgat ggattgactt ggtcggagcc cgttgctgta gcatacaacg gtcctgccag
      481 ccgatgctat gacccttgtt tgtggatgga tatgctgggt agattgtggt ttatttggag
      541 catcatgcct gaacacgccg tctatgcatc cctttgcaat aatcctgatg cagatatatt
      601 gaattggagc aaacccttta tcataggcaa ggatgtcatg atgaataaac caacatttct
      661 gtctactggt gaattaatgt ttccaattgc tgtttgggat agaaatgtac aagcagttaa
      721 agggtgtgtt agtgaaggag aagaaagact tccttttgtc tacagatcca cggattgcgg
      781 aaccacattt gaaagactgg gtggcccaaa ggttgaaaaa agaagctttg acgaacacat
      841 gattcttgag ttaagtgacg gaaggcttat gatgtttatc agaacgttgt atggtattgg
      901 caagagctat tcctatgatg gcggcaggac ctggacggat gcggaggcaa gtggttatgt
      961 tggtcctagt acacgttttc atattcgaag gctgtcttca ggaagaattc tcttaattta
     1021 tcatgacagc accagtaaaa gaaacaatct tgctgcatac ctttccgatg atgagggaga
     1081 aacttggaaa tggaagctta tgcttgatga aagggacaat gtatcctatc cggatgcagt
     1141 agaagcaaaa aatggtttta tatacatcat ttacgataga gaacggggag cgttttgcaa
     1201 gggtcttgag gagctttatc ataatgcccg agagattctt atggccaaga taactgaaga
     1261 ggatattatg gaaggaaaga ttatcagtga agatagtaga ttaaagcaaa ttgtatcaaa
     1321 gctgggagtt tatcttgggc caatgataaa cccatatagc gagaagctgc ttttatcaac
     1381 ggatgaatat gtaaagcagg tgatggatct tcctgctaat gagaagatga ttgacagcat
     1441 actggaagac ttcggacgct gcagtttgac acttgattgg gatacaatac agaacctgaa
     1501 tgcaaaaatt gaatatgctt tgaacctgga caaaaagacg agcagaaagg aacttgagaa
     1561 gacaatcaga gaaatactat tcatattcaa aaagggagaa gaagctaatc ccgttgattt
     1621 gttccccaaa ataatatcat ttatcaacaa taacctgtgt gttgatatgt cactggacga
     1681 aatggcacag gctcttcatc taagcaagtt ttacatgtgc catttgttca aggaaaaagc
     1741 caagataaca atcatgagtt ataggaatgc aagacgtata cagcttgcaa aaaaacagct
     1801 ggcaacaaca gagttgtcaa ttaccgatat tgcactcagt ttaggttata cggatgctgc
     1861 gtatttttca aagttgttta tgcaatacga gggtatgaca cctacacaat acagaaaaac
     1921 aagtagaaaa ataaataata tggggctttg taaactaaac atgaaaaaat agcacaatgt
     1981 tttatagaat tgct
//
LOCUS       DAONVY010000440         1993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_37983_length_1993_cov_3.617647, whole genome shotgun sequence.
ACCESSION   DAONVY010000440 DAONVY010000000
VERSION     DAONVY010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37983_length_1993_cov_3.617647"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLI11_09960"
     CDS             complement(<1..271)
                     /locus_tag="PLI11_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HPZ53233.1"
                     /translation="MAQKEYGNDSISSLKGADRVRLRPAAILGSDGLEGCQHTFIEIL
                     ANSIDEAREGFGDVIEVTRFKDRSIEVRDYGRGCPVDYNEKEKRYN"
     gene            complement(308..>1993)
                     /locus_tag="PLI11_09965"
     CDS             complement(308..>1993)
                     /locus_tag="PLI11_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPZ53234.1"
                     /translation="PSVETLLHNLFQYKFVVHTHPAMLNGLTCGKDGRKIADELFGDR
                     IIWIDLVEPGYVLATCLNKAMKEYTEKTNKDADIIILQNHGVFIAGDSEEEIDEKTKF
                     IFDALKDRIKEYPDLTEANFDKEKAALIAPAIRMLLRDGGTSFVTFRTNNEILKFMEN
                     EESFYPVSSAYSPDHIVYCRPWPLYVKKADDMEQQYDLIEKGVKEYAGKHGFYPKIVC
                     IQGLGYFAWGTSKKNADICADVFLDTIKIGVYSKAFGGPLFMSDAMIDFICNWEVEAY
                     RSKVSLGSGNAKRLNGKISIVTGSAQGFGQGISEEMLKAGANVVIADLNYPLAKENSD
                     EMNKKYGGKATIAAKVDVSNEDNVRDMVYDTVLEYGGLDIFVSNAGVAIAGGLEEMTV
                     DKFEFVTKINYTAYYLCTKYASRIMKIQNRFDENYYMDIIQINSKSGLTGSNKNFAYA
                     GSKFGGVGLTQSFALELVPYNIKVNAICPGNLLSGPLWSDPEKGLFVQYFKAGKVPGA
                     KSVEDVKRHYENLVPMKRGCEIIDVATAIFYVVEQKYETGQAIPVTGGQNMLN"
BASE COUNT          519 a          478 c          349 g          647 t
ORIGIN      
        1 agttgtatct cttttccttc tcgttgtaat cgacgggaca tccgcgtccg tagtcgcgca
       61 cttcaattga gcgatcctta aaacgagtaa cttcgatgac atctccgaag ccttcacggg
      121 cttcatcgat tgagttggcc aaaatttcta taaaggtgtg ctggcaaccc tcaagtccgt
      181 cggacccgag gattgctgct ggacggagtc ttactctgtc cgctcccttt agagaagaga
      241 tgctgtcatt accgtattct ttttgcgcca ttaatactca tacacccttt ctcttttctt
      301 ttgtatatta attcaacatg ttctgtccac cagtaacagg tattgcctgt cctgtttcat
      361 atttctgttc tacaacatag aatattgctg tggcaacgtc tataatttca caccctcttt
      421 tcatcggaac aagattttca taatgtcttt ttacatcttc aacactcttt gcaccgggca
      481 ccttgcctgc cttgaaatac tgtacaaaca gtcccttttc aggatcagac cacaatggac
      541 cggagagaag attacccggg catatagcat taaccttgat gttgtaaggt acaagctcca
      601 gtgcaaaact ttgagtaagt cctactccgc caaacttgct tccagcatat gcaaagttct
      661 tgttgcttcc ggtaagacct gacttggagt tgatttgaat tatatccatg tagtaattct
      721 cgtcaaacct gttctgaatc ttcattatcc tcgatgcata tttagtacac agataatatg
      781 cggtgtagtt tatcttagtt acaaactcaa acttgtcaac agtcatttct tcaagaccgc
      841 cggcaattgc aacacctgca ttacttacaa atatatcaag gccaccgtac tcaagaacag
      901 tatcgtaaac catgtctctt acattgtcct cattacttac gtcaaccttt gctgcgatgg
      961 ttgccttgcc gccatacttt ttgttcatct catcgctgtt ctcctttgca agaggatagt
     1021 taagatctgc aattacgaca tttgctcctg ctttgagcat ctcttctgaa ataccctgcc
     1081 caaatccctg agcactgcct gtaacgatgg agatttttcc attcaatcgc ttagcatttc
     1141 ctgagccaag gctgaccttt gagcgataag cttccacttc ccaattgcaa ataaaatcaa
     1201 tcatggcatc ggacatgaac aagggtccgc caaaggcttt tgaataaacg cctattttta
     1261 tggtgtcaag gaacacatca gcacaaatgt cggcattctt cttgcttgtt ccccatgcaa
     1321 agtatccaag accctgtatg caaacaatct ttggatagaa tccatgtttt ccagcgtatt
     1381 ctttaacacc cttttctatc agatcatact gctgttccat atcgtccgcc ttctttacat
     1441 aaagtggcca gggtctgcag tatacgatat gatccggtga atatgcggag gatacgggat
     1501 aaaaactttc ctcattctcc ataaacttta aaatctcatt gtttgttctg aaagtaacaa
     1561 aggatgttcc accatctctc agaagcattc tgatagctgg tgcaatcaaa gctgcttttt
     1621 ccttgtcaaa attagcttca gttaaatcag gatattcttt tatcctgtct tttaatgcat
     1681 cgaatatgaa tttggtcttt tcatctattt cttcttcaga gtctcctgca ataaatactc
     1741 cgtgattctg cagaataata atgtcagcat ccttgtttgt cttttcagta tactccttca
     1801 ttgccttgtt aagacaggtt gccagaacgt aaccaggttc aaccagatct atccagatta
     1861 ttctgtctcc aaacaattca tccgcaatct ttctgccgtc ttttccgcaa gtaagaccat
     1921 tcaacatggc tggatgagtg tgtactacga atttgtattg gaaaagattg tgcagtagag
     1981 tctcaacact ggg
//
LOCUS       DAONVY010000441         1984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_38211_length_1984_cov_3.718507, whole genome shotgun sequence.
ACCESSION   DAONVY010000441 DAONVY010000000
VERSION     DAONVY010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38211_length_1984_cov_3.718507"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLI11_09970"
     CDS             <1..335
                     /locus_tag="PLI11_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPZ53235.1"
                     /translation="IFRGGLLGVSKGQYEAAKVLGFSTLQTNVRIVIPQMLRITLPSV
                     ANEFITLVKDTSLITVLAIDEILNLAKKIVNTDVNISAYAVVAVYYLIATFLLTKLFE
                     YLEKKFAY"
     gene            352..1110
                     /locus_tag="PLI11_09975"
     CDS             352..1110
                     /locus_tag="PLI11_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPZ53236.1"
                     /translation="MVEGAVISVRGLSKSFGKTKVLNNINFDVYKGETIAIIGPSGVG
                     KSTMLRCLINLEKADAGSILIDGEYLVRDGHYENERTCRKICSQMGMVFQSFHLFPHL
                     TVLDNLIYAPKYVNREDEASAKKRADEILRKVGLADRKESMPSQLSGGQQQRIAIARA
                     LMMNPEILLFDEPTSSLDPLLKNEVLSVMRQLAEEKMTMLVVTHEMGFAKEVADRVLF
                     VSEDGIIADDSPDKIFNLPSNPIIKEFICSIKAV"
     gene            1228..1578
                     /locus_tag="PLI11_09980"
     CDS             1228..1578
                     /locus_tag="PLI11_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPZ53237.1"
                     /translation="MDNPTCSCFLLHEDIVEKAKKALPDENKLMDLADLFKVFGDSTR
                     IKILYLLSVNEMCVCDIANLIGLSQSAISHQLRVLKQAKLVKYRREGKVVFYSLDDDH
                     VSSIIKQGLEHINE"
     gene            1594..1812
                     /locus_tag="PLI11_09985"
     CDS             1594..1812
                     /locus_tag="PLI11_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPZ53238.1"
                     /translation="MRKTLTLQNLGCASCADKMERRINKIDGVISASISFAAQKLFLE
                     VEEGKFEKIVEKAQQVCRKIEPDCRIVY"
BASE COUNT          680 a          304 c          439 g          561 t
ORIGIN      
        1 aaatattcag aggtggtctg cttggtgtaa gcaaaggcca gtatgaggcg gcaaaagttc
       61 ttgggttcag tacattacag acaaatgtac ggatagttat tccacaaatg ctgagaataa
      121 cactaccctc cgtggcaaat gagtttataa ctcttgtaaa ggatacatca cttatcacag
      181 tactagcaat tgatgaaata cttaacctgg caaaaaagat agtcaacacg gatgtaaaca
      241 tatcagctta tgcggttgtt gcagtttatt acttgattgc cacgtttctg ttaacaaaac
      301 tttttgaata ccttgagaaa aagtttgcat attaggaagg agggatacat tatggttgaa
      361 ggtgcagtta ttagtgtaag aggattgagc aagagttttg gcaaaactaa agttttgaat
      421 aatataaatt ttgatgttta caaaggggaa accatagcca taataggtcc ctcaggtgta
      481 gggaaaagca ctatgctgcg atgtctcatt aaccttgaga aagcagatgc aggaagtatt
      541 ctgattgatg gcgaatatct ggtgagggac ggacattatg aaaatgaaag gacttgcagg
      601 aagatatgct cccaaatggg catggtcttt caaagttttc acctgtttcc acaccttaca
      661 gtacttgata acttaatata tgctccaaag tatgtaaacc gggaagatga ggcatctgcc
      721 aagaaaagag cagacgaaat actcagaaaa gtaggccttg ctgacagaaa ggaatccatg
      781 ccatcccagc tttcaggagg acagcagcag agaattgcta tagcaagagc tttaatgatg
      841 aatccggaga ttctcctatt tgacgaacct acctcatcct tggatcctct tttaaagaac
      901 gaagtattgt ctgtcatgag gcagcttgca gaagagaaga tgacgatgct tgttgtgact
      961 cacgaaatgg gctttgcaaa agaagtggcg gacagagtat tgtttgtttc tgaagatgga
     1021 attattgcag atgattcacc agataaaata tttaacttgc cctctaatcc cataattaaa
     1081 gaattcatat gtagcataaa agccgtgtga aaacacggtc tttttatttc cgtaactatt
     1141 tttccattga cagaatataa ttatggtgta taataatatt acatatgaac aattgttcat
     1201 atgataagat gacatagagg tgctgaaatg gataatccaa catgcagctg ttttctatta
     1261 catgaagaca tagtagagaa agctaaaaag gctttgcctg atgaaaacaa attgatggat
     1321 ttggctgatt tgtttaaagt atttggtgat agtacgagaa taaagatact ttacttgctg
     1381 tcggtaaatg agatgtgcgt gtgtgacatt gcaaacctca taggactaag ccagtctgct
     1441 atttctcacc agcttagggt tttaaagcag gcaaagcttg tgaaatacag aagagaggga
     1501 aaagtagttt tctattctct ggatgacgac cacgtaagtt caataataaa acaaggtttg
     1561 gaacatataa acgaatagag tggaggaaag tttatgagaa agacactgac tctgcaaaat
     1621 ctgggatgtg catcctgtgc agataaaatg gaaagaagaa taaataaaat agatggagtt
     1681 atatctgcaa gcatcagctt tgcggcacaa aagttgtttt tggaagttga agaagggaag
     1741 tttgaaaaaa ttgtagaaaa ggcacaacaa gtatgcagaa agatagaacc tgattgcagg
     1801 atagtatact aacatggcaa agcgtcagaa aattgacatt gcagttataa tggcaactgc
     1861 aataagcctg tttgttcttt tgattgtgcc tgtagacaat tctgcaaaac ttataccgta
     1921 catagccgtt tacctgttga taggttatga aattcttttt aaagcaggta gaaacataat
     1981 taat
//
LOCUS       DAONVY010000442         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_38636_length_1968_cov_4.230528, whole genome shotgun sequence.
ACCESSION   DAONVY010000442 DAONVY010000000
VERSION     DAONVY010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38636_length_1968_cov_4.230528"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLI11_09990"
     CDS             <1..253
                     /locus_tag="PLI11_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53239.1"
                     /translation="KASMECDDADLIKLEVEAGAMILKFACLLGLYKIDGYDVSKETY
                     LKVLKEIQDKTIEMHKRAWLARNKPSRMHEGVEALAKIK"
     gene            339..899
                     /gene="frr"
                     /locus_tag="PLI11_09995"
     CDS             339..899
                     /gene="frr"
                     /locus_tag="PLI11_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPZ53240.1"
                     /translation="MLNKSDYKRFSEKMDKSIAVLEDQLTQIRAGRANPSILNKITVE
                     YYGVPTPINQLGSINVPEARMLVFQPWDVSVLSLVEKAIQKSDIGINPNNDGKVIRIA
                     FPPLTEERRKELTKQVKKMGEDTKIAIRAIRREANEYFKNLEKKSEITEDELKVAEKD
                     VQEMTDQHIEEVDNVVKKKEKEIMEI"
     gene            949..1674
                     /gene="uppS"
                     /locus_tag="PLI11_10000"
     CDS             949..1674
                     /gene="uppS"
                     /locus_tag="PLI11_10000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPZ53241.1"
                     /translation="MLKENDKKSLPMHVAIIPDGNRRWAKAKGYPVKYGHRQGANTFK
                     EIVRYAGELGIKYISFYAFSTENWKRSPSEVESLMELLRGFLKNSDKELGKDKDKIKI
                     MVSGKIDRLSPELQKEIKRIEEETKENTGIIVNICINYGGRDEILECTKQICRKVVEK
                     EISIDKIDEEAFISHLFTKDLPDPDLLIRTSGEVRISNFLLWQLAYTELYFTEKLWPD
                     FGKKDFDAALKEYIERQRRFGAN"
     gene            1688..>1968
                     /locus_tag="PLI11_10005"
     CDS             1688..>1968
                     /locus_tag="PLI11_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53242.1"
                     /translation="MKVKTIAGFITAIVALVIVILGGTTMGVVLLFLNIIGLYEFYHI
                     FELKGHKPIKWVGLLYIPLIPFIFINNISEPLTIKIAGTKDINLFTFIQ"
BASE COUNT          692 a          307 c          464 g          505 t
ORIGIN      
        1 aaaggcctcc atggaatgcg atgatgctga tttgattaag cttgaggttg aagctggagc
       61 catgattctt aagtttgcat gtctgcttgg tttgtacaag attgatggat acgatgtaag
      121 caaggagact tatttgaaag tcttgaaaga gatacaggac aagaccattg agatgcataa
      181 aagagcatgg cttgctagaa acaagccaag ccgcatgcat gaaggtgtgg aggctcttgc
      241 aaaaatcaag tagcgtataa ttatggaaac taatacttta tataaagagg aaagtatggt
      301 aaaatacaaa agagtatcat aaaggaggtt gcacagatat gcttaacaaa agtgattata
      361 aacgattcag tgaaaagatg gacaaatcca tcgctgttct tgaggatcag ttgacgcaga
      421 tacgtgccgg aagagcaaat cccagtattt taaacaaaat aacagttgaa tattatggtg
      481 ttccaacacc tattaatcag ttgggcagca ttaatgttcc tgaagccagg atgcttgttt
      541 tccagccttg ggatgtttcc gtactgagtc tggttgagaa ggctatccag aaatctgata
      601 tcggcataaa cccgaacaac gatggaaaag taatcagaat cgcattccct cctttgactg
      661 aggaacgcag aaaagagctt actaagcagg tcaagaaaat gggagaggat actaagattg
      721 caatcagggc tataagaagg gaagccaacg agtatttcaa aaaccttgaa aagaagtctg
      781 aaataacgga agacgagttg aaagtggcgg aaaaggatgt tcaggagatg acagaccaac
      841 atatcgaaga agtggacaat gtcgtcaaga agaaagaaaa ggaaataatg gagatatagt
      901 tgacaagtcc ccaccttaag tggggattgt gtcagttagg agtgaaatat gttgaaggag
      961 aatgataaga agagcctacc tatgcatgtg gccataattc ccgatggaaa taggagatgg
     1021 gcaaaggcaa aaggctaccc tgtgaaatat gggcatagac aaggggcaaa tacattcaag
     1081 gaaattgtca ggtatgccgg tgagctaggc atcaaatata tttcctttta tgccttttct
     1141 actgagaact ggaaaagaag tccttcagaa gtggaaagtc ttatggaact gttgagagga
     1201 tttctaaaaa attcagataa ggaactgggt aaagacaagg acaagattaa gataatggtt
     1261 tcaggcaaaa ttgacagatt gtcgcctgaa cttcaaaaag aaatcaaaag aatagaagaa
     1321 gaaaccaaag aaaacacggg tattatcgtc aatatctgca taaactatgg tggaagagat
     1381 gaaatattgg aatgcacaaa gcaaatctgt cgaaaagttg tggaaaaaga gattagtatt
     1441 gataaaatag atgaggaggc tttcatttcc catctgttta caaaagacct tcccgacccc
     1501 gacctgctta ttagaaccag tggggaagta aggatttcga actttttgct gtggcagctt
     1561 gcctataccg aactgtattt tactgaaaag ttatggcccg attttggtaa aaaggatttc
     1621 gatgcggctt tgaaggagta tatcgaacga cagaggcgtt ttggagcaaa ttgactggga
     1681 ggtttacatg aaagtaaaaa ctatagcggg ctttataacg gcaatagtcg ctctcgttat
     1741 tgtcattctt ggcggaacaa ccatgggggt agtattgctt tttctaaata taattggatt
     1801 atatgagttc tatcatatat ttgaacttaa gggacataaa ccgataaaat gggttggtct
     1861 tctttacatt ccgctgatac cattcatatt tattaacaac atatccgagc cccttacaat
     1921 aaaaatagca ggtacaaagg acataaatct gtttaccttc atccagta
//
LOCUS       DAONVY010000443         1962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_38793_length_1962_cov_2.115889, whole genome shotgun sequence.
ACCESSION   DAONVY010000443 DAONVY010000000
VERSION     DAONVY010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38793_length_1962_cov_2.115889"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PLI11_10010"
     CDS             complement(<1..994)
                     /locus_tag="PLI11_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPZ53243.1"
                     /translation="MLRENRLTFYTSKEIEPKGWLVSQLKIQAEGLSGNLDKVWPDVR
                     DSRWIGGVREGWERVPYWLDGFIPLAYLLKDEDLIDRAKKYINAIIEGQKEDGWICPC
                     NDNERGRYDLWALFLILKVLVLYHDCSGDERIEDVVYKALANLKRHVAGTTLFNWGMS
                     RWFEALIPIYWMYRRREEEWLLRLARTLYAQGMNYKALYNNWDDMEPRAEWTFQTHVV
                     NAAMAIKSEALMSQALKKEMDADEFAEKMYSLLMEYHGMATMHFTGDECFAGNSPIHG
                     TELCSVAEAMYSYEKIFEVTGKTKWLDRLENLAFNAFPATNSEDMWTHQYVQMTN"
     gene            1095..>1962
                     /locus_tag="PLI11_10015"
     CDS             1095..>1962
                     /locus_tag="PLI11_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPZ53244.1"
                     /translation="MLKINRLVSGGVITNYKCSSKCKHCSYSCSPKWERDYMDETTAS
                     NIFRILKKQGCHSVHIGGGEPFLQPEKLLKVLASAANNGVSVEYIETNASWYIDEDKT
                     MSILYDIKEHGVDTLLISIDPFHNEYIPFWKTKSLIKACYNAGVGVFPWQMEFWPDLD
                     SFDERTTHSLKEYEGKFGKSYIDNLLGRYGINLKGRAFKTFKSQLKMQSFNDIMNSSR
                     PCGLLSGVYHFHLDLYENFIPQSCPGFSIKIDELIKGADQEKYKVLYSLESSGVAGLC
                     NLAVNKWICTKRR"
BASE COUNT          577 a          448 c          350 g          587 t
ORIGIN      
        1 ggttggtcat ctgaacgtat tgatgcgtcc acatgtcttc actgtttgtt gcgggaaaag
       61 cattaaaggc aagattctca agcctgtcca gccattttgt tttgcctgtt acttcaaata
      121 tcttttcata tgaatacatg gcttccgcca cactgcatag ttcagtacca tgaattgggc
      181 tgttgcctgc aaagcattca tcgcctgtaa agtgcatggt tgccatgcca tggtactcca
      241 ttagaagact gtacatcttt tcagcaaact catccgcatc catctccttc ttcaaagcct
      301 gggacataag tgcttctgac ttaattgcca ttgcagcatt taccacatga gtttgaaagg
      361 tccactccgc tcttggctcc atatcatccc agttgttata caacgccttg taattcatac
      421 cctgggcata cagtgtcctt gcaagcctta acagccattc ttcttctctt cttctataca
      481 tccaataaat cggaataagg gcttcaaacc atcttgacat accccagtta aaaagagttg
      541 ttccagccac atgtcttttt aaattggcca acgccttgta taccacatct tcaattcttt
      601 catctccgga gcaatcatgg tataaaacca gaacctttag tattaaaaac aaggcccata
      661 aatcatatct tcctctttca ttatcattgc agggacaaat ccacccatct tctttttgac
      721 cctctataat tgcgtttatg tacttcttcg ccctgtcaat caaatcttcg tccttgagca
      781 aatatgcaag gggtatgaat ccatccaacc agtagggtac tctttcccaa ccttcccgta
      841 ctccaccaat ccacctactg tctcttacgt ccggccagac tttgtccaga tttccagaca
      901 atccttctgc ctgaatttta agctgtgaaa caagccaccc cttcggctca atctcctttg
      961 atgtgtaaaa agtgagtctg ttttctctta acatattcgc accacctgtc tcatctatac
     1021 aatgcgtata ttatatacta ttatcttgtg atatactata ctggtattac aaaataagtg
     1081 aaaaagggat gaatatgctc aagataaaca gacttgtatc cggtggagta ataaccaatt
     1141 acaaatgttc ttcaaagtgc aaacactgtt cctattcctg ttcgccaaaa tgggaaagag
     1201 actacatgga cgaaaccact gcaagcaaca ttttcagaat attgaaaaaa caaggttgcc
     1261 actccgtgca tattggcgga ggagaacctt ttttgcagcc tgaaaagctt ttgaaggttc
     1321 ttgcctcagc tgcaaataat ggagtgtctg tggaatatat tgaaacaaat gcatcctggt
     1381 atatcgatga agacaaaacc atgtccattc tatatgacat taaagaacat ggcgtcgata
     1441 ccctactcat atccatcgat ccattccata atgagtatat acctttttgg aaaacaaaga
     1501 gccttatcaa ggcatgctac aatgcgggag ttggagtttt cccctggcaa atggaattct
     1561 ggcctgattt ggattccttt gatgagagaa caacccactc gttaaaggag tatgagggaa
     1621 agtttggaaa aagttacata gataatctcc tcggaagata cggcataaac ttgaaaggaa
     1681 gagcattcaa aacctttaaa tctcagttga agatgcaaag ctttaatgac attatgaatt
     1741 cttcaagacc ctgcggcctt ctttccggtg tatatcactt ccatttggac ctttatgaaa
     1801 actttattcc ccaatcatgt cctggtttct ctataaagat tgatgaactg ataaaaggag
     1861 cagaccaaga aaagtataag gtgctttaca gtcttgaaag cagtggtgtt gcaggactct
     1921 gcaatctggc agttaacaag tggatatgta ccaaaagaag ag
//
LOCUS       DAONVY010000444         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_38805_length_1961_cov_4.666842, whole genome shotgun sequence.
ACCESSION   DAONVY010000444 DAONVY010000000
VERSION     DAONVY010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38805_length_1961_cov_4.666842"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1231
                     /locus_tag="PLI11_10020"
     CDS             101..1231
                     /locus_tag="PLI11_10020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPZ53245.1"
                     /translation="MTIVLVIDVFDQKKNGTTMSARRFTELLRERGHEVRVISTGQPE
                     KDKYVVKTVRPPIINTLCEKQSFQFAKADKKVIREALKGADVVHLYMAFPLEIRALRE
                     ARKMGIPCTGAFHIQPENITYNIKMRRAEWLAWIIYRLFRRFYNKLDHVHCPSEFIAS
                     QLKRHKYKAKLHVISNGITKEFVPDKEIMEQNKNKNKNRIDILMIGRLSPEKRQDLVI
                     EAAKLSKYSSKIHLHFAGSGPMEKEYKKMASTLHNPATFNFYDQKDLINLIHSCDLYV
                     HTSEIEIEAIACIEAISCGLVPVIANSKKSAAKQFALDERSLFESGNAQDLADKIDYW
                     IENPDEKKRMSEKYVLYSRNYDINKSIDKAEAMFMEAIRDAR"
     gene            1221..1895
                     /locus_tag="PLI11_10025"
     CDS             1221..1895
                     /locus_tag="PLI11_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPZ53246.1"
                     /translation="MHVKKCIHFILYYFIVLPLLYIVNYIVFDLEVEGRENLKGIKGG
                     VVICNHIHTLDCTMIGIAAFPRRATLVSQKENFEIPVAGWFVKHLGSLSTGKGYREKK
                     SFYKKAINTLKNEKLVCIYPEGELEPYCKYIREFKKGAFLIAAKTNSPIIPMRIMMRP
                     CTGLRRIYRRKPLFSIHIEKPIYPKGNTMYEKSQYLYIESLKAMDKIVEQSYTADNLI
                     REERPV"
BASE COUNT          725 a          335 c          406 g          495 t
ORIGIN      
        1 tatcatacat ttacaaaaat atttatttgc agtaatatat ataaaactgt gcttttttta
       61 aaaacagaag aggctttgcc atttatcagg aggaaattaa atgacgatag tacttgttat
      121 tgatgttttt gaccaaaaga aaaatggtac aaccatgtcg gcaaggagat ttaccgagct
      181 gttaagagaa agaggacacg aagtaagagt aatatcgacg ggacaaccag aaaaagacaa
      241 atacgttgtt aaaacagtaa gaccaccaat aatcaacaca ttgtgtgaaa aacaaagttt
      301 tcagtttgca aaagcagata aaaaagttat tagagaagca cttaaaggtg cggatgttgt
      361 tcatctatac atggcttttc ctttggaaat cagagcgtta agagaagcaa ggaagatggg
      421 aataccctgt actggtgcat ttcatattca accagagaat ataacgtaca atattaaaat
      481 gaggagagca gagtggctgg cttggattat ttatcgcctg ttcagacgat tttacaacaa
      541 gctcgatcat gtgcactgcc catcggaatt tattgcaagc caattgaaaa ggcacaaata
      601 caaggcaaaa ctgcatgtaa tatccaacgg aataacaaag gagtttgtgc ctgacaagga
      661 aataatggag caaaacaaga acaagaacaa gaacagaatt gacattttaa tgataggaag
      721 actgtcgccc gagaaaaggc aggacctggt catcgaggcg gcaaaactgt cgaagtattc
      781 ttcaaaaatt catttgcatt ttgccggctc cggcccaatg gaaaaggagt ataaaaagat
      841 ggcctccact ttacataatc ctgccacctt caatttctac gaccagaagg accttatcaa
      901 cctcattcat agctgcgatt tgtatgttca tacctctgaa atcgagatag aagccattgc
      961 atgcatcgaa gcgataagct gcggacttgt gcctgtaatt gcaaattcca aaaaatcagc
     1021 agcaaagcag tttgcactgg acgaaagaag cttgtttgag tcaggcaatg cacaggacct
     1081 ggccgataaa atagattatt ggatagaaaa tcctgatgaa aagaaaagaa tgtcggaaaa
     1141 atatgtactt tattcgagaa attatgatat aaataagagt atagacaaag ctgaagcaat
     1201 gtttatggag gcaataagag atgcacgtta agaaatgtat acacttcatc ctttactatt
     1261 tcatagtctt gccattactc tatatcgtaa actacatagt tttcgatctt gaggtggaag
     1321 gaagggaaaa tcttaagggt ataaaaggcg gggtagttat ttgcaaccac atacatactc
     1381 ttgactgtac gatgatagga attgctgcct ttccaagaag agcaacactg gtatctcaaa
     1441 aagaaaactt tgaaatacct gttgcaggct ggtttgtaaa gcacttagga agtctgtcta
     1501 caggaaaggg atacagggaa aagaagagct tttacaaaaa agcgatcaac actttgaaaa
     1561 acgaaaaact tgtttgtatt tatcctgaag gagagcttga accttactgc aagtacatac
     1621 gcgaattcaa gaaaggtgcc tttttgatag cagcaaaaac aaacagcccg attataccga
     1681 tgcgcatcat gatgaggcct tgcacaggat taagaaggat atacaggaga aaacctttgt
     1741 ttagcataca catagaaaag cctatctatc ccaaaggaaa cactatgtat gaaaaatcac
     1801 aatatctgta tattgaatcc ttaaaagcca tggacaaaat tgttgaacaa agctataccg
     1861 ctgacaatct cataagagaa gaaagacccg tatgattaaa aactatggag ggaacatgca
     1921 atgaaataca acgtggtatt tgtgttctcg gatcagcaca g
//
LOCUS       DAONVY010000445         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_38835_length_1960_cov_3.460892, whole genome shotgun sequence.
ACCESSION   DAONVY010000445 DAONVY010000000
VERSION     DAONVY010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38835_length_1960_cov_3.460892"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLI11_10030"
     CDS             <1..544
                     /locus_tag="PLI11_10030"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797296.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPZ53247.1"
                     /translation="RAVNYTGVGTIEYLLDSEGNYYFMEMNTRLQVEHPVTEFVTGID
                     LVKWQIRTASGVEIDFTNEDARIKGHAIECRINAEKQNNQTGMGNRRVNTLHIPGGAW
                     VRFDTAIYQGYEIPPFYDSMIGKLIVHAKTREEAIRKMHAALCELVIEGVDSNIDKQV
                     EILSDSRFVSGEYSTSFMGC"
     gene            564..1415
                     /gene="accD"
                     /locus_tag="PLI11_10035"
     CDS             564..1415
                     /gene="accD"
                     /locus_tag="PLI11_10035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPZ53248.1"
                     /translation="MLSRNLFKKPNNVLENVGKTSKYVKPQVPEDLWISCANCKTLIL
                     KEELFENNKICPNCSFYYRLAPRERLAMIVDEDSFVEHDKNLVSKNILKFPDYDSKLK
                     KAKDTSNEKEAVVCGVAKIKGMECALFVMNSEFMMGSMGVVVGEKVTRLFEYATQNRL
                     SVVGFTVSGGARMQEGVLSLMQMAKTSCAVKRHNDTGLLYITVLTDPTTGGVTASFAM
                     EGDIILAEPGALIAFAGPRVIEQTIKQKLPEGFQRSEFLLEKGFIDAIVERKALRDTL
                     ANILKLH"
     gene            1432..>1960
                     /locus_tag="PLI11_10040"
     CDS             1432..>1960
                     /locus_tag="PLI11_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPZ53249.1"
                     /translation="MAANTAYDKVLAARSNERPTARKYIEGIFDWFMEFHGDRRYGDD
                     KAVIGGLASLYGFPVTVIGLERGTNIKDRVERNFGCAHPEGYRKALRLMKQAEKFHRP
                     VITFIDTAGAYCGIDAEERGQGQAIAENLYEMATLKTPIIAVLIGEGGSGGALALAVA
                     DEIWMLENSVFSVISP"
BASE COUNT          613 a          328 c          521 g          498 t
ORIGIN      
        1 aagagcggtg aattatacgg gtgttggaac aattgaatat ctgttggact cagaaggcaa
       61 ctactacttc atggagatga acacccgtct gcaggtggaa catcctgtaa ccgagtttgt
      121 tacaggaata gatttggtca aatggcagat cagaaccgca tccggagtag aaatagactt
      181 cacaaatgaa gatgcaagga taaaaggaca tgcaatagaa tgcagaataa atgctgaaaa
      241 acaaaacaat caaacaggta tgggtaacag acgggtcaac actcttcaca tacccggcgg
      301 agcatgggta aggtttgata ctgcaattta tcaaggttac gaaattcctc cattttatga
      361 ttccatgata ggaaaactta ttgtacatgc aaagaccaga gaggaagcaa taaggaagat
      421 gcatgctgca ctgtgtgagc tggtgattga gggtgttgac tcaaatattg acaaacaagt
      481 ggagattttg tccgattcca gatttgtatc cggtgaatat tctaccagct ttatgggatg
      541 ctgatgaaag gaaatgagta aaaatgctaa gtcgcaatct atttaaaaaa cctaataatg
      601 tgcttgagaa tgtgggaaag acatctaaat atgtaaagcc gcaggtgcct gaagatttat
      661 ggataagctg cgcaaattgc aagaccttga ttctcaagga ggaacttttt gaaaacaaca
      721 agatttgccc aaattgttcc ttctattaca ggctggctcc cagggagcgt ttggcaatga
      781 tagtggatga ggattcgttt gtcgaacatg acaagaatct tgtttccaag aacattctta
      841 agtttcccga ttatgacagc aaactaaaga aagcaaaaga taccagcaat gaaaaagagg
      901 cagtggtttg tggtgttgca aagattaaag gtatggaatg tgccctgttt gttatgaact
      961 cagagtttat gatgggatcc atgggtgttg tggttggcga gaaggtaacc agattgtttg
     1021 agtatgctac tcaaaaccgt ctgtctgttg tagggtttac agtatcggga ggtgcaagaa
     1081 tgcaggaagg agtactgtcg cttatgcaga tggcaaaaac cagctgtgcg gttaaaaggc
     1141 acaatgatac aggtcttttg tacatcacgg tgcttaccga cccgactacg ggcggtgtaa
     1201 ctgcaagttt cgccatggaa ggcgacataa ttctagcaga accaggggcg cttattgcat
     1261 ttgccggccc cagggttatt gaacagacaa taaagcaaaa gctgccggaa ggattccaaa
     1321 ggtcggagtt tcttttggag aagggattta ttgatgccat agtggaaaga aaggcattaa
     1381 gggacactct ggcaaacatc cttaaactgc attaaaggag aggaagaaaa aatggctgct
     1441 aatactgcat atgacaaggt ccttgcggca aggtcgaatg aaaggcctac tgcaagaaag
     1501 tatatagaag gaatatttga ctggtttatg gagtttcacg gtgaccgaag atatggcgat
     1561 gacaaggctg tcataggtgg tcttgcttcg ttgtatggtt ttcctgttac agttattggt
     1621 ttggaaagag gaacaaatat taaagatagg gtggagagga actttggatg tgcccatcct
     1681 gaaggatata gaaaagcgct gagacttatg aagcaggctg agaagttcca cagacccgta
     1741 attacattta tagataccgc aggagcgtac tgcggaattg atgctgagga aagaggacag
     1801 gggcaggcta ttgccgagaa cctgtatgag atggcaactc ttaaaacacc aatcattgcg
     1861 gtgcttatag gggaaggggg aagtggcggt gcgctggctc ttgcagtggc ggatgaaatc
     1921 tggatgcttg aaaactccgt gttttctgtc atttcgcctg
//
LOCUS       DAONVY010000446         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_38847_length_1960_cov_2.412598, whole genome shotgun sequence.
ACCESSION   DAONVY010000446 DAONVY010000000
VERSION     DAONVY010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38847_length_1960_cov_2.412598"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..183)
                     /gene="ffs"
                     /locus_tag="PLI11_10045"
     ncRNA           complement(84..183)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLI11_10045"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            351..797
                     /locus_tag="PLI11_10050"
     CDS             351..797
                     /locus_tag="PLI11_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPZ53250.1"
                     /translation="MIPVEKYGDMLYRLSLSMLRNKQDAEDSVQDTFMRYLYKKPKFN
                     DEEHEKAWLLRVCINVCKNKLRARKRHEYVSMEDLGVYDFTYKDFSIFDAVSKLPEKY
                     KAVILLYYVEEYKVDEIAKILNISSSAVKKRLQRAREMLKVYEENI"
     gene            808..>1960
                     /locus_tag="PLI11_10055"
     CDS             808..>1960
                     /locus_tag="PLI11_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53251.1"
                     /translation="MKRIFILICVIATVFGVCVYSNDNAISESEMESLIVESFEFFSN
                     TFLYTDNIIDENASPVTSGADKYYPGIGDYKSLEQLEEKMRSFYTEEMQNLIDKELEK
                     YKKIVVQGDEVYYRKSDEQDVYSLPEKLMENIRVEYNKDGEAYVIVLYLYYDHGFNDI
                     EIYVRHIGVYIRKTEEGVRVCDVNYVPKYFGVMPKENGDEITLDEVRILAQNAYQLSF
                     FLQNGAYNEFLFNLSGLGSEDADIKSEFGFVKVPCRGFQFYDDWVNLVKRVFTEDKIN
                     EVLGKENNKGFIREGDRVYLLYIGAQYIPLKFYPEMCRIISNDAETAQAEIVLEYHHD
                     PMYENIEKVHLVAEFVKTDDGWRLSGGNYYEYLFGGRNPFLENPQTNDIT"
BASE COUNT          623 a          301 c          459 g          577 t
ORIGIN      
        1 agaggtctcc attttctgta aagagctaca tatgccattt tcttaccttc cttcgtgtga
       61 tgaataaagc caaccctact gggtgtaacc ggggaaactt acggcacacg ataggtcacg
      121 cttaccgctg cttccttccg gacctgacgg ggttcacgtt ctagcgttgc gtaagacccg
      181 gctacaccca taagggttgg ccagctgaaa tatattctac tatagttttg gatttaatgc
      241 aatagtagtt ttttcaacag ttgatattgt gtttttttgg taatataatc aaaattgtgt
      301 caccaaatgc ttgcttcatg tgtgtatatg ttgaagggag ttggaagtta ttgattcctg
      361 tcgaaaaata tggagatatg ctctacaggt tatccttgtc catgttgcgc aacaagcagg
      421 atgccgaaga ttcggtgcag gatacattta tgagatacct ttacaaaaag cccaaattca
      481 atgatgagga acacgaaaag gcatggcttc ttcgtgtttg catcaacgta tgcaaaaaca
      541 aactaagagc caggaaaaga catgaatatg tcagtatgga ggatttggga gtatacgact
      601 ttacatacaa ggacttttcc atatttgatg cagtttccaa actcccagaa aagtacaaag
      661 ccgtcattct actttattac gtggaagagt acaaggtgga tgaaatagcc aagatactga
      721 atatttcttc ttctgcggta aagaagagat tgcaaagagc aagagagatg ctgaaagtct
      781 atgaggaaaa catatgaggt gagtgaattg aaaaggattt ttatcttgat atgtgtgatt
      841 gcaactgtgt ttggtgtttg tgtttattcc aatgacaatg caattagtga aagcgaaatg
      901 gaaagcctta tcgtagagtc atttgagttt ttttctaata ctttccttta tactgacaac
      961 attattgatg aaaatgcatc acctgttacc tctggtgctg ataaatacta tcccggaata
     1021 ggggattaca aaagccttga gcaacttgaa gaaaagatga ggtcttttta taccgaagaa
     1081 atgcaaaatt tgattgacaa ggaattggag aaatataaaa agattgttgt tcagggagat
     1141 gaggtgtact accgtaaaag tgatgagcag gatgtgtatt ctctgcccga aaagctgatg
     1201 gagaatatta gagtggaata caacaaggat ggagaagcat acgttattgt tctttatctt
     1261 tactacgatc atggttttaa tgatatagag atatatgtaa ggcatatagg tgtatatatt
     1321 aggaagactg aagaaggtgt gagggtatgc gatgtaaatt atgttccaaa gtattttgga
     1381 gtgatgccca aagaaaatgg tgatgaaata acactggatg aagttaggat ccttgctcaa
     1441 aatgcgtatc agttgtcttt ctttttgcaa aatggagcat ataacgagtt tttgttcaat
     1501 ttgtctggct tgggcagtga agatgcagac ataaaaagcg aatttgggtt tgtaaaagtt
     1561 ccctgcaggg gttttcaatt ctatgatgac tgggtaaacc tggtgaaaag ggtgtttaca
     1621 gaagacaaaa taaatgaagt gcttggaaaa gagaataaca agggctttat tagagaaggt
     1681 gacagagtat acctcctcta tatcggagct cagtatattc ctttgaagtt ttacccggaa
     1741 atgtgtcgaa taatatccaa tgatgcggaa acggcacagg cagagatagt tttggagtac
     1801 catcatgacc caatgtatga gaatattgag aaagtgcatc ttgtggcgga gttcgtaaaa
     1861 actgatgacg ggtggagatt aagtggcggt aactactacg agtacctgtt tggcggaaga
     1921 aatcctttcc ttgaaaaccc gcaaacaaac gatatcacat
//
LOCUS       DAONVY010000447         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39019_length_1953_cov_3.151212, whole genome shotgun sequence.
ACCESSION   DAONVY010000447 DAONVY010000000
VERSION     DAONVY010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39019_length_1953_cov_3.151212"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLI11_10060"
     CDS             <1..962
                     /locus_tag="PLI11_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ53252.1"
                     /translation="TGAIVCPYELTIAAMGHAMDNGVELFLESRVLDISCDNGAFEVE
                     TTKGTIKARYIINAAGLYADKISRMVGDDSFEITPRKGEYLLLDKELCGLVGHTIFKV
                     PTKMGKGVLVSPTVDGNILLGPTSEDIYDKEDTQTTRVGIEKIINSAAECVKNIPFGS
                     VITSFTGLRATTKNGDFLITAPKPNFLNLAGIDSPGLTAAPAIAVHVSNMLKDMGLEL
                     VEKKDYTSTRKIYQVEKSRYNKMVCRCEKVSEGKIIEAIHTNPKAITVDAIKRRTRSG
                     MGRCQGGFCMPSVVSILARELGIDVCEVRKSGGDSRIVVGKKE"
     gene            967..>1953
                     /locus_tag="PLI11_10065"
     CDS             967..>1953
                     /locus_tag="PLI11_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPZ53253.1"
                     /translation="MQTKDLVIIGRGPAGMAAAVSAYGKGIRDLLIIERDESLGGILQ
                     QCIHNGFGLHMFKEELTGPEYAWRYEKMILDLGIPFKLNSMVVDISKDKVITYVNEEE
                     GEVKIKAKAIILAMGCRERSRGALNIPGTRPAGIYTAGTAQKFINMMGLMVGRQVVIL
                     GSGDIGLIMARRMTLEGASVKAVCEIMPYSSGLARNIVQCLDDFNIPLKLGHTVTKIH
                     GKDRLEGVTISKVDENRRPIPGTEEYIECDTLLLSVGLIPENELTKGMGIELDGSTRG
                     AVVDQNRETSLEGVFSCGNVLHVHDLVDYVSEEAYIAGKAAARYIKGERVKNN"
BASE COUNT          618 a          324 c          521 g          490 t
ORIGIN      
        1 aaacaggggc cattgtatgt ccttacgagc ttactattgc agctatggga catgctatgg
       61 acaatggtgt ggagcttttt cttgaaagca gggttcttga catatcttgt gataacggag
      121 catttgaagt agaaaccacc aaaggcacaa taaaggcaag atacattatc aatgctgcag
      181 gactgtatgc tgacaaaata tccaggatgg ttggagatga ttcttttgag ataactccaa
      241 gaaaaggaga gtaccttctt ctcgacaagg agctgtgcgg cctggttggg cacaccatat
      301 tcaaggtgcc cactaaaatg ggaaagggtg tgcttgtaag tcctactgtg gatggcaaca
      361 tccttctggg acccacttca gaggatattt acgacaagga agacacccaa accaccaggg
      421 ttggtattga gaagattatt aatagtgctg cggaatgcgt gaagaacatt ccttttggca
      481 gtgtgataac ttcttttacc gggcttaggg ctacgacaaa aaatggtgat tttttaatta
      541 cagccccaaa acccaatttt cttaatcttg caggaataga ctcacccggt ctcacagcag
      601 cgcctgcaat agcggtgcat gtatcaaaca tgctgaaaga catgggattg gagctggttg
      661 agaaaaagga ctatacaagt acaagaaaaa tataccaggt agaaaagtcc aggtacaata
      721 aaatggtgtg cagatgtgaa aaggttagtg aagggaaaat aattgaagcc atacacacaa
      781 acccaaaagc cattactgtg gatgctataa aaaggagaac aagaagcggt atgggaagat
      841 gccagggagg cttctgcatg ccgagtgttg tatcgatttt ggcaagagag ctcggaattg
      901 atgtttgcga ggttagaaaa tcaggcggag attcaagaat tgttgtgggg aagaaggagt
      961 gaaaacatgc agacgaagga tttggtcata attggtagag gacctgcagg catggctgca
     1021 gcggtgtccg cttatgggaa gggaataagg gatttgctga ttatagagag ggatgaaagc
     1081 cttggaggta ttttgcagca gtgcatacac aacggttttg gtcttcatat gttcaaggag
     1141 gagcttacag ggcccgaata tgcctggaga tatgagaaaa tgatattgga tttgggtatt
     1201 cctttcaaac ttaactccat ggtggtggat atttcaaagg acaaagtgat aacttacgta
     1261 aatgaagaag aaggagaagt aaagataaag gcaaaagcaa taattcttgc catgggatgc
     1321 agggaaagaa gcaggggagc cttgaatatt cccgggacaa gacctgccgg catttacact
     1381 gcgggtacag cccagaagtt cattaatatg atgggactta tggttggcag acaagttgtc
     1441 atattggggt cgggagacat tggccttata atggcaagaa gaatgactct tgaaggagct
     1501 tctgtcaagg cggtttgtga aatcatgccc tattccagcg gtcttgcaag aaatatagtt
     1561 cagtgtcttg atgatttcaa cattccttta aagcttggtc ataccgttac aaaaatacac
     1621 ggcaaagata gacttgaagg agttacaatt tccaaagttg atgaaaacag aaggccaatt
     1681 ccgggtactg aagagtacat agagtgtgac acacttcttt tgtccgtcgg acttatccct
     1741 gaaaatgagc ttacaaaagg aatgggtatt gagctggatg gaagtacaag aggagctgtt
     1801 gtggaccaaa acagggaaac ttctcttgaa ggggtgtttt cctgtggaaa tgttctgcat
     1861 gttcatgacc ttgtggatta tgtctctgaa gaagcatata ttgcagggaa agctgcagca
     1921 agatatataa aaggggagag ggttaagaac aat
//
LOCUS       DAONVY010000448         1951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39069_length_1951_cov_3.260549, whole genome shotgun sequence.
ACCESSION   DAONVY010000448 DAONVY010000000
VERSION     DAONVY010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39069_length_1951_cov_3.260549"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLI11_10070"
     CDS             complement(<1..613)
                     /locus_tag="PLI11_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53254.1"
                     /translation="MKRILTLFLTAVLSACILLSMGGRSIASDMDKIIKVSCTDALGR
                     TLHDVKGFREDRYVGLFYFLWLGQQNQKEVYDITSILRTHSINELVDINSGKFPNDDV
                     YFFNEPLFGYYNSEDPWVIRKHIELFIYAQIDFLVFDITNGIYYDKVWFSVLEILDKY
                     RLEGYKVPQIVFFTNTRSDEAVKHVYQYLYKRDLYKELWFYGPY"
     gene            787..1872
                     /locus_tag="PLI11_10075"
     CDS             787..1872
                     /locus_tag="PLI11_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPZ53255.1"
                     /translation="MILNKELEKHYLPYTESEKLFKTFYELAKGNKPTDSFLDSIDNI
                     DIRSFMQNVVDTGNYHFSKKCDLFFIYKEDITVSKHIRYYPYFMHKHYFYEIIYQLTG
                     TSRHIINNMDISMEEGNLLIIPPSSFHAAGVLDDSIIVNIIVKPNSLDMLLSDLLPEN
                     NIFETFFSQTGIFHSDINYIYIPSTEDKKLQELVEEIVLEDINKKDLSTSQKKILVMH
                     VLCHLLRHYVDTMKVSKNSNRYPTEVKSICQYLHVNYRNASLTDVAKEFGYSPTYLSK
                     LIHSSTGKTFSEIVCGEKLMAACKLLATTSMKISDIAEHVGYSSPEYFNKVFKMNMGV
                     SPSQYRKEYDTYPAKLYKHHYHIHNEV"
BASE COUNT          675 a          405 c          312 g          559 t
ORIGIN      
        1 catatggacc atagaaccaa agttctttgt ataagtccct tttatacaaa tactgataca
       61 catgttttac cgcctcatca cttctggtat ttgtaaaaaa gacaatttga ggaaccttgt
      121 atccttcaag cctgtatttg tcaaggattt caagcacgct gaaccatacc ttgtcgtaat
      181 atattccatt ggttatatca aatacaagga aatcaatctg ggcatatatg aacaactcta
      241 tatgctttct tataacccaa gggtcttccg agttgtagta gccaaacagc ggctcgttga
      301 aaaaataaac atcgtcattt ggaaacttgc cactgttaat gtccacaagt tcgttaatag
      361 agtgagttct tagaatacta gtgatgtcgt aaacttcctt ttgattctgc tgtccaagcc
      421 ataagaagta gaatagccca acatacctgt cttcacgaaa acctttaaca tcatgcaaag
      481 tccgacctag ggcatccgtg catgaaacct tgattatctt gtccatatca cttgctatgc
      541 ttcttcctcc catacttaat aatatgcatg cagaaagcac tgcagtaagg aataaagtta
      601 aaattctttt cattctatca acctcttttc cacttgccaa acccattata tcttccactc
      661 ctcacaaaaa acaggcaata ttatgtcata tgccatggtt ttctgtaata atttatcatt
      721 caatcaagtt gtgcagtcct gccactaatg atataataaa aatacaaatt gcaggaagtg
      781 aaatatatga tactgaacaa agagctggaa aaacattatc ttccctatac ggaaagtgaa
      841 aagctgttca agacctttta cgaacttgca aagggcaaca aacccactga ctcatttttg
      901 gattccatag ataatattga cataagatcc tttatgcaga atgtggttga tacaggcaat
      961 taccacttca gcaaaaagtg cgatctgttt ttcatataca aggaagacat aactgtttca
     1021 aaacacataa gatattaccc atatttcatg cacaaacatt atttctatga gataatctac
     1081 cagcttacag gtacaagccg tcatataatc aacaatatgg atataagtat ggaggaaggc
     1141 aaccttttaa tcatacctcc aagttccttt catgcagcag gagtgctgga cgacagcata
     1201 atcgtaaata ttatagtaaa gccaaattct cttgacatgc ttctttccga tttgctccca
     1261 gaaaacaaca tattcgaaac ctttttcagt cagacaggga tatttcactc cgatataaat
     1321 tacatataca tcccttcaac ggaagataaa aagcttcagg aacttgtcga agagattgtc
     1381 ttggaagata tcaataaaaa ggatttgtcg acttcacaaa agaaaatact agtaatgcat
     1441 gtactttgcc atctgctaag acattatgtg gataccatga aagtttccaa aaactccaat
     1501 cgttatccta cagaagtaaa aagcatatgc cagtacctgc atgtaaatta caggaatgca
     1561 tctcttactg atgtggcaaa ggaattcgga tactcaccta catacctgag caaactgata
     1621 cattcctcta caggcaagac ttttagtgaa attgtatgtg gcgaaaaact tatggccgca
     1681 tgcaaactgc tggcaaccac atccatgaaa atatccgata ttgcggaaca tgtaggttac
     1741 tccagccctg aatacttcaa caaggtgttt aagatgaaca tgggagtttc accctcccaa
     1801 tacagaaagg aatacgacac atatcctgcc aaactgtaca aacatcatta ccatattcac
     1861 aacgaggtgt gacaatgata agaaacgatg ccttaaaaaa atacatagaa cctttttcga
     1921 acatagagaa gatatacaag aatttgtaca t
//
LOCUS       DAONVY010000449         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39105_length_1950_cov_2.229024, whole genome shotgun sequence.
ACCESSION   DAONVY010000449 DAONVY010000000
VERSION     DAONVY010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39105_length_1950_cov_2.229024"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="PLI11_10080"
     CDS             <1..1448
                     /locus_tag="PLI11_10080"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53256.1"
                     /translation="DVCLEDVAQEFQFEPVKVSEDYEGSDKLFANDIVVYSLDSERTG
                     FIAAGFICKEDADLYIMFDERINEHNDVEGLANGCTNVIKIEMEKGSYDFISFESYGF
                     KYIKFVCTKGEVEVRNVKVREYICSVPLKNLEEIIDKEILQDEKLGKVLEAATNTFKQ
                     NAVDIFMDCPTRERAGWLCDSFFTARAEKVFTGENAIEYNFLENFLLPEKFEHLPKGM
                     LPMCYPSDHYDKVFIPNWAMWFVKELYDFVKRNEDNENAKELAEKLKDRVYELLDYFK
                     KFENEYGLLEKLESWVFVEWSRANDFVQDVNYPSNMLYSSTLENAGRLYNDDKLIEKA
                     EKIKEVILERSFNGEFFVDNEIRKDGVLVSTNETSETCQYYAFFMDIATPDTHKALWG
                     KLVDEMGPKRDASKTYPNVYPSNAFIGAVLRLDLLKRFGYTDKLKEEIKDYFYYMAEK
                     TGTLWENLTPEASCNHGFTSYVAYLLSNKKY"
     gene            1468..>1950
                     /locus_tag="PLI11_10085"
     CDS             1468..>1950
                     /locus_tag="PLI11_10085"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPZ53257.1"
                     /translation="MSRCVIITAYNDYLIKESIEIEKGDFIICADAGYELARKENIVP
                     RVIVGDFDSLDSEVSISGHSLLDVVCLPVEKDDTDTLFCLKYGLEKGFEEFVIVGGFG
                     GRSDHTYANIQTLAYGCDKGVSVRMQDKKNMFTIISNAEIKIPKRENATLSVFAYSDV
                     C"
BASE COUNT          619 a          332 c          488 g          511 t
ORIGIN      
        1 ttgatgtttg ccttgaggat gttgcccagg agtttcagtt tgaaccagtc aaggtgtcag
       61 aagactacga aggtagcgac aagctatttg caaatgacat tgttgtgtac agtctggaca
      121 gtgagcgtac cggctttata gctgcaggtt ttatttgtaa agaagatgcg gacctgtaca
      181 taatgttcga tgagagaatc aacgagcata atgatgtgga aggacttgcc aacggctgta
      241 cgaatgttat caagattgaa atggaaaagg gcagctacga ctttatatcc tttgagtcat
      301 atgggttcaa gtatataaag tttgtgtgca caaaaggaga ggttgaagta cgcaatgtaa
      361 aagtaaggga atacatctgc tccgtgcctc ttaagaacct ggaagagatt attgacaagg
      421 aaatcctgca ggatgaaaaa cttggcaagg tgctggaagc agcaacaaac acattcaaac
      481 agaatgcggt tgacatattc atggactgcc ccacaagaga aagggcgggc tggctgtgcg
      541 acagcttttt tacagcaaga gctgaaaaag tctttacggg agaaaatgca attgagtata
      601 acttcttgga gaacttcctt ttgccagaga aatttgaaca ccttccaaaa ggaatgcttc
      661 ccatgtgcta tccttccgac cattacgaca aggtcttcat acctaactgg gcaatgtggt
      721 ttgttaagga gctttatgac tttgtaaaga gaaatgaaga caatgaaaat gcaaaagagc
      781 tggcagaaaa gttaaaggat agagtatatg aactgctgga ttacttcaaa aagtttgaga
      841 atgaatacgg ccttcttgag aagcttgaga gctgggtctt tgtagagtgg tcccgggcaa
      901 acgactttgt gcaggatgtc aactatcctt caaacatgct ttattcatca actcttgaaa
      961 atgctggcag gctgtacaat gacgacaagt taattgaaaa ggcagagaaa ataaaagagg
     1021 tgattctgga gaggtccttt aacggagagt tctttgtgga taatgaaata aggaaggatg
     1081 gcgtgcttgt ctcaacaaac gaaaccagtg aaacatgcca gtactatgca ttctttatgg
     1141 atattgcgac tccagatacc cataaagctc tttggggcaa gttggttgat gaaatgggac
     1201 cgaagcggga tgcaagtaaa acatatccta acgtatatcc ttcgaatgcc tttatcggtg
     1261 ctgtactgag actggatttg ttaaagcgat ttggttatac tgacaaatta aaggaggaaa
     1321 tcaaggacta cttctattac atggcggaaa aaacgggaac cctttgggag aacctgacac
     1381 ctgaagcaag ctgcaaccat ggatttacat cctatgttgc ttacctgttg agtaataaaa
     1441 aatattaaaa gtgtggggta gatgacgatg tctagatgtg taattatcac agcatacaac
     1501 gattacttga ttaaagaaag tattgaaatt gaaaaagggg acttcattat ctgtgcggat
     1561 gcagggtatg aactagccag aaaggaaaac atcgttccca gagtaattgt tggagatttt
     1621 gattcactgg attcggaagt cagcatttcg ggacacagcc ttttggacgt ggtttgcctt
     1681 cccgtggaga aggacgacac cgataccctg ttttgcctga aatatgggct ggagaaaggg
     1741 tttgaggagt ttgttatagt aggagggttt ggcggtcgaa gcgaccatac atatgcaaac
     1801 atacagaccc ttgcatatgg ctgcgacaag ggtgtaagtg taagaatgca ggacaagaaa
     1861 aacatgttta ccatcatatc caatgcggag attaaaatac ccaaaaggga aaacgccacc
     1921 ctttccgtgt ttgcctattc ggatgtatgt
//
LOCUS       DAONVY010000450         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39145_length_1948_cov_3.038035, whole genome shotgun sequence.
ACCESSION   DAONVY010000450 DAONVY010000000
VERSION     DAONVY010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39145_length_1948_cov_3.038035"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLI11_10090"
     CDS             complement(<1..701)
                     /locus_tag="PLI11_10090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPZ53258.1"
                     /translation="MKNRIRLAIFLIAITFFPIPAANARQTPSFYARNAILIEIDSGR
                     VLYEKQAHVRIPMASTTKIMTALLVLEKANLDDIATVSKRAARIGGSTMNLELGEQIS
                     VLALMYGLMLRSGNDAAIALAEHIAGSVEEFCELMNQKARELGAYNTSFVSPHGLDHH
                     NHYTTAADLALITKEAMKNDLFRKIIATKTITIDGHTMSNTNNLLYANNGIDGVKTGY
                     TSKAGRCIVITAERNG"
     gene            853..1035
                     /locus_tag="PLI11_10095"
     CDS             853..1035
                     /locus_tag="PLI11_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53259.1"
                     /translation="MTVEYSISVCVVLFIAAFCVCFMLRFHNFAIRSANEIEKGPTKA
                     VRVIRATDAVMDAVEE"
     gene            1043..>1948
                     /locus_tag="PLI11_10100"
     CDS             1043..>1948
                     /locus_tag="PLI11_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53260.1"
                     /translation="MKGSISVFVCLILAAVILLNCVLIDTAKVYSLKSGLSARVALSG
                     ESLLAGYDSILREHYNLFALNGGKNLKEEFLTYFNVRNMGYNIGGYFINTPKGYLPMD
                     IHNYNVDNVVVEVCDNLADPSVLEKQIYEEMKIRTPLNLGKSILGKLRILSRTDNMSE
                     SYLKEAEITSLMNEVTEKIEELKRQVEGVYPGDVACVNGYDKHKRHLSYVLLVSSFVN
                     VIKSESPDTKEFEKAITGMKDALLKIKGQASIYLEYNVNAIAKVNEIEKVQKRARNEI
                     KELETWLEKHSPEDEFEVYYKQVITE"
BASE COUNT          561 a          322 c          448 g          617 t
ORIGIN      
        1 ccgtttcttt ctgcagtaat tactatgcag cgccctgctt tggacgtata gcctgttttt
       61 actccatcaa tgccattgtt ggcgtaaagc aaattgtttg tgttggacat ggtatgcccg
      121 tctatggtta ttgtttttgt cgcaattatt tttctgaaca gatcgttttt catggcttcc
      181 ttggtaataa gggcaaggtc tgctgctgtt gtgtaatggt tgtgatggtc aaggccgtga
      241 ggagatacaa aacttgtgtt gtatgctcct aattctcttg ctttttggtt catcaattcg
      301 cagaactctt caacactacc tgctatgtgt tcggcaaggg caattgcagc atcatttcct
      361 gatctaagca taagtccgta cataagggca agtacgctta tctgttctcc taattcaagg
      421 ttcattgtag aacctcctat ccgggctgct ctcttgctta ctgtggcgat gtcgtcaaga
      481 ttagcctttt caagaaccaa cagcgccgtc attattttgg ttgtcgatgc cataggtatt
      541 ctgacatgcg cctgcttttc atacagaacc ctgccggagt caatttcaat aagaattgcg
      601 tttcgggcat aaaaggatgg tgtttgtctt gcgttggcag ctggaatggg aaagaaagta
      661 attgcaatta ggaatattgc tagtcttatt ctgtttttca tttacaaacc tccatttgct
      721 tgtcttttaa tatatatggc aatgattcaa aaatatttca gtaattttct tcggaaaagt
      781 cgaaattcgt cgactggaat taatggatat aaaaaatgct tctatttaga atttgtaaag
      841 gaggctgatt ttatgacagt tgaatattcc atttcagtat gtgtggtttt gtttattgct
      901 gcgttttgcg tttgttttat gcttcgattt cataattttg ctattagaag tgcaaatgaa
      961 attgagaagg gtccgacaaa agcagtcagg gtgattcgtg caactgatgc agtgatggat
     1021 gcagtggaag agtagggata ctatgaaagg gagcatttca gtatttgttt gtctgattct
     1081 agccgccgtc attctgctta actgcgttct gattgatacg gcgaaggtgt attcattaaa
     1141 atcaggactt tcagcaagag ttgccttgtc aggagaatct ctgctggcag gttatgattc
     1201 tatattaaga gagcattaca acctctttgc tttgaatgga ggaaagaatc tcaaagagga
     1261 gtttcttacg tatttcaacg tgagaaacat gggatacaac ataggggggt actttattaa
     1321 tactccgaaa ggatacctgc cgatggatat acataactac aatgtggata atgttgttgt
     1381 ggaggtttgc gacaacttgg cagatccttc tgttttggaa aagcagatat atgaggaaat
     1441 gaagatacgg acgcctttga atcttggcaa aagcatactt ggcaagctaa ggattttaag
     1501 tcgaacggat aacatgtcgg aaagctattt gaaggaagcc gagataactt cactaatgaa
     1561 tgaagtgact gaaaagatag aagaacttaa aaggcaggtg gagggagtat atcctggaga
     1621 tgttgcctgt gtgaatggtt atgacaagca caaaagacat ctgtcatatg ttttactcgt
     1681 atcatctttt gttaatgtaa tcaaatctga aagtcccgat acaaaagagt ttgaaaaagc
     1741 cataaccggg atgaaagacg cccttctaaa gattaaggga caggcctcca tttatcttga
     1801 atacaatgtt aacgcaatag ccaaggttaa tgaaattgaa aaggtgcaaa agcgtgcaag
     1861 gaatgagata aaggaacttg aaacatggct tgaaaaacac agtccagaag atgagtttga
     1921 agtatattac aagcaggtta ttactgag
//
LOCUS       DAONVY010000451         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39171_length_1947_cov_3.054440, whole genome shotgun sequence.
ACCESSION   DAONVY010000451 DAONVY010000000
VERSION     DAONVY010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39171_length_1947_cov_3.054440"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..905
                     /gene="pdaA"
                     /locus_tag="PLI11_10105"
     CDS             66..905
                     /gene="pdaA"
                     /locus_tag="PLI11_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HPZ53261.1"
                     /translation="MRIIAPVILLLTILSFPLILHFNRTKKEYAVYVNEIETGNSIMV
                     NGNMVYRWGLKRNKDGKPPEVGTKEVTMLHKYGGKYIGDTSEKKIYLTFDEGYEQGYT
                     EKILNVLRDNDVKAVFFVTGPYLDKNPELIRRMVEEGHEVGNHTVNHYSLPTKSATIV
                     EKEILELDRVFYEKYGKHMRMLRPPRGEYDEKTLKIAKDLNHTCILWSFAYRDWLVDK
                     QKGADHAYKMIMDHVHNGAVLLLHAVSKDNADALDRVIKDIKQQGYTFGTPDELMNVS
                     VGR"
     gene            912..1157
                     /locus_tag="PLI11_10110"
     CDS             912..1157
                     /locus_tag="PLI11_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HPZ53262.1"
                     /translation="MKSEIFGFPSDVITDTPSIRWVGKNTAWVINHKGLKECDTNSVL
                     INTKLGLVLIQGAELYIKEINAECLRIEGRIASISYV"
     gene            1169..>1947
                     /locus_tag="PLI11_10115"
     CDS             1169..>1947
                     /locus_tag="PLI11_10115"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HPZ53263.1"
                     /translation="MWYNILSYVIISVSGLKPERFVNICARRNIKLWGIKRIDKDTIV
                     MCVSSTDFRKRIRPVAFKARCKVRILEKKGLIIKLYRYRKRKVLAASFAVGIILTMLL
                     CSMLWSIRIEGATPIEEMRTRLVLSSMGIRPGVFMHNIDNSELADTLMDNQPGIAWVG
                     VRKRGIVLEITLAKRQEYNDKGTVPDDVHCDVVAKKDAVVSKIVPEQGTALVTIDTTV
                     TKGQKLIAGWVVQMDEEGKEHKRPVHARGIVKGIVWHSVEIP"
BASE COUNT          633 a          322 c          500 g          492 t
ORIGIN      
        1 ttgcctcctt tttgcatatc tttacagaga catgtatgca aaagggaggt ttttttgtgc
       61 ataaaatgag gattattgca cctgtaatac tgcttcttac catactttcc tttcctttga
      121 tactacattt caacaggaca aaaaaagaat acgcagtgta cgtaaacgag atagagactg
      181 gaaacagtat tatggtaaat ggaaatatgg tgtacagatg gggactcaag cggaacaagg
      241 acggaaaacc gccggaggtc ggtacaaagg aagttacaat gttgcataaa tatgggggca
      301 aatacatagg agacacatcg gaaaaaaaga tataccttac ctttgacgaa ggatatgagc
      361 aagggtatac cgagaagata ttgaatgttc ttagggacaa tgatgtaaag gcggtgtttt
      421 tcgttacagg accctacctg gataaaaacc cggaacttat ccgcaggatg gtggaggaag
      481 gtcacgaggt gggaaaccac acagtcaatc attattctct gcctaccaag tcagccacta
      541 tagtcgaaaa ggaaatactg gagctggacc gggtgtttta tgagaaatat ggaaagcata
      601 tgaggatgtt gagaccaccc aggggtgaat atgatgaaaa gacccttaag attgccaaag
      661 atttgaacca tacctgcata ctatggagtt ttgcgtatcg ggattggctt gttgacaaac
      721 agaagggagc ggatcatgcc tacaagatga taatggatca tgttcataat ggggcggttc
      781 tgcttcttca tgccgtatca aaggacaatg cagatgctct tgacagggtt atcaaggata
      841 taaagcagca gggatacacg tttggaaccc ctgatgagct gatgaacgtg tccgtgggga
      901 ggtagctttt agtgaagagt gagatttttg gttttccttc agatgtaatt acagatactc
      961 cttcaattcg ctgggttgga aaaaacacag cctgggtaat aaatcacaaa ggcctgaagg
     1021 agtgcgatac aaatagtgtt ttaattaata caaagcttgg gcttgtgttg attcaaggtg
     1081 cggaactcta tattaaagaa atcaatgcgg aatgtcttcg catagaaggc agaattgcca
     1141 gtatatcata cgtttaagag gaaagattat gtggtataat attctatctt atgttattat
     1201 atcagtatca ggtttgaagc ctgagagatt tgtaaacatt tgtgccagaa ggaatataaa
     1261 gctgtgggga atcaaaagaa ttgacaaaga taccattgta atgtgtgtca gttcaactga
     1321 tttcaggaaa aggataagac ctgtagcttt taaggcaagg tgcaaagtca ggattcttga
     1381 gaaaaaaggc ctgattatta aactttacag gtatagaaaa agaaaagtac tggccgcttc
     1441 atttgccgtt ggcattatat tgacaatgtt gctttgctcc atgctatgga gtataaggat
     1501 tgagggagct acccccattg aggaaatgcg cacaaggctt gtcctatcca gcatgggaat
     1561 aagacctggt gtgttcatgc ataatataga caacagcgag ctggcagaca cgcttatgga
     1621 caaccagccg gggattgcct gggtgggggt aagaaaaagg ggaatcgtgc ttgaaataac
     1681 gctggcaaaa aggcaggagt acaatgacaa aggtacggtt ccggatgatg tgcattgtga
     1741 cgtggtggca aaaaaagatg cagtagtaag caagattgta cccgaacaag gcaccgctct
     1801 cgttacaata gacaccaccg tcacaaaagg acagaaactg attgcaggat gggttgtgca
     1861 gatggatgag gaaggaaagg agcacaaaag acccgttcat gcaagaggaa tagtaaaggg
     1921 tattgtctgg cattccgttg aaatacc
//
LOCUS       DAONVY010000452         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39238_length_1944_cov_4.256220, whole genome shotgun sequence.
ACCESSION   DAONVY010000452 DAONVY010000000
VERSION     DAONVY010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39238_length_1944_cov_4.256220"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1624
                     /locus_tag="PLI11_10120"
     CDS             62..1624
                     /locus_tag="PLI11_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ53264.1"
                     /translation="MKKNWIRIIAICLLVVFVGTTVGCTIFEEDGPRRTPSSTPKPTP
                     REFVGQITFSTYKRSPNDVSAHAFAHDFAMIYPNVDIVIDEMESAEEYFATLDERIKN
                     GTIGDVFVIDDSRIAEYVENDYIVDFTPYLEDLIEYKTYKRLKPHEILFDAAYKSAQY
                     KKGMYMMPTDYNHKFVFINYDLFSKAGLSVPGDKWTFEDFVRCAEELSKLDEVKIPAV
                     MDYLDPAIWGAFARGFTGVLFPEEEEEEKSTETPASTDENGDATPDPEASPSETPAPT
                     KKEPKIEKPEGSLYYINKEDSIITLTLTNEEVLMGLTELAEIIKQGYVQKADVNPEDL
                     SGIGMIVTDKMEFNTWTDVLRNAEFEWDLIHFPTLPKRFVGVNTLGLAVSTKTAEASE
                     EQKDMSVQFALHTLIQEAAVVYAGNGESVPASKAVNAMKFWREYPVRGKNTSVFATYT
                     ENDFPSIMTYLMNIEAAREYHKIGEAIENYINNGASLEDALKEIEDRVHELWEQEPVY
                     AETPEPTNTPKP"
BASE COUNT          614 a          336 c          456 g          538 t
ORIGIN      
        1 gtcagaagaa actcgtagca ggtattgaac gtattggtat catcgaataa ggagtgtcga
       61 tatgaaaaag aattggatta gaattatagc tatatgtttg ctagtggtgt ttgtcgggac
      121 tacggttgga tgtacaattt ttgaagaaga cgggcctaga aggaccccgt cttcaactcc
      181 aaagccaaca ccaagagaat ttgtaggtca gataaccttc tcgacctaca agaggtcccc
      241 taacgatgtg tcggcgcatg cctttgcgca tgattttgca atgatttatc ctaatgtgga
      301 cattgtaatc gatgagatgg aaagtgctga agaatacttt gccactttgg atgaaagaat
      361 aaagaacggc accattggtg atgtgtttgt aatagatgat tcaaggattg ctgagtatgt
      421 ggagaatgac tatatagttg attttactcc ataccttgaa gatctgattg agtacaagac
      481 ttacaaaagg cttaagcctc acgagatttt gtttgatgct gcatacaagt ctgctcagta
      541 taagaagggc atgtacatga tgcctactga ttacaaccat aagtttgtat tcataaacta
      601 tgatttgttc agcaaagcag gactcagcgt acctggtgac aaatggacat ttgaagactt
      661 tgttcgctgt gcagaagagc taagcaagct tgacgaagta aagattcctg cggttatgga
      721 ttatttggat ccggctatct ggggagcttt tgcaagagga tttacaggag tattgttccc
      781 ggaagaggag gaagaggaaa agtcaacaga aactcctgct tccactgatg aaaatggaga
      841 tgctactcct gatccggagg catcaccaag tgagacgcct gctccaacta aaaaagaacc
      901 caaaatagag aagccggaag gcagtctgta ttatattaat aaagaggata gtataataac
      961 tcttactctt acaaatgagg aagtactgat gggtctcaca gagcttgcag aaataatcaa
     1021 gcaaggctat gtgcagaagg ctgatgtgaa tcctgaagat ttgtctggaa taggtatgat
     1081 agtaactgac aagatggagt tcaacacctg gaccgatgta ttgagaaatg ctgaatttga
     1141 gtgggacctt atccatttcc caacactacc aaagagattt gttggcgtca atactttggg
     1201 tcttgcagtc agcacaaaga cagctgaagc aagtgaggag cagaaggaca tgagtgttca
     1261 gtttgcactt catacgctga ttcaggaagc tgctgttgtc tacgcaggaa acggtgaatc
     1321 cgttccagca agcaaagctg taaatgcaat gaagttttgg cgtgaatatc ctgtaagagg
     1381 aaagaacaca agtgtgtttg caacttacac tgaaaatgat tttccatcca ttatgactta
     1441 tcttatgaat attgaagctg ctcgcgaata tcacaagatc ggagaagcta ttgagaatta
     1501 tattaataat ggagcatcat tagaggatgc gctgaaggag attgaagaca gggttcatga
     1561 actttgggag caagagcctg tttatgcaga aactcccgaa cctaccaaca ctccgaagcc
     1621 ataacggata ctccaatcat agatgtcgat actgcagaca gaaaaaccaa atggtttttc
     1681 tgtttgcttt tgagaatagc ctgtattaca gaataaaacc ttattgtgaa ttatattaat
     1741 attgtgtaac actttgtttt agccacgtgt gtgcaccttg actttagcaa cccgtgtggt
     1801 attataaaat cgtaattaat aaatttggag gtaattatat catgaaacat gcattaaaaa
     1861 ctggtgctgt agttttcttg acactggcca tgttggtcgg ctttgttgca tgtaacagaa
     1921 atcctgagca atcaggcgac gtac
//
LOCUS       DAONVY010000453         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39698_length_1928_cov_2.321410, whole genome shotgun sequence.
ACCESSION   DAONVY010000453 DAONVY010000000
VERSION     DAONVY010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39698_length_1928_cov_2.321410"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLI11_10125"
     CDS             <1..611
                     /locus_tag="PLI11_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53265.1"
                     /translation="DDMADRISLLESKLADLVANGLKYAQVSPVVAVEEKVEKVEEKK
                     EENIVEPKEEPVKEEIVEARPTKESKSAKPIDTLDWNDFLQNITDKNKSSISALIRVN
                     SKGFVLEPNILIIAFSNKMMMDMVVRNNVFDILDESASMAFGKPMKVKLLMEDELEAF
                     SASSAVEENEQENKEKDDNFENALNLLSQLAEDEGFEVFNIE"
     gene            624..>1928
                     /locus_tag="PLI11_10130"
     CDS             624..>1928
                     /locus_tag="PLI11_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53266.1"
                     /translation="MFDIFPISSLERVFMDLPFEAKPLHKATMLKNEYYSFQFAYKLS
                     HMAPATNIPVQLEVTHDFGDSLIVKFVDYVPCDLAIYDNATEPLEHPWPGLFPDILRP
                     MPKQLWAFKKNYRSVWITINGKNIESGTYTITATISSGDTKKSTSFELTVLDKELPRY
                     DFKCTNWFYTDCLCNYYNIEFNSEEYWRITEEFAKEAARHGVNMLLTPIFTPPLDTRV
                     GGERRTIQLVDVYKNGDNYTFNFDKLARWVNMCKRANIEYYEISHLFTQWGCKHAPKI
                     MGYDNGEYKKLFGWETDAHGSEYRSFLSQLLPELVKVLKDLGIADNCYFHVSDEPNLS
                     MLEDYKSSAMFLKEHLKGFKMFDALSSIEFYKEGLVTCPIPSLDHLGPFLEENIEELW
                     TYYCCGQVTVSNRFLAFPSSRNRLLGLILFKYDIKGFLQWGLN"
BASE COUNT          631 a          374 c          433 g          490 t
ORIGIN      
        1 aagacgacat ggcagacagg ataagtttgc ttgaaagcaa actggccgat ttggtagcca
       61 atggactaaa atatgcccaa gtatcccctg tagtggcagt ggaagaaaag gttgaaaaag
      121 tcgaggagaa aaaagaagaa aatattgtag agccaaagga agaaccagta aaagaagaga
      181 ttgttgaagc taggcccacg aaagagtcaa aaagcgcaaa acccattgac actctggatt
      241 ggaatgactt cttgcagaat attactgaca aaaacaaatc atccatatct gctttaataa
      301 gggtgaacag taaaggtttt gttcttgagc ctaatatact tataattgcc ttttcaaaca
      361 aaatgatgat ggacatggtt gtaagaaaca atgtgtttga catattggac gagtcagcgt
      421 caatggcctt tggaaaaccc atgaaagtaa agcttttaat ggaggacgaa ctggaagctt
      481 tcagcgcaag ttcagctgta gaagaaaatg agcaggaaaa taaggaaaag gacgacaact
      541 ttgaaaatgc actcaatcta ctcagtcaac tggctgagga tgaagggttt gaagtattca
      601 atattgagta aaggagtatt gccatgtttg atattttccc catcagctct ttagaaagag
      661 tatttatgga tcttcccttt gaagcaaagc cattacacaa ggcaacaatg ctgaaaaacg
      721 aatactattc ttttcagttt gcctacaagc taagtcatat ggcaccggca accaatatac
      781 cagtgcagct tgaggtgacc catgattttg gagattcatt aattgtaaag tttgtggatt
      841 acgtgccctg cgatttggct atctatgata acgccaccga accgctggaa cacccttggc
      901 ctggtctttt tccagacata ttaaggccga tgccaaaaca attgtgggcg ttcaaaaaga
      961 actacagaag cgtatggatt acaatcaacg gaaagaatat tgaaagtggt acctacacca
     1021 taacagctac tataagcagt ggggatacca aaaagagcac ttcctttgaa ttaacggttc
     1081 tggacaagga acttcccagg tacgacttca agtgcacaaa ctggttttat acggattgtc
     1141 tgtgcaacta ctacaacatc gagttcaatt ctgaagaata ctggaggata acggaggaat
     1201 ttgcaaagga agctgcaaga catggcgtca atatgctcct tacccccata ttcactcccc
     1261 cgctggatac cagagtgggt ggtgaaagaa gaaccatcca gcttgtggat gtatacaaaa
     1321 atggagacaa ctacaccttc aactttgaca agctggcaag atgggtgaac atgtgcaaaa
     1381 gggcaaatat cgaatattat gaaatatctc accttttcac ccaatggggc tgcaaacacg
     1441 ctcctaaaat aatgggatat gataatggag agtacaaaaa actatttggc tgggagacgg
     1501 atgcccacgg cagtgaatac agaagctttt tgtcacaact tcttcctgaa ctggtgaagg
     1561 tgctcaaaga tctgggaatt gcggacaatt gctacttcca tgtatccgac gagcctaacc
     1621 tatccatgct ggaagactat aaaagcagtg ccatgttctt gaaagagcat ttgaaaggct
     1681 tcaagatgtt tgatgctctc agttccatcg aattctataa agaaggactt gttacctgcc
     1741 ctattccgtc attggatcat ttggggccgt ttcttgagga aaacattgaa gaactctgga
     1801 cctattactg ctgcggccag gtgaccgtat caaacaggtt tcttgccttc ccttcatcca
     1861 ggaacagatt gcttggattg atactgttca agtacgacat taagggattc ttgcagtggg
     1921 gcctaaac
//
LOCUS       DAONVY010000454         1922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39848_length_1922_cov_2.955544, whole genome shotgun sequence.
ACCESSION   DAONVY010000454 DAONVY010000000
VERSION     DAONVY010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39848_length_1922_cov_2.955544"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="PLI11_10135"
     CDS             complement(<1..1827)
                     /locus_tag="PLI11_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPZ53267.1"
                     /translation="MKTSRFICMVLAVAMVLSLNIFQFESIHAEDKIYATTSSPAIPV
                     TVGEAINLEDVMVEFSSNVYFRASDVNITVTDDSKDTLKIENGKLTASEEGLHIIMVE
                     KNNIVKTVYVVARASADGDFVLFFDEFEAELLDGYRRVEGFSSDVYVENGFLFLKGNT
                     LRSPRLLLPEFLDDFGDYEIEVVGTITDAAEQTRWSSIMYRSQNNNAQYLQMCIRKGA
                     TANNGIEIAENTGGWTVHKTASYKEAISSDKMYTFKVHAEGPEINYYINSEKVLSGKL
                     EGYIKGGIGLQANNSTFKIDSLKVKYVAGKPGKAGYTFFEIVQPDMGIIGGMGLSEFV
                     ENQDDLTRIAELGMKPANVIFYIDKELNVTDNTYSNPYMGIEDAIMSLMGVMTPTFYI
                     NDEHAANNLGDFLKDNKLEDCFVMSGNPELVQIVRKKARMSRGVIDFTEKYKDKESVS
                     KDDLMEIRGIVNSNMASVCVIPSNIANRVNVKFLYDRLVSVWVNAVNDLTQKKDAYSL
                     LITGAHGIISDNTHLVYETAMLMSGSKLLRVPLNVGHRGVPSLAPENTIEGALLAYEN
                     GADVIEIDIHLTKDGVPVIIHDANTARTCNGVSREVRNSTVEQ"
BASE COUNT          529 a          458 c          318 g          617 t
ORIGIN      
        1 ctgttccact gttgaatttc tgacctcacg gctgacaccg ttgcatgttc tcgcagtgtt
       61 tgcatcatga attataacgg ggacaccgtc cttggtaagg tgaatgtcaa tttcaattac
      121 atccgcacca ttttcatatg caagcaaagc tccttctatg gtgttttcag gagcaaggga
      181 aggcactccc ctgtgaccta cattcaatgg aactctgaga agtttgcttc cgctcattaa
      241 cattgctgtt tcataaacaa ggtgcgtatt gtctgaaata atgccgtggg ctccggttat
      301 gagcaggctg taagcatctt ttttctgtgt aagatcattc actgcattaa cccaaacgga
      361 taccaatctg tcatatagga attttacatt gaccctgtta gcaatattcg atggaattac
      421 acatacagaa gccatgttgg aattaacaat acctctgatt tccataagat catctttcga
      481 taccgattct ttgtccttgt acttctcagt aaaatctatt actcctcttg acatccttgc
      541 tttcttacga acaatctgaa caagctctgg attgccgctc attacaaagc agtcttccaa
      601 cttgttgtct tttaaaaagt cacccagatt gtttgctgca tgctcgtcat ttatgtaaaa
      661 ggtaggagtc ataactccca taagactcat aatcgcatcc tcaataccca tgtatggatt
      721 tgagtaagtg ttgtcagtta cattaagttc cttgtctata taaaaaatta catttgcagg
      781 cttcatacca agctcggcta tccttgtcag atcatcctga ttctctacaa actcggacaa
      841 tcccataccc cctataatcc ccatatcagg ctgcacaatc tcaaagaacg tgtatccagc
      901 cttgccaggt ttgcctgcaa catacttaac ctttaaactg tctattttaa aggtcgagtt
      961 gttggcctgc aaaccaatac cgcccttgat atatccctcc agttttccag aaagcacttt
     1021 ttcgctgtta atatagtagt ttatttcagg accttctgca tgaaccttga aggtgtacat
     1081 cttgtcggaa gatatcgctt ctttatatga cgcagtttta tgaacagtcc atccgccagt
     1141 gttctcagct atttcaatac cattgttggc agttgctccc tttcttatac acatttgcag
     1201 atactgcgca ttattattct ggctacgata catgatggag gaccatctgg tttgttctgc
     1261 agcatcggta atagtgccga ccacttcaat ctcataatct ccaaaatcat caagaaactc
     1321 aggcagtaaa agccttggtg accgaagagt gttgcccttc aggaatagaa agccattttc
     1381 aacataaacg tcgctggaaa aaccctcgac acgcctgtac ccatccaaca gctctgcttc
     1441 aaattcatca aagaacaaca caaaatcgcc atctgcactt gcccttgcaa ccacatatac
     1501 tgttttcaca atattgtttt tttcaaccat gatgatatgt agcccttctt cacttgcggt
     1561 tagttttcca ttctctatct ttaatgtatc cttgctgtca tccgtaaccg taatgttgac
     1621 atccgaagct ctgaagtata cattggaaga aaactcaacc atcacatctt caagattaat
     1681 ggcctctcct accgtgacag gtattgcagg cgaagacgtt gttgcatata ttttgtcttc
     1741 agcatgaata ctttcaaatt gaaatatgtt cagtgacaaa accatggcta cagccagcac
     1801 catgcaaata aatctcgagg tcttcatact atcatccttt tttcgttatt tccatattat
     1861 cttaccatac ttaattttga ttttccatgg aaacaacttg cttgcatatt aaattatatc
     1921 ct
//
LOCUS       DAONVY010000455         1917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_39979_length_1917_cov_3.417293, whole genome shotgun sequence.
ACCESSION   DAONVY010000455 DAONVY010000000
VERSION     DAONVY010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39979_length_1917_cov_3.417293"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..658
                     /gene="thpR"
                     /locus_tag="PLI11_10140"
     CDS             137..658
                     /gene="thpR"
                     /locus_tag="PLI11_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPZ53268.1"
                     /translation="MRLFTAILFNDEVKDKLEKIKNTVRAYAIKGNFTLKNNLHLTLV
                     FLGEVEQCDVDSIVKALDKVEYNKFDMTLNRLGKFSRRDGDILWLGMDKENSDLARLR
                     KLVYRELGKLGFHQDKFTPHITLGRRVVLKEDLDSIQTNEISTRVDRISLMLSERVDN
                     VLTYTEIYSKNLH"
     gene            complement(690..1262)
                     /locus_tag="PLI11_10145"
     CDS             complement(690..1262)
                     /locus_tag="PLI11_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HPZ53269.1"
                     /translation="MWIYEKKLEFPINIKNPNPRLAKLIITQYGGPDGELAASLRYLS
                     QRFTMPTGETKAILTDIGTEELAHLEMIGTICSQLLKGADCETLKACGYDPAYVDHGC
                     GIYPMNASGVPFTAAYLQSKGDPETDLIENLAAEQKAKTTYEYLLHMTDDPDVIAPLR
                     FLREREVVHFQRFGEALRIVQDLKDRKTYY"
     gene            complement(1269..1532)
                     /locus_tag="PLI11_10150"
     CDS             complement(1269..1532)
                     /locus_tag="PLI11_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HPZ53270.1"
                     /translation="MDRDKMLHEIMALDFALIDLNLYLDTHPTCTETIELFNNVAKKR
                     KVMIESFEAMYGPLTAINYTNSEDMWDWVEEPWPWERKYGCKE"
     gene            complement(1535..1780)
                     /locus_tag="PLI11_10155"
     CDS             complement(1535..1780)
                     /locus_tag="PLI11_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HPZ53271.1"
                     /translation="MSDNRSNLCEKDYCTSPFMSTPLHEEDMRKMAVSDVTSQGRLAT
                     IYVPWQEPGKMFSICESLEKGTVYKDLYQPYKDRGCC"
BASE COUNT          560 a          372 c          368 g          617 t
ORIGIN      
        1 ctcttaatcc gtttacattc catgatacaa gtttcatttt ctacctccat aattctttac
       61 atcaatattc taacttattt tactcttgtt gtatataacc tgctggttag gtataatata
      121 gatggaggag acgattatga ggttatttac agcaattctt tttaatgatg aagtaaaaga
      181 caagttggag aaaatcaaga acacagttag agcatatgct ataaaaggaa actttacatt
      241 aaaaaacaac ttgcatctga cattggtctt cttgggtgag gttgaacagt gcgatgtaga
      301 ttctattgtg aaagcattgg ataaagtaga gtacaacaag tttgacatga ccttgaacag
      361 gcttggcaag ttttcaagac gggatggaga tattttatgg cttgggatgg acaaagagaa
      421 ttctgacctt gccaggcttc gcaaacttgt ctacagggaa cttggtaaat taggcttcca
      481 ccaagacaag ttcacaccac atattaccct gggcaggcgt gttgttttaa aagaggattt
      541 ggattcaata caaacgaatg aaatatcaac aagagtagac agaataagtc tcatgctgtc
      601 agagagagtt gataatgtat tgacttatac tgaaatatat tctaaaaacc tgcactaggc
      661 gaaaagcttt gtgcaggttt tggtatgggt tagtaatatg tctttctgtc tttcaagtct
      721 tgaacaattc ttagtgcttc accaaatctc tggaagtgca ctacctcgcg ttcgcgtaag
      781 aatctcaagg gagcaataac atcaggatca tcagtcatgt gtaacagata ctcatatgta
      841 gttttggctt tttgctcggc tgcaaggttt tctatcaggt cagtttcagg atcccctttt
      901 gattgtaggt atgctgcagt aaagggcaca cccgatgcat tcattgggta tatgccgcaa
      961 ccatggtcaa cgtatgcagg atcatagcca caggccttca atgtctcaca gtcagcacct
     1021 ttcaaaagct gcgaacaaat agttcctatc atttccaaat gtgccaattc ctctgtacct
     1081 atatcagtca gaatggcttt tgtctctcct gtgggcatag tgaacctttg acttagatat
     1141 ctcagtgatg cggcaagttc gccatcagga ccgccgtatt gtgtgatgat aagctttgca
     1201 agccttggat ttgggttttt aatgtttatt gggaactcaa gcttcttttc ataaatccac
     1261 attcaatatc actccttaca tccatatttt ctttcccaag gccatggttc ttcaacccaa
     1321 tcccacatat cctcggaatt cgtatagtta atagctgtca ggggcccata catggcttca
     1381 aagctttcaa tcatgacttt tctttttttc gcaacattat tgaacaattc tattgtttct
     1441 gtgcaggtgg ggtgagtatc cagataaagg ttcagatcta taagagcaaa atccagagcc
     1501 attatttcat gaagcatttt atctctatcc atgcctaaca gcaccccctg tctttatacg
     1561 gttggtacaa atctttataa acagtgccct tttccaggct ttcgcagata gagaacatct
     1621 tgcctggttc ctgccaaggc acataaattg tagccaatct accttgagat gttacgtctg
     1681 atactgccat ttttctcata tcctcttcat gaagtggagt actcatgaaa ggggaagtgc
     1741 aataatcctt ttcacataag ttggaacgat tgtcgctcat taattctatt cctccataaa
     1801 tataattccg ttatattata ttaaccatgt atgtttagtg tcacattagc atgagataat
     1861 tatcgttatt tagtgctgtg agtaacagaa atttacaaat aaaaaaacag gagattt
//
LOCUS       DAONVY010000456         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40073_length_1913_cov_4.066738, whole genome shotgun sequence.
ACCESSION   DAONVY010000456 DAONVY010000000
VERSION     DAONVY010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40073_length_1913_cov_4.066738"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..535)
                     /gene="trmL"
                     /locus_tag="PLI11_10160"
     CDS             complement(41..535)
                     /gene="trmL"
                     /locus_tag="PLI11_10160"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HPZ53272.1"
                     /translation="MAFNIVLVEPEIPQNTGNIVRTCACTGATLHLVGPLGFSISDKH
                     LKRAGLDYWDDMDIRLYDSFEEVVGKSNGGTFYFCTTKAKRTYKEVEYKDGDYLVFGK
                     ETKGLPESILQANYEWCIRIPMLKDYRSLNLSNSVAIVLYEAVSQLGFPGMLMQGELT
                     GRGD"
     gene            complement(681..1313)
                     /locus_tag="PLI11_10165"
     CDS             complement(681..1313)
                     /locus_tag="PLI11_10165"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPZ53273.1"
                     /translation="MRHAKSKNLLGQGLALIAGYDIFRTETITEEVERVNEVEEKKFK
                     EKEKRERKMRGENNGNNTDTVISKETILNGSISSNSNIKVYGTVKGNITSDNNVIILG
                     EVEGDITGKSVEILNGNVVGNIKSETMVKISDSSVLKGDIVCQSLELNGQVGGNALVH
                     NTATLGDCAVIYGNIESQNLSIQEGAIVDGTIKVVKDNTHIGGVLAEDAI"
     gene            complement(1423..>1913)
                     /locus_tag="PLI11_10170"
     CDS             complement(1423..>1913)
                     /locus_tag="PLI11_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPZ53274.1"
                     /translation="VEPDVVIMDLQMPKCNGLTATKLIKSEKPHVIIVILSMSDDEKN
                     LFNAIKNGASGYLLKSLNPNEFIQELSSIIANGSVLLQETALQVAEELESQFDPVSML
                     SQRQIAVLTLVAEGKTYKEVASELYVSERTVKYLMAEILKILNLKNKNEAIIYARKCG
                     LA"
BASE COUNT          535 a          464 c          260 g          654 t
ORIGIN      
        1 ttttcaacct ggttttatac ataataaaat ctcctttatt ctaatctccc ctacctgtca
       61 actcaccctg catcagcatt cctggaaatc ccagctgcga tacagcttca tacaacacaa
      121 tagcaacaga gttggaaaga ttgagcgacc tgtaatcctt caacatgggg attcttatac
      181 accattcata gttagcctgt aaaattgatt cgggaagccc tttggtttcc ttcccaaaca
      241 caagataatc tccgtccttg tactcaactt ccttgtatgt tctttttgcc tttgtagtgc
      301 aaaaataaaa agttccaccg tttgacttcc caactacctc ttcaaaggaa tcatacaacc
      361 ttatatccat atcgtcccaa taatcaagac ccgctctttt caaatgtttg tcggatatcg
      421 agaaccccaa aggacccacc aaatgcaaag tcgcccctgt acatgcacag gttctgacaa
      481 tatttccagt attctgaggt atttcaggtt caaccaacac aatattaaat gccatgcaaa
      541 cccttctttt caaatataat actttaaaat tatactacat ggaaatatta ttcacaagta
      601 taaaaacaga atcggcagac gctttacgca tctgccgatt ctacttggac aatatatcaa
      661 tattcttcga acttacactg tcatatcgca tcttctgcga gcactccgcc tatatgggta
      721 ttatccttca caaccttgat cgttccgtca actattgcgc cttcctgaat tgagaggttt
      781 tgtgattcaa tatttccata tattacagca caatccccca aggttgcagt gttgtgaacc
      841 aaggcgtttc ctcccacttg tccattcagt tctagtgatt ggcaaacaat atcacctttc
      901 aatactgaac tgtcagatat ttttaccatt gtttcactct ttatatttcc aacaacattt
      961 ccatttagaa tctcaacaga ttttccggta atatctcctt caacttcacc caatataatt
     1021 acattgttat cgcttgtaat atttcctttc acagtcccat aaaccttaat gttactgtta
     1081 ctggaaattg aaccatttaa aattgtctcc ttggatataa cggtatctgt attgtttcca
     1141 ttattctctc ctctcatttt tcgttccctt ttttccttct ccttaaactt tttttcttca
     1201 acttcgttta cccgttcgac ttcttctgta attgtttcag ttctaaagat gtcgtaaccc
     1261 gcaattaacg ccaatccttg ccccaataaa ttcttcgact tagcatgtct catagctcat
     1321 tctctcctta ttctctttag actcttcgct tatattgtaa tacttatttt gttcttaatg
     1381 ggggcaatta tagtgcaaaa aagggcaaca taagggcaat ctttacgcca atccacactt
     1441 tctggcataa attatagctt catttttgtt tttcaagttt aaaattttta atatttcagc
     1501 cattagatac tttaccgtcc tttcactaac ataaagttct gatgccacct ctttgtaagt
     1561 tttgccctcc gctacaagag taagaactgc tatttgtctc tgagacaaca tcgatacagg
     1621 atcaaattgc gactcaagtt cctctgccac ttgtaatgca gtctcctgta ataaaaccga
     1681 accattggca ataatgctgc tgagctcctg aatgaactcg tttggattca gactctttaa
     1741 aagatatcct gaagcaccat tcttgattgc attaaaaaga ttcttttcat catccgacat
     1801 acttaagata acaataatca catggggctt ttcactttta atgagctttg ttgccgtaag
     1861 tccgttgcac tttggcattt gaaggtccat aatcaccaca tccggctcca cca
//
LOCUS       DAONVY010000457         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40078_length_1913_cov_3.889128, whole genome shotgun sequence.
ACCESSION   DAONVY010000457 DAONVY010000000
VERSION     DAONVY010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40078_length_1913_cov_3.889128"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLI11_10175"
     CDS             complement(<1..742)
                     /locus_tag="PLI11_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53275.1"
                     /translation="MDTKGSIEVLPSHVVDALRAHDIDASDIKVSVKSDMTLDNVYMD
                     SWVFITGERLVLMDGLVHKSGKKKGKKAAEINENWEEKSYTEYPITEFKDFKVEILPA
                     TNIITANRNGDDFVVCKFTNTLGKKMRAFTELANKVVEGKEITEEDLAGHDNESSYCP
                     KCGKLYRNQEEKICIHCIDRKKVFFRLLNYGSQYKWYMVAIVIFILISSGLALLYPYL
                     GNKIFYDEILSESGKYYGKVVMLVGLILC"
     gene            complement(804..1532)
                     /locus_tag="PLI11_10180"
     CDS             complement(804..1532)
                     /locus_tag="PLI11_10180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPZ53276.1"
                     /translation="MERIRLQKYIAMCGVCSRRDAEELIKQGRVYVNGSKVEEMGLKI
                     TYEDKVEIDGNVISMEKKKYYIMMNKPTGVISAVSDDRGRKCVTDFIKGVDARLYPVG
                     RLDYDTQGLILITNDGDFMQKLTHPSHEVWKTYEALVRGIPDQNDIKEFEQGLLLEDG
                     MTLPCVLEVIGYEGKNAIVEVMIREGRNRQVRRMLERINHPVIRLKRTKIASLELGDL
                     KPGQWRFLNSEDFKALNMSDREQA"
     gene            1663..>1913
                     /locus_tag="PLI11_10185"
     CDS             1663..>1913
                     /locus_tag="PLI11_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53277.1"
                     /translation="MKIWIQAEFGDDIHIFIHIMLFGIIIKTYKIPSAPKKIKKKKKK
                     RKAKFKGTEITKILWESTKKRKIEYLEFKGDLGLADPALT"
BASE COUNT          560 a          431 c          278 g          644 t
ORIGIN      
        1 ggcaaagtat aagtccgaca agcattacaa ccttgccata gtatttgcca ctttcactta
       61 gaatctcgtc gtaaaatatc ttgttgccca ggtaaggata cagtagagcc agtcctgaag
      121 aaataagaat gaagatgaca attgccacca tataccattt gtattgactt ccataattaa
      181 gaagtctaaa aaacaccttt tttctatcta tgcagtgtat gcaaatcttc tcttcctgat
      241 ttctgtatag ttttccgcac ttgggacagt atgagctttc attatcatgg cctgccaaat
      301 cctcctcggt tatttccttt ccctcaacaa ctttgttggc aagctctgtg aaagcacgca
      361 ttttctttcc aagggtgttt gtaaatttgc acacaacaaa atcatcgcca tttctgttgg
      421 cggttatgat gtttgttgca ggcaatattt caaccttgaa atccttgaat tctgttattg
      481 ggtattccgt atagcttttt tcttcccagt tttcatttat ctcggcagct tttttgcctt
      541 tcttctttcc actcttatgt accaagccat ccattaaaac caagcgttcc ccggttataa
      601 atacccacga atccatatac acattatcaa gagtcatatc cgacttaaca ctgaccttta
      661 tgtcgctggc atctatatca tgggctctaa gggcatcaac aacatgtgat ggcagtactt
      721 ctatagaccc tttagtatcc attttacacc tgaactttct tacaaatcgc ttttaataat
      781 aaccatccct ggcataagtc atcttatgcc tgttccctat ctgacatgtt taaagccttg
      841 aaatcttcac tatttaaaaa cctccactgg cctggcttca aatcacccag ctccagggat
      901 gctatcttcg ttcttttcaa tctgattacc ggatggttta ttcgctcaag catccttcga
      961 acctgtctgt ttctgccttc tctaatcata acttctacaa tagcgttttt cccctcatat
     1021 cctattacct ccaatacaca aggcagcgtc atgccatctt ctagcagtaa accttgttca
     1081 aactccttta tgtcgttttg atcaggaata cctcttacca aagcctcata tgttttccat
     1141 acttcatggg atggatgagt taatttctgc atgaaatccc catcattggt tattaaaatc
     1201 aatccttgcg tgtcataatc caacctgcct acaggataaa gtcttgcatc aactcccttt
     1261 ataaaatcag taacacattt tcttcctctg tcatcactca cagcacttat tacacctgta
     1321 ggtttgttca tcattatata gtattttttc ttttccatac tgattacatt accatctatt
     1381 tcaaccttgt cttcataagt aatcttcaat cccatttctt ctactttact gccatttacg
     1441 tatactctgc cttgttttat aagttcttcg gcatcccgtc ttgagcaaac accacacatg
     1501 gcaatatatt tttgcaacct aattctttcc ataaatatcc cttttcctta tttcatgcac
     1561 attatacatg aacaattttt taattgcaag tataaacaag gaaaataatg tgcaatatag
     1621 tcatgggctt acaatttttt catatggaag agggaggttt tcttgaaaat ctggatacag
     1681 gcagaatttg gtgatgacat ccatattttt atacatatta tgctatttgg cataataata
     1741 aagacataca agataccaag cgctcccaaa aagataaaaa agaagaagaa aaaaagaaaa
     1801 gcaaaattca aaggaactga aattaccaaa attctatggg aaagcacaaa gaaaagaaaa
     1861 atcgaatact tggagtttaa aggagatttg ggtctagcag atccggcatt gac
//
LOCUS       DAONVY010000458         1910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40176_length_1910_cov_3.130458, whole genome shotgun sequence.
ACCESSION   DAONVY010000458 DAONVY010000000
VERSION     DAONVY010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40176_length_1910_cov_3.130458"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="atpA"
                     /locus_tag="PLI11_10190"
     CDS             <1..1089
                     /gene="atpA"
                     /locus_tag="PLI11_10190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPZ53278.1"
                     /translation="VYKPLETGILAIDSMFPIGHGQRELIIGDRQTGKTTIAVDTILN
                     QKDKDVICIYVAIGQKASSVAYIVNTLEKHGAMEYTIVVNAPASDPAPLQYIAPYAGC
                     ALGEFFMNKGKAVLIVYDDLTKHATAYRSLSLLLERTPGREAYPGDVFYLHSRLLERS
                     AQLSNEKGGGSMTALPIVETQAGDVSAYIPTNILSITDGQIYLDTELFFAGQRPAIDI
                     GLSVSRVGGDAQTKAMKKAVGALRLDLAQYKEMEIFMQFSSDLDEMTKRQLEYGQGLM
                     HLLRQEKNRPMKLYEQVIILIAALNKVMLGIRINEINEFKYGLLEYIEKNSPSICNEI
                     NTSGNFKNSTKDEILKLAKSYLRSFDDK"
     gene            1086..>1910
                     /gene="atpG"
                     /locus_tag="PLI11_10195"
     CDS             1086..>1910
                     /gene="atpG"
                     /locus_tag="PLI11_10195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPZ53279.1"
                     /translation="MMNAKEIKARIKSVQDTRKITNAMYMIASTKMRKARNDLDKTRP
                     YFKALYEEMEHIFKSVEDIESRYVCTSAGNGDNGKTYGVLVITADKGLAGSYNKNVIK
                     EAMELISKYKINKLFVVGEYGRRFFMQRDINIEKSFLYTAQNPTMDRAREIAALLLDM
                     YDKGELDEIYIIYSDMKNSLATKVIQRRLLPFQRTHFHSIEDEKVQIFEVFPSVSVVV
                     DSLVKSWLSGFIYSALVESFCSEQSSRMAAMYSANKNAEKIMETLTIQYNLVRQANI"
BASE COUNT          624 a          312 c          454 g          520 t
ORIGIN      
        1 gtttacaaac cgctggagac aggtattctg gcaatagatt caatgtttcc aattggtcac
       61 ggtcagaggg aactgattat aggggatagg caaacaggca agaccaccat cgcagtagat
      121 accatactga accaaaaaga caaggatgta atttgcatct atgttgctat tgggcaaaag
      181 gccagttcag ttgcctacat tgtaaacacc cttgaaaagc atggcgccat ggagtataca
      241 attgttgtta atgcacctgc aagcgatcct gcacctcttc aatacatagc accttatgca
      301 ggttgcgctt tgggcgagtt cttcatgaat aaaggcaaag cagttttgat tgtttatgat
      361 gatttgacaa agcatgcaac tgcatatcgt tcgttgagtc tgttattgga aagaactcct
      421 gggagggaag cgtatcctgg tgatgtgttt tatctacatt ctcgtctgct tgaacgctcg
      481 gcacagctct ccaatgaaaa gggaggaggc agcatgactg ctttgccaat agttgaaaca
      541 caggcaggag atgtgtctgc atatattcca acaaatattt tatccattac ggacggacag
      601 atttatctgg acacagagtt gttttttgca ggacagcgtc cagccattga tattggtttg
      661 tctgtgtcaa gggttggtgg tgatgctcaa actaaagcta tgaagaaagc agttggagct
      721 ttaaggcttg acttggcaca gtacaaggaa atggagattt ttatgcagtt ttccagtgac
      781 ttggatgaga tgacaaaaag gcagcttgag tatggtcagg gactgatgca tttgctgaga
      841 caggaaaaaa acagaccaat gaagttgtat gagcaggtca taatccttat tgcggcactg
      901 aacaaggtta tgctgggtat acggattaat gagataaatg aattcaagta tgggcttctg
      961 gaatacattg agaaaaactc accaagtata tgtaatgaga taaatacaag tggtaatttt
     1021 aaaaattcaa caaaagatga aatattaaaa cttgccaaat catatttacg gagctttgat
     1081 gacaaatgat gaatgcgaag gaaataaagg ctaggataaa gagtgttcag gatacaagga
     1141 aaattaccaa tgcaatgtat atgattgctt caacaaagat gagaaaagcc agaaatgatc
     1201 ttgataaaac gaggccatac tttaaagctt tgtatgaaga gatggagcac atttttaaat
     1261 cagtagaaga tattgagagc aggtatgttt gcacgtcggc tggcaatggt gataatggca
     1321 aaacttacgg agtgcttgta attactgcgg acaagggtct tgcaggctca tataacaaga
     1381 acgttataaa agaggcgatg gaactaatat cgaagtataa aatcaacaag ctttttgtgg
     1441 tgggggaata cggcagaagg ttctttatgc aaagagatat aaatattgaa aaaagctttt
     1501 tgtacactgc gcaaaaccct accatggaca gggcaagaga gattgccgct ttattattgg
     1561 acatgtatga caaaggtgaa ctggatgaaa tatatatcat ctacagcgac atgaaaaaca
     1621 gtcttgcaac caaggtaata caaagacgtc ttttgccttt ccaacggaca cattttcaca
     1681 gtatcgaaga tgaaaaagtg caaatatttg aagttttccc ttctgtgtct gtggtggtag
     1741 acagtcttgt gaagagctgg ttgtcaggct ttatttacag tgcattggtt gagagttttt
     1801 gtagcgagca aagctcgcgc atggctgcca tgtattctgc aaacaagaat gcagaaaaga
     1861 taatggagac tttgaccatt caatacaatc ttgtacgtca ggcaaatatt
//
LOCUS       DAONVY010000459         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40341_length_1904_cov_3.440238, whole genome shotgun sequence.
ACCESSION   DAONVY010000459 DAONVY010000000
VERSION     DAONVY010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40341_length_1904_cov_3.440238"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLI11_10200"
     CDS             <1..804
                     /locus_tag="PLI11_10200"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPZ53280.1"
                     /translation="YVEKLEEASSVDITNLDDLKLALRKRMEFFNEHGCRLSDHAMDP
                     VYFEECDDEKADKTLKKALAGESLARHEVEGYKTNVILFLGREYARLNWTMQLHIGVI
                     RNVNSRMMEKLGPDTGFDCIGDYSFAKGLCSLLDSLDRTNKLPRTILYCINPRDNELL
                     ASIIGCFQRDVPCKIQFGSGWWFNDQKDGMIRQMTALANLGLLSRFVGMLTDSRSFLS
                     YTRHEYFRRILCNFLGGQMEAGEIPKDFGLVGKMVQDICYNNAKRYFGF"
     gene            complement(913..1466)
                     /locus_tag="PLI11_10205"
                     /pseudo
     CDS             complement(913..1466)
                     /locus_tag="PLI11_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687796.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1535..>1904)
                     /locus_tag="PLI11_10210"
     CDS             complement(1535..>1904)
                     /locus_tag="PLI11_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53281.1"
                     /translation="DDIQNDTNNSMTLFINSGQGNQGWKGYDFVVNRLKGSSGLVIEK
                     FANGYSTEKVGEAEYKKEGNEMHLRISKELLGIKGKIIDIQFKWADNFNDDDIFSFYT
                     DGDCAPYGRMNYLFSNKAKG"
BASE COUNT          529 a          359 c          403 g          613 t
ORIGIN      
        1 tacgtcgaga agcttgaaga agcttcaagc gtggatatca ccaatctaga tgatctaaaa
       61 cttgccctga gaaagagaat ggagtttttc aacgagcatg gctgcaggct ttctgaccat
      121 gccatggatc ctgtgtactt tgaggaatgt gatgatgaaa aagctgacaa gacgctcaag
      181 aaagcactgg ctggagaaag tcttgcaagg catgaagttg aaggatacaa gacaaacgtt
      241 attcttttcc ttggcaggga atacgcaaga ctgaattgga caatgcagct tcatattggc
      301 gtaataagga atgtgaacag caggatgatg gagaagctgg gtcctgacac gggttttgat
      361 tgtattggag actactcctt tgcaaaagga ctgtgcagtc tgctggacag tcttgacaga
      421 actaacaagc ttccaagaac cattctatat tgcataaatc caagagacaa tgaacttctg
      481 gcatcaatta ttgggtgttt ccagagagat gtgccgtgta agatacagtt tggttcaggt
      541 tggtggttca atgaccagaa ggatggcatg attcgtcaga tgactgctct tgcaaacctt
      601 ggcttgctca gcaggtttgt aggtatgctt actgattcaa gaagttttct ttcatatacc
      661 aggcatgagt atttcagaag gattctgtgt aattttctgg gagggcagat ggaagcaggt
      721 gaaataccga aagactttgg tcttgttggt aaaatggtac aggatatatg ctacaataac
      781 gcaaaaagat attttggatt ttgatagtaa ttctcccatc ctgtatagga tgggagaaaa
      841 atttcctgtt ttgtattatt cagaagaatt atccagattt actgttatat agataaatct
      901 gattcaatac aagaaacgac atgacgcaag acggtggctt atgaatattg cggttttgtt
      961 ttcaacaatt ttgttaaaac gggtgtatac ttcgtattca gctaccggat ccaatgctgc
     1021 agtcggctca tccagaatta taaatgcgct atttttgtag agtgttcgtg ccagtgctat
     1081 cttctgagcc tctccgctgg atatctcaac tccgttatcg ctaaaatcct tgtaaaggta
     1141 tgtatccatt ttgctccacc tatcgttgaa accggctttg gcaagacatg aaaccgcata
     1201 atcttcgtca aattcaactc tggatgccac gttctgtaca agtgtgaagg caagcaggtt
     1261 gaaatcctga aaaactacag agaaaatgga catgtattcc ttgtagttgt attttcttat
     1321 atctattcct taagcagaat ctcgccttcg gttggatcgt aaagcctgca taataacttt
     1381 ataaatgtta tttttccaga gccgtttttg cctacaactg caagtttttc tccttttcgg
     1441 aacttgatgg atacattttt taacgcatta gtgtttgaag aagggtagtc atcgcaaatt
     1501 gctatcttgt acattgaacc atcccttttt tattctatcc ttttgccttg ttgctgaaaa
     1561 ggtagttcat tctgccgtac ggagcgcagt ctccatcggt atagaaggag aaaatgtcat
     1621 catcattgaa attgtcagcc cacttaaatt ggatgtcaat tatctttcct ttaattccaa
     1681 gtaattcttt gctgattctt agatgcattt cattgccttc ttttttgtat tctgcttctc
     1741 ccactttttc agtagagtag ccgtttgcaa acttctcgat tacaagacca ctgctgccct
     1801 ttagcctgtt gacaacaaaa tcgtatcctt tccatccctg atttccttga ccggaattaa
     1861 taaacaaggt catgctgttg tttgtatcat tttgtatgtc atct
//
LOCUS       DAONVY010000460         1902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40404_length_1902_cov_2.950731, whole genome shotgun sequence.
ACCESSION   DAONVY010000460 DAONVY010000000
VERSION     DAONVY010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40404_length_1902_cov_2.950731"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLI11_10215"
     CDS             <1..269
                     /locus_tag="PLI11_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPZ53282.1"
                     /translation="GASTITSSKASFTVNGTGNPGMAVCGSGDVLCGIIAAFATRMNL
                     YDAARYGVLVHGLCGDKARELYGEESMLSGDLINMLPQVLRIDW"
     gene            279..1457
                     /gene="alr"
                     /locus_tag="PLI11_10220"
     CDS             279..1457
                     /gene="alr"
                     /locus_tag="PLI11_10220"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPZ53283.1"
                     /translation="MDFRYNRAWAEIDLDAISNNVREVRRITKPNAEIMGIVKADGYG
                     HGAMEVANTLIDNGVSRLGVAILDEAIQLRKNGIEVPILVLSYTDPRRAKELIKYDIT
                     QTIYSHELAKYLSDVALAENATIKVHVKVDTGMGRVGFLPGYSAVKDVMKISSLKGLI
                     VEGLFTHFAQADTEDRTYTRNQFEMFMSIVNELKRIGIHIPILHVANSGAIVQYPSMH
                     LDMVRAGIILYGHYPSEYTRKYDLNLKPAMTLKAGVNMVKTVEKDTSISYGSTYRTDA
                     KRTIATITIGYADGYSRLLSGKSKVLINGEYAPIVGRICMDQCMADVTDFEKKPEIGD
                     EVVLFGSQMNKSITCEELADIMGTINYEIISLVGKRVPRIYIKNGEIINVLNYICPDN
                     "
     gene            1567..1839
                     /locus_tag="PLI11_10225"
     CDS             1567..1839
                     /locus_tag="PLI11_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53284.1"
                     /translation="MPEFKKVLITISSDLLNEAEALAKENNSSLDQVVQTALHDLLKK
                     TKQEELVRQMEKGYQEMADINLKIAEEFLHIENEVMSILESDDKTA"
BASE COUNT          640 a          279 c          468 g          515 t
ORIGIN      
        1 aaggcgcatc aaccataaca agcagcaagg cttcatttac ggtaaacggt acaggtaatc
       61 ctggaatggc tgtatgcggt tccggtgatg tactttgcgg tataatcgct gcatttgcta
      121 caagaatgaa tctatatgat gctgcaagat atggagtgtt ggttcatggt ctttgtggag
      181 acaaggcaag ggagctttat ggagaagaat caatgctttc aggcgacttg attaatatgc
      241 ttccacaggt tttgagaata gattggtgag gtaattgtat ggatttcagg tataacaggg
      301 cgtgggcgga aattgatttg gatgcgataa gcaataatgt cagggaagtt agacgtatta
      361 ccaaacctaa tgcggaaatt atggggattg tgaaggctga cggatatggc catggcgcca
      421 tggaggtcgc aaacacgctt attgataatg gagtatcaag gcttggagtt gctattttgg
      481 atgaagccat acagctcaga aagaatggaa tagaggtacc aatattggtt ctaagctata
      541 cagatccgag aagagccaag gaacttatta agtatgatat aactcagaca atatacagcc
      601 atgaactggc caaatatttg tcggatgtgg ctttggctga aaacgcaaca atcaaggttc
      661 atgtaaaagt agatacggga atgggaaggg ttggattcct gcctggttat agtgctgtaa
      721 aggatgtaat gaagatatct tcattaaaag gactaatcgt ggaaggtttg tttacccact
      781 ttgctcaggc ggacacggaa gacaggactt acacccgtaa tcagtttgag atgttcatga
      841 gcatagtaaa tgaactaaag aggataggca tacacatacc tattttacat gttgcaaaca
      901 gtggtgccat agttcagtat ccctcaatgc atcttgacat ggtacgggca ggtattattc
      961 tgtacggaca ttacccgtcc gaatatacca gaaagtatga tttgaatcta aagcctgcaa
     1021 tgacattaaa ggctggcgtg aatatggtca agactgtgga aaaggataca agtataagtt
     1081 atgggagtac atacagaaca gatgctaaaa gaacaatagc aacaatcacc attggatatg
     1141 cagacggata ttcaagactt ttaagcggca aatcaaaggt acttataaat ggggagtatg
     1201 caccgatagt gggaaggatt tgcatggatc agtgcatggc ggatgttaca gattttgaaa
     1261 agaaacctga gattggtgat gaagttgttc tatttggttc gcagatgaat aaaagtataa
     1321 catgtgagga actggcagac ataatgggga cgataaatta tgagataatt agtttggttg
     1381 gcaaaagggt ccctcgcatt tacataaaga atggcgagat tatcaatgtc ctaaactata
     1441 tttgtcctga caattagtga cattgacttt ggaatggtag gaaaataaaa taaaattgaa
     1501 aaatttttat tgataatgta ttcacttcgg aaaaaatgga tacactttag ttggagggtt
     1561 tggttaatgc ctgaattcaa aaaagtactt attactattt catctgactt acttaatgaa
     1621 gcagaagccc tggcaaagga gaacaattct tctttggatc aagttgttca aacagcattg
     1681 catgatcttc ttaagaagac aaagcaggaa gaactggtca ggcaaatgga aaaaggctat
     1741 caggaaatgg cagatataaa tctaaagata gctgaggagt ttctgcatat tgaaaatgaa
     1801 gtaatgtcta tattggagtc agatgataaa acggcataaa acctccaaaa acataagtgg
     1861 gagtgaatct tgtgatcaaa agaggagata tttattacgc gg
//
LOCUS       DAONVY010000461         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40496_length_1899_cov_2.457158, whole genome shotgun sequence.
ACCESSION   DAONVY010000461 DAONVY010000000
VERSION     DAONVY010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40496_length_1899_cov_2.457158"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLI11_10230"
     CDS             complement(<1..528)
                     /locus_tag="PLI11_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylase"
                     /protein_id="HPZ53285.1"
                     /translation="MVPFNSDRKHKNRKVEPETMDRIYDMVKTQYKTGPVMKYDDYLT
                     DSPSIFKHEGNWYMMYITISKDVSVSGYETHLAKSKDLIDWEQYCTILRRNDRNRWDS
                     KQCAGYAAFVDIEFGKTNEIRKVDDRYYFSYLAGNSDGYEPDPLYMGLAYSVDPTDPS
                     SVTRLEEPILRPDDVD"
     gene            complement(614..820)
                     /locus_tag="PLI11_10235"
     CDS             complement(614..820)
                     /locus_tag="PLI11_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPZ53286.1"
                     /translation="MKNTIINIGGLATSEDASRIASILGTLDGVMNVNIDLNGRAASV
                     DYEPSVTSLQVIKHKIEEMGYKTL"
     gene            complement(937..1683)
                     /locus_tag="PLI11_10240"
     CDS             complement(937..1683)
                     /locus_tag="PLI11_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53287.1"
                     /translation="MKKLILTTLLFLFVSSIISSHAVVHTIYFDWEELGAKKIGTNAV
                     EEDYFGENVHNLRCNYSSWDHAQEYLWLVSDWSRQRREPGWIYIGKHDLSVIDTISFF
                     YVTDGSPDMTNNKVSLTKDKEGTQVVATAKIEEATLPLAEPKNLQMEILEPDYNGDLY
                     VYIEYSARMFVGNLEFVTKDDGTTPTPTKTPTPEPQTTEKPDKTQGQTKEPITETDGD
                     GVNFYVVGAVAGICVLGAVAILIIWRTKKK"
     assembly_gap    1739..1748
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          472 a          434 c          344 g          639 t
ORIGIN      
        1 atcaacatca tcgggtctta aaatgggctc ttccagcctt gtaaccgaag atggatctgt
       61 cgggtcaacg gagtacgcaa gacccatgta taaaggatca ggttcatatc cgtcactgtt
      121 tcctgccagg taagaaaaat agtatctgtc gtccactttt cttatctcat tggtttttcc
      181 aaactcaata tccacaaatg ccgcatatcc tgcacactgc ttgctgtccc atctgtttct
      241 atcatttctt ctaagtattg tgcaatactg ctcccagtcg attaagtctt ttgattttgc
      301 aagatgagtc tcatatccgg aaacgctgac atcctttgat atggttatgt acatcatgta
      361 ccagtttccc tcatgcttga aaatggaggg actgtcggtc aggtagtcgt catacttcat
      421 aaccggccct gttttatatt gtgtctttac catgtcatat atcctgtcca tggtttctgg
      481 ttcaactttc ctgttcttgt gctttctgtc tgaattaaac ggtaccatta acctcacctt
      541 cctctgttgc tcggaccaat tatataactt atgcaaaaag cgcgcaacca ataaacagtt
      601 gcgcgcctct atattacagg gttttatacc ccatttcctc tattttgtgt tttataacct
      661 gcagagatgt taccgacggc tcgtaatcaa cagatgcggc tcttccattc aaatcaatat
      721 ttacattcat aacaccgtcc aaagttccca gaatgctggc tatcctgctg gcatcctcgg
      781 atgtggcaag cccacctata tttataatcg tatttttcat actttccctc caatatatct
      841 tttggtaata ttttgccttt gtattgcttt tttattcttc tgcataagaa aagagctgta
      901 cttttaaata cagcccttta catagtcgat attagttcat ttcttttttg ttctccaaat
      961 aattaatata gcaactgctc ccagtacaca gataccagct actgcaccaa caacataaaa
     1021 attaacacca tctccatccg tttctgtaat gggttctttg gtttgtcctt gtgtcttgtc
     1081 aggtttttca gttgtttgcg gctctggcgt tggagttttt gttggtgtgg gtgttgttcc
     1141 atcatccttg gttacaaact ccagattgcc aacaaacatc cttgctgagt attctatgta
     1201 cacatacaag tctccattgt aatccggctc gagaatctcc atttgcaaat tcttgggctc
     1261 agccaaaggc agtgtggctt cttcaatctt tgctgttgcc actacctgtg tcccttcctt
     1321 gtccttggta agggatactt tgttgtttgt catgtcagga gatccgtcag tgacatagaa
     1381 aaaactaatt gtgtcaatga ccgacaggtc atgcttgcca atatatatcc agcccggttc
     1441 tcttctttgt ctggaccagt cggaaaccag ccaaagatat tcctgagcgt gatcccagct
     1501 gctgtaattg cacctcaaat tgtggacatt ctctccaaaa tagtcttctt ctacggcatt
     1561 ggtgccaatt ttctttgcac caagttcctc ccagtcaaaa tatatggtgt gaactaccgc
     1621 atgactgctg ataatggaag agacgaatag aaatagcaat gtagttaata tcagtttctt
     1681 cataatacac atcctgctct cttcttaata atggcaagca gcatgggaat tactgcaann
     1741 nnnnnnnnaa tgtggcattt gcgtcaaatg tctcaggatt ctgaggttct tctgtatatg
     1801 gaggttcttg agtttcttcc attgtgagaa cgaagttgcc gacaaacatt ctaatatcat
     1861 agtcgatata caaatacatg ggtcctgtgt agcctgcat
//
LOCUS       DAONVY010000462         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40655_length_1893_cov_3.097388, whole genome shotgun sequence.
ACCESSION   DAONVY010000462 DAONVY010000000
VERSION     DAONVY010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40655_length_1893_cov_3.097388"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLI11_10245"
     CDS             complement(<1..336)
                     /locus_tag="PLI11_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPZ53288.1"
                     /translation="MEKVILARIGEIALKGLNRKNFENKLASNMRKAMKDCGEFVINW
                     SQSRFFIEGKDNFDIDKAVEKLTKVFGIVSVSVATCVENDMEEIKKAAHHVVEKKLGN
                     EAMTFKVEAK"
     gene            complement(348..1496)
                     /locus_tag="PLI11_10250"
     CDS             complement(348..1496)
                     /locus_tag="PLI11_10250"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPZ53289.1"
                     /translation="MREIYFDNSATTKQLNSVIRVMVHTAEKFYGNPSSLHSLGYEAE
                     KELRRNRQIFSDLLNCDEKEIVFTSGGSESNNLALIGYARANPRKGNHILVTETEHPS
                     VLECAKYLSDNGYNVEYIPVDRKGVPDLEWIRDKVKEKTVLVSVHHVNSETGSILPIK
                     EICKIVKGKNGNTVFHADCVQSFGKLKIDTKDLSCDMLSISAHKIGGPRGIGALYVKK
                     GIRVEPVIFGGGQEGGLRSGTENLPGIAGFAEAASIAYENLEEWFEKVREIRDYIAKE
                     LGKENKVHVISNLESSLPYILNVSFEGIKGEVLLHSLEAKGIYVSTGSACSTRKNKRS
                     HVLTAMQIPNNIIDGAIRISFSAQNTLDEAKEAVEIIKEEVSMLKRTR"
     gene            complement(1493..>1893)
                     /locus_tag="PLI11_10255"
     CDS             complement(1493..>1893)
                     /locus_tag="PLI11_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPZ53290.1"
                     /translation="RRSHLVILCGHYEGVDERVLEDIVDEEISVGDYVLTGGEIPAMV
                     LADCIGRLVPGVLSSEEAIMSESHVNGLLEYPQYTRPYEINGKKVPDILLSGHHAEIE
                     KWRREQSLERTKRKRPDLYEAYIKGENMDK"
BASE COUNT          487 a          477 c          287 g          642 t
ORIGIN      
        1 ctttgcctca accttgaagg tcatggcttc attaccaagc tttttctcaa caacatggtg
       61 cgccgccttc ttgatttcct ccatgtcgtt ttcaacacat gtagcaacac ttacagagac
      121 tataccaaac acctttgtca gtttttctac agccttatct atatcaaaat tatcttttcc
      181 ttcaataaag aaccttgact gcgaccagtt gattacaaat tctccacagt ccttcattgc
      241 cttcctcata ttcgatgcaa gtttgttttc aaagtttttt ctatttaatc cttttaaagc
      301 aatttctcca attctggcca aaataacttt ttccatatct attccaccta tctggttctt
      361 ttaagcattg atacttcctc tttgatgatt tccaccgctt cttttgcctc atccaatgta
      421 ttttgagcgc tgaaacttat tctaattgct ccgtcaataa tattattcgg tatttgcatt
      481 gcagtcagca catggcttct tttgttcttt ctggtagaac aggcagaacc cgttgacaca
      541 taaatccctt ttgcttcaag actatgcaaa agaacttctc cttttattcc ctcaaaggac
      601 acattaagaa tatatggcag actactctca aggtttgata tgacgtgaac cttgttctcc
      661 ttgcccagct cttttgcaat atagtccctt atctccctga ctttttcaaa ccattcttca
      721 agattctcat acgctatact agcagcttcg gcaaaccctg ctattccagg cagattctca
      781 gtacctgatc ttaatccacc ttcctgtcct ccaccaaata tcacaggctc aacacgtatt
      841 ccttttttaa catacagagc accaattcct cttgggccac ctatcttgtg ggcactaatg
      901 ctgagcatgt cacaacttaa atcctttgtg tctattttga gcttgccgaa actctgaaca
      961 caatcagcat gaaataccgt atttccattc ttgcccttga ctattttgca tatctctttt
     1021 attggcaaga tgctgcctgt ttcactatta acatggtgaa cagaaacaag aactgttttt
     1081 tctttaacct tgtctcttat ccattccaaa tcaggaactc cttttctatc aacaggaatg
     1141 tattccacat tatatccgtt gtcggacaga tatttggcgc attccaaaac agagggatgt
     1201 tccgtctcgg taaccaggat atgatttccc tttctggggt tggccctggc ataacctatg
     1261 agtgctagat tgtttgactc gctgccacct gaggtgaata caatttcctt ttcatcgcag
     1321 ttcaacaagt cggaaaaaat ctgccggttt ctcctgagct ccttttcagc ttcatatcca
     1381 agactgtgaa gagatgaagg attgccgtaa aatttttcgg cagtatggac catgactctg
     1441 ataacactat tcaattgttt cgttgttgca gaattgtcaa aatatatttc tctcatttgt
     1501 ccatattttc tcctttaatg tacgcctcat acaagtcagg tcttttcctt tttgttcttt
     1561 ccaaggactg ctcccttctc cacttttcaa tctctgcatg atggcctgaa agaagaatat
     1621 cgggcacctt ctttccgttt atctcataag gtctggtgta ttgaggatac tcaagcaagc
     1681 catttacatg ggattcggac attattgcct cctcagaaga caaaacgccc ggaacaagtc
     1741 ttccaataca atctgcaagt accatggcag gtatctcgcc acctgttaat acataatctc
     1801 caaccgaaat ctcttcatcc acaatgtctt caagaaccct ttcgtcaacc ccttcatagt
     1861 gtccgcaaag aatcacaaga tgagatcttc tac
//
LOCUS       DAONVY010000463         1889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40766_length_1889_cov_3.713740, whole genome shotgun sequence.
ACCESSION   DAONVY010000463 DAONVY010000000
VERSION     DAONVY010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40766_length_1889_cov_3.713740"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLI11_10260"
     CDS             <1..249
                     /locus_tag="PLI11_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPZ53291.1"
                     /translation="SMEVIIQEDPEYIFVVTMGDTENALKALKERVEKNPAWSELQAV
                     KNDRFIVLPKDLFHYKPNARWGESYEYVAKILYPEKFE"
     gene            272..1249
                     /locus_tag="PLI11_10265"
     CDS             272..1249
                     /locus_tag="PLI11_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPZ53292.1"
                     /translation="MVVALLVVLLIAGILLSISIGSTRIPISEIIEAIRKGDVTSKTY
                     RIVSFVRIPRTLAAVLAGCALAVSGTILQSVLNNSLASPSIIGVNSGAGLFTVLIAAF
                     FPASIYYTTIAAFLGALLAVLMVYFIARKTGASRMAIVLSGVAVSSFIGAITDTVLAL
                     KPDVSIERTAFLIGGLSGVTMEKLGFAGIFIAIALILSLILSYDMNVLALGDESAKSL
                     GMKVSRIRFLFLILAALLSGSAISFAGLLGFVGLIVPHVSRIIVGYDNRILIPVSALL
                     GGLFTLLCDLLARVLFAPFEIPVGIIMSFLGGPFFIYLLISRRRGQLYD"
     gene            1242..>1889
                     /locus_tag="PLI11_10270"
     CDS             1242..>1889
                     /locus_tag="PLI11_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ53293.1"
                     /translation="MIRLENVTAGYEKTAVVKDINIEFEEGTITSIIGKNGCGKTTLL
                     RTAANVLKPFSGKVSIHGEDVSEIPPKELAKKVSFLPQIRTVPNITVFNLVLHGRYPY
                     LGFSRTLQKKDKEIVNEAIFTMGLCDIADRNVLELSGGQRQKVYLAMVLAQDTDIVFL
                     DEPTTYLDINHQLEILEMIRKLKDMGKTVVMVLHDLNSALTYSGKVCLMHEGKIVC"
BASE COUNT          522 a          318 c          467 g          582 t
ORIGIN      
        1 agtatggaag taatcataca ggaagatcct gagtacatat ttgtagttac catgggtgat
       61 actgaaaatg cgctgaaggc gttgaaggag agggtggaga agaaccctgc ttggagcgag
      121 ctgcaagctg tcaagaatga cagattcatt gtgctgccaa aggatttgtt ccactataag
      181 ccaaatgcaa gatggggtga aagctatgaa tacgttgcga aaattctcta ccctgaaaag
      241 tttgagtaaa aacaagagga gctataactt gatggttgta gctctccttg ttgttttatt
      301 aattgctggt attttgctta gcataagcat tggatctaca agaataccca tttctgagat
      361 aattgaagcc atacgtaaag gagatgtaac ttccaaaacc tacaggattg taagttttgt
      421 aagaatacca aggactctgg ctgcagttct tgcaggctgt gccttggctg tttcaggaac
      481 tattctccag tctgttttga acaactccct tgcaagtccc agcataattg gtgtaaattc
      541 cggtgcaggt ctgtttactg ttttgatagc tgccttcttt cctgccagca tatattacac
      601 aaccattgcc gcttttttgg gtgctctctt ggcggttttg atggtatatt tcatagccag
      661 gaaaacaggt gcatccagaa tggcgatagt tctgtcagga gttgcggtat ccagctttat
      721 aggggcgata acggatacag tccttgcttt gaaacccgat gtttccatag aaaggacggc
      781 ttttttaatt ggaggtttgt caggagtgac aatggagaag ctgggttttg caggaatttt
      841 tatagctatt gcattaatac tctctttgat acttagttac gacatgaatg tattggcgct
      901 gggagatgaa agtgcaaagt cactagggat gaaagtttca agaatcagat ttttgttttt
      961 aatcctggca gctcttcttt ccggcagcgc cataagtttt gcaggtcttt tgggattcgt
     1021 cggacttatt gttccccacg tatcaagaat aattgttgga tatgacaaca gaatactgat
     1081 acctgtgtct gctttgctgg gtggtttgtt cacattgctg tgtgatcttt tggcaagggt
     1141 tctttttgct ccttttgaga ttcctgtggg aattatcatg tcgtttttgg gagggccatt
     1201 ctttatctat ctattaataa gtcgcaggag aggacagctt tatgattaga cttgagaatg
     1261 taactgcagg ctacgaaaag acagcagttg tcaaggatat taatattgaa tttgaagaag
     1321 gaacgataac cagcataatt gggaagaatg gctgcggaaa gactaccttg cttagaaccg
     1381 ctgccaatgt actaaaacct tttagcggaa aggtaagtat tcatggagag gatgtttcag
     1441 aaataccacc aaaggaactg gcaaagaaag tgtcctttct cccgcagatt aggacagttc
     1501 ccaatattac tgtgttcaat ttggttctgc acggtagata cccttatctt ggtttttcac
     1561 gcacgcttca gaagaaggac aaagagattg tgaatgaagc tatatttaca atgggtcttt
     1621 gcgatatagc ggataggaat gtgcttgaac tttcaggagg ccaaaggcag aaggtgtacc
     1681 tggctatggt tcttgcgcag gatacggata ttgtgttttt ggatgagccg actacgtatt
     1741 tggacataaa tcaccagctg gagatattgg aaatgataag gaagttgaag gatatgggga
     1801 aaaccgttgt aatggttttg catgacctta acagtgcttt aacttattca ggcaaggtgt
     1861 gtttaatgca tgaaggaaag atagtttgc
//
LOCUS       DAONVY010000464         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40793_length_1888_cov_3.522640, whole genome shotgun sequence.
ACCESSION   DAONVY010000464 DAONVY010000000
VERSION     DAONVY010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40793_length_1888_cov_3.522640"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLI11_10275"
     CDS             <1..994
                     /locus_tag="PLI11_10275"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPZ53294.1"
                     /translation="IVGYRSSRRFYIEKESRRGKNLPTRKLFKTLVYYSIPITLSSGI
                     QYGGNLIDASLIAGRLQAGGFSEVVSTSLYGDMIATRQLINVPTALVSALCVSILPAL
                     TASYARQDFKGTEERAGYGFKLLYIVAMPLSFAMTVFAKEIYTLLGFGNNHMLLTSLS
                     FSILLMGTVHLQNSIMQSVNRLFQSTMYLGISVLVKALLNYILIAIPTFNVYGAVIST
                     YIAYAIPLILNHRDLVKRVSIKVNPARRAVLPLIASFCMIVCAYVSNTVLNVLFSPIG
                     LANYLKVLVTFLPTALVGVYVYFKVLIFFGGITDEDLNSISPALVKIIRKVTKK"
     gene            1059..>1888
                     /locus_tag="PLI11_10280"
     CDS             1059..>1888
                     /locus_tag="PLI11_10280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPZ53295.1"
                     /translation="MDKISVVVPCYREEDALPHFWNALKKVMTEEFKDVEFEVLFVDD
                     GSSDGTLGILKKLAEEDSRVRYISFSRNFGKESAMYAGLENSTGDYVVVMDADMQDPP
                     ELLSEMYRTVKHDGYDCAATRRVNRIGEPKIRSFCARLFYKLMRKITKIDIVDGARDY
                     RLMSRQMVDAIISVGEYNRFSKGIFSWVGFNTKWLEYENVKRVDGETKWSFWKLLLYS
                     FEGIVAFSTVPLAISSIIGIILCLVSFAAIVFVVIRQLVWHNSAYGWASMVSIILMLS
                     G"
BASE COUNT          537 a          338 c          438 g          575 t
ORIGIN      
        1 tatcgttgga tacaggagtt ctcgaaggtt ttacattgaa aaggaaagca ggagaggcaa
       61 gaacctgcca accagaaagc ttttcaagac acttgtatat tacagtattc ccataacatt
      121 gagttccggc atacagtatg gaggcaattt aatagacgca tcattgattg ccggcaggct
      181 tcaggcagga ggattttcag aagtagtcag cacatccctt tatggcgata tgattgctac
      241 aagacagctt ataaatgtgc cgacggctct ggtttcagct ctttgtgtca gcattttgcc
      301 tgcacttaca gcctcctacg caagacagga cttcaaagga acggaagaaa gagcgggata
      361 cggcttcaaa cttttatata ttgtagctat gccgcttagt tttgctatga ctgtgtttgc
      421 aaaggaaata tatactttgc ttgggtttgg caacaatcac atgctgctta catccctgtc
      481 tttcagtatt ctcctgatgg gaaccgttca tcttcagaac tccatcatgc agagtgtaaa
      541 cagactgttc caaagtacaa tgtatttggg tatttcggtg cttgtaaaag cacttctcaa
      601 ctacatcctt atagcgatac ccacatttaa tgtatatgga gctgtgatat ctacatatat
      661 cgcatatgca atacctttga ttctgaatca cagggatttg gttaaaaggg tgtccataaa
      721 ggtcaatcct gcgagaaggg ctgttctgcc acttatcgca tccttttgca tgattgtgtg
      781 tgcttacgtt tcaaacactg tgttaaatgt tctgttttca cccattggtc ttgcgaatta
      841 tttgaaagta cttgtaacgt ttttgcccac ggctcttgtc ggagtttatg tttatttcaa
      901 agttctgata ttttttggtg gtataactga tgaggatttg aattcaatct cacctgctct
      961 tgtaaaaatc atacgcaagg tgacaaaaaa atagtgcttt gcaaataatg gataatatag
     1021 gtataatatg ttgtaaaatt tagttagaga ggatagcaat ggacaagata tcggttgttg
     1081 ttccctgcta cagggaagaa gatgcgctgc cgcatttctg gaatgctcta aaaaaggtaa
     1141 tgacggaaga atttaaggat gtagagtttg aagtattgtt tgttgacgac ggatcctctg
     1201 acggaacatt gggtattcta aaaaagctgg cagaagagga ttccagggta agatatatat
     1261 cattttcaag gaactttggc aaagagtccg ccatgtacgc aggtcttgaa aattcaaccg
     1321 gggattatgt ggttgtcatg gatgcggaca tgcaggatcc tcccgaactt ttatccgaaa
     1381 tgtacagaac agtgaagcac gatggctacg actgtgcagc gacaagaagg gtaaacagaa
     1441 taggcgaacc aaagataagg tctttctgcg cgagactgtt ttacaaactt atgagaaaaa
     1501 ttacgaagat tgacattgtc gacggtgcca gggattaccg ccttatgtcc agacagatgg
     1561 tggacgcaat tattagtgtg ggggagtata acagattttc aaaaggaatc ttcagctggg
     1621 ttggtttcaa caccaaatgg ctggagtatg aaaatgtaaa aagggtggac ggagagacca
     1681 agtggtcatt ctggaagctt ttgctgtatt catttgaagg catagttgcc ttttccaccg
     1741 tacctcttgc aatttcctca ataataggca ttattctttg tcttgtttca tttgctgcaa
     1801 ttgtatttgt tgtaataagg cagctggttt ggcataattc tgcctatggc tgggcttcaa
     1861 tggtaagcat aatccttatg cttagtgg
//
LOCUS       DAONVY010000465         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40818_length_1887_cov_3.633188, whole genome shotgun sequence.
ACCESSION   DAONVY010000465 DAONVY010000000
VERSION     DAONVY010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40818_length_1887_cov_3.633188"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PLI11_10285"
     CDS             complement(<1..1404)
                     /locus_tag="PLI11_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ53296.1"
                     /translation="MRRIFSFLKPYKKQLVVGPSFKLLEAILELLIPTLMVSIVDIGV
                     QNKDTSYILKMGGLMLGIAAVGLLSALICQYSASIASQGFGTDVRNALFKHIMSLSSK
                     ELDEIGTTGYTTRLTNDINTLQLAVAMLIRLVIRAPFISLGSIVMALYINARLSIIII
                     LTLPILVTIVYFIMKKTIPLYKKVMATLDSLTLVVKENLQGVRVIRAFARRDREKKRF
                     DNLNTDFANMSVKAGRISTLLNPATTLIMNIAIILILYFSGISIKLGNMTQGEIIAFI
                     NYISYMVTALLVTANLVILFTRAYTSAQRVSEVFEQSSSITDGGTKEIDKDAEYAVEF
                     DDVFFAYNEGFYVLTDISFKLKRGESLGIIGGTGSGKTTLINLMMRMYDADKGSIRIL
                     GKDIKSYNIDALRKSVALVAQKSVIFSGTIEENIRQGNMDATMEDIHEAAKNAEALEF
                     IMKKDDKFSHEIERGGTN"
     gene            complement(1404..>1887)
                     /locus_tag="PLI11_10290"
     CDS             complement(1404..>1887)
                     /locus_tag="PLI11_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPZ53297.1"
                     /translation="GPFLEENIEELWTYYCCGQVTVSNRFLAFPSSRNRLLGLILFKY
                     DIKGFLQWGLNFYNSWLSIEEINPFTNSTGGGWVPGGDTFVLYPNDDGKPLSSIRFEV
                     FREALQDMRALKALSEKKGKDKVLEIINNSIKGKLSYKTTDIKAEDLIGIRERVNMEL
                     "
BASE COUNT          489 a          411 c          366 g          621 t
ORIGIN      
        1 gttggttccg cctctttcaa tctcatggct gaacttgtcg tctttcttca taatgaactc
       61 aagagcttca gcattttttg cagcttcatg tatatcctcc atggttgcat ccatgtttcc
      121 ctggcgtata ttctcctcta tggttccgct gaatatgacg gatttttggg caacaagcgc
      181 aaccgacttt ctaagtgcat caatattata gcttttaatg tctttaccta atattcttat
      241 gcttccttta tccgcatcgt acatgcgcat cattaggttt atgagggtgg tttttccgct
      301 tcccgtaccg ccaataatgc caaggctttc tcctcgtttt agtttgaagg atatgtctgt
      361 aaggacataa aatccttcat tgtaggcgaa gaatacatca tcgaactcca cagcatattc
      421 tgcatccttg tcaatttcct tggttcctcc atcggttatt gaagagcttt gctcgaacac
      481 ctcacttacc ctctgagctg atgtgtaagc tcttgtaaag aggatgacaa ggtttgcagt
      541 aaccaaaaga gctgttacca tgtaagagat gtagttgatg aatgcaatta tttcaccctg
      601 agtcatgttc cccagtttta tgctgatgcc ggagaaatag aggataagta ttattgcaat
      661 attcataatc agggtggtgg caggatttaa aagggtagag attcttcctg ccttgaccga
      721 catgtttgca aaatccgtgt taaggttgtc aaagcgtttt ttctccctgt cccttcttgc
      781 aaaagccctt atcactctta caccttgcag gttttccttg actactagtg tcagtgaatc
      841 aagcgtcgcc atcacctttt tgtacagggg tattgtcttt ttcataatga agtagacaat
      901 agttacaagt atgggcaggg taagaattat aatgattgag agtctggcat ttatgtacaa
      961 tgccatgaca atgcttccta gtgatatgaa gggcgctcta attaccagcc ttatcagcat
     1021 tgctactgca agctgcaaag tgttaatgtc gtttgtcagt cttgttgtgt atcctgttgt
     1081 cccaatttca tcaagttcct tggatgaaag ggacatgata tgtttgaaca gggcatttct
     1141 tacatccgtt ccaaagccct gggaggctat ggaagcggaa tactgacaaa tgagcgcact
     1201 taaaaggcct actgctgcaa ttcccagcat gaggcctccc attttcaata tgtagcttgt
     1261 gtccttgttt tgaactccta tatctacaat actaaccata agggttggaa tcaaaagttc
     1321 aagaattgct tccaatagct tgaatgaagg accaactaca agctgttttt tgtacggttt
     1381 caaaaatgaa aatatccttc tcattacaac tccatattta ctctttctct tattccaatc
     1441 aaatcttctg cttttatatc cgtagtcttg tacgataatt taccttttat actattgttt
     1501 attatttcca agactttgtc cttgcctttt ttttcagaca aagcctttag tgcacgcata
     1561 tcctggaggg cttctctaaa gacttcaaac ctgatagatg acaatggttt gccatcatca
     1621 tttggataga gaacgaaagt gtctcctcct ggaacccatc ctcctccggt gctgtttgta
     1681 aaggggttga tttcctcaat tgacagccag gagttgtaga agtttagtcc ccactgcaaa
     1741 aaccccttaa tgtcatactt gaacagtatc aatccaagca atctgttcct tgatgaaggg
     1801 aaggcgagaa acctgtttga cacggtaacc tgtccgcagc agtaataggt ccagagttct
     1861 tcaatgtttt cctcaagaaa cggcccc
//
LOCUS       DAONVY010000466         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_40906_length_1884_cov_3.033352, whole genome shotgun sequence.
ACCESSION   DAONVY010000466 DAONVY010000000
VERSION     DAONVY010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40906_length_1884_cov_3.033352"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLI11_10295"
     CDS             <1..757
                     /locus_tag="PLI11_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53298.1"
                     /translation="MKLCKKYESLAVAKSIKSESKLKRIAVISVVVFVLAVIAIAVFF
                     RNMSGDYPNLVLYRKDDGIYMTNFRKGKTTKLDAQISAHGTRISEDGKRFFYMDDNDQ
                     SLHYMDLPYGLRSGVVDVSVRNFMINRTGSVVYCIKGNTSKGYLYKSNLKTSKLVAQG
                     VQDFRISPDGIKMICITDSQVMLYKNDNLEFSAMLSANSARPVLADLNLDHVYYIKDE
                     KLYMYNKGKVEEIFDGAKELVSCLEDGRGYFWK"
     gene            748..1032
                     /locus_tag="PLI11_10300"
     CDS             748..1032
                     /locus_tag="PLI11_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53299.1"
                     /translation="MEVKEVSLVAGEILYVDPVMPVVVYVSSGRISFVYEGVANTREE
                     TNEMYIKALSNVCLTSAGSLILFDQENQKWEKMEDNVSDFGITTKGEIYK"
     assembly_gap    1121..1130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1131..1429
                     /locus_tag="PLI11_10305"
     CDS             <1131..1429
                     /locus_tag="PLI11_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53300.1"
                     /translation="QTEEYERSMFIRKNTGSVENITHYYTSFFYYNPKNIFYTRSSPD
                     GKTTALYHFDEKEAKVFEEGVNSIVPLGGEYYGFYDFGASTRYGLNFYNIFGEK"
BASE COUNT          633 a          313 c          416 g          512 t
ORIGIN      
        1 catgaagcta tgcaaaaaat acgaaagtct ggctgtggca aaaagcataa aaagtgagtc
       61 aaaactaaaa cgtattgcag ttatatctgt tgttgttttt gtgctggccg taattgcaat
      121 agccgttttt ttcaggaaca tgtctggtga ttacccaaac cttgttttat acagaaaaga
      181 tgacgggata tacatgacca actttcgaaa aggcaaaacc accaagctgg atgcacaaat
      241 atctgcccat ggtacaagaa tcagtgagga cggcaaaaga ttcttctata tggatgacaa
      301 tgatcaatcc ctgcactaca tggatttgcc ctatggcttg cgatcgggag tggtggatgt
      361 aagtgtcaga aacttcatga taaacagaac tggaagtgtc gtctactgca taaagggaaa
      421 caccagcaaa gggtatctat acaaaagcaa cttaaaaact tcaaaacttg tagctcaagg
      481 tgttcaggat ttcagaatat ctcctgatgg cataaagatg atttgcatta ccgactctca
      541 ggttatgttg tacaaaaatg acaacctgga gttttccgcc atgctttctg ccaattctgc
      601 cagaccggtt cttgcagacc tcaacttgga tcatgtgtat tacataaagg atgaaaagct
      661 gtatatgtac aacaagggca aggtggaaga gatttttgat ggtgcaaagg agcttgtaag
      721 ctgtcttgag gacggaagag gatatttttg gaagtaaaag aggttagcct tgtggcaggt
      781 gagattcttt atgtggatcc agtcatgccg gtagtggttt atgtttcatc aggaagaatt
      841 tcttttgtat acgaaggtgt tgcaaatacc cgtgaagaga caaacgaaat gtatataaaa
      901 gccctttcca atgtttgttt gacttccgct ggttccttga tactttttga tcaggagaat
      961 cagaagtggg agaaaatgga agataatgta agtgattttg gaattacaac aaagggtgag
     1021 atatataaat aaaggccgga gatgaaaaga ttggaactct ttatgttgat ggagaaaagc
     1081 tggctgacaa tatctacaaa gacagggtat atgccgactt nnnnnnnnnn accaaaccga
     1141 ggaatatgaa aggtccatgt ttatacggaa gaatactggt tcagtcgaaa atataactca
     1201 ctactacaca tctttctttt actacaaccc caagaacata ttctatacac gaagcagccc
     1261 ggacggcaaa accacggcct tgtatcattt tgatgaaaag gaggcaaagg tctttgagga
     1321 gggtgtgaat tccatagttc ccttgggagg agagtattac ggattctatg attttggagc
     1381 cagtaccaga tacggtctca acttctacaa tatttttggt gaaaagtaaa aacagattac
     1441 acaggaggta aaagaagatt gacaaaaaga acacgtagtc gcaaaactga tgtcaaggag
     1501 tacagcattt caccagatgg cagcaaaatc ctctacttta cagaggatag gtctgcttgt
     1561 acaaggacgg cgaattgcaa tttgaaaaag agtaccagta ttccgataaa accatgatat
     1621 gcttactact actattacat ccctaaacca tattttacct aagccagaat gatctgcatc
     1681 tgtacaatgg tacaatctca caagtatttg agaaggatgt ttcatccact gtacagacat
     1741 ataatttaat tggaaagatt gaggatgaat aaaagaggct tgaagcctct ttattttgtg
     1801 ttataggaaa ttgctctata tcggcataag gaagaaaaaa gaaaaatgta aaatatatac
     1861 attaaggggt gagttttgac taga
//
LOCUS       DAONVY010000467         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_41228_length_1873_cov_3.433443, whole genome shotgun sequence.
ACCESSION   DAONVY010000467 DAONVY010000000
VERSION     DAONVY010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41228_length_1873_cov_3.433443"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PLI11_10310"
     CDS             complement(<1..1404)
                     /locus_tag="PLI11_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53301.1"
                     /translation="MKARRGICSIVILMALIIGLLAMPSSSSVDYLPELDFINPYETR
                     ENASALIVEKVNNIRALHGLAPLGESNILVKSAKYKSLYISQYPENINETEFVSGPLK
                     DKSLNDILPYVEKEEILYMLSGGNTSDESLATYFSRTLETSFSSGRILLNPGIKSIGV
                     SIVQFQTMTGAYTVCVLHASSEESLQDEINVVPDNIVKNDTTPPEVEVMDTYIAKDYV
                     LSEEKILRNIKYMDNSKGACTITFDMDEINISKIGEYNLTIVVSDASGNKTEKIMKVH
                     VVPHVNPIISGDEIIIPIVSFDDVYDISKYVVVRDNYGIEYIHTEPKLAVRQDDGQEV
                     KVSVMNKAGLSIEKTMVLKFSDVEHFSSPVARVYPIYPAKGEPISIEMIKSAIVCRDE
                     EASIEVDTDSFNAIDLEVPGIYYCLANIKLFVDDAYVQTPVAVPVHVMLDDVFLSDKS
                     TKQQYDSNIEISYSKLNR"
     gene            complement(1485..>1873)
                     /locus_tag="PLI11_10315"
     CDS             complement(1485..>1873)
                     /locus_tag="PLI11_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPZ53302.1"
                     /translation="QGVKNYCVDVLKKGDDIVFLRKLKRGGADGSYGIEVAQLAGIPQ
                     AVTLRAKELLAILEEQDISRKEIRRIKRRNKEGEGQVDLVTYAANTVMRDEIVEELKN
                     LDVQTITPIEALNVLFNLHQKAMKRK"
BASE COUNT          502 a          428 c          317 g          626 t
ORIGIN      
        1 tctgtttaat ttcgaatagg atatctcaat attgctgtcg tattgctgct ttgtgctctt
       61 gtccgacaaa aagacatcat ccagcattac atgcacaggc acagctacag gagtctgcac
      121 atatgcatcg tcaacaaaca gtttgatatt ggcaagacag tagtatattc ccggaacttc
      181 caggtctatt gcgttaaaac tatctgtatc cacttctatg cttgcttcct catccctgca
      241 aactattgct gattttatca tttcaatgct tattggttca ccttttgcag ggtatatggg
      301 atagactctt gcaacaggcg aggaaaaatg ctcaacatcg gaaaacttga ggaccattgt
      361 cttttcgatg gaaagtcctg ccttgttcat aacagagacc tttacctcct ggccgtcatc
      421 ctgccttact gcaagttttg gctccgtatg aatgtattca atgccataat tgtcccttac
      481 caccacatat tttgatatat cgtacacatc atcaaatgat acaattggta tgattatttc
      541 atcaccggag ataatcggat tgacatgggg tactacatgc accttcatga ttttctcggt
      601 cttgtttccc gaggcatcgc tgacaacaat agtcaggttg tactccccta tttttgatat
      661 gttaatttca tccatgtcaa aggttatggt acatgcgccc ttgctgttgt ccatatattt
      721 aatgtttctc aatatttttt cttcactcag cacataatct tttgctatgt aagtatccat
      781 tacttccact tcaggaggtg ttgtatcatt ttttacaata ttgtccggca cgacatttat
      841 ttcatcctgc aaagattctt cactcgatgc atgcaaaacg cataccgtat aggctcctgt
      901 catggtttga aattggacaa tgctgactcc tatcgattta atacctggat ttaacaaaat
      961 tcttccactg gaaaaactgg tttccaaagt ccttgaaaaa taagtggcaa ggctttcatc
     1021 cgaagtatta ccaccactaa gcatatacaa tatttcttct ttttccacat aaggcaatat
     1081 atcgttcaaa cttttgtctt tgagaggtcc tgaaacaaat tctgtttcat taatgttttc
     1141 aggatactgg gatatgtata aagatttgta ctttgcactt ttgaccagta tattggattc
     1201 accaagaggc gcaagtccat gcaaagccct aatattgttc actttttcaa caatcagggc
     1261 tgatgcattt tctcttgttt cgtatggatt aatgaagtcc agttcaggaa gatagtcaac
     1321 actggaagat gaaggcattg caagaaggcc tataatcaag gccattaata ttactatgct
     1381 gcatatacct cttcttgctt tcataccatt aaccccttgt catttgtaaa aactcacgaa
     1441 tattatagca taagcggaat gcatattcaa taaacaagct ttgcctattt cctcttcatc
     1501 gccttctggt gaagattgaa taaaacattc aaagcctcta taggtgttat cgtctgaaca
     1561 tcgagattct tgagttcttc cacaatttca tcccgcatga cggtgtttgc ggcataggta
     1621 accaggtcaa cctgaccttc gccttccttg ttcctcctct ttattctcct tatttccttc
     1681 ctgcttatat cctgttcctc aagaattgca aggagctcct ttgctcttaa tgtaacagcc
     1741 tgcggaatac cggccaactg ggctacctca attccatagc tgccatcggc ccctccccgc
     1801 ttcagttttc taaggaaaac aatgtcgtct ccctttttaa gaacatcaac gcagtagttt
     1861 ttaacaccct gta
//
LOCUS       DAONVY010000468         1870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_41315_length_1870_cov_3.161983, whole genome shotgun sequence.
ACCESSION   DAONVY010000468 DAONVY010000000
VERSION     DAONVY010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41315_length_1870_cov_3.161983"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLI11_10320"
     CDS             <1..586
                     /locus_tag="PLI11_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPZ53303.1"
                     /translation="VMMFVGGVSFYNFALLGVCGVGVVALAFLKFAHVGRRIFAFLDP
                     FSVASDEGYQVVQSLYAIGSGGLFGRGLGQSVQKYLYLPEPYNDFIFSILAEELGFFG
                     VFIVLFLFGVFIWRGYKIATCANDLLGTLLATGITTIVAVQTIMNVAVVTSSMPATGI
                     SLPFFSYGGTSLLILMASMGILLNISKNSKYVKF"
     gene            complement(549..947)
                     /locus_tag="PLI11_10325"
     CDS             complement(549..947)
                     /locus_tag="PLI11_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPZ53304.1"
                     /translation="MVKYCISGGNSLHGTVDIYGAKNAILPILAATILNKGSTTLRNC
                     PNLLDVNITLDILRELGCRVEHSFDTVHVDSSSITTTNVPAEQTGKLRSSMVFLGALL
                     SRYKEVSISHPGECVTLLIKILHILNSWIY"
     gene            1085..>1870
                     /locus_tag="PLI11_10330"
     CDS             1085..>1870
                     /locus_tag="PLI11_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HPZ53305.1"
                     /translation="MKKLLVVDGNSIINRTYYATETSFMKTAEGLYTGAVYGFLNIFL
                     KYLEEDNPDYVAVCFDVKHPTFRHEEYEEYKATRKGMPDELAAQMPLLKELLHAFNVA
                     VVEKPGFEADDLIGTFCERASQKGVGCIVLTGDRDSLQLVNEKVVVKLPVTRGGKTNT
                     EFYDVDAVYEKYGVTPAQLIDVKALMGDKSDNVPGIPGVGEKTALSLIKEFGSLEGIY
                     ENIDKVARPALKKKLEEGKDIAYLSRKLVTIMRDVPLEITEEDA"
BASE COUNT          510 a          344 c          447 g          569 t
ORIGIN      
        1 tgtcatgatg ttcgtaggtg gtgtgtcctt ttacaacttt gcccttcttg gagtttgcgg
       61 tgttggggtt gttgcacttg cgttcctgaa gtttgcccac gttggaaggc gaatatttgc
      121 ttttcttgat ccgttttcag ttgcatccga tgaaggttac caagttgtcc agtccctgta
      181 tgcaataggt tcaggtggtc ttttcggaag aggtcttgga cagagcgtac agaaatatct
      241 ttatcttccc gaaccttaca atgactttat attctccatt ttggccgaag agctgggctt
      301 tttcggcgtg ttcatagttt tgttcctgtt tggagtattt atttggaggg gctacaagat
      361 agcgacgtgc gcaaacgatc ttttgggtac actgctggct accggaatca caactattgt
      421 agcagtccag acgattatga atgtggctgt tgtaacgtca tcaatgcccg caacaggaat
      481 atcgctccca tttttcagct atggaggaac ctcgctgctc atccttatgg cttcaatggg
      541 tatccttctc aatatatcca agaattcaaa atatgtaaaa ttttaatcaa tagggtaacg
      601 cactctcccg ggtgagatat agaaacttcc ttgtatctag aaagaagtgc tccaaggaaa
      661 accattgaag atcttagttt gcctgtctgt tctgcaggaa cattcgttgt tgttatactg
      721 cttgaatcaa catgtactgt atcaaagctg tgctcaaccc tgcatcccag ctctcttaaa
      781 atgtccaggg taatgttgac atccaaaaga tttggacagt ttctgagtgt cgtgctccct
      841 ttattcagta ttgttgcagc aagtatgggc agaattgcgt tttttgcacc gtatatatcg
      901 actgtgccgt gcaagctgtt gccaccggaa atacaatatt taaccatatg ttctactcct
      961 gtaattcatt gacacatata taatatgcat gatgtcctta actggtcaag gttgattgtc
     1021 caggttgtga catgggcaat attttgatat aatactaaaa acaaacatga ttggagttaa
     1081 atttatgaaa aaacttttgg ttgtggacgg taacagtatt attaaccgaa catactatgc
     1141 aacagaaact agttttatga aaactgcaga gggactgtat acaggcgctg tatacggttt
     1201 tttaaatata ttccttaaat atctggaaga ggacaatccg gattatgttg cggtttgttt
     1261 tgatgtaaaa catcccactt tcagacatga agaatacgaa gaatacaaag cgacgagaaa
     1321 aggcatgccg gatgaactgg ctgcccagat gccgctattg aaggagcttc tacatgcctt
     1381 taatgtggct gttgtagaga aaccgggttt tgaggctgat gacttgattg gcaccttctg
     1441 tgaaagagcc tctcaaaagg gtgtaggctg cattgtttta acaggagata gggattcttt
     1501 gcagctggtt aatgagaaag tagttgtcaa actgcctgta acaaggggcg gaaagacaaa
     1561 taccgagttt tacgatgttg atgctgttta tgagaagtat ggagtgacac cagctcagct
     1621 gattgacgta aaagccctta tgggcgacaa gtcggacaat gtgccgggca tacccggtgt
     1681 tggtgaaaag accgctcttt ccttgattaa ggagtttggc tcattggaag ggatatatga
     1741 gaatatcgac aaggttgcaa ggcctgcgct gaagaaaaag cttgaggaag gcaaggatat
     1801 tgcttatctt agcaggaagc ttgttacaat aatgagggat gtgccacttg aaatcactga
     1861 agaggatgca
//
LOCUS       DAONVY010000469         1861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_41572_length_1861_cov_4.115725, whole genome shotgun sequence.
ACCESSION   DAONVY010000469 DAONVY010000000
VERSION     DAONVY010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41572_length_1861_cov_4.115725"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1861)
                     /gene="pheT"
                     /locus_tag="PLI11_10335"
     CDS             complement(162..>1861)
                     /gene="pheT"
                     /locus_tag="PLI11_10335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPZ53306.1"
                     /translation="TDVKIGPSPKWMRDRLQHAGIRAINNIVDITNYVMLEFGHPMHA
                     FDRRFVRGDEIIVRRAKNGETMMTLDGIERFFDSSTLVIADKEGPIAVAGVMGGENSG
                     VMDDTKEVIFEAALFEPVSIRMAAKKLGMRTESSSRFEKGLNAITCAMAMERAAQLVE
                     LLGAGKVLKGTVDCYPVPYEERKVPFNVERINGLLGTEIPRERMAEILTSLEFKVFDD
                     YVVPPHFRPDIVCGADLSEEIARFYGYNNIDTRLLTGCETTLGRKNRRQTISDMIRNS
                     AKGLGYYEALTFSFESPSVFDMLNLPNDSPLRNAVVISNPLGEDFSVMRTSMAPSMLK
                     ILSLNHAKRNESVSVFEISYVYEKKEDVNELPNHKELLSLAAYGDMSFFDIKGDLEAL
                     LDSLKIKDYSFAACKDQPYMHPGMCAKVFINGREAGLIGKVHPKVCENFECPENTYLA
                     FVELEPVIEAAILIPTYKELPKFPAVARDLAIVVDKEVPANDVEKVILQRGGKVLEEL
                     VLFDYYDGPQVPKGKKSLAYSLSFRDNERTLTDEDVNKVMNKILNGLNTILSAEIRN"
BASE COUNT          519 a          467 c          306 g          569 t
ORIGIN      
        1 aatcactcct aagaagtatg ccttcatatg ctcccggaac ccaaatctca tcttcggaaa
       61 ggctcatgtc tcccgcatca ggcagggaga cacaaccatc cttaacatat cctttttcat
      121 ccgtgttctg cttcaaaaac tcatatatcg atttcatttt actaattcct tatctcagca
      181 ctcaatatgg tgttaagtcc gttgagtatc ttgttcataa ccttgttgac gtcctcgtca
      241 gtcagagtcc tttcattatc tctaaatgat agagaatatg caaggctctt ctttcctttt
      301 ggaacctgcg gtccgtcata atagtcaaac agaacaagct cttcaagaac ctttcctcct
      361 ctttgcaata taactttttc aacatcgttt gcaggcactt ccttgtcaac cacaatcgca
      421 aggtcccttg ctactgcagg gaacttggga agttccttgt aagttggaat aagaatcgcc
      481 gcctctatta caggctccag ttctacaaat gcaagatatg tgttctcggg acattcaaaa
      541 ttctcacata cctttggatg caccttgcct atcaagcctg cttccctacc gttaataaat
      601 acttttgcac acatgcctgg atgcatgtat ggctgatcct tacatgctgc aaaagaataa
      661 tccttaatct tcaaagaatc cagcaaagcc tccaaatctc cctttatgtc aaagaaagac
      721 atgtcaccat aagctgcaag acttaaaagt tccttgtgat ttggaagctc gttaacatct
      781 tccttcttct cataaacata ggatatctca aacacgctta cggattcatt gcgctttgca
      841 tgattcaaac tcaaaatctt taacatgctc ggagccatcg aggtcctcat aacgctgaaa
      901 tcttctccaa gaggattgga aatgacaaca gcatttctca aaggactgtc atttggcaaa
      961 ttcaacatgt caaaaacaga aggactttca aaagagaagg ttagagcttc gtaataacca
     1021 agaccttttg cagaatttct tatcatgtct gaaatcgtct gtcttctgtt ctttcttccc
     1081 aaagttgttt cacatccagt aagcagcctg gtatctatat tattgtatcc atagaatctg
     1141 gctatttctt cggaaagatc cgctccacaa acaatatccg gtctgaagtg aggcggtacc
     1201 acatagtcgt caaacacttt gaactcaaga cttgttagaa tctccgccat tctttccctg
     1261 ggtatttccg tacccaataa tccatttatt ctctctacat taaatggaac ctttctctcc
     1321 tcatatggaa cggggtagca gtctacagta cctttaagaa ctttgccagc acctaaaagt
     1381 tccacaagct gcgctgctct ttccatggcc attgcacaag ttattgcgtt tagtcccttt
     1441 tcaaaccttg aagaggattc cgttctcatg ccaagttttt tagcagccat tctgatagaa
     1501 acaggttcga acaacgctgc ctcaaaaata acttccttag tatcatccat aacacctgag
     1561 ttctctccgc ccatgacacc agcaactgca atagggcctt ccttgtctgc aattaccaga
     1621 gtggatgaat caaaaaacct ttcaataccg tcaagagtca tcatagtctc accgtttttt
     1681 gcacggcgca ctattatctc atcacctctt acaaatcttc tgtcaaatgc atgcatgggg
     1741 tgtccaaact caagcatgac ataatttgtg atgtcgacta tattgttaat agctcttatg
     1801 cctgcatgct gcagtctgtc cctcatccat ttgggagatg gtccaatctt tacatcagta
     1861 a
//
LOCUS       DAONVY010000470         1852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_41841_length_1852_cov_3.058987, whole genome shotgun sequence.
ACCESSION   DAONVY010000470 DAONVY010000000
VERSION     DAONVY010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41841_length_1852_cov_3.058987"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLI11_10340"
     CDS             <1..230
                     /locus_tag="PLI11_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPZ53307.1"
                     /translation="SRGYNGGYMLAKDPSEYRVIDILEITEGNLACVSCLEDEVNECK
                     RAGFCKSLKVWQGLDNVIKNYLSSITLQDLI"
     gene            297..1031
                     /locus_tag="PLI11_10345"
     CDS             297..1031
                     /locus_tag="PLI11_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53308.1"
                     /translation="MYFFSFSKLDEILKKEEKAYCQTYFRKVLSENRNLAIKLVNDTR
                     ITFPCFFVLMPVVEKENIYSLLREDLKKAYDFSKSVSANKPSNLKKDIKNKILLKWMV
                     ESSHLHNNLGGDFNEVVDVAVSILANEYRELSLLGIISELIFMRNRYDENIHDLVWAY
                     FRFNTPITLKYIARFLDSKNPKDRKLAKKLLGCEAKSCKDFLKYMTEDESKMIYTDES
                     FQYASNPRVYAAKEGTDHDLHKGKQV"
     gene            1003..1797
                     /locus_tag="PLI11_10350"
     CDS             1003..1797
                     /locus_tag="PLI11_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine gamma-glutamyltransferase"
                     /protein_id="HPZ53309.1"
                     /translation="MIYIKGSKFDITELKSTYPVQSVEHKVLVKMEESSFRYNFKSED
                     VLKFELLLRKEIAASARALDRSGFGFAVFSKSQCNPEYWNRENNGGFSLKQGKSPSKA
                     IRNIFENGRLYRNECATAMVIVYYGALLSVFKDRFDETFPYIYLMDWYIIDPLLQGIG
                     YPRKAHDMLIGDRAYFKNPDVHPETMWLQGENVIVLAEDLYYGHGIGIANAETFIEIL
                     NRNRKEGAAKSAYLMDEVARPDFELLSDVYHERIAVAQTIYWRNIV"
BASE COUNT          595 a          339 c          411 g          507 t
ORIGIN      
        1 caagcagggg gtacaatggc ggatacatgc ttgcaaaaga cccttcagaa tacagagtga
       61 ttgacatatt ggaaataacg gagggaaatc tggcttgtgt ttcctgtctt gaggacgagg
      121 taaacgaatg taaaagagca ggcttctgca aatccttgaa agtatggcag gggctagaca
      181 atgttataaa aaactacctg agcagcataa cccttcagga tcttatctaa gtaatacatt
      241 atcggccagc aaaagaaatg catacaatat accaggaagt ttaaatggtg tattgtatgt
      301 atttttttag tttttccaaa ctggatgaaa ttcttaaaaa ggaagaaaaa gcttactgcc
      361 agacgtattt caggaaagtg ctttctgaaa acagaaacct tgcaataaaa cttgttaatg
      421 atacaaggat tacttttccc tgcttttttg tccttatgcc tgttgttgaa aaggaaaata
      481 tttattctct gttgagagaa gacttgaaaa aggcttacga tttttcaaaa agcgtttcag
      541 ccaataaacc ttcaaaccta aaaaaagata ttaagaataa aatcttgctt aaatggatgg
      601 ttgagagcag ccacctccat aacaacctgg gcggggattt caacgaagtg gtcgatgtcg
      661 ccgtgtccat cttggcaaac gagtaccggg agctgtcgct gctcggtata atttcggaac
      721 ttattttcat gcgaaacaga tatgacgaaa acatccatga tcttgtctgg gcatatttca
      781 gattcaacac ccccatcact ctgaaataca ttgcaagatt tcttgattca aagaatccca
      841 aggacagaaa acttgctaaa aagcttttag gctgtgaagc aaagtcctgt aaagactttc
      901 ttaaatatat gactgaagac gaatcaaaga tgatttatac ggacgagagt tttcagtacg
      961 catcaaaccc acgagtatat gcagcaaagg aaggtacaga ccatgattta cataaaggga
     1021 agcaagtttg acataaccga gctaaaaagc acttacccgg tacaaagtgt tgaacataag
     1081 gttcttgtca aaatggagga aagttctttt aggtataact tcaagagtga ggatgtttta
     1141 aagtttgaac ttcttttaag aaaagaaatt gctgcctctg caagagcgct tgatagaagc
     1201 ggttttgggt ttgccgtctt cagcaaatcc cagtgcaatc cagagtactg gaaccgggaa
     1261 aacaatggcg gcttttcttt gaaacaaggc aaaagtcctt cgaaggctat acgcaacata
     1321 tttgaaaacg gcaggcttta cagaaacgag tgcgccacag ccatggttat tgtctactac
     1381 ggagcccttt tgtccgtttt caaagacagg tttgatgaga cttttcccta catttacctt
     1441 atggattggt atataatcga tcctttgctg caggggattg gttaccccag aaaagcccac
     1501 gacatgctta ttggggatag ggcgtacttc aaaaatcccg acgttcatcc tgaaacgatg
     1561 tggctgcaag gtgaaaatgt gattgttctg gcagaggatc tgtactacgg tcacggcata
     1621 ggtattgcaa atgcagaaac ctttattgag atactgaaca gaaacagaaa ggaaggagct
     1681 gcaaaaagcg cctacctcat ggatgaagtg gcgcgtcctg atttcgaatt attgtcagat
     1741 gtatatcatg aaaggattgc cgtagcacaa acaatttact ggagaaatat agtttgacta
     1801 gtctctgccg tagatggagg gcagttttct tttatgctcg cttcttgagt ga
//
LOCUS       DAONVY010000471         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_42050_length_1846_cov_3.120603, whole genome shotgun sequence.
ACCESSION   DAONVY010000471 DAONVY010000000
VERSION     DAONVY010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42050_length_1846_cov_3.120603"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PLI11_10355"
     CDS             <1..1055
                     /locus_tag="PLI11_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53310.1"
                     /translation="AFPSRYDFFTPGNEANIGFYSANCSFEVRNIEVYNEPDLLFSNQ
                     YTLTFNDLALGELARGRYDNWFIEWPSSNISSVGESGDLKYLEVKGYFQTVFAYPVAE
                     KYTVDMKMLATNLSSNAGLFIRSSHDRFLANSKSAPNAEYFEADGAGSIGGTGLFLKP
                     LHDKNVMQIVIKQYDESAAKKIAAPAVTIDISSIMGENDQTTAITNIRFADEGSVIKA
                     YVNDTLIAAIELSSETITYPDFDQIANFGKSTETYVKTATVKDANGEVLLTINNTLVC
                     AEYSIMGFATRNQSQDHPLFIDDLNVEIDMVKLDVPQEPETEEPIPETGDVFALFPMI
                     TLLGLAAVPAKIRKKK"
     gene            complement(1112..>1846)
                     /locus_tag="PLI11_10360"
     CDS             complement(1112..>1846)
                     /locus_tag="PLI11_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53311.1"
                     /translation="ISNHDLFTDTSEYTDDRILMIPGVEYSRSEHMLTIGVDRSLHES
                     VHQTAIDITNEIGGFTVLCHPNWGRKGYWSHDKMMSLQGYKGIEVLNMLIYRLSGSGL
                     AADAWDYLLSNGRLVYGFGNDDFHAIPDAGRSFNVIFAKEPSFRALKESIDKGSFCAS
                     SGLYPEYLHLEDNIIRVKARYPIATYVDEFTYRFISENGKVLDVQFGKEGVYNLTGEK
                     YVRVEAIGENGAMLYFQPVFQESFLK"
BASE COUNT          562 a          407 c          351 g          526 t
ORIGIN      
        1 cagcattccc aagcagatat gacttcttta cccctggcaa cgaggcaaac ataggattct
       61 acagcgcaaa ctgctctttt gaagttagaa acattgaagt atataacgaa cctgatcttc
      121 tgttcagcaa tcagtacact ttgacattca atgatctggc actgggcgaa ctggcaagag
      181 gcagatatga caactggttt attgaatggc cttcaagcaa tatttcctcg gttggagaaa
      241 gcggcgacct gaaatatctt gaggttaaag gatacttcca gacagtattt gcttatcctg
      301 tggcggaaaa gtacacagtg gacatgaaaa tgcttgctac aaacctttca tcaaatgctg
      361 gtttgtttat aaggtcaagc catgacagat tccttgcaaa cagcaagagt gcacctaatg
      421 ccgaatactt tgaagctgat ggtgcaggct caattggcgg aactggtctt ttcctgaaac
      481 cattgcatga taaaaatgtt atgcaaatcg ttataaaaca atacgacgaa tctgccgcca
      541 agaaaatagc ggccccggct gtaacaatag atatctccag catcatggga gaaaacgacc
      601 agacaaccgc aattacaaat attcgttttg ctgacgaagg ttcagttata aaggcttatg
      661 taaatgacac cctgattgct gctattgagc tttcaagcga aacaattaca tatcctgatt
      721 tcgaccagat agcaaatttt ggcaagagca ccgaaacata tgtaaagaca gccacagtca
      781 aggacgccaa cggtgaagta ttgcttacca taaacaatac ccttgtttgt gctgaataca
      841 gcattatggg atttgctaca agaaatcaaa gccaggatca tcctcttttc atagatgact
      901 tgaatgtgga aatcgacatg gtaaaattgg atgttccaca ggaacctgaa acagaagagc
      961 caattcctga aacaggtgat gtgtttgctc ttttcccaat gattacatta cttggacttg
     1021 ctgcggttcc tgcaaagatt agaaagaaga aataattgct gactaactag aataaggccc
     1081 cgacattttg tcagggcctg atttttatac tctatttcaa aaaggactct tgaaaaactg
     1141 gctgaaagta tagcattgcg ccgttctcgc ctattgcctc cacccttaca tacttctcac
     1201 cagtcaagtt atacactcct tcttttccaa actgcacatc cagaactttt ccattctcgc
     1261 ttatgaatcg gtaagtaaac tcatccacat aagttgcaat gggatatctt gcctttactc
     1321 ttatgatgtt gtcctccaga tggagatact cgggatacaa accactggat gcacaaaagc
     1381 ttcccttgtc tatggattct tttaatgccc taaaacttgg ttcctttgca aaaattacgt
     1441 taaagcttct tcctgcatca gggattgcat gaaagtcatc atttccaaaa ccatatacca
     1501 gtctgccatt tgagagtaaa taatcccagg catctgctgc aaggcctgaa cctgacaagc
     1561 gataaatgag catgtttaaa acctcaatgc ccttgtagcc ttgcaggctc atcattttat
     1621 catgactcca gtaccctttt cttccccagt ttgggtgaca cagtaccgta aatcctccta
     1681 tttcgtttgt aatatctatt gcggtttgat gaacactttc atgcagggat ctatcaacac
     1741 caattgtgag catgtgctcg gatcttgaat attcaacacc aggaatcatt aaaatcctgt
     1801 catccgtata ctcgcttgta tctgtaaata aatcatggtt tgaaat
//
LOCUS       DAONVY010000472         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_42079_length_1845_cov_3.189385, whole genome shotgun sequence.
ACCESSION   DAONVY010000472 DAONVY010000000
VERSION     DAONVY010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42079_length_1845_cov_3.189385"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLI11_10365"
     CDS             complement(<1..191)
                     /locus_tag="PLI11_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53312.1"
                     /translation="MHRKTHVENKPKNAMKTLARMYKYMSGHTIGLVFAIILIIFSSL
                     AGIIGTSLLRPLINNLMNN"
     gene            complement(184..>1845)
                     /locus_tag="PLI11_10370"
     CDS             complement(184..>1845)
                     /locus_tag="PLI11_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ53313.1"
                     /translation="SVRANTSFTNDDTRYIITIGAIMILLALIALLCGAIAARLAAVA
                     AQGGGTELRYDLFKQIQTFSFADLDKFSVPSLITRLTTDITNIQMAGMMSLRMLIRAP
                     FMLLFALILALNINSELALVFLVAIPILGIALYFIITTAHPRFRKLQKKFDALNASIQ
                     ENLTGIRVVKSFVREDYEKEKFKKSNDDFTNTAIGAVKVVIFTMPLMQLVIYGCIVAI
                     LWFGSGMVADTTITRILTGAESASGMQAGDLISFISYVTQILISLMMISFLFLMFVRS
                     KASAERVFEVMDTESTIKDPENPVMEVKDGSIVFNNVSFRYEEGKGEDTLSGINLSIA
                     SGENIGIIGATGSAKSTLVQLIPRLYDVIQGEVLVGGVNVKEYSLKSLRDAVAMVLQK
                     NTLFSGTVRDNMKWGNEDATDEEIIEALKQAQAWPFVSKLEKGLDHWVEQGGTNFSGG
                     QKQRLCIARSLLKKPKVIILDDSTSAVDMATDSKIRASFKENLKGITVIIIAQRVRSI
                     SHCDRIIVMDDGKINAIGTHEELLENCEIYEDIYNSQQEGVLADA"
BASE COUNT          476 a          404 c          367 g          598 t
ORIGIN      
        1 atattattca tcaggttgtt tatgagtggt ctcagcagtg aagtgcctat aattcctgcc
       61 aaagagctga aaatgattaa gattattgca aatacaagcc ctattgtgtg tccggacatg
      121 tatttataca tgcgcgcaag agttttcata gcgtttttag gtttattctc tacatgtgtt
      181 tttctatgca tctgcaagca ctccttcctg ctgtgaattg tatatgtctt catatatctc
      241 gcaattttca agaagctcct catgtgttcc tattgcattt attttgccat catccatgac
      301 aattatcctg tcgcagtgag atatggatct tactctttgt gcaattatta tcacagtaat
      361 tcctttcagg ttctctttga aagaagcgcg tattttcgaa tccgttgcca tgtctacagc
      421 acttgtggag tcatccagta taattacctt tggttttttc aacaaggatc tggctataca
      481 gagtctttgt ttctgtcctc ctgaaaagtt tgtgccgcct tgttcaaccc agtggtcaag
      541 acctttttcc agcttgctta caaaaggcca tgcctgggct tgctttaatg cttcgattat
      601 ttcttcatct gtggcgtctt cattacccca tttcatattg tctcttacag ttcccgaaaa
      661 tagggtgttc ttctgcaaaa ccattgccac ggcatctctt aaacttttca aggagtactc
      721 ttttacattg actccaccta ccaaaacttc tccctggatt acgtcataca gccttggaat
      781 aagctggaca agagttgact ttgcagagcc agtggctccg attattccta tgttctcgcc
      841 agacgcaatg gaaagattaa tgcctgacaa tgtatcttcg ccttttcctt cctcatatct
      901 aaagctcaca ttattaaaaa caattgatcc atcttttact tccataacag gattctcagg
      961 atcttttatt gttgactcgg tatccattac ctcaaataca cgctccgctg atgctttcga
     1021 gcgtacaaac atgaggaaaa gaaaagatat catcatcagt gatatgagta tctgggtgac
     1081 ataggagata aaacttataa ggtcgcctgc ctgcatgcct gatgcgcttt ccgcaccggt
     1141 aagtattctg gtgatagtgg tgtctgccac catgccgctg ccaaaccaga ggattgcaac
     1201 tatacatccg taaattacaa gctgcatcaa gggcatggtg aatatgacca cctttacagc
     1261 tccgatggca gtattggtaa agtcgtcatt tgattttttg aacttttcct tctcataatc
     1321 ctcgcgcaca aaactcttta caacccttat gcctgtaagg ttttcctgga tggaggcatt
     1381 gagggcatcg aatttcttct gaagttttct gaaccttggg tgggcagttg tgattatgaa
     1441 ataaagggcg atgcccagta ttggaattgc gacaaggaat accaaggcaa gctcgctgtt
     1501 tatgtttaat gcaagtatta aagcgaaaag cagcataaag ggtgcccgta tcagcatcct
     1561 taaagacatc atgcctgcca tttgaatatt ggttatgtct gttgtaagtc ttgtaatcag
     1621 tgatggaaca ctgaatttgt ccaaatccgc aaaggaaaaa gtttgtatct gtttgaaaag
     1681 gtcatatcgg agttcggtgc ctcctccttg ggcggctacc gctgcaagac gggcggcaat
     1741 agctccgcac agcagtgcaa tcaatgccag gagtatcatt attgccccta tggttataat
     1801 atatcttgtg tcatcatttg tgaaacttgt gttcgccctt acaga
//
LOCUS       DAONVY010000473         1838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_42313_length_1838_cov_3.123388, whole genome shotgun sequence.
ACCESSION   DAONVY010000473 DAONVY010000000
VERSION     DAONVY010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42313_length_1838_cov_3.123388"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLI11_10375"
     CDS             <1..516
                     /locus_tag="PLI11_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPZ53314.1"
                     /translation="SYAVNPLLPEDIVVLESCLVSDDFHARYDAKSKTYKYLVHASPR
                     RNPLLNNRVWHVNRELDLALMQKAAMHIQGKREYDAFRAQGGYAKTTTRTVFHTTVKQ
                     EYLPNIYSIEVCGDGFLYNMVRIIAGTLVYVGLGKIAPDNIPFIIESKDRTKAGITAP
                     PHGLYLYDVSY"
     gene            complement(506..1690)
                     /locus_tag="PLI11_10380"
     CDS             complement(506..1690)
                     /locus_tag="PLI11_10380"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPZ53315.1"
                     /translation="MRMKKLTEEFIRMFGKSDRQIRFFAAPGRVNLIGEHIDYCGGYV
                     FPAALTLDNVVAVRENGTSKMNMCATDLARVYTADLSDIDKAKELKWGNYQAGMAKEL
                     LEAGINVRGVDMLYDSTLPFGAGLSSSASIEVVTGYALASLFANEMPNRIEIALMGKR
                     CENNFVGVNCGIMDQFASAMGKKDCAILLNCDTLEYEYVPLDLGDYVLILANTNVKHK
                     LGSSKYNERRQEVEKGLSYIHTAAPDDKRPYLCNYTVEDYLKYDEYITDDIIRKRVKH
                     VIYENERVLHATKVLKQGDLVSFGQILCEANDSIRYLYEVTGIELDTLFDLAMEEEGC
                     IGSRMTGAGFGGCTVNIVHKDKIESFKKNVGGKYTERTGLQADFYICRIGDGVREVSV
                     NN"
BASE COUNT          526 a          417 c          310 g          585 t
ORIGIN      
        1 tcttatgctg taaatcctct tcttccggag gatattgttg tacttgaatc atgccttgtt
       61 agcgacgatt ttcacgcaag atacgatgcg aaaagtaaaa cctacaagta tctggtgcat
      121 gcatctccaa gaagaaatcc acttttaaat aatagggtct ggcatgtaaa tagagagctt
      181 gatttggctt tgatgcaaaa agcggcaatg catattcaag gaaaaagaga gtacgatgcc
      241 ttcagggccc agggaggata tgcaaaaact accacccgta ctgtattcca cactacggtc
      301 aaacaggagt atcttcctaa tatatactcc attgaagtct gtggagatgg atttttatac
      361 aacatggtaa gaataattgc cggaactctt gtatatgttg gccttggaaa gattgcacct
      421 gacaacatac cttttattat tgaatcaaaa gacagaacaa aagcggggat aaccgccccg
      481 ccccatggtc tgtatttgta tgacgtcagt tattgacaga cacttccctg acaccatctc
      541 ctattctgca aatataaaag tctgcttgta atcctgttct ttctgtgtat ttcccgccca
      601 cattcttttt aaaactttca atcttatctt tgtggacaat attgacagtg cagccgccga
      661 aaccggctcc tgtcattctg cttcctatgc acccttcctc ttccattgcc aaatcaaata
      721 acgtatcaag ttcgatacct gtaacttcat acaaatatct gatggaatca ttagcttcgc
      781 acaagatttg tccaaaactt acaaggtctc cttgctttaa tacctttgtt gcgtgcaaaa
      841 ccctttcatt ctcatatatc acgtgtttta cacgcttcct tattatatca tctgttatat
      901 attcatcata ctttaagtaa tcttccacag tatagttgca taaatatgga cgtttgtcat
      961 caggagctgc agtgtgaata tacgaaagac ctttctccac ttcctgcctt ctttcattgt
     1021 acttggagct ccccaattta tgtttcacat tagtgttggc caatatcaac acataatcac
     1081 caaggtcaag aggcacgtat tcatattcca atgtatcgca attaagaaga attgcacaat
     1141 cctttttgcc catggcggaa gcaaactggt ccatgatacc gcagtttaca ccaacaaaat
     1201 tgttttcaca tctcttcccc attaacgcaa tctcaatacg attgggcatt tcatttgcaa
     1261 ataagctggc aagcgcatag cctgtgacca cttcaataga agctgatgag gacaagcccg
     1321 ctccaaaagg cagtgtgctg tcatatagca tgtccacgcc tcttacgttt ataccagctt
     1381 caagcagttc ttttgccatg cctgcctggt agttccccca tttcagctct ttggccttat
     1441 ctatatctga caaatcagca gtatatacac gcgcaagatc agttgcacac atattcattt
     1501 tgcttgtgcc gttttccctt acagcaacaa cattgtccag agtaagtgct gcaggaaata
     1561 cataaccacc gcaatagtcg atatgttcgc ctatcagatt aactcttccg ggcgcagcaa
     1621 aaaatcgaat ctgtctgtct gattttccaa acattctaat gaattcttct gttaatttct
     1681 tcatcctcat ttctccattt atcccctttt cattgttgtt gattttccga taataatagt
     1741 atataatgta atatggattt agtatatacc ttttaggtaa atatttactt gctaggagtt
     1801 tttaaaatga attactctat aggagacaga atagtgta
//
LOCUS       DAONVY010000474         1837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_42339_length_1837_cov_2.218294, whole genome shotgun sequence.
ACCESSION   DAONVY010000474 DAONVY010000000
VERSION     DAONVY010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42339_length_1837_cov_2.218294"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1217
                     /locus_tag="PLI11_10385"
     CDS             78..1217
                     /locus_tag="PLI11_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53316.1"
                     /translation="MGVRTVLLFVLSMVATLFLAGCGGHSVDESDGFNVTHEEFSNIL
                     SEKAKDKGVKMPKQNEDKSVLTREEMCQFISEYINNTPYLKSKYIPVEILVEQGEESR
                     FFKKMFQGIEAGSGKDDILLKQMENAKRMSIRTEVMKDGSIRTFYIYSEDASRKRFEN
                     ILKDYDLIDERYREACLDACHFGYIDIFFSDKSYEAYFNPKEEISREEVIKVIDNLFE
                     KEYLPLDCIKINYWEEDEFDNGLNQEHAYLCINDLPCLYAAKKLEKKLGENDKVYMTI
                     GFDNKSFSLLYSSMVFGMLELPVSYFLERNLVVEVTRLDSENIKAGLFVCSEVICDMY
                     RESFLEFANEVIANGDSNTRVFGDIKVDIYLSHHEIYVLTLGSDA"
     gene            1217..>1837
                     /locus_tag="PLI11_10390"
     CDS             1217..>1837
                     /locus_tag="PLI11_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53317.1"
                     /translation="MKRVLIVFLFWLLLSCSIRAVDDFSVYGDGVSVTFSDIRNTLPA
                     MYRNVPVIYVNTESGTKPYYFNVEMYIDKGFLVYGDPASCVELGYSNSFKQTPNGYFY
                     SSGGIRGEYRYLGLSINGTVYTNIAFPEDTVPGEHVYRIVKYASLPDYLKERYGIPKD
                     GNAYFEGIRHLIDSPDSPAWNFVHTYYDGRTVTVYEKFKESKMVGDG"
BASE COUNT          566 a          280 c          456 g          535 t
ORIGIN      
        1 tttgagtatt ttcgggtaat aatgcgacaa aatactacgg actgatataa tatccataaa
       61 aaacgaggag gttttttatg ggtgttagga cagtgctttt atttgttttg tccatggtgg
      121 ctaccctttt tttggcgggg tgtggtggac atagtgtgga tgaaagtgac ggttttaatg
      181 taactcatga ggaattttcc aatatccttt ctgaaaaggc aaaggacaaa ggtgtaaaaa
      241 tgccaaaaca gaatgaagac aagagtgttt taacaaggga ggagatgtgc cagttcatat
      301 cggagtatat aaacaatact ccttacttga agagcaaata catacctgta gaaatactgg
      361 tggaacaggg agaagagtcc agatttttca agaaaatgtt tcaaggaatt gaagctggaa
      421 gcgggaaaga cgatattttg ttaaaacaga tggaaaatgc aaaacggatg agcataagaa
      481 cggaagtgat gaaggatgga tctatacgca ccttctatat ttactccgag gatgccagca
      541 gaaaaagatt tgaaaacatc ctcaaggatt atgatttgat tgatgaaagg tatagggagg
      601 catgtcttga cgcctgccat ttcggatata ttgacatatt cttcagcgat aaaagttatg
      661 aggcgtattt caatcccaag gaagaaatat ccagggaaga agttattaag gtaattgaca
      721 atctgtttga aaaagagtat ttgccccttg actgcatcaa aataaattat tgggaagaag
      781 acgagtttga caacggcttg aaccaggagc atgcatatct ttgcattaac gacctgcctt
      841 gtctatatgc agcgaagaag ttggagaaga aacttggaga gaatgacaag gtatatatga
      901 caataggttt tgacaacaag tcattcagtt tgctgtacag ctccatggta tttgggatgt
      961 tggagcttcc tgtttcatac tttttagaaa gaaatctggt cgtggaagtg acgcgtcttg
     1021 attcggagaa tataaaggcg ggacttttcg tatgctcgga agttatatgt gacatgtaca
     1081 gggaaagttt cttggaattt gcaaatgagg ttattgcaaa cggcgacagc aacacaagag
     1141 tgtttggcga tatcaaggtg gatatttatc tttcccatca tgagatttat gtattaactt
     1201 tgggaagtga tgcataatga aaagggtctt gattgtcttt cttttctggc ttcttttgtc
     1261 ttgttccatt cgggcagtgg atgacttttc cgtatatggt gatggagtgt ctgtgacttt
     1321 cagtgatata cggaatacac tgcctgccat gtacaggaat gttcctgtaa tatatgtaaa
     1381 tacagaatcc gggacaaaac cgtattattt caatgtggag atgtatattg acaaggggtt
     1441 tcttgtttat ggagaccccg catcctgtgt tgaattgggc tattccaatt cgttcaagca
     1501 gacccctaat gggtattttt actcaagcgg cggaataagg ggtgaataca gatatcttgg
     1561 cctgtccata aatgggacgg tatatacaaa tattgcgttt cctgaggata ccgtaccggg
     1621 tgaacatgtg tacagaattg tgaaatacgc cagcttgccc gactatctga aagagagata
     1681 cggaatcccg aaggatggca acgcgtattt tgagggcata agacatctca ttgacagtcc
     1741 tgacagtcct gcctggaatt ttgtgcatac atattatgat ggaagaacag ttaccgtgta
     1801 tgaaaagttc aaggagtcaa aaatggtggg agatggg
//
LOCUS       DAONVY010000475         1833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_42456_length_1833_cov_2.069741, whole genome shotgun sequence.
ACCESSION   DAONVY010000475 DAONVY010000000
VERSION     DAONVY010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42456_length_1833_cov_2.069741"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLI11_10395"
     CDS             complement(<1..528)
                     /locus_tag="PLI11_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019420918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53318.1"
                     /translation="MKYVNPYENLTDGKWMKANFHTHAGTGPGTCGANPTDVVVNTYK
                     YLKYDVLCISNHDIFTDTSNYTDDRILMVPGVEYSRSEHMLTIGVDRSLHEKDHQTAI
                     DMANDMGGFTVLCHPNWGRKGYWSQEKMLNLKGYKGIEVLNMLIYRLSGSGLAADAWD
                     YLLSNCRLVYGFGNDD"
     gene            728..1030
                     /locus_tag="PLI11_10400"
     CDS             728..1030
                     /locus_tag="PLI11_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53319.1"
                     /translation="MKRITIFILLFALIFSLGVFAETITLTWDFEDATGDTHETSGIF
                     SESWLYKFRNPNEDVNKVIDKDGNKVYQVGALTQLVYKDKLEDPYTLSYRLQMGRL"
     gene            1037..1714
                     /locus_tag="PLI11_10405"
     CDS             1037..1714
                     /locus_tag="PLI11_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53320.1"
                     /translation="MNNGVFIRAAEMPEVDGKTVHFYDSDGIPPSGSWIYTSGLSIVF
                     KHLGSGDNPNPSLRLNFKSYDDNTEQYPMKVNNQYVDISLPEGTNLANFHDIKITDDG
                     TTIEIFFDNQKLATITMSDVGSYPDDIPHKDVEFYKKAVIKDANGNELANYDNVRLAK
                     SSVICFASRNAQPQNFSYNLDNVVLTIEKEDAPQNPETSDAISVMAFVILMAFISILI
                     KRRFAYN"
BASE COUNT          579 a          381 c          325 g          548 t
ORIGIN      
        1 atcatcattt ccaaaaccat ataccagtct gcagtttgag agtagataat cccaagcatc
       61 tgctgcaagg cctgaacctg acaagcgata aatgagcatg tttaatacct caataccttt
      121 gtagcctttc aggttcaaca ttttctcctg gctccagtag cctttcctcc cccagtttgg
      181 gtgacacaat actgtaaagc ctcccatatc gttggccata tctattgcgg tttgatgatc
      241 cttctcatgc agggatctgt caacaccaat tgtgagcatg tgctcggatc ttgagtattc
      301 aacgccagga accattaaaa tcctgtcatc ggtataattg cttgtatcag taaatatgtc
      361 atggtttgaa atgcataaca catcatattt caaatacttg tatgtattta caaccacatc
      421 ggtgggattg gctccgcatg tgccaggacc tgtgccggca tgggtgtgga aatttgcttt
      481 catccattta ccgtccgtca aattctcgta tggatttacg tatttcatat ctctatcagt
      541 cctttcgttt tcaacttaac aacacccata taatatacac atcatattgg tttgtccatg
      601 aggttttgaa aagtaaatat aaaaatacag taataatgtt gaaaatgcat attttcgttt
      661 agtttcttta tacatttact ctttttttaa tagtagaata aacaaaaaaa gaaagagggt
      721 tgaattaatg aaacgcatta ctatttttat ccttttattt gctttaattt tttcacttgg
      781 agtttttgct gaaacaatca cattaacatg ggattttgag gatgctacgg gagacactca
      841 tgaaacctct ggcatatttt ctgaatcctg gttatacaag tttcgcaatc caaacgaaga
      901 cgtaaacaag gttatcgaca aagacggaaa caaagtatat caagtaggtg cccttaccca
      961 gctggtatac aaggacaagc ttgaagatcc atacactctt tcatacagac tgcaaatggg
     1021 tcgcctgtag tccaatttga acaacggtgt tttcataaga gcagcagaaa tgcctgaagt
     1081 tgatggcaag accgtccact tttacgatag tgacggaatc ccaccatcag gaagctggat
     1141 ctatacaagc ggtctaagca ttgttttcaa acaccttggt tcgggagaca acccaaatcc
     1201 ttcattaaga ctaaacttta aatcttacga tgacaataca gaacagtatc ctatgaaagt
     1261 aaacaatcaa tacgttgaca tatcacttcc agaaggcacc aaccttgcca acttccatga
     1321 cattaagata acagatgacg gtacaaccat agaaattttc tttgacaatc aaaagcttgc
     1381 aactataaca atgtcggatg taggctcata tccggatgac attcctcaca aggatgtgga
     1441 attctacaag aaagcagtta taaaggatgc caacggaaac gaacttgcca attacgacaa
     1501 tgttcgtctg gccaaatcca gtgtaatctg ctttgcttcc aggaatgcac agccacaaaa
     1561 cttctcatac aaccttgaca atgttgttct gacaatcgaa aaagaagatg caccacagaa
     1621 tcctgagaca agcgatgcaa ttagcgtaat ggcattcgta attttaatgg ctttcatatc
     1681 tattctgatt aagagaagat ttgcttataa ctaaaaaacg agacgtttcg cctcattttt
     1741 ttcaactaat ttgagttatt gttgttatca tggaatatct ggtcaagaac aattacaaga
     1801 gccagcatta gcacttggtc ttcaccatcg tca
//
LOCUS       DAONVY010000476         1831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_42498_length_1831_cov_3.064752, whole genome shotgun sequence.
ACCESSION   DAONVY010000476 DAONVY010000000
VERSION     DAONVY010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42498_length_1831_cov_3.064752"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1831)
                     /locus_tag="PLI11_10410"
     CDS             complement(<1..>1831)
                     /locus_tag="PLI11_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPZ53321.1"
                     /translation="NDPLQLTCIKGINEEKAYKISEACIIHKDMSNFVGFLTKHGINA
                     KYAVKLYKKYGNMAIDLIKSNPYRLLEDIPRIRFEKVDALALQLDYPENSYGRVCAYI
                     IYILKSSYIRGHVYLPQMDIVYNVRTINVSEEDVLHCVGLLEEEGSIVVIDGEDDRHI
                     YLRYMYECQTFVANKLKALSSRIYYLNEDEVEKGIHSFEMQTGYTLALLQKEAIRTAA
                     SNGVSIITGGPGTGKTTIIKGILNLMTSLGQVVFLCAPTGRAAKRMSEMCSHEAKTIH
                     RLLEVSSDIENDDDYLVLFKKNESEPLECDVLIVDESSMIDIILMYHLLKAVKDGCRV
                     VFVGDKDQLPSVGPGKVLKDMLDSGLFATVTLNEIYRQESESLITINAHRINNGQYPK
                     LNVSDKDFFLIKRDTQERCLETLVDVCVNRLPQTYNIDPRRDIQVLIPTKRGMCGVLN
                     VNTVLQSYLNPQSKHKKERQVGEIVFREGDRVMQIRNNYNIEWERQSTLEYGKGVFNG
                     EMGILTSIDTKSKVAFVEFDDERLVCYEFEQMNDLELCYAITVHKSQGSEFDYIVLPL
                     FYTSPVLMTRNLLYTAITRAKKMVVLIGNEDCLVHMINNNTEEL"
BASE COUNT          496 a          446 c          306 g          583 t
ORIGIN      
        1 taagttcttc ggtattgttg ttaatcatgt gtacaagaca atcttcattg ccaataagga
       61 caaccatctt ttttgccctg gttatagcag tgtagagaag atttctggtc atcaaaacag
      121 gagaggtgta gaacaaaggc agtacgatgt aatcaaactc gctgccctgg ctcttatgga
      181 cagttatggc gtagcacagc tccaggtcat tcatctgctc aaactcatag catactaatc
      241 gttcatcatc aaactcaaca aaagcaactt tgctctttgt atcaatgctt gttaaaatac
      301 ccatttcgcc attgaacaca ccttttccat actccagagt gctctgtcgt tcccattcaa
      361 tgttgtagtt gttccttatc tgcatcaccc tgtccccctc cctgaagact atttctccaa
      421 cctgtctctc ctttttgtgt ttgctctggg ggtttagata actctgcaga accgtattta
      481 catttaacac accacacatt cctcttttgg taggtatcaa aacctgtata tcccgccttg
      541 gatcaatatt ataagtctgg ggcagtctgt tgacgcagac atcaaccagc gtttccagac
      601 atctttcctg agtatccctt tttatcaaaa agaaatcctt gtcgctcaca ttcaacttgg
      661 gatactgacc attgttaatc ctgtgggcgt ttattgtaat caggctttca ctctcctgcc
      721 tgtaaatctc attcagagtg actgtggcaa acagccccga atccagcatg tcctttaaaa
      781 ctttgcccgg accaacagaa ggaagctggt ccttatcgcc aacaaaaact accctgcagc
      841 catcctttac tgcttttaac agatgataca taaggattat gtctatcatg gatgactcgt
      901 ccacaatcaa cacatcacat tcaagaggtt cgctttcatt cttcttaaac agaaccaaat
      961 agtcatcgtc attttcaata tccgagctta cttcgaggag tcgatgaatc gtctttgctt
     1021 catgactgca catctccgac atcctcttgg cagcccttcc cgtaggagca caaagaaaca
     1081 ccacttgacc aagagatgtc ataaggttca atataccttt aataattgtt gttttaccgg
     1141 taccaggacc gcctgttatg attgaaactc cattgcttgc cgcagtcctt atggcttctt
     1201 tttgcaataa agcaagggta taccctgtct gcatttcaaa ggaatgtatg cctttttcaa
     1261 cctcatcctc attcaaataa tatattctgg acgaaagcgc cttgagcttg tttgccacaa
     1321 aggtctgaca ttcgtacatg taccgaagat aaatatgcct gtcatcttcc ccatctataa
     1381 cgactattga accttcctct tccagaaggc caacacaatg cagtacatcc tcttccgata
     1441 catttatggt tctcacgtta tatacaatat ccatttgagg caagtataca tgccctctta
     1501 tgtaactgct tttcagaata tatattatgt atgcgcagac ccttccatat gagttttcag
     1561 gataatccag ctgcagagcc aatgcgtcaa ctttctcaaa tcttattctg ggtatatcct
     1621 caagcagtct gtatggattg ctttttatca aatcaatggc catgtttcca tatttcttgt
     1681 acaatttaac cgcatacttt gcattaatac catgcttggt caaaaaacct acaaaattgg
     1741 acatgtcctt gtgaataatg caggcttcac tgattttgta agccttttcc tcatttatac
     1801 ctttgatgca agtcaattga agtggatcgt t
//
LOCUS       DAONVY010000477         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_42763_length_1821_cov_3.265572, whole genome shotgun sequence.
ACCESSION   DAONVY010000477 DAONVY010000000
VERSION     DAONVY010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42763_length_1821_cov_3.265572"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /gene="hisZ"
                     /locus_tag="PLI11_10415"
     CDS             <1..1215
                     /gene="hisZ"
                     /locus_tag="PLI11_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPZ53322.1"
                     /translation="CFNMRNLEADIRKHLRMCGYYEIITPTLEYYDVFTSGPNTMDSE
                     MMYKLTDKNGQILVLRPDLTIPAARVFTTKIVGDKAMPKKLFYIGNVFRFNEVGGGKT
                     NEFTQAGVEIMGQDGCLVDAEVIAKAIEISLLAGLENFQIDIGHSEFFIGIMEECGFS
                     DKETEETRLLVEKKDFAGLELMLKTKGVNDNVGELVLSIPQLFGDATILDKAALYAHN
                     EKSLNAIETLREILRILDDYKLSEYVSIDLGMVGSLKYYTGTIFKGYTYGVGFPYLGG
                     GRYDTLTGNFGKKIPASGFSLGINILMKALIRQGMCGLAPSCDAFVGFVRKYRKEAIE
                     ISQFFRENNISTHLDISEYDEGEAVEFARNEGFKLFLYVREDGKVRYINFNNGVDRII
                     DYQDIIEWGNAL"
     gene            1212..>1821
                     /gene="hisG"
                     /locus_tag="PLI11_10420"
     CDS             1212..>1821
                     /gene="hisG"
                     /locus_tag="PLI11_10420"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPZ53323.1"
                     /translation="MKYLNIALAKGRLAKQAADIFANTGINTSGLLEDSRKLIITDEE
                     NKVRFFLVKPSDVPTYVEYGAADIGICGRDTLLEEGRNLYEVIDLGIGKCRMCVAGPA
                     SLAGKLDNIPNKRVATKYPNISREYYQRVKRESVEIIELSGSVELAPLVGLSEVIVDI
                     VESGKTLQDNGLVVLETICDISARMIVNKVSMKIERVRISKIL"
BASE COUNT          578 a          282 c          463 g          498 t
ORIGIN      
        1 tgtttcaaca tgaggaacct ggaagccgac ataagaaaac atttgagaat gtgtggatat
       61 tatgagataa ttactcccac actggaatat tatgatgtct ttacatcagg acccaacacc
      121 atggatagtg aaatgatgta caaactgacc gacaaaaatg gtcagatcct tgtgttgcgt
      181 cctgatttga caattcctgc agcaagagtg tttacaacaa agattgtggg cgataaagca
      241 atgcccaaaa agctgtttta catagggaat gtattcagat ttaacgaagt gggtggaggc
      301 aagaccaatg agtttacaca ggcaggtgtt gaaatcatgg gtcaggatgg atgccttgtt
      361 gatgccgagg ttattgcaaa agctattgaa atttcgcttc ttgcaggtct tgagaatttt
      421 cagatagaca ttggacattc cgaattcttt attggcataa tggaggaatg cggcttcagc
      481 gacaaagaaa cagaagaaac cagactgctg gttgaaaaga aggattttgc aggtctggaa
      541 cttatgctca agacaaaagg agtaaacgac aatgtgggtg agcttgtgct tagtattccg
      601 caattatttg gggatgccac catccttgac aaggctgccc tgtatgcaca taacgaaaaa
      661 agtttgaacg ccatagaaac gttaagggag atattaagga ttcttgatga ttacaagctg
      721 agtgaatatg tatcaataga cctaggaatg gtgggaagct taaaatacta taccggaacc
      781 attttcaaag gctatacata tggcgtaggc tttccttatt taggaggagg aaggtacgac
      841 acccttacag gcaactttgg taagaaaata ccggcgtctg gtttcagtct tggcatcaac
      901 attttaatga aggctttgat tagacaggga atgtgcggat tagctccttc ttgtgatgcg
      961 tttgttggat ttgtcagaaa gtatagaaag gaagccattg agatatcgca gttttttaga
     1021 gaaaacaata tttctaccca tcttgatata tctgaatatg atgaagggga agcggttgaa
     1081 tttgccagaa acgaagggtt taaattgttt ctttatgtta gagaagacgg caaggtcaga
     1141 tacattaatt tcaacaatgg tgttgatcgc ataattgatt atcaggatat catcgaatgg
     1201 ggtaatgcgt tatgaagtac ttgaatattg cactggcaaa agggaggctt gcaaaacagg
     1261 cagcggacat atttgcaaat acaggcataa atacatcggg attgcttgaa gatagcagaa
     1321 agttgattat taccgatgag gaaaacaagg tgcgtttttt tcttgttaag ccgtcggatg
     1381 ttcccactta cgttgaatac ggtgctgcgg atattggcat atgtggaaga gacacactgc
     1441 tagaagaagg caggaattta tatgaggtta ttgacctggg cataggtaaa tgcaggatgt
     1501 gtgtagcagg tccggccagt cttgcgggaa agttggataa cataccaaac aaaagagttg
     1561 caaccaagta tccaaatatc tcaagggaat actaccagcg tgtgaaacgt gaaagtgtcg
     1621 agattattga actgtccggt tctgtggaac tggctcccct ggtggggttg tccgaggtta
     1681 ttgtggacat agttgagagc ggaaagacac ttcaagataa cggactggtg gttttggaaa
     1741 ctatatgtga tatcagtgct agaatgattg taaacaaggt cagtatgaaa attgaaagag
     1801 taaggatatc gaaaatattg g
//
LOCUS       DAONVY010000478         1812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43036_length_1812_cov_3.663631, whole genome shotgun sequence.
ACCESSION   DAONVY010000478 DAONVY010000000
VERSION     DAONVY010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43036_length_1812_cov_3.663631"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /gene="cysS"
                     /locus_tag="PLI11_10425"
     CDS             complement(<1..290)
                     /gene="cysS"
                     /locus_tag="PLI11_10425"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618380.1"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPZ53324.1"
                     /translation="MKIYNTLSRQKEEFKPLEGNEVRIYSCGPTVYNYAHIGNLRTYI
                     FMDTLRRVLRLNGYKLKHVMNITDVGHLVSDADEGEDKMAKAAKAQQKTPEE"
     gene            complement(265..1005)
                     /gene="cysE"
                     /locus_tag="PLI11_10430"
     CDS             complement(265..1005)
                     /gene="cysE"
                     /locus_tag="PLI11_10430"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPZ53325.1"
                     /translation="MFVGLLKDAKSIYKRDPAARSTLEVILLYPGFKALFYHRFSSFF
                     YRHKMYFIARYFSERARRKTGIEIHPGAKIGEGLFIDHGNGVVIGETAEIGNNCTIYH
                     QVTLGGTGTQKHKKRHPTVGDNVLIGAGAKILGPVKIGNNSLIGAGSVVLNDIPDNCT
                     VTGMKARIVKMDGQRVAPSETLEHTKVPDPINQELMKVMEAIKEREKNLQALIEDLNH
                     KINELECVKKKYEQKMEIDKNEDIQHTE"
     gene            complement(1116..1314)
                     /gene="ssrS"
                     /locus_tag="PLI11_10435"
     ncRNA           complement(1116..1314)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLI11_10435"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1372..>1812)
                     /locus_tag="PLI11_10440"
     CDS             complement(1372..>1812)
                     /locus_tag="PLI11_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HPZ53326.1"
                     /translation="DRIQDDVYAFPKPQNLANVCENELSDCIKAGYRCNYIVEASRLF
                     IEQPFSKEKFQETDIEKARRFMREYKGVGPKVADCILLFTGSFYNAFPVDVWVGKVMT
                     NLYKVSTQKEIDGFVQEYFGALAGFAQQYLFYAIREGFLTVQTT"
BASE COUNT          487 a          413 c          294 g          618 t
ORIGIN      
        1 atttcttccg gtgttttttg ctgtgctttt gcagcctttg ccattttatc ctcgccttca
       61 tccgcatcgg acacaagatg gcctacatcg gtaatattca ttacatgctt cagcttatac
      121 ccattcagcc tcaacactct tctaagggtg tccatgaaga tatatgttct taagttgccg
      181 atatgggcat aattgtacac ggtaggaccg caggaataaa ttcttacttc gttgccttcc
      241 aaaggtttga attcttcttt ttgtctactc agtgtgttgt atatcttcat ttttatctat
      301 ctccattttc tgttcatatt tcttttttac acattcaagc tcattaatct tgtgattaag
      361 gtcctcaatc aatgcctgaa ggtttttctc tctttccttg attgcttcca ttaccttcat
      421 caactcctga ttaatcggat caggaacttt tgtatgttca agagtttctg agggagctac
      481 cctctgtcca tccatcttca ctatcctcgc tttcattcct gttactgtgc aattgtcagg
      541 aatatcattt agaacaacgg agcctgcacc aatcagggag ttgttcccaa tctttacagg
      601 acccagtatt tttgctcctg cacctataag tacattgtcg ccaacagttg ggtgcctttt
      661 tttatgtttt tgcgtaccag ttccacccaa agttacctga tgatatatcg tacagttgtt
      721 ccctatttct gctgtctctc ctatgaccac gccattacca tggtcaataa acaatccttc
      781 gcctattttt gcgccaggat gtatttcaat accagtcttt cttcttgctc tttctgagaa
      841 gtatcttgca ataaaataca tcttgtgtct atagaagaac gatgaaaatc tatgataaaa
      901 aagagctttg aaacctggat atagaaggat tacttccaag gtgcttctag cagcaggatc
      961 ccttttgtat attgactttg catcttttaa taatcccaca aaaatcctcc tattattata
     1021 attgtgaata caattataca tcaaggtgca tgtaaagtaa agaataaacc ggatgaaaca
     1081 aatatgtttt catccggcaa gacataaaca gaagaaaata tcccaaaatg ccgccacctt
     1141 attttgacac ctagcctcgc taggtgggta tcctatggta tacttctgcc ttcctctgat
     1201 tcttgcgaat gacaccaagt gcctgaggcc tgacatccat atcccaaaat cggattcccg
     1261 tttatgtcat gtaggttcaa aactaaggtt cctcgtcgaa catttcagga taagttattt
     1321 ggtttttcat tagtattata tcatattttc cgcaaaacgc aacatgtaaa atcaggttgt
     1381 ctggactgtc aaaaatcctt cgcggattgc gtagaacaaa tactgctgcg caaatcctgc
     1441 aagagcgcca aagtattcct gcacaaaccc atcaatttcc ttttgcgtgg aaactttata
     1501 cagatttgtc ataacctttc ccacccatac atccacagga aaagcattgt aaaaagatcc
     1561 tgtgaatagc agtatgcagt ctgcaacttt tggtccaaca cctttatatt ctctcataaa
     1621 ccttcttgcc ttttcaatgt cagtttcttg aaatttttct ttggaaaaag gttgttcgat
     1681 aaacaagcgt gatgcttcca ctatataatt gcacctgtat ccggctttaa tacaatcaga
     1741 caattcattc tcacagacat ttgcaagatt ttgcggcttg ggaaatgcat acacatcatc
     1801 ttgtatcctg tc
//
LOCUS       DAONVY010000479         1812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43039_length_1812_cov_3.262379, whole genome shotgun sequence.
ACCESSION   DAONVY010000479 DAONVY010000000
VERSION     DAONVY010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43039_length_1812_cov_3.262379"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLI11_10445"
     CDS             <1..301
                     /locus_tag="PLI11_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPZ53327.1"
                     /translation="LLLGAVAKNTQTATLLSQAVFMPSLMLSGIMFPTSLLPTPLQMI
                     GNLFPATYINHAFRGLAYRFDTEIAPHTSMAIIMSVTILTLALSIRYLGKITSVR"
     gene            370..1479
                     /locus_tag="PLI11_10450"
     CDS             370..1479
                     /locus_tag="PLI11_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012859859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPZ53328.1"
                     /translation="MRPVTLLIKPASSLCNLRCTYCFYHDVAKKRLSPSYGLMTEKTL
                     EVLVRKVFQYAKGFAYFGFQGGEPTLVGLTFFEKLVELQKKYNIWGVKVINAIQTNGT
                     HLNKNWAEFFHKNNFLVGISLDGPPNIHDRFRLNADGKGSFDKVMGAVKLMEKHEVEY
                     NILCVVNNVVAENAARVYRFFKSNNIKYIQFIPCLNPFGDEHVYYDYTLTSENYLNFL
                     KTTFDLWYEDWSRGTPISIRTFDNYVSMIAGYPPEACGMSGVCSCYFLVESDGSVYPC
                     DFYVLDEYRLGNVLENSLEEMMETDTANAFVEESRIIHDKCKNCKWYRICRSGCKRNR
                     DPNTHENLYCDAYYNFFEYAYDKMDQIANILKRQI"
BASE COUNT          586 a          367 c          344 g          515 t
ORIGIN      
        1 tcttttgtta ggtgcagtag ctaaaaacac acaaactgcc actttgcttt cacaagctgt
       61 attcatgcct tctttgatgc tcagcggaat catgtttcca acaagtctgc ttccaacacc
      121 attacagatg attggaaacc tctttcccgc aacttatata aatcatgcct tcagaggttt
      181 ggcctaccgt ttcgacactg aaattgcccc acacacctct atggctataa ttatgtcagt
      241 tacaatatta actctagcct tgtccattcg gtatttgggc aaaatcacaa gtgtaagata
      301 acatatattg ttgactaatg cgggaatata aaataacata gaggtattaa acaaagaagg
      361 gagcaaactg tgagaccagt aacactgcta atcaagcctg catcgagctt atgcaattta
      421 agatgcacat attgtttcta tcatgacgtt gcaaaaaaaa gactgtcacc ttcatacggc
      481 cttatgacag agaaaaccct tgaagtcctt gtacggaaag ttttccagta tgcaaaaggt
      541 tttgcatact ttggttttca aggtggcgag cctacattgg taggcttgac gtttttcgaa
      601 aaactggtgg agcttcaaaa gaagtacaat atctggggtg ttaaagttat taatgccata
      661 cagacaaacg gaacacattt gaacaagaac tgggcagagt ttttccataa aaacaatttc
      721 cttgttggta tctccttgga tggtccgccc aacatacacg acagattcag attaaatgct
      781 gatggaaaag gttcttttga caaggtaatg ggtgctgtca aactgatgga aaaacacgag
      841 gttgaataca atattctgtg tgtagttaac aatgtggtgg cagaaaatgc agccagggtt
      901 tacaggtttt ttaaatcgaa caatataaaa tacatacaat ttattccctg tttgaatccc
      961 ttcggagacg aacatgtata ttacgactac actttaacca gtgaaaacta tttgaacttc
     1021 ctcaaaacca catttgatct ttggtacgaa gactggtcaa gaggcacacc aattagtata
     1081 agaacctttg acaattacgt gagcatgatt gctggttacc ctccagaagc ctgcggaatg
     1141 agtggtgtct gctcatgcta cttccttgtt gaatccgatg gaagcgtcta tccctgcgat
     1201 ttctatgttc ttgatgaata caggttaggg aatgtacttg aaaactcttt ggaagagatg
     1261 atggagacgg atactgcgaa tgcatttgtt gaagaatcca ggataattca tgacaaatgc
     1321 aaaaattgca aatggtacag gatctgcaga agcggatgca aaagaaaccg agacccaaac
     1381 acacacgaaa acctgtactg cgatgcatac tacaactttt ttgagtatgc atatgacaaa
     1441 atggaccaga tagcaaatat attgaagagg cagatatgaa aaatctggcg ttttttcgcc
     1501 agcctcttta acaactgagc aaaccaaacc ccttcccttg ccaattcgtc tatattcggc
     1561 gtcctaaccg gatatttccc attatatcat aacagtcata tctcaactaa tcagccgtta
     1621 ttaacagtac atagtatctc ctcttttgct ttttggctta tgatacaacc aaaaagcaaa
     1681 agaggagatg cgttccaaat actagattgc atgccgatat tactcattta agaaaactct
     1741 tgaacccaaa acggcctcaa gcatatatta cggtatattc acaagctcta tttcatcaaa
     1801 atagaagact cc
//
LOCUS       DAONVY010000480         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43320_length_1803_cov_3.557208, whole genome shotgun sequence.
ACCESSION   DAONVY010000480 DAONVY010000000
VERSION     DAONVY010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43320_length_1803_cov_3.557208"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="PLI11_10455"
     CDS             <1..1684
                     /locus_tag="PLI11_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53329.1"
                     /translation="PLFGYYNNGVDRWVLRKHAEMLADAGVDVVIFDQTNGTDTFMEA
                     IMTLLEVFAKARADGVKAPCITAMLPMFDYGNAAIQLREMYDKIYSKGLYKDLWFYWD
                     GKPLMLTWPGNLNINNPKDKEIFEFFTYRPINPSYREDDAQALGPDGEITIKWSPPQG
                     VREDYINWKWISIYPQAVAYRKDGTPEQMCVCIAQNWSKEQGITAMNAGDHVFGRSYS
                     DKNGYDLSKDGKLYGANFAEQFEYALKVDPDFIFITGWNEWVAGRFQEMWGTENAFPD
                     NFRDNYSRDIEPSKGDLKDHYYYQMVTYIRRFKGTDGFQRSSGKKKIDIYSDSDQWHD
                     VKPWFHAYEGNTFAREYDGYKGFSYFNDTGRNDIIGAKVAYDDNNIYFMVETRDDLTS
                     HTDKAWMRLFIDVNDSKDNWETFEYVINRINPSDKKAYLERSTGGYNWELVGEVDYAA
                     KGNRLQIAVPKKLLGIKGDKFEFNFKWADNTQEEGDVMDFYISGDTAPLGRFKYRFVS
                     ASQGKWLDFKNSPFPIIIIASLSLILLFSFVFFVWKIRLRKNINRGDTNERN"
     gene            1671..>1803
                     /locus_tag="PLI11_10460"
     CDS             1671..>1803
                     /locus_tag="PLI11_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPZ53330.1"
                     /translation="MREIKHHVDFCVVGGGMAGVCAALSAARHGVKVALMQDRPVLGG
                     "
BASE COUNT          587 a          329 c          431 g          456 t
ORIGIN      
        1 acccttgttt ggatattaca ataacggagt tgacagatgg gtactaagaa agcatgcaga
       61 aatgcttgca gatgcaggag ttgatgtggt catcttcgac cagacaaatg gcacagacac
      121 ttttatggaa gcaataatga cattgcttga agtgtttgcc aaagccaggg ctgatggtgt
      181 caaggcaccc tgcattacag ccatgctgcc tatgtttgat tatggcaatg ccgccatcca
      241 gctaagggag atgtatgaca aaatttactc aaaaggtcta tataaagacc tatggttcta
      301 ctgggatgga aagcctttga tgctgacatg gcctggcaat ctgaatatta acaacccaaa
      361 agacaaggag atatttgagt tctttacata ccgtccgata aacccatcat atagagagga
      421 tgacgcccag gctctgggac ctgatggtga aataaccatc aaatggagtc cgcctcaagg
      481 cgtcagggag gactatatca actggaaatg gataagcatt tatcctcaag cggtggctta
      541 cagaaaggat ggaacacctg aacaaatgtg tgtatgcatc gcacaaaact ggagcaagga
      601 acaaggcata accgcaatga atgcaggaga ccatgtattc ggccgttcct actctgataa
      661 aaatggatat gacttgagta aggacggaaa gctttatggt gcaaactttg ccgaacagtt
      721 tgaatatgct ttgaaggttg atccggactt catcttcata accggctgga atgaatgggt
      781 ggcaggcaga ttccaggaga tgtggggtac tgaaaatgca tttcctgata acttcagaga
      841 caactacagt agagatattg agccatccaa gggagatttg aaggatcact actactacca
      901 aatggtcacg tatattagac gtttcaaagg tacggacggt tttcaaagaa gcagcggcaa
      961 gaagaaaata gacatatact cggattctga tcaatggcac gatgtaaaac cttggtttca
     1021 cgcctacgaa ggaaatacct ttgcaagaga atatgacgga tacaaaggtt ttagttactt
     1081 taacgataca ggcagaaatg atattattgg cgcaaaggtt gcttacgatg acaacaacat
     1141 atacttcatg gttgaaacca gagatgattt aaccagccac acggataaag cctggatgcg
     1201 tttgtttatt gatgtaaatg actccaaaga taattgggaa acatttgaat atgtaataaa
     1261 cagaatcaat ccaagcgaca agaaagcata tttggaaagg tcaacgggag gatacaattg
     1321 ggaactggtt ggagaggttg actatgccgc aaaaggcaac agactgcaga ttgcggtgcc
     1381 taaaaaacta cttggcatta agggagacaa attcgaattc aattttaaat gggctgacaa
     1441 cacacaagaa gaaggagatg taatggactt ctatattagt ggagacactg ctcctcttgg
     1501 cagattcaaa taccgttttg tatcagcatc gcaaggaaag tggctcgatt tcaaaaacag
     1561 ccccttccca ataatcataa tcgcatcatt gtctctaata cttctatttt cttttgtatt
     1621 ttttgtctgg aagataagac taaggaaaaa tataaacagg ggtgatacca atgagagaaa
     1681 ttaagcatca cgttgatttc tgtgtagtag gcggtggtat ggctggtgtt tgtgctgcat
     1741 tgtctgcagc aagacacgga gtcaaagttg cacttatgca ggacaggcct gttctcggcg
     1801 gaa
//
LOCUS       DAONVY010000481         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43459_length_1799_cov_2.671445, whole genome shotgun sequence.
ACCESSION   DAONVY010000481 DAONVY010000000
VERSION     DAONVY010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43459_length_1799_cov_2.671445"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1700
                     /locus_tag="PLI11_10465"
     CDS             354..1700
                     /locus_tag="PLI11_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53331.1"
                     /translation="MFQKLGMEKRYFWIYKTFPYKGNSIKHWIKSDNTDYTLIRDGVI
                     TKDSKRWSRQPRPLEHLVRTIREVYVTNSSKVLVLGSKVDENKYVIGISFSGTVYNSE
                     WLNNFKVNIVNGFHQGFLEIAHRFDLLSDKIEFPNISKELGEETVLTLENILQECTSP
                     DSRFLLWVTGHSQGGAVAGCYIVDCLSNRNVLHDNIIGVTMATPTIAHARIGRSTTNY
                     PIYNIINEEDIVTRLGSQIRFGVDICYKPTEEFRRRFYTNYPNPLYHNDMVNARDFLE
                     EIKNTPDAIELTHAIQKLLSEYRPAKNGDNEKRLQHKTLKRLMTWLIFNLFSTQVVAS
                     EAYKEMTGRDIDESRINYWYKRIVDLYDGNKLAGLSEFVTQAHMLNFEQFHTTGPEHC
                     YRGIVLYAWDRTCVRCFKTDDAGYRYVEVASLWPKSEKGIVGFLGRLFSKRKKKKK"
     gene            complement(1701..>1799)
                     /locus_tag="PLI11_10470"
     CDS             complement(1701..>1799)
                     /locus_tag="PLI11_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HPZ53332.1"
                     /translation="DEVRKVLDTNKAERHIRGGLATKKKYEQESNI"
BASE COUNT          593 a          357 c          360 g          489 t
ORIGIN      
        1 atatgggtca acactaataa ctggattatt accacaatag gcaaacatat tgctcccaat
       61 aaatccttgc ccagtagtaa catatgcatc cgcattagtc ccgtctcatt ttcatagtta
      121 gtaacccttg taccttattg gattaatgta tcctatatgt gatgtgttgt tcgttacatc
      181 atccccattc tttatgctaa aagctttcta cagacatcgt aatggtatta ttccacctcg
      241 ttgaagtttt tgtcaagtat ggcagatata tcaatactgc atccgaccta atcactttat
      301 acaaaaatat ggtataatta taggaaatat aacaatatgg agcatataat ggattgtttc
      361 agaaacttgg aatggaaaaa agatattttt ggatatataa gacctttcct tacaaaggaa
      421 atagcattaa gcactggata aagagcgata acaccgacta tacccttata cgcgacggag
      481 ttattacaaa ggattcaaaa agatggtcaa ggcagccaag accgcttgaa cacctcgtac
      541 gcaccataag ggaggtgtat gtcaccaact ccagcaaggt gttggtgctg ggcagcaaag
      601 ttgatgaaaa caaatatgtt ataggcataa gtttttcagg aacagtatac aactcagagt
      661 ggctgaataa tttcaaggtc aatatagtta acgggtttca tcaaggtttt cttgagattg
      721 cccacagatt tgacttgctt tccgataaaa tcgaattccc caacatttca aaagaattgg
      781 gagaagaaac agtattaact ttggaaaaca ttcttcagga atgtacaagc cctgattcaa
      841 gatttttgtt gtgggtgaca ggacacagcc aaggcggcgc tgttgccggc tgctatattg
      901 tcgactgtct atcaaacaga aatgttttac atgataacat tataggagta accatggcca
      961 ctccgaccat tgcccatgcc aggataggaa gaagcacaac aaactaccca atatacaata
     1021 ttattaacga agaagatatt gtaaccagac tgggaagtca gataaggttt ggcgtagaca
     1081 tctgctacaa acccaccgag gaattcagaa gaaggttcta tacaaactat ccgaatcccc
     1141 tttaccataa cgacatggtc aatgcaagag attttcttga agaaataaaa aatacgcctg
     1201 atgcaataga gcttactcat gcgatacaaa aacttctgtc cgagtacaga cctgcaaaaa
     1261 acggagacaa tgaaaaaagg ctccagcata aaactttgaa acgcttgatg acctggctca
     1321 tattcaatct gttttcaaca caggtagttg caagtgaagc ctacaaggaa atgaccggcc
     1381 gcgatattga tgaaagcaga atcaactact ggtataaaag aatcgttgat ttgtacgatg
     1441 gcaacaaact ggccggcctt agtgaatttg tgacacaagc acacatgctg aacttcgaac
     1501 aatttcacac aactggtccg gaacactgct atagaggaat cgtattatac gcctgggatc
     1561 ggacttgtgt gcgctgcttt aaaacggatg atgcaggata caggtatgtg gaagttgctt
     1621 ctctgtggcc aaaatcagaa aaaggaattg tcggattcct gggtagactg ttcagcaaga
     1681 gaaagaagaa aaagaaatag ctatatattg ctctcctgtt cgtacttttt tttggtagcc
     1741 aggccacccc tgatatgtct ttcggccttg ttagtatcca acacttttct cacctcgtc
//
LOCUS       DAONVY010000482         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43464_length_1799_cov_2.384748, whole genome shotgun sequence.
ACCESSION   DAONVY010000482 DAONVY010000000
VERSION     DAONVY010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43464_length_1799_cov_2.384748"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLI11_10475"
     CDS             <1..317
                     /locus_tag="PLI11_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPZ53333.1"
                     /translation="CGKCAEACPYSAIVDIIRPCVKVCPVDAINMDENDIAVIDKDKC
                     INCGACVTGCPFGAISDVSMITNVINLLLDKSKKVYAVIAPSIQGQFGKNISLGMIKV
                     QF"
     gene            311..1054
                     /locus_tag="PLI11_10480"
     CDS             311..1054
                     /locus_tag="PLI11_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPZ53334.1"
                     /translation="MLNLGFDGVYEAAEGADSVAYEEAKELLEFKKEGKPLLSSCCPS
                     FFTLVENYFPELKDNVSTLVPPMAASAIYIKNMDPGAEIVFIGPCISKKADVLSNYID
                     IISGVLTFEELVAMLRAKRINMKNQDDISSNSSSYGKGFARSGGVSQAIRKAVSELEG
                     DESSSQLEIQICDGSKECINALKLLKHNRIRVDFVEGMCCTGGCIAGPSVLQDASNTR
                     KFFKPSDEDISLESAVKKLRINELDIHRT"
     gene            complement(1042..>1799)
                     /locus_tag="PLI11_10485"
     CDS             complement(1042..>1799)
                     /locus_tag="PLI11_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPZ53335.1"
                     /translation="VVSAVGNDDLGLESLRMVRDFGIDDRFIKVVEKPTGVCNVSVDE
                     QGNPTYDLVRDVAYDNITVDEEVFNSRYDAFYMGTLARRSKVSSETFRKLVEKCKYDE
                     VFCDINIRQDFYTKEIVEESLVHSTIIKISQEEAFVFNETGLLEYSNLEELSIKLAKD
                     FDIKLVLITLGEKGAFLYSAKENAFFKSIPAPVKVVSTVGAGDSFSAAFLYNYLNGKD
                     LQACLDKAVQLSSFVVTRLGAVPEFDPSNYQVR"
BASE COUNT          535 a          390 c          359 g          515 t
ORIGIN      
        1 actgcggcaa atgtgctgaa gcatgtccat attccgctat cgtggatatt ataagaccct
       61 gtgtgaaagt atgtcctgtg gacgccataa acatggatga gaatgatatt gctgtaattg
      121 acaaggacaa atgcataaac tgcggtgcat gtgtaacagg atgtcccttc ggtgcaattt
      181 ccgatgtctc gatgattaca aacgtaataa acctgcttct tgataaatca aagaaagtgt
      241 atgctgtgat tgctccctca atacaaggcc agtttggcaa aaacatatct ttgggaatga
      301 ttaaagtgca attctaaatc ttgggtttga tggtgtatat gaggcagcgg aaggagcaga
      361 ctccgtggca tacgaagagg caaaggaact tttagagttc aaaaaagaag ggaagcctct
      421 tctttcatct tgctgcccat ctttctttac tcttgtggaa aactattttc ccgaactaaa
      481 agacaatgtt tccactctag taccaccgat ggcagccagt gcgatatata taaaaaacat
      541 ggaccccggt gctgaaattg ttttcatagg tccatgcatt tcaaaaaagg cagatgtcct
      601 tagcaattat atagatatta tctcaggcgt gcttaccttt gaagaactcg ttgccatgct
      661 cagggcaaaa aggattaaca tgaagaatca ggatgacatt tcttctaatt catcttctta
      721 tggcaaaggg tttgcacgtt ctggtggtgt ttcccaggcc ataaggaaag ctgttagcga
      781 gctcgagggt gatgaatcat caagccagtt ggaaatccaa atatgtgacg gcagcaaaga
      841 gtgcattaat gccttgaaac tactaaagca caaccggatc agagtggatt ttgtagaggg
      901 catgtgctgc actggcggat gcattgccgg gccttccgtt ttgcaggatg cttccaatac
      961 gagaaaattc tttaaacctt cggatgaaga tatttccctg gaaagtgcag tcaaaaaact
     1021 acgaataaac gagcttgaca ttcaccgcac ctgatagttt gacggatcaa attctggtac
     1081 tgcgccaagc cttgttacaa caaaggaact taattgaaca gccttgtcaa ggcatgcctg
     1141 caagtccttc ccgttaaggt aattatatag aaacgctgca gaaaaactgt ctcctgctcc
     1201 gacagttgac accaccttca caggtgcagg tatggatttg aaaaacgcat tttcttttgc
     1261 ggaatacaaa aacgcgcctt tctctcccag cgtgattaat accaatttta tatcaaaatc
     1321 ttttgcaagc tttatggaaa gctcttcaag atttgaatac tccagcaaac ctgtttcgtt
     1381 aaagacaaag gcttcttcct gacttatctt tataatcgta ctgtgtacga gactttcttc
     1441 aacaatctct ttagtataga agtcctgccg gatatttata tcacaaaaca cttcatcata
     1501 tttgcatttt tctacaagtt tcctgaatgt ttctgacgaa accttgcttc ttctggccag
     1561 tgtacccata tagaaggcat cataacggga attgaaaact tcctcatcca cagtaatgtt
     1621 atcgtaagcc acatctctta ccaaatcata tgtagggttt ccttgctcat ccacacttac
     1681 attgcaaacg cctgtaggct tctcaacaac ctttatgaat ctgtcatcaa ttccaaaatc
     1741 ccgtaccatt cttaaagatt ccaggccaag gtcgtcattc cccacagctg aaaccaccg
//
LOCUS       DAONVY010000483         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43514_length_1797_cov_3.655568, whole genome shotgun sequence.
ACCESSION   DAONVY010000483 DAONVY010000000
VERSION     DAONVY010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43514_length_1797_cov_3.655568"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLI11_10490"
     CDS             complement(<1..655)
                     /locus_tag="PLI11_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53336.1"
                     /translation="MKRLVLFLLVIIFLQITVSAETVLLEDDLNAGTFSQTEFFTYFG
                     MYNANENNYEFDGENGYYHGKWTEGASAFYYDLLEDYTLYVDFATNGTTDRPKNAYRS
                     FIAIRVAETSKDFVFEPDNGQDQEAFAFLGVSGINFYCYENYVEVGVHTNKSGGTAGP
                     VNAPEGYGAVGEVDDASKRDFGVYSISAFFELPEGKTFDDFVSVKIVDEKDKVFFYAD
                     "
     gene            complement(811..1194)
                     /gene="acpS"
                     /locus_tag="PLI11_10495"
     CDS             complement(811..1194)
                     /gene="acpS"
                     /locus_tag="PLI11_10495"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPZ53337.1"
                     /translation="MKVYTGTDIVQVSRIKNAIMTSKESFLDKVYTKKEQDYCNSKRA
                     HMYESYAARFAVKEAVSKALGTGIGSDVGLLDVEVVNGERGKPNLILRGKAMELYNSL
                     NIFSSDISISHTSENAIAFVVMIGE"
     gene            complement(1214..>1797)
                     /locus_tag="PLI11_10500"
     CDS             complement(1214..>1797)
                     /locus_tag="PLI11_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53338.1"
                     /translation="KSRKAAEKQLIEIQTWAEMVRLDMREAEKRLRDVQDRIEVATLE
                     KDRILLAELLIQEEEYTEQYLEKKELHERAVQEALRVRDHFKHFEAEMKSKLRELQNI
                     RSQTQLARMRENIIALDNTYGAANGFGSSEMQESMDRLRRLVNERCARVMATEQLRRD
                     STEYRIRQIDHKSRWTKAQARADLLIAEKCGDI"
BASE COUNT          482 a          472 c          271 g          572 t
ORIGIN      
        1 catccgcata gaaaaacacc ttatcctttt catcgacaat cttcacactg acaaaatcat
       61 cgaaagtctt gccttcaggt agttcaaaaa acgcagatat tgaatacact ccaaagtccc
      121 tctttgaagc atcgtccacc tcaccaacag caccgtagcc ctctggtgca tttacaggac
      181 cagcagtgcc acctgatttg tttgtatgta cgccaacttc gacgtaattc tcatagcagt
      241 aaaagtttat gcctgaaacc ccaaggaaag caaaagcttc ctggtcctgg ccattatccg
      301 gctcaaacac aaaatctttt gaggtctcag ctacacgaat tgctatgaat gatctgtatg
      361 catttttggg acggtcggta gtaccgtttg ttgcaaaatc cacataaaga gtgtaatctt
      421 ccagcaaatc ataatagaat gcactggcac cttcagtcca tttgccatgg taataaccat
      481 tttccccatc aaactcatag ttgttctcat ttgcattgta catgccaaag tatgtaaaaa
      541 attctgtttg ggaaaacgtt cccgcattta gatcatcttc tagcaaaaca gtttctgcag
      601 atacagttat ctgtaaaaag ataatcacca gtagaaaaag caccaacctt ttcataaccg
      661 aaccctcctg aatttgtcca tatacttcca ctaattatca taccctgatt ctaattatct
      721 ttatgcagtc aaacaatgta caaaacatac ctttagttga aagtaataga catattgtaa
      781 taaaaagact gtgataaaaa tcacagtccg tcactcacct atcatcacta caaaagcaat
      841 cgcattttcg cttgtatggg aaatggatat gtcagaagaa aatatattca aagaattgta
      901 aagttccata gcttttccac ggagtataag gtttggtttt cccctttcac cattaacaac
      961 ctccacatcc agcaaaccca catcacttcc aataccggta cccaaagcct tggagacagc
     1021 ttccttaact gcaaatcttg cagcatagct ttcatacata tgtgcccttt ttgaattgca
     1081 gtaatcctgt tccttttttg tatacacttt atccaaaaaa gactctttcg aggtcataat
     1141 tgcattcttt attctactta cttgcactat atccgtgccc gtatagacct tcatttactt
     1201 aatcacatcc ctatcatata tctccgcatt tttcagcaat aagcaaatca gctcttgcct
     1261 gcgctttagt ccatcttgat ttatggtcaa tctgcctgat tctgtactct gtactgtctc
     1321 tcctaagctg ctctgttgcc attactcttg cacatctttc gtttaccagc cttctcaacc
     1381 tgtccatgct ctcctgcatt tcggagctgc caaatccatt tgccgctccg tatgtattgt
     1441 ctagagcaat gatattttct ctcatgcgag ccagttgcgt ctgcgacctt atgttctgca
     1501 gctctctcaa cttgcttttc atctctgcct caaagtgctt gaagtggtcc cgaactctta
     1561 acgcttcctg aactgccctt tcatggagtt ccttcttctc aagatactgc tccgtatact
     1621 cctcctcttg aatcagcagc tccgccagaa gtattctgtc tttttcaaga gtcgccacct
     1681 caattctgtc ctgcacatcc ctcagtcgtt tttccgcctc tctcatgtca agccggacca
     1741 tttccgccca ggtttgtatt tctatcaatt gtttctccgc tgccttccgg ctttttt
//
LOCUS       DAONVY010000484         1789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43754_length_1789_cov_4.574971, whole genome shotgun sequence.
ACCESSION   DAONVY010000484 DAONVY010000000
VERSION     DAONVY010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43754_length_1789_cov_4.574971"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLI11_10505"
     CDS             complement(<1..752)
                     /locus_tag="PLI11_10505"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPZ53339.1"
                     /translation="MLTDIQIAQSTKLKPITSVAESIGIKASDLELYGRYKAKISQEY
                     IKKSKDNKDGKLILVTSINPTPAGEGKTTITVGLGQAMNLIGKKTVIALREPSLGPTM
                     GIKGGATGGGYSQVLPMEDINLHFTGDLHAITAANNLLAAVIDNHIYQGNELDINVNR
                     VIHKRCIDMNDRALRNIVLGLGGTKDGIPRQDSFILTVATEVMAILCLSESLTDLKDR
                     LGRIIVAFTNSNKPITARDLNVQGAMALILKDA"
     gene            complement(1054..>1789)
                     /locus_tag="PLI11_10510"
     CDS             complement(1054..>1789)
                     /locus_tag="PLI11_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPZ53340.1"
                     /translation="TLPYAEEYMSVIFFGSVFQVVSIGLNHFIRADGSPKIAMLTMFF
                     GAGLNTVLDPLFIYVFRMGMAGAALATILSQMASASWVTLYFLGSKCKNRLRVRNMHL
                     KLKILYTIVILGMPNFLLQVANSFLNITLNKTIYTYGGNIAVSGMGVVNSIQTLLLMP
                     IIGLNQGVQPIVSYNFGAKKYDRVKSAAKSAIFAATTVVMIGFVITRLFPVQLVSMFN
                     RDEELLAFGKHAVKAWLCFSLWLVFR"
BASE COUNT          492 a          366 c          389 g          542 t
ORIGIN      
        1 gcatctttta aaatgagagc cattgcgcct tggacattca agtctcttgc ggtaattggc
       61 ttgttgctgt ttgtaaaggc aacaatgatt cttccaagtc tatctttcaa atctgtaagg
      121 ctttcagaaa gacacaggat tgccattact tcagtcgcca ctgtaaggat gaagctgtcc
      181 tgtctgggaa taccatcctt tgtgccacca aggccgagaa ctatgtttct taaagctctg
      241 tcgttcatgt caatacatct tttatgtatt acacgattga cattgatgtc aagttcatta
      301 ccttgataga tatgattgtc aatgactgct gcaagcaggt tgtttgcagc ggtaatagcg
      361 tgcagatcgc ctgtaaaatg caagtttatg tcttccatgg gtaacacttg ggagtatcct
      421 ccgcccgttg ctcctccctt tattcccatg gttgggccaa gagaaggctc tctgagggca
      481 atgacggttt tctttccaat cagattcata gcctggccca aacctacagt aattgttgtt
      541 tttccttctc ctgcaggtgt agggtttata gacgtaacca gtatcaactt gccatccttg
      601 ttgtccttgg atttttttat atattcctgg gaaattttcg ctttgtatct gccatacagc
      661 tcaaggtcag atgcctttat gcctatggat tctgctactg atgttattgg ttttaatttg
      721 gtgctttgag caatttgaat gtcagtcaac attgatgtac tcctttaatt atattttagc
      781 tacatattat atactgtatg ctttcttatt tcaaatgtat attctgtgct tgattctgca
      841 gttttgtaag gctcctgatt ccaaaaccga accagatgaa agtaagaaca aaggagaaga
      901 agtctgcgaa aggagcggcg taaagtattc ctttgaggcc gaataacctt gcaaaaacaa
      961 taattgcggg gagaagaagg ataacctgcc ttgttaatgt gaggaacatg gcagatttcg
     1021 ccctgcctat cgcttggaag aagcttgaac caattatctg aaaaccaacc acagggagaa
     1081 gcataaccac gcctttaccg catgttttcc aaaggcaagc agctcctcgt cgcggttgaa
     1141 catggagaca agctgaacag gaaacagcct tgtaattaca aaaccaatca tcacaactgt
     1201 tgtggctgca aaaatggcgc tcttggcagc actcttgacc ctgtcatatt tctttgctcc
     1261 gaagttgtag ctgacgatag gttgtactcc ctggttaaga ccgataattg gcatgaggag
     1321 aagtgtttgt atgctgttta caactcccat gccggataca gcaatgtttc caccataggt
     1381 ataaattgtt ttgtttaaag taatattaag aaagctgttt gcaacctgca ggagaaagtt
     1441 cggcatgccg agaataacaa ttgtatataa aattttcaat ttcaaatgca tgtttcttac
     1501 tctgagcctg tttttgcatt tactacccag gaagtataat gtgacccaag aagctgaagc
     1561 catttgggat agaatagtag caagtgcggc acctgccatg cccatcctga agacgtatat
     1621 aaacaggggg tcaagaacgg tgttgagacc tgctccgaag aacatggtca gcattgcaat
     1681 tttggggctt ccatctgcac gtatgaagtg gttgaggcca atacttacta cctggaagac
     1741 agagccaaag aaaatgacac tcatgtattc ttcagcatag ggaagagtg
//
LOCUS       DAONVY010000485         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43873_length_1786_cov_1.906990, whole genome shotgun sequence.
ACCESSION   DAONVY010000485 DAONVY010000000
VERSION     DAONVY010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43873_length_1786_cov_1.906990"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLI11_10515"
     CDS             complement(<1..1387)
                     /locus_tag="PLI11_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53341.1"
                     /translation="MVKIKRFFSILLSVSIFAAVIGCTKEEPKPKPRDTSNDTRLSKS
                     MVYANTIANKVQAYYTTHERDSYTITNTQMKLVHELGFSKEKNVSTLQTLKGNPYIEN
                     SLDVFIENNEGRRYYASKSPTSGRMNTTRLGFYYYESHIRELGFSTDEGELVGDKADI
                     TGLDAKDWKVHQMESPVYNNGVIVKVTNTHDPYVFRSGLDIDYTKYNGIEVKMKVEGA
                     AQLAEVYYFTADKGSFNAEQRLSFQVKNDGKFHTYVLDLAGAGEYKANLKGIRFDIGS
                     AVGDVITIEYVKAIQTSTRVVPFKLDKTFHTYPDKLHQEFRVIATSEASNIQNIGFEA
                     AFEKKNVAKIQYRDKNGVGTDISKMDSESVEYIAFDIKKAGVIGFIIPPDGSTKSVVV
                     TEEGSKYVLRQYANLSKNTFKRMDEESFGNRLYTDTSHSFDGIDKAAYLERNPLNDIN
                     ILDSNANARFVQ"
     gene            complement(1483..>1786)
                     /locus_tag="PLI11_10520"
     CDS             complement(1483..>1786)
                     /locus_tag="PLI11_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPZ53342.1"
                     /translation="EHLDDVVGLEEYKAKRDALYDVLVDAGFECRKPEGAFYMFPKSP
                     IPDDGEFAKEALKHNIVLVGGTGFGYPGYLRLSYCVSMDTIVNSRKAFKELMKKYR"
BASE COUNT          454 a          388 c          332 g          612 t
ORIGIN      
        1 attgaacaaa tcttgcattg gcgttcgaat caagaatatt aatatcattt agagggtttc
       61 tttcaaggta tgctgcttta tcaatgccgt caaaagaatg ggacgtatcg gtatacagtc
      121 tgtttccaaa ggattcttca tccattctct tgaacgtgtt ttttgaaagg ttggcatatt
      181 gtctcagtac atactttgag ccttcttccg tcacaacaac ggatttggta cttccatccg
      241 gtggaattat aaatccaata actcctgcct tctttatgtc aaaggcaatg tattccacag
      301 actcactgtc cattttggaa atgtcggttc cgactccgtt tttgtcccta tactggattt
      361 ttgccacatt ctttttctca aacgcggctt caaatcctat attctgaata ttggaagctt
      421 cactggtggc aattaccctg aattcctgat ggagtttatc cggatatgta tggaaagttt
      481 tatccagttt aaagggcaca accctggtgg atgtctgaat cgcctttaca tattctattg
      541 taattacatc acccactgcg ctgccgatgt caaaacggat tccttttaaa tttgccttgt
      601 attctcccgc tcctgccaaa tccaaaacgt atgtatggaa cttgccatca ttttttacct
      661 gaaaggaaag acgctgttct gcattgaaag aacccttgtc agcagtgaag taatatactt
      721 cagccaactg agccgcacct tccaccttca tcttaacctc tataccgttg tacttggtat
      781 agtctatatc cagaccggat ctgaatacat acggatcatg ggtgtttgta acctttacaa
      841 taacaccatt gttgtagaca ggactctcca tttggtgtac tttccagtct tttgcatcaa
      901 gtcctgtaat gtccgccttg tcgcctacca gttcaccctc gtccgttgaa aaacccagtt
      961 ccctgatatg gctttcgtag taatagaagc cgagtcttgt ggtgttcatt cttcctgatg
     1021 tgggtgattt tgacgcatag tatcttcgtc cttcattgtt ttcaataaat acatccaacg
     1081 aattttcgat gtagggatta ccttttaatg tttgaagggt tgatacgttc ttttcttttg
     1141 aaaagccaag ctcatgtacc agtttcatct gagtgttggt gatggtataa ctgtcccttt
     1201 catgggttgt gtagtatgcc tgaactttgt tggcaatcgt gtttgcatag accattgatt
     1261 ttgatagtct tgtgtcgttg ctggtatctc ttggcttggg ttttggttcc tcttttgtgc
     1321 agcctattac tgctgcaaat attgatacac ttagtagaat gctgaagaaa cgcttgattt
     1381 ttaccataaa aaaccccctg ttccatactc gttaatcttt aaaagtattg taacatatta
     1441 aggaatatgg aacaaggagt gcataattta ttaacaaatt aattatctgt attttttcat
     1501 aagctccttg aaggcttttc ttgaattgac tattgtatcc atgcttacgc aatatgaaag
     1561 ccttaggtag cctgggtacc caaaaccagt acctcctacc agcacaatat tatgcttaag
     1621 agcttctttt gcaaattcac catcatcggg aattggggat ttggggaaca tatagaaggc
     1681 gccttcaggt ttcctgcact caaaacccgc atctacaagt acatcgtaca gtgcatccct
     1741 ctttgccttg tattcttcaa gaccaaccac atcatccagg tgctct
//
LOCUS       DAONVY010000486         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_43906_length_1785_cov_2.172832, whole genome shotgun sequence.
ACCESSION   DAONVY010000486 DAONVY010000000
VERSION     DAONVY010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43906_length_1785_cov_2.172832"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /gene="priA"
                     /locus_tag="PLI11_10525"
     CDS             <1..1148
                     /gene="priA"
                     /locus_tag="PLI11_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPZ53343.1"
                     /translation="ATPNISTYYRATKGDVHLFTLKQRAKDVSLLPPIEIVDMRREMG
                     LGDNMFSQRLLQEMKKNLEANEQTVLFINRRGFSRNLQCQSCGKTMKCRRCNIPMTLH
                     LKQERIVCHYCGNTAPAPKTCPHCKSVSFIRKGIGTEKVEEALKKQFPGCTVVRMDTD
                     TTSVKDGHAKMIQKFRDERADFLVGTQMIAKGHDFPLVTLVGVINADSLINLQDYRAQ
                     EKAYQLLTQVSGRAGRDELPGRVIIQAYNIDDYAIECAANGVYEDFYRNEILVRQKLW
                     YPPFCEIGAVRFSGVNDKDVYDMAALHRKVLVGLVKDKGTEVLGPTRSGIPKINNKYR
                     WRLIVKCSDRDLLVDLFREYLEEIRKDNKYVKNDISMMYDINPGFMI"
     gene            1227..1715
                     /gene="def"
                     /locus_tag="PLI11_10530"
     CDS             1227..1715
                     /gene="def"
                     /locus_tag="PLI11_10530"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPZ53344.1"
                     /translation="MAVRQVRYEGDPVLRVKCKKVNEISPRIKRLIDDMFDTMYDLNG
                     VGLAAPQIGIVRRIVVIDDYNGAKYALINPYIVSEIGEQESREGCLSIPGYYGYVKRP
                     AKIHVKALNENGEEIEIKATGMLANILSHELDHLDGILYKDKAYEFARLEDDEELSDE
                     DE"
BASE COUNT          597 a          294 c          455 g          439 t
ORIGIN      
        1 cagccacacc caatatcagt acatattata gggcgacaaa gggagatgtg catctgttca
       61 ctcttaaaca aagagcgaag gatgtgtctt tgctccctcc aattgagata gttgacatgc
      121 gaagggaaat ggggcttggc gacaacatgt tctcacaaag actcctgcag gagatgaaaa
      181 agaaccttga agcaaacgag cagacagttc ttttcataaa cagaaggggt ttttcgagaa
      241 atttgcagtg ccaaagctgc ggaaagacca tgaaatgcag acgatgcaat ataccaatga
      301 cactgcattt gaagcaggag aggattgtct gccactactg tggcaatacc gcgccagctc
      361 caaagacatg cccccactgc aagagtgttt cttttataag aaaggggata ggtacggaaa
      421 aagttgaaga ggcgcttaaa aaacagtttc ccgggtgcac tgttgtaagg atggatacgg
      481 acacaacctc cgtaaaggac gggcatgcaa agatgataca aaagtttagg gatgaaaggg
      541 ctgacttttt ggtcggtacc cagatgattg caaaaggtca tgactttcca ttggtaacac
      601 tagtaggagt tattaatgca gattccctta ttaatctgca ggattacagg gcgcaggaaa
      661 aagcgtacca gctattgaca caagtgtctg gtcgtgcagg cagggatgaa cttcctggca
      721 gagttataat tcaagcatat aatatagatg actatgcaat tgaatgtgct gccaatggag
      781 tgtatgagga tttttacaga aatgaaatac tggtaagaca aaaactgtgg tatcctccgt
      841 tttgcgaaat tggagctgtc aggttcagtg gagtgaacga caaggatgtt tatgatatgg
      901 cggcattgca taggaaggtt ctggtgggtc ttgtgaagga taaagggacg gaggtgttag
      961 gtcctaccag aagcggtatt cccaagataa acaacaaata cagatggcgc cttattgtaa
     1021 aatgttcaga ccgggacttg ttggttgatt tatttagaga gtacttggag gaaataagga
     1081 aggataacaa gtacgttaaa aacgacataa gtatgatgta tgacataaat cccggcttta
     1141 tgatatagaa ttgaaaagag atgtcgtata tggtaatata tattgacaaa ataaaatata
     1201 aaatatcaat ataaaggaag agaattatgg cagttaggca ggtaagatat gaaggcgatc
     1261 ccgtactcag ggtcaagtgc aagaaggtaa acgagataag cccaagaata aaaagactta
     1321 tagacgacat gtttgacacg atgtatgact tgaatggagt cggtcttgcg gcgcctcaaa
     1381 tcgggattgt tcgcagaata gtggtaatag atgattacaa cggtgcaaaa tatgcactga
     1441 taaatccata tatagtcagc gaaatcggag agcaggaatc aagggaagga tgtctcagca
     1501 ttccaggata ttatggttat gtaaaaaggc cagccaagat acatgtcaag gcattgaacg
     1561 aaaatggaga agagattgag attaaggcca ccggaatgct tgccaacata ttgagtcatg
     1621 agcttgacca tttggatggc attctttaca aagacaaggc atatgaattt gccagacttg
     1681 aagatgatga agagctgtct gacgaggatg agtgattgat gaacaaaaga cttaagattg
     1741 tattcatggg cactcctgag tttgccctgc cttccttgaa agctt
//
LOCUS       DAONVY010000487         1770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_44410_length_1770_cov_3.135860, whole genome shotgun sequence.
ACCESSION   DAONVY010000487 DAONVY010000000
VERSION     DAONVY010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44410_length_1770_cov_3.135860"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLI11_10535"
     CDS             complement(<1..481)
                     /locus_tag="PLI11_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPZ53345.1"
                     /translation="MGSSSCSCSVADEKLLKLQKIIEENRNKKGALIHVLHEAQTIYG
                     YLPIEVQKVVAEGMGVPLAEVYGVVTFYAQFSLKPKGQYKISVCLGTACYVKGANLVL
                     EKLEEELGISVGDCTDDLRFSLEGCRCIGACGLAPVITINEDVYGRLSPNDVPNILAK
                     "
     gene            complement(646..>1770)
                     /locus_tag="PLI11_10540"
     CDS             complement(646..>1770)
                     /locus_tag="PLI11_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPZ53346.1"
                     /translation="EIVERRIIHDNKAEEMRILYVGLTRAREKLIVTGSIKEDCENWF
                     QNIRMKCDFETLSPVNFSILESKSFLEWIAMSLAYHHLNGRKDEKTACNWIIQSYTKD
                     EIEIDKLPDFVASFEKKEEVLEEDVDEKIHEKVSEILGYEYPCQTLSTVPSKVSVTEV
                     KRLMEEDFEDEFDLMNREVSFKELPDFMDEKTKDVSHALKGTLLHACLQQIDFNAVRN
                     IESEEDAKAYVDSLIDNMLKKRFMTEVQAKAIERKLVVLFILSPFAKRIMQADRVERE
                     VPFTIIKKWGELLGKRDEHADENVAVQGIIDCYFEEDGEIVVVDFKSDYIPQKNMMEV
                     VVNRYKIQIDLYKEALEKITGRKVKESYIYLLRYGEIISM"
BASE COUNT          510 a          438 c          255 g          567 t
ORIGIN      
        1 atttcgcaag aatatttggt acatcatttg gcgacaggcg gccgtacaca tcctcattta
       61 tggttatgac cggagcaaga ccgcaagcac ctatgcatct gcagccttcc agtgaaaacc
      121 ttaaatcgtc agtacaatcg cctacggata tgccaagctc ttcctcaagc ttttcgagta
      181 caaggtttgc acctttaaca tagcaggcag ttccaaggca cacggatatt ttgtattgtc
      241 cctttggctt caaagagaat tgcgcataaa aagtcacaac cccgtaaact tccgcaaggg
      301 gaacacccat gccctcggcg accactttct gaacttcaat tggcaaataa ccatatattg
      361 tctgagcttc gtgcaataca tggataaggg cgcctttttt gttcctgttc tcttcgataa
      421 tcttctgcaa ctttagcaac ttctcgtctg ctacgctgca tgaacaacta ctacttccca
      481 tacttttaaa ccctccaatt ttgaatatat aacacaattt attacacata caaagaatgc
      541 ataacttcac agttagcaaa catatgtatt atatcatcac agataattaa tatcaatggt
      601 ataatgttaa aaatctaaca aatcaacgat acattaacac atattttaca ttgaaataat
      661 ttcgccatat ctaagtagat aaatatacga ttccttaacc ttcctccccg ttatcttttc
      721 caacgcctcc ttgtataaat caatctgtat tttgtatcta tttacaacca cttccatcat
      781 gtttttctgt ggaatgtaat cgcttttaaa atccacaacc acaatttctc catcctcttc
      841 gaaatagcag tcaattatac cctgaacagc cacgttttca tcggcatgtt catccctttt
      901 tcccagaagt tcaccccact tcttgataat ggtaaaaggc acttcccttt ccaccctgtc
      961 cgcctgcatg attctctttg caaaaggaga aaggataaat agaacaacaa gttttctttc
     1021 aatagctttt gcctgcactt ccgtcatgaa tcgcttcttg agcatgttgt caatcagact
     1081 atcaacataa gctttagcgt cttcctcact ttcaatattt ctaacagcat taaaatcaat
     1141 ctgctgcaga caggcatgta gcaaggttcc cttaagcgca tgagacacat cctttgtttt
     1201 ctcatccatg aaatcgggca gttctttaaa ggaaacttcc ctgttcatca aatcaaactc
     1261 gtcttcaaaa tcttcttcca tgagtctctt gacttcagta actgaaacct ttgacggaac
     1321 agttgacaag gtctgacaag gatactcata gcccaatatt tctgaaacct tttcatgtat
     1381 tttctcatca acgtcttcct ctaaaacctc ttccttcttt tcaaaagaag ctacaaaatc
     1441 aggcagcttg tcaatttcaa tttcatcctt cgtatatgac tgtataatcc agttacatgc
     1501 agtcttttca tccttccttc cattcaaatg atggtatgca agactcatag caatccattc
     1561 aaggaagctt ttcgactcca ggatgctgaa gttaactgga ctcagagttt cgaaatcgca
     1621 tttcatccta atattttgaa accaattttc acaatcttcc tttatgctgc ctgtgacaat
     1681 taatttctct ctggccctgg taaggccgac gtaaaggatt ctcatctcct ctgccttgtt
     1741 atcatgtatt atgcgtcttt caacaatctc
//
LOCUS       DAONVY010000488         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_44605_length_1764_cov_3.879462, whole genome shotgun sequence.
ACCESSION   DAONVY010000488 DAONVY010000000
VERSION     DAONVY010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44605_length_1764_cov_3.879462"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLI11_10545"
     CDS             complement(<1..457)
                     /locus_tag="PLI11_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53347.1"
                     /translation="MKRYHVFIILIFSLVLAINIISNNKELDNWNHEGNVRFSNSTFV
                     VSTTDKNFVHKEVAGDKTITARVFDASNNAAAGLELRERDTDRLVSFISSDNEDVYLR
                     LVKTGNIVRQYKSKDGKAWELVDETSIPKLSDNLYLGMVVSSKDSKKKAQ"
     gene            605..>1764
                     /locus_tag="PLI11_10550"
     CDS             605..>1764
                     /locus_tag="PLI11_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53348.1"
                     /translation="MRKKLIFIALIVFLVSVIFVYSADDTVVSFFTDTEKNDFNLTLS
                     ERERFSVRFHASNSFDKLDIFFVSVKGSSKVKVSLYRWEGKLPEGQEALESREFSSLN
                     KSYASFDFNEKEKGEYLLEVEVVAGTAILRKSQYKRSQVDLYRNGYVEGGSIDVKLYY
                     TSKPEKDLEQLSSFHPPKHTPMPEPEYKMDEFAERIKPDTWTGVDGLGRTLPLYSNIK
                     KGKNDRIVGIFYWTWHGELSKASRARNITEIIKKYPEAINDFKHRAWEGYSGYHHWNE
                     PLFGYYSTNDEYVLRKHAEMLADAGIDFIAFDCTNGSYTWTESYMTLLKVFSEAKKDG
                     VRVPKITFMSNFAPIDATWNLVKQLYKELYRPGLYNDLWFYWEGKPLMWAYP"
BASE COUNT          534 a          311 c          382 g          537 t
ORIGIN      
        1 cctgtgcctt ttttttgcta tccttggacg aaacaaccat tccaagatac aagttgtcac
       61 taagcttggg gatgctcgtt tcatctacaa gctcccaggc ctttccgtct ttggatttgt
      121 actgtcttac aatattccct gttttgacaa gtctcaggta aacatcttcg ttatctgagc
      181 tgatgaagga taccagtctg tctgtatctc tttccctcag ttcaagacct gcagcagcgt
      241 tattacttgc atcaaacact cttgcggtta ttgttttgtc tcccgcaact tccttatgaa
      301 caaagttttt gtccgttgtg gaaacaacaa aggtggaatt tgaaaacctg acgtttcctt
      361 cgtgattcca gttgtctagt tctttgttgt tagaaataat gtttatggct aaaaccaaag
      421 aaaaaatcag tattatgaaa acatggtatc ttttcatttc aatctccttg ttggtttgat
      481 acgtatatta tacattacaa ccaaaagaaa aaccacacaa atgtgtggct atgcagacgt
      541 aattaaactt tgacacagga tttggctctc tgtataatca tgtttaggag aaggggtgtg
      601 caatatgcga aagaaactga tatttattgc tttgattgtg tttcttgtgt ccgtgatttt
      661 tgtatatagc gcagatgata ctgtagtgag tttctttact gatactgaaa agaatgattt
      721 caatttaacc ttatcggaaa gggaaaggtt ttcagtaagg tttcatgcat ccaattcctt
      781 tgacaagctt gacatatttt ttgtatcagt aaaaggaagt agtaaagtca aggtttcctt
      841 gtacaggtgg gaaggaaaat tgcctgaagg acaggaggct cttgaatcaa gagagttttc
      901 aagtctaaac aagtcgtatg catcgtttga ttttaatgaa aaagaaaaag gcgaatattt
      961 attggaagtt gaagttgttg caggaactgc aatactgagg aaaagccagt acaaacgctc
     1021 gcaggtggat ttgtaccgaa acggatatgt tgaaggaggc agtattgatg taaagcttta
     1081 ctacacaagc aaaccggaaa aggaccttga gcagttgtcc tcttttcacc ctccaaaaca
     1141 tacgccaatg cccgagcctg aatacaagat ggatgaattt gctgaaagaa taaagccgga
     1201 tacatggact ggagtggatg gtcttggcag aacactgcct ttgtattcaa acattaaaaa
     1261 gggaaaaaat gacagaattg ttggcatttt ttactggact tggcatggtg agctttcaaa
     1321 ggcaagtaga gcaagaaata ttacagagat aatcaaaaag tatcctgaag caataaatga
     1381 ttttaaacac agggcgtggg aaggctattc cggatatcac cattggaacg agcctctttt
     1441 tggatactat tccaccaatg atgagtatgt tttgagaaag catgcagaaa tgctggcgga
     1501 tgcgggtatc gattttattg cttttgactg tacaaacggc tcctatacct ggacggaatc
     1561 ctacatgact ttgctaaagg tgttttctga agcgaagaag gacggagtca gggtgccaaa
     1621 gataacattt atgtcaaact ttgctccaat tgacgccaca tggaatcttg taaaacaact
     1681 gtataaagaa ctgtatagac ccggtcttta caatgacctt tggttttact gggaaggaaa
     1741 gccgcttatg tgggcatatc ctca
//
LOCUS       DAONVY010000489         1760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_44766_length_1760_cov_3.057478, whole genome shotgun sequence.
ACCESSION   DAONVY010000489 DAONVY010000000
VERSION     DAONVY010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44766_length_1760_cov_3.057478"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1413)
                     /locus_tag="PLI11_10555"
     CDS             complement(34..1413)
                     /locus_tag="PLI11_10555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPZ53349.1"
                     /translation="MKSMEKIVALCKNRGFIYPGSDIYGGLANSWDYGPLGVELKNNV
                     KKAWWKKFVQESKYNVGVDCAILMNPRVWEASGHVAGFNDPLIDCKNCKARGRADKII
                     EEWANKQGEEINVDGWGDEQLAQYIKDNDVRCPQCGSANLTNIRKFNMMFKTYQGVTE
                     DSQAEIYLRPETAQGIFVNFKNVQRTTRRKIPFGVAQIGKAFRNEITPGNFTFRTREF
                     EQMELEFFCKPGTDLEWFEYWRTFCHKWLLDLGIADENLRLRDHSPEELAFYSKATTD
                     FEYKFPFAWGELWGIADRTDYDLKQHMEYTKEDMSYFDPETNEKYIPYCVEPSLGADR
                     VTLAFLCEAYEEEALPDGDVRTVLRFHPYLAPVKVAVLPLSKKLSDEAYKVYETLITK
                     FYCEFDESGSIGKRYRRQDEIGTPYCVTFDFDSLEDNKVTIRDRDTMEQTRVAIDELI
                     SWFEGKFDF"
     gene            complement(1425..1664)
                     /gene="rpoZ"
                     /locus_tag="PLI11_10560"
     CDS             complement(1425..1664)
                     /gene="rpoZ"
                     /locus_tag="PLI11_10560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPZ53350.1"
                     /translation="MMIEPPIAELLEKSGDRYTLVMMVSKRARQVTAGSQSRLDRDKI
                     MKDSIKDENRKSLTTAIYEVLEGKVNYVRKKEGIK"
BASE COUNT          434 a          449 c          308 g          569 t
ORIGIN      
        1 ctatatcatc aataaataat tgatattggc atattaaaaa tcaaactttc cttcaaacca
       61 tgaaattagt tcatcaattg caactcttgt ctgctccatt gtgtccctat ccctgattgt
      121 cactttgtta tcttcaagtg aatcaaagtc aaaggtgaca cagtaaggag taccaatctc
      181 atcctgtctt ctatatctct tgccgatgct gcctgattca tcaaattcgc agtagaattt
      241 tgttatgaga gtctcataaa ccttgtatgc ctcatcggaa agcttctttg aaagaggcag
      301 aacggcaacc ttgacgggcg caaggtatgg atggaatcta agtacagtcc taacatcgcc
      361 atcaggcaag gcttcctctt catacgcctc gcacaggaat gccagggtta ctctgtccgc
      421 accaagggaa ggctcaacac aatatggaat gtatttttca ttggtttcag gatcaaagta
      481 tgacatgtct tcctttgtat actccatatg ctgcttgagg tcgtagtccg ttctatcagc
      541 tatgccccac aactctcccc atgcaaaggg gaatttgtat tcaaaatccg ttgtagcctt
      601 gctgtagaac gccagctctt caggactgtg gtccctgagc ctaagatttt catcagcaat
      661 acccaaatcc agaagccact tgtggcagaa ggttctccag tactcaaacc attccagatc
      721 ggttccaggt ttgcagaaga attccagctc catctgctca aactctctgg ttctgaacgt
      781 aaagtttcca ggagttattt catttctgaa tgcctttcct atctgggcaa cgccaaaagg
      841 tatcttcctt ctggtggttc tctgtacgtt cttgaaattg acgaatattc cctgggctgt
      901 ctccggcctc aagtaaatct ctgcctggga gtcttcagta acaccttgat acgttttaaa
      961 catcatgttg aactttctga tattcgtaag gtttgcagaa ccacattgag ggcatcttac
     1021 atcgttatcc ttgatgtatt gggcaagctg ttcatctccc cagccatcca catttatttc
     1081 ctcgccctgt ttattagccc actcttcaat aattttgtct gccctgcctc ttgccttgca
     1141 gtttttgcag tcaatgagcg ggtcgttaaa tccggctaca tggccagacg cctcccatac
     1201 tctggggttc atcagaatag cgcaatcaac accaacattg tacttgcttt cctggacaaa
     1261 cttcttccac caggctttct ttacgttgtt cttcagctcc acacccaggg ggccgtagtc
     1321 ccagctgttt gcaagaccac cgtaaatatc cgaacccggg taaataaagc ctctgttttt
     1381 acaaagagca acgatcttct ccattgactt catcaaattt cctcctactt aatgccttct
     1441 ttttttctaa catagttgac cttgccttca agcacttcat atatggcagt tgtcaaactc
     1501 tttctgtttt catccttgat actgtctttc atgattttgt ccctgtcaag tctgctttgt
     1561 gaaccagcag ttacttgtct ggctctttta gagaccatca ttaccagcgt atatctatct
     1621 cctgactttt ccaataattc tgcaattgga ggttcaatca tcataactac ttcacccttt
     1681 ccctaaacaa gatcatattt ctgcaatatc cccttatttc tgaaactcct ccgtctttct
     1741 gcatgtatga tttttctaag
//
LOCUS       DAONVY010000490         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_45180_length_1747_cov_3.126478, whole genome shotgun sequence.
ACCESSION   DAONVY010000490 DAONVY010000000
VERSION     DAONVY010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45180_length_1747_cov_3.126478"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLI11_10565"
     CDS             complement(<1..617)
                     /locus_tag="PLI11_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ53351.1"
                     /translation="MIEIKSLTKDYKNVRALDNVTLTFEENKIYGLLGRNGVGKSTLL
                     NIITDKVFPSQGEVLVDGEKVSNNDEVLGKMFLMNEKNYYPEAMKVYEIFNWTNEFYP
                     DFDLVSAFEPADKFKLNTKSKVKALSTGYASIFKAIVALCSNTKYILLDEPVFGLDAN
                     HRDLLYKVILDRYANNPKTIIISTHLIEEVSSVIEEIVIIHNGKVL"
     gene            complement(610..981)
                     /locus_tag="PLI11_10570"
     CDS             complement(610..981)
                     /locus_tag="PLI11_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPZ53352.1"
                     /translation="MFIEFSDEKPIFIQIAEWIEDAILAGRYEEEQQIPSITEFSVAC
                     KINPATALKGINLLVDDGILYKKRGLGMFVANGAHEKLRTKRKELFYENYVISLVKEA
                     KRINIDKNDIMKMIERGFEND"
     gene            complement(1092..>1747)
                     /locus_tag="PLI11_10575"
     CDS             complement(1092..>1747)
                     /locus_tag="PLI11_10575"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPZ53353.1"
                     /translation="YYAHIEAIDDALGKLMDANDENTIFVYTSDHGDMLGSQGLGKKQ
                     KPYDESIRIPFLMKLPGESIKSFDEIFNTPDIAPTLLGLCGIDVPDTMEGRDLSEVIR
                     GKKKAEEAPAFIMNIAPFGEYARCFGGREWRGLRTKRHTYVISKAGPWLLFDNKKDPY
                     QLNNLIDSKDYTQIRQELHEELQKVMKERNDEFLFGDKYAQMWGYNLDDNGTVIYNW"
BASE COUNT          462 a          384 c          291 g          610 t
ORIGIN      
        1 aggacttttc cattgtggat aataacgatc tcttcaataa cactggacac ttcttcaatt
       61 aaatgggtgg aaatgattat tgtttttggg ttgtttgcgt atctgtccag tatgactttg
      121 taaagtaaat ccctgtggtt tgcatcaagt ccaaaaaccg gctcgtcaag caatatatat
      181 tttgtattag agcaaagagc aactattgcc ttgaaaattg atgcgtatcc agtggagagg
      241 gctttaacct tgctttttgt attaagcttg aatttgtctg ccggttcaaa agcactgacc
      301 aagtcaaagt caggatagaa ctcattcgtc cagttgaata tctcatacac cttcatggct
      361 tccggatagt aattcttttc attcatcagg aacatctttc cgagaacttc gtcgttgttt
      421 gaaacttttt ccccatctac caatacttct ccctggcttg gaaacacttt atctgtaatt
      481 atgttcaaca gtgtggactt gccaactccg tttcttccca aaagtccata tattttgttc
      541 tcttcaaatg tcaatgtaac attgtcaaga gctcttacat ttttataatc tttggttaaa
      601 ctcttaatct caatcatttt caaaccctct ttctatcatt ttcattatat cgtttttatc
      661 aatattgatt ctctttgcct ctttaacaag gctgatgaca tagttttcgt aaaacagttc
      721 tttgcgcttt gttcttaatt tttcatgcgc tccatttgca acaaacatgc ctagccccct
      781 tttcttgtat aaaattccat catccaccaa caggtttata cctttcagtg ccgttgcagg
      841 atttatcttg catgccactg aaaattccgt gattgaaggt atctgttgtt cttcctcgta
      901 tctgcctgca agaattgcat cctcaatcca ctccgctatc tgtataaaga ttggcttttc
      961 atctgaaaat tcgataaaca taagttcctc ctttctggct cattagaata ctggttagtt
     1021 agttgagtga ttaactaaaa ttagtaaaaa taaaacccta tcgaatgtca atagggttta
     1081 aaaaattttt tttaccagtt atagatcaca gtgccgttat catcaagatt gtatccccac
     1141 atttgagcat atttatctcc aaatagaaac tcatcgtttc tctctttcat aaccttttga
     1201 agttcttcat gaagctcttg cctgatttgt gtgtaatcct tgctgtcaat cagattattc
     1261 agctggtatg gatctttttt gttatcaaac aaaagccagg gtcctgcttt tgaaatgaca
     1321 taggtatggc gttttgtcct aagccccctc cattcccttc ctccaaaaca tcttgcatac
     1381 tcaccaaaag gggcaatgtt cattataaac gcaggagctt cttcggcttt cttctttcct
     1441 ctaataacct ccgacaaatc cctgccttcc atggtgtcgg gtacatctat tccgcacaaa
     1501 cccagcaaag taggtgcaat atccggagtg ttgaaaatct catcaaagct cttaatactt
     1561 tcaccaggca gtttcataag gaaaggtatg cgtatgcttt catcgtaagg cttttgcttt
     1621 ttgccaagcc cctgtgagcc aagcatgtct ccgtggtcgg aggtatatac gaaaatggtg
     1681 ttctcatcat ttgcgtccat gagtttgcca agggcgtcat caatggcctc gatatgagca
     1741 taatacc
//
LOCUS       DAONVY010000491         1733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_45670_length_1733_cov_2.776520, whole genome shotgun sequence.
ACCESSION   DAONVY010000491 DAONVY010000000
VERSION     DAONVY010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45670_length_1733_cov_2.776520"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLI11_10580"
     CDS             complement(<1..475)
                     /locus_tag="PLI11_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53354.1"
                     /translation="MNRLIRIFCLIFCVVILAGCGTDVKKVPPRDTSGDTKLSASMVY
                     ANSIANKVQAYYNKPDRSSLTIINTQVELVHELMGGSNKYVTALKTNKGKTYLKNTMD
                     VFVRDTSGSTFYASDSISTARVNTTRLGYYYYETHIRDLGFGKQDVTIYEKLDISG"
     gene            complement(591..1148)
                     /locus_tag="PLI11_10585"
     CDS             complement(591..1148)
                     /locus_tag="PLI11_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPZ53355.1"
                     /translation="MNREKRIFDTQSIVLCALFAALSTIGAYIKIPISYVPITMQLTF
                     TTLAGLLLGSCKGAISVSIYIIAGLIGLPVFTNGGGFGYVLQPTFGYILGFVAGAFVA
                     GLIVGKRKEVSYSRYLLASFGSIAACYTLGTIYFHIIMNLYLGNNMGLWKILMLCVIP
                     FIPGDVIMSLLASWVAKKIKYHLPL"
     gene            complement(1150..>1733)
                     /locus_tag="PLI11_10590"
     CDS             complement(1150..>1733)
                     /locus_tag="PLI11_10590"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPZ53356.1"
                     /translation="GVYMSYILRPQDDANIYNVVTVAACIAVCRAIYKLTGIECGIKW
                     VNDIFLNGKKICGILTEGSFDLENGRINLVIIGIGINVSTKDFPEDLEAVAGSICNSK
                     DGFVPRNRLVAEVLNELQSLTAPESLKEKKFIQEYREMSIVVGRDVEILVGENRKKAH
                     VIDIDDNGGLVVRCEDGKVESLNYGEISLRWNS"
BASE COUNT          523 a          416 c          282 g          512 t
ORIGIN      
        1 acccgcttat gtcaagtttt tcataaatgg taacatcctg ttttccaaat cccagatccc
       61 taatatgggt ttcataatag tagtatccaa gccttgtcgt attgaccctg gcagtactta
      121 ttgaatcaga cgcataaaat gtgctgccgc ttgtatccct gacaaaaaca tccatcgtat
      181 tcttcaagta tgtttttccc ttgtttgttt taagagccgt aacatactta ttgcttcccc
      241 ccataagctc gtggacaagc tcaacctggg tattgataat agtaaggctg cttctgtcag
      301 gtttgttgta gtaagcctgc accttgtttg caatactgtt tgcatacacc atggatgcgg
      361 ataatttggt atctccactg gtatctcttg gaggcacttt ttttacatcc gtaccacaac
      421 ctgccaatat aacaacacaa aaaatcagac aaaaaatacg aataagccta ttcatgtata
      481 actctcctcg caagtaatat gttaacaata taatatcaca aaaaggcaac taaaaaaact
      541 gccacttttc agtgacagtc cttgttacat catttttgtg cggctgtgtt tcacaagggt
      601 aagtggtatt tgattttctt cgcaacccag gaagcaagaa gactcataat aacatcacca
      661 gggataaaag ggattacgca aagcattaga attttccata aacccatatt gtttccaagg
      721 tacagattca taattatatg aaaataaatg gtacctagag tatagcaggc tgcaatgctc
      781 ccaaacgaag ccaaaagata tcttgaatat gatacttcct ttcttttgcc cacaatcaat
      841 ccggcaacaa aggcacctgc cacaaaacca agaatatagc cgaaggtggg ctgaagtacg
      901 tagccaaatc ctcctccatt tgtaaacacg ggaagtccaa tcaaccctgc aataatgtat
      961 attgaaacag agatggctcc cttgcaagag cccagaagca aacccgcaag tgtggtaaag
     1021 gtcagctgca ttgtaattgg aacatatgaa atcggtatct ttatatatgc tccaatggta
     1081 gacagcgccg caaaaagagc gcataaaaca atagattgtg tatcaaatat tctcttttcc
     1141 ctattcatgt caactgttcc acctcaatga tatttcacca taatttaaag actcaacctt
     1201 gccatcttca catctgacta caagccctcc attgtcatct atgtcaatga cgtgtgcctt
     1261 ttttctgttt tctcccacca agatctccac atccctgcct acaacaatcg acatctccct
     1321 gtactcctgt ataaacttct tttcctttag actttcagga gctgtaagag attgcagctc
     1381 attcaatact tctgcaacaa gccggtttct aggtacaaac ccatccttcg agttgcatat
     1441 tgagcctgcc accgcctcca aatcctccgg aaaatctttt gttgaaacat tgattccaat
     1501 accaatgatg acaagattta ttcttccatt ctccaaatca aaggatcctt ctgttagaat
     1561 tccgcatatc ttttttccat tcaggaagat gtcatttacc cactttatac cgcactcaat
     1621 gcctgtcagt ttgtaaattg ccctgcatac agcaatgcag gcagccaccg tcaccacatt
     1681 gtaaatattt gcatcatcct gcggtcttag aatatagctc atataaacgc cgg
//
LOCUS       DAONVY010000492         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_45734_length_1731_cov_2.889618, whole genome shotgun sequence.
ACCESSION   DAONVY010000492 DAONVY010000000
VERSION     DAONVY010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45734_length_1731_cov_2.889618"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..754)
                     /locus_tag="PLI11_10595"
     CDS             complement(50..754)
                     /locus_tag="PLI11_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPZ53357.1"
                     /translation="MNVAICDDNINYGKLAENYIREIVGPRRALNIDIFLSGQKLLES
                     INDKTYDIVFLDIEMPGINGIEVAQRLREKDKSVIIAFLTNYSEFATKGYEVSAFRYI
                     LKEEPESMIKRQVQSIVNEYYRKQKCLVANIKGVRITIKISSIIYLEVMKRVIYVHTA
                     SGKTYQFYSTLNNMLSRLEGFGFVKTHQSYVVNMSFIESIHVNSVVLKTGQSIPLSRV
                     LKREVETAYVSYLASL"
     gene            928..1641
                     /gene="fabG"
                     /locus_tag="PLI11_10600"
     CDS             928..1641
                     /gene="fabG"
                     /locus_tag="PLI11_10600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPZ53358.1"
                     /translation="MSRVVLITGASRGIGRAMAIKFAESGWLVAANYNKSEDRALQLK
                     RMSDNIYICKADVSNRNEVNNMIENIRRELGPICVLINNAAVSSIKMFSDITEEEWDY
                     TFDVNIKGVYNCTQEVLPDMIRKKSGSIINISSMWGQVGASCEVHYSAAKAAIIGFTK
                     ALAKELGPSFVRVNCIAPGVVSTEMNMDVDLSALREETPLGRLGTASEVAECAYFLAS
                     DNSSFITGQVIGVNGGLVV"
BASE COUNT          506 a          319 c          328 g          578 t
ORIGIN      
        1 atactgacca aacatttcac ttcatacctt actaatttat ttcttcttgt tacaaacttg
       61 ccagataact tacatacgca gtttctactt ctcttttcag aactctgctc aagggaatgg
      121 attgtcctgt tttcaacacc acactgttaa catgaatgct ctcaatgaac gacatgttta
      181 caacatagct ttgatgggtt tttacaaatc cgaacccctc aagtcttgac agcatattgt
      241 ttagggttga gtagaattga taggttttgc cagacgcagt atgtacataa atgactcttt
      301 tcatgacttc gagataaatt attgagctta tttttatagt tatcctgaca cctttgatat
      361 ttgcgacgag acatttctgc tttctgtagt attcattgac tatgctctgc acctgccttt
      421 ttatcatgct ttccggctcc tccttcaaga tataccggaa ggcacttacc tcataacctt
      481 tcgtcgcaaa ttcactgtag tttgtcaaga atgcaattat gaccgatttg tctttttcac
      541 gaagcctttg ggccacttct attccattta taccaggcat ttcaatatca agaaaaacaa
      601 tatcataggt tttatcattg atagactcaa gcaacttttg tccagagaga aatatatcga
      661 tattaagagc gcgtcttggt cctacgattt cccgtatgta gttttcagct agtttaccat
      721 aattaatatt gtcatcacat attgctacat tcatcaccat ctccccttgt ccattgaatt
      781 gcctatgttg ttttttgtac tgtgcaatat tctatcatac tttataaaat tttaaaatac
      841 ggcagccata ttggaatttt attgtattta ttgtttttta aacagaaaag ctgtatagta
      901 tagaaaaaac caagaaaggg aatttaaatg tcaagagttg ttctgataac aggtgcatca
      961 agaggtatag gcagggctat ggccatcaag tttgccgaat cgggatggct tgtggctgcc
     1021 aattacaaca agagtgaaga tagggcactg caactaaaga gaatgtctga caacatatat
     1081 atttgcaagg cagatgtttc gaatagaaat gaagttaata atatgattga aaatatcaga
     1141 agagagcttg gaccaatttg tgtgttgatc aacaatgcgg cagtgtccag cattaaaatg
     1201 ttttcagaca taacggaaga ggaatgggat tatacttttg atgttaatat taaaggtgta
     1261 tataattgta cccaggaagt cctccctgac atgatcagga agaaatccgg gagcattata
     1321 aacatttcat ccatgtgggg tcaggttggc gcatcctgcg aggtccatta ctcggcagcg
     1381 aaagctgcaa ttataggctt tactaaagca ttggcaaagg agctggggcc ctcatttgtt
     1441 agagtaaact gtatagctcc tggagtcgtt tcaacagaga tgaacatgga tgttgattta
     1501 agcgctttga gagaagaaac accattagga agattaggta ctgcttcaga ggttgctgaa
     1561 tgcgcatatt ttcttgcctc tgacaattct tcctttatta caggacaagt tattggtgta
     1621 aacggtggac ttgtagtata atttgcctgt gcttttccaa attttttgtt attttaacac
     1681 ttgacgtttt ttatatttat ggtacctgac ttccgtcgtt caactcaaaa c
//
LOCUS       DAONVY010000493         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_46270_length_1716_cov_3.645996, whole genome shotgun sequence.
ACCESSION   DAONVY010000493 DAONVY010000000
VERSION     DAONVY010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46270_length_1716_cov_3.645996"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLI11_10605"
     CDS             complement(<1..1092)
                     /locus_tag="PLI11_10605"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPZ53359.1"
                     /translation="MKFKHSLKLIIFLVAILTLVLGVNVEARDTHLKRLDFNIVLHEN
                     GDMTVTETWDVSFSESQHNVGFRTIDLEGLAGELIDIRVSQGSKEFTFAEYGENVHDP
                     DYKGQVGTFSTHVYNNELWIEWYINEFDSEKTYTLSYRVTDAVTVYNDTAELYWQFLG
                     TNFSMNVNKVTGKITIPQGANEENFRVWAHGPLNGTISREGTDTCLFEVKNFNTGDML
                     EVRLAMPVEIFPNSSNTVAKDYLDTILEEEQKYADEANARRKKLMIIGIVDLGVGVLA
                     LGFGILASILLNARYKRHAVVDDVEYYRDLPDKTMTPAQASHLYFFYNQAKRNRGNVV
                     SATILELARQKFIYFDEVLEKDKPDLGIYI"
     gene            complement(1103..1654)
                     /locus_tag="PLI11_10610"
     CDS             complement(1103..1654)
                     /locus_tag="PLI11_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPZ53360.1"
                     /translation="MWPAIIISGILVILIIIVISVYNKLVKLRLRVENQWSQVDVRLK
                     ERFDLIPNLVETVKGYASHEKETLENVINARNRAVTATTPEEAMDSEKSFQGALSRLF
                     ALAESYPDLKANTNFMDLQRKLSDMESQIAKYRQFYNDSVMRYNQTKAVFPNNIIANM
                     FGFEDKKFFTVETAEKEVPKVSF"
BASE COUNT          487 a          392 c          269 g          568 t
ORIGIN      
        1 tatatagatt ccgagatcag gcttatcctt ttccaaaact tcatcaaaat atatgaattt
       61 ctgccttgcc agttcaagaa ttgtggcact taccacatta cctctgtttc tctttgcttg
      121 attatagaag aaatatagat gggacgcctg agctggtgtc attgttttgt ctggcaaatc
      181 cctgtaatac tccacatcgt caactactgc atgcctcttg tacctggcat tcaacagaat
      241 agatgccaat atgccaaaac caagagcaag cacaccaaca ccaaggtcta caatccctat
      301 tatcataagt tttttacgcc ttgcatttgc ttcgtcagca tacttttgct cttcttcaag
      361 gattgtatca aggtaatcct tggctactgt attggatgaa ttcgggaata tctcaacagg
      421 cattgcaaga cgcacttcaa gcatgtctcc tgtattgaag ttctttacct caaacaaaca
      481 agtgtccgtt ccttctctac ttatggtgcc attcaaaggg ccatgggccc acactctgaa
      541 gttttcctca tttgcacctt gcggaattgt aatctttccc gtcaccttgt ttacattcat
      601 tgaaaagttt gtgccaagga actgccagta aagttctgcc gtgtcattgt ataccgttac
      661 cgcatccgta acccgatagg ataaggtgta cgttttttca ctatcaaatt cgtttatata
      721 ccattcaatc cataattcat tattatacac atgcgtactg aaggtcccaa cttgtccctt
      781 gtagtcagga tcatgcacat tttcgccgta ttccgcaaat gtaaattctt ttgaaccctg
      841 agaaaccctg atatcaatta gttctcctgc aagtccttcc aagtctatgg ttctaaaacc
      901 aacattatgt tggctttcag aaaaagacac atcccaagtt tcagtgacag tcatgtcacc
      961 gttctcatgt agtacaatat tgaaatccag tctctttaga tgagtatccc tggcctcaac
     1021 attaactcca agcacaaggg tcaatattgc aaccaggaat atgatgagtt ttaagctatg
     1081 tttgaatttc atgacaatcc cttcaaaaag atacctttgg cacttccttt tcagctgttt
     1141 caaccgtaaa gaacttcttg tcttcaaatc caaacatatt ggctataata ttgtttggaa
     1201 aaacagcctt agtctgattg tatctcataa cactgtcatt gtaaaactgt ctgtactttg
     1261 caatctgaga ctccatatca ctgagttttc tctgcagatc cataaaattg gtattcgctt
     1321 taagatccgg ataactctca gccaatgcaa acaatctgct caaagcgcct tggaaacttt
     1381 tctcactgtc catggcttct tcaggagtag ttgcagtaac tgccctgttt cttgcattaa
     1441 ttacattttc caatgtctct ttctcatgag atgcatatcc tttgacggtt tcaacaagat
     1501 tgggaatcaa atcaaaacgt tccttcaatc gtacatccac ctgggaccat tggttttcca
     1561 ctctcaatct aagttttaca agtttattgt atacacttat aactatgatg atgagtataa
     1621 ctaatattcc tgatataata attgccggcc acataaaaac aacctcctaa tcaagattta
     1681 aacatttgtg tttcaatata tatttgtata catttt
//
LOCUS       DAONVY010000494         1701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_46811_length_1701_cov_3.013973, whole genome shotgun sequence.
ACCESSION   DAONVY010000494 DAONVY010000000
VERSION     DAONVY010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46811_length_1701_cov_3.013973"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLI11_10615"
     CDS             <1..311
                     /locus_tag="PLI11_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010531961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HPZ53361.1"
                     /translation="VHPIHIHLIQFQVLDRIPFDVDAHNRNGQIRFTGSPVPPEPQEA
                     GWKDTIATPPGFITRLIMKFAPYTGRYVYHCHILEHEDYDMMRALDVLPPDKCKRKCK
                     "
     gene            463..>1701
                     /locus_tag="PLI11_10620"
     CDS             463..>1701
                     /locus_tag="PLI11_10620"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPZ53362.1"
                     /translation="MDYFKALSYCESFAANKSSMSLENMKTLLGLLGNPHEKVKHIHI
                     AGTNGKGSVVSFIAEILTTSGLKVGKFVSPHLMRINERFSIDGQDIDDNEFTSCVAEI
                     KKAITGHKELKNSLTMFEIMTCVAFLHFNKNDCDIAVLETGLGGRFDATNVIQNPLLT
                     VITMIGFDHTKVLGDTIERIAFEKACIIKKSSMCVIAPQKYIDALSVLEKHAENQGVE
                     PILVDKSAIVTKERSASSQIFDYKNLKELKITLLGNHQTENAATAVEAVSALGKLGFD
                     INKEAIYKGLERAKWPCRLEVVNTEPITIIDGAHNVDGVMALSGFLRDTFRDKKITYI
                     FGVMKDKNYKAMIQEIKHNAKEVYLIEMQYYRAENIDILKSVLNEYGIASRTFATLED
                     AIDYSYRQLSKDDIVCIFGSL"
BASE COUNT          572 a          297 c          377 g          455 t
ORIGIN      
        1 cagtccaccc cattcacatc caccttattc aattccaggt gcttgaccgc ataccctttg
       61 atgtggatgc gcacaacaga aatggacaga taaggtttac aggaagcccc gtgcctcccg
      121 agcctcagga agcaggttgg aaggacacaa ttgctactcc ccctggattc ataacaagat
      181 tgataatgaa atttgctccg tataccggca gatatgtata ccattgtcac atactggagc
      241 atgaagacta tgacatgatg agagccttgg atgttttacc tcccgataaa tgtaaaagaa
      301 aatgcaaata aaaaatgcat tccataaaat gtaccgatac ctccaatcca gcccttgcgt
      361 tatgcagggg ctgacttctt gctgcaaaag gggtgttgtg ctatactcac atattgaacc
      421 taaatattaa acattaccag gtatgcttgg aggatgcatt taatggacta ctttaaggca
      481 ctttcctatt gtgagagttt tgcagcaaac aaaagcagca tgagtctgga aaacatgaaa
      541 acactgctcg gtttgcttgg caatcctcat gagaaagtaa aacatataca catagcagga
      601 acaaatggaa aaggttctgt tgtgagtttc atagctgaaa tacttacaac atccggattg
      661 aaggttggca aatttgtctc tccgcatctt atgagaatta atgagaggtt ttcaattgat
      721 ggtcaagata tcgatgataa tgagtttaca tcatgcgttg cagagattaa aaaagcaata
      781 accggacaca aagaactgaa aaacagcctt accatgtttg aaataatgac atgcgtagcc
      841 tttctgcact ttaacaaaaa tgattgtgac attgcagtgt tggaaaccgg tcttggtgga
      901 aggtttgatg ctacgaatgt aatacaaaac ccattgttga ctgtaatcac gatgatcggt
      961 tttgatcata caaaagtact cggagatacg atagaaagga tagcttttga gaaagcatgc
     1021 attataaaga aaagcagcat gtgtgttatt gcgccgcaaa aatatattga tgcattgagc
     1081 gtgcttgaga agcatgctga aaatcaggga gttgaaccta ttcttgttga caagagcgcg
     1141 attgtcacaa aagagaggag tgcaagctcc cagatatttg attacaagaa tttgaaagaa
     1201 ttaaagatta ctcttcttgg aaaccatcaa actgaaaatg cggcaactgc agtagaggca
     1261 gtatccgccc ttggcaaact gggttttgat attaataaag aagcaatata taaagggctt
     1321 gaaagggcaa aatggccttg caggcttgag gtggtgaata cagagccaat aacgataatt
     1381 gacggcgcac acaatgtgga cggagtaatg gcgttgtcgg gttttttaag agataccttc
     1441 agggataaga agataacata tatttttggc gtaatgaagg acaagaacta caaggccatg
     1501 atacaagaga taaaacacaa tgcaaaagag gtatatttga tagagatgca atactaccgg
     1561 gctgaaaata tagatatatt gaaatcagtt ttaaatgaat atggcatagc aagcaggact
     1621 tttgcaactc ttgaagatgc gattgattat tcgtacaggc aattgtcaaa ggatgatatt
     1681 gtttgtattt ttggttccct t
//
LOCUS       DAONVY010000495         1698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_46908_length_1698_cov_6.216677, whole genome shotgun sequence.
ACCESSION   DAONVY010000495 DAONVY010000000
VERSION     DAONVY010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46908_length_1698_cov_6.216677"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..816
                     /locus_tag="PLI11_10625"
     CDS             34..816
                     /locus_tag="PLI11_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPZ53363.1"
                     /translation="MNEDCLFTVGELAKKCGVTVRTLQYYDQCGLLSPSRYSEGGRRL
                     YSIDDVLRLQQILFFKGFGFTLEEIRNRLMTINSAQDFAKLLAKQKNVIEEQIRHLEK
                     IVSLMDETIERIGPSENLSINMVMAILKAAKQNDFYSLVIDSIDKKYMDSFLLNEAKT
                     DLKDWDSLLTRLKELHKKDADPFGQEAEKLAEDWWNMIMDLTGGDPNLIATVFSAGES
                     LPNWPEETDDIKTAIEKILSPALGSYLLKQGIVPPGMEDGFK"
     gene            813..>1698
                     /locus_tag="PLI11_10630"
     CDS             813..>1698
                     /locus_tag="PLI11_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ53364.1"
                     /translation="MTLAVQVNSLVKKYGSNTAVDNISFNIYKGEVFGLLGPNGAGKT
                     TTLEIIEGLRMPTLGEVYVEGLSPIRDTKELRKVLGVQLQISTLPEVMRVDEAMELIC
                     TFKGIKPRYDLLERFGLVKQKKQQYGKLSTGQKRRLQLALAICGNPKVVILDEPTAGV
                     DVQGRAQLHNAIRELRKNGVTVVIATHDMAEAESLCDRIAIIINGKLAALGSPDEVTA
                     KGSFQTRITIRTKKGNLTSQGNIKKATFASQKENNALWLCTDTASAIIEMLSAIDADD
                     EVLVLRVERPSLEERFLEL"
BASE COUNT          585 a          310 c          380 g          423 t
ORIGIN      
        1 taatgtttta tactatcagg gaggtgataa ataatgaatg aagactgctt gtttacagta
       61 ggagaacttg caaaaaaatg tggagttact gtgcgaacgc tgcaatacta cgaccaatgc
      121 ggattacttt cgcccagccg ctactcggaa ggtggaagac gcctttacag tattgatgat
      181 gttttgcgtt tgcagcaaat cctgttcttc aagggctttg gattcacgct tgaagaaata
      241 cgcaatagat taatgacaat aaactcagcc caggattttg ccaaattact tgcaaaacaa
      301 aaaaatgtca tcgaggaaca aataagacat ttagagaaga tagtatctct tatggatgaa
      361 accatagaga gaataggccc tagtgagaac ctctcaataa acatggttat ggcaatattg
      421 aaagctgcaa aacaaaatga cttttattct ctggtaattg acagtataga caagaaatac
      481 atggattcct ttttattaaa tgaagccaaa accgacttaa aagattggga ctctcttttg
      541 acaagattaa aagaattaca taagaaagat gcagaccctt ttggacagga agcagaaaaa
      601 ctggcagaag actggtggaa catgataatg gacttgacag gaggagatcc taatttgatt
      661 gctactgtat ttagtgctgg agagtccttg cccaactggc cggaagaaac tgatgacata
      721 aagactgcca ttgaaaagat cctatcccct gctcttggca gttacctgct aaaacaaggt
      781 atcgtcccac cgggcatgga ggatggtttc aaatgactct ggcagttcaa gtaaactcgt
      841 tggtaaagaa atacggttca aatacggctg tcgacaatat ctcttttaat atatataaag
      901 gcgaagtctt tggtctgttg ggaccaaatg gagccggaaa aaccacaact cttgaaatta
      961 ttgaaggtct gcgcatgcca acattgggag aagtttatgt agaaggccta agtccaataa
     1021 gagatacaaa agaactaaga aaagtcctgg gagttcaatt gcagatatcc acgctgcctg
     1081 aagtcatgcg tgttgacgag gcaatggaac ttatttgcac cttcaaaggc ataaagccca
     1141 ggtacgattt gcttgaaagg ttcggattgg taaaacaaaa aaagcagcaa tatggaaaac
     1201 tctctacagg acaaaagaga agacttcaac ttgccttggc tatctgtggc aatcccaaag
     1261 ttgtaatact ggatgaacca acagcaggtg tagatgttca aggaagggcg cagcttcata
     1321 atgccattag ggaacttaga aaaaatggcg ttacagtagt tatagcgact catgacatgg
     1381 cagaggccga aagcttgtgt gacagaatcg ccataatcat aaacggcaaa cttgcagctt
     1441 tgggaagccc cgatgaagta acagcaaaag gcagctttca aacaagaata accatccgca
     1501 ccaagaaagg aaatttaact tcacaaggta atattaaaaa agccactttt gcaagccaaa
     1561 aggaaaacaa tgccttatgg ctctgcacgg atacagcttc tgcaataatt gaaatgctgt
     1621 ctgccattga tgcagatgat gaggttcttg ttctgagagt ggaaagaccg tctcttgaag
     1681 aaagattctt ggaactta
//
LOCUS       DAONVY010000496         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_47107_length_1694_cov_2.358145, whole genome shotgun sequence.
ACCESSION   DAONVY010000496 DAONVY010000000
VERSION     DAONVY010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47107_length_1694_cov_2.358145"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1004)
                     /locus_tag="PLI11_10635"
     CDS             complement(75..1004)
                     /locus_tag="PLI11_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPZ53365.1"
                     /translation="MLKTVNLTKYYGHFLALDKLNIEVREGEIYGFVGPNGAGKTTAM
                     KILACLMNASAGEAYIDGISIMQNPAKAKKLIGYMPDFFGVYDNLKVDEYLEFYADAH
                     NVPKSERAPLIEDLLQLVNLSDKKDKYVDTLSRGMKQRLCLARSLIHDPKLLILDEPA
                     SGMDPQARAEMKFILKALKAQGKSILISSHILPELSEMCDRVCILSRGKSVVSGNMDE
                     IMEAMGRRAKITIGFLTDEDLQNGVAVMRTNDYVGQMVREGNKVEAVFNGTDEDMSKL
                     IKAFALSDVRIVSISKTVQSLEQIFLEVIGNEN"
     gene            complement(1018..>1694)
                     /locus_tag="PLI11_10640"
     CDS             complement(1018..>1694)
                     /locus_tag="PLI11_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53366.1"
                     /translation="YGNTQINSLISQLGREEGVKAALLIKLLSQKLGATEFSMSPQMH
                     IAGFVVDGPDVNVKINGRKPTKAYNNTLVHQSVSLHGYVDLQDEEKYLPVFLEDFSFV
                     KDVKGDSVVLDDSKHYALYEISVPKEYVHDKNLVLNINTTENVEIVINNFIFDTSTVE
                     HISRGISPFEFTISKYSYRDYSLRRSYVYEPVSEYVFFHIRIEPVDKSQPLTIESVTY
                     EFSDNK"
BASE COUNT          448 a          398 c          317 g          531 t
ORIGIN      
        1 caagaagagc aataggcgat ctccagcttc tcatcctgat tttcatttct gaagatatta
       61 tgggattatt caatttaatt ttcattgcca attacctcca ggaagatttg ttcaagggat
      121 tgaaccgtct tgcttatgga cactatcctt acatcagata gtgcaaatgc ttttatgagt
      181 tttgacatgt cttcgtccgt accgttaaaa actgcttcta ctttgtttcc ttccctaacc
      241 atttgtccca cataatcatt ggtgcgcatt actgcaactc cattctgcag atcctcatca
      301 gtaaggaaac ctatagtaat ttttgcccgt ctgcccatgg cttccattat ctcatccata
      361 ttacctgata ccacagactt tcctcgtgaa agaatgcaga ctctatcgca catttcggaa
      421 agctcgggca gtatgtggga acttatgagt atggacttgc cctgggcttt cagcgccttc
      481 aaaatgaact tcatttccgc tcttgcctga gggtccatgc ccgatgcagg ttcgtccaga
      541 attagaagct tgggatcgtg gataaggctt cttgcaagac agagtctctg cttcatacct
      601 cttgaaagag tatctacata tttgtccttc ttgtctgaca ggtttacaag ctgcagcaga
      661 tcttctatta aaggagctct ttcactcttt ggaacattat gggcgtcagc gtaaaactcc
      721 agatactcat ccactttcaa gttgtcatac accccgaaaa aatcgggcat gtagcctatg
      781 agtttttttg cttttgccgg attctgcata attgaaatgc catctatgta agcttctcct
      841 gcagaagcat tcataaggca tgccagaatc ttcatagcag tggtcttgcc cgcgccatta
      901 ggaccaacaa aaccatatat ctcgccttcc ctgacctcta tgttcaattt gtccagagca
      961 aggaaatgac cgtaatattt tgtcaaatta actgttttca acaacatggt atcgacctca
     1021 cttattatcc gaaaattcat acgtcaccga ttctatagtc agcggctgac tcttgtcgac
     1081 gggctctatt ctaatatgga aaaatacata ttcgctgaca ggttcataaa catagcttct
     1141 cctcagagag taatccctgt aagagtactt gcttattgtg aattcaaaag gtgatattcc
     1201 tctactaatg tgctccacgg tactggtgtc gaatatgaag ttgtttatga caatttcaac
     1261 attctccgtt gtgttgatgt tcaaaacaag gttcttgtca tgaacatact ccttcggtac
     1321 acttatctcg taaagggcat aatgcttgga atcatcaaga accacactgt cgcccttgac
     1381 atcttttaca aagctgaagt cctcaagaaa aacaggcaga tatttctcct catcctgcaa
     1441 atccacatat ccgtgcaggc ttacactttg atgaaccaaa gtgttgttgt acgccttggt
     1501 tggttttctg ccatttatct tgacgttcac atcaggtccg tcaacaacaa aacctgcaat
     1561 atgcatctgc ggtgacatgg aaaactcggt ggccccaagt ttttgagaaa gcagctttat
     1621 gagaagggcg gctttcactc cttcttctct acccaactgg gaaatcaagc tgttgatttg
     1681 cgtattgccg taat
//
LOCUS       DAONVY010000497         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_47462_length_1685_cov_2.267485, whole genome shotgun sequence.
ACCESSION   DAONVY010000497 DAONVY010000000
VERSION     DAONVY010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47462_length_1685_cov_2.267485"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1633)
                     /locus_tag="PLI11_10645"
     CDS             complement(23..1633)
                     /locus_tag="PLI11_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53367.1"
                     /translation="MKVRNCILFACIICLCFSIYGVLDNKVNAEESVYPMWYKIFTKH
                     NSTFTPTLVDDTEVKWNEEYKAVELISKESGEYMSWDPYIDYYGLNHSSGDFDGVALE
                     YKPGITIAARVFVPETVVVENPDLEFSLRGLGNDKELLKVSQSVSFRALNGDGSVKKG
                     VWQTVYATFNTTDNVGKEFTGLRISYMKKEGNADMLDSEKTLFIQSICIFDTYDKAIA
                     YYEGGKPVVHTQAYATTNGETTTFNYSVYPVDTKYTVTYSSKTNEEIKDVTTLPTEEG
                     EYLVTFTLEEGTWATGFSSNNTYTTEIKIVSSLPSSEYLVIESEEDVDIWFRDNLNNI
                     PADALSFDSEKKAAKITFNRAVGDPGIYIRGLDVFGEDYPFIAINIEVKGNGSKGGTN
                     NLYVWGEDGLISTLGAGKLKEGSYTVCYDLRIEGSPYQLRWDPFVVIPDGFDVEVNIK
                     YVAFFKSAEAALAFDGDFSVIDNGGGGDEKTPTPDNTTPQEERTPKPLETTTPGEDDK
                     ESKDDSKKSNPLIFVIIGAVVVSQLLWL"
BASE COUNT          481 a          380 c          271 g          553 t
ORIGIN      
        1 tttctctttt tcaaaaccag tattacaacc acagcaactg cgatactaca acagcaccaa
       61 taattacaaa aatgagtgga ttgcttttct ttgaatcatc tttggattcc ttgtcatctt
      121 cacctggtgt tgtggtttcc aatggttttg gcgttctttc ttcctgcggg gtcgtattat
      181 caggagtcgg tgtcttctcg tcaccaccac cgccattgtc aatcactgaa aaatctccat
      241 caaaagccaa tgccgcttca gcactcttga aaaacgcaac atatttaata tttacttcga
      301 catcaaaacc atcaggtatg acaacaaatg gatcccagcg cagctgatac ggagagcctt
      361 ctattctcaa gtcataacaa acagtatagg aaccctcttt taatttgcct gcacctaaag
      421 tagaaattag tccgtcttct ccccaaacat agagattgtt ggttccgcct tttgagccat
      481 ttcctttaac ctcaatatta attgctataa aaggataatc ttctccaaac acatcaagac
      541 cgcgaatata tattccagga tcgcctaccg ctctgttaaa agtaattttt gctgctttct
      601 tctcactatc aaaggaaaga gcgtcggcag gtatattatt caaattatcc ctaaaccata
      661 tatcaacatc ctcttcagac tctataacca gatactcgct cgatggaaga cttgaaacta
      721 tcttgatctc agtagtatat gtgttgttgc ttgaaaatcc tgtcgcccaa gttccttctt
      781 ccaaggtaaa ggtaaccaaa tactcgcctt cttcagttgg cagtgttgtc acatctttga
      841 tttcttcatt tgtcttgctg ctgtatgtaa cagtatattt ggtgtcaaca gggtaaacag
      901 agtagttgaa tgttgtggtt tcaccattgg ttgttgcgta tgcttgtgta tgaacaaccg
      961 gcttgccacc ttcgtaatat gctatagcct tgtcataagt atcaaaaatg catattgatt
     1021 gaataaatag agttttttca ctgtctaaca tatctgcgtt gccttctttt ttcatatatg
     1081 aaatgcgcag tccagtaaac tccttgccaa cgttgtctgt cgtgttaaag gtagcgtata
     1141 cagtttgcca tacgcctttt ttgacagagc cgtccccatt caatgctcta aaagaaacac
     1201 tctgggaaac ttttagaagt tctttgtcat ttcccagacc tcttaatgaa aactccaaat
     1261 caggattctc aaccacaacc gtttcaggga caaacactct tgcagcaata gttattcctg
     1321 gtttgtactc caatgcaact ccatcaaaat caccggatga atggttaaga ccataatagt
     1381 cgatataagg gtcccaggac atatactcac ctgattcctt tgagataagt tctacagcct
     1441 tatactcttc attccatttc acctcagtat cgtcaacaag ggttggtgta aatgtcgaat
     1501 tatgctttgt aaatatttta taccacattg gatagaccga ttcttcagca ttcaccttgt
     1561 tgtcaagaac tccgtaaata ctaaagcaaa ggcatataat acatgcaaat aatatacaat
     1621 ttcgtacttt catcgattga tataccccat ttttctttta ttttctttat tatacatgta
     1681 acctt
//
LOCUS       DAONVY010000498         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_47738_length_1677_cov_3.483970, whole genome shotgun sequence.
ACCESSION   DAONVY010000498 DAONVY010000000
VERSION     DAONVY010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47738_length_1677_cov_3.483970"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLI11_10650"
     CDS             complement(<1..672)
                     /locus_tag="PLI11_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HPZ53368.1"
                     /translation="MKETILILDFGGSHSQLLARKIRKNNVYCEILPYNAAISRIRSL
                     NPKGIVLTGNIVGSNLNEMSLCTEEIFKLSCPVLGINYGALVLTKMLGGSISALNQTE
                     ECAKVLTSLEKKSILFKSIETENYSWLNQTVDIEKVPKGYNITGKTKEGKTCAFENEE
                     KNIYGLLFHPEVSETVSGNEILRAFLYDVCKVSGTWNMKTYVEEIIEDLKEKFQDSKV
                     LLGLSG"
     gene            854..>1677
                     /locus_tag="PLI11_10655"
     CDS             854..>1677
                     /locus_tag="PLI11_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPZ53369.1"
                     /translation="MTEKQNISLDSYRVFCAVAKHKSLTKAAEELYITQPSVSMAISK
                     LEKQLGSKLLTRSAKRVSLTREGEVMYSYLKQAMNLIDTAEEKFWEMQLLKSGEVVIG
                     ASDTLSANYLLPYLQKYFELYPEIQLKVTNRTTSETIELLKNGTVDFGFVNLPIKKDN
                     SLEVYECMHISDVLIGGRMFKHLAKTGVRLEDLNNYHLIMLEKASNTRNYIDDYAQSH
                     NVTLNPVIELGSSEILVKFAGINLGLTFVVKEFYGNHIDNENLFEIPLTPPIPKRS"
BASE COUNT          542 a          348 c          275 g          512 t
ORIGIN      
        1 gcctgacaat cccaaaagaa ccttgctgtc ctggaacttt tcctttaaat cttctatgat
       61 ttcctccaca tatgttttca tattccaagt accagatact ttacatacat cgtaaagaaa
      121 tgcccttaag atttcattgc cgctaacggt ttcagatact tcgggatgaa acaaaagacc
      181 ataaatattt ttctcctcgt tctcaaaggc acaagttttt ccttcctttg ttttgccagt
      241 aatgttataa cctttaggta ctttctctat atcaactgtc tggtttaacc aactgtagtt
      301 ttccgtttca atggatttaa aaagaatgga tttcttttca agacttgtta agacttttgc
      361 gcattcctca gtctgattta aagcacttat tgaccctccg agcatttttg tcaaaaccaa
      421 tgctccatag tttataccca atacaggaca actcagcttg aatatttcct ctgtgcacaa
      481 tgacatttca tttaaattcg aaccaactat atttcctgtc aacactatgc cttttggatt
      541 caatgaccta atcctgctta tggcggcatt gtatggtaaa atctcacagt atacgttatt
      601 cttacgaatc tttcttgcca agagctgact gtgtgaacca ccaaaatcca gaatcaaaat
      661 agtttctttc aagaatatac actcccccaa agatattgct gcaatatttt cactgcaacg
      721 gaataattat attctaaaaa ctattattag taaagttgat atttcttatg ccattcatat
      781 actcagctta tgtttttatt gacatacaaa cttaatattg atattatcag gataaataaa
      841 ggaaggagaa aatatgacag agaagcaaaa catttcactt gacagttaca gagtattttg
      901 cgcagtggca aaacacaaaa gccttaccaa ggcagctgag gagctgtata taactcagcc
      961 atctgttagc atggccattt ccaaacttga aaaacagcta ggctcaaagc ttcttacgag
     1021 atctgccaaa agggtctccc ttacaagaga aggagaagta atgtactcct atctcaaaca
     1081 ggccatgaac ctgattgaca ccgctgaaga aaagttttgg gagatgcagc ttctaaagtc
     1141 aggtgaagtt gtaatagggg ccagtgatac tttgtcagcc aactatctcc ttccctacct
     1201 ccagaaatat tttgaactct accctgaaat acagctcaaa gtaaccaata ggacaacaag
     1261 tgaaactata gagcttttga aaaatggcac cgttgacttt ggtttcgtta atcttcctat
     1321 caaaaaggat aattcactgg aagtgtacga gtgcatgcat atatccgatg tactgatagg
     1381 aggaaggatg ttcaaacatc ttgccaaaac aggagttaga cttgaagacc tgaacaacta
     1441 tcatcttatt atgcttgaaa aggccagcaa cacgcgaaac tatattgacg actatgcaca
     1501 aagccacaat gtaactttaa atcctgtaat tgaactaggc tcaagcgaga ttcttgttaa
     1561 atttgcaggc attaatctag gccttacttt tgttgttaag gagttctatg gaaaccatat
     1621 agacaacgag aatctttttg agataccatt aactccccca ataccaaaaa ggtccat
//
LOCUS       DAONVY010000499         1671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_47991_length_1671_cov_3.113243, whole genome shotgun sequence.
ACCESSION   DAONVY010000499 DAONVY010000000
VERSION     DAONVY010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47991_length_1671_cov_3.113243"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLI11_10660"
     CDS             <1..214
                     /locus_tag="PLI11_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPZ53370.1"
                     /translation="NFEQVDIPKVFEIYNDYVIDRDKDKLKARISDIEINRQPDNCTD
                     CRKCMEKCPQSIDIPNMLSTILALVK"
     gene            295..>1671
                     /locus_tag="PLI11_10665"
     CDS             295..>1671
                     /locus_tag="PLI11_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPZ53371.1"
                     /translation="MFSNVLSKGKIGSVKLENRFVMPAMGSHHGTEDGKVGQKIIDYY
                     AARAKGGFGLIITEFTCVDLPGKALPGQLMITSDEYIEGFKKLTDAIHETKSKIFLQL
                     QHSGRQTNSYITGTQPVAPSAIPCPVNKEIPRALTTEEVYEIIEKFGDAAVRAKKAGF
                     DGVEIHGAHGYLPAQFLSGYSNKRTDEFGGDLVNRARFSTEIIKNIKKKCGSDFPVVF
                     RISGEEKVHGGREIEEAVAIAKLLEAAGADAIHVSLGVYASMPWMVPPANVKAGFNVW
                     AAAAVKKSVNVPVIAVGRINDPMLAETIIENEAADFVALGRAAIADPEFPNKVKENRI
                     NEISPCIGCLTRCNGEPGCDPNDKGVSCMVNPFTGHEGTWILKPAEKAKRVVIVGGGP
                     GGLECAWLSAKKGHEVILLEKSDRLGGQMTPAAVPPNKHEISRAIGYYITMCKKYGVD
                     IRLNTEATA"
BASE COUNT          553 a          310 c          389 g          419 t
ORIGIN      
        1 caattttgag caggttgata tccctaaagt ttttgagata tacaatgatt atgtgattga
       61 cagggacaag gacaagctga aggccagaat ttcagatatt gaaataaaca ggcagcctga
      121 caattgtacc gactgtagaa aatgcatgga aaaatgtcct caaagtattg acattccaaa
      181 tatgttaagt acaatattgg cactagttaa ataagtaaca aacaatttaa ctagaaaact
      241 actgtattaa cataaatatt ctcaaacact atacttagaa ggagatgaaa atgtatgttt
      301 agtaatgttt tatccaaagg aaaaattggc agtgtcaaat tggaaaacag atttgtcatg
      361 cctgctatgg ggtctcatca tggaacagaa gacggcaagg taggtcagaa gattattgac
      421 tactatgcag caagagcaaa gggaggattt ggccttatta ttaccgagtt tacatgcgta
      481 gacttgccag gaaaagccct tcccggacag cttatgatta cctccgatga atatatcgaa
      541 ggtttcaaaa agcttacgga tgcaattcat gaaactaaga gcaaaatctt tttgcagctt
      601 cagcattccg gaaggcaaac caactcatat atcacaggaa ctcaacctgt ggcaccatca
      661 gcaattccat gtcctgtaaa caaggaaata ccaagagcac ttaccaccga agaggtgtat
      721 gaaattattg aaaagttcgg ggatgcagca gtaagagcca aaaaagcagg ctttgacgga
      781 gttgagatac atggcgcaca cggttatttg ccagcacaat tcttgtctgg ttattcaaat
      841 aaaagaactg atgagtttgg cggcgacctg gtcaatagag caagattctc cactgaaatt
      901 ataaagaata ttaagaagaa atgcggaagc gattttcctg tagtctttag aatcagcggt
      961 gaggaaaaag tacacggcgg acgtgaaatc gaggaagcag ttgccattgc aaaacttctt
     1021 gaagctgcag gtgctgatgc aattcacgtt tctcttggtg tttatgcaag catgccatgg
     1081 atggtaccac cagccaatgt aaaggcgggc ttcaacgtat gggctgcagc agcagtcaaa
     1141 aagtcagtta atgttcctgt tattgcagta ggaagaatta atgaccctat gcttgctgaa
     1201 acaatcattg agaatgaggc agctgatttt gttgctcttg gaagagcagc tattgctgat
     1261 cctgaattcc ccaacaaggt taaggaaaac aggataaacg aaatttcacc atgtatcgga
     1321 tgtctgacaa gatgcaacgg agagcctgga tgtgatccta acgataaggg agtttcctgc
     1381 atggttaacc cattcacagg acacgaaggt acctggatct taaaaccagc tgagaaagca
     1441 aagagagttg ttatcgttgg tggaggtcct ggcggtcttg aatgcgcatg gctttctgca
     1501 aagaaaggcc atgaggtaat tctcctcgag aaatccgaca ggctcggagg tcagatgacc
     1561 cctgcggcag ttccgcccaa caagcatgaa ataagccgtg caattggcta ttatattaca
     1621 atgtgcaaga aatatggtgt ggacatcagg cttaacacag aagcaactgc t
//
LOCUS       DAONVY010000500         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_48199_length_1665_cov_4.559627, whole genome shotgun sequence.
ACCESSION   DAONVY010000500 DAONVY010000000
VERSION     DAONVY010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48199_length_1665_cov_4.559627"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLI11_10670"
     CDS             <1..550
                     /locus_tag="PLI11_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53372.1"
                     /translation="FRENFEALNQYGMSRKTIYKGKVLAYSSLALAAAIIDRIIVIAA
                     SKITSSLENFIFGSFFTTMYESFFDKLNFWSENLLALLLSFSAYLALMSFGTFLSAVF
                     YRSNRIGRIAIAAGVPIFIFVALPIIAAYVFTTNLANLITKFIDFTLSTPLRTSATCL
                     LGAIILGVISYPIIVRATLKGK"
     gene            656..1588
                     /locus_tag="PLI11_10675"
     CDS             656..1588
                     /locus_tag="PLI11_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPZ53373.1"
                     /translation="MKHVSINSTDLTVSNICLGTGRFGNSLDASSSKQQLSHFVYSGY
                     NFIDTARVYGNSEKVIGEWLKETSLRSSVVISSKGAHPDHDWNPRMTKKDIQFDIETS
                     LATLGTDYIDLYFLHRDDRNVPVCEIIDILEELCNSGKIRYYGCSNWTLERIIEAQQY
                     AKKSNAKGFVCNQVMLSLTKANKNVFEKMDMVQADKDMVEYHKNTNLALMSYMTLAGG
                     YFIKLMNNSEINENCKAMYDNEENRKTLVVIKDLCKSYGYSPLELLLHYVLARPFCAL
                     PVISFSSIGQMDEVLRHTERSIQKEHMDMLLEII"
BASE COUNT          515 a          330 c          335 g          485 t
ORIGIN      
        1 cttcagggaa aactttgaag cactcaacca atatggtatg agcagaaaga ccatatacaa
       61 aggaaaagtg ctcgcataca gttcccttgc actggctgcg gccattattg acagaattat
      121 tgtaattgcc gcatcaaaaa ttacatcctc attggaaaat ttcatatttg gaagcttttt
      181 taccacaatg tatgaaagct tttttgacaa actaaatttc tggtccgaga acctgctcgc
      241 actcctccta tccttctctg cttatcttgc actaatgtct ttcggaacat ttttatccgc
      301 tgttttctac agatcaaatc gcataggcag gatagctata gctgcaggag tgccaatatt
      361 tatctttgtt gcattaccca ttattgcagc atatgtcttt acgaccaatt tagccaattt
      421 gattacaaag tttattgact ttactctgtc tactccttta agaacttctg ctacgtgttt
      481 gctcggtgca attattctcg gggtgatatc ctacccaatt attgtgcggg caacgttgaa
      541 aggaaaatag agaataaaag ccgcgcaaaa agcgtggctt tctcttttta ttgacaattg
      601 tatggagttg tcatattatt attagaccca taatatttac attggatggt attgaatgaa
      661 acatgtcagc attaattcaa ctgatttgac agtatcaaac atctgtcttg gaaccggcag
      721 gttcggaaac agccttgatg catcatcaag caagcagcag ctttctcatt tcgtatattc
      781 cggttacaat tttattgata ccgcccgggt ttacggaaac agcgaaaaag taatcggcga
      841 gtggctaaag gaaaccagcc tgcgctcctc cgttgtgatt tcttcaaagg gtgcacatcc
      901 ggatcacgac tggaatccca ggatgacaaa aaaagatatt caatttgata ttgaaaccag
      961 ccttgccact ttgggaaccg attacataga tttgtacttt ctgcacaggg atgacagaaa
     1021 tgtaccggta tgcgaaataa ttgacatttt ggaagagctg tgcaacagcg gaaaaatacg
     1081 ctactatggt tgctccaact ggacccttga aaggataatc gaggcacagc aatatgcgaa
     1141 aaaatccaac gccaagggtt ttgtctgcaa ccaggtcatg ctgtccttga caaaagcaaa
     1201 caagaatgtt tttgaaaaga tggacatggt tcaggctgac aaggatatgg tggaatacca
     1261 caagaatacc aaccttgccc ttatgtcgta catgactctt gccggagggt attttataaa
     1321 acttatgaat aactcggaga ttaatgaaaa ctgcaaagca atgtatgata acgaggaaaa
     1381 ccgcaaaaca ttagtagtaa taaaagattt gtgcaaaagt tacggataca gcccccttga
     1441 acttttgctg cactatgtcc ttgcaagacc cttctgcgcc ttgcctgtca tttccttttc
     1501 aagcattggg cagatggatg aggtattaag gcacaccgaa agaagcattc aaaaagaaca
     1561 catggatatg ctgttagaga ttatttaggt ttgaacaaag aaaggatttt tatggagttc
     1621 aacattgatg tttttacaga ctatctatca aagtacggag tcatt
//
LOCUS       DAONVY010000501         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_48252_length_1664_cov_3.510876, whole genome shotgun sequence.
ACCESSION   DAONVY010000501 DAONVY010000000
VERSION     DAONVY010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48252_length_1664_cov_3.510876"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLI11_10680"
     CDS             <1..564
                     /locus_tag="PLI11_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HPZ53374.1"
                     /translation="QYVVMALLLVIIVVRHKKHNIIDAAITVMGGYYVVFLSSFFLLL
                     RYKESGVFLFFIALVGTVSSDTAAQVFGVKWGKKKLIPEVSPKKTVIGSFGAFVGPLI
                     FVVLYGYIINRLGYVNIKLYHFVILAILLGAGAQIGDLAASAMKRYANVKDFGKLIPG
                     HGGVLDRIDSLLFCVPMVYYYLRFISI"
     gene            576..>1664
                     /locus_tag="PLI11_10685"
     CDS             576..>1664
                     /locus_tag="PLI11_10685"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPZ53375.1"
                     /translation="MVKTVSIIGSTGSIGTQTLEVIRELNEKGADIRVIALSAGNNVG
                     LLLEQAREFNVKYLSIVREKDAETLRENLRDINPEVYSGDEGLNRIAEIKTDLLFTAI
                     VGMRGLLPTITAINAGNNIALANKETLVSAGSIVMNLAKEKGVIIFPVDSEHNAILQC
                     LDAGQKKDVKKLILTASGGPFRAFTKEDMYKVSVEDALNHPTWNMGGKITIDSATLMN
                     KGLEVIEAMHLFDMDAEDIEVVVHPQSIIHSMVCYKDNSVIAQLSMPSMKLPIQYALT
                     YPDRMESRIPQVSFAEIGTLTFEEPDLDRFPCLKYAYEAARTGGTMPCVMNAANEVCA
                     ESFLRGEIPFMTISEIVKKVMEGHKSLPS"
BASE COUNT          502 a          282 c          423 g          457 t
ORIGIN      
        1 cagtatgtgg ttatggcctt gctgttggta attattgtag taagacacaa aaagcacaat
       61 ataatagacg ctgccataac cgttatggga ggatactatg tggtgtttct atcaagcttt
      121 ttccttttgc tcagatacaa ggagtcggga gtgtttttgt tctttattgc actggtggga
      181 actgtatctt ccgacacagc agcccaggta ttcggggtga agtggggaaa gaaaaagctc
      241 attcctgagg tcagccccaa gaaaacggtt attggaagct ttggagcatt tgtaggtcca
      301 ttgatttttg tagtattgta cggctacata ataaacagac tgggatatgt aaatattaaa
      361 ctgtaccatt ttgtaattct ggccatactt ttgggagcag gtgcacagat tggagatctg
      421 gcggcatctg caatgaagag atacgcaaat gttaaagatt ttggtaagct cataccggga
      481 catggtggag tactggacag gattgacagt cttttgttct gtgtaccaat ggtatactac
      541 tacttgagat ttataagcat ttagaaggag aacatatggt aaagaccgtt tcaatcatag
      601 gatcgacggg ttccatcggg acccagaccc ttgaggtgat aagggaactg aatgaaaagg
      661 gagcggatat tcgtgtaata gcgctcagtg ctgggaacaa tgtaggcttg cttttggaac
      721 aggcaaggga gtttaatgta aagtacttat cgatagtcag ggaaaaagat gcggaaacct
      781 taagagagaa tctgagggat ataaatccgg aggtatattc cggtgatgaa ggattaaata
      841 gaatagctga aatcaagacg gatcttctct ttactgcaat agtgggaatg cgcggacttc
      901 tgcccacaat aaccgccata aatgcaggca acaatattgc actggccaac aaggaaactc
      961 ttgtcagtgc aggcagtata gtaatgaatc ttgcaaaaga gaaaggtgtt ataatttttc
     1021 ctgttgacag cgagcataat gcaatactgc agtgtctgga cgcaggacag aagaaggatg
     1081 tgaagaaact catacttaca gcatcgggag gccctttcag ggcttttaca aaagaggata
     1141 tgtataaagt gtccgtggag gatgctctta atcatcccac ctggaatatg ggaggaaaga
     1201 ttaccattga ttccgcaacc cttatgaaca agggacttga ggtaattgag gctatgcatc
     1261 tttttgacat ggatgctgaa gacatagagg ttgtggtaca tccgcagagt attattcatt
     1321 ccatggtatg ttacaaggat aacagtgtca ttgctcaatt aagcatgcca agcatgaaat
     1381 tgcctatcca gtatgcgttg acttatcctg acaggatgga gtcacgtata ccgcaggtat
     1441 cctttgcaga gattggtact ctaacctttg aggagcctga tttggataga ttcccatgcc
     1501 tcaagtacgc atatgaagct gcaaggacag gtggtaccat gccttgtgtt atgaatgcag
     1561 caaatgaagt atgtgcggaa agttttctaa gaggcgaaat cccgtttatg acgatttccg
     1621 agattgtaaa aaaagttatg gaaggacata aaagtttgcc aagt
//
LOCUS       DAONVY010000502         1662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_48332_length_1662_cov_4.286870, whole genome shotgun sequence.
ACCESSION   DAONVY010000502 DAONVY010000000
VERSION     DAONVY010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48332_length_1662_cov_4.286870"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1464
                     /gene="ypeB"
                     /locus_tag="PLI11_10690"
     CDS             112..1464
                     /gene="ypeB"
                     /locus_tag="PLI11_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HPZ53376.1"
                     /translation="MKARNFVASTMVFLMLVGLTVYGVYYNKMAQDYEIALENQYNRA
                     FTDLVEHTNNVEEYLLKALATGTPQKRAQLLENAWNSATQAAACLSMLPMNHNMMSKV
                     QNFLVQIGDISYSWNNGAIRGEKITDDDYKTLTQLYGYATELNSGLFEVYSDFTSQRY
                     NWRDIQKDSEKVFSDDTVLEKYESLSKLTKPFENYPALIYDGPFSTHMSDIKPKGLKG
                     DNITKEQGADKIYKYFSDRKPTVNIAENNRNANIKTYSYTVSFEEEDIHVAYVDLTEV
                     GGMVYYYLLYRDIGEPNLSAEQATQIGKDFLARLGVVDMEPSYHIIEDNVATINYVYV
                     KEGVRYYPDMMKVKVALDTGEVIGLESHAYITSHHDRKTPTPKITAEEAQEKVSPLLT
                     IEDVNKAVIPNDFGGEHFVYEFKCNKDDRNFLVYIDAEDGSEVEVLILLENENGVLTV
                     "
BASE COUNT          562 a          283 c          375 g          442 t
ORIGIN      
        1 agaattcttt ccttccaatt tttttaagga taaaatgcaa gttttagtta aaaatacaat
       61 tgaatcaagt ttaggtaaaa caaagcttaa gaagcggaat tggaggagaa tatgaaggca
      121 agaaactttg tagcgtcaac aatggttttc ttaatgctag tagggcttac agtatatggc
      181 gtctattaca acaaaatggc ccaagactat gaaattgcac tcgaaaacca gtacaaccgt
      241 gcctttaccg accttgtaga gcatacaaac aatgtggaag aatacctgct caaagcattg
      301 gcaacaggca ctccgcaaaa gcgtgcccag cttttggaga atgcctggaa cagtgccaca
      361 caggcggctg catgtctgag catgcttcca atgaatcaca acatgatgtc aaaagtacag
      421 aattttctgg ttcaaatagg tgacatatcc tattcgtgga acaatggagc aataagagga
      481 gaaaaaataa ctgatgatga ttacaaaacc ctgacgcagc tctatggtta tgcaacggag
      541 cttaactcag gtcttttcga agtatattct gattttacat cccaaaggta caactggcgg
      601 gatattcaaa aggattctga gaaagttttt tcagatgata cagtgttgga aaagtatgaa
      661 tccttgagca agctgaccaa accctttgaa aactaccctg cgctcattta cgatggtcct
      721 ttctcaaccc acatgtcgga tatcaaacca aaaggtttga aaggcgacaa tataacgaag
      781 gaacagggag cggataaaat ctacaaatat ttcagtgaca ggaagcctac tgtcaacatt
      841 gcagagaaca acaggaacgc aaatatcaaa acatacagct acactgtaag ttttgaggaa
      901 gaagacattc atgttgcgta tgttgacctg acagaagttg gcggtatggt ttactactat
      961 ctgctgtata gggatattgg tgaaccaaac ctgagtgctg aacaggcaac gcaaataggg
     1021 aaggattttt tggcacgact tggagttgtg gacatggaac cttcctatca tataattgaa
     1081 gacaacgtag ctacgatcaa ctatgtttat gtaaaggaag gagttcgcta ctatccagac
     1141 atgatgaagg tgaaagttgc tttggacaca ggagaggtaa ttggtcttga aagccatgcc
     1201 tacatcacta gccatcatga caggaaaact ccgactccaa agattacagc cgaagaagcg
     1261 caagaaaaag taagtccttt actgacaatt gaggatgtga ataaggcggt aattcctaac
     1321 gactttggtg gcgagcattt tgtttacgag tttaaatgca acaaggacga tagaaacttc
     1381 cttgtatata tagatgcgga ggatggaagt gaggttgaag tccttatact tcttgaaaat
     1441 gaaaacggtg tccttacagt ttaaaaaatt ggaacctata tgtttagtat aggttctttt
     1501 tttgtctttt tggccggtat aaatgaaaag gggcgataat agaatatata ataataatgt
     1561 gatataattg ggggtagtcc aagtgggttt gttcagcaga ctgaagagtt tgttcggagg
     1621 tttttttgga gtaatgactg gaaaaataga gaaaaataac cc
//
LOCUS       DAONVY010000503         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_48398_length_1661_cov_2.777086, whole genome shotgun sequence.
ACCESSION   DAONVY010000503 DAONVY010000000
VERSION     DAONVY010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48398_length_1661_cov_2.777086"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1661)
                     /locus_tag="PLI11_10695"
     CDS             complement(<1..>1661)
                     /locus_tag="PLI11_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HPZ53377.1"
                     /translation="ASFNYVVGKIEVNRRGYAFVIPEDKASWSESIYVNSKDVNGAMT
                     GDKVVVSIKNPKKPAFPGEKKEGVVKSVLVKGSRTFSGVVKWNPIRHGLMVLADDEYW
                     YIEGNADSLDEGLSVICEALEKGEFPEPGVCRIVEVVGNISDPNTGITTILSLHGFVE
                     EFPDDVMKEASMLPQELDEETIQSELKKGRKDIRGLLTVTIDSEDTKDVDDAITIEIL
                     DNGNYMLGVHIADVAHYVKEGSAIDREAYKRGTSVYPVGRVVPMLPKELSNGICSLNK
                     NCDRLALSVVMEVDEMGNVVDHEIYESILNVDYSITYDQLYRIFEKPDFAILKEFGDY
                     LSQLTLMKNLAYILNERRRRRGAIDFNLPETKVELDDEGRPISVSAYRTTFANNIIEE
                     FMLLCNETVAERFYWYNVPFIYRVHDEPEDEKMIMLSKIVTNLGYKFKNTSKIHPKSI
                     QSLLDSIKDKPEERIIGTMTLRSMQKAAYRSTNDGHFGLALKYYSHFTAPIRRYPDLF
                     IHRIIKHMLHGGMNHKLEERYAKFAGEYAKHLSDRERAAQEAERE"
BASE COUNT          421 a          430 c          290 g          520 t
ORIGIN      
        1 cattcccttt cagcttcttg agctgccctc tcgcgatcag acaaatgctt tgcatactca
       61 cctgcaaatt tggcataccg ctcttcaagc ttgtggttca tgccgccatg cagcatatgc
      121 ttgattatcc tgtgtataaa caagtcggga taccgtctaa taggggcggt aaaatgtgaa
      181 taatatttaa gtgcaagtcc aaagtgtccg tcattggttg atctgtatgc cgccttctgc
      241 atggacctta atgtcattgt tccaattatc ctctcttcag gtttgtcctt tattgagtca
      301 agaaggcttt gtatgctctt tggatgaatc tttgatgtgt tcttgaactt gtacccaaga
      361 ttggtcacaa tctttgacag cataatcatc ttttcgtcct caggttcgtc atgcaccctg
      421 tatataaagg gcacattgta ccagtaaaac ctctcagcaa ccgtctcatt gcaaagaagc
      481 atgaactcct cgattatgtt gtttgcaaag gttgtcctat atgccgacac ggaaattggc
      541 cttccttcat catccaactc aactttggtt tccggcagat taaaatcaat tgctccgcgt
      601 cttcgtcttc tttcattcaa tatatatgca aggttcttca taagagtcag ctgactcaga
      661 taatctccaa actctttcaa aatcgcaaaa tcaggctttt caaatatcct gtacaactgg
      721 tcataggtaa tggaataatc cacattgagt atgctttcgt aaatctcatg gtccaccaca
      781 ttgcccattt cgtccacttc catcacaacg gacaaagcaa gcctgtcgca gtttttattc
      841 aaactgcata tgccgtttga caactctttg ggcagcattg gaacaaccct tcccacagga
      901 taaacgcttg ttcccctttt ataggcttcc ctgtctatag cggatccttc cttaacatag
      961 tgggctacat ccgctatatg gacccccaac atataattgc cgttatccaa aatttcaatg
     1021 gttatggcgt catccacatc cttggtgtct tcagagtcaa tagtgactgt taaaagcccc
     1081 cttatatcct tcctgccttt cttaagttca gactgtattg tttcttcatc caattcctga
     1141 ggcagcatgg aagcttcctt cattacatca tccggaaact cctccacaaa gccgtggaga
     1201 ctcaaaatcg tggtaatacc agtgttcgga tctgaaatat tgccgacaac ttctactatt
     1261 ctgcaaaccc ctggctcagg aaactctccc ttttcaaggg cttcacatat gacactgagt
     1321 ccttcatcca aggaatccgc attcccttca atataccaat attcgtcatc agccaaaacc
     1381 attagcccat gacggattgg attccatttt acaactcccg agaaggttct ggagcccttg
     1441 acaagtacac tcttgacaac gccttccttt ttctcacccg gaaacgcagg ctttttggga
     1501 ttcttgatgg agactaccac tttatctcct gtcatggcgc catttacatc cttggaattg
     1561 acgtatatgc tttcgctcca gctagccttg tcctcgggta tgacaaaagc atatcctctc
     1621 ctgtttactt ctattttacc aacaacgtaa ttgaaactgg c
//
LOCUS       DAONVY010000504         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_48744_length_1653_cov_2.403004, whole genome shotgun sequence.
ACCESSION   DAONVY010000504 DAONVY010000000
VERSION     DAONVY010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48744_length_1653_cov_2.403004"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1316
                     /locus_tag="PLI11_10700"
     CDS             66..1316
                     /locus_tag="PLI11_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53378.1"
                     /translation="MKTKRLLFINVLLVALILCLSISASYAFDTNMDGWTSAGNGVWE
                     VKEDGLYATNNNGNSLAKSNVVIDGTKSFIYEVTAEFTGHGFGIAFGVVDNENFTAVE
                     INTEGLIYCPRRVNNEWSLWAKDFKPLPGGVDGSDGYTIKFMFDAVDKSAYIFVNGQL
                     AETITDINVDLIKGNLGLQTEVSDAVVTKATYTEVESINLFETNISGWTSAGSGSWEK
                     KNIGLLANNNGGNNLCKTDLVIDGTKSFEYEVTGVFPGHGFGIAFGVVDDENFTAVEI
                     NTEGKLYCPRRVDNEWSLWANDFKDLPEGVNGTDGYTIKFVFDAEEKSAEIYVDGILA
                     DTISDIDIELIKGNVGLMTEHSDVIVTKAVFIDLSVEPSPPPTEENVETGDYTGSNIV
                     LLLMIMMTALSALIVFKVKIAEKA"
     gene            1459..>1653
                     /locus_tag="PLI11_10705"
     CDS             1459..>1653
                     /locus_tag="PLI11_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPZ53379.1"
                     /translation="MTSIGIDIGTTSISAVLVEINSGKVLKSITVSNKTDISSNGEEH
                     LQDPEKIFNKVRAIIDEIIEF"
BASE COUNT          526 a          219 c          392 g          516 t
ORIGIN      
        1 tacacttgtt gggggtatgt tgaatttata tattcatatc agtattatta tatggaggta
       61 tctaaatgaa aacaaaaagg ttgttgttta ttaatgtact gttggttgca ttaatccttt
      121 gtctatccat tagtgcatca tatgcttttg atacaaacat ggatggctgg actagtgcag
      181 gtaatggagt atgggaagta aaagaggatg gtctttatgc aacgaacaat aacggtaaca
      241 gtttggcaaa atccaatgtc gtgattgacg gaacaaaatc ctttatttat gaggtaacag
      301 ctgaatttac tggacatgga tttggaatag cttttggcgt agtggacaat gagaacttta
      361 cggcggttga gattaatact gaaggtttga tttattgtcc gagacgtgta aacaatgaat
      421 ggtctctctg ggcaaaggat ttcaagcccc tgcctggtgg agtggacggc tctgatggct
      481 acacaataaa atttatgttt gatgctgtag acaagtctgc ctatatattt gttaatggtc
      541 aacttgcaga aacaataaca gatataaatg tggatttgat taaaggtaat cttggtctgc
      601 aaaccgaagt ttccgatgct gtcgttacaa aagcaacata tacggaagtt gagtcaatta
      661 atctttttga aaccaatatt tcaggatgga caagtgctgg aagcggaagt tgggaaaaga
      721 agaatattgg attgttggca aacaacaatg gaggtaacaa tctgtgcaaa accgaccttg
      781 taattgacgg tacgaagtct tttgagtatg aggttacagg ggtatttcct ggacatggct
      841 ttggtatcgc atttggtgta gttgatgatg aaaactttac cgcagtggaa atcaacactg
      901 aaggcaagct ttattgtccg agacgtgtag acaatgaatg gtctctttgg gcaaacgatt
      961 ttaaagacct tcctgaaggt gtgaatggaa ctgatggtta cacaatcaag tttgtgtttg
     1021 atgcggaaga aaaaagtgct gaaatttatg ttgatggaat acttgctgat acgatatctg
     1081 atattgatat agagttgata aagggtaatg tcggcttaat gaccgagcac tccgatgtta
     1141 tagtcacaaa ggctgtattt attgatttgt cagtggaacc atcaccacct ccaactgagg
     1201 agaatgttga gacaggcgat tatactggtt cgaatattgt attgttgtta atgattatga
     1261 tgactgctct gtcagcgctg attgtcttta aggttaaaat tgctgaaaag gcgtaagata
     1321 gtataagagc gtatcgaaaa gatatgctct tataaattct atatccttta gaatatgaag
     1381 gaggtttttg catgaaaaaa ataatataaa gacaaggttg ttgtaacaag ttttatgtat
     1441 tattttgggg aggagtttat gacatcgatt ggtattgata ttggcacaac ttcaataagt
     1501 gcagtcctgg ttgaaattaa tagtggaaaa gtattaaaga gtatcacggt atccaacaaa
     1561 accgacattt cttcaaatgg ggaggaacat ttgcaagatc ccgagaagat atttaataag
     1621 gtaagagcta taatagatga gattattgag ttt
//
LOCUS       DAONVY010000505         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_48850_length_1650_cov_3.177429, whole genome shotgun sequence.
ACCESSION   DAONVY010000505 DAONVY010000000
VERSION     DAONVY010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48850_length_1650_cov_3.177429"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1038
                     /locus_tag="PLI11_10710"
     CDS             163..1038
                     /locus_tag="PLI11_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HPZ53380.1"
                     /translation="MIDVVGVRFRPNGKIYYFNPKGQKFDKGTYVIVETSRELEYGEV
                     VIPNRMVDDEEIVPPLKDVVRVATEEDHETHLKNIEKAKRAHGICLDKINEHGLAMKL
                     IDCEYTFDNNKLLFYFTADGRIDFRELVKDLAAIFKTRIELRQVGVRDEAKTIGGLGV
                     CGRVLCCTSCLGEFQPVSIRMAKEQGLSLSPNKISGTCGRLMCCLNYEQDAYEQALSV
                     VPGVGAVVETPDGNGVVVSSNLLKENVQVRLDKENETELTTYSVSEIKVIRDADVCDV
                     DNFKLGLQDLEEDLD"
     gene            1092..1262
                     /locus_tag="PLI11_10715"
     CDS             1092..1262
                     /locus_tag="PLI11_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPZ53381.1"
                     /translation="MAYHITDDCIACGACVPECPTDCISEGDIYVIDAEQCIDCGSCA
                     AVCPTDAPKPQD"
     gene            1342..>1650
                     /locus_tag="PLI11_10720"
     CDS             1342..>1650
                     /locus_tag="PLI11_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPZ53382.1"
                     /translation="MHPDERIEDLMIGNLRIIQKNDSFRFGTDSILLSLFAELKGKQR
                     VLDIGTGSGIIPILLSYKNLESLFAGLEIQDHIADMAARSVSLNNLNDRISIVRGDVK
                     N"
BASE COUNT          504 a          275 c          427 g          444 t
ORIGIN      
        1 caggttacga taaggagctt tacggccttg cacgcaaact aggctaccat aaagccaagg
       61 agtgcattga agttgttgat gctacgtgga aacgcatagg aagaaatgtc aacttcaagc
      121 tgtcttgcga acttatgttg gttaaactac aggaggtcat taatgattga tgtagtagga
      181 gtaagattca gaccgaatgg taagatatat tattttaatc ccaaaggtca aaagtttgac
      241 aaagggacat atgtaattgt tgaaacttcc agggaacttg agtatgggga agtggtcatt
      301 ccaaacagaa tggtggatga tgaggagatc gttcctccgc tgaaggatgt ggtccgtgta
      361 gctacggaag aagaccatga aacacatctt aagaatattg agaaggcaaa aagagcacac
      421 ggcatctgcc ttgacaagat taacgagcac gggcttgcaa tgaagctgat agactgcgag
      481 tatacctttg acaataacaa gcttctcttt tatttcactg cagatggacg aatagatttc
      541 agagagcttg taaaggatct tgcggcaata tttaaaaccc gtattgagct caggcaggta
      601 ggagtaagag acgaagccaa gaccattgga ggccttggag tgtgcggaag agttttgtgc
      661 tgcacttctt gccttggtga gtttcagccg gtgtccataa gaatggcaaa ggagcaggga
      721 ctttccctga gtccaaacaa aatttcgggc acttgcggta gactcatgtg ctgcctgaat
      781 tacgagcagg atgcttacga acaagcgttg tccgtagtgc ccggggtagg ggcggtggtg
      841 gaaactcctg acggtaatgg agttgttgtt tcttccaatc ttctaaagga gaatgttcag
      901 gtaaggctgg acaaggaaaa cgaaaccgag ctcactacat attcggtttc tgaaatcaag
      961 gttatcaggg atgcggatgt ttgcgatgtg gataatttca aacttggttt acaggattta
     1021 gaggaagacc ttgactaaaa cgcaattggt ggtaaaataa aatagtacgt aaattatttg
     1081 ggggtgtatt tatggcatat catattacag atgactgcat tgcatgcgga gcgtgcgtgc
     1141 cagagtgtcc aacagattgc atttcagagg gtgatattta cgtaatagat gctgagcaat
     1201 gcatagattg tggtagctgc gcggctgtat gccctactga tgctccaaag ccacaggatt
     1261 aatttgtaca tgactttgga ataatggata aaaaagtttg cttggtttca agcaagcttt
     1321 tttgattaaa ggggtttttt tatgcatccg gatgaaagaa tagaagactt gatgattggc
     1381 aatttaagaa taatacagaa aaatgacagt tttagatttg gtactgacag catccttttg
     1441 tcgctttttg cagaactgaa gggaaaacaa agagttcttg acataggaac cggatcagga
     1501 atcatcccca tacttttgtc atacaagaac ttggaaagct tatttgcagg tcttgagata
     1561 caggaccata ttgccgacat ggcagcaaga agcgtcagtt tgaacaattt gaacgacagg
     1621 atttccattg taaggggaga tgtcaaaaac
//
LOCUS       DAONVY010000506         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_49019_length_1646_cov_2.327467, whole genome shotgun sequence.
ACCESSION   DAONVY010000506 DAONVY010000000
VERSION     DAONVY010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49019_length_1646_cov_2.327467"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLI11_10725"
     CDS             <1..185
                     /locus_tag="PLI11_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPZ53383.1"
                     /translation="DAIVEGVADDGIFYYGRTYAEAPEIDGYVYFTSPEPLEFGEVVK
                     VKILVADNYDLTGEVV"
     gene            232..834
                     /gene="pgsA"
                     /locus_tag="PLI11_10730"
     CDS             232..834
                     /gene="pgsA"
                     /locus_tag="PLI11_10730"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPZ53384.1"
                     /translation="MNLPNKITIARIIMIPVFMLFAFPLPAWFPGEKFFSGRYSALIA
                     LILYIIASVTDTVDGHIARKYNLITSFGKFLDPIADKLLVMAAMLALVPMNKMYLWIA
                     MIILTREFIVTGVRLVAASEGVVIAAGKGGKLKMVLQTIALITLLASKLFEGTTGLAE
                     TIGAVVIVFGDVMLIAAAIVTIVSGFEYVKNNLSLIAKDM"
     gene            1036..1581
                     /gene="fapR"
                     /locus_tag="PLI11_10735"
     CDS             1036..1581
                     /gene="fapR"
                     /locus_tag="PLI11_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HPZ53385.1"
                     /translation="MAGKSDKRQKRHKLLIEKLKQDPFLTDSELAAYLNVSVPTIRLD
                     RMHLNIPELRERMMELATDATLPDKSEKYGEIIDLKSGSSGISVMVTTKKMCFDERDM
                     VRGCCLYSMAESLAMTIIDEPAALIGVANIKYKVLVTSDTRLVARGEVRVKRENSFIV
                     WVKIYNDQVEVFSGKFILNKE"
BASE COUNT          532 a          258 c          370 g          486 t
ORIGIN      
        1 atgatgcaat tgtggaaggt gttgcggatg acggcatatt ctattacggt agaacatatg
       61 ctgaagcacc tgagatagac ggatacgttt acttcacttc accggaacct cttgaatttg
      121 gagaggtcgt aaaggtaaaa atattggttg cggacaatta tgatttgacc ggtgaggttg
      181 tttgatatag aattaagtta aaacatttac caaaagatag gaaggctaaa aatgaatcta
      241 cccaacaaaa ttaccattgc aagaattatt atgattccag tattcatgct ttttgcattt
      301 cctttacctg catggtttcc tggagaaaag tttttctcag gacgctattc agcgttgata
      361 gccttgatac tatacatcat tgcatcagtt acagacacgg tggatggtca catagcaagg
      421 aaatacaatt tgataacgag ttttggaaaa tttcttgacc caatcgctga taaacttctt
      481 gtaatggcag caatgcttgc tttggtgccg atgaataaaa tgtatctttg gatagctatg
      541 ataatactta caagagagtt tattgtaact ggtgtcaggc ttgttgcagc gagcgaaggt
      601 gttgtaatag cggcaggcaa gggcggcaag cttaaaatgg tgttgcagac aattgcactt
      661 ataacccttc tcgcatcaaa attgtttgaa ggtacgacag gactggctga aacaataggg
      721 gcggtagtaa ttgtgtttgg tgatgtaatg ctgattgcag cagccattgt tactattgtt
      781 tccggatttg aatatgtgaa aaacaattta agtttgatag caaaagatat gtaagtttgg
      841 aaaaggcttc aacacctgaa gctttttccg tatttttttc atttagtcat aatttttcat
      901 tcccaatcat aaaaaatcct aaaaattgct cttgcattcc tctgtaaagt gagatataat
      961 ctgtttatta ttatcacatg gtaataattt tctaatatta ttatatatat agcatatcac
     1021 tatggaggtg cacagttggc tggtaagtca gataaaagac agaaaagaca taagttgttg
     1081 atagaaaaac tcaagcagga tccatttctt acagattctg agcttgccgc atatttgaat
     1141 gtaagtgtac caaccattag acttgacaga atgcatctca atattcccga gctaagggaa
     1201 aggatgatgg aacttgccac ggatgcaaca ctacctgaca aatcagagaa atatggggaa
     1261 ataatagacc tgaaatcagg tagctccggt atatcggtaa tggtgaccac caaaaagatg
     1321 tgttttgatg agagggatat ggtcagaggt tgctgcctgt acagcatggc ggaatcactt
     1381 gccatgacaa tcatagacga acctgcggca ttgataggag ttgccaacat aaaatacaag
     1441 gtactggtga cttcagatac aagactagtt gctcgtgggg aagtaagagt caaaagagaa
     1501 aacagcttca ttgtatgggt taaaatatat aatgaccaag ttgaagtttt tagcggaaaa
     1561 tttatactta ataaagaata gttgggtgag gagagttaat gagagtactt gtagatgcaa
     1621 tgggcggaga caatgctccg caagct
//
LOCUS       DAONVY010000507         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_49499_length_1634_cov_2.523749, whole genome shotgun sequence.
ACCESSION   DAONVY010000507 DAONVY010000000
VERSION     DAONVY010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49499_length_1634_cov_2.523749"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..363
                     /locus_tag="PLI11_10740"
     CDS             145..363
                     /locus_tag="PLI11_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53386.1"
                     /translation="MKRVVVFLALLFSLFVLTGCFSMVKNNGTGPTPTPEVTEDPDAG
                     KYKEYVNEEYSFKAMYPSDWTISYSVQA"
     gene            420..722
                     /locus_tag="PLI11_10745"
     CDS             420..722
                     /locus_tag="PLI11_10745"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HPZ53387.1"
                     /translation="MDEGIGAHYTLEEYADLAIDEMSSYIENYEQVDFDEFETDNWKG
                     YYLIYTGTYQEFEIAWAQVFFMENDNIYILTFATTVDEYETYVPAAEYIAENFVLQ"
     gene            741..>1634
                     /locus_tag="PLI11_10750"
     CDS             741..>1634
                     /locus_tag="PLI11_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPZ53388.1"
                     /translation="MMDGLVDFILEVEEEKNLRILQITDTQVIDASQSRHEDRLNSVE
                     KIIWATNTMDVNCFNYIRDLVHRTKPDLILITGDLVYGEFDDLSTSWEKYVKFMDGFN
                     IPWAPIFGNHDNESIKGVDWQCEIIEKASYSLFKRGNVTGNSNYTIGIVQNSKFKRVI
                     YMMDSNGCGNTSDPSVKKTVGFALDQLTWFDETANRIESFAGKVKKFVCYHIPTLEFA
                     LAATNAGYQKELYDNAAYTIGEDVPAKNSDFGRKGEIFKGLHEVPDLLALFKKHSVDG
                     VFVGHSHCNSISVLYEGIRWTF"
BASE COUNT          543 a          263 c          370 g          458 t
ORIGIN      
        1 ttacaaacat tctgcataca atacgtaata taaattttac aacaatttaa cattttaaac
       61 atatatgccc ttcaatttgt gtattgtttg tttttcccaa aagatgtata atagctgaac
      121 catataaaaa agtaggtaga aaccatgaag agagttgtag tatttttggc actgctattt
      181 agcctgtttg tattaaccgg ctgtttttct atggtaaaga ataatggtac tggtcctacg
      241 ccgacccccg aggttacgga agacccggat gcaggaaaat acaaggagta cgtaaatgag
      301 gaatattctt tcaaggccat gtatcccagc gactggacaa tcagttacag cgtacaggcg
      361 taatagttac tttctatgca cctgatgatg aaggttttag aacgaatata aacatattgg
      421 tggatgaagg aattggagca cactacaccc ttgaagagta tgcagacctt gccatagatg
      481 aaatgtccag ttacattgag aattatgagc aggtggattt tgatgaattt gaaacagaca
      541 actggaaagg gtattatcta atatacacag gtacatatca ggaatttgag attgcctggg
      601 cacaggtatt ctttatggag aatgacaata tctacatatt aacctttgca acaactgtag
      661 atgaatatga aacgtatgtg cctgccgcag aatacatagc agaaaatttt gtattgcagt
      721 aaagaagtga tactttaaaa atgatggatg gtttggttga ttttatattg gaagttgaag
      781 aagaaaaaaa cctaaggatt cttcaaatta ctgataccca ggttatcgat gcatcccagt
      841 caaggcatga agacagatta aacagtgtgg aaaagataat ctgggcgacc aatacaatgg
      901 atgtcaattg tttcaattac ataagggatc ttgttcacag gacaaaacct gaccttatcc
      961 tcattacagg ggatttggta tatggtgagt ttgacgacct gagtacttcc tgggaaaagt
     1021 atgtgaagtt catggatggc tttaacattc cctgggctcc catattcgga aaccatgaca
     1081 atgagagcat aaagggagtt gactggcagt gtgaaataat agaaaaggcc agttactccc
     1141 tttttaaaag agggaatgta acaggcaaca gcaattacac aatcggaatt gtgcaaaaca
     1201 gcaaattcaa gagagtcatc tacatgatgg actccaatgg atgcggtaat acaagcgatc
     1261 caagtgttaa aaagacggtt ggttttgcgt tagatcagtt gacttggttt gatgaaacgg
     1321 ccaatagaat tgaaagcttt gcaggtaaag tgaagaaatt tgtctgctac cacataccaa
     1381 ctttggaatt tgctcttgca gcgacaaatg cagggtatca gaaggaactg tatgacaatg
     1441 cagcttatac gataggtgag gatgtgccag ctaaaaactc ggactttgga agaaaaggcg
     1501 agatttttaa aggactgcat gaagttccgg acttacttgc cttgttcaaa aagcattctg
     1561 ttgacggagt gtttgtaggg cacagccact gcaacagtat aagtgtattg tatgaaggta
     1621 tcaggtggac gttt
//
LOCUS       DAONVY010000508         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_49777_length_1627_cov_4.522901, whole genome shotgun sequence.
ACCESSION   DAONVY010000508 DAONVY010000000
VERSION     DAONVY010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49777_length_1627_cov_4.522901"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLI11_10755"
     CDS             complement(<1..730)
                     /locus_tag="PLI11_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HPZ53389.1"
                     /translation="MVPKRIASAIMNSLKGGVVPRVGLGYIAVGREKEIDALLGDIDV
                     VQNGGASFRFIVGRYGSGKSFLLQTIRTYAMDKGFVVVDADLSPERRFMGSQKQGLAT
                     YRELMKNMSTKTKPDGGALSLILEKWIYAIQQKVMREKELSAFDAKLSTEVELRIFET
                     VGELKELVHGFDFAKVLSLYWRAYRDGNEEMKSNVLRWFRGEYTTMTEARRDLGVNVI
                     ITDSDWYDYLKVFASFLVKAGYKGL"
     gene            complement(735..>1627)
                     /locus_tag="PLI11_10760"
     CDS             complement(735..>1627)
                     /locus_tag="PLI11_10760"
                     /inference="COORDINATES: protein motif:HMM:NF026952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tellurite resistance TerB C-terminal
                     domain-containing protein"
                     /protein_id="HPZ53390.1"
                     /translation="LIDLCMPKVLEKLDELFSKYDLSLPAYILGERQKLERKDGIFND
                     LQYVRLRKGKKTAVINDYLAYEWKDEICISRCGYCGLKESTYVLYGIIVKLAEIALRN
                     LLSFKGTLKSPLDITDKTSDEIRYLIETIGAEKLGDCVMAAAAEVLGEKGVFVDDAIP
                     TPKIEKVEIDFDKLDDIREVSQKLTEKLVINDEEEVEEVAVAEEKTNDICDIIDVSGE
                     NPYNSFVRMLNDIQMHLLKAIANKEDAYSVARKLGVMLEVVIEDINEVALETTGDTVV
                     DVDCTIIEDYLDDIKKVLGG"
BASE COUNT          458 a          428 c          220 g          521 t
ORIGIN      
        1 acaagccttt ataacctgct ttaacaagaa aggacgcaaa cacctttaag tagtcatacc
       61 agtcggaatc cgtaattatc acattcacac ccaaatccct tctggcttcc gtcatggttg
      121 tatactctcc tctgaaccat ctcaggacat tactcttcat ttcctcattt ccatccctgt
      181 acgcacgcca gtaaagactc aacacctttg caaaatcaaa tccatgtacc agttccttca
      241 attcgccaac cgtttcaaat attcttaact caacttcagt agatagtttt gcatcaaaag
      301 cggacagttc cttttccctc attactttct gctgtattgc atatatccat ttttcaagta
      361 tcaaggacaa ggctccgcca tcgggcttgg tttttgtgga catgttcttc ataagttccc
      421 tataggttgc caagccctgc ttctgtgaac ccataaacct tctttcggga gacaagtcgg
      481 catccactac cacaaaccct ttatccatgg catatgtcct aatggtctgt aagagaaagc
      541 tctttccact tccgtatcta ccaacaataa atctaaatga agcgccacca ttctgcacaa
      601 catcaatatc accaagcaag gcatctattt ccttctccct gcccaccgca atatatccaa
      661 gaccaactct tggaacaact ccacctttca gtgagttcat tattgcggaa gcaattcttt
      721 tcggaaccat atagttaacc tcccaaaact tttttaatgt catctaaata atcttcaatt
      781 atcgtgcaat ccacatccac tacggtatcg cctgttgttt caagagccac ctcatttatg
      841 tcctcgataa caacttccaa cattacacca agtttcctgg caacagaata tgcatcctct
      901 ttgttggcta ttgcctttaa caaatgcatc tgaatatcat tcagcattct tacaaacgaa
      961 ttatatggat tctctcctga aacatcaatt atatcacaaa tatcatttgt tttctcttcc
     1021 gctactgcaa cttcttcaac ttcttcctca tcattaataa caagcttttc agttagcttt
     1081 tgggatacct ctctaatatc atccagcttg tcaaaatcaa tttccacctt ttcaattttg
     1141 ggagtgggta tcgcatcatc aacgaatact cctttttctc ccaaaacttc tgcagcagct
     1201 gccataacgc agtcgccaag cttctccgcc cctatggtct caatcaaata ccttatctca
     1261 tcacttgttt tatccgtaat atccagagga gacttgagag ttcctttgaa ggacaacaga
     1321 tttctcaagg caatttctgc cagtttgact atgataccat atagaacata agtcgattct
     1381 tttaaaccac agtatccaca acggcttatg catatttcgt ccttccattc atatgccaaa
     1441 taatcattta taactgccgt cttctttcct tttcttaatc tgacgtattg caaatcatta
     1501 aaaatcccgt ctttacgttc aagcttttgt ctttcgccca atatatatgc aggcaaagaa
     1561 aggtcgtatt tagaaaagag ttcgtccagt ttttcaagca cctttggcat gcagagatct
     1621 ataagat
//
LOCUS       DAONVY010000509         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_49792_length_1627_cov_2.680025, whole genome shotgun sequence.
ACCESSION   DAONVY010000509 DAONVY010000000
VERSION     DAONVY010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49792_length_1627_cov_2.680025"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="PLI11_10765"
     CDS             complement(<1..1495)
                     /locus_tag="PLI11_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53391.1"
                     /translation="MEKTQNSKELLVFFKESRLVYILNLDNQKLTKLDLNLPEGVNGV
                     YPGKNPGEYIVTADEEIFSSVYLVKDNTNEKINVGWRENRFYSTFAQDNPDGYVDRGI
                     FAHIRTDLSSQIMGRLAELNSVEDALNNASFSEWRRSISSNQIEQYHTTYTMWEPCFT
                     HRWNSITQTSNMSKIIDDKTGLPKYMALGKDNLTTNYHELNSAFLNGSYADGLLPMSK
                     LRLYPLRTFLQDLSVEFRSNPERLVAVSPVHEHEINVAGSIGDYNYYMVLGFRSHLLN
                     LYGSVEKINERFGTNFASVDEIDPPRDENRGKWDRYGGSDYFAYWSLYNRFIVNKRIL
                     EAYREALLAGFPPESISAHQIPEGDAVAGFLGEANTRLSPVDVVMSCGTAFGGTRYGT
                     WYEQKHNWLINAYNAGHKNITIGEYSSLAHGDIAAYNQLKYLFNHGVRMTHVLVPYPS
                     DSSDYVIVKDKEMLAVYKLQRENNPRPGYTGGTLDVKHIFQDGKSYSV"
BASE COUNT          437 a          358 c          303 g          529 t
ORIGIN      
        1 ctacactgta ggattttcca tcctggaata tgtgctttac atccaaagtg ccaccagtat
       61 aaccaggtct tggattgttc tccctctgca acttgtatac ggcaagcatt tctttgtcct
      121 ttacaataac gtaatcagaa gaatcgcttg gatacggtac aagcacatgg gtcattctga
      181 caccatgatt gaaaagatat ttaagttgat tatatgctgc aatgtcaccg tgggcaagcg
      241 aactgtattc acctattgtt atgttcttgt gacctgcatt gtaagcgttt ataagccagt
      301 tgtgcttctg ttcataccag gtaccatatc tggttccacc aaaggcggtt ccgcaggaca
      361 taaccacatc aacaggagac aatcttgtgt tggcttcacc taagaatcca gcaacggcat
      421 ctccttccgg tatctgatga gcacttattg attccggtgg aaaaccagca agcagtgctt
      481 ccctgtatgc ttccagaatt cttttgttga caataaaacg gttgtatagg ctccagtaag
      541 caaaataatc gctgccacca tatctgtccc atttacccct gttttcatct ctaggtgggt
      601 ctatttcgtc tacggaagca aagtttgttc cgaatctttc atttatcttc tcaacacttc
      661 cgtacaggtt caaaagatgg cttctaaagc caagcaccat ataataatta tagtctccga
      721 ttgaacctgc tacatttatt tcatgttcat gcacaggcga cactgccacc agtctttcag
      781 ggttgcttct gaattcgaca gacagatcct gcaaaaaagt tcttaaagga tatagtctca
      841 atttgctcat tggaagaaga ccatctgcat aacttccatt aagaaatgca ctgttaagtt
      901 catgatagtt tgtagtcaaa ttgtccttgc caagggccat gtactttggt agccctgtct
      961 tgtcgtcaat aattttagac atgttggacg tttgagtaat cgagttccat ctatgagtaa
     1021 aacatggctc ccacatggtg tatgtggtat ggtactgctc tatttgatta ctgctgatgc
     1081 ttctcctcca ctccgagaaa gatgcattgt ttaatgcatc ttccacacta ttaagttcag
     1141 caagtctgcc cataatttga gaagacaaat ccgtccttat atgtgcaaag attccacggt
     1201 ctacataacc atcgggatta tcctgtgcaa aggtggagta aaacctattt tccctccatc
     1261 caacatttat cttttcattg gtgttgtctt tcacaaggta aacacttgaa aaaatttctt
     1321 cgtctgcggt aacaatgtac tctcctggat ttttgccagg atagactccg ttaactccct
     1381 ccggaaggtt aaggtctagt tttgtcaatt tctgattatc gagatttaat atgtacacca
     1441 gcctggattc cttgaagaac accaacaact ctttagaatt ctgagtcttt tccaagcgaa
     1501 tcctttcacc ctttggtaag gagctgtctt gcaaagtaat atgtttaaca tattttgcac
     1561 tatctatgtc gataatagct atttttactg aattatttgg atacatgctg gttatgagta
     1621 gttgctc
//
LOCUS       DAONVY010000510         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_50337_length_1615_cov_2.882051, whole genome shotgun sequence.
ACCESSION   DAONVY010000510 DAONVY010000000
VERSION     DAONVY010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50337_length_1615_cov_2.882051"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..1409
                     /gene="asnA"
                     /locus_tag="PLI11_10770"
     CDS             423..1409
                     /gene="asnA"
                     /locus_tag="PLI11_10770"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPZ53392.1"
                     /translation="MYKSVLDLRETQVAIKECKDYFERELAKALNLIRVTAPLFVESS
                     SGLNDELNGVERPVEFDLLETGESVQIVQSLAKWKRFALGRYKFKENEGIYTDMNAIR
                     RDEYVDEIHSVYVDQWDWEMVISKERRNLDTLKETVDKIYQVILNTQKHIKNKFPALE
                     ENELPDKIYYITTQELEDRYPTLTPKQREYEITKEKGAVCIMQIGIKLASGKPHDGRA
                     PDYDDWSMNCDILLYYKLLDIAMEISSMGIRVDEHSLVAQVKAANCEHRLEYPFHKAV
                     LNRELPYTIGGGIGQSRLCMFFLQKMHIGEVQVSVWPEEHERKFREKGCFLL"
BASE COUNT          553 a          317 c          352 g          393 t
ORIGIN      
        1 gcaataaatg agataaccaa gaaatggagt atgcactggg aataataatg aggcggttaa
       61 tgatttactt cgaagacaga ttggcagctg caaaagcggt ttgagaagca tatcggggat
      121 ctgccccgga acgtagcttt ttggccattt ttattatctt ccttctagcc ttcggagagc
      181 acgatttaca tccatcagaa cctgtctaac ataactgtaa gatacattta tgttcttcct
      241 tgtattcaaa attttgcaaa aatgaatgaa cttatccgct gattaatcca cacgggacat
      301 aaaatgcagt acgataagac attatcatat atcaaccaca atcaaccaca gaaacaaaac
      361 agcaatgttg taacattgcc acactgatga tataattgat agaaaaaaga aagaggaaat
      421 gtatgtataa atctgttttg gaccttagag aaactcaggt ggcaataaag gaatgcaaag
      481 actactttga aagagaactg gcaaaagcac ttaacttgat aagagtaacc gctccattgt
      541 ttgtagaatc ttcttcagga cttaatgacg aactaaacgg ggttgaaaga cccgtggaat
      601 ttgatttgct tgaaacagga gaatccgttc agatagtaca gtcccttgcc aaatggaaga
      661 gatttgcgct gggaagatac aagtttaagg aaaatgaagg catttacacc gacatgaatg
      721 caatcagaag ggatgaatac gtggacgaaa ttcattcagt ctatgtggac cagtgggact
      781 gggaaatggt catatccaag gaaagaagga acctggacac attgaaagaa accgtggaca
      841 agatctatca ggttatactg aacacgcaaa aacacataaa aaacaagttt cccgcccttg
      901 aagaaaatga attgcctgat aaaatatatt atatcaccac acaggagttg gaagacagat
      961 accctactct tactccaaaa caaagggagt atgagataac caaggagaag ggtgccgtat
     1021 gcattatgca aataggcatc aagcttgctt ccggcaaacc acatgacgga agagctcctg
     1081 actatgacga ttggtctatg aattgcgata ttcttctgta ctacaagctc cttgatattg
     1141 ccatggagat ttccagcatg ggaatcagag tggatgagca ctcccttgta gctcaggtca
     1201 aggctgcaaa ctgcgaacac agactggaat atccttttca caaggcagtg cttaatagag
     1261 aacttcccta taccataggc ggaggcatag gtcagtcaag gctttgcatg ttcttcctgc
     1321 agaagatgca tataggggag gtccaggtgt ccgtatggcc tgaagaacat gagagaaaat
     1381 tcagggagaa aggatgtttt ttgctttagc tcaataataa ttccaaggag gctgctgtca
     1441 tgaagacaca aagaaaagta ttgctcttaa tacttgttgc ccttttgttt ttcattgcag
     1501 gatgcagcaa ggaaaagcaa aacggcactg aaacaccaac cccgataccc actgaagcgg
     1561 aagatgcaac acctgattcc acacctgcac caacgcccac ctcaaccgat gataa
//
LOCUS       DAONVY010000511         1608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_50617_length_1608_cov_3.475209, whole genome shotgun sequence.
ACCESSION   DAONVY010000511 DAONVY010000000
VERSION     DAONVY010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50617_length_1608_cov_3.475209"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLI11_10775"
     CDS             complement(<1..1137)
                     /locus_tag="PLI11_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPZ53393.1"
                     /translation="MKDFKDIEYNPKSYWETMNEEEKKKVMEFSKDYIDFMNAGKTER
                     EVVEHIVDIVKNVGYEDFDEIIKQGKKLVPGQKIYSVSNEKALILAVIGESGPEKGFS
                     MIGSHVDAPRLDFKPLPLYEDSSFALAKTHYYGGIKKYQWVAIPLAMHGVVVKNDGTK
                     VRIRIGDKEDDPVFTISDLLPHLSQEQLKKEASNFIPGENLNVFLGSMPLKMDEDSKE
                     TNLIKKNILRYLYDNYGIREMDFATAEIEVVPAFKAKDVGLDLSLVGAYAQDDRICAY
                     TSLRAILEIEKSEKTCICYFSDKEEIGSVGITGARSNHFETFIYELCYALTNDSNPLI
                     ARRALKASKMLSADVTAGFDPTFPEVSDKTNATYLGKGVSIAKYT"
     gene            complement(1207..>1608)
                     /locus_tag="PLI11_10780"
     CDS             complement(1207..>1608)
                     /locus_tag="PLI11_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HPZ53394.1"
                     /translation="QLRHVKFLKDYEGIIEHMKKHARLLKPKFDIVLNSLEKELEGLD
                     ILEWSKPKGGYFISINTIDGLADKVVSLAKDCGVLLTPAGSTFPYKKDPRNRNIRIAP
                     SFPSVSDLAEAVRVLCVCVKICSIEYYYDKL"
BASE COUNT          422 a          349 c          284 g          553 t
ORIGIN      
        1 ggtgtatttt gctatactga cgcccttgcc cagataggtt gcattggttt tgtcggatac
       61 ctccgggaaa gttggatcaa atccggctgt aacatcagca gaaagcattt tgcttgcttt
      121 taatgctctt cttgcaatca atgggtttga atcatttgta agagcatagc atagttcata
      181 aataaatgtt tcaaaatgat tggatcttgc accggttatt ccaacactgc cgatttcctc
      241 cttgtcagag aaatagcaga tgcatgtctt ttcagatttt tcaatttcaa gtatggctct
      301 caatgaagtg tatgcgcaaa ttctgtcatc ctgtgcatat gctccaacaa ggctcaagtc
      361 tagacctacg tcttttgcct tgaatgctgg cacaacttca atctcagcgg tggcaaaatc
      421 catctcgcgg attccataat tgtcatacag gtatcttaat atgttctttt ttataaggtt
      481 ggtttccttt gagtcttcat ccattttcaa tggcatggaa cctaagaata cattcagatt
      541 ctctcctgga atgaaattgg aagcctcttt cttaagctgt tcctgcgaca agtgaggcag
      601 aaggtcgctt attgtgaaaa caggatcgtc ttccttgtcc cctattctta ttctgacttt
      661 cgttccatca tttttgacaa caaccccatg cattgcaaga ggaatggcaa cccattgata
      721 ttttttaata ccgccatagt agtgggtttt tgccagtgca aatgatgaat cttcgtacag
      781 tggcagcggt ttaaagtcaa gtcttggggc atccacatgg gatccaatca tgctgaagcc
      841 cttttctgga ccgctttccc caattaccgc caaaataagc gccttttcat ttgatacgga
      901 atatattttt tggccaggta ctagtttctt cccctgctta ataatttcat caaaatcttc
      961 ataaccgaca ttctttacaa tatcaacgat atgttcgact acttcccttt ccgttttacc
     1021 tgcattcata aaatcaatat agtcttttga gaactccatg actttctttt tttcttcttc
     1081 gttcatggtc tcccaatagc ttttgggatt gtattcaata tccttgaaat ctttcatagc
     1141 ttgacatttc ctttctataa aaaatgtaac atccaaatcg atgttacatt attataccac
     1201 aatttattag agtttatcgt aataatattc tattgagcag atttttacgc atacacacaa
     1261 caccctgacc gcttccgcca aatctgaaac agaaggaaat gaaggtgcaa tccttatgtt
     1321 tctgtttctc ggatcttttt tgtacgggaa ggtggaacct gcaggagtta acagtacgcc
     1381 gcaatctttg gcaagggaca caaccttgtc tgcaagaccg tcaattgtat taatgctaat
     1441 gaagtaacct ccttttggct tgctccactc aagtatgtca agaccttcca gttctttttc
     1501 caggctgttc aatacaatat cgaactttgg tttaagcaac cttgcgtgtt ttttcatgtg
     1561 ttcgataata ccttcatagt ctttaaggaa ttttacatgc ctaagctg
//
LOCUS       DAONVY010000512         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_50674_length_1607_cov_2.458763, whole genome shotgun sequence.
ACCESSION   DAONVY010000512 DAONVY010000000
VERSION     DAONVY010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50674_length_1607_cov_2.458763"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1607)
                     /locus_tag="PLI11_10785"
     CDS             complement(<1..>1607)
                     /locus_tag="PLI11_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53395.1"
                     /translation="TTSQLLGEKPYTVTMDVMSKEVNPAATCGAAFNVRAKKASTFMD
                     QGITFMVRNKSLRVFMKTRELAHIQLPFSFADEMRKVYIEDNLDVIRFHADDDNGKVL
                     LAEVELTEERVTVKDNKGKQKGNAKRENVPDTGFFGFMSHFAKTTVDNFSFEYYIEQY
                     EPADMSNFWDTYYDTWVATDDLERTLPVTYTNTVKKNKKVGIFYFLWHDRNGGPLFDH
                     YAAYLEGGIDKVWDIIKQGDEGYGHYWAEPYFGYYRSDDEWVIRKHTTMLVNAGIDFI
                     YFDMSNGHIYEHVLTKILGTWKQMREEGLKTPEFVCFLGDRTDLGYKTAMDVWNTVYQ
                     HGIYRDMYFMWDGKPLLLGNLAEVPDEIKENFTIRRSWAFTDWDWYTESDGKGKWPWI
                     ALHPQGPGKSFEGIIEQVIVSCGFHSNSSSGRSFHNGQQPTDGKNAFEFELETTPLGL
                     AFKEQWEHALKINPPIVMVTGWNEWWAGRWPNAGEGQKIANTYTITKDHPDYMHNYVD
                     CFNPEFSRDIEPMKYGFGDNYYYQMVS"
BASE COUNT          397 a          379 c          325 g          506 t
ORIGIN      
        1 agcttaccat ttgatagtag tagttgtctc caaaaccgta tttcatgggc tctatgtcac
       61 ggctgaattc aggattgaaa caatccacat aattgtgcat gtaatcaggg tggtccttgg
      121 ttatggtata agtatttgct attttctgtc cttctcctgc gtttggccac ctgcctgccc
      181 accattcgtt ccaacctgtt accattacaa taggaggatt tatcttaaga gcgtgctccc
      241 actgctcctt gaaggcaagt cctaaaggag tggtttccag ctcgaattca aaggcgtttt
      301 taccgtccgt tggctgctga ccattgtgaa agcttcttcc tgaggaggag ttggaatgga
      361 atccgcagga aactatcacc tgttcaataa ttccttcaaa gcttttgccc ggaccttgcg
      421 gatgaagtgc aatccatggc catttgcctt ttccgtcaga ttcggtatac cagtcccaat
      481 cggtaaacgc ccaggatctc ctaatggtaa agttctcctt aatctcatcg ggcacctctg
      541 caagatttcc aagtagcaaa ggttttccat cccacataaa atacatgtcc ctgtaaatac
      601 catgctggta taccgtattc catacatcca ttgctgtttt ataccctaga tcggttctgt
      661 ctcccaggaa gcagacgaac tcaggtgttt tcaagccttc ttctctcatt tgcttccatg
      721 taccaagaat tttggtaagc acatgctcat atatgtgtcc gtttgacatg tcgaaatata
      781 taaagtcgat gcctgcattt acaagcatgg ttgtgtgctt tctaataacc cactcgtcat
      841 cggaacggta gtagccaaaa tagggttctg cccaataatg gccataacct tcgtcgcctt
      901 gtttgataat atcccaaacc ttgtcaattc cgccttccag atatgcggca tagtggtcaa
      961 acaagggtcc gccgtttctg tcatgccaca gaaagtagaa gattcctacc tttttattct
     1021 ttttaaccgt atttgtatat gttactggaa gagttctttc caaatcatcc gtagcaaccc
     1081 atgtgtcata atacgtgtcc cagaagtttg acatgtcagc aggctcatac tgctctatat
     1141 aatactcaaa ggagaagttg tcaacagtgg ttttggcaaa gtgggacata aaaccaaaga
     1201 agcctgtatc gggtacattt tccctttttg cgttgccttt ttgcttgcct ttgttgtctt
     1261 taactgttac tctttcttct gtcagctcaa cttcagcaag caataccttg ccgttatcgt
     1321 catctgcatg gaatctaatt acatcgagat tatcctcgat atatactttt ctcatttcgt
     1381 ctgcaaagga aaagggcagc tgaatatggg caagttcacg ggttttcatg aacaccctca
     1441 aggatttgtt tcttaccatg aacgtaatgc cctgatccat gaaagtggat gcctttttag
     1501 ctctgacatt aaaagcagct ccgcaggttg ccgcagggtt gacctccttg gacatgacat
     1561 ccatggttac tgtatatggc ttttcgccga gcagctggga tgtggtg
//
LOCUS       DAONVY010000513         1599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_50988_length_1599_cov_3.456606, whole genome shotgun sequence.
ACCESSION   DAONVY010000513 DAONVY010000000
VERSION     DAONVY010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50988_length_1599_cov_3.456606"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLI11_10790"
     CDS             complement(<1..288)
                     /locus_tag="PLI11_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53396.1"
                     /translation="MTFDRKDKNGEEDRGYSRYIKKTVFAPYEISRKYFRVKFPYLLE
                     TEMTPVYVKGKEVTAGKTHVLVRNMGPGGFCFVSDLNLPVGKDIFYVFSSEI"
     gene            complement(382..1428)
                     /locus_tag="PLI11_10795"
     CDS             complement(382..1428)
                     /locus_tag="PLI11_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53397.1"
                     /translation="MNGENVYGRHYSVKEGKYDTRKNAKLYGVNFQEQWDYAIEIDPE
                     IIFITGWNEWVAGRFEEWGGVKNAFPDQFSDEYSRDIEPSKGDLKDHYYYQMVSNIRR
                     FKGTKPVPKASGKKTIDIYSDEDMWKDVSPNFFAYKGNTFDRDFNGYVDKKTNKPISY
                     ENRTGRNDLVSAKVARDNEFVYFMVECTENIVGEGDKSWMRLFIEIGGRQDANWESFH
                     YVVNRQKTSNGTAVLEKSTGGWNWEEVGLVEYSLKGKRLQIKVPKFMLGIKGDKFVIN
                     FKWSDNMQVDGDIMDFYINGDVAPNGRFKYQYATYSYEKSPLTRIATLVLASIVMATV
                     ILKNPFMNRRMPEK"
BASE COUNT          436 a          402 c          241 g          520 t
ORIGIN      
        1 aatctcactt gaaaatacgt aaaaaatgtc cttacccaca ggcagattca aatcagatac
       61 aaaacagaat ccaccaggcc ccatattcct caccaaaaca tgggttttgc cggcagtaac
      121 ctctttgcct ttgacataaa caggagtcat ctccgtttca aggagatatg ggaatttgac
      181 tctaaagtac tttctactta tttcatacgg agcaaacacc gttttcttta tgtaacggct
      241 gtatcctcta tcctcttctc catttttgtc ctttctgtca aaggtcatac ccaccaccct
      301 tattctgcag acatcttcta tcccaatatt acataacaat ggaaatattg tcaatctcat
      361 ataaagaaaa acgccagaga tttatttctc tggcattctt ctattcataa atggattttt
      421 taatatgact gtcgccataa caatcgaggc aagaactaaa gttgcaatcc tagttagagg
      481 actcttctca taagaatacg ttgcatactg gtatttgaat ctgccatttg gagcaacatc
      541 accatttatg taaaaatcca taatatctcc atcaacttgc atgttgtcgg accatttgaa
      601 gttaataacg aacttgtcgc ccttaatgcc cagcatgaat ttgggcacct ttatttgcag
      661 tcttttcccc ttcaacgaat actcaacaag cccaacttct tcccagttcc aacctcctgt
      721 ggatttttcc agcactgccg ttccatttga agttttctgc ctgtttacaa cataatgaaa
      781 gctctcccaa ttggcatcct gacgtccgcc aatttcaata aacagacgca tccaagactt
      841 gtcgccctct ccaactatat tctcagtaca ttccaccatg aagtatacaa actcattatc
      901 tcttgccacc ttggctgata ctaaatcatt tctgcctgtc ctgttttcat agctgatggg
      961 tttgtttgtc tttttatcca catatccatt aaaatccctg tcaaatgtgt ttcccttgta
     1021 tgcaaagaaa ttaggggata catccttcca catatcctca tcagaatata tgtcaatagt
     1081 ctttttaccg gatgcttttg gaacaggctt tgttcccttg aaccttctaa tattactaac
     1141 catctggtaa tagtagtggt ctttcaaatc tcctttggaa ggctcaatat ccctggaata
     1201 ctcatcactg aactggtcgg gaaaagcatt ctttactcct ccccattcct caaatcttcc
     1261 tgcaacccat tcattccacc cagttataaa tataatctcc ggatctattt caatggcata
     1321 atcccactgt tcctgaaaat tgacaccgta gagctttgca ttctttctcg tatcatactt
     1381 cccttccttg acggagtaat gcctgccata gacattctcg ccattcatgg cggtaagtcc
     1441 tctttttgca ttccagttct gggcaacaga tacggtgacc tgttcaggcg ttccatccgg
     1501 atttcttgcg attaactgag gatatatcga aatccagttc cagattatgt agttttctta
     1561 atctccggtt gaatcgctcc gtaaatcaaa ggatttccg
//
LOCUS       DAONVY010000514         1591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_51391_length_1591_cov_3.994792, whole genome shotgun sequence.
ACCESSION   DAONVY010000514 DAONVY010000000
VERSION     DAONVY010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51391_length_1591_cov_3.994792"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PLI11_10800"
     CDS             <1..1329
                     /locus_tag="PLI11_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53398.1"
                     /translation="IMDIIHFMFIFYKRQGDNYMSTKKSGEKSTKLVYEFVIVVLLLV
                     IIVLCVIIIQKRNNPDPIIKATPTPEPTGTISTEEPTQTPKPTVTPYELVEYKGRIEH
                     IFFHSLIAFPELAFTQTDKKALVTSTWFKDFVTVSEFKKILQALYNDGYMLINANDIF
                     EERTVDGVKRYEVKTFLFPKNKKPLIMSFDDTNYDSNKLGYGTVDKLILDDKGKVVTY
                     TRMKDGREIISDDNEYIPIIDKFVEEHPDFSFNGAKGMICLTGFDGILGYRTHRDSPN
                     KQSESEKVKPIVKALKDTGWYFASHSYKHGAMAGYNVAQMRDCAQKFKNEVTPLIGET
                     KIYVYPFGSWKTGEVQDILYKEFGFTYFCSVGVNYYIKTRADMNYILFQDRANIDGWT
                     LVRNGHLLEKYKDWGTPRFPSMIVDDIYNEEERGISRQEAMEIYSEMDNS"
BASE COUNT          580 a          246 c          355 g          410 t
ORIGIN      
        1 ataatggata taattcattt catgtttata ttctacaaaa ggcaaggtga taattatatg
       61 tccacgaaga aaagtggcga aaagtctacc aaattggttt atgagtttgt aattgtggtt
      121 ttgttgttag tcataattgt tttatgcgtg ataattatac aaaagagaaa caatccagat
      181 ccgataataa aagcaacacc aacaccggaa ccaactggaa cgataagtac ggaagaaccg
      241 acacagacgc caaagccaac cgtcacacct tacgagttgg ttgaatataa agggagaatt
      301 gaacatatat tttttcactc gctcatagcc tttccagagc tggctttcac ccagacagac
      361 aaaaaggcac tggtaacatc tacatggttc aaggactttg taactgtaag cgagtttaaa
      421 aagattcttc aagccttgta caacgatgga tacatgctga ttaacgccaa cgacatattt
      481 gaagagagaa cagtcgatgg tgtaaaaagg tatgaagtaa aaacatttct cttcccaaag
      541 aacaaaaagc ctttgataat gtcatttgac gacacaaact acgacagcaa caagttggga
      601 tacggtactg tggacaagct catattggat gacaagggca aggtggtaac gtataccagg
      661 atgaaggatg gtagggaaat aattagtgat gacaatgaat acatcccgat aattgacaag
      721 tttgtagaag aacatcctga tttttcattc aacggagcaa agggcatgat atgcctaaca
      781 ggttttgatg gaatactagg gtacaggact cacagggatt ccccaaacaa gcagtctgaa
      841 agtgagaagg taaaaccgat agtaaaggca cttaaggata ccggatggta cttcgcaagc
      901 cactcataca aacatggtgc aatggcaggc tacaatgttg cacagatgag ggactgcgcg
      961 cagaaattca agaacgaggt aacgcctttg ataggtgaga caaagatata tgtatatcct
     1021 tttggaagct ggaagacagg agaagtacag gatattttgt acaaagaatt tggatttact
     1081 tatttctgca gtgtgggagt caactattat atcaaaaccc gtgctgacat gaactatata
     1141 ctgtttcagg acagggcgaa catagatgga tggacattgg ttagaaacgg acacttgctg
     1201 gagaagtata aggattgggg tactcccagg tttccttcaa tgattgtaga tgatatttac
     1261 aatgaggagg aaagaggaat cagcagacag gaagctatgg aaatatatag tgaaatggat
     1321 aatagctgaa caagtggaga aactaatggt aattaggcag ataacacagc attagttgtg
     1381 caggttgcat aagcaaaacg aagttttttt gtaagattta ctactgttta aaatatgagc
     1441 caagtgatat aatagggaag tatgatatta cgtataaaat actattagtg aatacgttaa
     1501 aaaattacaa aaattacaat aaattcatgt gaacaataat aggaggttac ataatggcaa
     1561 gcgttgtttt taaaaacatc tacaagagat a
//
LOCUS       DAONVY010000515         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_51860_length_1581_cov_3.149410, whole genome shotgun sequence.
ACCESSION   DAONVY010000515 DAONVY010000000
VERSION     DAONVY010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51860_length_1581_cov_3.149410"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="PLI11_10805"
     CDS             <1..112
                     /locus_tag="PLI11_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HPZ53399.1"
                     /translation="GEQKFYKEKGFDNEPQRCADCRRARKTQRRPENRFR"
     gene            complement(148..855)
                     /locus_tag="PLI11_10810"
     CDS             complement(148..855)
                     /locus_tag="PLI11_10810"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPZ53400.1"
                     /translation="MANRLDKILSTMGFGSRKQVKELVKNGCVTVDGEVVKDSSMHID
                     TDIQELHVNGEKIEYKEFVYIMMNKPRGVISATEDEFGHETVIDLLDDGYKGYNLSCA
                     GRLDIDTEGLMIITNDGQLVHRIISPKKQVVKKYYCVLDYIITDKDINEFEKGIVLAD
                     AYKCLPARLEKISDKEAFVYITEGKFHQVKRMFYARRNNVRYLKRVQIGNLELDSKLE
                     PGEYRELLAEEIKMIFE"
     gene            943..1017
                     /locus_tag="PLI11_10815"
     tRNA            943..1017
                     /locus_tag="PLI11_10815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:976..978,aa:Glu,seq:ctc)
     gene            complement(1122..>1581)
                     /locus_tag="PLI11_10820"
     CDS             complement(1122..>1581)
                     /locus_tag="PLI11_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPZ53401.1"
                     /translation="KEIYDYLVKILNKEAYDGSGLIYGIGHAVYTKSDPRAVLLKKKA
                     EELAYEKGHEKEFELYRKVEELSLQAFEDVKKNGKDMCANVDFYSGFVYSMLNIPKEL
                     YTPIFAISRIVGWSAHRIEEMVAGKKIIRPAYKNVGAQKYVGKYIPLKER"
BASE COUNT          438 a          367 c          232 g          544 t
ORIGIN      
        1 tggcgaacag aagttctaca aagaaaaagg ctttgataat gaacctcaga gatgtgcaga
       61 ctgcagaaga gcaaggaaaa cccaaagaag accagaaaat cgtttcaggt aagacaaatt
      121 gaaagcagaa taatctgctt tcttttttta ctcaaatatc atttttatct cttccgccaa
      181 aagttccctg tattctcctg gttcgagctt gctatccagc tccaaattac caatctgaac
      241 tctttttaaa tatcggacat tgtttctcct tgcataaaac attcttttta cctgatggaa
      301 cttcccttcc gtaatatata caaaagcttc cttgtcgcta attttctcaa gtctggcagg
      361 caggcatttg taagcatctg ccagcactat tcctttttca aactcattta tatccttgtc
      421 ggttattata taatccaaaa cacagtaata tttttttact acttgctttt tgggagaaat
      481 aattctgtgt acaagctgcc cgtcatttgt aattatcata agtccctcgg tatcaatgtc
      541 caatctgcca gcacaggata agttatagcc cttgtatcca tcatcaagca aatctataac
      601 cgtttcatga ccgaactcgt cctcggttgc agagattact ccccttggct tgttcatcat
      661 gatatacacg aattccttgt actctatttt ttctccattg acatgcaact cctgaatatc
      721 cgtatcaatg tgcatggagc tgtcttttac tacctcacca tccacagtaa cacagccatt
      781 cttaacaagt tcctttactt gttttcttga accaaatccc attgtagaca atattttgtc
      841 caatctgttt gccatattat tccttctctc aaattcttaa tatttttttg atttacccta
      901 ttgattttta tataaagaca tagtatacta taacagctgt gaggctcatt ggtcaagcgg
      961 ctaagacacc ggcctctcac gccggagaca ggggttcgat tcccctatga gtcaccaaaa
     1021 aaggtcgatt gcttttagca gtcgactttt tttattctta cactaccaga attctgttgc
     1081 ccaataatat agcctaagac ttttcatctt aggctttaag tttacctttc tttcagagga
     1141 atatactttc ctacatattt ttgtgcacct acatttttat acgccggacg aataatcttc
     1201 tttccagcaa ccatttcctc aattctgtga gcactccaac caacaattct tgaaattgca
     1261 aagattggcg tgtacagttc ctttggaata ttcagcatgc tgtatacaaa tccggaatag
     1321 aagtccacgt ttgcacacat atcttttcca ttcttcttaa catcttcaaa agcttgaagc
     1381 gaaagctctt caacttttct atagagctca aactcctttt catgtccttt ttcgtatgcc
     1441 agttcttctg ctttcttctt caaaagaacc gctcttggat ccgattttgt gtaaactgca
     1501 tgtcctattc catatatcaa acctgaaccg tcataggctt ccttgttcag aatcttgaca
     1561 agataatcat agatttcctt g
//
LOCUS       DAONVY010000516         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_51914_length_1580_cov_2.782295, whole genome shotgun sequence.
ACCESSION   DAONVY010000516 DAONVY010000000
VERSION     DAONVY010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51914_length_1580_cov_2.782295"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLI11_10825"
     CDS             <1..559
                     /locus_tag="PLI11_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53402.1"
                     /translation="PQSLTDVINGNEFTVEIVLDNFTAKGTDFNTIMNCDNDNFSLFR
                     RVANNVMEFKNNTNARPVSKAGTGLDYLTSRVTLTITYKVGGKSTMYINGELIDSKDA
                     VNAIGANKMFFGHDNSTRNFSADYEYIRFYNRELSAEEVSKNYSEDLKAANPTTGVAA
                     SFFYMAITFGLAGAAYKGMKKRARI"
     gene            710..1138
                     /locus_tag="PLI11_10830"
     CDS             710..1138
                     /locus_tag="PLI11_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53403.1"
                     /translation="MKVAKDKYNIYFYVKTVKDITPPTDGSWMILFISTGETPSWYSY
                     NYVVNRVSPVDSEAVIEKVTDIWNFEKIGRAKLKVEGNQMMLAIPRNLIGFPVDKYSG
                     LINLQFKWADNYQPGDIRSFYTMDDAAPLGRLNFIFSNIA"
BASE COUNT          496 a          274 c          341 g          469 t
ORIGIN      
        1 gccgcagtct cttacagacg taattaatgg aaatgagttt acagtcgaga ttgtactgga
       61 caattttact gccaaaggta cggacttcaa cacaatcatg aactgcgaca atgacaattt
      121 ctcattgttc cgcagagttg ctaacaatgt aatggagttc aagaacaaca caaatgcaag
      181 gccggtatcg aaagccggaa cagggcttga ctatctgact tccagagtga ctttgacaat
      241 cacatacaag gtgggcggca agtcaaccat gtacatcaat ggcgagttga tagattccaa
      301 ggatgcagta aatgcaattg gcgccaataa aatgtttttt ggacatgaca attccacaag
      361 aaatttcagt gctgattatg agtacataag attctacaac agggaattgt cagctgaaga
      421 ggtttcaaag aattatagcg aagacttgaa ggctgcaaat cctaccactg gtgttgctgc
      481 atcattcttc tacatggcaa taacgtttgg ccttgctggc gctgcataca agggtatgaa
      541 gaagagagca aggatatgag aatatcaaaa tctttatagc tcgaaaggga gtctgcaaaa
      601 cgcaggctct ttttttgtac ctctttttta tacattttta agtctgatat ggtaatccgt
      661 tgacagtata tcttaggtag aatatatacg aatagtaaat gtactattta tgaaagttgc
      721 aaaagacaag tataatatat atttctatgt taaaacagta aaggatatta ctccgcccac
      781 tgacggtagt tggatgatct tgttcatatc aacaggtgaa acaccctcct ggtattctta
      841 caattatgtt gtaaacagag tgtctccggt tgattccgag gctgtaattg agaaggttac
      901 agacatctgg aactttgaaa aaataggacg tgcaaagctg aaagtggaag gcaaccagat
      961 gatgcttgcg attcctcgca atttgatagg ttttcctgta gataagtaca gtggtcttat
     1021 caatttacag ttcaagtggg cggataatta tcaaccaggc gatattcgga gtttctacac
     1081 catggatgat gctgcgcctt tgggcaggct aaactttatc tttagtaata tcgcttgata
     1141 tcttgcaggg gtgaccgccg caaagcgtcc tcctatatgg ttgcatgtgc agatgttcag
     1201 tgcataacga aaaccattta cacggcatag ttccatcctt tacatagata tcacaaatta
     1261 gcaaagggcc acagatattg cggctctttg ctgtttggat acatgtctat tttatatttc
     1321 tttcagtctg ttttctacaa catattgcct ttttgttaat atatacttat catgagcgta
     1381 gaatttataa agctataaag gatgaaggat aatgaaaaga aaacttctac tgttactatt
     1441 aaatttatgg gtgtgggtga ttatgatatc aatgggaaca aatggaagtc aattaaacgc
     1501 aaacaggaac ggagaaatgt cctgggtcaa gattccttat accgcaaagg aagatgttgt
     1561 tggtgactat ttatacgttg
//
LOCUS       DAONVY010000517         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_52015_length_1578_cov_2.883782, whole genome shotgun sequence.
ACCESSION   DAONVY010000517 DAONVY010000000
VERSION     DAONVY010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52015_length_1578_cov_2.883782"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLI11_10835"
     CDS             complement(<1..1443)
                     /locus_tag="PLI11_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPZ53404.1"
                     /translation="MSVDKVIECISCGRTSLGIEIGSTRIKSVLITDDHEPVAAGSFE
                     WESEYIDGNWTYSLDDVFKGIQESYRNLFEDVKKRYGLTLSRVGSIGISAMMHGYLAF
                     EGEKLLVPFRTWRNTYTEKASKELTGMFQYNIPQRWSIAHLYQAILNGEDHVKRIDYL
                     VTLSGYVHWQLTGKKVLGIGDASGMFPIDIHAKDYNGDMLCLFDSVLKDRNMPFRTRD
                     ILPKVLAAGADAGVLTEKGARLIDPAGRLKPGIPLCPPEGDAGTGMVATNSIARYTGN
                     VSAGTSVFAMFVLDKELKKVYPEIDLVTTPAGDLVAMVHCNNCSSELNAWVSLFREYT
                     LRMGFEVETGRIYETLFNMALEGEEDGGGLLAFNYLSGEHITGFEKGCPLFAYRPGNV
                     FNLANFMRTLMYSSVATLRIGMDILDQENIKINYLRGHGGFFKTKGVGERVMAAALNV
                     PVSVLDTAGEGGVWGIALLASYMKNRQKDET"
BASE COUNT          436 a          405 c          272 g          465 t
ORIGIN      
        1 agtctcatct ttttgcctgt tcttcatgta agaagcaaga agagcaatac cccagacccc
       61 tccttcaccg gcggtatcaa gcaccgacac aggtacattc agtgcagcag ccattaccct
      121 ttcaccaaca cccttggttt tgaagaaacc tccatgaccc ctcaagtagt ttatcttgat
      181 attctcctga tccaaaatat ccatacctat tctcaatgtg gcaacggatg aatacatgag
      241 tgttctcata aagttggcaa gattgaacac attgcctggt ctgtatgcaa aaagcgggca
      301 tcctttttca aaaccagtaa tatgttctcc ggaaagatag ttgaatgcaa gaagaccacc
      361 gccgtcttct tctccctcca aagccatgtt gaacaaagtt tcatagattc ttcctgtctc
      421 cacctcaaaa cccattctta gcgtatattc cctaaacaag gatacccagg cgttaagctc
      481 ggagctgcag ttgttgcagt gtaccatggc aaccaaatca cctgcagggg ttgtaaccaa
      541 atcgatttcc gggtatacct ttttaagttc cttgtccagt acaaacatgg caaatacact
      601 tgttcctgct gatacattac ctgtgtacct ggctatgctg tttgtggcaa ccatgcctgt
      661 tccagcatct ccttctggcg gacacaaggg tatgcctggc ttcagccttc ctgcagggtc
      721 aatcagtctt gctcccttct ctgttagaac acctgcatca gcacctgctg caagaacctt
      781 tggcagtatg tctcttgttc taaaaggcat gtttctatcc tttagtacgc tgtcaaatag
      841 acacagcatg tctccattgt agtctttggc atgaatgtca atcgggaaca tacctgaggc
      901 atccccaatc ccaagcacct tctttccagt cagctgccaa tgtacatatc cgcttagagt
      961 aacaagatag tcaatccttt taacatggtc ttccccattc aatatggctt ggtaaagatg
     1021 ggcaatgctc cacctttgcg ggatattgta ttgaaacatc cctgtaagct ccttcgatgc
     1081 tttttcagta tatgtatttc tccaggttct gaaaggtaca aggagctttt caccctcaaa
     1141 ggcaagatat ccatgcatca ttgcacttat tccaatggag cctactctgc ttagtgttag
     1201 tccatatctc ttcttaacat cctcaaaaag atttctgtag ctttcctgga tacctttaaa
     1261 gacatcatca agggaatagg tccaattgcc atcaatatat tcgctttccc actcaaaact
     1321 gcctgctgcc acaggttcat gatcatcagt aatgagaacg gatttgattc ttgttgaacc
     1381 aatttcaatt cccaaggacg ttctaccaca acttatgcat tcaataacct tgtcaacgct
     1441 catataccaa aacctctctt catttccaat gttatgtcaa aaatacaaca ataacatggg
     1501 agtgtcaatc gatgcattta ataataacaa aaaaccgcac atcatttaga tgcacggctc
     1561 ataattatct aaatcgtc
//
LOCUS       DAONVY010000518         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_52101_length_1576_cov_3.481920, whole genome shotgun sequence.
ACCESSION   DAONVY010000518 DAONVY010000000
VERSION     DAONVY010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52101_length_1576_cov_3.481920"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1073)
                     /locus_tag="PLI11_10840"
     CDS             complement(24..1073)
                     /locus_tag="PLI11_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPZ53405.1"
                     /translation="MSNTDSMLDLFLYETNELIDGLEEILLKTENTGFSEEDINEVFR
                     SMHTIKGSSGMMGLDSISNLTHAAEDFFAFIRDNPNVEIDGGEVTDLILRVVDFIKHE
                     LNAMRNEDYEQGDARYLVEEIKEYTESVKEGKKQKTFKVHIYFDEGAQMENIRAFGVL
                     NTIENMADIVSTEPMDLDDEKSTEEIQANGFIFIIKSDADENKIKSILQDTMFLKSLQ
                     VTEVEKTSRKRKVNIDTTIISDSARNFVSVEVGKLDTLLDIVGELVIAESMLGRTDLG
                     DEYEKAYNNLQRMTDELQNIVMSIRMVPVSGIFHKMQRVVRDLARKIGKDIDLVIKGE
                     QTEVDKKIIDNLSDL"
     gene            complement(1090..1443)
                     /locus_tag="PLI11_10845"
     CDS             complement(1090..1443)
                     /locus_tag="PLI11_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPZ53406.1"
                     /translation="MKTVLIVDDAAFMRLSIRKMLEKNGYEVVGEAENGLVAVEKYME
                     LQPDIVTMDITMKDMNGLEALKAIMERDKNANIIMVSAMGQEAMVKDAVLSGAKGFIV
                     KPFKEETLIKALEKF"
BASE COUNT          404 a          389 c          231 g          552 t
ORIGIN      
        1 gactgcattt cttattatat gtatcaaagg tcggacaaat tgtcgataat cttcttatca
       61 acctccgtct gctccccctt tatcaccaga tcaatatctt tgccaatctt tcttgcaagg
      121 tctctgacca ctctctgcat cttgtggaat atgccggata cgggcaccat cctaatactc
      181 atgacaatat tctgaagctc gtccgtcatt ctctgcagat tgttgtatgc tttctcatac
      241 tcatcaccaa gatcggtcct tccaagcata gattcggcta taaccaattc ccccactatg
      301 tccagcaagg tatccagctt accaacttcc acactgacaa agtttctcgc actatcacta
      361 attattgtcg tgtcaatatt aacttttctc tttcttgatg ttttttcaac ttcggtaacc
      421 tggagcgact tcaggaacat tgtatcctgc aaaatgctct taatcttgtt ttcatccgca
      481 tcagatttga ttataaagat aaagccgttt gcctggattt cttccgtact tttttcatca
      541 tccagatcca taggctccgt acttacaatg tctgccatat tttcaatggt attcaatacg
      601 ccaaatgcgc gtatgttttc catctgagcg ccctcgtcaa aatatatgtg aaccttgaag
      661 gtcttctgtt ttttaccttc cttcacactc tcggtatatt cctttatctc ctcaacaagg
      721 tagcgggcat ctccctgctc ataatcttca tttcgcattg cattcaattc gtgttttata
      781 aaatcaacaa cacggaggat gaggtcggtc acttctcctc cgtcaatttc cacattcgga
      841 ttatccctga taaatgcaaa gaagtcttcg gctgcatgcg taagattgga tattgaatca
      901 agacccatca tgcctgatga gccttttatt gtatgcatgc tgcggaacac ttcgtttatg
      961 tcttcctctg aaaatccagt attttctgtt tttaaaagaa tttcttccag tccgtcaata
     1021 agttcatttg tttcgtaaag aaacaaatcc aacatggaat ccgtattgct caatatgttt
     1081 caccacactt tagaatttct ccagggcctt gataagggtc tcttccttga atggctttac
     1141 aatgaatcct ttggctcctg atagaaccgc atctttgacc atggcctcct gtcccattgc
     1201 tgataccatt atgatatttg catttttatc tctttccatt attgccttta acgcctcaag
     1261 accgttcatg tctttcatgg taatatccat tgttacaata tcaggttgca attccatata
     1321 cttttctacg gctaccaagc cgttttctgc ttctcctaca acttcataac catttttctc
     1381 aagcattttt ctaatagata gtctcataaa tgcagcatca tccactatca aaacagtttt
     1441 cattccatac ctccgataaa ccttgaattt cggttacata tatgtaatcg ataagttaag
     1501 aaaaatattt aatcaaattt tcctggattc cctattcaca taacggactt ccacttctcc
     1561 ggtatccgta tgtaat
//
LOCUS       DAONVY010000519         1556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_52997_length_1556_cov_3.279147, whole genome shotgun sequence.
ACCESSION   DAONVY010000519 DAONVY010000000
VERSION     DAONVY010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52997_length_1556_cov_3.279147"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PLI11_10850"
     CDS             complement(<1..579)
                     /locus_tag="PLI11_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53407.1"
                     /translation="MNKRVFYIVFILLFVLILSLTACQSGGEKDAPTPTPTEEAERTP
                     TPTPPPTATPAPTPRPLSEEQKGIPQEESEEIFKDEFKDDKISIDAFTRGGQADFYAV
                     IDEQLYLSYDGYEFVDAWDTYAPDWYLTVGDTHSQYEIYIDMQTTFERDDQWMACFLG
                     VRTQYNSGEHGIPTDPSSGFWVAICKDRATIYP"
     gene            731..1444
                     /locus_tag="PLI11_10855"
     CDS             731..1444
                     /locus_tag="PLI11_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53408.1"
                     /translation="MHAFLKNYIDSNKNNHEVKIGPYYVDILNEEGVYEIQTKQLFRL
                     RKKLHVLLPEYVVTIVLPLIKTKTIYWIDTTSNEVSKGRKSPKKCTWYDVFSELYAIK
                     DLLDNSNMKIMPILLDVDEYRLLNGWSYDKKKGSTCHDRIVTNVHESMMIASKEDYCK
                     LVPDSLAEDFSSKDFAKEAHTSIKNARLTLNILRHLGVVKQISKKGNLLIYNRSIQKR
                     RKDIPSTPNSHMYKHTTGY"
     gene            complement(1441..>1556)
                     /locus_tag="PLI11_10860"
     CDS             complement(1441..>1556)
                     /locus_tag="PLI11_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPZ53409.1"
                     /translation="VGKEFNVTRERIRQIEAKALRKLRHPSRSKKLRDYID"
BASE COUNT          510 a          320 c          279 g          447 t
ORIGIN      
        1 tggataaata gtagctctgt ccttgcaaat tgcaacccag aatcctgaag aggggtctgt
       61 tggaataccg tgttcgccac tgttatactg tgttctaaca cccaggaaac aggccatcca
      121 ctgatcatct ctttcaaaag tagtctgcat atcaatataa atctcatact gactgtgggt
      181 atctccaacc gtcaaatacc aatcaggagc atatgtatcc catgcatcaa caaactcata
      241 accgtcatag ctcaaataca actgttcgtc aataactgca taaaagtctg cctgaccacc
      301 tcttgtaaac gcatcaatgc tgattttgtc gtccttgaac tcatctttaa aaatctcctc
      361 agactcttcc tgtggaatac ctttttgctc ttcagatagc ggtcttggcg ttggtgctgg
      421 tgttgccgtt ggcggaggag taggtgttgg cgtcctttcc gcttcctctg taggagttgg
      481 agtcggagcg tccttttcac cgcccgactg acacgctgtc aatgacaata ttagaacaaa
      541 cagtaaaata aaaacgatgt agaatactct cttgttcata aataccatct ctctttctat
      601 atttgttaat taaaaatata atattaaatt aacaaattgt caatgtttta attgtatgtt
      661 agactaaaag caaaaagggg taattaacat tcaggatata aatacaatcg gtacactggg
      721 cgaaaaaagt atgcacgcat ttctaaagaa ctacatagac agcaacaaaa acaatcatga
      781 agttaaaata gggccatact atgttgacat attgaacgaa gaaggagttt acgaaatcca
      841 gacaaaacaa ttattcaggc tcagaaaaaa actacatgtc cttttacctg agtatgttgt
      901 cacaattgtt ctgcctttga ttaagacaaa aacaatatac tggattgata ccaccagcaa
      961 tgaagtttca aaaggaagaa aatcacctaa gaaatgcaca tggtacgatg ttttttcaga
     1021 gctttatgct attaaagatc tattggataa ttcaaatatg aaaatcatgc ctatcctcct
     1081 tgacgtggat gagtatagac tcttgaacgg atggagctat gacaaaaaga aaggttcaac
     1141 atgccatgac cgtattgtga caaacgtaca cgaaagcatg atgatcgctt ccaaagaaga
     1201 ctattgtaaa ctggtaccag attccttggc agaagatttt tcttcaaagg attttgcaaa
     1261 agaggcacat acgagtatca agaatgcaag attgaccttg aatattcttc gacatctcgg
     1321 ggtagtcaaa cagatatcaa aaaaaggcaa cctgttaatc tacaacaggt ccatacaaaa
     1381 aaggcgaaag gatattcctt ccaccccaaa cagtcatatg tataaacata caaccggtta
     1441 ttaatctata tagtctctaa gtttcttgct gcggcttgga tgtctgagct ttctcaaagc
     1501 ctttgcctca atctgtctta ttctctctct ggtcacgtta aactccttgc ccactt
//
LOCUS       DAONVY010000520         1545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_53501_length_1545_cov_3.712752, whole genome shotgun sequence.
ACCESSION   DAONVY010000520 DAONVY010000000
VERSION     DAONVY010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53501_length_1545_cov_3.712752"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLI11_10865"
     CDS             <1..1054
                     /locus_tag="PLI11_10865"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPZ53410.1"
                     /translation="NVDSDAKFIDLMKAAGEDNTAKLVAKYFNFIREKAVYMNPKDAG
                     MLLSNDYGNARVLKKTYDNLAKLRDMEELVIFPGFFGYSEDGEVVTFSRGGSDVTGSV
                     IAAAVNATVYENFTDVDYVFCANPKVIKDPKPISYFTYEEMRELSYAGFNVLHEETLE
                     PVYRKKIAVNIRNTNNPQSPGTMIVPAERAERVSGENPVVGIAADKGFITVHVEKYMM
                     NREIGFGRRLLTIFEKEGVSYEHAPSGIDSISVIVKERNCPKDKLERITDAIRKELEV
                     DDVVVEHGLAIIMLVGRGMSRTVGIASRATSAIARANVNIRMINQGSSEVSIMFGVHE
                     DDVEKSLIALYDEFFR"
     gene            complement(1083..>1545)
                     /locus_tag="PLI11_10870"
     CDS             complement(1083..>1545)
                     /locus_tag="PLI11_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPZ53411.1"
                     /translation="TFAMGPIAGVTIQFIKDLVHLLISNQSMGIGLLADFAIGSAYIL
                     TAGLIYKRLHTKKGALLGCTAGTVAATIVGAIFNYYVLIPLYVLLTPYSLEDIISMGT
                     KVTGSIKDLKTLIMFATVPFNLLKFGVISLLTMLTYKKVSKFFKMIRKESR"
BASE COUNT          492 a          263 c          363 g          427 t
ORIGIN      
        1 aaatgtggat tcagatgcca aatttataga tcttatgaag gcggcaggag aggacaatac
       61 tgctaaactg gttgcaaaat acttcaactt tattcgtgaa aaggccgtat acatgaatcc
      121 taaagatgcg ggcatgcttt tgagcaacga ttatggaaat gccagagtac ttaaaaaaac
      181 gtatgacaat cttgcaaagc taagagacat ggaggagctt gtcattttcc ccggattttt
      241 tggatactct gaggacggag aagtggtaac cttctcaaga ggaggatcgg atgtaaccgg
      301 ttcagtaata gctgcggccg tcaatgccac cgtgtatgag aactttaccg atgtggatta
      361 tgtattctgt gcaaacccaa aggttataaa ggatccgaaa cccatttcat attttactta
      421 tgaagagatg cgtgagcttt cctatgcagg tttcaatgtt ctgcatgagg aaacccttga
      481 accggtttac agaaagaaaa tagcggttaa cataaggaat acaaacaatc ctcaatcacc
      541 aggaaccatg atagttccag ctgaaagagc ggaaagggtt tcgggagaga atcccgtagt
      601 tggaattgct gcagacaagg gatttattac tgtgcatgtt gaaaaataca tgatgaacag
      661 ggaaattggt tttggtagaa gattgcttac aatctttgag aaagagggag tttcctacga
      721 gcatgcacct tccggaattg acagtatttc tgttattgta aaagagagga attgtccaaa
      781 agacaaactt gaaagaataa ccgatgccat caggaaagag cttgaggttg atgacgtggt
      841 ggttgagcac ggccttgcga ttataatgtt ggttggaaga ggaatgtcaa gaactgttgg
      901 tattgccagc agggcgacat cagccattgc aagggcgaat gtgaacatca gaatgataaa
      961 ccagggctcg agtgaagtga gcataatgtt cggagtacat gaggacgatg tggaaaaatc
     1021 attgattgcc ctatatgatg agttctttag ataaaaaaat acagcttgaa agctgtattt
     1081 ttttatctgg attcttttcg aatcattttg aaaaacttcg atactttttt gtaagtaagc
     1141 atcgttaaca atgatattac accaaatttc aagaggttga acggtacagt cgcaaacatt
     1201 atcagagttt ttagatcttt tatgcttcca gttacctttg ttcccatgct gataatgtct
     1261 tccagagagt agggtgtcaa taacacatat agaggtatca acacataata gttgaaaatt
     1321 gcgcctacta ttgttgctgc aactgttcct gctgtgcatc cgagaagagc gcctttcttt
     1381 gtatgaaggc gtttgtaaat taggccagca gtcaatatgt atgcagagcc aatagcaaag
     1441 tcagccaaaa gaccaatacc catggattgg ttggaaatca gcagatgaac aagatccttt
     1501 ataaattgaa tggtaacacc tgctatgggt cccattgcaa aggtc
//
LOCUS       DAONVY010000521         1545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_53503_length_1545_cov_3.161074, whole genome shotgun sequence.
ACCESSION   DAONVY010000521 DAONVY010000000
VERSION     DAONVY010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53503_length_1545_cov_3.161074"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLI11_10875"
     CDS             complement(<1..396)
                     /locus_tag="PLI11_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPZ53412.1"
                     /translation="MGKVLGNTQAVKQAHLDYLETFIDSVQSPESFVPIELVNIMAYI
                     TGETGKEIGIYINRRNRVEAIVIGDDRSVSLPFMDNRRGSNRLSGIRFLHTHPGGSSL
                     PSDVDLNSLKTLRLDGMVVVGVVNYNDKPH"
     gene            complement(455..>1545)
                     /locus_tag="PLI11_10880"
     CDS             complement(455..>1545)
                     /locus_tag="PLI11_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPZ53413.1"
                     /translation="YFYGSMHVTPTAISQVVEQIVRIGVIFVLAVKIAGQDLAYACAL
                     ATVSAAIGEMANLLVVGCVFYKETKKPYLKSATLSRRKTAIVIAKRSIPVSINRLLTS
                     IMGAVEAILLPARFLAGGLDYKSSIEALGRLSGMASPLIYFPTVITSALATQLVPAIS
                     QALSLKRHKLANLRISRCIKLSFLLGFACFSLFYTFGDYIGDFLYKGQDVGSILKNMS
                     WLCILLYLQQTMMGILNGLGKQGYALFSTTVGYIVRISFVWFVVPIYGVNGYIVGSFA
                     SMLIMTTIDILYLVYQTRMFLDLREWLLKPMLPAIVIFSAGTFFLEYLKKYHYGWMLA
                     GGLSCICGFLVASLFYLNKFPQRPIQKI"
BASE COUNT          475 a          348 c          291 g          431 t
ORIGIN      
        1 atggggcttg tcattgtagt taacaacacc aacgacaacc ataccgtcga gtcttaaagt
       61 ttttaaagaa ttcaaatcca catctgatgg cagacttgaa ccaccagggt gagtgtgcaa
      121 aaaccttatg ccggacaacc tgtttgaacc ccttctgtta tccatgaaag gaagggaaac
      181 tgacctgtcg tctccaatta caatggcttc aaccctgttt cttctattaa tatatatacc
      241 aatttccttg ccagtctctc ccgtaatgta agccattata ttaacaagtt caatcggcac
      301 aaaactttca ggcgattgca ctgaatctat aaatgtttca agataatcca gatgagcttg
      361 ttttacagct tgtgtgtttc caagaacttt tcccatatac cctccaaaac atgtacttat
      421 tctattgtac cccaaattta ataagtttgg caaatcatat cttttgtatt ggacgttggg
      481 gaaatttgtt aagatagaaa agcgatgcaa ccagaaagcc gcaaatgcat gacagtcctc
      541 cagccaacat ccatccataa tgatattttt ttaaatactc taaaaagaag gtgccagccg
      601 agaatatgac aattgcaggc agcatgggtt taagcagcca ctctctcaga tcaagaaaca
      661 tccgggtttg ataaacgaga tatagaatat ctatggttgt cattatcagc atgcttgcaa
      721 aggatccgac aatatatccg ttaacaccat atatgggcac tacaaaccat acaaaactaa
      781 tcctgactat atatcccact gttgtgctga aaagagcata tccctgtttc ccaaggccgt
      841 tcagaattcc catcattgtc tgctgaagat aaaggagtat gcatagccag gacatgtttt
      901 tcaatatgct tcccacatcc tgacctttgt ataaaaaatc tccaatataa tctccgaagg
      961 tgtagaacag ggaaaaacat gcaaatccaa ggaggaaact taattttatg cacctggata
     1021 tcctgagatt tgccagcttg tgtcttttca aggacaaagc ctgggatatg gcaggtacca
     1081 attgggttgc aagagctgag gtgattactg ttggaaagta tattaaagga cttgccatgc
     1141 cggaaagtct tcccaacgct tcaatacttg acttgtagtc aagtccgcct gccagaaacc
     1201 ttgcaggcaa aaggatggcc tccaccgccc ccataattga agtaagcagc ctgtttattg
     1261 atacggggat cgatcttttt gcaataacga ttgctgtctt tcttcttgaa agggttgctg
     1321 attttaaata tggctttttt gtttccttgt agaaaacaca acctaccacc aataggttgg
     1381 ccatttcccc aatggctgcc gataccgttg caagagcgca ggcataggcc agatcctgcc
     1441 ctgcaatctt tacagccaaa acaaaaataa ccccaatcct cacaatttgt tcaaccacct
     1501 gggatatggc agtaggcgtc acatgcatgg agccgtaaaa atatc
//
LOCUS       DAONVY010000522         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_54106_length_1533_cov_3.146820, whole genome shotgun sequence.
ACCESSION   DAONVY010000522 DAONVY010000000
VERSION     DAONVY010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54106_length_1533_cov_3.146820"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLI11_10885"
     CDS             complement(<1..180)
                     /locus_tag="PLI11_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53414.1"
                     /translation="MRAIGKAVLYVFAALLLIAAILLVSNEVIGRMYEKDFMRKREGV
                     ERPRAMELDNIIITSY"
     gene            complement(193..900)
                     /locus_tag="PLI11_10890"
     CDS             complement(193..900)
                     /locus_tag="PLI11_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53415.1"
                     /translation="MSNKCILLLFLTFAITIGLLVGCIEKASEGDATPTPVTTEEPTT
                     APTEEPTSEPTQEPKETIPPGTGQYAGGIASDEEVWALFYDENDSGDPTQVTGSMAAQ
                     FFATTEFDKLSVAFVTYGAPKDPPYHIYYYLFAWQGTYDDTLKSEPLVEEKKVNFNDW
                     ENVELSFDPLPDGEYLLYILNDESFTFIGVFNRVAGHESHRAFKDDQLWEDKTFSLRI
                     HYTKTPNNLYGPLSEPW"
     gene            1015..1527
                     /locus_tag="PLI11_10895"
     CDS             1015..1527
                     /locus_tag="PLI11_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53416.1"
                     /translation="MITVDQLKPYLMKMTDEESIYKKIYELRKEPDKFKEYMLNLDRK
                     ALLNQGIWLPKPSEEAGGWNFPEYLISNETQNIDVSKHNRYNPLFYHGHDVFEMIYIY
                     EGKCENTVEELPSKFVKMNMEKDDICILAPGIKHTMGVYDDDSIVINIIIRQSTFKRI
                     FFKIKISKLL"
BASE COUNT          497 a          322 c          268 g          446 t
ORIGIN      
        1 gtaggaagta ataattatat tatcaagttc cattgccctt ggcctctcaa caccttctct
       61 tttacgcata aaatccttct catacattct accaatcact tcatttgata caagcagtat
      121 tgctgcgata agcaataaag ctgcaaatac atacaatact gcttttccaa tcgctctcat
      181 cttcaatcat ccttaccacg gttctgacag cggaccatat aaattgttcg gcgtcttcgt
      241 gtaatgaatt ctaagactaa aagttttgtc ttcccacagc tgatcatcct tgaaagccct
      301 gtggctctca tggccagcaa ccctgttgaa tacaccaata aaagtaaagc tctcatcatt
      361 taagatatac aagagatact cgccatctgg gagtggatca aatgaaagtt caacattttc
      421 ccagtcattg aagtttacct ttttctcctc taccagtggc tcgcttttca aggtatcatc
      481 gtacgtcccc tgccatgcaa aaaggtaata gtagatatgg taaggtggat ccttgggagc
      541 accgtatgta acaaaggcca cacttaactt gtcaaactct gtagtggcaa agaattgagc
      601 tgccattgaa ccagtaacct gagtaggatc tccgctatca ttttcatcat agaacaacgc
      661 ccagacttct tcatcacttg caatacctcc tgcgtactgt ccggtgccag gcggtattgt
      721 ctcctttggt tcttgtgtgg gttcggaagt aggttcctca gtaggtgcgg tagtaggctc
      781 ctcagttgta acaggtgtag gagtagcatc accttccgag gctttctcaa tgcaacctac
      841 taataatcct atagttattg caaaagttaa aaataacaat aaaatacatt tatttgacat
      901 caaatcgcct cctgtatcaa gttaatttat tctagcatgt aaacttagta cgaaattaac
      961 aatctagttg acttttttta acccctttaa tatatttatt aacggagtga ttgcatgatt
     1021 actgtagatc agttaaagcc atacctgatg aaaatgacag atgaagagtc tatatacaaa
     1081 aaaatatatg aactaaggaa ggaaccagat aaattcaaag agtacatgct caatctggac
     1141 agaaaagcct tgctcaatca aggcatatgg ctgccgaaac cctcagaaga agcaggaggc
     1201 tggaattttc ctgaatacct aatcagcaac gaaacccaaa acattgatgt ttccaaacac
     1261 aacaggtaca atccactttt ctatcacggt catgatgtct ttgaaatgat atatatatac
     1321 gaaggcaaat gcgaaaacac tgtcgaagaa cttcctagta agtttgtaaa aatgaatatg
     1381 gaaaaggatg acatatgtat acttgcacca ggcatcaagc acaccatggg agtttacgat
     1441 gatgacagta ttgttataaa tattattatc aggcaatcga cattcaaaag aatatttttc
     1501 aaaatcaaga tatcgaaact tctataataa gtc
//
LOCUS       DAONVY010000523         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_54412_length_1527_cov_2.582880, whole genome shotgun sequence.
ACCESSION   DAONVY010000523 DAONVY010000000
VERSION     DAONVY010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54412_length_1527_cov_2.582880"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /gene="htpG"
                     /locus_tag="PLI11_10900"
     CDS             complement(<1..1014)
                     /gene="htpG"
                     /locus_tag="PLI11_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPZ53417.1"
                     /translation="METKSGYVSISTENIFPIIKKWLYSEKDIFIREIVSNACDAISK
                     MKKLVSIGEADLPDEEMNWKIEVLLDKKKKTITVYDNGIGMTEEEVLKYINQIAFSGV
                     KDFVEKYEGKTDESQIIGHFGLGFYSSFMVSKKVEIDTLSWVEGAEPIHWESNTGMDY
                     SISPSKKIDRRGTYVTMHIADDSKEFLDKYKLRETLRKYFSFLPVDLYFIDVNEKKDK
                     KEETEEDKPKPINDPNPLWLKNPNDCTDEEYKEFYKKVFMDFNDPLFWIHLNMDYPYN
                     LKGILYFPKLKLPYDSLEGTIKIFYNQVFVADNIKELIPEFLMLLKGVIDCPDLPLNV
                     SRSF"
BASE COUNT          387 a          304 c          297 g          539 t
ORIGIN      
        1 aaagcttctt gatacattaa gaggcagatc agggcaatcg attactccct tgagcagcat
       61 gaggaactca ggaatcaact ctttaatatt atccgcaaca aaaacctgat tgtagaaaat
      121 cttgatggtt ccctcaaggc tgtcataggg cagctttagt tttgggaagt agagtatgcc
      181 ttttaggttg tacggataat ccatgttgag gtgaatccaa aacagaggat cgttgaagtc
      241 cataaatacc tttttgtaga actccttgta ttcctcgtcg gtgcagtcgt ttggattctt
      301 caaccaaaga ggattcggat cattaattgg ctttggcttg tcttcttctg tttcttcctt
      361 tttgtccttc ttttcattga catctatgaa atacaggtca acaggcagga aagaaaagta
      421 ttttctcagt gtctctctta gtttgtattt atcgagaaac tccttgctgt cgtctgctat
      481 gtgcattgta acatatgttc cgcgcctgtc gatttttttc gaagggctta tggaatagtc
      541 catgcctgta ttgctttccc agtgaatggg ttctgctcct tccacccagg acagggtgtc
      601 tatttccacc tttttggaca ccatgaagga agagtagaaa ccaagaccga aatggccaat
      661 gatttgtgac tcgtcggttt ttccttcata tttctcaaca aaatctttca cgccggaaaa
      721 agcaatctgg tttatatact tcagaacttc ttcttctgtc atgccgatgc cgttgtcata
      781 aaccgtaatg gttttctttt tcttgtccag caggacttct attttccagt tcatttcctc
      841 atcaggcaaa tccgcttcgc ctatggaaac cagttttttc atcttgctta tggcgtcgca
      901 tgcgtttgat acgatttccc tgatgaaaat gtccttttct gaatagagcc attttttaat
      961 aattgggaat atattttcag tgcttataga tacataacca ctctttgttt ccattcttat
     1021 cctcctcagc tgttatactc tcaaaaagaa attatatact aacagctgat aaaaatcaaa
     1081 tatgaaggtg ttgtgtacat gtgcatatgt ttcctggcct tgattgcagt agaatatttc
     1141 aacttttgtg cggtatttgc gggaaaggac gtctgctaac aataatgcac tccgggatct
     1201 tcatggtatg ataccttgtt tgtggcgtat acgacaacct tgtaaaatga tggatcagag
     1261 atttctttca tttccccctg catggttgat tcatcgagca aaaagttaat ctcgtcttcg
     1321 gttttgctgc cgaataaggt ttaccatttg cccgggtatc aaggacttcc tttttaaata
     1381 cttttgtatt caatttgcga ttgcatgtca tgggtcttat atcatacaaa tcgtttttat
     1441 agataaggta tggtttcaca taaaaaatca aactgcttct ttccgtttgc atctccttgt
     1501 ggtataattg ctttgtagaa ggggttg
//
LOCUS       DAONVY010000524         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_54706_length_1521_cov_3.435198, whole genome shotgun sequence.
ACCESSION   DAONVY010000524 DAONVY010000000
VERSION     DAONVY010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54706_length_1521_cov_3.435198"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLI11_10905"
     CDS             complement(<1..524)
                     /locus_tag="PLI11_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPZ53418.1"
                     /translation="MSLIIVLSACGAGKDAINVVSRESGSGTRGAFIELFGIEEKDAS
                     GNKIDKTTVEAVVLSGTQQVMSYIAGDVNSIGYISLGSLNDTVKAVKIDGVEATVENI
                     KNGKYKVARPFNIATKGELSDATQDFINFILSEDGQKVVEDAGFISKENTGPFTSSNS
                     DGKIVVSGSSSVTPV"
     gene            complement(676..>1521)
                     /locus_tag="PLI11_10910"
     CDS             complement(676..>1521)
                     /locus_tag="PLI11_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPZ53419.1"
                     /translation="YSGSSGNCIFVGTDKTRILIDAGLSGVAIISALKSIGEDPANIN
                     GILVTHEHSDHIKGVGILSRKFDIPIYANIKTWEQMEPCIGNVKDKNIRIIEKETRKP
                     HQCSLLEYVGESEEVEECTSYKSFEIGDIGIVCWPISHDAKEPVSYALYMDNMKISVA
                     TDMGYASDVVRDNLKGSDLLFIESNHDVEKLKKGRYPWSLKKRILSDVGHLSNEACAS
                     VVRECVCEGTKQVVLGHLSQENNFPHLAYNASKNMLDDIGCVIGRDMYLSVAKRDEVS
                     DVILL"
BASE COUNT          422 a          387 c          244 g          468 t
ORIGIN      
        1 acaggggtta ccgatgagga accggaaacc acaatctttc catctgaatt gctgctggta
       61 aaaggacctg tattctcttt gctaatgaat cctgcgtcct ccaccacctt ctgaccgtcc
      121 tcggacagaa tgaaatttat aaagtcctgt gtggcatcac taagttctcc cttggtcgca
      181 atgttaaacg gccttgccac cttgtatttc ccattcttga tattctctac agttgcctcc
      241 actccgtcta tttttacagc cttcaccgta tcattgagtg aacctagtga gatgtatccg
      301 atggagttta catctcctgc aatgtagctc attacctgct gggtaccgct aagaaccaca
      361 gcttcaacag ttgttttgtc aatcttgttt ccggacgcat ccttctcctc gataccaaaa
      421 agttcgataa atgcacctct tgttcctgaa ccgctttctc ttgacaccac attgattgca
      481 tcttttcccg caccgcaggc agacagaaca ataatgagtg acatgacaac caataagata
      541 gtttttctaa acatatttcc tcctccttta ataaaaaaat tgtacatgaa acaagctttt
      601 tggcttatct cacgtacaat tatatttccc caaaatatag ggcaaatatg agtattgtaa
      661 aaaccgtgta aaatactaaa ggagaatcac atcgcttacc tcatctcttt tggcaacgga
      721 caaatacata tccctgccaa ttacacatcc aatgtcatcc agcatgtttt tagacgcatt
      781 gtatgcaagg tgtgggaaat tgttttcctg actcaaatga cccaaaacca cctgctttgt
      841 gccttcacag acacactctc tgacaactga cgcacatgct tcatttgaca aatgtccaac
      901 atcactcaat atccttttct taagagacca aggatatctt ccttttttca gtttctccac
      961 atcatgattt gattcaataa acagtaaatc agagcccttt agattatccc tgaccacatc
     1021 cgatgcatat cccatatcgg ttgctacgga aatcttcata ttgtccatat acaaggcata
     1081 acttacaggt tcttttgcat cgtgggagat gggccagcaa acaattccaa tatcccctat
     1141 ctcaaaggac ttgtaagatg tgcactcttc cacttcttca gactctccca catattccag
     1201 caaggagcac tgatgtggct ttctagtctc tttctcaatt attctaatat tcttgtcctt
     1261 tacatttcct atacacggct ccatctgttc ccatgtcttg atgtttgcat aaatagggat
     1321 atcaaattta cgagagagta tccccactcc tttaatatga tccgaatgct catgggttac
     1381 cagtattccg ttaatattgg caggatcctc cccgatactt tttagcgcac tgattatggc
     1441 aacaccggac aaacccgcat ctatcaaaat gcgggtttta tcagtaccta caaaaataca
     1501 attgccgctg ctgccggaat a
//
LOCUS       DAONVY010000525         1518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_54864_length_1518_cov_3.635680, whole genome shotgun sequence.
ACCESSION   DAONVY010000525 DAONVY010000000
VERSION     DAONVY010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54864_length_1518_cov_3.635680"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /gene="serS"
                     /locus_tag="PLI11_10915"
     CDS             complement(<1..1128)
                     /gene="serS"
                     /locus_tag="PLI11_10915"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPZ53420.1"
                     /translation="MIDIRLIRKDPDLVRENIKKKFQFEKLPLVDEVIELDAKYREAK
                     LRSDNLRSERNTISKQIGTLMREGKREEAEKIKSQIGKMAKQLEESERLEKEYFEKVQ
                     EIMLQIPNIIDDTVPIGKDDSENVEVERFGEPVVPDFEIPYHVDIMERLDGIDLDSSR
                     KTSGSGFYYLKGNIARLHSAVLSYARDFMIDKGFTYYIPPFMIRGNVVSGVMSFAEME
                     NMMYKIEGEDLYLIGTSEHSMIGKFIDTILDEESLPQTLTSYSPCFRKEVGAHGIEER
                     GVYRIHQFEKQEMVVVCKPEDSKAWFDKLWQYAVEFFRSLDIPVRVLECCSGDLADLK
                     VKSVDVEAWSPRQQKYFEVGSCSNLGDAQARRLGIRVRGKDG"
BASE COUNT          388 a          366 c          261 g          503 t
ORIGIN      
        1 accatccttg cctcttacac gaattccaag ccttcttgcc tgagcatcac ccaggttgga
       61 gcagcttcct acttcaaaat atttctgctg tcttggagac caggcttcca cgtcaacact
      121 cttgacttta agatctgcaa ggtcgccgga gcagcattcc agaaccctta cagggatgtc
      181 tagagatctg aagaactcta cagcatactg ccaaagtttg tcaaaccacg ctttactgtc
      241 ctcaggcttg cagacaacaa ccatttcctg cttttcaaac tgatgaattc tgtatactcc
      301 cctctcttct atgccatgag ctcctacttc ctttctgaaa cagggtgaat agctggtaag
      361 cgtttgagga agggattctt catcaagtat tgtgtctata aacttgccta tcatggagtg
      421 ctcgcttgta cctattaaat acaagtcttc tccttcaatc ttgtacatca tgttttccat
      481 ctcggcaaag ctcatgactc cactgaccac attgccgcgt atcatgaaag gcggaatgta
      541 gtaggtaaag cctttgtcta tcatgaagtc tcttgcatag gaaagtacag ctgaatgcag
      601 tcttgcaata tttcctttta gataatagaa gccgctgccg gatgtctttc ttgagctgtc
      661 caggtcaata ccgtcaagcc tctccattat gtccacatgg tatggaatct caaaatcagg
      721 cacaaccggt tcgccaaaac gctccacttc cacattctca gaatcatcct tgcctatggg
      781 taccgtatca tctatgatat tgggaatctg aagcattatc tcctggactt tttcaaagta
      841 ttccttctca agtctttccg actcttccaa ttgctttgcc atttttccaa tctggctctt
      901 tatcttttct gcttcttctc tttttccttc cctcatcaat gtgcctattt gcttgcttat
      961 tgtgtttctt tcacttctta aattgtcgct tcttaatttt gcttctctgt atttggcgtc
     1021 aagttcaatt acttcatcta ccagaggaag cttttcaaat tggaacttct ttttaatgtt
     1081 ttctcttacc aaatcaggat cttttcttat caatctaata tctatcattt aaaaacctcc
     1141 ttgagataaa aataaccttc aatcccgaaa atgggacgga aggttccgcg ttgccaccca
     1201 aattgtcata aatgtattat gaccacttgc acggtacttt aaagggtacc aaccttcagc
     1261 tttcactgat tgctcgagaa gtggaaaggt ttgtgttctt tccgacttgc accaaccgtc
     1321 ggctctcttt gaagaaggga caaaccattt tcttccgtca tagcaaacaa acttatttct
     1381 tttggatgat tatatatcat tttggagaaa taatcaagtt aataaaaagg accacattaa
     1441 gggataagtt actaggattt gatggatcat acgtgccttt tgcattgctt acgtccccat
     1501 acagggatag attgcgac
//
LOCUS       DAONVY010000526         1518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_54885_length_1518_cov_2.448394, whole genome shotgun sequence.
ACCESSION   DAONVY010000526 DAONVY010000000
VERSION     DAONVY010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54885_length_1518_cov_2.448394"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLI11_10920"
     CDS             complement(<1..1001)
                     /locus_tag="PLI11_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPZ53421.1"
                     /translation="MGLFEKLFGTSSERAVKQIMPIVKAVNELEPRMEKLSDEELRSL
                     TAKYKERFANGESLDDLLVEAFATVREAAKRVLGQRHYDVQIIGGVVLHQGRIAEMKT
                     GEGKTLVSTLPAYLNALTGNGVHIVTVNDYLAKRDRDWMGKIYNFLGLSVGCIIHGLT
                     QKERQDAYNCDITYATNNELGFDYLRDNMVIYKEQLVQRELNYAIIDEVDSILIDEAR
                     TPLIIAGSSEKPSDMYEIANTFVRTLSKKVIKETDSKDLNEDVTEDYIVDEKARTATL
                     TPYGVKKAESFFNLENLADPENATVVHHINQALKAYGIMKRDIDYVVKDGSVLIVDE"
     gene            complement(1007..1303)
                     /locus_tag="PLI11_10925"
     CDS             complement(1007..1303)
                     /locus_tag="PLI11_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HPZ53422.1"
                     /translation="MNKKIRSKQLDDLFRAILSLKTIDECYRFFEDIGTISEIQAFSQ
                     RFEVARMLKEGYKYTEIAQATGASTATISRVSKSLNYGADGYNIVIERLKKEND"
     gene            complement(1371..>1518)
                     /locus_tag="PLI11_10930"
     CDS             complement(1371..>1518)
                     /locus_tag="PLI11_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPZ53423.1"
                     /translation="EEVKAGNKEFLQAKESIYNVLVDMNLPELEYFMEHFLPSYSLSL
                     DEMN"
BASE COUNT          396 a          376 c          251 g          495 t
ORIGIN      
        1 aactcgtcaa ctatcagtac gctgccatcc ttgacaacgt aatctatatc cctcttcatg
       61 attccatagg ccttaagcgc ctgatttata tgatgcacca ctgttgcatt ctcaggatcc
      121 gccagattct caaggttgaa aaagctctcc gcctttttaa caccataagg agtaagggtt
      181 gcggtccttg ccttctcgtc tacaatatag tcttccgtta catcttcatt caagtccttg
      241 ctgtcggttt ccttgattac ctttttagac agagtcctta caaaggtatt cgcaatttca
      301 tacatatcgc tgggcttttc acttgaccca gctattatca aaggagttct tgcctcatct
      361 ataaggattg agtccacttc gtcaatgatt gcataattaa gttctctttg gacaagctgc
      421 tccttgtaaa tgaccatatt gtctcttaga tagtcaaaac ccagttcgtt gttggtagca
      481 taggtaatgt cgcagttata tgcatcctgt ctttcctttt gggtaagacc gtgtatgata
      541 cagccaacac tgagcccaag aaagttatat atctttccca tccagtccct gtctcttttg
      601 gcaagatagt catttacagt aactatatgg acgccatttc cagtaagcgc attcaaatac
      661 gcaggcaatg ttgaaaccag ggtttttcct tcacccgtct tcatttctgc aatacgaccc
      721 tgatgcagaa caacgccgcc gattatctgc acatcataat gtctttgtcc aagtaccctt
      781 tttgcagcct ccctcactgt agcaaaggct tcaaccagca aatcatccaa agattcgcca
      841 tttgcaaacc tctctttata ctttgcggta agacttctaa gctcttcgtc agacaatttt
      901 tccatccttg gctctagctc attaacggcc ttgacaatag gcataatctg tttgactgcc
      961 ctttcactgg aggttccaaa gagtttctca aataatccca tattgactaa tcattctcct
     1021 tttttaatct ttctataaca atattgtaac catcagcacc ataattaaga cttttgctga
     1081 ctctgctgat ggtggctgtg ctggcaccgg tagcctgtgc aatctcagta tacttgtagc
     1141 cttccttaag cattcgggct acctcaaagc gctggctgaa tgcctgtatc tcgcttatag
     1201 tgccaatatc ctcaaagaac ctgtagcatt cgtctatggt tttcaaagac aatattgctc
     1261 tgaagagatc atccaattgc ttgcttctaa tctttttatt cacacaaaac taccttcctt
     1321 tgaaaatata acatcttgta ttttaattca ttaaagtgca aatgtcaaag tcagttcatc
     1381 tcgtctagag acagactgta cgatggcagg aaatgctcca tgaaatattc aagctcagga
     1441 agattcatgt caacaagcac attatatatc gattcctttg cctgcaaaaa ctccttgttt
     1501 cccgccttta cttcttcc
//
LOCUS       DAONVY010000527         1513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_55114_length_1513_cov_3.325103, whole genome shotgun sequence.
ACCESSION   DAONVY010000527 DAONVY010000000
VERSION     DAONVY010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55114_length_1513_cov_3.325103"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLI11_10935"
     CDS             complement(<1..830)
                     /locus_tag="PLI11_10935"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HPZ53424.1"
                     /translation="MRYYLVVIDSFGIGADSVCGEYGDCGSNTALSASRAIEGEKWRF
                     LVRMGLGNSCKTLGVELEGCEEVDNPIANYAVLEKRGGGKDTQTGHWELAGMNLDFTL
                     TIFPPEYPSFPEELVKRLEKETGRKVIGNKSVSGTKIIKELGKEHMETGSIICYTSAD
                     SVFQIAAHEEVVPLEELYDICEKARRICDDYKVGRVIARPFVGNEKDGFVRTKNRKDY
                     SIELPNKTLMEDKLHSLDVSVVAVGKIWDIFDGKGIDVNYPDKGNEACLDRVEKIART
                     "
     gene            complement(849..>1513)
                     /gene="recO"
                     /locus_tag="PLI11_10940"
     CDS             complement(849..>1513)
                     /gene="recO"
                     /locus_tag="PLI11_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPZ53425.1"
                     /translation="EGDKILTVFSDTNGIISVRARRAKRTGYRYLASVQPFAYSSFEL
                     YKGRSMYSLNSAQLIEPFFNLRNDLDVLFTAGDMARICLTMIQQEMPEPESLRLLLNC
                     LHFLNTGKMDYKLVYDIFRLRMLTLQGFAPVISSCGICERKPEGQDLVFSVAEKSIFC
                     SECAKSNMVDNVELDYDTLAAILYICNSKLEKVFSVSVSDNVEQLLDYISGMLVKSCL
                     EK"
BASE COUNT          388 a          410 c          250 g          465 t
ORIGIN      
        1 cttgtccttg caattttctc caccctgtca agacatgcct cgttcccctt gtccggataa
       61 tttacatcaa tgcctttgcc gtcgaaaatg tcccatatct ttcccacagc aacaacactt
      121 acatccaaag aatgaagctt atcctccata agtgtcttgt tggggagttc aatactgtag
      181 tcttttctat tcttcgtcct tacaaaacca tccttttcat tccctacaaa cggtcttgca
      241 atcactcttc ccactttgta atcatcacat attctccttg ccttctcgca tatgtcataa
      301 agttcttcca gaggaaccac ctcttcatga gccgctatct gaaacacgga atccgcagac
      361 gtatagcaga ttattgaacc tgtctccata tgctcctttc caagctcttt tatgattttc
      421 gttcctgaca cactcttgtt gccaattact tttcttccag tttccttttc aagccttttt
      481 acaagctctt caggaaatga tggatattcg ggcggaaata tggtcaatgt gaaatcgagg
      541 ttcatgcctg caagctccca gtgtccggtc tgggtatcct tgccgcctcc tcttttttca
      601 agcaccgcat aatttgcaat tggattgtca acctcttcac atccctcaag ctcaacccca
      661 agggttttgc atgaatttcc aaggcccatt ctgacgagaa atctccattt ttcaccctct
      721 atcgccctgc ttgcagacag tgcggtattt gaaccgcaat caccatactc cccgcaaaca
      781 ctgtcggctc ctattccaaa gctgtctatg acaacaagat aatatctcat aaatatacct
      841 ctttcccttt atttctcaag acaggacttt acaagcatcc cgcttatgta atccagaagc
      901 tgctcaacat tatcggaaac tgacacgctg aacactttct ccagctttga attgcatatg
      961 tacaatattg cagccaacgt gtcataatcc agttccacgt tatctaccat attgcttttt
     1021 gcacattcag agcagaatat ggatttttcc gccacagaaa aaacaaggtc ctgcccttct
     1081 ggttttctct cgcaaattcc acagctcgat ataaccggtg caaaaccctg cagtgtcagc
     1141 attctaagcc tgaaaatgtc gtaaacaagc ttgtaatcca tttttcctgt attcagaaaa
     1201 tgtaaacagt ttaaaagaag ccttagactt tcaggctctg gcatttcctg ctgtatcatt
     1261 gtcaagcaga ttcttgccat gtctccggcg gtaaacaaca catccaggtc attcctcagg
     1321 ttgaaaaatg gttcaatcag ctgggcacta ttcaaactgt acatgcttct tcccttgtac
     1381 agctcaaaag agctgtaggc aaagggctgt acagaggcaa gatacctgta accggttctt
     1441 tttgcgcgcc ttgccctgac actgataatt ccatttgtgt cggaaaacac ggtgagaatc
     1501 ttgtcgccct ctc
//
LOCUS       DAONVY010000528         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_55335_length_1509_cov_1.878267, whole genome shotgun sequence.
ACCESSION   DAONVY010000528 DAONVY010000000
VERSION     DAONVY010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55335_length_1509_cov_1.878267"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PLI11_10945"
     CDS             complement(<1..855)
                     /locus_tag="PLI11_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53426.1"
                     /translation="MNRRRSIHSVLAFVMIFSILASFIIIKTPQVTAEVSKPVTYAKF
                     TSELTDVGQPRESGVTYTVNPGTAWVYQDDSVSFDRDKKAVKYESTTDNATSWIDLYF
                     DREYKTTGDDYWITVLFQEESDKNFHPNRGYQTLNYSFGVHTTQESRTTWSYSTIDIN
                     GETYKLAYTKISGFAENKPVTGFRIYPRQGIYYFYSAAIYTSEQDALAYAGMLPIHEE
                     NEILAYGLYNTETDCKGIKPSGAVPPVDAGTVWGAPESASASFNHDLKGATITSKASQ
                     ETWGALFFN"
     gene            complement(852..>1509)
                     /locus_tag="PLI11_10950"
     CDS             complement(852..>1509)
                     /locus_tag="PLI11_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPZ53427.1"
                     /translation="VDAGTVWGAPESESVSFNEELKGATVTSKKDSQTWAALHFNEEF
                     TTAGDGYVEAILYQNTSNAAFSSGASYQLSNYQFGIHADTDKRVNFRYAQVTIDGQEY
                     MLAYSAVENMPADLKVTGIRAQLSSNQSYTFIAAAVLKNDADMDMLFEDILTQPEHTE
                     PEPTTEPPTEPTEPPTEPATNPETSDSGYSIAMLFVAVSFILLILVKRRQNDTRSEKA
                     "
BASE COUNT          406 a          333 c          295 g          475 t
ORIGIN      
        1 gttaaagaac aatgctcccc atgtttcctg agacgccttg gaggtaatag tagccccttt
       61 aaggtcatga ttgaaggacg cagaagcgct ttcaggagca ccccatacag ttccagcatc
      121 cactggcgga accgcaccag aaggcttgat acccttgcaa tctgtttccg tattatatag
      181 gccgtaagct aaaatctcat tctcttcatg gattggaagc attcctgcat acgccaacgc
      241 atcctgctca cttgtataaa tagctgcaga ataaaagtaa tatatgccct gccttggata
      301 tatgcgaaat cctgtcacag gcttgttttc tgcaaacccg ctgatttttg tatatgcaag
      361 tttgtatgtt tcgccattaa tatcaatagt gctataactc caagttgtcc ttgattcttg
      421 cgttgtgtgt acaccaaagg agtaatttaa tgtttgatat cctctgtttg gatgaaagtt
      481 tttatcgctt tcttcctgaa acagaactgt aatccaataa tcatcaccgg ttgtcttgta
      541 ttccctgtcg aagtacagat caatccatga tgtagcatta tccgttgtag attcgtattt
      601 taccgctttt ttatccctgt caaaagaaac actgtcatcc tgatacaccc atgcagttcc
      661 tggattaaca gtatatgtaa caccagattc tctgggctgg ccaacatctg tcaattccga
      721 ggtaaattta gcatatgtaa caggttttga tacttctgcc gtaacttggg gtgttttgat
      781 tataatgaaa gacgccaata tggaaaatat catcacaaag gccaaaacac tgtgtattga
      841 tctccgtcta ttcatgcttt ctcactcctt gtgtcatttt gccttctctt aacaagtatt
      901 agtaaaataa acgatactgc aacaaacaac atggctatgg aataacctga atccgatgtt
      961 tctggattgg ttgctggttc tgtaggtggc tcagttggtt cagttggcgg ttctgtagtt
     1021 ggctcaggtt ccgtgtgttc tggctgagtc aaaatatctt caaagagcat gtccatgtcc
     1081 gcatcgttct ttaacacggc tgctgcgatg aaagtataac tctgattgga tgaaagttgg
     1141 gcacgtatgc ctgttacttt caaatctgca ggcatatttt cgacagcaga ataagcaagc
     1201 atatattcct gcccatcaat tgttacctgc gcataacgga aatttactct cttatccgta
     1261 tccgcatgta taccaaactg atagttgcta agctgataag atgcaccaga gctaaaggca
     1321 gcatttgatg tgttctgata caaaattgct tcaacatagc catcgcctgc tgttgtaaat
     1381 tcttcgttaa aatgaagggc tgcccaagtt tgcgagtcct tttttgaagt gacagtagct
     1441 cccttcaatt cctcattaaa ggaaacactt tcgctttcag gagcacccca tacagttcca
     1501 gcatccact
//
LOCUS       DAONVY010000529         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_55528_length_1505_cov_4.202759, whole genome shotgun sequence.
ACCESSION   DAONVY010000529 DAONVY010000000
VERSION     DAONVY010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55528_length_1505_cov_4.202759"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="dapA"
                     /locus_tag="PLI11_10955"
     CDS             <1..714
                     /gene="dapA"
                     /locus_tag="PLI11_10955"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPZ53428.1"
                     /translation="GEHLDVIRYAVEKVAKRVPVIAGTGSNDTRHAVKLSTEAEKLGA
                     DALLSVTPYYNKTTQAGLYEHFKAIAKSVSIPIILYNVPSRTNLNIDPATYEKLCKIP
                     NINAVKECNLMQVPETVKRCGDELNLYSGEDGNVYFLLAAGGLGVISVMANIIPKFTH
                     ELVASYFRGETEKSWKMQLQCLDLVKALFCEVNPIPVKEALNYMGFNVGQCRMPLVGM
                     SEKNREFLIRELNNFGING"
     gene            778..>1505
                     /gene="dapB"
                     /locus_tag="PLI11_10960"
     CDS             778..>1505
                     /gene="dapB"
                     /locus_tag="PLI11_10960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPZ53429.1"
                     /translation="MTRILLSGANGRMGQAIVKLASNRNDCKIVAGIDINTNVKNDFE
                     VYASFSEVPEDLDVDVIIDFTNPSLLIPLADFARDRKTPLVVATTGLSKQDMEYLQET
                     AKTTSVLYSANMSLGICLLKDLVKRAVLFLGEGFDIEIIEKHHNQKLDAPSGTALALA
                     DSINAVDNNKYEYIYDRHSRKAKRSPREIGFSSIRGGNIVGDHDVIFAGNNEIIEISH
                     KALSRDIFADGALRAALFLKDNEKG"
BASE COUNT          513 a          289 c          341 g          362 t
ORIGIN      
        1 ggtgaacatt tggatgtaat tcgttacgca gtggaaaaag tggcaaaaag ggttcctgtt
       61 atagctggta ctggcagcaa tgacacaagg cacgctgtaa aactaagcac agaagcagaa
      121 aagctaggcg cagatgctct tttgtctgtt acaccctact acaacaagac aacacaggca
      181 ggactttatg agcacttcaa agcaattgca aagagcgtgt caatacccat aatactctac
      241 aatgtgccca gcagaacaaa cctgaacata gaccctgcaa catatgaaaa gctttgcaag
      301 atcccaaaca tcaacgcagt gaaggaatgc aatcttatgc aggtgcctga aacagtaaag
      361 agatgtggcg acgagctcaa cctctattcc ggagaagatg gaaacgtcta cttcctgctt
      421 gcagctggtg gccttggagt tattagtgtt atggcgaaca taattccgaa gtttactcac
      481 gagctggtag ccagctattt tagaggagag acagagaaat catggaagat gcagcttcaa
      541 tgtcttgatc ttgtaaaagc gcttttctgt gaagtaaatc ccatacctgt aaaagaagcc
      601 ttgaactaca tgggatttaa tgttggtcaa tgccgcatgc ctctggttgg tatgagtgag
      661 aagaacaggg aattcctcat aagggaactc aacaattttg gcataaacgg ataagtacat
      721 taaaatacgg aaaacaccaa gttttccgta ttattatata tcaaaggaga atctgacatg
      781 acaagaatac tcttaagcgg agcaaacggc agaatgggac aagcaatcgt aaaactggct
      841 agtaacagaa acgactgcaa aatcgtagca ggaattgaca tcaacacaaa tgtaaaaaat
      901 gattttgaag tgtacgccag tttttcagaa gttcctgaag atctggatgt tgatgtaatt
      961 atagacttta caaacccttc cctattgata ccgctggcgg actttgcaag agacaggaaa
     1021 acacctcttg tggtggcaac caccgggtta tccaaacagg acatggaata tttgcaggaa
     1081 acagcaaaaa caacaagtgt actttactcg gcaaacatgt cccttggcat atgcctgctt
     1141 aaagacctgg taaaaagagc tgtattgttc ctgggtgaag gttttgatat tgaaatcatt
     1201 gagaagcatc acaaccagaa gcttgacgca cctagtggaa ctgctttggc tcttgcagat
     1261 agcataaatg ctgtcgataa caacaagtat gagtatattt atgacagaca ttcaagaaaa
     1321 gcaaaaagaa gcccaagaga aataggcttc agttcgataa gaggcggcaa tatcgtagga
     1381 gatcatgatg taatatttgc cggcaataat gaaattattg aaataagcca caaggccttg
     1441 tcaagagaca tttttgcgga tggtgcccta agggctgcac tgttcctcaa ggacaatgaa
     1501 aaagg
//
LOCUS       DAONVY010000530         1503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_55649_length_1503_cov_3.151243, whole genome shotgun sequence.
ACCESSION   DAONVY010000530 DAONVY010000000
VERSION     DAONVY010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55649_length_1503_cov_3.151243"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLI11_10965"
     CDS             <1..206
                     /locus_tag="PLI11_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPZ53430.1"
                     /translation="YGYVKRPSEIHVKALNENGEEIEIKANGMLANILSHELDHLDGV
                     LYKDKAYEFAKLEDDEELSDEDE"
     gene            210..1151
                     /gene="fmt"
                     /locus_tag="PLI11_10970"
     CDS             210..1151
                     /gene="fmt"
                     /locus_tag="PLI11_10970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPZ53431.1"
                     /translation="MNKRLKIVFMGTPEFALPSLKALDVHHDVVCVVTNPDKPKGRGG
                     KVTSPCTKVYAESKGIDVLQPESVKAKEFYDALTSYGADVFVTCAYGKILPESIISIP
                     KFGCINIHASLLPKYRGAAPLWHCIIDGEKETGITTMMTDVGMDTGDILLAREIDIPV
                     DMTMGELHDVLAELGSVVIIETIDKLMDGTLKRIPQNHDEAIYAPMVTRETGRIDFKK
                     SALDVHNLVRGTNPFPGSYCFVDGKKLKIWKTALVDMDVEDARPGEVVEIDQTGALVK
                     AGLGVVRIIEVQGENAKRMHVWDYNNGHPMPPGTILN"
     gene            1162..>1503
                     /locus_tag="PLI11_10975"
     CDS             1162..>1503
                     /locus_tag="PLI11_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPZ53432.1"
                     /translation="MKKTNLLDYTLDELKEYVSGLGLPKYRAAQIRSNIYKGVFEFSK
                     MTDLPVETREKLDNVACTGRLEIVRKLESNIDGTKKYLFDLGDGNVIESVLMKYKYGY
                     SACISSQVGYKM"
BASE COUNT          486 a          239 c          390 g          388 t
ORIGIN      
        1 attacggcta tgtaaaaaga ccgtctgaga tacatgtaaa ggcattgaat gaaaatggtg
       61 aagagattga gataaaggca aacggaatgc tagcaaacat attaagtcac gagcttgacc
      121 atttggatgg agttctctac aaagacaagg catatgaatt tgccaagctt gaagatgatg
      181 aagagctgtc tgacgaggat gagtgattga tgaataaaag attgaaaatt gtattcatgg
      241 gtactcctga gttcgccctg ccttccctga aagctttgga tgtgcatcat gatgtggttt
      301 gcgttgtcac aaacccggac aagccaaagg gcaggggagg aaaagtaacc agtccgtgta
      361 caaaggtgta tgcagagagt aaagggattg atgttctgca gcctgagagc gtgaaggcaa
      421 aggaatttta cgatgccttg acatcctatg gtgcggatgt ttttgtgaca tgcgcttatg
      481 gaaagatact acctgagagt attatctcaa taccgaagtt cgggtgcata aacatacatg
      541 catccctttt gccaaagtat aggggggctg ctcctctttg gcactgcata atagatggag
      601 aaaaggaaac tggaattacc accatgatga cggatgtggg catggatacg ggtgatattc
      661 tacttgccag agagattgac attcccgttg acatgaccat gggagagctg catgatgtgc
      721 tggcggagct tggcagtgtt gttatcatag aaacaattga taagcttatg gatggaactc
      781 ttaaaaggat acctcagaac catgacgagg caatttatgc tcccatggta acaagggaaa
      841 cgggaagaat agatttcaaa aaatccgctt tggatgtgca taatcttgtt aggggcacaa
      901 atccctttcc aggaagttat tgctttgtag atggcaagaa gcttaagata tggaagacag
      961 ctcttgttga tatggatgtt gaagatgcaa gacctggaga agttgttgaa atagatcaaa
     1021 caggtgcatt ggtgaaagcg ggtttgggtg ttgtcagaat tattgaagta cagggagaaa
     1081 atgcaaagag aatgcatgtc tgggattaca acaacggaca tcccatgcct cctggaacaa
     1141 tattaaattg aaggtgaaaa tgtgaagaag acaaaccttc tcgattatac attggatgaa
     1201 ctaaaagaat atgtatctgg actgggtctt cccaaataca gggcagcaca gataaggagt
     1261 aatatataca agggtgtttt tgaattttcc aagatgacgg atcttccagt tgaaacaagg
     1321 gagaaacttg acaatgttgc atgtactgga aggcttgaga tagtaaggaa gctggaatca
     1381 aatatagatg gaactaaaaa gtatcttttt gatctgggag acggaaatgt aattgaaagt
     1441 gttctcatga agtacaaata cggctattcc gcctgcatat cctcacaggt tggctacaaa
     1501 atg
//
LOCUS       DAONVY010000531         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4188
            NODE_55799_length_1500_cov_3.806228, whole genome shotgun sequence.
ACCESSION   DAONVY010000531 DAONVY010000000
VERSION     DAONVY010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866159
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:08:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,197
            CDSs (total)                      :: 2,174
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 8 of 21
            Pseudo Genes (incomplete)         :: 11 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55799_length_1500_cov_3.806228"
                     /isolate="AMR_MDS_4188"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLI11_10980"
     CDS             complement(<1..472)
                     /locus_tag="PLI11_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPZ53433.1"
                     /translation="MKKLGLLLVLSLMCLALTISSFAAVDSYSQNFESYEAGTEIKDD
                     LLPDWSLIRATPTGSFIREEGGNKYFAIDAYYHAEYTKKLTEPYVYEVDVYNPDAKFN
                     VSAFFVRAGSERFDVMFGKSTGYEWDGDGNSGFPSVGGSGLLVLPNNGNKLSLVV"
     gene            complement(517..1482)
                     /locus_tag="PLI11_10985"
     CDS             complement(517..1482)
                     /locus_tag="PLI11_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPZ53434.1"
                     /translation="MKLGVLTVLLSDMSVRDAMAFLAKEGVQSVEIGCGGFPGKAHCN
                     PEELLKDEKALEEFKDAIYSNGLEISALACHGNPVHPDKEIAKQYHDDFINAILLAEK
                     LGVEKIIAFSGCPGDSPDSKYPNWVTCPWPEDFLKILDYQWNEVLVPYWKETGKFALE
                     HGVTKICFEMHPGFCVYNPETLLRLRAEVGEVIGANFDPSHLIWQGMDPVEAIRELGD
                     AIYHVHGKDVRIDEANVKKNGVLDTKHYGDVLNRAWVFRTMGYGNDYKLWKDMISMLK
                     TVGYDDVISIEHEDSLMSPQEGLRKAISFLKEVLVFEKPGAMWWA"
BASE COUNT          407 a          387 c          262 g          444 t
ORIGIN      
        1 ttacaacaag actcaacttg tttccattgt tgggcaatac aagaagtcca ctgcctccaa
       61 cggatggaaa tcctgagttg ccatcgccat cccattcata tcctgtgctc tttccaaaca
      121 tgacatcaaa tctttcactg ccagcgcgta caaagaacgc tgaaacgttg aacttagcat
      181 caggattgta tacatcaact tcgtatacat acggttcggt aagctttttc gtgtattctg
      241 catggtaata tgcatcaatc gcaaaatatt tgttgccgcc ctcttccctg ataaaggatc
      301 cggttggtgt tgctctaatt aatgaccagt ctggcaagag gtcgtcctta atctcggttc
      361 ctgcctcata gctctcaaaa ttctgcgagt agctatctac agctgcaaaa gatgagattg
      421 taagagctaa acacattaat gaaagtacca ataaaagtcc taattttttc atgataaacc
      481 tcctttagag taataatcta tgccaaggca gcatatttat gcccaccaca tggcaccagg
      541 tttttcaaat accaacactt ctttaaggaa ggatatggcc tttctaagac cttcctgagg
      601 agacataagg ctgtcctcat gctctatact tataacatcg tcataaccta ctgtcttaag
      661 catgcttatc atgtctttcc acaatttata gtcgtttcca taacccatgg ttctgaatac
      721 ccatgcacgg tttaacacat ctccatagtg cttggtgtca agtactccgt ttttcttgac
      781 atttgcctca tctattctga catccttgcc atgaacatgg taaatagcat ctccaagctc
      841 ccttatagcc tctacagggt ccattccctg ccaaatcaga tggcttgggt caaagtttgc
      901 accaataact tctcccactt cagctctcag acgaagaaga gtctctggat tgtacacgca
      961 gaatcccgga tgcatctcaa agcaaatctt tgtcacacca tgttcaagag caaactttcc
     1021 tgtctccttc cagtatggaa caagcacctc attccactga taatccagaa tctttaagaa
     1081 atcctccggc cagggacacg ttacccagtt tggatatttt gaatcagggc tgtctccagg
     1141 acatcctgaa aacgcaataa tcttttctac tccaagtttt tcagccagca gtatggcgtt
     1201 gataaagtca tcatggtact gctttgcaat ttccttgtct ggatgcaccg ggtttccgtg
     1261 gcatgccaag gcagaaattt caagtccatt gctgtaaatg gcgtccttga actcttcaag
     1321 agccttttca tccttcaaaa gttcctcagg attgcaatga gctttacctg gaaaaccgcc
     1381 gcaacctatc tccacggact gtacgccttc ctttgccagg aatgccattg cgtcccttac
     1441 gctcatgtca gacaacaata ctgttagtac tcctaacttc atagcataat cctcctcatg
//
